title: Biocompatibility and structural stability of a laminin biopolymer.
abstract: Polylaminin (polyLM) is a polymerized form of the extracellular matrix protein laminin obtained upon pH acidification. Here microscopy and spectroscopic tools are used to study the cell compatibility and the structural stability of polyLM, aiming at establishing its robustness as a biopolymer for therapeutic use. PolyLM is cell compatible as judged by the efficiency of attachment and neuritogenesis. It is resistant to low temperature. Addition of urea or an increase in hydrostatic pressure leads to polymer disassembly. PolyLM biofilms remain stable for 48 h in contact with cell culture medium. The sedimented polymer recovered after centrifugation and adsorbed on a glass coverslip preserved its original structure and its biological properties.
authors: Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors: Elisabete
authors: E
authors: Freire
authors: Madalena Martins Sant'Ana
authors: MM
authors: Barroso
authors: Richard Norman
authors: RN
authors: Klier
authors: Tatiana
authors: T
authors: Coelho-Sampaio
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D061251:Primary Cell Culture; D055550:Protein Stability; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D007797:Laminin