title: Transferred antigen-specific T(H)17 but not T(H)1 cells induce crescentic glomerulonephritis in mice.
abstract: To explore the role of antigen-specific CD4(+) T cells in glomerulonephritis, we administered ovalbumin 323-339 peptide conjugated to glomerular-binding polyclonal antibody and induced disease in RAG1(-/-) mice with CD4(+) T cells from OT2 × RAG1(-/-) mice. These OT2 × RAG1(-/-) mice have a transgenic T-cell receptor specific for this peptide. When CD4(+) T cells were primed in vivo, crescentic glomerulonephritis developed after 21 days in mice given peptide-conjugated glomerular-binding antibody but not unconjugated antibody control. We then investigated the relative roles of T(H)1 and T(H)17 cells, using Fab(2) fragments of glomerular-binding antibody to exclude a role for antibody in this model. T cells from OT2 × RAG1(-/-) mice were polarized in vitro, and T(H)1 or T(H)17 cell lines were injected into mice that were also given peptide-conjugated Fab(2) or unconjugated Fab(2) control, giving four experimental groups. After 21 days crescentic glomerulonephritis was seen in mice receiving T(H)17 cells and peptide-conjugated Fab(2) but in none of the other three groups. These results suggest that T(H)17 but not T(H)1 cells can induce crescentic glomerulonephritis.
authors: MRC Centre for Transplantation, King's College London School of Medicine, London, England.
authors: Calogero
authors: C
authors: Tulone
authors: Angela
authors: A
authors: Giorgini
authors: Simon
authors: S
authors: Freeley
authors: Alice
authors: A
authors: Coughlan
authors: Michael Gregory
authors: MG
authors: Robson
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005921:Glomerulonephritis / Q000276:immunology*; D018398:Homeodomain Proteins / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology; D018417:Th1 Cells / Q000276:immunology / Q000502:physiology*; D058504:Th17 Cells / Q000276:immunology / Q000502:physiology*
chemical_list: D016207:Cytokines; D018398:Homeodomain Proteins; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; C064658:RAG-1 protein