title: Analysis and comparison of glucocerebroside species from three edible sea cucumbers using liquid chromatography-ion trap-time-of-flight mass spectrometry.
abstract: Sphingolipids constitute a highly diverse and complex class of molecules and exhibit important physiological functions. Glucocerebrosides are anticipated to play a positive role in human nutrition. In this study, complicated glucocerebrosides from three specimens of edible sea cucumbers, specifically, Acaudina molpadioides, Cucumaria frondosa, and Apostichopus japonicus, were rapidly identified using liquid chromatography-ion trap-time-of-flight mass spectrometry (LCMS-IT-TOF), which is a powerful analysis tool. [M + H](+), [M + Na](+), and [M + H - H(2)O](+) in positive electrospray ionization (ESI) mode were used for MS/MS analysis to obtain product ion spectra. Various long-chain bases of glucocerebrosides were found in these sea cucumbers. Two of the most common long-chain bases were 2-amino-1,3-dihydroxy-4-heptadecene (d17:1) and 4,8-sphingadienine (d18:2), which were acylated to form saturated and monounsaturated nonhydroxy and monohydroxy fatty acids with 18-25 carbon atoms. The glucocerebroside molecular species were the most complicated in the sea cucumber C. frondosa and were the simplest in the sea cucumber A. molpadioides.
authors: College of Food Science and Engineering, Ocean University of China, No 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
authors: Jie
authors: J
authors: Xu
authors: Jingjing
authors: J
authors: Duan
authors: Changhu
authors: C
authors: Xue
authors: Tingyu
authors: T
authors: Feng
authors: Ping
authors: P
authors: Dong
authors: Tatsuya
authors: T
authors: Sugawara
authors: Takashi
authors: T
authors: Hirata
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005963:Glucosylceramides / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D012616:Sea Cucumbers / Q000737:chemistry*; D017747:Seafood / Q000032:analysis*
chemical_list: D005963:Glucosylceramides