title: Interaction between ROS and p38MAPK contributes to chemical hypoxia-induced             injuries in PC12 cells.
abstract: The present study investigated whether there is an interaction between reactive oxygen species (ROS) and p38 mitogen-activated protein kinase (MAPK) during chemical hypoxia-induced injury in PC12 cells. The results of the present study showed that cobalt chloride (CoCl₂), a chemical hypoxia agent, markedly induced ROS generation and phosphorylation of p38MAPK, as well as neuronal injuries. N-acetylcysteine (NAC), a ROS scavenger, blocked CoCl₂-induced phosphorylation of p38MAPK. In addition, SB203580, an inhibitor of p38MAPK attenuated not only CoCl₂-induced activation of p38MAPK, but also ROS production. These results suggest that ROS and p38MAPK are capable of interacting positively during chemical hypoxia. Furthermore, NAC and SB203580 markedly prevented CoCl₂-induced cytotoxicity, apoptosis and a loss of mitochondrial membrane potential. Taken together, our findings suggest that the positive interaction between CoCl₂ induction of ROS and p38MAPK activation may play a significant role in CoCl₂-induced neuronal injuries. We provide new insights into the mechanisms responsible for CoCl₂-induced injuries in PC12 cells.
authors: Department of Physiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, PR China.
authors: Ai-Ping
authors: AP
authors: Lan
authors: Liang-Can
authors: LC
authors: Xiao
authors: Zhan-Li
authors: ZL
authors: Yang
authors: Chun-Tao
authors: CT
authors: Yang
authors: Xiu-Yu
authors: XY
authors: Wang
authors: Pei-Xi
authors: PX
authors: Chen
authors: Mo-Fa
authors: MF
authors: Gu
authors: Jian-Qiang
authors: JQ
authors: Feng
mesh_terms: D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial; D016716:PC12 Cells; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D017382:Reactive Oxygen Species; D003035:Cobalt; D048051:p38 Mitogen-Activated Protein Kinases; C018021:cobaltous chloride; C093642:SB 203580