title: Complement-dependent lysis of influenza a virus-infected cells by broadly cross-reactive human monoclonal antibodies.
abstract: We characterized human monoclonal antibodies (MAbs) cloned from influenza virus-infected patients and from influenza vaccine recipients by complement-dependent lysis (CDL) assay. Most MAbs active in CDL were neutralizing, but not all neutralizing MAbs can mediate CDL. Two of the three stalk-specific neutralizing MAbs tested were able to mediate CDL and were more cross-reactive to temporally distant H1N1 strains than the conventional hemagglutination-inhibiting and neutralizing MAbs. One of the stalk-specific MAbs was subtype cross-reactive to H1 and H2 hemagglutinins, suggesting a role for stalk-specific antibodies in protection against influenza illness, especially by a novel viral subtype which can cause pandemics.
authors: Center for Infectious Disease and Vaccine Research, Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA. Masanori.Terajima@umassmed.edu
authors: Masanori
authors: M
authors: Terajima
authors: John
authors: J
authors: Cruz
authors: Mary Dawn T
authors: MD
authors: Co
authors: Jane-Hwei
authors: JH
authors: Lee
authors: Kaval
authors: K
authors: Kaur
authors: Jens
authors: J
authors: Wrammert
authors: Patrick C
authors: PC
authors: Wilson
authors: Francis A
authors: FA
authors: Ennis
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D057134:Antibodies, Neutralizing / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D016923:Cell Death; D003165:Complement System Proteins / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D003165:Complement System Proteins