title: [Comparison of whole genome sequences and replication ability in cell cultures between two avian leukosis viruses of subgroup B].
abstract: The purpose of this study was to compare the whole genome sequences and replication dynamics in cell cultures of two Avian leukosis viruses of subgroup B (ALV) isolates, SDAU09E3 and SDAU09C2. Comparison of the amino acid sequences indicated that the gp85 identity of these two subgroup B isolates was 95.4%, the identity with other three ALV-B reference strains was 91.0%-94.9%, and less than 87.9% with ALV subgroup A, C, D, E and J. Comparison of the nucleotide sequence of gag and pol genes indicated that homologies of gag gene and pol gene of these two ALV-B isolates with all compared reference strains of different subgroups were above 93%. Homologies of LTR sequence of these two ALV-B isolates with other exogenous ALVs subgroups A, B, C, D and J were 72.6%-88.3%, but only 51.5% when compared with endogenous ALV subgroup E. The identity of LTR between these two ALV-B strains was only 74.8%, which was far lower than the identity of other genes. The identity of U3 region of LTR between these two ALV-B isolates was only 68.8% and there were obvious differences in the number CAAT Boxes. Replication dynamics in DF-1 cell indicated that the value of TCID50 was similar between 2 isolates but the concentration of nucleocapsid protein p27 antigen of SDAU09E3 was significantly higher than SDAU09C2 in cell culture supernatant, which indicated there was no parallel relationship between p27 antigen concentration and infectious virus particles. Whether such difference was resulted from the diversity of U3 region of LTR, further studies with their recombinant infectious clones is necessary.
authors: College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China. wujichang19861213@163.com
authors: Zhuan-Chang
authors: ZC
authors: Wu
authors: Mei-Zhen
authors: MZ
authors: Zhu
authors: Xiao-Ming
authors: XM
authors: Bian
authors: Cheng-Tai
authors: CT
authors: Ma
authors: Peng
authors: P
authors: Zhao
authors: Zhi-Zhong
authors: ZZ
authors: Cui
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001354:Avian Leukosis Virus / Q000145:classification / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014763:Viral Matrix Proteins / Q000235:genetics; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral; D014763:Viral Matrix Proteins