title: A new recombinant Orf virus (ORFV, Parapoxvirus) protects rabbits against lethal infection with rabbit hemorrhagic disease virus (RHDV).
abstract: This report describes the generation of a new recombinant Orf virus (ORFV; Parapoxvirus) expressing the major capsid protein VP1 (VP60) of the calicivirus, rabbit hemorrhagic disease virus (RHDV). Authentic expression of VP1 could be demonstrated in cells infected with the recombinant D1701-V-VP1 without the need for production of infectious ORFV progeny. Notably, infected cells also released empty calicivirus-like particles (VLPs). Challenge experiments showed that even a single immunization with ≥10(5) PFU of D1701-V-VP1 protected rabbits against lethal RHDV infection. ELISA tests indicated that the protective immunity mediated by D1701-V-VP1 did not strictly depend on the presence of detectable RHDV-specific serum antibodies. The induction of interleukin-2 found only in the sera of rabbits immunized with the D1701-V-VP1, but not in sera of rabbits immunized with the inactivated commercial vaccine RIKA-VACC, might indicate also some involvement of T-cells in protection. Collectively, this work adds another example of the successful use of the ORFV vector system for the generation of a recombinant vaccine, and demonstrates its potential as an alternative vaccine to protect rabbits against RHDV infection.
authors: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Immunology, Paul-Ehrlich-Str. 28, D-72074 Tuebingen, FRG.
authors: Joerg
authors: J
authors: Rohde
authors: Horst
authors: H
authors: Schirrmeier
authors: Harald
authors: H
authors: Granzow
authors: Hanns-Joachim
authors: HJ
authors: Rziha
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D017250:Caliciviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology*; D008297:Male; D009923:Orf virus / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D014709:Vero Cells; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus