title: Localization-based super-resolution microscopy with an sCMOS camera.
abstract: In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region.
authors: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China. sqzeng@mail.hust.edu.cn
authors: Zhen-Li
authors: ZL
authors: Huang
authors: Hongyu
authors: H
authors: Zhu
authors: Fan
authors: F
authors: Long
authors: Hongqiang
authors: H
authors: Ma
authors: Lingsong
authors: L
authors: Qin
authors: Yongfeng
authors: Y
authors: Liu
authors: Jiuping
authors: J
authors: Ding
authors: Zhihong
authors: Z
authors: Zhang
authors: Qingming
authors: Q
authors: Luo
authors: Shaoqun
authors: S
authors: Zeng
mesh_terms: D004867:Equipment Design; D008853:Microscopy / Q000295:instrumentation*; D017785:Photons; D012666:Semiconductors; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*