title: Review: applications of mass spectrometry techniques in the investigation of milk proteome.
abstract: The introduction of "soft" desorption/ionization methods such as electrospray ionization and matrix-assisted laser desorption/ionization has determined a breakthrough in the application of mass spectrometry to the structural analysis of proteins. The contemporary advancement of bioinformatics, together with the possibility to combine these mass spectrometric methods with electrophoretic or chromatographic separation techniques has opened up the new field of proteome analysis and, more generally, has established these approaches as indispensable tools for protein and peptide analysis in complex mixtures, such as milk and milk- derived foods. Here, a necessarily not exhaustive series of current applications of mass spectrometry-based techniques for the characterization of milk proteins will be summarized. These include the characterization of milk protein polymorphism, determination of the structural modifications induced on milk proteins by industrial processes, investigation of milk adulterations and characterization of milk allergens.
authors: Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Catania, Italy. vcunsolo@unict.it
authors: Vincenzo
authors: V
authors: Cunsolo
authors: Vera
authors: V
authors: Muccilli
authors: Rosaria
authors: R
authors: Saletti
authors: Salvatore
authors: S
authors: Foti
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D019295:Computational Biology / Q000379:methods; D003611:Dairy Products; D005506:Food Contamination; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis* / Q000737:chemistry; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000485:Allergens; D008894:Milk Proteins; D010455:Peptides; D020543:Proteome