title: Fluoridized iron phosphate as a novel adsorbent for selective separation/isolation of cytochrome c.
abstract: Fluoridized iron phosphate (F-FePO) is prepared via a hydrothermal protocol and characterized by means of (57)Fe Mössbauer spectra, Fourier transform infrared, and surface charge analysis. For the first time, F-FePO is used as an adsorbent for the adsorption of proteins, which exhibits favorable selectivity toward cytochrome c (cyt-c) in the presence of acidic and neutral proteins under controlled experimental conditions. At pH 10.5, an adsorption efficiency of 100% is achieved for 60 mg L(-1) cyt-c in 1.0 mL of sample solution using 6.0 mg F-FePO. The adsorption behavior is consistent with the Langmuir adsorption model, corresponding to a theoretical adsorption capacity of 37.59 μg mg(-1). The retained cyt-c on F-FePO could be readily collected by 0.1 mol L(-1) Na(2)CO(3)-NaHCO(3) buffer (pH 10.5), deriving a recovery of 100%. Circular dichroism spectra indicate no conformational change for cyt-c after the adsorption and desorption processes, demonstrating the favorable biocompatibility of the fluoridized iron phosphate. F-FePO is employed for the selective isolation of cyt-c from a spiked human whole blood, achieving satisfactory results by assay with SDS-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis.
authors: Research Center for Analytical Sciences, Northeastern University, Shenyang, China.
authors: Shisong
authors: S
authors: Tang
authors: WenJing
authors: W
authors: Wang
authors: XuWei
authors: X
authors: Chen
authors: JianHua
authors: J
authors: Wang
mesh_terms: D000327:Adsorption; D045304:Cytochromes c / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D005290:Ferric Compounds; D045304:Cytochromes c; C035885:ferric phosphate