title: Presence of infectious RD-114 virus in a proportion of canine parvovirus isolates.
abstract: We recently found that certain canine live attenuated vaccines produced using `non-feline' cell lines were contaminated with an infectious feline endogenous retrovirus, termed RD-114 virus. We suspected that RD-114 virus may have contaminated the seed stock of canine parvovirus (CPV) during the production of the contaminated vaccines. In this study, we collected stock viruses of CPVs propagated in a feline cell line, and checked the presence of infectious RD-114 virus. Consequently, we found that RD-114 viral RNA was present in all stock viruses, and 7 out of 18 stock viruses were contaminated with infectious RD-114 virus. We also found that RD-114 virus was stable physically and is capable of retaining its infectivity for a long period at -80°C.
authors: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto 606–8507, Japan.
authors: Rokusuke
authors: R
authors: Yoshikawa
authors: Eiji
authors: E
authors: Sato
authors: Takayuki
authors: T
authors: Miyazawa
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D004285:Dogs; D004340:Drug Contamination; D020077:Endogenous Retroviruses / Q000145:classification / Q000235:genetics*; D017993:Parvovirus, Canine / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000235:genetics; D014765:Viral Vaccines / Q000032:analysis*
chemical_list: D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines