title: Hispidin isolated from Phellinus linteus protects against hydrogen peroxide-induced oxidative stress in pancreatic MIN6N β-cells.
abstract: Reactive oxygen species (ROS) have been shown to cause DNA damage, protein denaturation, loss of antioxidative enzyme activity, and lipid peroxidation. Thus, ROS are associated with tissue damage and are considered to be prime contributing factors in inflammation, diabetes, aging, and cancer. In this study, we investigated whether or not hispidin protects pancreatic MIN6N β-cells from oxidative stress caused by hydrogen peroxide. Treatment of MIN6N β-cells with 0.5 mM hydrogen peroxide for 4 hours caused significant loss of cell viability and an increase in the number of apoptotic cells. However, pretreatment of MIN6N β-cells with hispidin for 24 hours reduced loss of cell viability and decreased the number of apoptotic cells. In addition, 70 μM hispidin significantly scavenged intracellular ROS and inhibited apoptosis and caspase-3 induced by hydrogen peroxide. Furthermore, the generation of thiobarbituric acid-reactive substances was inhibited in the presence of hispidin in a dose-dependent manner. Also, 70 μM hispidin significantly increased insulin secretion in hydrogen peroxide-treated MIN6N β-cells. These results suggest that hispidin may be effective for protecting MIN6N β-cells from ROS toxicity in diabetes.
authors: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, Korea.
authors: Jung Hyun
authors: JH
authors: Lee
authors: Jong Seok
authors: JS
authors: Lee
authors: Young Rae
authors: YR
authors: Kim
authors: Woo Chul
authors: WC
authors: Jung
authors: Keun Eok
authors: KE
authors: Lee
authors: Shin Young
authors: SY
authors: Lee
authors: Eock Kee
authors: EK
authors: Hong
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000494:pharmacology*; D011753:Pyrones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007328:Insulin; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D011753:Pyrones; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide; C505407:CASP3 protein, human; D053148:Caspase 3; C007946:hispidin