title: Effects of nonylphenol on aldosterone release from rat zona glomerulosa cells.
abstract: Alkylphenol ethoxylate, which consists of approximately 80% nonylphenol ethoxylate (NPE), is a major nonionic surfactant. Nonylphenol (NP), the primary degradation product of NPE, has been reported to interfere with reproduction in fish, reptiles, and mammals by inducing cell death in the gonads and by affecting other reproductive parameters. However, the effects of NP on rat adrenal zona glomerulosa cells (ZG) and the underlying mechanisms remain unclear. In this study, we explored the effects of NP on aldosterone release. ZG cells were incubated with NP in the presence or absence of the secretagogues angiotensin II (ANG II), potassium, 8-Br-cAMP, 25-OH-cholesterol, corticosterone or cyclopiazonic acid (CPA). After performing radioimmunoassay (RIA) and Western blot analysis, we found that (1) NP stimulated aldosterone release in cells induced by ANG II, KCl, 8-Br-cAMP, 25-OH-cholesterol, corticosterone, and CPA; (2) NP triggered the release of higher amounts of pregnenolone in cells treated with vehicle and 25-OH-cholesterol+trilostane than in cells treated with other compounds; and (3) the stimulatory effect of NP seemed to be mediated through steroidogenic acute regulatory protein (StAR) and aldosterone synthase activity. These observations suggest that the effects of NP are mediated via increased free Ca(2+) in the cytoplasm.
authors: Department of Chemical and Materials Engineering, Chinese Culture University, Taipei, Taiwan, ROC.
authors: Ling-Ling
authors: LL
authors: Chang
authors: Wan-Song Alfred
authors: WS
authors: Wun
authors: Paulus S
authors: PS
authors: Wang
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003345:Corticosterone / Q000494:pharmacology; D019405:Cytochrome P-450 CYP11B2 / Q000378:metabolism; D013196:Dihydrotestosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D006888:Hydroxycholesterols / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011284:Pregnenolone / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014874:Water Pollutants, Chemical / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000166:cytology / Q000187:drug effects*; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D006888:Hydroxycholesterols; D007211:Indoles; D010636:Phenols; D010750:Phosphoproteins; D014874:Water Pollutants, Chemical; D000098682:Steroidogenic Acute Regulatory Protein; D013196:Dihydrotestosterone; D000804:Angiotensin II; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000450:Aldosterone; D011284:Pregnenolone; C025256:nonylphenol; D019405:Cytochrome P-450 CYP11B2; C009954:trilostane; D011188:Potassium; D002118:Calcium; D003345:Corticosterone; C000543:cyclopiazonic acid