title: An in situ electrochemical cell for Q- and W-band EPR spectroscopy.
abstract: A simple design for an in situ, three-electrode spectroelectrochemical cell is reported that can be used in commercial Q- and W-band (ca. 34 and 94 GHz, respectively) electron paramagnetic resonance (EPR) spectrometers, using standard sample tubing (1.0 and 0.5 mm inner diameter, respectively) and within variable temperature cryostat systems. The use of the cell is demonstrated by the in situ generation of organic free radicals (quinones and diimines) in fluid and frozen media, transition metal ion radical anions, and on the enzyme nitric oxide synthase reductase domain (NOSrd), in which a pair of flavin radicals are generated.
authors: School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, West Mains Road, Edinburgh EH9 3JJ, UK.
authors: Paul R
authors: PR
authors: Murray
authors: David
authors: D
authors: Collison
authors: Simon
authors: S
authors: Daff
authors: Nicola
authors: N
authors: Austin
authors: Ruth
authors: R
authors: Edge
authors: Brian W
authors: BW
authors: Flynn
authors: Lorna
authors: L
authors: Jack
authors: Fanny
authors: F
authors: Leroux
authors: Eric J L
authors: EJ
authors: McInnes
authors: Alan F
authors: AF
authors: Murray
authors: Daniel
authors: D
authors: Sells
authors: Tom
authors: T
authors: Stevenson
authors: Joanna
authors: J
authors: Wolowska
authors: Lesley J
authors: LJ
authors: Yellowlees
mesh_terms: D016880:Anisotropy; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004572:Electrolysis; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation*; D005486:Flavin Mononucleotide / Q000032:analysis; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis; D005609:Free Radicals / Q000032:analysis; D005615:Freezing; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry; D011725:Pyridines / Q000032:analysis; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
chemical_list: D005609:Free Radicals; D007202:Indicators and Reagents; D045322:Photosynthetic Reaction Center Complex Proteins; D011725:Pyridines; D014451:Ubiquinone; D005182:Flavin-Adenine Dinucleotide; D005486:Flavin Mononucleotide; D010088:Oxidoreductases; C034747:nitric-oxide reductase