title: Ω3-Polyunsaturated fatty acids prevent lipoperoxidation, modulate antioxidant enzymes, and reduce lipid content but do not alter glycogen metabolism in the livers of diabetic rats fed on a high fat thermolyzed diet.
abstract: Ω3-Polyunsaturated fatty acids (Ω3-PUFAs) are known to act as hypolipidaemics, but the literature is unclear about the effects that Ω3-PUFAs have on oxidative stress in obese and diabetic patients. In this study, our aim was to investigate the effects of Ω3-PUFAs on oxidative stress, including antioxidant enzyme activity and hepatic lipid and glycogen metabolism in the livers of diabetic and non-diabetic rats fed on a high fat thermolyzed diet. Rats were divided into six groups: (1) the control group (C), (2) the control diabetic group (D), (3) the high fat thermolyzed diet group (HFTD), which were fed a diet that was enriched in fat that was heated for 60 min at 180°C, (4) the high fat thermolyzed diet diabetic group (D + HFTD), (5) the high fat thermolyzed diet + Ω3 polyunsaturated fatty acid group (HFTD + Ω3), and (6) the high fat thermolyzed diet + Ω3 polyunsaturated fatty acid diabetic group (D + HFTD + Ω3). The most important finding of this study was that Ω3-PUFAs are able to reduce triglycerides, non-esterified fatty acid, lipoperoxidation levels, advanced glycation end products, SOD/CAT enzymatic ratio, and CAT immunocontent and increase SOD2 levels in the livers of diabetic rats fed with a HFTD. However, Ω3-PUFAs did not alter the observed levels of protein damage, blood glucose, or glycogen metabolism in the liver. These findings suggest that Ω3-PUFAs may represent an important auxiliary adjuvant in combating some diseases like diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease.
authors: Programa de Pós graduação em Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. amassis@terra.com.br
authors: Adriano M
authors: AM
authors: de Assis
authors: Anderson
authors: A
authors: Rech
authors: Aline
authors: A
authors: Longoni
authors: Liane N
authors: LN
authors: Rotta
authors: Cristiane C
authors: CC
authors: Denardin
authors: Matheus A
authors: MA
authors: Pasquali
authors: Diogo O
authors: DO
authors: Souza
authors: Marcos L S
authors: ML
authors: Perry
authors: José C
authors: JC
authors: Moreira
mesh_terms: D050154:Adiposity; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D059305:Diet, High-Fat; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D017127:Glycation End Products, Advanced / Q000097:blood; D006003:Glycogen / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D017127:Glycation End Products, Advanced; D017392:Thiobarbituric Acid Reactive Substances; C048496:N(6)-carboxymethyllysine; D006003:Glycogen; D002374:Catalase; D013482:Superoxide Dismutase; D008239:Lysine