title: Potentially predictive microRNAs of gastric cancer with metastasis to lymph node.
abstract: OBJECTIVE
  To detect the expression of 60 microRNAs (miRNAs) in gastric cancer tissues and find new predictive biomarkers of gastric cancer with metastasis.
  
  
  METHODS
  The expressions of 60 candidate miRNAs in 30 gastric cancer tissues and paired normal tissues were detected by stem-loop real-time reverse transcription-polymerase chain reaction. After primary screening of miRNAs expression, 5 selected miRNAs were further testified in another 22 paired gastric tissues. Based on the expression level of miRNAs and the status of metastasis to lymph node (LN), receiver-operating-characteristic (ROC) curve were used to evaluate their ability in predicting the status of metastasis to LN.
  
  
  RESULTS
  Thirty-eight miRNAs expressions in gastric cancer tissues were significantly different from those in paired normal tissues (P < 0.01). Among them, 31 miRNAs were found to be up-expressed in cancer tissues and 1 miRNAs were down-expressed ≥ 1.5 fold vs paired normal gastric tissue. Five microRNAs (miR-125a-3p, miR-133b, miR-143, miR-195 and miR-212) were differently expressed between different metastatic groups in 30 gastric cancer biopsies (P < 0.05). Partial correlation analysis showed that hsa-mir-212 and hsa-mir-195 were correlated with the status of metastasis to LN in spite of age, gender, tumor location, tumor size, depth of invasion and cell differentiation. ROC analysis indicated that miR-212 and miR-195 have better sensitivities (84.6% and 69.2%, respectively) and specificities (both 100%) in distinguishing biopsies with metastasis to LN from biopsies without metastasis to LN.
  
  
  CONCLUSIONS
  miR-212 and miR-195 could be independent biomarkers in predicting the gastric cancer with metastasis to LN.
authors: Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China.
authors: Wen-Yi
authors: WY
authors: Wu
authors: Xiang-Yang
authors: XY
authors: Xue
authors: Zhe-Jing
authors: ZJ
authors: Chen
authors: Shao-Liang
authors: SL
authors: Han
authors: Ying-Peng
authors: YP
authors: Huang
authors: Li-Fang
authors: LF
authors: Zhang
authors: Guan-Bao
authors: GB
authors: Zhu
authors: Xian
authors: X
authors: Shen
mesh_terms: D020869:Gene Expression Profiling; D005819:Genetic Markers; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D012372:ROC Curve; D013274:Stomach Neoplasms / Q000235:genetics* / Q000556:secondary*
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs