title: FT-NIR spectroscopy for rapid and simple determination of nimesulide in rabbit plasma for pharmacokinetic analysis.
abstract: High-throughput analysis of a large number of samples for pharmacokinetic study is necessary in drug development and pharmacovigilance. Usually, drug quantification for pharmacokinetics and bio-availability is achieved through matrix extraction and HPLC analysis, which is time, labour and cost intensive method. A prompt and solvent free method is the quest for such analysis in the present times. Pharmacokinetic analysis of nimesulide from plasma samples of rabbits through Fourier transform near infrared (FT-NIR) spectroscopy analysis combined with partial least squares (PLS) regression model was undertaken with validation through HPLC analysis. Pharmacokinetic parameters obtained through FT-NIR and HPLC were found to be statistically similar with errors below the acceptable limits. The study demonstrates the use of FT-NIR for pharmacokinetics and bio-availability studies. This high throughput method analyses more than 50 samples in an hour without solvents usage and provide ample scope for automation and commercial utilization.
authors: Analytical Chemistry Department, CSIR - Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow 226015, India. pvajayan.cimap@yahoo.com
authors: P V
authors: PV
authors: Ajayakumar
authors: Debabrata
authors: D
authors: Chanda
authors: Anirban
authors: A
authors: Pal
authors: Mahendra P
authors: MP
authors: Singh
authors: A
authors: A
authors: Samad
mesh_terms: D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D016018:Least-Squares Analysis; D008297:Male; D011817:Rabbits; D012044:Regression Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013449:Sulfonamides / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D013449:Sulfonamides; C012655:nimesulide