title: Polyhydroxyalkanoates production from cellulose hydrolysate in Escherichia coli LS5218 with superior resistance to 5-hydroxymethylfurfural.
abstract: Poly[3-hydroxybutyrate-co-3-hydroxyvalerate(3HV)] was produced in recombinant Escherichia coli LS5218 from ruthenium-catalyzed cellulose hydrolysate and propionate. The strain was found to be resistant to 5-hydroxymethylfurfural (5-HMF), which is a major inhibitory byproduct generated in the cellulose hydrolysis reaction. The 3HV fraction was successfully regulated in the range of 5.6-40 mol%.
authors: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
authors: John Masani
authors: JM
authors: Nduko
authors: Wakako
authors: W
authors: Suzuki
authors: Ken'ichiro
authors: K
authors: Matsumoto
authors: Hirokazu
authors: H
authors: Kobayashi
authors: Toshihiko
authors: T
authors: Ooi
authors: Atsushi
authors: A
authors: Fukuoka
authors: Seiichi
authors: S
authors: Taguchi
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology; D006868:Hydrolysis; D007218:Industrial Microbiology; D011091:Polyesters / Q000378:metabolism*; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011422:Propionates / Q000378:metabolism; D012428:Ruthenium; D000094122:Polyhydroxybutyrates
chemical_list: D002482:Cellulose; D005662:Furaldehyde; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011422:Propionates; D012428:Ruthenium; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C008046:5-hydroxymethylfurfural