title: Microarray profiling reveals microRNAs involving soybean resistance to Phytophthora sojae.
abstract: MicroRNAs (miRNAs), a group of small noncoding RNAs, may serve as a class of post-transcriptional regulators in plant immune systems. Nevertheless, little is known about their roles in plant immune response to the oomycete pathogens. To identify miRNAs involved in the response of soybean to Phytophthora sojae, we examined expressional patterns of miRNAs upon infection by P. sojae by microarray analysis in three soybean cultivars: Williams (susceptible), Conrad (quantitative resistance), and Williams 82 (qualitative resistance). Expression of a number of miRNAs was significantly altered upon infection and (or) in the different genotypes. qRT-PCR data with some miRNAs further confirmed the microarray results. Comparative analysis of the selected miRNAs and their targeted gene expression datasets uncovered many reciprocally expressed miRNA-target pairs, which could proposed a feedback circuit between miRNA(s) and protein-coding genes. These results may serve as a basis for further in-depth studies of miRNAs involved in soybean resistance to P. sojae.
authors: a National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors: Na
authors: N
authors: Guo
authors: Wen-Wu
authors: WW
authors: Ye
authors: Xiao-Ling
authors: XL
authors: Wu
authors: Dan-Yu
authors: DY
authors: Shen
authors: Yuan-Chao
authors: YC
authors: Wang
authors: Han
authors: H
authors: Xing
authors: Dao-Long
authors: DL
authors: Dou
mesh_terms: D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010838:Phytophthora / Q000276:immunology / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics / Q000276:immunology* / Q000382:microbiology
chemical_list: D035683:MicroRNAs