title: High-content screening of peptide-based non-viral gene delivery systems.
abstract: High-content screening (HCS) uses high-capacity automated fluorescence imaging for the quantitative analysis of single cells and cell populations. Here, we developed an HCS assay for rapid screening of non-viral gene delivery systems as exemplified by the screening of a small library of peptide-based transfectants. These peptides were simultaneously screened for transfection efficiency, cytotoxicity, induction of cell permeability and the capacity to transfect non-dividing cells. We demonstrated that HCS is a valuable extension to the already existing screening methods for the in vitro evaluation of non-viral gene delivery systems with the added value that multiple parameters can be screened in parallel thereby obtaining more information from a single screening event, which will accelerate the development of novel gene delivery systems.
authors: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of science, University of Utrecht, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands.
authors: Markus
authors: M
authors: de Raad
authors: Erik A
authors: EA
authors: Teunissen
authors: Daphne
authors: D
authors: Lelieveld
authors: David A
authors: DA
authors: Egan
authors: Enrico
authors: E
authors: Mastrobattista
mesh_terms: D000818:Animals; D019556:COS Cells; D002452:Cell Count; D002463:Cell Membrane Permeability; D002470:Cell Survival; D002522:Chlorocebus aethiops; D057166:High-Throughput Screening Assays; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010455:Peptides; D014162:Transfection
chemical_list: D019151:Peptide Library; D010455:Peptides