title: Antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian medicinal plant Eremophila neglecta.
abstract: OBJECTIVE
  To determine the antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian plant Eremophila neglecta.
  
  
  RESULTS
  Antimicrobial activities of serrulatane compounds 8,19-dihydroxyserrulat-14-ene (1) and 8-hydroxyserrulat-14-en-19-oic acid (2) were tested against Gram-negative and Gram-positive bacteria including human and veterinary pathogens and some multidrug-resistant isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the compounds were determined by broth microdilution assay. Both compounds exhibited antibacterial activity against all Gram-positive test strains. They showed antimycobacterial activity against isolates of Mycobacterium fortuitum and Mycobacterium chelonae. Of the five Gram-negative bacteria tested, only Moraxella catarrhalis showed susceptibility to the compounds. Cytotoxic activities were tested in the Vero cell line. Compound 1 showed more activity than 2 in both antibacterial and cytotoxicity assays with cytotoxicity at concentrations similar to the MBC.
  
  
  CONCLUSIONS
  Serrulatane compounds showed significant activity against medically important bacteria, with 1 exhibiting stronger antibacterial activity. However, they also displayed toxicity to mammalian cells.
  
  
  CONCLUSIONS
  Serrulatanes are of interest as novel antibacterial compounds for use in biomedical applications; this study reports data obtained with a range of bacterial strains and mammalian cells, essential for assessing the capabilities and limitations of potential applicability of these compounds.
authors: School of Pharmacy and Medical Sciences & Sansom Institute for Health Research, University of South Australia, Adelaide, SA, Australia.
authors: O F
authors: OF
authors: Anakok
authors: C P
authors: CP
authors: Ndi
authors: M D
authors: MD
authors: Barton
authors: H J
authors: HJ
authors: Griesser
authors: S J
authors: SJ
authors: Semple
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001315:Australia; D002470:Cell Survival / Q000187:drug effects*; D002522:Chlorocebus aethiops; D004224:Diterpenes / Q000494:pharmacology*; D041621:Eremophila Plant / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D014709:Vero Cells
chemical_list: C524230:8,19-dihydroxyserrulat-14-ene; C524231:8-hydroxyserrulat-14-en-19-oic acid; D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010936:Plant Extracts