title: Aberrant hippocampal spine morphology and impaired memory formation in neuronal platelet-derived growth factor β-receptor lacking mice.
abstract: The physiological role of platelet-derived growth factor (PDGF) in the central nervous system (CNS) synaptic function remains uncharacterized. Here we identify physiological roles of PDGF receptor-β (PDGFR-β) in the CNS by conditional knockout of the gene encoding it. In the hippocampus, PDGFR-β colocalized immunohistochemically with both presynaptic synaptophysin and postsynaptic density-95 (PSD-95). In the hippocampal CA1 region, expression levels of postsynaptic proteins, including spinophilin, drebrin, and PSD-95, were significantly decreased in PDGFR-β knockout mice, although presynaptic synaptophysin levels remained comparable to controls. Interestingly, in hippocampal CA1 pyramidal neurons, dendritic spine density in PDGFR-β knockout mice was significantly decreased compared with that seen in wild-type mice, although spine length and number of dendritic branches remained unchanged. Consistent with these findings, impairment in hippocampal long-term potentiation (LTP) and in hippocampus-dependent memory formation were seen in PDGFR-β knockout mice. These results suggest PDGFR-β plays critical roles in spine morphology and memory formation in mouse brain.
authors: Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors: Norifumi
authors: N
authors: Shioda
authors: Shigeki
authors: S
authors: Moriguchi
authors: Takeshi
authors: T
authors: Oya
authors: Yoko
authors: Y
authors: Ishii
authors: Jie
authors: J
authors: Shen
authors: Takako
authors: T
authors: Matsushima
authors: Hisao
authors: H
authors: Nishijo
authors: Masakiyo
authors: M
authors: Sasahara
authors: Kohji
authors: K
authors: Fukunaga
mesh_terms: D000818:Animals; D049229:Dendritic Spines / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008569:Memory Disorders / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000172:deficiency* / Q000235:genetics
chemical_list: D020797:Receptor, Platelet-Derived Growth Factor beta