title: Gene expression changes in steroid-induced IOP elevation in bovine trabecular meshwork.
abstract: OBJECTIVE
  To determine whether gene expression changes occur in the trabecular meshwork (TM) of cow eyes with steroid-induced intraocular pressure (IOP) elevation.
  
  
  METHODS
  Adult female Braford cows (n = 4) were subjected to uniocular prednisolone acetate treatment for 6 weeks. IOP was monitored with an applanation tonometer. At the conclusion of the experiment, animals were euthanized, eyes were enucleated, and the TM was dissected and stored in an aqueous nontoxic tissue storage reagent. RNA was extracted and subjected to microarray analysis using commercial oligonucleotide bovine arrays. Some of the genes differentially expressed between control and experimental eyes were confirmed by quantitative RT-PCR and some of the respective proteins were studied by immunoblotting.
  
  
  RESULTS
  IOP began to increase after 3 weeks of treatment, reaching a peak 2 weeks later. IOP differences between corticosteroid-treated and fellow control eyes were 6 ± 1 mm Hg (mean ± SD) at the conclusion of the study. Microarray analysis revealed that expression of 258 genes was upregulated, whereas expression of 187 genes was downregulated in the TM of eyes with steroid-induced IOP elevation. Genes identified to be differentially expressed include genes coding for cytoskeletal proteins, enzymes, growth and transcription factors, as well as extracellular matrix proteins and immune response proteins. A number of relevant gene networks were detected by bioinformatic analysis.
  
  
  CONCLUSIONS
  Steroid-induced IOP elevation alters gene expression in the bovine TM. Identification of genes with changing expression in this model of open-angle glaucoma may help elucidate the primary changes occurring at the molecular level in this condition.
authors: Department of Ophthalmology, Box 5, SUNY Downstate, 450 Clarkson Ave., Brooklyn, NY 11203, USA. john.danias@downstate.edu
authors: John
authors: J
authors: Danias
authors: Rosana
authors: R
authors: Gerometta
authors: Yongchao
authors: Y
authors: Ge
authors: Lizhen
authors: L
authors: Ren
authors: Lampros
authors: L
authors: Panagis
authors: Thomas W
authors: TW
authors: Mittag
authors: Oscar A
authors: OA
authors: Candia
authors: Steven M
authors: SM
authors: Podos
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002417:Cattle; D019295:Computational Biology; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D005902:Glaucoma, Open-Angle / Q000139:chemically induced* / Q000235:genetics* / Q000503:physiopathology; D007429:Intraocular Pressure / Q000187:drug effects / Q000502:physiology; D008957:Models, Genetic; D009798:Ocular Hypertension / Q000139:chemically induced* / Q000235:genetics* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D014129:Trabecular Meshwork / Q000502:physiology*
chemical_list: D000893:Anti-Inflammatory Agents; C009935:prednisolone acetate; D011239:Prednisolone