title: hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising             diagnostic marker in human bladder urothelial carcinomas.
abstract: Numerous microRNAs (miRNAs) play crucial roles in cancer development. In             this study, we report that hsa-miR-96 is expressed at higher levels in human bladder             urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased             invasion and differentiation of human bladder T24 cells and promoted their growth.             Down‑regulation of hsa-miR-96 significantly affected the phenotype of bladder             cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1             (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96             inhibitor when compared with levels in cells transfected with the empty plasmid             vector or the negative control miRNA inhibitor. Altogether, these results suggest             that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating             IRS1 and MAP4K1 levels, functioning as a promising diagnostic marker in human             bladder urothelial carcinomas.
authors: Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.
authors: Yi
authors: Y
authors: Wang
authors: Hongmei
authors: H
authors: Luo
authors: Yangle
authors: Y
authors: Li
authors: Tieding
authors: T
authors: Chen
authors: Shengpeng
authors: S
authors: Wu
authors: Luoyan
authors: L
authors: Yang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000175:diagnosis / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; C102102:hematopoietic progenitor kinase 1; D017346:Protein Serine-Threonine Kinases