title: Molecular mapping of Arabidopsis thaliana lipid-related orthologous genes in Brassica napus.
abstract: Quantitative Trait Loci (QTL) for oil content has been previously analyzed in a SG-DH population from a cross between a Chinese cultivar and a European cultivar of Brassica napus. Eight QTL with additive and epistatic effects, and with environmental interactions were evaluated. Here we present an integrated linkage map of this population predominantly based on informative markers derived from Brassica sequences, including 249 orthologous A. thaliana genes, where nearly half (112) are acyl lipid metabolism related genes. Comparative genomic analysis between B. napus and A. thaliana revealed 33 colinearity regions. Each of the conserved A. thaliana segments is present two to six times in the B. napus genome. Approximately half of the mapped lipid-related orthologous gene loci (76/137) were assigned in these conserved colinearity regions. QTL analysis for seed oil content was performed using the new map and phenotypic data from 11 different field trials. Nine significant QTL were identified on linkage groups A1, A5, A7, A9, C2, C3, C6 and C8, together explaining 57.79% of the total phenotypic variation. A total of 14 lipid related candidate gene loci were located in the confidence intervals of six of these QTL, of which ten were assigned in the conserved colinearity regions and felled in the most frequently overlapped QTL intervals. The information obtained from this study demonstrates the potential role of the suggested candidate genes in rapeseed kernel oil accumulation.
authors: Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. jyzhao3@yahoo.com
authors: Jianyi
authors: J
authors: Zhao
authors: Jixiang
authors: J
authors: Huang
authors: Fei
authors: F
authors: Chen
authors: Fei
authors: F
authors: Xu
authors: Xiyuan
authors: X
authors: Ni
authors: Haiming
authors: H
authors: Xu
authors: Yilong
authors: Y
authors: Wang
authors: Chonchon
authors: C
authors: Jiang
authors: Hao
authors: H
authors: Wang
authors: Aixia
authors: A
authors: Xu
authors: Ruizhi
authors: R
authors: Huang
authors: Dianrong
authors: D
authors: Li
authors: Jinling
authors: J
authors: Meng
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029688:Brassica napus / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics; D010641:Phenotype; D010938:Plant Oils / Q000032:analysis*; D040641:Quantitative Trait Loci / Q000235:genetics*; D013045:Species Specificity
chemical_list: D005819:Genetic Markers; D010938:Plant Oils