title: In vitro studies on degradation of synthetic dye mixture by Comamonas sp. VS-MH2 and evaluation of its efficacy using simulated microcosm.
abstract: Reactive azo dyes are considered as one of the most detrimental pollutants from industrial effluents and therefore their biodegradation is receiving constant scientific consideration. A bacterial isolate VS-MH2, originating from dye contaminated sites of Gujarat, India, was exploited for its ability to degrade a synthetic dye mixture (SDM) (comprising of four azo reactive dyes) under static conditions. The identification of the isolate by 16S rRNA gene sequencing revealed it to be Comamonas sp. The biodegradation of the SDM was analyzed by UV-vis spectroscopy, IR spectroscopy and GC-MS analysis. The isolate showed high metabolic activity towards SDM and degraded it completely (100 mg L(-1)) within 30 h at pH 7 and 35 °C. Simulated microcosm studies in the presence and absence of indigenous microflora confirmed the ability of Comamonas sp. VS-MH2 for dye degradation and to colonize the soil. This is the first investigation reporting the degradation of SDM by Comamonas sp. under simulated soil microcosms.
authors: Department of Microbiology, P.D. Patel Institute of Applied Sciences, Charotar University of Science & Technology, Changa, 388 421 Gujarat, India. hilor_pathak@yahoo.co.in
authors: Hilor
authors: H
authors: Pathak
authors: Sonal
authors: S
authors: Patel
authors: Meeta
authors: M
authors: Rathod
authors: Kishor
authors: K
authors: Chauhan
mesh_terms: D000704:Analysis of Variance; D001483:Base Sequence; D001673:Biodegradation, Environmental / Q000187:drug effects; D003116:Color; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D020585:Comamonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007887:Fabaceae / Q000187:drug effects; D008954:Models, Biological; D009584:Nitrogen / Q000494:pharmacology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D018675:Toxicity Tests
chemical_list: D004396:Coloring Agents; D012336:RNA, Ribosomal, 16S; D009584:Nitrogen