title: The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT.
abstract: The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila.
authors: Department of Molecular Biology and Ecology of Plants, Tel-Aviv University, Tel-Aviv, Israel.
authors: Gal
authors: G
authors: Nissan
authors: Shulamit
authors: S
authors: Manulis-Sasson
authors: Laura
authors: L
authors: Chalupowicz
authors: Doron
authors: D
authors: Teper
authors: Adva
authors: A
authors: Yeheskel
authors: Metsada
authors: M
authors: Pasmanik-Chor
authors: Guido
authors: G
authors: Sessa
authors: Isaac
authors: I
authors: Barash
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D029748:Caryophyllaceae / Q000235:genetics* / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D058507:Host Specificity; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D020636:Pantoea / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology*; D010941:Plant Tumors; D011401:Promoter Regions, Genetic / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D015854:Up-Regulation; D014774:Virulence / Q000235:genetics; D016335:Zinc Fingers
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C113709:HsvG protein, Pantoea agglomerans; D019913:Nuclear Localization Signals; D014157:Transcription Factors