title: Escherichia coli virulence genes profile of surface waters as an indicator of water quality.
abstract: We compared the presence of 58 known virulence genes (VGs) associated with Escherichia coli strains causing intestinal (InPEC) and extra-intestinal (ExPEC) infections in three estuarine, four brackish and 13 freshwater sites during the dry and wet seasons. The most common VGs observed in water samples during the dry season belonged to ExPEC (traT; 80% and ompA; 70%) whilst east1 (70%) gene was the most common among InPEC. More types of VGs were observed in water samples during wet season and included those found among InPEC (e.g. eaeA; 100%; fyuA, 90%; paa, 65%; cdt, 60%; and stx(2), 60%) and ExPEC (e.g. iroN(E.coli), 90%; iss, 90% and kpsMTII, 80%). Eight VGs were found exclusively in the wet season, of which four were found in all three water types indicating their association with storm-water run off. The number of VGs associated with ExPEC were significantly (P < 0.05) higher in only brackish and estuarine waters during the wet season compared to the dry season. There was no correlation between the number of E. coli and the presence of VGs in any of the water types in both seasons but we found similarities in VG profiles of sites with similar land uses.
authors: Faculty of Science, Health and Education, University of the Sunshine Coast, Maroochydore DC, Queensland 4558, Australia.
authors: N
authors: N
authors: Masters
authors: A
authors: A
authors: Wiegand
authors: W
authors: W
authors: Ahmed
authors: M
authors: M
authors: Katouli
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D005243:Feces / Q000382:microbiology; D005618:Fresh Water / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics*; D005843:Geography; D010802:Phylogeny; D011793:Queensland; D012621:Seasons; D014774:Virulence / Q000235:genetics; D014871:Water Microbiology; D014876:Water Pollution; D060753:Water Quality