title: HPLC-UV analytical method for determination of pizotifen after in vitro transdermal diffusion studies.
abstract: Pizotifen malate is an antihistamine and serotonin inhibitor used in the preventive treatment of migraine and eating disorders. A simple, rapid, accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection was validated for the quantitative analysis of pizotifen malate in samples from in vitro transdermal diffusion studies. The method was validated for specificity, linearity, accuracy, precision, limit of detection, limit of quantification and robustness. Drug stability in the solution was also determined under different conditions. Separation was carried out using a 250 × 4.0 mm Kromasil(®) C(18) column at room temperature. The detector response, fitted at 254 nm, was found to be linear in a concentration range between 0.24 and 24.0 µg/mL. The limit of detection was 0.02 µg/mL and the limit of quantification was 0.07 µg/mL. Finally, in vitro transdermal diffusion of pizotifen malate was characterized using the validated HPLC method.
authors: Departamento de Fisiología, Farmacología y Toxicología, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Ed. Seminario s/n, 46113, Moncada, Valencia, Spain. cesar.serna@uch.ceu.es
authors: C E
authors: CE
authors: Serna-Jiménez
authors: S
authors: S
authors: del Rio Sancho
authors: M A
authors: MA
authors: Calatayud-Pascual
authors: C
authors: C
authors: Balaguer-Fernández
authors: A
authors: A
authors: Femenía-Font
authors: A
authors: A
authors: López-Castellano
authors: V
authors: V
authors: Merino
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004058:Diffusion; D015194:Diffusion Chambers, Culture; D004355:Drug Stability; D057230:Limit of Detection; D016014:Linear Models; D010918:Pizotyline / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013552:Swine
chemical_list: D010918:Pizotyline