title: Differences in control by UV radiation of inflammatory airways disease in naïve and allergen pre-sensitised mice.
abstract: Exposure of skin to UV radiation (UVR) prior to allergen exposure can inhibit inflammatory airways disease in mice by reducing effector CD4+ T cells in both the trachea and the airway draining lymph nodes. This study analysed the immunomodulatory properties of UVR delivered to naïve versus allergen pre-sensitised mice. In a model of inflammatory airways disease, BALB/c mice were sensitised by peritoneal injection of the allergen, ovalbumin (OVA) (20 μg/mouse), in the adjuvant, alum (4 mg/mouse), on days 0 and 14. On day 21, the mice were exposed to aerosolised OVA and 24 h later, proliferative responses by the cells in the airway draining lymph nodes were examined. UVR (8 kJ m(-2)) was administered 3 days prior to first OVA sensitisation (day -3), or OVA aerosol challenge (day 18). UVR before sensitisation reduced immune responses associated with expression of allergic airways disease; seven days after first OVA sensitisation, regulation of OVA-induced proliferation in vitro but not in vivo by CD4+CD25+ cells from UV-irradiated mice was detected. UVR administered to pre-sensitised mice regulated allergen responsiveness by cells from the airway draining lymph nodes only with a sensitisation protocol involving allergen and adjuvant at 5% strength of the original dose (1 μg OVA in 0.2 mg alum/mouse). These results suggest that UVR may modulate allergic airways disease by two mechanisms. The first, and more potent, is by reducing effector cells in respiratory tissues and requires UV delivery prior to sensitisation. The second, associated with administration to pre-sensitised mice, is weaker and is detected when the mice are sensitised with lower levels of allergen and adjuvant.
authors: Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, West Perth, Australia.
authors: Naomi M
authors: NM
authors: Scott
authors: Misty J M
authors: MJ
authors: Lambert
authors: Shelley
authors: S
authors: Gorman
authors: Jacqueline P
authors: JP
authors: McGlade
authors: Prue H
authors: PH
authors: Hart
mesh_terms: D000485:Allergens / Q000276:immunology* / Q000494:pharmacology; D000534:Alum Compounds / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D007274:Injections, Intraperitoneal; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008198:Lymph Nodes / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D012130:Respiratory Hypersensitivity / Q000276:immunology*; D014466:Ultraviolet Rays
chemical_list: D000485:Allergens; D000534:Alum Compounds; D053645:Interleukin-2 Receptor alpha Subunit; D010047:Ovalbumin