title: Phase II trial: undifferentiated versus differentiated autologous mesenchymal stem cells transplantation in Egyptian patients with HCV induced liver cirrhosis.
abstract: The study was aimed to evaluate the effect of autologous transplantation of BM-derived undifferentiated and differentiated MSCs in cirrhotic patients following chronic hepatitis C virus infection. Twenty-five patients with Child C liver cirrhosis, MELD score >12 were included. They were divided into 2 groups. Group I, the MSCs group (n=15), this group was subdivided into two subgroups: Ia & Ib (undifferentiated and differentiated respectively). Group II (control group; n=10) involved patients with cirrhotic liver under conventional supportive treatment. Ninety ml BM was aspirated from the iliac bone for separation of MSCs. Surface expression of CD271, CD29 and CD34 were analyzed using flowcytometry. Hepatogenesis was assessed by immunohistochemical expression of OV6, AFP and albumin. Finally approximately 1 million MSCs/Kg were suspended in saline and were placed in blood bag and injected slowly intravenously over 15 min at a rate of 5 drops/min in one session. Follow up of patients at 3 and 6 months postinfusion revealed partial improvement of liver function tests with elevation of prothrombin concentration and serum albumin levels, decline of elevated bilirubin and MELD score in MSCs group. Statistical comparisons between the two subgroups (group Ia & Ib) did not merit any significant difference regarding clinical and laboratory findings.
  
  
  CONCLUSIONS
  Bone marrow MSCs transplantation either undifferentiated or differentiated can be used as a potential treatment for liver cirrhosis.
authors: Department of Clinical Pathology, Kasr El-Aini, Cairo University, Cairo, Egypt.
authors: Mervat
authors: M
authors: El-Ansary
authors: Iman
authors: I
authors: Abdel-Aziz
authors: Sherif
authors: S
authors: Mogawer
authors: Samah
authors: S
authors: Abdel-Hamid
authors: Olfat
authors: O
authors: Hammam
authors: Salwa
authors: S
authors: Teaema
authors: Marwa
authors: M
authors: Wahdan
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D004534:Egypt; D005260:Female; D016174:Hepacivirus; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000821:virology; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D008103:Liver Cirrhosis / Q000628:therapy* / Q000821:virology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D019012:Integrin beta1; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor