title: Inhibition of p42 MAPK using a nonviral vector-delivered siRNA potentiates the anti-tumor effect of metformin in prostate cancer cells.
abstract: OBJECTIVE
  The aim of this work was to study if a G1-polyamidoamine dendrimer/siRNA dendriplex can remove the p42 MAPK protein in prostate cancer cells and to potentiate the anti-tumoral effect of the antidiabetic drug metformin and taxane docetaxel.
  
  
  METHODS
  The dendriplex uptake was studied using flow cytometry analysis. Transfection efficiency was determined by measuring p42 MAPK mRNA and protein levels. Anti-tumoral effects were determined by measuring cellular proliferation and damage.
  
  
  RESULTS
  The dendriplex siRNA/G1-polyamidoamine dendrimer decreased both p42 MAPK mRNA and protein levels by more than 80%, which potentiates the anti-tumoral effects of metformin.
  
  
  CONCLUSIONS
  Blockade of the MAPK pathway using a dendrimer-vehiculized siRNA to block the MAPK signaling pathway in prostate cancer cells can potentiate the anti-tumoral activity of anticancer drugs, indicating that the combination of siRNA-mediated blockade of survival signals plus anti-tumoral therapy might be a useful approach for cancer therapy.
authors: NanoDrugs, S.L. Parque Científico y Tecnológico, Albacete, Spain.
authors: Silvia
authors: S
authors: Monteagudo
authors: Francisco C
authors: FC
authors: Pérez-Martínez
authors: María D
authors: MD
authors: Pérez-Carrión
authors: Javier
authors: J
authors: Guerra
authors: Sonia
authors: S
authors: Merino
authors: María Prado
authors: MP
authors: Sánchez-Verdú
authors: Valentín
authors: V
authors: Ceña
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D034741:RNA, Small Interfering; D008687:Metformin; D019950:Mitogen-Activated Protein Kinase 1; D053148:Caspase 3; D006836:Hydro-Lyases; C045581:lactate dehydratase