title: The protective role of laparoscopic antireflux surgery against aspiration of pepsin after lung transplantation.
abstract: BACKGROUND
  The goal of this study was to determine, in lung transplant patients, if laparoscopic antireflux surgery (LARS) is an effective means to prevent aspiration as defined by the presence of pepsin in the bronchoalveolar lavage fluid (BALF).
  
  
  METHODS
  Between September 2009 and November 2010, we collected BALF from 64 lung transplant patients at multiple routine surveillance assessments for acute cellular rejection, or when clinically indicated for diagnostic purposes. The BALF was tested for pepsin by enzyme-linked immunosorbent assay (ELISA). We then compared pepsin concentrations in the BALF of healthy controls (n = 11) and lung transplant patients with and without gastroesophageal reflux disease (GERD) on pH-monitoring (n = 8 and n = 12, respectively), and after treatment of GERD by LARS (n = 19). Time to the development of bronchiolitis obliterans syndrome was contrasted between groups based on GERD status or the presence of pepsin in the BALF.
  
  
  RESULTS
  We found that lung transplant patients with GERD had more pepsin in their BALF than lung transplant patients who underwent LARS (P = .029), and that pepsin was undetectable in the BALF of controls. Moreover, those with more pepsin had quicker progression to BOS and more acute rejection episodes.
  
  
  CONCLUSIONS
  This study compared pepsin in the BALF from lung transplant patients with and without LARS. Our data show that: (1) the detection of pepsin in the BALF proves aspiration because it is not present in healthy volunteers, and (2) LARS appears effective as a measure to prevent the aspiration of gastroesophageal refluxate in the lung transplant population. We believe that these findings provide a mechanism for those studies suggesting that LARS may prevent nonallogenic injury to the transplanted lungs from aspiration of gastroesophageal contents.
authors: Department of Surgery, Loyola University Medical Center, Stritch School of Medicine, Maywood, IL 60153, USA. pfisichella@lumc.edu
authors: P Marco
authors: PM
authors: Fisichella
authors: Christopher S
authors: CS
authors: Davis
authors: Peter W
authors: PW
authors: Lundberg
authors: Erin
authors: E
authors: Lowery
authors: Ellen L
authors: EL
authors: Burnham
authors: Charles G
authors: CG
authors: Alex
authors: Luis
authors: L
authors: Ramirez
authors: Karen
authors: K
authors: Pelletiere
authors: Robert B
authors: RB
authors: Love
authors: Paul C
authors: PC
authors: Kuo
authors: Elizabeth J
authors: EJ
authors: Kovacs
mesh_terms: D000328:Adult; D001989:Bronchiolitis Obliterans / Q000209:etiology / Q000517:prevention & control; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016099:Endoscopy, Gastrointestinal; D005260:Female; D005764:Gastroesophageal Reflux / Q000601:surgery*; D006801:Humans; D010535:Laparoscopy; D016040:Lung Transplantation / Q000009:adverse effects / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010434:Pepsin A / Q000378:metabolism*; D011446:Prospective Studies; D053120:Respiratory Aspiration / Q000209:etiology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D010434:Pepsin A