title: Biodistribution of 177Lu-octreotate and 111In-minigastrin in female             nude mice transplanted with human medullary thyroid carcinoma GOT2.
abstract: To be able to evaluate new radiopharmaceuticals and optimize diagnostic             and therapeutic procedures, relevant animal models are required. The aim of this             study was to evaluate the medullary thyroid carcinoma GOT2 animal model by analyzing             the biodistribution of 177Lu-octreotate and 111In-minigastrin (MG0). BALB/c nude             mice, subcutaneously transplanted with GOT2, were intravenously injected with             either 177Lu-octreotate or 111In-MG0, with or without excess of unlabeled human             minigastrin simultaneously with 111In-MG0. Animals were sacrificed 1-7 days after             injection in the 177Lu-octreotate study and 1 h after injection of 111In-MG0.             The activity concentrations in organs and tissues were determined and mean absorbed             doses from 177Lu were calculated. There was a specific tumor uptake of either             177Lu-octreotate or 111In-MG0. 177Lu-octreotate samples showed high activity concentrations             in tissues expressing somatostatin receptors (SSTR). For both radiopharmaceuticals             the highest activity concentrations were found in the kidneys. Compared to results             from similar studies in mice with another MTC cell line (TT) the biodistribution             was favorable (higher tumor uptake) for the GOT2 model, while compared to other             animal models expressing SSTR, the tumor uptake of 177Lu-octreotate was modest.             In conclusion, the GOT2 animal model is a valuable model for evaluation and optimization             of diagnostic and therapeutic procedures using radiolabeled somatostatin, CCK2             and gastrin analogues prior to clinical studies.
authors: Department of Radiation Physics, University of Gothenburg, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden. johanna.dalmo@radfys.gu.se
authors: Johanna
authors: J
authors: Dalmo
authors: Nils
authors: N
authors: Rudqvist
authors: Johan
authors: J
authors: Spetz
authors: Peter
authors: P
authors: Laverman
authors: Ola
authors: O
authors: Nilsson
authors: Håkan
authors: H
authors: Ahlman
authors: Eva
authors: E
authors: Forssell-Aronsson
mesh_terms: D000818:Animals; D018278:Carcinoma, Neuroendocrine; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005755:Gastrins / Q000493:pharmacokinetics*; D006801:Humans; D007205:Indium Radioisotopes / Q000493:pharmacokinetics*; D051379:Mice; D008819:Mice, Nude; D015282:Octreotide / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: C554548:177Lu-octreotate; D005755:Gastrins; D007205:Indium Radioisotopes; D019275:Radiopharmaceuticals; C008530:minigastrin; D015282:Octreotide