title: Stable protein expression in mammalian cells using baculoviruses.
abstract: The baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) has been widely used in biotechnology for protein expression in insect cells. Baculoviruses use arthropods as their natural hosts and are unable to replicate in mammalian cells. However, AcMNPV is able to enter many mammalian cell types and can be used for transgene expression if engineered to contain suitable expression cassettes. In this chapter, we describe the construction and application of a recombinant baculovirus containing a bicistronic expression cassette that can be used for stable protein expression in mammalian cells. As an example, the generation of glioblastoma and hepatocellular carcinoma cell lines stably expressing green fluorescent protein after puromycin selection is shown.
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Andreas
authors: A
authors: Lackner
authors: Emanuel
authors: E
authors: Kreidl
authors: Barbara
authors: B
authors: Peter-Vörösmarty
authors: Sabine
authors: S
authors: Spiegl-Kreinecker
authors: Walter
authors: W
authors: Berger
authors: Michael
authors: M
authors: Grusch
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000254:growth & development; D002417:Cattle; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*; D014161:Transduction, Genetic; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D004247:DNA