title: Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O28.
abstract: An O-polysaccharide and oligosaccharides were isolated by GPC following mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O28. The O-polysaccharide was studied by sugar and methylation analyses, (1)H and (13)C NMR spectroscopy, including 2D ROESY and H-detected (1)H,(13)C HSQC and HMBC experiments, and the following structure of the branched pentasaccharide repeating unit was established: [see formula in text]. This structure was confirmed by ESI MS of the isolated tridecasaccharide consisting of the lipopolysaccharide core and one O-polysaccharide repeat. The ESI mass spectrum also enabled inferring the composition of the core oligosaccharide.
authors: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. olga.ovchinnikova@gmail.com
authors: Olga G
authors: OG
authors: Ovchinnikova
authors: Nina A
authors: NA
authors: Kocharova
authors: Anna N
authors: AN
authors: Kondakova
authors: Magdalena
authors: M
authors: Bialczak-Kokot
authors: Alexander S
authors: AS
authors: Shashkov
authors: Yuriy A
authors: YA
authors: Knirel
authors: Antoni
authors: A
authors: Rozalski
mesh_terms: D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens