title: Serum butyrylcholinesterase and paraoxonase 1 in a canine model of endotoxemia: effects of choline administration.
abstract: Butyrylcholinesterase (BChE) and paraoxonase 1 (PON1) are two serum enzymes synthesized by the liver that are related with inflammation. The main objectives of this study were to determine changes in serum BChE and PON1 by using a canine model of endotoxemia, and to evaluate whether choline alters BChE and PON1 activities during inflammation. For this purpose, a total of 20 mongrel dogs were divided into four groups: control, choline (C), lipopolysaccharide (LPS), and LPS+C. Dogs in the control group were injected with 0.9% NaCl (0.2 ml/kg, i.v.). Dogs in C and LPS+C groups received choline chloride (20 mg/kg, i.v., three times with 4 h intervals). Endotoxin was injected (0.02 mg/kg, i.v., once) to the dogs of LPS and LPS+C groups. Statistically significant decreases in BChE and PON1 activities in LPS group were detected 24 and 48 h post injection, respectively. No statistically significant changes in BChE and PON1 activities at different times were detected in control, C, or LPS+C groups. In conclusion, the data obtained in present study revealed a decrease in serum BChE and PON1 activities in dogs during experimentally induced endotoxemia and that choline administration attenuates these changes.
authors: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Murcia University, 30100 Murcia, Spain.
authors: Asta
authors: A
authors: Tvarijonaviciute
authors: Meric
authors: M
authors: Kocaturk
authors: Mehmet
authors: M
authors: Cansev
authors: Fernando
authors: F
authors: Tecles
authors: Jose J
authors: JJ
authors: Ceron
authors: Zeki
authors: Z
authors: Yilmaz
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D001831:Body Temperature / Q000187:drug effects; D002091:Butyrylcholinesterase / Q000378:metabolism*; D002794:Choline / Q000494:pharmacology*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism / Q000662:veterinary*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D012119:Respiration / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D002091:Butyrylcholinesterase; D043303:Aryldialkylphosphatase; D002794:Choline