title: Immune interference in Mycobacterium tuberculosis intracellular iron acquisition through siderocalin recognition of carboxymycobactins.
abstract: The innate immune system antibacterial protein Siderocalin (Scn) binds ferric carboxymycobactin (CMB) and also several catecholate siderophores. Although the recognition of catecholates by Scn has been thoroughly investigated, the binding interactions of Scn with the full spectrum of CMB isoforms have not been studied. Here we show that Scn uses different binding modes for the limited subset of bound CMB isoforms, resulting in a range of binding affinities that are much weaker than other siderophore targets of Scn. Understanding the binding interaction between Scn and CMBs provides clues for the influence of Scn on mycobacterial iron acquisition.
authors: Trisha M
authors: TM
authors: Hoette
authors: Matthew C
authors: MC
authors: Clifton
authors: Anna M
authors: AM
authors: Zawadzka
authors: Meg A
authors: MA
authors: Holmes
authors: Roland K
authors: RK
authors: Strong
authors: Kenneth N
authors: KN
authors: Raymond
mesh_terms: D000209:Acute-Phase Proteins / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000502:physiology*; D008958:Models, Molecular; D008968:Molecular Conformation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000378:metabolism*; D010080:Oxazoles / Q000737:chemistry / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D055672:Static Electricity
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D007502:Iron Chelating Agents; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D010080:Oxazoles; D011518:Proto-Oncogene Proteins; C018608:mycobactins; D007501:Iron