title: Effects of zao huang mixture (see text) on the expressions of TGF-beta1 and Col IV in human glomerular mesangial cells cultured in high glucose environment.
abstract: OBJECTIVE
  To investigate the effect of Zao Huang Mixture (see text) on expressions of growth factor-beta1 (TGF-beta1) and collagen IV (Col IV) in human glomerular mesangial cells (GMC) cultured in high-glucose environment.
  
  
  METHODS
  After primary culture of GMC, in vitro culture was carried out in normal group, high glucose group and high glucose medium with ZHM of different concentrations, and the expressions of TGF-beta1 and Col IV in the GMC group and in ZHM group were detected at 24 and 48 h respectively.
  
  
  RESULTS
  Compared with the normal group, expressions of TGF-beta1 and Col IV significantly increased at 24 h, 48 h in the high glucose group (all P < 0.01); Compared with the high glucose group, the expressions of TGF-beta1 and Col IV in all the ZHM groups significantly decreased at 24 h, 48 h (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  ZHM may modulate the process of diabetic nephropathy by changing the expression of TGF-beta1 and Col IV in glomerular mesangial cells.
authors: Yantai Economy and Technology Development Zone Hospital, Yantai 264006, China. jujianwei1234@sina.com
authors: Jian-Wei
authors: JW
authors: Ju
authors: Shou-Han
authors: SH
authors: Mu
authors: Yan-Zhi
authors: YZ
authors: Yin
authors: Cong-Kui
authors: CK
authors: Lü
authors: Xiao-Yan
authors: XY
authors: Sun
authors: Lei-Xiu
authors: LX
authors: Deng
mesh_terms: D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000188:drug therapy*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008516:Medicine, Chinese Traditional; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D024141:Collagen Type IV; D053773:Transforming Growth Factor beta1; D005947:Glucose