title: Evaluation of antioxidant and antiulcerogenic activities of Opuntia ficus indica f. inermis flowers extract in rats.
abstract: The Opuntia ficus indica f. inermis methanolic flowers extract (OMFE) was phytochemical studied, in vitro tested for their potential antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, linoleic acid peroxidation assays and in vivo evaluated for its ability to prevent ethanol-induced gastric ulcer in rats. The OMFE was rich in polysaccharide, phenolics and flavonoids contents and exhibited a moderate in vitro antioxidant activity when compared with (+)-catechin and ascorbic acid. Pre-treatment with OMFE at oral doses 250, 500 and 1000 mg/kg body weight was found to provide a dose-dependent protection against ethanol-induced gastric ulcer by averting the deep necrotic lesions of the gastric epithelium, by preserving normal antioxidant enzymes activities, by inhibiting the lipid peroxidation, the oxidation of protein and the DNA fragmentation in gastric mucosa. The antiulcerogenic activity of OMFE might be due to a possible synergistic antioxidant and antihistaminic-like effects.
authors: Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences de Gafsa, Cité Zarroug, 2112 Gafsa, Tunisia. alimihichem@yahoo.fr
authors: Hichem
authors: H
authors: Alimi
authors: Najla
authors: N
authors: Hfaiedh
authors: Zouhour
authors: Z
authors: Bouoni
authors: Mohsen
authors: M
authors: Sakly
authors: Khémais
authors: K
authors: Ben Rhouma
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004798:Enzymes / Q000378:metabolism; D000431:Ethanol / Q000009:adverse effects; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D029747:Opuntia / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D023382:Toxicity Tests, Acute
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D004798:Enzymes; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D000431:Ethanol; D019787:Linoleic Acid