title: Preventing and troubleshooting artefacts in saturation labelled fluorescence 2-D difference gel electrophoresis (saturation DiGE).
abstract: Saturation DiGE is a powerful but challenging technique for the characterisation of changes in protein expression between two or more scarce samples. In this paper, measures to prevent and troubleshoot artefacts in the saturation DiGE workflow are discussed, with illustration of some examples as they may be encountered in gel images or analysis.
authors: Centre for Cell Engineering, Division of Biochemistry and Cell Biology, Joseph Black Building, University of Glasgow, Glasgow, UK. laura.mcnamara@glasgow.ac.uk
authors: Laura E
authors: LE
authors: McNamara
authors: Fahsai A
authors: FA
authors: Kantawong
authors: Matthew J
authors: MJ
authors: Dalby
authors: Mathis O
authors: MO
authors: Riehle
authors: Richard
authors: R
authors: Burchmore
mesh_terms: D016477:Artifacts; D005456:Fluorescent Dyes; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins