title: Directed evolution combined with rational design increases activity of GpdQ toward a non-physiological substrate and alters the oligomeric structure of the enzyme.
abstract: Directed evolution was used to enhance the activity of the glycerophosphodiesterase enzyme from Enterobacter aerogenes, GpdQ, toward bis(para-nitrophenol) phosphate (BpNPP), a substrate that is frequently used to assay phosphodiesterases. Native GpdQ has a low level of activity toward BpNPP while the evolved enzymes exhibited k(cat) values that were well over 100 times better while improvements in k(cat)/K(m) of around 500 times were observed along with improved activity we observed a change in the oligomeric structure in the evolved enzymes. The native enzyme is a hexamer with tightly associated dimers related by a 3-fold axis. The stability of the dimer was attributed in part to the cap domain that forms a disulfide bond with its 2-fold-related subunit and in part due to the fact that dimerization results in burying 23.6% of the monomer's accessible surface area. The cap domain also forms the top of the active site and contributes an essential part of the interface between 3-fold-related molecules. The evolved proteins quickly lost one of the cysteine residues that formed the disulfide bond and other mutations that might stabilize the cap domain. The likely effect of these mutations was to open up the active site for the new substrate and to favor the formation of dimeric molecules. The breakdown of the oligomeric structure was accompanied by a reduction in the thermal stability of the protein-as monitored by the residual activity of the native and mutant proteins following pre-incubation at elevated temperatures. A discussion on the evolutionary implications of these studies is presented.
authors: Research School of Chemistry, Australian National University, Canberra,  Australia.
authors: Sylvia H-C
authors: SH
authors: Yip
authors: Jee-Loon
authors: JL
authors: Foo
authors: Gerhard
authors: G
authors: Schenk
authors: Lawrence R
authors: LR
authors: Gahan
authors: Paul D
authors: PD
authors: Carr
authors: David L
authors: DL
authors: Ollis
mesh_terms: D020134:Catalytic Domain; D002850:Chromatography, Gel; D019020:Directed Molecular Evolution; D021902:Enterobacter aerogenes / Q000201:enzymology; D006358:Hot Temperature; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009596:Nitrophenols / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055503:Protein Multimerization; D013379:Substrate Specificity
chemical_list: D009596:Nitrophenols; C002887:bis(4-nitrophenyl)phosphate; D010727:Phosphoric Diester Hydrolases; C038133:glycerophosphodiester phosphodiesterase