title: Effect of substituent of terpyridines on the DNA-interaction of polypyridyl ruthenium(II) complexes.
abstract: An octahedral complexes of ruthenium with 2,9-dimethyl-1,10-phenanthroline (dmphen) and substituted terpyridine have been synthesized. The Ru(II) complexes have been characterized by elemental analyses, thermogravimetric analyses, magnetic moment measurements, FT-IR, electronic, (1)H NMR and FAB mass spectra. The binding strength and mode of interaction of the complexes with Herring Sperm DNA has been investigated using absorption titration and viscosity measurement studies. Results suggest that the substituent on terpyridine ligand affects the binding mode and binding ability of the complexes. Effect of time and ionic strength on DNA cleavage ability of complex has also been studied by gel electrophoresis. Results suggest that more than 200 mM concentration of NaCl decreases the cleavage ability of complex.
authors: Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India. jeenen@gmail.com
authors: Mohan N
authors: MN
authors: Patel
authors: Deepen S
authors: DS
authors: Gandhi
authors: Pradhuman A
authors: PA
authors: Parmar
mesh_terms: D000042:Absorption; D000818:Animals; D004247:DNA / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D060328:Magnetic Phenomena; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013094:Spermatozoa / Q000378:metabolism; D013818:Thermogravimetry; D013997:Time Factors; D014783:Viscosity
chemical_list: D004270:DNA, Circular; D004278:DNA, Superhelical; D009942:Organometallic Compounds; D011725:Pyridines; D012428:Ruthenium; D004247:DNA