title: Salinisphaera orenii sp. nov., isolated from a solar saltern.
abstract: A taxonomic study was performed on two isolates, designated strains MK-B5(T) and MK-B7, isolated from sediment of a solar saltern pond in Gomso Bay, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strains MK-B5(T) and MK-B7 belong to the Gammaproteobacteria and are related most closely to Salinisphaera shabanensis JCM 11575(T) ( = E1L3A(T)) (96.3 and 96.5% similarity, respectively), Salinisphaera dokdonensis KCCM 90064(T) ( = CL-ES53(T)) (95.6 and 95.6%) and Salinisphaera hydrothermalis JCM 115514(T) ( = EPR70(T)) (95.1 and 95.3%). The level of 16S rRNA gene sequence similarity between strains MK-B5(T) and MK-B7 was 99.8%. The G+C contents of their genomic DNAs were 63.4 and 63.6 mol%, respectively, and the major respiratory quinone was ubiquinone-8. DNA-DNA relatedness between strains MK-B5(T) and MK-B7 was 98%, indicating that the two isolates represent a single species. However, the level of DNA-DNA relatedness between the two isolates and S. shabanensis E1L3A(T) (26.4-30.8%) indicates that they represent a novel species. Strains MK-B5(T) and MK-B7 possessed C(14:0), C(16:0) and C(19:0)ω8c cyclo as major fatty acids. The two isolates were Gram-stain-negative, strictly aerobic, short rod-shaped and motile. They grew at 10-40 °C (optimum, 35-37 °C), at pH 5.0-8.5 (optimum, 7.0-7.5) and with 5-25% (w/v) NaCl (optimum, 15% NaCl). On the basis of phenotypic and phylogenetic analyses, strains MK-B5(T) and MK-B7 are thus considered to represent a novel species of the genus Salinisphaera, for which the name Salinisphaera orenii sp. nov. is proposed. The type strain is MK-B5(T) ( = KCTC 23198(T) = JCM 17073(T)).
authors: Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: Soo-Je
authors: SJ
authors: Park
authors: Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: In-Tae
authors: IT
authors: Cha
authors: Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: So-Jeong
authors: SJ
authors: Kim
authors: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors: Kee-Sun
authors: KS
authors: Shin
authors: Department of Biochemistry, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: YoungSoo
authors: Y
authors: Hong
authors: Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: Dong-Hyun
authors: DH
authors: Roh
authors: Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.
authors: Sung-Keun
authors: SK
authors: Rhee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone