title: [Genetic evolution of VP1 of enterovirus type 71 in Shenzhen].
abstract: OBJECTIVE
  Genetic evolution of VP1 of enterovirus type 71 in Shenzhen were analyzed.
  
  
  METHODS
  All samples were tested by RT-PCR using EV71 specific primer. The VP1 of EV71 were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with subgenotype A, B and C using DNAStar, BioEdit and Mega 3.1 software.
  
  
  RESULTS
  Among 35 strains, the homogeneity of the VP1 nucleotide sequence was between 92.1%-100%. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was between 81.4% -91.1%. The VP1 nucleotide sequence of 35 strains of Shenzhen shared between 93% -97.4% identity with cluster C4. The prevalence strains of EV71 were cluster C4b from 1998 to 2004, and gradually moved to C4a since 2003. All of EV71 were C4b from 2006 to 2008. Also, the homogeneity of the VP1 nucleotide sequence with Anhui FY23 EV71 strain were 94.5% -94.7%, 95.7% -95.8%, 96.2%, 95.4% -97.5%, 96.3% -99.2% from 2003 to 2008. It shows that the homogeneity was increased year by year. There was a mutation (A --> C) at No. 66 nucleotide of VP1 of EV71 that two strains were isolated in 2003 and 8 strains in 2008, that caused amino acid mutation (Q --> H) at No. 22 of VP1.
  
  
  CONCLUSIONS
  EV71 C4b was gradually moved to C4a from 1998 to 2008. There was a missense mutation at No. 66 nucleotide of VP1.
authors: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. heyaqing1019@126.com
authors: Ya-Qing
authors: YQ
authors: He
authors: Hong-Bin
authors: HB
authors: Zhang
authors: Xiang-Jie
authors: XJ
authors: Yao
authors: Yu-Xue
authors: YX
authors: Liao
authors: Hong
authors: H
authors: Yang
authors: Hui-Xia
authors: HX
authors: Xian
authors: Fan
authors: F
authors: Yang
authors: Hai-Long
authors: HL
authors: Zhang
authors: Xiao-Ke
authors: XK
authors: Yang
authors: Wen-Bo
authors: WB
authors: Xu
mesh_terms: D004770:Enterovirus / Q000145:classification / Q000235:genetics*; D006801:Humans; D020125:Mutation, Missense / Q000235:genetics; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015678:Viral Structural Proteins / Q000145:classification / Q000235:genetics
chemical_list: D015678:Viral Structural Proteins