title: Triamcinolone up-regulates GLUT 1 and GLUT 3 expression in cultured human placental endothelial cells.
abstract: The placenta is a glucocorticoid target organ, and glucocorticoids (GCs) are essential for the development and maturation of fetal organs. They are widely used for treatment of a variety of diseases during pregnancy. In various tissues, GCs have regulated by glucose transport systems; however, their effects on glucose transporters in the human placental endothelial cells (HPECs) are unknown. In the present study, HPECs were cultured 24 h in the presence or absence of 0.5, 5 and 50 µmol · l(-1) of synthetic GC triamcinolone (TA). The glucose carrier proteins GLUT 1, GLUT 3 and GC receptor (GR) were detected in the HPECs. We showed increased expression of GLUT 1 and GLUT 3 proteins and messenger RNA (mRNA) levels (p < 0.05) after 24-h cell culture in the presence of 0.5, 5 and 50 µmol · l(-1) of TA. In contrast, GR protein and mRNA expressions were down-regulated (p < 0.05) with 0.5, 5 and 50 µmol · l(-1) of TA 24-h cell culture. The results demonstrate that GCs are potent regulators of placental GLUT 1 and GLUT 3 expression through GR. Excessive exposure to GCs causes maternal and fetal hypoglycemia and diminished fetal growth. We speculate that to compensate for fetal hypoglycemia and diminished fetal growth, the expression of placental endothelial glucose transporters might be increased.
authors: Department of Biochemistry, Medical Faculty, Akdeniz University, Antalya, Turkey.
authors: Dijle
authors: D
authors: Kipmen-Korgun
authors: Asli
authors: A
authors: Ozmen
authors: Gozde
authors: G
authors: Unek
authors: Mehmet
authors: M
authors: Simsek
authors: Ramazan
authors: R
authors: Demir
authors: Emin Turkay
authors: ET
authors: Korgun
mesh_terms: D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D006801:Humans; D010920:Placenta / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D014221:Triamcinolone / Q000494:pharmacology*
chemical_list: D005938:Glucocorticoids; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D011965:Receptors, Glucocorticoid; C495677:SLC2A1 protein, human; D014221:Triamcinolone