title: GRB7 is required for triple-negative breast cancer cell invasion and survival.
abstract: Triple-negative breast cancer (TNBC) is a heterogeneous disease that is usually associated with poor prognosis, and frequently associated with the basal-like breast cancer gene expression profile. There are no targeted therapeutic modalities for this disease, and no useful biomarkers. High GRB7 RNA expression levels are associated with an elevated risk of recurrence in patients with operable TNBC treated with standard adjuvant anthracycline and taxane therapy. To determine whether GRB7 is involved in the pathobiology of TNBC, we evaluated the biological effects of GRB7 inhibition in a panel of triple-negative cell lines-MDA-MB-468, MDA-MB-231, HCC70, and T4-2. We found GRB7 inhibition reduced cell motility and invasion of these cell lines and promoted cell death by apoptosis in 3D culture. These data suggest that GRB7 itself, or GRB7-dependent pathways, may prove to be important therapeutic targets in this disease.
authors: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors: Orsi
authors: O
authors: Giricz
authors: Verónica
authors: V
authors: Calvo
authors: Stephanie C
authors: SC
authors: Pero
authors: David N
authors: DN
authors: Krag
authors: Joseph A
authors: JA
authors: Sparano
authors: Paraic A
authors: PA
authors: Kenny
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005260:Female; D051382:GRB7 Adaptor Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics; D018295:Neoplasms, Basal Cell / Q000235:genetics / Q000473:pathology; D010456:Peptides, Cyclic / Q000494:pharmacology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000172:deficiency; D011960:Receptors, Estrogen / Q000172:deficiency; D011980:Receptors, Progesterone / Q000172:deficiency; D015203:Reproducibility of Results
chemical_list: D051382:GRB7 Adaptor Protein; D010456:Peptides, Cyclic; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C509376:G7-18NATE