title: Cohnella cellulosilytica sp. nov., isolated from buffalo faeces.
abstract: A cellulose-degrading bacterium, strain FCN3-3(T), was isolated from buffalo faeces collected in Nakhonnayok province, Thailand. The strain was characterized based on its phenotypic and genotypic characteristics. Strain FCN3-3(T) was a Gram-positive, aerobic, spore-forming, rod-shaped bacterium. It contained meso-diaminopimelic acid in cell-wall peptidoglycan. The major menaquinone was MK-7. Anteiso-C(15:0) (52.5%), iso-C(16:0) (18.9%) and C(16:0) (9.1%) were the predominant cellular fatty acids, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major phospholipids. The DNA G+C content was 58.0 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain FCN3-3(T) was affiliated to the genus Cohnella and was closely related to Cohnella phaseoli GSPC1(T), Cohnella luojiensis HY-22R(T) and Cohnella hongkongensis HKU3(T), with 97.2, 96.8 and 96.3% sequence similarity, respectively. Strain FCN3-3(T) could be clearly distinguished from all known species of the genus Cohnella by its physiological and biochemical characteristics as well as its phylogenetic position and level of DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Cohnella, for which the name Cohnella cellulosilytica sp. nov. is proposed; the type strain is FCN3-3(T) ( = KCTC 13645(T) = TISTR 1996(T) = PCU 323(T)).
authors: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Saowapar
authors: S
authors: Khianngam
authors: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Somboon
authors: S
authors: Tanasupawat
authors: Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Ancharida
authors: A
authors: Akaracharanya
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors: Kwang Kyu
authors: KK
authors: Kim
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors: Keun Chul
authors: KC
authors: Lee
authors: Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors: Jung-Sook
authors: JS
authors: Lee
mesh_terms: D000818:Animals; D001482:Base Composition; D002020:Buffaloes / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S