title: Magnesium sulfate enhances non-depolarizing muscle relaxant vecuronium action at adult muscle-type nicotinic acetylcholine receptor in vitro.
abstract: OBJECTIVE
  To investigate the effect of magnesium sulfate and its interaction with the non-depolarizing muscle relaxant vecuronium at adult muscle-type acetylcholine receptors in vitro.
  
  
  METHODS
  Adult muscle-type acetylcholine receptors were expressed in HEK293 cells. Drug-containing solution was applied via a gravity-driven perfusion system. The inward currents were activated by brief application of acetylcholine (ACh), and recorded using whole-cell voltage-clamp technique.
  
  
  RESULTS
  Magnesium sulfate (1-100 mmol/L) inhibited the inward currents induced ACh (10 μmol/L) in a concentration-dependent manner (IC(50)=29.2 mmol/L). The inhibition of magnesium sulfate was non-competitive. In contrast, vecuronium produced a potent inhibition on the adult muscle-type acetylcholine receptor (IC(50)=8.7 nmol/L) by competitive antagonism. Magnesium sulfate at the concentrations of 1, 3, and 6 mmol/L markedly enhanced the inhibition of vecuronium (10 nmol/L) on adult muscle-type acetylcholine receptors.
  
  
  CONCLUSIONS
  Clinical enhancement of vecuronium-induced muscle relaxation by magnesium sulfate can be attributed partly to synergism between magnesium sulfate and non-depolarizing muscle relaxants at adult muscle-type acetylcholine receptors.
authors: Department of Anesthesiology, the First Affiliated Hospital of Bengbu Medical College, China.
authors: Hong
authors: H
authors: Wang
authors: Qi-sheng
authors: QS
authors: Liang
authors: Lan-ren
authors: LR
authors: Cheng
authors: Xiao-hong
authors: XH
authors: Li
authors: Wei
authors: W
authors: Fu
authors: Wen-tao
authors: WT
authors: Dai
authors: Shi-tong
authors: ST
authors: Li
mesh_terms: D002460:Cell Line; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D008278:Magnesium Sulfate / Q000494:pharmacology*; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology*; D018733:Nicotinic Antagonists / Q000494:pharmacology*; D014673:Vecuronium Bromide / Q000494:pharmacology*
chemical_list: D003473:Neuromuscular Nondepolarizing Agents; D018733:Nicotinic Antagonists; D008278:Magnesium Sulfate; D014673:Vecuronium Bromide