title: Selective up-regulation of the soluble pattern-recognition receptor pentraxin 3 and of vascular endothelial growth factor in giant cell arteritis: relevance for recent optic nerve ischemia.
abstract: To assess local expression and plasma levels of pentraxin 3 (PTX3) in patients with giant cell arteritis (GCA).
  
  
  
  Plasma and serum samples were obtained from 75 patients with GCA (20 of whom had experienced optic nerve ischemia in the previous 3 weeks and 24 of whom had experienced symptom onset in the previous 6 months and had no history of optic nerve ischemia) and 63 controls (35 age-matched healthy subjects, 15 patients with rheumatoid arthritis, and 13 patients with chronic stable angina). In 9 patients in whom GCA was recently diagnosed, circulating levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12p70, CCL2/monocyte chemotactic protein 1, CCL3/macrophage inflammatory protein 1α (MIP-1α), CCL4/MIP-1β, CCL11/eotaxin, CXCL9/monokine induced by interferon-γ, CXCL10/interferon-γ-inducible 10-kd protein, tumor necrosis factor α (TNFα), interferon-γ, vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor, and FasL were measured via a multiplexed cytometric assay. PTX3 and VEGF concentrations were assessed by enzyme-linked immunosorbent assay. PTX3 and CD68 expression were determined by immunohistochemistry and immunofluorescence on temporal artery samples.
  
  
  
  GCA patients with very recent optic nerve ischemia had significantly higher PTX3 and VEGF levels compared to other GCA patients and controls. GCA patients with a disease duration of <6 months had significantly higher PTX3 levels compared to other GCA patients and controls. Immunohistochemistry revealed selective PTX3 expression in the wall of inflamed arteries.
  
  
  
  Our findings indicate that local expression of PTX3 is a feature of vascular inflammation in GCA; elevated circulating levels of PTX3 identify patients with very recent optic nerve ischemia or a recent diagnosis. Optic nerve ischemia is also associated with increased circulating VEGF levels.
authors: Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele, Milan, Italy.
authors: Mattia
authors: M
authors: Baldini
authors: Norma
authors: N
authors: Maugeri
authors: Giuseppe A
authors: GA
authors: Ramirez
authors: Chiara
authors: C
authors: Giacomassi
authors: Alessandra
authors: A
authors: Castiglioni
authors: Sergio
authors: S
authors: Prieto-González
authors: Marc
authors: M
authors: Corbera-Bellalta
authors: Gabriele
authors: G
authors: Di Comite
authors: Ilenia
authors: I
authors: Papa
authors: Giacomo
authors: G
authors: Dell'antonio
authors: Enrico
authors: E
authors: Ammirati
authors: Ivan
authors: I
authors: Cuccovillo
authors: Viviana
authors: V
authors: Vecchio
authors: Alberto
authors: A
authors: Mantovani
authors: Patrizia
authors: P
authors: Rovere-Querini
authors: Maria Grazia
authors: MG
authors: Sabbadini
authors: Maria C
authors: MC
authors: Cid
authors: Angelo A
authors: AA
authors: Manfredi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002097:C-Reactive Protein / Q000032:analysis / Q000096:biosynthesis*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D013700:Giant Cell Arteritis / Q000150:complications / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018917:Optic Neuropathy, Ischemic / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism*; D000683:Serum Amyloid P-Component / Q000032:analysis / Q000096:biosynthesis*; D013699:Temporal Arteries / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000097:blood; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D016207:Cytokines; D000683:Serum Amyloid P-Component; D042461:Vascular Endothelial Growth Factor A; D000099153:Pentraxins; C467484:VEGFA protein, human