title: Blimp1 expression predicts embryonic stem cell development in vitro.
abstract: Despite recent critical insights into the pluripotent state of embryonic stem cells (ESCs), there is little agreement over the inaugural and subsequent steps leading to its generation [1-4]. Here we show that inner cell mass (ICM)-generated cells expressing Blimp1, a key transcriptional repressor of the somatic program during germ cell specification [5, 6], emerge on day 2 of blastocyst culture. Single-cell gene expression profiling indicated that many of these Blimp1-positive cells coexpress other genes typically associated with early germ cell specification. When genetically traced in vitro, these cells acquired properties normally associated with primordial germ cells. Importantly, fate-mapping experiments revealed that ESCs commonly arise from Blimp1-positive precursors; indeed, prospective sorting of such cells from ICM outgrowths increased the rate of ESC derivation more than 9-fold. Finally, using genetic ablation or distinct small molecules [7, 8], we show that epiblast cells can become ESCs without first acquiring Blimp1 positivity. Our findings suggest that the germ cell-like state is facultative for the stabilization of pluripotency in vitro. Thus, the association of Blimp1 expression with ESC development furthers understanding of how the pluripotent state of these cells is established in vitro and suggests a means to enhance the generation of new stem cell lines from blastocysts.
authors: Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA.
authors: Li-Fang
authors: LF
authors: Chu
authors: M Azim
authors: MA
authors: Surani
authors: Rudolf
authors: R
authors: Jaenisch
authors: Thomas P
authors: TP
authors: Zwaka
mesh_terms: D000818:Animals; D015415:Biomarkers; D001755:Blastocyst; D002454:Cell Differentiation; D019070:Cell Lineage; D046149:Embryo Culture Techniques; D053595:Embryonic Stem Cells / Q000502:physiology*; D050991:GATA4 Transcription Factor / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D039904:Pluripotent Stem Cells / Q000502:physiology*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D050991:GATA4 Transcription Factor; C484171:Gata4 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C513258:Prdm1 protein, mouse; D011506:Proteins; D022661:RNA, Untranslated; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1