title: Large scale mitochondrial sequencing in Mexican Americans suggests a reappraisal of Native American origins.
abstract: BACKGROUND
  The Asian origin of Native Americans is largely accepted. However uncertainties persist regarding the source population(s) within Asia, the divergence and arrival time(s) of the founder groups, the number of expansion events, and migration routes into the New World. mtDNA data, presented over the past two decades, have been used to suggest a single-migration model for which the Beringian land mass plays an important role.
  
  
  RESULTS
  In our analysis of 568 mitochondrial genomes, the coalescent age estimates of shared roots between Native American and Siberian-Asian lineages, calculated using two different mutation rates, are A4 (27.5 ± 6.8 kya/22.7 ± 7.4 kya), C1 (21.4 ± 2.7 kya/16.4 ± 1.5 kya), C4 (21.0 ± 4.6 kya/20.0 ± 6.4 kya), and D4e1 (24.1 ± 9.0 kya/17.9 ± 10.0 kya). The coalescent age estimates of pan-American haplogroups calculated using the same two mutation rates (A2:19.5 ± 1.3 kya/16.1 ± 1.5 kya, B2:20.8 ± 2.0 kya/18.1 ± 2.4 kya, C1:21.4 ± 2.7 kya/16.4 ± 1.5 kya and D1:17.2 ± 2.0 kya/14.9 ± 2.2 kya) and estimates of population expansions within America (~21-16 kya), support the pre-Clovis occupation of the New World. The phylogeography of sublineages within American haplogroups A2, B2, D1 and the C1b, C1c and C1d subhaplogroups of C1 are complex and largely specific to geographical North, Central and South America. However some sub-branches (B2b, C1b, C1c, C1d and D1f) already existed in American founder haplogroups before expansion into the America.
  
  
  CONCLUSIONS
  Our results suggest that Native American founders diverged from their Siberian-Asian progenitors sometime during the last glacial maximum (LGM) and expanded into America soon after the LGM peak (~20-16 kya). The phylogeography of haplogroup C1 suggest that this American founder haplogroup differentiated in Siberia-Asia. The situation is less clear for haplogroup B2, however haplogroups A2 and D1 may have differentiated soon after the Native American founders divergence. A moderate population bottle neck in American founder populations just before the expansion most plausibly resulted in few founder types in America. The similar estimates of the diversity indices and Bayesian skyline analysis in North America, Central America and South America suggest almost simultaneous (~ 2.0 ky from South to North America) colonization of these geographical regions with rapid population expansion differentiating into more or less regional branches across the pan-American haplogroups.
authors: Department of Genetics, Texas Biomedical Research Institute, San Antonio, 78227, USA. skumar@txbiomedgenetics.org
authors: Satish
authors: S
authors: Kumar
authors: Claire
authors: C
authors: Bellis
authors: Mark
authors: M
authors: Zlojutro
authors: Phillip E
authors: PE
authors: Melton
authors: John
authors: J
authors: Blangero
authors: Joanne E
authors: JE
authors: Curran
mesh_terms: D044466:Asian People / Q000235:genetics*; D001499:Bayes Theorem; D002489:Central America; D004272:DNA, Mitochondrial / Q000235:genetics*; D054629:Genome, Mitochondrial; D006239:Haplotypes; D006801:Humans; D007198:Indians, North American / Q000235:genetics*; D017058:Mexican Americans / Q000235:genetics*; D059645:Mutation Rate; D009656:North America; D010802:Phylogeny; D058974:Phylogeography; D012800:Siberia; D013020:South America
chemical_list: D004272:DNA, Mitochondrial