title: A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum.
abstract: C-type lectins (CTLs) are a group of proteins which bind to carbohydrate epitopes in the presence of Ca(2+), which have been described in a wide range of species. In this study, a cDNA sequence coding a putative CTL has been identified from the cDNA library constructed from the pig round worm Ascaris suum lung L3 (LL3) larvae, which was designated as A. suum C-type lectin-1 (As-CTL-1). The 510 nucleotide open reading frame of As-CTL-1 cDNA encoded the predicted 169 amino acid protein including a putative signal peptide of 23 residues and C-type lectin/C-type lectin-like domain (CLECT) at residue 26 to 167. As-CTL-1 was most similar to Toxocara canis C-type lectin-1 and 4 (Tc-CTL-1 and 4), and highly homologous to namatode CTLs and mammalian CTLs as well, such as human C-type lectin domain family 4 member G (CLECG4). In addition, As-CTL-1 was strongly expressed in tissue migrating LL3 and the L4 larvae, which were developmental larvae stages within the mammalian host. These results suggest that A. suum larvae might utilize As-CTL-1 to avoid pathogen recognition mechanisms in mammalian hosts due to it is similarity to host immune cell receptors.
authors: Department of Infectious Diseases, Division of Parasitology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. kukuri@med.miyazaki-u.ac.jp
authors: Ayako
authors: A
authors: Yoshida
authors: Eiji
authors: E
authors: Nagayasu
authors: Yoichiro
authors: Y
authors: Horii
authors: Haruhiko
authors: H
authors: Maruyama
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library; D007814:Larva / Q000378:metabolism*; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods; D017166:Toxocara canis / Q000737:chemistry
chemical_list: D037181:Lectins, C-Type; D012337:RNA, Ribosomal, 18S