title: An assay for detecting neutralization of rotavirus infection by quantitative determination of VP6 protein fluorescence intensity.
abstract: Previous rotavirus infection studies used the focus reduction assay extensively to evaluate cellular responses to viral infection, but this technique has a number of limitations. In this study, we developed a simplified, accurate rotavirus infection assay to evaluate the effects of inhibitory substances on rotavirus infection in vitro by measurement of the fluorescence intensities of stained cells.
authors: United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
authors: Xijier
authors: Mizuho
authors: M
authors: Inagaki
authors: Kengo
authors: K
authors: Kishita
authors: Tomio
authors: T
authors: Yabe
authors: Yoshihiro
authors: Y
authors: Kanamaru
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000378:metabolism; D009500:Neutralization Tests / Q000379:methods*; D012401:Rotavirus / Q000276:immunology*; D013050:Spectrometry, Fluorescence
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D007211:Indoles; C061332:VP6 protein, Rotavirus; C007293:DAPI; D016650:Fluorescein-5-isothiocyanate