title: Changes in activity of non-specific esterases in cadmium treated Lymantria dispar larvae.
abstract: Many biochemical, physiological and histological criteria have been used as indicators of exposures and effects of the contaminants. These changes can indicate the response of an organism to a specific environmental stressor. In the present paper, the effect of the acute and chronic exposure to cadmium as well as recovery from two cadmium concentrations (10 and 30 μgCd/g dry food) on gypsy moth (Lymantria dispar) midgut esterases was investigated. The influence of cadmium on trait plasticity was also examined. Esterases showed great sensitivity to low metal concentrations during acute and chronic treatments. Their activities during short-term exposure and after recovery significantly depended on cadmium concentrations. The esterases had greater index of plasticity during chronic treatments with 10 and 30 μgCd/dry food. Five esterase isoforms between 64 and 250 kDa were detected. Isoforms of esterases exposed to any of the two cadmium effects differed among several egg-masses. Isozymes were distinguished in one egg-mass during different cadmium treatments. We conclude that these enzymes could be considered potential and sensitive non-selective biomarkers for the presence of cadmium in food.
authors: Department of Insect Physiology and Biochemistry, University of Belgrade, Belgrade, Serbia. minavl@ibiss.bg.ac.rs
authors: Milena
authors: M
authors: Vlahović
authors: Vesna Perić
authors: VP
authors: Mataruga
authors: Larisa
authors: L
authors: Ilijin
authors: Marija
authors: M
authors: Mrdaković
authors: Dejan
authors: D
authors: Mirčić
authors: Dajana
authors: D
authors: Todorović
authors: Jelica
authors: J
authors: Lazarević
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000633:toxicity*; D043182:Carboxylesterase / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000201:enzymology; D007814:Larva / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009036:Moths / Q000502:physiology*; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
chemical_list: D019187:Cadmium Compounds; D004785:Environmental Pollutants; D043182:Carboxylesterase