title: Electrostatic interaction between inactivation ball and T1-S1 linker region of Kv1.4 channel.
abstract: Inactivation of potassium channels plays an important role in shaping the electrical signaling properties of nerve and muscle cells. The rapid inactivation of Kv1.4 has been assumed to be controlled by a "ball and chain" inactivation mechanism. Besides hydrophobic interaction between inactivation ball and the channel's inner pore, the electrostatic interaction has also been proved to participate in the "ball and chain" inactivation process of Kv1.4 channel. Based on the crystal structure of Kv1.2 channel, the acidic T1-S1 linker is indicated to be a candidate interacting with the positively charged hydrophilic region of the inactivation domain. In this study, through mutating the charged residues to amino acids of opposite polar, we identified the electrostatic interaction between the inactivation ball and the T1-S1 linker region of Kv1.4 channel. Inserting negatively charged peptide at the amino terminal of Kv1.4 channel further confirmed the electrostatic interaction between the two regions.
authors: Department of Physiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Zhuo
authors: Z
authors: Fan
authors: Xuying
authors: X
authors: Ji
authors: Mingyu
authors: M
authors: Fu
authors: Wanming
authors: W
authors: Zhang
authors: Du
authors: D
authors: Zhang
authors: Zhongju
authors: Z
authors: Xiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D057927:Hydrophobic and Hydrophilic Interactions; D015640:Ion Channel Gating; D051665:Kv1.4 Potassium Channel / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008322:Mammals; D008564:Membrane Potentials / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity
chemical_list: D051665:Kv1.4 Potassium Channel; D010455:Peptides; D011994:Recombinant Proteins