title: Interaction, uptake, and processing of LbL-coated microcarriers by PMNs.
abstract: Functionalized microcarriers or hollow capsules transporting active agents offer the opportunity for drug delivery inside cells. A promising application of these drug delivery systems is the direct transport as well as the release of immobilized antiinflammatory substances (AIS) into polymorphonuclear leukocytes (PMNs), which play a key role in the course of inflammatory processes. The intended delivery of AIS into the inflamed tissue could alleviate tissue destruction taking place during chronic inflammation, as well as facilitate the termination of these processes. In this study, the capability of functionalized CaCO(3) microcarriers as AIS transporter system targeted at PMNs is investigated. The time-dependent interaction of protamine sulfate and dextran sulfate multilayer-coated 5 μm ± 1 μm CaCO(3) carriers with PMNs, in comparison with the usage of SiO(2) carriers as monodisperse model system of defined sizes (1, 3, and 5 μm), reveals a sufficient carrier/cell interaction and uptake for coincubation periods between 2 and 24 h. Furthermore, the microcarriers are exposed to an environment simulating primary granule/phagosomal conditions after phagocytosis by means of PMN stimulation. The incubation of CaCO(3) microcarriers with cell supernatant demonstrates a partial multilayer decomposition (three to five layers) within 24 h, allowing the gradual release of AIS within the short PMN life span. This observation suggests a potential application for this drug delivery system inside immunologically active cells and may open the way to new alternatives in the treatment of chronic processes.
authors: Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Härtelstr. 16-18, 04107 Leipzig, Germany.
authors: Sophie
authors: S
authors: Rathmann
authors: Maria
authors: M
authors: Schönberg
authors: Jacqueline
authors: J
authors: Lessig
authors: Uta
authors: U
authors: Reibetanz
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism*; D002119:Calcium Carbonate / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000378:metabolism*; D016264:Dextran Sulfate / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008051:Lipid Bilayers; D018613:Microscopy, Confocal; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D011479:Protamines / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D008051:Lipid Bilayers; D011479:Protamines; D012822:Silicon Dioxide; D016264:Dextran Sulfate; D002119:Calcium Carbonate