title: [The proliferation of H1N1 subtype influenza viruses in A549 and BEAS-2B cells].
abstract: OBJECTIVE
  Analyze the proliferation of different host H1N1 subtype influenza viruses in A549 and BEAS-2B cells.
  
  
  METHODS
  Human, avain and swine three hosts of the H1N1 influenza viruses infected A549 and BEAS-2B cells and analyze the characteristics of different periods after inocubation. Determine the receptor binding specificity of influenza virus by hemagglutination (HA) test with RBCs with two types of receptor. And the receptors on surfaces of A549 and BEAS-2B cells were tested by flow cytometry.
  
  
  RESULTS
  The Cell Pathologic Effect (CPE) is obvious after 24 h inoculation in A549 cells by all the H1N1 influenza viruses, moreover, the peak hemagglutinin (HA) and 50% tissue culture cell infected dose (TCID50) titers was observed after 36 h of culturing in A549 cells. Otherwise, the CPE is not typical from 24 h-120 h inoculated by the same viruses and the HA, TCID50 titers were keep low all the periods in the BEAS-2B cell after inoculation. The receptor-binding preference of H1N1 viruses used in the study was screened by HA assay and some were found with 2-6-receptor binding affinity. Both SA a-2, 3Gal and SA a-2, 6Gal receptors were detected on A549 and BEAS-2B, furthermore, receptor density on A549 cells was significantly higher than that of BEAS-2B cells.
  
  
  CONCLUSIONS
  A549 cells were susceptible to human, avian and swine H1N1 influenza viruses infection and permissively for viral replication. However, BEASE-2B cells with similar receptor pattern and epithelium-derived propriety as A549 cells were unsusceptible to their infection and replication. Possible host factors involved in effective viral infection and replication were needed further study.
authors: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102202, China.
authors: Shu-Mei
authors: SM
authors: Zou
authors: Jian-Fang
authors: JF
authors: Zhou
authors: Zi
authors: Z
authors: Li
authors: Wen-Fei
authors: WF
authors: Zhu
authors: Ye
authors: Y
authors: Zhang
authors: Le-Ying
authors: LY
authors: Wen
authors: Yun
authors: Y
authors: Zhu
authors: Xiao-Dan
authors: XD
authors: Li
authors: Wei
authors: W
authors: Wang
authors: Yue-Long
authors: YL
authors: Shu
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002645:Chickens; D004285:Dogs; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*