title: Expression of vitellogenin in the testis and kidney of the spotted ray Torpedo marmorata exposed to 17β-estradiol.
abstract: In vertebrates, the liver was long thought to be the only site of vitellogenin (Vtg) production, but recent studies demonstrated that Vtg is also expressed in extrahepatic districts. The aim of this paper is to assess, by in situ hybridization and immunohistochemistry, the expression of Vtg in the testis and kidney of Torpedo marmorata exposed to 17β-estradiol (E(2)). In treated samples vtg mRNA and Vtg were detected contemporaneously only in the testis; differently the kidney cells were positive to Vtg antibody, but negative to vtg mRNA. This is the first study to assess that male germ cells, after an exposure to E(2), synthesize Vtg in a stage-dependent manner. The presence of Vtg and the modifications observed in the kidney after E(2) treatment are discussed.
authors: Department of Biological Sciences, Evolutionary and Comparative Biology Division, University of Naples Federico II, Naples, Italy.
authors: Giuseppina
authors: G
authors: Del Giudice
authors: Marina
authors: M
authors: Prisco
authors: Marisa
authors: M
authors: Agnese
authors: Mariailaria
authors: M
authors: Verderame
authors: Ermelinda
authors: E
authors: Limatola
authors: Piero
authors: P
authors: Andreuccetti
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014018:Tissue Distribution; D014101:Torpedo / Q000235:genetics* / Q000378:metabolism; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol