title: The involvement of down-regulation of Cdh1-APC in hippocampal neuronal apoptosis after global cerebral ischemia in rat.
abstract: Anaphase-promoting complex (APC) and its coactivator Cdh1 are required for maintaining cells in G1 phase of cell cycle in proliferating cells. Recent studies showed that Cdh1-APC was active in post-mitotic neurons, which regulates neuronal survival, differentiation, axonal growth and synaptic development. However, the possible function of Cdh1-APC in ischemic brain injury has not been determined. This study aimed to investigate changes in the activity of Cdh1-APC in hippocampus after global cerebral ischemia in rat. We found that, compared with sham group, the expression of Cdh1 in hippocampus was significantly decreased on 1 and 3 days of reperfusion in ischemia group (P<0.05), while neuronal apoptosis were found in hippocampal CA1 region and the two downstream substrates of Cdh1-APC (SnoN and Skp2) were significantly increased after global cerebral ischemia (P<0.05). This study demonstrates that the down-regulation of Cdh1-APC is associated with neuronal apoptosis in hippocampus following global cerebral ischemia. It brings a prospect to explore the further function of Cdh1-APC in the injured nervous system.
authors: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Yue
authors: Y
authors: Zhang
authors: Wenlong
authors: W
authors: Yao
authors: Jin
authors: J
authors: Qiu
authors: Wei
authors: W
authors: Qian
authors: Chang
authors: C
authors: Zhu
authors: Chuanhan
authors: C
authors: Zhang
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology*; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043743:Ubiquitin-Protein Ligase Complexes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000099274:Frizzled-Related Proteins
chemical_list: D064173:Anaphase-Promoting Complex-Cyclosome; D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D000099274:Frizzled-Related Proteins