title: Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue.
abstract: Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone, Oshsp26, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this study, we transferred this gene to tall fescue plants by an Agrobacterium-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern, and immunoblot analyzes. Compared to the control plants, the transgenic plants had significantly lower electrolyte leakage and accumulation of thiobarbituric acid-reactive substances when exposed to heat or methyl viologen. The photochemical efficiency of photosystem II (PSII) (Fv/Fm) in the transgenic tall fescue plants was higher than that in the control plants during heat stress (42°C). These results suggest that the OsHSP26 protein plays an important role in the protection of PSII during heat and oxidative stress in vivo.
authors: Division of Applied Life Sciences (BK21), IALS, PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea. coquf300@hotmail.com
authors: Kyung-Hee
authors: KH
authors: Kim
authors: Iftekhar
authors: I
authors: Alam
authors: Yong-Goo
authors: YG
authors: Kim
authors: Shamima Akhtar
authors: SA
authors: Sharmin
authors: Ki-Won
authors: KW
authors: Lee
authors: Sang-Hoon
authors: SH
authors: Lee
authors: Byung-Hyun
authors: BH
authors: Lee
mesh_terms: D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D031751:Festuca / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D015870:Gene Expression; D050888:Heat-Shock Proteins, Small / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D018384:Oxidative Stress; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D030821:Plants, Genetically Modified; D013312:Stress, Physiological
chemical_list: D050888:Heat-Shock Proteins, Small; D045332:Photosystem II Protein Complex