title: An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria.
abstract: Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.
authors: Department of Pharmacology, School of Medicine, University of California, Davis, CA 95616, USA. zamalik@ucdavis.edu
authors: Zulfiqar A
authors: ZA
authors: Malik
authors: Sumaira
authors: S
authors: Amir
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003889:Desert Climate; D006110:Grasshoppers / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D006458:Hemolymph / Q000737:chemistry / Q000201:enzymology; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight
chemical_list: D001053:Apolipoproteins; D019476:Insect Proteins; C042470:apolipophorin III