title: Characterization of the disposition of melamine in female Sprague-Dawley rats using ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: This study characterized the concentration-time profile of melamine in the heart, liver, spleen, lungs, kidneys, bladder, feces, urine, and plasma after melamine (MM) administration. Female Sprague-Dawley rats received a single oral dose of 1.0 g/kg body weight. Samples (n=4 per time point) were collected at 12, 24, 48, 72, 96, 120, 144, and 168 h. Based on calculations of the area under the concentration-time curves after dosing, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) was used to detect MM concentration in tissues. Peak concentrations of MM are reached in the livers and lungs at 12 h after dosing and in hearts, spleens, kidneys, bladders, feces, urine, and plasma at 24 h after dosing. More than 90% of the ingested MM is excreted in feces and urine within 24 h. These results provided initial understanding of the tissue disposition of MM. Moreover, this study demonstrates that UPLC-MS-MS can be used to detect MM in biological samples.
authors: Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Centre for Disease Control and Prevention, Shenzhen, 518060, China.
authors: Desheng
authors: D
authors: Wu
authors: Jianjun
authors: J
authors: Liu
authors: Qionghui
authors: Q
authors: Zhao
authors: Xinyun
authors: X
authors: Xu
authors: Linqing
authors: L
authors: Yang
authors: Haiyan
authors: H
authors: Huang
authors: Jianhui
authors: J
authors: Yuan
authors: Li
authors: L
authors: Zhou
authors: Zhixiong
authors: Z
authors: Zhuang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D005260:Female; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution; D014227:Triazines / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D001743:Urinary Bladder / Q000378:metabolism
chemical_list: D014227:Triazines; C011907:melamine