title: Role of damage-specific DNA polymerases in M13 phage mutagenesis induced by a major lipid peroxidation product trans-4-hydroxy-2-nonenal.
abstract: One of the major lipid peroxidation products trans-4-hydroxy-2-nonenal (HNE), forms cyclic propano- or ethenoadducts bearing six- or seven-carbon atom side chains to G>C≫A>T. To specify the role of SOS DNA polymerases in HNE-induced mutations, we tested survival and mutation spectra in the lacZα gene of M13mp18 phage, whose DNA was treated in vitro with HNE, and which was grown in uvrA(-)Escherichia coli strains, carrying one, two or all three SOS DNA polymerases. When Pol IV was the only DNA SOS polymerase in the bacterial host, survival of HNE-treated M13 DNA was similar to, but mutation frequency was lower than in the strain containing all SOS DNA polymerases. When only Pol II or Pol V were present in host bacteria, phage survival decreased dramatically. Simultaneously, mutation frequency was substantially increased, but exclusively in the strain carrying only Pol V, suggesting that induction of mutations by HNE is mainly dependent on Pol V. To determine the role of Pol II and Pol IV in HNE induced mutagenesis, Pol II or Pol IV were expressed together with Pol V. This resulted in decrease of mutation frequency, suggesting that both enzymes can compete with Pol V, and bypass HNE-DNA adducts in an error-free manner. However, HNE-DNA adducts were easily bypassed by Pol IV and only infrequently by Pol II. Mutation spectrum established for strains expressing only Pol V, showed that in uvrA(-) bacteria the frequency of base substitutions and recombination increased in relation to NER proficient strains, particularly mutations at adenine sites. Among base substitutions A:T→C:G, A:T→G:C, G:C→A:T and G:C→T:A prevailed. The results suggest that Pol V can infrequently bypass HNE-DNA adducts inducing mutations at G, C and A sites, while bypass by Pol IV and Pol II is error-free, but for Pol II infrequent.
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors: Beata
authors: B
authors: Janowska
authors: Dagmara
authors: D
authors: Kurpios-Piec
authors: Paulina
authors: P
authors: Prorok
authors: Grzegorz
authors: G
authors: Szparecki
authors: Marek
authors: M
authors: Komisarski
authors: Paweł
authors: P
authors: Kowalczyk
authors: Celina
authors: C
authors: Janion
authors: Barbara
authors: B
authors: Tudek
mesh_terms: D000447:Aldehydes / Q000633:toxicity*; D017104:Bacteriophage M13 / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018736:DNA Adducts / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D004257:DNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D019951:DNA Polymerase beta / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D015227:Lipid Peroxidation; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000187:drug effects; D059645:Mutation Rate; D017354:Point Mutation; D013014:SOS Response, Genetics
chemical_list: D000447:Aldehydes; D018736:DNA Adducts; D029968:Escherichia coli Proteins; C547259:4-hydroperoxy-2-nonenal; D004257:DNA Polymerase II; D019951:DNA Polymerase beta