title: Salivary tissue inhibitor of metalloproteinases-1 localization and glycosylation profile analysis.
abstract: Salivary tissue inhibitor of metalloproteinases-1 (TIMP-1) has been associated with pathological conditions in the oral cavity, but the origin of TIMP-1 in saliva remains unknown. Hence, we studied the localization of TIMP-1 in salivary gland tissue and also investigated if TIMP-1 found in blood and saliva is identical. Human salivary gland tissue samples (four parotid gland and four submandibular gland biopsies) were analysed for the presence of TIMP-1 mRNA and protein expression. To assess TIMP-1 glycosylation profiles in blood and saliva, the protein was isolated from plasma and unstimulated and stimulated whole saliva as well as stimulated parotid and submandibular saliva and analysed by MALDI-TOF mass spectrometry. TIMP-1 protein was demonstrated in mucous acinar cells of the submandibular gland and in ductal cells of both the parotid and submandibular gland. However, no TIMP-1 mRNA was detected in any of these cells. The glycosylation profiles of TIMP-1 isolated from whole saliva and saliva from the major glands were highly similar. In contrast, a significant difference was found between the glycoprofiles of salivary TIMP-1 and plasma TIMP-1. Although no clear evidence of TIMP-1 transcription in major salivary glands was demonstrated our results suggest that TIMP-1 in saliva does not originate from plasma.
authors: Department of Oral Medicine, Clinical Oral Physiology, Oral Pathology & Anatomy, Institute of Odontology, University of Copenhagen, Denmark. lhoa@life.ku.dk
authors: Lars
authors: L
authors: Holten-Andersen
authors: Morten
authors: M
authors: Thaysen-Andersen
authors: Siri Beier
authors: SB
authors: Jensen
authors: Christian
authors: C
authors: Buchwald
authors: Peter
authors: P
authors: Højrup
authors: Hanne
authors: H
authors: Offenberg
authors: Hans Jørgen
authors: HJ
authors: Nielsen
authors: Nils
authors: N
authors: Brünner
authors: Birgitte
authors: B
authors: Nauntofte
authors: Jesper
authors: J
authors: Reibel
mesh_terms: D000328:Adult; D005260:Female; D006031:Glycosylation; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D012463:Saliva / Q000737:chemistry*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1