title: Application of microextraction by packed sorbent to isolation of psychotropic drugs from human serum.
abstract: A method of microextraction by packed sorbent (MEPS) followed by liquid chromatography with diode array detection has been developed and optimized for the extraction of six tricyclic antidepressants (amitriptyline, nortriptyline, imipramine, desipramine, doxepin, nordoxepin) from human serum. The optimal parameters of MEPS extraction (type of sorbent, volume of sample, composition, and volume of washing and elution solutions) for these drugs in spiked samples were defined. The developed MEPS procedure was validated and then successfully applied to the analysis of serum reference material. The limit of detection (0.02-0.05 μg/mL), intraday (2.7-8.8%) and interday (4.4-11.6%) precision (RSD), and the accuracy of the assay (94.5-108.8%) at three concentration levels-0.2, 0.5, and 0.8 μg/mL-were estimated. The accuracy of the method was evaluated by the analysis of certified reference material. Moreover, the validated procedure was compared with the solid-phase extraction technique. Finally, microextraction by packed sorbent was assessed as a suitable tool in forensic and clinical methods for serum sample preparations.
authors: Department of Analytical Chemistry, Jagiellonian University, Kraków, Poland. wietecha@chemia.uj.edu.pl
authors: Renata
authors: R
authors: Wietecha-Posłuszny
authors: Aneta
authors: A
authors: Garbacik
authors: Michał
authors: M
authors: Woźniakiewicz
authors: Agnieszka
authors: A
authors: Moos
authors: Marcin
authors: M
authors: Wieczorek
authors: Paweł
authors: P
authors: Kościelniak
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D057230:Limit of Detection; D011619:Psychotropic Drugs / Q000097:blood* / Q000302:isolation & purification; D015203:Reproducibility of Results
chemical_list: D011619:Psychotropic Drugs