title: Catalytic solid substrate room temperature phosphorimetry for the determination of trace rhamnose based on its condensation reaction with calcein.
abstract: Calcein (R) could not only emit strong and stable room temperature phosphorescence (RTP) on filter paper using I(-) as perturber, but also could be oxidized by H(2)O(2) to form a non-phosphorescence compound (R'), resulting in the quenching of RTP signal of R. Moreover, the ortho-hydrogen of phenolic hydroxyl in R took condensation reaction with rhamnose (Rha) to produce non-phosphorescence compound (R-Rha) causing the RTP signal of R to further quench, and R-Rha was oxidized by H(2)O(2) to form R' and Rha, bringing about the sharp RTP signal quenching of R. Thus, a new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace Rha based on its strong catalytic effect on H(2)O(2) oxidizing R has been established, with the detection limit (LD) of 7.8zgspot(-1) (corresponding concentration: 2.0×10(-17) gm l(-1), sample volume: 0.40 μl spot(-1)). This method has been applied to determine trace Rha in cigarettes and jujubes, with the results coinciding well with those determined by a high performance liquid chromatography (HPLC). The component of R-Rha also was analyzed by means of HPLC, mass spectrometer and nuclear magnetic resonance (NMR) measurements. The mechanism of catalytic SSRTP for the determination of trace Rha was discussed.
authors: Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China. zzsyliujiaming@163.com
authors: Jia-Ming
authors: JM
authors: Liu
authors: Li-Ping
authors: LP
authors: Lin
authors: Hong-Xin
authors: HX
authors: Wang
authors: Shao-Qin
authors: SQ
authors: Lin
authors: Li-Hong
authors: LH
authors: Zhang
authors: Wen-Lian
authors: WL
authors: Cai
authors: Xuan
authors: X
authors: Lin
authors: You-Zhu
authors: YZ
authors: Pan
authors: Xin-Xing
authors: XX
authors: Wang
authors: Zhi-Ming
authors: ZM
authors: Li
authors: Li
authors: L
authors: Jiao
authors: Ma-Lin
authors: ML
authors: Cui
mesh_terms: D002384:Catalysis; D005452:Fluoresceins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D012210:Rhamnose / Q000032:analysis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D005452:Fluoresceins; D006861:Hydrogen Peroxide; D012210:Rhamnose; C007740:fluorexon