title: Selective biological response of human pulmonary microvascular endothelial cells and human pulmonary artery smooth muscle cells on cold-plasma-modified polyester vascular prostheses.
abstract: The aim of this work was to improve the hemocompatibility and the selectivity according to cells of non-woven poly(ethylene terephthalate) (PET) membranes. Non-woven PET membranes were modified by a combined plasma-chemical process. The surface of these materials was pre-activated by cold-plasma treatment and poly(acrylic acid) (PAA) was grafted by the in situ free radical polymerization of acrylic acid (AA). The extent of this reaction and the number of carboxylic groups incorporated were evaluated by colorimetric titration using toluidine blue O. All samples were characterized by SEM, AFM and thermogravimetric analysis, and the mechanical properties of the PAA grafted sample were determined. A selective cell response was observed when human pulmonary artery smooth muscle cells (HPASMC) or human pulmonary micro vascular endothelial cells (HPMEC) were seeded on the modified surfaces. HPASMC proliferation decreased about 60%, while HPMEC proliferation was just reduced about 10%. PAA grafted samples did not present hemolytic activity and the platelet adhesion decreased about 28% on PAA grafted surfaces.
authors: University Lille Nord de France, France. nicolas.blanchemain@univ-lille2.fr
authors: N
authors: N
authors: Blanchemain
authors: M R
authors: MR
authors: Aguilar
authors: F
authors: F
authors: Chai
authors: M
authors: M
authors: Jimenez
authors: E
authors: E
authors: Jean-Baptiste
authors: A
authors: A
authors: El-Achari
authors: B
authors: B
authors: Martel
authors: H F
authors: HF
authors: Hildebrand
authors: J San
authors: JS
authors: Roman
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003080:Cold Temperature; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D019544:Equipment Failure Analysis; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D055806:Microvessels / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D058626:Plasma Gases / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011093:Polyethylene Terephthalates; D011474:Prosthesis Design; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D058626:Plasma Gases; D011093:Polyethylene Terephthalates; D011092:Polyethylene Glycols