title: Marinobacter antarcticus sp. nov., a halotolerant bacterium isolated from Antarctic intertidal sandy sediment.
abstract: A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30(T), was isolated from Antarctic intertidal sandy sediment. The strain grew at 4-35 °C (optimum, 25 °C) and in 0-25% (w/v) NaCl (optimum, 3.0-4.0%). It could reduce nitrate to nitrite and hydrolyse Tween 80. The predominant cellular fatty acids of strain ZS2-30(T) were summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(16:0), C(18:1)ω9c, C(16:1)ω9c, C(12:0) 3-OH and C(12:0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. The genomic DNA G+C content of strain ZS2-30(T) was 55.8 mol%. Analyses of 16S rRNA gene sequences revealed that strain ZS2-30(T) was affiliated with the genus Marinobacter. It showed highest 16S rRNA gene sequence similarities to the type strains of three species of the genus Marinobacter, namely Marinobacter maritimus (98.3%), Marinobacter psychrophilus (98.1%) and Marinobacter goseongensis (97.1%), but the DNA-DNA relatedness values between strain ZS2-30(T) and the above three species were all lower than 45%. Moreover, strain ZS2-30(T) could be distinguished from closely related species of the genus Marinobacter by various phenotypic properties. Based on this taxonomic study using a polyphasic approach, strain ZS2-30(T) is considered to represent a novel species in the genus Marinobacter, for which the name Marinobacter antarcticus sp. nov. is proposed. The type strain of Marinobacter antarcticus is ZS2-30(T) ( = CGMCC 1.10835(T) = KCTC 23684(T)).
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Chang
authors: C
authors: Liu
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Chun-Xiao
authors: CX
authors: Chen
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Xi-Ying
authors: XY
authors: Zhang
authors: SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
authors: Yong
authors: Y
authors: Yu
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Ang
authors: A
authors: Liu
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Guo-Wei
authors: GW
authors: Li
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Xiu-Lan
authors: XL
authors: Chen
authors: SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
authors: Bo
authors: B
authors: Chen
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Bai-Cheng
authors: BC
authors: Zhou
authors: The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors: Yu-Zhong
authors: YZ
authors: Zhang
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S