title: The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase             arrest and apoptosis in YD-10B oral squamous carcinoma cells.
abstract: Histone acetylation is one of the key chromatin modifications that control             gene transcription during development and tumorigenesis. Recently, it was reported             that the histone deacetylase inhibitor, Trichostatin A (TSA), induces growth arrest             and apoptosis in tumors. However, the molecular mechanisms responsible for its             antitumor effects are not clear. The purpose of this study was to investigate             the effect of TSA on human oral squamous carcinoma cells and to determine the             mechanisms underlying the antitumor activity of TSA. MTT assays showed that TSA             inhibited cell proliferation in YD-10B cells. TSA also effectively arrested cell             cycle progression at the G2/M phase through the up-regulation of p21waf expression,             down-regulation of Cyclin B1 and reduction of the inhibitory phophorylation of             Cdc2. In addition, mitochondrial membrane destruction was induced by a 48 h TSA             treatment. TSA also induced cytochrome c release and proteolytic activation of             caspase 3 and caspase 7 in YD-10B cells. Taken together, these observations in             YD-10B oral cancer cells reveal the potential value of TSA in inhibiting oral             tumor growth.
authors: Department of Pathology, Chosun University College of Dentistry, Gwangju 501-759, Republic of Korea.
authors: Trinh Duc
authors: TD
authors: Anh
authors: Mee-Young
authors: MY
authors: Ahn
authors: Soo-A
authors: SA
authors: Kim
authors: Jung-Hoon
authors: JH
authors: Yoon
authors: Sang-Gun
authors: SG
authors: Ahn
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000201:enzymology*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A; D006655:Histone Deacetylases