title: Microbubble-mediated ultrasonic techniques for improved chemotherapeutic delivery in cancer.
abstract: BACKGROUND
  Ultrasound (US) exposed microbubble (MB) contrast agents have the capability to transiently enhance cell membrane permeability. Using this technique in cancer treatment to increase the efficiency of chemotherapy through passive, localized delivery has been an emerging area of research.
  
  
  OBJECTIVE
  Investigation of the influence of US parameters on MB-mediated drug delivery in cancer.
  
  
  METHODS
  The 2LMP breast cancer cells were used for in vitro experiments and 2LMP tumor-bearing mice were used during in vivo experiments. Changes in membrane permeability were investigated after the influence of MB-mediated US therapy parameters (i.e. frequency, mechanical index, pulse repetition period, US duration, and MB dosing and characteristics) on cancer cells. Calcein, a non-permeable fluorescent molecule, and Taxol, chemotherapeutic, were used to evaluate membrane permeability. Tumor response was also assessed histologically.
  
  
  RESULTS
  Combination chemotherapy and MB-mediated US therapy with optimized parameters increased cancer cell death by 50% over chemotherapy alone.
  
  
  CONCLUSIONS
  Increased cellular uptake of chemotherapeutic was dependent upon US system parameters.
  
  
  CONCLUSIONS
  Optimized MB-mediated US therapy has the potential to improve cancer patient response to therapy via increased localized drug uptake, which may lead to a lowering of chemotherapeutic drug dosages and systemic toxicity.
authors: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294-0019, USA.
authors: Anna G
authors: AG
authors: Sorace
authors: Jason M
authors: JM
authors: Warram
authors: Heidi
authors: H
authors: Umphrey
authors: Kenneth
authors: K
authors: Hoyt
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D045423:Microbubbles / Q000627:therapeutic use; D014407:Tumor Cells, Cultured; D014464:Ultrasonic Therapy / Q000379:methods*; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: D000970:Antineoplastic Agents