title: Anammox bacterial abundance, biodiversity and activity in a constructed wetland.
abstract: An integrated approach to document high anammox activity and biodiversity in a constructed wetland (CW) was performed and showed that substantial anammox activity could mitigate undesirable N(2)O emission. The enhanced anammox bacterial abundance, biodiversity and activity were achieved by supplementing activated sludge to the CW. Up to 3.38 × 10(7) gene copies g(-1) dry soil of anammox bacteria were enriched in the CW. The activity measured by isotope pairing technique increased from 1.6 nmol N g(-1) sludge h(-1) in the original activated sludge to 18 nmol N g(-1) soil h(-1) in the CW, with the specific cellular activity increased from 5.1 to 12.8 fmol cell(-1) d(-1). Up to 33% of produced N(2) could be attributed to anammox process in the CW, with the remainder being due to denitrification. Phylogenetic analysis of anammox bacterial 16S rRNA genes indicated a shift of community from single  Candidatus  "Brocadia fulgida" in sludge to multiple "Jettenia", "Brocadia", and "Anammoxoglobus" species in the CW. With static chambers and control experiments, the CW with supplemented sludge had a 30% reduced N(2)O emission flux compared with the tests without adding biomass during an 8 month testing period.
authors: State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China. gbzhu@rcees.ac.cn
authors: Guibing
authors: G
authors: Zhu
authors: Shanyun
authors: S
authors: Wang
authors: Xiaojuan
authors: X
authors: Feng
authors: Gaina
authors: G
authors: Fan
authors: Mike S M
authors: MS
authors: Jetten
authors: Chengqing
authors: C
authors: Yin
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D044822:Biodiversity; D018533:Biomass; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D053833:Wetlands
chemical_list: D000644:Quaternary Ammonium Compounds