title: Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage  defect management.
abstract: OBJECTIVE
  to validate isolation and cultivation methods of bone marrow mesenchymal stem cells (BM-MSCs) from iliac crest, and to compare biological characteristics of BM-MSCs from different age groups for preparation of autologous stem cell therapy in cartilage defect.
  
  
  METHODS
  patients undergoing spinal surgery were selected and grouped according to age. Iliac crest bone marrow from the patients was aspirated. BM-MSCs were isolated from the bone marrow and then cultivated. Their biological characteristics including morphological appearances and surface biomarkers were evaluated. Growth curves were observed. Sterility and Mycoplasma tests were also performed for quality assessment of BM-MSCs culture procedure.
  
  
  RESULTS
  in average, cultivated-BM-MSCs reached the number of 7.56-22.95 x 106 in 4-7 weeks period. BM-MSCs of all age groups showed the same quality of morphology, shape and surface biomarkers (CD105+, CD73+, CD34-, CD45-, CD14-, CD19-, HLA-DR-).
  
  
  CONCLUSIONS
  our procedures in isolating and cultivating of BM-MSCs have reached required amount for implantation into the cartilage lesion. In addition, the cultivated-BM-MSCs' biological characteristics were also in accordance with International Society of Cell Therapy (ISCT) MSCs criteria.
authors: Department of Surgery, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia. andri_lubis@yahoo.com
authors: Andri M T
authors: AM
authors: Lubis
authors: Lakshmi
authors: L
authors: Sandhow
authors: Vita K
authors: VK
authors: Lubis
authors: Alfiah
authors: A
authors: Noor
authors: Febrilian
authors: F
authors: Gumay
authors: Maurin
authors: M
authors: Merlina
authors: Wenny
authors: W
authors: Yang
authors: Yuyus
authors: Y
authors: Kusnadi
authors: Vinci
authors: V
authors: Lorensia
authors: Ferry
authors: F
authors: Sandra
authors: Nugroho H
authors: NH
authors: Susanto
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015703:Antigens, CD / Q000032:analysis*; D001854:Bone Marrow Cells; D002356:Cartilage / Q000293:injuries / Q000601:surgery; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003470:Culture Media; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007085:Ilium; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000382:microbiology; D008875:Middle Aged; D009174:Mycoplasma / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D003470:Culture Media; D006684:HLA-DR Antigens