title: Neuropeptide FF receptor modulates potassium currents in a dorsal root ganglion cell line.
abstract: This study investigated the presence of neuropeptide FF (NPFF) receptors on F-11 cells, a hybridoma derived from rat dorsal root ganglia (DRG) and mouse neuroblastoma. Binding experiments revealed a low density (4 fmol/mg) of high affinity (0.5 nM) [(3)H]-EYF binding sites in these cells. The whole-cell planar patch-clamp technique showed that dNPA, a selective NPFF(2) agonist, increased the voltage-dependent potassium outward currents (about 30 pA/pF) by 21%; this reversible effect on sustained delayed potassium currents is blocked by tetraethylammonium. The similar effects of NPFF and opioid agonists on K(+) currents in this cell line may explain their similar antinociceptive actions at the spinal level.
authors: Institut de Pharmacologie et de Biologie Structurale, CNRS/Université de Toulouse, UMR 5089, 205 route de Narbonne, 31077 Toulouse Cedex, France.
authors: Catherine
authors: C
authors: Mollereau
authors: Michel
authors: M
authors: Roumy
authors: Jean-Marie
authors: JM
authors: Zajac
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D006825:Hybridomas; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D018013:Receptors, Neuropeptide / Q000187:drug effects / Q000378:metabolism*; D019789:Tetraethylammonium / Q000494:pharmacology
chemical_list: D000701:Analgesics, Opioid; D009842:Oligopeptides; D015221:Potassium Channels; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor; D019789:Tetraethylammonium; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide