title: Halanaerobacter jeridensis sp. nov., isolated from a hypersaline lake.
abstract: An obligatory anaerobic, moderately halophilic bacterium, designated strain CEJFG43(T), was isolated from a sample of sediment collected below the salt crust on the hypersaline El Jerid lake, in southern Tunisia. The cells of this novel strain were Gram-staining-negative, non-sporulating, motile, short rods. They grew in media with 6-30% (w/v) NaCl (optimum 15%), at 20-60 °C (optimum 45 °C) and at pH 5.5-9.5 (optimum pH 8.3). The micro-organism fermented glucose, fructose, ribose, raffinose, galactose, mannose, sucrose, maltose, xylose, mannitol, pyruvate and glycerol. The products of glucose fermentation were lactate, ethanol, acetate, H(2) and CO(2). The genomic G+C DNA content of strain CEJFG43(T) was 33.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CEJFG43(T) belonged in the genus Halanaerobacter and was most closely related to Halanaerobacter lacunarum DSM 6640(T) (95.3% gene sequence similarity) and Halanaerobacter chitinivorans DSM 9569(T) (95.3%). The predominant cellular fatty acids were non-branched (C(16:0) and C(16:1)). Based on the phylogenetic and phenotypic evidence, strain CEJFG43(T) represents a novel species in the genus Halanaerobacter for which the name Halanaerobacter jeridensis sp. nov. is proposed. The type strain is CEJFG43(T) ( = DSM 23230(T) = JCM 16696(T)).
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.|Laboratoire des Bio-Procédés Environnementaux, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
authors: M
authors: M
authors: Mezghani
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.|Laboratoire des Bio-Procédés Environnementaux, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
authors: D
authors: D
authors: Alazard
authors: Laboratoire des Bio-Procédés Environnementaux, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
authors: F
authors: F
authors: Karray
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: J-L
authors: JL
authors: Cayol
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: M
authors: M
authors: Joseph
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: A
authors: A
authors: Postec
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: M-L
authors: ML
authors: Fardeau
authors: Aix-Marseille Univ., IRD, MIO UMR 235; CNRS/INSU, MIO UMR 7294, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors: J-L
authors: JL
authors: Tholozan
authors: Laboratoire des Bio-Procédés Environnementaux, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
authors: S
authors: S
authors: Sayadi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D014416:Tunisia; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride