title: Cryopreservation of boar semen by egg yolk-based extenders containing lactose or fructose is better than sorbitol.
abstract: The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen.
authors: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Panida
authors: P
authors: Chanapiwat
authors: Kampon
authors: K
authors: Kaeoket
authors: Padet
authors: P
authors: Tummaruk
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology; D004530:Egg Yolk / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry / Q000494:pharmacology*; D007785:Lactose / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013012:Sorbitol / Q000737:chemistry / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013552:Swine / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents; D005632:Fructose; D013012:Sorbitol; D007785:Lactose