title: Electrophoretic serum protein fraction profile during the different physiological phases in Comisana ewes.
abstract: The aim of this study was to evaluate the influence of different physiological phases on serum total proteins and their fractions of ten Comisana ewes housed in Mediterranean area. From each animal, blood samples were collected at different physiological phases: late pregnancy, post-partum, early, mid-, end lactation and dry period. On all samples serum total proteins were determined by the biuret method, and albumin, α-globulins, β(1) -globulins, β(2) -globulins and γ-globulins concentrations were assessed using an automated system. One-way repeated measures analysis of variance was applied to determine the significant effect of different physiological phases on the parameters studied. During the late pregnancy and post-partum, total proteins, β1- and β2-globulins and γ-globulins showed the highest values. Starting from post-partum, α-globulins increased to reach their peaks in mid-lactation. Early lactation was characterized by low γ-globulins values. The increase in serum albumin concentration and the drop in some globulin fractions determined the significant increase in albumin/globulin ratio. The obtained results contributed to improve the knowledge on electrophoretic profile during the different physiological phases in ewes, confirming that pregnancy and lactation periods affect the protein metabolism. Particularly, serum protein fractions pattern could give information about dehydration, plasma volume expansion and hepatic function, which occur during the different physiological phases. Dynamics of the protein profile - from pregnancy to dry period - which are provided by our results, could be considered as guidelines for the management strategies to guarantee the nutritional needs of these animals during the different physiological phases and to avoid a decline of productive performance and consequently an economic loss.
authors: Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy. giuseppe.piccione@unime.it
authors: G
authors: G
authors: Piccione
authors: D
authors: D
authors: Alberghina
authors: S
authors: S
authors: Marafioti
authors: C
authors: C
authors: Giannetto
authors: S
authors: S
authors: Casella
authors: A
authors: A
authors: Assenza
authors: F
authors: F
authors: Fazio
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis; D000818:Animals; D001609:Beta-Globulins / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods / Q000662:veterinary*; D005260:Female; D007774:Lactation / Q000097:blood; D049590:Postpartum Period / Q000097:blood; D011247:Pregnancy; D012709:Serum Albumin / Q000032:analysis; D012756:Sheep / Q000097:blood* / Q000502:physiology*; D005719:gamma-Globulins / Q000032:analysis
chemical_list: D000510:Alpha-Globulins; D001609:Beta-Globulins; D001798:Blood Proteins; D012709:Serum Albumin; D005719:gamma-Globulins