title: Polymorphisms  associated with egg number at 300 days of age in chickens.
abstract: We looked for variations that could be  associated with chicken egg number at 300 days of age (EN300) in seven genes of  the hypothalamic-pituitary-gonadal axis, including gonadotrophin-releasing  hormone-I (GnRH-I), GnRH receptor (GnRHR), neuropeptide Y (NPY),  dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP),  VIP receptor-1 (VIPR-1), prolactin (PRL), and the QTL region  between 87 and 105 cM of the Z chromosome. Ten mutations in the seven genes  were chosen to do marker-trait association analyses in a population comprising  1310 chickens, which were obtained from a company located in Guangdong Province  of China. The C1704887T of VIPR-1 was found to have a highly significant  association with EN300. The T5841629C of DRD2 and the C1715301T of VIPR-1 were significantly associated with EN300. A highly significant association  was also found between the C1704887T-C1715301T haplotypes of VIPR-1 and  EN300. H1H3 had the highest EN300. Four PCR-RFLP variations in the candidate  QTL region were selected to investigate their genetic effects on EN300. The  haplotypes of T32742468C-G32742603A in this region showed a highly significant  association with EN300. Bioinformatics analyses showed that both T32742468C  and G32742603A were located in intron 1 of the SH3-domain GRB2-like 2 (SH3GL2)  gene. We conclude that five SNPs, including C1704887T and C1715301T of VIPR-1,  T5841629C of DRD2, and T32742468C and G32742603A of SH3GL2, would  be useful as markers for breeding to increase chicken EN300.
authors: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors: H P
authors: HP
authors: Xu
authors: H
authors: H
authors: Zeng
authors: D X
authors: DX
authors: Zhang
authors: X L
authors: XL
authors: Jia
authors: C L
authors: CL
authors: Luo
authors: M X
authors: MX
authors: Fang
authors: Q H
authors: QH
authors: Nie
authors: X Q
authors: XQ
authors: Zhang
mesh_terms: D000375:Aging; D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007030:Hypothalamo-Hypophyseal System; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D010063:Ovum; D011110:Polymorphism, Genetic; D011388:Prolactin / Q000235:genetics / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000378:metabolism; D011966:Receptors, LHRH / Q000235:genetics / Q000378:metabolism; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000235:genetics / Q000378:metabolism; D012730:Sex Chromosomes / Q000235:genetics*; D014660:Vasoactive Intestinal Peptide / Q000235:genetics / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D017448:Receptors, Dopamine D2; D011966:Receptors, LHRH; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin