title: Use of multilocus variable-number tandem repeat analysis in molecular subtyping of Salmonella enterica serovar Typhi isolates.
abstract: We evaluated 11 variable number tandem repeat (VNTR) markers for the epidemiological investigation of Salmonella enterica serovar Typhi (S. Typhi) infection and compared the results to those obtained by PFGE. PFGE, using one or two restriction enzymes (XbaI and BlnI), was insufficient to differentiate between some isolates that were epidemiologically unlinked. Multilocus variable-number tandem repeat analysis (MLVA)-8, based on analysis of the eight most variable VNTRs, displayed a high level of discrimination when distinguishing between epidemiologically unlinked isolates that could not be discerned by PFGE with two enzymes. An MLVA-8 typing scheme could be implemented as a routine subtyping tool for the epidemiological investigation of S. Typhi infections. Because seven of the 11 VNTRs are highly variable, the VNTR markers may only be useful in determining genetic relationships among very closely related isolates in short-term epidemiological studies and not for discerning S. Typhi clones.
authors: Department of International Trade, Tamkang University, New Taipei, Taiwan, ROC.
authors: Yung-Yen
authors: YY
authors: Tien
authors: Aino Health Science Center, Aino University, Tokyo, Japan.
authors: Hiroshi
authors: H
authors: Ushijima
authors: Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors: Masashi
authors: M
authors: Mizuguchi
authors: The Central Region Laboratory, Center for Research and Diagnostics, Centers for Disease Control, Taichung, Taiwan, ROC.
authors: Shiu-Yun
authors: SY
authors: Liang
authors: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC.|The Central Region Laboratory, Center for Research and Diagnostics, Centers for Disease Control, Taichung, Taiwan, ROC.
authors: Chien-Shun
authors: CS
authors: Chiou
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D012485:Salmonella typhi / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial