title: SEM observation of wet biological specimens pretreated with room-temperature ionic liquid.
abstract: A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.
authors: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. ttsuda@chem.eng.osaka-u.ac.jp
authors: Tetsuya
authors: T
authors: Tsuda
authors: Noriko
authors: N
authors: Nemoto
authors: Koshi
authors: K
authors: Kawakami
authors: Eiko
authors: E
authors: Mochizuki
authors: Shoko
authors: S
authors: Kishida
authors: Takako
authors: T
authors: Tajiri
authors: Toshihiro
authors: T
authors: Kushibiki
authors: Susumu
authors: S
authors: Kuwabata
mesh_terms: D000818:Animals; D002460:Cell Line; D006046:Gold / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D013696:Temperature
chemical_list: D052578:Ionic Liquids; D006046:Gold