title: AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition.
abstract: The two structural domains of p529, a predicted AAA ATPase of Acidianus two-tailed virus (ATV), were expressed and purified. The N-terminal domain was demonstrated by loss-of-function mutations to carry ATPase activity with a temperature optimum of 60°C. This domain also showed DNA binding activity that was stronger for the whole protein and was weakened in the presence of ATP. The C-terminal domain exhibits Mg(2+)-dependent endonuclease activity that was eliminated by site-directed mutagenesis at a conserved catalytic PD…D/ExK motif. p529 pull-down experiments with cell extracts of Sulfolobus solfataricus demonstrated a specific interaction with Sso1273, corresponding to OppA(Ss), an N-linked glycoprotein that specifically binds oligopeptides. The sso1273 gene lies in an operon encoding an oligopeptide/dipeptide ABC transporter system. It is proposed that p529 is involved in ATV-host cell receptor recognition and possibly the endonuclease activity is required for cleavage of the circular viral DNA prior to cell entry.
authors: Archaea Centre, Department of Biology, University of Copenhagen, DK-2200, Copenhagen N, Denmark.
authors: Susanne
authors: S
authors: Erdmann
authors: Urte
authors: U
authors: Scheele
authors: Roger A
authors: RA
authors: Garrett
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D004267:DNA Viruses / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D011485:Protein Binding; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D016944:Sulfolobus / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D011991:Receptors, Virus; D014764:Viral Proteins; D000251:Adenosine Triphosphatases