title: Robust ordered mRNA differential display: an improved method for global gene expression profiling.
abstract: Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called "robust ordered mRNA differential display (RoDD)" by combining mRNA differential display (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers and a poly (dT:A) replacement technique. Redundancy was minimized by bead-based isolation and coverage was improved by using restriction enzymes that recognized 4-bp sites. This method offers the common virtues of gel-based methods along with the reliability of cDNA-AFLP. The most significant advantage of RoDD over current gel-based methods is greatly improved coverage and minimized redundancy.
authors: State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, China.
authors: Honggao
authors: H
authors: Liu
authors: Jihong
authors: J
authors: Hu
authors: Lei
authors: L
authors: Pan
authors: Shuzhen
authors: S
authors: Wang
authors: Ying
authors: Y
authors: He
authors: Yi
authors: Y
authors: Ding
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011061:Poly A / Q000235:genetics; D011071:Poly T / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011061:Poly A; D011071:Poly T; C008950:poly A-T; D004262:DNA Restriction Enzymes