title: Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.
abstract: We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.
authors: Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense C, Denmark. ujustesen@health.sdu.dk
authors: Ulrik Stenz
authors: US
authors: Justesen
authors: Anette
authors: A
authors: Holm
authors: Elisa
authors: E
authors: Knudsen
authors: Line Bisgaard
authors: LB
authors: Andersen
authors: Thøger Gorm
authors: TG
authors: Jensen
authors: Michael
authors: M
authors: Kemp
authors: Marianne Nielsine
authors: MN
authors: Skov
authors: Bente
authors: B
authors: Gahrn-Hansen
authors: Jens Kjølseth
authors: JK
authors: Møller
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*; D013997:Time Factors