title: Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes.
abstract: OBJECTIVE
  To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring.
  
  
  METHODS
  Randomized research experimental study.
  
  
  METHODS
  Academic basic research laboratory.
  
  
  METHODS
  Mice.
  
  
  METHODS
  Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring.
  
  
  METHODS
  Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19.
  
  
  RESULTS
  Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3.
  
  
  CONCLUSIONS
  Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring.
authors: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Xing-Wei
authors: XW
authors: Liang
authors: Zhao-Jia
authors: ZJ
authors: Ge
authors: Lei
authors: L
authors: Guo
authors: Shi-Ming
authors: SM
authors: Luo
authors: Zhi-Ming
authors: ZM
authors: Han
authors: Heide
authors: H
authors: Schatten
authors: Qing-Yuan
authors: QY
authors: Sun
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000235:genetics*; D019175:DNA Methylation / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D018392:Genomic Imprinting / Q000502:physiology*; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008098:Litter Size / Q000235:genetics / Q000502:physiology; D008183:Luteal Phase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D054315:Oocyte Retrieval; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA
chemical_list: D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse