title: The effect of sevoflurane on the expression of M1 acetylcholine receptor in the hippocampus and cognitive function of aged rats.
abstract: Our aim is to investigate the effect of 1.5 and 3.0% sevoflurane on the expression of M(1) acetylcholine receptor (mAChR M(1)) in the hippocampus and the cognitive function of aged rats. Forty Sprague-Dawley (SD) rats of 12-month old were randomly divided into five groups. All SD rats received 1.5 or 3.0% sevoflurane in a special glass anesthesia box for 2 h, respectively, except for the normal control group. Y-maze was used to test the ability of learning and memory after being received sevoflurane for 1 or 7 days at the same moment portion. The expression of mAChR M(1) in the hippocampus of rats was tested by RT-PCR. The results showed that 3% sevoflurane induced the decline of cognitive function and significantly decreased the mAChR M(1) expression at mRNA levels at 1 day in the 3.0% sevoflurane I group when compared with the normal control group. However, there was no significant difference among the other groups when compared with normal control group. Therefore, administration of sevoflurane might temporally affect the ability of cognitive function of rats through suppressing the mAChR M(1) expression at mRNA levels in hippocampus.
authors: Department of Anesthesiology, Affiliated No.4 Hospital of Soochow University, Wuxi, China.
authors: Sheng
authors: S
authors: Peng
authors: Yan
authors: Y
authors: Zhang
authors: Guo-Jun
authors: GJ
authors: Li
authors: Deng-Xin
authors: DX
authors: Zhang
authors: Da-Peng
authors: DP
authors: Sun
authors: Qiang
authors: Q
authors: Fang
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D003071:Cognition / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects; D008738:Methyl Ethers / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D043643:Receptor, Muscarinic M1 / Q000235:genetics / Q000378:metabolism*; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D012333:RNA, Messenger; D043643:Receptor, Muscarinic M1; D000077149:Sevoflurane