title: Silica-chitosan hybrid coating on Ti for controlled release of growth factors.
abstract: A hybrid material composed of a silica xerogel and chitosan was coated on Ti for the delivery of growth-factors. Fibroblast growth factor (FGF) and green fluorescence protein were incorporated into the coatings for hard tissue engineering. Silica was chosen as a coating material because of its high surface area as well as its good bioactivity. Chitosan provides mechanical stability and contributes to the control of the release rate of the growth factors. When the chitosan composition was 30% or more, the hybrid coating was stable physically and mechanically. The release of the growth-factors, observed in phosphate buffer solution at 37°C, was strongly dependent on the coating material. The hybrid coating containing FGF showed significantly improved osteoblast cell responses compared to the pure xerogel coating with FGF or the hybrid coating without FGF. These results indicate that the hybrid coating is potentially very useful in enhancing the bioactivity of metallic implants by delivering growth-factors in a controlled manner.
authors: Department of Materials Science and Engineering, Seoul National University, Seoul, Korea.
authors: Shin-Hee
authors: SH
authors: Jun
authors: Eun-Jung
authors: EJ
authors: Lee
authors: Hyoun-Ee
authors: HE
authors: Kim
authors: Jun-Hyeog
authors: JH
authors: Jang
authors: Young-Hag
authors: YH
authors: Koh
mesh_terms: D016475:3T3 Cells; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005346:Fibroblast Growth Factors / Q000008:administration & dosage; D005782:Gels; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors; D012822:Silicon Dioxide; D048271:Chitosan; D014025:Titanium