title: Rat lymphocytes express NMDA receptors that take part in regulation of cytokine production.
abstract: Incubation of rat lymphocytes with homocysteine (HC) or homocysteic acid (HCA) was found to increase the stationary levels of free radicals in lymphocytes, the effect of both ligands being mediated by ionotropic receptors activated by N-methyl-D-aspactic acid (NMDA), the expression of which on rat lymphocyte membranes was earlier demonstrated. In agreement with these data, increase of free radicals in the lymphocyte cytoplasm is preceded by an increase in the intracellular calcium levels, activation of protein kinase C, nicotinamide adenine dinucleotide phosphate oxidase and/or nitric oxide synthase. Both HC and HCA increase the production of IFN-γ and TNF-α by lymphocytes and antagonist of NMDA receptors; MK-801 prevents this effect. The data presented show that rat lymphocyte membrane contains functionally active NMDA receptors, which regulate cytokine accumulation.
authors: Department of Biochemistry, MV Lomonosov Moscow state University, Moscow, Russia. mather03@gmail.com
authors: Elizaveta
authors: E
authors: Vladychenskaya
authors: Olga
authors: O
authors: Tyulina
authors: Shiro
authors: S
authors: Urano
authors: Alexander
authors: A
authors: Boldyrev
mesh_terms: D000818:Animals; D053119:Benzophenanthridines / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology; D005434:Flow Cytometry; D005453:Fluorescence; D005609:Free Radicals / Q000378:metabolism*; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D019255:NADPH Oxidases / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053119:Benzophenanthridines; D016207:Cytokines; D005609:Free Radicals; D016194:Receptors, N-Methyl-D-Aspartate; D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine; C007956:homocysteic acid; D009569:Nitric Oxide; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D007371:Interferon-gamma; C016299:chelerythrine; D019255:NADPH Oxidases; D011493:Protein Kinase C; D002118:Calcium