title: Synthesis of silver nanoparticle-hollow titanium phosphate sphere hybrid as a label for ultrasensitive electrochemical detection of human interleukin-6.
abstract: A silver nanoparticle-hollow titanium phosphate sphere (AgNP-TiP) hybrid is successfully synthesized and used as a label for electrochemical detection of human interleukin-6 (IL-6). Hollow TiP spheres with a diameter of 430 nm and an average thickness of 40 nm are synthesized by a template approach. The AgNPs are incorporated in situ into the TiP shell via an exchange process. The as-prepared AgNP-TiP hybrid shows outstanding biocompatibility, good dispersity and solubility in water, and high silver loading properties (289.2 mg of silver in 1.0 g of TiP). These advantages make the AgNP-TiP hybrid an effective candidate as an amplification label in immunoassay systems. Herein, the as-prepared AgNP-TiP hybrid is attached to a signal antibody (Ab(2) ) to produce Ab(2) -AgNP-TiP labels in the fabrication of an electrochemical immunosensor. The nanoparticle-based amplification labels, upon coupling with a magnetic sensing array, give rise to an extremely sensitive response to IL-6 in a linear range of 0.0005-10 ng mL(-1)  with a detection limit of 0.1 pg mL(-1) . The proposed sensor exhibits high specificity, good reproducibility, and long-term stability, and may be a promising technique for protein and DNA detection.
authors: School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
authors: Juan
authors: J
authors: Peng
authors: Li-Na
authors: LN
authors: Feng
authors: Zhong-Jie
authors: ZJ
authors: Ren
authors: Li-Ping
authors: LP
authors: Jiang
authors: Jun-Jie
authors: JJ
authors: Zhu
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D015850:Interleukin-6 / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012834:Silver / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry
chemical_list: D015850:Interleukin-6; C036532:titanium phosphate; D012834:Silver; D014025:Titanium