title: Two non-vesicular ATP release pathways in the mouse erythrocyte membrane.
abstract: Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1(-/-) mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1(-/-) mouse erythrocytes. In contrast to Panx1(+/+) cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.
authors: Department of Physiology and Biophysics, University of Miami, School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA.
authors: Feng
authors: F
authors: Qiu
authors: Junjie
authors: J
authors: Wang
authors: David C
authors: DC
authors: Spray
authors: Eliana
authors: E
authors: Scemes
authors: Gerhard
authors: G
authors: Dahl
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004396:Coloring Agents / Q000378:metabolism; D017630:Connexins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D004176:Dipyridamole / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D055786:Gene Knockout Techniques; D005905:Glyburide / Q000494:pharmacology; D006801:Humans; D016285:Iloprost / Q000494:pharmacology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D017630:Connexins; D009419:Nerve Tissue Proteins; C503631:Panx1 protein, mouse; D004176:Dipyridamole; D000255:Adenosine Triphosphate; D016285:Iloprost; D005905:Glyburide