title: Acacetin induces apoptosis in human T cell leukemia Jurkat cells via             activation of a caspase cascade.
abstract: Flavonoids are naturally occurring antioxidants, with several flavonoids             shown to have chemopreventive effects on cancer. We investigated the effects of             the flavonoid acacetin on human T cell leukemia Jurkat cells. Acacetin inhibited             the proliferation of Jurkat cells by inducing apoptosis in a concentration- and             time-dependent manner. Acacetin-induced cell death was characterized by changes             in nuclear and cell morphology. Treatment of Jurkat cells with acacetin also induced             caspase-3, -8 and -9 activities in a time-dependent manner. Acacetin-induced apoptosis             was blocked by a broad-spectrum caspase inhibitor, a caspase-3 inhibitor and a             caspase-8 inhibitor, but not by a caspase-9 inhibitor. In addition, acacetin promoted             the expression of FAF1, phosphor-FADD, Apaf-1 and cytochrome c. Acacetin-induced             apoptosis was also accompanied by upregulation of Bax, and downregulation of Bcl-2.             Taken together, these results suggest that acacetin may induce apoptosis in T             cell leukemia cells, possibly by activating the Fas-mediated pathway. These findings             may help in designing cancer therapeutic and chemopreventive agents.
authors: Department of Clinical Pharmacotherapeutics, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai 981-8553, Japan.
authors: Kiyotada
authors: K
authors: Watanabe
authors: Syu-Ichi
authors: S
authors: Kanno
authors: Ayako
authors: A
authors: Tomizawa
authors: Shin
authors: S
authors: Yomogida
authors: Masaaki
authors: M
authors: Ishikawa
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D020169:Caspases / Q000187:drug effects / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D053200:Fas-Associated Death Domain Protein / Q000378:metabolism; D047309:Flavones / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D053200:Fas-Associated Death Domain Protein; D047309:Flavones; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020169:Caspases; C023717:acacetin