title: Live-cell 3D super-resolution imaging in thick biological samples.
abstract: We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.
authors: Istituto Italiano di Tecnologia, Genova, Italia.
authors: Francesca
authors: F
authors: Cella Zanacchi
authors: Zeno
authors: Z
authors: Lavagnino
authors: Michela
authors: M
authors: Perrone Donnorso
authors: Alessio
authors: A
authors: Del Bue
authors: Laura
authors: L
authors: Furia
authors: Mario
authors: M
authors: Faretta
authors: Alberto
authors: A
authors: Diaspro
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D053769:Nanocapsules; D019047:Phantoms, Imaging; D018874:Spheroids, Cellular / Q000166:cytology*
chemical_list: D053769:Nanocapsules