title: Control of charcoal rot fungus Macrophomina phaseolina by extracts of Datura metel.
abstract: Methanolic leaf and fruit extracts of Datura metel were found highly effective in suppressing against Macrophomina phaseolina, the cause of charcoal rot disease. These extracts were further subjected to successive fractionation with n-hexane, chloroform, ethyl acetate and n-butanol. All the concentrations (3.125-200 mg mL⁻¹) of chloroform, ethyl acetate and n-butanol fractions of leaf extract, and n-hexane fraction of fruit extract completely inhibited the target fungal growth. Two compounds A and B from the n-hexane fraction of fruit extract and compound C from n-butanol fraction of leaf extract were obtained by TLC. Compound B exhibited the best antifungal activity with an MIC value of 7.81 µg mL⁻¹ that was at par with that of commercial fungicide mancozeb (80% w/w). This study concludes that M. phaseolina can be effectively controlled by natural antifungal compounds in n-hexane fraction of methanolic fruit extract of D. metel.
authors: Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan. arshadjpk@yahoo.com
authors: Arshad
authors: A
authors: Javaid
authors: Amna
authors: A
authors: Saddique
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D058530:Datura metel / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D006586:Hexanes; D010936:Plant Extracts; C026385:n-hexane; D020001:1-Butanol