title: [The serological and genetic identification of a CisAB blood sample].
abstract: OBJECTIVE
  To verify the DNA sequence of a sample serologically identified as CisAB.
  
  
  METHODS
  Forward and reverse group methods were used to determine the blood serological type of that the sample, and PCR-sequence specific primer (PCR-SSP) method was used for genotyping the sample.
  
  
  RESULTS
  Serologically, the forward group test showed that the sample was AB, while the reverse group test showed that the sample had the anti-B and anti-H  + + +. The auto antibodies were negative. PCR-SSP assay showed the sample was CisAB01. ABO genetic locus sequencing showed c.261delG in exon 6, c.297 was homozygous AA. Mutations c.467C to T and c.803G to C were found in exon 7. A novel heterozygous mutation, c.724G to T, was detected.
  
  
  CONCLUSIONS
  The serological phenotype of the specimen was CisAB. The genotype was ABO *CisAB01 and ABO *O01. A novel mutation c.724G to T in exon 7 was identified (GenBank accession no. JF304777).
authors: Jiangsu Province Blood Center, Nanjing, Jiangsu, People's Republic of China. lyc60@126.com
authors: Yan-chun
authors: YC
authors: Liu
authors: Ling
authors: L
authors: Ma
authors: Dong-min
authors: DM
authors: Zhang
authors: Ling
authors: L
authors: Zheng
authors: Yi
authors: Y
authors: Liu
authors: Min-hui
authors: MH
authors: Wu
authors: Min
authors: M
authors: Xue
authors: Wen-biao
authors: WB
authors: Liang
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics* / Q000378:metabolism*; D000483:Alleles; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D005091:Exons; D005838:Genotype; D006801:Humans; D010375:Pedigree
chemical_list: D000017:ABO Blood-Group System