title: Chemoselective and enantioselective analysis of proteinogenic amino acids utilizing N-derivatization and 1-D enantioselective anion-exchange chromatography in combination with tandem mass spectrometric detection.
abstract: D-Amino acid analysis in biological samples still poses a challenge to analytical chemists. In higher developed species trace amounts of d-amino acids have to be detected in vast excesses of the corresponding L-enantiomers. This method utilizes an easy-to-carry-out derivatization step on the amino group with an iron ferrocenyl propionate hydroxy succinimide ester followed by one-dimensional enantioselective anion exchange chromatography with cinchona alkaloid based chiral stationary phases (CSPs). MS detection is carried out in the highly sensitive SRM (selected reaction monitoring) mode, which allows a chemoselective differentiation of amino acid derivatives as well as their enantioselective separation in one step. Application of this method allows LOD (limits of detection) in the low μmol L(-1) range and baseline enantioseparation for all proteinogenic amino acids except for Pro, Arg and His. The D-enantiomers of isomeric Leu and Ile were separated chromatographically and pose an example for the complementary selectivities of LC and MS. A successful application of this procedure to unprocessed human urine indicated the eligibility to analyse biological samples.
authors: Department of Analytical Chemistry, University of Vienna, Vienna, Austria.
authors: Roland J
authors: RJ
authors: Reischl
authors: Lucie
authors: L
authors: Hartmanova
authors: Marina
authors: M
authors: Carrozzo
authors: Monika
authors: M
authors: Huszar
authors: Peter
authors: P
authors: Frühauf
authors: Wolfgang
authors: W
authors: Lindner
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000652:urine; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000596:Amino Acids