title: Metabolic modulation of cytokine-induced brain endothelial adhesion molecule expression.
abstract: OBJECTIVE
  Cytokines contribute to cerebro-vascular inflammatory and immune responses by inducing ECAMs' expression. Ischemic insults can be separated into aglycemic and hypoxic components. However, whether aglycemia, hypoxia or OGD plays a major role in dysregulating BBB or promotes immune cell infiltration via ECAMs' expression is not clear. We investigated how expression of ICAM-1, VCAM-1, MAdCAM-1, PECAM-1, E- and P-selectin in response to TNF-α, IL-1β and IFN-γ was altered by aglycemia (A), hypoxia (H) or combined oxygen glucose deprivation (OGD).
  
  
  METHODS
  A cell surface enzyme linked immunoabsorbent assay (cell surface ELISA) was used to analyze ECAM expression.
  
  
  RESULTS
  We observed that ICAM-1 and PECAM-1 expressions were insensitive to hypoxia, aglycemia or OGD. Conversely, VCAM-1 and E-selectin were increased by hypoxia, but not by aglycemia. MAdCAM-1 and P-selectin were induced by hypoxia, and decreased by aglycemia. Patterns of cytokine-regulated ECAMs' expression were also modified by metabolic conditions.
  
  
  CONCLUSIONS
  Our results indicate that patterns of inflammation-associated ECAMs represent cumulative influences from metabolic stressors, as well as cytokine activation. The expression of ECAMs following tissue injury reflects mechanistic interactions between metabolic disturbances, and alterations in tissue cytokines. Normalization of tissue metabolism, as well as cytokine profiles, may provide important targets for therapeutic treatment of inflammation.
authors: Departments of Molecular and Cellular Physiology Cell Biology and Anatomy Neurology, LSU Health Sciences Center, Shreveport, LA 71130-3932, USA.
authors: Ganta Vijay
authors: GV
authors: Chaitanya
authors: Walter
authors: W
authors: Cromer
authors: Shannon
authors: S
authors: Wells
authors: Merilyn
authors: M
authors: Jennings
authors: James M
authors: JM
authors: Mathis
authors: Alireza
authors: A
authors: Minagar
authors: Jonathan Steven
authors: JS
authors: Alexander
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D013549:Sweetening Agents / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D013549:Sweetening Agents; D005947:Glucose