title: In vitro maturation: a five-year experience.
abstract: OBJECTIVE
  Although some patients may benefit from reduced follicle-stimulating hormone (FSH) application, in-vitro maturation (IVM) belongs to the rare treatment options in assisted reproduction. We summarize our five-year IVM experience.
  
  
  METHODS
  Retrospective, observational study.
  
  
  METHODS
  Reproductive Medicine, University Hospital.
  
  
  METHODS
  115 patients with polycystic ovary syndrome (PCOS) as well as patients after ovarian hyperstimulation syndrome (OHSS) from February 2005 to December 2009.
  
  
  METHODS
  Stimulation started between day 3-10 of the menstrual cycle and FSH dosage was 125IU/day over three days. Ovulation was induced on the third day of FSH injection or one day afterwards and oocyte retrieval was performed 33-38 hours later. Oocytes were cultivated for 24 hours in IVM medium. Fertilization was carried out one day after oocyte retrieval and embryo transfer two days afterwards.
  
  
  METHODS
  Pregnancy rates.
  
  
  RESULTS
  115 patients were included and 215 oocyte retrievals (intracytoplasmic sperm injection: n=125, 59%; in vitro fertilization: n=73, 34.4%) with 177 embryo transfers performed. The main reasons for IVM were: PCOS (71.7%) and OHSS (15.0%). Mean number of oocytes was 8.9/oocyte retrieval with 5.9 (64%) becoming mature, 2.8 (45.1%) being fertilized and 2.1 transferred. Pregnancy rate per transfer was 15.3% (n=27) with 13 live births (7.3%), one intrauterine death (0.6%), four miscarriages (2.3%) and nine biochemical pregnancies (5.1%). In 61 cases, fertilized oocytes were frozen and 32 cryotransfers were performed, resulting in three pregnancies.
  
  
  CONCLUSIONS
  Although the pregnancy rate was low, IVM is very convenient for patients due to low FSH dosages and few appointments at low cost.
authors: Department of Gynecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
authors: Sabine
authors: S
authors: Roesner
authors: Department of Gynecological Endocrinology and Fertility Disorders, Inselspital Bern, Bern, Switzerland.
authors: Michael
authors: M
authors: VON Wolff
authors: Department of Gynecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
authors: Ingeborg
authors: I
authors: Eberhardt
authors: Department of Gynecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
authors: Petra
authors: P
authors: Beuter-Winkler
authors: Department of Gynecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
authors: Bettina
authors: B
authors: Toth
authors: Department of Gynecological Endocrinology and Fertility Disorders, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
authors: Thomas
authors: T
authors: Strowitzki
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy*; D016471:Ovarian Hyperstimulation Syndrome / Q000150:complications; D011085:Polycystic Ovary Syndrome / Q000150:complications; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies; D016896:Treatment Outcome