title: Chemoselective fabrication of high density peptide microarray by hetero-bifunctional tetra(ethylene glycol) linker for click chemistry conjugation.
abstract: A hetero-bifunctional tetra(ethylene glycol) molecule with silane and azide termini was synthesized, and this molecule was used to prepare azide-derivatized glass surface in one step. The resulting glass surface was available for fabricating peptide microarray by the conjugation with alkyne-containing peptide using click chemistry, which proceeded to the completion at low temperature and in aqueous solution. A high density of peptide on the surface was achieved due to concise overall procedure and highly efficient conjugation reaction. Immobilized peptides were highly bio-functional on the surface, as demonstrated by the ability to detect protease activity. Due to the biologically orthogonal manner of conjugation, peptide conjugated by site-specific immobilization was more accessible by protease than that conjugated by random amide conjugation. This site-specific and high efficient immobilization technique could be expanded to large scale development of biocompatible peptide and protein arrays for use in various applications.
authors: Department of Bioengineering, Bourns College of Engineering, University of California, Riverside, California 92521, USA.
authors: Yongfeng
authors: Y
authors: Zhao
authors: Yan
authors: Y
authors: Liu
authors: Ilkeun
authors: I
authors: Lee
authors: Yang
authors: Y
authors: Song
authors: Xiangdong
authors: X
authors: Qin
authors: Francisco
authors: F
authors: Zaera
authors: Jiayu
authors: J
authors: Liao
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000494:pharmacology; D057930:Click Chemistry / Q000379:methods*; D019855:Ethylene Glycol / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry; D013499:Surface Properties / Q000187:drug effects; D014357:Trypsin / Q000378:metabolism
chemical_list: D000480:Alkynes; D001672:Biocompatible Materials; D010455:Peptides; D012821:Silanes; D011092:Polyethylene Glycols; D014357:Trypsin; D019855:Ethylene Glycol