title: Genotyping and antifungal drug susceptibility of Trichosporon asahii isolated from Chinese patients.
abstract: As a major pathogenic agent of trichosporonosis, Trichosporon                         asahii can cause life-threatening infection in immunocompromised patients. In this study, we analyzed the genotypes of the intergenic spacer (IGS) 1 region of the rRNA gene and the antifungal drug susceptibility of eight T. asahii isolates obtained from Chinese patients. Five genotypes were identified from the eight isolates, including three novel genotypes, three genotype 1, and two genotype 4. The eight T. asahii isolates were resistant to amphotericin B, 5-flucytosine, and terbinafine, but were highly sensitive to fluconazole (FLC), itraconazole (ITC), and voriconazole (VRC). The mean minimum inhibitory concentrations (MICs) of FLC and VRC were significantly lower than those reported in most other countries, while that of ITC was slightly higher. Our results suggest that genotypes of the T. asahii isolated from China are different from those of other countries, and azole drugs appeared to be more effective on the Chinese isolates. These results provide new insights into the epidemiology and antifungal treatment for T. asahii.
authors: Department of Dermatology, The Military General Hospital of Beijing PLA, Dongcheng District, Beijing, People's Republic of China.
authors: Zhikuan
authors: Z
authors: Xia
authors: Rongya
authors: R
authors: Yang
authors: Wenling
authors: W
authors: Wang
authors: Lin
authors: L
authors: Cong
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002681:China; D005656:Fungal Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D014250:Trichosporon / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D060586:Trichosporonosis / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins