title: Comparability of two different polyacrylamide gel electrophoresis methods for the classification of LDL pattern type.
abstract: BACKGROUND
  The measurement of small dense low-density lipoprotein (sdLDL) particles is relevant when assessing cardiovascular risk. However, there is as yet no referenced method for the determination of LDL subfractions or a standardized comparison of the methods currently available. Therefore, the aim of this study was to compare the pattern of LDL particles measured by polyacrylamide tube gel electrophoresis (PTGE) and polyacrylamide gradient gel electrophoresis (PGGE) and to correlate the results with triglyceride concentration.
  
  
  METHODS
  Serum samples were collected from 177 patients. Lipid profile and LDL particle size were assessed using PTGE and PGGE.
  
  
  RESULTS
  Pearson correlation and kappa index revealed a very good agreement between the methods. There was 81.3% concordance for classification of sdLDL particles and 97.2% concordance for classification of large LDL when PTGE and PGGE were compared. LDL size correlated with triglyceride in subjects with triglyceride levels >116 mg/dl, pointing to a high CAD risk, as reflected by their higher prevalence of pattern B.
  
  
  CONCLUSIONS
  PTGE correlates favourably and is in very good agreement with PGGE. The determination of LDL particle size may be an appropriate analytical procedure to estimate CAD risk in patients with high triglyceride levels.
authors: Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain.
authors: Celia
authors: C
authors: Bañuls
authors: Lorena
authors: L
authors: Bellod
authors: Ana
authors: A
authors: Jover
authors: Maria Luisa
authors: ML
authors: Martínez-Triguero
authors: Víctor Manuel
authors: VM
authors: Víctor
authors: Milagros
authors: M
authors: Rocha
authors: Antonio
authors: A
authors: Hernández-Mijares
mesh_terms: D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000145:classification*; D008297:Male; D008875:Middle Aged; D010316:Particle Size
chemical_list: D008077:Lipoproteins, LDL