title: [The effect of RhoE on CD44 promoter and the malignant behaviors of colorectal cancer cell].
abstract: OBJECTIVE
  To study the effect of RhoE on the transcriptional regulation of cd44 and in vivo tumorigenicity of nude mice.
  
  
  METHODS
  cd44 promoter was amplified from human embryonic kidney HEK293 cells with PCR and insert into Dual-Luciferase Reporter plasmid pGL3-Basic. After confirmed with sequence analysis, the generated recombinant was transfected into SW480 and LoVo cells to monitor their activity. Colon cancer SW480 and LoVo cells were cotransfected with pGL3-CD44 promoter along with pcDNA3. 1-RhoE and pcDNA3. 1 respectively. SW480 and LoVo cells were stably transfected with pcDNA3. 1-RhoE and the control group and were inoculated into nude mice to observe tumor growth. Immunohistochemistry assay was applied to observe the morphology of tumor cells and the expression of CD44 molecules.
  
  
  RESULTS
  The cd44 promoter sequence was amplified correctly, Dual-Luciferase Reporter Assay showed that the constructed reporter gene has promoter activity. The expression of cd44 promoter sequence containing reporter gene in pcDNA3. 1-RhoE expression positive LoVo cells was inhibited; HE staining demonstrated that the pcDNA3. 1-RhoE transfected tumor cells was significantly smaller than that in the control group, and consistent size and shape tumor cells were observed but no tumor giant cells, the corresponding volume of the tumor nuclei were also small.
  
  
  CONCLUSIONS
  RhoE could partially reverse the malignant biological behavior of tumors by inhibiting the transcriptional regulation of cd44 promoter.
authors: The Laboratory of Signal Transduction & Molecular Targeting Therapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hai-Jun
authors: HJ
authors: Zhou
authors: Li-Lan
authors: LL
authors: Li
authors: Cai-Xia
authors: CX
authors: Yue
authors: Wen
authors: W
authors: Wei
authors: Nan-Jing
authors: NJ
authors: Li
authors: Qiu-Lin
authors: QL
authors: Tang
authors: Feng
authors: F
authors: Bi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014162:Transfection; D020741:rho GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology
chemical_list: D018960:Hyaluronan Receptors; C508705:RND3 protein, human; D020741:rho GTP-Binding Proteins