title: Barrier protective activities of phloroglucinol on lipopolysaccharide (LPS)-induced barrier disruption in human endothelial cells.
abstract: Phloroglucinol plays an important role in oxidative stress, inflammatory responses, and matrix metalloproteinase (MMP) regulation. However, the barrier protective functions of phloroglucinol are not well studied. The objective of this study was to fill this gap. We did this by investigating the barrier protective activities of phloroglucinol on lipopolysaccharide (LPS)-induced barrier disruption in human endothelial cells measured by cellular permeability, monocytes adhesion, and migration toward human endothelial cells. The results showed that phloroglucinol inhibited LPS-induced barrier hyperpermeability, monocyte adhesion, and migration. These inhibitory effects were significantly correlated with the inhibitory functions of phloroglucinol on LPS-induced cell adhesion molecules. Furthermore, LPS-induced nuclear factor-κB (NF-κB) and tumor necrosis factor-κB (TNF-κB) release from human umbilical vein endothelial cells (HUVECs) was inhibited by phloroglucinol. Given these results, phloroglucinol could be a candidate as a therapeutic agent for various systemic inflammatory diseases.
authors: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, South Korea. baejs@knu.ac.kr
authors: Jong-Sup
authors: JS
authors: Bae
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D018836:Inflammation Mediators; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D010696:Phloroglucinol / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D019040:E-Selectin; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D010696:Phloroglucinol