title: Ionic liquid-assisted SDS-PAGE to improve human serum protein separation.
abstract: Ionic liquid (IL)-assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ILs-SDS-PAGE) was presented to improve protein separation. ILs were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (BSA/Lyz) and human serum separation. The influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. The results showed that ILs played a role in improving some protein separation, and ILs-SDS-PAGE provided higher resolution and separation efficiency than ordinary SDS-PAGE for low and middle relative molecular mass proteins in human serum. In addition, the principle of ILs-SDS-PAGE was discussed and the comparison of ILs-SDS-PAGE with ordinary SDS-PAGE and Native PAGE was made.
authors: School of Life Science, Beijing Institute of Technology, Beijing, P. R. China.
authors: Tao
authors: T
authors: Zhang
authors: Qingqing
authors: Q
authors: Gai
authors: Feng
authors: F
authors: Qu
authors: Yukui
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D007093:Imidazoles; D052578:Ionic Liquids