title: Renal immunohistochemical investigation for the differentiation of the cause of multiple trauma fatalities.
abstract: In fatalities with multiple traumatic injuries, it is important to determine the severity of trauma, the main damaged organ, and the antemortem pathophysiological condition. We examined 63 cases within 48 h of the postmortem interval, which included assaults, slips and falls and falls from heights, traffic accidents, and sharp instrumental injuries. Immunohistochemically, each kidney was stained against hemoglobin (Hb), myoglobin (Mb), superoxide dismutase (SOD), 8-hydroxy-2'-deoxyguanosine (8-OHdG), 150 kDa oxygen regulated protein (ORP150), pulmonary surfactant A (SP-A), and liver-type fatty acid binding protein (L-FABP). Bleeding or circulatory failure induced ORP150, 8-OHdG, and L-FABP in the kidney. Statistical analysis of the immunoreactivity revealed that in battered and/or abused cases, Hb could be considered a specific marker. Hb and Mb were observed in the cases with general severe trauma, such as slips and falls and falls from heights. In traffic accidents, ORP150 could reflect general circulatory failure with bleeding. SP-A was observed in the cases with severe thoracic injuries, such as lung injuries and multiple thoracic fractures. L-FABP appeared in cases with renal circulatory failure as well as renal injury. These findings suggest that immunohistochemical observation of the kidneys could be a useful tool in determining several key factors, such as the severity of injury, the specific damaged organ, and the pathological condition after injury.
authors: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan.
authors: Tomoko
authors: T
authors: Sugimura
authors: Elaine Lu
authors: EL
authors: Wang
authors: Masayuki
authors: M
authors: Kashiwagi
authors: Kenji
authors: K
authors: Hara
authors: Aya
authors: A
authors: Matsusue
authors: Brian
authors: B
authors: Waters
authors: Shin-ichi
authors: S
authors: Kubo
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005260:Female; D049429:Forensic Pathology; D018840:HSP70 Heat-Shock Proteins; D006454:Hemoglobins / Q000378:metabolism; D006470:Hemorrhage / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015601:Injury Severity Score; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009104:Multiple Trauma / Q000378:metabolism* / Q000401:mortality*; D009211:Myoglobin / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism; D012769:Shock / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D006454:Hemoglobins; D009211:Myoglobin; D011506:Proteins; D037662:Pulmonary Surfactant-Associated Protein A; C050087:oxygen-regulated proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D013482:Superoxide Dismutase; D003849:Deoxyguanosine