title: A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
abstract: To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP also reveals a large inner membrane-associated complex, which we term MitOS for mitochondrial organizing structure, comprised of Fcj1/Mitofilin, a conserved inner membrane protein, and five additional components. MitOS physically and functionally interacts with both outer and inner membrane components and localizes to extended structures that wrap around the inner membrane. We show that MitOS acts in concert with ATP synthase dimers to organize the inner membrane and promote normal mitochondrial morphology. We propose that MitOS acts as a conserved mitochondrial skeletal structure that differentiates regions of the inner membrane to establish the normal internal architecture of mitochondria.
authors: Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA.
authors: Suzanne
authors: S
authors: Hoppins
authors: Sean R
authors: SR
authors: Collins
authors: Ann
authors: A
authors: Cassidy-Stone
authors: Eric
authors: E
authors: Hummel
authors: Rachel M
authors: RM
authors: Devay
authors: Laura L
authors: LL
authors: Lackner
authors: Benedikt
authors: B
authors: Westermann
authors: Maya
authors: M
authors: Schuldiner
authors: Jonathan S
authors: JS
authors: Weissman
authors: Jodi
authors: J
authors: Nunnari
mesh_terms: D004843:Epistasis, Genetic; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics*; D051336:Mitochondrial Membranes / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D015003:Yeasts / Q000166:cytology
chemical_list: D024101:Mitochondrial Proteins; D046912:Multiprotein Complexes; D025261:Mitochondrial Proton-Translocating ATPases