title: Enhancement of carcinogenesis and fatty infiltration in the pancreas in N-nitrosobis(2-oxopropyl)amine-treated hamsters by high-fat diet.
abstract: OBJECTIVE
  Obesity is associated with increased pancreatic cancer risk, although the mechanisms have yet to be detailed. This study aimed to elucidate promotion of pancreatic cancer by obesity and hyperlipidemia.
  
  
  METHODS
  Six-week-old female Syrian golden hamsters were treated with N-nitrosobis(2-oxopropyl)amine (BOP) and after 1 week were fed a high-fat diet (HFD) or standard diet (STD) for 6 or 17 weeks.
  
  
  RESULTS
  Body weight and serum levels of lipids and leptin were significantly higher in the HFD than the STD group at 14 weeks of age. Pancreatic ductal adenocarcinomas developed only in the BOP + HFD group, with an incidence of 67% (P < 0.01) at 14 weeks of age. In addition, the multiplicity was 2-fold greater in the BOP + HFD group than in the BOP + STD group (P < 0.05) at 25 weeks of age. Pancreatic fatty infiltration was increased by BOP treatment and further enhanced by the HFD, correlating with progression of BOP-induced pancreatic ductal adenocarcinoma and up-regulated expression of adipocytokines and cell proliferation-related genes in the pancreas.
  
  
  CONCLUSIONS
  High-fat diet is shown to increase serum lipid levels and enhance fatty infiltration in the pancreas with abnormal adipocytokine production, which may accelerate and enhance pancreatic cancer.
authors: Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Tokyo, Japan.
authors: Mika
authors: M
authors: Hori
authors: Tsukasa
authors: T
authors: Kitahashi
authors: Toshio
authors: T
authors: Imai
authors: Rikako
authors: R
authors: Ishigamori
authors: Shinji
authors: S
authors: Takasu
authors: Michihiro
authors: M
authors: Mutoh
authors: Takashi
authors: T
authors: Sugimura
authors: Keiji
authors: K
authors: Wakabayashi
authors: Mami
authors: M
authors: Takahashi
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000209:etiology; D054392:Adipokines / Q000097:blood / Q000235:genetics; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D015197:Carcinogenicity Tests; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000209:etiology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D006224:Cricetinae; D059305:Diet, High-Fat; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008647:Mesocricetus; D009602:Nitrosamines / Q000633:toxicity*; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000097:blood / Q000209:etiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D054392:Adipokines; D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine