title: [Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC].
abstract: OBJECTIVE
  A new method for simultaneous determination of solasonine (1), solamargine (2) and khasianine (3) in Solanum Nigrum by reversed-phase HPLC was developed.
  
  
  METHODS
  The samples were separated at 30 degrees C on Agilent Zorbax SB C18 (4.6 mm x 150 mm, 5 microm) column with acetonitrile-water-phosphoric as mobile phase. Flow rate was 1.0 mL x min(-1) and the detection wavelength was 205 nm.
  
  
  RESULTS
  There was good linearity between the peak area and concentration at the ranges of 0.860-10.320 microg (r = 0.999 7), 0.726-8.710 microg (r = 0.999 7), 0.856-10.270 microg (r = 0.999 7) for 1, 2 and 3 respectively. The average recoveries of 1, 2 and 3 were 101.04%, 99.65%, 100.17%.
  
  
  CONCLUSIONS
  The method is rapid, simple and accurate, and it can be used for the evaluation of Solanum Nigrum L.
authors: Taizhou Polytechnic College, Taizhou 225300, China. yuanjian8101@163.com
authors: Haijian
authors: H
authors: Yuan
authors: Yigang
authors: Y
authors: Chen
authors: Baochang
authors: B
authors: Cai
authors: Xiaobin
authors: X
authors: Jia
authors: Yan
authors: Y
authors: Chen
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010840:Phytosterols / Q000737:chemistry*; D012991:Solanaceous Alkaloids / Q000737:chemistry*; D032324:Solanum nigrum / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D010840:Phytosterols; D012991:Solanaceous Alkaloids; C110213:khasianine; C026609:beta-solamarine; C026608:alpha-solamargine