title: Proteomic tissue profiling for the improvement of grading of noninvasive papillary urothelial neoplasia.
abstract: OBJECTIVE
  In 2004, a novel grading system for papillary non-invasive bladder cancer was introduced; low grade (LG) and high grade (HG) in lieu of the former G1, G2, G3. This change allowed for increased reproducibility as well as diminished interobserver variability in histopathological grading among individual pathologists. Matrix Assisted Laser Desorption/Ionization Time of Flight Imaging Mass Spectrometry (MALDI TOF IMS) was evaluated as an automatic and objective tool to assist grading of urothelial neoplasms and to facilitate accuracy.
  
  
  METHODS
  To separate G1 (LG, n=27) and G3 (HG, n=21) papillary tumors MALDI TOF IMS was performed using an appropriate algorithm. Thereafter, the automatic assignment of a separate G2 (n=31) group was completed.
  
  
  RESULTS
  G1 (LG) and G3 (HG) tumors were separated with an overall cross validation of 97.18%. G2 tumors indicated a true positive rate of 78.3% for LG and 87.5% for HG, respectively.
  
  
  CONCLUSIONS
  MALDI TOF IMS is a powerful support tool to ascertain pathological diagnosis/grading.
authors: Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany.
authors: Rena F
authors: RF
authors: Oezdemir
authors: Nadine T
authors: NT
authors: Gaisa
authors: Katharina
authors: K
authors: Lindemann-Docter
authors: Sonja
authors: S
authors: Gostek
authors: Ralf
authors: R
authors: Weiskirchen
authors: Maike
authors: M
authors: Ahrens
authors: Kristina
authors: K
authors: Schwamborn
authors: Christian
authors: C
authors: Stephan
authors: David
authors: D
authors: Pfister
authors: Axel
authors: A
authors: Heidenreich
authors: Ruth
authors: R
authors: Knuechel
authors: Corinna
authors: C
authors: Henkel
mesh_terms: D000465:Algorithms; D001331:Automation; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015233:Models, Statistical; D015588:Observer Variation; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000473:pathology*; D019459:Urothelium / Q000378:metabolism* / Q000473:pathology
chemical_list: D020543:Proteome