title: A fibroblast/macrophage co-culture model to evaluate the biocompatibility of an electrospun Dextran/PLGA scaffold and its potential to induce inflammatory responses.
abstract: Fibroblasts and macrophages are the two major types of cells responding to implanted biomaterials. They play crucial roles in inflammatory responses, host-material interactions and tissue remodeling. However, the synergistic interactions of these two cell types with biomaterials are not fully understood. In this investigation, an in vitro fibroblast/macrophage co-culture system was utilized to examine the biocompatibility and the potential to induce inflammatory responses of an electrospun Dextran/PLGA scaffold. The scaffold did not affect the morphologies, attachments, proliferations and viabilities of both the fibroblasts and macrophages, cultured separately or together. Moreover, it only activated a small subset of the macrophages implicating a low potential to induce either severe acute or chronic inflammatory response. Additionally, fibroblasts played a role in prolonging macrophage activation in the presence of the scaffolds. Using antibody arrays, IL-10, SDF-1, MIP-1 gamma and RANTES were found to be up-regulated when the cells were incubated with the scaffolds. The results of subdermal implantation of the Dextran/PLGA scaffolds confirmed its biocompatibility and low inflammatory potential.
authors: Department of Biomedical Engineering, State University of New York-Stony Brook, USA.
authors: Hui
authors: H
authors: Pan
authors: Hongliang
authors: H
authors: Jiang
authors: Sarah
authors: S
authors: Kantharia
authors: Weiliam
authors: W
authors: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D001681:Biological Assay / Q000379:methods; D018920:Coculture Techniques / Q000379:methods; D016207:Cytokines / Q000276:immunology; D003911:Dextrans / Q000009:adverse effects; D004563:Electrochemistry / Q000379:methods; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000187:drug effects* / Q000276:immunology*; D007249:Inflammation / Q000139:chemically induced* / Q000276:immunology*; D019344:Lactic Acid / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008422:Materials Testing; D051379:Mice; D011100:Polyglycolic Acid / Q000009:adverse effects; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054457:Tissue Scaffolds / Q000009:adverse effects*
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D003911:Dextrans; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid