title: Application of a bacteriophage lysin to disrupt biofilms formed by the animal pathogen Streptococcus suis.
abstract: Bacterial biofilms are crucial to the pathogenesis of many important infections and are difficult to eradicate. Streptococcus suis is an important pathogen of pigs, and here the biofilm-forming ability of 32 strains of this species was determined. Significant biofilms were completely formed by 10 of the strains after 60 h of incubation, with exopolysaccharide production in the biofilm significantly higher than that in the corresponding planktonic cultures. S. suis strain SS2-4 formed a dense biofilm, as revealed by scanning electron microscopy, and in this state exhibited increased resistance to a number of antibiotics (ampicillin, amoxicillin, ciprofloxacin, kanamycin, and rifampin) compared to that of planktonic cultures. A bacteriophage lysin, designated LySMP, was used to attack biofilms alone and in combination with antibiotics and bacteriophage. The results demonstrated that the biofilms formed by S. suis, especially strains SS2-4 and SS2-H, could be dispersed by LySMP and with >80% removal compared to a biofilm reduction by treatment with either antibiotics or bacteriophage alone of less than 20%; in addition to disruption of the biofilm structure, the S. suis cells themselves were inactivated by LySMP. The efficacy of LySMP was not dose dependent, and in combination with antibiotics, it acted synergistically to maximize dispersal of the S. suis biofilm and inactivate the released cells. These data suggest that bacteriophage lysin could form part of an effective strategy to treat S. suis infections and represents a new class of antibiofilm agents.
authors: Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors: Xiangpeng
authors: X
authors: Meng
authors: Yibo
authors: Y
authors: Shi
authors: Wenhui
authors: W
authors: Ji
authors: Xueling
authors: X
authors: Meng
authors: Jing
authors: J
authors: Zhang
authors: Hengan
authors: H
authors: Wang
authors: Chengping
authors: C
authors: Lu
authors: Jianhe
authors: J
authors: Sun
authors: Yaxian
authors: Y
authors: Yan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D024881:Drug Resistance, Bacterial; D008855:Microscopy, Electron, Scanning; D009088:Mucoproteins / Q000302:isolation & purification / Q000378:metabolism*; D017108:Streptococcus Phages / Q000201:enzymology*; D017011:Streptococcus suis / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D009088:Mucoproteins; D014764:Viral Proteins; C010393:lysin, gastropoda