title: Yeast thioredoxin-enriched extracts for mitigating the allergenicity of foods.
abstract: Thioredoxin (TRX) catalyzes the reduction of disulfide bonds in proteins via the NADPH-dependent thioredoxin reductase system. Reducing the disulfide bonds of allergenic proteins in food by TRX lowers the allergenicity. We established in this study a method to prepare TRX-enriched extracts from the edible yeast, Saccharomyces cerevisiae, on a large and practical scale, with the objective of developing TRX-containing functional foods to mitigate food allergy. Treating with the yeast TRX-enriched extracts together with NADPH and yeast thioredoxin reductase enhanced the pepsin cleavage of β-lactoglobulin and ovomucoid (OM). We also examined whether yeast TRX can mitigate the allergenicity of OM by conducting immediate allergy tests on guinea pigs. The treatment with TRX reduced the anaphylactic symptoms induced by OM in these tests. These results indicate that yeast TRX was beneficial against food allergy, raising the possibility that yeast TRX-enriched extracts can be applied to food materials for mitigating food allergy.
authors: Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd., Nagahama, Shiga, Japan. y-aoki@oyc.co.jp
authors: Yukiko
authors: Y
authors: Taketani
authors: Kimihiro
authors: K
authors: Kinugasa
authors: Shuhei
authors: S
authors: Furukawa
authors: Hajime
authors: H
authors: Nakamura
authors: Ryoko
authors: R
authors: Otsuki
authors: Hisataka
authors: H
authors: Yasuda
authors: Tuyosi
authors: T
authors: Fujita
authors: Ken
authors: K
authors: Kanzaki
authors: Hiroshi
authors: H
authors: Masutani
authors: Junji
authors: J
authors: Yodoi
mesh_terms: D000485:Allergens / Q000276:immunology* / Q000378:metabolism*; D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D055951:Functional Food / Q000633:toxicity; D005656:Fungal Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007782:Lactoglobulins / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D010059:Ovomucin / Q000276:immunology / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D020033:Protein Isoforms / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D000485:Allergens; D000906:Antibodies; D005656:Fungal Proteins; D007782:Lactoglobulins; D020033:Protein Isoforms; D010059:Ovomucin; D013879:Thioredoxins