title: Real-time monitoring of cardiovascular function in rhesus macaques infected with Zaire ebolavirus.
abstract: Nine rhesus macaques were implanted with multisensor telemetry devices and internal jugular vein catheters before being infected with Zaire ebolavirus. All animals developed viremia, fever, a hemorrhagic rash, and typical changes of Ebola hemorrhagic fever in clinical laboratory tests. Three macaques unexpectedly survived this usually lethal disease, making it possible to compare physiological parameters in lethally challenged animals and survivors. After the onset of fever, lethal illness was characterized by a decline in mean arterial blood pressure, an increase in pulse and respiratory rate, lactic acidosis, and renal failure. Survivors showed less pronounced change in these parameters. Four macaques were randomized to receive supplemental volumes of intravenous normal saline when they became hypotensive. Although those animals had less severe renal compromise, no apparent survival benefit was observed. This is the first report of continuous physiologic monitoring in filovirus-infected nonhuman primates and the first to attempt cardiovascular support with intravenous fluids.
authors: Department of Preventive Medicine, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD 20814, USA. mkortepeter@idcrp.org
authors: Mark G
authors: MG
authors: Kortepeter
authors: James V
authors: JV
authors: Lawler
authors: Anna
authors: A
authors: Honko
authors: Mike
authors: M
authors: Bray
authors: Joshua C
authors: JC
authors: Johnson
authors: Bret K
authors: BK
authors: Purcell
authors: Gene G
authors: GG
authors: Olinger
authors: Robert
authors: R
authors: Rivard
authors: Matthew J
authors: MJ
authors: Hepburn
authors: Lisa E
authors: LE
authors: Hensley
mesh_terms: D000136:Acid-Base Equilibrium; D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure; D001806:Blood Urea Nitrogen; D001831:Body Temperature; D002405:Catheterization, Central Venous; D003404:Creatinine / Q000097:blood; D029043:Ebolavirus; D004562:Electrocardiography; D005260:Female; D005440:Fluid Therapy; D019142:Hemorrhagic Fever, Ebola / Q000097:blood / Q000503:physiopathology* / Q000628:therapy; D006863:Hydrogen-Ion Concentration; D007022:Hypotension / Q000628:therapy; D007668:Kidney / Q000503:physiopathology*; D019344:Lactic Acid / Q000097:blood; D008253:Macaca mulatta; D008297:Male; D012367:RNA, Viral / Q000097:blood; D011897:Random Allocation; D012119:Respiration; D013686:Telemetry / Q000295:instrumentation
chemical_list: D012367:RNA, Viral; D019344:Lactic Acid; D003404:Creatinine