title: Characterization of p38 MAPKs from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.
abstract: p38 mitogen-activated protein kinases (MAPKs) are broadly expressed signaling molecules that involves in the regulation of cellular responsible for various extracellular stimuli. In this study, three p38 MAPK genes (Ec-p38a, p38b and p38β) were cloned from grouper, Epinephelus coioides and their characteristics were investigated in vitro. Although Ec-p38a, p38b and p38β showed high homologies to other fish p38a MPAK, p38b MAPK and p38β MAPK, respectively, they all contained the conserved structures of Thr-Gly-Tyr (TGY) motif and substrate binding site Ala-Thr-Arg-Trp (ATRW). Phylogenetic analysis indicated that Ec-p38a, p38b and p38β are more closely related to those from fish than mammals. The tissue distribution patterns of Ec-p38a, p38b and p38β were different, and Ec-p38β was up-regulated most obviously in head kidney after Singapore grouper iridovirus (SGIV) infection. Overexpression of Ec-p38β in FHM cells delayed the occurrence of CPE induced by SGIV infection. Further analysis indicated that overexpression of Ec-p38β inhibited viral gene transcription and protein synthesis, as well as SGIV induced typical apoptosis in fish cells. Taken together, our data indicated that Ec-p38β played a crucial role in regulating apoptosis and virus replication during iridovirus infection.
authors: Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
authors: Jia
authors: J
authors: Cai
authors: Youhua
authors: Y
authors: Huang
authors: Shina
authors: S
authors: Wei
authors: Xiaohong
authors: X
authors: Huang
authors: Fuzhou
authors: F
authors: Ye
authors: Jing
authors: J
authors: Fu
authors: Qiwei
authors: Q
authors: Qin
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004266:DNA Virus Infections / Q000201:enzymology / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000201:enzymology* / Q000276:immunology / Q000821:virology*; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D017994:Iridovirus; D008969:Molecular Sequence Data; D010473:Perciformes; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D014779:Virus Replication / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases