title: Japanese encephalitis virus structural and nonstructural proteins expressed in Escherichia coli induce protective immunity in mice.
abstract: Ectodomain of Japanese encephalitis virus (JEV) E protein [domains I through III (D1-3), domains I and II (D1-2) and domain III (D3)] and the nonstructural protein 1 (NS1) were expressed in Escherichia coli, and administered to BALB/c mice via the intranasal (i.n.) route. The E protein, but not the NS1, induced JEV-specific serum IgG with virus-neutralization capacity in vitro. When mice were lethally challenged with JEV, i.n. immunization with D1-3, D1-2, D3, or a mouse brain-derived formalin-inactivated JE vaccine conferred complete protection, while an 80% protection rate was observed in the NS1 immunized mice. Cytokine analysis of the cervical lymph nodes of mice i.n. immunized with D1-3 or NS1 revealed antigen-specific IL-2 and IL-17 responses, but no IFN-γ T cell response, were observed. This study demonstrates for the first time the i.n. vaccine efficacy of the E. coli-expressed recombinant JEV proteins.
authors: Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan; GenoLac BL Corporation, 1831-1 Oroku, Naha, Okinawa 901-0152, Japan.
authors: Senji
authors: S
authors: Tafuku
authors: Takeshi
authors: T
authors: Miyata
authors: Masayuki
authors: M
authors: Tadano
authors: Ryotarou
authors: R
authors: Mitsumata
authors: Hirochika
authors: H
authors: Kawakami
authors: Tetsuya
authors: T
authors: Harakuni
authors: Tomomitsu
authors: T
authors: Sewaki
authors: Takeshi
authors: T
authors: Arakawa
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000517:prevention & control*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D020381:Interleukin-17 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D020381:Interleukin-17; D007376:Interleukin-2; D022321:Japanese Encephalitis Vaccines; C100315:NS1 protein, Flavivirus; D011994:Recombinant Proteins; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins