title: The effects of acellular amniotic membrane matrix on osteogenic differentiation and ERK1/2 signaling in human dental apical papilla cells.
abstract: The amniotic membrane (AM) has been widely used in the field of tissue engineering because of the favorable biological properties for scaffolding material. However, little is known about the effects of an acellular AM matrix on the osteogenic differentiation of mesenchymal stem cells. In this study, it was found that both basement membrane side and collagenous stroma side of the acellular AM matrix were capable of providing a preferential environment for driving the osteogenic differentiation of human dental apical papilla cells (APCs) with proven stem cell characteristics. Acellular AM matrix potentiated the induction effect of osteogenic supplements (OS) such as ascorbic acid, β-glycerophosphate, and dexamethasone and enhanced the osteogenic differentiation of APCs, as seen by increased core-binding factor alpha 1 (Cbfa-1) phosphorylation, alkaline phosphatase activity, mRNA expression of osteogenic marker genes, and mineralized matrix deposition. Even in the absence of soluble OS, acellular AM matrix also could exert the substrate-induced effect on initiating APCs' differentiation. Especially, the collagenous stroma side was more effective than the basement membrane side. Moreover, the AM-induced effect was significantly inhibited by U0126, an inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) signaling. Taken together, the osteogenic differentiation promoting effect on APCs is AM-specific, which provides potential applications of acellular AM matrix in bone/tooth tissue engineering.
authors: School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors: Yi-Jane
authors: YJ
authors: Chen
authors: Min-Chun
authors: MC
authors: Chung
authors: Chung-Chen
authors: CC
authors: Jane Yao
authors: Chien-Hsun
authors: CH
authors: Huang
authors: Hao-Hueng
authors: HH
authors: Chang
authors: Jiiang-Huei
authors: JH
authors: Jeng
authors: Tai-Horng
authors: TH
authors: Young
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000650:Amnion / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003771:Dental Papilla / Q000166:cytology* / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D005786:Gene Expression Regulation; D005819:Genetic Markers; D005994:Glycerophosphates / Q000378:metabolism; D006801:Humans; D020935:MAP Kinase Signaling System; D010012:Osteogenesis; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D005819:Genetic Markers; D005994:Glycerophosphates; D012333:RNA, Messenger; C493709:RUNX2 protein, human; D003907:Dexamethasone; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid