title: Release of FITC-BSA from poly(l-lactic acid) microspheres analysis using flow cytometry.
abstract: In this investigation, biodegradable polymer poly(L-lactic acid) (PLA) microspheres were prepared by the W(1)/O/W(2) solvent evaporation method. The inner phase was aqueous solution (W(1)) that contained bovine serum albumin that was labeled with fluorescein isothiocyanate (FITC-BSA). PLA was dissolved in chloroform with emulsifier sorbitan monooleate (span 80) as the dispersed phase (O). These two solutions (W(1)/O) were emulsified by a homogenizer to form a primary emulsion. Polyvinyl alcohol (PVA) used as surfactant, was applied in the formation of microspheres (W(2)). 0.5% (w/v) PLA was stirred at 3000 rpm using a homogenizer. Microspheres with sizes of up to around 10 μm were produced. These microspheres were separated by the glycerol gradient method, and take microspheres at part of 25% glycerol gradient concentration was analyzed by flow cytometry, indicating a more homogeneous particle size distribution than that not separated. The microspheres were degraded using several enzymes, and around 40% was degraded by 72 h. This result reveals the effectiveness of drug delivery by PLA microspheres, which was evaluated by performing a drug release test and flow cytometric analysis. The FITC-BSA concentration in the supernatant increased with the experimental time. At the phagocytosis experiments, encapsulated with FITC-BSA drug of microspheres can be used by the cell, as particle size approximately 1 μm.
authors: Department of Nursing, I-Shou University, Kaohsiung 82445, Taiwan, ROC.
authors: Chih-Feng
authors: CF
authors: Kuo
authors: Nina
authors: N
authors: Tsao
authors: Hsin-Hao
authors: HH
authors: Chou
authors: Yi-Ling
authors: YL
authors: Liu
authors: Wen-Chuan
authors: WC
authors: Hsieh
mesh_terms: D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D010587:Phagocytosis; D011091:Polyesters / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D011091:Polyesters; D012710:Serum Albumin, Bovine; C033616:poly(lactide); D016650:Fluorescein-5-isothiocyanate