title: Acinetobacter baumannii multidrug-resistant strain occurrence in liver recipients with reference to other high-risk groups.
abstract: BACKGROUND
  The increasing clinical significance of Acinetobacter baumannii species is due to its ability to survive in hospital environments, its species-specific multidrug resistance, and its ability to instantly develop various drug-resistance mechanisms through antibiotic pressure.
  
  
  METHODS
  We identified 16 A baumannii strains isolated from patients presenting postoperative infections in 2010. A baumannii isolates were obtained from clinical specimens by standard microbiologic methods. As previously described, we performed polymerase chain reaction (PCR) analysis for carbapenemase-encoding genes (VIM, IMP, SPM, OXA23, OXA24, OXA51, OXA58) in Acinetobacter spp.
  
  
  RESULTS
  The double-disk synergy test phenotypic method did not detect any A baumannii strains producing metallo-beta-lactamaus cultured from swabs from all the patient groups. No products of PCR amplification with specific starters for VIM, IMP, and SPM (Sao Paulo metallo-β-lactamase) genes were found. All analyzed strains were colistin-sensitive. Among five strains from liver recipients, one was imipenem- and meropenem-resistant. Four among six strains isolated from cancer patients were resistant to imipenem and/or meropenem; 1/5 were imipenem-and meropenem-resistant; 1, meropenem-resistant and imipenem-sensitive; 1, meropenem- and imipenem-resistant; and 1 with intermediate resistance to both meropenem and imipenem among swabs cultured from patients with postoperative complication after bone fracture. Fifteen among 16 analyzed A baumannii strains had an OXA51 gene. Two among five A baumannii strains isolated in liver recipients had only an OXA51 gene; one, OXA51 and OXA24 genes; one, OXA51 and OXA23 genes.
authors: Department of Medical Microbiology, Warsaw Medical University, and Regional Hospital of Traumatology, St Anna, Warsaw, Poland. irenanets@gmail.com
authors: I
authors: I
authors: Netsvyetayeva
authors: M
authors: M
authors: Sikora
authors: M
authors: M
authors: Golas
authors: E
authors: E
authors: Swoboda-Kopec
authors: S Walter
authors: SW
authors: de Walthoffen
authors: O
authors: O
authors: Dembicka
authors: M
authors: M
authors: Fraczek
authors: A
authors: A
authors: Mlynarczyk
authors: M
authors: M
authors: Pacholczyk
authors: A
authors: A
authors: Chmura
authors: G
authors: G
authors: Mlynarczyk
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000209:etiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D050723:Fractures, Bone / Q000601:surgery; D005798:Genes, Bacterial; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects*; D009369:Neoplasms / Q000150:complications; D011183:Postoperative Complications / Q000188:drug therapy / Q000209:etiology* / Q000382:microbiology; D012307:Risk Factors; D013530:Surgical Wound Infection / Q000188:drug therapy / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase