title: New centromeric component CENP-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein.
abstract: CENP-W was originally identified as a putative oncogene, cancer-upregulated gene 2 (CUG2) that was commonly up-regulated in many cancer tissues. Recently, CENP-W has also been identified as a new centromeric component that interacts with CENP-T. As a complex with CENP-T, CENP-W plays crucial roles in assembly of the functional kinetochore complex. In this study, the subnuclear localization of CENP-W was extensively analyzed using various approaches. We found that ectopically expressed CENP-W primarily accumulated in the nucleolus and remained substantially associated with the nucleolus in stable cells. The following fractionation study also showed that CENP-W is associated with RNA as well as DNA. Moreover, a considerable amount of CENP-W was found in the nuclear mesh-like structure, nuclear matrix, possibly indicating that CENP-W participates in diverse subnuclear activities. Finally, biochemical affinity binding analysis revealed that CENP-W specifically interacts with the nucleolar phosphoprotein, nucleophosmin (B23). Depletion of cellular B23 by siRNA treatment induced a dramatic decrease of CENP-W stability and severe mislocalization during prophase. Our data proposed that B23 may function in the assembly of the kinetochore complex by interacting with CENP-W during interphase.
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Younghwa
authors: Y
authors: Chun
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Byoungwoo
authors: B
authors: Park
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Wansoo
authors: W
authors: Koh
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Sunhee
authors: S
authors: Lee
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Yeongmi
authors: Y
authors: Cheon
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Raehyung
authors: R
authors: Kim
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea.
authors: Lihua
authors: L
authors: Che
authors: Department of Microbiology and Molecular Biology, Chungnam National University, 305-764, Daejeon, Republic of Korea. Electronic address: leesoojin@cnu.ac.kr.
authors: Soojin
authors: S
authors: Lee
mesh_terms: D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D000090243:Nucleophosmin; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C534000:CENPW protein, human; D002868:Chromosomal Proteins, Non-Histone; C000716967:NPM1 protein, human; D034521:Nuclear Matrix-Associated Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D000090243:Nucleophosmin; D012313:RNA