title: Inducing-apoptotic activity of the ethanol extract of Duchesnea indica Focke on treatment of herpes simplex encephalitis.
abstract: This study explores the inducing-apoptotic activity of the ethanol extract of Duchesnea indica Focke on treatment of herpes simplex encephalitis. Cell models were employed and divided into 4 groups: normal group, virus group, Duchesnea indica group and dexamethasone group. Cytopathic effect examination was employed to detect apoptosis of PC-12 and BV-2 cells. ELISA was used to measure TNF-α, IL-1β, and Greiss method to measure NO secretion. Flow cytometry assay for caspase-3 expressions was performed. As a result, the ethanol extract of Duchesnea indica could protect the neuron cell model from impairment by virus. In the cell model of microglia stimulated by herpes simplex virus (HSV), with the ethanol extract intervention, TNF-α, IL-1β and NO levels were significantly decreased and cell death of BV-2 cells were markedly increased. The expression level of caspase-3 was notably elevated after the extract intervention. In conclusion, the ethanol extract of Duchesnea indica can reduce HSV-induced inflammatory injury on neuron due to the induction of microglia apoptosis.
authors: Department of Neurology, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
authors: X-F
authors: XF
authors: Li
authors: Y-J
authors: YJ
authors: Guo
authors: M-L
authors: ML
authors: Wang
authors: D-M
authors: DM
authors: Zhang
authors: Y-H
authors: YH
authors: Li
authors: Y-F
authors: YF
authors: Wang
authors: J-Y
authors: JY
authors: Tao
authors: S-L
authors: SL
authors: Zhang
authors: J-H
authors: JH
authors: Dong
authors: Lv-Li
authors: LL
authors: Li
authors: Lei
authors: L
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000473:pathology*; D053148:Caspase 3 / Q000096:biosynthesis; D004396:Coloring Agents; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D020803:Encephalitis, Herpes Simplex / Q000188:drug therapy* / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol; D005434:Flow Cytometry; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D016716:PC12 Cells; D010936:Plant Extracts / Q000627:therapeutic use; D031987:Potentilla / Q000737:chemistry*; D051381:Rats; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D053583:Interleukin-1beta; D010936:Plant Extracts; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000431:Ethanol; D053148:Caspase 3; C022616:thiazolyl blue