title: Potential of fluorescent metalloproteinase substrates for cancer detection.
abstract: OBJECTIVE
  MMP-2, MMP-9, their complexes and ADAM12 are detected in the urine of breast cancer patients and predict disease status. We assessed the use of FRET-based substrates in an assay to distinguish breast cancer patients from controls.
  
  
  METHODS
  Substrates with varying specificities for MMP-9 and MMP-2 and several ADAMs were screened. Flsub21 and Flsub13, substrates for ADAM12 and ADAM8 respectively, were studied.
  
  
  RESULTS
  Flsub21 and Flsub13 cleavage activities were detected in the urine of patients with invasive and metastatic breast cancers at significantly higher frequencies compared to controls. Our model predicted probabilities of 90% when both Flsub21 and Flsub13 were positive, 65% when Flsub21 alone was positive, 55% when Flsub13 alone was positive and 20% when both substrates were negative.
  
  
  CONCLUSIONS
  These data suggest the potential utility of FRET substrates to non-invasively identify invasive and/or metastatic breast cancer.
authors: Program in Vascular Biology and Department of Surgery, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
authors: Roopali
authors: R
authors: Roy
authors: David
authors: D
authors: Zurakowski
authors: Susan
authors: S
authors: Pories
authors: Marcia L
authors: ML
authors: Moss
authors: Marsha A
authors: MA
authors: Moses
mesh_terms: D014408:Biomarkers, Tumor / Q000652:urine*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000652:urine; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis* / Q000556:secondary / Q000652:urine; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000175:diagnosis* / Q000556:secondary / Q000652:urine; D016022:Case-Control Studies; D057075:Enzyme Assays; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D016015:Logistic Models; D008666:Metalloendopeptidases / Q000652:urine*; D015999:Multivariate Analysis; D009842:Oligopeptides / Q000737:chemistry*; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D009842:Oligopeptides; D008666:Metalloendopeptidases