title: Age, genetic characteristics and number of cycles are critical factors to consider for successful protection of the murine heart with postconditioning.
abstract: Postconditioning (PostC) is a recently discovered phenomenon whereby brief repetitive cycles of ischaemia with intermittent reperfusion following prolonged ischaemia elicit cardioprotection. This study investigated whether the age, genetic characteristics or number of repetitive cycles influenced the protective effect of PostC in mice. C57BL/6 floxed or non-floxed STAT-3 mice aged between 14-16 weeks (young) or 18-20 weeks (older) were perfused on a Langendorff apparatus and subjected to 35 min global ischaemia and 45 min reperfusion. PostC was elicited by either 3 (PostC-3) or 6 cycles (PostC-6) of 10 s ischaemia and 10 s reperfusion. PostC-3 and PostC-6 in both young and older non-floxed mice reduced the myocardial infarct size. In contrast, only PostC-3 reduced myocardial infarct size in young floxed mice. Neither PostC-3 nor PostC-6 reduced the infarct in older floxed mice. Our data reveal that genetic characteristics, a minute difference in age or the number of postconditioning cycles are critical factors to be considered for the successful effect of ischaemic postconditioning in a murine model. Moreover, these factors should be taken into consideration for future experimental research or clinical applications of this protective phenomenon.
authors: Hatter Cardiovascular Research Institute, University of Cape Town, Cape Town, South Africa. sarin54@gmail.com
authors: S J
authors: SJ
authors: Somers
authors: L
authors: L
authors: Lacerda
authors: L
authors: L
authors: Opie
authors: S
authors: S
authors: Lecour
mesh_terms: D000818:Animals; D057775:Ischemic Postconditioning; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D017202:Myocardial Ischemia / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse