title: Measurement of bacterial volume by transmission-through-dye imaging.
abstract: Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.
authors: Department of Biological Sciences, Kent State University, Kent, OH 44242, USA.
authors: Suzanne L
authors: SL
authors: Lababidi
authors: Mariana
authors: M
authors: Pelts
authors: Moumita
authors: M
authors: Moitra
authors: Laura G
authors: LG
authors: Leff
authors: Michael A
authors: MA
authors: Model
mesh_terms: D001419:Bacteria / Q000166:cytology*; D018914:Cells, Immobilized; D004396:Coloring Agents / Q000378:metabolism*; D008853:Microscopy / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents