title: Microscopy refocusing and dark-field imaging by using a simple LED array.
abstract: The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.
authors: Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA. gazheng@caltech.edu
authors: Guoan
authors: G
authors: Zheng
authors: Christopher
authors: C
authors: Kolner
authors: Changhuei
authors: C
authors: Yang
mesh_terms: D000465:Algorithms; D000818:Animals; D004625:Embryo, Nonmammalian / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D013215:Starfish