title: Quantitative analysis of a quaternary ammonium drug: ipratropium bromide by LC/ESI-MS(n)  in horse plasma and urine.
abstract: A quantitative method, using LC/ESI-MS(n) with a quadrupole linear ion trap mass analyzer, has been developed for the analysis of ipratropium cation in horse plasma and urine. The method applies solid-phase extraction with WCX cartridges for plasma and MM2 cartridges for urine, prior to analysis by LC/ESI-MS(n). The efficiency of extraction combined with the sensitivity and the selectivity of MS(n) allows for the quantification of ipratropium cation at picogram per milliliter levels. The analytical capabilities of the method have been successfully checked by the quantitative analysis of ipratropium cation in post-administration samples collected from horses treated by nebulization.
authors: LCH, Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370, Verrières le Buisson, France.
authors: P
authors: P
authors: Garcia
authors: A-C
authors: AC
authors: Paris
authors: A
authors: A
authors: Leufroy
authors: M-A
authors: MA
authors: Popot
authors: Y
authors: Y
authors: Bonnaire
mesh_terms: D000818:Animals; D018680:Cholinergic Antagonists / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006736:Horses / Q000097:blood* / Q000652:urine*; D009241:Ipratropium / Q000097:blood* / Q000652:urine*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D018680:Cholinergic Antagonists; D009241:Ipratropium