title: Ghrelin attenuates gastrointestinal epithelial damage induced by doxorubicin.
abstract: OBJECTIVE
  To examine the influence of ghrelin on the regenerative potential of gastrointestinal (GI) epithelium.
  
  
  METHODS
  Damage to GI epithelium was induced in mice by two intravenous injections of doxorubicin (10 and 6 mg/kg). Some of the doxorubicin-treated mice received a continuous subcutaneous infusion of ghrelin (1.25 μg/h) for 10 d via implanted mini-osmotic pumps. To label dividing stem cells in the S-phase of the cell cycle, all mice received a single intraperitoneal injection of 5'-bromo-2'-deoxyuridine (BrdU) one hour before sacrifice. The stomach along with the duodenum were then removed and processed for histological examination and immunohistochemistry using anti-BrdU antibody.
  
  
  RESULTS
  The results showed dramatic damage to the GI epithelium 3 d after administration of chemotherapy which began to recover by day 10. In ghrelin-treated mice, attenuation of GI mucosal damage was evident in the tissues examined post-chemotherapy. Immunohistochemical analysis showed an increase in the number of BrdU-labeled cells and an alteration in their distribution along the epithelial lining in response to damage by doxorubicin. In mice treated with both doxorubicin and ghrelin, the number of BrdU-labeled cells was reduced when compared with mice treated with doxorubicin alone.
  
  
  CONCLUSIONS
  The present study suggests that ghrelin enhances the regenerative potential of the GI epithelium in doxorubicin-treated mice, at least in part, by modulating cell proliferation.
authors: Department of Physiology, Faculty of Medicine and Health Sciences, UAE University, Al Ain, PO Box 17666, United Arab Emirates.
authors: Mohamed A
authors: MA
authors: Fahim
authors: Hazem
authors: H
authors: Kataya
authors: Rkia
authors: R
authors: El-Kharrag
authors: Dena Am
authors: DA
authors: Amer
authors: Basel
authors: B
authors: al-Ramadi
authors: Sherif M
authors: SM
authors: Karam
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000009:adverse effects*; D005260:Female; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000473:pathology* / Q000502:physiology; D054439:Ghrelin / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012038:Regeneration / Q000187:drug effects
chemical_list: D000903:Antibiotics, Antineoplastic; D054439:Ghrelin; D004317:Doxorubicin