title: Aberrant axial mineralization precedes spinal ankylosis: a molecular imaging study in ank/ank mice.
abstract: BACKGROUND
  The diagnosis of ankylosing spondylitis is made from a combination of clinical features and the presence of radiographic evidence that may be detected only after many years of inflammatory back pain. It is not uncommon to have a diagnosis confirmed 5 to 10 years after the initial onset of symptoms. Development of a more-sensitive molecular imaging technology to detect structural changes in the joints would lead to earlier diagnosis and quantitative tracking of ankylosis progression. Progressive ankylosis (ank/ank) mice have a loss of function in the Ank gene, which codes for a regulator of PPi transport. In this study, we used these ank/ank mutant mice to assess a noninvasive, quantitative measure of joint ankylosis with near-infrared (NIR) molecular imaging in vivo.
  
  
  METHODS
  Three age groups (8, 12, and 18 weeks) of ank/ank (15 mice) and wild-type littermates (12 +/+ mice) were assessed histologically and radiographically. Before imaging, OsteoSense 750 (bisphosphonate pamidronate) was injected i.v. Whole-body images were analyzed by using the multispectral Maestro imaging system.
  
  
  RESULTS
  OsteoSense 750 signals in the paw joints were higher in ank/ank mice in all three age groups compared with controls. In the spine, significantly higher OsteoSense 750 signals were detected early, in 8-week-old ank/ank mice compared with controls, although minimal radiographic differences were noted at this time point. The molecular imaging changes in the ank/ank spine (8 weeks) were supported by histologic changes, including calcium apatite crystals at the edge of the vertebral bodies and new syndesmophyte formation.
  
  
  CONCLUSIONS
  Changes in joint pathology of ank/ank mice, as evaluated by histologic and radiographic means, are qualitative, but only semiquantitative. In contrast, molecular imaging provides a quantitative assessment. Ankylosis in ank/ank mice developed simultaneously in distal and axial joints, contrary to the previous notion that it is a centripetal process. NIR imaging might be feasible for early disease diagnosis and for monitoring disease progression in ankylosing spondylitis.
authors: Institute of Medical Science, University of Toronto, 1 King’s College Circle, Toronto, Ontario, M5S 1A8, Canada.
authors: Facundo
authors: F
authors: Las Heras
authors: Ralph S
authors: RS
authors: DaCosta
authors: Kenneth P H
authors: KP
authors: Pritzker
authors: Nigil
authors: N
authors: Haroon
authors: George
authors: G
authors: Netchev
authors: Hing Wo
authors: HW
authors: Tsui
authors: Basil
authors: B
authors: Chiu
authors: W Mark
authors: WM
authors: Erwin
authors: Florence W L
authors: FW
authors: Tsui
authors: Robert D
authors: RD
authors: Inman
mesh_terms: D000818:Animals; D001368:Axis, Cervical Vertebra / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D002113:Calcification, Physiologic / Q000235:genetics; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control; D051379:Mice; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods*; D013167:Spondylitis, Ankylosing / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors