title: Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil.
abstract: A Gram-staining-positive, moderately halophilic bacterium, designated strain BH097(T), was isolated from solar saltern soil of Bigeum Island in south-west Korea. Cells were motile rods, producing spherical endospores at a terminal position in swollen sporangia. Strain BH097(T) was strictly aerobic, grew at pH 5.5-9.5 (optimum, pH 8.0), at 10-52 °C (optimum, 37 °C) and at salinities of 1-22% (w/v) NaCl (optimum, 7% NaCl). On the basis of 16S rRNA gene sequence analysis, strain BH097(T) was shown to belong to the genus Gracilibacillus within the phylum Firmicutes, and showed closest sequence similarity to Gracilibacillus saliphilus DSM 19802(T) (95.8%), Gracilibacillus thailandensis TP2-8(T) (95.6%), Gracilibacillus boraciitolerans DSM 17256(T) (95.5%), 'Gracilibacillus quinghaiensis' DSM 17858 (95.4%) and Gracilibacillus halophilus DSM 17856(T) (95.2%). The DNA G+C content of this novel isolate was 37.9 mol%. The major cellular fatty acids of strain BH097(T) were anteiso-C(15:0), iso-C(15:0) and C(16:0), and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol two unknown phospholipids and a glycolipid. The isoprenoid quinone was MK-7, and the peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, strain BH097(T) represents a novel species of the genus Gracilibacillus for which the name Gracilibacillus bigeumensis sp. nov. is proposed. The type strain is BH097(T) ( = KCTC 13130(T) = DSM 19028(T)).
authors: Department of Microbiology, Chungnam National University, Gung-dong 220, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors: Pankyung
authors: P
authors: Kim
authors: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Jae-Chan
authors: JC
authors: Lee
authors: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Dong-Jin
authors: DJ
authors: Park
authors: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Kee-Sun
authors: KS
authors: Shin
authors: Department of Microbiology, Chungnam National University, Gung-dong 220, Yuseong-gu, Daejeon 305-764, Republic of Korea.
authors: Jeong-Yoon
authors: JY
authors: Kim
authors: Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors: Chang-Jin
authors: CJ
authors: Kim
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012965:Sodium Chloride