title: Methods for studying Neisseria meningitidis biofilms.
abstract: Neisseria meningitidisis an organism whose environmental niche is limited to the human host. It can frequently colonize the human nasopharynx and has the ability to cause severe systemic infections. These infections can be sporadic, endemic or occur in outbreaks associated with more virulent meningococcal strains. Studies have demonstrated that the meningococcus can form biofilms both in vivo and ex vivo. In this chapter, we discuss methods to establish biofilms in the laboratory for in-depth biochemical, genetic, or microscopic studies.
authors: The Department of Microbiology, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA. michael-apicella@uiowa.edu
authors: Michael A
authors: MA
authors: Apicella
authors: Jianqiang
authors: J
authors: Shao
authors: R Brock
authors: RB
authors: Neil
mesh_terms: D018441:Biofilms; D018929:Cell Culture Techniques / Q000379:methods; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D018613:Microscopy, Confocal; D009305:Nasopharynx / Q000382:microbiology*; D009345:Neisseria meningitidis; D040901:Proteomics / Q000379:methods