title: Super-resolution 3D microscopy of live whole cells using structured illumination.
abstract: Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.
authors: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. shaol@janelia.hhmi.org
authors: Lin
authors: L
authors: Shao
authors: Peter
authors: P
authors: Kner
authors: E Hesper
authors: EH
authors: Rego
authors: Mats G L
authors: MG
authors: Gustafsson
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004331:Drosophila melanogaster / Q000166:cytology; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008870:Microtubules; D008928:Mitochondria