title: Evidence of Methylobacterium spp. and Hyphomicrobium sp. in azaspiracid toxin contaminated mussel tissues and assessment of the effect of azaspiracid on their growth.
abstract: A flagellar protein belonging to the genus Methylobacterium or Agrobacterium was previously observed by proteomics in azaspiracids (AZA) toxic mussels. Here, we report the isolation of two different Methylobacterium spp. (NTx1 and Tx1) from non-toxic and AZA toxic mussels, respectively, which when co-cultured with AZA exhibited significantly different growth responses - isolate Tx1 growth rate was enhanced, whereas growth of isolate NTx1 was adversely affected, compared to non-AZA supplemented control cultures. A Hyphomicrobium sp. (Tx2) also isolated from the toxic mussels achieved greater cell density in AZAs supplemented cultures.
authors: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, Northern Ireland, UK. jk.nzoughet@qub.ac.uk
authors: Judith K
authors: JK
authors: Nzoughet
authors: Irene R
authors: IR
authors: Grant
authors: Paulo A
authors: PA
authors: Prodöhl
authors: John T G
authors: JT
authors: Hamilton
authors: Luis M
authors: LM
authors: Botana
authors: Christopher T
authors: CT
authors: Elliott
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005517:Foodborne Diseases / Q000382:microbiology*; D020582:Hyphomicrobium / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D008387:Marine Toxins / Q000032:analysis* / Q000494:pharmacology; D020580:Methylobacterium / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D050296:Microbial Viability / Q000187:drug effects; D049880:Mytilus edulis / Q000737:chemistry / Q000382:microbiology*; D013141:Spiro Compounds / Q000032:analysis* / Q000494:pharmacology; D053719:Tandem Mass Spectrometry; D000095702:Polyether Toxins
chemical_list: D008387:Marine Toxins; D013141:Spiro Compounds; C406592:azaspiracid; D000095702:Polyether Toxins