title: Ebola Reston virus infection of pigs: clinical significance and transmission potential.
abstract: In 2008, Reston ebolavirus (REBOV) was isolated from pigs during a disease investigation in the Philippines. Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2) infections were also confirmed in affected herds and the contribution of REBOV to the disease outbreak remains uncertain. We have conducted experimental challenge studies in 5-week-old pigs, with exposure of animals to 10(6) TCID(50) of a 2008 swine isolate of REBOV via either the oronasal or subcutaneous route. Replication of virus in internal organs and viral shedding from the nasopharynx were documented in the absence of clinical signs of disease in infected pigs. These observations confirm not only that asymptomatic infection of pigs with REBOV occurs, but that animals so affected pose a transmission risk to farm, veterinary, and abattoir workers.
authors: Australian Animal Health Laboratory, Livestock Industries, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Geelong, Australia. glenn.marsh@csiro.au
authors: Glenn A
authors: GA
authors: Marsh
authors: Jessica
authors: J
authors: Haining
authors: Rachel
authors: R
authors: Robinson
authors: Adam
authors: A
authors: Foord
authors: Manabu
authors: M
authors: Yamada
authors: Jennifer A
authors: JA
authors: Barr
authors: Jean
authors: J
authors: Payne
authors: John
authors: J
authors: White
authors: Meng
authors: M
authors: Yu
authors: John
authors: J
authors: Bingham
authors: Pierre E
authors: PE
authors: Rollin
authors: Stuart T
authors: ST
authors: Nichol
authors: Lin-Fa
authors: LF
authors: Wang
authors: Deborah
authors: D
authors: Middleton
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D029043:Ebolavirus / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D019142:Hemorrhagic Fever, Ebola / Q000097:blood / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D008297:Male; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D000956:Antigens, Viral; D007074:Immunoglobulin G