title: Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones.
abstract: Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD.
authors: MRC Centre for Synaptic Plasticity, School of Biochemistry, University of Bristol, University Walk, Bristol, UK. h.i.cimarosti@reading.ac.uk
authors: Helena
authors: H
authors: Cimarosti
authors: Emi
authors: E
authors: Ashikaga
authors: Nadia
authors: N
authors: Jaafari
authors: Laura
authors: L
authors: Dearden
authors: Philip
authors: P
authors: Rubin
authors: Kevin A
authors: KA
authors: Wilkinson
authors: Jeremy M
authors: JM
authors: Henley
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D016923:Cell Death; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D009154:Mutation; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D012845:Sindbis Virus / Q000235:genetics; D058207:Sumoylation
chemical_list: D025842:SUMO-1 Protein; D049452:Green Fluorescent Proteins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C000630128:SENP1 protein, rat; D005947:Glucose; D010100:Oxygen