title: [Relationship between viral genotype and specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance].
abstract: OBJECTIVE
  To explore relationship between different HBV genotypes and peripheral blood HBV specific and nonspecific CTL of patients with cirrhotic hepatitis B and its significance.
  
  
  METHODS
  HBV genotypes were tested in 91 patients with cirrhotic hepatitis B, differences of HBV specific and nonspecific CTL between patients infected with genotype B and C were compared and its significance was explored.
  
  
  RESULTS
  In 91 cases of cirrhotic hepatitis B, 55 cases (60.44%) belong to genotype C, 35 cases (38.46%) belong to genotype B, 1 case (1.1%) belongs to mixture genotype B and C. In genotype C, 27 cases (49.09%) had positive (HLA)-A2, HBV specific CTL was 0.18% +/- 0.03%. In genotype B, 18 cases (51.43%) had positive HLA-A2, HBV specific CTL was 0.38% +/- 0.04%, higher than that in genotype C, t = 5.01, P < 0.01. Nonspecific CTL: genotype C (11.87% +/- 1.50%); genotype B (11.90% +/- 1.51%), t = 0.14, P < 0.05. HBV DNA level: genotype C (6.01 +/- 0.81) log10 copy/ml, higher than that in genotype B (5.01 +/- 0.54) log10 copy/ml, t = 5.01, P < 0.01. ALT: genotype C (251.13 +/- 131.11) U/L, higher than that in genotype B (121.25 +/- 63.21) U/L, t = 3.61, P < 0.01. TBil (45.61 +/- 15.11) micromol/L, higher than that in genotype B (28.11 +/- 6.25) micromol/L, t = 3.05, P < 0.01.
  
  
  CONCLUSIONS
  Compared with patients infected with genotype B of cirrhotic hepatitis B, HBV specific CTL of patients infected with genotype C was lower, resulting in higher level of HBV DNA and more severe damage of liver function.
authors: Wuxi Hospital for Infectious Diseases, Wuxi 214005, China. gxb188681@sina.com
authors: Xi-Bing
authors: XB
authors: Gu
authors: Xiao-Juan
authors: XJ
authors: Yang
authors: Juan-Hua
authors: JH
authors: Wang
authors: Zhong
authors: Z
authors: Hua
authors: Zhong-Hua
authors: ZH
authors: Lu
authors: Yue-Qin
authors: YQ
authors: Xu
authors: Zhi-Yuan
authors: ZY
authors: Jia
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008103:Liver Cirrhosis / Q000276:immunology* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D004279:DNA, Viral