title: Low-level diode laser therapy reduces lipopolysaccharide (LPS)-induced bone cell inflammation.
abstract: In this study, the aim is to investigate the cytologic effects of inflammatory bone cells after in vitro low-level laser therapy (LLLT). A human osteosarcoma cell line (MG63) was cultured, infected with lipopolysaccharide (LPS) and exposed to low-level laser treatment at 5 or 10 J/cm(2) using a 920 nm diode laser. MG63 cell attachment was observed under a microscope, and cell viability was quantified by mitochondrial colorimetric assay (MTT). LPS-treated MG63 cells were irradiated with LLLT, and the inflammatory markers iNOS, TNF-α and IL-1, were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The data were collected and analyzed by one-way analysis of variance (ANOVA); p < 0.05 indicated a statistically significant difference. Low-level laser treatment on MG63 cells increased their ability to attach and survive. After irradiation, the expression levels of iNOS, TNF-α and IL-1 in LPS-infected MG63 cells decreased over time (p < 0.05).
  
  
  CONCLUSIONS
  low-level diode laser treatment increased the MG63 cell proliferative ability and decreased the expression of inflammatory mediators in MG63 cells.
authors: Graduate School of Dentistry, Chung Shan Medical University Hospital, Chung Shan Medical University, 110, section 1, Chien Kuo N Road, Taichung City, 40201, Taiwan.
authors: Tsui Hsien
authors: TH
authors: Huang
authors: Yu Chuan
authors: YC
authors: Lu
authors: Chia Tze
authors: CT
authors: Kao
mesh_terms: D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D002448:Cell Adhesion / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D007375:Interleukin-1 / Q000235:genetics; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000633:toxicity; D028022:Low-Level Light Therapy / Q000379:methods; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II