title: Neurogenic and cardiomyogenic differentiation of mesenchymal stem cells isolated from minipig bone marrow.
abstract: The present study investigated the potential of minipig bone marrow-mesenchymal stem cells (BM-MSCs) to differentiate in vitro into neuron- and cardiomyocyte-like cells. Isolated BM-MSCs exhibited a fibroblast-like morphology, expressed CD29, CD44 and CD90, and differentiated into osteocytes, adipocytes and chondrocytes. Upon induction in two different neuronal specific media, most of BM-MSCs acquired the distinctive morphological features and positively stained for nestin, neurofilament-M (NF-M), neuronal nuclei (NeuN), β-tubulin, galactocerebroside (Gal-C) and glial fibrillary acidic protein (GFAP). Expression of nestin, GFAP and NF-M was further demonstrated by RT-PCR and RT-qPCR. Following cardiomyogenic induction, MSCs exhibited a stick-like morphology with extended cytoplasmic processes, and formed cluster-like structures. The expression of cardiac specific markers α-smooth muscle actin, cardiac troponin T, desmin and α-cardiac actin was positive for immunofluorescence staining, and further confirmed by RT-PCR and RT-qPCR. In conclusion, our results showed the in vitro differentiation ability of porcine BM-MSCs into neuron-like and cardiomyocyte-like cells.
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: B Mohana
authors: BM
authors: Kumar
authors: Geun-Ho
authors: GH
authors: Maeng
authors: Yeon-Mi
authors: YM
authors: Lee
authors: Tae-Ho
authors: TH
authors: Kim
authors: Jeong-Hyeon
authors: JH
authors: Lee
authors: Byeong-Gyun
authors: BG
authors: Jeon
authors: Sun-A
authors: SA
authors: Ock
authors: Jae-Gyu
authors: JG
authors: Yoo
authors: Gyu-Jin
authors: GJ
authors: Rho
mesh_terms: D017667:Adipocytes / Q000502:physiology; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002454:Cell Differentiation / Q000502:physiology*; D019902:Chondrocytes / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010011:Osteocytes / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers