title: Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster.
abstract: The bile adapted strain Bifidobacterium animalis subsp. lactis IPLA-R1 secretes a high molecular weight exopolysaccharide (HMW-EPS) when grown on the surface of agar-MRSC. This EPS is composed of L-rhamnopyranosyl, D-glucopyranosyl, D-galactopyranosyl and D-galactofuranosyl residues in the ratio of 3:1:1:1. Linkage analysis and 1D and 2D NMR spectroscopy were used to show that the EPS has a hexasaccharide repeating unit with the following structure: [See formula in text]. Treatment of the EPS with mild acid cleanly removed the terminal d-galactofuranosyl residue. The eps cluster sequenced for strain IPLA-R1 showed high genetic homology with putative eps clusters annotated in the genomes of strains from the same species. It is of note that several genes coding for rhamnose-precursors are present in the eps cluster, which could be correlated with the high percentage of rhamnose detected in its EPS repeated unit.
authors: Department of Chemical and Biological Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, United Kingdom.
authors: Shaun
authors: S
authors: Leivers
authors: Claudio
authors: C
authors: Hidalgo-Cantabrana
authors: Glenn
authors: G
authors: Robinson
authors: Abelardo
authors: A
authors: Margolles
authors: Patricia
authors: P
authors: Ruas-Madiedo
authors: Andrew P
authors: AP
authors: Laws
mesh_terms: D001644:Bifidobacterium; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial