title: Involvement of hGLD-2 in cytoplasmic polyadenylation of human p53 mRNA.
abstract: Cytoplasmic polyadenylation is a post-transcriptional mechanism regulating mRNA stability and translation. The human p53 3'-untranslated region (3'-UTR) contains two regions similar to cytoplasmic polyadenylation elements (CPEs) just upstream of the poly(A) hexanucleotide. Evaluation of the p53 CPE-like elements was performed by luciferase reporter assays, qPCR, and poly(A) assays. Herein, we report the down regulation of a luciferase reporter fused to the p53 3'-UTR, when human CPE-binding protein 1 (hCPEB1) is overexpressed. This inhibition is partially rescued when hCPEB1fused to hGLD-2 [a human cytoplasmic poly(A) polymerase] is overexpressed instead. The stability of a luciferase mRNA containing the p53 3'-UTR downstream, is decreased when hCPEB1 is overexpressed as seen by qPCR. Expression of hGLD-2 restores the mRNA stability. This is due to elongation of the poly(A) tail as seen by a PCR-based poly(A) test and in vitro poly(A) assay. Taken together, our results suggest that hCPEB1 and hGLD-2 are antagonizing factors regulating p53 mRNA stability.
authors: Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, Denmark.
authors: Jacob A
authors: JA
authors: Glahder
authors: Bodil
authors: B
authors: Norrild
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D026723:Polyadenylation; D011062:Polynucleotide Adenylyltransferase; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; C490386:CPEB1 protein, human; D012333:RNA, Messenger; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D039221:mRNA Cleavage and Polyadenylation Factors; D011062:Polynucleotide Adenylyltransferase; C550174:TENT2 protein, human