title: Neonatal exposure to single doses of estradiol or testosterone programs ovarian follicular development-modified hypothalamic neurotransmitters and causes polycystic ovary during adulthood in the rat.
abstract: OBJECTIVE
  To investigate the hormones participating in early follicular development and hypothalamic neurotransmitters in rats during adulthood.
  
  
  METHODS
  Experimental basic study.
  
  
  METHODS
  University animal laboratory.
  
  
  METHODS
  Twenty-three neonatal rats injected with single subcutaneous injection of estradiol valerate (EV), testosterone propionate (TP), or dihydrotestosterone (DHT) and killed by decapitation at 60 days of age.
  
  
  METHODS
  Measurements of neurotransmitter in ventromedial hypothalamus-arcuate nucleus (VMH-AN) and ovarian morphometry in the adult rat.
  
  
  METHODS
  Noradrenaline (NA), dopamine (DA), serotonin (5-HT), glutamic acid (Glu), and gamma-aminobutyric acid (GABA) content by high performance liquid chromatography medial basal hypothalamus and ovarian morphology.
  
  
  RESULTS
  EV exposure increased 5-HT, DA, NA, and Glu and decreased GABA levels in the VMH-AN. Exposure to TP increased Glu and decreased 5-HT in the VMH-AN. Neonatal EV and TP decreased the number of primordial follicles but EV increased the atresia of antral follicles and TP decreased it. Neonatal exposure to DHT did not cause morphologic changes in the adult ovary.
  
  
  CONCLUSIONS
  Neonatal exposure to EV activated the reproductive hypothalamus and permanently modified ovarian follicular development. TP exposure had some similar effects as EV at the hypothalamus, and it modified ovarian development mimicking the effects of EV. This last effect could be through TP conversion to estradiol because DHT, a nonaromatizable androgen, did not modify follicular development.
authors: Laboratory of Neurobiochemistry, Faculty of Chemistry and Pharmaceutical Sciences, Universidad de Chile, Independencia, Santiago, Chile.
authors: Ramón
authors: R
authors: Sotomayor-Zárate
authors: Michelle
authors: M
authors: Tiszavari
authors: Gonzalo
authors: G
authors: Cruz
authors: Hernán E
authors: HE
authors: Lara
mesh_terms: D000367:Age Factors; D000375:Aging / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007028:Hypothalamic Hormones / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000187:drug effects; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*; D014697:Ventromedial Hypothalamic Nucleus / Q000187:drug effects
chemical_list: D007028:Hypothalamic Hormones; D018377:Neurotransmitter Agents; D013739:Testosterone; D004958:Estradiol