title: [Effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase expression during myocardial ischemia-reperfusion in rats].
abstract: To investigate the effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase (GST) expression during myocardial ischemia-reperfusion in rats.
  
  
  
  Sprague-Dawley male rats were randomly divided into 4 groups (n= 10 in each): Group S (sham operation group), Group IR (ischemia/reperfusion group), Group H (IR+ NaHS 0.05 mg/kg iv, 24 h before ischemia) and Groups D receiving IR+NaHS 24 h before ischemia and 5-hydroxydecanoate (5-HD)15 min before ischemia. Animals in groups IR, H and D were subjected to ischemia by 30 min of coronary artery occlusion followed by 2 h of reperfusion. At the end of the reperfusion, myocardial infarct size (IS) was examined. Glutathione S-transferase (GST) was measured by Western blotting. The myocardial ultrastructures were observed under the electron microscopy.
  
  
  
  The IS was significantly smaller in Group H than that in Group IR [(25.40 ± 3.54)% compared with (38.27 ± 5.64)%, P<0.05]. The GST expression in myocardium was significantly higher in Group H than that in Group IR. Microscopic examination showed less myocardial damage in Group H than in Group IR. The protective effects of delayed preconditioning by hydrogen sulfide was prevented by 5-HD pre-treatment.
  
  
  
  The hydrogen sulfide-induced delayed preconditioning attenuates myocardial IR injury possibly through up-regulating glutathione S-transferase expression in rats.
authors: Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011,China.
authors: Ke
authors: K
authors: Ran
authors: Zheng-guo
authors: ZG
authors: Tang
authors: Li-ping
authors: LP
authors: Ding
authors: Shuang-feng
authors: SF
authors: Li
authors: Ye-tian
authors: YT
authors: Chang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005982:Glutathione Transferase / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000008:administration & dosage* / Q000627:therapeutic use; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000201:enzymology* / Q000473:pathology / Q000628:therapy; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005982:Glutathione Transferase; D006862:Hydrogen Sulfide