title: Cytochrome CYP141: a new target for direct detection of Mycobacterium tuberculosis from clinical specimens.
abstract: Cytochrome P450 CYP141 is an intermediary metabolic and respiratory protein that interferes with oxidation reduction in Mycobacterium tuberculosis. This conserved protein has also been debated as a hypothetical target for therapeutics. We used the sequences of CYP141 gene to develop a PCR for rapid detection of Mycobacterium tuberculosis from respiratory specimens. The sensitivity of this PCR for culture positive-smear positive and culture positive-smear negative samples were 92% and 62.5%, respectively. The overall sensitivity and specificity of this PCR was 85.7% and 97.8%. As compared with other studies, it appears that the CYP141 gene is a good target for direct detection of M. tuberculosis from respiratory specimens.
authors: Tehran University of Medical Sciences, Department of Microbiology, School of Medicine, Tehran, Iran.
authors: Davood
authors: D
authors: Darban-Sarokhalil
authors: Abbas Ali Imani
authors: AA
authors: Fooladi
authors: Zakaria
authors: Z
authors: Bameri
authors: Mohammad Javad
authors: MJ
authors: Nasiri
authors: Mohammad Mehdi
authors: MM
authors: Feizabadi
mesh_terms: D001431:Bacteriological Techniques; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012137:Respiratory System / Q000382:microbiology; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D003577:Cytochrome P-450 Enzyme System