title: Microsatellite markers for Aspidosperma polyneuron (Apocynaceae), an endangered tropical tree species.
abstract: OBJECTIVE
  This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species.
  
  
  RESULTS
  Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi-Guaçu river basins in São Paulo State in the southeastern region of Brazil. Twenty-five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (H(e) = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured.
  
  
  CONCLUSIONS
  These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron.
authors: Programa de Pós-graduação em Biologia Comparada, Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, 14040-901 Ribeirão Preto, São Paulo, Brazil.
authors: Ronai
authors: R
authors: Ferreira-Ramos
authors: Mariza
authors: M
authors: Monteiro
authors: Maria I
authors: MI
authors: Zucchi
authors: José B
authors: JB
authors: Pinheiro
authors: Carlos A
authors: CA
authors: Martinez
authors: Moacyr A
authors: MA
authors: Mestriner
authors: Ana Lilia
authors: AL
authors: Alzate-Marin
mesh_terms: D030009:Aspidosperma / Q000145:classification / Q000235:genetics*; D001938:Brazil; D018744:DNA, Plant / Q000032:analysis*; D056727:Endangered Species; D051456:Gene Flow; D014644:Genetic Variation; D018895:Microsatellite Repeats; D013045:Species Specificity; D014197:Trees / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant