title: Effects of methoxsalen from Poncirus trifoliata on acetylcholinesterase and trimethyltin-induced learning and memory impairment.
abstract: Previously, we identified methoxsalen (8-methoxy-2',3',6,7-furocoumarin) as the bioactive compound probably responsible for acetylcholinesterase (AchE) inhibition achieved by feeding crude extract of Poncirus trifoliate. To confirm the activity of methoxsalen, Institute of Cancer Research (ICR) mice were fed a control or a methoxsalen-supplemented diet for 4 weeks, and then learning and memory enhancing effects with respect to trimethyltin (TMT)-induced neurotoxicity were evaluated. The brain tissues of ICR mice were dissected after completion of the behavioral tests for biochemical analysis. Methoxsalen effectively reversed TMT-induced memory impairment on both Y-maze and passive avoidance tests. Brain AchE activity was inhibited by the oral consumption of all concentrations of methoxsalen. Moreover, the level of oxidative stress was significantly ameliorated in the groups on methodsalen containing diets. This is the first in vivo study conducted with methoxsalen in the field of AD research, and it indicates that further investigation of methoxsalen is warranted.
authors: Department of Food and Biotechnology, Korea University, Seoul, Republic of Korea.
authors: Jae Kyeom
authors: JK
authors: Kim
authors: Soo Jung
authors: SJ
authors: Choi
authors: Hyeri
authors: H
authors: Bae
authors: Cho Rong
authors: CR
authors: Kim
authors: Hong-Yon
authors: HY
authors: Cho
authors: Young Jun
authors: YJ
authors: Kim
authors: Seung Taik
authors: ST
authors: Lim
authors: Chang-Ju
authors: CJ
authors: Kim
authors: Hye Kyung
authors: HK
authors: Kim
authors: Sabrina
authors: S
authors: Peterson
authors: Dong Hoon
authors: DH
authors: Shin
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D004032:Diet; D007858:Learning / Q000187:drug effects*; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008730:Methoxsalen / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D016716:PC12 Cells; D032104:Poncirus / Q000737:chemistry*; D051381:Rats; D014298:Trimethyltin Compounds / Q000633:toxicity*
chemical_list: D002800:Cholinesterase Inhibitors; D014298:Trimethyltin Compounds; C046488:trimethyltin; D000110:Acetylcholinesterase; D008730:Methoxsalen