title: MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.
abstract: We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNAmanipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
authors: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA. kv134369@bcm.edu
authors: Koen J T
authors: KJ
authors: Venken
authors: Karen L
authors: KL
authors: Schulze
authors: Nele A
authors: NA
authors: Haelterman
authors: Hongling
authors: H
authors: Pan
authors: Yuchun
authors: Y
authors: He
authors: Martha
authors: M
authors: Evans-Holm
authors: Joseph W
authors: JW
authors: Carlson
authors: Robert W
authors: RW
authors: Levis
authors: Allan C
authors: AC
authors: Spradling
authors: Roger A
authors: RA
authors: Hoskins
authors: Hugo J
authors: HJ
authors: Bellen
mesh_terms: D000818:Animals; D057005:Bioengineering; D004251:DNA Transposable Elements / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation; D007438:Introns; D016254:Mutagenesis, Insertional; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D011993:Recombinant Fusion Proteins