title: TMT labelling for the quantitative analysis of adaptive responses in the meningococcal proteome.
abstract: In addition to standard gel-based proteomic approaches, gel-free approaches using isobaric label reagents, such as Tandem Mass Tags (TMT), provide a straightforward method for studying adaptations in microbial proteomes to changing environmental conditions. This approach does not have the known difficulties of 2-D gel electrophoresis with proteins of extreme biochemical properties. The workflow described here was designed to study adaptive responses in bacteria and has been applied to study the response of meningococci to iron limitation. The supplemental use of western blotting allows the confirmation of certain changes in protein abundance identified within the TMT study.
authors: Proteome Sciences R&D, Frankfurt am Main, Germany.
authors: Karsten
authors: K
authors: Kuhn
authors: Christian
authors: C
authors: Baumann
authors: Jan
authors: J
authors: Tommassen
authors: Thorsten
authors: T
authors: Prinz
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000345:Affinity Labels; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D000090463:Iron Deficiencies; D009345:Neisseria meningitidis / Q000235:genetics*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000345:Affinity Labels