title: Biomarkers in the early period of acute myocardial infarction in rat serum and protective effects of Shexiang Baoxin Pill using a metabolomic method.
abstract: BACKGROUND
  To identify the biomarkers in early period of acute myocardial infarction (AMI) in rat serum and reveal the effective mechanism of a Traditional Chinese Medicine (TCM) named Shexiang Baoxin Pill (SBP).
  
  
  METHODS
  A metabolomic approach using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed.
  
  
  RESULTS
  Fourteen biomarkers in the early period of acute myocardial infarction (AMI) in rat serum were identified. These biomarkers include 5-methylcytosine, cystathionine ketimine, 2-oxoadipic acid, thymidine, epinephrine, homocystine, uric acid, 12(S)-hydroperoxyeicosatetraenoic acid (12s-HPETE), 11-dehydrocorticosterone, 12(S)-hydroxyeicosatetraenoic acid (12s-HETE), deoxycorticosterone, corticosterone, aldosterone and cortisol. Through pathway analysis of these biomarkers, inflammation, hypertrophy and oxidative injury were considered the most relevant pathological changes in early period of AMI.
  
  
  CONCLUSIONS
  Identification of AMI biomarkers not only supplied a systematic view of the progression of AMI in the early period but also provided the theoretical basis for the prevention or treatment of AMI. The results demonstrated that SBP pretreatment could offer protective effects for AMI through regulating the pathway of steroid hormone biosynthesis.
authors: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors: Peng
authors: P
authors: Jiang
authors: Weixing
authors: W
authors: Dai
authors: Shikai
authors: S
authors: Yan
authors: Zhongliang
authors: Z
authors: Chen
authors: Ruilin
authors: R
authors: Xu
authors: Jianmi
authors: J
authors: Ding
authors: Li
authors: L
authors: Xiang
authors: Shuping
authors: S
authors: Wang
authors: Runhui
authors: R
authors: Liu
authors: Weidong
authors: W
authors: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D009203:Myocardial Infarction / Q000097:blood* / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C526712:shexiang baoxin