title: No evidence of association between 8q24 and susceptibility to nonsyndromic cleft lip with or without palate in Japanese population.
abstract: OBJECTIVE
  Recent genome-wide association studies identified susceptibility loci for nonsyndromic cleft lip with or without cleft palate (NSCL±P) on 8q24.21, 10q25.3, 13q31.1, 15q13.3, 17q22, and 18q22 in populations of European origin. The purpose of this study was to determine, using DNA samples, whether 8q24.21 was a susceptibility locus for the development of NSCL±P in Japanese patients.
  
  
  METHODS
  We used DNA from 167 Japanese NSCL±P patients (45 cleft lip without cleft palate and 122 cleft lip with cleft palate patients) and 190 Japanese unaffected control individuals. We performed an association study using 13 single nucleotide polymorphisms (SNPs) selected on the 8q24.21 locus. Genotyping of each SNP was carried out by direct sequencing of genomic DNA. Additionally, a haplotype block was constructed using the selected SNPs.
  
  
  RESULTS
  The 13 selected SNPs were successfully genotyped in 357 individuals. The p values obtained were not low enough to indicate a significant association between the haplotypes and the development of NSCL±P in this population.
  
  
  CONCLUSIONS
  Our results suggest that the 8q24.21 locus is not associated with susceptibility to NSCL±P in Japanese patients and provide further evidence that ethnicity is a strong factor in determining susceptibility loci, albeit using a limited number of samples. Further studies are needed to identify regions involved in the development of NSCL±P in the Japanese population.
authors: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan.
authors: Masanori
authors: M
authors: Hikida
authors: Masayoshi
authors: M
authors: Tsuda
authors: Akira
authors: A
authors: Watanabe
authors: Akira
authors: A
authors: Kinoshita
authors: Sadanori
authors: S
authors: Akita
authors: Akiyoshi
authors: A
authors: Hirano
authors: Takeshi
authors: T
authors: Uchiyama
authors: Koh-ichiro
authors: K
authors: Yoshiura
mesh_terms: D016022:Case-Control Studies; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007564:Japan; D008297:Male; D020641:Polymorphism, Single Nucleotide