title: Lipase Activity among Bacteria Isolated from Amazonian Soils.
abstract: The objective of this study was to select lipase-producing bacteria collected from different counties of the Amazon region. Of the 440 bacteria strains, 181 were selected for the lipase assay in qualitative tests at Petri dishes, being 75 (41%) lipase positive. The enzymatic index was determined during fifteen days at different temperatures (30°, 35°, 40°, and 45°C). The highest lipase activity was observed within 72 hours at 30°C. Twelve bacteria strains presented an index equal to or greater than the standard used like reference, demonstrating the potential of microbial resource. After the bioassay in Petri dishes, the selected bacteria strains were analyzed in quantitative tests on p-nitrophenyl palmitate (p-NPP). A group of the strains was selected for other phases of study with the use in oleaginous substrates of the Amazonian flora, aiming for the application in processes like oil biotransformation.
authors: Biochemistry and Molecular Biology Coordination, Amazon Biotechnology Center (CBA), Avnue Gov. Danilo Areosa, 690 Distrito Industrial, 69075351 Manaus, AM, Brazil.
authors: André Luis
authors: AL
authors: Willerding
authors: Luiz Antonio
authors: LA
authors: de Oliveira
authors: Francisco Wesen
authors: FW
authors: Moreira
authors: Mariana Gomes
authors: MG
authors: Germano
authors: Aloísio Freitas
authors: AF
authors: Chagas