title: Expression of dual angiogenic/neurogenic growth factors in human primary brain tumors.
abstract: Brain tumors, benign or malignant, are characterized by a very high degree of vascularization. Recent accumulating evidence suggests that during development the neuronal wiring follows the same routes as the vasculature and that these two systems may share some of the same factors for guidance. Thus, expression of dual angiogenic/neurogenic growth factors was evaluated by in situ hybridization in human primary brain tumors of three different types, i.e., astrocytomas, oligodendrogliomas, and ependymomas, of increasing grades, in relation with the grade and type of the tumor. For this evaluation we selected vascular endothelial growth factor (VEGF-A) and its receptors VEGF-R1 and VEGF-R2 and the neuropilins 1 and 2 (NRP-1 and NRP-2), which have proangiogenic properties, platelet-derived growth factor (PDGF) receptor-beta (PDGF-Rβ), which is required for the functional maturation of blood vessels, the ephrins and their Eph receptors, angiotensinogen (AGT) and thrombospondin-2 (TSP-2), which have potential antiangiogenic properties, and netrin-1 (Net-1), which regulates vascular architecture. We show that the expression of the VEGF-NRP system, PDGF-Rβ, TSP-2, AGT, and Net-1 are differentially regulated, either increased or decreased, in relation with the type and grade of the tumor, whereas regulation of the ephrinB system does not seem to be relevant in these human brain tumors.
authors: INSERM U833, Collège-de-France, 11 place Marcelin Berthelot, Paris, France.
authors: Maud
authors: M
authors: Clemessy
authors: Robert C
authors: RC
authors: Janzer
authors: Benoìt
authors: B
authors: Lhermitte
authors: Jean-Marie
authors: JM
authors: Gasc
authors: Lucienne
authors: L
authors: Juillerat-Jeanneret
mesh_terms: D042501:Angiogenic Proteins / Q000235:genetics*; D000808:Angiotensinogen / Q000235:genetics; D001932:Brain Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D060787:Neoplasm Grading; D039942:Neuropilin-1 / Q000235:genetics; D039943:Neuropilin-2 / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics; D012189:Retrospective Studies; D019699:Thrombospondins / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics; D000099262:Thrombospondin 2
chemical_list: D042501:Angiogenic Proteins; D000808:Angiotensinogen; D036341:Intercellular Signaling Peptides and Proteins; D039942:Neuropilin-1; D039943:Neuropilin-2; D015513:Oncogene Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D020797:Receptor, Platelet-Derived Growth Factor beta; D019699:Thrombospondins; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2; D000099262:Thrombospondin 2; C534931:AGT protein, human; C099763:NET1 protein, human; C467484:VEGFA protein, human