title: Characterization of mouse clonal mesenchymal stem cell lines established by subfractionation culturing method.
abstract: OBJECTIVE
  To characterize single-cell-derived mouse clonal mesenchymal stem cells (mcMSCs) established with bone marrow samples from three different mouse strains.
  
  
  METHODS
  We established mcMSC lines using subfractionation culturing method from bone marrow samples obtained from long bones. These lines were characterized by measuring cell growth, cell surface epitopes, differentiation potential, lineage-specific gene expression and T-cell suppression capability. Nonclonal MSCs isolated by the conventional gradient centrifugation method were used as controls.
  
  
  RESULTS
  All mcMSC lines showed typical nonclonal MSC-like spindle shape morphology. Lines differed in optimal growth density requirement. Cell surface epitope profiles of these mcMSC lines were similar to those of nonclonal MSCs. However, some lines exhibited different expression levels in a few epitopes, such as CD44 and CD105. Differentiation assays showed that 90% of the mcMSC lines were capable of differentiating into adipogenic and/or chondrogenic lineages, but only 20% showed osteogenic lineage differentiation. T-cell suppression analysis showed that 75% of the lines exhibited T-cell suppression capability.
  
  
  CONCLUSIONS
  mcMSC lines have similar cell morphology and cell growth rate but exhibit variations in their cell surface epitopes, differentiation potential, lineage-specific gene expression and T-cell suppression capability.
authors: Myung-Shin Jeon, Tac-Ghee Yi, Hyun-Ja Lim, Sun-Hwa Moon, Moon-Hee Lee, Joon-Soon Kang, Chul-Soo Kim, Dae-Hyun Lee, Sun U Song, Clinical Research Center, School of Medicine, Inha University, 7-206, 3-Ga, Shinheung-Dong, Chung-Gu, Inchon 400-711, South Korea.
authors: Myung-Shin
authors: MS
authors: Jeon
authors: Tac-Ghee
authors: TG
authors: Yi
authors: Hyun-Ja
authors: HJ
authors: Lim
authors: Sun-Hwa
authors: SH
authors: Moon
authors: Moon-Hee
authors: MH
authors: Lee
authors: Joon-Soon
authors: JS
authors: Kang
authors: Chul-Soo
authors: CS
authors: Kim
authors: Dae-Hyun
authors: DH
authors: Lee
authors: Sun U
authors: SU
authors: Song