title: Conformational stability of recombinant manganese superoxide dismutase from the filamentous fungus Trichoderma reesei.
abstract: Superoxide dismutases (SODs; EC 1.15.1.1) are part of the antioxidant system of aerobic organisms and are used as a defense against oxidative injury caused by reactive oxygen species (ROS). The cloning and sequencing of the 788-bp genomic DNA from Trichoderma reesei strain QM9414 (anamorph of Hypocrea jecorina) revealed an open reading frame encoding a protein of 212 amino acid residues, with 65-90% similarity to manganese superoxide dismutase from other filamentous fungi. The TrMnSOD was purified and shown to be stable from 20 to 90°C for 1h at pH from 8 to 11.5, while maintaining its biological activity.
authors: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, São Paulo, Brazil.
authors: Felipe S
authors: FS
authors: Chambergo
authors: Estela Y
authors: EY
authors: Valencia
authors: José Ribamar
authors: JR
authors: Ferreira-Júnior
authors: César M
authors: CM
authors: Camilo
authors: Patricia T
authors: PT
authors: Campana
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*; D013696:Temperature; D014242:Trichoderma / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D013482:Superoxide Dismutase