title: Deeper in the human cornea proteome using nanoLC-Orbitrap MS/MS: An improvement for future studies on cornea homeostasis and pathophysiology.
abstract: The cornea is a transparent, avascular, and highly specialized connective tissue that provides the majority of light refraction in the optical system of the eye. The human cornea is composed of several layers interacting in a complex manner and possessing specific functions, like eye protection and optical clearness. Only few proteomic studies of mammalian cornea have been performed leading to the identification of less than 200 proteins in human corneas. The present study explores the proteome of the intact normal human cornea using a shot-gun nanoLC-MS/MS strategy and an LTQ Orbitrap mass spectrometer. A total of 2070 distinct corneal proteins were identified from five human cornea samples, which represents a 14-fold improvement in the number of proteins identified so far for human cornea. This enlarged dataset of human corneal proteins represents a valuable reference library for further studies on cornea homeostasis and pathophysiology. Network and gene ontology analyses were used to determine biological pathways specific of the human cornea. They allowed the identification of subnetworks of putative importance for corneal diseases, like a redox regulation and oxidative stress network constituted of aldehyde and alcohol dehydrogenases, most of them being described for the first time in human cornea.
authors: INSERM U563, Université Toulouse III Paul Sabatier, Toulouse, F-31300, France.
authors: Stéphane D
authors: SD
authors: Galiacy
authors: Carine
authors: C
authors: Froment
authors: Emmanuelle
authors: E
authors: Mouton-Barbosa
authors: Angélique
authors: A
authors: Erraud
authors: Karima
authors: K
authors: Chaoui
authors: Laurence
authors: L
authors: Desjardins
authors: Bernard
authors: B
authors: Monsarrat
authors: François
authors: F
authors: Malecaze
authors: Odile
authors: O
authors: Burlet-Schiltz
mesh_terms: D003316:Corneal Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019573:Epithelium, Corneal / Q000737:chemistry / Q000378:metabolism*; D005136:Eye Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D005136:Eye Proteins; D020543:Proteome