title: Biomolecular filters for improved separation of output signals in enzyme logic systems applied to biomedical analysis.
abstract: Biomolecular logic systems processing biochemical input signals and producing "digital" outputs in the form of YES/NO were developed for analysis of physiological conditions characteristic of liver injury, soft tissue injury, and abdominal trauma. Injury biomarkers were used as input signals for activating the logic systems. Their normal physiological concentrations were defined as logic-0 level, while their pathologically elevated concentrations were defined as logic-1 values. Since the input concentrations applied as logic 0 and 1 values were not sufficiently different, the output signals being at low and high values (0, 1 outputs) were separated with a short gap making their discrimination difficult. Coupled enzymatic reactions functioning as a biomolecular signal processing system with a built-in filter property were developed. The filter process involves a partial back-conversion of the optical-output-signal-yielding product, but only at its low concentrations, thus allowing the proper discrimination between 0 and 1 output values.
authors: Jan
authors: J
authors: Halámek
authors: Jian
authors: J
authors: Zhou
authors: Lenka
authors: L
authors: Halámková
authors: Vera
authors: V
authors: Bocharova
authors: Vladimir
authors: V
authors: Privman
authors: Joseph
authors: J
authors: Wang
authors: Evgeny
authors: E
authors: Katz
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism*; D000818:Animals; D055162:Biocatalysis; D035843:Biomedical Research; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006321:Heart; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology; D008128:Logic; D013552:Swine
chemical_list: D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D000410:Alanine Transaminase