title: Pterocarpan profiles for soybean leaves at different growth stages and investigation of their glycosidase inhibitions.
abstract: Soybean leaves are eaten as seasonal edible greens in Korea. Analysis of the ethyl acetate extract of these leaves showed that it exhibited potent and selective neuraminidase inhibition, which began at the R3 stage and peaked at R7. Ten pterocarpans, including the new 6a-hydroxypterocarpan 10, were isolated from soybean leaves and their inhibition activities tested against a range of glycosidases. The relationship between structure and enzyme inhibition was investigated: 6a-hydroxypterocarpans exhibited much higher inhibition against neuraminidase (IC(50) = 2.4-89.4 μM) than α-glucosidase (IC(50) = 90.4- >100 μM). Glyceollin VII (7) displayed 40-fold greater activity (IC(50) = 2.4 μM) against neuraminidase than α-glucosidase (IC(50) = 90.4 μM). On the other hand, coumestanes (1-3) were good α-glucosidase inhibitors (IC(50) = 6.0-42.6 μM). In kinetic analysis, the most potent neuraminidase inhibitors (5-10) were noncompetitive. HPLC analysis indicated that most pterocarpan synthesis began from the R3 stage, and a rapid change of pterocarpan concentrations was observed between the R4 and R7 stages.
authors: Division of Applied Life Science (BK21 Program), IALS, Graduate School of Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Heung Joo
authors: HJ
authors: Yuk
authors: Marcus J
authors: MJ
authors: Curtis-Long
authors: Hyung Won
authors: HW
authors: Ryu
authors: Ki Chang
authors: KC
authors: Jang
authors: Woo Duck
authors: WD
authors: Seo
authors: Jun Young
authors: JY
authors: Kim
authors: Kyu Young
authors: KY
authors: Kang
authors: Ki Hun
authors: KH
authors: Park
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D036343:Pterocarpans / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology*; D013025:Glycine max; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D036343:Pterocarpans; D006026:Glycoside Hydrolases; D009439:Neuraminidase