title: Isolation of laccase gene from Bacillus subtilis and analysis of its physicochemical properties.
abstract: The present study reports the cloning and sequencing of lac2 from Bacillus subtilis. The gene is composed of 1542 bp and encodes a 514-amino acid protein. The gene has 86% homology with a published laccase with GeneID 936023. The lac2 gene was deposited in GenBank as a new nucleotide sequence. This new sequence was cloned into the multiple cloning site of pPIC9K to generate pPIC9K-lac2, which was then transformed into Pichia pastoris GS115 via electroporation. The recombinant GS115 (pPIC9K-lac2) was grown initially in BMGY medium and transferred to BMMY to induce gene expression for 48 h. The recombinant Lac2 protein shows laccase activity with α-naphthol and guaiacol as substrates. The optimal pH is between 3.2 and 4.7, and the optimal temperature is 25°C for enzyme reaction.
authors: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China.
authors: Sui-Shan
authors: SS
authors: Yang
authors: Zhen-Wang
authors: ZW
authors: Liu
authors: Xiao-Ping
authors: XP
authors: Yi
authors: Ai-Lian
authors: AL
authors: Zhang
authors: Tian-Yuan
authors: TY
authors: Zhang
authors: Jin-Xian
authors: JX
authors: Luo
authors: Ze-Hua
authors: ZH
authors: Zhang
authors: Jin-Cheng
authors: JC
authors: Shen
authors: Hui-Xiang
authors: HX
authors: Yin
authors: Li-Ping
authors: LP
authors: Chen
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D018274:Electroporation; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D042845:Laccase