title: Antioxidant, xanthine oxidase and lipoxygenase inhibitory activities and phenolics of Bauhinia rufescens Lam. (Caesalpiniaceae).
abstract: An aqueous acetone extract of the stem with the leaves of Bauhinia rufescens and its fractions were analysed for their antioxidant and enzyme-inhibitory activities, as well as their phytochemical composition. For measurement of the antioxidant activities, the 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis(3-ethylbenzoline-6-sulphonate) and the ferric-reducing methods were used. The results indicated that the aqueous acetone, its ethyl acetate and n-butanol fractions possessed considerable antioxidant activity. Further, the xanthine oxidase and lipoxygenase inhibitory assays showed that the n-butanol fraction possessed compounds that can inhibit both these enzymes. In the phytochemical analysis, the ethyl acetate and the n-butanol fractions of the aqueous acetone extract were screened by HPLC-MS for their phenolic content. The results indicated the presence of hyperoside, isoquercitrin, rutin quercetin, quercitrin, p-coumaric and ferulic acids in the non-hydrolysed fractions. In the hydrolysed fractions, kaempferol, p-coumaric and ferulic acids were identified.
authors: Laboratoire de Biochimie et de Chimie Appliquées-LABIOCA, UFR-SVT, Université de Ouagadougou, Ouagadougou, Burkina Faso. mcompaore_3@yahoo.fr
authors: M
authors: M
authors: Compaoré
authors: C E
authors: CE
authors: Lamien
authors: A
authors: A
authors: Lamien-Meda
authors: L
authors: L
authors: Vlase
authors: M
authors: M
authors: Kiendrebeogo
authors: C
authors: C
authors: Ionescu
authors: O G
authors: OG
authors: Nacoulma
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D036481:Bauhinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D013058:Mass Spectrometry; D010636:Phenols / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D016859:Lipoxygenase Inhibitors; D010636:Phenols; D014969:Xanthine Oxidase