title: Actinokineospora soli sp. nov., a thermotolerant actinomycete isolated from soil, and emended description of the genus Actinokineospora.
abstract: A strain of thermotolerant actinomycete, designated YIM 75948(T), was isolated from a soil sample in Yunnan province, China. The strain grew at 25-55 °C (optimum 37 °C). The substrate mycelium and aerial mycelium produced on Czapek's agar were both pale yellow to white. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were mannose, ribose, glucose, galactose and arabinose. The major fatty acids were iso-C(15:0), iso-C(16:0) and C(16:0) and the predominant respiratory quinone was MK-9(H(4))(.) The polar lipids consisted of phosphatidylethanolamine, phosphatidylethanolamine with hydroxy fatty acids, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. The genomic DNA G+C content was 73.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 75948(T) belonged in the genus Actinokineospora and that its closest relative among recognized species was Actinokineospora fastidiosa DSM 43855(T) (97.6% sequence similarity). The mean level of DNA-DNA relatedness between the novel strain and A. fastidiosa DSM 43855(T) was, however, only 47.8%. Based on the phenotypic, chemotaxonomic and phylogenetic data and the results of the DNA-DNA hybridizations, strain YIM 75948(T) represents a novel species of the genus Actinokineospora for which the name Actinokineospora soli sp. nov. is proposed. The type strain is YIM 75948(T) ( =JCM 17695(T) =DSM 45613(T)). The description of the genus Actinokineospora is emended to reflect the fact that the genomic DNA G+C contents of A. fastidiosa DSM 43855(T) and the type strain of Actinokineospora soli sp. nov. recorded in the present study fell above the range given in previous descriptions of this genus.
authors: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Xia
authors: X
authors: Tang
authors: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Yu
authors: Y
authors: Zhou
authors: Guangdong Provincial Microbial Culture Collection and Application Key Laboratory, Guangdong Institute of Microbiology, Guangzhou 510070, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Jing
authors: J
authors: Zhang
authors: Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.
authors: Hong
authors: H
authors: Ming
authors: College of Life Sciences, Henan Normal University, Xinxiang 453007, PR China.
authors: Guo-Xing
authors: GX
authors: Nie
authors: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Ling-Ling
authors: LL
authors: Yang
authors: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Shu-Kun
authors: SK
authors: Tang
authors: Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xingjiang Institue of Ecology and Geogrpahy, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors: Wen-Jun
authors: WJ
authors: Li
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012987:Soil