title: Backbone and sidechain methyl Ile (δ1), Leu and Val chemical shift assignments of RDE-4 (1-243), an RNA interference initiation protein in C. elegans.
abstract: The RNAi pathway of several organisms requires presence of double stranded RNA binding proteins for functioning of Dicer in gene regulation. In C. elegans, a double stranded RNA binding protein, RDE-4 (385 aa, 44 kDa) recognizes long exogenous dsRNA and initiates the RNAi pathway. We have achieved complete backbone and stereospecific methyl sidechain Ile (δ1), Leu and Val chemical shifts of first 243 amino acids of RDE-4, namely RDE-4ΔC.
authors: Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad 500007, India.
authors: Sai Chaitanya
authors: SC
authors: Chiliveri
authors: Sonu
authors: S
authors: Kumar
authors: Udaya Kiran
authors: UK
authors: Marelli
authors: Mandar V
authors: MV
authors: Deshmukh
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D007532:Isoleucine / Q000737:chemistry; D007930:Leucine / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000737:chemistry*; D014633:Valine / Q000737:chemistry
chemical_list: D000596:Amino Acids; D029742:Caenorhabditis elegans Proteins; C461645:RDE-4 protein, C elegans; D016601:RNA-Binding Proteins; D007532:Isoleucine; D007930:Leucine; D014633:Valine