title: Salvianolic acid B suppresses IFN-γ-induced JAK/STAT1 activation in endothelial cells.
abstract: BACKGROUND
  Dysfunction of the endothelium contributes to pathological conditions of the arterial wall including atherosclerosis as a result of immunological and/or inflammatory responses. Salvianolic acid B (Sal B), a pure and active compound extracted from the Chinese herb Salvia miltiorrhizae (SM) was characterized for its anti-inflammatory and anti-oxidant properties on vascular system.
  
  
  RESULTS
  Sal B pretreatment significantly inhibited the IFN-γ-induced phosphorylations of JAK2 (Tyr 1007/1008) and STAT1 (Tyr701 and Ser727). Consistently, IFN-γ-induced STAT1 downstream targets CXC chemokines' IP-10, Mig, and I-TAC were suppressed by Sal B pretreatment. Sal B inhibited promoter activities of IP-10 and the secretion of IP-10 protein. The monocyte adhesion to IFN-γ-treated ECs was observed to be reduced after Sal B pretreatment. ECs treated with Sal B alone also increased the expression of PIAS1 and SOCS1 which may also contribute to its inhibitory effect on JAK-STAT1 signaling pathways.
  
  
  CONCLUSIONS
  The anti-inflammatory properties of Sal B on IFN-γ-induced JAK-STAT1 activation were demonstrated in the present study which provides a molecular basis for possible therapeutic usage on vascular disorders.
authors: Department of Cardiology, New Taipei City Hospital, New Taipei City, Taiwan.
authors: Shih Chung
authors: SC
authors: Chen
authors: Yun Lian
authors: YL
authors: Lin
authors: Bin
authors: B
authors: Huang
authors: Danny Ling
authors: DL
authors: Wang
authors: Jing Jy
authors: JJ
authors: Cheng
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D002417:Cattle; D004347:Drug Interactions; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D053613:Janus Kinase 1 / Q000378:metabolism*; D019169:Jurkat Cells; D010766:Phosphorylation; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D014162:Transfection; D053630:Depsides
chemical_list: D001572:Benzofurans; D007371:Interferon-gamma; D053613:Janus Kinase 1; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; C076944:salvianolic acid B; D053630:Depsides; C507904:JAK1 protein, human