title: Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea.
abstract: A recombinant protein-based enzyme-linked immunosorbent assay (RP ELISA) exists for the detection of antibodies to foot-and-mouth disease virus (FMDV) type A. In this study, the efficacy of the RP ELISA was compared to that of other current tests by examining sera collected in the field during an FMD type A outbreak in South Korea in 2010. The RP ELISA detected early antibodies to FMDV with the same sensitivity as the liquid-phase blocking ELISA (LPB ELISA), identifying FMD farm outbreaks correctly on a herd basis. In addition, the two assays exhibited a high correlation coefficient (γ(2)=0.83) when testing thirty seven sera from one outbreak farm exhibiting various antibody titers. The sensitivity and specificity of the RP ELISA relative to the LPB ELISA were 84% and 97%, respectively, and excellent agreement (kappa=0.82) was observed between the two tests. Taken together, the RP ELISA should be a useful alternative to the LPB ELISA for the detection of early antibodies to FMDV type A during an outbreak.
authors: National Veterinary Research and Quarantine Service, Anyang, Gyeonggi, 430-757, South Korea. koyoungjoon@korea.kr
authors: Young-Joon
authors: YJ
authors: Ko
authors: Hyang-Sim
authors: HS
authors: Lee
authors: Jong-Hyeon
authors: JH
authors: Park
authors: Kwang-Nyeong
authors: KN
authors: Lee
authors: Su-Mi
authors: SM
authors: Kim
authors: In-Soo
authors: IS
authors: Cho
authors: Hoo-Don
authors: HD
authors: Joo
authors: Sang-Gi
authors: SG
authors: Paik
authors: David J
authors: DJ
authors: Paton
authors: Satya
authors: S
authors: Parida
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000453:epidemiology; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D011994:Recombinant Proteins; D056910:Republic of Korea / Q000453:epidemiology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins