title: Winogradskyella aquimaris sp. nov., isolated from seawater.
abstract: A Gram-negative, non-flagellated, motile by gliding, aerobic rod, designated DPG-24(T), was isolated from seawater of Geoje Island in the South Sea, Korea. Strain DPG-24(T) grew optimally at 30-37 °C, at pH 7.0-7.5 and with 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DPG-24(T) belonged to the genus Winogradskyella and clustered with Winogradskyella poriferorum UST030701-295(T) and Winogradskyella exilis 022-2-26(T). Strain DPG-24(T) exhibited 97.6 and 95.8% 16S rRNA gene sequence similarities with W. poriferorum UST030701-295(T) and W. exilis 022-2-26(T), respectively, and 92.4-95.7% with other members of the genus Winogradskyella. Strain DPG-24(T) contained MK-6 as the predominant menaquinone and iso-C(15:1) G, iso-C(15:0) and iso-C(17:0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The DNA G+C content was 36.7 mol%. DNA-DNA relatedness between the isolate and W. poriferorum JCM 12885(T) was 10.8%. The differential phenotypic properties and phylogenetic and genetic distinctiveness enabled strain DPG-24(T) to be differentiated from recognized members of the genus Winogradskyella. On the basis of the data presented, strain DPG-24(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella aquimaris sp. nov. is proposed; the type strain is DPG-24(T) ( = KCTC 23502(T)  = CCUG 60798(T)).
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Soo-Young
authors: SY
authors: Lee
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Sooyeon
authors: S
authors: Park
authors: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
authors: Tae-Kwang
authors: TK
authors: Oh
authors: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors: Jung-Hoon
authors: JH
authors: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6