title: Controlled green tea polyphenols release from electrospun PCL/MWCNTs composite nanofibers.
abstract: Poly(ɛ-caprolactone)/multi-walled carbon nanotubes (PCL/MWCNTs) composite nanofibers with various content of green tea polyphenols (GTP) were successfully fabricated via an electrospinning technology to maintain the chemical structural stability of GTP. The non-covalent interaction between MWCNTs and GTP was measured by UV-vis spectrophotometer and FT-IR. The topographical features of the nanofibers were characterized by scanning electron microscopy (SEM). The dispersibility of MWCNTs and the distribution of GTP in nanofibers were observed by transmission electron microscopy (TEM) and laser scanning confocal microscope (LSCM), respectively. In vitro degradation was also characterized in terms of the morphological change and the mass loss of the nanofiber meshes. In vitro GTP release behavior was investigated in phosphate-buffered solution (PBS) at 37°C. Alamar blue assays were performed to estimate the cytotoxicity of the nanofibers with normal osteoblast cells and the antiproliferative effects to A549 and Hep G2 tumor cells. The results exhibited that the GTP-loaded composite nanofibers possessed a significant inhibition effect to tumor cells. Therefore, GTP, as a multifunctional drug, encapsulated into polymer composite nanofibers, must have broad application prospects in cancer therapy.
authors: School of Materials Science and Engineering, Key Laboratory of Advanced Technologies of Material, Minister of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China.
authors: Shijun
authors: S
authors: Shao
authors: Long
authors: L
authors: Li
authors: Guang
authors: G
authors: Yang
authors: Jingrong
authors: J
authors: Li
authors: Chao
authors: C
authors: Luo
authors: Tao
authors: T
authors: Gong
authors: Shaobing
authors: S
authors: Zhou
mesh_terms: D000327:Adsorption; D000818:Animals; D028241:Camellia sinensis; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts; D011091:Polyesters / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry* / Q000633:toxicity; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003692:Delayed-Action Preparations; D037742:Nanotubes, Carbon; D011091:Polyesters; D059808:Polyphenols; C016240:polycaprolactone