title: A novel system for identification of inhibitors of rift valley Fever virus replication.
abstract: Rift Valley fever virus (RVFV) is a human and livestock pathogen endemic to sub-Saharan Africa. We have developed a T7-dependent system for the efficient production of RVFV-like particles (RVF-VLPs) based on the virulent ZH-501 strain of RVFV. The RVF-VLPs are capable of performing a single round of infection, allowing for the study of viral replication, assembly, and infectivity. We demonstrate that these RVF-VLPs are antigenically indistinguishable from authentic RVFV and respond similarly to a wide array of known and previously unknown chemical inhibitors. This system should be useful for screening for small molecule inhibitors of RVFV replication.
authors: Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA.
authors: Mary E
authors: ME
authors: Piper
authors: Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA.|Department of Epidemiology, University of Michigan, Ann Arbor, MI, USA.
authors: Sonja R
authors: SR
authors: Gerrard