title: Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1.
abstract: BACKGROUND
  Scavenger receptors are generally expressed in macrophages and vascular endothelial cells and some scavenger receptors are thought to contribute to the development of atherosclerosis.
  
  
  METHODS
  We cloned the cDNA of a zebrafish CL-P1 (collectin placenta 1) and performed a knockdown study using its antisense morpholino oligonucleotides (MO).
  
  
  RESULTS
  Zebrafish CL-P1 (zCL-P1) is 51% identical to human CL-P1 in its amino acid sequence. Microbes and OxLDL bound to zCL-P1 cDNA transfected cells. zCL-P1 mRNA expression gradually increased after 6hours post-fertilization (hpf), reached its highest level at 24hpf, and then decreased, which is similar to the gene expression pattern of Tie-2. The knockdown of zCL-P1 led to an increase in the number of zebrafish embryos with severe morphological abnormalities such as short body lengths and defects in the dorsal aorta at 48hpf. Simultaneous injection of both MO and synthetic zCL-P1 or zVEGF mRNA rescued the abnormal phenotype.
  
  
  CONCLUSIONS
  In vivo knockdown study shows that zCL-P1 is implicated in vasculogenesis and those of our in vitro study support its role as a scavenger receptor.
  
  
  CONCLUSIONS
  These results suggest that zCL-P1 might be essential for vasculogenesis during the early embryonic phase in bone fish.
authors: Department of Microbiology and Immunochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510, Japan.
authors: Mitsuko
authors: M
authors: Fukuda
authors: Katsuki
authors: K
authors: Ohtani
authors: Seong-Jae
authors: SJ
authors: Jang
authors: Takayuki
authors: T
authors: Yoshizaki
authors: Ken-Ichiro
authors: K
authors: Mori
authors: Wataru
authors: W
authors: Motomura
authors: Itsuro
authors: I
authors: Yoshida
authors: Yasuhiko
authors: Y
authors: Suzuki
authors: Yutaka
authors: Y
authors: Kohgo
authors: Nobutaka
authors: N
authors: Wakamiya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051116:Receptors, Scavenger / Q000235:genetics* / Q000502:physiology; D015027:Zebrafish
chemical_list: D018076:DNA, Complementary; D051116:Receptors, Scavenger