title: Determination of phenformin hydrochloride using molecular imprinting technology coupled with flow-injection chemiluminescence.
abstract: A novel method was developed using molecular imprinting technology (MIT) coupled with flow-injection chemiluminescence (FI-CL) for highly sensitive detection of phenformin hydrochloride (PH). The phenformin imprinted polymer was synthesized with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. Newly synthesized molecularly imprinted polymer (MIP) particles were packed into a column as a selective recognition element for determination of PH. A CL method for the determination of PH was developed based on the CL reaction of PH with N-bromosuccinimide sensitized by eosin Y in basic media. The optimization of detection conditions was investigated. The CL intensity responded linearly to the concentration of PH in the range 0.09-2.0 µg/mL, with a correlation coefficient of 0.9920. The detection limit was 0.031 µg/mL. The relative standard deviation for the determination of 1.0 µg/mL PH solution was 1.0% (n = 11). The method was applied to the determination of PH in urine samples, with satisfactory results.
authors: School of Chemical and Biological Science and Engineering, Yantai University, People's Republic of China.
authors: Zhenbo
authors: Z
authors: Liu
authors: Fengyan
authors: F
authors: Jia
authors: Wenwen
authors: W
authors: Wang
authors: Cuixia
authors: C
authors: Wang
authors: Yongming
authors: Y
authors: Liu
mesh_terms: D000327:Adsorption; D000328:Adult; D005260:Female; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005984:Glutethimide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000652:urine*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D007004:Hypoglycemic Agents; D011108:Polymers; C005440:phenglutarimide; D005984:Glutethimide