title: Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system.
abstract: BACKGROUND
  The magnetic albumin nanosphere (MAN), encapsulating maghemite nanoparticles, was designed as a magnetic drug delivery system (MDDS) able to perform a variety of biomedical applications. It is noteworthy that MAN was efficient in treating Ehrlich's tumors by the magnetohyperthermia procedure.
  
  
  METHODS
  In this study, several nanotoxicity tests were systematically carried out in mice from 30 minutes until 30 days after MAN injection to investigate their biocompatibility status. Cytometry analysis, viability tests, micronucleus assay, and histological analysis were performed.
  
  
  RESULTS
  Cytometry analysis and viability tests revealed MAN promotes only slight and temporary alterations in the frequency of both leukocyte populations and viable peritoneal cells, respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, interestingly, revealed the presence of MAN clusters in the central nervous system (CNS).
  
  
  CONCLUSIONS
  The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.
authors: Instituto de Ciências Biológicas, Universidade de Brasília, 70910-900, Brasília-DF, Brazil.
authors: Luciana
authors: L
authors: Estevanato
authors: Débora
authors: D
authors: Cintra
authors: Nayara
authors: N
authors: Baldini
authors: Flávia
authors: F
authors: Portilho
authors: Luzirlane
authors: L
authors: Barbosa
authors: Olímpia
authors: O
authors: Martins
authors: Bruno
authors: B
authors: Lacava
authors: Ana Luisa
authors: AL
authors: Miranda-Vilela
authors: Antônio Cláudio
authors: AC
authors: Tedesco
authors: Sônia
authors: S
authors: Báo
authors: Paulo C
authors: PC
authors: Morais
authors: Zulmira G M
authors: ZG
authors: Lacava
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D001923:Brain Chemistry / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006651:Histocytochemistry; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000633:toxicity*; D008422:Materials Testing; D051379:Mice; D015162:Micronucleus Tests; D054118:Nanospheres / Q000633:toxicity; D012710:Serum Albumin, Bovine / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine