title: Enantioselectivity of monolithic silica stationary phases immobilized with different concentrations cellulose tris (3,5-dimethylphenylcarbamate), analyzed with different mobile phases in capillary electrochromatography.
abstract: The 3,5-dimethylphenylcarbamate derivatives of cellulose bearing 3-(triethoxysilyl)propyl residues were immobilized in a capillary format onto a monolithic silica support by intermolecular polycondensation of the triethoxysilyl groups. The resulting columns were used for chiral separations using capillary electrochromatography. The effects of the synthesizing solvent, the selector coating procedure, the chiral selector concentration onto the silica monolith and the mobile phase pH value, on the separation of enantiomers were studied. The column-to-column reproducibility and stability also were evaluated. A test set of 14 chiral substances, including acidic, neutral, bifunctional and basic compounds, was used to investigate the effects of the factors mentioned above. Twelve pairs of enantiomers showed enantioselectivity at some of the different conditions tested. The column-to-column repeatability was satisfactory, and the prepared columns were stable under the adopted analysis conditions.
authors: Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel-VUB, Center for Pharmaceutical Research, Brussels, Belgium.
authors: Chunguang
authors: C
authors: Lv
authors: Yueqi
authors: Y
authors: Liu
authors: Debby
authors: D
authors: Mangelings
authors: Yvan
authors: Y
authors: Vander Heyden
mesh_terms: D000081:Acetamides / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018021:Lithium Chloride / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D048448:Phenylcarbamates / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D000081:Acetamides; D000097:Acetonitriles; D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D012822:Silicon Dioxide; D002482:Cellulose; D018021:Lithium Chloride; C013959:dimethylacetamide; C032159:acetonitrile