title: Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes.
abstract: We have used EM and biochemistry to characterize the structure of NuA4, an essential yeast histone acetyltransferase (HAT) complex conserved throughout eukaryotes, and we have determined the interaction of NuA4 with the nucleosome core particle (NCP). The ATM-related Tra1 subunit, which is shared with the SAGA coactivator complex, forms a large domain joined to a second region that accommodates the catalytic subcomplex Piccolo and other NuA4 subunits. EM analysis of a NuA4-NCP complex shows the NCP bound at the periphery of NuA4. EM characterization of Piccolo and Piccolo-NCP provided further information about subunit organization and confirmed that histone acetylation requires minimal contact with the NCP. A small conserved region at the N terminus of Piccolo subunit enhancer of Polycomb-like 1 (Epl1) is essential for NCP interaction, whereas the subunit yeast homolog of mammalian Ing1 2 (Yng2) apparently positions Piccolo for efficient acetylation of histone H4 or histone H2A tails. Taken together, these results provide an understanding of the NuA4 subunit organization and the NuA4-NCP interactions.
authors: Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA.
authors: Johnathan R
authors: JR
authors: Chittuluru
authors: Yuriy
authors: Y
authors: Chaban
authors: Julie
authors: J
authors: Monnet-Saksouk
authors: Michael J
authors: MJ
authors: Carrozza
authors: Vasileia
authors: V
authors: Sapountzi
authors: William
authors: W
authors: Selleck
authors: Jiehuan
authors: J
authors: Huang
authors: Rhea T
authors: RT
authors: Utley
authors: Myriam
authors: M
authors: Cramet
authors: Stephane
authors: S
authors: Allard
authors: Gang
authors: G
authors: Cai
authors: Jerry L
authors: JL
authors: Workman
authors: Michael G
authors: MG
authors: Fried
authors: Song
authors: S
authors: Tan
authors: Jacques
authors: J
authors: Côté
authors: Francisco J
authors: FJ
authors: Asturias
mesh_terms: D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000378:metabolism; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D046912:Multiprotein Complexes; D009707:Nucleosomes; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D051548:Histone Acetyltransferases; C504740:NuA4 protein, S cerevisiae