title: Kibdelosporangium phytohabitans sp. nov., a novel endophytic actinomycete isolated from oil-seed plant Jatropha curcas L. containing 1-aminocyclopropane-1-carboxylic acid deaminase.
abstract: A novel actinomycete, designated strain KLBMP 1111(T), was isolated from the root of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Strain KLBMP 1111(T) formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Kibdelosporangium, with the highest similarity to Kibdelosporangium aridum subsp. aridum DSM 43828(T) (98.8%), K. aridum subsp. largum DSM 44150(T) (98.1%) and Kibdelosporangium philippinense DSM 44226(T) (98.1%). The organism produced sporangium-like structures, the typical morphological characteristic of the genus Kibdelosporangium. The chemotaxonomic properties of this strain were also consistent with those of the genus Kibdelosporangium: the peptidoglycan contained meso-diaminopimelic acid; the predominant menaquinone was MK-9(H(4)); phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol and an unknown phospholipid; iso-C(16:0), C(16:0), anteiso-C(15:0) and iso-C(15:0) as the predominant cellular fatty acids and the G+C content was 67.2 mol%. DNA-DNA hybridization values between strain KLBMP 1111(T) and the three Kibdelosporangium species were less than 50%. This strain had the ability to produce a siderophore, utilized 1-aminocyclopropane-1-carboxylic acid (ACC) as sole source of nitrogen and possessed ACC deaminase enzyme. Based on genotypic and phenotypic data, strain KLBMP 1111(T) represents a novel species in the genus Kibdelosporangium. We propose the name Kibdelosporangium phytohabitans sp. nov. for this species. The type strain is the strain KLBMP 1111(T) (=KCTC 19775(T) = CCTCC AA 2010001(T)).
authors: School of Life Science, The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, People's Republic of China.
authors: Ke
authors: K
authors: Xing
authors: Guang-Kai
authors: GK
authors: Bian
authors: Sheng
authors: S
authors: Qin
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Bo
authors: B
authors: Yuan
authors: Yue-Ji
authors: YJ
authors: Zhang
authors: Wen-Jun
authors: WJ
authors: Li
authors: Ji-Hong
authors: JH
authors: Jiang
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D000598:Amino Acids, Cyclic / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D019755:Carbon-Carbon Lyases / Q000235:genetics / Q000378:metabolism*; D002681:China; D060026:Endophytes / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D005227:Fatty Acids / Q000378:metabolism; D031286:Jatropha / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology
chemical_list: D000598:Amino Acids, Cyclic; D001426:Bacterial Proteins; D005227:Fatty Acids; C023863:1-aminocyclopropane-1-carboxylic acid; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases