title: Immunoexpression of B7-H3 in endometrial cancer: relation to tumor T-cell infiltration and prognosis.
abstract: OBJECTIVE
  B7-H3, a member of the B7 family of immune regulatory ligands regulates T cell-mediated peripheral immune response. The purpose of this study was to correlate the expression of B7-H3 and number of lymphocytes in patients with endometrial cancer.
  
  
  METHODS
  A total of 107 patients with primary endometrial carcinoma (type I/endometrioid, n=81; type II, n=18) and endometrial hyperplasia (n=8) were investigated. Expression of B7-H3 in endometrial hyperplasia, endometrial carcinoma, and the endothelium of tumor-associated vasculature was assessed using immunohistochemistry from paraffin-embedded tissue blocks. Detection of CD8-positive tumor-infiltrating lymphocytes (TIL) and CD8-positive tumor-associated lymphocytes (TAL) was correlated with the expression of B7-H3.
  
  
  RESULTS
  Patients with high grade tumors and patients with type II carcinomas expressed significantly more B7-H3 than low grade and endometrioid tumors (p=<0.0001 and p=0.0001, respectively). The expression of B7-H3 in the endothelium of identified vasculature in the tumor specimens showed similar results with strong relation to high grade tumors (p=0.001) and type II carcinomas (p=0.004). We found a significant correlation between B7-H3 expression on cancer cells and tumor T-cell infiltration (TIL) (p=0.017). In a univariate survival analysis, overexpression of B7-H3 in tumor cells was associated with shortened overall survival (p=0.005).
  
  
  CONCLUSIONS
  B7-H3 is overexpressed on cancer cells and in the endothelium of tumor-associated vasculature in high grade tumors (G3) and type II carcinomas. B7-H3 expression on cancer cells is correlated with the number of T cells infiltrating the tumor. Endometrium tumor development and progression may be associated with downregulation of T-cell-mediated antitumor immunity through B7-H3.
authors: Department of Obstetrics and Gynecology, Landesklinikum Thermenregion Moedling/Vienna, Austria. dr.andreas.brunner@utanet.at
authors: Andreas
authors: A
authors: Brunner
authors: Susanne
authors: S
authors: Hinterholzer
authors: Paul
authors: P
authors: Riss
authors: Georg
authors: G
authors: Heinze
authors: Hermann
authors: H
authors: Brustmann
mesh_terms: D000368:Aged; D060887:B7 Antigens / Q000096:biosynthesis* / Q000276:immunology*; D001808:Blood Vessels / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018269:Carcinoma, Endometrioid / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D004714:Endometrial Hyperplasia / Q000276:immunology / Q000473:pathology; D016889:Endometrial Neoplasms / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D060887:B7 Antigens; C423237:CD276 protein, human