title: Mechanism of JmjC-containing protein Hairless in the regulation of vitamin D receptor function.
abstract: The JmjC-domain-containing protein Hairless (HR) and the vitamin D receptor (VDR) play a critical role in the maintenance of hair growth. Mutations in HR or VDR cause alopecia in humans and mice. Here we show that HR interacts with VDR and induces VDR relocalization in the nuclei. HR associates and colocalizes with nuclear receptor co-repressor (N-CoR) which is localized to subnuclear structures termed matrix-associated deacetylase (MAD) bodies. It is found that the HR mutants (C622G, N970S, D1012N, V1136D), associated with alopecia universalis congenita (AUC) or atrichia with papular lesions (APL), exhibit an abnormal subcellular distribution in addition to the impaired co-repressor activity with VDR. Studies on deletion mutants of HR indicate that the JmjC domain contributes to the co-repressor activity of HR. Our work provides new clues and evidence for the understanding on the role of HR in hair growth.
authors: Department of Biochemistry and Molecular Biology, Chinese Academy of Medical Sciences, China.
authors: Yang
authors: Y
authors: Mi
authors: Ye
authors: Y
authors: Zhang
authors: Yu-Fei
authors: YF
authors: Shen
mesh_terms: D019943:Amino Acid Substitution; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D056970:Co-Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D056971:Nuclear Receptor Co-Repressor 1 / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D018167:Receptors, Calcitriol / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D056970:Co-Repressor Proteins; C490267:HR protein, human; C539127:NCOR1 protein, human; D056971:Nuclear Receptor Co-Repressor 1; D018167:Receptors, Calcitriol; D011994:Recombinant Proteins; D014157:Transcription Factors; D049410:Luciferases, Renilla