title: Two genes, rif15 and rif16, of the rifamycin biosynthetic gene cluster in Amycolatopsis mediterranei likely encode a transketolase and a P450 monooxygenase, respectively, both essential for the conversion of rifamycin SV into B.
abstract: Amycolatopsis mediterranei produces an important antibiotic rifamycin, the biosynthesis of which involves many unusual modifications. Previous work suggested a putative P450 enzyme encoded by rif16 within the rifamycin biosynthetic gene cluster (rif) was required for the conversion of the intermediate rifamycin SV into the end product rifamycin B. In this study, we genetically proved that a putative transketolase encoded by rif15 is another essential enzyme for this conversion. Expression of merely rif15 and rif16 in a rif cluster null mutant of A. mediterranei U32 was able to convert rifamycin SV into B. However, this Rif15- and Rif16-mediated conversion was only detected in intact cells of A. meidterranei, but not in Streptomyce coelicolor or Mycobacterium smegmatis, suggesting that yet-characterized gene(s) in A. mediterranei other than those encoded by the rif cluster should be involved in this process.
authors: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
authors: Hua
authors: H
authors: Yuan
authors: Wei
authors: W
authors: Zhao
authors: Yi
authors: Y
authors: Zhong
authors: Jin
authors: J
authors: Wang
authors: Zhongjun
authors: Z
authors: Qin
authors: Xiaoming
authors: X
authors: Ding
authors: Guo-Ping
authors: GP
authors: Zhao
mesh_terms: D000191:Actinomycetaceae / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D012294:Rifamycins / Q000096:biosynthesis*; D014174:Transketolase / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012294:Rifamycins; C033974:rifamycin B; C023808:rifamycin SV; D006899:Mixed Function Oxygenases; D014174:Transketolase