title: Purification of human respiratory syncytial virus fusion glycoprotein.
abstract: Human respiratory syncytial virus (RSV) fusion glycoprotein (F) elicits neutralizing antibodies to RSV and has therefore attracted much attention as a suitable candidate antigen in the development of gene-based vaccines against RSV infections. However, a major obstacle in vaccine development has been the problem of antigen purification. To address this problem, we have developed a new method that combines sucrose gradient ultracentrifugation and a two-step chromatographic process, to purify RSV F from RSV particles propagated in HEp-2 cells. Analysis of the fractions produced using this method showed recovery of a functional homodimer with a molecular weight of 140 kDa, and 54% preservation of the original F.
authors: College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, China.
authors: Yanpeng
authors: Y
authors: Zheng
authors: College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, China.
authors: Yaning
authors: Y
authors: Tang
authors: College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, China.
authors: Yuanhui
authors: Y
authors: Fu
authors: College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, China. Electronic address: jshhe@bjtu.edu.cn.
authors: Jinsheng
authors: J
authors: He
authors: Department of Immunology, Anhui Medical University, Hefei, China.
authors: Xiaobo
authors: X
authors: Wang
authors: College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing, China; Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing 100052, China.
authors: Tao
authors: T
authors: Hong
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry / Q000302:isolation & purification*; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D056945:Hep G2 Cells; D006801:Humans; D022261:Respiratory Syncytial Virus Vaccines; D018113:Respiratory Syncytial Virus, Human / Q000737:chemistry*; D014461:Ultracentrifugation; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014771:Virion / Q000737:chemistry
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D022261:Respiratory Syncytial Virus Vaccines; D014760:Viral Fusion Proteins