title: Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor.
abstract: Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33°C, a higher temperature of 44°C and lower temperatures of 22°C and 18°C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30°C, 0.62 at 44°C, and 1.29 at 18°C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.
authors: Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, Tempe, AZ 85287-5701, USA.
authors: Jie
authors: J
authors: Sheng
authors: Hyun Woo
authors: HW
authors: Kim
authors: Jonathan P
authors: JP
authors: Badalamenti
authors: Chao
authors: C
authors: Zhou
authors: Swathi
authors: S
authors: Sridharakrishnan
authors: Rosa
authors: R
authors: Krajmalnik-Brown
authors: Bruce E
authors: BE
authors: Rittmann
authors: Raveender
authors: R
authors: Vannela
mesh_terms: D001419:Bacteria / Q000378:metabolism; D018533:Biomass; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D050356:Lipid Metabolism; D058433:Photobioreactors / Q000382:microbiology*; D046939:Synechocystis / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature
chemical_list: D004952:Esters; D005227:Fatty Acids