title: Genetic deletion of the P2Y2 receptor offers significant resistance to development of lithium-induced polyuria accompanied by alterations in PGE2 signaling.
abstract: Lithium (Li)-induced polyuria is due to resistance of the medullary collecting duct (mCD) to the action of arginine vasopressin (AVP), apparently mediated by increased production of PGE(2). We previously reported that the P2Y(2) receptor (P2Y(2)-R) antagonizes the action of AVP on the mCD and may play a role in Li-induced polyuria by enhancing the production of PGE(2) in mCD. Hence, we hypothesized that genetic deletion of P2Y(2)-R should ameliorate Li-induced polyuria. Wild-type (WT) or P2Y(2)-R knockout (KO) mice were fed normal or Li-added diets for 14 days and euthanized. Li-induced polyuria, and decreases in urine osmolality and AQP2 protein abundance in the renal medulla, were significantly less compared with WT mice despite the lack of differences in Li intake or terminal serum or inner medullary tissue Li levels. Li-induced increased urinary excretion of PGE(2) was not affected in KO mice. However, prostanoid EP(3) receptor (EP3-R) protein abundance in the renal medulla of KO mice was markedly lower vs. WT mice, irrespective of the dietary regimen. The protein abundances of other EP-Rs were not altered across the groups irrespective of the dietary regimen. Ex vivo stimulation of mCD with PGE(2) generated significantly more cAMP in Li-fed KO mice (130%) vs. Li-fed WT mice (100%). Taken together, these data suggest 1) genetic deletion of P2Y(2)-R offers significant resistance to the development of Li-induced polyuria; and 2) this resistance is apparently due to altered PGE(2) signaling mediated by a marked decrease in EP3-R protein abundance in the medulla, thus attenuating the EP3-mediated decrease in cAMP levels in mCD.
authors: Nephrology Research (151M VA SLC Health Care System, 500 Foothill Dr., Salt Lake City, UT 84148, USA.
authors: Yue
authors: Y
authors: Zhang
authors: Ioana L
authors: IL
authors: Pop
authors: Noel G
authors: NG
authors: Carlson
authors: Bellamkonda K
authors: BK
authors: Kishore
mesh_terms: D000818:Animals; D051399:Aquaporin 2 / Q000378:metabolism; D001127:Arginine Vasopressin / Q000009:adverse effects; D000242:Cyclic AMP / Q000652:urine; D018500:Diabetes Insipidus, Nephrogenic / Q000139:chemically induced*; D015232:Dinoprostone / Q000652:urine; D005260:Female; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D018021:Lithium Chloride / Q000009:adverse effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011141:Polyuria / Q000139:chemically induced; D058306:Receptors, Prostaglandin E, EP1 Subtype / Q000378:metabolism; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000378:metabolism; D058473:Receptors, Purinergic P2Y2
chemical_list: D051399:Aquaporin 2; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058473:Receptors, Purinergic P2Y2; D001127:Arginine Vasopressin; D000242:Cyclic AMP; D018021:Lithium Chloride; D015232:Dinoprostone