title: Prevalence and spectrum of microsatellite alterations in nonmuscle invasive bladder cancers.
abstract: We aimed to identify interesting deleted chromosomal regions for bladder cancer diagnosis and carcinogenesis, and to evaluate the association between loss of heterozygosity (LOH) and clinico-pathological parameters. Microsatellite analysis was performed on urine sediment and tumor tissue from 43 consecutive patients with superficial transitional cell carcinoma (TCC) and from 42 consecutive controls. Informative cases were scored as LOH or allelic loss (AL) according to the decrease of the allelic-imbalance ratio. The prevalence of LOH and AL was 39.5% and 86%, respectively. Chromosome 9 was the most frequently altered, especially at 9p (35%). The total number of microsatellite alterations per analysis was correlated with age, grade, stade and EAU classification. The locus 17p13.1 was strongly associated with high-stage (p=0.01) and high-grade tumors (p=0.02). Specificity and sensitivity of LOH was 100% and 39.3% for diagnosis of malignant urinary disease. Specificity and sensitivity of AL was 73.8% and 88%, respectively. Allelic losses are a frequent and early event in bladder cancer, especially at 9p. Thanks to its high specificity, LOH may serve as a complementary tool for non invasive diagnosis of bladder cancer. Further study is warranted to evaluate the prognostic value of LOH on recurrence, progression and muscle invasion.
authors: Department of Urology and Paris 7 University, APHP, Saint-Louis Hospital Paris, France ;  Department of Biochemistry and Paris 7 University, APHP, Saint-Louis Hospital Paris, France ;  Department of Pathology and Paris 7 University, APHP, Saint-Louis Hospital Paris, France ;  INSERM U944 UMR7212, Paris 7 University Paris, France ; and  INSERM U728 - Institut Universitaire d'Hematologe, Paris 7 University Paris, France.
authors: Guillaume
authors: G
authors: Legrand
authors: Hany
authors: H
authors: Soliman
authors: Francis
authors: F
authors: Dubosq
authors: Jérôme
authors: J
authors: Vérine
authors: François
authors: F
authors: Desgrandchamps
authors: Hugues
authors: H
authors: de Thé
authors: Pierre
authors: P
authors: Mongiat-Artus
authors: Guillaume
authors: G
authors: Ploussard