title: Pros and cons of fish skin cells in culture: long-term full skin and short-term scale cell culture from rainbow trout, Oncorhynchus mykiss.
abstract: Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We further demonstrated that epithelial cells derived from the scales survived in the artificial micro-environment of surrounding fibroblast-like cells. Also, antibody staining indicated that both cell types proliferated and started to build connections with the other cell type. It seems that it is possible to generate an 'artificial skin' with two different cell types. This could lead to the development of a three-dimensional test system, which might be a better in vitro representative of fish skin in vivo than individual skin cell lines.
authors: LG Aquatic Cell Technology, Fraunhofer Institute for Marine Biotechnology, Paul-Ehrlich-Str.1-3, 23562 Lübeck, Germany. sebastian.rakers@emb.fraunhofer.de
authors: Sebastian
authors: S
authors: Rakers
authors: Matthias
authors: M
authors: Klinger
authors: Charli
authors: C
authors: Kruse
authors: Marina
authors: M
authors: Gebert
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation; D003584:Cytological Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D017686:Oncorhynchus mykiss; D012867:Skin / Q000166:cytology*