title: Patterned domains of supported phospholipid bilayer using microcontact printing of Pll-g-PEG molecules.
abstract: In this work, we propose a reliable microcontact printing (μCP) process for generating Patterned Supported Phospholipids Bilayer (P-SPB) confined by Poly-L-(lysine)-grafted-polyethylene(glycol) (Pll-g-PEG) molecular barriers. The efficiency of Pll-g-PEG for inhibiting the fusion process of incubated liposome was first analyzed by Quartz Micro Balance (QCM) measurements. The quality and stability of Pll-g-PEG patterns were then both verified by fluorescence microscopy and Atomic Force Microscopy (AFM) in liquid media. The micro domains of P-SPB produced were stable in liquid environment during several weeks and also during AFM imaging. This exceptional stability is a clear improvement compared to previous studies involving proteins as confinement barriers.
authors: CNRS, LAAS, Toulouse, France. jchalmea@laas.fr
authors: Jerome
authors: J
authors: Chalmeau
authors: Christian
authors: C
authors: le Grimellec
authors: John
authors: J
authors: Sternick
authors: Christophe
authors: C
authors: Vieu
mesh_terms: D008051:Lipid Bilayers; D010743:Phospholipids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids; D011092:Polyethylene Glycols