title: A viscosity-dependent affinity sensor for continuous monitoring of glucose in biological fluids.
abstract: We present a viscometric affinity biosensor for continuous monitoring of glucose in biological fluids such as blood and plasma. The sensing principle of this chemico-mechanical sensor is based upon the viscosity variation of a sensitive fluid with glucose concentration. Basically, this device includes both an actuating and a sensing piezoelectric diaphragms as well as a flow-resistive microchannel. In order to confine the sensitive fluid and allow glucose diffusion into the sensor, a free-standing alumina nanoporous membrane is also used as size-selective interface. Measurements carried out at nominal temperatures of 25 and 37 °C reveal that this sensor topology exhibits a high resolution in the current range of physiological blood glucose concentrations, i.e. 2-20 mM. In addition, complete reversibility was also demonstrated for at least 3 days. Finally, measurements performed in human blood serum confirm that this sensor fulfils all basic requirements for a use in continuous glucose monitoring of biological fluids.
authors: Laboratory of Microengineering for Manufacturing, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. christophe.boss@epfl.ch
authors: Christophe
authors: C
authors: Boss
authors: Eric
authors: E
authors: Meurville
authors: Jean-Michel
authors: JM
authors: Sallese
authors: Peter
authors: P
authors: Ryser
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D001809:Blood Viscosity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D001786:Blood Glucose