title: In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology.
abstract: OBJECTIVE
  The aim of this work was to determine the relevance of Choukroun's platelet-rich fibrin (PRF) in dental implantology by determining the in vitro effects of soluble factors released by PRF clot. We used 3 different cell lines implicated in dental implantology: osteoblast, keratinocyte, and fibroblast.
  
  
  METHODS
  Cellular viability, cell proliferation, and gene expression were analyzed using PRF conditioned medium. Three different cells lines were used: SaOS2 (osteoblast), MRC5 (fibroblast), and KB (epithelial cell).
  
  
  RESULTS
  The sulforhodamine B assay showed a significant increase in cell number for the undiluted and 1:3 diluted conditioned medium after 24 and 48 hours. There was no effect for the 1:9 dilution. Cell cycle analysis by flow cytometry confirmed the viability test results. After 48 hours, PRF conditioned medium induced gene expression in osteoblasts. Expression of osteopontin and osteocalcin, late osteogenic markers, was observed using reverse transcriptase-polymerase chain reaction (RT-PCR).
  
  
  CONCLUSIONS
  This study establishes a model to evaluate, in vitro, the effects of soluble growth factors released by PRF clot. Our work confirmed PRF is useful in stimulating tissue healing and bone regeneration. This work should recommend Choukroun's PRF in numerous implantology clinical applications.
authors: Equipe de Biomatériaux en Site Osseux, UMR CNRS 6226, Sciences Chimiques de Rennes, Faculté d'Odontologie, Université de Rennes 1, Université Européenne de Bretagne, Rennes Cedex, France. fabrice.clipet@univ-rennes1.fr
authors: Fabrice
authors: F
authors: Clipet
authors: Sylvie
authors: S
authors: Tricot
authors: Nora
authors: N
authors: Alno
authors: Murielle
authors: M
authors: Massot
authors: Hélène
authors: H
authors: Solhi
authors: Guy
authors: G
authors: Cathelineau
authors: Fabienne
authors: F
authors: Perez
authors: Gilbert
authors: G
authors: De Mello
authors: Pascal
authors: P
authors: Pellen-Mussi
mesh_terms: D000704:Analysis of Variance; D001792:Blood Platelets; D001861:Bone Regeneration / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D003758:Dental Implantation, Endosseous; D023381:Endpoint Determination; D005337:Fibrin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007624:KB Cells; D015603:Keratinocytes / Q000187:drug effects*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D053495:Osteopontin / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; D006133:Growth Substances; D015675:Osteocalcin; D053495:Osteopontin; D005337:Fibrin