title: A computational statistics approach for estimating the spatial range of morphogen gradients.
abstract: A crucial issue in studies of morphogen gradients relates to their range: the distance over which they can act as direct regulators of cell signaling, gene expression and cell differentiation. To address this, we present a straightforward statistical framework that can be used in multiple developmental systems. We illustrate the developed approach by providing a point estimate and confidence interval for the spatial range of the graded distribution of nuclear Dorsal, a transcription factor that controls the dorsoventral pattern of the Drosophila embryo.
authors: Department of Chemical and Biological Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
authors: Jitendra S
authors: JS
authors: Kanodia
authors: Yoosik
authors: Y
authors: Kim
authors: Raju
authors: R
authors: Tomer
authors: Zia
authors: Z
authors: Khan
authors: Kwanghun
authors: K
authors: Chung
authors: John D
authors: JD
authors: Storey
authors: Hang
authors: H
authors: Lu
authors: Philipp J
authors: PJ
authors: Keller
authors: Stanislav Y
authors: SY
authors: Shvartsman
mesh_terms: D000818:Animals; D056808:Biostatistics / Q000379:methods*; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D019295:Computational Biology / Q000379:methods / Q000706:statistics & numerical data; D003198:Computer Simulation; D004330:Drosophila / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental / Q000502:physiology; D021621:Imaging, Three-Dimensional; D017404:In Situ Hybridization, Fluorescence; D009024:Morphogenesis / Q000235:genetics* / Q000502:physiology; D009994:Osmolar Concentration; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D029721:Drosophila Proteins