title: PTEN inhibits proliferation and functions of hypertrophic scar fibroblasts.
abstract: Hypertrophic scar (HS) remains a major problem in plastic surgery. In order to explore the regulative effect of phosphatase and tensin homolog (PTEN) on HS, PTEN and AKT expression was detected by reverse transcription PCR, immunohistochemistry and western blot. Adenovirus-mediated PTEN overexpression in cultured hypertrophic scar fibroblasts (HSFBs) and normal skin fibroblasts was also introduced to evaluate its biological function. Our results showed that PTEN expression was significantly decreased in HS whereas p-Akt level was significantly higher in HS compared with normal skin (P < 0.01). Furthermore, we found that adenovirus-mediated PTEN overexpression led to decreased AKT activation, and significantly reduced cell proliferation and collagen synthesis of HSFBs, while increased the apoptosis. Taken together, these data suggest that PTEN inhibits proliferation and function of HSFBs through AKT pathway. Our results reveal a novel biological role for PTEN/AKT pathway in HS and suggest PTEN as a potential therapeutic target for HS treatment.
authors: Department of Plastic and Reconstructive Surgery, Southwest Hospital, Third Military Medical University, Shapingba District, Chongqing, People's Republic of China.
authors: Liang
authors: L
authors: Guo
authors: Liang
authors: L
authors: Chen
authors: Sheng
authors: S
authors: Bi
authors: Linlin
authors: L
authors: Chai
authors: Zengxiang
authors: Z
authors: Wang
authors: Chuan
authors: C
authors: Cao
authors: Ling
authors: L
authors: Tao
authors: Shirong
authors: S
authors: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000201:enzymology / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000201:enzymology* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D055815:Young Adult
chemical_list: D011994:Recombinant Proteins; D003094:Collagen; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human