title: [Effect of chai qin cheng qi decoction on serum CCK-8 and calcium overload of pancreatic acinar in mice with acute pancreatitis].
abstract: OBJECTIVE
  To investigate the effect of Chai Qin Cheng Qi decoction (CQCQD) on serum cholecystokinin-8 (CCK-8) and calcium overload of pancreatic acinar cells in acute pancreatitis (AP) mice.
  
  
  METHODS
  Twenty four mice were randomly divided into control group, AP group, CQCQD group and siRNA group, each comprising 6 mice. AP mouse model was induced by intraperitoneal injection of 8% L-arginine in a dose of 4 g/kg. The AP mice in the CQCQD group were fed with 0.4 mL/100 g of Chai Qin Cheng Qi solution once every two hours. The AP mice in the siRNA group were injected intraperitoneally with CCK-siRNA in a dose of 0.88 mg/kg. The changes of serum CCK-8 and calcium concentrations in the pancreatic acinar cells and pancreatic pathology were observed 6 hours after the interventions.
  
  
  RESULTS
  The serum CCK-8 [(3764.3 +/- 369.2) ng/mL], calcium fluorescence intensity (34.8 +/- 27.1) of pancreatic acinar cells and pancreas pathology scores (6.2 +/- 1.1) of the AP mice were significantly greater (P < 0.05) than those in the control group [(1253.5 +/- 39.5) ng/mL, 5.2 +/- 2.3, 2.8 +/- 0.4], CQCQD group [(1230.5 +/- 46.1) ng/mL, 9.6 +/- 1.6, 3.8 +/- 0.8, 4.1 +/- 0.5] and siRNA group[(1702.3 +/- 598.3) ng/mL, 7.6 +/- 2.0]. Serum CCK-8 was positively correlated with intracellular calcium concentrations (r = 0.793, P = 0.021) in pancreatic acinar cells and pancreas pathology scores (r = 0.847, P = 0.000).
  
  
  CONCLUSIONS
  Acute pancreatitis in mice induced by L-arginine is associated with calcium overload in pancreatic acinar cells induced by increased serum CCK-8. CQCQD can reduce serum levels of CCK-8, alleviate calcium overload in pancreatic acinar cells, and reduce pancreas pathological changes in AP mice.
authors: Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Jia
authors: J
authors: Guo
authors: Hai-Yan
authors: HY
authors: Zhang
authors: Tao
authors: T
authors: Jin
authors: Long
authors: L
authors: Zhao
authors: Li
authors: L
authors: Wen
authors: Lei
authors: L
authors: Huang
authors: Ping
authors: P
authors: Xue
authors: Zheng-Yu
authors: ZY
authors: Cheng
authors: Xiao-Nan
authors: XN
authors: Yang
authors: Qing
authors: Q
authors: Xia
mesh_terms: D061354:Acinar Cells / Q000378:metabolism; D000208:Acute Disease; D000818:Animals; D002118:Calcium / Q000097:blood* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D008297:Male; D051379:Mice; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D010195:Pancreatitis / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D011897:Random Allocation; D012844:Sincalide / Q000097:blood* / Q000378:metabolism
chemical_list: C528108:Chai Qin Cheng Qi decoction; D004365:Drugs, Chinese Herbal; D012844:Sincalide; D002118:Calcium