title: Identification of genes expressed in juvenile Haliotis rufescens in response to different copper concentrations in the north of Chile under controlled conditions.
abstract: This study reports molecular markers potentially associated with resistance or sensitivity to the impact of copper in juvenile red abalone, Haliotis rufescens, in the north of Chile under experimental conditions. Genomic analysis was made applying subtractive hybridization libraries (SSH) to identify genes up-and down regulated during cooper exposure in abalone over periods of 12 and 168 h exposed to 2.5 and 10 μg/L of Cu(+2). Results obtained from the SSH library revealed 368 different sequences regulated by copper, that correspond to eight major physiological functions. The validation of these sequences obtained by SSH as well as their expression kinetics were made by PCR in real time on 14 potential genes regulated by metal stress. This study provides information for the characterization of potential genomic markers that may be used in future environmental monitoring and to investigate new mechanisms of stress to copper in this commercially important marine species.
authors: Laboratorio de Ecología Microbiana, Universidad de Antofagasta, 170 Antofagasta, Chile. fsaciares@uantof.cl
authors: Fernando
authors: F
authors: Silva-Aciares
authors: Manuel
authors: M
authors: Zapata
authors: Jennifer
authors: J
authors: Tournois
authors: Dario
authors: D
authors: Moraga
authors: Carlos
authors: C
authors: Riquelme
mesh_terms: D000818:Animals; D002677:Chile; D003300:Copper / Q000378:metabolism* / Q000633:toxicity; D004784:Environmental Monitoring; D020224:Expressed Sequence Tags; D049851:Gastropoda / Q000187:drug effects* / Q000235:genetics*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper