title: Bacterial predators possess unique membrane lipid structures.
abstract: Bdellovibrio-and-like organisms (BALO) are a phylogenetically diverse group of predatory prokaryotes that consists of the two families Bdellovibrionaceae and Bacteriovoracaceae. We investigated the phospholipid composition of the three important BALO strains Bacteriovorax stolpii (DSM 12778), Bdellovibrio bacteriovorus HD100 (DSM 50701) and Peredibacter starrii (DSM 17039). We confirmed the presence of sphingophosphonolipids in B. stolpii, while we characterized sphingophosphonolipids with a 2-amino-3-phosphonopropanate head group for the first time. In B. bacteriovorus HD100 phosphatidylthreonines were found and, thus, B.                         bacteriovorus is the second prokaryote investigated so far possessing this rare lipid class. In the third analyzed organism, P. starrii, we observed phosphatidylethanolamine structures with an additional N-glutamyl residue, which form the first reported class of amino acid-containing phosphatidylethanolamines.
authors: Department of Chemistry, Humboldt-Universität zu Berlin, Germany.
authors: Frederic D
authors: FD
authors: Müller
authors: Sebastian
authors: S
authors: Beck
authors: Eckhard
authors: E
authors: Strauch
authors: Michael W
authors: MW
authors: Linscheid
mesh_terms: D000898:Antibiosis / Q000502:physiology; D001501:Bdellovibrio / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D020564:Deltaproteobacteria / Q000737:chemistry*; D005971:Glutamates / Q000378:metabolism; D020404:Glycerophospholipids / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis; D013107:Sphingolipids / Q000032:analysis; D053719:Tandem Mass Spectrometry; D013912:Threonine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D005971:Glutamates; D020404:Glycerophospholipids; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D013107:Sphingolipids; C017629:phosphatidylthreonine; D013912:Threonine; C483858:phosphatidylethanolamine