title: Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol.
abstract: OBJECTIVE
  To determine the concentrations of angiopoietin 1 (ANGPT1) and ANGPT2 in individual human preovulatory follicles in relation to their diameter or volume to clarify the role of these molecules in folliculogenesis.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Research laboratory at Kansai Medical University, Osaka, Japan.
  
  
  METHODS
  Twenty-three women undergoing IVF.
  
  
  METHODS
  On the day of oocyte retrieval, serum samples and follicular fluid (FF) from individual follicles were collected. We analyzed 348 follicles.
  
  
  METHODS
  ANGPT1 and ANGPT2 concentrations in FF and serum and oocyte recovery rates.
  
  
  RESULTS
  On average, ANGPT1 concentrations in FF were 150 times lower than those in serum, whereas ANGPT2 concentrations in FF were 8 times higher than those in serum. The concentrations of ANGPT1 in follicles with a diameter ≤17 mm were significantly higher than those in follicles with a diameter ≥18 mm. On the other hand, the concentrations of ANGPT2 in follicles with a diameter ≤17 mm were significantly lower than those in follicles with a diameter ≥18 mm. The ANGPT2/ANGPT1 ratio increased with enlargement of follicular diameter. ANGPT1 concentrations in FF decreased with follicular volume. ANGPT2 concentrations and the ANGPT2/ANGPT1 ratio in FF rose with follicular volume. The ANGPT2/ANGPT1 ratio in FF from the oocyte recovery group was significantly higher than that from the nonrecovery group.
  
  
  CONCLUSIONS
  Our data suggested that the change in ANGPT1 and ANGPT2 levels may be associated with follicular growth and angiogenesis during the preovulatory period.
authors: Department of Obstetrics and Gynecology, Kansai Medical University, Osaka, Japan.
authors: Akemi
authors: A
authors: Nishigaki
authors: Hidetaka
authors: H
authors: Okada
authors: Tomoko
authors: T
authors: Tsuzuki
authors: Hisayuu
authors: H
authors: Cho
authors: Katsuhiko
authors: K
authors: Yasuda
authors: Hideharu
authors: H
authors: Kanzaki
mesh_terms: D000328:Adult; D042683:Angiopoietin-1 / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D048429:Cell Size; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007246:Infertility / Q000097:blood / Q000378:metabolism / Q000628:therapy*; D008297:Male; D054315:Oocyte Retrieval / Q000706:statistics & numerical data; D009994:Osmolar Concentration; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D010060:Ovulation / Q000378:metabolism; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2