title: Isolation and characterization of latent and active polyphenoloxidase in BRS Clara (CNPUV 154-147 × Centennial seedless) and BRS Morena (Marroo seedless × Centennial seedless) seedless table grapes.
abstract: The seedless grapes BRS Clara and BRS Morena, developed in Brazil, are currently growing in popularity due to their premium texture and taste. However, there are no reports on the polyphenoloxidase (PPO) from these cultivars. In this paper, active and latent PPO from BRS Clara and BRS Morena seedless grapes were extracted using the non-ionic detergents Triton-X-100 (active) and Triton-X-114 (latent), and their catecholase activities were characterized. The PPO extracted using Triton-X-110 exhibited maximum activities at pH 6.0 and at 25 °C. Above 30 °C, a gradual decline in activities was noted, with complete inactivation at 60 °C. The PPO from grapes extracted with Triton-X-114 was activated with 0.2% of the ionic detergent sodium dodecyl sulfate (SDS), and exhibited maximum activities at pH 5.5 and at 30 °C. It was stable until the temperature reached 60 °C.
authors: Laboratory of Biochemistry and Applied Microbiology, São Paulo State University, UNESP, Rua Cristovão Colombo 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, São Paulo, Brazil. ellenlagovanzela@hotmail.com
authors: Ellen Silva
authors: ES
authors: Lago-Vanzela
authors: Fabiana Carina
authors: FC
authors: Pavezzi
authors: Natália
authors: N
authors: Martin
authors: Eleni
authors: E
authors: Gomes
authors: Roberto
authors: R
authors: Da Silva
mesh_terms: D001938:Brazil; D004156:Catechol Oxidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents; D004789:Enzyme Activation; D004795:Enzyme Stability; D005638:Fruit / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D017830:Octoxynol; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D027843:Vitis / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D003902:Detergents; D007527:Isoenzymes; D010636:Phenols; D010936:Plant Extracts; D010940:Plant Proteins; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; D004156:Catechol Oxidase