title: Activation of signal transducers and activators of transcription 3 in the hippocampal CA1 region in a rat model of global cerebral ischemic preconditioning.
abstract: The signal transducers and activators of transcription 3 (STAT3) has been suggested to have neuroprotective roles. However, its role in ischemic preconditioning (PC) is still obscure. In this study, we examined the phosphorylation status of ser727-STAT3, which is necessary for activation of STAT3, and its roles in a rat global ischemia model with or without PC. PC was induced by 3 min of nonlethal ischemia 48 h before 5 min of lethal ischemia. Western blot analysis showed that phospho-ser727-STAT3 significantly increased from 8 to 48 h after nonlethal ischemia, while it increased only for 1h after lethal ischemia and returned to the baseline within 24h. In the preconditioned brains, phospho-ser727-STAT3 was induced at 1 to 4h after lethal ischemia, and decrease of its levels delayed compared to the nonconditioned brains. Immunohistochemistry revealed that phospho-ser727-STAT3 was expressed mainly in CA1 neurons after nonlethal ischemia. Additionally, STAT3 inhibitor peptide treatment prevented PC induced-neuroprotection. These results indicate that phosphorylation of ser727-STAT3 plays an important role in PC induced- neuroptotection.
authors: Department of Neurosurgery, Interdisciplinary graduate school of medicine and engineering, University of Yamanashi, Japan.
authors: Takashi
authors: T
authors: Yagi
authors: Hideyuki
authors: H
authors: Yoshioka
authors: Takuma
authors: T
authors: Wakai
authors: Tatsuya
authors: T
authors: Kato
authors: Toru
authors: T
authors: Horikoshi
authors: Hiroyuki
authors: H
authors: Kinouchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology* / Q000503:physiopathology / Q000628:therapy*; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D004195:Disease Models, Animal; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D010766:Phosphorylation / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat