title: N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO.
abstract: We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.
authors: Department of Chemistry, University of Chicago, Chicago, Illinois, USA.
authors: Guifang
authors: G
authors: Jia
authors: Ye
authors: Y
authors: Fu
authors: Xu
authors: X
authors: Zhao
authors: Qing
authors: Q
authors: Dai
authors: Guanqun
authors: G
authors: Zheng
authors: Ying
authors: Y
authors: Yang
authors: Chengqi
authors: C
authors: Yi
authors: Tomas
authors: T
authors: Lindahl
authors: Tao
authors: T
authors: Pan
authors: Yun-Gui
authors: YG
authors: Yang
authors: Chuan
authors: C
authors: He
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008745:Methylation; D009765:Obesity; D010084:Oxidation-Reduction; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011506:Proteins; D012333:RNA, Messenger; C010223:N-methyladenosine; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human; D000241:Adenosine