title: Alishewanella tabrizica sp. nov., isolated from Qurugol Lake.
abstract: A novel Gram-negative, aerobic, motile and rod-shaped bacterium was isolated from Qurugöl Lake located in a mountainous region near Tabriz city in the north-west of Iran. Growth occurred at pH 6-10 (optimum, pH 7 ± 0.5) and at 10-40 °C (optimum, 30 °C). Strain RCRI4(T) was able to grow in the absence and presence of NaCl to 3% (w/v). The major fatty acids were C(17:0), C(16:1)ω7c/C(15 )iso3-OH, C(17:1)ω8c and C(16:0). The G+C content of genomic DNA was 45.3 mol%. Based on the 16S rRNA and gyrB gene sequences, phylogenetic analyses indicated that strain RCRI4(T) associated with the genus Alishewanella, and closely related type strains include Alishewanella agri BLO6(T) (97.8%), Alishewanella aestuarii B11(T) (97.7%), Rheinheimera perlucida BA131(T) (97.5%), Alishewanella fetalis CCUG 30811(T) (97.3%) and Alishewanella jeotgali MS1(T) (97.1%). The level of DNA-DNA relatedness between strain RCRI4(T) and phylogenetically the closest related strains, A. agri BLO6(T) and R. perlucida BA131(T), was 9 and 14%, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic results, it is suggested that strain RCRI4(T) represents a novel species of the genus Alishewanella, for which the name Alishewanella tabrizica sp. nov. is proposed. The type strain is RCRI4(T) ( = LMG 26473(T) = JCM 17275(T) = KCTC 23723(T)).
authors: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.|Rice and Citrus Research Institute (RCRI), Sari, Iran.
authors: Vahideh
authors: V
authors: Tarhriz
authors: Faculty of Agriculture, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran.|Rice and Citrus Research Institute (RCRI), Sari, Iran.
authors: Ghorbanali
authors: G
authors: Nematzadeh
authors: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Sepideh
authors: S
authors: Zununi Vahed
authors: Agricultural Biotechnology Research Institute (ABRII) for Northwest and West of Iran, Tabriz, Iran.
authors: Mohammad Amin
authors: MA
authors: Hejazi
authors: Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences & Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.|Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Mohammad Saeid
authors: MS
authors: Hejazi
mesh_terms: D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007492:Iran; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S