title: [Relationship between follicle stimulating hormone and AZF microdeletion on Y chromosome in patients with azoospermia or severe oligozoospermia].
abstract: OBJECTIVE
  To investigate the relationship between follicle stimulating hormone (FSH) and AZF microdeletion on Y chromosome.
  
  
  METHODS
  Fifteen loci of 4 regions of the AZF gene were investigated by multiplex PCR in 100 patients with azoospermia or severe oligozoospermia. The reproductive hormone FSH was detected by access 2 immunoassay system from BECKMAN COULTER. Epidata was set up and analyzed for means. F test of anova was performed.
  
  
  RESULTS
  The rate of microdeletion was 13% (13 out of 100 patients). The deletion was on AZFa in 1 patient, AZFb+c+d in 4 patients, AZFc+d in 7 patients, AZFd in 1 patient, respectively. The level of FSH (40.8±11.3 U/L) in the AZFb+c+d deletion group was significantly higher than that in the group without Y chromosome deletion (16.7±14.3 U/L) and the other types of deletion (11.8±6.7 U/L) (P<0.01).
  
  
  CONCLUSIONS
  The common microdeletion regions were AZFc and AZFd on Y chromosome in azoospermia or severe oligozoospermia. The microdeletion of AZFb+c+d was one of the important causes of the high level of FSH.
authors: Department of Genetics, Qingdao Research Institute for Population and Family Planning, Qingdao, Shandong, People's Republic of China.
authors: Xue-qian
authors: XQ
authors: Wang
authors: Hong-yan
authors: HY
authors: Zhang
authors: Qi-wei
authors: QW
authors: Qi
authors: Jing
authors: J
authors: Zhao
authors: Li
authors: L
authors: Xu
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000097:blood / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000097:blood*; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D005640:Follicle Stimulating Hormone