title: A primary microcephaly protein complex forms a ring around parental centrioles.
abstract: Autosomal recessive primary microcephaly (MCPH) is characterized by a substantial reduction in prenatal human brain growth without alteration of the cerebral architecture and is caused by biallelic mutations in genes coding for a subset of centrosomal proteins. Although at least three of these proteins have been implicated in centrosome duplication, the nature of the centrosome dysfunction that underlies the neurodevelopmental defect in MCPH is unclear. Here we report a homozygous MCPH-causing mutation in human CEP63. CEP63 forms a complex with another MCPH protein, CEP152, a conserved centrosome duplication factor. Together, these two proteins are essential for maintaining normal centrosome numbers in cells. Using super-resolution microscopy, we found that CEP63 and CEP152 co-localize in a discrete ring around the proximal end of the parental centriole, a pattern specifically disrupted in CEP63-deficient cells derived from patients with MCPH. This work suggests that the CEP152-CEP63 ring-like structure ensures normal neurodevelopment and that its impairment particularly affects human cerebral cortex growth.
authors: Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge, UK.
authors: Joo-Hee
authors: JH
authors: Sir
authors: Alexis R
authors: AR
authors: Barr
authors: Adeline K
authors: AK
authors: Nicholas
authors: Ofelia P
authors: OP
authors: Carvalho
authors: Maryam
authors: M
authors: Khurshid
authors: Alex
authors: A
authors: Sossick
authors: Stefanie
authors: S
authors: Reichelt
authors: Clive
authors: C
authors: D'Santos
authors: C Geoffrey
authors: CG
authors: Woods
authors: Fanni
authors: F
authors: Gergely
mesh_terms: D002502:Centrioles / Q000378:metabolism*; D002893:Chromosomes, Human, Pair 3; D005260:Female; D006801:Humans; D008297:Male; D008831:Microcephaly / Q000235:genetics / Q000378:metabolism*; D010375:Pedigree