title: Lead acetate and arsenic trioxide induce instability of microsatellites at three different fragile sites (6q21, 9q32-9q33 and 15p14) within the genome of the rat.
abstract: Human exposure to metals is of increasing concern due to the well-documented toxic and carcinogenic effects of metals and metal compounds, and the rising environmental levels due to industrial processes and pollution. It has been reported that metals can be genotoxic by several modes of action including generation of reactive oxygen species and inhibition of DNA repair. The aim of this study was to evaluate microsatellite instability (MSI) in three microsatellite loci (D6mit3, D9mit2 and D15Mgh1) located within three common fragile sites in the genome of the laboratory rat (6q21, 9q32-9q33 and 15p14) exposed to acute and chronic doses of a metal salt (lead acetate trihydrate) and a metalloid oxide (arsenic trioxide). In the acute and sub-chronic studies with the two compounds, MSI was observed in the three loci as deletions or additions of microsatellite repeats. The percentages of MSI were 36.4% and 42.1% for lead acetate and arsenic trioxide, respectively. Results of the present work indicate that the microsatellites located within fragile sites provide a convenient assay system to detect changes in DNA sequences resulting from exposure to genotoxic agents.
authors: Zoology Department, Faculty of Science, Cairo University, Egypt.
authors: Akmal A
authors: AA
authors: El-Ghor
authors: Magda M
authors: MM
authors: Noshy
authors: Jehane I
authors: JI
authors: Eid
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D043283:Chromosome Fragile Sites / Q000187:drug effects*; D008297:Male; D053842:Microsatellite Instability / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D010087:Oxides / Q000633:toxicity*; D051381:Rats
chemical_list: D001152:Arsenicals; D009153:Mutagens; D009942:Organometallic Compounds; D010087:Oxides; C008261:lead acetate; D000077237:Arsenic Trioxide