title: RNA-Seq analysis of a soybean near-isogenic line carrying bacterial leaf pustule-resistant and -susceptible alleles.
abstract: Bacterial leaf pustule (BLP) disease is caused by Xanthomonas axonopodis pv. glycines (Xag). To investigate the plant basal defence mechanisms induced in response to Xag, differential gene expression in near-isogenic lines (NILs) of BLP-susceptible and BLP-resistant soybean was analysed by RNA-Seq. Of a total of 46 367 genes that were mapped to soybean genome reference sequences, 1978 and 783 genes were found to be up- and down-regulated, respectively, in the BLP-resistant NIL relative to the BLP-susceptible NIL at 0, 6, and 12h after inoculation (hai). Clustering analysis revealed that these genes could be grouped into 10 clusters with different expression patterns. Functional annotation based on gene ontology (GO) categories was carried out. Among the putative soybean defence response genes identified (GO:0006952), 134 exhibited significant differences in expression between the BLP-resistant and -susceptible NILs. In particular, pathogen-associated molecular pattern (PAMP) and damage-associated molecular pattern (DAMP) receptors and the genes induced by these receptors were highly expressed at 0 hai in the BLP-resistant NIL. Additionally, pathogenesis-related (PR)-1 and -14 were highly expressed at 0 hai, and PR-3, -6, and -12 were highly expressed at 12 hai. There were also significant differences in the expression of the core JA-signalling components MYC2 and JASMONATE ZIM-motif. These results indicate that powerful basal defence mechanisms involved in the recognition of PAMPs or DAMPs and a high level of accumulation of defence-related gene products may contribute to BLP resistance in soybean.
authors: Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San, Sillim-dong, Gwanak-gu, Republic of Korea.
authors: Kil Hyun
authors: KH
authors: Kim
authors: Yang Jae
authors: YJ
authors: Kang
authors: Dong Hyun
authors: DH
authors: Kim
authors: Min Young
authors: MY
authors: Yoon
authors: Jung-Kyung
authors: JK
authors: Moon
authors: Moon Young
authors: MY
authors: Kim
authors: Kyujung
authors: K
authors: Van
authors: Suk-Ha
authors: SH
authors: Lee
mesh_terms: D000483:Alleles; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D030821:Plants, Genetically Modified; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA; D013025:Glycine max / Q000235:genetics* / Q000276:immunology