title: The Neurospora crassa TOB complex: analysis of the topology and function of Tob38 and Tob37.
abstract: The TOB or SAM complex is responsible for assembling several proteins into the mitochondrial outer membrane, including all β-barrel proteins. We have identified several forms of the complex in Neurospora crassa. One form contains Tob55, Tob38, and Tob37; another contains these three subunits plus the Mdm10 protein; while additional complexes contain only Tob55. As previously shown for Tob55, both Tob37 and Tob38 are essential for viability of the organism. Mitochondria deficient in Tob37 or Tob38 have reduced ability to assemble β-barrel proteins. The function of two hydrophobic domains in the C-terminal region of the Tob37 protein was investigated. Mutant Tob37 proteins lacking either or both of these regions are able to restore viability to cells lacking the protein. One of the domains was found to anchor the protein to the outer mitochondrial membrane but was not necessary for targeting or association of the protein with mitochondria. Examination of the import properties of mitochondria containing Tob37 with deletions of the hydrophobic domains reveals that the topology of Tob37 may be important for interactions between specific classes of β-barrel precursors and the TOB complex.
authors: Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
authors: Sebastian W K
authors: SW
authors: Lackey
authors: Jeremy G
authors: JG
authors: Wideman
authors: Erin K
authors: EK
authors: Kennedy
authors: Nancy E
authors: NE
authors: Go
authors: Frank E
authors: FE
authors: Nargang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000166:cytology / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D005656:Fungal Proteins