title: Protective effect of boric acid against carbon tetrachloride-induced hepatotoxicity in mice.
abstract: The protective effect of boric acid against liver damage was evaluated by its attenuation of carbon tetrachloride (CCl(4))-induced hepatotoxicity in mice. Male albino mice were treated intraperitoneally (i.p.) with boric acid (50, 100, and 200 mg/kg) or silymarin daily for 7 days and received 0.2% CCl(4) in olive oil (10 mL/kg, i.p.) on day 7. Results showed that administration of boric acid significantly reduced the elevation in serum levels of aspartate aminotransferase, alkaline phosphatase, alanine aminotransferase, and the level of malondialdehyde in the liver that were induced by CCl(4) in mice. Boric acid treatment significantly increased glutathione content, as well as the activities of superoxide dismutase and catalase in the liver. Boric acid treatment improved the catalytic activity of cytochrome P450 2E1 and maintained activation of nuclear factor kappa light-chain enhancer of activated B cell gene expression, with no effect on inducible nitric oxide synthase gene expression in the livers of mice. Histopathologically, clear decreases in the severity of CCl(4)-induced lesions were observed, particularly at high boric acid concentrations. Results suggest that boric acid exhibits potent hepatoprotective effects on CCl(4)-induced liver damage in mice, likely the result of both the increase in antioxidant-defense system activity and the inhibition of lipid peroxidation.
authors: Department of Pharmacology and Toxicology, Afyon Kocatepe University, Faculty of Veterinary Medicine, Afyonkarahisar, Turkey. since@aku.edu.tr
authors: Sinan
authors: S
authors: Ince
authors: Hikmet
authors: H
authors: Keles
authors: Metin
authors: M
authors: Erdogan
authors: Omer
authors: O
authors: Hazman
authors: Ismail
authors: I
authors: Kucukkurt
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001888:Boric Acids / Q000008:administration & dosage / Q000494:pharmacology*; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012838:Silymarin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001888:Boric Acids; D017931:DNA Primers; D012838:Silymarin; D008315:Malondialdehyde; D002374:Catalase; D019392:Cytochrome P-450 CYP2E1; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005978:Glutathione; C032688:boric acid