title: Nutrient improvement using statistical optimization for growth of Schizophyllum commune, and its antifungal activity against wood degrading fungi of rubberwood.
abstract: Two statistical tools, Plackett-Burman design (PBD) and Box-Behnken design (BBD) were used to optimize the mycelia growth of Schizophyllum commune with different nutrient components. Results showed that 32.92 g/L of biomass were produced using a medium consisting of 18.74 g/L yeast extract, 38.65 g/L glucose, and 0.59 g/L MgSO(4).7H(2)O. The experimental data fitted well with the model predicted values within 0.09 to 0.77% error. The biomass was also tested for antifungal activity against wood degrading fungi of rubberwood. Results showed that the minimum inhibitory concentration (MIC) values for antifungal activity range from 0.16 to 5.00 μg/μL. The GC-MS analysis indicated that this fungus produced several compounds, such as glycerin, 2(3H)-furanone, 5-heptyldihydro-, 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, and triacetin.
authors: School of Chemical Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Seberang Perai South, Penang, Malaysia.
authors: Yi Peng
authors: YP
authors: Teoh
authors: Mashitah Mat
authors: MM
authors: Don
authors: Salmiah
authors: S
authors: Ujang
mesh_terms: D018533:Biomass; D003470:Culture Media / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D015233:Models, Statistical; D015203:Reproducibility of Results; D012567:Schizophyllum / Q000254:growth & development* / Q000378:metabolism; D014934:Wood / Q000382:microbiology*
chemical_list: D003470:Culture Media; D005659:Fungicides, Industrial