title: Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase.
abstract: Arginine analogs were incorporated site-specifically into proteins using an in vitro translation system. In this system, mRNAs containing a CGGG codon were translated by an aminoacyl-tRNA(CCCG), which was charged with arginine analogs using yeast arginyl-tRNA synthetase. N(G)-monomethyl-L-arginine, L-citrulline and L-homoarginine were incorporated successfully into proteins using this method. The influence of arginine monomethylation in histone H3 on the acetylation of lysine residues by histone acetyltransferase hGCN5 was investigated, and the results demonstrated that K9 acetylation was suppressed by the methylation of R8 and R17 but not by R26 methylation. K18 acetylation was not affected by the methylation of R8, R17 and R26. This site-specific modification strategy provides a way to explore the roles of post-translational modifications in the absence of heterogeneity due to other modifications.
authors: Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.
authors: Akiya
authors: A
authors: Akahoshi
authors: Yoshitaka
authors: Y
authors: Suzue
authors: Mizuki
authors: M
authors: Kitamatsu
authors: Masahiko
authors: M
authors: Sisido
authors: Takashi
authors: T
authors: Ohtsuki
mesh_terms: D000595:Amino Acid Sequence; D049148:Aminoacylation; D001120:Arginine / Q000031:analogs & derivatives* / Q000235:genetics; D001126:Arginine-tRNA Ligase / Q000737:chemistry*; D002384:Catalysis; D002956:Citrulline / Q000737:chemistry / Q000235:genetics; D006709:Homoarginine / Q000737:chemistry / Q000235:genetics; D008745:Methylation; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D019323:omega-N-Methylarginine / Q000737:chemistry / Q000235:genetics
chemical_list: D006709:Homoarginine; D019323:omega-N-Methylarginine; D002956:Citrulline; D001120:Arginine; D001126:Arginine-tRNA Ligase