title: Changes of sodium iodide symporter regulated by IGF-I and TGF-β1 in mammary gland cells from lactating mice at different iodine levels.
abstract: The sodium/iodide symporter (SLC5A5, also known as NIS) is a transmembrane glycoprotein. Physiologically, iodide transportation in the mammary gland occurs during late pregnancy and lactation. To identify factors that may regulate this process at different iodine levels, we have studied the expression of NIS gene and protein in cultured mammary gland explants from lactating mice by real-time quantitative PCR and In-Cell Western methods. Mammary gland cells were grown in media with different levels of iodine for 24 h. The iodine treatment groups consist of low iodine group I (LI-I, 0 μg/l), low iodine group II (LI-II, 5 μg/l), control group (C, 50 μg/l), high iodine group I (HI-I, 3,000 μg/l), and high iodine group II (HI-II, 10,000 μg/l). The cells were then incubated with or without insulin-like growth factor I (IGF-I) or transforming growth factor β1 (TGF-β1) for another 24 h. We found that iodine inhibited NIS mRNA and protein expression in a dose-dependent manner. IGF-I and TGF-β1 further decreased NIS mRNA and protein expression that iodine inhibited at different iodine levels. In summary, we have shown that iodine downregulated NIS expression in cultured mammary gland explants from the lactating mouse. IGF-I and TGF-β1 inhibited NIS mRNA and protein expression in the mammary gland under different iodine levels.
authors: Harbin Center for Disease Control and Prevention, 30 Weixing Road, Daowai District, 150056, Harbin, China.
authors: Xue
authors: X
authors: Yu
authors: Hongmei
authors: H
authors: Shen
authors: Lixiang
authors: L
authors: Liu
authors: Lin
authors: L
authors: Lin
authors: Meili
authors: M
authors: Gao
authors: Shinan
authors: S
authors: Wang
mesh_terms: D000818:Animals; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007455:Iodine / Q000378:metabolism*; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D000099209:Sodium-Iodide Symporters
chemical_list: D007334:Insulin-Like Growth Factor I; D007455:Iodine; D012333:RNA, Messenger; D027981:Symporters; D053773:Transforming Growth Factor beta1; D000099209:Sodium-Iodide Symporters