title: Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice.
abstract: The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP(SAT), demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues.
authors: Morphogenesis and Intracellular Signaling, Institut Curie-UMR144 CNRS, Paris, France.
authors: Silvia
authors: S
authors: Fre
authors: Edouard
authors: E
authors: Hannezo
authors: Sanja
authors: S
authors: Sale
authors: Mathilde
authors: M
authors: Huyghe
authors: Daniel
authors: D
authors: Lafkas
authors: Holger
authors: H
authors: Kissel
authors: Angeliki
authors: A
authors: Louvi
authors: Jeffrey
authors: J
authors: Greve
authors: Daniel
authors: D
authors: Louvard
authors: Spyros
authors: S
authors: Artavanis-Tsakonas
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002999:Clone Cells; D020895:Enterocytes / Q000166:cytology / Q000378:metabolism; D055879:Gene Knock-In Techniques; D018390:Gene Targeting; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D007422:Intestines / Q000166:cytology*; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008871:Microvilli / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000072056:Transcription Factor HES-1; D014158:Transcription, Genetic
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D019426:Integrases; D051880:Receptors, Notch; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse; C045073:Cre recombinase