title: Implementation of flow cytometry in the diagnostic work-up of myelodysplastic syndromes in a multicenter approach: report from the Dutch Working Party on Flow Cytometry in MDS.
abstract: Flow cytometry (FC) is recognized as an important tool in the diagnosis of myelodysplastic syndromes (MDS) especially when standard criteria fail. A working group within the Dutch Society of Cytometry aimed to implement FC in the diagnostic work-up of MDS. Hereto, guidelines for data acquisition, analysis and interpretation were formulated. Based on discussions on analyses of list mode data files and fresh MDS bone marrow samples and recent literature, the guidelines were modified. Over the years (2005-2011), the concordance between the participating centers increased indicating that the proposed guidelines contributed to a more objective, standardized FC analysis, thereby ratifying the implementation of FC in MDS.
authors: Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands. tm.westers@vumc.nl
authors: Theresia M
authors: TM
authors: Westers
authors: Vincent H J
authors: VH
authors: van der Velden
authors: Canan
authors: C
authors: Alhan
authors: Roelof
authors: R
authors: Bekkema
authors: André
authors: A
authors: Bijkerk
authors: Rik A
authors: RA
authors: Brooimans
authors: Claudia
authors: C
authors: Cali
authors: Angelika M
authors: AM
authors: Dräger
authors: Valerie
authors: V
authors: de Haas
authors: Christa
authors: C
authors: Homburg
authors: Anja
authors: A
authors: de Jong
authors: P Ellen A
authors: PE
authors: Kuiper-Kramer
authors: Marije
authors: M
authors: Leenders
authors: Ingrid
authors: I
authors: Lommerse
authors: Jeroen G
authors: JG
authors: te Marvelde
authors: Joke K
authors: JK
authors: van der Molen-Sinke
authors: Bijan
authors: B
authors: Moshaver
authors: Andre B
authors: AB
authors: Mulder
authors: Frank W M B
authors: FW
authors: Preijers
authors: Roger K
authors: RK
authors: Schindhelm
authors: Alita
authors: A
authors: van der Sluijs
authors: Elisabeth R
authors: ER
authors: van Wering
authors: August H
authors: AH
authors: Westra
authors: Arjan A
authors: AA
authors: van de Loosdrecht
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002469:Cell Separation / Q000379:methods / Q000592:standards; D005260:Female; D005434:Flow Cytometry / Q000379:methods / Q000592:standards*; D019983:Guideline Adherence; D006801:Humans; D008297:Male; D009190:Myelodysplastic Syndromes / Q000175:diagnosis*; D009426:Netherlands; D017410:Practice Guidelines as Topic