title: Sphingobacterium nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco.
abstract: A novel Gram-negative strain, designated M-SX103(T), was isolated from a fresh leaf of Nicotiana tabacum Linn. collected at Yuxi City, Yunnan province, China, and subjected to a polyphasic taxonomic study. Growth occurred at 10-40 °C, at pH 6.0-9.0 and with 0-3% (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids of strain M-SX103(T) were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c). The G+C content of the genomic DNA was 40.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Sphingobacterium, sharing 95.4-89.7% sequence similarity with type strains of members of the genus Sphingobacterium; it was most closely related to Sphingobacterium composti T5-12(T). On the basis of the taxonomic evidence, a novel species, Sphingobacterium nematocida sp. nov., is proposed. The type strain is M-SX103(T) ( = JCM 17339(T) = CCTCC AB 2010390(T)).
authors: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Jie
authors: J
authors: Liu
authors: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Ling-Ling
authors: LL
authors: Yang
authors: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Chuan-Kun
authors: CK
authors: Xu
authors: Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, PR China.
authors: Jia-Qin
authors: JQ
authors: Xi
authors: Yunnan Yunye Fertilizer Co. Ltd, Kunming 650217, PR China.
authors: Fa-Xiang
authors: FX
authors: Yang
authors: Shandong TaiFengYuan Biotechnology Co. Ltd, Jinan 250014, PR China.
authors: Feng
authors: F
authors: Zhou
authors: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Yu
authors: Y
authors: Zhou
authors: Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Ming-He
authors: MH
authors: Mo
authors: Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi 830011, PR China.|Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, PR China.
authors: Wen-Jun
authors: WJ
authors: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S