title: Involvement of CLEC16A in activation of astrocytes after LPS treated.
abstract: CLEC16A, C-type lectin domain family 16, member A was recently found to be associated with inflation process in the autoimmune diseases. In this study, we elucidated the dynamic expression changes and localization of CLEC16A in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CLEC16A expression was strongly induced in active astrocytes in inflamed cerebral cortex. In vitro studies indicated that the up-regulation of CLEC16A may be involved in the subsequent astrocyte activation following LPS challenge. And Knock-down of CLEC16A in cultured primary astrocytes by siRNA showed that CLEC16A was required for the activation of astrocytes induced by LPS. Collectively, these results suggested CLEC16A may be important in host defense in astrocyte-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammtion in CNS.
authors: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001 Jiangsu, People's Republic of China.
authors: Xinmin
authors: X
authors: Wu
authors: Jing
authors: J
authors: Li
authors: Chun
authors: C
authors: Chen
authors: Yaohua
authors: Y
authors: Yan
authors: Shengyang
authors: S
authors: Jiang
authors: Xiaohong
authors: X
authors: Wu
authors: Bai
authors: B
authors: Shao
authors: Jin
authors: J
authors: Xu
authors: Lihua
authors: L
authors: Kang
authors: Yeqing
authors: Y
authors: Huang
authors: Lin
authors: L
authors: Zhu
authors: Yuhong
authors: Y
authors: Ji
authors: Yilu
authors: Y
authors: Gao
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D037181:Lectins, C-Type / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C571937:CLEC16A protein, rat; D017931:DNA Primers; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D009004:Monosaccharide Transport Proteins