title: Derivation of smooth muscle cells with neural crest origin from human induced pluripotent stem cells.
abstract: The heterogeneity of vascular smooth muscle cells (SMCs) is related to their different developmental origins such as the neural crest and mesoderm. Derivation of SMCs from different origins will provide valuable in vitro models for the investigation of vascular development and diseases. From the perspective of regenerative medicine and tissue engineering, an expandable cell source of SMCs is required for the construction of tissue-engineered blood vessels. In this study, we developed a robust protocol to derive neural crest stem cells (NCSCs) from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). NCSCs derived from ESCs and iPSCs were expandable with similar cell doubling times. NCSCs were capable of differentiating into neural and mesenchymal lineages. TGF-β1 induced the expression of SMC markers calponin-1, SM22α, and smooth muscle myosin heavy chain and resulted in the assembly of smooth muscle α-actin, calponin-1, and SM22α into stress fibers. This work provides a basis for using iPSCs to study SMC biology and deriving vascular cells for tissue engineering.
authors: Department of Bioengineering, University of California, Berkeley, USA.
authors: Aijun
authors: A
authors: Wang
authors: Zhenyu
authors: Z
authors: Tang
authors: Xian
authors: X
authors: Li
authors: Yisu
authors: Y
authors: Jiang
authors: Danielle A
authors: DA
authors: Tsou
authors: Song
authors: S
authors: Li
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D009432:Neural Crest / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*