title: Transcriptome map of mouse isochores.
abstract: BACKGROUND
  The availability of fully sequenced genomes and the implementation of transcriptome technologies have increased the studies investigating the expression profiles for a variety of tissues, conditions, and species. In this study, using RNA-seq data for three distinct tissues (brain, liver, and muscle), we investigate how base composition affects mammalian gene expression, an issue of prime practical and evolutionary interest.
  
  
  RESULTS
  We present the transcriptome map of the mouse isochores (DNA segments with a fairly homogeneous base composition) for the three different tissues and the effects of isochores' base composition on their expression activity. Our analyses also cover the relations between the genes' expression activity and their localization in the isochore families.
  
  
  CONCLUSIONS
  This study is the first where next-generation sequencing data are used to associate the effects of both genomic and genic compositional properties to their corresponding expression activity. Our findings confirm previous results, and further support the existence of a relationship between isochores and gene expression. This relationship corroborates that isochores are primarily a product of evolutionary adaptation rather than a simple by-product of neutral evolutionary processes.
authors: Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou, 115 27, Athens, Greece. sarhondakis@bioacademy.gr
authors: Stilianos
authors: S
authors: Arhondakis
authors: Kimon
authors: K
authors: Frousios
authors: Costas S
authors: CS
authors: Iliopoulos
authors: Solon P
authors: SP
authors: Pissis
authors: German
authors: G
authors: Tischler
authors: Sophia
authors: S
authors: Kossida
mesh_terms: D000818:Animals; D001482:Base Composition; D001921:Brain / Q000378:metabolism; D016678:Genome; D032085:Isochores / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D032085:Isochores