title: Genetic variations in plasma circulating DNA of HBV-related hepatocellular carcinoma patients predict recurrence after liver transplantation.
abstract: BACKGROUND
  Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis.
  
  
  METHODS
  Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N = 102 and N = 77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors.
  
  
  RESULTS
  rs894151 and rs12438080 were significantly associated with recurrence (P = .003 and P = .004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P = .040). Similar results were obtained in the third cohort (N = 77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P = .002, respectively).
  
  
  CONCLUSIONS
  Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence.
authors: Liver Cancer Institute, Zhong Shan Hospital, Fudan University, Key Laboratory for Carcinogenesis and Cancer Invasion, the Chinese Ministry of Education, Shanghai Key Laboratory for Organ Transplantation, Shanghai, People's Republic of China.
authors: Jie
authors: J
authors: Hu
authors: Zheng
authors: Z
authors: Wang
authors: Jia
authors: J
authors: Fan
authors: Zhi
authors: Z
authors: Dai
authors: Yi-Feng
authors: YF
authors: He
authors: Shuang-Jian
authors: SJ
authors: Qiu
authors: Xiao-Wu
authors: XW
authors: Huang
authors: Jian
authors: J
authors: Sun
authors: Yong-Sheng
authors: YS
authors: Xiao
authors: Kang
authors: K
authors: Song
authors: Ying-Hong
authors: YH
authors: Shi
authors: Qi-Man
authors: QM
authors: Sun
authors: Xin-Rong
authors: XR
authors: Yang
authors: Guo-Ming
authors: GM
authors: Shi
authors: Lei
authors: L
authors: Yu
authors: Guo-Huan
authors: GH
authors: Yang
authors: Zhen-Bin
authors: ZB
authors: Ding
authors: Qiang
authors: Q
authors: Gao
authors: Zhao-You
authors: ZY
authors: Tang
authors: Jian
authors: J
authors: Zhou
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000821:virology; D004247:DNA / Q000097:blood* / Q000235:genetics*; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D060005:Genotyping Techniques; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000821:virology; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012372:ROC Curve; D012008:Recurrence; D015203:Reproducibility of Results
chemical_list: D004247:DNA