title: Splenic extramedullary hemopoiesis caused by a dysfunctional mutation in the NF-κB-inducing kinase gene.
abstract: NF-κB-inducing kinase (NIK) plays critical roles in the development of lymph nodes and Peyer's patches, and microarchitecture of the thymus and spleen via NF-κB activation. Alymphoplasia (aly/aly) mice have a point mutation in the NIK gene that causes a defect in the activation of an NF-κB member RelB. Here, we developed a novel method to determine the aly mutation by genetic typing using PCR. This method facilitated the easy establishment of a congeneic aly/aly mouse line. Indeed, we generated a mouse line with aly mutation on a BALB/cA background (BALB/cA-aly/aly). BALB/cA-aly/aly mice showed significant splenomegaly with extramedullary hemopoiesis, which was not significant in aly/aly mice on a C57BL/6 background. Interestingly, the splenomegaly and extramedullary hemopoiesis caused by the aly mutation was gender-dependent. These data together with previous reports on extramedullary hemopoiesis in RelB-deficient mice suggest that NIK-RelB signaling may be involved in the suppression of extramedullary hemopoiesis in adult mice.
authors: Division of Cellular and Molecular Biology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo 108-8639, Japan.
authors: Miho
authors: M
authors: Shinzawa
authors: Yuya
authors: Y
authors: Maruyama
authors: Junwen
authors: J
authors: Qin
authors: Nobuko
authors: N
authors: Akiyama
authors: Maki
authors: M
authors: Miyauchi
authors: Hiromi
authors: H
authors: Yanai
authors: Masamichi
authors: M
authors: Takami
authors: Jun-Ichiro
authors: J
authors: Inoue
authors: Taishin
authors: T
authors: Akiyama
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006411:Hematopoiesis, Extramedullary / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D013154:Spleen / Q000502:physiology; D013163:Splenomegaly / Q000235:genetics; D051841:Transcription Factor RelB / Q000235:genetics / Q000378:metabolism*; D000097782:NF-kappaB-Inducing Kinase
chemical_list: D017346:Protein Serine-Threonine Kinases; D051841:Transcription Factor RelB; D000097782:NF-kappaB-Inducing Kinase; C497594:Relb protein, mouse