title: A novel vaccine delivery system: biodegradable nanoparticles in thermosensitive hydrogel.
abstract: In this work, a novel vaccine delivery system, biodegradable nanoparticles (NPs) in thermosensitive hydrogel, was investigated. Human basic fibroblast growth factor (bFGF)-loaded NPs (bFGF-NPs) were prepared, and then bFGF-NPs were incorporated into thermosensitive hydrogel to form bFGF-NPs in a hydrogel composite (bFGF-NPs/hydrogel). bFGF-NPs/hydrogel was an injectable sol at ambient temperature, but was converted into a non-flowing gel at body temperature. The in vitro release profile showed that bFGF could be released from bFGF-NPs or bFGF-NPs/hydrogel at an extended period, but the release rate of bFGF-NPs/hydrogel was much lower. In vivo experiments suggested that immunogenicity of bFGF improved significantly after being incorporated into the NPs/hydrogel composite, and strong humoral immunity was maintained for longer than 12 weeks. Furthermore, an in vivo protective anti-tumor immunity assay indicated that immunization with bFGF-NPs/hydrogel could induce significant suppression of the growth and metastases of tumors. Thus, the NPs/hydrogel composite may have great potential application as a novel vaccine delivery system.
authors: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, and School of Life Sciences, Sichuan University, Chengdu, People's Republic of China.
authors: Qin Jie
authors: QJ
authors: Wu
authors: Xue Chen
authors: XC
authors: Zhu
authors: Xiong
authors: X
authors: Xiao
authors: Pan
authors: P
authors: Wang
authors: Da Ke
authors: da K
authors: Xiong
authors: Chang Yang
authors: CY
authors: Gong
authors: Yong Sheng
authors: YS
authors: Wang
authors: Li
authors: L
authors: Yang
authors: Yu Quan
authors: YQ
authors: Wei
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D018827:Carcinoma, Lewis Lung / Q000276:immunology / Q000517:prevention & control / Q000628:therapy*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D020100:Hydrogels; D051379:Mice; D008810:Mice, Inbred C57BL; D053769:Nanocapsules; D014611:Vaccination / Q000379:methods*
chemical_list: D019496:Cancer Vaccines; D020100:Hydrogels; D053769:Nanocapsules; D016222:Fibroblast Growth Factor 2