title: Knockdown of Ebola virus VP24 impairs viral nucleocapsid assembly and prevents virus replication.
abstract: The structural protein VP24 of Ebola virus (EBOV) is a determinant of virulence in rodent models and possesses an interferon antagonist function. In this study, we investigate the role of VP24 in EBOV replication using RNA interference by small interfering RNA to knock down the expression of this protein in virus-infected cells. We reveal that VP24 is required for assembly of viral nucleocapsids and that silencing of VP24 expression prevents the release of EBOV.
authors: Filovirus Laboratory, INSERM U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
authors: Mathieu
authors: M
authors: Mateo
authors: Caroline
authors: C
authors: Carbonnelle
authors: Miguel J
authors: MJ
authors: Martinez
authors: Olivier
authors: O
authors: Reynard
authors: Audrey
authors: A
authors: Page
authors: Valentina A
authors: VA
authors: Volchkova
authors: Viktor E
authors: VE
authors: Volchkov
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000235:genetics / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D020868:Gene Silencing; D051379:Mice; D019251:Nucleocapsid / Q000502:physiology*; D011817:Rabbits; D011994:Recombinant Proteins; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics* / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; C446938:VP24 protein, Ebola virus; D014764:Viral Proteins