title: Seminal leukocytes and clinical outcomes with donor sperm insemination.
abstract: OBJECTIVE
  To report the level of leukocytospermia in fertile donors' semen. Surprisingly, seminal leukocytes protect fertilization properties of sperm and are associated with normal or improved assisted reproductive technology outcomes in infertility patients. This raises the question of whether leukocytospermia exists in fertile men as well. We report a study of sperm donors who, by law in France, have to be of proven fertility.
  
  
  METHODS
  Retrospective analysis.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  One hundred fifty-five donors were selected for cryobanking. Results of their sperm analyses were compared with those from 10,242 infertile men.
  
  
  METHODS
  None.
  
  
  METHODS
  The men's first ejaculate was studied by cytologic analysis to determine the round cell and polymorphonuclear cell (PMN) contents. A total of 3,875 donor sperm inseminations (DSIs) were performed, and their outcomes were analyzed over an 8-year period.
  
  
  RESULTS
  PMN is more elevated in semen from infertility patients than in semen from fertile donors, but some donors (6.5%) had high leukocytospermia (≥10(6)/mL). The post-DSI pregnancy rate was increased when round cells were present (P<.02) but not with higher PMN concentrations. Furthermore, high leukocytospermia was associated with an increased post-DSI miscarriage rate.
  
  
  CONCLUSIONS
  In fertile donors, as in infertility patients, high leukocytospermia (>10(6)/mL) is associated with a normal pregnancy rate but an increased percentage of early pregnancy loss.
authors: Service d'Histologie-Embryologie-Biologie de la Reproduction, CECOS, Hôpital Cochin, AP-HP, Université Paris Descartes, Paris, France.
authors: Virginie
authors: V
authors: Barraud-Lange
authors: Jean-Christophe
authors: JC
authors: Pont
authors: Khaled
authors: K
authors: Pocate
authors: Jean Marie
authors: JM
authors: Kunstmann
authors: Céline
authors: C
authors: Chalas-Boissonas
authors: Béatrice
authors: B
authors: Ducot
authors: Jean Philippe
authors: JP
authors: Wolf
mesh_terms: D002452:Cell Count; D005260:Female; D006801:Humans; D007246:Infertility / Q000453:epidemiology / Q000628:therapy*; D007316:Insemination, Artificial, Heterologous / Q000706:statistics & numerical data; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology; D018873:Pregnancy Rate; D012189:Retrospective Studies; D012661:Semen / Q000166:cytology* / Q000276:immunology; D055101:Semen Analysis; D013076:Sperm Count; D014019:Tissue Donors; D016896:Treatment Outcome