title: Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli.
abstract: Protein splicing catalyzed by inteins has enabled various biotechnological applications such as protein ligation. Successful applications of inteins are often limited by splicing efficiency. Here, we report the comparison of protein splicing between 20 different inteins from various organisms in identical contexts to identify robust inteins with foreign exteins. We found that RadA intein from Pyrococcus horikoshii and an engineered DnaB intein from Nostoc punctiforme demonstrated an equally efficient splicing activity to the previously reported highly efficient DnaE intein from Nostoc punctiforme. The newly identified inteins with efficient cis-splicing activity can be good starting points for the further development of new protein engineering tools.
authors: Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki. P.O. Box 65, Helsinki, FIN-00014, Finland.
authors: Simo
authors: S
authors: Ellilä
authors: Jaana Maria
authors: JM
authors: Jurvansuu
authors: Hideo
authors: H
authors: Iwaï
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D047669:Exteins; D047668:Inteins / Q000235:genetics; D015202:Protein Engineering; D019154:Protein Splicing / Q000235:genetics; D013696:Temperature
chemical_list: D001426:Bacterial Proteins