title: Quantitative gene expression profiling of CD45+ and CD45- skeletal muscle-derived side population cells.
abstract: The skeletal muscle-derived side population (mSP) which highly excludes Hoechst 33342 is composed of CD45(+) and CD45(-) subpopulations; yet, rareness of mSP cells in general has complicated extensive quantitative analysis of gene expression profiles in primarily isolated mSP cells. Here, we describe the isolation of adult mouse normal skeletal muscle residing SPCD45(+) and SPCD45(-) cells from a parent mononuclear muscle-derived cell (MDC) population. Relative quantitative real time PCR (RT-PCR) of 64 genes revealed that mSPCD45(-) compared with mSPCD45(+) was enriched for cells expressing transcripts associated with endothelial cells, Notch signaling and myogenic precursors. By comparing the mRNA signatures of mSPs with those of adipose tissue-derived SP populations, a common endothelial component seemed to reside in both muscle and fat-derived SPCD45(-) entities. However, each SP subset was clearly specified by the tissue from which the cells originated suggesting that muscle SPs compared with adipose tissue SPs are predisposed towards differentiation into the myogenic lineage. Thus, our data support the previously suggested hypothesis that satellite cell precursors (or alternatively a satellite cell subpopulation) remain in the mSPCD45(-) fraction, and we show that these cells express high levels of many of the known myogenic precursor/stem cell related markers, including Pax7 and Myf5.
authors: Department of Cancer and Inflammation Research, University of Southern Denmark, DK, Denmark.
authors: Ditte Caroline
authors: DC
authors: Andersen
authors: Gitte Qvist
authors: GQ
authors: Kristiansen
authors: Line
authors: L
authors: Jensen
authors: Ernst-Martin
authors: EM
authors: Füchtbauer
authors: Henrik Daa
authors: HD
authors: Schrøder
authors: Charlotte Harken
authors: CH
authors: Jensen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D058985:Side-Population Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D017493:Leukocyte Common Antigens