title: Inflammatory lesions of cochlea in murine cytomegalovirus-infected mice with hearing loss.
abstract: We sought to determine the distribution of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and lymphocytes in the cochlea of mice infected with murine cytomegalovirus (MCMV). For this purpose, 16 newborn mice were divided equally into model and control (uninfected) groups. In model group, 10 μl of MCMV was injected into the brain of each mouse whereas in control group, 10 μl of physiological saline was injected. Fourteen days after the injection of MCMV, the auditory brainstem response audiometry was performed. Later, the mice were killed and the acoustic capsule samples were collected for polymerase chain reaction analysis, histopathological and immunohistochemical studies. Compared with control mice, the incubation period was longer (F = 13.797; P = 0.003) and the amplitude was lower (F = 5.095; P = 0.043) in model group mice. It indicated that the intracerebral injection of MCMV caused hearing loss in mice. Histopathological examination of cochleas revealed increased levels of lymphocytic infiltration in the membrana vestibularis. Higher levels of TNF-α and IL-6 in scala tympani were detected by immunohistochemical staining. Taken together, the hearing loss in mice could be related with the inflammatory changes occurring in cochlea after inception of MCMV infection.
authors: Department of Otolaryngology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors: Qiao
authors: Q
authors: Yuehua
authors: Zhang
authors: Z
authors: Longzhen
authors: Xu
authors: X
authors: Kailin
authors: Zeng
authors: Z
authors: Lingyu
authors: Meng
authors: M
authors: Lingjian
authors: Wang
authors: W
authors: Jun
authors: Meng
authors: M
authors: Hong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D003051:Cochlea / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000378:metabolism; D016057:Evoked Potentials, Auditory, Brain Stem; D015828:Eye Infections, Viral / Q000378:metabolism / Q000473:pathology / Q000821:virology; D034381:Hearing Loss / Q000209:etiology* / Q000473:pathology*; D006566:Herpesviridae Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D015850:Interleukin-6 / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha