title: Antiinflammatory effects of traditional Korean medicine, JinPi-tang and its active ingredient, hesperidin in HaCaT cells.
abstract: A traditional Korean medicine, JinPi-tang (JPT), has been used in dermatological therapeutics and cosmeceuticals including antiaging creams and moisturizers. However, it has not been clarified how JPT and its active ingredient, hesperidin (HES), regulates inflammatory reactions in HaCaT cells. Thus, the mechanisms of action of JPT and HES on inflammatory reactions were investigated for the first time in an experimental model. The antiinflammatory effects of JPT and HES in HaCaT cells were investigated by using an enzyme-linked immunosorbent assay and a reverse transcription-polymerase chain reaction as well as western blot analysis. JPT and HES inhibited the H(2)O(2)-induced interleukin-8 and tumor necrosis factor-α production as well as its mRNA expression. In addition, JPT and HES inhibited the activation of the nuclear factor-κB, the phosphorylation of IκBα and the p38 mitogen-activated protein kinase, as well as the activation of cyclooxygenase-2. The findings suggest that JPT and HES would be helpful in the treatment of UV radiation-induced inflammatory skin diseases.
authors: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors: Phil-Dong
authors: PD
authors: Moon
authors: Hyung-Mim
authors: HM
authors: Kim
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006569:Hesperidin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020672:I-kappa B Proteins / Q000187:drug effects / Q000378:metabolism; D016209:Interleukin-8 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D055670:Medicine, Korean Traditional; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D020672:I-kappa B Proteins; D016209:Interleukin-8; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D006861:Hydrogen Peroxide; D006569:Hesperidin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D048051:p38 Mitogen-Activated Protein Kinases