title: R432 is a key residue for the multiple functions of Ndt80p in Candida albicans.
abstract: Ndt80p is an important transcription modulator to various stress-response genes in Candida albicans, the most common human fungal pathogen in systemic infections. We found that Ndt80p directly regulated its target genes, such as YHB1, via the mid-sporulation element (MSE). Furthermore, the ndt80(R432A) allele, with a reduced capability to bind MSE, failed to complement the defects caused by null mutations of NDT80. Thus, the R432 residue in the Ndt80p DNA-binding domain is involved in all tested functions, including cell separation, drug resistance, nitric oxide inactivation, germ tube formation, hyphal growth, and virulence. Hence, the importance of the R432 residue suggests a novel approach for designing new antifungal drugs by blocking the interaction between Ndt80p and its targets.
authors: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan.
authors: Yun Liang
authors: YL
authors: Yang
authors: Chih Wei
authors: CW
authors: Wang
authors: Shiang Ning
authors: SN
authors: Leaw
authors: Te Pin
authors: TP
authors: Chang
authors: I Chin
authors: IC
authors: Wang
authors: Chia Geun
authors: CG
authors: Chen
authors: Jen Chung
authors: JC
authors: Fan
authors: Kuo Yun
authors: KY
authors: Tseng
authors: Szu Hsuan
authors: SH
authors: Huang
authors: Chih Yu
authors: CY
authors: Chen
authors: Ting Yin
authors: TY
authors: Hsiao
authors: Chao Agnes
authors: CA
authors: Hsiung
authors: Chiung Tong
authors: CT
authors: Chen
authors: Chwan Deng
authors: CD
authors: Hsiao
authors: Hsiu Jung
authors: HJ
authors: Lo
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D025301:Hyphae / Q000254:growth & development; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology*; D014774:Virulence
chemical_list: D004268:DNA-Binding Proteins; C495215:Ndt80 protein, Candida albicans; D014157:Transcription Factors; D009569:Nitric Oxide; D004247:DNA