title: Graphene oxide based photoinduced charge transfer label-free near-infrared fluorescent biosensor for dopamine.
abstract: While the super fluorescence quenching capacity of graphene and graphene oxide (GO) has been extensively employed to develop fluorescent sensors, their own unique fluorescence and its potential for chemo-/biosensing have seldom been explored. Here we report a GO-based photoinduced charge transfer (PCT) label-free near-infrared (near-IR) fluorescent biosensor for dopamine (DA). The multiple noncovalent interactions between GO and DA and the ultrafast decay at the picosecond range of the near-IR fluorescence of GO resulted in effective self-assembly of DA molecules on the surface of GO, and significant fluorescence quenching, allowing development of a PCT-based biosensor with direct readout of the near-IR fluorescence of GO for selective and sensitive detection of DA. The developed method gave a detection limit of 94 nM and a relative standard deviation of 2.0% for 11 replicate detections of 2.0 μM DA and was successfully applied to the determination of DA in biological fluids with quantitative recovery (98-115%).
authors: State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, China.
authors: Jin-Long
authors: JL
authors: Chen
authors: Xiu-Ping
authors: XP
authors: Yan
authors: Kang
authors: K
authors: Meng
authors: Shu-Feng
authors: SF
authors: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D055668:Photochemical Processes; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D013499:Surface Properties
chemical_list: D010087:Oxides; D006108:Graphite; D004298:Dopamine