title: Enzyme activity in dialkyl phosphate ionic liquids.
abstract: The activity of four metagenomic enzymes and an enzyme cloned from the straw mushroom, Volvariella volvacea were studied in the following ionic liquids, 1,3-dimethylimidazolium dimethyl phosphate, [mmim][dmp], 1-ethyl-3-methylimidazolium dimethyl phosphate, [emim][dmp], 1-ethyl-3-methylimidazolium diethyl phosphate, [emim][dep] and 1-ethyl-3-methylimidazolium acetate, [emim][OAc]. Activity was determined by analyzing the hydrolysis of para-nitrobenzene carbohydrate derivatives. In general, the enzymes were most active in the dimethyl phosphate ionic liquids, followed by acetate. Generally speaking, activity decreased sharply for concentrations of [emim][dep] above 10% v/v, while the other ionic liquids showed less impact on activity up to 20% v/v.
authors: Department of Chemistry, Brookhaven National Laboratory, P.O. Box 5000, Upton, NY 11973, USA. mthomas@bnl.gov
authors: Marie F
authors: MF
authors: Thomas
authors: Luen-Luen
authors: LL
authors: Li
authors: Jocelyn M
authors: JM
authors: Handley-Pendleton
authors: Daniel
authors: D
authors: van der Lelie
authors: John J
authors: JJ
authors: Dunn
authors: James F
authors: JF
authors: Wishart
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D052578:Ionic Liquids / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D055367:Volvariella / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D052578:Ionic Liquids; D009943:Organophosphorus Compounds; D001617:beta-Glucosidase; D043364:Endo-1,4-beta Xylanases