title: Changes of thyroid hormone levels and related gene expression in zebrafish on early life stage exposure to triadimefon.
abstract: In this study, zebrafish was exposed to triadimefon. Thyroid hormones levels and the expression of related genes in the hypothalamic-pituitary-thyroid (HPT) axis, including thyroid-stimulating hormone (TSH-beta), deiodinases (dio1 and dio2) and the thyroid hormone receptor (thraa and thrb) were evaluated. After triadimefon exposure, increased T4 can be explained by increased thyroid-stimulating hormone (TSH-beta). The conversion of T4 to T3 (deiodinase type I-dio1) was decreased, which reduced the T3 level. Thyroid hormone receptor beta (thrb) mRNA levels were significantly down-regulated, possibly as a response to the decreased T3 levels. The overall results indicated that triadimefon exposure could alter gene expression in the HPT axis and that mechanisms of disruption of thyroid status by triadimefon could occur at several steps in the synthesis, regulation, and action of thyroid hormones.
authors: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Kaixuan Road 268, Hangzhou, Zhejiang 310029, PR China.
authors: Shaoying
authors: S
authors: Liu
authors: Juhua
authors: J
authors: Chang
authors: Ying
authors: Y
authors: Zhao
authors: Guonian
authors: G
authors: Zhu
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian; D052244:Endocrine Disruptors / Q000633:toxicity; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007031:Hypothalamus / Q000187:drug effects; D007453:Iodide Peroxidase / Q000235:genetics; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects; D013961:Thyroid Gland / Q000187:drug effects; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics; D037042:Thyroid Hormone Receptors beta / Q000235:genetics; D013963:Thyroid Hormones / Q000378:metabolism*; D037322:Thyrotropin, beta Subunit / Q000235:genetics; D014230:Triazoles / Q000633:toxicity*; D014284:Triiodothyronine / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000009:adverse effects; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D052244:Endocrine Disruptors; D037021:Thyroid Hormone Receptors alpha; D037042:Thyroid Hormone Receptors beta; D013963:Thyroid Hormones; D037322:Thyrotropin, beta Subunit; D014230:Triazoles; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D014284:Triiodothyronine; C032910:triadimefon; D007453:Iodide Peroxidase