title: Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients.
abstract: Two commercial polymerase chain reaction (PCR) assays (a real-time PCR [Cepheid] and an oligochromatographic test [Speed-oligo]) and 1 serology test (Serodia-Myco II) for detecting Mycoplasma pneumoniae in nasopharyngeal aspirates and serum samples were studied. Among the 145 samples, 32 serum pairs were serologically positive for M. pneumoniae. Of these, in 30 nasopharyngeal aspirates, M. pneumoniae was detected using the real-time PCR assay and 25 using Speed-oligo, corresponding to a sensitivity of 93.7% and 78.1%, respectively. Among the 94 samples with negative serology, we only obtained 1 positive result by real-time PCR assay. In the group of samples from healthy children, no positive results were obtained.
authors: Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Badalona, Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. sblanc1976@gmail.com
authors: Silvia
authors: S
authors: Blanco
authors: Loreto
authors: L
authors: Fuenzalida
authors: Albert
authors: A
authors: Bas
authors: Cristina
authors: C
authors: Prat
authors: Aida
authors: A
authors: Ramírez
authors: Lurdes
authors: L
authors: Matas
authors: Carlos
authors: C
authors: Rodrigo
authors: Vicente
authors: V
authors: Ausina
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009175:Mycoplasma Infections / Q000175:diagnosis*; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D009305:Nasopharynx / Q000382:microbiology; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D044967:Serum / Q000382:microbiology