title: Kv7 channels can function without constitutive calmodulin tethering.
abstract: M-channels are voltage-gated potassium channels composed of Kv7.2-7.5 subunits that serve as important regulators of neuronal excitability. Calmodulin binding is required for Kv7 channel function and mutations in Kv7.2 that disrupt calmodulin binding cause Benign Familial Neonatal Convulsions (BFNC), a dominantly inherited human epilepsy. On the basis that Kv7.2 mutants deficient in calmodulin binding are not functional, calmodulin has been defined as an auxiliary subunit of Kv7 channels. However, we have identified a presumably phosphomimetic mutation S511D that permits calmodulin-independent function. Thus, our data reveal that constitutive tethering of calmodulin is not required for Kv7 channel function.
authors: Unidad de Biofísica, Consejo Superior de Investigaciones Científicas-Universidad del País Vasco/Euskal Herriko Unibersitatea, Leioa, Spain.
authors: Juan Camilo
authors: JC
authors: Gómez-Posada
authors: Paloma
authors: P
authors: Aivar
authors: Araitz
authors: A
authors: Alberdi
authors: Alessandro
authors: A
authors: Alaimo
authors: Ainhoa
authors: A
authors: Etxeberría
authors: Juncal
authors: J
authors: Fernández-Orth
authors: Teresa
authors: T
authors: Zamalloa
authors: Meritxell
authors: M
authors: Roura-Ferrer
authors: Patricia
authors: P
authors: Villace
authors: Pilar
authors: P
authors: Areso
authors: Oscar
authors: O
authors: Casis
authors: Alvaro
authors: A
authors: Villarroel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002147:Calmodulin / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D051658:KCNQ2 Potassium Channel / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051659:KCNQ3 Potassium Channel / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D017433:Protein Structure, Secondary; D021381:Protein Transport
chemical_list: D002147:Calmodulin; D051658:KCNQ2 Potassium Channel; D051659:KCNQ3 Potassium Channel