title: Development of an HPLC method to analyze and prepare elsinochrome C and hypocrellin A in the submerged fermentation broth of Shiria sp. SUPER-H168.
abstract: A rapid and sensitive analytical method based on reverse-phase high-performance liquid chromatography was first developed to simultaneously determine elsinochrome C (EC) and hypocrellin A (HA) in the submerged fermentation. The mobile phase consisted of acetonitrile-water 60:40 (v/v) with a flow-rate of 1 mL/min. The calibration curves were as follows: y = 37,625x + 249,775 for EC, y = 30,813x + 556,409 for HA and linear at the investigated concentration. The correlation coefficients (R(2) ) were 0.9989 and 0.9998 respectively for EC and HA. The limits of detection and quantification were 175 and 585 µg/L for EC and 205 and 610 µg/L for HA. The precisions of concentration and retention times were less than 2.5 and 0.3%. The recovery of the method was greater than 95.0%. The methodology was applied to analyze simultaneously EC and HA concentrations in a submerged fermentation, and was adequate for analysis of biosynthesis of perylenequinones. The method was also amplified to separate and purify EC and HA using a semi-preparative C(18) column. In addition, elsinochrome C was first identified in the submerged fermentation broth of Shiraia sp. SUPER-H168.
authors: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors: Mingming
authors: M
authors: Hu
authors: Yujie
authors: Y
authors: Cai
authors: Xiangru
authors: X
authors: Liao
authors: Zhikui
authors: Z
authors: Hao
authors: Jiayang
authors: J
authors: Liu
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005285:Fermentation; D057230:Limit of Detection; D016014:Linear Models; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis; D019800:Phenol; D011083:Polycyclic Compounds / Q000032:analysis* / Q000737:chemistry; D011809:Quinones / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D011083:Polycyclic Compounds; D011809:Quinones; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A