title: Haplosporidium nelsoni (MSX) in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China.
abstract: The protozoan parasite Haplosporidium nelsoni (MSX) was identified in Japanese scallops Patinopecten yessoensis (Jay, 1857) from Dalian along the northern coast of the Yellow Sea, China by histopathologic examination, polymerase chain reaction (PCR) amplification, and in situ hybridization (ISH) assay. H. nelsoni plasmodia-like structures were identified in the digestive glands of scallops by histologic examination, but no parasite spores were observed. PCR using the Hap-F2, R2 primer pair produced a sequence with 100% homology with the corresponding small subunit rDNA region of H. nelsoni. An ISH assay using the oligonucleotide probe MSX1347 produced a positive reaction with the Japanese scallop parasite. This is the first report of H. nelsoni in P. yessoensis in China.
authors: Dalian Environmental Monitoring Centre, Dalian, Liaoning 116023, China. wanglaoer1980@hotmail.com
authors: Zhongwei
authors: Z
authors: Wang
authors: Xin
authors: X
authors: Lu
authors: Yubo
authors: Y
authors: Liang
mesh_terms: D000818:Animals; D002681:China; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics; D041602:Haplosporida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D017403:In Situ Hybridization; D049897:Pectinidae / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D033761:Spores, Protozoan
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan