title: Alignment of human vascular smooth muscle cells on parallel electrospun synthetic elastin fibers.
abstract: We generated parallel elastic fibers from synthetic elastin (SE) as a model of the arterial media and assessed the alignment of smooth muscle cells (SMCs). SE utilized crosslinked electrospun human tropoelastin to form aligned fibers that mimicked the topography and elastin-rich content of the medial extracellular matrix. Bundled parallel fibers were anisotropically more elastic than randomly arranged scaffolds (111 ± 25 kPa vs. 265 ± 17 kPa) in the direction of the fibers. Aligned and random fiber scaffolds each supported SMC growth. Following attachment, SMCs proliferated longitudinally on the parallel fibers and expressed native α-smooth muscle actin.
authors: School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
authors: Lisa
authors: L
authors: Nivison-Smith
authors: Anthony S
authors: AS
authors: Weiss
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D001696:Biomechanical Phenomena / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002452:Cell Count; D002478:Cells, Cultured; D004549:Elastin / Q000494:pharmacology* / Q000648:ultrastructure; D005583:Fourier Analysis; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014333:Tropoelastin / Q000494:pharmacology / Q000648:ultrastructure
chemical_list: D014333:Tropoelastin; D004549:Elastin