title: Effect of trichlorfon on oxidative stress and hepatocyte apoptosis of Carassius auratus gibelio in vivo.
abstract: To investigate the effect of trichlorfon on oxidative stress and hepatocyte apoptosis of Carassius auratus gibelio in vivo, the fish were exposed to 0, 0.5, 1.0, 2.0 and 4.0 mg l(-1) trichlorfon concentrations for 30 days. At the end of the experiment, plasm antioxidant activities, hepatic total nitric oxide synthase (T-NOS) activity, xanthine oxidase (XOD) activity and hepatocyte apoptosis rate were analyzed. The results showed hepatic T-NOS activities, XOD activities and hepatocyte apoptosis rate were increased with trichlorfon concentration increase. The hepatic MDA content was increased significantly at 4.0 mg l(-1) trichlorfon treatment. The disturbance of antioxidative balance was observed based on the monitoring of the plasm superoxide dismutase (SOD), catalase (CAT) activities and vitamin E levels. The current study revealed that trichlorfon influenced fish plasma antioxidative status, caused lipid peroxidation (LPO) then resulted in hepatocytes apoptosis.
authors: Key Lab of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: WeiNa
authors: W
authors: Xu
authors: WenBin
authors: W
authors: Liu
authors: KangLe
authors: K
authors: Lu
authors: YangYang
authors: Y
authors: Jiang
authors: GuiFeng
authors: G
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002374:Catalase / Q000097:blood; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010575:Pesticides / Q000633:toxicity; D013482:Superoxide Dismutase / Q000097:blood; D014236:Trichlorfon / Q000633:toxicity*; D014810:Vitamin E / Q000097:blood; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D010575:Pesticides; D014810:Vitamin E; D008315:Malondialdehyde; D014236:Trichlorfon; D002374:Catalase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase