title: The G protein coupled receptor Gpr153 shares common evolutionary origin with Gpr162 and is highly expressed in central regions including the thalamus, cerebellum and the arcuate nucleus.
abstract: The Rhodopsin family of G protein coupled receptors (GPCRs) includes the phylogenetic α-group consisting of about 100 human members. The α-group is the only group of GPCRs that has many receptors for biogenic amines which are major drug targets. Several members of this group are orphan receptors and their functions are elusive. In this study we present a detailed phylogenetic and anatomical characterization of the Gpr153 receptor and also attempt to study its functional role. We identified the homologue of Gpr153 in the elephant shark genome and phylogenetic and synteny analyses revealed that Gpr162 and Gpr153 share a common ancestor that split most likely through a duplication event before the divergence of the tetrapods and the teleost lineage. A quantitative real-time PCR study reveals widespread expression of Gpr153 in the central nervous system and all the peripheral tissues investigated. Detailed in situ hybridization on mouse brain showed specifically high expression in the thalamus, cerebellum and the arcuate nucleus. The antisense oligodeoxynucleotide knockdown of Gpr153 caused a slight reduction in food intake and the elevated plus maze test showed significant reduction in the percentage of time spent in the centre square, which points towards a probable role in decision making. This report provides the first detailed characterization of the evolution, expression and primary functional properties of the Gpr153 gene.
authors: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors: Smitha
authors: S
authors: Sreedharan
authors: Markus S
authors: MS
authors: Almén
authors: Valeria P
authors: VP
authors: Carlini
authors: Tatjana
authors: T
authors: Haitina
authors: Olga
authors: O
authors: Stephansson
authors: Wolfgang H
authors: WH
authors: Sommer
authors: Markus
authors: M
authors: Heilig
authors: Susana R
authors: SR
authors: de Barioglio
authors: Robert
authors: R
authors: Fredriksson
authors: Helgi B
authors: HB
authors: Schiöth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism*; D001522:Behavior, Animal / Q000187:drug effects; D002531:Cerebellum / Q000378:metabolism*; D004435:Eating; D019143:Evolution, Molecular; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D012754:Sharks / Q000235:genetics; D026801:Synteny; D013788:Thalamus / Q000378:metabolism*
chemical_list: C569436:Gpr153 protein, human; C569435:Gpr162 protein, human; D043562:Receptors, G-Protein-Coupled