title: Label-free solution-based kinetic study of aptamer-small molecule interactions by kinetic capillary electrophoresis with UV detection revealing how kinetics control equilibrium.
abstract: Here we demonstrate a label-free solution-based approach for studying the kinetics of biopolymer-small molecule interactions. The approach utilizes kinetic capillary electrophoresis (KCE) separation and UV light absorption detection of the unlabeled small molecule. In this proof-of-concept work, we applied KCE-UV to study kinetics of interaction between a small molecule and a DNA aptamer. From the kinetic analysis of a series of aptamers, we found that dissociation rather than binding controls the stability of the complex. Because of its label-free features and generic nature, KCE-UV promises to become a practical tool for challenging kinetic studies of biopolymer-small molecule interactions.
authors: Jiayin
authors: J
authors: Bao
authors: Svetlana M
authors: SM
authors: Krylova
authors: Oren
authors: O
authors: Reinstein
authors: Philip E
authors: PE
authors: Johnson
authors: Sergey N
authors: SN
authors: Krylov
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007700:Kinetics; D011803:Quinine / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D052157:Aptamers, Nucleotide; D012996:Solutions; D011803:Quinine