title: Three dimension optical imaging in a high throughput layered expression system.
abstract: Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.
authors: Department of Biomedical Engineering, The Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, Israel.
authors: Reut
authors: R
authors: Cohen
authors: Gallya
authors: G
authors: Gannot
authors: Moshe
authors: M
authors: Ben David
authors: Nir
authors: N
authors: Sochen
authors: Israel
authors: I
authors: Gannot
mesh_terms: D000362:Agar / Q000737:chemistry*; D000465:Algorithms; D002138:Calibration; D003198:Computer Simulation; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D005782:Gels; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods; D055095:Optics and Photonics / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012984:Software
chemical_list: D005456:Fluorescent Dyes; D005782:Gels; D010455:Peptides; D000362:Agar