title: A TetR-like regulator broadly affects the expressions of diverse genes in Mycobacterium smegmatis.
abstract: Transcriptional regulation plays a critical role in the life cycle of Mycobacterium smegmatis and its related species, M. tuberculosis, the causative microbe for tuberculosis. However, the key transcriptional factors involved in broad regulation of diverse genes remain to be characterized in mycobacteria. In the present study, a TetR-like family transcriptional factor, Ms6564, was characterized in M. smegmatis as a master regulator. A conserved 19 bp-palindromic motif was identified for Ms6564 binding using DNaseI footprinting and EMSA. A total of 339 potential target genes for Ms6564 were further characterized by searching the M. smegmatis genome based on the sequence motif. Notably, Ms6564 bound with the promoters of 37 cell cycle and DNA damage/repair genes and regulated positively their expressions. The Ms6564-overexpressed recombinant strain yielded 5-fold lower mutation rates and mutation frequencies, whereas deletion of Ms6564 resulted in ∼5-fold higher mutation rates for the mutant strain compared with the wild-type strain. These findings suggested that Ms6564 may function as a global regulator and might be a sensor necessary for activation of DNA damage/repair genes.
authors: National Key Laboratory of Agricultural Microbiology, Center for Proteomics Research, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors: Min
authors: M
authors: Yang
authors: Chunhui
authors: C
authors: Gao
authors: Tao
authors: T
authors: Cui
authors: Jingning
authors: J
authors: An
authors: Zheng-Guo
authors: ZG
authors: He
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004249:DNA Damage; D004260:DNA Repair; D015964:Gene Expression Regulation, Bacterial; D018816:Genes, cdc; D055029:Inverted Repeat Sequences; D009154:Mutation; D059645:Mutation Rate; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000378:metabolism; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D011938:Rec A Recombinases / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; D011938:Rec A Recombinases