title: Effects of kallikrein-related peptidase 14 gene inhibition by small             interfering RNA in ovarian carcinoma cells.
abstract: Kallikrein-related peptidase 14 (KLK14) is a member of the tissue kallikrein family of proteases, which are associated with the pathogenesis of malignant tumors and are over-expressed in ovarian carcinoma. However, the mechanism through which KLK14 is implicated in ovarian cancer remains unclear. The aim of the present study was to investigate the effects of KLK14 gene inhibition by small interfering RNA (siRNA) on the growth, apoptosis and invasion of ovarian carcinoma cells in vitro. KLK14 siRNA was transiently transfected into SK-OV-3 and OVCAR-3 ovarian carcinoma cells for 48 h. The expression of KLK14 was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Cell proliferation, apoptosis and invasion were examined by MTT, flow cytometry and Matrigel assay, respectively. The expression of survivin, caspase 9, cleaved caspase 3 and MMP2 protein was measured by Western blot analysis. The expression of KLK14 was significantly downregulated by siRNA in SK-OV-3 and OVCAR-3 cells at both the mRNA and protein levels. Following transfection with KLK14 siRNA, cell growth and invasion were significantly suppressed, and cell apoptosis was markedly induced. The expression of survivin and MMP2 was decreased, while the espression of caspase 9 and cleaved caspase 3 was increased. These results indicate that KLK14 is implicated in the malignant behavior of ovarian carcinoma cells in vitro, and that KLK14 may serve as a target for therapy of ovarian carcinoma.
authors: Department of Obstetrics and Gynaecology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, PR China.
authors: Ruitao
authors: R
authors: Zhang
authors: Huirong
authors: H
authors: Shi
authors: Zhimin
authors: Z
authors: Chen
authors: Wei
authors: W
authors: Feng
authors: Hailing
authors: H
authors: Zhang
authors: Kaiyuan
authors: K
authors: Wu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D007610:Kallikreins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; C425336:KLK14 protein, human; D007610:Kallikreins; D053148:Caspase 3; D053453:Caspase 9; D020778:Matrix Metalloproteinase 2