title: PDZ binding of TARPγ-8 controls synaptic transmission but not synaptic plasticity.
abstract: The reduction in synaptic transmission and plasticity in mice lacking the hippocampus-enriched AMPA receptor (AMPAR) auxiliary subunit TARPγ-8 could be a result of a reduction in AMPAR expression or of the direct action of γ-8. We generated TARPγ-8Δ4 knock-in mice lacking the C-terminal PDZ ligand. We found that synaptic transmission and AMPARs were reduced in the mutant mice, but extrasynaptic AMPAR expression and long-term potentiation (LTP) were unaltered. Our findings suggest that there are distinct TARP-dependent mechanisms for synaptic transmission and LTP.
authors: Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut, USA.
authors: Akio
authors: A
authors: Sumioka
authors: Travis E
authors: TE
authors: Brown
authors: Akihiko S
authors: AS
authors: Kato
authors: David S
authors: DS
authors: Bredt
authors: Julie A
authors: JA
authors: Kauer
authors: Susumu
authors: S
authors: Tomita
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001703:Biophysics; D015220:Calcium Channels / Q000235:genetics; D000076122:Disks Large Homolog 4 Protein; D004558:Electric Stimulation; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D051528:Guanylate Kinases / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D054731:PDZ Domains / Q000235:genetics / Q000502:physiology*; D018408:Patch-Clamp Techniques; D009435:Synaptic Transmission / Q000235:genetics / Q000502:physiology*; D016708:Synaptophysin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
chemical_list: C113940:Cacng2 protein, mouse; D015220:Calcium Channels; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D008565:Membrane Proteins; D016708:Synaptophysin; C515550:TARP gamma-8 protein, mouse; D051528:Guanylate Kinases