title: The reaction of H(2)S with oxidized thiols: generation of persulfides and implications to H(2)S biology.
abstract: Hydrogen sulfide is an endogenously generated molecule with many reported physiological functions. Although several biological targets have been proposed, the biochemical mechanisms by which it elicits activity are not established. Thus, in an effort to begin to delineate the fundamental biological chemistry of H(2)S, we have examined the reaction of H(2)S with oxidized thiols and thiol proteins in order to determine whether persulfide formation occurs, is stable and how this may affect protein function. We have found that persulfides are easily generated, relatively stable and can alter enzyme activity. Moreover, we have begun to develop methodology for in situ generation of persulfides to facilitate further study of this potentially important species.
authors: Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
authors: Nestor E
authors: NE
authors: Francoleon
authors: Samantha J
authors: SJ
authors: Carrington
authors: Jon M
authors: JM
authors: Fukuto
mesh_terms: D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D004228:Dithionitrobenzoic Acid / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010206:Papain / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D013438:Sulfhydryl Compounds; D013440:Sulfides; C051552:persulfides; D045304:Cytochromes c; D004228:Dithionitrobenzoic Acid; D010206:Papain; D019803:Glutathione Disulfide; D006862:Hydrogen Sulfide