title: Unconventional secretion of Ebola virus matrix protein VP40.
abstract: The Ebola virus matrix protein VP40 plays an essential role in virus assembly and budding. In this study we reveal that transient VP40 expression results in the release into the culture medium of substantial amounts of soluble monomeric VP40 in addition to the release of virus-like particles containing an oligomeric form of this protein as previously described. We show that VP40 secretion is endoplasmic reticulum/Golgi-independent and is not associated with cell death. Soluble VP40 was observed during Ebola virus infection of cells and was also found in the serum of virus-infected animals albeit in lower amounts. Unconventional secretion of VP40 may therefore play a role in Ebola virus pathogenicity.
authors: Filovirus Laboratory, Inserm U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
authors: Olivier
authors: O
authors: Reynard
authors: St Patrick
authors: SP
authors: Reid
authors: Audrey
authors: A
authors: Page
authors: Mathieu
authors: M
authors: Mateo
authors: Nathalie
authors: N
authors: Alazard-Dany
authors: Hervé
authors: H
authors: Raoul
authors: Christopher F
authors: CF
authors: Basler
authors: Viktor E
authors: VE
authors: Volchkov
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003470:Culture Media; D029043:Ebolavirus / Q000378:metabolism* / Q000472:pathogenicity; D004721:Endoplasmic Reticulum; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006056:Golgi Apparatus; D057809:HEK293 Cells; D006801:Humans; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins