title: Cell-free synthesis, reconstitution, and characterization of a mitochondrial dicarboxylate-tricarboxylate carrier of Plasmodium falciparum.
abstract: The malaria parasite, Plasmodium falciparum, was recently shown to operate a branched pathway of tricarboxylic acid (TCA) metabolism. To identify and characterize membrane transporters required for such TCA metabolism in the parasite, we isolated a cDNA for a dicarboxylate-tricarboxylate carrier homolog (PfDTC), synthesized the encoded protein with the use of a cell-free translation system, and determined the substrate specificity of its transport activity with a proteoliposome reconstitution system. PfDTC was found to mediate efficient oxoglutarate-malate, oxoglutarate-oxaloacetate, or oxoglutarate-oxoglutarate exchange across the liposome membrane. Our results suggest that PfDTC may mediate the oxoglutarate-malate exchange across the inner mitochondrial membrane required for the branched pathway of TCA metabolism in the malaria parasite.
authors: Cell-Free Science and Technology Research Center and Venture Business Laboratory, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan.
authors: Akira
authors: A
authors: Nozawa
authors: Ryoji
authors: R
authors: Fujimoto
authors: Hiroki
authors: H
authors: Matsuoka
authors: Takafumi
authors: T
authors: Tsuboi
authors: Yuzuru
authors: Y
authors: Tozawa
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D002474:Cell-Free System / Q000737:chemistry; D029382:Dicarboxylic Acid Transporters / Q000096:biosynthesis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism*; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D014233:Tricarboxylic Acids / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D029382:Dicarboxylic Acid Transporters; D015800:Protozoan Proteins; D014233:Tricarboxylic Acids; C020723:citrate-binding transport protein