title: Loss of heterozygosity on chromosome 5 in Iranian  esophageal cancer patients.
abstract: There is a high incidence of esophageal squamous cell  carcinoma (ESCC) in Iran. Non-functionality of some tumor suppressor genes has  been reported in esophageal cancer. Loss of heterozygosity on chromosome 5 has  also been reported in esophageal carcinomas. We assessed loss of heterozygosity  along a region of the long arm of chromosome 5 (5q), from 5q23.1 to 5q23.2, by  PCR amplifying DNA fragments of tumor tissues from patients with ESCC and their  corresponding normal samples. The PCR products were electrophoresed on 6%  non-denaturing polyacrylamide gels, and band intensity was shown by silver  staining. Of 40 patients with ESCC, 27, 25 and 36% of informative cases showed  allelic losses at microsatellite markers D5S1384, D5S1478 and D5S1505,  respectively. Two of the 40 patients studied had microsatellite instability at  marker D5S1384. Based on the fact that loss of heterozygosity with more than  22% incidence for a specific marker cannot be regarded as a random event, we  add support to previous reports concerning the presence of tumor suppressor  genes in this chromosome region and that they affect esophageal cancer development.  According to the data in NCBI UniSTS, the PCR product size of human DNA with  primers of the D5S1505 marker ranges from 243 to 275 bp, containing about 20  repeats of the TAGA tetranucleotide, while the amplicon size of one allele of  one of our cases was 207 bp, with about 10 repeats of the TAGA tetranucleotide,  which would be the shortest sequence reported so far.
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: F
authors: F
authors: Attaran-Bandarabadi
authors: A A
authors: AA
authors: Ziaee
authors: M
authors: M
authors: Yazdanbod
authors: M
authors: M
authors: Shahpanah
authors: A
authors: A
authors: Setayeshgar
authors: M
authors: M
authors: Nassiri
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D007492:Iran; D019656:Loss of Heterozygosity; D008297:Male; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged