title: [Mannose-binding lectin (MBL) inhibits human cytomegalovirus infection of human MD-DC].
abstract: OBJECTIVE
  To explore the inhibitory effect of different sources, different concentrations of Mannose-binding lectin (MBL) on human cytomegalovirus infection of human MD-DC cells.
  
  
  METHODS
  The recombinant MBL was acquired by vector construction, and the natural MBL was purified from human plamsa. MD-DC were pre-exposed to several dilutions of the hMBL/rMBL for 30 min, then HCMV suspensions were added to MD-DC for 2 h to compare the inhibitory effect of hMBL/rMBL on the HCMV infection of MD-DC. MD-DC infected by HCMV co-culture with hMBL/rMBL to compare the inhibitory effect of hMBL/rMBL on the HCMV diffusion between MD-DC. HCMV-DNA in MD-DC was detected by fluorescence quantitative PCR. HCMV-PP65 in MD-DC was analyzed with flow cytometry, the ability of MD-DC to capture HCMV was observed with immunofluorescence confocal microscope.
  
  
  RESULTS
  In hMBL/rMBL inhibition the ability of MD-DC capture HCMV experiments, the fluorescent quantitative PCR demonstrated that the amount of HCMV-DNA in 1 microg/mL of hMBL/rMBL treated cells was not significantly different from that of control group (P < 0.05). But the HCMV-DNA in 5 microg/mL and 10 microg/mL hMBL/rMBL treated group were significantly lower than that of control group (P < 0.05). The significant inhibit effects of 10 microg/mL hMBL/rMBL on the ability of MD-DC capture HCMV were observed by immunofluorescence confocal microscopy and flow cytometry. The inhibit effects of hMBL/rMBL on HCMV diffusion between MD-DC were also observed in 5 microg/mL and 10 microg/mL hMBL/rMBL treated groups at 72 hours.
  
  
  CONCLUSIONS
  The hMBL/rMBL in physiological concentration range (5-10 microg/mL) can significantly inhibit human cytomegalovirus infection of human MD-DC cells, and the hMBL is more effective than rMBL.
authors: Center Laboratory, Affiliated Children's Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. shangsq33@sina.com
authors: Kai-Yu
authors: KY
authors: Pan
authors: Shi-Qiang
authors: SQ
authors: Shang
authors: Ying-Hu
authors: YH
authors: Chen
authors: Wei
authors: W
authors: Wu
authors: Hui-Ju
authors: HJ
authors: Qiao
authors: Ran
authors: R
authors: Tao
mesh_terms: D003587:Cytomegalovirus / Q000187:drug effects*; D003586:Cytomegalovirus Infections / Q000517:prevention & control*; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D006801:Humans; D037601:Mannose-Binding Lectin / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D037601:Mannose-Binding Lectin; D011994:Recombinant Proteins