title: Molecularly imprinted polymer for selective extraction of 3-methylflavone-8-carboxylic acid from human urine followed by its determination using zwitterionic hydrophilic interaction liquid chromatography.
abstract: A novel water-compatible molecularly imprinted SPE combined with zwitterionic hydrophilic interaction liquid chromatography method for selective extraction and determination of 3-methylflavone-8-carboxylic acid (MFA), the main active metabolite of flavoxate in human urine, was developed and validated. The effects of progenic solvents, pH, cross linker and amount of monomer were studied to optimize the efficiency and selectivity. The molecularly imprinted polymer showed good specific adsorption capacity with an optimum of 200 μmol/g at pH 7.5 and selective extraction of MFA from human urine. The recovery of MFA from human urine was >98%. The lower limit of quantification was 1.20 μg/mL. The proposed method overcomes the matrix effects of endogenous substances generally encountered during direct analysis of urine sample.
authors: HPLC Group, Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad, Andhra Pradesh, India. rnrao@iict.res.in
authors: Ramisetti N
authors: RN
authors: Rao
authors: Pawan K
authors: PK
authors: Maurya
authors: Ramakrishna
authors: R
authors: Kuntamukkala
authors: Wani D
authors: WD
authors: Vitthal
authors: Murali V N K
authors: MV
authors: Talluri
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D005422:Flavoxate / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D011108:Polymers; D005422:Flavoxate; C011504:3-methylflavone-8-carboxylic acid