title: Scorpion and spider venom peptides: gene cloning and peptide expression.
abstract: This communication reviews most of the important findings related to venom components isolated from scorpions and spiders, mainly by means of gene cloning and expression. Rather than revising results obtained by classical biochemical studies that report structure and function of venom components, here the emphasis is placed on cloning and identification of genes present in the venomous glands of these arachnids. Aspects related to cDNA library construction, specific or random ESTs cloning, transcriptome analysis, high-throughput screening, heterologous expression and folding are briefly discussed, showing some numbers of species and components already identified, but also shortly mentioning limitations and perspectives of research for the future in this field.
authors: Instituto de Biotecnología - UNAM, Avenida Universidad, Colonia Chamilpa, Cuernavaca, Morelos, Mexico.
authors: V
authors: V
authors: Quintero-Hernández
authors: E
authors: E
authors: Ortiz
authors: M
authors: M
authors: Rendón-Anaya
authors: E F
authors: EF
authors: Schwartz
authors: B
authors: B
authors: Becerril
authors: G
authors: G
authors: Corzo
authors: L D
authors: LD
authors: Possani
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags / Q000737:chemistry / Q000378:metabolism; D020869:Gene Expression Profiling; D015723:Gene Library; D057166:High-Throughput Screening Assays; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D013111:Spider Venoms / Q000737:chemistry / Q000235:genetics*
chemical_list: D010455:Peptides; D012604:Scorpion Venoms; D013111:Spider Venoms