title: Au-nanoclusters incorporated 3-amino-5-mercapto-1,2,4-triazole film modified electrode for the simultaneous determination of ascorbic acid, dopamine, uric acid and nitrite.
abstract: A novel biosensor has been constructed by the electrodeposition of Au-nanoclusters (nano-Au) on poly(3-amino-5-mercapto-1,2,4-triazole) (p-TA) film modified glassy carbon electrode (GCE) and employed for the simultaneous determination of dopamine (DA), ascorbic acid (AA), uric acid (UA) and nitrite (NO(2)(-)). NH(2) and SH groups exposed to the p-TA layer are helpful for the electrodeposition of nano-Au. The combination of nano-Au and p-TA endow the biosensor with large surface area, good biological compatibility, electricity and stability, high selectivity and sensitivity and flexible and controllable electrodeposition process. In the fourfold co-existence system, the linear calibration plots for AA, DA, UA and NO(2)(-) were obtained over the range of 2.1-50.1 μM, 0.6-340.0 μM, 1.6-110.0 μM and 15.9-277.0 μM with detection limits of 1.1×10(-6) M, 5.0×10(-8) M, 8.0×10(-8) M and 8.9×10(-7) M, respectively. In addition, the modified biosensor was applied to the determination of AA, DA, UA and NO(2)(-) in urine and serum samples by using standard adding method with satisfactory results.
authors: Key Laboratory of Analytical Chemistry (Chongqing), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Cun
authors: C
authors: Wang
authors: Ruo
authors: R
authors: Yuan
authors: Yaqin
authors: Y
authors: Chai
authors: Yu
authors: Y
authors: Zhang
authors: Fangxin
authors: F
authors: Hu
authors: Meihe
authors: M
authors: Zhang
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009573:Nitrites / Q000032:analysis*; D014527:Uric Acid / Q000032:analysis*
chemical_list: D045424:Complex Mixtures; D009573:Nitrites; D014527:Uric Acid; D006046:Gold; D001205:Ascorbic Acid; D004298:Dopamine