title: Infectious bursal disease DNA vaccination conferring protection by delayed appearance and rapid clearance of invading viruses.
abstract: The present study was undertaken to determine the kinetics of viral load and immune response in protection against infectious bursal disease virus (IBDV) by DNA vaccination. Chickens were DNA-vaccinated and challenged with IBDV one week after the third vaccination. Tissues were collected at 12 hours postinfection (HPI), 1 day postinfection (DPI), 3, 5, 7 and 10 DPI. The vaccinated chickens had less viral RNA, with delayed appearance and shorter duration in the bursa of Fabricius, spleen, and cecal tonsil than the challenged control chickens. Their ELISA and neutralizing antibody titers were decreased at 12 HPI and significantly lower (P < 0.05) than those in the challenged control chickens at later time points. Their spleen IFNγ expression was up-regulated compared to that in the DNA-vaccinated chickens without IBDV challenge. These results indicate that DNA vaccination confers protection against IBDV challenge by delayed appearance and rapid clearance of the invading viruses.
authors: Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.
authors: Yung-Yi
authors: YY
authors: Chen
authors: Ming Kun
authors: MK
authors: Hsieh
authors: Chun-Yu
authors: CY
authors: Tung
authors: Ching Ching
authors: CC
authors: Wu
authors: Tsang Long
authors: TL
authors: Lin
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D030161:Avian Proteins / Q000235:genetics; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D002060:Bursa of Fabricius / Q000276:immunology / Q000821:virology; D002645:Chickens; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007371:Interferon-gamma / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D013154:Spleen / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D019444:Vaccines, DNA / Q000494:pharmacology; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D030161:Avian Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma