title: Autofluorescence imaging and the quantitative intensity of fluorescence for evaluating the dysplastic grade of colonic neoplasms.
abstract: OBJECTIVE
  Autofluorescence imaging (AFI) is a novel technology which can capture fluorescence emitted from intestinal tissues. While AFI is useful for detecting colorectal neoplasms, it is unclear whether AFI can facilitate the diagnosis by differentiating the extent of dysplasia of colorectal neoplasms. This study investigated the efficacy of AFI in discriminating high-grade from low-grade adenoma.
  
  
  METHODS
  Sixty-seven patients who underwent colonoscopy with AFI were enrolled in this study. The AFI images obtained from 158 lesions in these patients were visually classified into four categories, namely, green (G), green with magenta spots (GM), magenta with green spots (MG), and magenta (M), according to their color intensities, immediately after the examination. The AFI images of the lesions were quantified using an image-analytical software program (F index). Either the F index or the visual assessment was prospectively compared with the dysplastic grade.
  
  
  RESULTS
  The F index of the high-grade adenomas was significantly lower than that of the low-grade adenomas, hyperplasia, and normal mucosa (p < 0.05). The incidence of the lesions classified into the M classification for high-grade adenomas (55.6%) was significantly higher than that of either low-grade adenomas (20.8%) or hyperplasia (0%). No correlation was observed between the F index or the visual classification and the tumor shape. The F index was not influenced by the size of the lesion, while the size was significantly associated with the visual classification of AFI.
  
  
  CONCLUSIONS
  AFI, particularly the F index, is considered to be a useful procedure for estimating the dysplastic grade of colonic adenomas.
authors: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1, Midorigaoka-higashi, Asahikawa, Hokkaido, 078-8510, Japan.
authors: Kentaro
authors: K
authors: Moriichi
authors: Mikihiro
authors: M
authors: Fujiya
authors: Ryu
authors: R
authors: Sato
authors: Toshie
authors: T
authors: Nata
authors: Yoshiki
authors: Y
authors: Nomura
authors: Nobuhiro
authors: N
authors: Ueno
authors: Chisato
authors: C
authors: Ishikawa
authors: Yuhei
authors: Y
authors: Inaba
authors: Takahiro
authors: T
authors: Ito
authors: Kotaro
authors: K
authors: Okamoto
authors: Hiroki
authors: H
authors: Tanabe
authors: Yusuke
authors: Y
authors: Mizukami
authors: Jiro
authors: J
authors: Watari
authors: Yusuke
authors: Y
authors: Saitoh
authors: Yutaka
authors: Y
authors: Kohgo
mesh_terms: D000236:Adenoma / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology*; D003113:Colonoscopy; D005453:Fluorescence; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D011237:Predictive Value of Tests; D011446:Prospective Studies; D018709:Statistics, Nonparametric