title: A common structural blueprint for plant UDP-sugar-producing pyrophosphorylases.
abstract: Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common structural blueprint. Their structures are characterized by the presence of the Rossmann fold in the central (catalytic) domain linked to enzyme-specific N-terminal and C-terminal domains, which may play regulatory functions. Molecular mobility between these domains plays an important role in substrate binding and catalysis. Evolutionary relationships and the role of (de)oligomerization as a regulatory mechanism are discussed.
authors: Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Sweden. leszek.kleczkowski@plantphys.umu.se
authors: Leszek A
authors: LA
authors: Kleczkowski
authors: Matt
authors: M
authors: Geisler
authors: Elisabeth
authors: E
authors: Fitzek
authors: Malgorzata
authors: M
authors: Wilczynska
mesh_terms: D000818:Animals; D006801:Humans; D009713:Nucleotidyltransferases / Q000096:biosynthesis* / Q000737:chemistry* / Q000502:physiology; D010802:Phylogeny; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000502:physiology; D040681:Structural Homology, Protein; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000096:biosynthesis / Q000737:chemistry / Q000502:physiology; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis* / Q000737:chemistry* / Q000502:physiology
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D014539:Uridine Diphosphate Sugars; D009713:Nucleotidyltransferases; C023226:UDPacetylglucosamine pyrophosphorylase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase