title: Sperm transcriptome profiling in oligozoospermia.
abstract: OBJECTIVE
  Investigate in what extent sperm transcriptome of infertile men is different from that of fertile individuals.
  
  
  METHODS
  Semen samples were collected for determination of sperm parameters as well as for RNA isolation. Gene expression profile was investigated in spermatozoa of 8 infertile and 3 fertile men by microarray analysis using the Affymetrix Chip HG-U133 Plus 2.0.
  
  
  RESULTS
  We observed up to 33-fold reduction expression of genes involved in spermatogenesis and sperm motility. Furthermore, there is an important decrease in expression of genes involved in DNA repair as well as oxidative stress regulation. In this study, we also show a striking drop in expression of histone modification genes.
  
  
  CONCLUSIONS
  We found that transcription profile in germ cells of men with idiopathic infertility is different from that of fertile individuals. Interestingly, about 15% of the regulated genes (Eddy Rev Reprod 4:23-30, 1999) play a role in spermatogenesis.
authors: Advanced Technology Laboratory, ZA de l'Agiot 4 rue Louis Lormand, 78320, La Verrière, Paris, France. debbie_montjean@hotmail.com
authors: Debbie
authors: D
authors: Montjean
authors: Pierre
authors: P
authors: De La Grange
authors: David
authors: D
authors: Gentien
authors: Audrey
authors: A
authors: Rapinat
authors: Stéphanie
authors: S
authors: Belloc
authors: Paul
authors: P
authors: Cohen-Bacrie
authors: Yves
authors: Y
authors: Menezo
authors: Moncef
authors: M
authors: Benkhalifa
mesh_terms: D004260:DNA Repair / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009845:Oligospermia / Q000235:genetics* / Q000473:pathology; D018384:Oxidative Stress / Q000235:genetics; D013081:Sperm Motility / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics