title: Efficient conversion of sugarcane stalks into ethanol employing low temperature alkali pretreatment method.
abstract: An alternative route for bio-ethanol production from sugarcane stalks (juice and bagasse) featuring a previously reported low temperature alkali pretreatment method was evaluated. Test-tube scale pretreatment-saccharification experiments were carried out to determine optimal LTA pretreatment conditions for sugarcane bagasse with regard to the efficiency of enzymatic hydrolysis of the cellulose. Free fermentable sugars and bagasse recovered from 2 kg of sugarcane stalks were jointly converted into ethanol via separate enzymatic hydrolysis and fermentation (SHF). Results showed that 98% of the cellulose present in the optimally pretreated bagasse was hydrolyzed into glucose after 72-h enzymatic saccharification using commercially available cellulase and β-glucosidase preparations at relatively low enzyme loading. The fermentable sugars in the mixture of the sugar juice and the bagasse hydrolysate were readily converted into 193.5 mL of ethanol by Saccharomyces cerevisiae within 12h, achieving 88% of the theoretical yield from the sugars and cellulose.
authors: Carbohydrate Laboratory, Food Resource Division, National Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors: Long
authors: L
authors: Wu
authors: Yuan
authors: Y
authors: Li
authors: Mitsuhiro
authors: M
authors: Arakane
authors: Masakazu
authors: M
authors: Ike
authors: Masahisa
authors: M
authors: Wada
authors: Yoshifumi
authors: Y
authors: Terajima
authors: Shoko
authors: S
authors: Ishikawa
authors: Ken
authors: K
authors: Tokuyasu
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D031786:Saccharum / Q000187:drug effects*; D013696:Temperature; D014866:Waste Products / Q000032:analysis*
chemical_list: D000468:Alkalies; D014866:Waste Products; D000431:Ethanol; D002482:Cellulose; C027433:bagasse