title: The complete genome sequence of a member of a new species of tobamovirus (rattail cactus necrosis-associated virus) isolated from Aporcactus flagelliformis.
abstract: In this study, we identified a new tobamovirus from diseased Aporcactus flagelliformis cactus plants, named it rattail cactus necrosis-associated virus (RCNaV), and determined its complete genome sequence. The full RCNaV genome consisted of 6,506 nucleotides and contained four open reading frames coding for proteins of M(r) 128 kDa (3,441 nt), 185 kDa (4,929 nt), 55 kDa (1452 nt), 36 kDa (1,005 nt) and 19 kDa (513 nt) from the 5' to 3' end, respectively. The overall similarities for the four ORFs of RCNaV were from 32.5% to 64.1% and from 17.0% to 67.3% to those of the other tobamoviruses, at the nucleotide and amino acid level, respectively. Comparison of the coding and non-coding regions of the virus with those of other tobamoviruses showed that RCNaV is the most closely related to cactus mild mottle virus.
authors: Department of Horticulture, Biotechnology and Landscape Architecture, Plant Virus GenBank, Seoul Women's University, Seoul 139-774, Korea.
authors: N R
authors: NR
authors: Kim
authors: J S
authors: JS
authors: Hong
authors: Y S
authors: YS
authors: Song
authors: B N
authors: BN
authors: Chung
authors: J W
authors: JW
authors: Park
authors: Ki Hyun
authors: KH
authors: Ryu
mesh_terms: D001483:Base Sequence; D029421:Cactaceae / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017898:Tobamovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*