title: Isolation of 16 polymorphic microsatellite markers from an endangered and endemic species, Podocarpus nakaii (Podocarpaceae).
abstract: OBJECTIVE
  Sixteen polymorphic microsatellite markers were isolated and characterized from the endangered evergreen tree Podocarpus nakaii to evaluate the population structure for conservation efforts.
  
  
  RESULTS
  Based on a modified amplified fragment length polymorphism and magnetic bead enrichment method, 16 polymorphic primer sets were developed for this endangered insular species. Allele numbers ranged from five to seven, with an observed heterozygosity ranging from 0.29 to 0.88. Most primers were able to amplify DNA from the endemic P. fasciculus and the widely distributed P. macrophyllus var. macrophyllus, P. macrophyllus var. maki, and P. costalis.
  
  
  CONCLUSIONS
  The results reported here indicate the usefulness of codominant markers for future studies of the population genetics of P. nakaii. In addition, the markers are useful for further phytogeographic and speciation studies in P. fasciculus, P. macrophyllus var. macrophyllus, and P. macrophyllus var. maki, which are closely related species.
authors: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. yuchung@mail.nsysu.edu.tw
authors: Yu-Chung
authors: YC
authors: Chiang
authors: Huei-Chuan
authors: HC
authors: Shih
authors: Li-Wan
authors: LW
authors: Chang
authors: Wan-Rung
authors: WR
authors: Li
authors: Huan-Yu
authors: HY
authors: Lin
authors: Li-Ping
authors: LP
authors: Ju
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D056727:Endangered Species; D005819:Genetic Markers; D049810:Genetic Speciation; D005828:Genetics, Population; D005843:Geography; D006579:Heterozygote; D007564:Japan; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013624:Taiwan; D064028:Tracheophyta / Q000235:genetics*; D014197:Trees / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers