title: Characterization of the nuclear- and plastid-encoded secA-homologous genes in the unicellular red alga Cyanidioschyzon merolae.
abstract: SecA is an ATP-driven motor for protein translocation in bacteria and plants. Mycobacteria and listeria were recently found to possess two functionally distinct secA genes. In this study, we found that Cyanidioschyzon merolae, a unicellular red alga, possessed two distinct secA-homologous genes; one encoded in the cell nucleus and the other in the plastid genome. We found that the plastid-encoded SecA homolog showed significant ATPase activity at low temperature, and that the ATPase activity of the nuclear-encoded SecA homolog showed significant activity at high temperature. We propose that the two SecA homologs play different roles in protein translocation.
authors: Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
authors: Yosuke
authors: Y
authors: Koyama
authors: Koji
authors: K
authors: Takimoto
authors: Asuka
authors: A
authors: Kojima
authors: Kei
authors: K
authors: Asai
authors: Satoshi
authors: S
authors: Matsuoka
authors: Toshiaki
authors: T
authors: Mitsui
authors: Kouji
authors: K
authors: Matsumoto
authors: Hiroshi
authors: H
authors: Hara
authors: Niji
authors: N
authors: Ohta
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D020418:Algal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D018087:Plastids / Q000235:genetics*; D000461:Rhodophyta / Q000166:cytology* / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020418:Algal Proteins; D000251:Adenosine Triphosphatases