title: Protective effects of aqueous and ethanolic extracts of Portulaca oleracea L. aerial parts on H2O2-induced DNA damage in lymphocytes by comet assay.
abstract: The comet assay is a standard method for measuring DNA damage. In this study, the protective effects of ethanolic and aqueous extracts of Portulaca oleracea L. (P. oleracea) on human lymphocyte DNA lesions were evaluated with the comet assay. Lymphocytes were isolated from blood samples taken from healthy volunteers. Human lymphocytes were incubated in H(2)O(2) (50,100, and 200 μM), aqueous extract (0.05, 0.1, 0.5, 1, and 2.5mg/ml), and ethanolic extracts (0.05, 0.1, 0.5, 1, and 2.5mg/ml) of P. oleraceae aerial parts alone with a combination of H(2)O(2) (100 μM) with either 1 or 2.5mg/ml of both extracts at 4°C for 30 minutes. The extent of DNA migration was measured using the alkaline single cell gel electrophoresis approach assay, and DNA damage was expressed as percentage tail DNA. We found that the aqueous extract of P. oleracea significantly inhibited DNA damage, while there was no effect of the ethanolic extract. These data suggest that the aqueous extract of P. oleracea can prevent oxidative DNA damage to human lymphocytes, which is likely due to antioxidant constituents in the extract.
authors: Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Javad
authors: J
authors: Behravan
authors: Fatemeh
authors: F
authors: Mosafa
authors: Negar
authors: N
authors: Soudmand
authors: Elahe
authors: E
authors: Taghiabadi
authors: Bibi Marjan
authors: BM
authors: Razavi
authors: Gholamreza
authors: G
authors: Karimi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D020552:Comet Assay / Q000379:methods; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide; D008214:Lymphocytes / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D031822:Portulaca / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D004247:DNA; D006861:Hydrogen Peroxide