title: Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model.
abstract: Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities in a disease model. We anticipate that this approach will advance studies of synapse structure and function in brain development, plasticity, and disease.
authors: Department of Cell Biology, Emory University, 615 Michael St, NE, Atlanta, GA 30322, USA.
authors: Sharon A
authors: SA
authors: Swanger
authors: Xiaodi
authors: X
authors: Yao
authors: Christina
authors: C
authors: Gross
authors: Gary J
authors: GJ
authors: Bassell
mesh_terms: D000818:Animals; D001331:Automation; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology*; D049229:Dendritic Spines / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009025:Morpholines / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D013569:Synapses / Q000187:drug effects / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002867:Chromones; D051860:Fragile X Messenger Ribonucleoprotein 1; D009025:Morpholines; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D047428:Protein Kinase Inhibitors; C497684:Fmr1 protein, mouse; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one