title: Function of COP9 signalosome in regulation of mouse oocytes meiosis by regulating MPF activity and securing degradation.
abstract: The COP9 (constitutive photomorphogenic) signalosome (CSN), composed of eight subunits, is a highly conserved protein complex that regulates processes such as cell cycle progression and kinase signalling. Previously, we found the expression of the COP9 constitutive photomorphogenic homolog subunit 3 (CSN3) and subunit 5 (CSN5) changes as oocytes mature for the first time, and there is no report regarding roles of COP9 in the mammalian oocytes. Therefore, in the present study, we examined the effects of RNA interference (RNAi)-mediated transient knockdown of each subunit on the meiotic cell cycle in mice oocytes. Following knockdown of either CSN3 or CSN5, oocytes failed to complete meiosis I. These arrested oocytes exhibited a disrupted meiotic spindle and misarranged chromosomes. Moreover, down-regulation of each subunit disrupted the activity of maturation-promoting factor (MPF) and concurrently reduced degradation of the anaphase-promoting complex/cyclosome (APC/C) substrates Cyclin B1 and Securin. Our data suggest that the CSN3 and CSN5 are involved in oocyte meiosis by regulating degradation of Cyclin B1 and Securin via APC/C.
authors: Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea.
authors: Eunju
authors: E
authors: Kim
authors: Se-Jin
authors: SJ
authors: Yoon
authors: Eun-Young
authors: EY
authors: Kim
authors: Yunna
authors: Y
authors: Kim
authors: Hyun-Seo
authors: HS
authors: Lee
authors: Kyeoung-Hwa
authors: KH
authors: Kim
authors: Kyung-Ah
authors: KA
authors: Lee
mesh_terms: D000818:Animals; D000075686:COP9 Signalosome Complex; D002352:Carrier Proteins / Q000378:metabolism*; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation / Q000235:genetics; D033481:Chromosomes, Mammalian / Q000378:metabolism; D056744:Cyclin B1 / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008540:Meiosis; D000090204:Mesothelin; D051379:Mice; D008813:Mice, Inbred ICR; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D059748:Proteolysis; D011518:Proto-Oncogene Proteins; D034622:RNA Interference; D064249:Securin
chemical_list: D002352:Carrier Proteins; C000618791:Cops3 protein, mouse; D056744:Cyclin B1; D047908:Intracellular Signaling Peptides and Proteins; C000715528:Msln protein, mouse; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D064249:Securin; D011494:Protein Kinases; D016200:Maturation-Promoting Factor; D010447:Peptide Hydrolases; C501490:Cops5 protein, mouse; D000075686:COP9 Signalosome Complex; D000090204:Mesothelin