title: In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium.
abstract: In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of α-smooth muscle actin (α-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that α-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and α-SMA expression by these cells, especially in the fibronectin substratum.
authors: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil. efmartinez@ig.com.br
authors: Elizabeth F
authors: EF
authors: Martinez
authors: Ana Paula Dias
authors: AP
authors: Demasi
authors: Marcelo Henrique
authors: MH
authors: Napimoga
authors: Victor Elias
authors: VE
authors: Arana-Chavez
authors: Albina
authors: A
authors: Altemani
authors: Ney Soares
authors: NS
authors: de Araújo
authors: Vera Cavalcanti
authors: VC
authors: de Araújo
mesh_terms: D000199:Actins / Q000378:metabolism; D008949:Adenoma, Pleomorphic / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D024042:Collagen Type I / Q000494:pharmacology; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006801:Humans; D015676:Osteonectin / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D017077:Culture Media, Conditioned; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D015676:Osteonectin; D016222:Fibroblast Growth Factor 2