title: The new murine hepatic 3A cell line responds to stress stimuli by activating an efficient Unfolded Protein Response (UPR).
abstract: In the present study we have investigated the properties of a novel cell line (3A cells) obtained from the liver of 14.5 days post coitum (dpc) wild-type mouse embryo. 3A cells morphology was characterized by fluorescent localization of F-actin and β-catenin. The expression of specific genes and proteins essential to liver function in these cells was comparable or even more efficient then in the differentiated hepatocytic cell line MMH-D6. 3A cells also showed the capability to excrete molecules in extracellular spaces resembling functional bile canaliculi, glycogen storage activity and the ability to control retinol-binding protein 4 secretion in response to retinol deprivation. Their response to the exogenous stress stimulus induced by tunicamycin was analysed by PCR Pathway Array containing 84 genes involved in the Unfolded Protein Response (UPR). 3A cells were shown to activate the UPR following a typical stressful event, indicating that this cellular model could be further exploited to investigate hepatic proteins secretion and specific reaction to different injuries.
authors: National Research Institute on Food and Nutrition (INRAN), Rome, Italy.
authors: Barbara
authors: B
authors: Guantario
authors: Alice
authors: A
authors: Conigliaro
authors: Laura
authors: L
authors: Amicone
authors: Yula
authors: Y
authors: Sambuy
authors: Diana
authors: D
authors: Bellovino
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line / Q000378:metabolism*; D019793:Fluorescein / Q000378:metabolism; D020869:Gene Expression Profiling; D006003:Glycogen / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D054839:Retinol-Binding Proteins, Plasma / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological; D014415:Tunicamycin / Q000494:pharmacology*; D056811:Unfolded Protein Response / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D000199:Actins; C502487:Rbp4 protein, mouse; D054839:Retinol-Binding Proteins, Plasma; D051176:beta Catenin; D014415:Tunicamycin; D006003:Glycogen; D019793:Fluorescein