title: Bidirectional plasticity of calcium-permeable AMPA receptors in oligodendrocyte lineage cells.
abstract: Oligodendrocyte precursor cells (OPCs), a major glial cell type that gives rise to myelinating oligodendrocytes in the CNS, express calcium-permeable AMPA receptors (CP-AMPARs). Although CP-AMPARs are important for OPC proliferation and neuron-glia signaling, they render OPCs susceptible to ischemic damage in early development. We identified factors controlling the dynamic regulation of AMPAR subtypes in OPCs from rat optic nerve and mouse cerebellar cortex. We found that activation of group 1 mGluRs drove an increase in the proportion of CP-AMPARs, reflected by an increase in single-channel conductance and inward rectification. This plasticity required the elevation of intracellular calcium and used PI3K, PICK-1 and the JNK pathway. In white matter, neurons and astrocytes release both ATP and glutamate. Unexpectedly, activation of purinergic receptors in OPCs decreased CP-AMPAR expression, suggesting a capacity for homeostatic regulation. Finally, we found that stargazin-related transmembrane AMPAR regulatory proteins, which are critical for AMPAR surface expression in neurons, regulate CP-AMPAR plasticity in OPCs.
authors: Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.
authors: Marzieh
authors: M
authors: Zonouzi
authors: Massimiliano
authors: M
authors: Renzi
authors: Mark
authors: M
authors: Farrant
authors: Stuart G
authors: SG
authors: Cull-Candy
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000378:metabolism; D055592:Biophysical Phenomena / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D019070:Cell Lineage; D002531:Cerebellum / Q000166:cytology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018683:Excitatory Amino Acid Agents / Q000494:pharmacology; D005260:Female; D005698:Galactosylceramidase / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D018698:Glutamic Acid / Q000494:pharmacology; D018684:Glycine Agents / Q000494:pharmacology; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009473:Neuronal Plasticity / Q000235:genetics / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism; D013331:Strychnine / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D002118:Calcium; D015220:Calcium Channels; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D018683:Excitatory Amino Acid Agents; D005698:Galactosylceramidase; D018698:Glutamic Acid; D018684:Glycine Agents; D008164:Luminescent Proteins; D008734:Methoxyhydroxyphenylglycol; D009687:Nuclear Proteins; D011509:Proteoglycans; D018091:Receptors, AMPA; D013331:Strychnine; D013779:Tetrodotoxin; D000099216:Chondroitin Sulfate Proteoglycan 4; C113940:Cacng2 protein, mouse; C091822:Prkcabp protein, mouse; C413663:fluorescent protein 583; C010117:3,4-dihydroxyphenylglycol