title: Gossypin induces G2/M arrest in human malignant glioma U251 cells by the activation of Chk1/Cdc25C pathway.
abstract: Gossypin is a flavone that was originally isolated from Hibiscus                         vitifolius and has traditionally been used for the treatment of diabetes, jaundice, and inflammation. Recently, gossypin was found to have potent anticancer properties; however, its effect on human gliomas still remain unknown. To investigate the potential anticancer effects of gossypin on malignant gliomas and analyze the associated molecular mechanisms, we treated human glioma U251 cells with gossypin. Our study showed that the treatment of U251 cells with gossypin inhibited cell proliferation in a dose- and time-dependent manner and was observed to be minimally toxic to normal human astrocytes. Gossypin's effect on cell cycle distribution was observed, and we found that it induced G2/M-phase arrest in U251 cells. An analysis of cell-cycle regulatory proteins indicated that the arresting effect of gossypin on the cell cycle at G2/M phase was involved in the phosphorylation of cell division cycle 25C (Cdc25C) tyrosine phosphatase via the activation of checkpoint kinase 1 (Chk1). These findings indicate that gossypin is a potential treatment of gliomas because of gossypin's potential to regulate the proliferation of U251 cells via the cell-cycle regulatory proteins Chk1 and Cdc25C.
authors: Department of Neurosurgery, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215300, People's Republic of China. shileigff@126.com
authors: Lei
authors: L
authors: Shi
authors: Jian
authors: J
authors: Chen
authors: Yin-yi
authors: YY
authors: Wang
authors: Guan
authors: G
authors: Sun
authors: Jing-ning
authors: JN
authors: Liu
authors: Jun-xia
authors: JX
authors: Zhang
authors: Wei
authors: W
authors: Yan
authors: Chun-fa
authors: CF
authors: Qian
authors: Ning
authors: N
authors: Liu
authors: Zhen
authors: Z
authors: Fu
authors: Yong-ping
authors: YP
authors: You
authors: Yanjun
authors: Y
authors: Zeng
mesh_terms: D001253:Astrocytes / Q000187:drug effects / Q000473:pathology; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000071877:Checkpoint Kinase 1; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D005910:Glioma / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D020687:cdc25 Phosphatases / Q000378:metabolism*
chemical_list: D005419:Flavonoids; C022944:gossypin; D011494:Protein Kinases; C000605867:CHEK1 protein, human; D000071877:Checkpoint Kinase 1; D020687:cdc25 Phosphatases