title: MiR-29a inhibits cell proliferation and induces cell cycle arrest through the downregulation of p42.3 in human gastric cancer.
abstract: As a newly identified and characterized gene, p42.3 is associated with cell proliferation and tumorigenicity. The expression of p42.3 is upregulated in human gastric cancer (GC), but its underlying mechanisms of action are not well understood. MicroRNAs (miRNAs) are known to play vital regulatory roles in many cellular processes. Here we utilized bioinformatics and experimental approaches to investigate the regulatory relationship between miRNAs and the p42.3 gene. We showed that miR-29a could repress p42.3 expression at both the mRNA and protein levels via directly binding to its 3'UTR. Furthermore, an inverse relationship was observed between miR-29a and p42.3 expression in gastric cancer cell lines and GC tissue samples, especially in cases where p42.3 was downregulated. Taken together, we have elucidated previously unrecognized roles of miR-29a and indicated that miR-29a may function, at least partially, by targeting the p42.3 gene in human GC.
authors: Division of Gastroenterology and Hepatology, Shanghai Jiao-Tong University School of Medicine Renji Hospital, Shanghai Institute of Digestive Disease, Shanghai, China.
authors: Yun
authors: Y
authors: Cui
authors: Wen-Yu
authors: WY
authors: Su
authors: Jing
authors: J
authors: Xing
authors: Ying-Chao
authors: YC
authors: Wang
authors: Ping
authors: P
authors: Wang
authors: Xiao-Yu
authors: XY
authors: Chen
authors: Zhi-Yong
authors: ZY
authors: Shen
authors: Hui
authors: H
authors: Cao
authors: You-Yong
authors: YY
authors: Lu
authors: Jing-Yuan
authors: JY
authors: Fang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000368:Aged; D001483:Base Sequence; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009687:Nuclear Proteins; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D009687:Nuclear Proteins; C000607889:SAPCD2 protein, human