title: Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy.
abstract: The visualization and quantification of mitochondria-associated proteins with high power microscopy methods is of particular interest to investigate protein architecture in this organelle. We report the usage of a custom-made STimulated Emission Depletion (STED) fluorescence nanoscope with ~30nm lateral resolution for protein mapping of Percoll-purified viable mitochondria from murine heart. Using this approach, we were able to quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c oxidase subunit 2 is distributed in clusters of ~28nm; whereas the voltage dependent anion channel 1 displays three size distributions of ~33, ~55 and ~83nm.
authors: Department of Anesthesiology, Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors: Harpreet
authors: H
authors: Singh
authors: Rong
authors: R
authors: Lu
authors: Pedro Felipe Gardeazábal
authors: PF
authors: Rodríguez
authors: Yong
authors: Y
authors: Wu
authors: Jean Chrisostome
authors: JC
authors: Bopassa
authors: Enrico
authors: E
authors: Stefani
authors: Ligia
authors: L
authors: Toro
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry*
chemical_list: D024101:Mitochondrial Proteins