title: A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties.
abstract: Pyrolytic graphite particles modified with hydrogenase and an NAD(+)/NADH cycling enzyme provide a modular heterogeneous catalyst system for regeneration of oxidised or reduced nicotinamide cofactors using H(2) and H(+) as electron source or sink. Particles can be tuned for cofactor supply under different conditions by appropriate choice of hydrogenase.
authors: Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.
authors: Holly A
authors: HA
authors: Reeve
authors: Lars
authors: L
authors: Lauterbach
authors: Philip A
authors: PA
authors: Ash
authors: Oliver
authors: O
authors: Lenz
authors: Kylie A
authors: KA
authors: Vincent
mesh_terms: D055162:Biocatalysis; D006108:Graphite / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000737:chemistry / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010316:Particle Size
chemical_list: D009243:NAD; D006108:Graphite; D006864:Hydrogenase; D009252:NADPH Dehydrogenase