title: Enhanced Schwann cell adhesion and elongation on a topographically and chemically modified poly(L-lactic acid) film surface.
abstract: A dense poly-L-lactic acid (PLLA) film was employed as the primary material and hot-embossed with the formation of microgrooves (g-PLLA). A thin layer of Au was then deposited on the film to obtain a morphologically modified substrate (Au/g-PLLA). The Au/g-PLLA film surface was then chemically modified by imprinting octadecanethiolate (ODT) self-assembled monolayers on the upper surface (ODT/Au/g-PLLA), followed by Arg-Gly-Asp (RGD) peptide sequences on the microgrooves (RGD_ODT/Au/g-PLLA). The surface chemistry of the as-prepared RGD_ODT/Au/g-PLLA samples was examined. The bioactivity and spreading function of Schwann cells cultured on the morphologically and chemically modified surfaces were assessed. The results demonstrate that Schwann cells adhered to the RGD/Au/g-PLLA surface and proliferated along the microgrooves without crossing over the ODT/Au/PLLA surface. The proposed film surface can be used for manipulating the outgrowth of axons by modifying and arranging a selected region to induce cell growth and to prevent cells from spreading out nondirectionally.
authors: Department of Materials Science and Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors: Wei-Chin
authors: WC
authors: Huang
authors: Chih-Kai
authors: CK
authors: Yao
authors: Jiunn-Der
authors: JD
authors: Liao
authors: Chou-Ching K
authors: CC
authors: Lin
authors: Ming-Shaung
authors: MS
authors: Ju
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D006046:Gold; C047981:arginyl-glycyl-aspartic acid