title: Tissue-Specific stem cell differentiation in an in vitro airway model.
abstract: The respiratory tract is the primary site of exposure to airborne compounds, with the bronchial epithelium providing one of the first lines of defence. A growing need exists for an accurate in vitro model of the bronchial epithelium. Here, normal human bronchial epithelial (NHBE) cells cultured at an air/liquid interface create a fully differentiated, in-vivo-like model of the human bronchial epithelium. Developmental characterisation includes (i) trans-epithelial electrical resistance, (ii) morphology and (iii) bronchial cell specific stains/markers. It is concluded that the basal/progenitor cells create a pseudo-stratified, mucociliary NHBE model containing basal, serous, Clara, goblet and ciliated cells, reflective of the normal human bronchial epithelium (days 24-33 ALI culture).
authors: School of Biosciences, Cardiff University, Cardiff, UK. prytherchzc@cf.ac.uk
authors: Zoë
authors: Z
authors: Prytherch
authors: Claire
authors: C
authors: Job
authors: Hilary
authors: H
authors: Marshall
authors: Victor
authors: V
authors: Oreffo
authors: Martyn
authors: M
authors: Foster
authors: Kelly
authors: K
authors: BéruBé
mesh_terms: D001344:Autopsy; D015415:Biomarkers / Q000032:analysis; D001980:Bronchi / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D017097:Electric Impedance; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D020545:Respiratory Mucosa / Q000166:cytology* / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D015415:Biomarkers