title: Intensity of cytosol expression of 8-OHdG in normal renal tubules is associated with the severity of renal fibrosis.
abstract: BACKGROUND
  It has been proposed that reactive oxygen species play a role in renal fibrosis. 8-OHdG, a metabolite of oxidative damage to leukocyte DNA, has been identified as a marker of oxidative stress in patients with chronic renal failure.
  
  
  METHODS
  Seventy-four patients following nephrectomy were retrospectively enrolled. Immunohistochemical analysis of the renal expression of 8-OHdG in the nephrectomised kidneys was performed and associations between renal expression of 8-OHdG and renal fibrosis were evaluated.
  
  
  RESULTS
  Patients with higher interstitial fibrosis scores (IFS) and glomerular fibrosis scores (GFS) had significantly higher serum creatinine, lower estimated glomerular filtration rate (eGFR), increased percentage of chronic kidney disease (CKD) and urothelial cell carcinoma. The renal tissues with higher IFS had lower expressions of 8-OHdG in normal tubular cytoplasm (NTc) (35.7% vs. 64.3%, p = 0.011) and normal tubular nuclei (NTn) (28.6% vs. 71.4%, p = 0.023). Univariate analysis showed that IFS and GFS correlated with the NTc 8-OHdG expression and IFS negatively correlated with NTn 8-OHdG expression. Multivariate stepwise regression revealed that serum creatinine (r = 0.351 for IFS, p = 0.021; r = 0.563 for GFS, p < 0.001) and intensity of 8-OHdG expression in NTc (r = 0.397 for IFS, p = 0.01; r = 0.278 for GFS, p = 0.043) were the independent factors predicting IFS or GFS.
  
  
  CONCLUSIONS
  This study demonstrated that the intensity of 8-OHdG expression in NTc was associated with the severity of renal fibrosis.
authors: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors: Jen-Pi
authors: JP
authors: Tsai
authors: Jia-Hung
authors: JH
authors: Liou
authors: Kun-Tu
authors: KT
authors: Yeh
authors: Hui-Chun
authors: HC
authors: Tai
authors: Ya-Wen
authors: YW
authors: Cheng
authors: Horng-Rong
authors: HR
authors: Chang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005355:Fibrosis / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007676:Kidney Failure, Chronic / Q000503:physiopathology*; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine