title: Expression of Kisspeptin and its receptor GPR54 in the first trimester trophoblast of women with recurrent pregnancy loss.
abstract: OBJECTIVE
  Kisspeptin and its receptor GPR54 play a major role in trophoblast invasion. The expression of kisspeptin and GPR54 in trophoblast and decidua and their relationship with decidual and peripheral blood natural killer (NK) cells are investigated in women with RPL.
  
  
  METHODS
  Trophoblast and decidual tissues were collected from 38 RPL women who miscarried a genetically normal fetus and 14 women who had elective abortion. Kisspeptin, GPR54, and decidual NK cells were investigated with immunohistochemistry, and peripheral blood NK cells were analyzed by flow cytometry.
  
  
  RESULTS
  Kisspeptin expression in syncytiotrophoblast was significantly decreased in RPL women with normal (<15%) peripheral blood NK cells (npNK) (P=0.021) and high (≥15%) peripheral blood NK cells (hpNK) (P=0.024) as compared to controls. Kisspeptin expression in cytotrophoblast was significantly decreased hpNK group (P=0.009) as compared to controls. GPR54 expressions were not different among study groups and controls. The number of CD56(+) decidual NK cells are significantly higher in hpNK group as compared to npNK group (P=0.041) and showed a correlation with kisspeptin expression in syncytiotrophoblasts (r=0.738, P<0.001).
  
  
  CONCLUSIONS
  Decreased kisspeptin expression in trophoblasts is associated with RPL and kisspeptin may engage the regulation of decidual NK cell infiltration.
authors: Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University, College of Medicine, Seoul, Korea.
authors: Dong-Wook
authors: DW
authors: Park
authors: Sung-Ki
authors: SK
authors: Lee
authors: Sung Ran
authors: SR
authors: Hong
authors: Ae-Ra
authors: AR
authors: Han
authors: Joanne
authors: J
authors: Kwak-Kim
authors: Kwang Moon
authors: KM
authors: Yang
mesh_terms: D000026:Abortion, Habitual / Q000276:immunology / Q000378:metabolism*; D000028:Abortion, Induced; D019002:CD56 Antigen / Q000378:metabolism; D003656:Decidua / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D059648:Kisspeptins / Q000378:metabolism*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology / Q000378:metabolism; D011261:Pregnancy Trimester, First; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D000074502:Receptors, Kisspeptin-1; D014327:Trophoblasts / Q000276:immunology / Q000378:metabolism*
chemical_list: D019002:CD56 Antigen; C106628:KISS1 protein, human; C118688:KISS1R protein, human; D059648:Kisspeptins; D043562:Receptors, G-Protein-Coupled; D017452:Receptors, IgG; D000074502:Receptors, Kisspeptin-1