title: Deciphering the cellular and molecular roles of cellular nucleic acid binding protein during cranial neural crest development.
abstract: Cellular nucleic acid binding protein (Cnbp) is a highly conserved single-stranded nucleic acid binding protein required for rostral head development. The use of a morpholino that inhibits Cnbp mRNA translation previously revealed a role of Cnbp in balancing neural crest cell apoptosis and proliferation in the developing zebrafish. Here, we report the use of another morpholino that specifically modifies the splicing of Cnbp pre-mRNA resulting in a reduction of full-length mRNA levels along with the generation of a novel transcript coding for an isoform that may act as dominant negative proteins. The use of this morpholino resulted in more severe phenotypes that enabled us to demonstrate that Cnbp loss-of-function adversely affects the formation and survival of craniofacial cartilaginous structures not only controlling the ratio of cell proliferation and apoptosis but also defining skeletogenic neural crest cell fate.
authors: Molecular and Cellular Biology Institute (IBR), National Council of Scientific and Technological Research (CONICET)-Biology Area, Department of Biological Sciences, Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 531, Rosario, S2002LRK, Argentina.
authors: Andrea M J
authors: AM
authors: Weiner
authors: María A
authors: MA
authors: Sdrigotti
authors: Robert N
authors: RN
authors: Kelsh
authors: Nora B
authors: NB
authors: Calcaterra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D055785:Gene Knockdown Techniques; D008954:Models, Biological; D008969:Molecular Sequence Data; D060172:Morpholinos / Q000494:pharmacology; D009432:Neural Crest / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017385:Sequence Homology; D012886:Skull / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D060172:Morpholinos; D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C499019:cnbpa protein, zebrafish