title: Differentiating ovine BSE from CH1641 scrapie by serial protein misfolding cyclic amplification.
abstract: Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V₁₃₆R₁₅₄Q₁₇₁/V₁₃₆R₁₅₄Q₁₇₁ or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.
authors: School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire LE12 5RD, UK.
authors: Maged M
authors: MM
authors: Taema
authors: Ben C
authors: BC
authors: Maddison
authors: Leigh
authors: L
authors: Thorne
authors: Keith
authors: K
authors: Bishop
authors: Jonathan
authors: J
authors: Owen
authors: Nora
authors: N
authors: Hunter
authors: Claire A
authors: CA
authors: Baker
authors: Linda A
authors: LA
authors: Terry
authors: Kevin C
authors: KC
authors: Gough
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D003937:Diagnosis, Differential; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000378:metabolism; D005838:Genotype; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D011328:Prions / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D012608:Scrapie / Q000175:diagnosis* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods; D034561:Sheep, Domestic
chemical_list: D011328:Prions