title: Possible role of the JAK/STAT pathways in the regulation of T cell-interferon related genes in systemic lupus erythematosus.
abstract: Changes in gene expression in CD3+ T cells associated with disease progression in systemic lupus erythematosus (SLE) patients were determined. The genes related to SLE disease-related activities were identified and their gene regulatory networks were investigated. Analyses of gene expression were performed by both DNA microarray and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression of certain genes including interferon (IFN) regulatory factor (IRF)-related genes, such as IFN-regulated, -related, and -signature genes was increased in the active phase of SLE. Pathway network analyses suggested that these IRF-related genes are regulated through the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway. JAK/STAT pathway-mediated regulation of IRF-related genes may have an important role in the disease activity of SLE. Inhibitors of JAK/STAT cascade may be useful as therapeutic agents.
authors: Institute for Environmental and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan.
authors: M
authors: M
authors: Kawasaki
authors: M
authors: M
authors: Fujishiro
authors: A
authors: A
authors: Yamaguchi
authors: K
authors: K
authors: Nozawa
authors: H
authors: H
authors: Kaneko
authors: Y
authors: Y
authors: Takasaki
authors: K
authors: K
authors: Takamori
authors: H
authors: H
authors: Ogawa
authors: I
authors: I
authors: Sekigawa
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics*; D053612:Janus Kinases / Q000235:genetics* / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050791:STAT Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
chemical_list: D017931:DNA Primers; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger; D050791:STAT Transcription Factors; D053612:Janus Kinases