title: [Construction of vectors expressing M2 and NA genes of H5N1 influenza virus].
abstract: OBJECTIVE
  To construct vectors expressing M2 and NA genes of H5N1 influenza virus.
  
  
  METHODS
  Based on the human H5N1 avian influenza virus (A/Anhui/1/2005) isolated in china, M2 and NA genes were amplified by PCR. M2 or NA gene was subcloned into pStar vector to construct recombinant pStar-M2/, pStar-/M2, pStar-NA/and pStar-NA/. Furthermore, both of the M2 and NA genes were subcloned into pStar to construct two genes co-expressing recombinant pStar-M2/NA and pStar-NA/M2. Expression of the genes were detected by IFA after transfection of 293 cells with the recombinant plasmids.
  
  
  RESULTS
  Recombinant plasmids were constructed and identified by restriction endonuclease digestion. Expression of the genes cloned into the recombinant plasmids was confirmed by IFA.
  
  
  CONCLUSIONS
  Recombinant plasmids expressing M2 and/or NA genes of H5N1 influenza virus were constructed, which provided basis for development of influenza DNA vaccine.
authors: State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors: Jian-Qiang
authors: JQ
authors: Guo
authors: Li-Hong
authors: LH
authors: Yao
authors: Ai-Jun
authors: AJ
authors: Chen
authors: Xiao-Yu
authors: XY
authors: Liu
authors: Jin-Qi
authors: JQ
authors: Fu
authors: Peng-Wei
authors: PW
authors: Xu
authors: Zhi-Qing
authors: ZQ
authors: Zhang
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000378:metabolism*; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D009439:Neuraminidase; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins; C487630:NA protein, influenza A virus