title: Parasite limiting macrophages promote IL-17 secretion in naive bovine CD4⁺ T-cells during Neospora caninum infection.
abstract: Neospora caninum infects bovine hosts giving rise to pro-inflammatory immune responses that can result in foetal death or spontaneous abortion, this appears to be mediated by the actions of IFN-γ on cell activation and migration/trafficking. Yet successful vaccination or natural immunity is also strongly correlated with IFN-γ production. We utilised in vitro infection of bovine macrophages to prime naive T-cell responses. Naive T-cells in contact with infected macrophages produce both IFN-γ and IL-17 in a pattern that is dependent on whether the priming macrophage was protected or non-protected. Our results may explain the apparent dual role of IFN-γ during infection if a second major pro-inflammatory cytokine, IL-17, is produced simultaneously.
authors: School of Veterinary Medicine & Science, University of Nottingham, United Kingdom. robin.flynn@nottingham.ac.uk
authors: Robin J
authors: RJ
authors: Flynn
authors: Edward S
authors: ES
authors: Marshall
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D002478:Cells, Cultured; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D016207:Cytokines / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D020381:Interleukin-17 / Q000378:metabolism*; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000502:physiology; D018688:Neospora / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D015850:Interleukin-6; D007371:Interferon-gamma