title: Lymphocyte subtypes CD3+, CD19+, CD16+CD56+, CD4+, CD8+, and CD3+HLA-DR+ in peripheral blood obtained from patients after thoracic organ transplantation.
abstract: BACKGROUND
  The aim of this study was to assess peripheral blood lymphocyte subtypes (CD3+, CD19+, CD16+CD56+, CD4+, CD8+, and CD3+HLA-DR+) obtained from thoracic organ recipients at various periods after transplantation.
  
  
  METHODS
  Seventeen patients after lung transplantation (LT) and 5 patients after heart transplantation (HT) included 13 males (76.5%) and 4 females (23.5%) of overall mean age at the time of transplantation of 46.7±11.55 years and mean body mass index of 21.1±4. Lymphocyte phenotypes were estimated using Simultest IMK Plus.
  
  
  RESULTS
  A significant decrease in lymphocytes of the majority of subtypes was observed at 1 year posttransplantation compared with normal ranges: CD19+ B lymphocytes in 56% of patients, CD8+ T cells among 48% and CD16+CD56+ natural killer elements, 56%. In contrast, there were increased numbers of activated lymphocytes (CD3+HLA-DR+). Beyond the 1-year observation, we observed a trend to normalize parameters among the majority of subjects.
  
  
  CONCLUSIONS
  A clear tendency to a decrease number of peripheral blood lymphocytes of various subtypes was observed among thoracic organ recipients in the first year posttransplantation with the exception of activated HLA-DR+ cells. After the first year, there was slow restoration of lymphocytes.
authors: Department and Clinic of Cardiac Surgery and Transplantology, Silesian Centre for Heart Diseases, Zabrze, Poland. slawekzeglen@poczta.onet.pl
authors: S
authors: S
authors: Zegleń
authors: A
authors: A
authors: Łaszewska
authors: J
authors: J
authors: Wojarski
authors: E
authors: E
authors: Woźniak-Grygiel
authors: M
authors: M
authors: Zakliczyński
authors: M
authors: M
authors: Ochman
authors: P
authors: P
authors: Wilczek
authors: W
authors: W
authors: Karolak
authors: J
authors: J
authors: Nożyński
authors: M
authors: M
authors: Zembala
mesh_terms: D000328:Adult; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000276:immunology; D006684:HLA-DR Antigens / Q000097:blood; D016027:Heart Transplantation / Q000009:adverse effects* / Q000276:immunology*; D006801:Humans; D016040:Lung Transplantation / Q000009:adverse effects* / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008231:Lymphopenia / Q000209:etiology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000097:blood / Q000276:immunology; D013997:Time Factors; D014181:Transplantation Immunology
chemical_list: D006684:HLA-DR Antigens