title: Identification of distinct lipopolysaccharide patterns among Yersinia enterocolitica and Y. enterocolitica-like bacteria.
abstract: The lipopolysaccharide (LPS) of strains representing various serotypes of Yersinia enterocolitica and Y. enterocolitica-like bacteria was studied by deoxycholate-PAGE and silver staining analysis. Four main types of LPS were detected based on the O-polysaccharide (O-PS): (i) LPS with homopolymeric O-PS, (ii) LPS with ladder-forming heteropolymeric O-PS, (iii) LPS with single-length O-PS, and (iv) semi-rough LPS without O-PS. Within the first three types, several subvariants were detected. Selected serotypes representing all above LPS types are sensitive to bacteriophage φR1-37 indicating that they share the phage receptor, a hexasaccharide called outer core in Y. enterocolitica O:3. Whereas phage φR1-37-resistant mutants of homopolymeric O-PS have lost only the outer core, those of ladder-forming or single-length O-PS have lost also the O-PS suggesting that in the latter ones the outer core is bridging between O-PS and lipid A-core. This work forms a basis of further structural, biochemical and genetic studies of these LPSs.
authors: Department of Bacteriology and Immunology, Haartman Institute, Infection Biology Research Program, University of Helsinki, Finland. mikael.skurnik@helsinki.fi
authors: M
authors: M
authors: Skurnik
authors: S
authors: S
authors: Toivonen
mesh_terms: D001435:Bacteriophages / Q000472:pathogenicity; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D019081:O Antigens / Q000737:chemistry; D015007:Yersinia / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D015008:Yersinia enterocolitica / Q000235:genetics / Q000276:immunology* / Q000821:virology
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens