title: The role of hepatic liver X receptor α- and sterol regulatory element binding protein-1c-mediated lipid disorder in the pathogenesis of non-alcoholic steatohepatitis in rats.
abstract: Liver X receptor α (LXRα) and sterol regulatory element binding protein-1c (SREBP-1c) were studied in rats with non-alcoholic steatohepatitis (NASH) induced by a high-fat diet. Forty 5-week-old rats were fed either a high-fat diet (n = 30) or a normal diet (n  = 10) for 9, 13 or 17 weeks. The mRNA and protein levels for LXRα and SREBP-1c were measured at each time point, as was fatty acid synthase (FAS) activity and the serum levels of free fatty acid (FFA) and triglyceride (TG). The mRNA and protein levels for LXRα and SREBP-1c, FAS  activity and serum levels of FFA and TG all significantly increased from week 9 in the high-fat diet rats versus controls. In conclusion, a high-fat diet upregulates LXRα which, in turn, upregulates SREBP-1c, increasing the activity of FAS and FFA and accumulation of TG in hepatocytes.  Thus, LXRα and SREBP-1c contribute to the development of NASH.
authors: Department of Gastroenterology and Hepatology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
authors: Z-L
authors: ZL
authors: Ai
authors: C-H
authors: CH
authors: Zhu
authors: M
authors: M
authors: Min
authors: J
authors: J
authors: Wang
authors: C-H
authors: CH
authors: Lan
authors: L-L
authors: LL
authors: Fan
authors: W-J
authors: WJ
authors: Sun
authors: D-F
authors: DF
authors: Chen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D059305:Diet, High-Fat; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005234:Fatty Liver / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*; D019014:fas Receptor / Q000097:blood
chemical_list: C491898:Fas protein, rat; D005230:Fatty Acids, Nonesterified; D000071518:Liver X Receptors; C000605215:Nr1h3 protein, rat; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D019014:fas Receptor