title: Proteomics and pluripotency.
abstract: The fields of mass spectrometry (MS) and stem cell biology have expanded greatly in the past twenty years. Taken alone, these fields occupy entirely different branches of science; however, the points where they overlap provide valuable insight, both in the biological and technical arenas. From a biological perspective, MS-based proteomics offers the capacity to follow post-transcriptional regulation and signaling that are (1) fundamental to pluripotency and differentiation, (2) largely beyond the reach of genomic technologies, and (3) otherwise difficult or impossible to examine on a large scale. At the same time, addressing questions fundamental to stem cell biology has compelled proteomic researchers to pursue more sensitive and creative ways to probe the proteome, both in a targeted and high-throughput manner. Here, we highlight experiments that straddle proteomics and stem cell biology, with an emphasis on studies that apply mass spectrometry to dissect pluripotency and differentiation.
authors: Department of Biomolecular Chemistry, University of Wisconsin-Madison, USA.
authors: Justin
authors: J
authors: Brumbaugh
authors: Christopher M
authors: CM
authors: Rose
authors: Douglas H
authors: DH
authors: Phanstiel
authors: James A
authors: JA
authors: Thomson
authors: Joshua J
authors: JJ
authors: Coon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D011506:Proteins; D020543:Proteome