title: Toward an understanding of the protein interaction network of the human liver.
abstract: Proteome-scale protein interaction maps are available for many organisms, ranging from bacteria, yeast, worms and flies to humans. These maps provide substantial new insights into systems biology, disease research and drug discovery. However, only a small fraction of the total number of human protein-protein interactions has been identified. In this study, we map the interactions of an unbiased selection of 5026 human liver expression proteins by yeast two-hybrid technology and establish a human liver protein interaction network (HLPN) composed of 3484 interactions among 2582 proteins. The data set has a validation rate of over 72% as determined by three independent biochemical or cellular assays. The network includes metabolic enzymes and liver-specific, liver-phenotype and liver-disease proteins that are individually critical for the maintenance of liver functions. The liver enriched proteins had significantly different topological properties and increased our understanding of the functional relationships among proteins in a liver-specific manner. Our data represent the first comprehensive description of a HLPN, which could be a valuable tool for understanding the functioning of the protein interaction network of the human liver.
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.
authors: Jian
authors: J
authors: Wang
authors: Keke
authors: K
authors: Huo
authors: Lixin
authors: L
authors: Ma
authors: Liujun
authors: L
authors: Tang
authors: Dong
authors: D
authors: Li
authors: Xiaobi
authors: X
authors: Huang
authors: Yanzhi
authors: Y
authors: Yuan
authors: Chunhua
authors: C
authors: Li
authors: Wei
authors: W
authors: Wang
authors: Wei
authors: W
authors: Guan
authors: Hui
authors: H
authors: Chen
authors: Chaozhi
authors: C
authors: Jin
authors: Juncheng
authors: J
authors: Wei
authors: Wanqiao
authors: W
authors: Zhang
authors: Yongsheng
authors: Y
authors: Yang
authors: Qiongming
authors: Q
authors: Liu
authors: Ying
authors: Y
authors: Zhou
authors: Cuili
authors: C
authors: Zhang
authors: Zhihao
authors: Z
authors: Wu
authors: Wangxiang
authors: W
authors: Xu
authors: Ying
authors: Y
authors: Zhang
authors: Tao
authors: T
authors: Liu
authors: Donghui
authors: D
authors: Yu
authors: Yaping
authors: Y
authors: Zhang
authors: Liang
authors: L
authors: Chen
authors: Dewu
authors: D
authors: Zhu
authors: Xing
authors: X
authors: Zhong
authors: Lixin
authors: L
authors: Kang
authors: Xiang
authors: X
authors: Gan
authors: Xiaolan
authors: X
authors: Yu
authors: Qi
authors: Q
authors: Ma
authors: Jing
authors: J
authors: Yan
authors: Li
authors: L
authors: Zhou
authors: Zhongyang
authors: Z
authors: Liu
authors: Yunping
authors: Y
authors: Zhu
authors: Tao
authors: T
authors: Zhou
authors: Fuchu
authors: F
authors: He
authors: Xiaoming
authors: X
authors: Yang
mesh_terms: D030562:Databases, Protein; D020868:Gene Silencing / Q000187:drug effects; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000032:analysis; D016366:Open Reading Frames; D010957:Plasmids; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D034741:RNA, Small Interfering / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D049490:Systems Biology; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins; D020543:Proteome; D034741:RNA, Small Interfering; D008156:Luciferases