title: Impact of diet deprivation and subsequent over-allowance during prepuberty. Part 1. Effects on growth performance, metabolite status, and mammary gland development in gilts.
abstract: The impact of diet deprivation and subsequent over-allowance on the metabolite status, mammary development, and mammary gene expression in prepubertal gilts was determined. Forty-seven gilts were reared under a conventional (control, CTL; n = 23) or an experimental (treatment, TRT; n = 24) dietary regimen. The later regimen (consisting of diet deprivation and subsequent over-allowance) provided 70 (restriction diet, RES) and 115% (over-allowance diet, OVER) of the protein and DE contents provided by the CTL diet. Experimental diets were fed ad libitum starting at 27.7 ± 3.4 kg of BW as follows: 3 wk RES, 3 wk OVER, 4 wk RES, and 4 wk OVER. At each diet change, BW and individual feed intakes were measured, and blood samples for metabolite and IGF-I assays were obtained. Some gilts (11 CTL and 12 TRT) were slaughtered on d 235 (after reaching puberty) to collect mammary tissue for compositional analyses and measures of gene expression. Body weight gain (P < 0.01) and G:F (P < 0.05) of gilts were reduced during each period with the RES diet; however, there was no compensatory growth during the periods when the OVER diet was fed. Feeding the RES diet reduced concentrations of urea and IGF-I (P < 0.01) and feeding the OVER diet increased FFA (P < 0.01) and glucose (P < 0.10) in TRT gilts compared with CTL gilts. The TRT gilts had less parenchymal tissue (P < 0.05) and tended to have less total parenchymal fat and protein (P < 0.10) than CTL gilts. The mammary mRNA relative abundance of the signal transducers and activators of transduction 5B was decreased in TRT compared with CTL gilts (P < 0.05). In conclusion, the diet deprivation and over-allowance regimen used in the growing-finishing period did not have beneficial effects on mammary development after puberty. In fact, a detrimental effect was observed.
authors: Agriculture and Agri-Food Canada, Dairy and Swine R & D Centre, 2000 College St., Sherbrooke, Québec J1M 0C8, Canada. chantal.farmer@agr.gc.ca
authors: C
authors: C
authors: Farmer
authors: M F
authors: MF
authors: Palin
authors: Y
authors: Y
authors: Martel-Kennes
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose; D018076:DNA, Complementary / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005508:Food Deprivation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D007334:Insulin-Like Growth Factor I; D008321:Mammary Glands, Animal / Q000254:growth & development*; D010053:Ovary / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012741:Sexual Maturation / Q000187:drug effects; D013552:Swine / Q000097:blood / Q000254:growth & development* / Q000502:physiology*; D014508:Urea / Q000097:blood
chemical_list: D001786:Blood Glucose; D018076:DNA, Complementary; D005230:Fatty Acids, Nonesterified; D012313:RNA; D007334:Insulin-Like Growth Factor I; D014508:Urea