title: South American camelid illegal traffic detection by means of molecular markers.
abstract: South American camelids comprise the wild species guanaco and vicuña and their respective domestic relatives llama and alpaca. The aim of the present study was to determine by DNA analysis to which of these species belong a herd of camelids confiscated from a llama breeder but alleged to be alpacas by the prosecution, and to evaluate the usefulness of mitochondrial and autosomal DNA markers to solve judicial cases involving camelid taxa. Cytochrome b and cytochrome oxidase I mitochondrial genes and 7 STR were analyzed in 25 confiscated samples. Mitochondrial results were inconclusive because 18 of the sequestered samples presented haplotypes that corresponded to the guanaco haplogroup and the remaining seven belonged to a vicuña linage. Microsatellite data of casework samples and llama reference samples revealed different genetic profiles by the presence of private alleles at two microsatellites suggesting that the confiscated animals could be alpaca, or at least alpaca hybrids instead of pure llama.
authors: Instituto Multidisciplinario de Biología Celular, CCT-CONICET, La Plata, Argentina.
authors: F
authors: F
authors: Di Rocco
authors: D M
authors: DM
authors: Posik
authors: M V
authors: MV
authors: Ripoli
authors: S
authors: S
authors: Díaz
authors: M L
authors: ML
authors: Maté
authors: G
authors: G
authors: Giovambattista
authors: L
authors: L
authors: Vidal-Rioja
mesh_terms: D000818:Animals; D001118:Argentina; D002161:Camelids, New World / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000097:blood; D030541:Databases, Genetic; D005607:Fraud / Q000331:legislation & jurisprudence; D005819:Genetic Markers; D060005:Genotyping Techniques; D013020:South America; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers