title: Comparison of P19-derived neuroprogenitor and naive cell survival after intracerebellar application into B6CBA mice.
abstract: Mouse embryonic carcinoma cells (P19             line) were studied for both their survival and developmental             potential in the intact cerebellum of B6CBA             mice. The P19 cells were cultured and labelled with             green fluorescent protein using transfection. Cells             were used for transplantation either in the undifferentiated             stage or after 3 days of neurodifferentiation             induced by retinoic acid. The intracerebellar application             was performed in 43 mice: group A (N = 21)             received neuroprogenitors and group B (N = 22) received             undifferentiated cells. The morphology of             transplanted cells within the context of the surrounding             cerebellar tissue was evaluated after 3 weeks.             Naive P19 cells engrafted and survived in the cerebellum             of 7 of the 22 adult mice (survival rate 31.8 %).             Neuroprogenitors survived in 13 of the 21 mice (survival             rate was 61.9 %). Since the cut-off is P < 0.05,             the difference is not statistically significant (P =             0.069). An expansive appearance of the graft was significantly             more frequent (P = 0.0047) in naive P19             cells than in neuroprogenitors. In mice in which the             grafts did not survive, no marks of grafted cells or             only fluorescing detritus were found. In conclusion,             this is the first study to track the fate and morphology             of embryonic carcinoma cells transplanted into             the cerebellum, confirming that neuroprogenitors             derived from embryonic carcinoma cells can settle in             the host tissue and differentiate according to the surrounding             conditions. With further validation, the             embryonic carcinoma cells could become a valuable             model with which to study the impact of cell therapy             on neurodegenerative diseases.
authors: IVF Centers Prof. Zech - Pilsen s. r. o., Czech Republic.
authors: Z
authors: Z
authors: Houdek
authors: J
authors: J
authors: Cendelín
authors: V
authors: V
authors: Kulda
authors: V
authors: V
authors: Babuška
authors: F
authors: F
authors: Vožeh
authors: J
authors: J
authors: Hatina
authors: M
authors: M
authors: Králíčková
authors: N H
authors: NH
authors: Zech
authors: I
authors: I
authors: Veselá
authors: J
authors: J
authors: Pacherník
authors: P
authors: P
authors: Uher
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology*; D017690:Cell Transplantation; D002531:Cerebellum / Q000166:cytology*; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D009474:Neurons / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*