title: Serum antibodies protect against intraperitoneal challenge with enterotoxigenic Escherichia coli.
abstract: To assess whether anticolonization factor antigen I (CFA/I) fimbriae antibodies (Abs) from enterotoxigenic Escherichia coli (ETEC) can protect against various routes of challenge, BALB/c mice were immunized with a live attenuated Salmonella vaccine vector expressing CFA/I fimbriae. Vaccinated mice elicited elevated systemic IgG and mucosal IgA Abs, unlike mice immunized with the empty Salmonella vector. Mice were challenged with wild-type ETEC by the oral, intranasal (i.n.), and intraperitoneal (i.p.) routes. Naïve mice did not succumb to oral challenge, but did to i.n. challenge, as did immunized mice; however, vaccinated mice were protected against i.p. ETEC challenge. Two intramuscular (i.m.) immunizations with CFA/I fimbriae without adjuvant conferred 100% protection against i.p. ETEC challenge, while a single 30 μg dose conferred 88% protection. Bactericidal assays showed that ETEC is highly sensitive to anti-CFA/I sera. These results suggest that parenteral immunization with purified CFA/I fimbriae can induce protective Abs and may represent an alternative method to elicit protective Abs for passive immunity to ETEC.
authors: Department of Immunology & Infectious Diseases, Montana State University, Bozeman, MT 59717-3610, USA.
authors: Xinghong
authors: X
authors: Yang
authors: Theresa
authors: T
authors: Thornburg
authors: Kathryn
authors: K
authors: Holderness
authors: Zhiyong
authors: Z
authors: Suo
authors: Ling
authors: L
authors: Cao
authors: Timothy
authors: T
authors: Lim
authors: Recep
authors: R
authors: Avci
authors: David W
authors: DW
authors: Pascual
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D001770:Blood Bactericidal Activity / Q000187:drug effects / Q000276:immunology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D036781:Fimbriae Proteins / Q000008:administration & dosage* / Q000276:immunology* / Q000302:isolation & purification; D018928:Immunity, Mucosal / Q000276:immunology; D007114:Immunization / Q000379:methods*; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology; D058248:Serum Bactericidal Antibody Assay
chemical_list: D000906:Antibodies; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022562:Salmonella Vaccines; C022924:colonization factor antigens; D036781:Fimbriae Proteins