title: Biocontrol of Fusarium head blight: interactions between Trichoderma and mycotoxigenic Fusarium.
abstract: Fusarium head blight (FHB) is a re-emerging wheat disease that causes extensive damage through direct losses in yield and quality due to the presence of damaged Fusarium kernels and their associated mycotoxins such as the trichothecene deoxynivalenol (DON). Biological control, including the treatment of crop residues with antagonists, in order to reduce pathogen inoculum of FHB, holds considerable promise. Ten Trichoderma isolates, previously selected for their ability to grow in the presence of DON, were preliminarily investigated as potential antagonists against Fusarium culmorum and F. graminearum mycotoxigenic strains in plate confrontation assays. The three Trichoderma isolates showing antibiosis and mycoparasitism were evaluated for their capacity to inhibit DON production by F. graminearum and F. culmorum on two natural substrates. The expression of some chitinase-encoding genes by the two best resulting Trichoderma strains, during interaction with F. culmorum and F. graminearum, was monitored. All investigated genes from chitinase subgroups A, B and the new subgroup C responded to mycoparasitic conditions and were upregulated before contact and/or when in contact with the host. T. gamsii 6085, the best antagonist, was finally used in a competition test against F. culmorum and F. graminearum on natural substrates, using a qPCR approach to evaluate its effect on the pathogen's growth and DON production in haulms and rice. This test confirmed the ability of T. gamsii 6085 to antagonize the pathogens on rice. On wheat haulms, an extreme oligotrophic environment, T. gamsii 6085 seemed to develop very poorly and the growth of both the pathogens was unaffected by the presence of the antagonist.
authors: Department of Tree Science, Entomology and Plant Pathology 'G. Scaramuzzi', Section of Plant Pathology, Faculty of Agriculture, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
authors: F
authors: F
authors: Matarese
authors: Department of Tree Science, Entomology and Plant Pathology 'G. Scaramuzzi', Section of Plant Pathology, Faculty of Agriculture, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
authors: S
authors: S
authors: Sarrocco
authors: Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Straße 1a/166-5, 1060 Wien, Austria.
authors: S
authors: S
authors: Gruber
authors: Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Straße 1a/166-5, 1060 Wien, Austria.
authors: V
authors: V
authors: Seidl-Seiboth
authors: Department of Tree Science, Entomology and Plant Pathology 'G. Scaramuzzi', Section of Plant Pathology, Faculty of Agriculture, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
authors: G
authors: G
authors: Vannacci
mesh_terms: D000898:Antibiosis; D005670:Fusarium / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D012988:Soil Microbiology; D014242:Trichoderma / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014908:Triticum / Q000382:microbiology
chemical_list: D009183:Mycotoxins