title: Wnt3a is involved in the early stage of miPSC and mESC haemopoietic differentiation.
abstract: The Wnt/β-catenin signalling pathway is important in regulating not only self-renewal of haemopoietic progenitors and stem cells but also haemopoietic differentiation of ESCs (embryonic stem cells). However, it is still not clear how it affects haemopoietic differentiation. We have used a co-culture system for haemopoietic differentiation of mouse ESCs and iPSCs (induced pluripotent stem cells) in which the Wnt3a gene-modified OP9 cell line is used as stromal cells. The number of both Flk1+ and CD41+ cells generated from both co-cultured mouse ESCs and mouse iPSCs increased significantly, which suggest that Wnt3a is involved in the early stages of haemopoietic differentiation of mouse ESCs and mouse iPSCs in vitro.
authors: Stem Cell and Regenerative Medicine Laboratory, Beijing Institute of Transfusion Medicine, Beijing, Peoples Republic of China.
authors: Wencheng
authors: W
authors: Zhang
authors: Hailei
authors: H
authors: Yao
authors: Sihan
authors: S
authors: Wang
authors: Shuangshuang
authors: S
authors: Shi
authors: Yang
authors: Y
authors: Lv
authors: Lijuan
authors: L
authors: He
authors: Xue
authors: X
authors: Nan
authors: Wen
authors: W
authors: Yue
authors: Yanhua
authors: Y
authors: Li
authors: Xuetao
authors: X
authors: Pei
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006413:Hematopoietic System; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D060509:Wnt3A Protein / Q000378:metabolism*
chemical_list: D060509:Wnt3A Protein