title: Root extract of Anacyclus pyrethrum ameliorates seizures, seizure-induced oxidative stress and cognitive impairment in experimental animals.
abstract: In Ayurveda, Anacyclus pyrethrum has been used as a brain tonic. The present study evaluates the effect of hydroalcoholic extract of A. pyrethrum (HEAP) root against seizures, seizure-induced oxidative stress and cognitive impairment in experimental models of seizures. Male Wistar rats were used in the study. HEAP was administered in doses of 50, 100, 250, 500 in pentylenetetrazole (PTZ) model and 250, 500 and 1000 mg/kg in maximal electroshock (MES) model. Myoclonic jerk latency and generalized tonic clonic seizures (GTCS) were noted in PTZ whereas occurrence of tonic hind limb extension (THLE) was observed in MES seizures. Cognitive deficit was assessed using elevated plus maze and passive avoidance tests. Whole brain reduced glutathione, malondialdehyde levels and cholinesterase activity were measured. HEAP showed 50, 66.7, 83.3 and 100% protection at 50,100, 250 and 500 mg/kg, respectively against GTCS in PTZ induced seizures. In MES induced seizures, HEAP produced 16.7, 33.3 and 50% protection against THLE at 250, 500 and 1000 mg/kg, respectively. HEAP administration significantly prevented seizure induced oxidative stress and cognitive impairment in a dose-dependent manner. HEAP also normalized the decrease in cholinesterase activity caused by seizures. Thus, HEAP showed protective effect against seizures, seizure-induced oxidative stress and cognitive impairment in rats.
authors: Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors: Monika
authors: M
authors: Pahuja
authors: Jogender
authors: J
authors: Mehla
authors: K H
authors: KH
authors: Reeta
authors: Sujata
authors: S
authors: Joshi
authors: Yogendra Kumar
authors: YK
authors: Gupta
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D011723:Chrysanthemum cinerariifolium; D003072:Cognition Disorders / Q000209:etiology*; D004195:Disease Models, Animal; D004228:Dithionitrobenzoic Acid / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004597:Electroshock / Q000009:adverse effects; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010433:Pentylenetetrazole / Q000633:toxicity; D008517:Phytotherapy / Q000379:methods*; D028321:Plant Preparations / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000150:complications*
chemical_list: D028321:Plant Preparations; D008315:Malondialdehyde; D004228:Dithionitrobenzoic Acid; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D005978:Glutathione; D010433:Pentylenetetrazole