title: Engineering designer nucleases with customized cleavage specificities.
abstract: Engineered designer nucleases can be used to efficiently modify genomic sequence in a wide variety of model organisms and cell types. Zinc finger nucleases (ZFNs), consisting of an engineered zinc finger array fused to a non-specific cleavage domain, have been extensively used to modify a broad range of endogenous genes. Protocols for engineering ZFNs targeted to specific gene sequences of interest using the context-dependent assembly (CoDA) method are described in this unit.
authors: Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
authors: Jeffry D
authors: JD
authors: Sander
authors: Morgan L
authors: ML
authors: Maeder
authors: J Keith
authors: JK
authors: Joung
mesh_terms: D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D011485:Protein Binding; D013379:Substrate Specificity; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004247:DNA; D003851:Deoxyribonucleases