title: Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide.
abstract: Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and generally considered to be a saprophyte, but it is also an important opportunistic human pathogen. Pseudomonas aeruginosa elaborates a variety of virulence factors, one of which is lipopolysaccharide (LPS). LPS of P. aeruginosa is composed of three distinct regions: lipid A, core oligosaccharide (OS), and the long-chain O antigen. The core OS of P. aeruginosa is composed of L-glycero-D-manno-heptose, 3-deoxy-D-manno-oct-2-ulosonic acid, D-galactosamine, D-glucose, and L-rhamnose. Non-carbohydrate substituents are also found in the core OS including phosphate, 2-aminoethyl (di)phosphate, acetyl, alanyl and carbamoyl groups. Pseudomonas aeruginosa simultaneously synthesizes two core glycoforms, namely, capped and uncapped core. The capped core is covalently attached to an O antigen, whereas the uncapped core is devoid of O antigen. Although the core of P. aeruginosa LPS is relatively conserved, strain-to-strain variability of its structure exists. This includes phosphorylation pattern, the level of O-acetylation, and the presence or absence of a fourth glucose residue at the distal end of the uncapped core. A number of studies have been reported on the structures of unique truncated core OS with unusual modifications. This mini-review summarizes the diversity of P. aeruginosa complete and truncated core OS structures published over the past fifteen years.
authors: Department of Molecular and Cellular Biology, University of Guelph, Ontario, Canada.
authors: D
authors: D
authors: Kocíncová
authors: J S
authors: JS
authors: Lam
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D008050:Lipid A; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000235:genetics; D010766:Phosphorylation; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides