title: Involvement of GPR12 in the induction of neurite outgrowth in PC12 cells.
abstract: GPR12, an orphan G protein-coupled receptor, constitutively activates the Gs signaling pathway and further increases intracellular cyclic AMP. GPR12 overexpression has been reported to promote neurite extension in neurons or transform neuro2a neuroblastoma cells into neuron-like cells. However, the possible effects and mechanisms of GPR12 in the differentiation of PC12 cells are still unknown. The present study shows that GPR12 overexpression induced PC12 cells differentiation into neuron-like cells with enlarged cell sizes and neuritogenesis possibly via activation of Erk1/2 signaling and significantly increased the expression of several neurite outgrowth-related genes, including Bcl-xL, Bcl-2 and synaptophysin. These findings indicate that GPR12 may play a role in neurite outgrowth during PC12 cell differentiation.
authors: Advanced Institutes for Interdisciplinary Research, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
authors: Xiaoming
authors: X
authors: Lu
authors: Ning
authors: N
authors: Zhang
authors: Suzhen
authors: S
authors: Dong
authors: Yinghe
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology; D016716:PC12 Cells / Q000502:physiology* / Q000648:ultrastructure*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494813:Bcl2l1 protein, rat; C572161:GPR12 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D043562:Receptors, G-Protein-Coupled; D051020:bcl-X Protein