title: An electrochemical platform for acetylcholinesterase activity assay and inhibitors screening based on Michael addition reaction between thiocholine and catechol-terminated SAMs.
abstract: An electrochemical platform for acetylcholinesterase (AChE) activity assay and its inhibitors screening is developed based on the Michael addition reaction of thiocholine, the hydrolysis product of acetylthiocholine (AsCh) in the presence of AChE, with the electrogenerated o-quinone of catechol-terminated SAMs on a gold electrode. For understanding and confirming the mechanism of the reaction, the electrochemical behaviors of Michael addition reaction of two model compounds, cysteine (CYS) and glutathione (GSH), towards the catechol-terminated SAMs have been studied. The enzyme kinetics and the inhibition effects of three types of AChE inhibitors, which are tacrine, carbofuran and parathion-methyl, have been investigated using an amperometric method. Among these three inhibitors, tacrine exhibits the strongest inhibiting effect, which is reinforced by the resulting data of kinetic studies on each inhibitor's influence upon the enzyme activity.
authors: Department of Chemistry, East China Normal University, Shanghai 200062, China.
authors: Yuan
authors: Y
authors: Tian
authors: Siqiu
authors: S
authors: Ye
authors: Xinhao
authors: X
authors: Shi
authors: Li
authors: L
authors: Jing
authors: Cong
authors: C
authors: Liang
authors: Yuezhong
authors: Y
authors: Xian
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000122:Acetylthiocholine / Q000378:metabolism; D015374:Biosensing Techniques; D002235:Carbofuran / Q000494:pharmacology*; D002396:Catechols / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006046:Gold; D008743:Methyl Parathion / Q000494:pharmacology*; D013619:Tacrine / Q000494:pharmacology*; D013860:Thiocholine / Q000737:chemistry
chemical_list: D002396:Catechols; D002800:Cholinesterase Inhibitors; D008743:Methyl Parathion; D000122:Acetylthiocholine; D013619:Tacrine; D013860:Thiocholine; D006046:Gold; D000110:Acetylcholinesterase; D005978:Glutathione; D003545:Cysteine; C034221:catechol; D002235:Carbofuran