title: Endogenous RNA cleavages at the ribosomal SRL site likely reflect miRNA (miR) mediated translational suppression.
abstract: We previously suggested a mechanism whereby the RNA induced silencing complex (RISC) brings about a specific cleavage at the sarcin-ricin loop (SRL) of 28S ribosomal RNA thereby eliciting translational suppression. Here we experimentally show that endogenous cleavages take place at the SRL site, in both mammalian cells and in Caenorhabditis elegans. Furthermore we demonstrate that bulged and looped-out residues present in the imperfect miRNA-[mRNA target site] duplexes, are complementary to the SRL site. These results support, and are compatible with, our described mechanism whereby microRNAs mediate cleavage of the highly conserved 28S rRNA sarcin/ricin loop leading to translational suppression.
authors: Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel.
authors: Edward
authors: E
authors: Pichinuk
authors: Limor
authors: L
authors: Broday
authors: Daniel H
authors: DH
authors: Wreschner
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics; D045744:Cell Line, Tumor; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D059367:RNA Cleavage; D012339:RNA, Ribosomal, 28S / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D035683:MicroRNAs; D012339:RNA, Ribosomal, 28S; C014801:alpha-sarcin; D004722:Endoribonucleases