title: Methylomonas koyamae sp. nov., a type I methane-oxidizing bacterium from floodwater of a rice paddy field.
abstract: A novel methane-oxidizing bacterium, strain Fw12E-Y(T), was isolated from floodwater of a rice paddy field in Japan. Cells of strain Fw12E-Y(T) were Gram-negative, motile rods with a single polar flagellum and type I intracytoplasmic membrane arrangement. The strain grew only on methane or methanol as sole carbon and energy source. It was able to grow at 10-40 °C (optimum 30 °C), at pH 5.5-7.0 (optimum 6.5) and with 0-0.1% (w/w) NaCl (no growth above 0.5% NaCl). 16S rRNA gene sequence analysis showed that strain Fw12E-Y(T) is related most closely to members of the genus Methylomonas, but at low levels of similarity (95.0-95.4%). Phylogenetic analysis of pmoA and mxaF genes indicated that the strain belongs to the genus Methylomonas (97 and 92 % deduced amino acid sequence identities to Methylomonas methanica S1(T), respectively). The DNA G+C content of strain Fw12E-Y(T) was 57.1 mol%. Chemotaxonomic data regarding the major quinone (MQ-8) and major fatty acids (C(16:1) and C(14:0)) also supported its affiliation to the genus Methylomonas. Based on phenotypic, genomic and phylogenetic data, strain Fw12E-Y(T) is considered to represent a novel species of the genus Methylomonas, for which the name Methylomonas koyamae sp. nov. is proposed. The type strain is Fw12E-Y(T) ( = JCM 16701(T) = NBRC 105905(T) = NCIMB 14606(T)).
authors: Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
authors: Takuya
authors: T
authors: Ogiso
authors: Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
authors: Chihoko
authors: C
authors: Ueno
authors: National Center for Scientific and Technological Research 8, 03 BP 7192 Ouagadougou, Burkina Faso.|Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
authors: Dayéri
authors: D
authors: Dianou
authors: Division of Environmental Research, EcoTopia Science Institute, Nagoya University, Furocho, Chikusa, Nagoya 464-8603, Japan.
authors: Tran Van
authors: TV
authors: Huy
authors: Division of Environmental Research, EcoTopia Science Institute, Nagoya University, Furocho, Chikusa, Nagoya 464-8603, Japan.
authors: Arata
authors: A
authors: Katayama
authors: Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
authors: Makoto
authors: M
authors: Kimura
authors: Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
authors: Susumu
authors: S
authors: Asakawa
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008697:Methane / Q000378:metabolism; D020620:Methylomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D008697:Methane