title: Birch inner bark procyanidins can be resolved with enhanced sensitivity by hydrophilic interaction HPLC-MS.
abstract: A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
authors: Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, Turku, Finland. maarit.karonen@utu.fi
authors: Maarit
authors: M
authors: Karonen
authors: Jaana
authors: J
authors: Liimatainen
authors: Jari
authors: J
authors: Sinkkonen
mesh_terms: D029662:Betula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D044945:Proanthocyanidins / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010936:Plant Extracts; D044945:Proanthocyanidins