title: Thrombin-induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells.
abstract: Glioblastoma multiforme is one of the most common and aggressive tumors in central nervous system. It often possesses characteristic necrotic lesions with hemorrhages, which increase the chances of exposure to thrombin. Thrombin has been known as a regulator of MMP-9 expression and cancer cell migration. However, the effects of thrombin on glioma cells have not been clearly understood. In the present study, influences of thrombin on glioma cell migration were examined using Boyden chamber migration assay and thrombin-induced changes in MMP-9 expression were measured using zymography, semi-quantitative RT-PCR, and Western blotting. Furthermore, underlying signaling pathways by which thrombin induces MMP-9 expression were examined. Thrombin-induced migration and MMP-9 expression were significantly potentiated in the presence of wortmannin, a PI3K inhibitor, whereas MAPK inhibitors suppressed thrombin-induced migration and MMP-9 expression in C6 glioma cells. The present data strongly demonstrate that MAPK and PI3K pathways evidently regulate thrombin-induced migration and MMP-9 expression of C6 glioma cells. Therefore, the control of these pathways might be a beneficial therapeutic strategy for treatment of invasive glioblastoma multiforme.
authors: Department of Pharmacology, College of Medicine, Kangwon National University, Kangwon 200-701, Korea.
authors: Jiyoung
authors: J
authors: Kim
authors: Jae-Won
authors: JW
authors: Lee
authors: Song-In
authors: SI
authors: Kim
authors: Yong-Joon
authors: YJ
authors: Choi
authors: Won-Ki
authors: WK
authors: Lee
authors: Myung-Ja
authors: MJ
authors: Jeong
authors: Sang-Hoon
authors: SH
authors: Cha
authors: Hee Jae
authors: HJ
authors: Lee
authors: Wanjoo
authors: W
authors: Chun
authors: Sung-Soo
authors: SS
authors: Kim