title: Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.
abstract: Implementation of Xpert MTB/RIF requires quality assessment. A pilot program using dried culture spots (DCSs) of inactivated Mycobacterium tuberculosis is described. Of 274 DCS results received, 2.19% generated errors; the remainder yielded 100% correct Mycobacterium tuberculosis detection. The probe A cycle threshold (C(T)) variability of three DCS batches was ≤ 3.47. The study of longer-term DCS stability is ongoing.
authors: Department of Molecular Medicine and Haematology, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa. lesley.scott@nhls.ac.za
authors: L E
authors: LE
authors: Scott
authors: N
authors: N
authors: Gous
authors: B E
authors: BE
authors: Cunningham
authors: B D
authors: BD
authors: Kana
authors: O
authors: O
authors: Perovic
authors: L
authors: L
authors: Erasmus
authors: G J
authors: GJ
authors: Coetzee
authors: H
authors: H
authors: Koornhof
authors: W
authors: W
authors: Stevens
mesh_terms: D003951:Diagnostic Errors / Q000706:statistics & numerical data; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D010865:Pilot Projects; D011785:Quality Assurance, Health Care / Q000379:methods*; D012015:Reference Standards; D013019:South Africa; D014376:Tuberculosis / Q000175:diagnosis*