title: A DNA vaccine strategy for effective antibody induction to pathogen-derived antigens.
abstract: DNA-based vaccines are currently being developed for treating a diversity of human diseases including cancers, autoimmune conditions, allergies, and microbial infections. In this chapter, we present a general protocol that can be used as a starting point for developing DNA vaccines to pathogen-derived antigens, using Neisseria meningitidis as an example. In addition, we describe a fusion gene-based vaccine protocol for increasing the potency of DNA vaccines that are based on poorly immunogenic antigens such as short pathogen-derived polypeptides. Finally, we provide a safe and effective protocol for delivery of DNA vaccines, based on intramuscular injection followed by electroporation.
authors: Division of Cancer Sciences, Genetic Vaccines Group, University of Southampton Medical School, Southampton, UK. J.Rice@soton.ac.uk
authors: Jason
authors: J
authors: Rice
authors: Myron
authors: M
authors: Christodoulides
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D016503:Drug Delivery Systems / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D018274:Electroporation; D006801:Humans; D007273:Injections, Intramuscular; D008589:Meningococcal Infections / Q000517:prevention & control*; D009345:Neisseria meningitidis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000942:Antigens, Bacterial; D019444:Vaccines, DNA