title: Beneficial effects of THSG on acetic acid-induced experimental colitis: involvement of upregulation of PPAR-γ and inhibition of the Nf-Κb inflammatory pathway.
abstract: The polyphenolic compound 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) has been shown to possess anti-inflammatory effects. Here, we examined the effects of THSG on experimental mice with colitis induced by acetic acid and whether the underlying mechanisms were associated with the PPAR-γ and NF-κB pathways. Mice were randomized into six equal groups: normal, colitis model, THSG (10, 30, 60 mg·kg(-1)) and mesalazine. The mice were administered 10, 30, 60 mg·kg(-1) THSG or 100 mg·kg-1 mesalazine or saline once daily by intragastric administration for 7 days after induction of colitis by acetic acid irrigation. THSG dramatically attenuated acetic acid-induced colon lesions, including reversing the body weight loss and improving histopathological changes. THSG apparently decreased the increase of malondialdehyde (MDA) which is a marker of lipid peroxidation. THSG appears to exert its beneficial effects on acetic acid-induced experimental colitis through upregulation of PPAR-γ mRNA and protein levels and inhibition of the NF-κB pathway, which in turn decreases the protein overexpression of the downstream inflammatory mediators TNF-α, IL-6 and COX-2. The effect of THSG 60 mg·kg(-1) on PPAR-γ mRNA expression was higher than that of mesalazine. THSG may thus be a promising new candidate or lead compound for the treatment of IBD.
authors: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Cheng
authors: C
authors: Zeng
authors: Jun-Hua
authors: JH
authors: Xiao
authors: Mu-Jun
authors: MJ
authors: Chang
authors: Jia-Ling
authors: JL
authors: Wang
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D052246:Cyclooxygenase 2 Inhibitors; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005960:Glucosides / Q000008:administration & dosage / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D019804:Mesalamine / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D015850:Interleukin-6; D016328:NF-kappa B; D047495:PPAR gamma; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D019804:Mesalamine; D008315:Malondialdehyde; D051546:Cyclooxygenase 2; D019342:Acetic Acid