title: CD4+ T-cell deficiency in HIV patients responding to antiretroviral therapy is associated with increased expression of interferon-stimulated genes in CD4+ T cells.
abstract: Most patients with human immunodeficiency virus (HIV) who remain CD4(+) T-cell deficient on antiretroviral therapy (ART) exhibit marked immune activation. As CD4(+) T-cell activation may be mediated by microbial translocation or interferon-alpha (IFN-α), we examined these factors in HIV patients with good or poor CD4(+) T-cell recovery on long-term ART. Messenger RNA levels for 3 interferon-stimulated genes were increased in CD4(+) T cells of patients with poor CD4(+) T-cell recovery, whereas levels in patients with good recovery did not differ from those in healthy controls. Poor CD4(+) T-cell recovery was also associated with CD4(+) T-cell expression of markers of activation, senescence, and apoptosis, and with increased serum levels of the lipopolysaccharide receptor and soluble CD14, but these were not significantly correlated with expression of the interferon-stimulated genes. Therefore, CD4(+) T-cell recovery may be adversely affected by the effects of IFN-α, which may be amenable to therapeutic intervention.
authors: School of Pathology and Laboratory Medicine, University of Western Australia, Perth, Australia. sonia.fernandez@uwa.edu.au
authors: Sonia
authors: S
authors: Fernandez
authors: Sara
authors: S
authors: Tanaskovic
authors: Karla
authors: K
authors: Helbig
authors: Reena
authors: R
authors: Rajasuriar
authors: Marit
authors: M
authors: Kramski
authors: John M
authors: JM
authors: Murray
authors: Michael
authors: M
authors: Beard
authors: Damian
authors: D
authors: Purcell
authors: Sharon R
authors: SR
authors: Lewin
authors: Patricia
authors: P
authors: Price
authors: Martyn A
authors: MA
authors: French
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D017209:Apoptosis; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018959:CD57 Antigens / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003430:Cross-Sectional Studies; D005260:Female; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D015658:HIV Infections / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008070:Lipopolysaccharides / Q000097:blood; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins; D014157:Transcription Factors / Q000097:blood / Q000187:drug effects / Q000235:genetics; D019014:fas Receptor / Q000097:blood
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D044966:Anti-Retroviral Agents; D018959:CD57 Antigens; D006684:HLA-DR Antigens; C484290:IFIT1 protein, human; D016898:Interferon-alpha; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D019014:fas Receptor