title: Antibacterial characteristics and activity of water-soluble chitosan derivatives prepared by the Maillard reaction.
abstract: The antibacterial activity of water-soluble chitosan derivatives prepared by Maillard reactions against Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Escherichia coli, Shigella dysenteriae, and Salmonella typhimurium was examined. Relatively high antibacterial activity against various microorganisms was noted for the chitosan-glucosamine derivative as compared to the acid-soluble chitosan. In addition, it was found that the susceptibility of the test organisms to the water-soluble chitosan derivative was higher in deionized water than in saline solution. Metal ions were also found to reduce the antibacterial activity of the water-soluble chitosan derivative on S. aureus. The marked increase in glucose level, protein content and lactate dehydrogenase (LDH) activity was observed in the cell supernatant of S. aureus exposed to the water-soluble chitosan derivative in deionized water. The results suggest that the water-soluble chitosan produced by Maillard reaction may be a promising commercial substitute for acid-soluble chitosan.
authors: Department of Biological Science and Technology, China University of Science and Technology, Taipei 11581, Taiwan.
authors: Ying-Chien
authors: YC
authors: Chung
authors: Jan-Ying
authors: JY
authors: Yeh
authors: Cheng-Fang
authors: CF
authors: Tsai
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D015169:Colony Count, Microbial; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects; D015416:Maillard Reaction; D008826:Microbial Sensitivity Tests; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects; D012762:Shigella dysenteriae / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D004247:DNA; D048271:Chitosan; D007770:L-Lactate Dehydrogenase