title: Biglycan expression correlates with aggressiveness and poor prognosis of gastric cancer.
abstract: Biglycan, a member of the small leucine-rich proteoglycan family, has been implicated in the development and progression of human cancers. However, the clinical significance of biglycan expression in gastric cancer has not been determined. In the present study, biglycan mRNA and protein concentrations were analyzed using quantitative realtime reverse transcription polymerase chain reaction and Western blot in 69 gastric cancer and adjacent non-tumorous tissues, respectively. Biglycan expression was further assessed using immunohistochemistry in tissue microarrays that contained 264 cases of gastric cancer, and others containing normal or metastasized lymph node tumor tissues. Biglycan was upregulated at the transcriptional and translational levels and there was a correlation between the expression of biglycan mRNA and protein (P = 0.000, κ = 0.769). Over-expression of biglycan was strongly associated with lymph node metastasis, tumor (T) classification, metastasis (M) classification, vascular invasion and Union for International Cancer Control (UICC) stage. Patients with biglycan-positive tumors had a significantly higher disease recurrence rate and poorer survival than patients with biglycan-negative tumors after the radical surgery. Multivariate analysis revealed that biglycan expression is an independent prognostic indicator for survival of patients with gastric cancer. The data from the current study demonstrate that elevated expression of biglycan may play an important role in the development and progression of gastric cancer, and could be further evaluated as a biomarker for predication of a poor clinical outcome.
authors: Department of General Surgery, Shandong University Affiliated Qianfoshan Hospital, 16766 Jingshi Road, Jinan 250014, China.
authors: Bin
authors: B
authors: Wang
authors: Guang-Xin
authors: GX
authors: Li
authors: Shu-Guang
authors: SG
authors: Zhang
authors: Quan
authors: Q
authors: Wang
authors: Yu-Gang
authors: YG
authors: Wen
authors: Hua-Mei
authors: HM
authors: Tang
authors: Chong-Zhi
authors: CZ
authors: Zhou
authors: Ai-Yan
authors: AY
authors: Xing
authors: Jun-Wei
authors: JW
authors: Fan
authors: Dong-Wang
authors: DW
authors: Yan
authors: Guo-Qiang
authors: GQ
authors: Qiu
authors: Zhen-Hai
authors: ZH
authors: Yu
authors: Zhi-Hai
authors: ZH
authors: Peng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D058578:Biglycan / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D058578:Biglycan; D012333:RNA, Messenger