title: RhoA distribution in renal caveolar fractions in experimental type 1 diabetes.
abstract: Caveolae act as signalling platforms serving             as concentrating points for numerous signalling             molecules, as well as regulating flux through many             distinct signalling cascades. RhoA proteins have been             identified as potential actors in the pathophysiology             of the cardiovascular system. We used sucrose gradient             fractionation and immunoblotting to determine             caveolin-1 and RhoA presence in the kidney cortex of             streptozotocin-induced T1 diabetes rats (4-week duration),             and of diabetic rats treated with angiotensin             receptor blocker losartan (4 weeks, 20 mg/kg/day) to             retard renal hypertension. Positive RhoA/caveolin-1             co-immunoprecipitation result was detected in the             caveolar fraction that corresponded to the light-scattering             band obtained from diabetic rats, compared             to negative co-immunoprecipitation result in the caveolar             fraction obtained from control rats. The detection             of RhoA protein in the caveolar fractions and             the prospective RhoA/caveolin-1 association can be             used to examine the role of these signalling reactions             in the pathophysiology of microvascular complications             in type 1 diabetes.
authors: Diabetes Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
authors: H
authors: H
authors: Demová
authors: M
authors: M
authors: Cerná
mesh_terms: D057911:Angiotensin Receptor Antagonists / Q000627:therapeutic use; D000818:Animals; D015153:Blotting, Western; D021941:Caveolae / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism*; D047468:Immunoprecipitation; D019808:Losartan / Q000627:therapeutic use; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D057911:Angiotensin Receptor Antagonists; D051242:Caveolin 1; D020742:rhoA GTP-Binding Protein; D019808:Losartan