title: Anti-diabetic activity of a mineraloid isolate, in vitro and in genetically diabetic mice.
abstract: Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.
authors: Sunny BioDiscovery, Santa Paula, CA, USA.
authors: Joel
authors: J
authors: Deneau
authors: Taufeeq
authors: T
authors: Ahmed
authors: Roger
authors: R
authors: Blotsky
authors: Krzysztof
authors: K
authors: Bojanowski
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003032:Coal Mining / Q000191:economics; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000178:diet therapy*; D019587:Dietary Supplements; D020869:Gene Expression Profiling; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000032:analysis; D006801:Humans; D006943:Hyperglycemia / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D051379:Mice; D016688:Mice, Inbred NOD; D008903:Minerals / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012322:RNA Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism; D015854:Up-Regulation; D015430:Weight Gain
chemical_list: D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D007220:Industrial Waste; D008903:Minerals; D024101:Mitochondrial Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; C558406:HbA(1c) protein, mouse; C568542:leonardite