title: Rapid finding and quantification of the major antioxidant in water extracts of three marine drug organisms from China by online HPLC-DAD/MS-DPPH.
abstract: A method based on high-performance liquid chromatography (HPLC) with diode array detector coupled with electrospray ionisation-mass spectrometry and an online detection system for radical scavenging was established and used to rapidly find and quantify antioxidant compounds in the water extracts of Hippocampus japonicus Kaup, Hippocampus kuda Bleeker and Syngnathus acus Linnaeus. The online screening results revealed the presence of one major radical scavenging compound identified as hypoxanthine by comparison of mass data and retention time with the standard. Subsequently, the developed HPLC method was applied to quantify hypoxanthine in different H. japonicus, H. kuda and S. acus samples. The results indicated that the developed HPLC method is simple and reliable for the quantification of hypoxanthine with a detection limit at 0.002 µg  mL(-1), and a high recovery from 96.3% to 102.1%. This method provides a powerful tool for rapid identification and quantification of free radical scavenging compounds in complex marine natural products.
authors: Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, SOA 266061, Qingdao, China.
authors: Li
authors: L
authors: Zheng
authors: Junhui
authors: J
authors: Chen
authors: Hengqiang
authors: H
authors: Zhao
authors: Qian
authors: Q
authors: Shi
authors: Baijuan
authors: B
authors: Yang
authors: Hongyan
authors: H
authors: Cheng
authors: Jiaye
authors: J
authors: Zang
authors: Xiaoru
authors: X
authors: Wang
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008386:Marine Biology; D013058:Mass Spectrometry / Q000379:methods*; D014867:Water
chemical_list: D000975:Antioxidants; D014867:Water