title: DNA-encoded signal conversion for sensitive microgravimetric detection of small molecule-protein interaction.
abstract: Identification and quantification of small organic molecules capable of binding to a protein of interest with reasonable affinity and specificity is a central problem. Via developing DNA-encoded recognizing probe, we validate a proof-of-principle for constructing of small target-to-DNA conversion that screens the small molecule-protein interaction. Successful identification of β-indole acetic acid, abscisic acid, or 2,4-dichlorophenoxyacetic acid/corresponding antibody binding implies its fascinating potential for interrogating small molecule/protein interaction.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha, P R China.
authors: Yue-Hua
authors: YH
authors: Fei
authors: Dengyou
authors: D
authors: Liu
authors: Zai-Sheng
authors: ZS
authors: Wu
authors: Guo-Li
authors: GL
authors: Shen
authors: Ru-Qin
authors: RQ
authors: Yu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000494:pharmacology*
chemical_list: D015342:DNA Probes; D011506:Proteins; D054852:Small Molecule Libraries; D004247:DNA