title: HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable element silencing in Arabidopsis.
abstract: The molecular mechanism of how the histone deacetylase HDA6 participates in maintaining transposable element (TE) silencing in Arabidopsis (Arabidopsis thaliana) is not yet defined. In this study, we show that a subset of TEs was transcriptionally reactivated and that TE reactivation was associated with elevated histone H3 and H4 acetylation as well as increased H3K4Me3 and H3K4Me2 in hda6 mutants. Decreased DNA methylation of the TEs was also detected in hda6 mutants, suggesting that HDA6 silences the TEs by regulating histone acetylation and methylation as well as the DNA methylation status of the TEs. Similarly, transcripts of some of these TEs were also increased in the methyltransferase1 (met1) mutant, with decreased DNA methylation. Furthermore, H4 acetylation, H3K4Me3, H3K4Me2, and H3K36Me2 were enriched at the coregulated TEs in the met1 and hda6 met1 mutants. Protein-protein interaction analysis indicated that HDA6 physically interacts with MET1 in vitro and in vivo, and further deletion analysis demonstrated that the carboxyl-terminal region of HDA6 and the bromo-adjacent homology domain of MET1 were responsible for the interaction. These results suggested that HDA6 and MET1 interact directly and act together to silence TEs by modulating DNA methylation, histone acetylation, and histone methylation status.
authors: Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
authors: Xuncheng
authors: X
authors: Liu
authors: Chun-Wei
authors: CW
authors: Yu
authors: Jun
authors: J
authors: Duan
authors: Ming
authors: M
authors: Luo
authors: Koching
authors: K
authors: Wang
authors: Gang
authors: G
authors: Tian
authors: Yuhai
authors: Y
authors: Cui
authors: Keqiang
authors: K
authors: Wu
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004251:DNA Transposable Elements; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008745:Methylation; D009154:Mutation; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D006657:Histones; C499608:MET1 protein, Arabidopsis; D004248:DNA (Cytosine-5-)-Methyltransferases; C432452:AT5G63110 protein, Arabidopsis; D006655:Histone Deacetylases