title: Highly sensitive and selective DNA-based detection of mercury(II) with α-hemolysin nanopore.
abstract: The duplex formation mediated by Hg(2+) in a properly designed ssDNA generates a stable hairpin structure, which greatly alters the translocation profile of the ssDNA through α-hemolysin nanopore. From the 2D-events contour plot, the presence of Hg(2+) can be confirmed in as little as 30 min at ∼7 nM or higher. The sensor is highly selective to Hg(2+), without interference from other metal ions. It can be fabricated from readily available materials, without the processes of synthesis, purification, probe-making, and so forth. This sensing strategy opens new possibilities for detecting many types of analytes which have specific interactions with DNA molecules.
authors: Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors: Shuang
authors: S
authors: Wen
authors: Tao
authors: T
authors: Zeng
authors: Lei
authors: L
authors: Liu
authors: Kai
authors: K
authors: Zhao
authors: Yuliang
authors: Y
authors: Zhao
authors: Xianjin
authors: X
authors: Liu
authors: Hai-Chen
authors: HC
authors: Wu
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D058608:Nanopores
chemical_list: D004277:DNA, Single-Stranded; D006460:Hemolysin Proteins; D008628:Mercury