title: Streptomyces herbaceus sp. nov., Streptomyces incanus sp. nov. and Streptomyces pratens sp. nov., isolated from the soil of a hay meadow.
abstract: The taxonomic positions of three streptomycetes isolated from a soil sample from a hay meadow were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of the genus Streptomyces and, in phylogenetic analyses based on 16S rRNA gene sequences, formed a distinct subclade that was most closely related to the Streptomyces prasinus subclade. DNA-DNA relatedness studies showed that the novel strains belonged to three different genomic species. The novel strains could be distinguished from one another and from the type strains of the species classified in the S. prasinus subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the novel strains be assigned to the genus Streptomyces as Streptomyces herbaceus sp. nov., Streptomyces incanus sp. nov. and Streptomyces pratens sp. nov., with BK119(T) ( = KACC 21001(T)  = CGMCC 4.5797(T)), BK128(T) ( = KACC 21002(T)  = CGMCC 4.5799(T)) and BK138(T) ( = KACC 20904(T)  = CGMCC 4.5800(T)) as the respective type strains.
authors: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors: Byung-Yong
authors: BY
authors: Kim
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Xiaoying
authors: X
authors: Rong
authors: Departamento de Entomologia & Acarologia, ESALQ, Universidade de São Paulo, 13418-900 Piracicaba, Brazil.|School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors: Tiago D
authors: TD
authors: Zucchi
authors: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors: Avinash N V
authors: ANV
authors: Bonda
authors: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Ying
authors: Y
authors: Huang
authors: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors: Michael
authors: M
authors: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D006109:Poaceae; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D013045:Species Specificity; D013171:Spores, Bacterial / Q000648:ultrastructure; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil