title: In vitro fermentation by human gut bacteria of proteolytically digested caseinomacropeptide nonenzymatically glycosylated with prebiotic carbohydrates.
abstract: The in vitro fermentation selectivity of hydrolyzed caseinomacropeptide (CMP) glycosylated, via Maillard reaction (MR), with lactulose, galacto-oligosaccharides from lactose (GOSLa), and galacto-oligosaccharides from lactulose (GOSLu) was evaluated, using pH-controlled small-scale batch cultures at 37 °C under anaerobic conditions with human feces. After 10 and 24 h of fermentation, neoglyconjugates exerted a bifidogenic activity, similar to those of the corresponding prebiotic carbohydrates. No significant differences were found in  Bacteroides ,  Lactobacillus - Enterococcus ,  Clostridium histolyticum  subgroup,  Atopobium  and  Clostridium coccoides - Eubacterium rectale  populations. Concentrations of lactic acid and short-chain fatty acids (SCFA) produced during the fermentation of prebiotic carbohydrates were similar to those produced for their respective neoglycoconjugates at both fermentation times. These findings, joined with the functional properties attributed to CMP, could open up new applications of MR products involving prebiotics as novel multiple-functional ingredients with potential beneficial effects on human health.
authors: Instituto de Química Orgánica General, CSIC, 28006 Madrid, Spain.
authors: Oswaldo
authors: O
authors: Hernandez-Hernandez
authors: M Luz
authors: ML
authors: Sanz
authors: Sofia
authors: S
authors: Kolida
authors: Robert A
authors: RA
authors: Rastall
authors: F Javier
authors: FJ
authors: Moreno
mesh_terms: D000328:Adult; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002364:Caseins / Q000737:chemistry / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005260:Female; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D056692:Prebiotics / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D002364:Caseins; D010446:Peptide Fragments; D056692:Prebiotics; C065989:caseinomacropeptide