title: Direct detection of Staphylococcus osteoarticular infections by use of Xpert MRSA/SA SSTI real-time PCR.
abstract: We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. Using these rapid results, appropriate antibiotic treatment could be rapidly initiated.
authors: Laboratoire, Clinique de L'Union, Bd de Ratalens, 31240 St Jean, France. anne.dubouix@clinique-union.fr
authors: Anne
authors: A
authors: Dubouix-Bourandy
authors: Aymard
authors: A
authors: de Ladoucette
authors: Valerie
authors: V
authors: Pietri
authors: Nazim
authors: N
authors: Mehdi
authors: David
authors: D
authors: Benzaquen
authors: Régis
authors: R
authors: Guinand
authors: Jean-Marc
authors: JM
authors: Gandois
mesh_terms: D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010003:Osteoarthritis / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors