title: Self-assembled multivalent DNA nanostructures for noninvasive intracellular delivery of immunostimulatory CpG oligonucleotides.
abstract: Designed oligonucleotides can self-assemble into DNA nanostructures with well-defined structures and uniform sizes, which provide unprecedented opportunities for biosensing, molecular imaging, and drug delivery. In this work, we have developed functional, multivalent DNA nanostructures by appending unmethylated CpG motifs to three-dimensional DNA tetrahedra. These small-sized functional nanostructures are compact, mechanically stable, and noncytotoxic. We have demonstrated that DNA nanostructures are resistant to nuclease degradation and remain substantially intact in fetal bovine serum and in cells for at least several hours. Significantly, these functional nanostructures can noninvasively and efficiently enter macrophage-like RAW264.7 cells without the aid of transfection agents. After they are uptaken by cells, CpG motifs are recognized by the Toll-like receptor 9 (TLR9) that activates downstream pathways to induce immunostimulatory effects, producing high-level secretion of various pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-12. We also show that multivalent CpG motifs greatly enhance the immunostimulatory effect of the nanostructures. Given the high efficacy of these functional nanostructures and their noncytotoxic nature, we expect that DNA nanostructures will become a promising tool for targeted drug delivery.
authors: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors: Jiang
authors: J
authors: Li
authors: Hao
authors: H
authors: Pei
authors: Bing
authors: B
authors: Zhu
authors: Le
authors: L
authors: Liang
authors: Min
authors: M
authors: Wei
authors: Yao
authors: Y
authors: He
authors: Nan
authors: N
authors: Chen
authors: Di
authors: D
authors: Li
authors: Qing
authors: Q
authors: Huang
authors: Chunhai
authors: C
authors: Fan
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018899:CpG Islands; D004247:DNA / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D009841:Oligonucleotides / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000276:Adjuvants, Immunologic; D004337:Drug Carriers; D009841:Oligonucleotides; D004247:DNA