title: Carbonic anhydrase II autoantibody and oxidative stress in rheumatoid arthritis.
abstract: OBJECTIVE
  To investigate the relationship between carbonic anhydrase (CA) II autoantibody and lipid peroxidation, certain antioxidant parameters, and cytokines in rheumatoid arthritis (RA) patients.
  
  
  METHODS
  Serum levels of CA II autoantibody, cytokines (TNFα, IL-6, IFN-γ, IL-1β) and bone markers (crosslaps, osteocalcine) and erythrocyte levels of antioxidant enzyme activities (SOD, CAT, GPx), GSH and MDA, and CA activities were measured in RA patients and healthy controls.
  
  
  RESULTS
  The CA II autoantibody titers were significantly higher (P<0.05), and erythrocyte SOD activities were significantly lower (P<0.05) in RA patients. A significant negative correlation between CA II autoantibody titers and SOD activities in RA group was established (r=-0.430, p=0.006). The elevated cytokine levels could not be correlated with CA II autoantibody levels in RA.
  
  
  CONCLUSIONS
  These results suggest that increased erythrocyte oxidative stress observed in RA may be effective in the mechanism of CA II autoantibody formation.
authors: Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. alver61@yahoo.com
authors: Ahmet
authors: A
authors: Alver
authors: Ayşe
authors: A
authors: Şentürk
authors: Haşim
authors: H
authors: Çakirbay
authors: Ahmet
authors: A
authors: Menteşe
authors: Ferhat
authors: F
authors: Gökmen
authors: E Edip
authors: EE
authors: Keha
authors: Fahri
authors: F
authors: Uçar
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000276:immunology / Q000503:physiopathology; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D024402:Carbonic Anhydrase II / Q000097:blood / Q000276:immunology*; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D003094:Collagen / Q000097:blood; D016207:Cytokines / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D016207:Cytokines; D010446:Peptide Fragments; C110762:glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine; D015675:Osteocalcin; D008315:Malondialdehyde; D003094:Collagen; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024402:Carbonic Anhydrase II; D005978:Glutathione