title: A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin.
abstract: 11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.
authors: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China.
authors: Yu
authors: Y
authors: Wang
authors: Zhipu
authors: Z
authors: Luo
authors: Xiaoli
authors: X
authors: Shi
authors: Huan
authors: H
authors: Wang
authors: Lei
authors: L
authors: Nie
authors: Mingdong
authors: M
authors: Huang
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D003619:Dansyl Compounds / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D009226:Myristates / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003619:Dansyl Compounds; D005227:Fatty Acids; D005456:Fluorescent Dyes; D009226:Myristates; D012709:Serum Albumin; C054400:11-(dansylamino)undecanoic acid