title: Anaplasma phagocytophilum infection induces apoptosis in HL-60 cells.
abstract: Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intra-cellular bacterium that survives in neutrophils by delaying apoptosis. The human promyelocytic leukemia cell line HL-60 has been the ultimate choice for culturing Anaplasma in vitro. In this study, we assessed the various events of drug-induced apoptosis in A. phagocytophilum-infected HL-60 cells. Anaplasma infection reduced the cell viability and increased the apoptosis in HL-60 cells and staurosporine or etoposide-induced apoptosis was further exacerbated with Anaplasma infection. Altogether our results suggest that A. phagocytophilum infection is proapoptotic in HL-60 cells unlike in neutrophils where it is antiapoptotic.
authors: Inflammation Program and Division of Rheumatology, Department of Internal Medicine, University of Iowa and Veterans Administration Medical Center, 2501 Crosspark Road, MTF E104, Coralville, Iowa City, IA 52241, USA.
authors: Pratap
authors: P
authors: Karki
authors: Jacob W
authors: JW
authors: Ijdo