title: Stimulating effect of growth hormone on type IV collagen production by endothelial cells cultured in normal and high glucose.
abstract: Collagen IV accumulation is characteristic of diabetic angiopathy. To test the possible contribution of GH, we studied its effects on collagen IV production by human umbilical vein endothelial cells at 5.5 and 16.7 mmol/l glucose. GH (100 ng/ml) markedly increased collagen IV level in the culture supernatant and in the insoluble extracellular matrix and cell fraction at both glucose concentrations. This stimulating effect of GH was additional to that of high glucose. It was more pronounced on collagen IV than on total protein synthesis. GH increased free latent gelatinase activity slightly at normal and markedly at high glucose. Using GF109203X, a PKC inhibitor, we observed that high glucose, but not GH, activated PKC. These two factors stimulating collagen IV production appear to work through different pathways, favoring an additivity of their effects. This supports the contribution of high plasma GH in diabetic vascular basement membrane thickening.
authors: Equipe de Recherches sur la Biochimie et la Pharmacologie des Vaisseaux et du Rein, Protéines Modifiées, Protéases et Physiopathologie de l'Endothélium Vasculaire, Laboratoire de Pharmacologie, Faculté de Pharmacie, 4 avenue de l'Observatoire, 75006 Paris, France.
authors: A
authors: A
authors: Bakillah
authors: R
authors: R
authors: Guillot
authors: P
authors: P
authors: Urios
authors: A-M
authors: AM
authors: Grigorova-Borsos
authors: M
authors: M
authors: Sternberg
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media; D018093:Gelatinases / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000502:physiology*; D019382:Human Growth Hormone / Q000494:pharmacology / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D044967:Serum
chemical_list: D024141:Collagen Type IV; D003470:Culture Media; D007211:Indoles; D008301:Maleimides; D019382:Human Growth Hormone; D011392:Proline; D011493:Protein Kinase C; D018093:Gelatinases; D005947:Glucose; C070515:bisindolylmaleimide I