title: Multiresidue determination of carcinogenic polycyclic aromatic hydrocarbons in honey by solid-phase extraction and high-performance liquid chromatography.
abstract: An analytical procedure based on solid-phase extraction, using ethyl acetate as the elution solvent, and high-performance liquid chromatography with fluorescence and diode array detection was developed for the identification and quantification of polycyclic aromatic hydrocarbons (PAHs)  in honey. The method has been optimized and validated in accordance with Commission Regulation 333/2007 and Commission Decision 2002/657/EC. This method allows the identification of the 15 PAHs that should be monitored in food matrices, as proposed in 2002 by the Scientific Committee on Food  and later by the European Union in the Commission Recommendation 2005/108/EC, because of their genotoxic and carcinogenic properties. The results of the validation study were in agreement with quality criteria described in European legislation in terms of sensitivity, accuracy, and ruggedness,  and the method was applied to the analysis of 42 honey samples (21 from Spain and 21 from other regions). The honey samples were not contaminated by PAHs at detectable levels and thus could be marketed without health risk.
authors: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Facultad de Veterinaria, C/ Miguel Servet 177, 50013 Zaragoza, Spain. lourdesc@unizar.es
authors: Lourdes
authors: L
authors: Corredera
authors: Susana
authors: S
authors: Bayarri
authors: Consuelo
authors: C
authors: Pérez-Arquillué
authors: Regina
authors: R
authors: Lázaro
authors: Francisco
authors: F
authors: Molino
authors: Andantonio
authors: A
authors: Herrera
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons