title: Salinomycin can effectively kill ALDH(high) stem-like cells on gastric cancer.
abstract: Salinomycin is a novel identified cancer stem cells (CSCs) killer. Higher ALDH activity represents CSCs characterization. Here, we screened ALDH activities on several gastric cancer cell lines and divided them into ALDH(high) and ALDH(low) gastric cancer groups. ALDH(high) cancer cells (NCI-N87 and SNU-1) disclosed more CSCs characteristics, such as higher levels of Sox2, Nanog and Nestin, more floating spheroid bodies, more colony formation and more resistance to conventional chemotherapeutic drugs 5-Fu and CDDP, compared to these parameters observed in ALDH(low) cancer cells (P<0.01). Importantly, ALDH(high) cancer cells are relatively sensitive to salinomycin when compared to ALDH(low) cancer cells (P<0.01). Our results confirmed ALDH as functional marker of CSCs population on gastric cancer. Salinomycin might be selective therapy for CSCs fraction, which is resistant to conventional anticancer drugs 5-Fu and CDDP.
authors: Shanghai Key Laboratory of Diagnosis and Treatment for Gastric Cancer, Shanghai Jiao Tong University, School of Medicine, Ruijin er Road, No. 197, 200025 Shanghai, China.
authors: Qiao Ming
authors: QM
authors: Zhi
authors: Xue Hua
authors: XH
authors: Chen
authors: Jun
authors: J
authors: Ji
authors: Jia Nian
authors: JN
authors: Zhang
authors: Jian Fang
authors: JF
authors: Li
authors: Qu
authors: Q
authors: Cai
authors: Bing Ya
authors: BY
authors: Liu
authors: Qin Long
authors: QL
authors: Gu
authors: Zheng Gang
authors: ZG
authors: Zhu
authors: Ying Yan
authors: YY
authors: Yu
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D000080924:Aldehyde Dehydrogenase 1 Family; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics; D000071317:Nanog Homeobox Protein; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D064231:Nestin; D011714:Pyrans / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D050697:Retinal Dehydrogenase; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D018874:Spheroids, Cellular / Q000187:drug effects; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D014410:Tumor Stem Cell Assay; D000095662:Polyether Polyketides
chemical_list: D000444:Aldehyde Dehydrogenase; D000080924:Aldehyde Dehydrogenase 1 Family; D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D018906:Antineoplastic Agents, Alkylating; D002945:Cisplatin; D005472:Fluorouracil; D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D000071317:Nanog Homeobox Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin; D011714:Pyrans; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D050697:Retinal Dehydrogenase; D055748:SOXB1 Transcription Factors; C486780:NANOG protein, human; C577484:NES protein, human; C527782:SOX2 protein, human; C010327:salinomycin; D000095662:Polyether Polyketides; C510223:ALDH1A1 protein, human