title: Structure and catalysis by carbonic anhydrase II: role of active-site tryptophan 5.
abstract: The tryptophan residue Trp5, highly conserved in the α class of carbonic anhydrases including human carbonic anhydrase II (HCA II), is positioned at the entrance of the active site cavity and forms a π-stacking interaction with the imidazole ring of the proton shuttle His64 in its outward orientation. We have observed that replacement of Trp5 in HCA II caused significant structural changes, as determined by X-ray diffraction, in the conformation of 11 residues at the N-terminus and in the orientation of the proton shuttle residue His64. Most significantly, two variants W5H and W5E HCA II had His64 predominantly outward in orientation, while W5F and wild type showed the superposition of both outward and inward orientations in crystal structures. Although Trp5 influences the orientation of the proton shuttle His64, this orientation had no significant effect on the rate constant for proton transfer near 1μs(-1), determined by exchange of (18)O between CO(2) and water measured by mass spectrometry. The apparent values of the pK(a) of the zinc-bound water and the proton shuttle residue suggest that different active-site conformations influence the two stages of catalysis, the proton transfer stage and the interconversion of CO(2) and bicarbonate.
authors: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, United States.
authors: Rose
authors: R
authors: Mikulski
authors: John F
authors: JF
authors: Domsic
authors: George
authors: G
authors: Ling
authors: Chingkuang
authors: C
authors: Tu
authors: Arthur H
authors: AH
authors: Robbins
authors: David N
authors: DN
authors: Silverman
authors: Robert
authors: R
authors: McKenna
mesh_terms: D024402:Carbonic Anhydrase II / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002384:Catalysis; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D006639:Histidine / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D006639:Histidine; D014364:Tryptophan; D024402:Carbonic Anhydrase II