title: Identification and molecular characterization of an Alba-family protein from human malaria parasite Plasmodium falciparum.
abstract: We have investigated the DNA-binding nature as well as the function of a putative Alba (Acetylation lowers binding affinity) family protein (PfAlba3) from Plasmodium falciparum. PfAlba3 possesses DNA-binding property like Alba family proteins. PfAlba3 binds to DNA sequence non-specifically at the minor groove and acetylation lowers its DNA-binding affinity. The protein is ubiquitously expressed in all the erythrocytic stages of P. falciparum and it exists predominantly in the acetylated form. PfAlba3 inhibits transcription in vitro by binding to DNA. Plasmodium falciparum Sir2 (PfSir2A), a nuclear localized deacetylase interacts with PfAlba3 and deacetylates the lysine residue of N-terminal peptide of PfAlba3 specific for DNA binding. PfAlba3 is localized with PfSir2A in the periphery of the nucleus. Fluorescence in situ hybridization studies revealed the presence of PfAlba3 in the telomeric and subtelomeric regions. ChIP and ChIP ReChIP analyses further confirmed that PfAlba3 binds to the telomeric and subtelomeric regions as well as to var gene promoter.
authors: Department of Infectious Diseases and Immunology, Indian Institute of Chemical Biology, Jadavpur, Kolkata 700032, West Bengal, India.
authors: Manish
authors: M
authors: Goyal
authors: Athar
authors: A
authors: Alam
authors: Mohd Shameel
authors: MS
authors: Iqbal
authors: Sumanta
authors: S
authors: Dey
authors: Samik
authors: S
authors: Bindu
authors: Chinmay
authors: C
authors: Pal
authors: Anindyajit
authors: A
authors: Banerjee
authors: Saikat
authors: S
authors: Chakrabarti
authors: Uday
authors: U
authors: Bandyopadhyay
mesh_terms: D000595:Amino Acid Sequence; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D010963:Plasmodium falciparum; D011485:Protein Binding; D015800:Protozoan Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry; D056565:Sirtuin 2 / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D010455:Peptides; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D004247:DNA; D056565:Sirtuin 2; D008239:Lysine