title: Comparative full-length sequence analysis of Marek's disease virus vaccine strain 814.
abstract: The complete DNA sequence of Marek's disease virus (MDV) serotype 1 vaccine strain 814 was determined. It consisted of 172,541 bp, with an overall gene organization identical to that of the MDV-1 type strains. Comparative genomic analysis of vaccine strains (814 and CVI988) and other strains (CU-2, Md5, and Md11) showed that 814 was most similar to CVI988. Several unique insertions, deletions, and substitutions were identified in strain 814. Of note, a 177-bp insertion in the overlapping genes encoding the Meq, RLORF6, and 23-kDa proteins of strain 814 was identified, and a 69-bp deletion was also located in the origin of replication site (Ori) in the gene encoding RLORF12. Compared to the CVI988 vaccine strain, a deletion of 510 bp was identified in the UL36 gene. These analyses identified key mutations in the 814 strain and the vaccine strain that could be exploited for future MDV vaccine design.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
authors: Feng
authors: F
authors: Zhang
authors: Chang-Jun
authors: CJ
authors: Liu
authors: Yan-Ping
authors: YP
authors: Zhang
authors: Zhi-Jie
authors: ZJ
authors: Li
authors: Ai-Ling
authors: AL
authors: Liu
authors: Fu-Hai
authors: FH
authors: Yan
authors: Feng
authors: F
authors: Cong
authors: Yun
authors: Y
authors: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D023281:Genomics; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000235:genetics*; D008380:Marek Disease / Q000821:virology*; D022501:Marek Disease Vaccines / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D017386:Sequence Homology, Amino Acid
chemical_list: D004279:DNA, Viral; D022501:Marek Disease Vaccines