title: [Effects of human cytomegalovirus on the cell cycle of duct epithelial cell cultures of human salivary gland in vitro and relative mechanism].
abstract: OBJECTIVE
  To investigate the effects of human cytomegalovirus (HCMV) on the cell cycle of duct epithelial cell cultures of human salivary gland (HSG) in vitro and relative mechanism.
  
  
  METHODS
  HSG was cultured in vitro. Reverse transcriptase polymerase chain reaction (RT-PCR) and nest-RT-PCR were used respectively to investigate ie1/ie2 transcription in HSG infected by human cytomegalovirus(HCMV). The effects of HCMV on the cell cycle of HSG were studied by flow cytometry in vitro. The expression of cyclin D1 in HSG infected by HCMV was detected by Western blotting.
  
  
  RESULTS
  HCMV iel/ie2 transcription could be detected in HSG infected by HCMV. HCMV arrested productively infected cells in G1 stage. And cyclin D1 was down-regulated in HCMV infected HSG.
  
  
  CONCLUSIONS
  HCMV inhibits proliferation of HSG by affecting G1/S check point and down-regulating cyclin D1 in vitro.
authors: Department of Pathology, The First Hospital of PLA, Lanzhou 730030, China.
authors: Guo-Rong
authors: GR
authors: Yang
authors: Gao-Sheng
authors: GS
authors: Huang
authors: Xiao-Xia
authors: XX
authors: He
authors: Yong-Jie
authors: YJ
authors: Xue
mesh_terms: D015153:Blotting, Western; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D003587:Cytomegalovirus / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology*; D005434:Flow Cytometry; D006801:Humans; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism
chemical_list: D019938:Cyclin D1