title: Utility of the capsule-based technique for cell block preparation - in body fluids and Liqui-PREP™ specimens.
abstract: OBJECTIVE
  The aim of this study was to find a better technique for the cell block preparation.
  
  
  METHODS
  We developed a novel capsule-based technique and applied it to different cell block preparation materials including plasma thromboplastin (PT) clot and agarose gel-embedding medium, in order to concentrate the cells in a limited field, then compared the result with the widely used, modified alcohol fixative preparation technique based on specimen adequacy and diagnostic accuracy. Twenty-eight nongynecologic body fluids and 41 gynecologic Liqui-PREP™ (LGM International Inc., Fort Lauderdale, Fla., USA) cytology specimens were collected for cell block preparation. We performed routine hematoxylin and eosin staining on the cell block sections, which were scored according to four specimen adequacy parameters: background, cellularity, nuclear preservation and cytoplasmic preservation. The differences between the diagnostic results of cell block slide and conventional cytology smear were also presented.
  
  
  RESULTS
  The capsule-based PT clot technique in nongynecologic body fluids provided more cellularity in the cell block sections and reduced atypical diagnosis when compared to conventional smears.
  
  
  CONCLUSIONS
  We suggest the capsule-based PT clot technique is a better technique for nongynecologic body fluids in the preparation of a satisfactory cell block.
authors: Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC.
authors: Chien-Hui
authors: CH
authors: Wen
authors: Shu-Chuan
authors: SC
authors: Tsao
authors: Yue-Chiu
authors: YC
authors: Su
authors: Chun-Chieh
authors: CC
authors: Wu
authors: Chee-Yin
authors: CY
authors: Chai
mesh_terms: D001826:Body Fluids / Q000166:cytology*; D003581:Cytodiagnosis; D003584:Cytological Techniques; D005260:Female; D016591:Histocytological Preparation Techniques / Q000706:statistics & numerical data*; D006801:Humans; D010996:Pleural Effusion / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D014626:Vaginal Smears