title: Comparison of in vitro biocompatibility of NanoBone(®)  and BioOss(®)  for human osteoblasts.
abstract: BACKGROUND
  Scaffolds for bone tissue engineering seeded with the patient's own cells might be used as a preferable method to repair bone defects in the future. With the emerging new technologies of nanostructure design, new synthetic biomaterials are appearing on the market. Such scaffolds must be tested in vitro for their biocompatibility before clinical application. However, the choice between a natural or a synthetic biomaterial might be challenging for the doctor and the patient. In this study, we compared the biocompatibility of a synthetic bone substitute, NanoBone(®) , to the widely used natural bovine bone replacement material BioOss(®) .
  
  
  METHODS
  The in vitro behaviour of human osteoblasts on both materials was investigated. Cell performance was determined using scanning electron microscopy (SEM), cell vitality staining and four biocompatibility tests (LDH, MTT, WST, BrdU).
  
  
  RESULTS
  We found that both materials showed low cytotoxicity and good biocompatibility. The MTT proliferation test was superior for Nanobone(®) .
  
  
  CONCLUSIONS
  Both scaffolds caused only little damage to human osteoblasts and justify their clinical application. However, NanoBone(®) was able to support and promote proliferation of human osteoblasts slightly better than BioOss(®) in our chosen test set-up. The results may guide doctors and patients when being challenged with the choice between a natural or a synthetic biomaterial. Further experiments are necessary to determine the comparison of biocompatibility in vivo.
authors: Dept of Oral and Maxillofacial Surgery, Kiel, Germany.
authors: Qin
authors: Q
authors: Liu
authors: Timothy
authors: T
authors: Douglas
authors: Christiane
authors: C
authors: Zamponi
authors: Stephan T
authors: ST
authors: Becker
authors: Eugene
authors: E
authors: Sherry
authors: Sureshan
authors: S
authors: Sivananthan
authors: Frauke
authors: F
authors: Warnke
authors: Jörg
authors: J
authors: Wiltfang
authors: Patrick H
authors: PH
authors: Warnke
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001857:Bone Matrix; D018786:Bone Substitutes / Q000494:pharmacology*; D001973:Bromodeoxyuridine; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D004338:Drug Combinations; D017886:Durapatite / Q000494:pharmacology*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008903:Minerals / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D012822:Silicon Dioxide / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D054457:Tissue Scaffolds
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D004396:Coloring Agents; D004338:Drug Combinations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008903:Minerals; C534915:NanoBone; D013778:Tetrazolium Salts; D013844:Thiazoles; D012822:Silicon Dioxide; D017886:Durapatite; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D001973:Bromodeoxyuridine; C018506:diacetylfluorescein