title: Immunomodulatory effect of human umbilical cord Wharton's jelly-derived mesenchymal stem cells on lymphocytes.
abstract: Studies have shown that mesenchymal stem cells (MSCs) have low immunogenicity and immune regulation. Human umbilical cord Wharton's jelly provides a new source for MSCs that are highly proliferative and have multi-differentiation potential. To investigate immunomodulatory effects of human Wharton's jelly cells (WJCs) on lymphocytes, we successfully isolated MSCs from human umbilical cord Wharton's jelly. WJCs expressed MSC markers but low levels of human leukocyte antigen (HLA)-ABC and no HLA-DR. These results indicate that WJCs have low immunogenicity. Both WJCs and their culture supernatant could inhibit the proliferation of phytohemagglutinin-stimulated human peripheral blood lymphocytes and mouse splenocytes. Additionally, WJCs suppressed secretion of transforming growth factor-β1 and interferon-γ by human peripheral blood lymphocytes. We conclude that the immunomodulatory effect of WJCs may be related to direct cell contact and inhibition of cytokine secretion by human peripheral blood lymphocytes.
authors: Bioengineering Department of Zhengzhou University, Zhengzhou, Henan Province, China.
authors: Changhui
authors: C
authors: Zhou
authors: Bo
authors: B
authors: Yang
authors: Yi
authors: Y
authors: Tian
authors: Hongliang
authors: H
authors: Jiao
authors: Wendi
authors: W
authors: Zheng
authors: Jian
authors: J
authors: Wang
authors: Fangxia
authors: F
authors: Guan
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D018655:Lymphocyte Count; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014470:Umbilical Cord / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D059631:Wharton Jelly / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D006684:HLA-DR Antigens; D010835:Phytohemagglutinins; D016212:Transforming Growth Factor beta; D007371:Interferon-gamma