title: Axonal Localization of transgene mRNA in mature PNS and CNS neurons.
abstract: Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for this in vivo. We have used a genetic approach to test for in vivo axonal transport of reporter mRNAs. We show that β-actin's 3'-UTR can drive axonal localization of GFP mRNA in mature DRG neurons, but mice with γ-actin's 3'-UTR show no axonal GFP mRNA. Peripheral axotomy triggers transport of the β-actin 3'-UTR containing transgene mRNA into axons. This GFP-3'-β-actin mRNA accumulates in injured PNS axons before activation of the transgene promoter peaks in the DRG. Spinal cord injury also increases axonal GFP signals in mice carrying this transgene without any increase in transgene expression in the DRGs. These data show for the first time that the β-actin 3'-UTR is sufficient for axonal localization in both PNS and CNS neurons in vivo.
authors: Burke-Cornell Medical Research Institute, White Plains, New York 10605, USA.
authors: Dianna E
authors: DE
authors: Willis
authors: Mei
authors: M
authors: Xu
authors: Christopher J
authors: CJ
authors: Donnelly
authors: Chhavy
authors: C
authors: Tep
authors: Marvin
authors: M
authors: Kendall
authors: Marina
authors: M
authors: Erenstheyn
authors: Arthur W
authors: AW
authors: English
authors: N Carolyn
authors: NC
authors: Schanen
authors: Catherine B
authors: CB
authors: Kirn-Safran
authors: Sung Ok
authors: SO
authors: Yoon
authors: Gary J
authors: GJ
authors: Bassell
authors: Jeffery L
authors: JL
authors: Twiss
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology*; D005786:Gene Expression Regulation / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000378:metabolism / Q000473:pathology; D013116:Spinal Cord / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D000199:Actins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D049452:Green Fluorescent Proteins; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2