title: The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice.
abstract: The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. The positional cloning of the mutation revealed a deletion in a gene encoding a protein disulphide isomerase-like enzyme (PDIL1-1). In the wild type, PDIL1-1 was expressed throughout the plant, but most intensely in the developing grain. In T3612, its expression was abolished, resulting in a decrease in the activity of plastidial phosphorylase and pullulanase, and an increase in that of soluble starch synthase I and ADP-glucose pyrophosphorylase. The amylopectin in the T3612 endosperm showed an increase in chains with a degree of polymerization 8-13 compared with the wild type. The expression in the mutant's endosperm of certain endoplasmic reticulum stress-responsive genes was noticeably elevated. PDIL1-1 appears to play an important role in starch synthesis. Its absence is associated with endoplasmic reticulum stress in the endosperm, which is likely to underlie the formation of the floury endosperm in the T3612 mutant.
authors: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
authors: Xiaohua
authors: X
authors: Han
authors: Yihua
authors: Y
authors: Wang
authors: Xi
authors: X
authors: Liu
authors: Ling
authors: L
authors: Jiang
authors: Yulong
authors: Y
authors: Ren
authors: Feng
authors: F
authors: Liu
authors: Cheng
authors: C
authors: Peng
authors: Jingjing
authors: J
authors: Li
authors: Ximing
authors: X
authors: Jin
authors: Fuqing
authors: F
authors: Wu
authors: Jiulin
authors: J
authors: Wang
authors: Xiuping
authors: X
authors: Guo
authors: Xin
authors: X
authors: Zhang
authors: Zhijun
authors: Z
authors: Cheng
authors: Jianmin
authors: J
authors: Wan
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D056625:Endosperm / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*
chemical_list: D019704:Protein Disulfide-Isomerases