title: Identification of molecular markers for DNA barcoding in the Aphidiinae (Hym. Braconidae).
abstract: Reliable identification of Aphidiinae species (Braconidae) is a prerequisite for conducting studies on aphid-parasitoid interactions at the community level. However, morphological identification of Aphidiinae species remains problematic even for specialists and is almost impossible with larval stages. Here, we compared the efficiency of two molecular markers [mitochondrial cytochrome c oxydase I (COI) and nuclear long wavelength rhodopsin (LWRh)] that could be used to accurately identify about 50 species of Aphidiinae that commonly occur in aphid-parasitoid networks in northwestern Europe. We first identified species on a morphological basis and then assessed the consistency of genetic and morphological data. Probably because of mitochondrial introgression, Aphidius ervi and A. microlophii were indistinguishable on the basis of their COI sequences, whereas LWRh sequences discriminated these species. Conversely, because of its lower variability, LWRh failed to discriminate two pairs of species (Aphidius aquilus, Aphidius salicis, Lysiphlebus confusus and Lysiphlebus fabarum). Our study showed that no unique locus but a combination of two genes should be used to accurately identify members of Aphidiinae.
authors: INRA, Agrocampus-Ouest, Université de Rennes 1, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), 65 Rue de Saint-Brieuc, CS 84215, 35042 Rennes Cedex, France. stephane.derocles@agrocampus-ouest.fr
authors: Stephane A P
authors: SA
authors: Derocles
authors: Anne
authors: A
authors: LE Ralec
authors: Manuel
authors: M
authors: Plantegenest
authors: Bernard
authors: B
authors: Chaubet
authors: Corinne
authors: C
authors: Cruaud
authors: Astrid
authors: A
authors: Cruaud
authors: Jean-Yves
authors: JY
authors: Rasplus
mesh_terms: D000818:Animals; D001042:Aphids / Q000145:classification* / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics; D005819:Genetic Markers; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D005819:Genetic Markers; D019476:Insect Proteins; D003576:Electron Transport Complex IV