title: Association of serum lipid/lipoprotein with Pro12Ala polymorphism in PPAR-γ2 among Chinese nonagenarians/centenarians.
abstract: OBJECTIVE
  In previous studies, the Pro12Ala polymorphism in the peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) was shown to be associated with both lipid metabolism and longevity. We examined whether the polymorphism continued to be associated with abnormal levels of serum lipid/lipoprotein among elderly subjects (≥90 years).
  
  
  METHODS
  The Pro12Ala variant was examined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Abnormal levels of serum lipid/lipoprotein were defined according to the criteria provided by the Chinese Medical Association (2004). Abnormal criteria were triglyceride (TG) >5.18 mmol/l, total cholesterol (TC) >1.7 mmol/l, low-density lipoprotein cholesterol (LDL-C) >3.37 mmol/l and high-density lipoprotein cholesterol (HDL-C) <1.04 mmol/l).
  
  
  RESULTS
  The sample included 673 unrelated Chinese individuals aged 90-108 years (mean age: 93.54 ± 3.54 years) and 67.3% females. Genotype frequencies of the Pro12Ala polymorphism were 0% Ala12Ala, 8.9% Pro12Ala, 91.1% Pro12Pro. Neither differences in the levels of serum lipid/lipoprotein nor the prevalence of their abnormal levels was significant between subjects who were or were not 12Ala carriers. Unadjusted and adjusted multiple logistic regressions showed that the odds ratios (OR) for abnormal levels of serum lipid/lipoprotein were not associated with the Pro12Ala polymorphism in PPAR-γ2.
  
  
  CONCLUSIONS
  Levels of serum lipid/lipoprotein were not associated with the Pro12Ala polymorphism in PPAR-γ2 among Chinese nonagenarians and centenarians, which was different from the general population.
authors: Department of Geriatrics, The Third Hospital of Mianyang, Mianyang, China. huanghj_319@yahoo.cn
authors: Huang
authors: H
authors: Chang-Quan
authors: Wang
authors: W
authors: Hui
authors: Liu
authors: L
authors: Qing-Xiu
authors: Cheng
authors: C
authors: Gang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000409:Alanine / Q000235:genetics*; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood* / Q000235:genetics; D008297:Male; D047495:PPAR gamma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011392:Proline / Q000235:genetics*
chemical_list: D017931:DNA Primers; D008055:Lipids; D008074:Lipoproteins; D047495:PPAR gamma; D011392:Proline; D000409:Alanine