title: Analysis of parameters associated with prevention of cellular apoptosis by pathogenic Neisseriae and purified porins.
abstract: The process of cellular apoptosis is mediated by a number of microbial pathogens to modulate host defense mechanisms. Inhibition of apoptosis is thought to favor microbial survival, replication or immune evasion, while induction of apoptosis is likely to promote escape of the organisms from host cells. Several studies have reported that infection with Neisseria spp. can inhibit or reduce apoptotic cell death, thus allowing adaptation, intracellular replication, and immune evasion, events that are likely to spread infection. In this chapter, various techniques are described for direct measurement of host cell responses to infection with Neisseria meningitidis and to treatment with pure Neisseria porins, the major proteins found in the outer membrane of the pathogen.
authors: Department of Medicine, Evans BioMedical Research Center, Section of Infectious Diseases, Boston University School of Medicine, Boston, MA, USA. pmassari@bu.edu
authors: Paola
authors: P
authors: Massari
authors: Lee M
authors: LM
authors: Wetzler
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005434:Flow Cytometry; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D057131:Immune Evasion / Q000502:physiology*; D007211:Indoles; D008856:Microscopy, Fluorescence; D009345:Neisseria meningitidis / Q000502:physiology*; D018272:Porins / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D014343:Trypan Blue
chemical_list: D007211:Indoles; D018272:Porins; C057699:porin protein, Neisseria; C007293:DAPI; D014343:Trypan Blue