title: GPCR production in a novel yeast strain that makes cholesterol-like sterols.
abstract: The activities of many mammalian membrane proteins including G-protein coupled receptors are cholesterol-dependent. Unlike higher eukaryotes, yeast do not make cholesterol. Rather they make a related molecule called ergosterol. As cholesterol and ergosterol are biologically non-equivalent, the potential of yeast as hosts for overproducing mammalian membrane proteins has never been fully realised. To address this problem, we are trying to engineer a novel strain of Saccharomyces cerevisiae in which the cholesterol biosynthetic pathway of mammalian cells has been fully reconstituted. Thus far, we have created a modified strain that makes cholesterol-like sterols which has an increased capacity to make G-protein coupled receptors compared to control yeast.
authors: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors: Susan M
authors: SM
authors: Kitson
authors: William
authors: W
authors: Mullen
authors: Richard J
authors: RJ
authors: Cogdell
authors: Roslyn M
authors: RM
authors: Bill
authors: Niall J
authors: NJ
authors: Fraser
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis*; D003001:Cloning, Molecular; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D030781:Organisms, Genetically Modified; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013261:Sterols / Q000096:biosynthesis / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins; D013261:Sterols; D002784:Cholesterol