title: The dominant sequence types of vancomycin-resistant Enterococcus faecium among transplantation ward patients.
abstract: OBJECTIVE
  Vancomycin-resistant enterococci (VRE) frequently cause therapeutic problems and provide information about the epidemiological condition of the ward.
  
  
  METHODS
  VRE isolated from patients on transplantation wards in 2007-2008 were compared using 2 molecular methods: RFLP-PFGE (restriction fragment length polymorphism-pulse field gel electrophoresis) and MLST (multilocus sequence typing).
  
  
  RESULTS
  The analysis covered 29 Enterococcus faecium strains resistant to glycopeptides, each from a different patient. All organisms were typed using 2 molecular methods. MLST results were compared with an international base. The 30 examined strains belonged to 8 different worldwide known sequence types. All could be recognized as representatives of a single clonal complex CC17.
  
  
  CONCLUSIONS
  Both methods of typing appeared to be useful to asses the epidemiological condition of the investigated wards.
authors: Department of Medical Microbiology, Medical University of Warsaw, Poland.
authors: A
authors: A
authors: Mlynarczyk
authors: K
authors: K
authors: Szymanek-Majchrzak
authors: E
authors: E
authors: Kosykowska
authors: W
authors: W
authors: Grzybowska
authors: S
authors: S
authors: Tyski
authors: A
authors: A
authors: Mrowka
authors: T
authors: T
authors: Baczkowska
authors: M
authors: M
authors: Durlik
authors: M
authors: M
authors: Pacholczyk
authors: A
authors: A
authors: Chmura
authors: M
authors: M
authors: Ciszek
authors: L
authors: L
authors: Paczek
authors: G
authors: G
authors: Mlynarczyk
mesh_terms: D003428:Cross Infection / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology*; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D011044:Poland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length; D019737:Transplants / Q000009:adverse effects / Q000382:microbiology*; D020713:Vancomycin Resistance