title: Two-phase olive mill waste composting: community dynamics and functional role of the resident microbiota.
abstract: In this study, physico-chemical modifications and community dynamics and functional role of the resident microbiota during composting of humid husk from a two-phase extraction system (TPOMW) were investigated. High mineralization and humification of carbon, low loss of nitrogen and complete degradation of polyphenols led to the waste biotransformation into a high-quality compost. Viable cell counts and denaturing gradient gel electrophoresis (DGGE) profiling of the 16S rRNA genes showed that the thermophilic phase was characterized by the strongest variations of cell number, the highest biodiversity and the most variable community profiles. The isolation of tannin-degrading bacteria (e.g. Lysinibacillus fusiformis, Kocuria palustris, Tetrathiobacter kashmirensis and Rhodococcus rhodochrous) suggested a role of this enzymatic activity during the process. Taken together, the results indicated that the composting process, particularly the thermophilic phase, was characterized by a rapid succession of specialized bacterial populations with key roles in the organic matter biotransformation.
authors: Department of Cellular and Environmental Biology, University of Perugia, Italy. ermanno.federici@unipg.it
authors: Ermanno
authors: E
authors: Federici
authors: Milva
authors: M
authors: Pepi
authors: Alessandro
authors: A
authors: Esposito
authors: Silvia
authors: S
authors: Scargetta
authors: Laura
authors: L
authors: Fidati
authors: Simone
authors: S
authors: Gasperini
authors: Giovanni
authors: G
authors: Cenci
authors: Roberto
authors: R
authors: Altieri
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D001711:Biotransformation; D002627:Chemistry, Physical / Q000379:methods; D004247:DNA / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D054892:Metagenome; D031658:Olea; D009930:Organic Chemicals / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods; D012987:Soil; D013634:Tannins / Q000737:chemistry
chemical_list: D007220:Industrial Waste; D009930:Organic Chemicals; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013634:Tannins; D004247:DNA