title: The expression of TSLP receptor in chronic rhinosinusitis with and without nasal polyps.
abstract: Chronic Rhinosinusitis with or without Nasal Polyps (CRSwNP and CRSsNP) may be characterized by different cytokine profiles. Generally, Th2 cytokines and eosinophilic infiltration have been reported to be more specific of CRSwNP compared to CRSsNP, where neutrophils seem to play a major role. The epithelial cell-derived thymic stromal lymphopoietin (TSLP) has been recently identified as a key factor in Th2-inflammatory response. The aim of this study is to investigate the expression of TSLP Receptor (TSLP R) in surgical specimens obtained from patients affected by CRSwNP (n=10) and CRSsNP (n=5) by immunohistochemical techniques (immunostaining score, IS). TSLP R expression was significantly higher in the inflammatory infiltrate and in the epithelial cells of CRSwNP, CRSsNP patients compared to the control group (IS 4.5±0.68, 4.4±1.44 and 0.43±0.3 respectively, p=0.0024 for inflammatory infiltrate and IS 5.8±0.92, 7.8±2.06 and 0.86±0.55 respectively, p=0.0018 for epithelial cells). No significant difference was observed in IS of inflammatory infiltrate and epithelial cells in CRSwNP compared to CRSsNP. Very low IS for TSLP R was found in connective tissue of all the samples, with no difference among the groups. TSLP receptor is highly expressed in CRS compared to controls and independently from the polyps suggesting an early common inflammatory pathway in the two CRS phenotypes.
authors: Molecular Biology Laboratory, Clinical Physiopathology Department, University of Turin, Italy.
authors: M
authors: M
authors: Boita
authors: M
authors: M
authors: Garzaro
authors: Luca
authors: L
authors: Raimondo
authors: G
authors: G
authors: Riva
authors: J
authors: J
authors: Mazibrada
authors: B
authors: B
authors: Vizio
authors: G
authors: G
authors: Bellone
authors: G
authors: G
authors: Pecorari
authors: C
authors: C
authors: Bucca
authors: G
authors: G
authors: Rolla
authors: C
authors: C
authors: Giordano
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001249:Asthma / Q000235:genetics / Q000378:metabolism; D002908:Chronic Disease; D003238:Connective Tissue / Q000378:metabolism; D004724:Endoscopy; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D010256:Paranasal Sinuses / Q000378:metabolism / Q000473:pathology; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000235:genetics*; D012220:Rhinitis / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D012852:Sinusitis / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D013256:Steroids / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; C498762:CRLF2 protein, human; D018121:Receptors, Cytokine; D013256:Steroids