title: Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures.
abstract: This review addresses methods of obtaining secondary metabolites from plant cell suspension and hairy root cultures and their exudates, particularly the physiological mechanisms of secondary metabolites release and trafficking. The efficiency for product recovery of metabolites can be increased by various methods, based on the principle of continuous product release into the cultivation medium. The most common methods for metabolite recovery are elicitation, influencing membrane permeability, and in situ product removal. The biosynthetic pathways can be influenced by cultivation conditions, transformation, or application of elicitors. The membrane permeability can be altered through the application of chemical or physical treatments. Product removal can be greatly increased through a two-phase system and the introduction of absorbents into the cultivation medium. In this review, we describe some improved approaches that have proven useful in these efforts.
authors: Institute of Food Biotechnology and Food Chemistry, Berlin University of Technology, Königin-Luise Str. 22, 14195 Berlin, Germany. zhenzhen.cai@mailbox.tu-berlin.de
authors: Zhenzhen
authors: Z
authors: Cai
authors: Anja
authors: A
authors: Kastell
authors: Dietrich
authors: D
authors: Knorr
authors: Iryna
authors: I
authors: Smetanska
mesh_terms: D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D002463:Cell Membrane Permeability; D059828:Plant Cells; D063245:Plant Development; D053147:Plant Exudates / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified
chemical_list: D053147:Plant Exudates; D010940:Plant Proteins