title: Polyamidoamine-grafted multiwalled carbon nanotubes for gene delivery: synthesis, transfection and intracellular trafficking.
abstract: Functionalized multiwalled carbon nanotubes (f-MWNTs) are of great interest and designed as a novel gene delivery system. In this paper, we presented synthesis of polyamidoamine-functionalized multiwalled carbon nanotubes (PAA-g-MWNTs) and their application as a novel gene delivery system. The PAA-g-MWNTs, obtained from amide formation between PAA and chemically oxidized MWNTs, were stable in aqueous solution and much less toxic to cells than PAA and PEI 25KDa. More importantly, PAA-g-MWNTs showed comparable or even higher transfection efficiency than PAA and PEI at optimal w/w ratio. Intracellular trafficking of Cy3-labeled pGL-3 indicated that a large number of Cy3-labeled pGL-3 were attached to nucleus membrane, the majority of which was localized in nucleus after incubation with cells for 24 h. We have demonstrated that PAA modification of MWNTs facilitate higher DNA uptake and gene expression in vitro. All these facts suggest potential application of PAA-g-MWNTs as a novel gene vector with high transfection efficiency and low cytotoxicity.
authors: Division of Nanobiomedicine, and Division of Nano-Devices and Materials, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, P. R. China.
authors: Min
authors: M
authors: Liu
authors: Biao
authors: B
authors: Chen
authors: Yanan
authors: Y
authors: Xue
authors: Jie
authors: J
authors: Huang
authors: Liming
authors: L
authors: Zhang
authors: Shiwen
authors: S
authors: Huang
authors: Qingwen
authors: Q
authors: Li
authors: Zhijun
authors: Z
authors: Zhang
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D019556:COS Cells; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D037742:Nanotubes, Carbon; C531249:Poly(amidoamine); D011073:Polyamines; D004247:DNA