title: Cytotoxic activity of extracts of marine sponges from NW Spain on a neuroblastoma cell line.
abstract: Six species of marine sponges collected at intertidal and sublittoral sites of the coast of Galicia (NW Spain) were screened for potential cytotoxic properties on Neuroblastoma BE(2)-M17 cell line. Exposure to Halichondria panicea, Pachymatisma johnstonia, Ophlitaspongia seriata and Haliclona sp. aqueous extracts strongly affected cell appearance, inducing loss of neuron-like morphology and the formation of clumps. Extracts from these species also caused significant rates of cell detachment and decrease of mitochondrial membrane potential. Incubation with P. johnstonia, O. seriata and Suberites massa extracts also decreased the rate of cell proliferation. The increase of incubation time enhanced propidium iodide uptake by neuroblastoma cells. Toxic responses triggered by sponge extracts are compatible with apoptotic phenomena in neuroblastoma cells, even though increasing propidium uptake at long periods of exposure might indicate the induction of secondary necrosis. The cytotoxic properties of the tested extracts suggest the presence of compounds with potential pharmacological or biotechnological applications in the screened sponge species.
authors: ANFACO-CECOPESCA, Area of Microbiology and Toxins, Campus Universitario 16, Vigo, 36310 Vigo (Pontevedra), Spain.
authors: Martiña
authors: M
authors: Ferreira
authors: Ana G
authors: AG
authors: Cabado
authors: María-José
authors: MJ
authors: Chapela
authors: Paula
authors: P
authors: Fajardo
authors: Miroslava
authors: M
authors: Atanassova
authors: Alejandro
authors: A
authors: Garrido
authors: Juan M
authors: JM
authors: Vieites
authors: Jorge
authors: J
authors: Lago
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D059001:Aquatic Organisms; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D011161:Porifera / Q000737:chemistry*; D011419:Propidium / Q000493:pharmacokinetics; D013030:Spain; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D014020:Tissue Extracts; D011419:Propidium