title: HPLC-CD selectivity assay for alcohol dehydrogenases.
abstract: Enantioselective reductions are a key to successful target-oriented syntheses. Finding the most suitable conditions is often a tedious work that is especially hampered by the time-consuming analytical investigation. A possible solution is the combined use of high-performance liquid chromatography and circular dichroism to find a suitable system for providing enantiomerically pure alcohols. This investigation led to an efficient protocol for the alcohol dehydrogenase-catalyzed reduction of 1-phenyl-2-propyn-3-trimethylsilyl-1-on (1).
authors: Institut für Bioorganische Chemie Heinrich-Heine-Universität Düsseldorf im Forschungszentrum, Jülich, Stetternicher Forst, Geb. 15.8, D-52426 Jülich, Germany.
authors: Melissa
authors: M
authors: Hamzic
authors: Jörg
authors: J
authors: Pietruszka
authors: Diana
authors: D
authors: Sandkuhl
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D000438:Alcohols / Q000737:chemistry; D002021:Buffers; D002384:Catalysis; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D002942:Circular Dichroism / Q000379:methods*; D008956:Models, Chemical; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
chemical_list: D000438:Alcohols; D002021:Buffers; D012997:Solvents; D000426:Alcohol Dehydrogenase