title: [Immunogenicity of sabin inactivated poliovirus vaccine induced by diphtheria-tetanus-acellular pertussis and Sabin inactivated poliovirus combined vaccine].
abstract: OBJECTIVE
  In order to search the preparation process and optimazing dosage ratio of adsorbed diphtheria-tetanus-acellular pertussis and sabin inactivated poliovirus combined vaccine (DTaP-sIPV), the neutralizing antibody titers of IPV induced by different concentration of DTaP-sIPV were investigated on rats.
  
  
  METHODS
  Two batches of DTaP-sLPV were produced using different concentration of sIPV and the quality control was carried. Together with sabin-IPV and DTaP-wIPV ( boostrix-polio, GSK, Belgium) as control group, the DTaP-sIPV were administrated on three-dose schedule at 0, 1, 2 month on rats. Serum sample were collected 30 days after each dose and neutralizing antibody titers against three types poliovirus were determined using micro-neutralization test.
  
  
  RESULTS
  Two batches of prepared DTaP-sIPV and control sLPV were according to the requirement of Chinese Pharmacopoeia (Volume III, 2005 edition) and showed good stability. The seropositivity rates were 100% for sabin inactivated poliovirus antigen in all groups. The GMTs (Geometric mean titers) of neutralizing antibodies against three types poliovirus increased.
  
  
  CONCLUSIONS
  The prepared DTaP-sIPV was safe, stable and effective and could induced high level neutralizing antibody against poliovirus on rats.
authors: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboraory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, China.
authors: Yan
authors: Y
authors: Ma
authors: Min
authors: M
authors: Qin
authors: Hui-Qiong
authors: HQ
authors: Hu
authors: Guang
authors: G
authors: Ji
authors: Ling
authors: L
authors: Feng
authors: Na
authors: N
authors: Gao
authors: Jie
authors: J
authors: Gu
authors: Bing-Feng
authors: BF
authors: Xie
authors: Ji-Hong
authors: JH
authors: He
authors: Ming-Bo
authors: MB
authors: Sun
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology*; D005260:Female; D008297:Male; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D017778:Vaccines, Combined / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D011054:Poliovirus Vaccine, Inactivated; D017778:Vaccines, Combined