title: A rapid and efficient method for isolating high quality DNA from leaves of carnivorous plants from the Drosera genus.
abstract: Drosera rotundifolia, Drosera capensis, and Drosera regia are carnivorous plants of the sundew family, characterized by the presence of stalked and sticky glands on the upper leaf surface, to attract, trap, and digest insects. These plants contain exceptionally high amounts of polysaccharides, polyphenols, and other secondary metabolites that interfere with DNA isolation and subsequent enzymatic reactions such as PCR amplification. We present here a protocol for quick isolation of Drosera DNA with high yield and a high level of purity, by combining a borate extraction buffer with a commercial DNA extraction kit, and a proteinase K treatment during extraction. The yield of genomic DNA is from 13.36 μg/g of fresh weight to 35.29 μg/g depending of the species of Drosera, with a A₂₆₀/A₂₈₀ ratio of 1.43-1.92. Moreover, the procedure is quick and can be completed in 2.5 h.
authors: UMR 1121 Nancy-Université (INPL)-INRA Agronomie et Environnement Nancy-Colmar, 2 Avenue Forêt de Haye-BP 172, 54505 Vandœuvre-lès-Nancy, France. florebiteau@yahoo.fr
authors: Flore
authors: F
authors: Biteau
authors: Estelle
authors: E
authors: Nisse
authors: Alain
authors: A
authors: Hehn
authors: Sissi
authors: S
authors: Miguel
authors: Paul
authors: P
authors: Hannewald
authors: Frédéric
authors: F
authors: Bourgaud
mesh_terms: D001881:Borates / Q000737:chemistry; D018744:DNA, Plant / Q000302:isolation & purification*; D029787:Drosera / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D018515:Plant Leaves / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D001881:Borates; D018744:DNA, Plant