title: Development of an impedimetric immunosensor for the determination of 3-amino-2-oxazolidone residue in food samples.
abstract: The use of furazolidone in food animals has been banned in European Union (EU) because of its carcinogenicity and mutagenicity on human health, but its continued misuse is widespread. Therefore, there is an urgent need for a simple, reliable, and rapid method for the detection of its marker residue, 3-amino-2-oxazolidinone (AOZ), in food products. In this regard, a sensitive and reliable electrochemical method was presented to detect AOZ based on a novel label-free electrochemical impedimetric immunosensor to address this need. The immobilization of monoclonal antibody against AOZ (denoted as AOZ-McAb) on the gold electrode was carried out through a stable acyl amino ester intermediate generated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydrosuccinimide (NHS), which could condense antibodies on the self-assembled monolayer (SAM). The detection of AOZ was performed by measuring the relative change in charge transfer resistance before and after AOZ and AOZ-McAb immunoreaction by electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the relative change in charge transfer resistance was proportional to the logarithmic value of AOZ concentrations in the range of 20.0 to 1.0×10(4) ng mL(-1) (r=0.9987). Moreover, the proposed immunosensor has a high selectivity to AOZ alone with no significant response to the metabolites of other nitrofuran antibiotics, such as 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), semicarbazide (SEM), and 1-aminohydantoin hydrochloride (AHD). This protocol has been applied to detect AOZ in food samples with satisfactory results.
authors: School of Pharmacy, China Pharmaceutical University, Nanjing, PR China. yanggongjun888@163.com
authors: Gongjun
authors: G
authors: Yang
authors: Wenjie
authors: W
authors: Jin
authors: Liping
authors: L
authors: Wu
authors: Qianqian
authors: Q
authors: Wang
authors: Hongxia
authors: H
authors: Shao
authors: Aijian
authors: A
authors: Qin
authors: Bing
authors: B
authors: Yu
authors: Dongming
authors: D
authors: Li
authors: Baoliang
authors: B
authors: Cai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D058266:Dielectric Spectroscopy; D004350:Drug Residues / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D005504:Food Analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D006827:Hydantoins / Q000737:chemistry; D007118:Immunoassay; D009025:Morpholines / Q000737:chemistry; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D012663:Semicarbazides / Q000737:chemistry
chemical_list: C504350:1-aminohydantoin; C504349:3-amino-5-morpholinomethyl-2-oxazolidinone; D000911:Antibodies, Monoclonal; D006827:Hydantoins; D009025:Morpholines; D023303:Oxazolidinones; D012663:Semicarbazides; C010059:carbamylhydrazine; D006046:Gold; C000209:3-amino-2-oxazolidinone