title: Interaction between NF-κB signaling and Notch signaling in gliogenesis of mouse mesencephalic neural crest cells.
abstract: In the present study, we elucidated that nuclear factor-κB (NF-κB) participates in the gliogenic specification of mouse mesencephalic neural crest cells. Whereas transfection of the NF-κB expression vector stimulated gliogenesis, treatment with the dominant negative NF-κB expression vector or NF-κB small interfering RNA suppressed the promotion of gliogenic specification by FGF treatment or Notch activation. This suppression was recovered by the treatment with the Deltex-1 expression vector or mammalian hairy and enhancer of split homologs expression vectors. Furthermore, transfection of the inhibitor of κB (IκB) expression vector inhibited gliogenesis. In addition, treatment with the NF-κB expression vector promoted the expression of Deltex-1. These data suggest that NF-κB signaling is implicated in the gliogenesis through the interaction with Notch signaling. Moreover, cells that contain Sox10 expressed NF-κB and Deltex-1 in the presumptive trigeminal ganglia of embryonic day 9.0-9.5 mouse embryos. This observation supports our notion that the interaction between NF-κB signaling and Notch signaling plays an important role in the gliogenic specification of mouse mesencephalic neural crest cells.
authors: Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, Japan.
authors: Kyohei
authors: K
authors: Fujita
authors: Saki
authors: S
authors: Yasui
authors: Takeshi
authors: T
authors: Shinohara
authors: Kazuo
authors: K
authors: Ito
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D008636:Mesencephalon / Q000166:cytology* / Q000196:embryology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases
chemical_list: D004268:DNA-Binding Proteins; D016328:NF-kappa B; D034741:RNA, Small Interfering; D051880:Receptors, Notch; D005346:Fibroblast Growth Factors; C104035:Dtx1 protein, mouse; D044767:Ubiquitin-Protein Ligases