title: Genetic characterization of H1N2 influenza a virus isolated from sick pigs in Southern China in 2010.
abstract: In China H3N2 and H1N1 swine influenza viruses have been circulating for many years. In January 2010, before swine were infected with foot and mouth disease in Guangdong, some pigs have shown flu-like symptoms: cough, sneeze, runny nose and fever. We collected the nasopharyngeal swab of all sick pigs as much as possible. One subtype H1N2 influenza viruses were isolated from the pig population. The complete genome of one isolate, designated A/swine/Guangdong/1/2010(H1N2), was sequenced and compared with sequences available in GenBank. The nucleotide sequences of all eight viral RNA segments were determined, and then phylogenetic analysis was performed using the neighbor-joining method. HA, NP, M and NS were shown to be closely to swine origin. PB2 and PA were close to avian origin, but NA and PB1were close to human origin. It is a result of a multiple reassortment event. In conclusion, our finding provides further evidence about the interspecies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus (SIV) surveillance, especially before the emergence of highly pathogenic FMDs in pigs in Guangdong.
authors: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors: Wei Li
authors: WL
authors: Kong
authors: Liang Zong
authors: LZ
authors: Huang
authors: Hai Tao
authors: HT
authors: Qi
authors: Nan
authors: N
authors: Cao
authors: Liang Quan
authors: LQ
authors: Zhang
authors: Heng
authors: H
authors: Wang
authors: Shang Song
authors: SS
authors: Guan
authors: Wen Bao
authors: WB
authors: Qi
authors: Pei Rong
authors: PR
authors: Jiao
authors: Ming
authors: M
authors: Liao
authors: Gui Hong
authors: GH
authors: Zhang
mesh_terms: D000818:Animals; D002681:China; D016000:Cluster Analysis; D019143:Evolution, Molecular; D016679:Genome, Viral; D005838:Genotype; D057221:Influenza A Virus, H1N2 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins