title: Analysis of CD38 and ZAP70 mRNA  expression among cytogenetic subgroups of Iranian chronic-lymphocytic-leukemia patients.
abstract: Chromosomal  abnormalities and ZAP70 expression profile are two major independent prognostic  markers in B-cell chronic lymphocytic leukemia. We investigated a possible  correlation between these two markers. ZAP70 expression using real-time RT-PCR  was examined in 20 B-cell chronic lymphocytic leukemia patients with del13q14,  13 patients with del11q22, 15 patients with trisomy 12, and 16 patients with no  detected chromosomal abnormalities. Molecular analysis revealed that ZAP70  expression in the del13q subgroup was the same as in the control group, while  it increased 2.78-fold in the del11q subgroup and 2.95-fold in the trisomy 12  subgroup, compared to the 15 cases in the control group. Comparison of the mean  and standard deviation of the ZAP70 expression profile within the subgroups  showed it to be highly variable among the individuals of the del11q and trisomy  12 subgroups, versus tight clustering for the del13q subgroup. Therefore, there  is a correlation between del13q aberration, which has good prognosis with  normal levels of ZAP70 expression. Due to a high degree of variation, no  conformity is seen for del11q and trisomy 12 subgroups, making this grouping  poor for prognostic discrimination. As a result, neither of these markers can  serve as sole discriminators to determine the course of the disease; the use of  both markers improves prognostic assessment.
authors: Cellular and Molecular Research Center, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors: H
authors: H
authors: Teimori
authors: M T
authors: MT
authors: Akbari
authors: M
authors: M
authors: Hamid
authors: M
authors: M
authors: Forouzandeh
authors: E
authors: E
authors: Bibordi
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000096:biosynthesis* / Q000235:genetics; D002872:Chromosome Deletion; D002877:Chromosomes, Human / Q000235:genetics; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007492:Iran; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D014314:Trisomy; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1