title: Ginsenoside Rg1 enhances endothelial progenitor cell angiogenic potency and prevents senescence in vitro.
abstract: This study investigated the effect of ginsenoside Rg1 on the functions of ex vivo cultivated endothelial progenitor cells (EPCs) and whether ginsenoside Rg1 prevented EPC senescence. EPCs isolated from peripheral blood from healthy volunteers were incubated with different concentrations of ginsenoside Rg1 and the effects were observed at different time points. Cell proliferation and in vitro vasculogenesis were assayed and flow cytometry was used to determine the effects of ginsenoside Rg1 on the cell cycle. Senescence and telomerase activity in EPCs were also assayed. It was found that ginsenoside Rg1 promoted EPC proliferation and vasculogenesis in dose-and time-dependent manners. Cell-cycle analysis showed that ginsenoside Rg1 increased the proliferative phase and decreased the resting phase of EPCs. β-Galactosidase and telomerase activities increased. These results support the view that ginsenoside Rg1 induces EPC proliferation and angiogenesis, and inhibits EPC senescence.
authors: Department of Cardiology, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, China.
authors: A-W
authors: AW
authors: Shi
authors: N
authors: N
authors: Gu
authors: X-M
authors: XM
authors: Liu
authors: X
authors: X
authors: Wang
authors: Y-Z
authors: YZ
authors: Peng
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D002491:Central Nervous System Agents / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects*; D019098:Telomerase; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002491:Central Nervous System Agents; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C509186:TERT protein, human; D019098:Telomerase; D001616:beta-Galactosidase; C035054:ginsenoside Rg1