title: Alteration of purinergic neurotransmission in isolated atria of streptozotocin-induced diabetic rats.
abstract: Cardiac dysfunctions are described in diabetes. However, the role of purinergic neurotransmission in diabetes-related cardiovascular diseases is unknown. The purpose of this study was to evaluate the purinergic neurotransmission in isolated atria from streptozotocin-induced diabetic rats. The animals were grouped as control and diabetic with 30 days (D30) and 60 days (D60) after streptozotocin-induced diabetes. The isolated left and right atria were used in functional experiments. The effects of adenosine triphosphate, uridine diphosphate, and adenosine were evaluated on atrial inotropism and chronotropism. The antagonists 8-cyclopentyl-1,3-dipropylxanthine and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate were also used, as blockers of P1 and P2 receptors, respectively. A negative inotropic effect followed by a positive inotropic effect was induced by adenosine triphosphate in isolated atria. This negative inotropic effect was decreased by 25% in left atria of D30. Additionally, the apparent affinity for adenosine was diminished in left atria of D30, suggesting changes in P1 receptor function. No changes were found in the right atria of D30 stimulated by adenosine. The left atria and right atria stimulated by uridine diphosphate showed an increased inotropic effect of 92% and 17%, respectively. No changes were observed in left and right atria of D30 stimulated by uridine diphosphate. Our data showed the involvement of purinergic neurotransmission in diabetes-related cardiovascular changes.
authors: Department of Pharmacology, Federal University of São Paulo, São Paulo, Brazil.
authors: Diego Castro
authors: DC
authors: Musial
authors: Kleber
authors: K
authors: de Magalhães Galvão
authors: Regiane
authors: R
authors: Miranda-Ferreira
authors: Edilson
authors: E
authors: Dantas da Silva Júnior
authors: Afonso
authors: A
authors: Caricati-Neto
authors: Neide Hyppolito
authors: NH
authors: Jurkiewicz
authors: Aron
authors: A
authors: Jurkiewicz
authors: Ana Carla
authors: AC
authors: Broetto Biazon
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D048909:Diabetes Complications / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism; D008297:Male; D058913:Purinergic Agonists / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000187:drug effects / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000378:metabolism*; D013311:Streptozocin; D013997:Time Factors; D014530:Uridine Diphosphate / Q000494:pharmacology
chemical_list: D058913:Purinergic Agonists; D018047:Receptors, Purinergic P1; D018048:Receptors, Purinergic P2; D014530:Uridine Diphosphate; D013311:Streptozocin; D000255:Adenosine Triphosphate