title: Thirteen microsatellites developed by SSR-enriched pyrosequencing for Solanum rostratum (Solanaceae) and related species.
abstract: OBJECTIVE
  Microsatellite markers were developed using second-generation sequencing in Solanum rostratum as a tool to study the reproductive biology and genetic structure of this invasive species.
  
  
  RESULTS
  Thirteen microsatellites were successfully discovered and amplified in a single multiplexed PCR. All loci showed genetic variation in S. rostratum. Cross-amplification in five closely related taxa was successful for a subset of loci.
  
  
  CONCLUSIONS
  The set of 13 microsatellite markers developed here provides a time-effective and cost-effective genetic tool to study the reproductive biology of S. rostratum. The demonstrated transferability of the PCR multiplex to related taxa also highlights its usefulness for evolutionary studies across Solanum sect. Androceras.
authors: Biological and Environmental Sciences, School of Natural Sciences, University of Stirling, United Kingdom. mario.vallejo@stir.ac.uk
authors: Mario
authors: M
authors: Vallejo-Marín
authors: Lislie
authors: L
authors: Solis-Montero
authors: Cecile F E
authors: CF
authors: Bacles
authors: Olivier
authors: O
authors: Lepais
mesh_terms: D004587:Electrophoresis, Agar Gel; D056426:Genetic Loci / Q000235:genetics; D005820:Genetic Testing; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D032322:Solanum / Q000235:genetics*; D013045:Species Specificity; D013696:Temperature