title: Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats.
abstract: Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs.
authors: Departamento de Pediatria, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: A M A
authors: AM
authors: Liberatore
authors: F C
authors: FC
authors: Moreira
authors: T A T
authors: TA
authors: Gomes
authors: J L
authors: JL
authors: Menchaca-Diaz
authors: I H J
authors: IH
authors: Koh
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000502:physiology*; D002648:Child; D054308:Enteropathogenic Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008643:Mesentery / Q000382:microbiology; D008833:Microcirculation; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000382:microbiology