title: Co-circulation of bluetongue and epizootic haemorrhagic disease viruses in cattle in Reunion Island.
abstract: Bluetongue virus (BTV) and epizootic haemorrhagic disease virus (EHDV) in deer have already been isolated in Reunion Island and have caused more or less severe clinical signs in cattle (EHDV) or in sheep (BTV), as observed in 2003. In January 2009, cattle in Reunion Island showed clinical signs suggesting infection by one or the other of these arboviral diseases. A study was set up to determine the etiology of the disease. Analysis by reverse transcriptase-polymerase chain reaction (RT-PCR) performed on blood samples from 116 cattle from different districts of the island detected the presence of the EHDV genome in 106 samples and, in 5 of them, the simultaneous occurrence of BTV and EHDV. One strain of EHDV (7 isolates) and one of BTV were isolated in embryonated eggs and a BHK-21 cell culture. Group and subgroup primer-pairs were designed on the segment 2 sequences available in GenBank to identify and type the EHDV strains. Phylogenetic analysis of the genomic segment 2 (encoding the VP2 serotype-specific protein) of the isolates confirmed the serotypes of these two orbiviruses as BTV-2 and EHDV-6 and allowed them to be compared with previously isolated strains.
authors: French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 23 Avenue du Général de Gaulle 94706 Maisons-Alfort Cedex, France. corinne.sailleau@anses.fr
authors: Corinne
authors: C
authors: Sailleau
authors: Gina
authors: G
authors: Zanella
authors: Emmanuel
authors: E
authors: Breard
authors: Cyril
authors: C
authors: Viarouge
authors: Alexandra
authors: A
authors: Desprat
authors: Damien
authors: D
authors: Vitour
authors: Micheline
authors: M
authors: Adam
authors: Laurent
authors: L
authors: Lasne
authors: Arnaud
authors: A
authors: Martrenchar
authors: Labib
authors: L
authors: Bakkali-Kassimi
authors: Laura
authors: L
authors: Costes
authors: Stéphan
authors: S
authors: Zientara
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D060085:Coinfection / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D018164:Hemorrhagic Disease Virus, Epizootic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017508:Reunion / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers