title: Ultrathin and nanostructured ZnO-based films for fluorescence biosensing applications.
abstract: The fluorescence-based sensing capability of ultrathin ZnO-SiO(2) nanoplatforms, deposited by an integrated approach of colloidal lithography and metal organic chemical vapor deposition, has been investigated upon adsorption of fluorescein-labeled albumin, used as model analyte biomolecule. The protein immobilization process after spontaneous adsorption/desorption significantly enhances the green emission of the different ZnO-based films, as evidenced by scanning confocal microscopy, corresponding to a comparable protein coverage detected by X-ray photoelectron spectroscopy. Moreover, experiments of fluorescence recovery after photobleaching evidence that the protein lateral diffusion at the biointerface is affected by the chemical and/or topographical patterning of hybrid ZnO-SiO(2) surfaces. The used approach is very promising for biomolecular detection applications of these ZnO-SiO(2) nanoplatforms, by simple sizing of the 2D vs. 3D patterning design, which in turn is accomplished by the fine tuning of the integrated colloidal lithography-chemical vapor deposition processes.
authors: Dipartimento di Scienze Chimiche, Università degli Studi di Catania and INSTM, viale Andrea Doria n. 6, 95125 Catania, Italy. csatriano@unict.it
authors: Cristina
authors: C
authors: Satriano
authors: Maria Elena
authors: ME
authors: Fragalà
authors: Yana
authors: Y
authors: Aleeva
mesh_terms: D000418:Albumins / Q000737:chemistry; D015374:Biosensing Techniques; D019793:Fluorescein / Q000737:chemistry; D005453:Fluorescence; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000418:Albumins; D008567:Membranes, Artificial; D012822:Silicon Dioxide; D015034:Zinc Oxide; D019793:Fluorescein