title: Kinetics of ultrasound-assisted lipase-catalyzed glycerolysis of olive oil in solvent-free system.
abstract: This work reports experimental kinetic data of solvent-free glycerolysis of olive oil using a commercial immobilized lipase (Novozym 435) under the influence of ultrasound irradiation. The experiments were performed in a mechanically stirred reactor under ultrasound irradiation, evaluating the effects of temperature (50-70 °C), enzyme concentration (2.5-10 wt%) and glycerol to oil molar ratio (0.8:1-3:1). Results show that ultrasound-assisted lipase-catalyzed glycerolysis might be a potential alternative route to conventional methods, as high contents of reaction products, especially monoglycerides, were achieved at mild irradiation power supply (~130 W) and temperature, in a relatively short reaction time (2h) and low enzyme content (7.5 wt%). To completeness, two simplified kinetic modeling approaches, based on the ordered-sequential bi bi mechanism and reaction stoichiometry, were employed to represent the experimental data, thus allowing a better understanding of the reaction kinetics.
authors: Department of Food Engineering, URI - Campus de Erechim, Av Sete de Setembro 1621, Erechim 99700-000, RS, Brazil.
authors: Karina G
authors: KG
authors: Fiametti
authors: Mara K
authors: MK
authors: Ustra
authors: Débora
authors: D
authors: de Oliveira
authors: Marcos L
authors: ML
authors: Corazza
authors: Agenor
authors: A
authors: Furigo
authors: J
authors: J
authors: Vladimir Oliveira
mesh_terms: D004789:Enzyme Activation / Q000528:radiation effects; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005990:Glycerol / Q000737:chemistry* / Q000528:radiation effects*; D019220:High-Energy Shock Waves; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000528:radiation effects*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D012997:Solvents; D013010:Sonication / Q000379:methods*; D013379:Substrate Specificity / Q000528:radiation effects
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; C093667:Novozyme 435; D008049:Lipase; D005990:Glycerol