title: Comparisons of [18F]-1-deoxy-1-fluoro-scyllo-inositol with [18F]-FDG for PET imaging of inflammation, breast and brain cancer xenografts in athymic mice.
abstract: BACKGROUND
  The aim of the study was to evaluate the uptake of [(18)F]-1-deoxy-1-fluoro-scyllo-inositol ([(18)F]-scyllo-inositol) in human breast cancer (BC) and glioma xenografts, as well as in inflammatory tissue, in immunocompromised mice. Studies of [(18)F]-2-fluoro-2-deoxy-d-glucose ([(18)F]-FDG) under the same conditions were also performed.
  
  
  METHODS
  Radiosynthesis of [(18)F]-scyllo-inositol was automated using a commercial synthesis module. Tumour, inflammation and normal tissue uptakes were evaluated by biodistribution studies and positron emission tomography (PET) imaging using [(18)F]-scyllo-inositol and [(18)F]-FDG in mice bearing subcutaneous MDA-MB-231, MCF-7 and MDA-MB-361 human BC xenografts, intracranial U-87 MG glioma xenografts and turpentine-induced inflammation.
  
  
  RESULTS
  The radiosynthesis of [(18)F]-scyllo-inositol was automated with good radiochemical yields (24.6%±3.3%, uncorrected for decay, 65±2 min, n=5) and high specific activities (≥195 GBq/μmol at end of synthesis). Uptake of [(18)F]-scyllo-inositol was greatest in MDA-MB-231 BC tumours and was comparable to that of [(18)F]-FDG (4.6±0.5 vs. 5.5±2.1 %ID/g, respectively; P=.40), but was marginally lower in MDA-MB-361 and MCF-7 xenografts. Uptake of [(18)F]-scyllo-inositol in inflammation was lower than [(18)F]-FDG. While uptake of [(18)F]-scyllo-inositol in intracranial U-87 MG xenografts was significantly lower than [(18)F]-FDG, the tumour-to-brain ratio was significantly higher (10.6±2.5 vs. 2.1±0.6; P=.001).
  
  
  CONCLUSIONS
  Consistent with biodistribution studies, uptake of [(18)F]-scyllo-inositol was successfully visualized by PET imaging in human BC and glioma xenografts, with lower accumulation in inflammatory tissue than [(18)F]-FDG. The tumour-to-brain ratio of [(18)F]-scyllo-inositol was also significantly higher than that of [(18)F]-FDG for visualizing intracranial glioma xenografts in NOD SCID mice, giving a better contrast.
authors: Department of Psychiatry, University of Toronto, Toronto, ON, Canada M5T 1R8.
authors: Kristin
authors: K
authors: McLarty
authors: Matthew D
authors: MD
authors: Moran
authors: Deborah A
authors: DA
authors: Scollard
authors: Conrad
authors: C
authors: Chan
authors: Nesrin
authors: N
authors: Sabha
authors: Joydeep
authors: J
authors: Mukherjee
authors: Abhijit
authors: A
authors: Guha
authors: JoAnne
authors: J
authors: McLaurin
authors: Mark
authors: M
authors: Nitz
authors: Sylvain
authors: S
authors: Houle
authors: Alan A
authors: AA
authors: Wilson
authors: Raymond M
authors: RM
authors: Reilly
authors: Neil
authors: N
authors: Vasdev
mesh_terms: D000818:Animals; D001692:Biological Transport; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D007294:Inositol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008819:Mice, Nude; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D049268:Positron-Emission Tomography / Q000379:methods*; D011854:Radiochemistry; D014183:Transplantation, Heterologous
chemical_list: C544911:(18F)-1-deoxy-1-fluoro-scyllo-inositol; D019788:Fluorodeoxyglucose F18; D007294:Inositol