title: Human amniotic glycodelin actively regulates changes in β-catenin immunoreactivity in cultured human umbilical vein endothelial cells (HUVEC).
abstract: OBJECTIVE
  Assessment of the capacity of Glycodelin A (GdA) to modulate the aggregation of cultured human umbilical vein endothelial cells.
  
  
  METHODS
  Highly purified Glycodelin A (GdA) from late first trimester amniotic fluid has been added to cultured cells and its biological activity has been observed with immunofluorescent staining of β-catenin molecules.
  
  
  RESULTS
  GdA induces translocation of β-catenin molecules promoting cell-to-cell adhesion and formation of adherents junctions through cytoskeletal reorganization.
  
  
  CONCLUSIONS
  These data provide further mechanistic insight into the specificity of cell-to-cell adhesion, thus corroborating the role of GdA in promoting angiogenesis.
authors: Department of Gynecological-Obstetric Sciences and Urological Sciences, Sapienza University of Rome, Rome, Italy. alessandro.pala@uniroma1.it
authors: Alessandro
authors: A
authors: Pala
authors: Piera
authors: P
authors: D'Elia
authors: Giuseppe
authors: G
authors: Spampinato
authors: Eugenia
authors: E
authors: Pittaluga
authors: Giuseppe
authors: G
authors: Benagiano
mesh_terms: D022005:Adherens Junctions / Q000187:drug effects / Q000378:metabolism; D000650:Amnion / Q000737:chemistry / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D000071816:Glycodelin; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D021381:Protein Transport / Q000187:drug effects; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D051176:beta Catenin