title: Inhibition of Myb-dependent gene expression by the sesquiterpene lactone mexicanin-I.
abstract: The c-myb proto-oncogene encodes a transcription factor that is highly expressed in the progenitor cells of the hematopoietic system, where it regulates the expression of genes important for lineage determination, cell proliferation and differentiation. There is strong evidence that deregulation of c-myb expression is involved in the development of human tumors, particularly of certain types of leukemia, and breast and colon cancer. The c-Myb protein is therefore an interesting therapeutic target. Here, we have investigated the potential of natural sesquiterpene lactones (STLs), a class of compounds that are active constituents of a variety of medicinal plants, to suppress Myb-dependent gene expression. We have developed a test system that allows screening of compounds for their ability to interfere with the activation of Myb target genes. Using this assay system, we have identified the STL mexicanin-I as the first cell-permeable, low-molecular-weight inhibitor of Myb-induced gene expression.
authors: Institute for Biochemistry, Westfälische-Wilhelms-Universität, Münster, Germany.
authors: T
authors: T
authors: Bujnicki
authors: C
authors: C
authors: Wilczek
authors: C
authors: C
authors: Schomburg
authors: F
authors: F
authors: Feldmann
authors: P
authors: P
authors: Schlenke
authors: C
authors: C
authors: Müller-Tidow
authors: T J
authors: TJ
authors: Schmidt
authors: K-H
authors: KH
authors: Klempnauer
mesh_terms: D000123:Acetyltransferases / Q000037:antagonists & inhibitors; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D007783:Lactones / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy; D000090063:Proto-Oncogene Mas; D020598:Proto-Oncogene Proteins c-myb / Q000037:antagonists & inhibitors*; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D007783:Lactones; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D020598:Proto-Oncogene Proteins c-myb; D012717:Sesquiterpenes; C500170:mexicanin; C002669:parthenolide; D000123:Acetyltransferases; C077509:mim-1 protein, Gallus gallus