title: Origin of neomuscularized vessels in mice exposed to chronic hypoxia.
abstract: Exposure of mice to chronic hypoxia is one of the most often used animal models to study pulmonary hypertension. Hypoxia exposure leads to vascular remodeling and muscularization of the small parenchymal vessels in the lung. Due to the anatomical differences between mice and humans, it is not possible to determine whether the remodeled vessels originate from the arterial or venous side of the vasculature. By applying antibodies against specific marker molecules expressed by arterial (ephrinB2) and venous (EphB4) endothelial cells, we could show that remodeled parenchymal vessels in hypoxia-exposed mice are mostly of arterial origin with slight venous involvement. Using these tools, it is possible to further characterize remodeled vessels in other small animal models, such as transgenic or knockout mice. Particularly useful applications would include selection of parenchymal vessels for laser microdissection studies.
authors: Ludwig Boltzmann Institute for Lung Vascular Research, Graz, Austria.
authors: Slaven
authors: S
authors: Crnkovic
authors: Andelko
authors: A
authors: Hrzenjak
authors: Leigh M
authors: LM
authors: Marsh
authors: Andrea
authors: A
authors: Olschewski
authors: Grazyna
authors: G
authors: Kwapiszewska
mesh_terms: D056151:Airway Remodeling / Q000502:physiology*; D000818:Animals; D001158:Arteries / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002908:Chronic Disease; D004195:Disease Models, Animal; D036342:Ephrins / Q000032:analysis / Q000378:metabolism*; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D000860:Hypoxia / Q000150:complications / Q000473:pathology; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D008297:Male; D051379:Mice; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000473:pathology*; D014680:Veins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D036342:Ephrins