title: Fusion of human umbilical cord mesenchymal stem cells with esophageal             carcinoma cells inhibits the tumorigenicity of esophageal carcinoma cells.
abstract: Prior studies on the biology and therapeutic application of human stem cells             in human malignancies have reported mixed results. Some evidence shows the use             of stem cell transplantation is an important tool in the treatment of several             hematologic and non-hematologic malignancies while some others suggest both human             stem cells and mature stromal cells can contribute to the development and growth             of human malignancies. Aiming to provide more evidence on this controversial issue,             we investigated the effect of cell fusion of mesenchymal stem cells with esophageal             carcinoma cells on tumorigenesis. Results suggest that artificial fusion of human             umbilical cord mesenchymal stem cells with esophageal carcinoma cells resulted             in hybrids with declined cell growth, increased apoptosis and suppressed tumorigenicity.             The comparison of gene expression profiles of human mesenchymal stem cells, esophageal             carcinoma cells and hybrids indicated that fusion induced activation of apoptosis.             Furthermore, the expression of DUSP6/MKP3 in MAPK pathway increased strikingly             and the exogenous overexpression confirmed the growth suppression. Our results             demonstrate fusion of human mesenchymal stem cells with esophageal carcinoma cells             induced apoptosis and benign transdifferentiation rather than reprogramming to             cancer stem cells.
authors: Department of Etiology and Carcinogenesis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China.
authors: Yong
authors: Y
authors: Wang
authors: Hongbin
authors: H
authors: Fan
authors: Bing
authors: B
authors: Zhou
authors: Zhenyu
authors: Z
authors: Ju
authors: Long
authors: L
authors: Yu
authors: Liping
authors: L
authors: Guo
authors: Jingdong
authors: J
authors: Han
authors: Shixin
authors: S
authors: Lu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis; D002277:Carcinoma / Q000473:pathology*; D002454:Cell Differentiation; D002459:Cell Fusion; D045744:Cell Line, Tumor; D002470:Cell Survival; D054642:Dual Specificity Phosphatase 6 / Q000378:metabolism; D000071063:Endoglin; D004938:Esophageal Neoplasms / Q000473:pathology*; D020869:Gene Expression Profiling; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism / Q000637:transplantation*; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011956:Receptors, Cell Surface / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism; D047368:Tumor Burden; D014470:Umbilical Cord / Q000473:pathology*
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; C517035:DUSP6 protein, human; D054642:Dual Specificity Phosphatase 6