title: Gamma camera and optical imaging with a fusion reporter gene using human sodium/iodide symporter and monomeric red fluorescent protein in mouse model.
abstract: OBJECTIVE
  Multimodality imaging contributes to the activation of translational research by compensating for its weak points. Herein, we developed a noninvasive dual-reporter gene system for nuclear and optical imaging.
  
  
  METHODS
  We constructed a fusion reporter vector concurrently expressing the human sodium/iodide symporter (hNIS) and monomeric red fluorescent protein (mCherry), and evaluated the function of this fusion reporter system under in vitro and in vivo conditions.
  
  
  RESULTS
  The expression of hNIS/mCherry fusion gene was confirmed in transfected cells using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. As the numbers of cells increased, the fluorescence and 125I uptake increased in the hNIS/mCherry-transfected cells, and a high correlation between fluorescence intensity and radioactivity was noted. The fluorescence intensities and radioactivity signals were also well-correlated in HT-29-hNIS/mCherry tumors (R2=0.9304) in in vivo fluorescence and gamma camera imaging.
  
  
  CONCLUSIONS
  The dual-reporter imaging method using hNIS and mCherry genes reflected tumor extent as well as viable cell numbers, and correlated well with one another. This suggests that the hNIS/mCherry dual-reporter system can be a useful tool for multi-modal imaging.
authors: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
authors: Kwang Il
authors: KI
authors: Kim
authors: Jae Jun
authors: JJ
authors: Park
authors: Yong Jin
authors: YJ
authors: Lee
authors: Tae Sup
authors: TS
authors: Lee
authors: Kwang Sun
authors: KS
authors: Woo
authors: Inho
authors: I
authors: Song
authors: Kyeong Min
authors: KM
authors: Kim
authors: Chang Woon
authors: CW
authors: Choi
authors: Sang Moo
authors: SM
authors: Lim
authors: Joo Hyun
authors: JH
authors: Kang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D015902:Gamma Cameras; D050939:Gene Fusion / Q000235:genetics; D017930:Genes, Reporter; D019073:HT29 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D000097573:Red Fluorescent Protein; D000099209:Sodium-Iodide Symporters
chemical_list: D008164:Luminescent Proteins; D027981:Symporters; D000097573:Red Fluorescent Protein; D000099209:Sodium-Iodide Symporters