title: Purification, biochemical properties and antithrombotic effect of a novel Streptomyces enzyme on carrageenan-induced mice tail thrombosis model.
abstract: BACKGROUND
  The prevalence of cardiovascular diseases, one of the major causes of worldwide mortality, is being increasingly reported. Safer, more effective, and less expensive thrombolytic drugs can possibly overcome the underlying problems associate with current thrombolytic drugs.
  
  
  METHODS
  A thrombolytic enzyme was purified and characterized from a Streptomyces strain. Carrageenan induced tail-thrombosis mice model was used to evaluate in vivo antithrombotic effect of the enzyme.
  
  
  RESULTS
  First 15N-terminal amino acids of the purified enzyme were IAGGQAIYAGGGRRS, which are significantly different from the reported fibrinolytic enzymes. The enzyme exhibited 14.3±2.3-fold stronger thrombolytic activity than that of plasmin. In carrageenan induced tail-thrombosis model, the enzyme caused reduction in frequency of thrombus. Tail-thrombus of the enzyme treated group was significantly shorter than the physiological saline treated group and the thrombus decrement was correlated with the enzyme dose.
  
  
  CONCLUSIONS
  The enzyme purified from the Streptomyces strain can be a potential candidate for the treatment of thrombosis.
authors: Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 501–759, South Korea.
authors: Jaya Ram
authors: JR
authors: Simkhada
authors: Seung Sik
authors: SS
authors: Cho
authors: Poonam
authors: P
authors: Mander
authors: Yun Hee
authors: YH
authors: Choi
authors: Jin Cheol
authors: JC
authors: Yoo
mesh_terms: D000818:Animals; D002351:Carrageenan; D004195:Disease Models, Animal; D004789:Enzyme Activation; D004795:Enzyme Stability; D057487:Enzyme Therapy; D004798:Enzymes / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D013302:Streptomyces / Q000201:enzymology*; D013623:Tail / Q000098:blood supply / Q000187:drug effects; D016896:Treatment Outcome; D020246:Venous Thrombosis / Q000175:diagnosis / Q000188:drug therapy* / Q000503:physiopathology*
chemical_list: D004798:Enzymes; D005343:Fibrinolytic Agents; D002351:Carrageenan