title: Single nucleotide polymorphism -799C/T in matrix metalloproteinase-8 promoter region in arterial disease.
abstract: Arterial disease is associated with elevated serum matrix metalloproteinase (MMP)-8 concentration. We studied the role of two promoter region single nucleotide polymorphisms (SNPs) of MMP-8 gene in the arterial disease. The population comprised patients with arterial disease (n = 124) and healthy blood donors (n = 100) as a reference group for MMP-8 SNPs (-799C/T and -381A/G) genotypes and serum concentrations. Genotype frequencies for MMP-8 -799C/T SNP in arterial disease were C/C (43.5%), C/T (32.3%) and T/T (24.2%), and in the reference group they were C/C (50.0%), C/T (40.0%) and T/T (10.0%; P = 0.012). The -799C allele frequency was lower in the patients (59.7%) than in the reference group (70.0%; P = 0.023). The -799C allele showed protective effects against the arterial disease with an odds ratio [95% confidence interval (CI)] of 0.372 (0.141-0.980, P = 0.045) after adjustment for age, gender, and serum MMP-8 and TIMP-1 concentrations. Only in the reference group and whole study population (n = 224), the -799TT genotype significantly associated with an increase in serum MMP-8 concentrations (P = 0.047, 0.025). The -799C allele appeared protective against the arterial disease. The genotype may have an effect on systemic MMP-8 levels which could not, however, be seen in the arterial disease patients probably as a result of the strong inflammation involved in the disease pathogenesis.
authors: Institute of Dentistry, University of Helsinki, and Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Helsinki, Finland. pratikshya.pradhan-palikhe@helsinki.fi
authors: Pratikshya
authors: P
authors: Pradhan-Palikhe
authors: Pirkko J
authors: PJ
authors: Pussinen
authors: Pirkka
authors: P
authors: Vikatmaa
authors: Anil
authors: A
authors: Palikhe
authors: Anne S
authors: AS
authors: Kivimäki
authors: Mauri
authors: M
authors: Lepäntalo
authors: Tuula
authors: T
authors: Salo
authors: Timo
authors: T
authors: Sorsa
mesh_terms: D000368:Aged; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics* / Q000276:immunology; D005260:Female; D005387:Finland; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D020784:Matrix Metalloproteinase 8