title: Global genome transcription profiling of Bifidobacterium bifidum PRL2010 under in vitro conditions and identification of reference genes for quantitative real-time PCR.
abstract: Bifidobacteria have attracted significant scientific attention due to their perceived role as health-promoting microorganisms, although the genetics of the bacterial group is still underexplored. In this study, we investigated the transcriptome of Bifidobacterium bifidum PRL2010 during in vitro growth by microarray technology. When B. bifidum PRL2010 was grown in liquid broth, 425 of the 1,644 PRL2010 genes represented on the array were expressed in at least one of the three investigated growth phases, i.e., the lag, exponential, and stationary phases. These transcriptional analyses identified a core in vitro transcriptome encompassing 150 genes that are expressed in all phases. A proportion of these genes were further investigated as potential reference genes by quantitative real-time reverse transcription-PCR (qRT-PCR) assays. Their expression stability was evaluated under different growth conditions, which included cultivation on different carbon sources, exposure to environmental stresses (thermal, acidic, and osmotic), and growth phases. Our analyses validated six reference genes suitable for normalizing mRNA expression levels in qRT-PCR experiments applied to bifidobacteria.
authors: Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, Parma, Italy.
authors: Francesca
authors: F
authors: Turroni
authors: Elena
authors: E
authors: Foroni
authors: Barbara
authors: B
authors: Montanini
authors: Alice
authors: A
authors: Viappiani
authors: Francesco
authors: F
authors: Strati
authors: Sabrina
authors: S
authors: Duranti
authors: Alberto
authors: A
authors: Ferrarini
authors: Massimo
authors: M
authors: Delledonne
authors: Douwe
authors: D
authors: van Sinderen
authors: Marco
authors: M
authors: Ventura
mesh_terms: D001644:Bifidobacterium / Q000235:genetics*; D020869:Gene Expression Profiling / Q000592:standards*; D046228:Microarray Analysis / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D014158:Transcription, Genetic; D059467:Transcriptome