title: Timing of ganciclovir administration in glioma gene therapy using HSVtk gene-transduced mesenchymal stem cells.
abstract: BACKGROUND
  An established C6 glioma was successfully treated with intratumoral injection of mesenchymal stem cells transduced with HSVtk gene (MSCtk) and systemic administration of ganciclovir (GCV). The best timing of GCV administration after the MSCtk implantation was studied.
  
  
  METHODS
  GCV administration was started from 2 days before and 1, 3 and 7 days after the MSCtk administration under both in vitro and in vivo conditions.
  
  
  RESULTS
  The C6 cells were completely eradicated in vitro when GCV administration was started from day -2, 1, and 3. Animals with intracranial tumor survived longer when GCV was administered earlier after MSCtk administration. This may, mainly, reflect the difference in the MSCtk/C6 ratio at the time of GCV administration because this ratio drastically decreases during the delay of GCV administration.
  
  
  CONCLUSIONS
  When using a slowly growing vector cell as MSCtk, GCV should be administered soon after MSCtk implantation.
authors: Department of Neurosurgery, Hamamatsu University School of Medicine, Handayama, Higashi-ku, Hamamatsu, Japan.
authors: Shinji
authors: S
authors: Amano
authors: Chunyu
authors: C
authors: Gu
authors: Shinichiro
authors: S
authors: Koizumi
authors: Tsutomu
authors: T
authors: Tokuyama
authors: Hiroki
authors: H
authors: Namba
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000628:therapy*; D024201:Bystander Effect; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015774:Ganciclovir / Q000008:administration & dosage*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D005910:Glioma / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D018139:Simplexvirus / Q000235:genetics; D016019:Survival Analysis; D013937:Thymidine Kinase / Q000235:genetics*; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir