title: Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions.
abstract: Direct incorporation of azide groups into RNA oligonucleotides by in vitro transcription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are described. This postsynthetic labeling protocol is robust and modular, and offers an alternative access to RNA labeled with biophysical probes.
authors: Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, Pune 411021, India.
authors: Harita
authors: H
authors: Rao
authors: Anupam A
authors: AA
authors: Sawant
authors: Arun A
authors: AA
authors: Tanpure
authors: Seergazhi G
authors: SG
authors: Srivatsan
mesh_terms: D001386:Azides / Q000737:chemistry*; D001483:Base Sequence; D057930:Click Chemistry / Q000379:methods; D009843:Oligoribonucleotides / Q000737:chemistry*; D014544:Uridine Triphosphate / Q000031:analogs & derivatives*
chemical_list: D001386:Azides; D009843:Oligoribonucleotides; D014544:Uridine Triphosphate