title: The antibacterial lectin RegIIIgamma promotes the spatial segregation of microbiota and host in the intestine.
abstract: The mammalian intestine is home to ~100 trillion bacteria that perform important metabolic functions for their hosts. The proximity of vast numbers of bacteria to host intestinal tissues raises the question of how symbiotic host-bacterial relationships are maintained without eliciting potentially harmful immune responses. Here, we show that RegIIIγ, a secreted antibacterial lectin, is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface. Loss of host-bacterial segregation in RegIIIγ(-/-) mice was coupled to increased bacterial colonization of the intestinal epithelial surface and enhanced activation of intestinal adaptive immune responses by the microbiota. Together, our findings reveal that RegIIIγ is a fundamental immune mechanism that promotes host-bacterial mutualism by regulating the spatial relationships between microbiota and host.
authors: Department of Immunology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
authors: Shipra
authors: S
authors: Vaishnava
authors: Miwako
authors: M
authors: Yamamoto
authors: Kari M
authors: KM
authors: Severson
authors: Kelly A
authors: KA
authors: Ruhn
authors: Xiaofei
authors: X
authors: Yu
authors: Omry
authors: O
authors: Koren
authors: Ruth
authors: R
authors: Ley
authors: Edward K
authors: EK
authors: Wakeland
authors: Lora V
authors: LV
authors: Hooper
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D058491:Bacterial Load; D006090:Gram-Negative Bacteria / Q000276:immunology / Q000502:physiology*; D006094:Gram-Positive Bacteria / Q000276:immunology / Q000502:physiology*; D006706:Homeostasis; D007070:Immunoglobulin A / Q000032:analysis; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology*; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology*; D037181:Lectins, C-Type / Q000502:physiology; D054892:Metagenome; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins / Q000378:metabolism*; D013559:Symbiosis; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D007070:Immunoglobulin A; D037181:Lectins, C-Type; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D000073718:Pancreatitis-Associated Proteins; D011506:Proteins; C490255:Reg3g protein, mouse