title: Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan.
abstract: A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle (36/72, 50%) and pigs (101/157, 64.3%) whereas groups A (44/89, 49.4%) and D (40/89, 44.9%) were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance (minimum inhibitory concentrations: ≥32 μg/ml) was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates (nine from poultry and two from cattle) produced extended spectrum β-lactamase (ESBL). The two bovine isolates produced bla(CTX-M-2), while the nine poultry isolates produced bla(CTX-M-25) (4), bla(SHV-2) (3), bla(CTX-M-15) (1) and bla(CTX-M-2) (1). Thus, our results showed that several types of ESBL were identified and three types of β-lactamase (SHV-2, CTX-M-25 and CTX-M-15) were observed for the first time in E. coli from diseased animals in Japan.
authors: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511. asai-t@nval.maff.go.jp
authors: Tetsuo
authors: T
authors: Asai
authors: Kaori
authors: K
authors: Masani
authors: Chizuru
authors: C
authors: Sato
authors: Mototaka
authors: M
authors: Hiki
authors: Masaru
authors: M
authors: Usui
authors: Kotaro
authors: K
authors: Baba
authors: Manao
authors: M
authors: Ozawa
authors: Kazuki
authors: K
authors: Harada
authors: Hiroshi
authors: H
authors: Aoki
authors: Takuo
authors: T
authors: Sawada
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology; D018550:Cephalosporin Resistance / Q000235:genetics*; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D020387:Food Chain; D005516:Food Microbiology; D007564:Japan / Q000453:epidemiology; D008460:Meat / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology; D013552:Swine / Q000382:microbiology; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; D001618:beta-Lactamases