title: [Determination of three chemical constituents in zhuang medicine Blumea megacephala (Randeria) by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application.
  
  
  METHODS
  The determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution. The flow rate was 1 mL min(-1), and the detected wavelength was set at 258, 327nm.
  
  
  RESULTS
  The peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg x L(-1) for protocatechuic acid, 15.6-156 mg x L(-1) for chlorogenic acid, 3.96-39.6 mg x L(-1) for caffeic acid. The average recoveries were 103.4%, 102.2%, 98.5%, respectively.
  
  
  CONCLUSIONS
  The method was proved to be simple, accurate and used for the quality evaluation of Blumea megacephala (Randeria).
authors: Guangxi College of Traditional Chinese Medicine, Nanning 530001, China. lb30654@qq.com
authors: Xiaoqing
authors: X
authors: Ning
authors: Yaohua
authors: Y
authors: Li
authors: Yuanfeng
authors: Y
authors: Tan
authors: Yong
authors: Y
authors: Chen
authors: Yan
authors: Y
authors: Huang
authors: Fuqi
authors: F
authors: Pan
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002109:Caffeic Acids / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D062385:Hydroxybenzoates / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C009091:protocatechuic acid; C040048:caffeic acid