title: RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil.
abstract: Twenty-three Bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from Brazilian soil samples were analyzed for the presence and genetic diversity (by RE-PCR) of the virulence genes ces (emetic toxin, cereulide), plcR-papR (pleiotropic regulator PlcR and peptide PapR), nheA (a component of the NHE complex), bceT (diarrheal enterotoxin bc-D-ENT), gyrB (B subunit of DNA gyrase), cytK-2 (necrotic enterotoxin cytotoxin K-2), and plcA (phosphatidylcholine-specific phospholipase C). Additionally, these isolates were phenotypically characterized for motility, hemolytic and lecithinase activities, as well as HBL enterotoxin production. The group of isolates associated with food poisoning had the highest occurrence of the phenotypically analyzed factors and the most frequent occurrence and highest genetic diversity of the plcR-papR, nheA, bceT, cytK-2, plcA, and gyrB genes. An analysis of molecular variance (AMOVA), in which all loci were analyzed, demonstrated that the genetic variation intragroup of isolates (92%) was significantly higher than that intergroup (8%) (P<0.05). These results were corroborated by an analysis of the genetic differentiation between the groups, which was low/moderate, the result of a high degree of allele sharing. Our results suggest that B. cereus isolates with the potential to cause food poisoning outbreaks do not have a specific genetic profile characterized by the presence of a particular gene or allele among the genes assessed. On the contrary, different combinations of genes encoding virulence factors may be present in different isolates of B. cereus that potentially cause food poisoning outbreaks.
authors: Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina, CP 6001, 86051-990 Londrina/PR, Brazil.
authors: C A
authors: CA
authors: Santos
authors: F S
authors: FS
authors: Almeida
authors: A G
authors: AG
authors: Guimarães
authors: W M
authors: WM
authors: Abrahão
authors: O M N
authors: OM
authors: Arantes
authors: G T
authors: GT
authors: Vilas-Bôas
mesh_terms: D001409:Bacillus cereus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001938:Brazil; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000032:analysis / Q000235:genetics; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004768:Enterotoxins; D037521:Virulence Factors; D004262:DNA Restriction Enzymes