title: Distribution of immune cells and expression of interleukin  receptors in ileal Peyer's patches of calves.
abstract: Newborn calves lack a mature immune system. The immune system develops with age, but the role of the expression of cytokine receptors in the development of immune cells of Peyer's patches (PPs) in the intestines of calves in the first 2 months has not yet been elucidated. In this study, the distribution of immune cells and the expression of interleukin (IL) receptors (R) in the ileal PPs of newborn and 2-month-old calves were investigated immunohistochemically with monoclonal antibodies against bovine CD4, CD8, IgM, γδTCR, T19, WC3, WC5, and WC6 antigens. The expression of ILRs was examined with antibodies against CD25 (IL-2Rα), IL-2Rγ, IL-4R, IL-6R, IL-10R, and IL-13R antigens. CD4(+), CD8(+), γδTCR(+), T19(+), and WC6(+) cells were found to be more widely distributed in the ileal PPs of 2-month-old calves than in those of newborn calves. Moreover, the expression of CD25 (IL-2Rα), IL-4R, and IL-13R in the ileal PPs of 2-month-old calves was more prominent than that in newborn calves. These data suggest that the immune system of calves at 2 months of age is developed by reactions to foreign antigens and aging.
authors: Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors: Hidehisa
authors: H
authors: Tamao
authors: Yasuo
authors: Y
authors: Inoshima
authors: Naotaka
authors: N
authors: Ishiguro
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D007082:Ileum / Q000166:cytology* / Q000378:metabolism*; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D010581:Peyer's Patches / Q000166:cytology* / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000276:immunology / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D018123:Receptors, Interleukin