title: Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves.
abstract: Phosphorus (P) is a structural component of nucleic acids and phospholipids and plays important roles in plant growth and development. P accumulation was significantly reduced (about 35%) in rice leaves from plants grown under low (32 μM) P compared to 320 μM P grown plants. Genome response to low P was examined using the rice 60K oligonucleotide DNA microarrays. At the threshold significance of |log₂| fold>2.0, 21,033 genes (about 33.7% of all genes on the microarray) were affected by P deficiency. Among all genes on the microarray, 4271 genes were sorted into 51 metabolic pathways. Low P affected 1494 (35.0%) genes and the largest category of genes was related to sucrose degradation to ethanol and lactate pathway. To survey the role of P in rice, 25 pathways were selected based on number of affected genes. Among these pathways, cytosolic glycolysis contained the least number of upregulated but most down-regulated genes. Low P decreased glucose, pyruvate and chlorophyll, and genes related to carbon metabolism and chlorophyllide a biosynthesis. However, sucrose and starch levels increased. These results indicate that P nutrition affects diverse metabolic pathways mostly related to glucose, pyruvate, sucrose, starch, and chlorophyll a.
authors: Institute of Agricultural Science & Technology, Chonbuk National University, Jeonju, Jeollabuk-do 561-756, Republic of Korea.
authors: Myoung Ryoul
authors: MR
authors: Park
authors: So-Hyeon
authors: SH
authors: Baek
authors: Benildo G
authors: BG
authors: de Los Reyes
authors: Song Joong
authors: SJ
authors: Yun
authors: Karl H
authors: KH
authors: Hasenstein
mesh_terms: D050260:Carbohydrate Metabolism / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005947:Glucose / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010710:Phosphates / Q000172:deficiency* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D013395:Sucrose / Q000378:metabolism
chemical_list: D010710:Phosphates; D010937:Plant Growth Regulators; D002734:Chlorophyll; D013395:Sucrose; D019289:Pyruvic Acid; D013213:Starch; D005947:Glucose; D000077194:Chlorophyll A