title: [Effect of ultrafiltration by different membranes on HPLC fingerprint of huanglian jiedu decorction after being].
abstract: OBJECTIVE
  To investigate the change of HPLC fingerprint of Huanglian Jiedu decroction. Huanglian Jiedu decorction was ultrafiltrated by different membranes.
  
  
  METHODS
  Different relative molecular weight cutoff membranes were used (pes-5w, pes-3w, pes-2w, pes-1w, pes-6k) to explore its effects on content differences among all samples in HPLC. The turbidity, viscosity and other physical and chemical parameters were measured.
  
  
  RESULTS
  Berberine, baicalein and jatrorrhizine became smaller while geniposide increased. Physicall and chemical parameters also showed regular changes. Taking centrifugate as reference, the similarities of the samples treated with high to low molecular weight cutoff membranes were 0.993, 0.992, 0.984, 0.982, 0.975, respectively.
  
  
  CONCLUSIONS
  In this study, the ultrafiltration was used, HPLC fingerprint showed the differences of the samples, and the change of the physical and chemical parameters was explored.
authors: Nanjing University of Chinese Medicine, Key Laboratory of Separation Engineering for Chinese Medicine Compound, Nanjing 210029, China. xumingyubernadat@163.com
authors: Mingyu
authors: M
authors: Xu
authors: Hongliang
authors: H
authors: Zhou
authors: Liwei
authors: L
authors: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C061676:oren gedoku to