title: The effect of indapamide on development of myocardial hypertrophy and fibrosis in L-NAME-induced hypertension in rat.
abstract: The aim of this study was to analyze the effect of indapamide and its combination with ACE inhibitor (captopril) and antioxidant (Provinols™) on both myocardial hypertrophy and fibrosis. Wistar rats were treated with L-NAME (40 mg/kg/day, L); L-NAME plus indapamide (1 mg/kg/day), or captopril (10 mg/kg/day), or Provinols™ (40 mg/kg/day), or combination of indapamide with captopril, and indapamide with Provinols™ for 7 weeks. Blood pressure (BP), LV hypertrophy and fibrosis were determined. The content of collagens type I and III was evaluated morphometrically after picrosirius red staining. L-NAME treatment led to increased BP, LV hypertrophy, total fibrosis and relative content of collagens without the change in collagen type I/III ratio. Indapamide and captopril decreased BP, LV hypertrophy and the collagen ratio without affecting total fibrosis, while Provinols™ reduced BP, the collagen ratio and fibrosis without affecting LV hypertrophy. The combinations decreased all the parameters. Decrease of LV hypertrophy was achieved by drugs with the best reducing effect on BP, fibrosis reduction was reached by the antioxidant treatment with only partial effect on BP. Thus, the combination of antihypertensive and antioxidant treatment may represent a powerful tool in preventing myocardial remodeling induced by hypertension.
authors: Department of Pathology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic.
authors: L
authors: L
authors: Hlavačková
authors: S
authors: S
authors: Vranková
authors: P
authors: P
authors: Janega
authors: O
authors: O
authors: Pecháňová
authors: P
authors: P
authors: Babál
mesh_terms: D000818:Animals; D002216:Captopril / Q000494:pharmacology; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005355:Fibrosis / Q000139:chemically induced* / Q000378:metabolism; D006973:Hypertension / Q000139:chemically induced* / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism*; D007190:Indapamide / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000473:pathology*; D019331:NG-Nitroarginine Methyl Ester / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D002216:Captopril; D007190:Indapamide; D019331:NG-Nitroarginine Methyl Ester