title: Sexually dimorphic gene expression in non-human primate ESCs analyzed stringently.
abstract: Human exhibit sexual dimorphism early in development and throughout life. Here we stringently analyzed gene expression in inbred non-human primate embryonic stem cells (nhpESCs) searching for sexually dimorphisms. We utilized location-specific probes solely, thus avoiding probe cross-reactivity between members of gene families and genomic gene duplications. Seventeen sexually dimorphic transcripts (15 genes, out of which 9 autosomals) were identified, of which five were verified using real-time q-PCR. We compared these results from pedigreed nhpESCs with available human ESCs datasets. Three human X-linked genes show sexual dimorphism. Thus, these results enhance our knowledge and deepen our understanding on early development processes for sexual dimorphism.
authors: Bioinformatics Program, School of Computer Science, The Academic College of Tel Aviv-Yaffo, Tel Aviv, Israel. dorits@mta.ac.il
authors: Dorit
authors: D
authors: Shweiki
authors: Ahmi
authors: A
authors: Ben-Yehudah
authors: Carrie
authors: C
authors: Redinger
authors: Charles A
authors: CA
authors: Easley
authors: Shany
authors: S
authors: Doron
authors: Gerald
authors: G
authors: Schatten
mesh_terms: D000818:Animals; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011323:Primates; D012727:Sex Characteristics; D014158:Transcription, Genetic