title: Computational analysis of RNA-protein interaction interfaces via the Voronoi diagram.
abstract: Cellular functions are mediated by various biological processes including biomolecular interactions, such as protein-protein, DNA-protein and RNA-protein interactions in which RNA-Protein interactions are indispensable for many biological processes like cell development and viral replication. Unlike the protein-protein and protein-DNA interactions, accurate mechanisms and structures of the RNA-Protein complexes are not fully understood. A large amount of theoretical evidence have shown during the past several years that computational geometry is the first pace in understanding the binding profiles and plays a key role in the study of intricate biological structures, interactions and complexes. In this paper, RNA-Protein interaction interface surface is computed via the weighted Voronoi diagram of atoms. Using two filter operations provides a natural definition for interface atoms as classic methods. Unbounded parts of Voronoi facets that are far from the complex are trimmed using modified convex hull of atom centers. This algorithm is implemented to a database with different RNA-Protein complexes extracted from Protein Data Bank (PDB). Afterward, the features of interfaces have been computed and compared with classic method. The results show high correlation coefficients between interface size in the Voronoi model and the classical model based on solvent accessibility, as well as high accuracy and precision in comparison to classical model.
authors: Laboratory of Systems Biology and Bioinformatics, Institute of Biochemistry and Biophysics and COE in Biomathematics, University of Tehran, Tehran, Iran.
authors: Sedigheh
authors: S
authors: Mahdavi
authors: Ali
authors: A
authors: Mohades
authors: Ali
authors: A
authors: Salehzadeh Yazdi
authors: Samad
authors: S
authors: Jahandideh
authors: Ali
authors: A
authors: Masoudi-Nejad
mesh_terms: D000465:Algorithms; D000818:Animals; D030562:Databases, Protein; D008957:Models, Genetic; D008958:Models, Molecular; D011485:Protein Binding / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods; D012313:RNA / Q000378:metabolism*
chemical_list: D012313:RNA