title: Effects of botulinum toxin a on cytokine synthesis in a cell culture model of cutaneous scarring.
abstract: OBJECTIVE
  To evaluate possible botulinum toxin A effects in a cell culture model.
  
  
  METHODS
  In a cell culture model with dermal fibroblasts and microvascular endothelial cells, possible botulinum toxin A effects were evaluated. Cell proliferation and cytokine expression were analyzed using viability assays and enzyme-linked immunosorbent assay techniques.
  
  
  RESULTS
  Neither cell proliferation nor cytokines and growth factors (interleukin 6, monocyte chemoattractant protein 2, fibroblast growth factor, macrophage colony-stimulating factor, and vascular endothelial growth factor) were affected by botulinum toxin A incubation.
  
  
  CONCLUSIONS
  The present data do not add evidence to suggest a significant therapeutic role of botulinum toxin A injections for cutaneous wound healing beyond chemoimmobilization. Further studies that include patient-specific cells of hypertrophic scars are required to better understand what role botulinum toxin A can play in the treatment of mature scar tissue.
authors: Laboratory of Facial Plastic Surgery Research, Department of Otorhinolaryngology, University of Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, Germany. frank.haubner@klinik.uni-regensburg.de
authors: Frank
authors: F
authors: Haubner
authors: Elisabeth
authors: E
authors: Ohmann
authors: Uwe
authors: U
authors: Müller-Vogt
authors: Peter
authors: P
authors: Kummer
authors: Juergen
authors: J
authors: Strutz
authors: Holger G
authors: HG
authors: Gassner
authors: Holger
authors: H
authors: Gassner
mesh_terms: D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D002921:Cicatrix / Q000188:drug therapy / Q000378:metabolism; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D012680:Sensitivity and Specificity; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D016207:Cytokines; D042461:Vascular Endothelial Growth Factor A; D019274:Botulinum Toxins, Type A