title: The proteomic research of the cure of experimental diabetes deafness by granules of eliminating phlegm and removing blood stasis.
abstract: OBJECTIVE
  Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis ((see text) also known as GEPRB), screening outand identifying the differentially expressed proteins by mass spectrometry and bioinformatics analysis, discussing the molecular mechanism of control the Diabetes deafness by GEPRB.
  
  
  METHODS
  By use of proteomics technology, the serum protein serum proteome of the control group, model control group, Duxil and each observation group were observed for 2-DE gel pattern matching, and the difference in the relative content of 2 times was chosen for the differentially expressed proteins. Identification of differentially expressed proteins by MALDI-TOF MS/MS, the authors further analysis the phosphorylation, subcellular localization, interaction, direct regulation, and transmembrane of the differences proteins by the way of bioinformatics analysis. Sixty SPF level SD rats elected in diabetic rats model group (abbreviated as DM group) were be randomly divided into 5 groups based on random number sheet, namely model control group, positive drug control group (Du-ke-xi group) and Mai-tong-fang high, medium and low dose group respectively. In addition, set of normal control group. 10 rats in each group.
  
  
  RESULTS
  By Coomassie brilliant blue staining, identified 51 differential protein spots dug from 2-D gel by mass spectrometry, successfully identified 13 non-redundant proteins. Most of the identified proteins were secreted protein and belong to different protein families. There were about 12 proteins have the transmembrane region from the authors' result, ten of them were plasma membrane proteins.
  
  
  CONCLUSIONS
  It's suggesting that 13 differential proteins is most likely the protein response to GEPRB in vivo, these proteins may play key role for the treatment of GEPRB to Diabetes deafness. The two highly differentially expressed proteins Apolipoprotein E (apoE) and C3 may be a potential drug target of GEPRB.
authors: Chengdu University of Traditinoal Chinese Medicine, Chengdu, Sichuan 610075, China. guo256@yahoo.cn
authors: Hong
authors: H
authors: Guo
authors: Da-Jing
authors: DJ
authors: Xiong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D003638:Deafness / Q000188:drug therapy*; D048909:Diabetes Complications / Q000188:drug therapy*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013311:Streptozocin
chemical_list: D001798:Blood Proteins; D013311:Streptozocin