title: Methylation status of CpG sites in the MCP-1 promoter is correlated to serum MCP-1 in Type 2 diabetes.
abstract: OBJECTIVE
  Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine and plays an important role in atherosclerosis of Type 2 diabetes. The aim of this study was to investigate the methylation status of CpG sites in the MCP-1 promoter in Type 2 diabetic patients and its correlation to serum MCP- 1 level, and blood glucose level.
  
  
  METHODS
  The 32 patients with Type 2 diabetes and 15 healthy controls were enrolled into the study. Bodymass index, blood pressure, blood lipid, blood glucose, glycosylated hemoglobin (HbA1c), and serum MCP-1 were measured. Genomic DNA was isolated fromthe peripheral blood mononuclear cells (PBMC). Methylation status of CpG sites in theMCP-1 promoter was determined using methylation specific polymerase chain reaction.
  
  
  RESULTS
  The promoter region (2890-3050 bp) was predominantly methylated in PBMC from controls.Methylation of CpGmotifs were less methylated in the patients than in the controls (25% vs 80%; p<0.001), while the level of MCP-1 in serum was higher in patients with Type 2 diabetes (193.95±74.96 vs 88.46±55.10; p<0.001). MCP-1 promoter methylation was significantly correlated to serum MCP-1, HbA1c, fasting blood glucose, and triglyceride.
  
  
  CONCLUSIONS
  These data suggest that hypomethylation of CpG sites in the MCP-1 promoter region may be affected by blood glucose and TG, which then increase the serum MCP-1 level and may play a role in the vascular complications of Type 2 diabetes.
authors: Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Avenue Jiefang 1277#, Wuhan, Hubei, PR China.
authors: Z H
authors: ZH
authors: Liu
authors: L L
authors: LL
authors: Chen
authors: X L
authors: XL
authors: Deng
authors: H J
authors: HJ
authors: Song
authors: Y F
authors: YF
authors: Liao
authors: T S
authors: TS
authors: Zeng
authors: J
authors: J
authors: Zheng
authors: H Q
authors: HQ
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000209:etiology* / Q000473:pathology; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood* / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D048909:Diabetes Complications / Q000209:etiology / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D018932:Chemokine CCL2; D006442:Glycated Hemoglobin; D014280:Triglycerides; C111835:CCL2 protein, human