title: Dimethylformamide is not better than glycerol for cryopreservation of boar semen.
abstract: To improve the boar sperm cryopreservation process, the influence of the sugar (lactose, trehalose) source and the cryoprotectant [glycerol, dimethylformamide (DMF)] on the success of freezing was investigated. Sperm samples were frozen in one of six extenders: lactose plus 3% glycerol (LG); lactose plus 1.5% glycerol and 1.5% DMF (LGD); lactose plus 3% DMF (LD); trehalose plus 3% glycerol (TG); trehalose plus 1.5% glycerol and 1.5% DMF (TGD); trehalose plus 3% DMF (TD). Effects on motility, viability, acrosome integrity and hypoosmotic test (HOST) were measured. The results showed that extender containing 3% glycerol retained the highest motility percentages. In regard to viability and acrosome integrity, all extenders yielded similar rates except for the decreasing values of TD. Endosmosis was diminished in TD and LD at 2 h (P = 0.0018), as compared with the others. The results of the study demonstrated that the use of DMF as a cryoprotectant adversely affected boar sperm quality after cryopreservation.
authors: Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary Medicine, University of Zaragoza, Zaragoza, Spain. 485010@celes.unizar.es
authors: C
authors: C
authors: Malo
authors: L
authors: L
authors: Gil
authors: R
authors: R
authors: Cano
authors: F
authors: F
authors: Martínez
authors: A
authors: A
authors: García
authors: R A
authors: RA
authors: Jerez
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004126:Dimethylformamide / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D008297:Male; D012662:Semen Preservation; D013552:Swine
chemical_list: D004126:Dimethylformamide; D005990:Glycerol