title: Antibody-dependent enhancement of Marburg virus infection.
abstract: BACKGROUND
  Marburg virus (MARV) and Ebola virus (EBOV) cause severe hemorrhagic fever in primates. Earlier studies demonstrated that antibodies to particular epitopes on the glycoprotein (GP) of EBOV enhanced virus infectivity in vitro.
  
  
  METHODS
  To investigate this antibody-dependent enhancement (ADE) in MARV infection, we produced mouse antisera and monoclonal antibodies (mAbs) to the GPs of MARV strains Angola and Musoke.
  
  
  RESULTS
  The infectivity of vesicular stomatitis virus pseudotyped with Angola GP in K562 cells was significantly enhanced in the presence of Angola GP antisera, whereas only minimal ADE activity was seen with Musoke GP antisera. This difference correlated with the percentage of hybridoma clones producing infectivity-enhancing mAbs. Using mAbs to MARV GP, we identified 3 distinct ADE epitopes in the mucinlike region on Angola GP. Interestingly, some of these antibodies bound to both Angola and Musoke GPs but showed significantly higher ADE activity for strain Angola. ADE activity depended on epitopes in the mucinlike region and glycine at amino acid position 547, present in the Angola but absent in the Musoke GP.
  
  
  CONCLUSIONS
  These results suggest a possible link between ADE and MARV pathogenicity and provide new insights into the mechanisms underlying ADE entry of filoviruses.
authors: Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.
authors: Eri
authors: E
authors: Nakayama
authors: Daisuke
authors: D
authors: Tomabechi
authors: Keita
authors: K
authors: Matsuno
authors: Noriko
authors: N
authors: Kishida
authors: Reiko
authors: R
authors: Yoshida
authors: Heinz
authors: H
authors: Feldmann
authors: Ayato
authors: A
authors: Takada
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D019067:Antibody-Dependent Enhancement; D015922:Complement C1q / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005260:Female; D017353:Gene Deletion; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D020014:K562 Cells; D008379:Marburg Virus Disease / Q000276:immunology* / Q000821:virology; D029024:Marburgvirus / Q000276:immunology* / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D014764:Viral Proteins; D015922:Complement C1q