title: Mimosine-induced apoptosis in C6 glioma cells requires the release of mitochondria-derived reactive oxygen species and p38, JNK activation.
abstract: Growth-inhibitory effects of mimosine, a plant amino acid, on rat C6 glioma cells were analyzed. Mimosine markedly inhibited proliferation and induced apoptosis of C6 glioma cells in a dose- and time-dependent manner. Mimosine-mediated apoptosis was accompanied by promoting reactive oxygen species (ROS) generation in mitochondria, and by decreased mitochondrial membrane potential (Δψ), and release of cytochrome c from mitochondria, followed by caspase 3 activation. Furthermore, mimosine increased the phosphorylation level of c-Jun-N-terminal protein kinase and p38, which was the downstream effect of ROS accumulation. Mimosine was confirmed to show profound effects on apoptosis of C6 glioma cells by ROS-regulated mitochondria pathway, and these results bear on the hypothesized potential for mimosine as promising agents in the treatment of malignant gliomas.
authors: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Aichi, 487-8501, Japan. qiaosl@isc.chubu.ac.jp
authors: Shanlou
authors: S
authors: Qiao
authors: Keiko
authors: K
authors: Murakami
authors: Qinghong
authors: Q
authors: Zhao
authors: Baoling
authors: B
authors: Wang
authors: Hisao
authors: H
authors: Seo
authors: Hitoshi
authors: H
authors: Yamashita
authors: Xiaotao
authors: X
authors: Li
authors: Takashi
authors: T
authors: Iwamoto
authors: Masatoshi
authors: M
authors: Ichihara
authors: Masataka
authors: M
authors: Yoshino
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D020552:Comet Assay; D045304:Cytochromes c / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D008898:Mimosine / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D008898:Mimosine; D045304:Cytochromes c; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D053148:Caspase 3; D053179:Caspase 7