title: Phenylboronic acid is a more potent inhibitor than boric acid of key signaling networks involved in cancer cell migration.
abstract: Previous studies from our lab have shown that both boric (BA) and phenylboronic- acid (PBA) inhibit the migration of prostate cancer cell lines, as well as non-tumorigenic prostate cells. Our results indicate that PBA is more potent than BA in targeting metastatic and proliferative properties of cancer cells. Here we focus on the impact of BA and PBA on Rho family of GTP-binding proteins and their downstream targets. Treatment with 1mM PBA and BA decreases activities of RhoA, Rac1, and Cdc42 in DU-145 metastatic prostate cancer cells, but not in normal RWPE-1 prostate cells. Furthermore, ROCKII activity and phosphorylation of myosin light chain kinase decrease as a result of either PBA or BA treatment in DU-145 cells, suggesting these compounds target actomyosin-based contractility.
authors: Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY USA.
authors: Erin M
authors: EM
authors: McAuley
authors: Tiffany A
authors: TA
authors: Bradke
authors: George E
authors: GE
authors: Plopper
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism; D001888:Boric Acids / Q000008:administration & dosage*; D001897:Boronic Acids / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D008297:Male; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011471:Prostatic Neoplasms / Q000473:pathology; D015398:Signal Transduction / Q000187:drug effects*; D020764:cdc42 GTP-Binding Protein / Q000187:drug effects / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000187:drug effects / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism
chemical_list: D000199:Actins; D001888:Boric Acids; D001897:Boronic Acids; D054460:rho-Associated Kinases; D009219:Myosin-Light-Chain Kinase; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020741:rho GTP-Binding Proteins; C010686:benzeneboronic acid; C032688:boric acid