title: Maternal prenatal anxiety and downregulation of placental 11β-HSD2.
abstract: BACKGROUND
  Raised maternal anxiety during pregnancy is associated with increased risk of adverse neurodevelopmental outcomes for her child. The mechanisms underlying this are not known but animal studies suggest prenatal stress may alter the function of the placenta. Here we determined whether maternal prenatal anxiety was associated with a downregulation of placental 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2), the enzyme which metabolises cortisol.
  
  
  METHODS
  We recruited mothers the day before delivery by elective caesarean, and gave them the Spielberger Trait and State anxiety and Edinburgh Depression self-rating scales. Placentae were collected and aliquots stored for later analysis.
  
  
  RESULTS
  Prenatal Trait anxiety was negatively correlated with placental 11β-HSD2 mRNA expression (r=-0.40, p<0.01, n=56). Results were similar with male and female fetuses (r=-0.39, p=0.04, n=28; r=-0.40, p=0.03, n=28) respectively. Results were also significant with State anxiety (r=-0.27, p=0.05, n=56) but somewhat weaker for depression (r=-0.20, p=0.13, n=56). Preliminary analyses on a subset of cases (n=25) suggested parallel results for enzyme activity.
  
  
  CONCLUSIONS
  These findings provide evidence for an association between prenatal maternal mood and downregulation of placental 11β-HSD2. Results are consistent with raised maternal anxiety being associated with increased fetal exposure to maternal cortisol, and support the hypothesis that this may be one mechanism underlying fetal programming by prenatal stress.
authors: Institute of Reproductive and Developmental Biology, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK. Kieran.odonnell@mail.mcgill.ca
authors: Kieran J
authors: KJ
authors: O'Donnell
authors: Anna
authors: A
authors: Bugge Jensen
authors: Laura
authors: L
authors: Freeman
authors: Natasha
authors: N
authors: Khalife
authors: Thomas G
authors: TG
authors: O'Connor
authors: Vivette
authors: V
authors: Glover
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000378:metabolism*; D000328:Adult; D000339:Affect / Q000502:physiology; D001007:Anxiety / Q000378:metabolism* / Q000523:psychology*; D002585:Cesarean Section / Q000523:psychology; D003863:Depression / Q000523:psychology; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006801:Humans; D008297:Male; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy / Q000378:metabolism* / Q000523:psychology*; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012959:Socioeconomic Factors; D011795:Surveys and Questionnaires
chemical_list: D012313:RNA; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2