title: Human oocytes reprogram somatic cells to a pluripotent state.
abstract: The exchange of the oocyte's genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cells affected in degenerative human diseases. Such cells, carrying the patient's genome, might be useful for cell replacement. Here we report that the development of human oocytes after genome exchange arrests at late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, the resultant triploid cells develop to the blastocyst stage. Stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies removal of the oocyte genome as the primary cause of developmental failure after genome exchange.
authors: The New York Stem Cell Foundation Laboratory, New York, New York, USA.
authors: Scott
authors: S
authors: Noggle
authors: Ho-Lim
authors: HL
authors: Fung
authors: Athurva
authors: A
authors: Gore
authors: Hector
authors: H
authors: Martinez
authors: Kathleen Crumm
authors: KC
authors: Satriani
authors: Robert
authors: R
authors: Prosser
authors: Kiboong
authors: K
authors: Oum
authors: Daniel
authors: D
authors: Paull
authors: Sarah
authors: S
authors: Druckenmiller
authors: Matthew
authors: M
authors: Freeby
authors: Ellen
authors: E
authors: Greenberg
authors: Kun
authors: K
authors: Zhang
authors: Robin
authors: R
authors: Goland
authors: Mark V
authors: MV
authors: Sauer
authors: Rudolph L
authors: RL
authors: Leibel
authors: Dieter
authors: D
authors: Egli
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015894:Genome, Human / Q000235:genetics; D005855:Germ Layers / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D018587:Oocyte Donation; D009865:Oocytes / Q000166:cytology* / Q000254:growth & development / Q000502:physiology*; D061251:Primary Cell Culture; D014158:Transcription, Genetic; D057885:Triploidy; D055815:Young Adult