title: EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis.
abstract: Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.
authors: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors: Hong
authors: H
authors: Cao
authors: Xiaoqian
authors: X
authors: Leng
authors: Chuangju
authors: C
authors: Li
authors: Qiwei
authors: Q
authors: Wei
authors: Jianfang
authors: J
authors: Gui
authors: Hanhua
authors: H
authors: Cheng
authors: Rongjia
authors: R
authors: Zhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D018076:DNA, Complementary / Q000235:genetics; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D020869:Gene Expression Profiling; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D010902:Pituitary Gland / Q000378:metabolism*; D010907:Pituitary Hormones / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C064448:somatolactin protein, fish