title: Glutathione transferase pi (GSTpi) expression in breast cancer: an immunohistochemical and molecular study.
abstract: Breast cancer is the most frequent cancer in women worldwide. Prognostic markers are important for diagnosis, allowing therapeutic strategies to be defined more efficiently. The expression of the glutathione S-transferase pi isoenzyme (GSTpi) in tumor cells has been evaluated as a predictor of prognosis and in response to cytotoxic treatments. Its immunoexpression was assessed in 63 women diagnosed with invasive ductal carcinoma in a retrospective study. The results were statistically correlated with clinicopathological parameters of patients. The results showed that high GSTpi expression was related to p53-positive tumors, grade III histology, large tumor size and death (p<0.05). The 37 patients who received adjuvant treatment, checked separately, showed high expression of GSTpi in relation to local recurrence, metastasis and death (p<0.05). In addition, high levels of GSTpi expression were significantly associated with a shorter overall survival (p<0.05). To confirm this suspicion, GSTpi gene expression was checked by Real-time PCR in neoplastic mammary cells cultured and subjected to treatment with doxorubicin. Our results suggest that high levels of GSTpi may be related to the development of resistance to chemotherapy in these tumors, the response of these tumors to treatment and the clinical course of the patients involved.
authors: Graduate Program in Genetics, Universidade Estadual Paulista, IBILCE, São José do Rio Preto, SP, Brazil.
authors: Bruna Victorasso
authors: BV
authors: Jardim
authors: Marina Gobbe
authors: MG
authors: Moschetta
authors: Gabriela Bottaro
authors: GB
authors: Gelaleti
authors: Camila
authors: C
authors: Leonel
authors: Vitor Rafael
authors: VR
authors: Regiani
authors: Dalisio
authors: D
authors: de Santi Neto
authors: Newton A
authors: NA
authors: Bordin-Junior
authors: Silvia Aparecida
authors: SA
authors: Perea
authors: Debora Ap Pires de Campos
authors: DA
authors: Zuccari
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D051549:Glutathione S-Transferase pi