title: Comparison of humoral immune responses to Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray.
abstract: BACKGROUND
  Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).
  
  
  METHODS
  To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.
  
  
  RESULTS
  In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.
  
  
  CONCLUSIONS
  The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.
authors: Viral Oncology Program, Sidney Kimmel Cancer Center, Baltimore, Maryland, USA.
authors: Dasheng
authors: D
authors: Zheng
authors: Jun
authors: J
authors: Wan
authors: Yong Gu
authors: YG
authors: Cho
authors: Leyao
authors: L
authors: Wang
authors: Chuang-Jiun
authors: CJ
authors: Chiou
authors: Shweta
authors: S
authors: Pai
authors: Crystal
authors: C
authors: Woodard
authors: Jian
authors: J
authors: Zhu
authors: Gangling
authors: G
authors: Liao
authors: Otoniel
authors: O
authors: Martinez-Maza
authors: Jiang
authors: J
authors: Qian
authors: Heng
authors: H
authors: Zhu
authors: Gary S
authors: GS
authors: Hayward
authors: Richard F
authors: RF
authors: Ambinder
authors: S Diane
authors: SD
authors: Hayward
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D018023:HIV Seronegativity / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D007070:Immunoglobulin A / Q000276:immunology; D016483:Lymphoma, AIDS-Related / Q000097:blood / Q000276:immunology / Q000821:virology; D016393:Lymphoma, B-Cell / Q000097:blood / Q000276:immunology / Q000821:virology; D040081:Protein Array Analysis / Q000379:methods*; D012514:Sarcoma, Kaposi / Q000097:blood / Q000276:immunology / Q000821:virology; D019562:Viral Load / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D007070:Immunoglobulin A