title: Identification of major dioxin-like compounds and androgen receptor antagonist in acid-treated tissue extracts of high trophic-level animals.
abstract: We evaluated the applicability of combining in vitro bioassays with instrument analyses to identify potential endocrine disrupting pollutants in sulfuric acid-treated extracts of liver and/or blubber of high trophic-level animals. Dioxin-like and androgen receptor (AR) antagonistic activities were observed in Baikal seals, common cormorants, raccoon dogs, and finless porpoises by using a panel of rat and human cell-based chemical-activated luciferase gene expression (CALUX) reporter gene bioassays. On the other hand, no activity was detected in estrogen receptor α (ERα)-, glucocorticoid receptor (GR)-, progesterone receptor (PR)-, and peroxisome proliferator-activated receptor γ2 (PPARγ2)-CALUX assays with the sample amount applied. All individual samples (n = 66) showed dioxin-like activity, with values ranging from 21 to 5500 pg CALUX-2,3,7,8-tetrachlorodibenzo-p-dioxin equivalent (TEQ)/g-lipid. Because dioxins are expected to be strong contributors to CALUX-TEQs, the median theoretical contribution of dioxins calculated from the result of chemical analysis to the experimental CALUX-TEQs was estimated to explain up to 130% for all the tested samples (n = 54). Baikal seal extracts (n = 31), but not other extracts, induced AR antagonistic activities that were 8-150 μg CALUX-flutamide equivalent (FluEQ)/g-lipid. p,p'-DDE was identified as an important causative compound for the activity, and its median theoretical contribution to the experimental CALUX-FluEQs was 59% for the tested Baikal seal tissues (n = 25). Our results demonstrate that combining in vitro CALUX assays with instrument analysis is useful for identifying persistent organic pollutant-like compounds in the tissue of wild animals on the basis of in vitro endocrine disruption toxicity.
authors: Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies, Tsukuba, Japan. g-suzuki@nies.go.jp
authors: Go
authors: G
authors: Suzuki
authors: Nguyen M
authors: NM
authors: Tue
authors: Sander
authors: S
authors: van der Linden
authors: Abraham
authors: A
authors: Brouwer
authors: Bart
authors: B
authors: van der Burg
authors: Martin
authors: M
authors: van Velzen
authors: Marja
authors: M
authors: Lamoree
authors: Masayuki
authors: M
authors: Someya
authors: Shin
authors: S
authors: Takahashi
authors: Tomohiko
authors: T
authors: Isobe
authors: Yuko
authors: Y
authors: Tajima
authors: Tadasu K
authors: TK
authors: Yamada
authors: Hidetaka
authors: H
authors: Takigami
authors: Shinsuke
authors: S
authors: Tanabe
mesh_terms: D059002:Androgen Receptor Antagonists / Q000378:metabolism*; D000818:Animals; D004147:Dioxins / Q000378:metabolism*; D006801:Humans; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D019576:Porpoises; D046031:Raccoon Dogs; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D046048:Seals, Earless; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D059002:Androgen Receptor Antagonists; D004147:Dioxins; D047492:Peroxisome Proliferator-Activated Receptors; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011965:Receptors, Glucocorticoid; D014874:Water Pollutants, Chemical