title: Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrodes modified by multi-walled carbon nanotubes.
abstract: A glassy carbon electrode (GC) was modified by multi-walled carbon nanotubes (MWCNTs). The modified electrode showed a pair of redox peaks that resulted from the oxygen-containing functional groups on the nanotube surface. A recombinant thermostable dye-linked L-proline dehydrogenase (L-proDH) from hyperthermophilic archaeon (Thermococcus profundus) was further immobilized by physical adsorption. The modified electrode (GC/MWCNTs/L-proDH) exhibited an electrocatalytic signal for L-proline compared to bare GC, GC/L-proDH and GC/MWCNTs electrodes, which suggested that the presence of MWCNTs efficiently enhances electron transfer between the active site of enzyme and electrode surface. The immobilized L-proDH showed a typical Michaelis-Menten catalytic response with lower apparent constant.
authors: School of Environmental Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, P.R. China.
authors: Haitao
authors: H
authors: Zheng
authors: Leyi
authors: L
authors: Lin
authors: Yosuke
authors: Y
authors: Okezaki
authors: Ryushi
authors: R
authors: Kawakami
authors: Haruhiko
authors: H
authors: Sakuraba
authors: Toshihisa
authors: T
authors: Ohshima
authors: Keiichi
authors: K
authors: Takagi
authors: Shin-Ichiro
authors: S
authors: Suye