title: The N-acetyl-D-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes.
abstract: To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl-D-glucosamine-utilization genes that are co-regulated during productive symbiosis. We also demonstrate that repression by NagC is relieved in the presence of N-acetyl-D-glucosamine-6-phosphate, the intracellular form of N-acetyl-D-glucosamine. We find that gene repression by NagC is critical for efficient colonization of E. scolopes. Further, our study shows that NagC regulates genes that affect the normal dynamics of host colonization.
authors: Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706-1521, USA. miyashiro@wisc.edu
authors: Tim
authors: T
authors: Miyashiro
authors: Will
authors: W
authors: Klein
authors: Dane
authors: D
authors: Oehlert
authors: Xiaodan
authors: X
authors: Cao
authors: Julia
authors: J
authors: Schwartzman
authors: Edward G
authors: EG
authors: Ruby
mesh_terms: D000117:Acetylglucosamine / Q000096:biosynthesis*; D048908:Aliivibrio fischeri / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D058491:Bacterial Load; D049832:Decapodiformes / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins; D037521:Virulence Factors; D000117:Acetylglucosamine