title: [Preparation and identification of monoclonal antibody against hMOF].
abstract: OBJECTIVE
  To prepare and identify the monoclonal antibody (mAb) against hMOF.
  
  
  METHODS
  BALB/C mice were immunized with protein from the spleen cells isolated and fused with SP2/0 cells. After several rounds of screening and cloning, the hybridoma cell strain secreting anti-hMOF mAb was obtained. Its specificity was evaluated with ELISA and Western blot, and the titer, immunoglobulin subtype and affinity of the mAb were measured.
  
  
  RESULTS
  One cell strains of hybridoma were obtained and named as 4C1C8. The anti-hMOF mAb secreted by the hybridoma cell strain was identified as IgG1 subtype. The mAb titers in ascitic fluid were 1 409600, as determined with ELISA with an affinity reaching to 7.65 x 10(6) L/mol. Western blot demonstrated that the antibodies could specifically recognize the immunogen. The cell immunohistochemistry proved that the antibody could recognize the hMOF antigens expressed on the normal cells HL7702.
  
  
  CONCLUSIONS
  The success in anti-hMOF mAb preparation provides the basis for further study of hMOF.
authors: Department of Immuniology, Chongqing Three Gorges Medicine Colloge, Wanzhou 404016, China.
authors: Hou-Liang
authors: HL
authors: Sun
authors: Da-Peng
authors: DP
authors: Wei
authors: Xiao-Hong
authors: XH
authors: Zhang
authors: Yan-Jun
authors: YJ
authors: Liu
authors: Chong-Jie
authors: CJ
authors: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D051548:Histone Acetyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins
chemical_list: D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D051548:Histone Acetyltransferases; C496552:KAT8 protein, human