title: Anti-inﬂammatory effects of active constituents extracted from Chinese medicinal herbs against Propionibacterium acnes.
abstract: Interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-α) from keratinocyte play important roles in initiating the inflammatory process of acne. They are used as major elements to evaluate the anti-inflammatory activity of drugs. In this study, various active constituents extracted from Chinese medicinal herbs were tested for their anti-inflammatory effects against P. acnes using ELISA. Among the constituents, matrine, baicalin, ursolic acid, sodium danshensu, magnolol, honokiol, hesperidin and andrographolide significantly reduced IL-8 and TNF-α by human HaCaT keratinocyte cells pretreated with heat-killed P. acnes. Excepting hesperidin, these active constituents presented dose-dependent inhibitory effects. Our studies showed that all of them exhibited low cytotoxicity at 5 µg mL⁻¹ in tested cell lines, and even at 50 µg mL⁻¹, in the cases of matrine, baicalin, ursolic acid and sodium danshensu. Based on the obtained results, it can be suggested that these active constituents are potential acne-mitigating candidates for cosmetic applications.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Shiyu
authors: S
authors: Fu
authors: Changlei
authors: C
authors: Sun
authors: Xinyi
authors: X
authors: Tao
authors: Yuhong
authors: Y
authors: Ren
mesh_terms: D000893:Anti-Inflammatory Agents / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000382:microbiology; D017705:Lignans / Q000737:chemistry / Q000494:pharmacology; D011425:Propionibacterium acnes / Q000187:drug effects*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology; D000097245:Ursolic Acid; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016209:Interleukin-8; D017705:Lignans; D014315:Triterpenes; D000097245:Ursolic Acid; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C038044:baicalin