title: [The role of vimentin during PRRSV infection of Marc-145 cells].
abstract: The objective of this study was to investigate the function of vimentin in PRRSV infection. Vimentin gene from Marc-145 cells was amplified by RT-PCR, cloned into pET-28a vector and expressed in Escherichia coli BL21(DE3). The expressed vimentin was confirmed by Western blot and purified which was used to immunize BALB/c mice for the production of antibodies. Vimentin and antibodies were tested for blocking PRRSV infection of Marc-145 cells. The binding of vimentin to PRRSV N and GP5 proteins were tested by the ELISA. The results showed that vimentin gene was amplified successfully and expressed as identified by SDS-PAGE and Western blot. Mouse anti-vimentin antibodies were produced with the titer of 10(5). PRRSV infection of Marc-145 cells was blocked partially by vimentin while blocked completely by the antibobies. In addition, vimentin was bound N protein, but not GP5. These results provide additional information on PRRSV entry into Marc-145 cells.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, TaiAn, Shandong 271018, China. wangww@163.com
authors: Wei-Wei
authors: WW
authors: Wang
authors: Lu
authors: L
authors: Zhang
authors: Xiao-Chun
authors: XC
authors: Ma
authors: Ji-Ming
authors: JM
authors: Gao
authors: Yi-Hong
authors: YH
authors: Xiao
authors: En-Min
authors: EM
authors: Zhou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D011485:Protein Binding / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013552:Swine; D014746:Vimentin / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D014746:Vimentin; D014764:Viral Proteins