title: Complete genomic sequence of bacteriophage felix o1.
abstract: Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.
authors: Mailstop G29, Centers for Disease Control and Prevention; 1600 Clifton Road, Atlanta, GA 30329, USA.
authors: Jean M
authors: JM
authors: Whichard
authors: Smithsonian National Institution, National Museum in Natural History, MRC 534, Washington, DC 20560, USA.
authors: Lee A
authors: LA
authors: Weigt
authors: Abbot Point of Care, 185 Corkstown Road, Ottawa, ON, K2H 8V4, Canada.
authors: Douglas J
authors: DJ
authors: Borris
authors: Pennsylvania State University, Department of Veterinary and Biomedical Sciences, 204 Wartick Laboratory, University Park, PA 16802 , USA.
authors: Ling Ling
authors: LL
authors: Li
authors: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. Seattle, WA 98109, USA.
authors: Qing
authors: Q
authors: Zhang
authors: Pennsylvania State University, 115 Henning Bldg., University Park, PA 16802, USA.
authors: Vivek
authors: V
authors: Kapur
authors: VA-MD Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Duck Pond Drive (0442), Blacksburg, Virginia 24061, USA.
authors: F William
authors: FW
authors: Pierson
authors: Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario N1G 3W4, Canada.
authors: Erika J
authors: EJ
authors: Lingohr
authors: Centre for Biologics Research, Health Canada, Room D159, Frederick G. Banting Building 251 Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, ON K1A 0K9, Canada.
authors: Yi-Min
authors: YM
authors: She
authors: Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, Ontario N1G 3W4, Canada.|University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario N1G 2W1, Canada.
authors: Andrew M
authors: AM
authors: Kropinski
authors: Center for Molecular Medicine and Infectious Disease; 1410 Prices Fork Road; Blacksburg, VA 24061-0342, USA.
authors: Nammalwar
authors: N
authors: Sriranganathan