title: Utilization of Triton X-100 and polyethylene glycols during surfactant-mediated biodegradation of diesel fuel.
abstract: The hypothesis regarding preferential biodegradation of surfactants applied for enhancement of microbial hydrocarbons degradation was studied. At first the microbial degradation of sole Triton X-100 by soil isolated hydrocarbon degrading bacterial consortium was confirmed under both full and limited aeration with nitrate as an electron acceptor. Triton X-100 (600 mg/l) was utilized twice as fast for aerobic conditions (t(1/2)=10.3h), compared to anaerobic conditions (t(1/2)=21.8h). HPLC/ESI-MS analysis revealed the preferential biodegradation trends in both components classes of commercial Triton X-100 (alkylphenol ethoxylates) as well as polyethylene glycols. The obtained results suggest that the observed changes in the degree of ethoxylation for polyethylene glycol homologues occurred as a consequence of the 'central fission' mechanism during Triton X-100 biodegradation. Subsequent experiments with Triton X-100 at approx. CMC concentration (150 mg/l) and diesel oil supported our initial hypothesis that the surfactant would become the preferred carbon source even for hydrocarbon degrading bacteria. Regardless of aeration regimes Triton X-100 was utilized within 48-72 h. Efficiency of diesel oil degradation was decreased in the presence of surfactant for aerobic conditions by approx. 25% reaching 60 instead of 80% noted for experiments without surfactant. No surfactant influence was observed for anaerobic conditions.
authors: Institute of Chemistry, Poznan University of Technology, Poznań, Poland.
authors: Bogdan
authors: B
authors: Wyrwas
authors: Łukasz
authors: Ł
authors: Chrzanowski
authors: Łukasz
authors: Ł
authors: Ławniczak
authors: Alicja
authors: A
authors: Szulc
authors: Paweł
authors: P
authors: Cyplik
authors: Wojciech
authors: W
authors: Białas
authors: Andrzej
authors: A
authors: Szymański
authors: Aleksandra
authors: A
authors: Hołderna-Odachowska
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005742:Gasoline; D006207:Half-Life; D017830:Octoxynol / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D005742:Gasoline; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol