title: Recent developments in the diagnosis of ectoparasite infections and disease through a better understanding of parasite biology and host responses.
abstract: Some conventional methods of diagnosis of ectoparasite infections can have low sensitivity and/or specificity. In addition, early infestations, sub-clinical and carrier hosts often go un-diagnosed, allowing infestations to spread. This review focuses on the important ectoparasites of human, livestock and companion animals for which improved diagnostic tools are either already in use, or in development. These advances in diagnostic technologies have resulted in improved treatment, control and preventative strategies for many ectoparasitic diseases. Immunodiagnostic methods have had a large impact, with the emergence of highly sensitive and specific enzyme-linked immunosorbent assays (ELISAs) for sarcoptic and psoroptic mange, with further improved tests in development. In the present review, the advantages and limitations of such tests are discussed and the potential for future development explored. The increasing use of molecular tools, for example, PCR and other molecular methods, has improved our understanding of the epidemiology of ectoparasitic diseases, with practical consequences for community-based control programmes. Recently, the identification of specific signalling pathways during the host response to ectoparasites has led to the identification of disease biomarkers which, along with new technologies, such as multiplexed assays and microfluidic platforms, could lead to more cost-effective, rapid and accurate diagnosis of infectious diseases.
authors: Moredun Research Institute, Pentlands Science Park, Midlothian EH26 0PZ, United Kingdom. Beth.Wells@moredun.ac.uk
authors: Beth
authors: B
authors: Wells
authors: Stewart T G
authors: ST
authors: Burgess
authors: Tom N
authors: TN
authors: McNeilly
authors: John F
authors: JF
authors: Huntley
authors: Alasdair J
authors: AJ
authors: Nisbet
mesh_terms: D000818:Animals; D004478:Ectoparasitic Infestations / Q000175:diagnosis* / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006790:Host-Parasite Interactions / Q000502:physiology*; D006801:Humans; D008925:Mites / Q000472:pathogenicity*