title: Analysis of the immune response to Neisseria meningitidis using a proteomics approach.
abstract: The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapter, we describe a protocol that combines two-dimensional (2D) SDS-PAGE of meningococcal outer membranes with western blotting of human antisera to identify proteins associated with the development of protective antibody responses. This methodology can identify putative vaccine candidates for incorporation in a multi-component serogroup B meningococcal vaccine.
authors: Division of Infection, Inflammation, and Immunity, Sir Henry Wellcome Laboratories, University of Southampton Medical School, Southampton, UK. J.N.Williams@soton.ac.uk
authors: Jeannette N
authors: JN
authors: Williams
authors: Myron
authors: M
authors: Christodoulides
authors: John E
authors: JE
authors: Heckels
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007525:Isoelectric Focusing / Q000379:methods; D009345:Neisseria meningitidis / Q000276:immunology*; D040901:Proteomics / Q000379:methods*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007106:Immune Sera