title: Canadian multicenter laboratory study for standardized second-line antimicrobial susceptibility testing of Mycobacterium tuberculosis.
abstract: The purpose of this study was to establish a standardized protocol for second-line antimicrobial susceptibility testing of Mycobacterium tuberculosis using the Bactec MGIT 960 system in Canadian laboratories. Four Canadian public health laboratories compared the susceptibility testing results of 9 second-line antimicrobials between the Bactec 460 and Bactec MGIT 960 systems. Based on the data generated, we have established that the Bactec MGIT 960 system provides results comparable to those obtained with the previous Bactec 460 method. The critical concentrations established for the testing of the antimicrobials used are as follows: amikacin, 1 μg/ml; capreomycin, 2.5 μg/ml; ethionamide, 5 μg/ml; kanamycin, 2.5 μg/ml; linezolid, 1 μg/ml; moxifloxacin, 0.25 μg/ml; ofloxacin, 2 μg/ml; p-aminosalicylic acid, 4 μg/ml; rifabutin, 0.5 μg/ml.
authors: National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St., Winnipeg, MB R3E 3P6, Canada. meenu.sharma@phac-aspc.gc.ca
authors: Meenu
authors: M
authors: Sharma
authors: Louise
authors: L
authors: Thibert
authors: Pamela
authors: P
authors: Chedore
authors: Cary
authors: C
authors: Shandro
authors: Frances
authors: F
authors: Jamieson
authors: Gregory
authors: G
authors: Tyrrell
authors: Sara
authors: S
authors: Christianson
authors: Hafid
authors: H
authors: Soualhine
authors: Joyce
authors: J
authors: Wolfe
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D002170:Canada; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards*; D009169:Mycobacterium tuberculosis / Q000187:drug effects*
chemical_list: D000995:Antitubercular Agents