title: Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440.
abstract: BACKGROUND
  Rhamnolipids are potent biosurfactants with high potential for industrial applications. However, rhamnolipids are currently produced with the opportunistic pathogen Pseudomonas aeruginosa during growth on hydrophobic substrates such as plant oils. The heterologous production of rhamnolipids entails two essential advantages: Disconnecting the rhamnolipid biosynthesis from the complex quorum sensing regulation and the opportunity of avoiding pathogenic production strains, in particular P. aeruginosa. In addition, separation of rhamnolipids from fatty acids is difficult and hence costly.
  
  
  RESULTS
  Here, the metabolic engineering of a rhamnolipid producing Pseudomonas putida KT2440, a strain certified as safety strain using glucose as carbon source to avoid cumbersome product purification, is reported. Notably, P. putida KT2440 features almost no changes in growth rate and lag-phase in the presence of high concentrations of rhamnolipids (> 90 g/L) in contrast to the industrially important bacteria Bacillus subtilis, Corynebacterium glutamicum, and Escherichia coli. P. putida KT2440 expressing the rhlAB-genes from P. aeruginosa PAO1 produces mono-rhamnolipids of P. aeruginosa PAO1 type (mainly C(10):C(10)). The metabolic network was optimized in silico for rhamnolipid synthesis from glucose. In addition, a first genetic optimization, the removal of polyhydroxyalkanoate formation as competing pathway, was implemented. The final strain had production rates in the range of P. aeruginosa PAO1 at yields of about 0.15 g/g(glucose) corresponding to 32% of the theoretical optimum. What's more, rhamnolipid production was independent from biomass formation, a trait that can be exploited for high rhamnolipid production without high biomass formation.
  
  
  CONCLUSIONS
  A functional alternative to the pathogenic rhamnolipid producer P. aeruginosa was constructed and characterized. P. putida KT24C1 pVLT31_rhlAB featured the highest yield and titer reported from heterologous rhamnolipid producers with glucose as carbon source. Notably, rhamnolipid production was uncoupled from biomass formation, which allows optimal distribution of resources towards rhamnolipid synthesis. The results are discussed in the context of rational strain engineering by using the concepts of synthetic biology like chassis cells and orthogonality, thereby avoiding the complex regulatory programs of rhamnolipid production existing in the natural producer P. aeruginosa.
authors: Institute for Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany.
authors: Andreas
authors: A
authors: Wittgens
authors: Till
authors: T
authors: Tiso
authors: Torsten T
authors: TT
authors: Arndt
authors: Pamela
authors: P
authors: Wenk
authors: Johannes
authors: J
authors: Hemmerich
authors: Carsten
authors: C
authors: Müller
authors: Rolf
authors: R
authors: Wichmann
authors: Benjamin
authors: B
authors: Küpper
authors: Michaela
authors: M
authors: Zwick
authors: Susanne
authors: S
authors: Wilhelm
authors: Rudolf
authors: R
authors: Hausmann
authors: Christoph
authors: C
authors: Syldatk
authors: Frank
authors: F
authors: Rosenau
authors: Lars M
authors: LM
authors: Blank
mesh_terms: D018533:Biomass; D005947:Glucose / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis*; D060847:Metabolic Engineering; D016958:Pseudomonas putida / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D006017:Glycolipids; C418382:rhamnolipid; D005947:Glucose