title: Development of two multiplex PCR systems for the analysis of 14 X-chromosomal STR loci in a southern Brazilian population sample.
abstract: We developed two multiplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-Multiplex 1 consisted of DXS6807, DXS6800, DXS7424, DXS101, GATA172D05 and HPRTB and X-Multiplex 2 consisted of DXS8378, DXS9898, DXS6801, DXS6809, DXS6789, DXS7133, DXS8377 and DXS7423. In addition, we present allele frequencies for these loci in a south Brazilian population comprising 124 females and 141 males and haplotype frequencies of linked markers for males. Hardy-Weinberg equilibrium (HWE) was tested in the female sample and no significant deviations were found after applying Bonferroni's correction. Linkage disequilibrium (LD) tests were performed for all pairs of loci and three significant results, out of 91 pairwise comparisons, were obtained. We did not find any evidence of linkage disequilibrium between close or linked markers. The power of discrimination in females (PD(F)) varied between 0.832 for DXS6801 and 0.987 for DXS8377. DXS6801 was the least informative marker (PIC = 0.605), while DXS8377 was the most polymorphic (PIC = 0.911), followed by DXS101 (PIC = 0.872). Genetic distances were estimated for each STR marker applying the calculation of F                         (ST) between our total sample and other studies from Brazil, Europe, Asia and Africa. The most distant populations were Japan, Korea, China, Ghana and Uganda.
authors: Postgraduate Program in Medicine, Medical Sciences, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. larisp@sinos.net
authors: Larissa Siqueira
authors: LS
authors: Penna
authors: Fernanda Gamio
authors: FG
authors: Silva
authors: Patricia Hartstein
authors: PH
authors: Salim
authors: Gisele
authors: G
authors: Ewald
authors: Mariana
authors: M
authors: Jobim
authors: José Antônio de Azevedo
authors: JA
authors: Magalhães
authors: Luiz Fernando
authors: LF
authors: Jobim
mesh_terms: D001483:Base Sequence; D001938:Brazil; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D041321:Chromosomes, Human, X / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics; D005784:Gene Amplification; D005787:Gene Frequency; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
chemical_list: D005819:Genetic Markers