title: Hepatoprotective effect of prolactin and cysteine contained in perfusion and preservation solutions on porcine liver stored in simple hypothermia.
abstract: BACKGROUND
  The aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers.
  
  
  METHODS
  We measured in the media of isolated livers stored for 24 hours in HTK (control group) or modified HTK+Cys (0.3 mmol/L)+PRL (3 IU/L study group) the amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid as well as Ca (II), Mg (II), Na (I) and K (I) ions during a 30-minute perfusion after 24 hours of storage.
  
  
  RESULTS
  All tested markers were released more slowly into HTK+Cys+PRL with less release of K(I) and Mg(II) and greater of Na(I) and Ca(II) ions.
  
  
  CONCLUSIONS
  Addition of the Cys and PRL to HTK positively affected 24-hour storage of isolated livers.
authors: Biochefa Pharmaceutical Research and Production Plant, Sosnowiec, Poland.
authors: F
authors: F
authors: Ryszka
authors: B
authors: B
authors: Dolińska
authors: A
authors: A
authors: Caban
authors: A
authors: A
authors: Ostróżka-Cieślik
authors: G
authors: G
authors: Budziński
authors: M
authors: M
authors: Krzysztofik
authors: G
authors: G
authors: Oczkowicz
authors: L
authors: L
authors: Cierpka
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D050377:Cold Ischemia; D003545:Cysteine / Q000008:administration & dosage*; D005260:Female; D005947:Glucose / Q000737:chemistry; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D016031:Liver Transplantation; D008353:Mannitol / Q000737:chemistry; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000737:chemistry; D010477:Perfusion; D011189:Potassium Chloride / Q000737:chemistry; D011343:Procaine / Q000737:chemistry; D011388:Prolactin / Q000008:administration & dosage*; D034421:Sus scrofa; D013997:Time Factors
chemical_list: C039984:Bretschneider cardioplegic solution; D002412:Cations; D019188:Organ Preservation Solutions; D019344:Lactic Acid; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D011388:Prolactin; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005947:Glucose; D003545:Cysteine