title: Intranasal inoculation with an adenovirus vaccine encoding ten repeats of Aβ3-10 induces Th2 immune response against amyloid-β in wild-type mouse.
abstract: To develop a safe and efficient vaccine for the treatment of Alzheimer's disease, we constructed a novel adenovirus vaccine encoding ten repeats of Aβ3-10 and CpG motif as an adjuvant and investigated the immune response in BALB/c mouse after intranasal inoculation with this vaccine. The Ad-10×Aβ3-10-CpG induces an IgG1 predominant humoral response and production of IL-4 and IL-10 in splenocytes in vitro, indicating a Th2-polarized immune response. Stimulation of splenocytes with Aβ3-10 peptide induces robust proliferation but not with full-length Aβ42 peptide, demonstrating that Ad-10×Aβ3-10-CpG does not induces Aβ42 specific T cell immune response. The findings raise the possibility that the adenovirus vaccine Ad-10×Aβ3-10-CpG could be a safe and effective alternative for immunotherapy in Alzheimer's disease.
authors: Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors: Yu
authors: Y
authors: Li
authors: Ying
authors: Y
authors: Ma
authors: Li-Xia
authors: LX
authors: Zong
authors: Xiao-Na
authors: XN
authors: Xing
authors: Sha
authors: S
authors: Sha
authors: Yun-Peng
authors: YP
authors: Cao
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology*; D000281:Administration, Intranasal; D000544:Alzheimer Disease / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D000818:Animals; D016764:Cell Polarity / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000821:virology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D016229:Amyloid beta-Peptides; C556062:IL10 protein, mouse; D014765:Viral Vaccines; D016753:Interleukin-10; D015847:Interleukin-4