title: Classification of Salmonella enterica serotypes from Australian poultry using repetitive sequence-based PCR.
abstract: OBJECTIVE
  To evaluate a semi-automated repetitive extragenic palindromic sequence-based PCR (rep-PCR) system for the classification of Salmonella serotypes from Australian poultry.
  
  
  RESULTS
  Using a DNA fingerprint library within the DiversiLab(®) System, four separate databases were constructed (serogroup B, C, E and Other). These databases contained 483 serologically confirmed (reference laboratory) Salmonella isolates. A blinded set of Salmonella cultures (n = 155) were typed by rep-PCR, matched against the internal library and compared with traditional serotyping. The predicted (Kullback-Leibler) serotype of 143 (92·3%) isolates matched traditional typing (P < 0·05). Of the 12 (7·7%) remaining isolates, ten (6·5%) resulted in 'No Match', one (0·65%) was incorrectly matched to the library (Salm. subsp 1 ser 4,12:-:-), and the other (0·65%) was referenced as Salm. ser. Sofia, whereas rep-PCR and in-house serotyping concurred as Salmonella serovar Typhimurium. Financial analysis showed higher material cost (215%) and a lower labour component (47·5%) for rep-PCR compared with serotyping.
  
  
  CONCLUSIONS
  The DiversiLab(®) System, with serogroup databases, was successfully implemented as an adjunct for reference serotyping of Salmonella enterica.
  
  
  CONCLUSIONS
  The DiversiLab(®) System platform is a cost-effective and easy-to-use system, which can putatively determine Salmonella enterica serotypes within a few hours.
authors: Birling Avian Laboratories, Bringelly, NSW, Australia.
authors: J W
authors: JW
authors: Chenu
authors: J M
authors: JM
authors: Cox
authors: A
authors: A
authors: Pavic
mesh_terms: D000818:Animals; D001315:Australia; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000592:standards*; D011200:Poultry; D015203:Reproducibility of Results; D012480:Salmonella Infections / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*; D012703:Serotyping