title: New microsatellite markers for Salvia officinalis (Lamiaceae) and cross-amplification in closely related species.
abstract: OBJECTIVE
  Microsatellite primers were identified to study the genetic diversity, population genetic structure, and relationships of common sage and closely related species.
  
  
  RESULTS
  Nine novel polymorphic trinucleotide microsatellite loci were identified for Salvia officinalis. A total of 127 alleles were observed. The observed and expected heterozygosity values ranged from 0.375 to 0.880 and from 0.624 to 0.931, respectively. Nine new and 11 previously published microsatellite primers were tested for cross-amplification. The species with the most successful cross-amplification was S. fruticosa.
  
  
  CONCLUSIONS
  The nine new microsatellite markers will be useful in genetic studies of wild and cultivated populations of common sage and are potentially useful in genetic studies of closely related species.
authors: University of Zagreb, Faculty of Science, Division of Biology, Department of Botany, Marulicev trg 9a, HR 10000 Zagreb, Croatia.
authors: Ivan
authors: I
authors: Radosavljević
authors: Jernej
authors: J
authors: Jakse
authors: Branka
authors: B
authors: Javornik
authors: Zlatko
authors: Z
authors: Satovic
authors: Zlatko
authors: Z
authors: Liber
mesh_terms: D000483:Alleles; D017931:DNA Primers; D018744:DNA, Plant; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D027542:Rosmarinus / Q000235:genetics*; D027543:Salvia officinalis / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant