title: Pullulan hydrogels improve mesenchymal stem cell delivery into high-oxidative-stress wounds.
abstract: Cell-based therapies for wound repair are limited by inefficient delivery systems that fail to protect cells from the acute inflammatory environment. Here, a biomimetic hydrogel system is described that is based on the polymer pullulan, a carbohydrate glucan known to exhibit potent antioxidant capabilities. It is shown that pullulan hydrogels are an effective cell delivery system and improve mesenchymal stem cell survival and engraftment in high-oxidative-stress environments. The results suggest that glucan hydrogel systems may prove beneficial for progenitor-cell-based approaches to skin regeneration.
authors: Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA.
authors: Victor W
authors: VW
authors: Wong
authors: Kristine C
authors: KC
authors: Rustad
authors: Jason P
authors: JP
authors: Glotzbach
authors: Michael
authors: M
authors: Sorkin
authors: Mohammed
authors: M
authors: Inayathullah
authors: Melanie R
authors: MR
authors: Major
authors: Michael T
authors: MT
authors: Longaker
authors: Jayakumar
authors: J
authors: Rajadas
authors: Geoffrey C
authors: GC
authors: Gurtner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D032701:Biomimetics / Q000379:methods*; D005260:Female; D005936:Glucans / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D020100:Hydrogels / Q000138:chemical synthesis / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D059630:Mesenchymal Stem Cells; D051379:Mice; D008815:Mice, Inbred Strains; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D017695:Soft Tissue Injuries / Q000378:metabolism / Q000628:therapy; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D005936:Glucans; D020100:Hydrogels; D017382:Reactive Oxygen Species; C009109:pullulan; D006861:Hydrogen Peroxide