title: Bcl-2 modulates endoplasmic reticulum and mitochondrial calcium stores in PC12 cells.
abstract: Endoplasmic reticulum (ER) and mitochondria are intracellular organelles and their interactions are directly involved in different processes such as Ca(2+) signaling in cell survival and death mechanisms. Bcl-2 is an anti-apoptotic protein intrinsically related to ER and mitochondria, modulating Ca(2+) content in these organelles. We investigated the effects of Bcl-2 overexpression on ER and mitochondrial Ca(2+) dynamics in PC12 cells. Bcl-2 overexpressing and control cells were loaded with Fura 2/AM and stimulated with different drugs. Results showed that in Bcl-2 cells, ACh induced a lower Ca(2+) response compared to control. Ca(2+) release induced by TG was decreased in Bcl-2 cells, however, it was greater in Caff induced Ca(2+) rise. In addition, FCCP induced a higher Ca(2+) release in Bcl-2 cells. These results suggest that Bcl-2 overexpression modulate the ER Ca(2+) pools differently and the release of ER Ca(2+) may increase mitochondrial Ca(2+) accumulation. These alterations of intracellular Ca(2+) stores are important mechanisms for the control of Ca(2+) signaling.
authors: Departamento de Farmacologia, Universidade Federal de São Paulo (UNIFESP/EPM), Rua Três de Maio 100, São Paulo-SP, CEO: 04044-020, Brazil.
authors: Hanako
authors: H
authors: Hirata
authors: Guiomar S
authors: GS
authors: Lopes
authors: Aron
authors: A
authors: Jurkiewicz
authors: Lucia
authors: L
authors: Garcez-do-Carmo
authors: Soraya S
authors: SS
authors: Smaili
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology*; D051381:Rats; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D002110:Caffeine; D019284:Thapsigargin; D000109:Acetylcholine; D002118:Calcium