title: Anoctamins and gastrointestinal smooth muscle excitability.
abstract: Interstitial cells of Cajal (ICC) generate electrical pacemaker activity in gastrointestinal smooth muscles. We investigated whether Tmem16a, which encodes anoctamin 1 (ANO1), a Ca(2+)-activated Cl(-) channel, might be involved in pacemaker activity in ICC. The Tmem16a transcripts and ANO1 were expressed robustly in GI muscles, specifically in ICC in murine, non-human primate (Macaca fascicularis) and human GI tracts. Splice variants of Tmem16a, as well as other paralogues of the Tmem16 family, were expressed in gastrointestinal muscles. Calcium-activated Cl(-) channel blocking drugs, niflumic acid and DIDS blocked slow waves in intact muscles of mouse, primate and human small intestine and stomach. Slow waves failed to develop in Tmem16a knock-out mice (Tmem16a(tm1Bdh/tm1Bdh)). The pacemaker mechanism was investigated in isolated ICC from transgenic mice with constitutive expression of copepod super green fluorescent protein (copGFP). Depolarization of ICC activated inward currents due to a Cl(-)-selective conductance. Removal of extracellular Ca(2+), replacement of Ca(2+) with Ba(2+), or extracellular Ni(2+) (30 μM) blocked the inward current. Single Ca(2+)-activated Cl(-) channels with a unitary conductance of 7.8 pS were resolved in excised patches from ICC. The inward current was blocked in a concentration-dependent manner by niflumic acid (IC(50) = 4.8 μM). The role of ANO1 in cholinergic responses in ICC was also investigated. Carbachol activated Ca(2+)-activated Cl(-) currents in ICC, and responses to cholinergic nerve stimulation were blocked by niflumic acid in intact muscles. Anoctamin 1 is a prominent conductance in ICC, and these channels appear to be involved in pacemaker activity and in responses to enteric excitatory neurotransmitters.
authors: Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno, NV 89557, USA. ksanders@medicine.nevada.edu
authors: Kenton M
authors: KM
authors: Sanders
authors: Mei Hong
authors: MH
authors: Zhu
authors: Fiona
authors: F
authors: Britton
authors: Sang Don
authors: SD
authors: Koh
authors: Sean M
authors: SM
authors: Ward
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D056885:Interstitial Cells of Cajal / Q000378:metabolism / Q000502:physiology; D009130:Muscle, Smooth / Q000378:metabolism / Q000502:physiology*
chemical_list: D018118:Chloride Channels