title: HAT4, a Golgi apparatus-anchored B-type histone acetyltransferase, acetylates free histone H4 and facilitates chromatin assembly.
abstract: Histone acetyltransferases (HATs) are an essential regulatory component in chromatin biology. Unlike A-type HATs, which are found in the nucleus and utilize nucleosomal histones as substrates and thus primarily function in transcriptional regulation, B-type HATs have been characterized as cytoplasmic enzymes that catalyze the acetylation of free histones. Here, we report on a member of the GCN5-related N-acetyltransferase superfamily and another B-type HAT, HAT4. Interestingly, HAT4 is localized in the Golgi apparatus and displays a substrate preference for lysine residues of free histone H4, including H4K79 and H4K91, that reside in the globular domain of H4. Significantly, HAT4 depletion impaired nucleosome assembly, inhibited cell proliferation, sensitized cells to DNA damage, and induced cell apoptosis. Our data indicate that HAT4 is an important player in the organization and function of the genome and may contribute to the diversity and complexity of higher eukaryotic organisms.
authors: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China.
authors: Xiaohan
authors: X
authors: Yang
authors: Wenhua
authors: W
authors: Yu
authors: Lei
authors: L
authors: Shi
authors: Luyang
authors: L
authors: Sun
authors: Jing
authors: J
authors: Liang
authors: Xia
authors: X
authors: Yi
authors: Qian
authors: Q
authors: Li
authors: Yu
authors: Y
authors: Zhang
authors: Fen
authors: F
authors: Yang
authors: Xiao
authors: X
authors: Han
authors: Di
authors: D
authors: Zhang
authors: Jie
authors: J
authors: Yang
authors: Zhi
authors: Z
authors: Yao
authors: Yongfeng
authors: Y
authors: Shang
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002843:Chromatin / Q000737:chemistry*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism*; D004249:DNA Damage; D015971:Gene Expression Regulation, Enzymologic; D006056:Golgi Apparatus / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000737:chemistry*; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D051548:Histone Acetyltransferases