title: Alginate films containing Lactobacillus plantarum as wound dressing for prevention of burn infection.
abstract: The topical application of lactobacilli is receiving attention for the prevention of superficial skin and burn wound infections. We studied the properties of Lactobacillus plantarum immobilized in calcium alginate films and investigated the antibacterial activity of these films in a model burn wound in rats. A multiresistant clinical isolate, VIM-2-metallo-β-lactamase-producing Pseudomonas aeruginosa, was used as the indicator strain. Films incorporating L. plantarum at cell concentrations of 10(8)cfu/mL caused a reduction of 5-6 log(10) in P. aeruginosa in the model burn wounds. L. plantarum immobilized in freeze-dried calcium alginate films remained viable during six months of storage at 4 °C.
authors: iMed - Research Institute for Medicines and Pharmaceutical Sciences, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal.
authors: M I
authors: MI
authors: Brachkova
authors: P
authors: P
authors: Marques
authors: J
authors: J
authors: Rocha
authors: B
authors: B
authors: Sepodes
authors: M A
authors: MA
authors: Duarte
authors: J F
authors: JF
authors: Pinto
mesh_terms: D000287:Administration, Topical; D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D000898:Antibiosis; D058491:Bacterial Load; D001458:Bandages; D002056:Burns / Q000628:therapy*; D018914:Cells, Immobilized / Q000502:physiology; D004195:Disease Models, Animal; D004355:Drug Stability; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008297:Male; D050296:Microbial Viability; D011552:Pseudomonas Infections / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity; D051381:Rats; D017208:Rats, Wistar; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid