title: Gene expression profiling of in Moniezia expansa at different developmental proglottids using cDNA microarray.
abstract: Gene expression profiles of Moniezia expansa proglottids at varying developmental stages were analysed using cDNA microarray. A total of 4,056 spots, including full length and partial complementary DNAs that represent novel, known, and control genes, were studied. Results indicated an up-regulation of 55 genes in immature proglottids, 134 genes in mature proglottids and 103 genes in gravid proglottids were up-regulated, and a down-regulation of 7 genes in immature proglottids, 68 genes in mature proglottids and 78 genes in gravid proglottids compared to controls (scolex-neck proglottids). Many of these genes were identified as transcription factors and were involved in functions such as metabolism, transport, protein biosynthesis, apoptosis, cell differentiation, cell communication and nucleic acid binding. Expression level alterations in UBE2A, Cavβ, RAD51, DAZ, PKAc and 2 unknown genes were confirmed by real-time quantitative polymerase chain reaction (RT-PCR). The complete microarray data set has been deposited in the NCBI Gene Expression Omnibus, GEO Series accession number GSE13982. Results provide a gene expression profile at various development stages of M. expansa proglottids, which prove invaluable in understanding the pathogenesis of the tapeworm and studying the genes concerned with reproductive organ development.
authors: Genetic Engineering Laboratory, The Breed & Biotechnology Key Laboratory of Sheep in XinJiang Bingtuan/Animal Husbandry and Veterinary Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, People's Republic of China. boxinwen@yahoo.com.cn
authors: Xinwen
authors: X
authors: Bo
authors: Wenjuan
authors: W
authors: Zhao
authors: Hui
authors: H
authors: Zhang
authors: Lichao
authors: L
authors: Kang
authors: Xinhua
authors: X
authors: Wang
mesh_terms: D000825:Animal Structures / Q000254:growth & development* / Q000378:metabolism*; D000818:Animals; D002589:Cestoda / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction