title: Development of a human-murine chimeric immunoglobulin M for use in the serological detection of human alphavirus antibodies.
abstract: Diagnosis of human alphaviral infections relies on serological techniques, such as the immunoglobulin M antibody capture-enzyme-linked immunosorbent assay (MAC-ELISA). We have humanized the alphavirus broadly cross-reactive murine monoclonal antibody 1A4B-6 to create a reagent capable of replacing human positive sera in the MAC-ELISA for diagnosis of human alphaviral infections.
authors: Arboviral Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, 3150 Rampart Road, Fort Collins, CO 80521, USA. epx1@cdc.gov
authors: Brett A
authors: BA
authors: Thibodeaux
authors: Nathan M
authors: NM
authors: Liss
authors: Amanda N
authors: AN
authors: Panella
authors: John T
authors: JT
authors: Roehrig
mesh_terms: D000524:Alphavirus / Q000276:immunology*; D018354:Alphavirus Infections / Q000175:diagnosis*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000235:genetics / Q000276:immunology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011994:Recombinant Proteins