title: Selective fluorometric detection of pyrophosphate and stringent alarmone with copper(II)-2,6-bis(2-benzimidazolyl)pyridine complex.
abstract: Pyrophosphate (PPi) is involved in lots of anabolism and bioenergetic processes in organisms and possesses important biological functions so that its detection is very significant. Here, we developed a selective fluorometric detection method for PPi with a copper(II) complex of 2,6-bis(2-benzimidazolyl)pyridine (bbimp), and then applied it to the detection of bacterial alarmone ppGpp. bbimp has the fluorescence emission at 395 nm, but the bbimp-Cu(2+) complex is hardly fluorescent because the intrinsic fluorescence of bbimp is effectively quenched by Cu(2+). With the addition of PPi, however, the fluorescence emission of bbimp turns on with a 2 nm red-shift, and has a linear relationship with PPi in the range of 3-90 μmol/L. This method has good selectivity for PPi over other anions especially those phosphate-containing anions such as ATP, UTP, CTP, GTP, GDP, and PO(4)(3-).
authors: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
authors: Xi Juan
authors: XJ
authors: Zhao
authors: Cheng Zhi
authors: CZ
authors: Huang
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003300:Copper / Q000032:analysis*; D011756:Diphosphates / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: C500137:2,6-bis(benzimidazol-2-yl)pyridine; D001562:Benzimidazoles; D011756:Diphosphates; D011725:Pyridines; D003300:Copper