title: Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
abstract: BACKGROUND
  Polymorphic differences between donor and recipient human leukocyte antigen class I molecules can result in graft-versus-host disease due to distinct peptide presentation. As part of the peptide-loading complex, tapasin plays an important role in selecting peptides from the pool of potential ligands. Class I polymorphisms can significantly alter the tapasin-mediated interaction with the peptide-loading complex and although most class I allotypes are highly dependent upon tapasin, some are able to load peptides independently of tapasin. Several human leukocyte antigen B*44 allotypes differ exclusively at position 156 (B*44:02(156Asp), 44:03(156Leu), 44:28(156Arg), 44:35(156Glu)). From these alleles, only the high tapasin-dependency of human leukocyte antigen B*44:02 has been reported.
  
  
  METHODS
  We investigated the influence of position 156 polymorphisms on both the requirement of tapasin for efficient surface expression of each allotype and their peptide features. Genes encoding human leukocyte antigen B*44 variants bearing all possible substitutions at position 156 were lentivirally transduced into human leukocyte antigen class I-negative LCL 721.221 cells and the tapasin-deficient cell line LCL 721.220.
  
  
  RESULTS
  Exclusively human leukocyte antigen B*44:28(156Arg) was expressed on the surface of tapasin-deficient cells, suggesting that the remaining B*44/156 variants are highly tapasin-dependent. Our computational analysis suggests that the tapasin-independence of human leukocyte antigen B*44:28(156Arg) is a result of stabilization of the peptide binding region and generation of a more peptide receptive state. Sequencing of peptides eluted from human leukocyte antigen B*44 molecules by liquid chromatography-electrospray ionization-mass spectrometry (LTQ-Orbitrap) demonstrated that both B*44:02 and B*44:28 share the same overall peptide motif and a certain percentage of their individual peptide repertoires in the presence and/or absence of tapasin.
  
  
  CONCLUSIONS
  Here we report for the first time the influence of position 156 on the human leukocyte antigen/tapasin association. Additionally, the results of peptide sequencing suggest that tapasin chaperoning is needed to acquire peptides of unusual length.
authors: Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
authors: Soumya
authors: S
authors: Badrinath
authors: Philippa
authors: P
authors: Saunders
authors: Trevor
authors: T
authors: Huyton
authors: Susanne
authors: S
authors: Aufderbeck
authors: Oliver
authors: O
authors: Hiller
authors: Rainer
authors: R
authors: Blasczyk
authors: Christina
authors: C
authors: Bade-Doeding
mesh_terms: D000483:Alleles; D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D019295:Computational Biology / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D020868:Gene Silencing; D057809:HEK293 Cells; D059948:HLA-B44 Antigen / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011110:Polymorphism, Genetic; D011485:Protein Binding
chemical_list: D000939:Epitopes; D059948:HLA-B44 Antigen; D026901:Membrane Transport Proteins; D010455:Peptides; C107762:tapasin