title: Self-assemble gene delivery system for molecular targeting using nucleic acid aptamer.
abstract: We have developed a novel vector constructed with pDNA, polyethylenimine (PEI), and mucin 1 (MUC1) aptamer for tumor-targeted gene delivery. The MUC1 aptamer and non-specific aptamer were employed to coat the pDNA/PEI complexes electrostatically and stable nanoparticles were formed. The addition of a non-specific aptamer to the pDNA/PEI complex decreased gene expression in the human lung cancer cell line, A549 cells expressing MUC1 regularly. At the same time, the pDNA/PEI/MUC1 aptamer complex showed higher gene expression than pDNA/PEI/non-specific aptamer complex. Furthermore, the pDNA/PEI/MUC1 aptamer complex showed markedly high gene expression in tumor-bearing mice; thus, pDNA/PEI/MUC1 aptamer complexes are useful as a tumor-targeted gene delivery system with high transfection efficiency.
authors: Department of Hospital Pharmacy, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
authors: Tomoaki
authors: T
authors: Kurosaki
authors: Norihide
authors: N
authors: Higuchi
authors: Shigeru
authors: S
authors: Kawakami
authors: Yuriko
authors: Y
authors: Higuchi
authors: Tadahiro
authors: T
authors: Nakamura
authors: Takashi
authors: T
authors: Kitahara
authors: Mitsuru
authors: M
authors: Hashida
authors: Hitoshi
authors: H
authors: Sasaki
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection
chemical_list: D052157:Aptamers, Nucleotide; D011094:Polyethyleneimine