title: Balancing image quality and compression factor for special stains whole slide images.
abstract: The objective is to find a practical balance between quality and performance for daily high volume whole slide imaging. We evaluated whole slide images created by various scanners at different compression factors to determine the best suitable quality factor (QF) needed for pathological images of special stains.
  
  
  METHODS
  We scanned two sets of eight special stains slides each at 0.50 μm/pixel resolution in Hamamatsu scanner at six and five QF levels respectively to generate 72 images which were observed at a calibrated monitor by imaging specialists, a histo-technician, and a pathologist to find the most suitable QF level for special stains in digital slides.
  
  
  RESULTS
  Most special stains images were acceptable at QF 30 except for the stain Reticulin where the lowest acceptable QF was 50. The compression of images from QF 90 to QF 50 reduced the size of the images by 62.73%.
  
  
  CONCLUSIONS
  0.50 μm/pixel images at QF 50 or above were found suitable 12 special stain.
authors: NEC Laboratories America, Inc., Princeton, NJ, USA. asharma@neclabs.com
authors: Anurag
authors: A
authors: Sharma
authors: Pinky
authors: P
authors: Bautista
authors: Yukako
authors: Y
authors: Yagi
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods* / Q000592:standards*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods* / Q000592:standards*; D013194:Staining and Labeling / Q000379:methods*