title: Reactive oxygen species downregulate catalase expression via methylation of a CpG island in the Oct-1 promoter.
abstract: Reactive oxygen species (ROS) caused oxidative stress plays a key role in carcinogenesis. The POU domain transcription factor Oct-1 and catalase is closely associated with ROS. However, a correlation between these two key proteins has not been demonstrated before. In this report, we show that Oct-1 acts as an activator of catalase, by binding to the catalase promoter in hepatocellular carcinoma (HCC) cell lines. In addition, we suggest that Oct-1 is downregulated by ROS via CpG island methylation in its promoter. These findings contribute to a better understanding of the epigenetic changes induced by ROS in the process of carcinogenesis.
authors: The Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
authors: Xiaoyuan
authors: X
authors: Quan
authors: Seung-Oe
authors: SO
authors: Lim
authors: Guhung
authors: G
authors: Jung
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000187:drug effects / Q000235:genetics*; D019175:DNA Methylation / Q000187:drug effects*; D000077209:Decitabine; D015536:Down-Regulation / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D009361:Neoplasm Invasiveness; D050811:Octamer Transcription Factor-1 / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000494:pharmacology*
chemical_list: D050811:Octamer Transcription Factor-1; D017382:Reactive Oxygen Species; D000077209:Decitabine; D002374:Catalase; D001374:Azacitidine; D000111:Acetylcysteine