title: Obesity associated pathophysiological & histological changes in WNIN obese mutant rats.
abstract: OBJECTIVE
  WNIN/Ob (obese and euglycaemic) and WNIN/GR-Ob (obesity with impaired glucose tolerance), were isolated and established from Wistar rat stock (WNIN). Both strains showed physical, physiological and biochemical indices related to obesity. We present here haematology, histology and pathophysiological changes between the phenotypes of these strains, lean (+/+), carrier (+/-) and obese (-/-).
  
  
  METHODS
  A total of 72 animals of equal gender consisting of three phenotypes were used for the study. Haematology, organ weights were measured and histopathology of the tissues studied using standard procedures. In 12 lean and obese rats (equal gender) of WNIN/GR-Ob group morphometry of pancreatic islets was done immunohistochemically (IHC).
  
  
  RESULTS
  Obese rats of both the strains showed normal haematology (except low platelet count), but exhibited changes in the organ weights and in histopathology in organs like liver, kidney, brain and testis/ovary. Hyperplasia of adipocytes was seen in obese rats as compared to lean and carrier. IHC of obese rat pancreas showed that both islet density and volume were significantly (P<0.05) increased compared to lean littermates.
  
  
  CONCLUSIONS
  The histological and pathophysiological changes seen in these mutants were in tune with obese phenotype exhibited by these animals.
authors: National Centre for Laboratory Animal Sciences (ICMR), Hyderabad, India. hsnemani2000@yahoo.com
authors: Nemani
authors: N
authors: Harishankar
authors: Putcha Uday
authors: PU
authors: Kumar
authors: Boindala
authors: B
authors: Sesikeran
authors: Nappanveettil
authors: N
authors: Giridharan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose; D001823:Body Composition; D001947:Breeding / Q000379:methods; D004553:Electric Conductivity; D005260:Female; D018149:Glucose Intolerance / Q000503:physiopathology*; D007328:Insulin / Q000097:blood; D008297:Male; D009765:Obesity / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D010641:Phenotype; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007328:Insulin