title: An association between TRP64ARG polymorphism of the B3 adrenoreceptor gene and some metabolic disturbances.
abstract: BACKGROUND
  B3 adrenoreceptors (ADRB3) are abundant in adipose tissue and play the role in its metabolism and lipolysis. Some variants of the ADRB3 gene may predispose subjects for the development obesity and metabolic abnormalities in the setting of modern sedentary lifestyle. ADRB3 gene polymorphism association with metabolic disturbances has never been studied before in the ethnic Kyrgyz population.
  
  
  OBJECTIVE
  To study an association between Trp64Arg polymorphism of the ADRB3 and metabolic syndrome (MS) components in an ethnic Kyrgyz group.
  
  
  METHODS
  213 Ethnic Kyrgyz volunteers over the age of 30 were enrolled in the study. The assessment plan for each individual comprised of general physical and anthropometric exams as well as laboratory tests (glucose, lipid panel, insulin) and genotyping by Trp64Arg polymorphism of the ADRB3. MS diagnosis was consistent with modified ATP III criteria (2005). Logistic regression analysis was performed to test the potential independent association between Arg64 allele with obesity, abdominal obesity (AO) and arterial hypertension (AH).
  
  
  RESULTS
  Trp64Arg polymorphism of the ADRB3 was assessed in 213 individuals (145 men, 68 women) aged 30-73 (mean age 50.7 ± 7.6). Arg64 allele frequency was 0.239; ADRB3 genotype distribution among participants was: Trp64 homozygotes 54.5%, Trp64Arg 43.2% and Arg64 homozygotes 2.3%. There was an association between Trp64Arg и Arg64Arg genotypes and higher BMI, WC and obesity frequency (p < 0.00009), AO (p < 0.01), type 2 diabetes mellitus (DM) (p < 0.005) and lower high density cholesterol (HDL-C) level (p < 0.03). The logistic regression analysis showed the correlation of the Arg64 allele with obesity (OR 3.159; 95% CI 1.789-5.577) and AO (OR 1.973; 95% CI 1.118-3.481). The association between Arg64 allele and AH lost its significance after adjustment for obesity.
  
  
  CONCLUSIONS
  Arg64 allele of the ADRB3 gene in the studied group has an association with MS components such as obesity, AO and decreased HDL-C level.
authors: National Centre of Cardiology and Internal medicine named by M. Mirrakhimov, T.Moldo 3, Bishkek, 720040, Kyrgyzstan. amirrakhimov1@gmail.com
authors: Aibek E
authors: AE
authors: Mirrakhimov
authors: Alina S
authors: AS
authors: Kerimkulova
authors: Olga S
authors: OS
authors: Lunegova
authors: Cholpon B
authors: CB
authors: Moldokeeva
authors: Yulia V
authors: YV
authors: Zalesskaya
authors: Samai S
authors: SS
authors: Abilova
authors: Nurmira A
authors: NA
authors: Sovhozova
authors: Almaz A
authors: AA
authors: Aldashev
authors: Erkin M
authors: EM
authors: Mirrakhimov
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001120:Arginine / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics; D005260:Female; D056726:Genetic Association Studies / Q000379:methods; D006801:Humans; D007707:Kyrgyzstan / Q000208:ethnology; D008297:Male; D024821:Metabolic Syndrome / Q000208:ethnology / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000208:ethnology / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D014364:Tryptophan / Q000235:genetics*
chemical_list: D022702:Receptors, Adrenergic, beta-3; D014364:Tryptophan; D001120:Arginine