title: Biopolymers production from mixed cultures and pyrolysis by-products.
abstract: Polyhydroxyalkanoates (PHAs) production from low value substrates and/or byproducts represents an economical and environmental promising alternative to established industrial manufacture methods. Bio-oil resulting from the fast-pyrolysis of chicken beds was used as substrate to select a mixed microbial culture (MMC) able to produce PHA under feast/famine conditions. In this study a maximum PHA content of 9.2% (g/g cell dry weight) was achieved in a sequencing batch reactor (SBR) operated for culture selection. The PHA obtained with bio-oil as a carbon source was a copolymer composed by 70% of hydroxybutyrate (HB) and 30% of hydroxyvalerate (HV) monomers. Similar results have been reported by other studies that use real complex substrates for culture selection indicating that bio-oil can be a promising feedstock to produce PHAs using MMC. To the best of our knowledge this is the first study that demonstrated the use of bio-oil resulting from fast pyrolysis as a possibly feedstock to produce short chain length polyhydroxyalkanoates.
authors: REQUIMTE/CQFB, Chemistry Department, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors: R
authors: R
authors: Moita
authors: P C
authors: PC
authors: Lemos
mesh_terms: D000064:Acclimatization; D001419:Bacteria / Q000166:cytology / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001704:Biopolymers / Q000096:biosynthesis*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D006003:Glycogen / Q000032:analysis; D054813:Polyhydroxyalkanoates / Q000032:analysis; D013696:Temperature; D014835:Volatilization
chemical_list: D001704:Biopolymers; D054813:Polyhydroxyalkanoates; D006003:Glycogen