title: Expression of miR-146a and miR-155 in the urinary sediment of systemic lupus erythematosus.
abstract: We studied the levels of miR-146a and miR-155 in the urine sediment of SLE patients. The levels of miR-146a and miR-155 in the urine sediment of 40 SLE patients who were receiving calcitriol treatment and 13 healthy controls were determined with real-time quantitative polymerase chain reaction. The levels of urinary miR-146a and miR-155 in patients with SLE were significantly higher than that in healthy controls. Calcitriol treatment reduced the levels of urinary miR-155 in patients with SLE. The level of urinary miR-146a significantly correlated with estimated glomerular filtration rate (r = 0.242, P = 0.008). The level of urinary miR-155 significantly correlated with proteinuria (r = 0.407, P < 0.001) and systemic lupus erythematosus disease activity index (r = 0.278, P = 0.002). The level of urinary miR-146a reversely correlated with the urinary expression of TNF-α (r = -0.247, P = 0.012). Our results suggested that miR-146a and miR-155 might play important roles in the pathophysiology of SLE and the levels of urinary miR-146a and miR-155 could be used as potential markers for diagnosis, disease activity, and therapeutic response.
authors: Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors: Gang
authors: G
authors: Wang
authors: Lai-Shan
authors: LS
authors: Tam
authors: Bonnie Ching-Ha
authors: BC
authors: Kwan
authors: Edmund Kwok-Ming
authors: EK
authors: Li
authors: Kai-Ming
authors: KM
authors: Chow
authors: Cathy Choi-Wan
authors: CC
authors: Luk
authors: Philip Kam-Tao
authors: PK
authors: Li
authors: Cheuk-Chun
authors: CC
authors: Szeto
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D002117:Calcitriol / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy / Q000235:genetics / Q000652:urine*; D008297:Male; D035683:MicroRNAs / Q000652:urine*; D008875:Middle Aged; D011507:Proteinuria / Q000235:genetics / Q000652:urine*; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; C542542:MIRN146 microRNA, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D002117:Calcitriol