title: Strains of Klebsiella pneumoniae producing extended spectrum beta-lactamases, isolated from organ recipients.
abstract: OBJECTIVE
  The aims of this work were to define the effectiveness of identification of the extended-spectrum beta-lactamases (ESBL) phenotype, and to define the genotype of Klebsiella pneumoniae β-lactamase.
  
  
  METHODS
  We identified ESBL phenotypes in 110 strains of K pneumoniae isolated from samples from patients of transplantation wards, using the double-disk synergy test (DDST). For the chosen strains, polymerase chain reaction (PCR) was applied to detect genes determining SHV, CTX-M, and TEM.
  
  
  RESULTS
  We showed synergism of clavulanic acid and investigated antibiotics including ceftazidime (89.1%), cefotaxime (80%), and aztreonam (82.7%) against ESBL-positive strains PCR revealed that TEM and CTX-M were present in 88.89% of strains.
  
  
  CONCLUSIONS
  The ESBL mechanism of resistance is frequent among K pneumoniae strains isolated from transplant recipients. Strains with simultaneous synthesis of more than one beta-lactamase predominated.
authors: Department of Medical Microbiology, the Medical University of Warsaw, Poland.
authors: S Walter
authors: SW
authors: de Walthoffen
authors: A
authors: A
authors: Mlynarczyk
authors: A
authors: A
authors: Sawicka-Grzelak
authors: M
authors: M
authors: Durlik
authors: L
authors: L
authors: Paczek
authors: A
authors: A
authors: Chmura
authors: M
authors: M
authors: Ciszek
authors: L
authors: L
authors: Chabros
authors: T
authors: T
authors: Baczkowska
authors: G
authors: G
authors: Mlynarczyk
mesh_terms: D003428:Cross Infection / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D019737:Transplants / Q000009:adverse effects / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases