title: Comparison of RAPD and ISSR  markers for assessment of genetic diversity among endangered rare Dalbergia  oliveri (Fabaceae) genotypes in Vietnam.
abstract: Dalbergia  oliveri is a leguminous tree of the Fabaceae family. This  species is popular and valuable in Vietnam and is currently listed on the  Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques  using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a  comparative analysis of genetic diversity in this species. Fifty-six  polymorphic primers (29 RAPD and 27 ISSR) were used. The RAPD primers produced 63  bands across 35 genotypes, of which 24 were polymorphic. The number of  amplified bands varied from one to four, with a size range from 250 to 1400 bp.  The percentage polymorphism ranged from 0 to 75. Amplification of genomic DNA  of the 35 genotypes, using ISSR analysis, yielded 104 fragments, of which 63  were polymorphic. The number of amplified fragments using ISSR primers ranged  from one to nine and varied in size from 250 to 1500 bp. The percentage  polymorphism ranged from 0 to 100. ISSR markers were relatively more efficient  than RAPDs. The mental test between two Jaccard's similarity matrices gave r ≥0.802,  showing good fit correlation between ISSRs and RAPDs. Clustering of isolates  remained more or less the same for RAPDs compared to combined RAPD and ISSR  data. The similarity coefficient ranged from 0.785 to 1.000, 0.698 to 0.956 and  0.752 to 0.964 with RAPD, ISSR, and the combined RAPD-ISSR dendrogram,  respectively.
authors: Department of Experimental Taxonomy and Genetic Diversity, Vietnam National Museum of Nature, Vietnam Academy for Science and Technology, Hanoi, Vietnam. dinhthiphong@hotmail.com
authors: D T
authors: DT
authors: Phong
authors: V T T
authors: VT
authors: Hien
authors: T T V
authors: TT
authors: Thanh
authors: D V
authors: DV
authors: Tang
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D035782:Dalbergia / Q000235:genetics*; D056727:Endangered Species; D005819:Genetic Markers; D005838:Genotype; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014744:Vietnam
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers