title: Effects of tongmai huoxue yin (see text) on tumor necrosis factor-alpha in the acute cerebral ischemia model rat.
abstract: OBJECTIVE
  To observe the interfering action of Tongmai Huoxue Yin (see text) on the acute cerebral ischemia model rat.
  
  
  METHODS
  Total 60 SD rats, 30 females and 30 males, were randomly divided into 4 groups, sham-operation group, model group, Nimodipine group and Tongmai Huoxue Yin group, 15 rats in each group. The acute cerebral ischemia rat model was duplicated, the middle cerebral artery (MCA) were ligated and the thread was inserted for the rats in the model group, Nimodipine group and Tongmai Huoxue Yin group, for the rats in the sham-operation group, the arteries were separated without ligature and the thread was not inserted. After the modeling has succeed, the water-decocted concentrated solution of 20-fold Tongmai Huoxue Yin clinical dosage was intragastrically administrated in a dose of 3 mL/100 g d divided into twice, 1.5 mL/100 g once. Distilled water 3 mL/100 g x d was intragastrically administrated, 1.5 mL/100 g once, for the rat in the model group, Nimodipne suspension 3 mL/100 g x d (0.6 mg / 100 g) for the Nimodipine group and 3 mL/100 g x d (5.4 g/100 g) for the Tongmai Huoxue Yin group, no drugs for the sham-operation group. And changes of tumor necrosis factor-alpha (TNF-alpha) contents in the serum and brain tissue were investigated.
  
  
  RESULTS
  Compared with the model group, compared with the sham-operation group, serum TNF-alpha content at 5 h of focal cerebral ischemic ischemia in the model group started to increase and reached to the high peak at 12 h, but in both the Tongmai Huoxue Yin group and the Nimodipine group decreased in varying degrees at the same time; compared with the sham-operation group, brain TNF-alpha content at 6 h of focal cerebral ischemic ischemia in the model group started to increase and reached to the high peak at 12 h, but in both the Tongmai Huoxue Yin group and the Nimodipine group decreased in varying degrees, with the most obviously decreased at 24 h of ischemia.Tongmai Huoxue Yin could significantly decrease TNF-alpha content in the brain tissue.
  
  
  CONCLUSIONS
  Tongmai Huoxue Yin has a protective action on acute cerebral ischemia injury in the rat.
authors: The Third Hospital Affiliated to Henan University of Traditional Chinese Medicine, Zhengzhou, Henan 450008, China.
authors: Shu-Ke
authors: SK
authors: Cui
authors: Yan
authors: Y
authors: Sun
authors: Fang
authors: F
authors: Cheng
authors: Cheng-Cang
authors: CC
authors: Liu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D008297:Male; D018696:Neuroprotective Agents / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D014409:Tumor Necrosis Factor-alpha