title: Phosphoproteome analysis of Streptomyces development reveals extensive protein phosphorylation accompanying bacterial differentiation.
abstract: Streptomycetes are bacterial species that undergo a complex developmental cycle that includes programmed cell death (PCD) events and sporulation. They are widely used in biotechnology because they produce most clinically relevant secondary metabolites. Although  Streptomyces coelicolor  is one of the bacteria encoding the largest number of eukaryotic type kinases, the biological role of protein phosphorylation in this bacterium has not been extensively studied before. In this issue, the variations of the phosphoproteome of S. coelicolor were characterized. Most distinct Ser/Thr/Tyr phosphorylation events were detected during the presporulation and sporulation stages (80%). Most of these phosphorylations were not reported before in Streptomyces, and included sporulation factors, transcriptional regulators, protein kinases and other regulatory proteins. Several of the identified phosphorylated proteins, FtsZ, DivIVA, and FtsH2, were previously demonstrated to be involved in the sporulation process. We thus established for the first time the widespread occurrence and dynamic features of Ser/Thr/Tyr protein phosphorylation in a bacteria species and also revealed a previously unrecognized phosphorylation motif "x(pT)xEx".
authors: Area de Microbiologia, Departamento de Biologia Funcional and IUBA, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain. mantecaangel@uniovi.es
authors: Angel
authors: A
authors: Manteca
authors: Juanying
authors: J
authors: Ye
authors: Jesús
authors: J
authors: Sánchez
authors: Ole Nørregaard
authors: ON
authors: Jensen
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry*; D019295:Computational Biology; D005590:Fractional Precipitation / Q000379:methods; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D010766:Phosphorylation; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000145:classification; D050436:Regulatory Elements, Transcriptional; D012694:Serine / Q000737:chemistry; D012984:Software; D013171:Spores, Bacterial / Q000737:chemistry / Q000254:growth & development; D048372:Streptomyces coelicolor / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D013912:Threonine / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D010750:Phosphoproteins; D013912:Threonine; D014443:Tyrosine; D012694:Serine; D011494:Protein Kinases