title: [Determination of tetrahydropulmatine content in treatment of winter's diseases in summer path by HPLC].
abstract: OBJECTIVE
  To establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter's diseases in summer path.
  
  
  METHODS
  The analysis was performed on the Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with methanol and 0.1% phosphoric acid solution (adjusted pH to 6.28 with triethylamine) as mobile phase at 63:37. The flow rate was 1.0 mL x min(-1) and the UV detection wavelength was 280 nm.
  
  
  RESULTS
  The linear range of tetrahydropulmatine was obtained from the range of 2.08 - 49.92 mg x L(-1) (r = 0.999 8). The average recovery was 97.85% with RSD 2.49%.
  
  
  CONCLUSIONS
  This method is simple, reliable and can be used for the determination of tetrahydropulmatine in this preparation.
authors: Leqing City Hospital of Traditional Chinese Medicine, Wenzhou 325600, China. minleg@126.com
authors: Jiahao
authors: J
authors: Zheng
authors: Xiaohui
authors: X
authors: Li
authors: Shuhua
authors: S
authors: Zhou
authors: Qiangrong
authors: Q
authors: Zhang
authors: Kan
authors: K
authors: Hu
authors: Jinlai
authors: J
authors: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal