title: Halowax 1051 affects steroidogenesis, 17β-hydroxysteroid dehydrogenase (17β-HSD) and cytochrome P450arom (CYP19) activity, and protein expression in porcine ovarian follicles.
abstract: We evaluated the effect of Halowax 1051 on ovarian follicular testosterone (T) and estradiol (E2) secretion determined by EIA and on the expression of 17β-HSD and CYP19 analyzed by Western blot. 17β-HSD and CYP19 activity were determined indirectly by measuring the conversion of androstenedione (A4) to T and T to E2, respectively. Additionally, CYP19 activity by dibenzylfluorescein assay. Follicles were exposed to 1-1000pg/ml of Halowax 1051 for 24, 48 or 72h. It was found that Halowax 1051, in all doses used, increased basal T secretion concomitant with a decrease in basal E2 secretion. Inhibitory action on 17β-HSD and stimulatory action on CYP19 (activity and protein expression) under the influence of 1pg/ml, and opposite effect under the influence of 10-1000pg/ml was noted. In conclusion, we suggest non-linear dose-response effect of Halowax 1051 on steroidogenesis and steroidogenic enzymes activity and protein expression.
authors: Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Gronostajowa 9, Kraków, Poland. ewa.gregoraszczuk@uj.edu.pl
authors: Ewa Łucja
authors: EŁ
authors: Gregoraszczuk
authors: Justyna
authors: J
authors: Jerzak
authors: Agnieszka
authors: A
authors: Rak-Mardyła
authors: Jerzy
authors: J
authors: Falandysz
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004958:Estradiol / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated / Q000008:administration & dosage / Q000633:toxicity*; D009281:Naphthalenes / Q000008:administration & dosage / Q000633:toxicity*; D006080:Ovarian Follicle / Q000201:enzymology / Q000378:metabolism*; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine; D013739:Testosterone / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D006843:Hydrocarbons, Chlorinated; D009281:Naphthalenes; D013256:Steroids; C571106:halowax 1051; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D001141:Aromatase