title: Construction and activity of a synthetic basement membrane with active laminin peptides and polysaccharides.
abstract: Cell-adhesive peptides derived from extracellular matrix (ECM) proteins are potential candidates for incorporating cell-binding activities into materials for tissue engineering. We have identified a number of cell adhesive peptides from laminins, which are major components of basement membrane ECM. Our goal is the development of synthetic basement membranes using the peptides on scaffolds. We review peptide–polysaccharide complexes, which were prepared by conjugation of the peptides to chitosan and alginate, and the biological activities of the resulting matrices. The peptide–polysaccharide matrices can also be used as a biomaterial for cell transplantation. These studies suggest that the peptide–polysaccharide complexes have the potential to mimic the multifunctional basement membrane and may be useful for tissue engineering.
authors: Laboratory of Clinical Biochemistry, School of Pharmacy Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors: Yuji
authors: Y
authors: Yamada
authors: Kentaro
authors: K
authors: Hozumi
authors: Motoyoshi
authors: M
authors: Nomizu
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism*; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D007797:Laminin; D011134:Polysaccharides; D020723:Glucuronic Acid; D048271:Chitosan