title: Evaluation of wedged arterial injection as a new technique for delivery of experimental therapeutic substances into the porcine pancreas.
abstract: OBJECTIVE
  To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs.
  
  
  METHODS
  Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used.
  
  
  RESULTS
  Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal.
  
  
  CONCLUSIONS
  WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model.
authors: Anatomy and Embryology, Veterinary Faculty, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain. latorre@um.es
authors: Rafael
authors: R
authors: Latorre
authors: Wendy
authors: W
authors: Hernández
authors: Fei
authors: F
authors: Sun
authors: Francisco
authors: F
authors: Sánchez-Margallo
authors: Francisco
authors: F
authors: Gil
authors: Octavio
authors: O
authors: López-Albors
authors: Jose M
authors: JM
authors: Vázquez
authors: Jesús
authors: J
authors: Usón
mesh_terms: D000792:Angiography; D000818:Animals; D057785:Catheters; D002467:Cell Nucleus / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000628:therapy; D016503:Drug Delivery Systems / Q000009:adverse effects; D005240:Feasibility Studies; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics; D018014:Gene Transfer Techniques; D007269:Injections, Intra-Arterial; D050417:Insulin-Secreting Cells / Q000637:transplantation; D008297:Male; D010179:Pancreas / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D011897:Random Allocation; D012039:Regional Blood Flow; D034421:Sus scrofa; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations