title: The effects of hippocampal opioidergic and septal GABAergic system interactions on anxiety-like behavior in rats.
abstract: OBJECTIVE
  Previous studies have shown that the septum and hippocampus are connected and play a key role in anxiety-related behavior. In the present study, the effects of the interactions between the opioidergic system in the dorsal hippocampus (CA1) and the GABAergic system in the medial septum on anxiety in male Wistar rats were investigated.
  
  
  METHODS
  An elevated plus maze was used to study the effects of anxiogenic and anxiolytic drugs in rodents. Male Wistar rats were used for this test. We injected morphine (2.5, 5.0, and 7.5 μg/rat) into the CA1 and GABA(A) agonist muscimol (2.5, 5.0, and 10.0 ng/rat) into the intra-medial septum (MS). Conversely, we detected the microinjection of bicuculline, a GABA(A) antagonist (10, 20, and 30 ng/rat), into the MS. OAT%, OAE% and locomotor activity were determined by this behavioral test.
  
  
  RESULTS
  We found that the simultaneous administration of intra-CA1 morphine (2.5 μg/rat) and intra-MS muscimol (2.5 ng/rat) increased the magnitude of anxiolysis. On the other hand, simultaneous administration of intra-CA1 morphine (7.5 μg/rat) and intra-MS bicuculline decreased the anxiolytic effect.
  
  
  CONCLUSIONS
  In conclusion, our data suggest that the opioidergic system in the CA1 is involved in anxiety-related behavior and influences the GABAergic system within the MS. In addition, we observed that the interactions between the opioidergic and GABAergic systems within the septohippocampus increased anxiety-related behavior compared to stimulation of these receptor systems independently.
authors: Dept. of Biology, Tarbiat Moalem University, Tehran, Iran. as_habi@yahoo.com
authors: Ghorbangol
authors: G
authors: Ashabi
authors: Shahrbanoo
authors: S
authors: Oryan
authors: Ramesh
authors: R
authors: Ahmadi
authors: Farhad
authors: F
authors: Valizadegan
mesh_terms: D000818:Animals; D001007:Anxiety / Q000523:psychology*; D001522:Behavior, Animal / Q000502:physiology*; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology; D056547:CA1 Region, Hippocampal / Q000502:physiology; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018756:GABA Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D008845:Microinjections; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D009294:Narcotics / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000502:physiology*; D017450:Receptors, Opioid, mu / Q000502:physiology*; D020665:Septum of Brain / Q000502:physiology*
chemical_list: D018755:GABA Agonists; D018756:GABA Antagonists; D009294:Narcotics; D018079:Receptors, GABA; D017450:Receptors, Opioid, mu; D009118:Muscimol; D009020:Morphine; D001640:Bicuculline