title: Halioglobus japonicus gen. nov., sp. nov. and Halioglobus pacificus sp. nov., members of the class Gammaproteobacteria isolated from seawater.
abstract: Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36(T) and S1-72(T), were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2% NaCl, and grew at 15-30 °C, with optimum growth at 20-25 °C. Genomic DNA G+C contents of strains S1-36(T) and S1-72(T) were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were C(16:1)ω7c, C(18:1)ω7c and C(17:1)ω8c. Analyses of 16S rRNA gene sequences revealed that strains S1-36(T) and S1-72(T) were related to each other (96.1% sequence similarity) and both strains showed 92.3-94.7% sequence similarity with members of the genus Haliea. On the basis of phenotypic and phylogenetic features, strains S1-36(T) and S1-72(T) should be classified as representatives of two novel species in a new genus, Halioglobus gen. nov., within the class Gammaproteobacteria. The names proposed are Halioglobus japonicus sp. nov., the type species of the genus, with S1-36(T) ( = NBRC 107739(T) = KCTC 23429(T)) as type strain, and Halioglobus pacificus sp. nov., with S1-72(T) ( = NBRC 107742(T) = KCTC 23430(T)) as type strain.
authors: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
authors: Sanghwa
authors: S
authors: Park
authors: Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
authors: Susumu
authors: S
authors: Yoshizawa
authors: Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
authors: Kentaro
authors: K
authors: Inomata
authors: Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
authors: Kazuhiro
authors: K
authors: Kogure
authors: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
authors: Akira
authors: A
authors: Yokota
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D052836:Heterotrophic Processes; D007564:Japan; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S