title: Establishment of a human CEACAM1 transgenic mouse model for the study of gonococcal infections.
abstract: Neisseria gonorrhoeae is the causative microorganism for the sexually transmitted disease (STD) gonorrhea and humans are its only natural host. An animal model would be a useful tool for gonorrhea research, therefore we developed the hCEACAM1 transgenic mice, using an eukaryotic expression vector, pCDPCAM1-GI. This construct was microinjected into the zygotes of C57BL/6 mice and 22 F0 generation transgenic mice were obtained. Four (lines 50, 53, 54, and 59) of the F0 generation were found to carry the transgene by PCR and sequence analysis, respectively. Western blotting and Fluorescence-Activated Cell Sorting Analysis demonstrated that hCEACAM1 was expressed on the cell membrane of various tissues in the line 53 transgenic mouse. To initiate the disease in the animal model, the F2 or F3 transgenic mice were inoculated with N. gonorrhoeae intravaginally. Compared with normal mice, N. gonorrhoeae can successfully infect and cause inflammation in the transgenic mice. These data suggested the feasibility of using hCEACAM1 transgenic mice as an animal model for gonococcal infections.
authors: Department of Pathogen Biology and Immunology, Yangzhou University School of Medicine, Yangzhou, China. gcli@yzu.edu.cn
authors: Guocai
authors: G
authors: Li
authors: Hongmei
authors: H
authors: Jiao
authors: Hua
authors: H
authors: Yan
authors: Jinsong
authors: J
authors: Wang
authors: Xiaohong
authors: X
authors: Wang
authors: Mingchun
authors: M
authors: Ji
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D006069:Gonorrhea / Q000382:microbiology* / Q000473:pathology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D009344:Neisseria gonorrhoeae / Q000472:pathogenicity*
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules