title: IRAK-M in macrophages around septically and aseptically loosened hip implants.
abstract: The most common long-term complication of joint arthroplasty is loosening, which is mediated by chronic inflammatory cytokines produced by macrophages stimulated by implant-derived debris and eventually bacterial components adherent to such debris. In this study, antiinflammatory interleukin-1 receptor-associated kinase-M (IRAK-M) was studied in macrophages in interface membranes in vivo using immunohistochemical staining and in titanium particle-stimulated macrophages in vitro using reverse transcriptase-polymerase chain reaction. Results show that the interface membranes of septically and aseptically loosened prosthesis express more IRAK-M protein than control membranes from osteoarthritic patient and that IRAK-M mRNA-levels increase upon particle stimulation. These findings suggest that, the upregulation of IRAK-M in macrophages is involved in the local immunosuppression around implants, and may contribute to septic and aseptic implant loosening.
authors: Department of Joint Surgery, Huangpu Joint Centre, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 518000, China.
authors: Yangchun
authors: Y
authors: Zhang
authors: Changhe
authors: C
authors: Hou
authors: Shiming
authors: S
authors: Yu
authors: Jianhong
authors: J
authors: Xiao
authors: Ziji
authors: Z
authors: Zhang
authors: Qiyi
authors: Q
authors: Zhai
authors: Jianwei
authors: J
authors: Chen
authors: Ziqing
authors: Z
authors: Li
authors: Xibao
authors: X
authors: Zhang
authors: Matti
authors: M
authors: Lehto
authors: Yrjö T
authors: YT
authors: Konttinen
authors: Puyi
authors: P
authors: Sheng
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D019644:Arthroplasty, Replacement, Hip; D001843:Bone Cements / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006622:Hip Prosthesis / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008875:Middle Aged; D011475:Prosthesis Failure; D016459:Prosthesis-Related Infections / Q000150:complications / Q000201:enzymology* / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018805:Sepsis / Q000201:enzymology* / Q000209:etiology* / Q000473:pathology; D014025:Titanium / Q000494:pharmacology
chemical_list: D001843:Bone Cements; D012333:RNA, Messenger; D014025:Titanium; D053592:Interleukin-1 Receptor-Associated Kinases