title: Human intestinal epithelial cells are susceptible to influenza virus subtype H9N2.
abstract: Avian influenza viruses (AIV) replicate efficiently in guts of birds, and virus shedding is critical to viral transmission among birds and from birds to other species. In this study, we showed that an H9N2 viral strain, isolated from a human patient, caused typical influenza-like signs and illness including loss of body weight in Balb/c mice, and that viral RNA could be detected in intestinal tissues. We demonstrated that human intestinal epithelial cell line HT-29 was susceptible to the virus, and the infected cells went apoptotic at the early stage post infection. Compared to a pandemic (H1N1) 2009 influenza isolate, we found that the human H9N2 virus induced more severe apoptotic and stronger innate immune responses. Both extrinsic and intrinsic apoptotic pathways were activated in human intestinal epithelial cells, and the levels of FasL and TNF-α were induced up to hundreds-fold in response to the H9N2 infection. Interestingly, Bcl-2 family member Bid was cleaved during the course of infection, and the truncated Bid (tBid) appeared to play a role in the initiation of the intrinsic apoptosis with increased release of cytochrome c in cytosol. As for pro-inflammatory responses in H9N2-infected intestinal epithelial cells, RANTES and IP10 were induced significantly and may have played a major role in intestinal pathogenicity. Moreover, TLR-8, MyD88, and MDA-5 were all up-regulated in the infection, critical in the induction of IFN-β and host innate immunity against the H9N2 virus. Our findings have demonstrated a unique pattern of host responses in human gut in response to H9N2 subtype influenza viruses, which will broaden our understanding of the pathogenesis of AIV infection in both humans and animals.
authors: The Key Laboratory of Pharmaceutical Biotechnology and Medical School, Nanjing University, Nanjing, China.
authors: Bingqian
authors: B
authors: Qu
authors: Xue
authors: X
authors: Li
authors: Wei
authors: W
authors: Gao
authors: Wenkui
authors: W
authors: Sun
authors: Yu
authors: Y
authors: Jin
authors: Carol J
authors: CJ
authors: Cardona
authors: Zheng
authors: Z
authors: Xing
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D045304:Cytochromes c / Q000633:toxicity; D003600:Cytosol / Q000201:enzymology; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007113:Immunity, Innate; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D007251:Influenza, Human / Q000821:virology*; D007413:Intestinal Mucosa / Q000166:cytology* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology
chemical_list: D045304:Cytochromes c