title: Knocking down the transcript of NF-kappaB modulates the reciprocal regulation of endogenous antioxidants and feeding behavior in phenylpropanolamine-treated rats.
abstract: It has been reported that oxidative stress, antioxidants, and neuropeptide Y (NPY) are involved in regulating the feeding behavior of phenylpropanolamine (PPA), a sympathomimetic drug. This study explored whether transcription factor NF-κB is involved in this effect. Rats were treated daily with PPA for 4 days. Changes in hypothalamic NF-κB, NPY, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels during PPA treatment were assessed and compared. Results showed that NF-κB, SOD, and GPx increased, with a maximal response on Day 2, while the food intake and NPY decreased with the biggest reduction on Day 2 during PPA treatment. To further determine whether NF-κB was involved, intracerebroventricular infusion of antisense oligonucleotide was performed at 1 h before daily PPA in free-moving rats. Cerebral NF-κB knockdown could modify PPA anorexia and the expressions of NPY, SOD, and GPx. It is suggested that hypothalamic NF-κB participates in the reciprocal regulation of NPY and antioxidants, which mediated the appetite-suppressing effect of PPA. Results may further the understanding of the molecular mechanisms of PPA.
authors: Department of Physiology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City 40201, Taiwan, ROC.
authors: Dong-Yih
authors: DY
authors: Kuo
authors: Pei-Ni
authors: PN
authors: Chen
authors: Shu-Chen
authors: SC
authors: Chu
authors: Yih-Shou
authors: YS
authors: Hsieh
mesh_terms: D000818:Animals; D000855:Anorexia / Q000139:chemically induced; D000975:Antioxidants / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000032:analysis; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology*; D009478:Neuropeptide Y / Q000032:analysis / Q000502:physiology; D010665:Phenylpropanolamine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis; D013566:Sympathomimetics / Q000494:pharmacology*; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D004247:DNA; D005979:Glutathione Peroxidase; D016328:NF-kappa B; D009478:Neuropeptide Y; D010665:Phenylpropanolamine; D013482:Superoxide Dismutase; D013566:Sympathomimetics; D000099156:Superoxide Dismutase 2