title: Increased interleukin (IL)-8 and decreased IL-17 production in chronic obstructive pulmonary disease (COPD) provoked by cigarette smoke.
abstract: Recently, involvement of IL-17 in development of COPD has been noticed. Unlike IL-8, the role of IL-17 in COPD remains controversial. In order to further understand mechanisms in cigarette smoke (CS) induced COPD, we investigated IL-17 and IL-8 levels in different stages of COPD patients, and time courses of IL-17 and IL-8 release in CS induced COPD rats. A total of 73 elderly patients with COPD and 31 healthy volunteers were recruited in the study. IL-17 and IL-8 levels in the sputum and plasma were measured, and number of differential cells was counted. A newly developed CS induced rat COPD model was employed to study time courses of IL-17 and IL-8 release and nucleated cell accumulation. The results showed that IL-8 levels in the sputum of severe COPD patients were elevated by 16.5-fold, but IL-17 levels were reduced by 4.8-fold. While IL-8 correlated with neutrophils, IL-17 correlated with monocytes and lymphocytes. Similarly, level of IL-8 was increased, but IL-17 was decreased in the bronchoalveolar lavage fluid (BALF) of CS rats. Time course study showed that increased IL-8 production in the BALF initiated at 6 weeks, but decreased IL-17 production started at 10 weeks following CS exposure. In conclusion, increased IL-8 level in COPD patients appears mainly secreted from neutrophils and macrophages, whereas decreased IL-17 level seems resulted from reduced number of monocytes or damaged epithelial cells. Increased IL-8 (a proinflammatory cytokine) secretion and decreased IL-17 (a protective cytokine of airways) release can both contribute to development of COPD.
authors: The General Hospital of PLA, Beijing 100853, China.
authors: Xiaojun
authors: X
authors: Zhang
authors: Honggao
authors: H
authors: Zheng
authors: Huiyun
authors: H
authors: Zhang
authors: Wenjing
authors: W
authors: Ma
authors: Fang
authors: F
authors: Wang
authors: Changting
authors: C
authors: Liu
authors: Shaoheng
authors: S
authors: He
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001772:Blood Cell Count; D001992:Bronchoalveolar Lavage Fluid; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000150:complications / Q000473:pathology; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000097:blood; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood; D008168:Lung / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D010343:Patient Admission; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000139:chemically induced / Q000150:complications; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*; D013183:Sputum / Q000378:metabolism; D013997:Time Factors
chemical_list: D020381:Interleukin-17; D016209:Interleukin-8