title: A target analogue imprinted polymer for the recognition of antiplatelet active ingredients in Radix Salviae Miltiorrhizae by LC/MS/MS.
abstract: The purpose of this study was to prepare a propyl gallate (PrG) molecular imprinted polymer as a cartridge stuffing material to isolate antiplatelet active ingredients. A macroporous polymer was synthesized utilizing ethylene glycol dimethacrylate (EDMA) as the crosslinking agent, PrG as the template molecule and 4-vinylpyridine (4-Vpy) as the functional monomer. Subsequently, PrG was removed by washing with methanol-glacial acetic acid (9:1, v/v). The molecular imprinted polymer recognized an active ingredient, protocatechuic acid, from a crude extract of the Chinese herbal medicine, Radix Salviae Miltiorrhizae (Danshen), using an on-line column switching solid phase extraction process. Pharmacological experiments showed that protocatechuic acid inhibits arachidonic acid (10 mg/kg) induced aggregation of rat platelets in vivo. This study provides an example of an application of separation-analysis technique for screening potentially bioactive compounds.
authors: School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
authors: Meixia
authors: M
authors: Huang
authors: Wensheng
authors: W
authors: Pang
authors: Jing
authors: J
authors: Zhang
authors: Siding
authors: S
authors: Lin
authors: Juan
authors: J
authors: Hu
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects; D002853:Chromatography, Liquid / Q000379:methods; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods; D010618:Phenanthrolines / Q000737:chemistry* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011435:Propyl Gallate / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D008689:Methacrylates; D010618:Phenanthrolines; D010975:Platelet Aggregation Inhibitors; D011108:Polymers; D011725:Pyridines; C028972:dan-shen root extract; D016718:Arachidonic Acid; C009091:protocatechuic acid; C004919:ethylene dimethacrylate; D011435:Propyl Gallate; C029351:4-vinylpyridine