title: Coupling of permeabilized cells of Gluconobacter oxydans and Ralstonia eutropha for asymmetric ketone reduction using H2 as reductant.
abstract: A combined two-cell reaction system containing Gluconobacter oxydans and Ralstonia eutropha was evaluated with regard to asymmetric ketone reduction using H(2) as the reductant. Whole cells permeabilized by EDTA/toluene were used, and synthesis was performed in a biphasic aqueous/organic reaction medium. The two-cell system was compared with a system in which G. oxydans alone was used for both ketone reduction and cofactor regeneration, using an alcohol as co-substrate. The two-cell system exhibited almost twice the initial reaction rate of the single-cell system, a higher yield (75% vs. 48%) but slightly lower enantiomeric purity (93% vs. 98%) of the product (S)-2-octanol. The permeabilized R. eutropha cells are worth evaluating for byproduct-free NADH regeneration in combination with other whole cell catalysts.
authors: Department of Biotechnology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors: Fabian
authors: F
authors: Rundbäck
authors: Milica
authors: M
authors: Fidanoska
authors: Patrick
authors: P
authors: Adlercreutz
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D020118:Cupriavidus necator / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000378:metabolism*; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010539:Permeability; D019163:Reducing Agents / Q000737:chemistry / Q000378:metabolism
chemical_list: D000438:Alcohols; D007659:Ketones; D019163:Reducing Agents; D009243:NAD; D006859:Hydrogen; D006864:Hydrogenase