title: Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon).
abstract: Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar), between 1994 and 2002. The bacteria were Gram-negative, rod-shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow in the presence of 0.5-8.0% NaCl (optimum 3.0-6.0%) and at 10-37 °C (optimum 25-30 °C). On the basis of 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) using five loci (2443 bp; gyrB, pyrH, ftsZ, mreB and gapA), the closest phylogenetic neighbours of strain TCFB 0772(T) were the type strains of Vibrio communis (99.8 and 94.6 % similarity, respectively), Vibrio owensii (99.8 and 94.1%), Vibrio natriegens (99.4 and 88.8%), Vibrio parahaemolyticus (99.4 and 90.3%), Vibrio rotiferianus (99.2 and 94.4%), Vibrio alginolyticus (99.1 and 89.3%) and Vibrio campbellii (99.1 and 92.3%). DNA-DNA hybridization confirmed that the six isolates constitute a unique taxon that is distinct from other known species of Vibrio. In addition, this taxon can be readily differentiated phenotypically from other Vibrio species. The six isolates therefore represent a novel species, for which the name Vibrio jasicida sp. nov. is proposed; the novel species is represented by the type strain TCFB 0772(T) ( = JCM 16453(T)  = LMG 25398(T)) (DNA G+C content 45.9 mol%) and reference strains TCFB 1977 ( = JCM 16454) and TCFB 1000 ( = JCM 16455).
authors: Atmosphere and Ocean Research Institute, the University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
authors: Susumu
authors: S
authors: Yoshizawa
authors: Laboratory of Microbiology, Faculty of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan.
authors: Yasuhiro
authors: Y
authors: Tsuruya
authors: Laboratory of Microbiology, Faculty of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan.
authors: Youhei
authors: Y
authors: Fukui
authors: Laboratory of Microbiology, Faculty of Fisheries Science, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan.
authors: Tomoo
authors: T
authors: Sawabe
authors: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
authors: Akira
authors: A
authors: Yokota
authors: Atmosphere and Ocean Research Institute, the University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
authors: Kazuhiro
authors: K
authors: Kogure
authors: Fish Health Unit, Department of Primary Industries, Parks, Water & Environment, PO Box 46, Kings Meadows, Launceston, Tasmania 7249, Australia.
authors: Melissa
authors: M
authors: Higgins
authors: Fish Health Unit, Department of Primary Industries, Parks, Water & Environment, PO Box 46, Kings Meadows, Launceston, Tasmania 7249, Australia.
authors: Jeremy
authors: J
authors: Carson
authors: Department of Genetics, Center of Health Sciences, Federal University of Rio de Janeiro, Ilha do Fundão, Caixa Postal 68011, CEP 21944-970, RJ, Brazil.
authors: Fabiano L
authors: FL
authors: Thompson
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D049851:Gastropoda / Q000382:microbiology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D033521:Palinuridae / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020317:Salmo salar / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S