title: Quantitative and phenotypic analysis of bone marrow-derived cells in the intact and inflamed central nervous system.
abstract: Bone marrow has been proposed as a possible source of cells capable of replacing injured neural cells in diseases such as Multiple Sclerosis (MS). Previous studies have reported conflicting results regarding the transformation of bone marrow cells into neural cells in vivo. This study is a detailed analysis of the fate of bone marrow derived cells (BMDC) in the CNS of C57Bl/6 mice with and without experimental autoimmune encephalomyelitis using flow cytometry to identify GFP-labeled BMDC that lacked the pan-hematopoietic marker, CD45 and co-expressed neural markers polysialic acid-neural cell adhesion molecule or A2B5. A small number of BMDC displaying neural markers and lacking CD45 expression was identified within both the non-inflamed and inflamed CNS. However, the majority of BMDC exhibited a hematopoietic phenotype.
authors: Monash Immunology and Stem Cell Laboratories; Monash University; Clayton, VIC Australia.
authors: Martin A
authors: MA
authors: Short
authors: Naomi
authors: N
authors: Campanale
authors: Sara
authors: S
authors: Litwak
authors: Claude C A
authors: CC
authors: Bernard
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology*; D016026:Bone Marrow Transplantation; D002490:Central Nervous System / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology*; D005732:Gangliosides / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism; D010641:Phenotype
chemical_list: D015415:Biomarkers; D005732:Gangliosides; C062875:ganglioside A2B5; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens