title: Role of TLR4 and TCR or BCR against baicalin-induced responses in T and B cells.
abstract: Baicalin (BA), a flavonoid compound isolated from Scutellaria baicalensis, has been shown to possess a number of pharmacological effects including antiviral, anti-inflammatory, antioxidant and immune regulation. Here, we examined its effects on human T and B cells proliferation by MTT assay and found that BA stimulated T and B cells proliferation, independently and cooperatively with Con-A (T cells) or LPS (B cells). Then, we analyzed the effects of BA treatment on the mRNA expression of Toll-like receptors (TLRs), IL-2, IFN-γ and IL-12 in T and B cells by real-time RT-PCR and attempted to observe whether blocking TLR4 had influence on mRNA expression. We found that BA treatment significantly up-regulated TLR3, 7, 8 and 9 mRNA expressions in T and B cells, IL-2 and IFN-γ in T cells and IL-12 in B cells. The increased mRNA expressions were suppressed after blocking TLR4. We further analyzed the effects of BA treatment on TCR vβ and CD79 mRNA expression levels in T and B cells and explored whether blocking TCR (αβ) or BCR mIgM F(ab')(2) had an influence on mRNA expression. We found that BA treatment significantly improved TCR vβ and CD79 mRNA expression in T and B cells, respectively, and the improvements were all inhibited after blocking TCR (αβ) or BCR mIgM F(ab')(2). Our results suggested that BA participates in innate and adaptive immune regulation by up-regulating the mRNA expression of TLRs (3, 7, 8 and 9), IL-2, IFN-γ and IL-12 in T and B cells, which is mediated by TLR4, and by improving the mRNA expression of TCR vβ and CD79, which is mediated by TCR (αβ) and BCR mIgM, respectively. Therefore, TLR4, TCR (αβ) and BCR mIgM are all the immune receptors for BA on T and B cells.
authors: Department of Immunology, medical college of NanChang University, Ba-yi road, No. 461. NanChang city 330006, JiangXi province, China.
authors: Shu-Qi
authors: SQ
authors: Gong
authors: Wu
authors: W
authors: Sun
authors: Min
authors: M
authors: Wang
authors: Ying-Yuan
authors: YY
authors: Fu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000096:biosynthesis / Q000276:immunology; D015854:Up-Regulation
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D005419:Flavonoids; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D003208:Concanavalin A; C038044:baicalin