title: An Escherichia coli-based bioengineering strategy to study streptolysin S biosynthesis.
abstract: Group A Streptococcus pyogenes (GAS) is a leading human pathogen that produces a powerful cytolytic bacteriocin known as streptolysin S (SLS). We have developed a bioengineering strategy to successfully reconstitute SLS activity using heterologous expression in laboratory strains of Escherichia coli. Our E. coli-based heterologous expression system will allow more detailed studies into the biosynthesis of other bacteriocin compounds and the production of these natural products in much greater yield.
authors: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
authors: Andrew L
authors: AL
authors: Markley
authors: Emily R
authors: ER
authors: Jensen
authors: Shaun W
authors: SW
authors: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000633:toxicity; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006461:Hemolysis / Q000187:drug effects; D005810:Multigene Family / Q000235:genetics; D012756:Sheep; D013301:Streptolysins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000633:toxicity
chemical_list: D001426:Bacterial Proteins; D013301:Streptolysins; C026050:streptolysin S