title: Profiling hydroxycinnamoyl-coenzyme A thioesters: unlocking the back door of phenylpropanoid metabolism.
abstract: In plants, 20 to 30% of photosynthetically fixed carbon is directed toward lignin and other phenylpropanoid compounds for which hydroxycinnamoyl-coenzyme A (CoA) esters are key intermediates. CoA thioesters, ubiquitous metabolites found in all living cells (often at trace levels), have traditionally been challenging to measure. Here we report a hydrophilic interaction liquid chromatography (HILIC) method, coupled with tandem mass spectrometry (MS/MS), that allows simultaneous sensitive quantification of previously undetectable hydroxycinnamoyl-CoA esters and an extended range of acyl-CoAs from plant tissues. This method provides rapid liquid chromatography (LC) analysis (10 min/sample) and the ability for qualitative assessment of acyl-CoAs by MS/MS precursor ion scanning.
authors: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
authors: Anthony V
authors: AV
authors: Qualley
authors: Bruce R
authors: BR
authors: Cooper
authors: Natalia
authors: N
authors: Dudareva
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry* / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000737:chemistry* / Q000378:metabolism*; D004952:Esters; D032306:Petunia / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C403206:4-hydroxycinnamoyl-CoA; D000214:Acyl Coenzyme A; D003373:Coumaric Acids; D004952:Esters