title: 125I-labeled gold nanorods for targeted imaging of inflammation.
abstract: For better examination of inflammation, we designed inflammation-targeted nuclear and optical dual-modality contrast agents prepared by I-125 radiolabeling of gold nanorods (GdNRs) conjugated with anti-intercellular adhesion molecule 1 (ICAM-1) antibody. The bioactivity and specific binding of the PEGylated (125)I-ICAM-GdNR conjugates to the ICAM-1 was validated through ELISA testing. Inflammation-targeted imaging was then conducted on an adjuvant-induced arthritic rat model which demonstrated an elevation of ICAM-1 level in the affected ankle joints. Facilitated by the I-125 radioisotope and the whole-body imaging via the Gamma camera, the time-dependent distribution of the systemically injected agent as well as the uptake of the agent in the inflammatory articular tissues could be examined conveniently and quantitatively. The success in targeted delivery of gold nanoparticles to inflammatory tissue enables both nuclear and optical imaging of inflammation at molecular or cellular level. Other than diagnosis, radiolabeled gold nanoparticles also hold promise for targeted therapy of a variety of disorders.
authors: Department of Radiology, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors: Xia
authors: X
authors: Shao
authors: Huanan
authors: H
authors: Zhang
authors: Justin R
authors: JR
authors: Rajian
authors: David L
authors: DL
authors: Chamberland
authors: Phillip S
authors: PS
authors: Sherman
authors: Carole A
authors: CA
authors: Quesada
authors: Alisa E
authors: AE
authors: Koch
authors: Nicholas A
authors: NA
authors: Kotov
authors: Xueding
authors: X
authors: Wang
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000000981:diagnostic imaging; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D001168:Arthritis / Q000000981:diagnostic imaging / Q000378:metabolism / Q000382:microbiology; D001692:Biological Transport; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D005786:Gene Expression Regulation; D006046:Gold / Q000737:chemistry*; D007249:Inflammation / Q000000981:diagnostic imaging / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D009161:Mycobacterium / Q000502:physiology; D058726:Nanoconjugates / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D011877:Radionuclide Imaging / Q000379:methods*; D051381:Rats
chemical_list: D000906:Antibodies; D003287:Contrast Media; D016207:Cytokines; D007457:Iodine Radioisotopes; D058726:Nanoconjugates; D018799:Intercellular Adhesion Molecule-1; D006046:Gold