title: Development and characterization of EST-SSR markers in Paeonia suffruticosa (Paeoniaceae).
abstract: OBJECTIVE
  Paeonia suffruticosa is one of the most famous flowering species in China. Ten simple sequence repeat (SSR) markers were developed and characterized from the expressed sequence tag (EST) database of tree peonies to be applied in genetic diversity studies.
  
  
  RESULTS
  A total of 10 novel EST-SSR loci were developed and characterized when tested on two populations of 45 tree peony individuals from China. The number of alleles per locus ranged from one to four and the expected heterozygosity ranged from 0.2087 to 0.6581. Select loci also amplified successfully in three other Paeonia species (P. ostii, P. rockii, P. jishanensis).
  
  
  CONCLUSIONS
  These markers will facilitate analysis of genetic diversity, population differentiation, and marker-assisted breeding of Paeoniaceae.
authors: College of Agriculture, Henan University of Science and Technology, Luoyang 471003, People's Republic of China.
authors: Xiao-Gai
authors: XG
authors: Hou
authors: Da-Long
authors: DL
authors: Guo
authors: Juan
authors: J
authors: Wang
mesh_terms: D000483:Alleles; D001947:Breeding; D002681:China; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats; D029596:Paeonia / Q000235:genetics*; D013045:Species Specificity; D014197:Trees / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers