title: Determination of HX0969w in rabbit whole blood by liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.
abstract: HX0969w is a novel carboxylate ester prodrug of propofol. After intravenous administration of HX0969w, the compound is rapidly hydrolyzed to its active metabolite propofol. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with electrospray ionization has been developed for determination of HX0969w in rabbit whole blood. Protein precipitation with methanol was used for sample preparation and 7-hydroxycoumarin served as internal standard. The standard curve ranged from 50.1 to 15,030 ng mL(-1). The lower limit of quantification (LLOQ) for HX0969w was 50.1 ng mL(-1). The intra- and inter-day accuracies were within ± 10% and precisions were below 5.52%. The method was successfully applied to samples from a rabbit pharmacokinetic study.
authors: West China Hospital, Sichuan University, Chengdu, China.
authors: Wang
authors: W
authors: Yin
authors: Yu
authors: Y
authors: Wang
authors: Jun
authors: J
authors: Yang
authors: Yi
authors: Y
authors: Kang
authors: Wen-Sheng
authors: WS
authors: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000097:blood*; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C569054:2,6-diisopropylphenyl-4-hydroxybutanote disodium phosphate; D009943:Organophosphorus Compounds