title: Caged glucosamine-6-phosphate for the light-control of riboswitch activity.
abstract: We have synthesized a light-activatable ("caged") derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3'-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro.
authors: University of Bonn, Life and Medical Sciences, c/o Kekulé-Institute for Organic Chemistry and Biochemistry, Bonn, Germany.
authors: Bernhard
authors: B
authors: Wulffen
authors: Maximilian Clemens Rudolf
authors: MC
authors: Buff
authors: Monika
authors: M
authors: Pofahl
authors: Günter
authors: G
authors: Mayer
authors: Alexander
authors: A
authors: Heckel
mesh_terms: D005944:Glucosamine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D008027:Light; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D058928:Riboswitch
chemical_list: D016337:RNA, Catalytic; D058928:Riboswitch; C001293:glucosamine 6-phosphate; D019298:Glucose-6-Phosphate; D005944:Glucosamine