title: A triple spin-labeling strategy coupled with DEER analysis to detect DNA modifications and enzymatic repair.
abstract: In a spin: Spin-labeled oligonucleotides produced by click chemistry can be studied by EPR, by using a DEER sequence. This was used to test a complex triple-labeling strategy with damaged DNA. Extensive and accurate analysis of DNA structure and enzymatic repair processes were performed after digestion by EndoIV. Modified DNA structures and DNA-protein interactions can now be readily studied.
authors: Institut Nanoscience et Cryogénie, Laboratoire de Chimie Inorganique et Biologique, UMR-E n°3 CEA-UJF, CEA-Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France.
authors: Mélanie
authors: M
authors: Flaender
authors: Giuseppe
authors: G
authors: Sicoli
authors: Samia
authors: S
authors: Aci-Seche
authors: Thomas
authors: T
authors: Reignier
authors: Vincent
authors: V
authors: Maurel
authors: Christine
authors: C
authors: Saint-Pierre
authors: Yves
authors: Y
authors: Boulard
authors: Serge
authors: S
authors: Gambarelli
authors: Didier
authors: D
authors: Gasparutto
mesh_terms: D057930:Click Chemistry; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D004249:DNA Damage; D043223:Deoxyribonuclease IV (Phage T4-Induced) / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry; D013113:Spin Labels
chemical_list: D009841:Oligonucleotides; D013113:Spin Labels; D004247:DNA; D043223:Deoxyribonuclease IV (Phage T4-Induced)