title: Structural insights regarding an insecticidal Talisia esculenta protein and its biotechnological potential for Diatraea saccharalis larval control.
abstract: Talisin is a seed-storage protein from Talisia esculenta that presents lectin-like activities, as well as proteinase-inhibitor properties. The present study aims to provide new in vitro and in silico biochemical information about this protein, shedding some light on its mechanistic inhibitory strategies. A theoretical three-dimensional structure of Talisin bound to trypsin was constructed in order to determine the relative interaction mode. Since the structure of non-competitive inhibition has not been elucidated, Talisin-trypsin docking was carried out using Hex v5.1, since the structure of non-competitive inhibition has not been elucidated. The predicted non-coincidence of the trypsin binding site is completely different from that previously proposed for Kunitz-type inhibitors, which demonstrate a substitution of an Arg(64) for the Glu(64) residue. Data, therefore, provide more information regarding the mechanisms of non-competitive plant proteinase inhibitors. Bioassays with Talisin also presented a strong insecticide effect on the larval development of Diatraea saccharalis, demonstrating LD50 and ED50 of ca. 2.0% and 1.5%, respectively.
authors: LAQUIBIO, ISECENSA, Campos dos Goytacazes-RJ, Brazil.
authors: Maria das Graças M
authors: Md
authors: Freire
authors: Octávio L
authors: OL
authors: Franco
authors: Carlos Eduardo G
authors: CE
authors: Kubo
authors: Ludovico
authors: L
authors: Migliolo
authors: Rodrigo H
authors: RH
authors: Vargas
authors: Caio Fernando Ramalho
authors: CF
authors: de Oliveira
authors: José Roberto P
authors: JR
authors: Parra
authors: Maria Ligia R
authors: ML
authors: Macedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001709:Biotechnology; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D007306:Insecticides / Q000737:chemistry* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D007915:Lepidoptera / Q000187:drug effects*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D014357:Trypsin / Q000378:metabolism
chemical_list: D007306:Insecticides; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D014357:Trypsin