title: Reference intervals for saliva analytes collected by a standardized method in a physically active population.
abstract: OBJECTIVE
  Our aims were to test a liquid-based saliva collection system for total antioxidant status (TAS), uric acid (UA), total protein concentration (TP) and salivary alpha-amylase (SAA) activity; to determine if these analytes in serum and saliva are correlated in a physically active population and to establish reference intervals for these parameters.
  
  
  METHODS
  Participants in this study were 115 physically active males (18-20 years old). Saliva samples were collected using the Saliva Collection System (Greiner Bio-One) immediately before obtaining blood. Biochemical analyses were conducted using an Autolab Boehringer analyzer.
  
  
  RESULTS
  We found a correlation between UA and TP concentrations in serum and saliva samples. The reference intervals for TP and SAA activity in the morning were lower than in the afternoon (p<0.0001). The reference intervals for UA and TAS did not vary with the time of collection.
  
  
  CONCLUSIONS
  The establishment of reference intervals for these saliva constituents increases their diagnostic utility and allows for detection of physiological or pathological states.
authors: Laboratory of Exercise Biochemistry - LABEX, Biochemistry Department, Biology Institute, State University of Campinas, Cidade Universitária Zeferino Vaz, s/n, Barão Geraldo, CEP: 13083-970, CP6109, Campinas, SP, Brazil. lazaroalessandro@yahoo.com.br
authors: Lázaro Alessandro Soares
authors: LA
authors: Nunes
authors: René
authors: R
authors: Brenzikofer
authors: Denise Vaz
authors: DV
authors: Macedo
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D008297:Male; D008889:Military Personnel; D010507:Periodicity; D012016:Reference Values; D012463:Saliva / Q000201:enzymology / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D014527:Uric Acid / Q000378:metabolism*; D055815:Young Adult; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D012471:Salivary Proteins and Peptides; D014527:Uric Acid; D000516:alpha-Amylases