title: A correlative approach at characterizing nanoparticle mobility and interactions after cellular uptake.
abstract: The interactions of nanoparticles with human cells are of large interest in the context of nanomaterial safety. Here, we use live cell imaging and image-based fluorescence correlation methods to determine colocalization of 88 nm and 32 nm silica nanoparticles with endocytotic vesicles derived from the cytoplasmic membrane and lysosomes, as well as to quantify intracellular mobility of internalized particles, in contrast to particle number quantification by counting techniques. In our study, A549 cells are used as a model for human type II alveolar epithelial cells. We present data supporting endocytotic uptake of the particles and subsequent active transport to the perinuclear region. The presence of particles in lamellar bodies is proposed as a potential exocytosis route.
authors: INM - Leibniz Institute for New Materials, Nano Cell Interactions Group, Saarbrücken, Germany.
authors: Christian
authors: C
authors: Schumann
authors: Sabrina
authors: S
authors: Schübbe
authors: Christian
authors: C
authors: Cavelius
authors: Annette
authors: A
authors: Kraegeloh
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008247:Lysosomes / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods; D008954:Models, Biological; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D010957:Plasmids / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology; D012822:Silicon Dioxide / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D014162:Transfection
chemical_list: D012822:Silicon Dioxide