title: Antisense oligonucleotide targeting matrix metalloproteinase-7 (MMP-7) changes the ultrastructure of human A549 lung adenocarcinoma cells.
abstract: Antisense oligonucleotide (ASODN) targeting specific gene can be capable of potently downregulating proliferation and invasion in human cancer cells. However, the underlying mechanisms are less well defined. Here the authors show that matrix metalloproteinase-7 (MMP-7) ASODN changes the ultrastructure of human A549 lung adenocarcinoma cells. Transfection of MMP-7 ASODN significantly lowered the expression of MMP-7 protein in A549 cells. Decreased microvilli, endoplasmic reticulum dilation, swelling of mitochondria, and formation of apoptotic bodies were observed by transmission electron microscope. Collectively, the findings identified the morphological mechanism that MMP-7 ASODN inhibited proliferation and invasion in A549 cells.
authors: Department of Oncology, Wuhan General Hospital of Guangzhou Command PLA, 627 Wuluo Road, Wuhan, P.R. China.
authors: Bo
authors: B
authors: Yang
authors: Jianfei
authors: J
authors: Gao
authors: Zhiguo
authors: Z
authors: Rao
authors: Bicheng
authors: B
authors: Zhang
authors: Wen
authors: W
authors: Ouyang
authors: Chunxu
authors: C
authors: Yang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009361:Neoplasm Invasiveness; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D014162:Transfection
chemical_list: D016376:Oligonucleotides, Antisense; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7