title: Dysregulation of gene expression in the artificial human trisomy cells of chromosome 8 associated with transformed cell phenotypes.
abstract: A change in chromosome number, known as aneuploidy, is a common characteristic of cancer. Aneuploidy disrupts gene expression in human cancer cells and immortalized human epithelial cells, but not in normal human cells. However, the relationship between aneuploidy and cancer remains unclear. To study the effects of aneuploidy in normal human cells, we generated artificial cells of human primary fibroblast having three chromosome 8 (trisomy 8 cells) by using microcell-mediated chromosome transfer technique. In addition to decreased proliferation, the trisomy 8 cells lost contact inhibition and reproliferated after exhibiting senescence-like characteristics that are typical of transformed cells. Furthermore, the trisomy 8 cells exhibited chromosome instability, and the overall gene expression profile based on microarray analyses was significantly different from that of diploid human primary fibroblasts. Our data suggest that aneuploidy, even a single chromosome gain, can be introduced into normal human cells and causes, in some cases, a partial cancer phenotype due to a disruption in overall gene expression.
authors: Laboratory of Radiation Biology, Research Reactor Institute, Kyoto University, Osaka, Japan.
authors: Hisakatsu
authors: H
authors: Nawata
authors: Genro
authors: G
authors: Kashino
authors: Keizo
authors: K
authors: Tano
authors: Kazuhiro
authors: K
authors: Daino
authors: Yoshiya
authors: Y
authors: Shimada
authors: Hiroyuki
authors: H
authors: Kugoh
authors: Mitsuo
authors: M
authors: Oshimura
authors: Masami
authors: M
authors: Watanabe
mesh_terms: D058045:Artificial Cells / Q000378:metabolism*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D043171:Chromosomal Instability / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D003260:Contact Inhibition / Q000235:genetics; D004249:DNA Damage / Q000235:genetics; D004171:Diploidy; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D059467:Transcriptome; D014314:Trisomy / Q000235:genetics* / Q000473:pathology*