title: Assessment of serological evidence for mumps virus infection in vaccinated children.
abstract: It is estimated that at least one-third of mumps virus infections in non-vaccinated individuals are asymptomatic. Little information is available whether this proportion is the same among those vaccinated. We validated a commercial oral fluid mumps IgG-specific Enzyme Immunoassays (EIA) with vaccinated control groups to identify symptomatic and asymptomatic mumps virus infections in vaccinated individuals during a mumps outbreak in The Netherlands. A vaccinated control group was required to define a new cutoff value for the assay, because of the presence of low but significant levels of IgG antibodies in oral fluid as a result of mumps vaccination in the past. With a new cutoff, calculated using receiver operator characteristic analysis, we identified an attack rate of 7-10% compared to 2.7% based on clinical symptoms among vaccinated children. This finding has important implications when studying transmission patterns, strain virulence, as well as mumps vaccine effectiveness to protect from infection rather than disease.
authors: Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands. Sabine-Dittrich@gmx.de
authors: Sabine
authors: S
authors: Dittrich
authors: Susan
authors: S
authors: Hahné
authors: Alies
authors: A
authors: van Lier
authors: Robert
authors: R
authors: Kohl
authors: Hein
authors: H
authors: Boot
authors: Marion
authors: M
authors: Koopmans
authors: Robert
authors: R
authors: van Binnendijk
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D008297:Male; D009107:Mumps / Q000175:diagnosis* / Q000453:epidemiology; D009108:Mumps Vaccine / Q000008:administration & dosage*; D009109:Mumps virus / Q000276:immunology; D009426:Netherlands; D012372:ROC Curve; D012463:Saliva / Q000276:immunology; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D009108:Mumps Vaccine