title: A novel FUT1 allele was identified in a Chinese individual with para-Bombay phenotype.
abstract: BACKGROUND
  The para-Bombay phenotype is characterised by H-deficient or H partially deficient red blood cells (RBCs) in individuals who secrete ABH antigens in their saliva. Samples from an individual whose RBCs had an apparent para-Bombay phenotype and his family members were investigated and a novel FUT1 allele was identified.
  
  
  METHODS
  RBCs' phenotype was characterised by standard serologic technique. Genomic DNA was sequenced with primers that amplified the coding sequence of FUT1 and FUT2, respectively. Routine ABO genotyping analysis was performed. Haplotypes of FUT1 were identified by TOPO cloning sequencing. Recombination expression vectors of FUT1 mutation alleles were constructed and transfected into COS-7 cells. The pα-(1,2)-fucosyltransferase activity of expression protein was determined.
  
  
  RESULTS
  B101/O02 genotype of the proband was correlated with ABH substances in saliva. The proband carried a new FUT1 allele which showed 35C/T, 235G/C and 682A/G heterozygote by directly DNA sequencing. Two haplotypes, 235C and 35T+682G, were identified by TOPO cloning sequencing and COS-7 cells transfected with five recombination vectors including wild-type, 35T, 235C, 682G and 35T+682G alleles were established respectively. The α-(1,2)-fucosyltransferase activities of cell lysates which had transfected with 35T, 235C, 682G and 35T+682G recombination vectors showed 79·45, 16·23, 80·32 and 24·59%, respectively, compared with that of the wild-type FUT1-transfected cell lysates.
  
  
  CONCLUSIONS
  A novel FUT1 allele 235C was identified, which greatly diminished the activity of α-(1,2)-fucosyltransferase.
authors: Blood Center of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China.
authors: X
authors: X
authors: Xu
authors: S
authors: S
authors: Tao
authors: Y
authors: Y
authors: Ying
authors: X
authors: X
authors: Hong
authors: Y
authors: Y
authors: He
authors: F
authors: F
authors: Zhu
authors: H
authors: H
authors: Lv
authors: L
authors: L
authors: Yan
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D004912:Erythrocytes; D005190:Family; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics*; D006801:Humans; D008297:Male; D009154:Mutation; D010641:Phenotype; D012463:Saliva / Q000276:immunology; D017422:Sequence Analysis, DNA; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000017:ABO Blood-Group System; D005647:Fucosyltransferases; D000097631:Galactoside 2-alpha-L-fucosyltransferase