title: Genotoxicity evaluation of dimethoate to experimental mice by micronucleus, chromosome aberration tests, and comet assay.
abstract: Dimethoate (DM) is an organophosphate insecticide with numerous uses on field and agricultural crops and ornamentals. Data concerning DM-acute genotoxicity are controversial and knowledge on its delayed effect is limited. For this reason, we aimed to further explore DM genotoxicity resulting from subchronic intoxication of experimental mice. Thus, DM was administered to mice at doses ranging from 1 to 30 mg/kg body weight for a period of 30 consecutive days. There was a significant increase (P < .05) in the frequency of micronucleated bone marrow cells following DM administration. Furthermore, the chromosome aberration assay revealed a significant increase in the percentage of chromosome abnormalities in a dose-dependent manner. Dimethoate was also found to induce significant DNA damage in mouse bone marrow cells as assessed by the comet assay. Altogether, our results showed that, after a subchronic exposure, DM was a genotoxic compound in experimental mice.
authors: Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, 5019 Monastir, Tunisia. hassen.bacha@fmdm.rnu.tn
authors: Imen
authors: I
authors: Ayed-Boussema
authors: Karima
authors: K
authors: Rjiba
authors: Nourhène
authors: N
authors: Mnasri
authors: Amal
authors: A
authors: Moussa
authors: Hassen
authors: H
authors: Bacha
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002869:Chromosome Aberrations / Q000139:chemically induced; D020552:Comet Assay; D004249:DNA Damage; D004117:Dimethoate / Q000633:toxicity*; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
chemical_list: D007306:Insecticides; D009153:Mutagens; D004117:Dimethoate