title: Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis.
abstract: Increasing the energy density of biomass by engineering the accumulation of triacylglycerols (TAGs) in vegetative tissues is synergistic with efforts to produce biofuels by conversion of lignocellulosic biomass. Typically, TAG accumulates in developing seeds, and little is known about the regulatory mechanisms and control factors preventing oil biosynthesis in vegetative tissues in most plants. Here, we engineered Arabidopsis thaliana to ectopically overproduce the transcription factor WRINKLED1 (WRI1) involved in the regulation of seed oil biosynthesis. Furthermore, we reduced the expression of APS1 encoding a major catalytic isoform of the small subunit of ADP-glucose pyrophosphorylase involved in starch biosynthesis using an RNAi approach. The resulting AGPRNAi-WRI1 lines accumulated less starch and more hexoses. In addition, these lines produced 5.8-fold more oil in vegetative tissues than plants with WRI1 or AGPRNAi alone. Abundant oil droplets were visible in vegetative tissues. TAG molecular species contained long-chain fatty acids, similar to those found in seed oils. In AGPRNAi-WRI1 lines, the relative expression level of sucrose synthase 2 was considerably elevated and correlated with the level of sugars. The relative expression of the genes encoding plastidic proteins involved in de novo fatty acid synthesis, biotin carboxyl carrier protein isoform 2 and acyl carrier protein 1, was also elevated. The relative contribution of TAG compared to starch to the overall energy density increased 9.5-fold in one AGPRNAi-WRI1 transgenic line consistent with altered carbon partitioning from starch to oil.
authors: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA.
authors: Sanjaya
authors: Timothy P
authors: TP
authors: Durrett
authors: Sean E
authors: SE
authors: Weise
authors: Christoph
authors: C
authors: Benning
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000235:genetics / Q000378:metabolism; D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001937:Brassica / Q000235:genetics; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D018274:Electroporation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005818:Genetic Engineering / Q000379:methods; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000235:genetics / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D010938:Plant Oils / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference; D012639:Seeds / Q000378:metabolism; D013213:Starch / Q000096:biosynthesis*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D000213:Acyl Carrier Protein; D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D007527:Isoenzymes; D010938:Plant Oils; C036483:T-DNA; D014157:Transcription Factors; D014280:Triglycerides; C495665:WRINKLED1 protein, Arabidopsis; D002244:Carbon; D013213:Starch; D005964:Glucosyltransferases; C022699:sucrose synthase; D051599:Glucose-1-Phosphate Adenylyltransferase; D000103:Acetyl-CoA Carboxylase; C557634:BCCP2 protein, Arabidopsis