title: Formation of post-confluence structure in human parotid gland acinar cells on PLGA through regulation of E-cadherin.
abstract: As a potential solution for patients to retrieve their lost salivary gland functions, tissue engineering of an auto-secretory device is profoundly needed. Under serum-free environment, primary human parotid gland acinar (PGAC) cells can be obtained. After reaching confluence, PGAC cells spontaneously form three-dimension (3D) cell aggregations, termed post-confluence structure (PCS), and change their behaviors. Poly (lactic-co-glycolic acid) (PLGA) has been widely used in the field of biomedical applications because of its biodegradable properties for desired functions. Nonetheless, the role of PLGA in facilitating PGAC cells to form PCS has seldom been explored to recover epithelial characteristics. In this study, PGAC cells were found to have a greater tendency to form PCS on PLGA than on tissue culture polystyrene (TCPS). By tracing cell migration paths and modulating E-cadherin activity with specific inhibitor or antibody, we demonstrated that the static force of homophilic interaction on surfaces of individual cells, but not the dynamics of cell migration, played a more important role in PCS formation. Thus, PLGA was successfully confirmed to support PGAC cells to form more PCS through the effects on enhancing E-cadherin expression, which is associated with FAK/ILK/Snail expression in PGAC cells. This result indicates that selective appropriate biomaterials may be potentially useful in generating 3D PCS on two-dimension (2D) substrate without fabricating a complex 3D scaffold.
authors: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
authors: Yen-Hui
authors: YH
authors: Chan
authors: Tsung-Wei
authors: TW
authors: Huang
authors: Ya-Shuan
authors: YS
authors: Chou
authors: Sheng-Hao
authors: SH
authors: Hsu
authors: Wei-Fang
authors: WF
authors: Su
authors: Pei-Jen
authors: PJ
authors: Lou
authors: Tai-Horng
authors: TH
authors: Young
mesh_terms: D061354:Acinar Cells / Q000166:cytology* / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010306:Parotid Gland / Q000166:cytology*; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D015854:Up-Regulation
chemical_list: D001672:Biocompatible Materials; D015820:Cadherins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid