title: Impact of taxol on dermal papilla cells--a proteomics and bioinformatics analysis.
abstract: Dermal papilla (DP) cells play a regulatory role in hair growth, and also play a role in alopecia (hair loss). However, effects of taxol, which is a widely used chemotherapy drug, on DP cells remain unclear, despite that theoretically taxol can impact on DP cells to contribute to taxol-induced alopecia. To better understand pathophysiology of taxol-induced damage in DP cells, morphological and biochemical analyses were performed to check whether taxol can cause apoptosis in cultured DP cells or not. If it can, proteomics and bioinformatics analyses were then performed to investigate the protein networks which are impacted by the taxol treatment. Our data showed that taxol can cause apoptotic damage in DP cells in a concentration-dependant manner, as demonstrated by various apoptotic markers. Proteomic analysis on DP cells treated with the lowest apoptosis-inducible concentration of taxol revealed that taxol can affect expression of proteins involved in Ca2+-regulated biological processes, vesicles transport, protein folding, reductive detoxification, and biomolecules metabolism. Furthermore, bioinformatics analysis indicated that taxol can impact on multiple biological networks. Taken together, this biochemical, proteomics, and bioinformatics data may give an insight into pathophysiology of taxol-induced damage in DP cells and shed light on mechanisms underlying taxol-induced alopecia.
authors: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
authors: Pei-Hsiu
authors: PH
authors: Chen
authors: Chih-Yuan
authors: CY
authors: Wang
authors: Ching-Wu
authors: CW
authors: Hsia
authors: Ming-Yi
authors: MY
authors: Ho
authors: Ann
authors: A
authors: Chen
authors: Min-Jen
authors: MJ
authors: Tseng
authors: Yung-Fu
authors: YF
authors: Wu
authors: Han-Min
authors: HM
authors: Chen
authors: Tzu-Hao
authors: TH
authors: Huang
authors: Hung-Te
authors: HT
authors: Liu
authors: Hao-Ai
authors: HA
authors: Shui
mesh_terms: D000505:Alopecia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D020405:Dermis / Q000378:metabolism* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D008297:Male; D009924:Organ Culture Techniques; D017239:Paclitaxel / Q000009:adverse effects* / Q000494:pharmacology; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D014738:Vibrissae / Q000378:metabolism* / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel; D002118:Calcium