title: Decreased expression of EBI3 and Foxp3 in CD4+CD25+ regulatory T cells in murine experimental allergic rhinitis.
abstract: BACKGROUND
  Epstein-Barr virus-induced gene 3 (EBI3) is a widely expressed interleukin-12-related protein that plays a critical role in the regulation of effector T cells and inflammatory responses.
  
  
  METHODS
  Allergic rhinitis (AR) was induced in mice by both intraperitoneal injection and intranasal administration of ovalbumin. By using immunohistochemical techniques, we investigated EBI3 expression in mouse spleens. The expressions of EBI3 and forkhead transcription factor 3 (Foxp3) in CD4+CD25+ regulatory T (T(reg)) cells isolated from spleens were assessed by flow cytometry. RNA extraction and RT-PCR were used to measure EBI3 and Foxp3 mRNA expression.
  
  
  RESULTS
  EBI3 and Foxp3 were expressed mainly in the white pulp of the spleens. Flow cytometry analysis showed that EBI3 and Foxp3 coexpressed in mouse CD4+CD25+ T(reg) cells. Furthermore, RT-PCR and flow cytometry analysis showed that expression of both Foxp3 and EBI3 was reduced in CD4+CD25+ T(reg) cells in an AR mouse model compared with controls, but there was no difference in the frequency of CD4+CD25+ T(reg) cells.
  
  
  CONCLUSIONS
  The deficiency in CD4+CD25+ T(reg) cell suppressive activity in AR is associated with the molecular mediators EBI3 and Foxp3.
authors: Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education), Beijing Institute of Otolaryngology, Beijing, China.
authors: Yuhui
authors: Y
authors: Ouyang
authors: Atsuhito
authors: A
authors: Nakao
authors: Erzhong
authors: E
authors: Fan
authors: Ying
authors: Y
authors: Li
authors: Liping
authors: L
authors: Zhao
authors: Wei
authors: W
authors: Zhang
authors: Demin
authors: D
authors: Han
authors: Luo
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D015704:CD4 Antigens / Q000378:metabolism; D002908:Chronic Disease; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D051379:Mice; D015778:Minor Histocompatibility Antigens; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; C499601:Ebi3 protein, mouse; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D015778:Minor Histocompatibility Antigens; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D010047:Ovalbumin