title: Halopenitus persicus gen. nov., sp. nov., an archaeon from an inland salt lake.
abstract: A novel pale pink-pigmented halophilic archaeon, strain DC30(T), was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain DC30(T) were non-motile and pleomorphic, from rods to triangular or disc-shaped. Strain DC30(T) required at least 1.7 M NaCl and 0.05 M MgCl(2) for growth (optimum, 3 M NaCl and 0.1 M MgCl(2)). The optimum pH and temperature for growth of strain DC30(T) were pH 7.5 and 40 °C, respectively, although it was capable of growth over pH and temperature ranges of 6.5-8.5 and 25-50 °C, respectively. Analysis of the 16S rRNA gene sequence showed that strain DC30(T) was a member of the family Halobacteriaceae. However, it had low 16S rRNA gene sequence similarities of 92.4%, 89.4% and 89.1% to the most closely related haloarchaeal taxa, the type species of the genera Halorubrum, Halogranum and Haloplanus, respectively. The DNA G+C content was 66.0 mol%. Phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, were present. Three minor phospholipids and one unidentified glycolipid were also observed. The only quinone present was MK-8(II-H(2)). The physiological, biochemical and phylogenetic differences between strain DC30(T) and other previously described genera of extremely halophilic archaea suggest that strain DC30(T) represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halopenitus persicus gen. nov., sp. nov. is proposed. The type strain of Halopenitus persicus is DC30(T) ( = IBRC 10041(T) = KCTC 4046(T)).
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.|Extremophile Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors: Mohammad Ali
authors: MA
authors: Amoozegar
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.|Extremophile Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors: Ali
authors: A
authors: Makhdoumi-Kakhki
authors: Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.|Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
authors: Seyed Abolhassan
authors: SA
authors: Shahzadeh Fazeli
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
authors: Reza
authors: R
authors: Azarbaijani
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.
authors: Antonio
authors: A
authors: Ventosa
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D060106:Lakes / Q000382:microbiology*; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D015636:Magnesium Chloride; D012965:Sodium Chloride