title: Comparative study of peripheral neuropathy and nerve regeneration in NOD and ICR diabetic mice.
abstract: The non-obese diabetic (NOD) mouse was suggested as an adequate model for diabetic autonomic neuropathy. We evaluated sensory-motor neuropathy and nerve regeneration following sciatic nerve crush in NOD males rendered diabetic by multiple low doses of streptozotocin, in comparison with similarly treated Institute for Cancer Research (ICR) mice, a widely used model for type I diabetes. Neurophysiological values for both strains showed a decline in motor and sensory nerve conduction velocity at 7 and 8 weeks after induction of diabetes in the intact hindlimb. However, amplitudes of compound muscle and sensory action potentials (CMAPs and CNAPs) were significantly reduced in NOD but not in ICR diabetic mice. Morphometrical analysis showed myelinated fiber loss in highly hyperglycemic NOD mice, but no significant changes in fiber size. There was a reduction of intraepidermal nerve fibers, more pronounced in NOD than in ICR diabetic mice. Interestingly, aldose reductase and poly(ADP-ribose) polymerase (PARP) activities were increased already at 1 week of hyperglycemia, persisting until the end of the experiment in both strains. Muscle and nerve reinnervation was delayed in diabetic mice following sciatic nerve crush, being more marked in NOD mice. Thus, diabetes of mid-duration induces more severe peripheral neuropathy and slower nerve regeneration in NOD than in ICR mice.
authors: Department of Biochemistry and Molecular Biology and Centre de Biotecnologia i Teràpia Gènica, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors: Judit
authors: J
authors: Homs
authors: Lorena
authors: L
authors: Ariza
authors: Gemma
authors: G
authors: Pagès
authors: Enrique
authors: E
authors: Verdú
authors: Laura
authors: L
authors: Casals
authors: Esther
authors: E
authors: Udina
authors: Miguel
authors: M
authors: Chillón
authors: Assumpció
authors: A
authors: Bosch
authors: Xavier
authors: X
authors: Navarro
mesh_terms: D000449:Aldehyde Reductase / Q000097:blood; D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000473:pathology*; D004576:Electromyography; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D009409:Nerve Crush; D009416:Nerve Regeneration
chemical_list: D000449:Aldehyde Reductase