title: Analysis of acid-soluble glycogen in pork extracts of two PRKAG3 genotypes by 1H liquid-state NMR spectroscopy and biochemical methods.
abstract: Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN(-) and rn(+) genotype) were analyzed by both (1)H liquid-state NMR spectroscopy and a biochemical method. The (1)H NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of α-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different post-mortem glycogen degradation mechanisms in the two genotypes.
authors: Department of Food Science, University of Copenhagen , Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. fhl@life.ku.dk
authors: Flemming H
authors: FH
authors: Larsen
authors: Birgitta
authors: B
authors: Essén-Gustavsson
authors: Marianne
authors: M
authors: Jensen-Waern
authors: René
authors: R
authors: Lametsch
authors: Anders H
authors: AH
authors: Karlsson
authors: Gunilla
authors: G
authors: Lindahl
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D006003:Glycogen / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D009154:Mutation; D013552:Swine / Q000235:genetics / Q000378:metabolism*
chemical_list: D006003:Glycogen; D055372:AMP-Activated Protein Kinases