title: Molecular characterization of a canine coronavirus NA/09 strain detected in a dog's organs.
abstract: In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.
authors: Department of Anatomy and Physiology of Farm Animals, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece. vasilisntafis@hotmail.com
authors: Vasileios
authors: V
authors: Ntafis
authors: Eftychia
authors: E
authors: Xylouri
authors: Viviana
authors: V
authors: Mari
authors: Maria
authors: M
authors: Papanastassopoulou
authors: Nikolaos
authors: N
authors: Papaioannou
authors: Angelos
authors: A
authors: Thomas
authors: Canio
authors: C
authors: Buonavoglia
authors: Nicola
authors: N
authors: Decaro
mesh_terms: D000818:Animals; D001483:Base Sequence; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D007422:Intestines / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny