title: Inverse electron-demand Diels-Alder reactions for the selective and efficient labeling of RNA.
abstract: We report the first example of RNA labeling based on inverse electron-demand Diels-Alder reactions. Both chemically synthesized and enzymatically transcribed RNAs were successfully modified with biotin or a fluorescent label. This approach works efficiently under mild conditions in water and does not require transition metals.
authors: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg 69120, Germany.
authors: Juliane
authors: J
authors: Schoch
authors: Sandeep
authors: S
authors: Ameta
authors: Andres
authors: A
authors: Jäschke
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004583:Electrons; D009943:Organophosphorus Compounds / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012313:RNA; D012321:DNA-Directed RNA Polymerases