title: Efficient production of L-lactic acid from xylose by a recombinant Candida utilis strain.
abstract: Efficient L-lactic acid production from xylose was achieved using a pyruvate decarboxylase-deficient Candida utilis strain expressing an L-lactate dehydrogenase, an NADH-preferring mutated xylose reductase (XR), a xylitol dehydrogenase and a xylulokinase. The recombinant strain showed 53% increased L-lactic acid production compared with the reference strain expressing native XR (NADPH-preferring).
authors: Central Laboratories for Frontier Technology, KIRIN Holdings Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan. Hideyuki_Tamakawa@kirin.co.jp
authors: Hideyuki
authors: H
authors: Tamakawa
authors: Shigehito
authors: S
authors: Ikushima
authors: Satoshi
authors: S
authors: Yoshida
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D050542:D-Xylulose Reductase / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005822:Genetic Vectors; D007218:Industrial Microbiology; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D016297:Mutagenesis, Site-Directed; D030781:Organisms, Genetically Modified / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D013696:Temperature; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D014994:Xylose; D000449:Aldehyde Reductase; D007770:L-Lactate Dehydrogenase; D050542:D-Xylulose Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase