title: [Autophagy inhibitor 3-MA decreases the production and release of infectious enterovirus 71 particles].
abstract: OBJECTIVE
  To determine whether or not enterovirus 71 (enteroviurs 71, EV71) may induce autophagy and affect the production and release of EV71 after the treatment of autophagy inhibitor.
  
  
  METHODS
  Western blots were performed to examine the conversion of LC3-I to LC3- II and the degradation of P62 after the RD-A cells were infected with EV71. CCID50 was determined by checking the virus titer in the supernatant of cells that treated with autophagy inhibitor 3-MA.
  
  
  RESULTS
  EV71 infection enhances the type conversion of LC3 and degradation of P62. The infectious virus particles were decreased after the treatment of 3-MA.
  
  
  CONCLUSIONS
  EV71 infection could induce cell autophagy and the autophagy might contribute to the production and release of infectious EV71 particles.
authors: State Key Lab for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100005, China.
authors: Xiao-Yan
authors: XY
authors: Zhang
authors: Xue-Yan
authors: XY
authors: Xi
authors: Zhen-Dong
authors: ZD
authors: Zhao
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001343:Autophagy / Q000187:drug effects*; D045744:Cell Line, Tumor; D004770:Enterovirus / Q000187:drug effects*; D006801:Humans
chemical_list: D000998:Antiviral Agents; C025946:3-methyladenine; D000225:Adenine