title: Impulsive pressurization of neuronal cells for traumatic brain injury study.
abstract: A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration.
authors: Department of Engineering Mechanics, University of Nebraska-Lincoln, USA.
authors: Matthew
authors: M
authors: Nienaber
authors: Jeong Soon
authors: JS
authors: Lee
authors: Ruqiang
authors: R
authors: Feng
authors: Jung Yul
authors: JY
authors: Lim
mesh_terms: D001696:Biomechanical Phenomena; D001753:Blast Injuries / Q000473:pathology*; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology*; D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000473:pathology*; D011312:Pressure