title: The wheat gene TaST can increase the salt tolerance of transgenic Arabidopsis.
abstract: On the basis of the results of gene chip analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR analysis showed that the expression of the gene was induced by salt stress. Transgenic Arabidopsis plants overexpressing the TaST gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein encoded by this gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca(2+), soluble sugar, and proline and less Na(+) under salt stress. Real-time quantitative PCR analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants.
authors: College of Life Science of Hebei Normal University, No. 113 Yuhua East Road, Shijiazhuang, Hebei, People's Republic of China.
authors: Xi
authors: X
authors: Huang
authors: Gang
authors: G
authors: Wang
authors: Yinzhu
authors: Y
authors: Shen
authors: Zhanjing
authors: Z
authors: Huang
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D018525:Germination / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D002734:Chlorophyll; D049452:Green Fluorescent Proteins