title: Oxysterol-binding protein (OSBP) enhances replication of intracellular Salmonella and binds the Salmonella SPI-2 effector SseL via its N-terminus.
abstract: Effectors translocated into the host cell by Salmonella enterica serovar Typhimurium are critical for bacterial virulence. For many effectors, the mechanisms of their interactions with host pathways are not yet understood. We have recently found an interaction between the SPI-2 effector SseL and oxysterol-binding protein (OSBP). We show here that SseL binds the N-terminus of OSBP and that S. Typhimurium infection results in redistribution of OSBP. We furthermore demonstrate that OSBP is required for efficient replication of intracellular S. Typhimurium. This suggests that S. Typhimurium hijacks OSBP-dependent pathways to benefit its intracellular life-style, possibly by SseL- and OSBP-mediated manipulation of host lipid metabolism.
authors: Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada.
authors: Sigrid D
authors: SD
authors: Auweter
authors: Hong B
authors: HB
authors: Yu
authors: Ellen T
authors: ET
authors: Arena
authors: Julian A
authors: JA
authors: Guttman
authors: B Brett
authors: BB
authors: Finlay
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D042541:Intracellular Space / Q000382:microbiology; D008565:Membrane Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011987:Receptors, Steroid / Q000378:metabolism*; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000472:pathogenicity / Q000502:physiology*; D000098825:Oxysterol Binding Proteins
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D011987:Receptors, Steroid; C440638:SPI-2 protein, Salmonella; D000098825:Oxysterol Binding Proteins; D010450:Endopeptidases