title: Follicular proinflammatory cytokines and chemokines as markers of IVF success.
abstract: Cytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammatory: G-CSF), chemokines (MIP-1α, MIP-1β, MCP-1, RANTES, and IL-8), and other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoing in vitro fertilization (IVF). Cytokine origin was studied by mRNA analysis of granulosa cells. Higher follicular MIP-1α and CD44(v6) were found to correlate with polycystic ovary syndrome, IL-23, INF-γ, and TNF-α with endometriosis, higher CD44(v6) but lower IL-β and INF-α correlated with tubal factor infertility, and lower levels of IL-18 and CD44(v6) characterized unexplained infertility. IL-12 positively correlated with oocyte fertilization and embryo development, while increased IL-18, IL-8, and MIP-1β were associated with successful IVF-induced pregnancy.
authors: Department of Immunology, Institute of General and Molecular Pathology, University of Tartu, Ravila 19, Tartu 50411, Estonia.
authors: Aili
authors: A
authors: Sarapik
authors: Agne
authors: A
authors: Velthut
authors: Kadri
authors: K
authors: Haller-Kikkatalo
authors: Gilbert C
authors: GC
authors: Faure
authors: Marie-Christine
authors: MC
authors: Béné
authors: Marcelo
authors: M
authors: de Carvalho Bittencourt
authors: Frédéric
authors: F
authors: Massin
authors: Raivo
authors: R
authors: Uibo
authors: Andres
authors: A
authors: Salumets
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D057166:High-Throughput Screening Assays; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis* / Q000473:pathology / Q000503:physiopathology / Q000628:therapy; D018836:Inflammation Mediators / Q000378:metabolism*; D006080:Ovarian Follicle / Q000473:pathology; D011247:Pregnancy; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators