title: LC-MS/MS quantification of bioactive angiotensin I-converting enzyme inhibitory peptides in rye malt sourdoughs.
abstract: This study quantified antiotensin I-converting enzyme (ACE) inhibitory peptides in rye malt sourdoughs supplemented with gluten proteins and fermented with six strains of Lactobacillus spp. Bioinformatic analysis of prolamins from barley, rye, and wheat demonstrated that the ACE inhibitory peptides LQP, LLP, VPP, and IPP are frequently encrypted in their primary sequence. These tripeptides were quantified by liquid chromatography-tandem mass spectrometry. Tripeptide levels in sourdoughs were generally higher as compared to the chemically acidified controls. Sourdoughs fermented with different strains showed different concentrations of LQP and LLP. These differences corresponded to strain-specific differences in PepO and PepN activities. The highest levels of peptides VPP, IPP, LQP, and LLP, 0.23, 0.71, 1.09, and 0.09 mmol (kg DM)(-1), respectively, were observed in rye malt: gluten sourdoughs fermented with Lactobacillus reuteri TMW 1.106 and added protease. These concentrations were 6-7 times higher as compared to sourdough without fungal protease and exceed the IC(50) by 100-1000-fold.
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, 410 Ag/For Centre, Edmonton, Alberta T6G2P5, Canada.
authors: Ying
authors: Y
authors: Hu
authors: Achim
authors: A
authors: Stromeck
authors: Jussi
authors: J
authors: Loponen
authors: Daise
authors: D
authors: Lopes-Lutz
authors: Andreas
authors: A
authors: Schieber
authors: Michael G
authors: MG
authors: Gänzle
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D001939:Bread / Q000032:analysis* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005433:Flour / Q000032:analysis / Q000382:microbiology; D001467:Hordeum / Q000737:chemistry / Q000382:microbiology; D007778:Lactobacillus / Q000378:metabolism; D010455:Peptides / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D055315:Prolamins / Q000032:analysis / Q000378:metabolism; D012434:Secale / Q000737:chemistry* / Q000382:microbiology; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000737:chemistry / Q000382:microbiology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D055315:Prolamins