title: Production of CCHF virus-like particle by a baculovirus-insect cell expression system.
abstract: Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
authors: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China.
authors: Zhao-Rui
authors: ZR
authors: Zhou
authors: Man-Li
authors: ML
authors: Wang
authors: Fei
authors: F
authors: Deng
authors: Tian-Xian
authors: TX
authors: Li
authors: Zhi-Hong
authors: ZH
authors: Hu
authors: Hua-Lin
authors: HL
authors: Wang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D015153:Blotting, Western; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D022701:Virosomes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D022701:Virosomes