title: TFPI-2 methylation predicts poor prognosis in non-small cell lung cancer.
abstract: BACKGROUND
  Methylation of human tissue factor pathway inhibitor-2 (TFPI-2) gene has been detected in several types of cancer, including non-small cell lung cancer (NSCLC). However, an association between the methylation status of TFPI-2 gene and prognosis has not yet been investigated.
  
  
  METHODS
  Methylation of TFPI-2 gene was examined in a consecutive series of 133 non-metastatic NSCLC patients using methylation-specific PCR (MSP). Univariate and multivariate analyses were conducted to investigate the association between clinical variables and overall survival time.
  
  
  RESULTS
  Methylation of TFPI-2 gene was detected in 36 of 133 patients (27.1%). Of these 36 patients, seventeen individuals (47.2%) carried stage III tumors. The 5-year disease free survival rate among patients carrying methylated TFPI-2 tumors was significantly lower as compared to those with unmethylated TFPI-2 tumors (35.5% versus 6.1%, P<0.0001). Moreover, methylation of TFPI-2 gene was found to be an independent prognostic factor for poor overall survival based on multivariate analysis models (P=0.013), as was age >62 years old (P<0.0001) and TNM stage of disease (P<0.0001).
  
  
  CONCLUSIONS
  The results of the present study suggest that methylation of TFPI-2 gene is an independent factor for an unfavorable prognosis in patients with NSCLC.
authors: Department of Thoracic-Cardiac Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, 107 Yanjiangxi Rd., Guangzhou 510120, PR China.
authors: Duoguang
authors: D
authors: Wu
authors: Lihua
authors: L
authors: Xiong
authors: Shuoming
authors: S
authors: Wu
authors: Ming
authors: M
authors: Jiang
authors: Guiyong
authors: G
authors: Lian
authors: Minghui
authors: M
authors: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018287:Carcinoma, Large Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm; D006023:Glycoproteins; D012334:RNA, Neoplasm; C021240:tissue-factor-pathway inhibitor 2