title: Influence of minor backbone structural variations in modulating the in vitro gene transfer efficacies of α-tocopherol based cationic transfection lipids.
abstract: Toward probing the influence of backbone structural variation in cationic lipid mediated gene delivery of α-tocopherol based lipids, two novel α-tocopherol based lipids 1 and 2 have been designed and synthesized. The only structural difference between the cationic amphiphiles 1 and 2 is the backbone structure, where lipid 1 has a non-glycerol backbone and lipid 2 has a glycerol backbone. The lipids 1 and 2 showed contrasting transfection efficiencies: lipid 1 showed high gene transfer efficacy in multiple cultured animals cell lines, whereas lipid 2 is transfection incompetent. In summary, the present findings demonstrate that in the case of α-tocopherol based lipids even minor structural variations like backbone can profoundly influence size, DNA binding characteristics, cellular uptake, and consequently gene delivery efficacies.
authors: Department of Chemistry, National Institute of Technology, Warangal, Andhra Pradesh, India.
authors: Bhavani
authors: B
authors: Kedika
authors: Srilakshmi V
authors: SV
authors: Patri
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D004247:DNA / Q000008:administration & dosage*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage*; D014162:Transfection; D024502:alpha-Tocopherol / Q000737:chemistry*
chemical_list: D002412:Cations; D008055:Lipids; D004247:DNA; D024502:alpha-Tocopherol