title: MiR-886-3p regulates cell proliferation and migration, and is dysregulated in familial non-medullary thyroid cancer.
abstract: BACKGROUND
  The molecular basis and characteristics of familial non-medullary thyroid cancer are poorly understood. In this study, we performed microRNA (miRNA) profiling of familial and sporadic papillary thyroid cancer tumor samples.
  
  
  RESULTS
  Genome wide miRNA profiling of sporadic and familial papillary thyroid cancer was performed. Differentially expressed miRNAs were validated by quantitative RT-PCR. Ectopic expression of miR-886-3p in thyroid cancer lines was performed to identify pathways targeted by the miRNA, as well as, to determine its effect on tumor cell biology. We found four differentially expressed miRNAs between familial and sporadic papillary thyroid cancer tumor samples. MiR-886-3p and miR-20a were validated to be differentially expressed by 3- and 4-fold, respectively. Pathway analysis of genome-wide expression data on cells overexpressing miR-886-3p and target prediction analysis showed genes involved in DNA replication and focal adhesion pathways were regulated by miR-886-3p. Overexpression of miR-886-3p in thyroid cancer cell lines significantly inhibited cellular proliferation, the number and size of spheroids and cellular migration. Additionally, overexpression of miR-886-3p increased the number of cells in S phase.
  
  
  CONCLUSIONS
  Our findings for the first time suggest that miR-886-3p plays an important role in thyroid cancer tumor cell biology and regulates genes involved in DNA replication and focal adhesion. Thus, miR-886-3p may play a role in the initiation and or progression of papillary thyroid cancer.
authors: Endocrine Oncology Section, Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America.
authors: Yin
authors: Y
authors: Xiong
authors: Lisa
authors: L
authors: Zhang
authors: Alisha K
authors: AK
authors: Holloway
authors: Xiaolin
authors: X
authors: Wu
authors: Ling
authors: L
authors: Su
authors: Electron
authors: E
authors: Kebebew
mesh_terms: D001483:Base Sequence; D002277:Carcinoma; D018276:Carcinoma, Medullary / Q000151:congenital; D002291:Carcinoma, Papillary; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D004261:DNA Replication / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D022001:Focal Adhesions / Q000235:genetics; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D018813:Multiple Endocrine Neoplasia Type 2a; D009386:Neoplastic Syndromes, Hereditary; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology
chemical_list: C559060:MIRN886 microRNA, human; D035683:MicroRNAs; D012322:RNA Precursors