title: A reference proteomic database of Lactobacillus plantarum CMCC-P0002.
abstract: Lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. In this study, the whole-cell proteins and secretory proteins of L. plantarum were separated by two-dimensional electrophoresis method. A total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein pLP9000_05. The information of first 20 highest abundance proteins was listed for the further genetic manipulation of L. plantarum, such as construction of high-level expressions system. Furthermore, the first interaction map of L. plantarum was established by Blue-Native/SDS-PAGE technique. A heterodimeric complex composed of maltose phosphorylase Map3 and Map2, and two homodimeric complexes composed of Map3 and Map2 respectively, were identified at the same time, indicating the important roles of these proteins. These findings provided valuable information for the further proteomic researches of L. plantarum.
authors: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.
authors: Li
authors: L
authors: Zhu
authors: Wei
authors: W
authors: Hu
authors: Datao
authors: D
authors: Liu
authors: Wanhong
authors: W
authors: Tian
authors: Gang
authors: G
authors: Yu
authors: Xiankai
authors: X
authors: Liu
authors: Jie
authors: J
authors: Wang
authors: Erling
authors: E
authors: Feng
authors: Xuemin
authors: X
authors: Zhang
authors: Bei
authors: B
authors: Chen
authors: Ming
authors: M
authors: Zeng
authors: Hengliang
authors: H
authors: Wang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D030562:Databases, Protein / Q000592:standards*; D015180:Electrophoresis, Gel, Two-Dimensional; D048191:Lactiplantibacillus plantarum; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D012015:Reference Standards; D012995:Solubility; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome