title: [The effects of a histone deacetylase (HDAC) inhibitor on endotoxin-induced endothelial cell injury].
abstract: OBJECTIVE
  To examine the effects of HDAC inhibitor trichostatin A (TSA) on lipopolysaccharide (LPS) induced genes expression in cultured endothelial cells in order to understand the mechanisms involved in the protection of endothelial cells against vascular endothelium injury during endotoxemia.
  
  
  METHODS
  Cultured EAhy926 cells (a cell line with the features of vascular endothelial cells) were treated with LPS at 100 ng/ml [the dose was chosen for minimum impact on cell survival as determined using Methyl thiazolyl tetrazolium (MTT) assay], or LPS and TSA (100 μg/ml). The expressions of toll-like receptor 4 (TLR4) and HDAC2 were measured by Western blotting 3, 6, 9, 12 and 24 hours after the beginning of the treatment.
  
  
  RESULTS
  The expressions of TLR4 (as measured by densitometry) was found significantly higher (P< 0.01) in LPS treated cells (1.01±0.14, 1.25±0.16, 1.20±0.19) 9, 12 and 24 hours after the beginning of the treatment as compared to untreated cells (0.34±0.05); The expression of HDAC2 was also found significantly higher (P< 0.01) after LPS treatment for 12 and 24 hours (1.14±0.10, 1.20±0.04) in comparison with untreated control (0.17±0.02). In LPS+TSA treated cells, TLR4 expression (0.37±0.07) was significantly lower (P< 0.05) after 12 hours of treatment in comparison with its LPS treated counterpart (1.25±0.16), while the level of TLR4 expression stayed unchanged 24 hours after the beginning of the treatment, as compared to the result at 12 hours (0.37±0.10 vs. 1.20±0.19, P> 0.05). No visible HDAC2 expression was detected in the cells treated with LPS+TSA.
  
  
  CONCLUSIONS
  LPS stimulated the increase of TLR4 and HDAC2 expression in the vascular endothelial cells studied while TSA suppress the LPS induced TLR4 and HDAC2 expression in these cells.
authors: Department of Critical Care Medicine, Capital Medical University, Beijing, China.
authors: Hui-min
authors: HM
authors: He
authors: Ang
authors: A
authors: Li
authors: Shu-wen
authors: SW
authors: Zhang
authors: Mei-li
authors: ML
authors: Duan
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D056464:Histone Deacetylase 2 / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D008070:Lipopolysaccharides; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C012589:trichostatin A; C533423:HDAC2 protein, human; D056464:Histone Deacetylase 2