title: [Construction and recovery of chimeric rabies virus expressing envelop proteins E1E2 of hepatitis C].
abstract: OBJECTIVE
  Construction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2.
  
  
  METHODS
  On the basis of the previously established reverse genetic system CTN-GFP, HCV E1E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNdeltaG-HCV E1E2 were recovered.
  
  
  RESULTS
  The result demonstrated that both the chimeric viruses were rescued successfully, had the ability to re-infect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA.
  
  
  CONCLUSIONS
  The establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.
authors: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Jia-Liang
authors: JL
authors: Du
authors: Ying
authors: Y
authors: Huang
authors: Qing
authors: Q
authors: Tang
authors: Li-Hua
authors: LH
authors: Wang
authors: Guo-Dong
authors: GD
authors: Liang
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism*; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins