title: Identification and expression analysis of the Broad-Complex  core protein isoform 6 (BR-C Z6) gene in the giant tiger  shrimp Penaeus monodon (Penaeidae: Decapoda).
abstract: Broad-Complex (BR-C) is an early ecdysone-responsive gene  encoding a family of zinc-finger transcription factors that function during  metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in  the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in  length, containing open reading frames of 1440 and 1443 bp, corresponding to  polypeptides of 479 and 480 amino acids, respectively. Tissue distribution  analysis indicated that PmBR-C Z6 was abundantly expressed in hemocytes  and ovaries in juveniles. In broodstock, PmBR-C Z6 was constitutively  expressed in all tissues examined, and the highest expression was observed in  ovaries. The expression of PmBR-C Z6 in ovaries was significantly greater  than in testes in both juveniles and broodstock of P. monodon.  Quantitative real-time PCR indicated that the expression level of PmBR-C Z6 was  significantly down-regulated in stages II and III of ovaries in intact wild  broodstock and returned to the basal level in stage IV ovaries and after  spawning. In eyestalk-ablated broodstock, PmBR-C Z6 was significantly  up-regulated in stage IV (mature) ovaries. Moreover, the expression level of PmBR-C  Z6 in vitellogenic, early cortical rod and mature (stages II-IV) ovaries of  eyestalk-ablated broodstock was greater than that of the same ovarian stages in  intact broodstock. In situ hybridization revealed that PmBR-C Z6 transcripts  were localized in oogonia and cytoplasm of previtellogenic and vitellogenic  oocytes of both wild intact and eyestalk-abated broodstock. The effects of  20-hydroxyecdysone on expression of PmBR-C Z6 were examined. The  expression level of PmBR-C Z6 in ovaries of juvenile P. monodon was  significantly increased at 168 h post-injection. Taken together, these findings  indicate that PmBR-C Z6 plays an important role in ovarian development  of P. monodon.
authors: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors: A
authors: A
authors: Buaklin
authors: S
authors: S
authors: Klinbunga
authors: P
authors: P
authors: Mensveta
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000096:biosynthesis / Q000235:genetics; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008297:Male; D009928:Organ Specificity / Q000187:drug effects / Q000502:physiology; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism
chemical_list: D060829:Arthropod Proteins; D004441:Ecdysterone