title: Co-interactive DNA-binding between a novel, immunophilin-like shrimp protein and VP15 nucleocapsid protein of white spot syndrome virus.
abstract: White spot syndrome virus (WSSV) is one of the most serious pathogens of penaeid shrimp. Although its genome has been completely characterized, the functions of most of its putative proteins are not yet known. It has been suggested that the major nucleocapsid protein VP15 is involved in packaging of the WSSV genome during virion formation. However, little is known in its relationship with shrimp host cells. Using the yeast two-hybrid approach to screen a shrimp lymphoid organ (LO) cDNA library for proteins that might interact with VP15, a protein named PmFKBP46 was identified. It had high sequence similarity to a 46 kDa-immunophilin called FKBP46 from the lepidopteran Spodoptera frugiperda (the fall armyworm). The full length PmFKBP46 consisted of a 1,257-nucleotide open reading frame with a deduced amino acid sequence of 418 residues containing a putative FKBP-PPIase domain in the C-terminal region. Results from a GST pull-down assay and histological co-localization revealed that VP15 physically interacted with PmFKBP46 and that both proteins shared the same subcellular location in the nucleus. An electrophoretic mobility shift assay indicated that PmFKBP46 possessed DNA-binding activity and functionally co-interacted with VP15 in DNA binding. The overall results suggested that host PmFKBP46 might be involved in genome packaging by viral VP15 during virion assembly.
authors: Centex Shrimp, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors: Pakkakul
authors: P
authors: Sangsuriya
authors: Saengchan
authors: S
authors: Senapin
authors: Wei-Pang
authors: WP
authors: Huang
authors: Chu-Fang
authors: CF
authors: Lo
authors: Timothy W
authors: TW
authors: Flegel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006434:Hemocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D033561:Penaeidae / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D021381:Protein Transport; D017386:Sequence Homology, Amino Acid; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D019065:Virus Assembly; D046848:White spot syndrome virus 1 / Q000378:metabolism* / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D019590:Nucleocapsid Proteins; C446942:VP15 protein, white spot syndrome virus; D004247:DNA; C403471:FKBP46; D022021:Tacrolimus Binding Proteins