title: Plasma rich in growth factors (PRGF-Endoret) stimulates tendon and synovial fibroblasts migration and improves the biological properties of hyaluronic acid.
abstract: OBJECTIVE
  Cell migration plays an essential role in development, wound healing, and tissue regeneration. Plasma rich in growth factors (PRGF-Endoret) technology offers a potential source of growth factors involved in tissue regeneration. Here, we evaluate the potential of PRGF-Endoret over tendon cells and synovial fibroblasts migration and study whether the combination of this autologous technology with hyaluronic acid (HA) improves the effect and potential of the biomaterials over the motility of both types of fibroblasts.
  
  
  METHODS
  Migration of primary tendon cells and synovial fibroblasts after culturing with either PRGF or PPGF (plasma poor in growth factors) at different doses was evaluated. Furthermore, the migratory capacity induced by the combination of PPGF and PRGF with HA was tested.
  
  
  RESULTS
  PPGF stimulated migration of both types of cells but this effect was significantly higher when PRGF was used. Tendon cells showed an increase of 212% in migratory ability when HA was combined with PPGF and of 335% in the case of HA + PRGF treatment compared with HA alone.
  
  
  CONCLUSIONS
  PRGF-Endoret stimulates migration of tendon cells and synovial fibroblasts and improves the biological properties of HA.
authors: Private Practice in Implantology and Oral Rehabilitation in Vitoria, Vitoria, Spain. eduardoanitua@eduardoanitua.com
authors: E
authors: E
authors: Anitua
authors: M
authors: M
authors: Sanchez
authors: M
authors: M
authors: De la Fuente
authors: M M
authors: MM
authors: Zalduendo
authors: G
authors: G
authors: Orive
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D010949:Plasma; D012038:Regeneration / Q000502:physiology*; D013583:Synovial Membrane / Q000166:cytology*; D013710:Tendons / Q000166:cytology* / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins