title: Dual crosslinked hydrogel nanoparticles by nanogel bottom-up method for sustained-release delivery.
abstract: Polysaccharide-PEG hybrid nanogels (CHPOA-PEGSH) crosslinked by both covalent ester bonds and physical interactions were prepared by the reaction of a thiol-modified poly(ethylene glycol) (PEGSH) with acryloyl-modified cholesterol-bearing pullulan (CHPOA). Experimental parameters, including CHPOA concentration, the degree of acryloyl substitution of CHPOA, and the initial amounts of CHPOA and PEGSH, were modified in order to assess their effect on the size of the nanogels (50-150 nm) and on their degradation kinetics, monitored by dynamic light scattering (DLS) and asymmetrical flow field-flow fractionation (AF4) chromatography. Rhodamine-labeled nanogels were injected intravenously into mice and their concentration in blood was determined by a fluorescence assay as a function of post-injection time. The elimination half-life (t(1/2)) of CHPOA-PEGSH nanoparticles was about 15-fold longer (18 h) than that of CHP nanogels (1.2 h). The half-life enhancement of CHPOA-PEGSH was attributed to the presence of the crosslinker PEG chains, which prevent non-specific protein adsorption, and to the slow hydrolysis kinetics of the crosslinking esters in the biological milieu. The hybrid CHPOA-PEGSH nanogels are expected to be useful as injectable nanocarriers for drugs and proteins, in view of their low surface fouling and slow hydrolysis rate.
authors: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Tokyo 101-0062, Japan.
authors: Asako
authors: A
authors: Shimoda
authors: Shin-ichi
authors: S
authors: Sawada
authors: Arihiro
authors: A
authors: Kano
authors: Atsushi
authors: A
authors: Maruyama
authors: Alexandre
authors: A
authors: Moquin
authors: Françoise M
authors: FM
authors: Winnik
authors: Kazunari
authors: K
authors: Akiyoshi
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry; D003432:Cross-Linking Reagents; D004337:Drug Carriers / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005456:Fluorescent Dyes; D005936:Glucans / Q000737:chemistry*; D006207:Half-Life; D020100:Hydrogels / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D006868:Hydrolysis; D007275:Injections, Intravenous; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012235:Rhodamines; D012542:Scattering, Radiation
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D005456:Fluorescent Dyes; D005936:Glucans; D020100:Hydrogels; D012235:Rhodamines; D011092:Polyethylene Glycols; C009109:pullulan; D002784:Cholesterol