title: Prospective on Mycobacterium tuberculosis proteomics.
abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, remains one of the most prevalent human pathogens in the world. Knowledge regarding the bacilli's physiology as well as its mechanisms of virulence, immunogenicity, and pathogenesis has increased greatly in the last three decades. However, the function of about one-quarter of the Mtb coding genome and the precise activity and protein networks of most of the Mtb proteins are still unknown. Protein mass spectrometry and a new interest in research toward the field of functional proteomics have given a new light to the study of this bacillus and will be the focus of this review. We will also discuss new perspectives in the proteomics field, in particular targeted mass spectrometry methods and their potential applications in TB research and discovery.
authors: Department of Microbiology, Immunology and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, Colorado 80523, United States.
authors: M Carolina
authors: MC
authors: Mehaffy
authors: Nicole A
authors: NA
authors: Kruh-Garcia
authors: Karen M
authors: KM
authors: Dobos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D058977:Molecular Sequence Annotation; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D010446:Peptide Fragments; D020543:Proteome