title: Analysis of the 3'UTR of the prostaglandin synthetase-2 (PTGS-2/COX-2) gene in non-melanoma skin cancer after organ transplantation.
abstract: To define the potential involvement of polymorphisms in the 3'untranslated region (3'UTR) of the prostaglandin synthetase-2 (PTGS-2) gene to non-melanoma skin cancer (NMSC) predisposition after transplantation, we screened for genetic variant, relevant parts of this region. It contains binding sites for trans-acting factors, an alternative polyadenylation site and putative target sequences for miRNAs. Variant +8473T>C did not appear to play a functional role in the regulation of gene expression in human keratinocyte-transfected cells. In addition to the well-known +8473T>C, we identified four polymorphisms: +8293G>C, +10259T>G, +10267G>A and +10335G>A. No allele frequency differences were observed between cases and controls neither for +8473T>C nor for any of the identified polymorphisms, suggesting that polymorphisms in the 3'UTR of the PTGS2 gene are rare and unlikely to represent risk factor for NMSC after transplantation.
authors: Macarena
authors: M
authors: Gomez-Lira
authors: Gianpaolo
authors: G
authors: Tessari
authors: Silvia
authors: S
authors: Mazzola
authors: Giovanni
authors: G
authors: Malerba
authors: Carlo
authors: C
authors: Rugiu
authors: Luigi
authors: L
authors: Naldi
authors: Francesco
authors: F
authors: Nacchia
authors: Francesca
authors: F
authors: Valerio
authors: Brunello
authors: B
authors: Anna
authors: Alberto
authors: A
authors: Forni
authors: Luigino
authors: L
authors: Boschiero
authors: Silvio
authors: S
authors: Sandrini
authors: Giuseppe
authors: G
authors: Faggian
authors: Giampiero
authors: G
authors: Girolomoni
authors: Alberto
authors: A
authors: Turco
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D001913:Bowen's Disease / Q000209:etiology / Q000235:genetics; D002280:Basal Cell Carcinoma / Q000209:etiology / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D007636:Keratoacanthoma / Q000209:etiology / Q000235:genetics; D016377:Organ Transplantation / Q000009:adverse effects*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012878:Skin Neoplasms / Q000209:etiology / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human