title: Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection.
abstract: The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express the gene under the control of a strong constitutive promoter, CaMV35S (Cauliflower Mosaic Virus). Based on analyses of tolerance to salinity stress and Pseudomonas syringae pv. tabaci (Pst) infection, the overexpression of GhCyp1 in transgenic plants conferred higher tolerance to salt stress and Pst infection compared with control plants. Therefore, we suggest that GhCyp1 may be a suitable candidate gene to produce transgenic plants with tolerance to abiotic and biotic stresses.
authors: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, PR China.
authors: Chuanfeng
authors: C
authors: Zhu
authors: Yixue
authors: Y
authors: Wang
authors: Yuanbao
authors: Y
authors: Li
authors: Khizar Hayat
authors: KH
authors: Bhatti
authors: Yingchuan
authors: Y
authors: Tian
authors: Jiahe
authors: J
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D021983:Cyclophilins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D018547:Plant Stems / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000502:physiology*; D018749:RNA, Plant / Q000235:genetics; D055049:Salt Tolerance; D036226:Seedlings / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D018749:RNA, Plant; D012965:Sodium Chloride; D021983:Cyclophilins