title: α-Ketoglutarate coordinates carbon and nitrogen utilization via enzyme I inhibition.
abstract: Microbes survive in a variety of nutrient environments by modulating their intracellular metabolism. Balanced growth requires coordinated uptake of carbon and nitrogen, the primary substrates for biomass production. Yet the mechanisms that balance carbon and nitrogen uptake are poorly understood. We find in Escherichia coli that a sudden increase in nitrogen availability results in an almost immediate increase in glucose uptake. The concentrations of glycolytic intermediates and known regulators, however, remain homeostatic. Instead, we find that α-ketoglutarate, which accumulates in nitrogen limitation, directly blocks glucose uptake by inhibiting enzyme I, the first step of the sugar-phosphoenolpyruvate phosphotransferase system (PTS). This inhibition enables rapid modulation of glycolytic flux without marked changes in the concentrations of glycolytic intermediates by simultaneously altering import of glucose and consumption of the terminal glycolytic intermediate phosphoenolpyruvate. Quantitative modeling shows that this previously unidentified regulatory connection is, in principle, sufficient to coordinate carbon and nitrogen utilization.
authors: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
authors: Christopher D
authors: CD
authors: Doucette
authors: David J
authors: DJ
authors: Schwab
authors: Ned S
authors: NS
authors: Wingreen
authors: Joshua D
authors: JD
authors: Rabinowitz
mesh_terms: D018533:Biomass; D002244:Carbon / Q000737:chemistry / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D005947:Glucose / Q000037:antagonists & inhibitors / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000737:chemistry / Q000494:pharmacology*; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D007656:Ketoglutaric Acids; D002244:Carbon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose; D009584:Nitrogen