title: Gene transfer using micellar nanovectors inhibits corneal neovascularization in vivo.
abstract: OBJECTIVE
  The efficacy of gene therapy using nonviral vector based on polyplex micelle has been studied against corneal neovascularization in mice.
  
  
  METHODS
  A block copolymer, poly(ethylene glycol) (PEG)-block-polycation carrying ethylenediamine units in the side chain (PEG-b-P[Asp(DET)]), was prepared. PEG-b-P[Asp(DET)] formed a polyplex micelle through the polyion complex formation with plasmid DNA. To evaluate in vivo gene transfer efficiency, PEG-b-P[Asp(DET)] micelle was injected into the subconjunctival space of mice, and the expression of the reporter gene was assessed. Furthermore, mouse corneal neovascularization models were treated with the PEG-b-P[Asp(DET)] polyplex micelle containing expression plasmid vector of soluble vascular endothelial growth factor receptor 1 (sflt-1).
  
  
  RESULTS
  Subconjunctival injection of the PEG-b-P[Asp(DET)] polyplex micelle containing a reporter gene showed prolonged gene expression with low cytotoxicity. Also, gene transfer into subconjunctival space by the polyplex micelle containing sflt-1 plasmid showed significant inhibition of corneal neovascularization in mice.
  
  
  CONCLUSIONS
  Nonviral gene therapy using PEG-b-P[Asp(DET)] polyplex micelle may have potential for safe and effective therapeutic treatment of corneal neovascularization.
authors: Department of Ophthalmology, University of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan. akagi-tky@umin.ac.jp
authors: Aya
authors: A
authors: Iriyama
authors: Tomohiko
authors: T
authors: Usui
authors: Yasuo
authors: Y
authors: Yanagi
authors: Shiro
authors: S
authors: Amano
authors: Makoto
authors: M
authors: Oba
authors: Kanjiro
authors: K
authors: Miyata
authors: Nobuhiro
authors: N
authors: Nishiyama
authors: Kazunori
authors: K
authors: Kataoka
mesh_terms: D000818:Animals; D016510:Corneal Neovascularization / Q000628:therapy*; D004195:Disease Models, Animal; D005029:Ethylenediamines; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D051379:Mice; D008823:Micelles; D036103:Nanotechnology; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D011092:Polyethylene Glycols; D040262:Receptors, Vascular Endothelial Growth Factor / Q000008:administration & dosage; D014162:Transfection / Q000379:methods
chemical_list: D005029:Ethylenediamines; D008823:Micelles; D011092:Polyethylene Glycols; C031234:ethylenediamine; D040262:Receptors, Vascular Endothelial Growth Factor