title: Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria.
abstract: A practical antibiotics-free plasmid expression system in cyanobacteria was developed by using the complementation of cyanobacterial recA null mutation with the EscherichiacolirecA gene on the plasmid. This system was applied to the production of polyhydroxyalkanoate (PHA), a biodegradable plastic, and the transgenic cyanobacteria stably maintained the pha genes for PHA production in the antibiotics-free medium, and accumulated up to 52% cell dry weight of PHA.
authors: Toray Research Center, Inc., 6-10-1 Tebiro, Kamakura, Kanagawa 248-8555, Japan.
authors: Hideo
authors: H
authors: Akiyama
authors: Hiroshi
authors: H
authors: Okuhata
authors: Takuo
authors: T
authors: Onizuka
authors: Shozo
authors: S
authors: Kanai
authors: Masahiko
authors: M
authors: Hirano
authors: Satoshi
authors: S
authors: Tanaka
authors: Ken
authors: K
authors: Sasaki
authors: Hitoshi
authors: H
authors: Miyasaka
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000737:chemistry; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D010969:Plastics; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D011938:Rec A Recombinases / Q000235:genetics; D046940:Synechococcus / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D010969:Plastics; D054813:Polyhydroxyalkanoates; D002245:Carbon Dioxide; D011938:Rec A Recombinases