title: Screening of peptides bound to breast cancer stem cell specific surface marker CD44 by phage display.
abstract: CD44, a cancer-associated membrane glycoprotein involved in cell adhesion and tumor progression, has been implicated as a cancer stem cell antigen in several cancers including breast cancer. If the detection sensitivity of CD44 as an early marker for cancer could be improved, this would have important clinical applications. As compared with early stage treatments of other kinds of cancer, treatment of breast cancer is more likely to results in positive outcomes, so this early detection is crucial. Therefore, CD44 is a potential diagnostic target for cancer detection. Herein, we have used a peptide library to screen novel diverse peptides that bind to CD44 with high affinity and characterized the specific binding of these peptides. Our work provides a basis to develop novel diagnostic peptides which may replace antibodies as CD44 detection probes.
authors: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
authors: Hye-Yeon
authors: HY
authors: Park
authors: Kyoung-Jin
authors: KJ
authors: Lee
authors: Su-Jae
authors: SJ
authors: Lee
authors: Moon-Young
authors: MY
authors: Yoon
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D000939:Epitopes; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D000939:Epitopes; D018960:Hyaluronan Receptors; D019151:Peptide Library; D010455:Peptides; D011994:Recombinant Proteins; D016650:Fluorescein-5-isothiocyanate