title: Exogenous addition of a C-xylopyranoside derivative stimulates keratinocyte dermatan sulfate synthesis and promotes migration.
abstract: As C-Xyloside has been suggested to be an initiator of glycosaminoglycan (GAG) synthesis, and GAGs such as Dermatan sulfate (DS) are potent enhancers of fibroblast growth factor (FGF)--10 action, we investigated if a C-Xylopyranoside derivative, (C-β-D-xylopyranoside-2-hydroxy-propane, C-Xyloside), could promote DS production by cultured normal human keratinocytes, how this occurs and if C-Xyloside could also stimulate FGF-dependent cell migration and proliferation. C-Xyloside-treated keratinocytes greatly increased secretion of total sulfated GAGs. Majority of the induced GAG was chondroitin sulfate/dermatan sulfate (CS/DS) of which the major secreted GAG was DS. Cells lacking xylosyltransferase enzymatic activity demonstrated that C-Xyloside was able to stimulate GAG synthesis without addition to core proteins. Consistent with the observed increase in DS, keratinocytes treated with C-Xyloside showed enhanced migration in response to FGF-10 and secreted into their culture media GAGs that promoted FGF-10-dependent cellular proliferation. These results indicate that C-Xyloside may enhance epithelial repair by serving as an initiator of DS synthesis.
authors: Division of Dermatology, University of California, San Diego and Veterans Affairs San Diego Health Care System, San Diego, California, United States of America.
authors: Jun
authors: J
authors: Muto
authors: Nandita Natasha
authors: NN
authors: Naidu
authors: Kenshi
authors: K
authors: Yamasaki
authors: Nathalie
authors: N
authors: Pineau
authors: Lionel
authors: L
authors: Breton
authors: Richard L
authors: RL
authors: Gallo
mesh_terms: D000818:Animals; D016466:CHO Cells; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003871:Dermatan Sulfate / Q000096:biosynthesis* / Q000378:metabolism; D051526:Fibroblast Growth Factor 10 / Q000378:metabolism; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation; D010430:Pentosyltransferases / Q000172:deficiency; D000097608:UDP Xylose-Protein Xylosyltransferase
chemical_list: D017077:Culture Media, Conditioned; D003871:Dermatan Sulfate; D051526:Fibroblast Growth Factor 10; D006027:Glycosides; D010430:Pentosyltransferases; D000097608:UDP Xylose-Protein Xylosyltransferase; C496463:FGF10 protein, human