title: Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model.
abstract: OBJECTIVE
  To examine the effects of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on urethral tissue regeneration and sphincter function.
  
  
  METHODS
  Sixteen female Sprague-Dawley rats underwent vaginal distension (VD) for 3 h. Subsequently, green fluorescence protein (GFP)-labeled DFAT cells (1×10(6) in 20 µL saline, DFAT group, n=8) or saline (20 µL, control group, n=8) were injected into paraurethral connective tissue. Two weeks following VD, leak point pressure (LPP) was measured and an immunohistochemical analysis of the urethra was performed to evaluate urethral sphincter regeneration.
  
  
  RESULTS
  The VD model was characterized by atrophy of the urethral sphincter and showed a decrease in LPP. DFAT cell transplantation resulted in a significant improvement of LPP (DFAT group: 37.3±6.4 vs control group: 21.7±5.7 mmHg, P<0.01). Immunohistochemistry revealed that the striated muscle thickness and smooth muscle α-actin-positive area were significantly (P<0.05) larger in the DFAT group than in the control group. DFAT cell transplantation enhanced macrophage accumulation followed by an increased number of cells in the proliferative state. Transplanted DFAT cells were observed in the damaged smooth muscle layer and showed positive staining for smooth muscle α-actin, suggesting conversion into the smooth muscle cell phenotype.
  
  
  CONCLUSIONS
  DFAT cell transplantation promotes sphincter muscle regeneration and improves LPP in the rat VD model.
authors: Department of Urology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo, Japan.
authors: Daisuke
authors: D
authors: Obinata
authors: Taro
authors: T
authors: Matsumoto
authors: Yuichiro
authors: Y
authors: Ikado
authors: Takahiro
authors: T
authors: Sakuma
authors: Koichiro
authors: K
authors: Kano
authors: Noboru
authors: N
authors: Fukuda
authors: Kenya
authors: K
authors: Yamaguchi
authors: Hideo
authors: H
authors: Mugishima
authors: Satoru
authors: S
authors: Takahashi
mesh_terms: D000199:Actins / Q000032:analysis; D017667:Adipocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D000818:Animals; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D017690:Cell Transplantation; D004195:Disease Models, Animal; D005260:Female; D008264:Macrophages / Q000166:cytology; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000033:anatomy & histology / Q000737:chemistry; D054792:Muscle, Striated / Q000033:anatomy & histology / Q000737:chemistry; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D018709:Statistics, Nonparametric; D014521:Urethra / Q000502:physiology*; D014550:Urinary Incontinence, Stress / Q000628:therapy
chemical_list: D000199:Actins; C498862:Acta2 protein, rat