title: Microalgae as bioreactors for bioplastic production.
abstract: BACKGROUND
  Poly-3-hydroxybutyrate (PHB) is a polyester with thermoplastic properties that is naturally occurring and produced by such bacteria as Ralstonia eutropha H16 and Bacillus megaterium. In contrast to currently utilized plastics and most synthetic polymers, PHB is biodegradable, and its production is not dependent on fossil resources making this bioplastic interesting for various industrial applications.
  
  
  RESULTS
  In this study, we report on introducing the bacterial PHB pathway of R. eutropha H16 into the diatom Phaeodactylum tricornutum, thereby demonstrating for the first time that PHB production is feasible in a microalgal system. Expression of the bacterial enzymes was sufficient to result in PHB levels of up to 10.6% of algal dry weight. The bioplastic accumulated in granule-like structures in the cytosol of the cells, as shown by light and electron microscopy.
  
  
  CONCLUSIONS
  Our studies demonstrate the great potential of microalgae like the diatom P. tricornutum to serve as solar-powered expression factories and reveal great advantages compared to plant based production systems.
authors: LOEWE Research Centre for Synthetic Microbiology, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors: Franziska
authors: F
authors: Hempel
authors: Andrew S
authors: AS
authors: Bozarth
authors: Nicole
authors: N
authors: Lindenkamp
authors: Andreas
authors: A
authors: Klingl
authors: Stefan
authors: S
authors: Zauner
authors: Uwe
authors: U
authors: Linne
authors: Alexander
authors: A
authors: Steinbüchel
authors: Uwe G
authors: UG
authors: Maier
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D020118:Cupriavidus necator / Q000201:enzymology* / Q000235:genetics; D017377:Diatoms / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering; D006885:Hydroxybutyrates / Q000378:metabolism*; D058086:Microalgae / Q000235:genetics / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D000217:Acyltransferases; D000429:Alcohol Oxidoreductases; D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023744:acetoacetyl-CoA reductase; C060970:poly-beta-hydroxybutyrate polymerase