title: Rapid and economic DNA extraction from a single salmon egg for real-time PCR amplification.
abstract: Salmon eggs are common in Japanese sushi and other seafood products; however, certain fish eggs are used as counterfeit salmon eggs which are found in foods and processed products. This study develops a simple, rapid, and cost-effective method for DNA extraction, filtration (FT) and dilution (DL) protocols from a single salmon egg with good DNA quality for real-time PCR amplification. The DNA amount, DNA quality, and real-time PCR performance for different dilutions and different lengths of PCR amplicons were evaluated and compared with the common Qiagen tissue kit (QTK) and Chelex-100-based (CX) protocols. The extracted DNA from a single salmon egg using the FT or DL protocol can be applied in phylogenic research, food authentication and post-marketing monitoring of genetically modified (GM) food products.
authors: Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan. jiyang@mail.nkmu.edu.tw
authors: Jing-Iong
authors: JI
authors: Yang
authors: Hsiao-Yun
authors: HY
authors: Huang
authors: Yii-Cheng
authors: YC
authors: Chou
authors: Chien-Cheng
authors: CC
authors: Chen
authors: Guo-Chi
authors: GC
authors: Lee
authors: Hsueh-Wei
authors: HW
authors: Chang
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D003362:Cost-Benefit Analysis; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D005374:Filtration; D010063:Ovum; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012474:Salmon; D013997:Time Factors
chemical_list: D004247:DNA