title: Isolation and structural confirmation of the oligosaccharides containing α-D-fructofuranoside linkages isolated from fermented beverage of plant extracts.
abstract: Fermented beverage of plant extracts was prepared from the extracts of approximately 50 types of vegetables and fruits. Natural fermentation was carried out mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp. and Pichia spp.). Two oligosaccharides containing an α-fructofuranoside linkage were detected in this beverage and isolated using carbon-Celite column chromatography and preparative HPLC. The structural confirmation of the saccharides was determined by methylation analysis, MALDI-TOF-MS, and NMR measurements. These saccharides were identified as α-D-fructofuranosyl-(2→6)-D-glucopyranose, which was isolated from a natural source for the first time, and a novel saccharide β-D-fructopyranosyl-(2→6)-α-D-fructofuranosyl-(2↔1)-α-D-glucopyranoside.
authors: General Institute of Ohtakakohso Co., Ltd, 1-22-10 Sakura, Otaru 047-0193, Japan. oelab@ohtakakohso.co.jp
authors: Hideki
authors: H
authors: Okada
authors: Eri
authors: E
authors: Fukushi
authors: Akira
authors: A
authors: Yamamori
authors: Naoki
authors: N
authors: Kawazoe
authors: Shuichi
authors: S
authors: Onodera
authors: Jun
authors: J
authors: Kawabata
authors: Norio
authors: N
authors: Shiomi
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D005285:Fermentation; D007934:Leuconostoc; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D004718:Saccharomycetales; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014312:Trisaccharides / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004187:Disaccharides; D010936:Plant Extracts; D014312:Trisaccharides