title: Analysis of a second-generation tetracycline antibiotic minocycline in human plasma by LC-MS/MS.
abstract: A simple, selective and sensitive LC-MS/MS assay has been developed for the determination of minocycline in human plasma. Plasma samples were prepared by protein precipitation, followed by chromatographic separation on a HyPURITY™ C8 (100 mm × 4.6 mm, 5 µm) column under isocratic conditions. The LOD and LLOQ of the method were 0.50 and 20.0 ng/ml, respectively. The intra-batch and inter-batch precision (% coefficient of variation) across quality control levels was less than 8.0%. For a set of incurred samples the percentage change in concentration was within ± 9.0%. The method was successfully applied to a bioequivalence study of 135 mg minocycline tablet formulation in 14 healthy Indian males. The reproducibility in the measurement of study data was demonstrated by incurred sample reanalysis.
authors: Chemistry Department, Pramukh Swami Science and H.D. Patel Arts College, Sarva Vidyalaya Campus, Kadi, Gujarat, India.
authors: Dinesh S
authors: DS
authors: Patel
authors: Naveen
authors: N
authors: Sharma
authors: Mukesh C
authors: MC
authors: Patel
authors: Bhavin N
authors: BN
authors: Patel
authors: Pranav S
authors: PS
authors: Shrivastav
authors: Mallika
authors: M
authors: Sanyal
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008911:Minocycline / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D008911:Minocycline