title: Simultaneous determination of six phenolic constituents of Danshen injection in rat plasma by LC-ESI-MS and its application to a pharmacokinetic study.
abstract: Salvianolic acid A, salvianolic acid B, danshensu, protocatechuic aldehyde, rosmarinic acid and lithospermic acid are the six major active constituents in Danshen injection. In this study, a rapid, sensitive and specific liquid chromatographic-electrospray ionization-mass spectrometry method for the simultaneous quantitative determination of these compounds in rat plasma was developed. After a single step of liquid-liquid extraction with ethyl acetate, they were eluted by a Hypersil C18 column (5 µm, i.d. 4.6 × 200 mm) within 4 min with a mobile phase consisting of acetonitrile and 0.1% formic acid water solution (35:65, v/v). The assay was linear in the concentration range of 0.05-10 µg mL(-1). Absolute recoveries were above 60%. The precisions and accuracies determined within three consecutive days were within acceptable limits. The method was successfully applied to a pharmacokinetic study in rats after an intravenous administration of Danshen injection.
authors: China Pharmaceutical University, Jiangsu, People's Republic of China.
authors: Di
authors: D
authors: Zhao
authors: De-en
authors: DE
authors: Han
authors: Ning
authors: N
authors: Li
authors: Yang
authors: Y
authors: Lu
authors: Ting-ting
authors: TT
authors: Li
authors: Suo-ye
authors: SY
authors: Yang
authors: Jia-ke
authors: JK
authors: He
authors: Xi-jing
authors: XJ
authors: Chen
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000097:blood / Q000493:pharmacokinetics; D001572:Benzofurans / Q000097:blood / Q000493:pharmacokinetics; D002109:Caffeic Acids / Q000097:blood / Q000493:pharmacokinetics; D002396:Catechols / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D002934:Cinnamates / Q000097:blood / Q000493:pharmacokinetics; D053630:Depsides / Q000097:blood / Q000493:pharmacokinetics; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D007773:Lactates / Q000097:blood / Q000493:pharmacokinetics; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D028321:Plant Preparations / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000097123:Rosmarinic Acid
chemical_list: D001547:Benzaldehydes; D001572:Benzofurans; D002109:Caffeic Acids; D002396:Catechols; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D007773:Lactates; D028321:Plant Preparations; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; C066201:salvianolic acid A; C076944:salvianolic acid B