title: A nonsynonymous TNFRSF11A variation increases NFκB activity and the severity of Paget's disease.
abstract: Mutations in the SQSTM1 gene were identified as a common cause of Paget's disease of bone (PDB) but experimental evidence demonstrated that SQSTM1 mutation is not sufficient to induce PDB in vivo. Here, we identified two nonsynonymous single nucleotide polymorphisms (SNPs) (C421T, H141Y and T575C, V192A) in the TNFRSF11A gene, associated with PDB and with the severity of phenotype in a large population of 654 unrelated patients that were previously screened for SQSTM1 gene mutations. The largest effect was found for the T575C variant, yielding an odds ratio of 1.29 (p = 0.003), with the C allele as the risk allele. Moreover, an even more significant p-value (p = 0.0002) was observed in the subgroup of patients with SQSTM1 mutation, with an odds ratio of 1.71. Interestingly, patients with the C allele also showed an increased prevalence of polyostotic disease (68%, 53%, and 51% in patients with CC, CT, and TT genotypes, respectively; p = 0.01), as well as an increased number of affected skeletal sites (2.9, 2.5, and 2.0 in patients with CC, CT, and TT genotypes, respectively, p = 0.008). These differences increased when analyses were restricted to cases with SQSTM1 mutation. In human cell lines, cotrasfection with mutated SQSTM1 and TNFRSF11A(A192) produced a level of activation of NFκB signaling greater than cotrasfection with wild-type SQSTM1 and TNFRSF11A(V192), confirming genetics and clinical evidences. These results provide the first evidence that genetic variation within the OPG/RANK/RANKL system influences the severity of PBD in synergistic action with SQSTM1 gene mutations.
authors: Institute of Genetics and Biophysics Adriano Buzzati-Traverso, National Research Council of Italy, Naples, Italy. gianfrancesco@igb.cnr.it
authors: Fernando
authors: F
authors: Gianfrancesco
authors: Domenico
authors: D
authors: Rendina
authors: Marco
authors: M
authors: Di Stefano
authors: Alessandra
authors: A
authors: Mingione
authors: Teresa
authors: T
authors: Esposito
authors: Daniela
authors: D
authors: Merlotti
authors: Salvatore
authors: S
authors: Gallone
authors: Sara
authors: S
authors: Magliocca
authors: Alice
authors: A
authors: Goode
authors: Daniela
authors: D
authors: Formicola
authors: Giovanna
authors: G
authors: Morello
authors: Robert
authors: R
authors: Layfield
authors: Annalisa
authors: A
authors: Frattini
authors: Gianpaolo
authors: G
authors: De Filippo
authors: Ranuccio
authors: R
authors: Nuti
authors: Mark
authors: M
authors: Searle
authors: Pasquale
authors: P
authors: Strazzullo
authors: Giancarlo
authors: G
authors: Isaia
authors: Giuseppe
authors: G
authors: Mossetti
authors: Luigi
authors: L
authors: Gennari
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D016022:Case-Control Studies; D002460:Cell Line; D017124:Conserved Sequence / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D017930:Genes, Reporter; D056726:Genetic Association Studies; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D010001:Osteitis Deformans / Q000235:genetics*; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics*; D012720:Severity of Illness Index
chemical_list: D050505:Mutant Proteins; D016328:NF-kappa B; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; D008156:Luciferases