title: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting.
abstract: We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.
authors: Vector Borne Disease Training Center, Pra Budhabat, Saraburi, Thailand. grphat@yahoo.com
authors: Jeeraphat
authors: J
authors: Sirichaisinthop
authors: Sureemas
authors: S
authors: Buates
authors: Risa
authors: R
authors: Watanabe
authors: Eun-Taek
authors: ET
authors: Han
authors: Wachira
authors: W
authors: Suktawonjaroenpon
authors: Somporn
authors: S
authors: Krasaesub
authors: Satoru
authors: S
authors: Takeo
authors: Takafumi
authors: T
authors: Tsuboi
authors: Jetsumon
authors: J
authors: Sattabongkot
mesh_terms: D006801:Humans; D008288:Malaria / Q000175:diagnosis*; D012680:Sensitivity and Specificity