title: Recognition of linear stress fibers based on Hough transform.
abstract: OBJECTIVE
  To present an algorithm based on Hough transform for recognition and extraction of linear stress fibers formed on exposure to lysophosphatidic acid (LPA).
  
  
  METHODS
  A ridge set of head points with lower shoulders is calculated, followed by a thinning process shrinking long, narrow regions to regions of single pixel thickness, then converted into a rectangular map whose value is the number of regional points in the path of a straight line at the angle and intercept determined by two coordinates. The location of the maximum in the map is sought, and the corresponding line with an unlimited length is constructed from the paired coordinates. We removed the line before repeating the process for the next longest straight line, continuing until all lines with reasonable lengths are extracted.
  
  
  RESULTS
  Application of the algorithm to the stress fiber images of DOV13 cells stained with Texas red-phalloidin on LPA and AG1478 demonstrates close matches between stress fibers in the original images and linear lines.
  
  
  CONCLUSIONS
  An algorithm for recognition of linear stress fibers formed on exposure to LPA is described and applications to stress fiber images using DOV13 cells with Texas red-phalloidin staining are demonstrated.
authors: Department of Pathology, Mount Sinai School of Medicine, New York, New York 10029, USA. haishan.wu@mssm.edu
authors: Hai-Shan
authors: HS
authors: Wu
authors: Feng-Qiang
authors: FQ
authors: Wang
authors: Yoel
authors: Y
authors: Smicun
authors: David
authors: D
authors: Fishman
mesh_terms: D000465:Algorithms; D045744:Cell Line, Tumor; D006801:Humans; D008246:Lysophospholipids / Q000737:chemistry*; D010590:Phalloidine / Q000737:chemistry; D022502:Stress Fibers / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D008246:Lysophospholipids; D010590:Phalloidine; C032881:lysophosphatidic acid