title: Protein glycosylation and its impact on biotechnology.
abstract: Glycosylation is a post-translational modification that is of paramount importance in the production of recombinant pharmaceuticals as most recombinantly produced therapeutics are N- and/or O-glycosylated. Being a cell-system-dependent process, it also varies with expression systems and growth conditions, which result in glycan microheterogeneity and macroheterogeneity. Glycans have an effect on drug stability, serum half-life, and immunogenicity; it is therefore important to analyze and optimize the glycan decoration of pharmaceuticals. This review summarizes the aspects of protein glycosylation that are of interest to biotechnologists, namely, biosynthesis and biological relevance, as well as the tools to optimize and to analyze protein glycosylation.
authors: Markus
authors: M
authors: Berger
authors: Matthias
authors: M
authors: Kaup
authors: Veronique
authors: V
authors: Blanchard
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D011134:Polysaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; D011506:Proteins