title: CTX-M-14 type β-Lactamase producing Escherichia coli isolated from hospitalized patients in Tunisia.
abstract: Escherichia coli (E.coli) with a CTX-M resistance phenotype was selected from hospitalized patients in a university Hospital of Tunisia between January 2010 and June 2010. PCR analysis and sequencing demonstrated that it harboured CTX-M-14 β-lactamase. Characterization of the regions surrounding the bla(CTX-M-14) showed the ISEcp1 elements located in the upstream region of the bla gene. PFGE and multilocus sequence typing revealed two different types which corresponds to sequence types ST38 complex and ST131. These results reinforce the potential for spreading of this gene among E. coli clinical strains in the coming years in Tunisia.
authors: Laboratoire de microbiologie, Hôpital militaire de Tunis, Monfleury, Tunisia. hella_lahlaoui@yahoo.fr
authors: Hella
authors: H
authors: Lahlaoui
authors: Mohamed
authors: M
authors: Ben Moussa
mesh_terms: D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification; D006760:Hospitalization; D006801:Humans; D016133:Polymerase Chain Reaction; D014416:Tunisia; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: C456080:beta-lactamase CTX-M-14; D001618:beta-Lactamases