title: Smallest genetically encoded phosphorylation status sensor.
abstract: Do not disturb! As an environment-sensitive fluorescent amino acid, L-(7-hydroxycoumarin-4-yl) ethylglycine (7HC) is genetically encodable in E. coli containing a suppressor tRNA and a cognate 7HC-specific tRNA synthetase. Its incorporation into a full-length protein allows the detection of a nearby phosphorylation event with minimum influence on protein structures and functions, thereby serving as the smallest phosphorylation sensor.
authors: Institute for Bioscience and Biotechnology Research and Department of Chemistry and Biochemistry, University of Maryland College Park, 9600 Gudelsky Drive, Rockville, MD 20850, USA. sli@umd.edu
authors: Shuwei
authors: S
authors: Li
mesh_terms: D008958:Models, Molecular; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D011506:Proteins