title: Neuroprotective iridoid glycosides from Cornus officinalis fruits against glutamate-induced toxicity in HT22 hippocampal cells.
abstract: The methanolic extract of the fruits of Cornus officinalis S et Z. (Cornaceae) showed the significant neuroprotective activity against glutamate-induced toxicity in HT22 hippocampal cells. Chemical profile of n-BuOH fraction of the methanolic extract of C. officinalis fruits, which showed the most potent activity, was established using HPLC-diode array detector-electrospray-MS (HPLC-DAD-ESI-MS). Through bioactivity-guided isolation, five iridoid glycosides including one new compound, 7-O-butylmorroniside (1), loganin (2), morroniside (3), 7R-O-methylmorroniside (4), 7S-O-methylmorroniside (5) were isolated from the n-BuOH fraction. The protective activities of the isolated compounds, themselves, were not statistically significant. However, the hydrolyzed products of compounds 1, 4 and 5 significantly protected glutamate-injured HT22 cells up to 78±2.2%, 60±3.2% and 59±2.5% of non-treated control, respectively.
authors: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Eun Ju
authors: EJ
authors: Jeong
authors: Tae Bum
authors: TB
authors: Kim
authors: Heejung
authors: H
authors: Yang
authors: So Young
authors: SY
authors: Kang
authors: Sun Yeou
authors: SY
authors: Kim
authors: Sang Hyun
authors: SH
authors: Sung
authors: Young Choong
authors: YC
authors: Kim
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D018698:Glutamic Acid / Q000009:adverse effects*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D006868:Hydrolysis; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology*; D000432:Methanol / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D006027:Glycosides; D039823:Iridoids; D018696:Neuroprotective Agents; D010936:Plant Extracts; C488401:morroniside; D018698:Glutamic Acid; C059516:loganin; D000432:Methanol