title: Effects of hormone agonists on Sf9 cells, proliferation and cell cycle arrest.
abstract: Methoxyfenozide and methoprene are two insecticides that mimic the action of the main hormones involved in the control of insect growth and development, 20-hydroxyecdysone and juvenile hormone. We investigated their effect on the Spodoptera frugiperda Sf9 cell line. Methoxyfenozide was more toxic than methoprene in cell viability tests and more potent in the inhibition of cellular proliferation. Cell growth arrest occurred in the G2/M phase after a methoprene treatment and more modestly in G1 after methoxyfenozide treatment. Microarray experiments and real-time quantitative PCR to follow the expression of nuclear receptors ultraspiracle and ecdysone receptor were performed to understand the molecular action of these hormone agonists. Twenty-six genes were differentially expressed after methoxyfenozide treatment and 55 genes after methoprene treatment with no gene in common between the two treatments. Our results suggest two different signalling pathways in Sf9 cells.
authors: Institut National de la Recherche Agronomique, UMR 1301 Interactions Biotiques et Santé Végétale, Centre National de la Recherche Scientifique, UMR 6243, Université de Nice Sophia Antipolis, Sophia-Antipolis, France.
authors: Maeva
authors: M
authors: Giraudo
authors: Jérôme
authors: J
authors: Califano
authors: Frédérique
authors: F
authors: Hilliou
authors: Trang
authors: T
authors: Tran
authors: Nathalie
authors: N
authors: Taquet
authors: René
authors: R
authors: Feyereisen
authors: Gaëlle
authors: G
authors: Le Goff
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D017344:Genes, Insect / Q000235:genetics; D006834:Hydrazines / Q000633:toxicity; D007306:Insecticides / Q000633:toxicity; D007605:Juvenile Hormones / Q000819:agonists* / Q000633:toxicity; D008726:Methoprene / Q000633:toxicity; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D018411:Spodoptera / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics
chemical_list: D006834:Hydrazines; D007306:Insecticides; D007605:Juvenile Hormones; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; C406712:methoxyfenozide; D008726:Methoprene