title: Microcantilevers modified by specific peptide for selective detection of trimethylamine.
abstract: We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.
authors: Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, People's Republic of China.
authors: Xin
authors: X
authors: Huang
authors: Mingfu
authors: M
authors: Li
authors: Xiaohe
authors: X
authors: Xu
authors: Hongjun
authors: H
authors: Chen
authors: Hai-Feng
authors: HF
authors: Ji
authors: Shuifang
authors: S
authors: Zhu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D008744:Methylamines / Q000032:analysis*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D010455:Peptides / Q000737:chemistry*; D025941:Protein Interaction Mapping / Q000295:instrumentation*
chemical_list: D008744:Methylamines; D010455:Peptides; C023336:trimethylamine