title: Rapid multitarget immunomagnetic separation through programmable DNA linker displacement.
abstract: Immunomagnetic separation has become an essential tool for high-throughput and low-cost isolation of biomolecules and cells from heterogeneous samples. However, as magnetic selection is essentially a "black-and-white" assay, its application has been largely restricted to single-target and single-parameter studies. To address this issue, we have developed an immunomagnetic separation technology that can quickly sort multiple targets in high yield and purity using selectively displaceable DNA linkers. We envision that this technology will be readily adopted for experiments requiring high-throughput selection of multiple targets or further adapted for selection of a single target based on multiple surface epitopes.
authors: Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
authors: Christine E
authors: CE
authors: Probst
authors: Pavel
authors: P
authors: Zrazhevskiy
authors: Xiaohu
authors: X
authors: Gao
mesh_terms: D000906:Antibodies / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018189:Immunomagnetic Separation / Q000379:methods*; D008280:Magnetics; D013997:Time Factors
chemical_list: D000906:Antibodies; D004247:DNA