title: Raman spectroscopy of individual monocytes reveals that single-beam optical trapping of mononuclear cells occurs by their nucleus.
abstract: We show that laser-tweezers Raman spectroscopy of eukaryotic cells with a significantly larger diameter than the tight focus of a single beam laser trap leads to optical trapping of the cell by its optically densest part, i.e. typically the cell's nucleus. Raman spectra of individual optically trapped monocytes are compared with location-specific Raman spectra of monocytes adhered to a substrate. When the cell's nucleus is stained with a fluorescent live cell stain, the Raman spectrum of the DNA-specific stain is observed only in the nucleus of individual monocytes. Optically trapped monocytes display the same behavior. We also show that the Raman spectra of individual monocytes exhibit the characteristic Raman signature of cells that have not yet fully differentiated and that individual primary monocytes can be distinguished from transformed monocytes based on their Raman spectra. This work provides further evidence that laser tweezers Raman spectroscopy of individual cells provides meaningful biochemical information in an entirely nondestructive fashion that permits discerning differences between cell types and cellular activity.
authors: NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA 95817, USA.
authors: Samantha
authors: S
authors: Fore
authors: James
authors: J
authors: Chan
authors: Douglas
authors: D
authors: Taylor
authors: Thomas
authors: T
authors: Huser