title: Phenotypic and molecular characterization of Arcanobacterium haemolyticum isolated from clinical samples.
abstract: The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological, biochemical, and antigenic tests. Species identification was confirmed by amplification and sequencing of the 16S rDNA gene. Antimicrobial susceptibility testing was determined by microdilution following the Clinical and Laboratory Standards Institute recommendations for coryneform bacteria. Genetic diversity was evaluated using BOX-PCR and pulsed-field gel electrophoresis. Eighty percent of the isolates had an identical BOX-PCR pattern, suggesting the spread of a single clone. The present report provides extensive information on the phenotypic and genotypic characterization of A. haemolyticum.
authors: Servicio de Microbiología. Hospital Universitario Marqués de Valdecilla-IFIMAV, Santander, Spain.
authors: Celia
authors: C
authors: García-de-la-Fuente
authors: Carlos
authors: C
authors: Ruiz De Alegría
authors: María Eliecer
authors: ME
authors: Cano
authors: Ana Belén
authors: AB
authors: Campo
authors: Fabián
authors: F
authors: Unda
authors: Jesús
authors: J
authors: Agüero
authors: Luis
authors: L
authors: Martínez-Martínez
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D055667:Arcanobacterium / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S