title: Suppression of the invasion and migration of cancer cells by SERPINB             family genes and their derived peptides.
abstract: Apart from SERPINB2 and SERPINB5, the roles of the remaining 13 members             of the human SERPINB family in cancer metastasis are still unknown. In the present             study, we demonstrated that most of these genes are differentially expressed in             tumor tissues compared to matched normal tissues from lung or breast cancer patients.             Overexpression of each SERPINB gene effectively suppressed the invasiveness and             motility of malignant cancer cells. Among all of the genes, the SERPINB1, SERPINB5             and SERPINB7 genes were more potent, and the inhibitory effect was further enhanced             by co-expression of any two of them. In addition, single treatment of the synthetic             peptides corresponding to the P5-P5' sequences of the reactive center loop (RCL)             of SERPINB1, SERPINB5 or SERPINB7 markedly suppressed the invasive and migratory             properties of the cancer cells in a dose-dependent manner. More significantly,             combination treatment of these peptides in cancer cells further improved the suppressive             effect by 20-40%. Here, we determined the expression of all SERPINB family members             in lung and breast cancer patients, and identified those members with potent inhibitory             ability toward invasion and migration, and designed RCL-derived peptides to suppress             the malignancy of cancer cells. Forced re-expression of these anti-invasive SERPINB             genes or application of the SERPINB RCL-peptides may provide a reasonable strategy             against lethal cancer metastasis.
authors: National Institute of Cancer Research, National Health Research Institutes, Miaoli, Taiwan, ROC. rhchou@gmail.com
authors: Ruey-Hwang
authors: RH
authors: Chou
authors: Hui-Chin
authors: HC
authors: Wen
authors: Wei-Guang
authors: WG
authors: Liang
authors: Sheng-Chieh
authors: SC
authors: Lin
authors: Hsiao-Wei
authors: HW
authors: Yuan
authors: Cheng-Wen
authors: CW
authors: Wu
authors: Wun-Shaing Wayne
authors: WS
authors: Chang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000235:genetics*
chemical_list: D015843:Serpins