title: Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC.
abstract: A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance.
authors: Department of Microbiological Diagnostics, The National Reference Laboratory for Enteropathogenic Bacteria, Unit of Gastrointestinal Infections, Statens Serum Institut, Ørestads Boulevard 5, 2300 Copenhagen S, Denmark. SPN@ssi.dk
authors: Søren
authors: S
authors: Persson
authors: Joan N
authors: JN
authors: Jensen
authors: Katharina E P
authors: KE
authors: Olsen
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016360:Clostridioides difficile / Q000235:genetics* / Q000472:pathogenicity; D004768:Enterotoxins / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012097:Repressor Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004768:Enterotoxins; D012097:Repressor Proteins; C105615:TcdC protein, Clostridium difficile; D037521:Virulence Factors; D036002:ADP Ribose Transferases