title: [The regulation of T3 on maldeveloped gonocyte (Go) and expression of NCAM in flutamide-induced cryptorchidism rat].
abstract: OBJECTIVE
  To investigate the regulation of T3 on the maldeveloped gonocytes (Go) and the expression of NCAM in Flutamide-induced cryptorchidism SD rat.
  
  
  METHODS
  Postnatal day (PD) 1 and PD20 cryptorchidism SD rat induced by Flu were treated with 15 microg/(kg x d) T3 by subcutaneous injections. Incidence of the cryptorchidism, histology were evaluated. And the expression of NCAM was detected by immunohistochemisty and RT-PCR.
  
  
  RESULTS
  The incidence of cryptorchidism in the group of Flu-induced offsprings treated by 15 microg/(kg x d) T3 from PD1 was 25% (8/32), which was lower than that of Flu group 40.9% (9/22). No Go and NCAM expression were observed in the seminiferous tubules. NCAM mRNA expression in Flu-induced PD13, PD20 testes was up-regulated when compared with control (P < 0.01, P < 0.05), while after treated with 15 microg/ (kg x d) T3 the NCAM expression was significantly down-regulated compared to that of non T3 treated group (P < 0.01), and was not significantly different from that of control (P > 0.05).
  
  
  CONCLUSIONS
  The incidence of Flu-induced cryptorchidism can be decreased by T3 treatment after birth. T3 may contribute to the down-regulation of the NCAM expression, and reduction or elimination of the remainded gonocytes in crytorcidism seminiferous tubules.
authors: Ministry of Education Key Laboratory of Child Development and Disorders, Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors: Chao
authors: C
authors: Ma
authors: Da-Wei
authors: DW
authors: He
authors: Yong-Chao
authors: YC
authors: Jiang
authors: Dan
authors: D
authors: Zhao
authors: Wen-Fei
authors: WF
authors: He
authors: Guang-Hui
authors: GH
authors: Wei
mesh_terms: D000818:Animals; D003456:Cryptorchidism / Q000139:chemically induced / Q000378:metabolism*; D005485:Flutamide; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013093:Spermatogonia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D014284:Triiodothyronine; D005485:Flutamide