title: Conversions at C-30 of glycyrrhetinic acid and their impact on antitumor activity.
abstract: The extracts of the roots of licorice have been used in traditional and folk medicine to treat a broad variety of maladies. The main ingredient of these extracts is glycyrrhicinic acid. Its aglycon, glycyrrhetinic acid, has many biological activities, among them a pronounced cytotoxicity against tumor cells. In this study we varied glycyrrhetinic acid at position C-30 to get "simple" derivatives, for example esters, amides and a nitrile. The influence of these changes on the cytotoxic activity is noteworthy and was determined by a colorimetric sulphorhodamine B test using 7 human tumor cell lines and mouse embryonic fibroblasts (NIH3T3) for comparison. A Trypan blue test as well as an acridine orange/ethidium bromide test was used to discover the ability of the compounds to induce apoptosis.
authors: Martin-Luther-Universität Halle-Wittenberg, Bereich Organische Chemie, Halle (Saale), Germany. rene.csuk@chemie.uni-halle.de
authors: René
authors: R
authors: Csuk
authors: Stefan
authors: S
authors: Schwarz
authors: Bianka
authors: B
authors: Siewert
authors: Ralph
authors: R
authors: Kluge
authors: Dieter
authors: D
authors: Ströhl
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000379:methods; D004354:Drug Screening Assays, Antitumor; D006034:Glycyrrhetinic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008519:Medicine, Traditional; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000188:drug therapy*; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D012235:Rhodamines; C022027:lissamine rhodamine B; D006034:Glycyrrhetinic Acid