title: Effects of suxiao jiuxin pill (see test) on oxidative stress and inflammatory response in rats with experimental atherosclerosis.
abstract: OBJECTIVE
  To observe the preventive role of Suxiao Jiuxin Pill (see text) on atherosclerosis (AS) and to probe into the mechanism in the atherosclerosis rat model.
  
  
  METHODS
  The AS rat model was established by a high fat diet and a large dose of calcium (vitamin D3, 0.6 million U/kg, i.p, once). Sixty healthy male adult Sprague-Dawlay (SD) rats were randomly divided into 6 groups, a normal control group (N), a model group (M), a SX low dose group (SXL), a SX middle dose group (SXM), a SX high dose group (SXH), and an atorvastatin group (ATO) (n = 10 in each group). The rats in the treatment groups were given with the specific drugs from the first day by oral administration, and the normal control group and the model group were given with normal saline for 12 weeks. Afterwards, the content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and the content of oxidized low density lipoprotein (ox-LDL) in the serum were detected. In addition, the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) proteins were tested by Western-blot method.
  
  
  RESULTS
  The serum ox-LDL and MDA level significantly decreased, SOD activity increased in the SX middle, high dose groups and the atorvastatin group compared to the model group (all P < 0.05). While the expression of PPARgamma and NF-kappab proteins significantly decreased in the SX low, middle, high dose groups and the atorvastatin group compared to the model group (all P < 0.01), with the best effect in the SX high dose group .These results indicate that SX could elevate the activity of serum SOD, decrease serum level of MDA and ox-LDL, and reduce the expression of PPARgamma and NF-kappaB proteins.
  
  
  CONCLUSIONS
  SX plays an important role in anti-inflammation and inhibition of oxidative stress, which possibly are the mechanism of its preventing and treating atherosclerosis.
authors: Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300054, China. lichunshen@126.com
authors: Chun-Shen
authors: CS
authors: L i
authors: Zhu-Qiu
authors: ZQ
authors: Qu
authors: Sha-Sha
authors: SS
authors: Wang
authors: Xu-Wen
authors: XW
authors: Hao
authors: Xiu-Qin
authors: XQ
authors: Zhang
authors: Jing
authors: J
authors: Guan
authors: Fei
authors: F
authors: Han
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D007249:Inflammation / Q000188:drug therapy*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D016328:NF-kappa B / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D047495:PPAR gamma / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D016328:NF-kappa B; D047495:PPAR gamma; C099252:oxidized low density lipoprotein; C543119:suxiao jiuxinwan; D008315:Malondialdehyde; D013482:Superoxide Dismutase