title: Studies on the expression of fibroblast growth factor-2 from odontoblast-like cells.
abstract: BACKGROUND
  The goal of this study was to evaluate the mechanism involved in the expression of fibroblast growth factor-2 (FGF-2) by odontoblast-like cells (ODs) stimulated by lipopolysaccharide (LPS) via p42/44, p38, and PI3K.
  
  
  METHODS
  ODs (MDPC-23) were stimulated with LPS for 1, 6, and 24 hours. The FGF-2 expression was evaluated by reverse transcriptase-polymerase chain reaction and protein production by Western blot analysis. Cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD98059, PD), p38 inhibitor (SB202190, SB), or PI3K inhibitor (wortmannin, Wort) and then stimulated with LPS (0.1 μg/mL) for 1 hour.
  
  
  RESULTS
  LPS-stimulated ODs express FGF-2 in concentrations at 0.1, 1, 10, and 100 μg/mL after 1 hour. DEX and MK were able to inhibit FGF-2 mRNA expression. PD, SB, and Wort also decreased expression.
  
  
  CONCLUSIONS
  LPS-induced FGF-2 mRNA expression on ODs occurs via leukotriene production or cytokine and/or chemokine production activating p42/44, p38, and PI3K pathway. The data suggest that FGF-2 released by ODs might act as modulators of immune response mainly in the tissue repair.
authors: Department of Basic Sciences, School of Dentistry of Araçatuba, Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil. shpoliv@foa.unesp.br
authors: Sandra H P
authors: SH
authors: Oliveira
authors: Vanessa A C
authors: VA
authors: Santos
mesh_terms: D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D004789:Enzyme Activation; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology*; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D015289:Leukotrienes / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002722:Chlorobenzenes
chemical_list: D018925:Chemokines; D016207:Cytokines; D003907:Dexamethasone; D016222:Fibroblast Growth Factor 2; D005938:Glucocorticoids; D007211:Indoles; D015289:Leukotrienes; D008070:Lipopolysaccharides; D016859:Lipoxygenase Inhibitors; D020928:Mitogen-Activated Protein Kinases; C060893:MK-886; D002722:Chlorobenzenes