title: Reengineering of a fluorescent zinc sensor protein yields the first genetically encoded cadmium probe.
abstract: Introduction of a (Cys)(4) metal binding site at the dimerization interface of two fluorescent protein domains yields a chelating FRET sensor protein that shows a 2500-fold selectivity for Cd(2+) over Zn(2+) by taking advantage of their different ionic radii.
authors: Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
authors: Jan L
authors: JL
authors: Vinkenborg
authors: Sander M J
authors: SM
authors: van Duijnhoven
authors: Maarten
authors: M
authors: Merkx
mesh_terms: D001665:Binding Sites; D002104:Cadmium / Q000032:analysis*; D002460:Cell Line; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D002104:Cadmium; D049452:Green Fluorescent Proteins; D015032:Zinc