title: Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.
abstract: We have previously shown that medium-chain triglyceride (MCT) resulted in significantly less body fat mass than long-chain triglyceride (LCT) did in hypertriglyceridimic subjects. The possible mechanism for this was investigated by measuring and analyzing changes in the body fat, blood lipid profile, enzymatic level and activity of hormone-sensitive lipase (HSL) and its mRNA expression, and levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) in white adipose tissue (WAT) of C57BL/6J mice fed for 16 weeks on an MCT or LCT diet. MCT induced lower body weight and body fat, and an improved blood lipid profile than LCT did. The enzymatic level and activity of HSL and its mRNA expression, and the levels of cAMP and PKA were significantly higher in WAT of mice fed with the MCT diet. No significant differences in the levels of lipoprotein lipase and peroxisome proliferator-activated receptor-γ in WAT were apparent between the effects of MCT and LCT. It is concluded that lipolysis by the increased level and activity of HSL, which was induced by the activation of cAMP-dependent PKA in WAT, was partially responsible for the lower fat accumulation in C57BL/6J mice fed with MCT.
authors: Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin, China.
authors: Yinghua
authors: Y
authors: Liu
authors: Changyong
authors: C
authors: Xue
authors: Yong
authors: Y
authors: Zhang
authors: Qing
authors: Q
authors: Xu
authors: Xiaoming
authors: X
authors: Yu
authors: Xinsheng
authors: X
authors: Zhang
authors: Jin
authors: J
authors: Wang
authors: Rongxin
authors: R
authors: Zhang
authors: Xue
authors: X
authors: Gong
authors: Changjiang
authors: C
authors: Guo
mesh_terms: D052438:Adipocytes, White / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects; D047495:PPAR gamma / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D002787:Sterol Esterase / Q000235:genetics* / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D047495:PPAR gamma; D012333:RNA, Messenger; D014280:Triglycerides; D000242:Cyclic AMP; D064429:Fatty Acid Synthases; D017868:Cyclic AMP-Dependent Protein Kinases; D002787:Sterol Esterase; D008071:Lipoprotein Lipase