title: Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction.
abstract: To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.
authors: Division of Community Oral Health Science, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580, Japan.
authors: Yusuke
authors: Y
authors: Takahashi
authors: Akihiro
authors: A
authors: Yoshida
authors: Masato
authors: M
authors: Nagayoshi
authors: Chiaki
authors: C
authors: Kitamura
authors: Tatsuji
authors: T
authors: Nishihara
authors: Shuji
authors: S
authors: Awano
authors: Toshihiro
authors: T
authors: Ansai
mesh_terms: D001386:Azides / Q000378:metabolism*; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology; D013293:Enterococcus faecalis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005798:Genes, Bacterial / Q000235:genetics; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability; D010485:Periapical Periodontitis / Q000382:microbiology*; D011419:Propidium / Q000031:analogs & derivatives* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001386:Azides; C533957:propidium monoazide; D011419:Propidium