title: Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart.
abstract: Methionine dietary restriction (MetR), like dietary restriction (DR), increases rodent maximum longevity. However, the mechanism responsible for the retardation of aging with MetR is still not entirely known. As DR decreases oxidative damage and mitochondrial free radical production, it is plausible to hypothesize that a decrease in oxidative stress is the mechanism for longevity extension with MetR. In the present investigation male Wistar rats were subjected to isocaloric 40% MetR during 7 weeks. It was found that 40% MetR decreases heart mitochondrial ROS production at complex I during forward electron flow, lowers oxidative damage to mitochondrial DNA and proteins, and decreases the degree of methylation of genomic DNA. No significant changes occurred for mitochondrial oxygen consumption, the amounts of the four respiratory complexes (I to IV), and the mitochondrial protein apoptosis-inducing factor (AIF). These results indicate that methionine can be the dietary factor responsible for the decrease in mitochondrial ROS generation and oxidative stress, and likely for part of the increase in longevity, that takes place during DR. They also highlight some of the mechanisms involved in the generation of these beneficial effects.
authors: Department of Animal Physiology-II, Faculty of Biological Sciences, Complutense University of Madrid (UCM), Madrid, Spain.
authors: Ines
authors: I
authors: Sanchez-Roman
authors: Alexia
authors: A
authors: Gomez
authors: Jose
authors: J
authors: Gomez
authors: Henar
authors: H
authors: Suarez
authors: Carlota
authors: C
authors: Sanchez
authors: Alba
authors: A
authors: Naudi
authors: Victoria
authors: V
authors: Ayala
authors: Manuel
authors: M
authors: Portero-Otin
authors: Monica
authors: M
authors: Lopez-Torres
authors: Reinald
authors: R
authors: Pamplona
authors: Gustavo
authors: G
authors: Barja
mesh_terms: D000818:Animals; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D004249:DNA Damage; D019175:DNA Methylation; D004272:DNA, Mitochondrial / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000378:metabolism*; D008136:Longevity; D008297:Male; D008715:Methionine; D008929:Mitochondria, Heart / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors
chemical_list: D051033:Apoptosis Inducing Factor; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D008715:Methionine; D042967:Electron Transport Complex I