title: Effects of needle-knife therapy on iNOS activity and NO content in skeletal muscles in the L3 transverse process syndrome model rat.
abstract: OBJECTIVE
  To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebrae (L3) transverse process syndrome model rat and to observe the effect of needle-knife therapy.
  
  
  METHODS
  One hundred and sixty male SD rats were randomly divided into normal group, model group, aminoguanidine (AG) group, needle-knife group, 40 rats in each group. The L3 transverse process syndrome rat model was established, and after treatment of needle-knife and AG iNOS activities and NO contents and histomorpholocal changes in the soft tissues around L3 transverse process on 1, 3, 7 and 14 days were observed in the groups.
  
  
  RESULTS
  Compared with the normal group, iNOS activity and NO content in the model group were significantly increased (P < 0.01); Compared with the model group, iNOS activities and NO contents were significantly decreased in both the needle-knife group and the AG group (both P < 0.01); And both iNOS activities and NO contents were identical with both local inflammation response and injured degrees of the injured tissue in the groups.
  
  
  CONCLUSIONS
  Needle-knife therapy can significantly inhibit generation of NO, alleviate inflammatory response and injured degree of the injured soft tissue, improve microcirculation, prevent formation of pathological scar tissue, and promote repair of the chronic soft tissue injury.
authors: Department of Rehabilitation and Physiotherapy, Navy General Hospital, Beijing 100190, China. jinlin195@vip.sohu.com
authors: Jin-Lin
authors: JL
authors: Qiao
authors: Jin-Niu
authors: JN
authors: Li
authors: Guang-Cheng
authors: GC
authors: Ji
authors: Guang-Hao
authors: GH
authors: Ma
authors: Ben-Sheng
authors: BS
authors: Fu
authors: Dong-Dong
authors: DD
authors: Xiang
authors: Yi-Ying
authors: YY
authors: Chen
authors: Ping
authors: P
authors: Lu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D008159:Lumbar Vertebrae; D008297:Male; D008516:Medicine, Chinese Traditional; D018482:Muscle, Skeletal / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013122:Spinal Diseases / Q000378:metabolism / Q000628:therapy*
chemical_list: D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II