title: Role of actin depolymerizing factor in the development of methamphetamine-induced place preference in mice.
abstract: The present study investigated role of actin depolymerizing factor (ADF) in methamphetamine-induced place preference using ADF mutant (ADFm) and wild-type mice (WT). Whereas methamphetamine developed dose-dependently place preference in wild-type mice, methamphetamine-induced place preference was significantly attenuated by ADF mutation. Moreover, the administration of phalloidin, an F-actin stabilizer, dose-dependently inhibited methamphetamine-induced place preference. Methamphetamine-conditioning significantly increased the protein levels of ADF, cofilin, G-actin and F-actin in the limbic forebrain of both WT and ADFm, though the degree of enhancement of G- and F-actins by methamphetamine in ADFm was significantly smaller than in WT. Immunohistochemical study showed the co-localization of synaptophysin and tyrosine hydroxylase in WT and the potentiation of the immunoreactivity of these proteins by methamphetamine-conditioning, whereas such potentiation was suppressed by ADF mutation. In addition, the protein levels of synaptophysin, post-synaptic density 95 (PSD95), and neuroligin1 significantly increased in the limbic forebrain of WT showing methamphetamine-induced place preference, but not in ADFm. These findings indicate that ADF play an important role in the development of methamphetamine-induced place preference via regulation of actin dynamics.
authors: Department of Pharmacology, Kawasaki Medical School, 577 Matsushima, Kurashiki 701-0192, Japan.
authors: Masahiro
authors: M
authors: Shibasaki
authors: Koji
authors: K
authors: Mizuno
authors: Kazuhiro
authors: K
authors: Kurokawa
authors: Tsutomu
authors: T
authors: Suzuki
authors: Seitaro
authors: S
authors: Ohkuma
mesh_terms: D000199:Actins / Q000378:metabolism; D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000378:metabolism; D000818:Animals; D051345:Destrin / Q000235:genetics / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases / Q000378:metabolism; D054461:Lim Kinases / Q000378:metabolism; D008032:Limbic System / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008694:Methamphetamine / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009473:Neuronal Plasticity / Q000187:drug effects; D010590:Phalloidine / Q000494:pharmacology; D013037:Spatial Behavior / Q000187:drug effects* / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000199:Actins; D020410:Activated-Leukocyte Cell Adhesion Molecule; D051345:Destrin; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010590:Phalloidine; D008694:Methamphetamine; D014446:Tyrosine 3-Monooxygenase; D054461:Lim Kinases; C515893:Limk1 protein, mouse; D051528:Guanylate Kinases