title: A Novel Duplex Real-Time Reverse-Transcription PCR Assay for the Detection of Influenza A and the Novel Influenza A(H1N1) Strain.
abstract: Timely implementation of antiviral treatment and other public health based responses are dependent on accurate and rapid diagnosis of the novel pandemic influenza A(H1N1) strain. In this study we developed a duplex real-time PCR (RT-PCR) (dFLU-TM) assay for the simultaneous detection of a broad range of influenza A subtypes and specific detection of the novel H1N1 2009 pandemic strain. The assay was compared to the combined results of two previously described monoplex RT-PCR assays using 183 clinical samples and 10 seasonal influenza A isolates. Overall, the results showed that the dFLU-TM RT-PCR method is suitable for detection of influenza A, including the novel H1N1 pandemic strain, in clinical samples.
authors: Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Queensland Children's Medical Research Institute, Children's Health Service District, Queensland, Australia; E-Mails:  seweryn@uq.edu.au  (S.B.);  d.whiley@uq.edu.au  (D.M.W.);  cassyfaux@uq.edu.au  (C.E.F.);  sblambert@uq.edu.au  (S.B.L.);  theniss@uq.edu.au  (M.D.N.);  t.sloots@uq.edu.au  (T.P.S.).
authors: Rebecca J
authors: RJ
authors: Rockett
authors: Seweryn
authors: S
authors: Bialasiewicz
authors: David M
authors: DM
authors: Whiley
authors: Cheryl
authors: C
authors: Bletchly
authors: Cassandra E
authors: CE
authors: Faux
authors: Stephen B
authors: SB
authors: Lambert
authors: Graeme R
authors: GR
authors: Nimmo
authors: Michael D
authors: MD
authors: Nissen
authors: Theo P
authors: TP
authors: Sloots