title: MicroRNA predictors of longevity in Caenorhabditis elegans.
abstract: Neither genetic nor environmental factors fully account for variability in individual longevity: genetically identical invertebrates in homogenous environments often experience no less variability in lifespan than outbred human populations. Such variability is often assumed to result from stochasticity in damage accumulation over time; however, the identification of early-life gene expression states that predict future longevity would suggest that lifespan is least in part epigenetically determined. Such "biomarkers of aging," genetic or otherwise, nevertheless remain rare. In this work, we sought early-life differences in organismal robustness in unperturbed individuals and examined the utility of microRNAs, known regulators of lifespan, development, and robustness, as aging biomarkers. We quantitatively examined Caenorhabditis elegans reared individually in a novel apparatus and observed throughout their lives. Early-to-mid-adulthood measures of homeostatic ability jointly predict 62% of longevity variability. Though correlated, markers of growth/muscle maintenance and of metabolic by-products ("age pigments") report independently on lifespan, suggesting that graceful aging is not a single process. We further identified three microRNAs in which early-adulthood expression patterns individually predict up to 47% of lifespan differences. Though expression of each increases throughout this time, mir-71 and mir-246 correlate with lifespan, while mir-239 anti-correlates. Two of these three microRNA "biomarkers of aging" act upstream in insulin/IGF-1-like signaling (IIS) and other known longevity pathways, thus we infer that these microRNAs not only report on but also likely determine longevity. Thus, fluctuations in early-life IIS, due to variation in these microRNAs and from other causes, may determine individual lifespan.
authors: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, United States of America.
authors: Zachary
authors: Z
authors: Pincus
authors: Thalyana
authors: T
authors: Smith-Vikos
authors: Frank J
authors: FJ
authors: Slack
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D044127:Epigenesis, Genetic / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D005819:Genetic Markers; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008136:Longevity / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013223:Statistics as Topic
chemical_list: D005819:Genetic Markers; D007328:Insulin; C569803:MIRN239 microRNA, C elegans; C569806:MIRN246 microRNA, C elegans; C575438:MIRN71 microRNA, C elegans; C569804:MIRN74 microRNA, C elegans; D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I