title: Characterization of immune cell infiltration into canine intracranial meningiomas.
abstract: Meningiomas are the most common intracranial tumors in dogs. A variety of inflammatory cells have been shown to invade these tumors in people, but little is known about interactions between the immune system and naturally occurring brain tumors in dogs. The purpose of this study was to investigate the presence of a variety of immune cell subsets within canine intracranial meningiomas. Twenty-three formalin-fixed, paraffin-embedded tumor samples were evaluated using immunohistochemistry with antibodies specific for CD3, CD79a, CD18, CD11d (αD), CD45RA, forkhead box P3, and Toll-like receptors 4 and 9. Immune cell infiltration was evident in all samples, with a predominance of CD3(+) T cells. Large numbers of CD18(+) microglia and macrophages were noted surrounding and infiltrating the tumors, and a subset of these cells within the tumor appeared to be CD11d(+). Scattered macrophages at the tumor-brain interface were TLR4(+) and TLR9(+). Rare CD79a(+) B cells were noted in only a small subset of tumors. Lesser numbers of lymphocytes that were CD11d(+), CD45RA(+), or FoxP3(+) were noted in a number of the meningiomas. Although the function of these cells is not yet clear, work in other species suggests that evaluation of this immune cell infiltrate may provide important prognostic information and may be useful in the design of novel therapies.
authors: Comparative Neuroimmunology and Neurooncology Laboratory, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA.
authors: L B
authors: LB
authors: Boozer
authors: T W
authors: TW
authors: Davis
authors: L B
authors: LB
authors: Borst
authors: K M
authors: KM
authors: Zseltvay
authors: N J
authors: NJ
authors: Olby
authors: C L
authors: CL
authors: Mariani
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004283:Dog Diseases / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D016131:Lymphocyte Subsets / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008577:Meningeal Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008579:Meningioma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D016612:Paraffin Embedding / Q000662:veterinary; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D016207:Cytokines; D051858:Forkhead Transcription Factors; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9