title: Multiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme.
abstract: A two-dimensional (2D) spectral SPR sensor based on a polarization control scheme is reported in this paper. The polarization control configuration converts the phase difference between p- and s- polarization occurring at surface plasmon resonance (SPR) into corresponding color responses in spectral SPR images. A sensor resolution of 2.7 x 10(-6) RIU has been demonstrated, which corresponds to more than one order of magnitude resolution improvement (26 times) comparing to existing 2D spectral SPR sensors. Multiplex array detection has also been demonstrated with the spectral SPR imaging sensor. In a 8 x 4 sensor array, 32 samples with different refractive index values were monitored simultaneously. Detection on bovine serum albumin (BSA) antigen-antibody binding further demonstrated the multiplex detection capability of the 2D spectral SPR sensor for bio-molecular interactions. The detection limit is found to be 21 ng/ml, which is 36 times better than the detection limit previously reported by phase imaging SPR sensors. In light of the advantages of high sensitivity, 2D multiplex detection and real-time response, the spectral SPR imaging sensor can find promising applications in rapid, high throughput, non-labeling and multiplex detection of protein array for proteomics studies, biomarker screening, disease prognosis, and drug discovery.
authors: Division of Microelectronics, School of Electrical & Electronic Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 639798, Singapore. wongchilok@ntu.edu.sg
authors: Chi Lok
authors: CL
authors: Wong
authors: George Chung Kit
authors: GC
authors: Chen
authors: Beng Koon
authors: BK
authors: Ng
authors: Shuchi
authors: S
authors: Agarwal
authors: Zhiping
authors: Z
authors: Lin
authors: Peng
authors: P
authors: Chen
authors: Ho Pui
authors: HP
authors: Ho
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D004867:Equipment Design; D008027:Light; D057230:Limit of Detection; D040081:Protein Array Analysis / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D012710:Serum Albumin, Bovine