title: Identification of alkaline stress-responsive genes of CBL family in sweet sorghum (Sorghum bicolor L.).
abstract: Calcineurin B-like proteins play important roles in the calcium perception and signal transduction of abiotic stress. In this study, the bioinformatic analysis of molecular characteristics of Sorghum bicolor calcineurin B-like protein (SbCBL) revealed that sequences of SbCBL are highly conserved, and most SbCBLs have three typical EF-hands structures. Among the SbCBL proteins, four of which, SbCBL01, 04, 05, 08, have a conserved N-myristoylation domain. Stress-responsive and phytohormone-responsive cis-elements were found in the promoter regions of SbCBL genes. Real-time quantitative polymerase chain reaction (RTqPCR) analysis showed that SbCBL genes have different tissue-specific expression patterns under normal growth conditions in sweet sorghum (Sorghum bicolor L. Moench). Interestingly, when treated with sodium carbonate, SbCBL genes also show various sodium carbonate stress responsive patterns in sweet sorghum seedlings. These results suggest that SbCBLs may participate in regulating sodium carbonate stress-specific cellular adaptation responses and influencing growth and developmental patterns in sweet sorghum.
authors: Laboratory of Soil and Plant Molecular Genetics, College of Plant Science, Jilin University, Changchun 130062, China.
authors: Chunxia
authors: C
authors: Zhang
authors: Mingdi
authors: M
authors: Bian
authors: Hui
authors: H
authors: Yu
authors: Qing
authors: Q
authors: Liu
authors: Zhenming
authors: Z
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D002254:Carbonates / Q000494:pharmacology*; D032461:Chromosomes, Plant / Q000235:genetics; D019295:Computational Biology; D018548:Cotyledon / Q000187:drug effects / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018745:Genome, Plant / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D016415:Sequence Alignment; D015398:Signal Transduction / Q000187:drug effects; D045868:Sorghum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D002254:Carbonates; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C005686:sodium carbonate