title: Evaluation of third-generation RIDASCREEN enzyme immunoassay for the detection of norovirus antigens in stool samples of hospitalized children in Belém, Pará, Brazil.
abstract: Noroviruses (NoVs) are major agents of gastroenteritis outbreaks and hospitalization worldwide. This study evaluated the sensitivity and specificity of the commercially available third-generation RIDASCREEN® Norovirus Enzyme Immunoassay (EIA) kit in comparison to the reverse transcription-polymerase chain reaction (RT-PCR) to detect NoVs in hospitalized children with gastroenteritis. An agreement of 88% (81/92) was observed when comparing EIA with RT-PCR. A sensitivity of 92% and a specificity of 83.3% were demonstrated. Eleven samples were positive by 1 method only (4 RT-PCR/7 EIA). Fourteen samples were sequenced and all classified as NoV genogroup GII-4. The 7 positive only by EIA were also evaluated by electron microscopy, and in 3 (42.9%) samples viral particles with a suggestive morphology of NoVs were visualized. These same samples were tested by seminested-RT-PCR with a positivity of 85.7%. The results obtained in this study demonstrated a significant improvement in the sensitivity and specificity of this updated assay.
authors: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, Pará, Brasil.
authors: Jones Anderson Monteiro
authors: JA
authors: Siqueira
authors: Alexandre da Costa
authors: Ada C
authors: Linhares
authors: Darleise de Souza
authors: Dde S
authors: Oliveira
authors: Luana da Silva
authors: Lda S
authors: Soares
authors: Maria Silvia Sousa
authors: MS
authors: Lucena
authors: Ana Lúcia Monteiro
authors: AL
authors: Wanzeller
authors: Joana D'Arc Pereira
authors: JD
authors: Mascarenhas
authors: Yvone Benchimol
authors: YB
authors: Gabbay
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D001938:Brazil; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D002673:Child, Hospitalized; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D046529:Microscopy, Electron, Transmission; D029322:Norovirus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014771:Virion / Q000648:ultrastructure
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral