title: Cryosurgery and rhTNF-α play synergistic effects on a rat cortex C6 glioma model.
abstract: Glioma, a type of brain tumor originating from glioma cells, varies widely in aggressiveness and causes serious symptoms, but the treatments are limited. Studies have shown that cryosurgery has multiple effects on tumor treatments, and administration of human tumor necrosis factor-alpha (rhTNF-α) arguments the anti-tumor effect of cryotherapy in breast and prostate cancers. To test the hypothesis that cryosurgery and rhTNF-α play synergistic effects against brain tumors, we established a brain glioma model on rat cortex regions following different treatments: the G1 group was sham-operated; the G2 group was treated with cryosurgery; the G3 group was treated with rhTNF-α; and G4 group received combined treatment with cryosurgery and rhTNF-α. Tumor sizes were measured by magnetic resonance imaging; DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL assay); P21(WAF1/CIP1) and proliferating cell nuclear antigen (PCNA) expression levels were scored using immunohistochemical staining. G2 and G4 rats had significantly longer survival time than did G1 rats. Tumor sizes in each group were significantly decreased as compared with those in G1 rats. PCNA-positive cells were significantly decreased in G2, G3 and G4 rats as compared with G1 rats. In contrast, DNA fragmentation and P21(WAF1/CIP1)-positive cells were significantly increased in each treatment group. Importantly, a combined treatment enhanced the effects of cryosurgery. Combined treatment with cryosurgery and rhTNF-α may have a synergistic effect on glioma tumor therapy, enhancing the inhibition of proliferation and the induction of apoptosis.
authors: Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, China. hkm1111@sina.com
authors: Kuan-Ming
authors: KM
authors: Huang
authors: Min
authors: M
authors: Peng
authors: Yan-Qin
authors: YQ
authors: Feng
authors: Hui
authors: H
authors: Huang
authors: Han-Jun
authors: HJ
authors: Tu
authors: Jie
authors: J
authors: Luo
authors: Li
authors: L
authors: Zhang
authors: Xian-Hou
authors: XH
authors: Yuan
authors: Lun-Chang
authors: LC
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D002540:Cerebral Cortex / Q000473:pathology; D003131:Combined Modality Therapy; D003452:Cryosurgery; D053938:DNA Fragmentation; D005910:Glioma / Q000188:drug therapy / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D020287:In Situ Nick-End Labeling; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha