title: Studies on conjugation of Spirogyra using monoclonal culture.
abstract: We succeeded in inducing conjugation of Spirogyra                         castanacea by incubating algal filaments on agar plate. Conjugation could be induced using clone culture. The scalariform conjugation was generally observed, while lateral conjugation was rarely. When two filaments formed scalariform conjugation, all cells of one filament behaved as male and those of other filament did as female. Very rarely, however, zygospores were formed in both of pair filaments. The surface of conjugation tube was stained with fluorescently labeled-lectins, such as Bandeiraea (Griffonia) simplicifolia lectin (BSL-I) and jacalin. BSL-I strongly stained the conjugation tubes, while weakly did the cell surface of female gamete first and then that of male gamete. Jacalin stained mainly the conjugation tubes. Addition of jacalin inhibited the formation of papilla, suggesting some important role of jacalin-binding material at the initial step of formation of the conjugation tubes.
authors: Pioneering Research Unit for Next Generation, Kyoto University, Uji, Kyoto 611-0011, Japan. h-ikegaya@iss.iae.kyoto-u.ac.jp
authors: Hisato
authors: H
authors: Ikegaya
authors: Takuto
authors: T
authors: Nakase
authors: Kazuyoshi
authors: K
authors: Iwata
authors: Hideaki
authors: H
authors: Tsuchida
authors: Seiji
authors: S
authors: Sonobe
authors: Teruo
authors: T
authors: Shimmen
mesh_terms: D059001:Aquatic Organisms / Q000502:physiology; D055993:Germ Cells, Plant / Q000166:cytology* / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology*; D058131:Spirogyra / Q000166:cytology* / Q000254:growth & development*
chemical_list: D010937:Plant Growth Regulators; D037121:Plant Lectins; C043696:jacalin