title: Bioactivity of immobilized hyaluronic acid derivatives regarding protein adsorption and cell adhesion.
abstract: Hyaluronic acid (HA) was chemically modified either by oxidation to obtain aldehyde-HA (aHA) or 3,3'-dithiobis(propanoic hydrazide) to obtain thiol-HA (tHA) that was covalently immobilized on model substrata such as amino-terminated surfaces or gold. Knowledge about the effect of modification with HA on physicochemical surface properties of these substrata and estimates of the quantities of immobilized HA were obtained by different physical methods such as contact angle measurements, ellipsometry, and atomic force microscopy. The bioactivity of aHA and tHA toward their natural binding partner aggrecan was studied by comparing surface plasmon resonance to native HA; this shows that binding of aggrecan was achieved in a similar way. Dermal human fibroblasts were used as a model cell to study how chemical modification and immobilization of HA impact adhesion and spreading of cells, which also affects cell growth and differentiation. A lower number and spreading of cells were observed on HA-modified surfaces compared to amino- and vinyl-terminated glass and silicon surfaces. Immunofluorescence microscopy also revealed that adhesion of fibroblast plated on HA-modified surfaces was mediated primarily by HA receptor CD44, indicating that bioactivity of HA was not significantly reduced by chemical modification.
authors: Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
authors: Alexander
authors: A
authors: Köwitsch
authors: Yuan
authors: Y
authors: Yang
authors: Ning
authors: N
authors: Ma
authors: Judith
authors: J
authors: Kuntsche
authors: Karsten
authors: K
authors: Mäder
authors: Thomas
authors: T
authors: Groth
mesh_terms: D000327:Adsorption; D053674:Aggrecans / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D010084:Oxidation-Reduction; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D053674:Aggrecans; D013438:Sulfhydryl Compounds; D006820:Hyaluronic Acid