title: [Influence of mannose binding lectin to expression of TGF-beta1 and NF-kappaB in high concentration of glucose cultured human renal glomerular endothelial cells].
abstract: OBJECTIVE
  To investigate the effect of mannose binding lectin (MBL) complement pathway on expression of transforming growth factor-beta1 (TGF-beta1) and NF-kappaB in cultured human renal glomerular endothelial cells (HRGECs) stimulated by high concentration of glucose.
  
  
  METHODS
  Human glomerular endothelial cells in culture were randomly divided into 5 groups according to different managements: normal concentration of glucose as controlled group, MBL + normal concentration of glucose group, high concentration of glucose, MBL + high glucose and MBL + high glucose + MBL blocker respectively. Flow cytometry was used to detect the depositions of MBL and C3 on the surfaces of HRGECs. Real-time PCR method was used to detect the mRNA levels of TGF-beta1. Human TGF-beta1 ELISA kit was used to detect the concentration of TGF-beta1 in supernatant fluid. ESMA was used to detect the activity of NF-kappaB in HRGECs.
  
  
  RESULTS
  Compared with the normal glucose group and high glucose group, the depositions of MBL, C3 were apparently increased in MBL + high glucose group (P < 0.05). Expression of TGF-beta1 were significantly higher (P < 0.05) in MBL + high concentration of glucose groups than the normal glucose group and the high concentration of glucose group. Compared with the high glucose group, the activity of NF-kappaB in HRGECs was apparently increased in MBL + high glucose group, which could be significantly downregulated by MBL blocking antibody.
  
  
  CONCLUSIONS
  High concentration of glucose can increase the expression of TGF-beta1 of cultured human glomerular endothelial cells. At the same time, high glucose together with MBL can up regulate the expression of TGF-beta1 and the activity of NF-kappaB in HRGECs.
authors: Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Hong-Yu
authors: HY
authors: Qiu
authors: Wen-Xing
authors: WX
authors: Fan
authors: Chuan
authors: C
authors: Zuo
authors: Song-Min
authors: SM
authors: Huang
authors: Fang
authors: F
authors: Liu
authors: Wan-Xin
authors: WX
authors: Tang
mesh_terms: D002478:Cells, Cultured; D037582:Complement Pathway, Mannose-Binding Lectin / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D037601:Mannose-Binding Lectin; D016328:NF-kappa B; D053773:Transforming Growth Factor beta1; D005947:Glucose