title: Global analysis of the genes involved in the thermotolerance mechanism of thermotolerant Acetobacter tropicalis SKU1100.
abstract: Acetobacter tropicalis SKU1100 is a thermotolerant acetic acid bacterium that grows even at 42 °C, a much higher temperature than the limit for the growth of mesophilic strains. To elucidate the mechanism underlying the thermotolerance of this strain, we attempted to identify the genes essential for growth at high temperature by transposon (Tn10) mutagenesis followed by gene or genome analysis. Among the 4,000 Tn10-inserted mutants obtained, 32 exhibited a growth phenotype comparable to that of the parent strain at 30 °C but not at higher temperatures. We identified the insertion site of Tn10 on the chromosomes of all the mutant strains by TAIL (Thermal Asymmetric Interlaced)-PCR, and found 24 genes responsible for thermotolerance. The results also revealed a partial overlap between the genes required for thermotolerance and those required for acetic acid resistance. In addition, the origin and role of these thermotolerant genes are discussed.
authors: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors: Wichai
authors: W
authors: Soemphol
authors: Arpaporn
authors: A
authors: Deeraksa
authors: Minenosuke
authors: M
authors: Matsutani
authors: Toshiharu
authors: T
authors: Yakushi
authors: Hirohide
authors: H
authors: Toyama
authors: Osao
authors: O
authors: Adachi
authors: Mamoru
authors: M
authors: Yamada
authors: Kazunobu
authors: K
authors: Matsushita
mesh_terms: D000091:Acetobacter / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D000220:Adaptation, Biological / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D023281:Genomics; D009154:Mutation; D017421:Sequence Analysis; D013696:Temperature
chemical_list: D004251:DNA Transposable Elements