title: Genital and extra-genital screening for gonorrhoea using the BD Probetec ET system with an in-house PCR method targeting the porA pseudogene as confirmatory test.
abstract: Diagnosing gonorrhoea from extra-genital as well as genital sites is important in managing this sexually transmitted disease. In this study we evaluated a screening procedure for Neisseria gonorrhoeae (GC) from all sample sites in a low-prevalence setting. A total of 69,252 specimens submitted for Chlamydia trachomatis testing were also examined for GC on the BD Viper™ platform using the BD Probetec ET system. In order to avoid false-positive results all GC BD reactive samples were re-tested using a PCR method with the porA pseudogene as target. Using this method we screened 170% more samples for GC than in the previous year, in the same population, and diagnosed more than twice as many GC-positive episodes. The BD system can be used successfully to screen extra-genital as well as genital specimen types for GC in a low-prevalence area if it is combined with a validated confirmatory PCR test.
authors: Department of Clinical Microbiology, Hvidovre Hospital, Denmark.
authors: Sissel
authors: S
authors: Skovgaard
authors: Helle Kiellberg
authors: HK
authors: Larsen
authors: Carsten
authors: C
authors: Sand
authors: Alice
authors: A
authors: Friis-Møller
authors: Kristian
authors: K
authors: Schønning
authors: Jørgen Skov
authors: JS
authors: Jensen
authors: Henrik
authors: H
authors: Westh
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology*; D003718:Denmark; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques; D010614:Pharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D018272:Porins / Q000235:genetics; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012007:Rectum / Q000382:microbiology*; D014521:Urethra / Q000382:microbiology*
chemical_list: D018272:Porins; D011933:Reagent Kits, Diagnostic; C057699:porin protein, Neisseria; D036781:Fimbriae Proteins