title: Sustained Fos expression is observed in the developing brainstem auditory circuits of kanamycin-treated rats.
abstract: It has been demonstrated that kanamycin treatment during early developmental period induces partial cochlear destruction and enhanced glutamatergic transmission at the medial nucleus of the trapezoid body (MNTB) - the lateral superior olive (LSO) synapses in the superior olivary complex (SOC). As c-fos was expected to be expressed in the SOC by kanamycin-induced cochlear damage, the expression of c-fos protein (Fos) was investigated using immunohistochemistry in kanamycin-treated rat pups. In the control rat pups less than postnatal (P) day 9 in age, Fos-like immunoreactivity (Fos-IR) was transiently observed in the MNTB and LSO on P6, but disappeared on P9, which reflects a physiologic process. In contrast, in kanamycin-treated rats, Fos-IR was consistently observed through P9. Because a significant increase in terminal uridine deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick-end labeling (TUNEL) and glial fibrillary acidic protein (GFAP) IR was not demonstrated in the MNTB and LSO of kanamycin-treated rats, the increased Fos-IR does not appear to indicate an ongoing pathologic process, but may be related to the increased activity caused by the disturbance in excitatory and inhibitory balance between brainstem auditory circuits.
authors: Institute of Tissue Regeneration Engineering, Dankook University, San 29, Anseo-dong, Cheonan-si, Chungnam 330-714, Republic of Korea.
authors: Jae Ho
authors: JH
authors: Lee
authors: Hee Jung
authors: HJ
authors: Kim
authors: Myung-Whan
authors: MW
authors: Suh
authors: Seung Cheol
authors: SC
authors: Ahn
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001306:Auditory Pathways / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001933:Brain Stem / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007612:Kanamycin / Q000633:toxicity*; D011247:Pregnancy; D011500:Protein Synthesis Inhibitors / Q000633:toxicity*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats
chemical_list: D011500:Protein Synthesis Inhibitors; D016760:Proto-Oncogene Proteins c-fos; D007612:Kanamycin