title: Method enabling gene expression studies of pathogens in a complex food matrix.
abstract: We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes). Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.
authors: Department of Veterinary Disease Biology, University of Copenhagen, Stigboejlen 4, DK-1870 Frederiksberg, Denmark.
authors: Jette
authors: J
authors: Kjeldgaard
authors: Sidsel
authors: S
authors: Henriksen
authors: Marianne Thorup
authors: MT
authors: Cohn
authors: Søren
authors: S
authors: Aabo
authors: Hanne
authors: H
authors: Ingmer
mesh_terms: D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D005511:Food Handling / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D006358:Hot Temperature; D008460:Meat / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D012492:Salts / Q000633:toxicity
chemical_list: D012329:RNA, Bacterial; D012492:Salts