title: Acetylation of Prrp K150 regulates the subcellular localization.
abstract: Posttranslational modifications of proteins have profound effects on many aspects of their function and have received much attention due to the importance of these processes in epigenetic regulation. In this study, we report that deleted azoospermia associated protein 1 (DAZAP1)/proline-rich RNA binding protein (Prrp), a multifunctional RNA binding protein which is essential for spermatogenesis and normal cell growth, is acetylated at Lysine 150 within its RNA binding domain. The acetylation is predominantly observed in nuclear Prrp, and the nonacetylated form is in cytoplasm. Considering that Prrp is a shuttling protein, we suggest that the acetylation cycle at Prrp K150 regulates nucleocytoplasmic transport in cells.
authors: Graduate School of Environment and Information Science, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan.
authors: Kenta
authors: K
authors: Sasaki
authors: Atsushi
authors: A
authors: Suzuki
authors: Satoshi
authors: S
authors: Kagatsume
authors: Mana
authors: M
authors: Ono
authors: Katsuhiko
authors: K
authors: Matsuzawa
authors: Yusuke
authors: Y
authors: Taguchi
authors: Yasuyuki
authors: Y
authors: Kurihara
mesh_terms: D000107:Acetylation; D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: C412481:DAZAP1 protein, human; D016601:RNA-Binding Proteins; D003609:Dactinomycin