title: An antihypertensive peptide from tilapia gelatin diminishes free radical formation in murine microglial cells.
abstract: A peptide possessing antihypertensive activity was purified from Nile tilapia ( Oreochromis niloticus ) gelatin using alcalase, Pronase E, pepsin, and trypsin. Among them, the alcalase hydrolysate exhibited the highest angiotensin converting enzyme (ACE) inhibitory activity. Therefore, it was further analyzed, and a potent ACE inhibitory peptide of DPALATEPDPMPF (1382 Da) was separated and purified. In addition, the protective effect of the purified peptide against free radical-induced cellular and DNA damage in murine microglial cells (BV-2) was determined. These results suggest that the peptide isolated from Nile tilapia (O. niloticus) gelatin acts as a candidate against hypertension and oxidative stress and could be used in health-functional foods.
authors: Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
authors: Thanh-Sang
authors: TS
authors: Vo
authors: Dai-Hung
authors: DH
authors: Ngo
authors: Jung-Ae
authors: JA
authors: Kim
authors: Bomi
authors: B
authors: Ryu
authors: Se-Kwon
authors: SK
authors: Kim
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D029941:Fish Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D006973:Hypertension / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D017210:Tilapia / Q000378:metabolism*
chemical_list: D000959:Antihypertensive Agents; D029941:Fish Proteins; D005609:Free Radicals; D010455:Peptides; D005780:Gelatin