title: Biological control of  Botrytis cinerea  using the antagonistic and endophytic  Burkholderia cepacia  Cs5 for vine plantlet protection.
abstract: Antifungal activity of the Burkholderia cepacia Cs5 was tested in vitro and in vivo for the control of Botrytis cinerea . Bacterial biomass was significantly improved by the amendment of ZnSO(4), Mo(7)(NH(4))(6)O(24), and mannitol to the NBY medium; consequently, the amount of the secreted fungicides was increased. The quantification of B. cinerea inhibition, in liquid and solid conditions, showed an important sensitivity of this fungus to the strain Cs5 fungicides. Microscopic monitoring impact of these fungicides on mycelium structure showed an important increase in their diameter and ramifications in the presence of 0.75% supernatant. For the in vivo application of the strain Cs5, Vitis vinifera plantlets were inoculated with a Cs5 bacterial suspension, then with B. cinerea spores. The plantlets protection was total and durable when these two inoculations were made 3 weeks apart, which is the time for the endophytic bacterium to colonize the plantlets up to the top leaves. This protection is due to Cs5 antagonism and the elicitation of the plantlets self-defense via the root overgrowth.
authors: Equipe « Biopesticides » L.P.A.P., Centre de Biotechnologie de Sfax, Université de Sfax, Tunisie.
authors: Olfa
authors: O
authors: Kilani-Feki
authors: Samir
authors: S
authors: Jaoua
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D061046:Biological Control Agents; D020171:Botrytis / Q000187:drug effects / Q000502:physiology*; D016956:Burkholderia cepacia / Q000737:chemistry / Q000502:physiology*; D010935:Plant Diseases / Q000517:prevention & control; D018515:Plant Leaves / Q000254:growth & development / Q000382:microbiology; D018517:Plant Roots / Q000254:growth & development; D027843:Vitis / Q000254:growth & development / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D061046:Biological Control Agents