title: Vascular targeted single-walled carbon nanotubes for near-infrared light therapy of cancer.
abstract: A new approach for targeting carbon nanotubes to the tumor vasculature was tested using human endothelial cells and MCF-7 breast cancer cells in vitro. Single-walled carbon nanotubes were functionalized with the F3 peptide using a polyethylene glycol linker to target nucleolin, a protein found on the surface of endothelial cells in the vasculature of solid tumors. Confocal microscopy and Raman analysis confirmed that the conjugate was internalized by actively dividing endothelial cells. Dividing endothelial cells were used to mimic these cells in the tumor vasculature. Incubation with the conjugate for 8 h or more caused significant cell death in both actively dividing endothelial cells and MCF-7 breast cancer cells, an effect that is hypothesized to be due to the massive uptake of the conjugate. This targeted cell killing was further enhanced when coupled with near-infrared laser treatment. For confluent (non-dividing) endothelial cells, no cytotoxic effect was seen for incubation alone or incubation coupled with laser treatment. These results are promising and warrant further studies using this conjugate for cancer treatment in vivo.
authors: School of Chemical, Biological and Materials Engineering, University of Oklahoma, 100 East Boyd, Room T-335, Norman, OK 73019, USA.
authors: Whitney M
authors: WM
authors: Prickett
authors: Brent D
authors: BD
authors: Van Rite
authors: Daniel E
authors: DE
authors: Resasco
authors: Roger G
authors: RG
authors: Harrison
mesh_terms: D000704:Analysis of Variance; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007259:Infrared Rays; D018613:Microscopy, Confocal; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D010778:Photochemotherapy / Q000379:methods; D010789:Phototherapy / Q000295:instrumentation*; D011092:Polyethylene Glycols; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D000097796:Nucleolin
chemical_list: D004337:Drug Carriers; D037742:Nanotubes, Carbon; D010455:Peptides; D010750:Phosphoproteins; D011092:Polyethylene Glycols; D016601:RNA-Binding Proteins; D000097796:Nucleolin