title: Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function.
abstract: Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
authors: Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, USA.
authors: Shengli
authors: S
authors: Zhao
authors: Jonathan T
authors: JT
authors: Ting
authors: Hisham E
authors: HE
authors: Atallah
authors: Li
authors: L
authors: Qiu
authors: Jie
authors: J
authors: Tan
authors: Bernd
authors: B
authors: Gloss
authors: George J
authors: GJ
authors: Augustine
authors: Karl
authors: K
authors: Deisseroth
authors: Minmin
authors: M
authors: Luo
authors: Ann M
authors: AM
authors: Graybiel
authors: Guoping
authors: G
authors: Feng
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D000075402:Channelrhodopsins; D002795:Choline O-Acetyltransferase / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009417:Nerve Tissue / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D014365:Tryptophan Hydroxylase / Q000235:genetics; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000235:genetics
chemical_list: D000075402:Channelrhodopsins; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; C487802:Tph2 protein, mouse; D014365:Tryptophan Hydroxylase; D002795:Choline O-Acetyltransferase