title: Effect of glucocorticoid in mice of asthma induced by ovalbumin sensitisation and RSV infection.
abstract: OBJECTIVE
  To investigate the inflammatory changes and the airway hyper-responsiveness in the asthma mouse model infected by respiratory syncytial virus and elucidate the relationship between the infection and the effect of glucocorticoid.
  
  
  METHODS
  60 BALB/c mice were randomly divided into 6 groups. One of these is the control group; the others are the OVA/sham group, the OVA/sham +Dex group, the PBS/RSV group, the OVA/RSV group and the OVA/RSV+Dex group. The airway resistance was measured using a sealed body plethysmograph. Pathological slides were stained with hematoxylin-eosin, and the peribronchial inflammation was observed microscopically. The concentrations of IL-4, IFN-gamma, TGF-beta1 in lung tissues were detected by ELISA.
  
  
  RESULTS
  Compared with the control group, the degree of the airway inflammation and hyperresponsiveness and the concentrations of IL-4/IFN-gamma, TGF-beta1 in all four OVA groups increased significantly. And there was a statistically significant difference between the OVA/sham group and the OVA/sham+Dex group, and between the OVA/RSV group and the OVA/RSV+Dex group respectively. Compared with the OVA/RSV group, there was an obvious aggravation of airway inflammation and hyper-responsiveness in the OVA/RSV+Dex group.
  
  
  CONCLUSIONS
  Glucocorticoid significantly reduces airway inflammation and hyper-responsiveness induced by repetitive OVA challenge in the mouse model of asthma. However, the significant decrease in Th1 and increase in Th2 inflammation and aggravation of airway hyper-responsiveness in the mice in OVA/RSV group show that they are not sensitive to glucocorticoid. The effects of infection with RSV on the mouse model of asthma could be the cause of the glucocorticoid resistance during the therapy.
authors: Department of Healthcare, Provincial Hospital Affiliated to Shandong University, 324#, Jingwu Road, Jinan, 250021, PR China. chixiangyu7216@yahoo.com.cn
authors: Xiang-yu
authors: XY
authors: Chi
authors: Shu-juan
authors: SJ
authors: Jiang
authors: Jing
authors: J
authors: Wang
authors: Jian-ping
authors: JP
authors: Wang
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D016535:Bronchial Hyperreactivity; D002460:Cell Line; D003907:Dexamethasone / Q000008:administration & dosage* / Q000009:adverse effects; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010991:Plethysmography; D018357:Respiratory Syncytial Virus Infections / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D012136:Respiratory Syncytial Viruses / Q000472:pathogenicity / Q000502:physiology*; D058408:Th1-Th2 Balance / Q000187:drug effects
chemical_list: D000485:Allergens; D003907:Dexamethasone; D010047:Ovalbumin