title: Calcium imaging in the optical stretcher.
abstract: The Microfluidic Optical Stretcher (MOS) has previously been shown to be a versatile tool to measure mechanical properties of single suspended cells. In this study we combine optical stretching and fluorescent calcium imaging. A cell line transfected with a heat sensitive cation channel was used as a model system to show the versatility of the setup. The cells were loaded with the Ca(2+) dye Fluo-4 and imaged with confocal laser scanning microscopy while being stretched. During optical stretching heat is transferred to the cell causing a pronounced Ca(2+) influx through the cation channel. The technique opens new perspectives for investigating the role of Ca(2+) in regulating cell mechanical behavior.
authors: Institut für Experimentelle Physik I, Abteilung für Physik der Weichen Materie, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany. Markus.Gyger@uni-leipzig.de
authors: Markus
authors: M
authors: Gyger
authors: Daniel
authors: D
authors: Rose
authors: Roland
authors: R
authors: Stange
authors: Tobias
authors: T
authors: Kiessling
authors: Mareike
authors: M
authors: Zink
authors: Ben
authors: B
authors: Fabry
authors: Josef A
authors: JA
authors: Käs
mesh_terms: D002118:Calcium / Q000032:analysis*; D002478:Cells, Cultured; D006801:Humans; D007668:Kidney / Q000737:chemistry* / Q000166:cytology; D018613:Microscopy, Confocal / Q000295:instrumentation*
chemical_list: D002118:Calcium