title: Antiproliferative activity of N(6)-isopentenyladenosine on HCT-15 colon carcinoma cell line.
abstract: N(6)-Isopentenyladenosine (iPA), a member of the cytokinin family of plant hormones, exerts remarkable inhibition on tumor cell proliferation and apoptosis in several tumor cell lines. In this study, we report that iPA is able to inhibit the proliferation and promotes apoptosis in HCT-15 human colon cancer cells in a dose-dependent manner with a concentration of 2.5 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. Moreover, suppression of clonogenic activity occurs after exposure to iPA at a concentration of 2.5 μM for HCT-15.
authors: Department of Chemistry and Biochemistry, Islamic Azad University , Neyshabur Branch, Neyshabur, Iran. m.rajabi.s@gmail.com
authors: Mehdi
authors: M
authors: Rajabi
authors: Jamshid
authors: J
authors: Mehrzad
authors: Elena
authors: E
authors: Gorincioi
authors: Enzo
authors: E
authors: Santaniello
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D003110:Colonic Neoplasms; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007541:Isopentenyladenosine / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D007541:Isopentenyladenosine