title: IL-6 regulates exercise and training-induced adaptations in subcutaneous adipose tissue in mice.
abstract: OBJECTIVE
  The aim of this study was to test the hypothesis that IL-6 regulates exercise-induced gene responses in subcutaneous adipose tissue in mice.
  
  
  METHODS
  Four-month-old male IL-6 whole body knockout (KO) mice and C57B wild-type (WT) mice performed 1 h of treadmill exercise, where subcutaneous adipose tissue (AT) was removed either immediately after, 4 h or 10 h after exercise as well as from mice not running acutely. Moreover, AT was sampled at resting conditions after 5 weeks of exercise training.
  
  
  RESULTS
  AT leptin mRNA decreased immediately after a single running exercise bout in both genotypes and returned to baseline within 10 h of recovery in IL-6 KO mice, but not WT mice. Leptin mRNA content decreased in WT and increased in IL-6 KO mice with training, but without significant alterations in leptin protein. Acute exercise induced a decrease in the AT TNFα mRNA content in WT, but not in IL-6-KO mice, while training lowered resting levels of TNFα mRNA in both genotypes. In addition, an exercise-induced decline in AT PPARγ mRNA content was absent in IL-6 KO mice and in line training increased PPARγ mRNA only in IL-6 KO mice.
  
  
  CONCLUSIONS
  The present findings indicate a role of IL-6 in regulating exercise- and training-induced leptin and PPARγ expression in adipose tissue. In addition, while IL-6 is required for TNF-α mRNA reduction in response to acute exercise, IL-6 does not appear to be mandatory for anti-inflammatory effects of exercise training in adipose tissue.
authors: Centre of Inflammation and Metabolism, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark. Cbrandt@inflammationmetabolism.dk
authors: C
authors: C
authors: Brandt
authors: A H
authors: AH
authors: Jakobsen
authors: H
authors: H
authors: Adser
authors: J
authors: J
authors: Olesen
authors: N
authors: N
authors: Iversen
authors: J M
authors: JM
authors: Kristensen
authors: P
authors: P
authors: Hojman
authors: J F P
authors: JF
authors: Wojtaszewski
authors: J
authors: J
authors: Hidalgo
authors: H
authors: H
authors: Pilegaard
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010805:Physical Conditioning, Animal / Q000502:physiology*; D050151:Subcutaneous Fat / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha