title: Purification and partial characterization of low molecular weight vicilin-like glycoprotein from the seeds of Citrullus lanatus.
abstract: The watermelon (Citrullus lanatus) seeds are highly nutritive and contain large amount of proteins and many beneficial minerals such as magnesium, calcium, potassium, iron, phosphorous, zinc etc. In various parts of the world, C. lanatus seed extracts are used to cure cancer, cardiovascular diseases, hypertension, and blood pressure. C. lanatus seed extracts are also used as home remedy for edema and urinary tract problems. In this study, we isolated protein fraction of C. lanatus seeds using various protein separation methods. We successfully purified a low molecular weight vicilin-like glycoprotein using chromatographic methods followed by SDS-PAGE and MALDI-TOF/MS identification. This is the first report of purification of a vicilin like polypeptide from C. lanatus seeds. In next step, we extracted mRNA from immature seeds and reverse transcribed it using suitable forward and reverse primers for purified glycoprotein. The PCR product was analysed on 1% agarose gel and was subsequently sequenced by Dideoxy DNA sequencing method. An amino acid translation of the gene is in agreement with amino acid sequences of the identified peptides.
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors: Sushila
authors: S
authors: Yadav
authors: Anil Kumar
authors: AK
authors: Tomar
authors: O
authors: O
authors: Jithesh
authors: Meraj Alam
authors: MA
authors: Khan
authors: R N
authors: RN
authors: Yadav
authors: A
authors: A
authors: Srinivasan
authors: Tej P
authors: TP
authors: Singh
authors: Savita
authors: S
authors: Yadav
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029773:Citrullus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D055314:Seed Storage Proteins; C015575:vicilin protein, plant