title: MutL associates with Escherichia coli RecA and inhibits its ATPase activity.
abstract: Different DNA repair systems are known to cooperate to deal with DNA damage. However, the regulatory role of the cross-talk between these pathways is unclear. Here, we have shown that MutL, an essential component of mismatch repair, is a RecA-interacting protein, and that its highly conserved N-terminal domain is sufficient for this interaction. Surface plasmon resonance and capillary electrophoresis analyses revealed that MutL has little effect on RecA-ssDNA filament formation, but dose down-regulate the ATPase activity of RecA. Our findings identify a new role for MutL, and suggest its regulatory role in homologous recombination.
authors: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors: Min
authors: M
authors: Zhang
authors: Ying
authors: Y
authors: Zhou
authors: Tao
authors: T
authors: Li
authors: Hailin
authors: H
authors: Wang
authors: Fang
authors: F
authors: Cheng
authors: Yafeng
authors: Y
authors: Zhou
authors: Lijun
authors: L
authors: Bi
authors: Xian-En
authors: XE
authors: Zhang
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D053843:DNA Mismatch Repair; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D000070956:MutL Proteins; D054730:Protein Interaction Domains and Motifs; D011938:Rec A Recombinases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; C074770:MutL protein, E coli; D011938:Rec A Recombinases; D000251:Adenosine Triphosphatases; D000070956:MutL Proteins