title: Novel probiotic Bifidobacterium longum subsp. infantis CECT 7210 strain active against rotavirus infections.
abstract: Rotavirus is the leading cause of severe acute gastroenteritis among children worldwide. It is well known that breast-feeding and vaccination afford infants protection. Since breast-feeding has drastically decreased in developed countries, efforts have been focused on the potential use of probiotics as preventive agents. In this study, a novel Bifidobacterium longum subsp. infantis strain was isolated from infant feces and selected, based on its capacity to inhibit in vitro rotavirus Wa replication (up to 36.05% infectious foci reduction) and also to protect cells from virus infection (up to 48.50% infectious foci reduction) in both MA-104 and HT-29 cell lines. Furthermore, studies using a BALB/c mouse model have proved that this strain provides preliminary in vivo protection against rotavirus infection. The strain has been deposited in the Spanish Type Culture Collection under the accession number CECT 7210. This novel strain has the main properties required of a probiotic, such as resistance to gastrointestinal juices, biliary salts, NaCl, and low pH, as well as adhesion to intestinal mucus and sensitivity to antibiotics. The food safety status has been confirmed by the absence of undesirable metabolite production and in acute ingestion studies of mice. Overall, these results demonstrate that Bifidobacterium longum subsp. infantis CECT 7210 can be considered a probiotic able to inhibit rotavirus infection.
authors: Laboratorios Ordesa S.L., Parc Cientific de Barcelona, C/Baldiri Reixac 15-21, 08028 Barcelona, Spain. josea.moreno@ordesa.es
authors: José Antonio Moreno
authors: JA
authors: Muñoz
authors: Empar
authors: E
authors: Chenoll
authors: Beatriz
authors: B
authors: Casinos
authors: Esther
authors: E
authors: Bataller
authors: Daniel
authors: D
authors: Ramón
authors: Salvador
authors: S
authors: Genovés
authors: Rebeca
authors: R
authors: Montava
authors: Juan Manuel
authors: JM
authors: Ribes
authors: Javier
authors: J
authors: Buesa
authors: Joan
authors: J
authors: Fàbrega
authors: Montserrat
authors: M
authors: Rivero
mesh_terms: D000818:Animals; D000898:Antibiosis; D001644:Bifidobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002460:Cell Line; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D059022:Food Safety; D006801:Humans; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019936:Probiotics; D012401:Rotavirus / Q000254:growth & development*; D012400:Rotavirus Infections / Q000517:prevention & control*; D017422:Sequence Analysis, DNA; D014779:Virus Replication
chemical_list: D004269:DNA, Bacterial