title: Fabrication of cisplatin-loaded poly(lactide-co-glycolide) composite microspheres for osteosarcoma treatment.
abstract: OBJECTIVE
  To reduce the toxicity and achieve a sustainable and controllable release of cisplatin (CDDP).
  
  
  METHODS
  CDDP was loaded onto Fe5 (Fe(3+) doped hydroxyapatite at atomic ratio of Fe(added)/Ca(added) = 5%) nanoparticles through surface adsorption. Subsequently, CDDP-loaded Fe5 nanoparticles (CDDP-Fe5) and/or CDDP were encapsulated into poly(lactide-co-glycolide) (PLGA) microspheres using oil-in-water single emulsion. Drug release profiles and degradation behaviors were monitored.
  
  
  RESULTS
  CDDP-Fe5 demonstrated a high initial burst (42% on day 1) and short release time (25 days) as CDDP was directly released from Fe5 nanoparticles. CDDP-Fe5 encapsulated within the PLGA microspheres revealed a lower initial burst (23% on day 1) and longer release time (55 days) than CDDP-Fe5. Compared with PLGA microspheres containing only CDDP, which showed typical biphasic release manner, microspheres with CDDP-Fe5 and CDDP demonstrated a nearly linear release after the initial burst. Fe5 and CDDP delayed microsphere degradation. All samples became porous, disintegrated, fused, and formed pellets at the end of the study.
  
  
  CONCLUSIONS
  Fe5/PLGA composite microspheres showed favorable CDDP release behavior compared to microspheres composed of polymer alone, suggesting its potential as a new CDDP formulation.
authors: Division of Bioengineering School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Dr., Block N1.3, Singapore, 637457, Singapore.
authors: Yan
authors: Y
authors: Li
authors: Sierin
authors: S
authors: Lim
authors: Chui Ping
authors: CP
authors: Ooi
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002945:Cisplatin / Q000008:administration & dosage*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D012516:Osteosarcoma / Q000188:drug therapy; D011098:Polyglactin 910 / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D005290:Ferric Compounds; D011098:Polyglactin 910; D017886:Durapatite; D002945:Cisplatin