title: Lactic acid bacteria from chicken carcasses with inhibitory activity against Salmonella spp. and Listeria monocytogenes.
abstract: This study was conducted to isolate psychrotrophic lactic acid bacteria (LAB) from chicken carcasses with inhibitory activity against strains of Salmonella spp. and Listeria monocytogenes. A total of 100 broiler samples were examined for the presence of LAB. Ninety-two LAB isolates that showed antimicrobial effects against Salmonella spp. and L. monocytogenes were further analysed to examine their LAB (Gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (ability to grow at 7 °C). Fifty isolates were further selected and identified initially using standard biochemical tests in miniature (Micro-kits API CH 50) and then by sequencing of the 16s-23s rRNA gene boundary region (Intergenic Spacer Region). By molecular identification, these isolates were classified into 5 different LAB species: Lactobacillus salivarius, Lactobacillus reuteri, Lactobacillus johnsonii, Pediococcus acidilactici, and Lactobacillus paralimentarius. None of the isolates produced tyramine or histamine.
authors: Department of Hygiene and Technology of Foods of Animal Origin, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. jsakarid@vet.auth.gr
authors: I
authors: I
authors: Sakaridis
authors: N
authors: N
authors: Soultos
authors: C I
authors: CI
authors: Dovas
authors: E
authors: E
authors: Papavergou
authors: I
authors: I
authors: Ambrosiadis
authors: P
authors: P
authors: Koidis
mesh_terms: D000818:Animals; D000898:Antibiosis; D001679:Biogenic Amines / Q000096:biosynthesis; D002645:Chickens / Q000382:microbiology; D007777:Lactobacillaceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000254:growth & development*; D008461:Meat Products / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000737:chemistry; D012475:Salmonella / Q000254:growth & development*
chemical_list: D001679:Biogenic Amines; D012335:RNA, Ribosomal