title: Genetic characterization of an elite coffee germplasm assessed by gSSR  and  EST-SSR markers.
abstract: Coffee is one of the main agrifood commodities traded  worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian  agricultural production, generating a revenue of US$6 billion. Despite the  importance of coffee production in Brazil, it is supported by a narrow genetic  base, with few accessions. Molecular differentiation and diversity of a coffee  breeding program were assessed with gSSR and EST-SSR markers. The study  comprised 24 coffee accessions according to their genetic origin: arabica accessions  (six traditional genotypes of C. arabica), resistant arabica (six leaf  rust-resistant C. arabica genotypes with introgression of Híbrido de  Timor), robusta (five C. canephora genotypes), Híbrido de Timor (three C.  arabica x C. canephora), triploids (three C. arabica x C.  racemosa), and racemosa (one C. racemosa). Allele and polymorphism  analysis, AMOVA, the Student t-test, Jaccard's dissimilarity  coefficient, cluster analysis, correlation of genetic distances, and  discriminant analysis, were performed. EST-SSR markers gave 25 exclusive  alleles per genetic group, while gSSR showed 47, which will be useful for  differentiating accessions and for fingerprinting varieties. The gSSR markers  detected a higher percentage of polymorphism among (35% higher on average) and  within (42.9% higher on average) the genetic groups, compared to EST-SSR  markers. The highest percentage of polymorphism within the genetic groups was  found with gSSR markers for robusta (89.2%) and for resistant arabica (39.5%).  It was possible to differentiate all genotypes including the arabica-related  accessions. Nevertheless, combined use of gSSR and EST-SSR markers is  recommended for coffee molecular characterization, because EST-SSRs can provide  complementary information.
authors: Universidade Federal do Paraná, Campus Palotina, Palotina, PR, Brazil.
authors: R F
authors: RF
authors: Missio
authors: E T
authors: ET
authors: Caixeta
authors: E M
authors: EM
authors: Zambolim
authors: G F
authors: GF
authors: Pena
authors: L
authors: L
authors: Zambolim
authors: L A S
authors: LA
authors: Dias
authors: N S
authors: NS
authors: Sakiyama
mesh_terms: D000483:Alleles; D040503:Coffea / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D005819:Genetic Markers; D005838:Genotype; D011003:Ploidies; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers