RECK overexpression decreases invasive potential in prostate cancer cells.
BACKGROUND
RECK is a tumor suppressor which inhibits metastasis and angiogenesis. Based on RECK expression in prostate cancer tissue and cell lines, our aim was to investigate functional relevance of RECK for prostate carcinoma.


METHODS
RECK protein levels were determined by Western blotting in the human prostate cell lines BPH-1, DU-145, LNCaP, PC-3, and in tissue of 12 normal/tumor matches of patients after radical prostatectomy. Functional characteristics of DU-145 cells with stable RECK overexpression included proliferation, invasion, regulation of matrix metalloproteinases MMP-2, MMP-9, and MMP-14 measured by zymography (MMP-2 and -9) or commercially available assays.


RESULTS
RECK was expressed in cell lines and tissue with a significant decrease in malignant tissue (P = 0.002). RECK overexpression caused an up to 80% decrease in invasion for DU-145 cells (P < 0.001) and a decrease of pro-MMP-9 (42%) and of pro-/active MMP-14 (up to 53% of control). Proliferation was not affected by RECK overexpression.


CONCLUSIONS
The considerable anti-invasive potential of RECK points to new therapeutic possibilities for prostate cancer.
Department of Urology, Charité - Universitätsmedizin Berlin, Berlin, Germany. anja.rabien@charite.de
Anja
A
Rabien
Bettina
B
Ergün
Andreas
A
Erbersdobler
Klaus
K
Jung
Carsten
C
Stephan
D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D058851:GPI-Linked Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
D058851:GPI-Linked Proteins; C115460:RECK protein, human