Method of simultaneous stir bar sorptive extraction of phenethylamines and THC metabolite from urine.
Stir bar sorptive extraction is a technique used for extracting target substances from various aqueous matrixes such as environmental water, food, and biological samples. This type of extraction is carried out by rotating a coated stir bar is rotated in the sample solution. In particular, Twister bar is a commercial stir bar that is coated with polydimethylsiloxane (PDMS) and used to perform sorptive extraction. In this study, we developed a method for simultaneous detection of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, and a Δ(9)-tetrahydrocannabiniol (THC) metabolite in human urine. For extracting the target analytes, the Twister bar was simply stirred in the sample in the presence of a derivatizing agent. Using this technique, phenethylamines and the acidic THC metabolite can be simultaneously extracted from human urine. This method also enables the extraction of trace amounts of these substances with good reproducibility and high selectivity. The proposed method offers many advantages over other extraction-based approaches and is therefore well suited for screening psychoactive substances in urine specimens.
Forensic Science Laboratory, Kumamoto Prefectural Police Headquarters, Kumamoto, Japan. donuts5079@s4.kcn-tv.ne.jp
Yoshiyuki
Y
Goto
Shiho
S
Takeda
Toshinori
T
Araki
Takayuki
T
Fuchigami
D000327:Adsorption; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine*; D006801:Humans; D013287:Illicit Drugs / Q000378:metabolism / Q000652:urine*; D016014:Linear Models; D010627:Phenethylamines / Q000302:isolation & purification / Q000378:metabolism* / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013010:Sonication; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods*; D053719:Tandem Mass Spectrometry
D013287:Illicit Drugs; D010627:Phenethylamines; D013759:Dronabinol