A direct technique for magnetic functionalization of living human cells.
Functionalized living cells are regarded as effective tools in directed cell delivery and tissue engineering. Here we report the facile functionalization of viable isolated HeLa cells with superparamagnetic cationic nanoparticles via a single-step biocompatible process. Nanoparticles are localized on the cellular membranes and do not penetrate into the cytoplasm. The magnetically responsive cells are viable and able to colonize and grow on substrates. Magnetically facilitated microorganization of functionalized cells into viable living clusters is demonstrated. We believe that the technique described here may find a number of potential applications in cell-based therapies and in development of whole-cell biosensors.
Biomaterials and Nanomaterials Group, Faculty of Biology and Soil, Kazan (Idel buye/Volga region) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, 420008, RF.
Maria R
MR
Dzamukova
Alsu I
AI
Zamaleeva
Dilara G
DG
Ishmuchametova
Yuri N
YN
Osin
Andrey P
AP
Kiyasov
Danis K
DK
Nurgaliev
Olga N
ON
Ilinskaya
Rawil F
RF
Fakhrullin
D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D002412:Cations / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
D001672:Biocompatible Materials; D002412:Cations; D005290:Ferric Compounds; C000499:ferric oxide