Evaluation of dried blood spot sampling following cassette dosing in drug discovery.
BACKGROUND
Dried blood spot (DBS) sampling has received growing interest mainly in regulatory preclinical and clinical studies while not routinely used in exploratory discovery pharmacokinetic screening. An intravenous bolus cassette dose of six compounds in rats followed by hemolyzed blood sample (HBS) and DBS sampling was evaluated in this study.


RESULTS
A sensitive liquid chromatography tandem mass spectrometry method was developed and qualified for the simultaneous determination of six compounds in rat whole blood using DBS or HBS techniques. The concentrations obtained from DBS samples matched well with those from HBS for each individual compound.


CONCLUSIONS
This study demonstrated the applicability of DBS sampling for cassette dosing in discovery pharmacokinetics screening.
Drug Metabolism & Pharmacokinetics, Genentech Inc., South San Francisco, CA 94080, USA.
Xiaorong
X
Liang
Lulu
L
Yang
Leonid
L
Berezhkovskiy
Bianca
B
Liederer
Trung
T
Nguyen
Wei
W
Jia
Michelle
M
Schweiger
Brian
B
Dean
Yuzhong
Y
Deng
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing; D004353:Drug Evaluation, Preclinical; D006571:Heterocyclic Compounds / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
D006571:Heterocyclic Compounds