CD38: a NAADP degrading enzyme.
The role of the multifunctional enzyme CD38 in formation of the Ca(2+)-mobilizing second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) was investigated. Gene silencing of CD38 did neither inhibit NAADP synthesis in intact Jurkat T cells nor in thymus or spleen obtained from CD38 knock out mice. In vitro, both NAADP formation by base-exchange and degradation to 2-phospho adenosine diphosphoribose were efficiently decreased. Thus in vivo CD38 appears to be a NAADP degrading rather than a NAADP forming enzyme, perhaps avoiding desensitizing NAADP levels in intact cells.
Department of Biochemistry and Signal Transduction, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
Frederike
F
Schmid
Sören
S
Bruhn
Karin
K
Weber
Hans-Willi
HW
Mittrücker
Andreas H
AH
Guse
D051997:ADP-ribosyl Cyclase 1 / Q000235:genetics* / Q000378:metabolism; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020868:Gene Silencing; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009249:NADP / Q000031:analogs & derivatives* / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism
D008562:Membrane Glycoproteins; D000246:Adenosine Diphosphate Ribose; D009249:NADP; C024376:NAADP; C498155:Cd38 protein, mouse; D051997:ADP-ribosyl Cyclase 1; D002118:Calcium