Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylane 133, 9820 Merelbeke, Belgium. jella.wauters@ugent.be
Jella
J
Wauters
Thierry
T
Franck
Frederik
F
Pille
Ann
A
Martens
Kristel
K
Demeyere
Stanislas
S
Sys
Didier
D
Serteyn
Frank
F
Gasthuys
Evelyne
E
Meyer
D000818:Animals; D004396:Coloring Agents; D005434:Flow Cytometry / Q000662:veterinary*; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006736:Horses / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000378:metabolism*
D004396:Coloring Agents; D009195:Peroxidase