Angiotensin II induces extracellular matrix metalloproteinase inducer expression via an AT1R dependent pathway in aortic atherosclerotic plaque in apolipoprotein E knockout mice.
The pathogenesis of acute coronary syndrome is rupture of vulnerable plaque. Extracellular matrix metalloproteinase inducer (EMMPRIN) is reported to have a important role in the destabilization of atheroma.


OBJECTIVE
this investigation examined the effect of angiotensin II (Ang II) on EMMPRIN expression in atherosclerotic plaques in ApoE knockout mice.


METHODS
ApoE knockout mice were fed a high fat diet to establish an atherosclerosis model then intervention was made with Ang II and valsartan. EMMPRIN gene and its protein expression were measured by real-time PCR immunohistochemistry, and Western blot.


RESULTS
EMMPRIN gene and protein expression intervened with Ang II were significantly increased; valsartan could inhibit the effect of Ang II.


CONCLUSIONS
Ang II up-regulated EMMPRIN expression in atherosclerotic plaque via AT1R, and valsartan could inhibit the effect of Ang II.
Department of Cardiology, Kunming General Hospital of Chengdu Military Area, Yunnan, China. doctorlxyang@163.com
Li-xia
LX
Yang
Zhi-hua
ZH
Yang
Rui-wei
RW
Guo
Jin-shan
JS
Ye
Hong
H
Liu
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000473:pathology*; D001057:Apolipoproteins E / Q000172:deficiency* / Q000378:metabolism; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D051379:Mice; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013194:Staining and Labeling
D048868:Adaptor Proteins, Signal Transducing; C119712:Agtrap protein, mouse; D001057:Apolipoproteins E; C497887:Bsg protein, mouse; D008055:Lipids; D012333:RNA, Messenger; D000804:Angiotensin II; D051926:Basigin