Selection for tetracycline-resistant Chlamydia suis in treated pigs.
The aim of this study was to investigate Chlamydia suis in a pig farm with an outbreak of conjunctivitis and diarrhea. Eye swabs and pooled fecal samples were investigated for the presence of C. suis by real-time PCR and ArrayTube microarray. Samples positive for C. suis by ArrayTube microarray assay were further tested for the presence of the tet(C) resistance gene by PCR. In the first examination, C. suis was identified in 12 six-week-old pigs showing conjunctivitis. Of these, the tet(C) gene-coding region was amplified in one pooled fecal sample and one eye swab, respectively. After oral treatment with tetracycline, clinical symptoms disappeared. Subsequently, all eye swabs investigated from 10 healthy pigs were positive for C. suis and the tet(C) gene-coding region. The present study reports rapid selection for tetracycline-resistant C. suis after antibiotic treatment.
Institute for Veterinary Pathology, University of Zurich, Vetsuisse Faculty, Winterthurerstrasse 268, CH-8057 Zurich, Switzerland. n.borel@access.uzh.ch
Nicole
N
Borel
Nadine
N
Regenscheit
Antonietta
A
Di Francesco
Manuela
M
Donati
Jenny
J
Markov
Yvonne
Y
Masserey
Andreas
A
Pospischil
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D002689:Chlamydia / Q000187:drug effects* / Q000302:isolation & purification; D003231:Conjunctivitis / Q000382:microbiology / Q000662:veterinary*; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005123:Eye / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D034421:Sus scrofa / Q000382:microbiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013753:Tetracycline Resistance; D013754:Tetracyclines / Q000494:pharmacology / Q000627:therapeutic use
D000900:Anti-Bacterial Agents; D013754:Tetracyclines