Rapid isolation of adipose tissue-derived stem cells by the storage of lipoaspirates.
OBJECTIVE
This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs).


METHODS
Aliquots (10 g) of the lipoaspirates were stored at 4°C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed.


RESULTS
When the lipoaspirates were stored at 4°C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1×10(14) fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells.


CONCLUSIONS
ASCs isolated from lipoaspirates and stored for 24 hours at 4°C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4°C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
Department of Hematology-Oncology, Wonju College of Medicine, Yonsei University, Wonju, Korea.
Young Woo
YW
Eom
Jong Eun
JE
Lee
Mal Sook
MS
Yang
In Keun
IK
Jang
Hyo Eun
HE
Kim
Doo Hoon
DH
Lee
Young Jin
YJ
Kim
Won Jin
WJ
Park
Jee Hyun
JH
Kong
Kwang Yong
KY
Shim
Jong In
JI
Lee
Hyun Soo
HS
Kim
D015720:5'-Nucleotidase / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D000071063:Endoglin; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D024510:Muscle Development / Q000235:genetics / Q000502:physiology; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism; D055815:Young Adult
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; D015720:5'-Nucleotidase