[Biotransformation of 4-methyl-7-allyloxy coumarin by transgenic hairy roots of Polygonum multiflorum].
OBJECTIVE
To synthesis 4-methyl-7-allyloxy coumarin by Williamson etherification from 4-methyl-7-hydroxy coumarin and apply as a substrate in hairy roots of Polygonum multiflorum.


METHODS
The synthesis reaction of 4-methyl-7-allyloxy coumarin used the allyl bromide and potassium carbonate as catalysts, and acetone as solvent reacted for 17 hours, then the product was isolated. 4-methyl-7-allyloxy coumarin was added into the media of supension transgenic hairy roots of Polygonum multiflorum which had been precultured for 8 d, and then co-cultured for another 7 d. The biotransformation products were detected by TLC and HPLC and isolated by various chromatographic methods.


RESULTS
Two biotransformation products, 4-methyl-7-hydroxy coumarin and 4-methyl-coumarin-7-O-beta-D-glucoside were isolated and identified.


CONCLUSIONS
Hairy roots of Polygonum multiflorum contains not only glycosyltransferase but also hydrolysis enzymes.
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Yan
Y
Liang
Wen-juan
WJ
Deng
Liang-bin
LB
Zhou
Chun-yan
CY
Yan
Rong-min
RM
Yu
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques / Q000379:methods; D005960:Glucosides / Q000378:metabolism; D006923:Hymecromone / Q000031:analogs & derivatives* / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D028502:Polygonum / Q000235:genetics / Q000378:metabolism*
D005960:Glucosides; D006923:Hymecromone