Suitability of an open automated nucleic acid extractor for high-throughput plasma HIV-1 RNA quantitation in Gabon (Central Africa).
Nucleic acid extraction using the open automated EZ1 (Qiagen) instrument, in combination with the Generic HIV Viral Load assay, gave highly concordant HIV-1 RNA viral load results among 181 Gabonese subjects infected with HIV-1, compared to those obtained when performing a manual extraction. Since people living with HIV-1 are being treated with antiretrovirals in Gabon, this automated extraction technique represents an excellent technical method for high-throughput monitoring of HIV-1 RNA viral load.
UMI 233, Institut de Recherche pour le Développement (IRD), University of Montpellier, Montpellier, France.
Florian
F
Liégeois
Vanina
V
Boué
Augustin
A
Mouinga-Ondémé
Doris Kenfack
DK
Lékané
Delis
D
Mongo
Jeanne
J
Sica
François
F
Rouet
D001331:Automation / Q000379:methods*; D005681:Gabon; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D019562:Viral Load / Q000379:methods*
D012367:RNA, Viral