Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis.
Dynamic alterations in chromatin configuration occur in mammalian oocytogenesis. Based on chromatin configuration patterns, fully grown oocytes are classified into two types. One is surrounded nucleolus (SN)-type and the other is nonsurrounded nucleolus (NSN)-type oocytes. Although chromatin condensation during the transition from NSN- to SN-type oocytes is a prerequisite for normal early embryonic development, the molecular mechanisms remain unclear. In this study, we analyzed the role of DPPA3 (also known as PGC7/Stella) in this transition using Dppa3-null oocytes. The NSN-to-SN transition was significantly impaired, and transcriptional repression was incomplete in the Dppa3-null oocytes. Additionally, we revealed that prior transcriptional repression was necessary for the NSN-to-SN transition. These findings demonstrate that DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation.
Graduate School of Frontier Biosciences, Osaka University, Japan.
Yu-Jung
YJ
Liu
Toshinobu
T
Nakamura
Toru
T
Nakano
D000818:Animals; D002466:Cell Nucleolus / Q000502:physiology; D002843:Chromatin / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone; D005260:Female; D006570:Heterochromatin / Q000502:physiology; D006657:Histones / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; C462180:Dppa3 protein, mouse; D006570:Heterochromatin; D006657:Histones; D012097:Repressor Proteins