Controlled release of DNA from photoresponsive hyperbranched polyglycerols with oligoamine shells.
Two photo-responsive core/shell nanoparticles based on hyperbranched polyglycerol (hPG) are synthesized for controlled release of DNA. The shell is composed either of bis-(3-aminopropyl)methylamine (AMPA) or pentaethylenehexamine (PEHA) derivatives and is attached to the hPG core with a photo-responsive o-nitrobenzyl linker. Ethidium bromide displacement assay, gel electrophoresis, DLS, and ζ-potential measurements are performed with these nanoparticles. Photo-responsive changes within the carrier scaffold are investigated by irradiating the polymer solution with 350 nm monochromatic light. Fully covered APMA-shelled carriers are found to complex the DNA at an N/P ratio of 10 with an average size ranging from 54 to 78 nm depending on the degree of functionalization of the core.
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
Wiebke
W
Fischer
Mohiuddin A
MA
Quadir
Anna
A
Barnard
David K
DK
Smith
Rainer
R
Haag
D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D003692:Delayed-Action Preparations; D024202:Electrophoretic Mobility Shift Assay; D004705:Endocytosis; D018014:Gene Transfer Techniques; D005990:Glycerol / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008744:Methylamines / Q000737:chemistry*; D053758:Nanoparticles; D056951:Photoelectron Spectroscopy; D011073:Polyamines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D014466:Ultraviolet Rays
D003692:Delayed-Action Preparations; D008744:Methylamines; D011073:Polyamines; D011108:Polymers; C043941:polyglycerol; D004247:DNA; C031122:pentaethylenehexamine; D005990:Glycerol