Functional photoacoustic microscopy of pH.
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.
Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, USA.
Muhammad Rameez
MR
Chatni
Junjie
J
Yao
Amos
A
Danielli
Christopher P
CP
Favazza
Konstantin I
KI
Maslov
Lihong V
LV
Wang
D000818:Animals; D001578:Benzopyrans; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009284:Naphthols; D055096:Optical Devices; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D012235:Rhodamines
D001578:Benzopyrans; D005456:Fluorescent Dyes; D009284:Naphthols; D012235:Rhodamines; C068301:carboxy-seminaphthorhodaminefluoride