Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification.
The potential of 1-buthyl-3-methylpyridinium chloride, [Bmpy][Cl], as a pretreatment solvent for lignocellulosic biomasses, Bagasse and Eucalyptus, was investigated. The yields of regenerated biomasses ranged between 35% and 96%, and varied according to the pretreatment time, type of ionic liquid (IL) and biomass. The pretreatment of the biomass with [Bmpy][Cl] resulted in up to 8-fold increase in the cellulose conversion when compared with the untreated biomass. For a short pretreatment period (i.e., 10 min), [Bmpy][Cl] showed better performance than 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) with respect to the initial enzymatic saccharification rates. The increase in the reaction rates with [Emim][OAc] treatment was because of a reduction in the cellulose crystallinity. In contrast, a decrease in the crystallinity index was not clearly observed for the biomass pretreated with [Bmpy][Cl], and the enhancement of the enzymatic saccharification rates using this IL is presumably due to a reduction in the degree of polymerization of cellulose in the biomass.
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
Uju
Yasuhiro
Y
Shoda
Aya
A
Nakamoto
Masahiro
M
Goto
Wataru
W
Tokuhara
Yoshiyuki
Y
Noritake
Satoshi
S
Katahira
Nobuhiro
N
Ishida
Kazunori
K
Nakashima
Chiaki
C
Ogino
Noriho
N
Kamiya
D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry; D003460:Crystallization; D007093:Imidazoles / Q000494:pharmacology; D052578:Ionic Liquids / Q000494:pharmacology*; D008031:Lignin / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014961:X-Ray Diffraction
D007093:Imidazoles; D052578:Ionic Liquids; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase; C518739:1-ethyl-3-methylimidazolium