[Properties evaluation of collagen-hydroxyapatite-chondroitin sulfate-bone morphogenetic protein bone substitute material].
OBJECTIVE
To construct a new 3D porous bone substitute material with collagen, hydroxyapatite and chondroitin sulfate, which has the main components of nature bone and the cell growth factor BMP-2 with bone inductive ability.


METHODS
Collagen-hydroxyapatite-chondroitin sulfate scaffolds were prepared by chemical cross linking and freeze-drying, and bone morphogenetic protein (BMP) was incorporated into the scaffolds by adsorption. The bone substitute material was investigated by HE analysis, scanning electron microscope(SEM), electron spectroscopy for chemical analysis(ESCA), and X-ray diffraction(XRD). Rat mesenchymal stem cells (MSCs) were seeded into the scaffolds and cultured to form cell/scaffold (CS) constructs in vitro. The ectopic osteoinduction of the scaffolds were evaluated in vivo.


RESULTS
The bone substitute material had a porous 3D structure facilitating cells growing into it. Implanted into the muscle, the scaffolds were degraded with the forming of new bone.


CONCLUSIONS
Our Findings indicate that the bone substitute material has good biocompatibility and its attachment to CS could improve the adhesion and differentiation of cells.
Department of Orthopaedics, General Hospital of Chinese PLA, Beijing 100853, China.
Hao
H
Wang
Li-cheng
LC
Zhang
Tao
T
Shi
Qi
Q
Xiong
Pei-fu
PF
Tang
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage / Q000494:pharmacology*; D018786:Bone Substitutes / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008297:Male; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002809:Chondroitin Sulfates; D003094:Collagen; D017886:Durapatite