[Effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto].
OBJECTIVE
To investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.


METHODS
The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.


RESULTS
The QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).


CONCLUSIONS
QKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.
Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. lijing-c@hotmail.com
Lijing
L
Chen
Guoping
G
Liao
Yan
Y
Wang
Shuai
S
He
Zhongyi
Z
Zhang
D000293:Adolescent; D000328:Adult; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D003165:Complement System Proteins / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D006596:Hexosaminidases / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007267:Injections; D051381:Rats; D055815:Young Adult
D004365:Drugs, Chinese Herbal; C504928:Qingkailing; D003165:Complement System Proteins; D006596:Hexosaminidases