[Study on the hemostatic effects in raw and charred Mongolian drug Agi and its mechanism].
OBJECTIVE
To observe the effects of raw and charred Agi on hemostasis and its mechanism.


METHODS
The rabbit bleeding time was measured by traumatic hemorrhage test, and the clotting time was measured by tube test. The rabbit prothrombin time (PT), activated partial thormboplastia time (APTT), thrombin time (TT), fibrinogen (FIB), plasma recalcification time (PRT), euglobulin lysis time (ELT), max platelet aggregation rate (MPAR) were measured by solidification method, turbidimetry and tube test to analyze the effects of raw and charred Agi on rabbit coagulation-fibrinolysis system and platelet function.


RESULTS
The medium doses and high doses of raw Agi groups, all of the groups of charred Agi decreased rabbit BT obviously (P<0.01 or P<0.05); all of the groups of raw and charred Agi declined rabbits CT (P<0.01 or P<0.05). All of the doses groups of raw and charred Agi had no apparent influences on PT (P>0.05); the high dose of raw Agi group and all of the groups of charred Agi decreased APTT apparently (P<0.01 or P<0.05) and prolonged ELT (P<0.01 or P<0.05); the high doses groups of raw and charred Agi decreased TT apparently (P<0.01 or P<0.05); the medium and high doses groups of charred Agi increased FIB obviously (P<0.01 or P<0.05); the high doses group of charred Agi showed the decreased PRT significantly (P<0.05) and increased MPAR obviously (P<0.01).


CONCLUSIONS
Raw Agi can play its role in hemostasis and coagulation by affecting the intrinsic pathway of coagulation and fibrinolytic system. These effects are inhanced after processing drugs; moreover, the charred Agi could increase FIB and MPAR with promoting more in blood coagulation.
Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China. zhongboxiong@126.com
Bo-xiong
BX
Zhong
Wan
W
Zhang
Wei-zhi
WZ
Liu
Zong-ran
ZR
Pang
Jian
J
Cui
D000818:Animals; D019306:Artemisia / Q000737:chemistry; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010314:Partial Thromboplastin Time; D010946:Plants, Medicinal / Q000737:chemistry; D010974:Platelet Aggregation / Q000187:drug effects; D011517:Prothrombin Time; D011817:Rabbits; D013678:Technology, Pharmaceutical / Q000379:methods*; D013918:Thrombin Time
D004365:Drugs, Chinese Herbal; D006490:Hemostatics