Actinomadura geliboluensis sp. nov., isolated from soil.
A novel actinobacterium, strain A8036(T), isolated from soil, was investigated by using a polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generated spiral chains of spores with irregular surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9(H(6)) and MK-9(H(4)). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C(16:0), C(17:1)cis9, C(16:0), C(15:0) and 10-methyl C(17:0). Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain A8036(T) were Actinomadura meyerae DSM 44715(T) (99.23% similarity), Actinomadura bangladeshensis DSM 45347(T) (98.9%) and Actinomadura chokoriensis DSM 45346(T) (98.3%). However, DNA-DNA relatedness and phenotypic data demonstrated that strain A8036(T) could be clearly distinguished from the type strains of all closely related Actinomadura species. Strain A8036(T) is therefore considered to represent a novel species of the genus Actinomadura, for which the name Actinomadura geliboluensis sp. nov. is proposed. The type strain is A8036(T) ( = DSM 45508(T) = KCTC 19868(T)).
Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
Anil
A
Sazak
Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
Mustafa
M
Camas
German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
Cathrin
C
Spröer
German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
Hans-Peter
HP
Klenk
Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
Nevzat
N
Sahin
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D014421:Turkey
D002241:Carbohydrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil