[The influence of oxidised cholesterol derivatives on morphology of hepatocytes in rat's liver].
BACKGROUND
Products of oxidizing cholesterol-called oxysterols, are having different physicochemical properties and various from not-modified sterols, biological activity. Amongst characteristic effects of these connections are: stopping the activity of HMG-CoA reductase, antiproliferative action, apoptosis induction, changes in the structure and functioning of the cell membrane and influence on remodeling of the immunological system. Mechanisms of oxysterols influence on the cellular level include a broad spectrum of complicated biological activities and they haven't been examined to the end yet.


METHODS
In order to experiment execution the rat livers have been collected and the microscope sections have been made by standard technics, staining by haematoxylin and eosin (H&E). In each preparation 222 hepatic cells surrounding 10 central veins have been analysed and 222 hepatic cells occuring around 10 triads. The total surface ofcell, nucleus and cytoplasm have been taken for analysis.


RESULTS
In all researched cases the analysed parameters have been reduced in area liver traid and central vein. Decreasing of area value of cell, nucleus and cytoplasm have been observed.


CONCLUSIONS
On the basis of our examinations we determine, that oxysterols derived from the diet have influence on parenchymatous liver cells morphology in regions of hepatic triad and the central vein. We have concluded, that cytotoxic effect of oxysterols depend mainly on stopping the growth of these hepatic cells and lead to many disorders connected with the influence ofoxysterols, not only to cells but also to the all organism.
Katedra i Zakład Ogólnej Biologii Lekarskiej, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu Slaskiego Uniwersytetu Medycznego w Katowicach.
Anna
A
Mazur
Andrzej
A
Wiczkowski
Robert
R
Kubina
D000818:Animals; D002784:Cholesterol / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D011987:Receptors, Steroid / Q000378:metabolism; D000098825:Oxysterol Binding Proteins
D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases