Quantification of the cortical contribution to the NIRS signal over the motor cortex using concurrent NIRS-fMRI measurements.
Near-Infrared Spectroscopy (NIRS) measures the functional hemodynamic response occurring at the surface of the cortex. Large pial veins are located above the surface of the cerebral cortex. Following activation, these veins exhibit oxygenation changes but their volume likely stays constant. The back-reflection geometry of the NIRS measurement renders the signal very sensitive to these superficial pial veins. As such, the measured NIRS signal contains contributions from both the cortical region as well as the pial vasculature. In this work, the cortical contribution to the NIRS signal was investigated using (1) Monte Carlo simulations over a realistic geometry constructed from anatomical and vascular MRI and (2) multimodal NIRS-BOLD recordings during motor stimulation. A good agreement was found between the simulations and the modeling analysis of in vivo measurements. Our results suggest that the cortical contribution to the deoxyhemoglobin signal change (ΔHbR) is equal to 16-22% of the cortical contribution to the total hemoglobin signal change (ΔHbT). Similarly, the cortical contribution of the oxyhemoglobin signal change (ΔHbO) is equal to 73-79% of the cortical contribution to the ΔHbT signal. These results suggest that ΔHbT is far less sensitive to pial vein contamination and therefore, it is likely that the ΔHbT signal provides better spatial specificity and should be used instead of ΔHbO or ΔHbR to map cerebral activity with NIRS. While different stimuli will result in different pial vein contributions, our finger tapping results do reveal the importance of considering the pial contribution.
Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. lgagnon@nmr.mgh.harvard.edu
Louis
L
Gagnon
Meryem A
MA
Yücel
Mathieu
M
Dehaes
Robert J
RJ
Cooper
Katherine L
KL
Perdue
Juliette
J
Selb
Theodore J
TJ
Huppert
Richard D
RD
Hoge
David A
DA
Boas
D003198:Computer Simulation; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D008954:Models, Biological; D009044:Motor Cortex / Q000098:blood supply* / Q000378:metabolism; D059906:Neuroimaging / Q000379:methods; D019265:Spectroscopy, Near-Infrared
D006454:Hemoglobins