Effects of farm management practices and environmental factors on bulk tank milk antibodies against gastrointestinal nematodes in dairy farms across Canada.
Enzyme-linked immunosorbent assays (ELISAs) have been used as a diagnostic tool to quantify levels of gastrointestinal nematodes in dairy cattle by measuring Ostertagia ostertagi antibodies in milk. Higher levels of O. ostertagi antibodies measured by ELISA methods, referred to as optical density ratios (ODRs), are associated with decreased milk production in dairy cattle. On-farm management practices (e.g. pasturing techniques and anthelmintic usage) can influence the exposure of cattle to nematode infections and the magnitude of acquired worm burdens. Additionally, environmental and climatic factors, such as land elevation and precipitation, may also influence the levels of gastrointestinal parasitism. This repeated cross-sectional study investigated the effect of farm management practices and surrounding environmental factors on bulk tank (BT) ODRs in herds from provinces across Canada, and further examined the potential effects of various anthelmintic treatment protocols on BT ODRs. A total of 195 herds contributed an average of 3.5 BT samples between December 2003 and April 2005. The farm management practices were recorded from a questionnaire asking producers about their pasturing methods (confined, pastured, etc.), pasture sharing practices (e.g. mixing heifers with milking cows) and anthelmintic treatments. Environmental data were downloaded online from various governmental databases (e.g. Natural Resources Canada, Statistics Canada, Environment Canada, etc.). Statistical models, accounting for repeated measures (multiple BT ODRs for each farm) and for clustering of farms within a region (province or ecoregion), were used to analyze environmental and farm management data. Overall, the greater the exposure that heifers and milking cows had to pasture, the higher the levels of anti-parasite antibodies detected in BT samples. Treating the entire herd or treating milking cows at calving reduced BT ODR values. Farms in areas with higher number of rainy days, higher normalized difference vegetation index (NDVI) values, and lower land surface temperatures (LSTs), were also likely to have higher BT ODRs. Seasonal variation was such that late summer and early fall, when parasite load was at its highest, yielded larger BT ODRs. Due to the high clustering effect at the herd level, factors at the herd level (e.g. pasturing methods, anthelmintic administration) had a higher potential impact on bulk-tank measurements than the herd's surrounding environmental factors.
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, Canada. rvanderstich@upei.ca
Raphaël
R
Vanderstichel
Ian
I
Dohoo
Javier
J
Sanchez
Gary
G
Conboy
D000822:Animal Husbandry; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000969:Antinematodal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002170:Canada / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology / Q000517:prevention & control*; D003430:Cross-Sectional Studies; D003612:Dairying; D004777:Environment; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D041981:Gastrointestinal Tract; D008892:Milk / Q000469:parasitology; D009349:Nematode Infections / Q000453:epidemiology / Q000517:prevention & control / Q000662:veterinary*; D010028:Ostertagia / Q000276:immunology; D011795:Surveys and Questionnaires
D000909:Antibodies, Helminth; D000969:Antinematodal Agents