Indigestible dextrin is an excellent inducer for α-amylase, α-glucosidase and glucoamylase production in a submerged culture of Aspergillus oryzae.
α-Amylase activities of Aspergillus oryzae grown on dextrin or indigestible dextrin were 7·8 and 27·7 U ml(-1), respectively. Glucoamylase activities of the cultures grown on dextrin or indigestible dextrin were 5·4 and 301 mU ml(-1), respectively. The specific glucoamylase production rate in indigestible dextrin batch culture reached 1·35 U g DW(-1) h(-1). In contrast, biomass concentration of A. oryzae in indigestible dextrin culture was 35% of that in dextrin culture. Thus, the culture method using indigestible dextrin has the potential to improve amylolytic enzyme production and fungal fermentation broth rheology.
Research Laboratories for Brewing, Asahi Breweries, Ltd., 1-21, Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan. toshikazu.sugimoto@asahibeer.co.jp
Toshikazu
T
Sugimoto
Hiroshi
H
Shoji
D001236:Aspergillus oryzae / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003912:Dextrins / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D015533:Transcriptional Activation; D000516:alpha-Amylases / Q000096:biosynthesis*; D000520:alpha-Glucosidases / Q000096:biosynthesis*
D003470:Culture Media; D003912:Dextrins; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase