An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify (E)- and (Z)-diastereomers of α-asarone and β-asarone from Acorus tatarinowii Schott.
In this study, supercritical fluid extraction (SFE) was used to extract essential oil from Acorus tatarinowii Schott under the pressure of 25 MPa and temperature of 35°C. Two (E)- and (Z)-diastereomers of α-asarone and β-asarone were separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.2:1:0.3, v/v). The separation yielded a total of 39 mg of α-asarone and 463 mg of β-asarone from 1.0 g of essential oil in one-step separation with the purity of 98.9 and 99.1%, respectively, as determined by HPLC. The chemical structures of these compounds were identified by EI-MS, (1)H-, and (13)C-NMR.
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, P. R. China.
Daijie
D
Wang
Yanling
Y
Geng
Lei
L
Fang
Xikai
X
Shu
Jianhua
J
Liu
Xiao
X
Wang
Luqi
L
Huang
D029023:Acorus / Q000737:chemistry*; D000081248:Allylbenzene Derivatives; D000840:Anisoles / Q000737:chemistry* / Q000302:isolation & purification*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D004365:Drugs, Chinese Herbal; C012195:asarone