Arsenic accumulation and translocation in the submerged macrophyte Hydrilla verticillata (L.f.) Royle.
Worldwide contamination of arsenic in aquatic systems requires the development of a cost-effective, in situ phytoremediation technology. Hydrilla verticillata (L.f.) Royle, a submerged macrophyte widely distributed throughout the world, has the potential to effectively remove heavy metals from water. In order to understand the potential of H. verticillata for As phytofiltration and its impacts on As cycling in the water system, we investigated As accumulation, speciation and translocation in H. verticillata plants. Plant shoots showed a significant accumulation of As, with a maximum of >700 μg g(-1) dry weight (DW) after exposure to 20 μM arsenate [As(V)] or arsenite [As(III)] for 4d, with no significant differences between the As(V) and As(III) treatments (P>0.05). In addition, results of an in planta transport experiment showed that, after exposure of root and shoot to 2μM As(V) and As(III) for 4d, the bioconcentration factor (BCF) in roots for As(V) was almost twofold than that of As(III). Higher As BCFs in roots compared to shoots was also observed. Arsenic accumulated primarily in the cell walls of root cells (>73% of the total As in roots) and in the soluble parts of leaves (>60% of the total As in leaves). Regardless of the form of As supplied [As(III) or As(V)], As(V) was the dominant form in roots and As(III) was the dominant form in leaves. Further, basipetal translocation of As in this plant (≥17%) was markedly higher than acropetal translocation (≤3%). Because of accumulation of As in the shoot and immobilization of As below ground in roots, H. verticillata is a potential As phytofiltrator for bioremediation.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.
Pei-ying
PY
Xue
Chang-zhou
CZ
Yan
D001149:Arsenates / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism*; D018053:Arsenites / Q000378:metabolism; D001673:Biodegradation, Environmental; D031321:Hydrocharitaceae / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001149:Arsenates; D018053:Arsenites; D014874:Water Pollutants, Chemical; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid