In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography.
We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens.
Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, Milan, 20133, Italy. andreabassi@polimi.it
Andrea
A
Bassi
Luca
L
Fieramonti
Cosimo
C
D'Andrea
Marina
M
Mione
Gianluca
G
Valentini
D000818:Animals; D030801:Animals, Genetically Modified; D001808:Blood Vessels / Q000033:anatomy & histology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods*; D055620:Optical Phenomena; D011994:Recombinant Proteins / Q000235:genetics; D012212:Rheology / Q000379:methods; D041622:Tomography, Optical / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology* / Q000235:genetics
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins