Anti-inflammatory effects of fatty acids isolated from Chromolaena odorata.
OBJECTIVE
To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata (C. odorata).


METHODS
The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells.


RESULTS
Six fatty acids (S)-coriolic acid (1), (S)-coriolic acid methyl ester (2), (S)-15,16-didehydrocoriolic acid (3), (S)-15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide (6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-κB inhibition. Compound 2 was the most active with the IC(50) values of 5.22 and 5.73 μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities.


CONCLUSIONS
The fatty acid components in C. odorata with NF-κB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine. This study could also contribute to the better use of C. odorata for human health care.
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Tran Thi Hong
TT
Hanh
Dan Thi Thuy
DT
Hang
Chau
C
Van Minh
Nguyen Tien
NT
Dat
D000818:Animals; D002460:Cell Line; D036604:Chromolaena / Q000737:chemistry*; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
D005227:Fatty Acids; D016328:NF-kappa B; D010936:Plant Extracts; D009569:Nitric Oxide