DNA-polymer micelles as nanoparticles with recognition ability.
The Watson-Crick binding of DNA single strands is a powerful tool for the assembly of nanostructures. Our objective is to develop polymer nanoparticles equipped with DNA strands for surface-patterning applications, taking advantage of the DNA technology, in particular, recognition and reversibility. A hybrid DNA copolymer is synthesized through the conjugation of a ssDNA (22-mer) with a poly(ethylene oxide)-poly(caprolactone) diblock copolymer (PEO-b-PCl). It is shown that, in water, the PEO-b-PCl-ssDNA(22) polymer forms micelles with a PCl hydrophobic core and a hydrophilic corona made of PEO and DNA. The micelles are thoroughly characterized using electron microscopy (TEM and cryoTEM) and small-angle neutron scattering. The binding of these DNA micelles to a surface through DNA recognition is monitored using a quartz crystal microbalance and imaged by atomic force microscopy. The micelles can be released from the surface by a competitive displacement event.
Université de Toulouse, CNRS, Laboratoire des IMRCP, UMR 5623, 118 route de Narbonne, 31062 Toulouse Cedex 9, France.
Renée Mayap
RM
Talom
Gad
G
Fuks
Leonard
L
Kaps
Julian
J
Oberdisse
Christel
C
Cerclier
Cédric
C
Gaillard
Christophe
C
Mingotaud
Fabienne
F
Gauffre
D004247:DNA / Q000737:chemistry*; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D008823:Micelles; D009841:Oligonucleotides; D011091:Polyesters; D011108:Polymers; C458722:polyethylene oxide-polycaprolactone copolymer; D004247:DNA