Hepatic megalocytosis due to vanadium inhalation: participation of oxidative stress.
The aim of this study was to evaluate the morphological changes, liver function test (LFT), and oxidative stress damage caused by thiobarbituric acid reactive substances (TBARS), in mice exposed to vanadium via inhalation. Male CD-1 mice were exposed to vanadium pentoxide (V(2)O(5)) via inhalation (0.02 M), 1 hour twice a week for 6 weeks. At the end of the protocol, controls and exposed mice were killed to evaluate the changes. Histological analysis and LFT were performed to detect the damage. TBARS detection was assessed for oxidative stress. Inflammatory infiltration, binucleation, and meganucleus were detected in the liver of V(2)O(5)-exposed mice (p < 0.05). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were also significantly increased (p < 0.05). Lipid peroxidation was significantly higher in V(2)O(5)-exposed animals compared to controls (p < 0.05). V(2)O(5) exposure induced inflammation and cell damage detected by the increase in ALT and AST levels, as well as histological changes that suggest regenerative changes, such as binucleation and meganucleus.
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, México City, México.
Gumaro
G
Cano-Gutiérrez
Sandra
S
Acevedo-Nava
Abel
A
Santamaría
Mario
M
Altamirano-Lozano
María Concepción
MC
Cano-Rodríguez
Teresa I
TI
Fortoul
D000280:Administration, Inhalation; D000704:Analysis of Variance; D000818:Animals; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D006651:Histocytochemistry; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D019570:Inhalation Exposure; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D017968:Vanadium Compounds / Q000008:administration & dosage / Q000633:toxicity*
D017968:Vanadium Compounds; C066075:vanadium pentoxide