Impact of ultraviolet-B radiation on planktonic fish larvae: alteration of the osmoregulatory function.
Coastal marine ecosystems are submitted to variations of several abiotic and biotic parameters, some of them related to global change. Among them the ultraviolet-B (UV-B) radiation (UVBR: 280-320 nm) may strongly impact planktonic fish larvae. The consequences of an increase of UVBR on the osmoregulatory function of Dicentrarchus labrax larvae have been investigated in this study. In young larvae of D. labrax, as in other teleosts, osmoregulation depends on tegumentary ion transporting cells, or ionocytes, mainly located on the skin of the trunk and of the yolk sac. As early D. labrax larvae passively drift in the top water column, ionocytes are exposed to solar radiation. The effect of UVBR on larval osmoregulation in seawater was evaluated through nanoosmometric measurements of the blood osmolality after exposure to different UV-B treatments. A loss of osmoregulatory capability occured in larvae after 2 days of low (50 μWcm(-2): 4 h L/20 h D) and medium (80 μWcm(-2): 4 h L/20 h D) UVBR exposure. Compared to control larvae kept in the darkness, a significant increase in blood osmolality, abnormal behavior and high mortalities were detected in larvae exposed to UVBR from 2 days on. At the cellular level, an important decrease in abundance of tegumentary ionocytes and mucous cells was observed after 2 days of exposure to UVBR. In the ionocytes, two major osmoeffectors were immunolocalized, the Na+/K(+)-ATPase and the Na+/K+/2Cl- cotransporter. Compared to controls, the fluorescent immunostaining was lower in UVBR-exposed larvae. We hypothesize that the impaired osmoregulation in UVBR-exposed larvae originates from the lower number of tegumentary ionocytes and mucous cells. This alteration of the osmoregulatory function could negatively impact the survival of young larvae at the surface water exposed to UVBR.
AEO Team (Adaptation Ecophysiologique et Ontogenèse), UMR 5119 Ecosym UM2, CNRS, IRD, Ifremer, UM1, Université Montpellier 2, cc092, Pl. Eugène Bataillon, 34095 Montpellier, Cx 05, France. elliott.sucre@univ-montp2.fr
Elliott
E
Sucré
Francesca
F
Vidussi
Behzad
B
Mostajir
Guy
G
Charmantier
Catherine
C
Lorin-Nebel
D000818:Animals; D001492:Bass / Q000502:physiology*; D001522:Behavior, Animal / Q000528:radiation effects; D009994:Osmolar Concentration; D014466:Ultraviolet Rays; D014882:Water-Electrolyte Balance / Q000528:radiation effects*