Use of an exchange method to estimate the association and dissociation rate constants of cadmium complexes formed with low-molecular-weight organic acids commonly exuded by plant roots.
Organic acids released from plant roots can form complexes with cadmium (Cd) in the soil solution and influence metal bioavailability not only due to the nature and concentration of the complexes but also due to their lability. The lability of a complex influences its ability to buffer changes in the concentration of free ions (Cd); it depends on the association (, m mol s) and dissociation (, s) rate constants. A resin exchange method was used to estimate  and  (m mol s), which is the conditional estimate of  depending on the calcium (Ca) concentration in solution. The constants were estimated for oxalate, citrate, and malate, three low-molecular-weight organic acids commonly exuded by plant roots and expected to strongly influence Cd uptake by plants. For all three organic acids, the  and  estimates were around 2.5 10 m mol s and 1.3 × 10 s, respectively. Based on the literature, these values indicate that the Cd- low-molecular-weight organic acids complexes formed between Cd and low-molecular-weight organic acids may be less labile than complexes formed with soil soluble organic matter but more labile than those formed with aminopolycarboxylic chelates.
INRA, Transfert sol-plante et Cycles des Elements Mineraux dans les ecosystemes cultives, Villenave-d'Ornon, France. schneide@bordeaux.inra.fr
André
A
Schneider
Christophe
C
Nguyen
D002104:Cadmium / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D009930:Organic Chemicals / Q000737:chemistry*; D018517:Plant Roots / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000737:chemistry*
D009930:Organic Chemicals; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium