From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay.
Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.
SomaLogic, Inc., Boulder, Colorado, United States of America.
Stephan
S
Kraemer
Jonathan D
JD
Vaught
Christopher
C
Bock
Larry
L
Gold
Evaldas
E
Katilius
Tracy R
TR
Keeney
Nancy
N
Kim
Nicholas A
NA
Saccomano
Sheri K
SK
Wilcox
Dom
D
Zichi
Glenn M
GM
Sanders
D001331:Automation; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers / Q000032:analysis*; D016022:Case-Control Studies; D019937:Diagnostic Techniques and Procedures; D006801:Humans; D057230:Limit of Detection; D009696:Nucleic Acids / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D017186:Titrimetry
D015415:Biomarkers; D009696:Nucleic Acids; D009841:Oligonucleotides