Cell adhesion, spreading, and proliferation on surface functionalized with RGD nanopillar arrays.
In this paper, a method was introduced for the fabrication of vertically and spatially-controlled peptide nanostructures that enhance cell adhesion, proliferation, spreading on artificial surfaces. The RGD nanostructures with different heights were fabricated on gold surfaces by self-assembly technique through a nanoporous alumina mask composed of nanoscale-controlled pores. Pore diameter and spatial distribution were controlled by manipulating the pore widening time at a constant voltage during the mask fabrication process. Two-dimensional RGD nanodot, three-dimensional RGD nanorod, and RGD nanopillar arrays were carried out using various concentrations of RGD peptide solution, self-assembly times, and pore sizes, which were 74 nm, 63 nm, and 43 nm in diameter, respectively. The fabricated RGD nanodot, nanorod, and nanopillar arrays were utilized as a cell adhesion layer to evaluate the cell adhesion force, adhesion speed, spreading assay, and phosphorylation of cofilin protein in PC12, HeLa, and HEK293T normal cells. Among the three different nanostructures, RGD nanopillar arrays were found to be suitable for cellular attachment, spreading, and proliferation due to the proper arrangement of the RGD motif, which mimics in vivo conditions. Hence, our newly fabricated RGD nanostructured array can be successfully applied as a bio-platform for improving cellular functions and in in vitro tissue engineering.
Interdisciplinary Program of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, Republic of Korea.
Md
M
Abdul Kafi
Waleed Ahmed
WA
El-Said
Tae-Hyung
TH
Kim
Jeong-Woo
JW
Choi
D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D006367:HeLa Cells; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D009842:Oligopeptides / Q000737:chemistry*; D016716:PC12 Cells; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid