DFA III production from inulin with inulin fructotransferase in ultrafiltration membrane bioreactor.
An ultrafiltration membrane bioreactor was used for the production of DFA III from enzymatic conversion of inulin. Compared with the traditional batch reactor, the productivity and purity of DFA III could be markedly enhanced and product inhibition was removed and IFTase could be continuously used for six runs in the UF membrane bioreactor. When the substrate concentration was 100 g/L, the concentration of DFA III was about 78.4 g/L, while the productivity and purity of DFA III could attain about 2385 and 92%, respectively.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Hua
H
Hang
Wanmeng
W
Mu
Bo
B
Jiang
Meng
M
Zhao
Liuming Leon
LL
Zhou
Tao
T
Zhang
Ming
M
Miao
D001173:Arthrobacter / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism; D019149:Bioreactors; D004187:Disaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D006602:Hexosyltransferases / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism*; D014462:Ultrafiltration
D001426:Bacterial Proteins; D004187:Disaccharides; C087758:difructose anhydride III; D007444:Inulin; D006602:Hexosyltransferases