Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media.
An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown  on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation  of M. tuberculosis in acidfast bacilli positive cultures.
Infectious Disease Section, Department of Medicine, University of the Philippines-Philippine General Hospital, Manila, Philippines.
C F
CF
Ang
M A M
MA
Cajucom
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Y
Kim
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H
Bang
H
H
Lee
S N
SN
Cho
C S
CS
Montalban
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques; D003470:Culture Media; D012321:DNA-Directed RNA Polymerases; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; C510537:MPT64 protein, Mycobacterium tuberculosis; D011933:Reagent Kits, Diagnostic; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases