Electron transfer through RNA: chemical probing of dual distance dependence.
Electron transfer (ET) through RNA duplexes possessing 2'-O-pyrenylmethy uridine (Upy) and 5-bromouracil (BrU) as an electron donor and accepter set was investigated. Reductive decomposition of the BrU resulted from the ET over long distances (up to ten AU base pairs) was detected in the RNA conjugates. The RNA mediated ET from the pyrene to BrU showed dual distance dependence. This is well consistent with the previous observation for ET from Upy to nitrobenzene in RNA. In contrast, little or no reductive decomposition of the BrU was observed in the DNA conjugates when the Upy and BrU were separated by more than four AT base pairs.
Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Shosha, Himeji, Japan.
Tadao
T
Takada
Yumiko
Y
Otsuka
Mitsunobu
M
Nakamura
Kazushige
K
Yamana
D001976:Bromouracil / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004579:Electron Transport; D004583:Electrons; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
C527209:2'-O-pyrenylmethyluridine; D001976:Bromouracil; D012313:RNA; D004247:DNA; C006824:5-bromouridine; D014529:Uridine