A semi-empirical approach for predicting unobserved peptide MS/MS spectra from spectral libraries.
Spectral library searching is a promising alternative to sequence database searching in peptide identification from MS/MS spectra. The key advantage of spectral library searching is the utilization of more spectral features to improve score discrimination between good and bad matches, and hence sensitivity. However, the coverage of reference spectral library is limited by current experimental and computational methods. We developed a computational approach to expand the coverage of spectral libraries with semi-empirical spectra predicted from perturbing known spectra of similar sequences, such as those with single amino acid substitutions. We hypothesized that the peptide of similar sequences should produce similar fragmentation patterns, at least in most cases. Our results confirm our hypothesis and specify when this approach can be applied. In actual spectral searching of real data sets, the sensitivity advantage of spectral library searching over sequence database searching can be mostly retained even when all real spectra are replaced by semi-empirical ones. We demonstrated the applicability of this approach by detecting several known non-synonymous single-nucleotide polymorphisms in three large human data sets by spectral searching.
Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology, Hong Kong, PR China.
Yingwei
Y
Hu
Yunzi
Y
Li
Henry
H
Lam
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019295:Computational Biology; D030562:Databases, Protein; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D040901:Proteomics / Q000379:methods*; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019151:Peptide Library; D010455:Peptides