Serologic survey of humans for flavivirus West Nile in Southern Moravia (Czech Republic).
A serosurvey for West Nile virus (WNV) was carried out in 525 persons, using a plaque-reduction neutralization microtest (PRNmicroT) with Vero cells and Egyptian topotype Eg-101 strain as test virus. The blood sera were collected in four South-Moravian districts (Hodonin 44 persons, Breclav 102 persons, Znojmo 170 persons, Jihlava 209 persons) of the Czech Republic in the years 1988 and 1989, and stored at -20 degrees C. Antibodies to WNV were detected in only three humans (0.6% seropositivity): one person each in the districts of Hodonin (2.3% persons positive), Breclav (1.0% positive) and Jihlava (0.5% positive), with the titres of 1:64, 1:32, and 1:32, respectively. All the three sera were negative for antibody to tick-borne encephalitis virus. The results indicate that activity of WNV in southern Moravia was very low before 1990.
Institute of Vertebrate Biology, Academy of Sciences, Brno, Czech Republic. zhubalek@ivb.cz
Zdenek
Z
Hubálek
Bohumír
B
Kríz
Jirí
J
Halouzka
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005416:Flavivirus; D006801:Humans; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D010948:Viral Plaque Assay; D014901:West Nile Fever / Q000097:blood* / Q000453:epidemiology*; D014902:West Nile virus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D055815:Young Adult
D000914:Antibodies, Viral