Comparative study on occurrence of class A and class C β -lactamase genes and their co-occurrence in Indian Enterobacteriaceae during years 2009 and 2010.
OBJECTIVE
To determine the occurrence of class A and class C β-lactamase genes and their co-occurrence in Indian Enterobacteriaceae.


METHODS
52 third generation cephalosporin resistant isolates were phenotypically detected by combination disk method and screened by PCR to identify class A and class C type β-lactamase genes.


RESULTS
Of the 52 isolates, 94.2% (49) were found harboring any of the bla(ESBL(s)). bla(CTX-M), bla(SHV) and bla(TEM) were present in 82.6% (43/52), 59.6% (31/52), and 42.3% (22/52) isolates, respectively. Of the 49 ESBL positive isolates 57.1% (28/49) showed co-occurrence of bla(ampC) with bla(ESBL(s)). On the contrary, the collection from 2009 showed their co-occurrence in 81.4% isolates.


CONCLUSIONS
The comparative study shows a downward trend for co-existence of bla(ESBL(s)) with bla(ampC) from 2009 to 2010. Further large scale studies are needed to address the co-occurrence of class A and class C β-lactamases in India and the resistance trend occurring over a period of time.
Section of Antimicrobial Agents & Drug Resistance Research and Molecular Biology, Department of Microbiology, Jawaharlal Nehru Medical College & Hospital, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India.
Anuradha
A
Singh
Mohd
M
Shahid
Farrukh
F
Sobia
Umesh
Haris M
HM
Khan
D018550:Cephalosporin Resistance / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007194:India; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases