Anti-idiotypic single chain mimicking CA125 linked with tuftsin provides             protective immunity against ovarian cancer in mice.
The activation of effector cells by bifunctional proteins to kill target             cells has great potential in the treatment of cancer. In this study, a recombinant             fusion protein composed of an anti‑idiotypic single chain mimicking CA125 connected             with tuftsin by an artificial linker was constructed. The fusion protein was found             to manifest a number of biological activities, including activation of macrophages             and stimulation of the T-cell response against cancer. Compared with single‑chain             Fv without tuftsin, the fusion protein showed stronger immunogenicity triggering             humoral and cellular immune responses in mice. Fusion of tuftsin to scFv resulted             in enhanced production of anti-anti-idiotypic antibodies and T-cell response.             Protection against tumor challenges may be achieved in animals immunized with             fusion protein. These results raise the possibility of employing cancer immunotherapy             by administration of fusion proteins composed of anti‑idiotypic antibodies and             tuftsin.
State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, PR China.
Wei
W
Yuan
Guimin
G
Xia
Chen
C
Zhao
Chenguang
C
Sui
Jie
J
Ma
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D018394:CA-125 Antigen / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use; D005260:Female; D008262:Macrophage Activation; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000627:therapeutic use; D057127:Single-Chain Antibodies / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous; D014405:Tuftsin / Q000235:genetics / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D018394:CA-125 Antigen; D019496:Cancer Vaccines; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; D014405:Tuftsin