Oxidative modification of patient's plasma proteins and its role in pathogenesis of multiple sclerosis.
BACKGROUND
Oxidative stress plays an important role in multiple sclerosis (MS).


OBJECTIVE
The present study was designed to evaluate the modifications of plasma proteins by estimation markers of oxidative/nitrosative stress: carbonyl groups and 3-nitrotyrosines (3-NT) levels in relapsing-remitting (RR) (n=10) and secondary progressive (SP) (n=10) clinical course of multiple sclerosis. Moreover, we estimated the level of uric acid (UA) in plasma of MS patients.


RESULTS
Compared to controls (n=10), the levels of carbonyl groups in plasma proteins were elevated (P<0.0001) as well in RRMS as in SPMS. The highest concentration of 3-NT was observed in plasma proteins obtained from SPMS patients (P<0.0005). The level of uric acid in plasma was significantly lower in RRMS (P<0.0001) than SPMS.


CONCLUSIONS
This is the first report which presented differences between SPMS and RRMS patients in 3-NT and protein carbonyl groups in plasma proteins.
Neurorehabilitation Ward, III General Hospital in Lodz, Lodz, Milionowa 14, Poland. betty.miller@interia.pl
Elżbieta
E
Miller
Anna
A
Walczak
Joanna
J
Saluk
Michał B
MB
Ponczek
Ireneusz
I
Majsterek
D000328:Adult; D001798:Blood Proteins / Q000737:chemistry*; D001812:Blood-Brain Barrier; D002244:Carbon / Q000737:chemistry; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D020528:Multiple Sclerosis, Chronic Progressive / Q000097:blood* / Q000378:metabolism; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry*; D017382:Reactive Oxygen Species; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D014527:Uric Acid / Q000097:blood
D001798:Blood Proteins; D017382:Reactive Oxygen Species; D014527:Uric Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D002244:Carbon; D010100:Oxygen