Visualizing mechanical tension across membrane receptors with a fluorescent sensor.
We report a fluorescence-based turn-on sensor for mapping the mechanical strain exerted by specific cell-surface proteins in living cells. The sensor generates force maps with high spatial and temporal resolution using conventional fluorescence microscopy. We demonstrate the approach by mapping mechanical forces during the early stages of regulatory endocytosis of the ligand-activated epidermal growth factor receptor (EGFR).
Department of Chemistry, Emory University, Atlanta, Georgia, USA.
Daniel R
DR
Stabley
Carol
C
Jurchenko
Stephen S
SS
Marshall
Khalid S
KS
Salaita
D001696:Biomechanical Phenomena / Q000502:physiology; D001710:Biotin / Q000737:chemistry; D002232:Carbocyanines; D004705:Endocytosis / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008465:Mechanoreceptors / Q000502:physiology*; D008856:Microscopy, Fluorescence; D009711:Nucleotides; D010713:Phosphatidylcholines / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000502:physiology; D010766:Phosphorylation; D011092:Polyethylene Glycols / Q000737:chemistry; D012235:Rhodamines
C569686:Alexa Fluor 647; D002232:Carbocyanines; D008051:Lipid Bilayers; D009711:Nucleotides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C554276:QSY 21 compound; D012235:Rhodamines; D011092:Polyethylene Glycols; D001710:Biotin; C020888:1,2-dielaidoylphosphatidylethanolamine; D066246:ErbB Receptors; C017251:1,2-oleoylphosphatidylcholine