Microfluidic devices with disposable enzyme electrode for electrochemical monitoring of glucose concentrations.
This article describes the fabrication of tube-like microchannels made of UV curable polymer on a glass substrate and the device assembling with a disposable enzyme-working electrode for high-sensitivity electrochemical detection. While both reference and counter electrodes are patterned on the surface of the glass substrate, the working electrode is flipped on the top of the channel with an open access, providing a face-to-face probing configuration. When the enzyme electrode is contaminated or degraded, it can be easily replaced by a new one, keeping the main body of the device and the detection schema unchanged. Using glucose oxidase-coated gold electrodes, we were able to determine a linear amperometry response to the glucose concentrations in the range of 2-16  mM. By replacing the as-prepared working electrode by the one after thermal treatments, we showed a much more degraded enzyme electrode activity, enabling efficient determination of the electrode quality as well as the whole process optimization.
Ecole Normale Supérieure, Paris, France.
Xin
X
Li
Fan
F
Zhang
Jian
J
Shi
Li
L
Wang
Jing-Hua
JH
Tian
Xiong-Tu
XT
Zhou
Lian-Mei
LM
Jiang
Li
L
Liu
Zhen-Jie
ZJ
Zhao
Pin-Gang
PG
He
Yong
Y
Chen
D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose