True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.
Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark.
Vibeke B
VB
Rudkjøbing
Trine R
TR
Thomsen
Morten
M
Alhede
Kasper N
KN
Kragh
Per H
PH
Nielsen
Ulla R
UR
Johansen
Michael
M
Givskov
Niels
N
Høiby
Thomas
T
Bjarnsholt
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D001996:Bronchopneumonia / Q000382:microbiology*; D002908:Chronic Disease; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000150:complications*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S