Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning.
We report on label-free monitoring of microfluidic free-flow electrophoresis (μFFE) separations in real-time using a custom built high speed deep UV laser scanner. In combination with a novel layout realized in fused silica (FS) FFE chips the setup was successfully applied for continuous separations and detection of unlabeled analytes including native proteins by space-resolved intrinsic deep UV fluorescence scanning.
Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, 04103 Leipzig, Germany.
Stefan
S
Köhler
Stefan
S
Nagl
Stefanie
S
Fritzsche
Detlev
D
Belder
D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D007834:Lasers; D011506:Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014466:Ultraviolet Rays
D011506:Proteins