Newborn neurons in the olfactory bulb selected for long-term survival through olfactory learning are prematurely suppressed when the olfactory memory is erased.
A role for newborn neurons in olfactory memory has been proposed based on learning-dependent modulation of olfactory bulb neurogenesis in adults. We hypothesized that if newborn neurons support memory, then they should be suppressed by memory erasure. Using an ecological approach in mice, we showed that behaviorally breaking a previously learned odor-reward association prematurely suppressed newborn neurons selected to survive during initial learning. Furthermore, intrabulbar infusions of the caspase pan-inhibitor ZVAD (benzyloxycarbonyl-Val-Ala-Asp) during the behavioral odor-reward extinction prevented newborn neurons death and erasure of the odor-reward association. Newborn neurons thus contribute to the bulbar network plasticity underlying long-term memory.
Institut National de la Santé et de la Recherche Médicale (INSERM) 1028, CNRS 5292, Lyon Neuroscience Research Center, Neuroplasticity and Neuropathology of Olfactory Perception Team, Université Lyon1, Villeurbanne, F-Université de Lyon, F-69007 Lyon, France.
Sébastien
S
Sultan
Nolwen
N
Rey
Joelle
J
Sacquet
Nathalie
N
Mandairon
Anne
A
Didier
D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D003213:Conditioning, Psychological / Q000502:physiology*; D003463:Cues; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055495:Neurogenesis / Q000502:physiology* / Q000528:radiation effects; D009474:Neurons / Q000502:physiology*; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology* / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010775:Photic Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D012153:Retention, Psychology / Q000187:drug effects / Q000502:physiology; D012201:Reward; D012903:Smell / Q000502:physiology* / Q000528:radiation effects; D013997:Time Factors
D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D004791:Enzyme Inhibitors; D009842:Oligopeptides; D016760:Proto-Oncogene Proteins c-fos; C517629:benzyloxycarbonyl-valyl-alanyl-aspartic acid; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine