Unusual chromatographic enantioseparation behavior of naproxen on an immobilized polysaccharide-based chiral stationary phase.
Enantioseparation of naproxen was performed on an immobilized polysaccharide-based chiral stationary phase (CSP), Chiralpak IA, in the normal-phase mode. The effects of polar alcohol modifier in mobile phase and column temperature on retention, enantioseparation, and elution order were investigated. Two unusual phenomena were observed. One was solvent-induced reversal of elution order for the two enantiomers. Not only the type but also the content of polar alcohol modifier could induce the reversal. Another uncommon phenomenon was peak deformation under some chromatographic conditions.
Department of Chemistry and Environmental Science, Zhangzhou Normal University, Zhangzhou, China.
Cui
C
Xiang
Guoqing
G
Liu
Shanshan
S
Kang
Xueping
X
Guo
Bixia
B
Yao
Wen
W
Weng
Qingle
Q
Zeng
D019840:2-Propanol / Q000737:chemistry; D019342:Acetic Acid / Q000737:chemistry; D000688:Amylose / Q000031:analogs & derivatives / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D009288:Naproxen / Q000737:chemistry / Q000302:isolation & purification*; D048448:Phenylcarbamates / Q000737:chemistry*; D013237:Stereoisomerism
D048448:Phenylcarbamates; D009288:Naproxen; D000688:Amylose; D019840:2-Propanol; D019342:Acetic Acid