Polyamide recognition-mass spectrometry for distinguishing hairpin DNA from coil DNA.
The discrimination between hairpin DNA and coil DNA has been well achieved through polyamides as probes by electrospray ionization (ESI) mass spectrometry. ESI mass spectra showed that polyamides bind to hairpin DNA with high selectivity, and almost no binding with coil DNA. In addition, the noncovalent interaction between polyamides and hairpin DNA was also studied; the results show that hairpin DNA with longer stem and polyamides with more heterocycles have higher binding affinity and stability in gas phase.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Bo
B
Zheng
Yiquan
Y
Liu
Gu
G
Yuan
D001665:Binding Sites; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D055029:Inverted Repeat Sequences; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009757:Nylons / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry
D009757:Nylons; D009841:Oligonucleotides; D004247:DNA