Identification of four isoforms of aldolase B down-regulated in hepatocellular carcinoma tissues by means of two-dimensional Western blotting.
BACKGROUND
Hepatocellular carcinoma (HCC) is one of the most lethal diseases, and one of the major causes of death in Japan. Our previous research of proteomics for cancerous and paired non-cancerous tissues from patients with HCC with hepatitis C virus infection (HCV-HCC) by means of the combination of two-dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) reported that four of numerous spots of weaker intensity in cancerous tissues than in paired non-cancerous tissues were identified as four isoforms of liver type aldolase (aldolase B). In the present study, two-dimensional (2-D) Western blot analysis demonstrated a significantly lower expression of four isoforms of aldolase B in cancerous than in non-cancerous tissues.


CONCLUSIONS
Our finding of differences of expression aldolase B isoforms between cancerous and paired non-cancerous tissues for HCV-HCC may be useful for shedding light on some behaviors of aldolase B during hepatocellular carcinogenesis.
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan.
Yufeng
Y
Wang
Yasuhiro
Y
Kuramitsu
Motonari
M
Takashima
Yuichiro
Y
Yokoyama
Norio
N
Iizuka
Takao
T
Tamesa
Isao
I
Sakaida
Masaaki
M
Oka
Kazuyuki
K
Nakamura
D015153:Blotting, Western / Q000379:methods*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D002853:Chromatography, Liquid; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D053719:Tandem Mass Spectrometry
D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase