Growth factors stimulate expression of neuronal and glial miR-132.
Brain-specific microRNAs (miRs) and brain-derived neurotrophic factor (BDNF) are both involved in synaptic function. We previously reported that upregulation of miR-132 is involved in BDNF-increased synaptic proteins, including glutamate receptors (NR2A, NR2B, and GluR1) in mature cortical neurons [7]. However, the potential role of other growth factors in miR-132 induction has not been clarified. Here, we examined the effect of growth factors including basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), glial cell line-derived neurotrophic factor (GDNF), and epidermal growth factor (EGF), on expression of miR-132 and glutamate receptors in immature cortical neurons. We found that BDNF and bFGF upregulated levels of miR-132 in cortical cultures, though bFGF failed to increase glutamate receptors such as NR2A, NR2B, and GluR1. IGF-1, GDNF, and EGF did not have a positive influence on miR-132 and glutamate receptors in neuronal cultures. Furthermore, bFGF significantly upregulated miR-132 in cultured astroglial cells, while other growth factors failed to elicit such a response. It is possible that the growth factor-stimulated neuronal and glial action of miR-132 plays a critical role in brain function.
Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. numakawa@ncnp.go.jp
Tadahiro
T
Numakawa
Noriko
N
Yamamoto
Shuichi
S
Chiba
Misty
M
Richards
Yoshiko
Y
Ooshima
Soichiro
S
Kishi
Kazuo
K
Hashido
Naoki
N
Adachi
Hiroshi
H
Kunugi
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002070:Butadienes / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D009570:Nitriles / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
D002070:Butadienes; D004791:Enzyme Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; C548821:MIRN132 microRNA, rat; D035683:MicroRNAs; D009570:Nitriles; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; C113580:U 0126; D020929:Mitogen-Activated Protein Kinase Kinases