High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type".
In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.
Cork Institute of Technology, Cork, Ireland.
Pierre E
PE
Douarre
William
W
Cashman
Jim
J
Buckley
Aidan
A
Coffey
Jim M
JM
O'Mahony
D000818:Animals; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005838:Genotype; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011110:Polymorphism, Genetic; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D020080:Tandem Repeat Sequences