Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease.
OBJECTIVE
The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat.


METHODS
Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups.


RESULTS
With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model.


CONCLUSIONS
The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
School of Natural Sciences Exercise, Edith Cowan University, Australia.
Hayley
H
Abbiss
Garth L
GL
Maker
Joel
J
Gummer
Matthew J
MJ
Sharman
Jacqueline K
JK
Phillips
Mary
M
Boyce
Robert D
RD
Trengove
D000481:Allantoin / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000652:urine; D004195:Disease Models, Animal; D008401:Gas Chromatography-Mass Spectrometry; D006626:Hippurates / Q000652:urine; D007656:Ketoglutaric Acids / Q000652:urine; D008297:Male; D055432:Metabolomics / Q000379:methods; D010641:Phenotype; D007690:Polycystic Kidney Diseases / Q000652:urine*; D025341:Principal Component Analysis; D051381:Rats; D011917:Rats, Inbred Lew; D014527:Uric Acid / Q000652:urine; D016482:Urinalysis
D015415:Biomarkers; D006626:Hippurates; D007656:Ketoglutaric Acids; D014527:Uric Acid; D000481:Allantoin; C030514:hippuric acid