Optimization of automated matrix deposition for biomolecular mapping using a spotter.
Imaging mass spectrometry using matrix-assisted laser desorption/ionization allows the detailed mapping of biomolecules directly from tissue. Matrix deposition is the key step for successful imaging. The appropriate concentration and deposition of matrix is critical for extraction, desorption, and ionization of molecules from tissue without losing molecular localization. The main challenge to meet these criteria is to deposit matrix droplets homogeneously on the tissue section. This work shows how a chemical inkjet printer was used for this purpose resulting in the imaging of phosphatidylcholines and sulfatides. The intricacies involved in effective matrix deposition are discussed.
Cellular Neurobiology, NIDA IRP, National Institutes of Health, Baltimore, MD, USA.
Alice M
AM
Delvolve
Amina S
AS
Woods
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D004867:Equipment Design; D010713:Phosphatidylcholines / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*; D013433:Sulfoglycosphingolipids / Q000032:analysis*
D010713:Phosphatidylcholines; D013433:Sulfoglycosphingolipids