[Quantitative method for simultaneous assay of four coumarins with one marker in Fraxini Cortex].
OBJECTIVE
To establish a new quantitative method for simultaneous determination of multi-coumarins in Fraxini Cortex by using one chemical reference substance, and validate its feasibilities.


METHODS
The new quality evaluation method, quantitative analysis of multi-components by singer-marker (QAMS), was established and validated with Fraxini Cortex. Four main coumarins were selected as analytes to evaluate the quality and their relative correlation factors (RCF) were determined by HPLC-DAD. Within the linear range, the values of RCF at 340 nm of aesculin to asculetin, fraxin and fraxetin were 1.771, 0.799, 1.409, respectively. And the contents of aesculin in samples of Fraxini Cortex were authentically determined by the external standard method, and the contents of the three other coumarins were calculated by their RCF. The contents of these four coumarins in all samples were also determined by the external standard method.


RESULTS
Within a certain range, the RCF had a good reproducibility (RSD 2.5%-3.9%). Significant differences were not observed between the quantitative results of two methods.


CONCLUSIONS
It is feasible and suitable to evaluate the quality of Fraxini Cortex and its Yinpian by QAMS.
Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China. weihong_bj@126.com
Weihong
W
Feng
Zhimin
Z
Wang
Qiwei
Q
Zhang
Limei
L
Liu
Jinyu
J
Wang
Fei
F
Yang
D031319:Aesculus; D000925:Anticoagulants / Q000032:analysis*; D015415:Biomarkers; D003374:Coumarins / Q000032:analysis*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D004929:Esculin / Q000032:analysis; D005240:Feasibility Studies; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
D000925:Anticoagulants; D015415:Biomarkers; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C584931:asculetin; D004929:Esculin; C517354:horse chestnut seed; C105671:fraxetin; C080614:fraxin