Comparison between the effects of platelet-rich plasma and bone marrow concentrate on defect consolidation in the rabbit tibia.
OBJECTIVE
To perform a comparative analysis of the effects of platelet-rich plasma and centrifuged bone marrow aspirate on the induction of bone healing in rabbits.


METHODS
Twenty adult, male New Zealand rabbits were randomly separated into two equal groups, and surgery was performed to create a bone defect (a cortical orifice 3.3 mm in diameter) in the proximal metaphysis of each rabbit's right tibia. In the first group, platelet-rich plasma was implanted in combination with β-tricalcium phosphate (platelet-rich plasma group), and in the second group, centrifuged bone marrow in combination with β-tricalcium phosphate (centrifuged bone marrow group) was implanted. After a period of four weeks, the animals were euthanized, and the tibias were evaluated using digital radiography, computed tomography, and histomorphometry.


RESULTS
Seven samples from each group were evaluated. The radiographic evaluation confirmed the absence of fractures in the postoperative limb and identified whether bone consolidation had occurred. The tomographic evaluation revealed a greater amount of consolidation and the formation of a greater cortical bone thickness in the platelet-rich plasma group. The histomorphometry revealed a greater bone density in the platelet-rich plasma group compared with the centrifuged bone marrow group.


CONCLUSIONS
After four weeks, the platelet-rich plasma promoted a greater amount of bone consolidation than the bone marrow aspirate concentrate.
Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil. marcorto@sercomtel.com.br
Marco Antonio
MA
Batista
Tomaz Puga
TP
Leivas
Consuelo Junqueira
CJ
Rodrigues
Géssica Cantadori Funes
GC
Arenas
Donizeti Rodrigues
DR
Belitardo
Roberto
R
Guarniero
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D015519:Bone Density; D001854:Bone Marrow Cells / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000627:therapeutic use; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011817:Rabbits; D011897:Random Allocation; D013977:Tibia / Q000000981:diagnostic imaging / Q000293:injuries*; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate