Flow cytometry in clinical pathology.
Flow cytometry has had an impact upon all areas of clinical pathology and now, in the 21st century, it is truly coming of age. This study reviews the application of flow cytometry within clinical pathology with an emphasis upon haematology and immunology. The basic principles of flow cytometry are discussed, including the principles and considerations of the flow-cell and hydrodynamic focusing, detector layout and function, use of fluorochromes and multicolour flow cytometry (spectral overlap and colour compensation), alongside the strategies available for sample preparation, data acquisition and analysis, reporting of results, internal quality control, external quality assessment and flow sorting. The practice of flow cytometry is discussed, including the principles and pitfalls associated with leukocyte immunophenotyping for leukaemia and lymphoma diagnosis, immune deficiency, predicting and monitoring response to monoclonal antibody therapy, rare event detection and screening for genetic disease. Each section is illustrated with a case study. Future directions are also discussed.
Department of Immunology and Immunogenetics, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB. paul.virgo@nbt.nhs.uk
Paul F
PF
Virgo
Graham J
GJ
Gibbs
D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007938:Leukemia / Q000175:diagnosis*; D007962:Leukocytes; D008223:Lymphoma / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D010338:Pathology, Clinical; D011786:Quality Control; D012015:Reference Standards
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes