Isolation of an endosulfan-degrading bacterium from a coffee farm soil: persistence and inhibitory effect on its biological functions.
Endosulfan is a lypophilic persistent organic pollutant (POP) that has caused widespread concern due to its persistence in the environment, toxicity and bioaccumulation in living organisms. The aim of this study is to isolate endosulfan-degrading bacteria taken from five coffee farms historically exposed to this insecticide which could be used in future remediation strategies. The biodegradation capability of the isolated strain as well as endosulfan's impact on some of the strain's biological functions was studied. Endosulfan and its metabolites were analyzed using TLC and GC-MS. The isolated strain, capable of growing in a liquid culture treated with this insecticide as the sole sulfur source rather than a carbon source, was selected for further study. The isolated bacterium is Gram-negative, having the morphological and biochemical characteristics of Azotobacter sp. The remaining concentrations after 6 days, using 2 and 10 mg l(-1) of endosulfan, were 57.6 and 72.3% respectively, and the degradation constants were 0.12 d(-1) and 0.26 d(-1). Four metabolites were detected, one of which was identified as endosulfan ether. Endosulfan reduced nitrogenase activity but had no impact on indole 3-acetic acid production. Thus, these results suggest that this strain has the potential to act as a biocatalyst in endosulfan degradation.
Department of Enviromental Protection, Estación Experimental del Zaidín (EEZ-CSIC), C/ Profesor Albareda 1, 18008-Granada, Spain. jeanmanuel.castillo@eez.csic.es
Jean Manuel
JM
Castillo
Jaime
J
Casas
Esperanza
E
Romero
D001395:Azotobacter / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002855:Chromatography, Thin Layer; D003069:Coffee / Q000378:metabolism; D003105:Colombia; D004726:Endosulfan / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007210:Indoleacetic Acids / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D009591:Nitrogenase / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D012988:Soil Microbiology
D003069:Coffee; D007210:Indoleacetic Acids; D007306:Insecticides; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D009591:Nitrogenase; D004726:Endosulfan