Development and validation of a stability indicating HPLC method for simultaneous determination of four novel fluoroquinolone dimers as potential antibacterial agents.
A series of novel 6-fluoro1,4-dihydro-4-oxo-3-quinoline carboxylic acid dimers were synthesized as potential antibacterial agents from commercially available substituted fluorobenzoic acids. A stability indicating HPLC method was developed to determine these novel fluoroquinolone dimers using a systematic method development approach. Samples were subjected to stress conditions of hydrolysis, oxidation, photolysis and thermal degradation; and analyzed to demonstrate the specificity and stability indicating ability of the developed method. The precision for all four fluoroquinolone dimers was within 2.0% RSD. Calibration curves were linear (LOQ, 150%), with regression coefficients >0.99 for all dimers. The method was conveniently applied for determining purity and assay of these four novel fluoroquinolone dimers.
Analytical Development, Aptuit Laurus Private Limited, ICICI Knowledge Park, Turkapally, Shameerpet, Hyderabad, Andhra Pradesh 500078, India. muzaffarkhan76@rediffmail.com
Muzaffar
M
Khan
C Naveen Kumar
CN
Reddy
G
G
Ravindra
K V S R Krishna
KV
Reddy
P K
PK
Dubey
D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D019281:Dimerization; D004355:Drug Stability; D024841:Fluoroquinolones / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D057230:Limit of Detection; D016014:Linear Models; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D012996:Solutions