Effects of a new hemostatic agent Ankaferd Blood Stopper(®)on the intraocular tissues in rat model.
OBJECTIVE
To investigate the histopathological changes due to administration of Ankaferd Blood Stopper(®) (ABS) into intraocular tissues by an anterior chamber and intravitreal injections.


METHODS
Twenty Wistar albino rats were divided into four equal groups. Group 1 was injected 0.01 mL ABS into anterior chamber. Group 2 was injected intravitreal 0.02 mL ABS. Groups 3 and 4, which were used as controls, were injected into the anterior chamber and intravitreal 0.01 mL and 0.02 mL balanced salt solution (BSS), respectively. At 2, 5, 10, 15 and 20 days after injection, the eyes were examined under an operating microscope and were subsequently enucleated for histopathological examination.


RESULTS
Ophthalmic examination of the rats prior to enucleation revealed ocular complications ranging from conjunctival hyperemia to corneal perforation in group 1 and increased conjunctival hyperemia and discharge in group 2. No physical and histopathological anomalies were detected in groups 3 and 4. All eyes in group 1 showed mixed type inflammatory cell reaction, foreign-body reaction, stromal congestion, disintegration of the collagen fibers and loss of the epithelium of the posterior wall in the iris and ciliary body were observed histopathologically. All eyes in group 2 showed disintegration and separation of the retina, brown pigment accumulation and mixed type inflammatory cell reaction.


CONCLUSIONS
Our results indicate that the commercially available form of ABS solution exerts a toxic effect on intraocular tissues. We consider that the intraocular use of different concentrations, rather than multiple time point of ABS should be investigated.
Department of Ophthalmology, Zonguldak Karaelmas University, Göz Hastaliklari Anabilim Dali, Kozlu 67600, Zonguldak, Turkey. atillaalpay@hotmail.com
Atilla
A
Alpay
Sibel
S
Bektas
Aysegul
A
Alpay
Silay Canturk
SC
Ugurbas
Cenk
C
Evren
Suat Hayri
SH
Ugurbas
D000818:Animals; D000867:Anterior Chamber; D005123:Eye / Q000187:drug effects* / Q000473:pathology; D006490:Hemostatics / Q000008:administration & dosage / Q000009:adverse effects*; D056965:Injections, Intraocular; D008297:Male; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000008:administration & dosage / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D014822:Vitreous Body
D006490:Hemostatics; D010936:Plant Extracts; C539751:ankaferd blood stopper