Unprecedented ultra-high-resolution hydroxy group (1)H NMR spectroscopic analysis of plant extracts.
A general method is demonstrated for obtaining ultra-high resolution in the phenolic hydroxy group 1H NMR spectroscopic region, in DMSO-d6 solution, with the addition of picric acid. Line-width reduction by a factor of over 100 was observed, which resulted in line-widths ranging from 1.6 to 0.6 Hz. This unprecedented resolution, in combination with the shielding sensitivity of the hydroxy group absorptions to substituent effects at least up to 11 bonds distant and the application of 2D 1H-13C HMBC techniques, allows the unequivocal structure analysis of natural products with phenolic hydroxy groups in complex plant extracts.
Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, GR-45110, Greece.
Pantelis
P
Charisiadis
Alexandra
A
Primikyri
Vassiliki
V
Exarchou
Andreas
A
Tzakos
Ioannis P
IP
Gerothanassis
D006115:Greece; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C005858:picric acid