Identification of residues in chromodomain helicase DNA-binding protein 1 (Chd1) required for coupling ATP hydrolysis to nucleosome sliding.
Chromatin remodelers are ATP-dependent machines responsible for directionally shifting nucleosomes along DNA. We are interested in defining which elements of the chromodomain helicase DNA-binding protein 1 (Chd1) remodeler are necessary and sufficient for sliding nucleosomes. This work focuses on the polypeptide segment that joins the ATPase motor to the C-terminal DNA-binding domain. We identify amino acid positions outside the ATPase motor that, when altered, dramatically reduce nucleosome sliding ability and yet have only ∼3-fold reduction in ATPase stimulation by nucleosomes. These residues therefore appear to play a role in functionally coupling ATP hydrolysis to nucleosome sliding, and suggest that the ATPase motor requires cooperation with external elements to slide DNA past the histone core.
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218-2685.
Ashok
A
Patel
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218-2685.
Jeffrey N
JN
McKnight
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218-2685.
Pavol
P
Genzor
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218-2685. Electronic address: gdbowman@jhu.edu.
Gregory D
GD
Bowman
D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000595:Amino Acid Sequence; D002843:Chromatin / Q000737:chemistry; D002942:Circular Dichroism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D015966:Gene Expression Regulation, Fungal; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
C489847:CHD1 protein, S cerevisiae; D002843:Chromatin; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases