[Enriching blood effect comparison in three kinds of blood deficiency model after oral administration of drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb].
OBJECTIVE
Through establishing different blood deficiency animal model, to evaluate enriching blood effect changes of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb, and to explore the effect characteristics of their compatibility.


METHODS
Three different methods of acetyl phenylhydrazine (APH) hemolytic method, cyclophosphamide (CTX) chemical damage method, APH-CTX complex method were used respectively to copy different blood deficiency model mice. Changes of orbit blood routine, thymus index, spleen index and ATPase activity of red cell membrane of model mice were tested.


RESULTS
Compared with normal group, all indexes had significant differences in three model mice. The drug pair and each single herb had significant impact on most indexes of the APH-CTX complex model mice, and on the individual indexes of APH hemolytic model mice and CTX chemical damage model mice. Therefore, APH and CTX complex blood deficiency model was more suitable for the enriching blood mechanism study of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma. Compared with the single herb of Angelicae Sinensis Radix and Chuanxiong Rhizoma, the drug pair of them had presented enriching blood effect at different extent with strengthening trend in regulating the invigorating blood indexes, immune organs and energy metabolic enzymes.


CONCLUSIONS
The results of this research have provided scientific basis for revealing the mutual promotive composition law of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma, and responded effectively the mult-link and mult-target effect characteristics of Chinese medicine bio-effect, to offer reference for the bio-effect research of the complicated substance group of Chinese medicine and traditional Chinese medicine formulae, and to supply demonstrative reference for researching the formulae compatibility law which takes the single drug-drug pair-formulae as main line.
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China. liweixia01@126.com
Weixia
W
Li
Yuping
Y
Tang
Jianming
J
Guo
Meiyan
M
Huang
Wei
W
Li
Dawei
D
Qian
Jin'ao
J
Duan
D000284:Administration, Oral; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000187:drug effects / Q000378:metabolism; D003520:Cyclophosphamide / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D005260:Female; D006402:Hematologic Diseases / Q000188:drug therapy / Q000209:etiology; D006454:Hemoglobins / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects; D030004:Ligusticum; D008516:Medicine, Chinese Traditional; D051379:Mice; D023421:Models, Animal; D010659:Phenylhydrazines / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011897:Random Allocation; D027343:Rhizome / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
D004365:Drugs, Chinese Herbal; D006454:Hemoglobins; D010659:Phenylhydrazines; C030299:phenylhydrazine; D003520:Cyclophosphamide; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; C101574:chuangxiong extract