NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
David A
DA
Elliott
Stefan R
SR
Braam
Katerina
K
Koutsis
Elizabeth S
ES
Ng
Robert
R
Jenny
Ebba L
EL
Lagerqvist
Christine
C
Biben
Tanya
T
Hatzistavrou
Claire E
CE
Hirst
Qing C
QC
Yu
Rhys J P
RJ
Skelton
Dorien
D
Ward-van Oostwaard
Sue Mei
SM
Lim
Ouda
O
Khammy
Xueling
X
Li
Susan M
SM
Hawes
Richard P
RP
Davis
Adam L
AL
Goulburn
Robert
R
Passier
Owen W J
OW
Prall
John M
JM
Haynes
Colin W
CW
Pouton
David M
DM
Kaye
Christine L
CL
Mummery
Andrew G
AG
Elefanty
Edouard G
EG
Stanley
D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D032386:Myoblasts, Cardiac / Q000166:cytology* / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
D000943:Antigens, Differentiation; D015415:Biomarkers; D000072576:Homeobox Protein Nkx-2.5; D018398:Homeodomain Proteins; C095552:NKX2-5 protein, human; D011971:Receptors, Immunologic; C501920:SIRPA protein, human; D014157:Transcription Factors; D019010:Vascular Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins