Melatonin modulates wireless (2.45 GHz)-induced oxidative injury through TRPM2 and voltage gated Ca(2+) channels in brain and dorsal root ganglion in rat.
We aimed to investigate the protective effects of melatonin and 2.45 GHz electromagnetic radiation (EMR) on brain and dorsal root ganglion (DRG) neuron antioxidant redox system, Ca(2+) influx, cell viability and electroencephalography (EEG) records in the rat. Thirty two rats were equally divided into four different groups namely group A1: Cage control, group A2: Sham control, group B: 2.45 GHz EMR, group C: 2.45 GHz EMR+melatonin. Groups B and C were exposed to 2.45 GHz EMR during 60 min/day for 30 days. End of the experiments, EEG records and the brain cortex and DRG samples were taken. Lipid peroxidation (LP), cell viability and cytosolic Ca(2+) values in DRG neurons were higher in group B than in groups A1 and A2 although their concentrations were increased by melatonin, 2-aminoethyldiphenyl borinate (2-APB), diltiazem and verapamil supplementation. Spike numbers of EEG records in group C were lower than in group B. Brain cortex vitamin E concentration was higher in group C than in group B. In conclusion, Melatonin supplementation in DRG neurons and brain seems to have protective effects on the 2.45 GHz-induced increase Ca(2+) influx, EEG records and cell viability of the hormone through TRPM2 and voltage gated Ca(2+) channels.
Department of Biophysics, Medicine Faculty, Süleyman Demirel University, Isparta, Turkey. mnaziroglu@med.sdu.edu.tr
M
M
Nazıroğlu
Ö
Ö
Çelik
C
C
Özgül
B
B
Çiğ
S
S
Doğan
R
R
Bal
N
N
Gümral
A B
AB
Rodríguez
J A
JA
Pariente
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism*; D004569:Electroencephalography / Q000379:methods; D060733:Electromagnetic Radiation; D005727:Ganglia, Spinal / Q000166:cytology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D008550:Melatonin / Q000627:therapeutic use*; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D050053:TRPM Cation Channels / Q000378:metabolism*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D050053:TRPM Cation Channels; C557080:Trpm2 protein, rat; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D005978:Glutathione; D008550:Melatonin; D002118:Calcium