Metabolic engineering to improve 5-aminolevulinic acid production.
5-Aminolevulinic acid (ALA) has recently attracted significant attentions due to its potential applications in many diverse fields. The majority of engineered ALA producers are based on the whole cell catalysis, supplemented with succinate and glycine as precursors. Recently, we succeeded in producing ALA directly from inexpensive glucose, through re-constructing the native C5 pathway of ALA synthesis in Escherichia coli. Herein, we further discuss ALA production by manipulating the C5 and C4 pathways in Escherichia coli through the strategy of metabolic engineering.
State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Zhen
Z
Kang
Yang
Y
Wang
Qiang
Q
Wang
Qingsheng
Q
Qi
D000624:5-Aminolevulinate Synthetase / Q000235:genetics* / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005947:Glucose / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D010957:Plasmids; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics*; D019802:Succinic Acid / Q000378:metabolism; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D000622:Aminolevulinic Acid; D019802:Succinic Acid; D000624:5-Aminolevulinate Synthetase; D005947:Glucose; D005998:Glycine