[Expression and pharmacological evaluation of fusion protein FGF21-L-Fc].
FGF21 (fibroblast growth factor 21) is a recently described member of the FGF family. It has been previously demonstrated that FGF21 is a potent regulator of glucose homeostasis. To improve stability of FGF21 for better efficacy, a new form of recombinant FGF21 was generated by fusion of a full length FGF21 gene and the Fc fragment of human IgG4 with flexible linker sequence. To examine the glucose regulation activity of FGF21-L-Fc, 3T3-L1 pre-adipocytes were differentiated into adipocytes, and glucose uptake activity of FGF21-L-Fc was examined by glucose oxidase and peroxidase (GOD-POD) assay. The results showed that in comparison with wild type FGF21, FGF21-L-Fc was more potent in stimulation of glucose uptake by 3T3-L1. In vivo studies on the modified protein demonstrated that FGF-L-Fc had a better efficacy in lowering blood glucose of the STZ-induced diabetic animals and controlled glucose level for a longer time. The results provided a sound basis for further studies.
College of Life Science of Northeast Agricultural University, Harbin 150030, China.
Wen-Bing
WB
Yao
Gui-Ping
GP
Ren
Yang
Y
Han
Hong-Wei
HW
Cao
Hong-Mei
HM
Gao
Fang-Ming
FM
Kan
Qi
Q
Wang
De-Shan
DS
Li
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007074:Immunoglobulin G / Q000235:genetics / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology
D001786:Blood Glucose; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D005947:Glucose