Shotguns in the front line: phosphoproteomics in plants.
The emergence of 'shotgun proteomics' has paved the way for high-throughput proteome analysis, by which thousands of proteins can be identified simultaneously from complex samples. Although the shotgun approach has the potential to monitor many different post-translational modifications, further technological development is needed to enrich each post-translational 'modificome'. Large-scale in vivo phosphorylation site mapping, so-called shotgun phosphoproteomics, has become feasible in various organisms, including plants, owing to recent technological breakthroughs. Shotgun phosphoproteomics is not a mature technology, but progress has been rapid. In this review, we highlight the scope and limitations of current methods, and some key technological issues in this field.
RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan. hironakagami@psc.riken.jp
Hirofumi
H
Nakagami
Naoyuki
N
Sugiyama
Yasushi
Y
Ishihama
Ken
K
Shirasu
D010748:Phosphopeptides / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D010748:Phosphopeptides; D010750:Phosphoproteins; D010940:Plant Proteins