Evaluation of the protective potential of a Taenia solium cysticercus mimotope on murine cysticercosis.
An NC-1 mimotope from Taenia solium cysticerci can help identify patients with neurocysticercosis through immunoassay. After chemical synthesis, an NC-1 peptide was coupled to bovine serum albumin (NC-1/BSA) for used as an immunogen in murine Taenia crassiceps cysticercosis, which is an experimental model of cysticercosis caused by T. solium. NC-1/BSA immunisation decreased parasitaemia by inducing 74% protection compared to the 77% protection obtained with T. crassiceps crude antigen. The influence of immunisation was also observed on the size and stage of development of the parasite. Antibodies from NC-1/BSA-immunised mice recognised proteins from the tegument and from the buddings, and intense immunostaining was observed in the final stage of the metacestode. The capacity of NC-1/BSA to induce protective antibodies which are reactive to proteins from the tegument of the metacestode suggests that this mimotope is a potential candidate for a vaccine against human and animal cysticercosis.
Departamento de Patologia Básica, UFPR, Curitiba - PR, Brazil.
Janaína
J
Capelli-Peixoto
Carlos
C
Chávez-Olórtegui
Daniele
D
Chaves-Moreira
João Carlos
JC
Minozzo
Juarez
J
Gabardo
Kádima Nayara
KN
Teixeira
Vanete
V
Thomaz-Soccol
Larissa Magalhães
LM
Alvarenga
Juliana
J
de Moura
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis / Q000276:immunology* / Q000517:prevention & control; D003552:Cysticercus / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D007814:Larva / Q000276:immunology; D051379:Mice; D012710:Serum Albumin, Bovine; D041201:Taenia solium / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D014612:Vaccines; D012710:Serum Albumin, Bovine