HSP27 and HSP70 serum and urine levels in patients suffering from chronic kidney disease.
BACKGROUND
Chronic kidney disease (CKD) is a condition associated with inflammation and high levels of uremic toxins and reactive oxygen species. As a counterregulation to systemic stress heat shock proteins (HSP) are increased expressed to minimize cell death and preserve cell integrity by inhibiting apoptotic pathways. The aim of this study was to determine HSP27 and HSP70 concentrations in sera and urine of patients suffering from CKD.


METHODS
Concentrations of HSP27 and HSP70 in urine and serum were determined in 119 patients with CKD stages 1 to 5 and 23 healthy volunteers by using ELISA technique.


RESULTS
HSP27 serum levels were significantly elevated in patients suffering from CKD stages 3 to 5 as well as fractional HSP27 excretion in stages 2-5 versus healthy controls. Absolute HSP70 urinary values were significantly elevated in stages 4 and 5 and fractional HSP70 excretion was increased in stage 5 compared to controls. Moreover, ROC curve analysis showed the potential of urine and especially serum HSP levels to identify various stages of CKD.


CONCLUSIONS
We provide evidence for elevated HSP27 concentrations in serum and urine and increased HSP70 excretion levels in patients suffering from CKD. Moreover, our results show that HSP levels might offer potential to examine the stages of CKD as well as the disease course which could further promote individually adjusted treatment planning.
Department of Anesthesiology, General Intensive Care and Pain Medicine, Medical University of Vienna, Vienna, Austria.
Diana
D
Lebherz-Eichinger
Hendrik J
HJ
Ankersmit
Stefan
S
Hacker
Hubert
H
Hetz
Oliver
O
Kimberger
Elisabeth M
EM
Schmidt
Thomas
T
Reiter
Walter H
WH
Hörl
Martin
M
Haas
Claus G
CG
Krenn
Georg A
GA
Roth
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008297:Male; D008875:Middle Aged
D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins