Genistein downregulates presenilin 1 and ubiquilin 1 expression.
The aim of this study was to determine the effects of several food ingredients and chemical inhibitors on the expression of presenilin, a molecule involved in γ-secretase activity and the generation of amyloid-β peptide in Alzheimer's disease. Western blotting revealed the downregulation of presenilin 1 protein expression by stimulation with genistein in vitro, while the effects on presenilin 1 gene expression examined by reverse transcriptase-polymerase chain reaction (RT-PCR) were unaltered in Daudi cells. Genistein likely downregulates presenilin via the inhibition of ubiquilin 1 expression in lymphoid cells. Our findings provide new insights that may help to establish preventive strategies against Alzheimer's disease.
Department of Environmental Health Science, Nara Women's University,                     Nara 630-8506, Japan.
Naoko
N
Okumura
Hitomi
H
Yoshida
Yuri
Y
Nishimura
Mutsumi
M
Murakami
Yasuko
Y
Kitagishi
Satoru
S
Matsuda
D048868:Adaptor Proteins, Signal Transducing; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D053764:Presenilin-1 / Q000235:genetics / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D016588:Anticarcinogenic Agents; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D053764:Presenilin-1; C107792:UBQLN1 protein, human; D019833:Genistein