Cellulomonas carbonis sp. nov., isolated from coal mine soil.
A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain T26(T), was isolated from subsurface soil of Tianjin coal mine, China. Colonies were yellow-white, convex, circular, smooth and non-transparent on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T26(T) was closely related to members of the genus Cellulomonas and a member of the genus Actinotalea with 96.8-94.7% and 96.7% gene sequence similarities, respectively. The peptidoglycan type of strain T26(T) was A4β, containing l-ornithine-d-glutamic acid as the interpeptide bridge. The cell-wall sugars were rhamnose, galactose, xylose and inositol. The major fatty acids (>10%) were anteiso-C(15:0) (33.6%), anteiso-C(15:1) A (22.1%), C(16:0) (14.4%) and C(14:0) (12.1%). The predominant respiratory quinone was MK-9(H(4)) and the genomic DNA G+C content was 74.4 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol-mannosides and phosphatidylinositol. Comparison of phenotypic and phylogenetic characteristics between strain T26(T) and related organisms revealed that the new isolate represented a novel species of the genus Cellulomonas, for which the name Cellulomonas carbonis sp. nov. is proposed. The type strain is T26(T) ( = CGMCC 1.10786(T) = KCTC 19824(T) = CCTCC AB2010450(T)).
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Zunji
Z
Shi
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Guosheng
G
Luo
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Gejiao
G
Wang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D040143:Cellulomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D003032:Coal Mining; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012987:Soil