[Advances in algae tool enzymes: alginate lyases].
Marine can be considered as a rather unexplored source of biological material. Production of algal oligosaccharides by using valuable enzymes from marine origin has become an important way to utilize marine resources. As one of algal tool enzymes, the use of alginate lyases has been focused mainly on development and application of alginate oligosaccharides with bioactive function in recent years. In this paper, we reviewed the research of alginate lyases over the past decade in several aspects, including their origin, diversity, substrate specification, mode of action, structure and catalysis mechanism, assay of enzyme activity, enzyme characterization, as well as our own experience on this subject. At the end of the review, the application prospects of alginate lyases are presented.
College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China.
Liyan
L
Li
Huashi
H
Guan
Xiaolu
X
Jiang
Jianjun
J
Hao
D000464:Alginates / Q000378:metabolism*; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D008386:Marine Biology / Q000379:methods*; D009844:Oligosaccharides / Q000378:metabolism*; D000459:Phaeophyceae / Q000201:enzymology; D011133:Polysaccharide-Lyases / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase