Dietary cholecalciferol regulates the recruitment and growth of skeletal muscle fibers and the expressions of myogenic regulatory factors and the myosin heavy chain in European sea bass larvae.
The aim of this study was to determine whether dietary cholecalciferol affects the recruitment and growth of axial skeletal muscle fibers in first-feeding European sea bass. Larvae were fed diets containing 0.28 (VD-L, low dose), 0.69 (VD-C, control dose), or 3.00 (VD-H, high dose) mg cholecalciferol/kg from 9 to 44 d posthatching (dph). Larvae were sampled at 44 dph for quantification of somatic growth, muscle growth, and muscle growth dynamics and at 22 and 44 dph for the relative quantification of transcripts encoded by genes involved in myogenesis, cell proliferation, and muscle structure. The weight increase of the VD-L-fed larvae was less than that of the VD-H-fed group, whereas that of VD-C-fed larvae was intermediate. The level of expression of genes involved in cell proliferation (PCNA) and early myogenesis (Myf5) decreased between 22 and 44 dph, whereas that of the myogenic determination factor MyoD1 and that of genes involved in muscle structure and function (myosin heavy chain, myosin light chains 2 and 3) increased. Dietary cholecalciferol regulated Myf5, MyoD1, myogenin, and myosin heavy chain gene expression, with a gene-specific shape of response. The maximum hypertrophy of white muscle fibers was higher in larvae fed the VD-C and VD-H diets than in larvae fed the VD-L diet. White muscle hyperplasia was highly stimulated in VD-H-fed larvae compared to VD-L- and VD-C-fed ones. These findings demonstrate a dietary cholecalciferol effect on skeletal muscle growth mechanisms of a Teleost species.
INRA, UR 1067 Nutrition Métabolisme Aquaculture, Saint-Pée-sur-Nivelle, France. alami@st-pee.inra.fr
Hélène
H
Alami-Durante
Marianne
M
Cluzeaud
Didier
D
Bazin
David
D
Mazurais
José L
JL
Zambonino-Infante
D000818:Animals; D001492:Bass / Q000254:growth & development / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002762:Cholecalciferol / Q000008:administration & dosage*; D004032:Diet; D015870:Gene Expression; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018006:Myogenic Regulatory Factors / Q000187:drug effects / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D018006:Myogenic Regulatory Factors; D002762:Cholecalciferol; D018995:Myosin Heavy Chains