Effects of adenovirus vectors mediated human lactoferrin cDNA on mice bearing EMT6 breast carcinoma.
Human lactoferrin (hLTF) is an 80KD iron-binding protein. It has been reported that hLTF exists anti-tumor effects. In this study Adenovirus Vectors Mediated Human Lactoferrin cDNA (ad-rhLTF) was constructed and an antitumor effects of ad-rhLTF were investigated in mice bearing EMT6 breast carcinoma. The results demonstrated that ad-rhLTF (5 x 10(8) and 25 x 10(8) pfu/ml local injection) had high expression in tumor tissues and effectively reduced the weight of EMT6 breast tumors. Compared with the control group, cell cycle assay by flow cytometry showed that ad-rhLTF increased the percentage of tumor cells in the Sub-G1 phase and G0/G1 phase and the apoptotic number reached to 23.2% in ad-rhLTF group (25 x 10(8) pfu/ml). Ad-rhLTF treatment also resulted in a decrease of Bcl-2 and an increase in Bax and caspase 3 expressions, which was demonstrated by immunohistochemical analysis and RT-PCR. These data suggest that the antitumor effects of ad-rhLTF might be associated with arresting tumor cells in the G0/G1 phase, inducing cell apoptosis and regulation of the expression of Bcl-2, Bax and activation of caspase 3.
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, Qinhuangdao, PR China.
Jianjie
J
Wang
Qingwang
Q
Li
Kun
K
Li
Yetao
Y
Ou
Zengsheng
Z
Han
Dawei
D
Gao
Jian
J
Li
D000256:Adenoviridae; D000818:Animals; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000628:therapy*; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009368:Neoplasm Transplantation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
D018076:DNA, Complementary; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins; D012313:RNA; D007781:Lactoferrin; D053148:Caspase 3