Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1 in toluene-water biphasic system.
Benzoylformic acid was synthesized from the hydrolysis of benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1. In this study, an aqueous-toluene biphasic system was developed for highly efficient production of benzoylformic acid from the hydrolysis of benzoyl cyanide. In the aqueous-toluene biphasic system, the phase volume ratio, buffer pH and reaction temperature were optimized. Using fed-batch method, a total of 932 mM benzoylformic acid accumulated in the reaction mixture after the 10th feed. Moreover, enzymatic hydrolysis of benzoyl cyanide using calcium alginate entrapped resting cells was carried out in the aqueous-toluene biphasic system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 14.26g benzoylformic acid g(-1) dry cell weight (DCW) cell after 20 cycles of repeated use.
Laboratory of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou 213164, China. yucaihe2007@yahoo.com.cn
Yu-Cai
YC
He
Qiong
Q
Zhou
Cui-Luan
CL
Ma
Zhi-Qiang
ZQ
Cai
Li-Qun
LQ
Wang
Xi-Yue
XY
Zhao
Qun
Q
Chen
Da-Zhou
DZ
Gao
Ming
M
Zheng
Xu-Dong
XD
Wang
Quan
Q
Sun
D061249:Batch Cell Culture Techniques; D055162:Biocatalysis / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007477:Ions; D008333:Mandelic Acids / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D009570:Nitriles / Q000378:metabolism*; D044367:Phase Transition / Q000187:drug effects; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D059027:Recycling; D012240:Rhodococcus / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D014050:Toluene / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D006038:Glyoxylates; D007477:Ions; D008333:Mandelic Acids; D008670:Metals; D009570:Nitriles; D012336:RNA, Ribosomal, 16S; D012997:Solvents; D014867:Water; C012482:phenylglyoxylic acid; D014050:Toluene