Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis.
Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.
Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, CNRS-URA 3012, 25 rue du Dr Roux 75724 Paris, cedex 15, France.
Elisabeth
E
Njamkepo
Stéphane
S
Bonacorsi
Monique
M
Debruyne
Sophie Anne
SA
Gibaud
Sophie
S
Guillot
Nicole
N
Guiso
D000293:Adolescent; D000328:Adult; D001884:Bordetella / Q000235:genetics / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005602:France; D006801:Humans; D008875:Middle Aged; D009305:Nasopharynx / Q000382:microbiology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D055815:Young Adult
D004251:DNA Transposable Elements; D004269:DNA, Bacterial