Nuclear magnetic resonance-based metabolomics enable detection of the effects of a whole grain rye and rye bran diet on the metabolic profile of plasma in prostate cancer patients.
Prostate cancer (PC) is the most common cancer in the Western world and the second most important cancer causing male deaths, after lung cancer, in the United States and Britain. Lifestyle and dietary changes are recommended for men diagnosed with early-stage PC. It has been shown that a diet rich in whole grain (WG) rye reduces the progression of early-stage PC, but the underlying mechanism is not clear. This study sought to identify changes in the metabolic signature of plasma in patients with early-stage PC following intervention with a diet rich in WG rye and rye bran product (RP) compared with refined white wheat product (WP) as a tool for mechanistic investigation of the beneficial health effects of RP on PC progression. Seventeen PC patients received 485 g RP or WP in a randomized, controlled, crossover design during a period of 6 wk with a 2-wk washout period. At the end of each intervention period, plasma was collected after fasting and used for (1)H NMR-based metabolomics. Multilevel partial least squares discriminant analysis was used for paired comparisons of multivariate data. A metabolomics analysis of plasma showed an increase in 5 metabolites, including 3-hydroxybutyric acid, acetone, betaine, N,N-dimethylglycine, and dimethyl sulfone, after RP. To understand these metabolic changes, fasting plasma homocysteine, leptin, adiponectin, and glucagon were measured separately. The plasma homocysteine concentration was lower (P = 0.017) and that of leptin tended to be lower (P = 0.07) after RP intake compared to WP intake. The increase in plasma 3-hydroxybutyric acid and acetone after RP suggests a shift in energy metabolism from anabolic to catabolic status, which could explain some of the beneficial health effects of WG rye, i.e., reduction in prostate-specific antigen and reduced 24-h insulin secretion. In addition, the increase in betaine and N,N-dimethylglycine and the decrease in homocysteine show a favorable shift in homocysteine metabolism after RP intake.
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. Ali.Moazzami@lmv.slu.se
Ali A
AA
Moazzami
Jie-Xian
JX
Zhang
Afaf
A
Kamal-Eldin
Per
P
Aman
Göran
G
Hallmans
Jan-Erik
JE
Johansson
Sven-Olof
SO
Andersson
D020155:3-Hydroxybutyric Acid / Q000097:blood; D000368:Aged; D000369:Aged, 80 and over; D001622:Betaine / Q000097:blood; D018592:Cross-Over Studies; D004032:Diet; D006710:Homocysteine / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D028321:Plant Preparations / Q000494:pharmacology*; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000097:blood; D012434:Secale / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism; D014481:United States
D007328:Insulin; D028321:Plant Preparations; D006710:Homocysteine; D001622:Betaine; C025138:dimethylglycine; D017430:Prostate-Specific Antigen; D020155:3-Hydroxybutyric Acid; D012521:Sarcosine