RNA interference mediated YAP gene silencing inhibits invasion and metastasis of human gastric cancer cell line SGC-7901.
OBJECTIVE
This study aimed to investigate the effects of yes-associated protein (YAP) silencing on invasion, migration, anchorage-independent growth and angiogenesis in the human gastric cancer cell line SGC-7901.


METHODS
SGC-7901 cells were transfected with a shRNA lentivirus vector targeting YAP gene. After puromycin selection of stable YAP-silenced SGC-7901 cell lines, the expression of YAP mRNA and protein were detected using quantitative real-time PCR (qRT-PCR) and western blotting. A transwell invasion assay, wound healing assay and colony formation assay were used to analyze the invasion, migration and anchorage-independent growth effect of RNA interference (RNAi) on YAP expression. The angiogenic cytokines, vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), were measured by qRT-PCR and western blotting.


RESULTS
The results of the qRT-PCR and western blotting showed an obvious inhibition of YAP mRNA and protein expression in the YAP-shRNA group. Moreover, the inhibition of YAP expression clearly impeded SGC-7901 cell migration, invasion and anchorage-independent growth. Furthermore, VEGF and FGF-2 were also downregulated.


CONCLUSIONS
YAP gene targeting by RNAi can inhibit the migration, invasion, anchorage-independent growth and angiogenesis ability of SGC-7901 cells. YAP may be a potential target for the treatment of human gastric cancer.
Department of Gastroenterology, Shanghai Jiaotong University, Shanghai, China.
Zhou
Z
Zhou
Jin-Shui
JS
Zhu
Zhi-Peng
ZP
Xu
D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000473:pathology*; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014945:Wound Healing
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D042461:Vascular Endothelial Growth Factor A; C456659:YY1AP1 protein, human; D016222:Fibroblast Growth Factor 2