Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape.
The potential toxic effects of copper oxide (CuO) nanoparticles (NPs) were studied on differentiated Caco-2 cell monolayers, a classical in vitro model of human small intestine epithelium. Two types of CuO NPs, with different specific surface area, different sizes as raw material but the same hydrodynamic diameter in suspension, differentially disturbed the monolayer integrity, were cytotoxic and triggered an increase of the abundance of several transcripts coding for pro-inflammatory cytokines and chemokines. Specific surface area was not a major variable explaining the increased toxicity when intestinal epithelium is exposed to rod-shaped CuO NPs, compared with spherical CuO NPs. The results suggest that release of Cu(II) cations and shape of these CuO NPs are likely to be implicated in the toxicity of these CuO NPs.
URBC, Namur Research Institute for Life Sciences (NARILIS), University of Namur (FUNDP), Namur, Belgium.
Jean-Pascal
JP
Piret
Sébastien
S
Vankoningsloo
Jorge
J
Mejia
Florence
F
Noël
Emmanuelle
E
Boilan
Françoise
F
Lambinon
Christos C
CC
Zouboulis
Bernard
B
Masereel
Stéphane
S
Lucas
Christelle
C
Saout
Olivier
O
Toussaint
D000704:Analysis of Variance; D018938:Caco-2 Cells; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects*; D003300:Copper / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D057446:Hydrodynamics; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D013499:Surface Properties
D016209:Interleukin-8; D003300:Copper; C030973:cupric oxide