Development of new microsatellite DNA markers from Apostichopus japonicus and their cross-species application in Parastichopus parvimensis and Pathallus mollis.
Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers.
Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China; E-Mails:  liaomj@ysfri.ac.cn  (M.L.);  rongxj@ysfri.ac.cn  (X.R.);  zhangzheng@ysfri.ac.cn  (Z.Z.);  libin841008@163.com  (B.L.);  wanglan0829@yahoo.cn  (L.W.);  chengp@ysfri.ac.cn  (G.C.).
Meijie
M
Liao
Yingeng
Y
Wang
Xiaojun
X
Rong
Zheng
Z
Zhang
Bin
B
Li
Lan
L
Wang
Guiping
G
Chen
D000818:Animals; D002681:China; D005787:Gene Frequency; D005838:Genotype; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012616:Sea Cucumbers / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D047528:Stichopus / Q000235:genetics*