Advantage of higher-avidity CTL specific for Tax against human T-lymphotropic virus-1 infected cells and tumors.
Strong CTL response can be observed and associated with the control of proviral load in human T-lymphotropic virus type 1 (HTLV-1) infection. However, there are few details with regard to how HTLV-1 specific CTLs work against HTLV-1 infected cells and adult T-cell leukemia cells (ATLs). In this study, using Tax-specific CTL lines with high- and low-functional avidity developed from HLA-A2-transgenic mice, we showed that higher avidity CTLs specific for Tax expressing larger numbers of TCRs and better binding strength to the antigen-HLA-A2 complex are much more efficient at eliminating HTLV-1 infected cells and, in particular, ATL tumor cells with the ability of recognizing a latent level of Tax product detected only with a real-time PCR. These findings suggest that such higher avidity CTLs specific for Tax in HTLV-1 could be responsible for preventing the development of HTLV-1 infection by detecting trace amount of antigens.
Division of Rheumatology and Allergy, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.
Takako
T
Kitazono
Takahiro
T
Okazaki
Natsumi
N
Araya
Yoshihisa
Y
Yamano
Yasuaki
Y
Yamada
Tatsufumi
T
Nakamura
Yuetsu
Y
Tanaka
Makoto
M
Inoue
Shoichi
S
Ozaki
D000818:Animals; D019070:Cell Lineage; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D016356:Gene Products, tax / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D015490:HTLV-I Infections / Q000150:complications / Q000276:immunology / Q000473:pathology / Q000628:therapy* / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000628:therapy* / Q000821:virology; D051379:Mice; D008822:Mice, Transgenic; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D017735:Virus Latency / Q000276:immunology
D000939:Epitopes; D016356:Gene Products, tax; D015789:HLA-A2 Antigen; D011948:Receptors, Antigen, T-Cell; C514970:tax protein, Human T-lymphotrophic virus 1