Potassium bromate causes cell lysis and induces oxidative stress in human erythrocytes.
In the present study, we have studied the effect of KBrO3 on human erythrocytes under in vitro conditions. Erythrocytes were isolated from the blood of healthy nonsmoking volunteers and incubated with different concentrations of KBrO3 at 37°C for 60 min. This resulted in marked hemolysis in a KBrO3 -concentration dependent manner. Lysates were prepared from KBrO3 -treated and control erythrocytes and assayed for various parameters. KBrO3 treatment caused significant increase in protein oxidation, lipid peroxidation, hydrogen peroxide levels, and decrease in total sulfhydryl content, which indicates induction of oxidative stress in human erythrocytes. Methemoglobin levels and methemoglobin reductase activity were significantly increased while the total antioxidant power of lysates was greatly reduced upon KBrO3 treatment. Intracellular production of reactive oxygen species increased in a dose dependent manner. Exposure of erythrocytes to KBrO3 also caused decrease in the activities of catalase, glutathione peroxidase, thioredoxin reductase, glucose 6-phosphate dehydrogenase and glutathione reductase whereas the activities of Cu-Zn superoxide dismutase and glutathione-S-transferase were increased. These results show that KBrO3 induces oxidative stress in human erythrocytes through the generation of reactive oxygen species and alters the cellular antioxidant defense system.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India.
Mir Kaisar
MK
Ahmad
Samreen
S
Amani
Riaz
R
Mahmood
D000328:Adult; D001959:Bromates / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005503:Food Additives / Q000633:toxicity*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006203:Hair Preparations; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism; D009336:Necrosis / Q000139:chemically induced; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D018508:Water Purification; D055815:Young Adult
D001959:Bromates; D002273:Carcinogens; D005503:Food Additives; D006203:Hair Preparations; D017392:Thiobarbituric Acid Reactive Substances; C019536:potassium bromate; D008706:Methemoglobin; D006861:Hydrogen Peroxide; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D013880:Thioredoxin-Disulfide Reductase; D005982:Glutathione Transferase