Modification and labeling of AAV vector particles.
Adeno-associated virus (AAV) has become a versatile vector platform. In recent years, powerful -techniques for the generation of tropism-modified vectors (rAAV-targeting vectors) and for investigation of virus-cell interaction were developed. The following chapter describes strategies for insertion of peptide ligands into the viral capsid and the subsequent characterization of capsid mutants, for producing mosaic capsids and for labeling the viral capsid chemically or genetically.
Department of Internal Medicine, University of Cologne, Cologne, Germany. hildegard.buening@uk-koeln.de
Hildegard
H
Büning
Chelsea M
CM
Bolyard
Michael
M
Hallek
Jeffrey S
JS
Bartlett
D002213:Capsid / Q000737:chemistry; D000229:Dependovirus / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014771:Virion / Q000737:chemistry