RPE and neuronal differentiation of allotransplantated porcine ciliary epithelium-derived cells.
OBJECTIVE
Cell replacement has the potential to be applied as a therapeutic strategy in retinal degenerative diseases such as retinitis pigmentosa and age-related macular degeneration (AMD) for which no adequate pharmacological and surgical treatments are currently available. Although controversial, the use of ciliary epithelium (CE)-derived cells is supported by evidence showing their differentiation into retinal phenotypes. This study examines the differentiation potential of porcine CE-derived cells in vitro and their survival, migration, morphological characteristics, and immunohistochemical phenotype in vivo, upon transplantation into the subretinal space of normal pigs.


METHODS
Cells were isolated from the CE of postnatal pigs and were grown in a suspension sphere culture. Differentiation was assessed in vitro after exposure to laminin and the addition of serum. For transplantation, CE-derived spheres were dissociated, labeled with CM-DiI vital dye, and the cells were injected subretinally into one eye of eight week-old allorecipients. The eyes were examined at eight days and at two and four weeks after transplantation.


RESULTS
Cells positive for neuronal and retinal pigment epithelium (RPE) markers were detected by immunohistochemistry in differentiation cultures. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) revealed upregulation of neuronal markers after in vitro differentiation. CM-DiI dye-labeled CE-derived cells dissociated from primary spheres survived for up to four weeks after transplantation in vivo. Some of the surviving cells migrated distantly from the injection site. Large clusters of transplanted cells integrated into the RPE layer and multilayered RPE-like structures positive for RPE65 were often observed. Grafted cells were also identified in the neuroretina where 5%-10% were positive for recoverin, protein kinase C alpha (PKCα), and calbindin.


CONCLUSIONS
The efficient conversion to an RPE-like phenotype suggests that CE-derived cells could be a potential source of RPE for cell replacement. Our data also suggest that the ability of these cells to acquire neuronal phenotypes is influenced by the environment. Thus, pre-differentiated or (re)programmed CE-derived cells may be more amenable for retinal repair.
Centre for Vision and Vascular Sciences, Queen’s University Belfast, Royal Victoria Hospital, Institute of Clinical Science, Northern Ireland, UK. j.guduricfuchs@qub.ac.uk
Jasenka
J
Guduric-Fuchs
Wing
W
Chen
Henrietta
H
Price
Desmond B
DB
Archer
Tiziana
T
Cogliati
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D002232:Carbocyanines / Q000032:analysis; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis; D007150:Immunohistochemistry; D056965:Injections, Intraocular / Q000379:methods*; D007797:Laminin / Q000494:pharmacology; D008268:Macular Degeneration / Q000473:pathology / Q000628:therapy; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000166:cytology* / Q000254:growth & development; D012174:Retinitis Pigmentosa / Q000473:pathology / Q000628:therapy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014184:Transplantation, Homologous; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase
D015415:Biomarkers; D002232:Carbocyanines; D002352:Carrier Proteins; D005136:Eye Proteins; D005456:Fluorescent Dyes; D007797:Laminin; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase; C101089:CM-DiI