Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone--application to molecularly imprinted sorbent assays.
Biomimetic testosterone receptors were synthesized via molecular imprinting for use as antibody mimics in immunoassays. As evaluated by radioligand binding assays, imprinted polymers prepared in acetonitrile were very specific for testosterone because the nonimprinted control polymers bound virtually no radiolabeled testosterone. The polymers present an appreciable affinity, with association constants of K(a) = 3.3 x 10(7) M(- 1) (high-affinity binding sites). The binding characteristics of the polymers were also evaluated in aqueous environment to study their viabilities as alternatives to antibodies in molecularly imprinted sorbent assays. Compared with the testosterone-specific antibodies present in commercial kits, our molecularly imprinted polymers are somewhat less sensitive but show a high selectivity.
Compiègne University of Technology, UMR CNRS 6022, B.P. 20529, 60205, Compiègne Cedex, France. jeanne.tse-sum-bui@utc.fr
Bernadette
B
Tse Sum Bui
Karsten
K
Haupt
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001665:Binding Sites; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D006801:Humans; D007118:Immunoassay / Q000592:standards; D054802:Molecular Imprinting / Q000379:methods; D054118:Nanospheres / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011944:Receptors, Androgen / Q000032:analysis* / Q000737:chemistry*; D013739:Testosterone / Q000032:analysis* / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000906:Antibodies; D011108:Polymers; D011944:Receptors, Androgen; D014867:Water; D013739:Testosterone