Muscle-bound primordial stem cells give rise to myofiber-associated myogenic and non-myogenic progenitors.
Myofiber cultures give rise to myogenic as well as to non-myogenic cells. Whether these myofiber-associated non-myogenic cells develop from resident stem cells that possess mesenchymal plasticity or from other stem cells such as mesenchymal stem cells (MSCs) remain unsolved. To address this question, we applied a method for reconstructing cell lineage trees from somatic mutations to MSCs and myogenic and non-myogenic cells from individual myofibers that were cultured at clonal density.Our analyses show that (i) in addition to myogenic progenitors, myofibers also harbor non-myogenic progenitors of a distinct, yet close, lineage; (ii) myofiber-associated non-myogenic and myogenic cells share the same muscle-bound primordial stem cells of a lineage distinct from bone marrow MSCs; (iii) these muscle-bound primordial stem-cells first part to individual muscles and then differentiate into myogenic and non-myogenic stem cells.
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel.
Elad
E
Segev
Gabi
G
Shefer
Rivka
R
Adar
Noa
N
Chapal-Ilani
Shalev
S
Itzkovitz
Inna
I
Horovitz
Yitzhak
Y
Reizel
Dafna
D
Benayahu
Ehud
E
Shapiro
D050156:Adipogenesis / Q000235:genetics; D000375:Aging / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D048430:Cell Shape; D002999:Clone Cells; D016000:Cluster Analysis; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D024510:Muscle Development / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D012333:RNA, Messenger