Broth from canned clams is suitable for use as an excellent source of free vitamin B12.
Vitamin B(12) was assayed and characterized in the broth of canned clams (boiled plain). The broth contained considerable amounts of vitamin B(12) (2.7-14.1 μg/100 g, 1.3-6.7 μg/can). HPLC and LC/ESI-MS/MS chromatograms demonstrated that the clam broth contained true vitamin B(12). Gel filtration experiments indicated that most (72%) of the vitamin B(12) found in the broth was recovered in free vitamin B(12) fractions. These results indicate that the clam broth would be suitable for use an excellent source of free vitamin B(12) for elderly persons with food-bound vitamin B(12) malabsorption.
Department of Science for Human Health, Shikoku University Junior College, Tokushima 771-1192, Japan.
Kazumi
K
Ueta
Shigeo
S
Takenaka
Yukinori
Y
Yabuta
Fumio
F
Watanabe
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D057141:Food, Preserved / Q000032:analysis*; D012758:Shellfish / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014805:Vitamin B 12 / Q000032:analysis*
D014805:Vitamin B 12