Association of the TNF-α -308G/A polymorphism with family history of type 2 diabetes mellitus in a Mexican population.
OBJECTIVE
We examined the possible association of the -308G/A polymorphism of the TNF-α promoter gene in type 2 diabetes mellitus (DM2) patients and in non-diabetic subjects with and without family history of DM2.


METHODS
We studied 87 non-diabetic subjects without DM2 family history in at least one of two generations, 48 non-diabetic subjects with DM2 family history and 95 DM2 patients. Genotyping was carried out by PCR-RFLP.


RESULTS
The frequency of TNF-α -308G/A genotype was significantly lower in non-diabetic subjects without DM2 relatives (6%) as compared to DM2 patients (24%) (odds ratio (OR)=5.24; 95% confidence interval (CI)=1.9-15.8, p<0.0005), but similar to non-diabetic subjects with DM2 relatives (29%) (OR=0.77; CI=0.3-1.7, p=0.4). Logistic regression analysis showed the association of TNF-α -308G/A polymorphism with DM2 family history (OR=5.80; CI=1.77-18.98, p<0.0003).


CONCLUSIONS
Our results suggest that TNF-α -308G/A polymorphism is associated with DM2 family history and is a risk factor for DM2.
Departamento de Ciencias Medicas, Universidad de Guanajuato, Mexico. elvaleticiaperez@yahoo.com
Elva
E
Perez-Luque
Juan Manuel
JM
Malacara
Ma Eugenia
ME
Garay-Sevilla
Martha Eugenia
ME
Fajardo
D000328:Adult; D000368:Aged; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000097:blood* / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha