Generation of a genotype VII Newcastle disease virus vaccine candidate with high yield in embryonated chicken eggs.
To generate a genotype VII Newcastle disease virus (NDV) vaccine with high yield in embryonated chicken eggs, we selected genotype VII NDV strain JS5/05, which possesses a high virus titer in embryos as the parental virus. Using reverse genetics, we generated a genetically tagged derivative (NDV/AI4) of JS5/05 by changing the amino acid sequence of the cleavage site of the F0 protein. Pathogenicity tests showed that NDV/AI4 was completely avirulent. NDV/AI4 was genetically stable and replicated efficiently during 10 consecutive passages in embryos. More importantly, serologic assays showed that oil-emulsion NDV/AI4 induced higher hemagglutination inhibition (HI) titers against the prevalent virus than oil-emulsion LaSota vaccine in chickens and geese. Moreover, NDV/AI4-induced HI titers rose faster than those elicited by LaSota in chickens. Both NDV/AI4 and LaSota provided protection against clinical disease and mortality after the challenge with the genotype VII NDV strain JS3/05. However, NDV/AI4 significantly reduced virus shedding from the vaccinated birds compared to LaSota. Taken together, these results suggest that NDV/AI4 can provide better protection than LaSota and is a promising vaccine candidate against genotype VII NDV.
Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu Province 225009, China.
Zenglei
Z
Hu
Shunlin
S
Hu
Chun
C
Meng
Xiaoquan
X
Wang
Jie
J
Zhu
Xiufan
X
Liu
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D005777:Geese; D005838:Genotype; D006385:Hemagglutination Inhibition Tests; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014760:Viral Fusion Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014774:Virulence; D017201:Virus Shedding
D014613:Vaccines, Attenuated; D014760:Viral Fusion Proteins; D014765:Viral Vaccines