Escherichia coli lipopolysaccharides and Staphylococcus aureus enterotoxin B differentially modulate inflammatory microRNAs in bovine monocytes.
MicroRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including activation of cells of the immune system. This study investigated the effect of Escherichia coli lipopolysaccharide (LPS) and Staphylococcus aureus enterotoxin B (SEB) on the expression of five miRNAs involved in the inflammatory response, including miR-9, miR-125 b, miR-155, miR-146 a and miR-223, in bovine CD14(+) cells (monocytes). Incubation of monocytes with SEB induced down-regulation of miR-155, miR-223 and miR-125 b, but not the anti-inflammatory miRNA miR-146 a. Conversely, incubation with LPS upregulated both miR-155 and miR-146 a. In vitro incubation of isolated CD14(+) bovine monocytes with LPS and SEB elicited different and opposite expression of miRNAs reportedly involved in inflammatory reactions.
Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Via Celoria 10, 20133 Milano, Italy.
Francesca
F
Dilda
Gloria
G
Gioia
Laura
L
Pisani
Laura
L
Restelli
Cristina
C
Lecchi
Francesca
F
Albonico
Valerio
V
Bronzo
Michele
M
Mortarino
Fabrizio
F
Ceciliani
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004768:Enterotoxins / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology*; D035683:MicroRNAs / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000378:metabolism*
D004768:Enterotoxins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D035683:MicroRNAs; C031818:enterotoxin B, staphylococcal