Herpesvirus saimiri encodes a new cytokine, IL-17, which binds to a novel cytokine receptor.
Herpesvirus Saimiri gene 13 (HVS13) exhibits 57% identity with the predicted sequence of a T cell-derived molecule termed CTLA8. Recombinant HVS13 and CTLA8 stimulate transcriptional factor NF-kappaB activity and Interleukin-6 (IL-6) secretion in fibroblasts, and costimulate T cell proliferation. An HVS13.Fc fusion protein was used to isolate a cDNA encoding a novel receptor that also binds CTLA8. This receptor is unrelated to previously identified cytokine receptor families. A recombinant soluble receptor inhibited T cell proliferation and IL-2 production induced by PHA, concanavalin A (conA), and anti-TCR MAb. These results define CTLA8 and HVS13 as novel cytokines that bind to a novel cytokine receptor. We propose to call these molecules IL-17, vIL-17, and IL-17R, respectively.
Immunex Cororation, 51 University Street, Seattle, Washington 98101, USA.
Zhengbin
Z
Yao
William C
WC
Fanslow
Michael F
MF
Seldin
Anne-Marie
AM
Rousseau
Sally L
SL
Painter
Michael R
MR
Comeau
Jeffrey I
JI
Cohen
Melanie K
MK
Spriggs
D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae; D001483:Base Sequence; D045744:Cell Line, Tumor; D006567:Herpesvirus 2, Saimiriine / Q000276:immunology*; D049673:History, 20th Century; D006801:Humans; D020381:Interleukin-17 / Q000266:history*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000276:immunology; D051381:Rats; D053722:Receptors, Interleukin-17 / Q000266:history*; D012097:Repressor Proteins / Q000266:history*; D015534:Trans-Activators / Q000266:history*
C098680:Il17a protein, mouse; C491673:Il17ra protein, mouse; D020381:Interleukin-17; C415247:ORF57 protein, Herpesvirus saimiri; D053722:Receptors, Interleukin-17; D012097:Repressor Proteins; D015534:Trans-Activators