A facile preparation method of a PFC-containing nano-sized emulsion for theranostics of solid tumors.
Theranostics means a therapy conducted in a diagnosis-guided manner. For theranostics of solid tumors by means of ultrasound, we designed a nano-sized emulsion containing perfluoropentane (PFC5). This emulsion can be delivered into tumor tissues through the tumor vasculatures owing to its nano-size, and the emulsion is transformed into a micron-sized bubble upon sonication through phase transition of PFC5. The micron-sized bubbles can more efficiently absorb ultrasonic energy for better diagnostic images and can exhibit more efficient ultrasound-driven therapeutic effects than nano-sized bubbles. For more efficient tumor delivery, smaller size is preferable, yet the preparation of a smaller emulsion is technically more difficult. In this paper, we used a bath-type sonicator to successfully obtain small PFC5-containing emulsions in a diameter of ca. 200nm. Additionally, we prepared these small emulsions at 40°C, which is above the boiling temperature of PFC5. Accordingly, we succeeded in obtaining very small nano-emulsions for theranostics through a very facile method.
Medical Engineering Laboratory, Research Center for Medical Science, The Jikei University School of Medicine, 3-25-8, Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan.
Kouichi
K
Shiraishi
Reiko
R
Endoh
Hiroshi
H
Furuhata
Masamichi
M
Nishihara
Ryo
R
Suzuki
Kazuo
K
Maruyama
Yusuke
Y
Oda
Jun-ichiro
J
Jo
Yasuhiko
Y
Tabata
Jun
J
Yamamoto
Masayuki
M
Yokoyama
D000818:Animals; D004339:Drug Compounding; D004655:Emulsions; D005260:Female; D005466:Fluorocarbons / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics; D013010:Sonication; D010420:Pentanes
D004655:Emulsions; D005466:Fluorocarbons; D011092:Polyethylene Glycols; C008806:perfluoropentane; D010420:Pentanes