Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil.
BACKGROUND
Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE).


METHODS
The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program.


RESULTS
Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%.


CONCLUSIONS
RT-PCR provides same day results, however it showed low sensitivity for VRE detection.
Weinmann Laboratório, Porto Alegre, Brazil.
Vlademir
V
Cantarelli
Bianca
B
Cavalcante
Diogo André
DA
Pilger
Fabiana
F
Souza
Cícero Gomes
CG
Dias
Teresa
T
Brodt
Maritza
M
Cantarelli
Carina
C
Secchi
Pedro Alves
PA
d'Azevedo
D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D016983:Enterococcus / Q000235:genetics* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; D019729:Carbon-Oxygen Ligases