In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts.
Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Teucrium polium and Teucrium chamaedrys. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellani trophozoites and cysts were decreased during the experimental process. Both extracts showed time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, T. chamaedrys showed the strongest amoebicidal effect on the trophozoites. In the presence of 16 mg/ml or above extract concentrations, no viable trophozoites were observed within 48 h. In the case of T. polium, no viable trophozoites were observed within 48 h at 32 mg/ml concentration. As expected, cysts were found more resistant to the extracts than the trophozoites.
Department of Molecular Biology and Genetics, Faculty of Science and Literature, Cumhuriyet University, 58140, Sivas, Turkey. bektastepe@yahoo.com
Bektas
B
Tepe
Erdogan
E
Malatyali
Serpil
S
Degerli
Seyda
S
Berk
D048248:Acanthamoeba castellanii / Q000187:drug effects*; D000563:Amebicides / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D033661:Oocysts / Q000187:drug effects; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D031367:Teucrium / Q000737:chemistry*; D013997:Time Factors; D053058:Trophozoites / Q000187:drug effects
D000563:Amebicides; D010936:Plant Extracts