Correlation study on expression of GST-π protein in brain tissue and peripheral blood of epilepsy rats induced by pilocarpine.
Previous studies have suggested that glutathione-S-transferase π (GST-π) over-expression in the brain tissue is associated with refractory epilepsy. However, whether the change in GST-π level in the peripheral blood is in line with that in brain tissue remains unknown. This study examined the correlation between GST-π in brain tissue and that in peripheral blood in rat models of pilocarpine-induced refractory epilepsy. The animals were divided into drug-resistant group and drug-responsive group according to the response to anti-epileptic drugs. GST-π expression in brain tissue was immunohistochemically determined, while the expression of GST-π in peripheral blood was analyzed by Western blotting. In the hippocampus and cortex, GST-π was mainly found in the cytoplasm and membrane of neurons, and the GST-π expression level was higher in drug-resistant group than in the drug-responsive group and saline control group (P<0.05). Moreover, there was no significant difference between responders and saline control animals (P>0.05). The change in expression of GST-π in peripheral blood showed the same pattern as that in brain tissues, suggesting GST-π might contribute to drug resistance in epilepsy. Importantly, the GST-π over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents.
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, 430022, China. dengxuejun1965@126.com.
Xuejun
X
Deng
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, 430022, China.
Hong
H
Jia
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, 430022, China.
Zhiyong
Z
Yang
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, 430022, China.
Gang
G
Li
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan, 430022, China.
Shenggang
S
Sun
D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D004351:Drug Resistance / Q000502:physiology; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D051549:Glutathione S-Transferase pi / Q000097:blood* / Q000378:metabolism*; D008297:Male; D010862:Pilocarpine; D051381:Rats
D000927:Anticonvulsants; D015415:Biomarkers; D010862:Pilocarpine; D051549:Glutathione S-Transferase pi; C496558:Gstp1 protein, rat