Enzymatic glucose biosensor based on CeO2 nanorods synthesized by non-isothermal precipitation.
Cerium oxide nanorods (CeO(2) NRs) were synthesized without templates through a low cost and simple non-isothermal precipitation method. The structure and morphology of CeO(2) NRs were characterized by X-ray diffraction and transmission electron microscopy. The CeO(2) NRs films, deposited on indium tin oxide (ITO)-coated glass substrates through electrophoretic deposition, were used for the immobilization of glucose oxidase (GOx). Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy were used to characterize the CeO(2) NRs/ITO and GOx/CeO(2) NRs/ITO electrodes. The GOx/CeO(2) NRs/ITO electrode exhibits a linear range for the detection of glucose from 2 to 26 mM (correlation coefficient: 0.99) at 1-2s response time. Biosensor sensitivity is 0.165 μA mM(-1) cm(-2) with 100 μM detection limit. The anti-interference ability of the biosensor was also examined. The mediator-less application of CeO(2) NRs for glucose sensing was demonstrated.
Department of Materials Science and Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea. pdewyani@yahoo.in
Dewyani
D
Patil
Nguyen Quoc
NQ
Dung
Hyuck
H
Jung
Se Yong
SY
Ahn
Dong Mi
DM
Jang
Dojin
D
Kim
D015374:Biosensing Techniques / Q000295:instrumentation*; D002563:Cerium / Q000737:chemistry*; D011232:Chemical Precipitation; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000032:analysis* / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D004800:Enzymes, Immobilized; D002563:Cerium; C030583:ceric oxide; D005949:Glucose Oxidase; D005947:Glucose