Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells.
Association of herpesvirus DNA with histones has important implications for lytic and latent infections; thus herpesviruses arbitrate interactions with histones to productively infect host cells. While regulation of alpha and betaherpesvirus chromatin during lytic infection has been actively investigated, very little is known about interaction of gammaherpesvirus DNA with histones upon de novo lytic infection. Murine gammaherpesvirus-68 (MHV68) is a rodent pathogen that offers a tractable system to study gammaherpesvirus lytic infection in primary cells. In this study we report that MHV68 promoter and orilyt sequences underwent dynamic association with histone H3 during de novo lytic infection of primary macrophages and fibroblasts. Similar to HSV-1, the degree of MHV68 DNA association with histone H3 was dependent on the multiplicity of infection and was further regulated by viral DNA synthesis. Our work sets a precedent for future studies of gammaherpesvirus chromatin during de novo lytic infection.
Department of Microbiology and Molecular Genetics, Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Bryan C
BC
Mounce
Fei Chin
FC
Tsan
Sarah
S
Kohler
Lisa A
LA
Cirillo
Vera L
VL
Tarakanova
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000821:virology; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000821:virology; D015967:Gene Expression Regulation, Viral; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D011401:Promoter Regions, Genetic; D018144:Rhadinovirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics
D002843:Chromatin; D004279:DNA, Viral; D006657:Histones; D014764:Viral Proteins