Silencing of miR-124 induces neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis through promoting AHR.
Neuroblastoma is the most common extracranial solid tumor in children. We investigate whether miR-124, the abundant neuronal miRNA, plays a pivotal role in neuroblastoma. Knockdown of miR-124 promotes neuroblastoma SK-N-SH cell differentiation, cell cycle arrest and apoptosis. Further miR-124 is predicted to target aryl hydrocarbon receptor (AHR) which may promote neuroblastoma cell differentiation. We validate that miR-124 may suppress the expression of AHR by targeting its 3'-UTR. These results suggest that miR-124 could serve as a potential therapeutic target of neuroblastoma.
Department of Life Science, National Taiwan University, Taipei, Taiwan.
Tsui-Chin
TC
Huang
Hsin-Yi
HY
Chang
Cheng-Yu
CY
Chen
Pei-Yi
PY
Wu
Hsinyu
H
Lee
Yung-Feng
YF
Liao
Wen-Ming
WM
Hsu
Hsuan-Cheng
HC
Huang
Hsueh-Fen
HF
Juan
D017209:Apoptosis; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism
C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D018336:Receptors, Aryl Hydrocarbon