The detection and differentiation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus endocarditis by using the BD GeneOhm StaphSR Assay.
We use the BD GeneOhm StaphSR Assay (BD Diagnostics, Oakville, Canada) to screen for Staphylococcus aureus nasal colonization and sought to evaluate this assay for the assessment of valve specimens from patients with endocarditis. We examined 23 paired fresh and formalin-fixed, paraffin-embedded cardiac valve tissue samples, 12 of which had S aureus endocarditis, using the BD GeneOhm StaphSR Assay for the detection and differentiation of methicillin-susceptible and methicillin-resistant S aureus. This assay appropriately characterized all specimens with respect to the presence or absence of S aureus. There was an 87.5% correlation between the presence or absence of the mecA gene and the oxacillin susceptibility results for the S aureus isolates studied. The GeneOhm StaphSR assay accurately detected S aureus in cardiac valve tissue samples. Rare discordances were observed between oxacillin susceptibility status and mecA gene detection by this assay.
Molecular Pathology/L11, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195.
Amy B
AB
Frey
Deborah A
DA
Wilson
Margaret M
MM
LaSalvia
Carmela D
CD
Tan
E Rene
ER
Rodriguez
Nabin K
NK
Shrestha
Gerri S
GS
Hall
Gary W
GW
Procop
D001431:Bacteriological Techniques / Q000379:methods; D004697:Endocarditis, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D006351:Heart Valves / Q000382:microbiology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification
D011933:Reagent Kits, Diagnostic