Production of 3-hydroxypropionate homopolymer and poly(3-hydroxypropionate-co-4-hydroxybutyrate) copolymer by recombinant Escherichia coli.
Conversion of 3-hydroxypropionate (3HP) from 1,3-propanediol (PDO) was improved by expressing dehydratase gene (dhaT) and aldehyde dehydrogenase gene (aldD) of Pseudomonas putida KT2442 under the promoter of phaCAB operon from Ralstonia eutropha H16. Expression of these genes in Aeromonas hydrophila 4AK4 produced up to 21 g/L 3HP in a fermentation process. To synthesize homopolymer poly(3-hydroxypropionate) (P3HP), and copolymer poly(3-hydroxypropionate-co-3-hydroxybutyrate) (P3HP4HB), dhaT and aldD were expressed in E. coli together with the phaC1 gene encoding polyhydroxyalkanoate (PHA) synthase gene of Ralstonia eutropha, and pcs' gene encoding the ACS domain of the tri-functional propionyl-CoA ligase (PCS) of Chloroflexus aurantiacus. Up to 92 wt% P3HP and 42 wt% P3HP4HB were produced by the recombinant Escherichia coli grown on PDO and a mixture of PDO+1,4-butanediol (BD), respectively.
Department Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Qin
Q
Zhou
Zhen-Yu
ZY
Shi
De-Chuan
DC
Meng
Qiong
Q
Wu
Jin-Chun
JC
Chen
Guo-Qiang
GQ
Chen
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000235:genetics / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis; D002072:Butylene Glycols / Q000378:metabolism; D041861:Chloroflexus / Q000235:genetics / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D020118:Cupriavidus necator / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011091:Polyesters / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011409:Propylene Glycols / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism
D001704:Biopolymers; D002072:Butylene Glycols; D006885:Hydroxybutyrates; D011091:Polyesters; D011409:Propylene Glycols; C570666:poly(3-hydroxypropionate-co-4-hydroxybutyrate); C111420:4-hydroxybutyric acid; D019344:Lactic Acid; C041787:1,3-propanediol; C039681:1,4-butanediol; C031601:hydracrylic acid; D000444:Aldehyde Dehydrogenase; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase; D006836:Hydro-Lyases; D003066:Coenzyme A Ligases; C068072:propionate - CoA ligase