An immunochemical method for detection and analysis of changes in methylome.
DNA methylation is an important epigenetic modification involved in the ability of an organism to respond to stress and adaptation. It has been implicated in development, differentiation, oncogenesis, chromatin remodelling, nutrigenomics, and appears to play a pivotal role in many regulatory and adaptive functions. It is therefore important to analyze the status of DNA methylation and its changes under various developmental, carcinogenic, pharmacological, and environmental conditions. In this report we describe an immunochemical method for the detection of genome wide DNA methylation and its alterations under various conditions along with the analysis of DNA methyltransferase activity. The ability of this approach to detect and provide a map of methylomic changes in a genome facilitates assessment of various agents and conditions which can alter this important epigenetic signal. This experimental system permits rapid evaluation of potential target genes which would be modulated by DNA methylation changes and thus the gene networks that govern the processes.
Department of Zoology, Centre for Advanced Studies, University of Pune, Pune 411007, India. deepti@bioinfo.ernet.in
Deepti D
DD
Deobagkar
Chitra
C
Panikar
Shriram N
SN
Rajpathak
Nayana S
NS
Shaiwale
Sanjay
S
Mukherjee
D000818:Animals; D001921:Brain / Q000201:enzymology; D002460:Cell Line; D003596:Cytosine / Q000737:chemistry; D019175:DNA Methylation / Q000528:radiation effects*; D015254:DNA Modification Methylases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000737:chemistry; D004789:Enzyme Activation; D057075:Enzyme Assays; D044127:Epigenesis, Genetic; D005260:Female; D016678:Genome; D007120:Immunochemistry / Q000379:methods*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011827:Radiation; D012680:Sensitivity and Specificity
D003596:Cytosine; D015254:DNA Modification Methylases