Millifluidic droplet analyser for microbiology.
We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (≥10(3)) aqueous emulsion droplets (~100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of ~10(6) cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition.
UPMC Université Paris 06, CNRS UMR 7195, ESPCI ParisTech, Paris, France. larysa.baraban@espci.fr
Larysa
L
Baraban
Fabien
F
Bertholle
Merijn L M
ML
Salverda
Nicolas
N
Bremond
Pascal
P
Panizza
Jean
J
Baudry
J Arjan G M
JA
de Visser
Jérôme
J
Bibette
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002439:Cefotaxime / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009821:Oils / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000900:Anti-Bacterial Agents; D009821:Oils; D014867:Water; D002439:Cefotaxime