Synthesis and characterization of novel biodegradable and electroactive hydrogel based on aniline oligomer and gelatin.
A biodegradable electroactive hydrogel (AP-g-GA), aniline pentamer (AP) grafting gelatin (GA), is synthesized by a coupling reaction between the carboxyl group of AP and the amino side group of GA in aqueous solution. The electroactivity of the physical hydrogel is confirmed by UV-vis and CV. The hydrophobic AP changes the hydrogel's porous structure of the natural GA and the gel-time, which is confirmed by the rheological behavior of the AP-g-GA and GA. With an increase in the content of AP, the hydrogel gradually forms a porous structure, from "honeycomb" to "bamboo raft". The porous scaffolds can be crosslinked with 3.5% EDC in 90% ethanol. MTT assays show that the AP-g-GA exhibits reduced cytotoxicity compared to EM AP due to the introduction of the biocompatible GA moiety. The in vitro cell cultures suggest that the AP-g-GA#1 (with 1.9% AP) shows the best biocompatibility and cell adhesion ability.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Yadong
Y
Liu
Jun
J
Hu
Xiuli
X
Zhuang
Peibiao
P
Zhang
Yen
Y
Wei
Xianhong
X
Wang
Xuesi
X
Chen
D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002234:Carbodiimides / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D004553:Electric Conductivity; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D051381:Rats; D013053:Spectrophotometry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D000814:Aniline Compounds; D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011108:Polymers; D005780:Gelatin; C023650:aniline