Differential expression and function of AR isoforms in prostate cancer.
The androgen receptor (AR) plays a key role in prostate cancer (PCa). Two             isoforms of AR, are AR-A and -B, which differ by a lack of the first 187 amino             acids in the NH2-terminal transactivation domain of AR-A. Since little is known             about the expression and basic function of the AR-A/B isoforms in prostate cancer,             the aim of this study was to analyze this possible association. The AR-A, -B and             AR-A/B ratio was determined in the tissues of healthy controls, benign prostate             hyperplasia (BPH) and PCa by means of Western blot analysis and immunofluorescence.             The elevation of AR-A, and -B, as well as the AR-A/B ratio with regard to Gleason             scores, were assessed in prostate cancer compared to BPH and normal prostate.             In order to further investigate the role of AR A/B isoform function, we transfected             PC3 cells with an AR or AR-A expression vector. The overexpression of AR-A and             -B significantly decreased the invasion and proliferation of PC3 cells. However,             the overexpression of AR-A further decreased proliferation but accelerated the             invasion of PC3 cells compared to AR-B. In conclusion, the elevation of AR-A and             -B, and the AR-A/B ratio, is associated with prostate cancer occurrence and progression.             Furthermore, AR-A could provide a new potential therapy with regard to the decrease             in the invasion and proliferation of prostate cancer cells. Our study provides             insight into further understanding the biological role of AR-A in its interaction             with AR-B and its impact on PCa clinical treatment.
Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
Rui
R
Zeng
Zhiyong
Z
Liu
Yinghao
Y
Sun
Chuanliang
C
Xu
D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*
D020033:Protein Isoforms; D011944:Receptors, Androgen