Isolation and characterization of microsatellite loci in Sassafras randaiense (Lauraceae).
OBJECTIVE
Microsatellite loci were developed in Sassafras randaiense, an endemic tree species in Taiwan, to investigate the conservation genetics of this invaluable plant that is at risk for continuing population decline.


RESULTS
Using a magnetic bead enrichment method, 27 primer pairs amplifying di-, tri, and tetranucleotide repeats were developed. Across the populations tested, 16 primer sets showed polymorphism, with an average of 4.06 alleles per polymorphic locus. Twenty of the 27 developed primer sets were also successfully amplified in its congener S. tzumu from China.


CONCLUSIONS
The new primer sets described here are a powerful tool for investigating the spatial patterns of genetic diversity and for designing effective management strategies for this invaluable plant.
School of Forestry and Resource Conservation, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Daan, Taipei 10617 Taiwan. kuofangchung@ntu.edu.tw
Kuo-Fang
KF
Chung
Ting-Ting
TT
Lin
Yung-Shin
YS
Tsai
Shu-Tzong
ST
Lin
Ching-I
CI
Peng
D000483:Alleles; D002681:China; D017931:DNA Primers; D018744:DNA, Plant; D056426:Genetic Loci; D005828:Genetics, Population; D005843:Geography; D006579:Heterozygote; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D027441:Sassafras / Q000235:genetics*; D013045:Species Specificity; D013624:Taiwan; D014197:Trees / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant