Chemical structure of wall teichoic acid isolated from Enterococcus faecium strain U0317.
Wall teichoic acid (WTA) was isolated from Enterococcus faecium strain U0317 and structurally characterized using (1)H, (13)C, and (31)P NMR spectroscopy, including two-dimensional COSY, TOCSY, ROESY, HMQC, and HMBC experiments. Further compositional determination was undertaken using classical chemical methods and HF treatment followed by GLC and GLC-MS analyses. The repeating unit of WTA consisted of two residues of 2-acetamido-2-deoxy-D-galactose, glycerol (Gro), and phosphate, and has the structure shown below: [See formula in text].
Faculty of Chemistry, University of Gdansk, Sobieskiego 18, PL-80-952 Gdansk, Poland.
Anna
A
Bychowska
Christian
C
Theilacker
Małgorzata
M
Czerwicka
Kinga
K
Marszewska
Johannes
J
Huebner
Otto
O
Holst
Piotr
P
Stepnowski
Zbigniew
Z
Kaczyński
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D016984:Enterococcus faecium; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013682:Teichoic Acids / Q000737:chemistry* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid