[Correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis].
OBJECTIVE
Study on correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis.


METHODS
The content of baicalin and baicalein in suspension cell of S. baicalensis was determined by HPLC. The content of total flavonoids and H2O2, the activity of POD and PAL was detected by UV spectrophotometry.


RESULTS
The content of total flavonoid and the activity of PAL increased significantly in 12 days after 40 degrees C, dark and PEG stress. Around 12 days after NPA, NPA +40 degrees C, 40 degrees C, NPA + dark, dark and PEG stress, the content of baicalin declined and the content of baicalein rise, the activity of POD showed an increasing trend, and level of H2O2 remain stable.


CONCLUSIONS
Moderate environmental stress could promote the accumulation of total flavonoids in S. baicalensis, baicalin convert to baicalein by POD, and maintaining the stability of H2O2 content to avoid oxidative damage.
Wuhan Polythechnic University, Wuhan 430023, China.
Lingfei
L
Shuai
Yuan
Y
Yuan
Ping
P
Chen
Shufang
S
Lin
D000890:Anti-Infective Agents / Q000032:analysis / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002478:Cells, Cultured; D003624:Darkness; D044950:Flavanones / Q000032:analysis / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010946:Plants, Medicinal; D011092:Polyethylene Glycols / Q000494:pharmacology; D031364:Scutellaria baicalensis / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013312:Stress, Physiological
D000890:Anti-Infective Agents; D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D016877:Oxidants; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; C038044:baicalin; D011092:Polyethylene Glycols; C006680:baicalein; D006861:Hydrogen Peroxide; D009195:Peroxidase; D010650:Phenylalanine Ammonia-Lyase