[Apoptosis and expression of Fas/FasL in tumor infiltrating dendritic cells in human endometrioid adenocarcinoma].
OBJECTIVE
To investigate apoptosis of tumor infiltrating dendritic cells (TIDC) and their expression of Fas/FasL (CD95/CD95L) in human endometrioid adenocarcinoma.


METHODS
The apoptotic rate of TIDC was measured in 45 cases of endometrioid adenocarcinoma and 20 cases of normal endometrium tissues (control) by double-label immunohistochemistry using the monoclonal antibody S-100 protein and TUNEL technique. The expressions of Fas and FasL in TIDCs were detected using double-label immunohistochemistry and imaging analysis.


RESULTS
The apoptotic rate of TIDCs in endometrioid adenocarcinoma were significantly higher than that in normal endormetrium [(13.02∓0.64)% vs (6.82∓0.53)%, P<0.05]. The expression levels of Fas in the TIDCs were significantly lower, whereas FasL expression significantly higher in endometrioid adenocarcinoma than in normal endormetrium (7.88∓1.05 vs 19.25∓3.03, P<0.05; 12.95∓2.25 vs 7.51∓1.14, P<0.05).


CONCLUSIONS
Increased apoptosis of the TIDCs and abnormal expression of Fas/FasL in TIDCs in endometrioid adenocarcinoma may lead to tumor immune escape.
Department of Obstetrics and Gynecology, Jinan University, Guangzhou, China. jiajianjun615@sohu.com
Jian-jun
JJ
Jia
Zi-neng
ZN
Wang
Ge-xiu
GX
Liu
Zhi-xin
ZX
Wang
D017209:Apoptosis / Q000502:physiology*; D018269:Carcinoma, Endometrioid / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D003713:Dendritic Cells / Q000276:immunology*; D016889:Endometrial Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D019139:Tumor Escape; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor