Association of genetic polymorphisms of glutathione-S-transferase genes (GSTT1, GSTM1, and GSTP1) and susceptibility to nonalcoholic fatty liver disease in Zahedan, Southeast Iran.
Oxidative damage is thought to play a pivotal role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Glutathione-S-transferases (GSTs) are involved in cell protection against oxidative stress. We examined whether GSTM1, GSTT1, and GSTP1 polymorphisms are associated with NAFLD in a sample of the Iranian population. The current case-control study included 83 patients with NAFLD and 93 healthy subjects. The GSTM1 and GSTT1 polymorphisms were analyzed by multiplex polymerase chain reaction (PCR). The GSTP1 polymorphism was detected by tetra amplification refractory mutation system-PCR assay. The GSTM1-null genotype was significantly associated with the development of NAFLD (odds ratios [OR]=2.171, 95% confidence intervals [CI]=1.188-3.970, p=0.015). The GSTP1 Val allele was shown to be a risk factor for NAFLD (OR=1.739, 95% CI=1.089-2.777, p=0.024). The GSTT1 polymorphism was not significantly different between control and patient groups (p=0.221). This study showed that GSTM1 and GSTP1, but not GSTT1, genetic polymorphisms are associated with NAFLD in a sample of the Iranian population, and may be used to determine the risk of development of NAFLD.
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. mhd.hashemi@gmail.com
Mohammad
M
Hashemi
Ebrahim
E
Eskandari-Nasab
Aliakbar
A
Fazaeli
Ali
A
Bahari
Noor-Allah
NA
Hashemzehi
Sara
S
Shafieipour
Mohsen
M
Taheri
Abdolkarim
A
Moazeni-Roodi
Zahra
Z
Zakeri
Alireza
A
Bakhshipour
Saeid
S
Ghavami
D000328:Adult; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics; D005234:Fatty Liver / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007492:Iran; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D065626:Non-alcoholic Fatty Liver Disease; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D012307:Risk Factors
D004247:DNA; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human