Control of nanoparticle size, reactivity and magnetic properties during the bioproduction of magnetite by Geobacter sulfurreducens.
The bioproduction of nanoscale magnetite by Fe(III)-reducing bacteria offers a potentially tunable, environmentally benign route to magnetic nanoparticle synthesis. Here, we demonstrate that it is possible to control the size of magnetite nanoparticles produced by Geobacter sulfurreducens by adjusting the total biomass introduced at the start of the process. The particles have a narrow size distribution and can be controlled within the range of 10-50 nm. X-ray diffraction analysis indicates that controlled production of a number of different biominerals is possible via this method including goethite, magnetite and siderite, but their formation is strongly dependent upon the rate of Fe(III) reduction and total concentration and rate of Fe(II) produced by the bacteria during the reduction process. Relative cation distributions within the structure of the nanoparticles have been investigated by x-ray magnetic circular dichroism and indicate the presence of a highly reduced surface layer which is not observed when magnetite is produced through abiotic methods. The enhanced Fe(II)-rich surface, combined with small particle size, has important environmental applications such as in the reductive bioremediation of organics, radionuclides and metals. In the case of Cr(VI), as a model high-valence toxic metal, optimized biogenic magnetite is able to reduce and sequester the toxic hexavalent chromium very efficiently to the less harmful trivalent form.
School of Earth, Atmospheric and Environmental Sciences and Williamson Research Centre for Molecular Environmental Science, University of Manchester, Manchester M13 9PL, UK. James.Byrne@postgrad.manchester.ac.uk
J M
JM
Byrne
N D
ND
Telling
V S
VS
Coker
R A D
RA
Pattrick
G
G
van der Laan
E
E
Arenholz
F
F
Tuna
J R
JR
Lloyd
D002857:Chromium; D002942:Circular Dichroism; D052203:Ferrosoferric Oxide / Q000378:metabolism*; D045302:Geobacter / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D060306:Magnetometry; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D010316:Particle Size; D014961:X-Ray Diffraction
D058185:Magnetite Nanoparticles; D002857:Chromium; D052203:Ferrosoferric Oxide