HLA-B*15:33, a rare allele whose product reacts as an HLA-B62 and -Cw5/Cw8 specificity.
Using PCR with sequence-specific primers (SSP) and subsequent sequencing of exons 2 and 3, we identified an example of B*15:33 in a likely north-western European Caucasoid volunteer haemopoietic stem cell (HSC) donor. This was only the second example submitted to the IMGT/HLA database since B*15:33 was first described in 1996. B*15:33 differs from B*15:01:01:01 by three nucleotides resulting in two amino acid differences with B*15:01 (131S>R, 138T>K). This allele encodes a typical HLA-B62 specificity--as confirmed using 17 local antisera from parous women and 19 monoclonal antibodies directed towards B15, B62 and B63 specificities. Importantly, it also reacted as a Cw5/Cw8 specificity when tested against 21 Cw5, Cw5/Cw8 and Cw8 antisera. This Cw5/Cw8 reactivity is probably due to the B*15:33 specificity having lysine at position 138 which is possessed by numerous C*05 and many C*08 products. B*15:33 is likely to have derived from a HLA-B/C inter-locus gene conversion event. HLA-B*15:33 is clearly rare--this single example was found during the HLA PCR-SSP-based typing of 57,079 potential HSC donors. This indicates an allele frequency of <0.00001 in blood donors resident in Wales. An Epstein-Barr virus transformed B-cell line from the HLA-B*15:33 donor is available.
Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK.
J
J
Street
T
T
Climer
J
J
Johnson
C
C
Darke
D000483:Alleles; D020816:Amino Acid Motifs; D000937:Antigen-Antibody Reactions; D001483:Base Sequence; D002461:Cell Line, Transformed; D017931:DNA Primers / Q000235:genetics; D000939:Epitopes; D005091:Exons; D005260:Female; D005787:Gene Frequency; D059910:HLA-B15 Antigen / Q000235:genetics* / Q000276:immunology; D015236:HLA-C Antigens / Q000235:genetics / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014019:Tissue Donors
D017931:DNA Primers; D000939:Epitopes; D059910:HLA-B15 Antigen; D015236:HLA-C Antigens; C037505:HLA-C*05 antigen; C524551:HLA-C*08 antigen