Characterization of a highly polymeric proanthocyanidin fraction from persimmon pulp with strong Chinese cobra PLA2 inhibition effects.
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin, epigallocatechin, (epi)gallocatechin-3-O-gallate, and (epi)catechin-3-O-gallate occurred as extender units. The mean degree of polymerization was 23.7. MALDI TOF/MS, thioly-HPLC, FTIR and circular dichroism (CD) analyses showed that the fraction had high prodelphinidin content (55%) and a very high degree of 3-O-galloylation (92%). A type linkage is dominant in it and it had 4β linkage of the flavanyl substituent and 4R absolute configuration.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Shu-fen
SF
Xu
Bo
B
Zou
Jie
J
Yang
Ping
P
Yao
Chun-mei
CM
Li
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D029788:Diospyros / Q000737:chemistry*; D017815:Elapidae / Q000502:physiology; D005260:Female; D005638:Fruit / Q000737:chemistry*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D051379:Mice; D015394:Molecular Structure; D064801:Phospholipase A2 Inhibitors; D053147:Plant Exudates / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D030162:Reptilian Proteins / Q000037:antagonists & inhibitors; D012910:Snake Venoms / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D064801:Phospholipase A2 Inhibitors; D053147:Plant Exudates; D011108:Polymers; D044945:Proanthocyanidins; D030162:Reptilian Proteins; D012910:Snake Venoms