Optimization of culture conditions of the thraustochytrid Aurantiochytrium sp. strain 18W-13a for squalene production.
Optimum conditions of temperature, salinity and glucose concentration were investigated for squalene production of the strain of Aurantiochytrium sp. 18 W-13a, with a high content of squalene. Squalene production by this strain was optimum at 25 °C, 25-50% seawater concentration and 2-6% glucose concentration. When this strain was grown in the optimum condition, the squalene content and production of approximately 171 mg/g dry weight and 0.9 g/L were much higher than that previously reported in thraustochytrids, plants and yeasts, respectively. Therefore, 18 W-13a could be used as an alternative source of commercial squalene.
Graduate School of Life and Environmental Sciences, University of Tsukuba 1-1-1, Tennodai, Tsukuba 305-8572, Japan. anakazawa@ies.life.tsukuba.ac.jp
Atsushi
A
Nakazawa
Hiroshi
H
Matsuura
Ryoji
R
Kose
Syou
S
Kato
Daiske
D
Honda
Isao
I
Inouye
Kunimitsu
K
Kaya
Makoto M
MM
Watanabe
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D010802:Phylogeny; D013185:Squalene / Q000378:metabolism*; D058009:Stramenopiles / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D003470:Culture Media; D008055:Lipids; D013185:Squalene