The mechanism of the second step of pre-mRNA splicing.
The molecular mechanisms of the second step of pre-mRNA splicing in yeast and higher eukaryotes are reviewed. The important elements in the pre-mRNA, the participating proteins, and the proposed secondary structures and roles of the snRNAs are described. The sequence of events in the second step is presented, focusing on the actions of the proteins in setting up and facilitating the second reaction. Mechanisms for avoiding errors in splicing are discussed.
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. dhorowitz@usuhs.mil
David S
DS
Horowitz
D053487:DEAD-box RNA Helicases / Q000378:metabolism; D005091:Exons; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism*; D022821:RNA Splice Sites; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism
D012322:RNA Precursors; D022821:RNA Splice Sites; D012342:RNA, Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D053487:DEAD-box RNA Helicases