HupW protease specifically required for processing of the catalytic subunit of the uptake hydrogenase in the cyanobacterium Nostoc sp. strain PCC 7120.
The maturation process of [NiFe] hydrogenases includes a proteolytic cleavage of the large subunit. We constructed a mutant of Nostoc strain PCC 7120 in which hupW, encoding a putative hydrogenase-specific protease, is inactivated. Our results indicate that the protein product of hupW selectively cleaves the uptake hydrogenase in this cyanobacterium.
Department of Photochemistry and Molecular Science, The Ångström Laboratories, Uppsala University, Uppsala, Sweden.
Pia
P
Lindberg
Ellenor
E
Devine
Karin
K
Stensjö
Peter
P
Lindblad
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D046937:Nostoc / Q000201:enzymology* / Q000235:genetics; D010088:Oxidoreductases / Q000378:metabolism*
D001426:Bacterial Proteins; D010088:Oxidoreductases; C029746:uptake hydrogenase; D010450:Endopeptidases