Identification of Dp71e, a new dystrophin with a novel carboxy-terminal end.
Several dystrophin Dp71 isoforms have previously been described and can be grouped into two subfamilies (Dp71d or Dp71f) depending upon the splicing of exon 78. As a consequence of this splicing, each group has a carboxy-terminal end with a unique amino acid composition; this composition imparts specific characteristics with respect to subcellular localization and interactions with particular members of the dystrophin-associated proteins (DAPs) complex. We have discovered a new alternative splicing event at the 3' region of the Dp71 transcript. This spliced region has a unique sequence that codes for 10 amino acids and prevents the translation of exons 78 and 79. This novel Dp71 isoform is called Dp71e and is expressed in undifferentiated cells and during nerve growth factor-induced differentiation of PC12 cells. Interestingly, Dp71e mRNA and protein expression increase during PC12 cell differentiation mediated by NGF. This new Dp71 isoform is also expressed in rat organs and in human cell lines.
Department of Genetics & Molecular Biology, CINVESTAV: Research Centre for Advanced Studies, IPN, México, Mexico.
Abril
A
Saint Martín
Jorge
J
Aragón
Francisco
F
Depardon-Benítez
Alejandra
A
Sánchez-Trujillo
Guillermo
G
Mendoza-Hernández
Victor
V
Ceja
Cecilia
C
Montañez
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D016189:Dystrophin / Q000235:genetics* / Q000378:metabolism*; D005091:Exons / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013347:Subcellular Fractions
D016189:Dystrophin; D020033:Protein Isoforms; D012333:RNA, Messenger; C085339:apo-dystrophin 1; D020932:Nerve Growth Factor