[Establishment and applicability evaluation of animal model which was suitable to evaluate immediate hypersensitivity induced by injections of traditional Chinese medicine in BN rats].
Qingkailing injection, Shuanghuanglian injection, baicalin, chlorogenic acid as sample, guinea pig as control, to observe the specificity of allergic response to traditional Chinese medicine (TCM) injection in BN rats and establish a suitable animal model to evaluate applicability of allergic response in BN rats and guinea pigs induced by TCM. BN rats were sensitized by TCM injection, the symptoms, the rate and degree of allergic response were observed, the level of histamine in serum and tissues were determined by ELISA assay, the rate and degree of pathological changes in target organs were observed by HE staining under light microscope. There were significant symptoms of allergic response can be in BN rats, the level of histamine in serum, lung and trachea tissues increased significantly and there were significant pathological changes in lungs and tracheas. Meanwhile, the similar symptoms of allergic response can be induced by penicillin and trichosanthin. The rate and degree of allergic response, the rate and degree of pathological changes was higher in BN rats than in guinea pigs. Compared with guinea pig, BN rat is probably more suitable animal model in evaluating allergic response to injection of TCM.
Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. ruochushan@sina.com
Shanshan
S
Guo
Yizhong
Y
Wang
Yahong
Y
Jin
Yi
Y
Zhang
Yingjie
Y
Gao
Yujing
Y
Shi
Xiaolan
X
Cui
D000818:Animals; D004195:Disease Models, Animal; D004342:Drug Hypersensitivity; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000009:adverse effects*; D006168:Guinea Pigs; D006969:Hypersensitivity, Immediate / Q000139:chemically induced*; D007267:Injections; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D011914:Rats, Inbred BN
D004365:Drugs, Chinese Herbal