Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion.
OBJECTIVE
To evaluate and compare standard sperm parameters and sperm chromatin integrity by sperm chromatin dispersion test (SCD) in ejaculates from men whose partners have a history of recurrent pregnancy loss and from control group of fertile men.


METHODS
Thirty couples with unexplained recurrent abortion (case group) and 30 fertile couples (control group) referring to Shiraz infertility center were included. Sperm parameters were assessed in semen samples from two groups and then staining with SCD procedure. The results were analyzed by performing ANOVA and Tukey(,)s tests.


RESULTS
In control group, nucleoids with big (65.93 ± 2.35), small (12.4 ± 0.60) and without halo (11.6 ± 0.50) showed significant difference with case group (41.40 ± 1.43), (21.16 ± 1.11) and (23.26 ± 1.10) respectively. In the RPL group spermatozoa with high percentage of abnormal parameters (morphology and motility) was observed (p ≤ 0.05).


CONCLUSIONS
This study strengthens the current literature associating sperm quality with recurrent pregnancy loss, and emphasizes the important of evaluating male factor by tests such as SCD in addition to conventional sperm parameters.
Department of Anatomical sciences, Ahvaz Jundishapor University of Medical Science, P.O. Box: 61357-15794, Ahvaz, Iran, forouzan_absalan@yahoo.com
Forouzan
F
Absalan
Alireza
A
Ghannadi
Marjaneh
M
Kazerooni
Rostami
R
Parifar
Fatemeh
F
Jamalzadeh
Sahar
S
Amiri
D000026:Abortion, Habitual / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D013076:Sperm Count; D013081:Sperm Motility / Q000235:genetics; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*
D002843:Chromatin