Circadian variation of cell proliferation in HTR-8/SVneo cell line.
Circadian clock controls several physiological processes such as cell proliferation. Extravillous trophoblast proliferation is a tightly regulated function playing a fundamental role in maternal vessel remodeling. We recently demonstrated that clock genes Per2 and Dec1 as well as the clock-controlled genes Dbp and Vegf are rhythmically expressed in human extravillous trophoblast-derived HTR-8/SVneo cells. Analyzing the time course of HTR-8/SVneo cell proliferation, a circadian variation in cell number was found. Moreover, we showed a rhythmic expression of mRNAs for Wee1 and stathmin, two genes involved in cell cycle progression. We suggest that circadian clockwork may orchestrate the functionality of the several factors involved in the control of human trophoblast functions that are fundamental for a successfully pregnancy outcome.
Department of Biology and Evolution, University of Ferrara, Via L. Borsari 46, 44100 Ferrara, Italy. laura.lunghi@unife.it
Laura
L
Lunghi
Elena
E
Frigato
Maria Enrica
ME
Ferretti
Carla
C
Biondi
Cristiano
C
Bertolucci
D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D057906:Circadian Clocks / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000502:physiology; D056950:Period Circadian Proteins / Q000502:physiology; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D050777:Stathmin / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014327:Trophoblasts / Q000166:cytology*; D025521:Tumor Suppressor Proteins / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
D018797:Cell Cycle Proteins; C063898:DBP protein, human; C403671:DELEC1 protein, human; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C421902:PER2 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger; C493995:STMN1 protein, human; D050777:Stathmin; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D011505:Protein-Tyrosine Kinases; C499349:WEE1 protein, human