A reconfigurable real-time compressive-sampling camera for biological applications.
Many applications in biology, such as long-term functional imaging of neural and cardiac systems, require continuous high-speed imaging. This is typically not possible, however, using commercially available systems. The frame rate and the recording time of high-speed cameras are limited by the digitization rate and the capacity of on-camera memory. Further restrictions are often imposed by the limited bandwidth of the data link to the host computer. Even if the system bandwidth is not a limiting factor, continuous high-speed acquisition results in very large volumes of data that are difficult to handle, particularly when real-time analysis is required. In response to this issue many cameras allow a predetermined, rectangular region of interest (ROI) to be sampled, however this approach lacks flexibility and is blind to the image region outside of the ROI. We have addressed this problem by building a camera system using a randomly-addressable CMOS sensor. The camera has a low bandwidth, but is able to capture continuous high-speed images of an arbitrarily defined ROI, using most of the available bandwidth, while simultaneously acquiring low-speed, full frame images using the remaining bandwidth. In addition, the camera is able to use the full-frame information to recalculate the positions of targets and update the high-speed ROIs without interrupting acquisition. In this way the camera is capable of imaging moving targets at high-speed while simultaneously imaging the whole frame at a lower speed. We have used this camera system to monitor the heartbeat and blood cell flow of a water flea (Daphnia) at frame rates in excess of 1500 fps.
Institute of Biophysics, Imaging and Optical Science, Schools of Biology and Electrical and Electronic Engineering, The University of Nottingham, Nottingham, United Kingdom.
Bo
B
Fu
Mark C
MC
Pitter
Noah A
NA
Russell
D000818:Animals; D003621:Daphnia / Q000502:physiology; D044962:Data Compression / Q000379:methods*; D006321:Heart / Q000502:physiology; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors