Expression vectors for Acinetobacter baylyi ADP1.
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.
Emory University School of Medicine, Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Atlanta, Georgia, USA.
Charles Daniel
CD
Murin
Kristy
K
Segal
Anton
A
Bryksin
Ichiro
I
Matsumura
D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005966:Glucuronidase