Insulin-like antigen of mangrove leaves and its anti-diabetic activity in alloxan-induced diabetic rats.
The objective of this study was to evaluate the anti-diabetic potential of three mangrove plants, Rhizophora mucronata, Rhizophora apiculata and Rhizophora annamalayana, and to detect the presence of their insulin-like protein. The in vivo anti-diabetic experiment was done on male albino Wister rats. Oral administration of 60 mg kg(-1) leaf powder extract of the three different mangrove plants for 30 days modulated the parameters such as blood glucose, plasma insulin, body weight, total haemoglobin, glycosylated haemoglobin, liver glycogen, plasma and tissue lipids, cholesterol, triglycerides, free fatty acids and phospholipids to normal levels in the alloxan-induced diabetic rats. The anti-diabetic activity of R. apiculata was more pronounced than that of the other mangrove extracts, but it was on a par with the commercial drug glibenclamide. The presence of an insulin-like protein in the mangrove extracts was detected by SDS-PAGE analysis and confirmed through ELISA. Hence, the anti-diabetic activity and the presence of an insulin-like protein in Rhizophora species were proved scientifically.
Center for Advanced Study in Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
Nabeel M
NM
Alikunhi
Kathiresan
K
Kandasamy
Chinthamani
C
Manoharan
Manivannan
M
Subramanian
D000496:Alloxan; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D007328:Insulin / Q000276:immunology*; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000276:immunology*; D051381:Rats
D052179:Antigens, Plant; D007328:Insulin; D010936:Plant Extracts; D000496:Alloxan