Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue.
BACKGROUND
The aim of the study was to assess the performance of Promega, Maxwell® 16 for the extraction of genomic DNA from whole blood and FFPE tissue.


METHODS
DNA was extracted from 10 whole blood and 10 FFPE specimens using six different commercial kits.


RESULTS
For whole blood, the mean DNA concentration obtained by Maxwell® 16 was significantly greater than either easyMAG® (p<0.0001) or QIAamp® Blood DNA kit (p<0.001). For FFPE, the mean DNA concentration obtained by the AllPrep® FFPE specific DNA/RNA kit was significantly greater than either the Maxwell® 16 (p<0.0001) or the general AllPrep® DNA/RNA kit (p<0.0001).


CONCLUSIONS
Comparative evaluation of the six DNA extraction kits indicated that the semi-automated Maxwell® 16 was superior for whole blood extraction while the manual AllPrep® FFPE DNA/RNA kit (Qiagen) performed better for FFPE DNA extraction in terms of quantity of DNA obtained. All six extraction methods (blood and FFPE) performed well in terms of purity. Although there were variances in the quantity of DNA obtained, there were no significant differences in the efficiency of these methods in yielding amplifiable DNA extracts, as demonstrated by β-actin for whole blood specimens. In evaluation of FFPE DNA extraction methods, the Qiagen AllPrep® FFPE DNA/RNA Mini Kit was the best for applications requiring larger amplicons, but for smaller amplicons the Maxwell was most consistent.
Department of Pathology, Children's Medical Center, Dallas, TX, USA. shama.khokhar@childrens.com
Shama Khan
SK
Khokhar
Midori
M
Mitui
Nora Kristine
NK
Leos
Beverly Barton
BB
Rogers
Jason Y
JY
Park
D001331:Automation; D004247:DNA / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction
D005557:Formaldehyde; D004247:DNA