Detection of methicillin-resistant Staphylococcus pseudintermedius with commercially available selective media.
OBJECTIVE
Commercially available selective media for methicillin-resistant Staphylococcus aureus (MRSA) were tested for the detection and isolation of methicillin-resistant Staphylococcus pseudintermedius (MRSP).


RESULTS
Five different screening agars [mannitol salt agar with oxacillin and BD BBL™ Chromagar™ MRSA (BD Diagnostics); chromID™ MRSA agar (bioMérieux); Oxacillin resistance screening agar base (ORSAB); and Brilliance MRSA agar (Oxoid)] were analysed for the detection of MRSP. Bacteria that may be isolated together with MRSP and may grow on the screening agars were included in the study to determine possible interference with the growth of MRSP. MRSP grew well on all selective media except on BD BBL™ Chromagar™ MRSA (BD Diagnostics) and chromID™ MRSA agar (bioMérieux), on which a low to moderate growth rate was noted.


CONCLUSIONS
ORSAB (Oxoid) and Brilliance MRSA agar (Oxoid) are most suitable for the detection and isolation of MRSP from clinical material.


CONCLUSIONS
The importance of MRSP in veterinary medicine is increasing. Diagnostic systems are needed to detect MRSP carrier as soon as possible. This study provides information about selected MRSA screening agars for the detection of MRSP to the clinical microbiologists.
Small Animal Medicine Clinic, LMU Munich, München, Germany.
C
C
Horstmann
R S
RS
Mueller
R K
RK
Straubinger
C
C
Werckenthin
D000362:Agar / Q000737:chemistry; D000818:Animals; D002353:Carrier State; D003470:Culture Media / Q000737:chemistry*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000254:growth & development; D010068:Oxacillin / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D000362:Agar; D010068:Oxacillin