Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol.
α- and β-zearalenol (α-ZOL and β-ZOL, respectively) are metabolites of the mycotoxin zearalenone (ZEN). All three individual mycotoxins have shown to be biological active i.e. being estrogenic and able to stimulate cellular proliferation albeit at different strengths. In this work, cytosol protein expression was determined by using stable-isotope labelling by amino acids in cell culture (SILAC) upon exposure of α-ZOL and β-ZOL to the steroidogenesis cell model H295R. A total of 14 and 5 individual proteins were found to be significantly regulated by α-ZOL and β-ZOL, respectively. Interestingly, there were no common protein regulations by the metabolites or the parent mycotoxin ZEN. Furthermore, the regulated proteins were assigned to networks and groups of actions that also differed from one another suggesting that the three individual mycotoxins may have unique biological activities.
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway.
Øyvind L
ØL
Busk
Caroline
C
Frizzell
Steven
S
Verhaegen
Silvio
S
Uhlig
Lisa
L
Connolly
Erik
E
Ropstad
Morten
M
Sørlie
D002460:Cell Line; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D060066:Protein Interaction Maps; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000378:metabolism; D015025:Zearalenone / Q000737:chemistry / Q000633:toxicity*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity
D011506:Proteins; D013256:Steroids; C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol; C028226:taleranol