Auraptene regulates gene expression involved in lipid metabolism through PPARα activation in diabetic obese mice.
METHODS
Peroxisome proliferator-activated receptor-α (PPARα) is a key regulator of circulating lipid level. Thus, various food-derived compounds that activate PPARα as agonists have been screened and characterized.


RESULTS
We investigated the effects of auraptene, a citrus-derived compound serving as a PPARα agonist in vitro, on abnormalities in lipid and glucose metabolisms. In high-fat-diet (HFD)-fed KK-Ay diabetic obese mice, auraptene treatment suppressed hyperlipidemia and triglyceride accumulation in the liver and skeletal muscle, and increased the mRNA expression levels of the PPARα target genes involved in fatty acid oxidation in the liver and skeletal muscle. Moreover, the adipocyte size in the auraptene-treated mice was significantly smaller than that in the control HFD-fed mice resulting in the improvement of HFD-induced hyperglycemia and abnormalities in glucose tolerance.


CONCLUSIONS
These findings indicate that auraptene activates PPARα also in vivo and its treatment may improve abnormalities in lipid and glucose metabolisms, suggesting that auraptene is a valuable food-derived compound for managing metabolic disorders.
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
Nobuyuki
N
Takahashi
Mari
M
Senda
Shan
S
Lin
Tsuyoshi
T
Goto
Masamichi
M
Yano
Takao
T
Sasaki
Shigeru
S
Murakami
Teruo
T
Kawada
D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D003374:Coumarins / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000503:physiopathology; D004041:Dietary Fats / Q000008:administration & dosage; D005786:Gene Expression Regulation; D006949:Hyperlipidemias / Q000188:drug therapy; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008659:Metabolic Diseases / Q000188:drug therapy; D051379:Mice; D008820:Mice, Obese; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D003374:Coumarins; D004041:Dietary Fats; D047493:PPAR alpha; D014280:Triglycerides; C105832:aurapten