LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis.
A unique method that applied a multilayer-immobilization strategy was developed to prepare nanofibrous enzymes for biosynthesis. LiCl co-electrospun with polyurethane nanofibers enabled strong physical adsorption of bovine serum albumin (BSA), forming the first layer of protein on the nanofibers; lipase AK was subsequently crosslinked to BSA as an outer layer of enzyme. The content of LiCl in nanofibers was found to be a sensitive factor affecting the activity and stability of the immobilized lipase. For biodiesel synthesis from soybean oil and methanol in isooctane, the reaction rate catalyzed by nanofibrious lipase carrying 5 wt% LiCl was 6.6-fold higher than fibers without LiCl, with a conversion of 91% was achieved within 2 h. LiCl also induced much improved enzyme stability. The nanofibrous lipase with 5% LiCl could be repeatedly used for 42 cycles without apparent activity loss, while the immobilized lipase without LiCl lost over 90% activity within 13 reuse cycles.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Chun-Xia
CX
Liu
Song-Ping
SP
Zhang
Zhi-Guo
ZG
Su
Ping
P
Wang
D055162:Biocatalysis / Q000187:drug effects; D056804:Biofuels / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D018021:Lithium Chloride / Q000494:pharmacology*; D057139:Nanofibers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000201:enzymology; D059027:Recycling; D012995:Solubility / Q000187:drug effects; D014867:Water / Q000737:chemistry
D056804:Biofuels; D004800:Enzymes, Immobilized; D011140:Polyurethanes; D014867:Water; D008049:Lipase; D018021:Lithium Chloride