Identification of volatile components in yak butter using SAFE, SDE and HS-SPME-GC/MS.
The volatile components of yak butter were isolated by solvent-assisted flavour evaporation (SAFE), simultaneous distillation extraction (SDE; dichloromethane and diethyl ether as solvent, respectively) and headspace solid-phase microextraction (HS-SPME; CAR/PDMS, PDMS/DVB and DVB/CAR/PDMS fibre extraction, respectively) and were analysed by GC/MS. A total of 83 volatile components were identified under six different conditions, including 28 acids, 12 esters, 11 ketones, 10 lactones, 10 alcohols, 4 other compounds, 2 aldehydes, 2 unsaturated aldehydes, 1 furan, 1 sulphur-containing compound, 1 unsaturated alcohol and 1 unsatruated ketone. Among them, 51 were identified by SAFE, 58 by SDE (45 with dichloromethane as solvent and 41 with diethyl ether as solvent) and 40 by HS-SPME (26 with CAR/PDMS; 26 with PDMS/DVB and 32 with DVB/CAR/PDMS). Three pretreatment methods were compared to show that the volatile components obtained using different methods varied greatly, both in terms of categories and in content. Therefore, a multi-pretreatment method should be adopted, together with GC/MS. A total of 25 aroma-active compounds were detected by gas chromatography-olfactometry, among which 20 aroma-active compounds were found by SDE (14 with dichloromethane as solvent and 14 with diethyl ether as solvent) and 17 by SAFE.
College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi, PR China.
Ning
N
Li
Bao-Guo
BG
Sun
Fu-Ping
FP
Zheng
Hai-Tao
HT
Chen
Si-Yuan
SY
Liu
Chen
C
Gu
Zhen-Yang
ZY
Song
D000818:Animals; D002079:Butter / Q000032:analysis*; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D009812:Odorants; D055549:Volatile Organic Compounds / Q000032:analysis*
D055549:Volatile Organic Compounds; D002079:Butter