Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium, Flavobacterium resistens and Flavobacterium johnsoniae.
A strictly aerobic, Gram-negative, yellow-pigmented, non-spore-forming rod, designated 15C3(T), was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9-33 °C (optimum 25 °C) and pH 5.6-7.9 (optimum pH 6.1-7.0). No growth occurred with >2% (w/v) NaCl. Strain 15C3(T) reduced nitrate to nitrogen and showed catalase activity but not oxidase activity. The predominant fatty acids were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (comprising C(16:1)ω7c and/or iso-C(15:0) 2-OH). The isolate contained phosphatidylethanolamine as the major polar lipid and menaquinone-6 as the major respiratory quinone. The G+C content of the genomic DNA of strain 15C3(T) was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15C3(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium hercynium WB 4.2-33(T) (96.9% sequence similarity). On the basis of phenotypic and phylogenetic distinctiveness, strain 15C3(T) is considered to represent a novel species in the genus Flavobacterium, for which the name Flavobacterium compostarboris sp. nov. is proposed. The type strain is 15C3(T) ( = KACC 14224(T)  = JCM 16527(T)). Emended descriptions of F. hercynium, Flavobacterium resistens and Flavobacterium johnsoniae are also given.
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.|Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Joong-Jae
JJ
Kim
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Eiko
E
Kanaya
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Hang-Yeon
HY
Weon
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Yuichi
Y
Koga
CREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.|Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Kazufumi
K
Takano
Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
Peter F
PF
Dunfield
Korean Agricultural Culture Collection (KACC), National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea.
Soon-Wo
SW
Kwon
Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Shigenori
S
Kanaya
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007564:Japan; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil