Investigation of the relationship between SLA-1 and SLA-3 gene expression and susceptibility to Escherichia coli F18 in post-weaning pigs.
Porcine post-weaning diarrhea and edema disease are principally caused by Escherichia coli strains that produce F18 adhesin. FUT1 genotyping and receptor binding studies divided piglets into E. coli F18-resistant and -sensitive groups, and the roles of SLA-1 and SLA-3 were investigated. SLA-1 and SLA-3 expression was detected in 11 pig tissues, with higher levels of SLA-1 in lung, immune tissues and gastrointestinal tract, and higher levels of SLA-3 also in lung and lymphoid tissues. Both genes were expressed higher in F18-resistant piglets, and their expression was positively correlated in different tissues; a negative correlation was observed in some tissues of F18-sensitive group, particularly in lung and lymphatic samples. Gene ontology and pathway analyses showed that SLA-1 and SLA-3 were involved in 37 biological processes, including nine pathways related to immune functions. These observations help to elucidate the relationship between SLA class I genes and E. coli F18-related porcine gastrointestinal tract diseases.
College of Animal Science and Technology, Yangzhou University, China.
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L
Ye
C
C
Zi
Z Y
ZY
Pan
J
J
Zhu
Z D
ZD
Du
G Q
GQ
Zhu
X G
XG
Huang
W B
WB
Bao
S L
SL
Wu
D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn; D015373:Bacterial Typing Techniques; D004198:Disease Susceptibility / Q000276:immunology; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D005647:Fucosyltransferases / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D007422:Intestines / Q000166:cytology / Q000276:immunology; D008168:Lung / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000378:metabolism; D014886:Weaning; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D018829:Adhesins, Bacterial; D005647:Fucosyltransferases; D015395:Histocompatibility Antigens Class I; D000097631:Galactoside 2-alpha-L-fucosyltransferase