Cloning and characterization of the ζ-carotene desaturase gene from Chlorella protothecoides CS-41.
To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the ζ-carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively. Phylogenetic analysis demonstrated that the ZDS from C. protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species. In addition, we also found that the zds gene expression was upregulated in response to light.
MOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Meiya
M
Li
Zhibing
Z
Gan
Yan
Y
Cui
Chunlei
C
Shi
Xianming
X
Shi
D000595:Amino Acid Sequence; D002338:Carotenoids / Q000096:biosynthesis; D002708:Chlorella / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005786:Gene Expression Regulation / Q000528:radiation effects; D008027:Light; D014975:Lutein / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny
D002338:Carotenoids; D010088:Oxidoreductases; C099215:zeta-carotene desaturase; D014975:Lutein