Salisediminibacterium halotolerans gen. nov., sp. nov., a halophilic bacterium from soda lake sediment.
An orange-pigmented, Gram-reaction-positive, non-spore-forming, halophilic, alkali-tolerant rod, designated strain halo-2(T), was isolated from sediment of Xiarinaoer soda lake, in China's Inner Mongolia Autonomous Region. Strain halo-2(T) grew in a complex medium with 3-30 % (w/v) NaCl and at pH 5-10. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (43.6 %), anteiso-C(17 : 0) (14.8 %) and iso-C(15 : 0) (6.8 %) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of the novel strain was 48.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-2(T) was most closely related to Bacillus agaradhaerens DSM 8721(T) (93.9 % sequence similarity). However, strain halo-2(T) could be clearly differentiated from its closest phylogenetic relatives on the basis of several phenotypic, genotypic and chemotaxonomic characteristics. Strain halo-2(T) therefore represents a novel species in a new genus for which the name Salisediminibacterium halotolerans gen. nov., sp. nov. is proposed. The type strain of the type species is halo-2(T) (= CGMCC 1.7654(T) = NBRC 104935(T)).
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Feng
F
Jiang
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Shu-Juan
SJ
Cao
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Zhao-Hu
ZH
Li
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Hua
H
Fan
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Hai-Feng
HF
Li
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Wei-Jie
WJ
Liu
State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Hong-Li
HL
Yuan
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D006093:Gram-Positive Asporogenous Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S