Viral expression directs the fate of B cells in bovine leukemia virus-infected sheep.
The host immune response is believed to tightly control viral replication of deltaretroviruses such as human T-lymphotropic virus type 1 (HTLV-1) and bovine leukemia virus (BLV). However, this assumption has not been definitely proven in vivo. In order to further evaluate the importance of the immune response in the BLV model, we studied the fate of cells in which viral expression was transiently induced. Using a dual fluorochrome labeling approach, we showed that ex vivo induction of viral expression induces higher death rates of B cells in vivo. Furthermore, cyclosporine treatment of these animals indicated that an efficient immune response is required to control virus-expressing cells.
Cellular and Molecular Biology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Arnaud
A
Florins
Alix
A
de Brogniez
Marjet
M
Elemans
Amel-Baya
AB
Bouzar
Carole
C
François
Michal
M
Reichert
Becca
B
Asquith
Luc
L
Willems
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000821:virology*; D016583:Enzootic Bovine Leukosis / Q000276:immunology / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D012756:Sheep