Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.
Most laboratory assays take advantage of multi-step protocols to achieve high performance, but conventional paper-based tests (e.g., lateral flow tests) are generally limited to assays that can be carried out in a single fluidic step. We have developed two-dimensional paper networks (2DPNs) that use materials from lateral flow tests but reconfigure them to enable programming of multi-step reagent delivery sequences. The 2DPN uses multiple converging fluid inlets to control the arrival time of each fluid to a detection zone or reaction zone, and it requires a method to disconnect each fluid source in a corresponding timed sequence. Here, we present a method that allows programmed disconnection of fluid sources required for multi-step delivery. A 2DPN with legs of different lengths is inserted into a shared buffer well, and the dropping fluid surface disconnects each leg at in a programmable sequence. This approach could enable multi-step laboratory assays to be converted into simple point-of-care devices that have high performance yet remain easy to use.
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. blutz@uw.edu
Barry R
BR
Lutz
Philip
P
Trinh
Cameron
C
Ball
Elain
E
Fu
Paul
P
Yager
D001331:Automation; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010209:Paper; D019095:Point-of-Care Systems; D012996:Solutions / Q000737:chemistry
D012996:Solutions