Immobilization of carbonic anhydrase on spherical SBA-15 for hydration and sequestration of CO2.
Bovine carbonic anhydrase (BCA) was immobilized on spherical SBA-15 through various approaches, including covalent attachment (BCA-CA), adsorption (BCA-ADS), and cross-linked enzyme aggregation (BCA-CLEA). The spherical SBA-15 was characterized by XRD, BET, and FE-SEM analysis. (29)Si CP-MAS NMR was used to confirm the 3-aminopropyltriethoxysilane grafting (an intermediate step in the immobilization technique), and the immobilization of BCA was confirmed by FT-IR spectrum. The catalytic activities for hydration of CO(2) were calculated for free and immobilized BCA with and without buffer. The K(cat) values for free BCA, BCA-CLEA, BCA-CA and BCA-ADS were 0.79, 0.78, 0.58 and 0.36 s(-1), respectively, indicating that BCA-CLEA showed a comparatively higher hydration of CO(2) than BCA-CA and BCA-ADS, which was nearly the same as free BCA. The amount of CaCO(3) precipitated over free BCA, BCA-CLEA, BCA-CA and BCA-ADS were 140, 138, 135 and 130 mg, respectively. Performance studies, including assays on reusability, thermal stability and storage stability, were also carried out for BCA-CLEA. The results confirmed that BCA-CLEA is reusable, thermally stable and, withstands storage, and is thus a suitable candidate for use in hydration and sequestration of CO(2).
Korea Institute of Energy Research, Yuseong-gu, Daejeon, Republic of Korea.
Mari
M
Vinoba
Margandan
M
Bhagiyalakshmi
Soon Kwan
SK
Jeong
Yeo Ii
YI
Yoon
Sung Chan
SC
Nam
D000327:Adsorption; D000818:Animals; D055162:Biocatalysis; D057486:Carbon Cycle; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism; D058572:Carbon Footprint; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017743:Equipment Reuse; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014961:X-Ray Diffraction
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D011437:Propylamines; C509969:SBA-15; D012821:Silanes; D002245:Carbon Dioxide; D012822:Silicon Dioxide; D002256:Carbonic Anhydrases; C477625:amino-propyl-triethoxysilane