Preparation and value assignment of standard reference material 968e fat-soluble vitamins, carotenoids, and cholesterol in human serum.
Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum provides certified values for total retinol, γ- and α-tocopherol, total lutein, total zeaxanthin, total β-cryptoxanthin, total β-carotene, 25-hydroxyvitamin D(3), and cholesterol. Reference and information values are also reported for nine additional compounds including total α-cryptoxanthin, trans- and total lycopene, total α-carotene, trans-β-carotene, and coenzyme Q(10). The certified values for the fat-soluble vitamins and carotenoids in SRM 968e were based on the agreement of results from the means of two liquid chromatographic methods used at the National Institute of Standards and Technology (NIST) and from the median of results of an interlaboratory comparison exercise among institutions that participate in the NIST Micronutrients Measurement Quality Assurance Program. The assigned values for cholesterol and 25-hydroxyvitamin D(3) in the SRM are the means of results obtained using the NIST reference method based upon gas chromatography-isotope dilution mass spectrometry and liquid chromatography-isotope dilution tandem mass spectrometry, respectively. SRM 968e is currently one of two available health-related NIST reference materials with concentration values assigned for selected fat-soluble vitamins, carotenoids, and cholesterol in human serum matrix. This SRM is used extensively by laboratories worldwide primarily to validate methods for determining these analytes in human serum and plasma and for assigning values to in-house control materials. The value assignment of the analytes in this SRM will help support measurement accuracy and traceability for laboratories performing health-related measurements in the clinical and nutritional communities.
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA. jbthomas@nist.gov
Jeanice B
JB
Thomas
David L
DL
Duewer
Isaac O
IO
Mugenya
Karen W
KW
Phinney
Lane C
LC
Sander
Katherine E
KE
Sharpless
Lorna T
LT
Sniegoski
Susan S
SS
Tai
Michael J
MJ
Welch
James H
JH
Yen
D002338:Carotenoids / Q000097:blood* / Q000737:chemistry; D002784:Cholesterol / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D015394:Molecular Structure; D012015:Reference Standards; D014815:Vitamins / Q000097:blood* / Q000737:chemistry
D014815:Vitamins; D002338:Carotenoids; D002784:Cholesterol