Structure and gene cluster of the O-antigen of Escherichia coli O19ab.
The O-polysaccharide (O-antigen) of Escherichia coli O19ab was studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established: [See formula in text] where the degree of O-acetylation of GlcNAc is ∼33%. The O-antigen gene cluster of E. coli O19ab was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O19ab-antigen structure.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
Andrei V
AV
Perepelov
Hongfei
H
Zhu
Sof'ya N
SN
Senchenkova
Quan
Q
Wang
Alexander S
AS
Shashkov
Lei
L
Wang
Yuriy A
YA
Knirel
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics*; D023061:Gene Order; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000235:genetics*
D019081:O Antigens