Simultaneous immunoassay for the detection of two lung cancer markers using functionalized SERS nanoprobes.
A quick and reproducible SERS-based immunoassay, using functionalized hollow gold nanospheres and magnetic beads, has been developed. Here, a simultaneous detection of dual cancer markers in blood serum has been achieved under a single excitation wavelength. The accuracy and sensitivity for clinical sera from five patients confirms their diagnostic feasibility.
Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.
Hyangah
H
Chon
Sangyeop
S
Lee
Soo-Young
SY
Yoon
Soo-Ik
SI
Chang
Dong Woo
DW
Lim
Jaebum
J
Choo
D014408:Biomarkers, Tumor / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000097:blood; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008175:Lung Neoplasms / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins; D006046:Gold