Evaluation of vertical cell fluidity in a multilayered sheet of skeletal myoblasts.
The procedure for fabricating a multilayered cell sheet has been developed by combining multiple sheets using a thermo-responsive surface and stamp system. Confocal laser scanning microscopy revealed that the fluidity of a multilayered sheet of skeletal myoblasts could be estimated as vertical diffusivity and changed upon addition of dermal fibroblasts.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. kino-oka@bio.eng.osaka-u.ac.jp
Masahiro
M
Kino-oka
Trung Xuan
TX
Ngo
Eiji
E
Nagamori
Yasunori
Y
Takezawa
Yasuki
Y
Miyake
Yoshiki
Y
Sawa
Atsuhiro
A
Saito
Tatsuya
T
Shimizu
Teruo
T
Okano
Masahito
M
Taya
D002465:Cell Movement; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D032448:Myoblasts, Skeletal / Q000166:cytology*; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*