Surfaces that sequester serum-borne heparin amplify growth factor activity.
Surfaces presenting a heparin-binding peptide can non-covalently sequester heparin from culture supplements, such as fetal bovine serum. In turn, sequestered, serum-borne heparin can non-covalently localize growth factors at the cell-material interface, resulting in amplified growth factor bioactivity.
Department of Biomedical Engineering, University of Wisconsin-Madison, 53705, USA.
Gregory A
GA
Hudalla
Justin T
JT
Koepsel
William L
WL
Murphy
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006493:Heparin / Q000097:blood* / Q000302:isolation & purification* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties
D010446:Peptide Fragments; D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols; D006493:Heparin