Gold nanoparticles surface-functionalized with paclitaxel drug and biotin receptor as theranostic agents for cancer therapy.
We describe in this study whether the gold nanoparticle (AuNP) surface-functionalized with PEG, biotin, paclitaxel (PTX) and rhodamine B linked beta-cyclodextrin (β-CD) (AuNP-5') can be useful as a theranostic agent for cancer therapy without the cytotoxic effect on normal cells. Prior to surface-functionalizing AuNPs, the cytotoxicity of the nanoparticles was evaluated, followed by their cytocompatibility. PTX, an anti-cancer agent, formed inclusion complexations with β-CD conjugated AuNPs, and effectively released from the AuNP-2' surface-functionalized with PEG, beta-cyclodextrin (β-CD) and paclitaxel (PTX) using the intracellular glutathione (GSH) level (10 mm). Two types of AuNP-4 surface-functionalized with PEG and rhodamine B linked β-CD and AuNP-5 surface-functionalized PEG, biotin and rhodamine B linked β-CD were used for evaluating their specific interaction on cancer cells such as HeLa, A549 and MG63. These were also tested against normal NIH3T3 cell, determining that the AuNP-5 was more effectively involved with the cancer cells. Confocal laser scanning microscopy (CLSM), fluorescence-activated cell-sorting (FACS) and cell viability analyses showed that the AuNP-5' plays a significant role in the diagnosis and therapy of the cancer cells, and may be used in theranostic agents.
Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Dong Nyoung
DN
Heo
Dae Hyeok
DH
Yang
Ho-Jin
HJ
Moon
Jung Bok
JB
Lee
Min Soo
MS
Bae
Sang Cheon
SC
Lee
Won Jun
WJ
Lee
In-Cheol
IC
Sun
Il Keun
IK
Kwon
D000818:Animals; D001710:Biotin / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D017239:Paclitaxel / Q000737:chemistry* / Q000494:pharmacology*; D017978:Receptors, Growth Factor / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
D017978:Receptors, Growth Factor; D047392:beta-Cyclodextrins; C052136:biotin receptor; D001710:Biotin; D006046:Gold; C031215:betadex; D017239:Paclitaxel