Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples.
The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals. The clinical validation showed an increase statistically significant (P<0.05) in the group of diseased animals versus healthy pigs. Therefore, these assays may be used in porcine saliva samples, in addition, the measurement of Igs in saliva could be a practical tool, simple and minimally invasive, to evaluate the humoral immune status of pigs.
Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain.
D
D
Escribano
A M
AM
Gutiérrez
S
S
Martínez Subiela
F
F
Tecles
J J
JJ
Cerón
D000818:Animals; D056724:Immunity, Humoral; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013552:Swine / Q000378:metabolism*; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M