The expression of PEDF and VEGF in the gastric wall of prehepatic portal hypertensive rats.
OBJECTIVE
Upper gastrointestinal bleeding of portal hypertension cases may result from gastric mucosal lesions due to portal hypertensive gastropathy. The pathological changes in the vessels of the gastric wall are very important in the pathogenesis of portal hypertensive gastropathy. However, the mechanisms of these pathological changes are not completely understood. In this study, we examined the expression levels of PEDF and VEGF in the gastric wall in rats with prehepatic portal hypertension.


METHODS
Eighteen healthy Wistar rats were randomly divided into groups A and B. Group A was used to establish the prehepatic portal hypertensive model and group B to evaluate a sham surgery. The VEGF and PEDF expression in the rat gastric wall were detected by immunohistochemical staining and western blotting.


RESULTS
VEGF and PEDF were mainly expressed in the basal layer of the mucosal glands. The expression levels of VEGF and PEDF in group A were higher than that in group B at 7, 10 and 14 days after surgery. The expression levels of VEFG and PEDF in group B did not show significant changes.


CONCLUSIONS
The results from the present study showed a significantly elevated expression of both VEGF and PEDF in the gastric walls during the development of portal hypertension. The expression of these proteins was mainly located in the basal layer of the gastric mucosa.
Department of Hepatobiliary Surgery, Sun Yat-Sen University, Guangzhou, China. panweidong6869@gmail.com
Wei-Dong
WD
Pan
Yanzhang
Y
Liu
Nan
N
Lin
Ruiyun
R
Xu
D000818:Animals; D015153:Blotting, Western; D005136:Eye Proteins / Q000032:analysis*; D005260:Female; D006975:Hypertension, Portal / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015843:Serpins / Q000032:analysis*; D013270:Stomach / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor