Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium.
Salmonellosis outbreaks involving typhoid fever and human gastroenteritis are important diseases in tropical countries where hygienic conditions are often not maintained. A rapid and sensitive method to detect Salmonella spp., Salmonella Typhi and Salmonella Typhimurium is needed to improve control and surveillance of typhoid fever and Salmonella gastroenteritis. Our objective was the concurrent detection and differentiation of these food-borne pathogens using a multiplex PCR. We therefore designed and optimized a multiplex PCR using three specific PCR primer pairs for the simultaneous detection of these pathogens. The concentration of each of the primer pairs, magnesium chloride concentration, and primer annealing temperature were optimized before verification of the specificity of the primer pairs. The target genes produced amplicons at 429 bp, 300 bp and 620 bp which were shown to be 100% specific to each target bacterium, Salmonella spp., Salmonella Typhi and Salmonella Typhimurium, respectively.
Center of Excellence for Food Safety Research, Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Chai Fung
CF
Pui
Woan Chwen
WC
Wong
Lay Ching
LC
Chai
Hai Yen
HY
Lee
Ahmad
A
Noorlis
Tuan Chilek Tuan
TC
Zainazor
John Yew Huat
JY
Tang
Farinazleen Mohamad
FM
Ghazali
Yoke Kqueen
YK
Cheah
Yoshitsugu
Y
Nakaguchi
Mitsuaki
M
Nishibuchi
Son
S
Radu