Periostin-like-factor-induced bone formation within orthopedic maxillary expansion.
OBJECTIVE
To examine whether periostin-like factor (PLF) stimulation of the expanded palatal suture would accelerate mineral formation of bone.


METHODS
Expanded palates of 6-week-old male C57BL/6 mice were maintained in an organ culture system, and tissue was either unstimulated or stimulated with PLF or BMP-7 for 8 days. Bone mineral formation was assessed by Raman microspectroscopy analysis and histological examination.


RESULTS
Raman microspectroscopy analysis demonstrated that unstimulated palates maintained their bone mineral concentration within the palatal suture over 8 days (%increase = 11.29 ± 2.34). In comparison, palates exposed to either PLF (%increase = 29.33 ± 1.61, p = 0.012) or BMP-7 (% increase = 25.49 ± 1.09, p = 0.016) formed significantly more bone at the osteogenic fronts of the palatal suture compared with unstimulated samples at day 8. Alkaline phosphatase activity along the bone edges was markedly greater in the PLF and BMP-7 groups compared with that in unstimulated groups at day 0 and day 8. The levels of osteocalcin proteins in the palatal suture tissues of the PLF and BMP-7 groups were significantly higher than those in the unstimulated group.


CONCLUSIONS
PLF can increase bone mineral formation within the expanded palatal suture.
Department of Orthodontics, Institute of Stomatology, Nanjing Medical University, Nanjing, China.
W-J
WJ
Zhong
W-B
WB
Zhang
J-Q
JQ
Ma
H
H
Wang
Y-C
YC
Pan
L
L
Wang
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001031:Apatites / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D001857:Bone Matrix / Q000187:drug effects; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D003094:Collagen / Q000032:analysis; D003393:Cranial Sutures / Q000187:drug effects* / Q000473:pathology; D017886:Durapatite / Q000032:analysis; D055119:Elastic Modulus; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015591:Microspectrophotometry; D009924:Organ Culture Techniques; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects*; D010155:Palatal Expansion Technique; D010159:Palate / Q000187:drug effects* / Q000473:pathology; D010710:Phosphates / Q000032:analysis; D011897:Random Allocation; D013059:Spectrum Analysis, Raman; D013997:Time Factors
D001031:Apatites; D015415:Biomarkers; D055419:Bone Morphogenetic Protein 7; D015815:Cell Adhesion Molecules; D010710:Phosphates; C525820:bmp7 protein, mouse; C528633:periostin-like factor, mouse; D015675:Osteocalcin; C030782:carboapatite; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase