Adsorption of various antimicrobial agents to endotoxin removal polymyxin-B immobilized fiber (Toraymyxin®).
The presence/absence of adsorption of 9 representative types of antimicrobial agent used in combination with a polymyxin-B immobilized fiber (PMX-F) were determined and the degree of adsorption to PMX-F was quantitatively evaluated. Various antimicrobial agents were dissolved at appropriate concentrations, and PMX-F was added to each solution and incubated at 37°C. Antimicrobial solutions without PMX-F were also similarly incubated as controls. After 2 and 4h, the concentration of each antimicrobial agent was determined using HPLC. To produce an environment closer to the in vivo state, albumin or serum was added, and similar evaluation was performed. In the presence of albumin, the rate of adsorption to PMX-F was relatively high for Cefmetazon(®), Pentcillin(®), Ciproxan(®) and Zyvox(®). In the presence of serum, the adsorption rate was 4.02±2.83% for Pentcillin(®), 5.59±1.00% for Ciproxan(®), and 22.12±3.23% for Zyvox(®). The results of this study suggest that adequate caution is necessary on the clinical use of Zyvox(®), which was adsorbed in the presence of serum as an environment close to the in vivo environment, but the use of other antimicrobial agents in combination with PMX-F may have only slight influences on adsorption to PMX-F.
Department of Pharmaceutical Sciences, Meiji Pharmaceutical University, Tokyo, Japan.
Ken-ichi
K
Shimokawa
Ryotaro
R
Takakuwa
Kumiko
K
Taya
Yuko
Y
Wada
Noriko
N
Yamazaki
Masahiro
M
Murata
Kiyotaka
K
Hirata
Tomohiko
T
Masuno
Hiroyuki
H
Yokota
Fumiyoshi
F
Ishii
D000327:Adsorption; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001424:Bacterial Infections / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004731:Endotoxins / Q000097:blood / Q000737:chemistry*; D006464:Hemoperfusion; D006801:Humans; D011112:Polymyxin B / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D012772:Shock, Septic / Q000097:blood / Q000188:drug therapy* / Q000382:microbiology
D000890:Anti-Infective Agents; D004731:Endotoxins; D011137:Polystyrenes; D012709:Serum Albumin; D011112:Polymyxin B