Microtubule- and dynein-dependent nuclear trafficking of rhesus rhadinovirus in rhesus fibroblasts.
We investigated the role of microtubules in rhesus rhadinovirus (RRV) nuclear trafficking in rhesus fibroblasts. Intact microtubules and microtubule dynamics are required for RRV trafficking to perinuclear regions. RRV trafficking was reduced by an inhibitor of the dynein motor and overexpression of dynamitin. Furthermore, RRV particles are colocalized with microtubules and dynein proteins. These results highlight the important roles of microtubules and dynein-dynactin complexes in the transport of RRV particles to nuclei during primary infection.
Department of Molecular Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.
Wei
W
Zhang
Whitney
W
Greene
Shou-Jiang
SJ
Gao
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology*; D002478:Cells, Cultured; D004398:Dyneins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology*; D006566:Herpesviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D008253:Macaca mulatta; D008870:Microtubules / Q000378:metabolism / Q000821:virology*; D018419:Primate Diseases / Q000378:metabolism / Q000821:virology*; D018144:Rhadinovirus / Q000235:genetics / Q000502:physiology*
D004398:Dyneins