Vasorelaxant effect of isoquinoline derivatives from two species of Popowia perakensis and Phaeanthus crassipetalus on rat aortic artery.
Five bisbenzyl isoquinolines (1-5), three benzyl isoquinolines (6-8), four isoquinoline alkaloids (9-12), and two unclassified compounds (13 and 14) from Popowia perakensis and Phaeanthus crassipetalus were evaluated for their vasorelaxant effect on rat aortic arteries. In aortic rings pre-contracted with phenylephrine (PE, 0.3 μM), some of the bisbenzyl isoquinoline alkaloids, benzyl isoquinoline alkaloids, and isoquinoline alkaloids showed clearly vasorelaxant effects at 30 μM. The action of (-)-limacine (4) was deduced to be mediated through the increased release of NO from endothelial cells, and that of pecrassipine A (7) and backebergine (12) partly mediated by NO release. Further, the action of pecrassipine A (7) and backebergine (12) may be attributed to their inhibition of the voltage-dependent Ca(2+) channel and receptor-operated Ca(2+) channel.
Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan.
Kazumasa
K
Zaima
Yuka
Y
Takeyama
Ikumi
I
Koga
Aiko
A
Saito
Haruka
H
Tamamoto
Saripah Salbiah Syed Abd
SS
Azziz
Mat Ropi
MR
Mukhtar
Khalijah
K
Awang
A Hamid A
AH
Hadi
Hiroshi
H
Morita
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D029061:Annonaceae / Q000737:chemistry*; D001011:Aorta / Q000187:drug effects*; D001158:Arteries / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010656:Phenylephrine / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000737:chemistry / Q000494:pharmacology*
D000470:Alkaloids; D007546:Isoquinolines; D014665:Vasodilator Agents; D010656:Phenylephrine; D009569:Nitric Oxide