Separation and identification of HSP-associated protein complexes from pancreatic cancer cell lines using 2D CN/SDS-PAGE coupled with mass spectrometry.
Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery. A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation. Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure. In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDS-PAGE to separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation. Ten heat-shock-protein- (HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72), and transitional endoplasmic reticulum ATPase (TER ATPase).
Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
Zhiyun
Z
Zhao
Hui
H
Liu
Xinli
X
Wang
Xiaodong
X
Wang
Zhili
Z
Li
D045744:Cell Line, Tumor; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006360:Heat-Shock Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins