Single nucleotide polymorphism marker development in the rubber tree, Hevea brasiliensis (Euphorbiaceae).
OBJECTIVE
We demonstrated the application of high-throughput 454 sequencing technology in the identification of single nucleotide polymorphism (SNP) markers in Hevea brasiliensis.


RESULTS
A total of 5883 putative SNP positions were discovered in silico, and 10 biallelic SNP markers were validated from 454-derived EST sequences. The polymorphism information content (PIC) and the observed heterozygosity (H(o)) ranged from 0.0963-0.5135 and 0.1071-0.4643, respectively.


CONCLUSIONS
These markers can be useful for the construction of genetic maps, the identification of quantitative trait loci linked to commercially desirable traits, and the study of genetic structure in H. brasiliensis.
National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120 Thailand.
Wirulda
W
Pootakham
Juntima
J
Chanprasert
Nukoon
N
Jomchai
Duangjai
D
Sangsrakru
Thippawan
T
Yoocha
Kanikar
K
Therawattanasuk
Sithichoke
S
Tangphatsornruang
D020224:Expressed Sequence Tags; D005819:Genetic Markers; D006579:Heterozygote; D028482:Hevea / Q000235:genetics*; D018895:Microsatellite Repeats; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers