Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine.
TYW1 catalyzes the condensation of N-methylguanosine with two carbon atoms from an unknown second substrate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine found in eukaryal and archaeal tRNA(Phe). Of the potential substrates examined, only incubation with pyruvate resulted in formation of 4-demethylwyosine. Moreover, incubation with C1, C2, C3, or C1,2,3-(13)C-labeled pyruvate showed that C2 and C3 are incorporated while C1 is not. The mechanistic implications of these results are discussed in the context of the structure of TYW1.
Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721-0088, United States.
Anthony P
AP
Young
Vahe
V
Bandarian
D002244:Carbon / Q000737:chemistry; D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D048288:Imidazolines / Q000737:chemistry; D017017:Methanococcus / Q000201:enzymology; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000737:chemistry*; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D012359:tRNA Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
C569459:4-demethylwyosine; D048288:Imidazolines; D009705:Nucleosides; D012360:RNA, Transfer, Phe; D011994:Recombinant Proteins; C467591:wybutosine; D006151:Guanosine; C016578:7-methylguanosine; D002244:Carbon; D019289:Pyruvic Acid; D010088:Oxidoreductases; D012359:tRNA Methyltransferases