Indigo biosynthesis by Comamonas sp. MQ.
An indigo-producing strain was isolated from activated sludge and identified as Comamonas sp. based on 16S rRNA analysis. It produced indigo at 26.5 mg/l with a conversion of indole to indigo of 47%. Indole at 50 mg/l plus 200 mg naphthalene/l gave 32.2 mg indigo/l with a 58% conversion. A pathway for indigo formation is proposed. This is the first study of indigo biosynthesis by Comamonas sp.
State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
Yuanyuan
Y
Qu
Xuwang
X
Zhang
Qiao
Q
Ma
Fang
F
Ma
Qiang
Q
Zhang
Xinliang
X
Li
Hao
H
Zhou
Jiti
J
Zhou
D053898:Biosynthetic Pathways / Q000235:genetics; D016000:Cluster Analysis; D020585:Comamonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007203:Indigo Carmine; D007211:Indoles / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007211:Indoles; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D007203:Indigo Carmine