Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission.
Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.
Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.
Arijit
A
Kumar De
Debjit
D
Roy
Debabrata
D
Goswami
D005456:Fluorescent Dyes; D007211:Indoles; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055620:Optical Phenomena; D013997:Time Factors; D014966:Xanthenes
D005456:Fluorescent Dyes; D007211:Indoles; D014966:Xanthenes; C007293:DAPI; C034657:Texas red