Tanshinone IIA and tanshinone I production by Trichoderma atroviride D16, an endophytic fungus in Salvia miltiorrhiza.
In this study the isolation of an endophytic fungus from the root of the medicinal herb Salvia miltiorrhiza Bunge is reported for the first time. The fungus produced tanshinone I and tanshinone IIA in rich mycological medium (potato dextrose broth) under shake flask and bench scale fermentation conditions. The fungus was identified as Trichoderma atroviride by its morphology and authenticated by ITS analysis (ITS1 and ITS2 regions and the intervening 5.8S rDNA region). Tanshinone I and tanshinone IIA were identified by HPLC and LC-HRMS/MS and confirmed through comparison with authentic standards. This endophytic fungus has significant scientific and industrial potential to meet the pharmaceutical demands for tanshinone I and tanshinone IIA in a cost-effective, easily accessible and reproducible way.
Department of Pharmacognosy School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Qianliang
Q
Ming
Ting
T
Han
Wenchao
W
Li
Qiaoyan
Q
Zhang
Hong
H
Zhang
Chengjian
C
Zheng
Fang
F
Huang
Khalid
K
Rahman
Luping
L
Qin
D045784:Abietanes / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D060026:Endophytes / Q000737:chemistry / Q000254:growth & development; D005285:Fermentation; D000432:Methanol / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry / Q000382:microbiology; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000382:microbiology*; D014242:Trichoderma / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
D045784:Abietanes; D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal; D012340:RNA, Ribosomal, 5.8S; C021751:tanshinone; D000432:Methanol