Expression of pattern recognition receptors in liver biopsy specimens of children chronically infected with HBV and HCV.
Pattern recognition receptors (PRRs) constitute a pivotal arm of innate immunity. Their distribution is widespread and not limited to cells of the immune system. Following our previous findings concerning the expression of Toll-like receptors (TLRs) 2, 3 and 4 in chronic viral hepatitis C of children, we wished to search for other PRRs, including other TLRs, NOD-like receptors (NLRs) and RIG-1-like helicase receptors (RLR) in infected hepatocytes. Liver biopsy fragments from ten children with chronic hepatitis B and C were used and two others in which hepatotropic virus infection was excluded. Frozen sections of liver samples were subjected to ABC immunohistochemistry (IHC) following incubation with a set of antibodies. Results of IHC findings were screened for correlation with clinical/laboratory data of patients. It was found that several PRRs could be shown in affected hepatocytes, but the incidence was higher in hepatitis C than in B. In hepatitis C, TLR1, 2, 4, NALP and RIG-1 helicase showed the most marked expression. In hepatitis B, TLR1, 3, 9, NOD1 and NALP expression were the most conspicuous. Expression PRRs in liver from hepatitis of unknown origin was much lower. It was also the case in cytospins from human hepatoma cell line. Several correlations between PRRs expression and clinical findings in patients could be shown by statistical exploration. In conclusion, this data suggests some role for PRRs in the pathogenesis of chronic viral hepatitis.
Chair and Department of Infectious Diseases, University of Medical Sciences, Poznan, Poland.
Iwona
I
Mozer-Lisewska
Arleta
A
Kowala-Piaskowska
Anna
A
Mania
Renata
R
Jenek
Husam
H
Samara
Elżbieta
E
Kaczmarek
Jan
J
Sikora
Wojciech
W
Służewski
Jan
J
Zeromski
D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology; D000293:Adolescent; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000276:immunology; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002648:Child; D016174:Hepacivirus / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000097:blood / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000473:pathology / Q000821:virology; D008113:Liver Neoplasms; D008297:Male; D000070576:NLR Proteins; D053474:Nod1 Signaling Adaptor Protein / Q000276:immunology; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000276:immunology*; D051193:Toll-Like Receptors / Q000276:immunology
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D000070576:NLR Proteins; C427903:NLRP1 protein, human; C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein; D051192:Receptors, Pattern Recognition; D051193:Toll-Like Receptors