Classification of lung cancer histology by gold nanoparticle sensors.
We propose a nanomedical device for the classification of lung cancer (LC) histology. The device profiles volatile organic compounds (VOCs) in the headspace of (subtypes of) LC cells, using gold nanoparticle (GNP) sensors that are suitable for detecting LC-specific patterns of VOC profiles, as determined by gas chromatography-mass spectrometry analysis. Analyzing the GNP sensing signals by support vector machine allowed significant discrimination between (i) LC and healthy cells; (ii) small cell LC and non-small cell LC; and between (iii) two subtypes of non-small cell LC: adenocarcinoma and squamous cell carcinoma. The discriminative power of the GNP sensors was then linked with the chemical nature and composition of the headspace VOCs of each LC state. These proof-of-concept findings could totally revolutionize LC screening and diagnosis, and might eventually allow early and differential diagnosis of LC subtypes with detectable or unreachable lung nodules.


BACKGROUND
In this study, a nanomedical device that profiles volatile organic compounds (VOCs) in lung cancer cells is investigated, using a matrix of gold nanoparticle (GNP) sensors that are suitable for detecting lung cancer (LC) specific patterns of VOC profiles. This device might eventually allow early differential diagnosis of LC subtypes including unreachable lung nodules.
The Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa, Israel.
Orna
O
Barash
Nir
N
Peled
Ulrike
U
Tisch
Paul A
PA
Bunn
Fred R
FR
Hirsch
Hossam
H
Haick
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D055088:Early Detection of Cancer; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000145:classification / Q000175:diagnosis / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000737:chemistry
D055549:Volatile Organic Compounds; D006046:Gold