Clinical isolates of Vibrio fluvialis from Kolkata, India, obtained during 2006: plasmids, the qnr gene and a mutation in gyrase A as mechanisms of multidrug resistance.
Resistance profiles and their correlation with genetic factors were investigated in 12 isolates of Vibrio fluvialis obtained from hospitalized patients in Kolkata, India, in 2006. All the strains displayed drug resistance with varying antibiograms. However, resistance to ampicillin and neomycin was common to all of them. Three isolates harboured plasmids carrying drug-resistance genes that could be transferred to recipient strains by conjugation and transformation. PCR results indicated the absence of class 1 integrons and SXT elements in these isolates. A mutation in gyrase A (serine 83→isoleucine) and the presence of the qnrVC-like [corrected] gene were found to contribute towards quinolone resistance. In the 12 isolates, the qnrVC-like [corrected] gene was associated only with two plasmid-bearing isolates, L10734 and L9978, which displayed resistance to quinolones. The gene was transferable during transformation and conjugation, indicating that it was plasmid-borne. Taken together, these data indicate that plasmids, the qnrVC-like [corrected] gene and a mutation in gyrase A were responsible for the observed drug resistance in these strains. To the best of our knowledge, this is the first report of the presence of the qnrVC-like [corrected] allele in V. fluvialis isolates from India.
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Rochika
R
Singh
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Neha
N
Rajpara
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Jyoti
J
Tak
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Arati
A
Patel
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Priyabrata
P
Mohanty
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Kittappa
K
Vinothkumar
National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata, India.
Goutam
G
Chowdhury
National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata, India.
Thandavarayan
T
Ramamurthy
National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata, India.
Amit
A
Ghosh
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar, Gujarat, India.
Ashima Kushwaha
AK
Bhardwaj
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002771:Cholera / Q000382:microbiology; D003227:Conjugation, Genetic; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D022761:Gene Transfer, Horizontal; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010957:Plasmids / Q000032:analysis*; D015363:Quinolones / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000235:genetics* / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones; D027081:DNA Gyrase