Assessing antibody microarrays for space missions: effect of long-term storage, gamma radiation, and temperature shifts on printed and fluorescently labeled antibodies.
Antibody microarrays are becoming frequently used tools for analytical purposes. A key factor for optimal performance is the stability of the immobilized (capturing) antibodies as well as those that have been fluorescently labeled to achieve the immunological test (tracers). This is especially critical for long-distance transport, field testing, or planetary exploration. A number of different environmental stresses may affect the antibody integrity, such as dryness, sudden temperature shift cycles, or, as in the case of space science, exposure to large quantities of the highly penetrating gamma radiation. Here, we report on the effect of certain stabilizing solutions for long-term storage of printed antibody microarrays under different conditions. We tested the effect of gamma radiation on printed and freeze- or vacuum-dried fluorescent antibodies at working concentrations (tracer antibodies), as well as the effect of multiple cycles of sudden and prolonged temperature shifts on the stability of fluorescently labeled tracer antibody cocktails. Our results show that (i) antibody microarrays are stable at room temperature when printed on stabilizing spotting solutions for at least 6 months, (ii) lyophilized and vacuum-dried fluorescently labeled tracer antibodies are stable for more than 9 months of sudden temperature shift cycles (-20°C to 25°C and 50°C), and (iii) both printed and freeze- or vacuum-dried fluorescent tracer antibodies are stable after several-fold excess of the dose of gamma radiation expected during a mission to Mars. Although different antibodies may exhibit different susceptibilities, we conclude that, in general, antibodies are suitable for use in planetary exploration purposes if they are properly treated and stored with the use of stabilizing substances.
Department of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Madrid, Spain.
Graciela
G
de Diego-Castilla
Patricia
P
Cruz-Gil
Eva
E
Mateo-Martí
Patricia
P
Fernández-Calvo
Luis A
LA
Rivas
Víctor
V
Parro
D000906:Antibodies / Q000032:analysis* / Q000528:radiation effects; D055910:Antibodies, Immobilized / Q000032:analysis / Q000528:radiation effects; D005456:Fluorescent Dyes; D005612:Freeze Drying; D005720:Gamma Rays; D040081:Protein Array Analysis; D012996:Solutions; D013026:Space Flight; D013696:Temperature
D000906:Antibodies; D055910:Antibodies, Immobilized; D005456:Fluorescent Dyes; D012996:Solutions