Conformation specificity and arene binding in a peptide composed only of Lys, Ile, Ala and Gly.
The first life on Earth is believed to have been based on RNA, but might have taken advantage of amino acids and short peptides which form readily under conditions like those of the primitive Earth. We have shown that simple peptides adopt specifically folded four-helix bundle structures that can recognize and cleave RNA. Here, to explore the limits of conformational specificity, we characterize a simpler peptide composed of just Lys, Ile, Ala, and Gly called KIA7I. Using nuclear magnetic resonance (NMR) spectroscopy and molecular dynamics (MD) simulations, we find kinks in the helices of KIA7I and multiple C-terminal conformations. These results suggest that the C-terminal Ile residue does not completely occupy the hydrophobic pocket that is filled by aromatic side-chains in well-folded KIA7 variants. The capacity of arenes to fill this cavity was tested. Using NMR, we show that benzene and phenol can bind KIA7I, but do not bind the well-folded variant KIA7W or hen egg white lysozyme. Benzene also binds Aβ(1-40), a mostly disordered polypeptide implicated in Alzheimer's disease. 8-Anilinonaphthalene-1-sulfonate (ANS) fluorescence is further enhanced in the presence of both KIA7I and arenes relative to KIA7I alone. This ANS fluorescence enhancement is stronger for smaller and less polar arenes and less ordered KIA variants. These results suggest that arenes are not confined to the pocket, but penetrate and loosen the hydrophobic core of KIA7I.
Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, Madrid, Spain.
Fernando
F
Diez-García
Irene
I
Gómez-Pinto
Avijit
A
Chakrabartty
Carlos
C
González
Douglas V
DV
Laurents
D000409:Alanine; D000596:Amino Acids; D001554:Benzene / Q000378:metabolism*; D005998:Glycine; D007532:Isoleucine; D008239:Lysine; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D019800:Phenol / Q000378:metabolism*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D010455:Peptides; D007532:Isoleucine; D019800:Phenol; D001554:Benzene; D008239:Lysine; D000409:Alanine; D005998:Glycine