Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix.
OBJECTIVE
To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel's diverticulum and appendicitis.


METHODS
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel's diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR.


RESULTS
Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel's diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes.


CONCLUSIONS
Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel's diverticulum or in acute appendicitis.
Department of Medical Microbiology, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden. perenbaglan@yahoo.com
Peren H
PH
Karagin
Unne
U
Stenram
Torkel
T
Wadström
Asa
A
Ljungh
D000293:Adolescent; D000328:Adult; D000368:Aged; D001064:Appendicitis / Q000382:microbiology* / Q000473:pathology; D001065:Appendix / Q000382:microbiology* / Q000473:pathology; D001439:Bacteroides / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D004754:Enterobacter / Q000235:genetics; D005260:Female; D016998:Helicobacter / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008467:Meckel Diverticulum / Q000382:microbiology* / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D055815:Young Adult
D004269:DNA, Bacterial