Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds.
Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds.
Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
Desirée Cigaran
DC
Schuck
Ramira Yuri
RY
Ribeiro
Arthur A
AA
Nery
Henning
H
Ulrich
Célia R S
CR
Garcia
D000818:Animals; D001583:Benzoxazoles / Q000032:analysis; D002453:Cell Cycle; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology* / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D008018:Life Cycle Stages / Q000502:physiology; D008288:Malaria / Q000469:parasitology* / Q000473:pathology; D008550:Melatonin / Q000494:pharmacology; D016788:Plasmodium chabaudi / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010963:Plasmodium falciparum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011806:Quinolinium Compounds / Q000032:analysis; D012701:Serotonin / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods*; D014363:Tryptamines / Q000494:pharmacology
D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; D014363:Tryptamines; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D012701:Serotonin; C030820:tryptamine; D008550:Melatonin