In vivo low-coherence spectroscopic measurements of local hemoglobin absorption spectra in human skin.
Localized spectroscopic measurements of optical properties are invaluable for diagnostic applications that involve layered tissue structures, but conventional spectroscopic techniques lack exact control over the size and depth of the probed tissue volume. We show that low-coherence spectroscopy (LCS) overcomes these limitations by measuring local attenuation and absorption coefficient spectra in layered phantoms. In addition, we demonstrate the first in vivo LCS measurements of the human epidermis and dermis only. From the measured absorption in two distinct regions of the dermal microcirculation, we determine total hemoglobin concentration (3.0±0.5 g∕l and 7.8±1.2 g∕l) and oxygen saturation.
University of Amsterdam, Academic Medical Center, Department of Biomedical Engineering and Physics, P.O. Box 22700, NL-1100 DE Amsterdam, The Netherlands. n.bosschaart@amc.uva.nl
Nienke
N
Bosschaart
Dirk J
DJ
Faber
Ton G
TG
van Leeuwen
Maurice C G
MC
Aalders
D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008833:Microcirculation; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D012867:Skin / Q000098:blood supply* / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
D006454:Hemoglobins