Studies on the molecular correlates of genomic stability in rat brain cells following Amalakirasayana therapy.
Adult Wistar NIN (WNIN) rats (6 months old) of both sexes were orally fed Amalakirasayana at a dose of 4.5 g per kg body weight, five days in a week. The Amalakirasayana was prepared by Arya Vaidya Sala, Kottakkal, Kerala, India, which is considered as gold standard. After 3, 9 and 15 months of such therapeutic regime, rats were sacrificed and various tissues including brain were removed. Isolated cell suspensions of neurons and astroglia were prepared from the cerebral cortex. DNA damage, as a prime indicator of the status of genomic stability was measured in terms of single (SSBs) and double strand breaks (DSBs) through (a). The "comet" assay and (b). The biochemical methods utilizing the unique properties of Escherichia coli DNA polymerase I (pol I) and calf thymus terminal transferase. The results convincingly indicate that while in control animals, there was a distinct increase in DNA damage with age in neurons and astrocytes, rasayana fed animals showed significantly less DNA damage in brain cells demonstrating beneficial effects of Rasayana therapy towards maintenance of genomic stability. DNA-damage may be the proximal cause of aging and strategies to reduce the rate of aging could be based on this fact.
Centre for Innovative Research, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad-500 085, Andhra Pradesh, India.
Umakanta
U
Swain
Kiran Kumar
KK
Sindhu
Ushasri
U
Boda
Suresh
S
Pothani
Nappan V
NV
Giridharan
Manchala
M
Raghunath
Kalluri Subba
KS
Rao
D000284:Administration, Oral; D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology; D020552:Comet Assay; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects*; D053904:DNA Breaks, Single-Stranded / Q000187:drug effects*; D005260:Female; D042822:Genomic Instability / Q000187:drug effects*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
C574902:Amalakirasayana; D010936:Plant Extracts