VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity.
Vibrio parahaemolyticus, a Gram-negative halophilic bacterium that causes acute gastroenteritis in humans, is characterized by two type III secretion systems (T3SS), namely T3SS1 and T3SS2. T3SS2 is indispensable for enterotoxicity but the effector(s) involved are unknown. Here, we identify VopV as a critical effector that is required to mediate V. parahaemolyticus T3SS2-dependent enterotoxicity. VopV was found to possess multiple F-actin-binding domains and the enterotoxicity caused by VopV correlated with its F-actin-binding activity. Furthermore, a T3SS2-related secretion system and a vopV homologous gene were also involved in the enterotoxicity of a non-O1/non-O139 V. cholerae strain. These results indicate that the F-actin-targeting effector VopV is involved in enterotoxic activity of T3SS2-possessing bacterial pathogens.
Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hirotaka
H
Hiyoshi
Toshio
T
Kodama
Kazunobu
K
Saito
Kazuyoshi
K
Gotoh
Shigeaki
S
Matsuda
Yukihiro
Y
Akeda
Takeshi
T
Honda
Tetsuya
T
Iida
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D006651:Histocytochemistry; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D008853:Microscopy; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011817:Rabbits; D014734:Vibrio cholerae / Q000472:pathogenicity; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D001426:Bacterial Proteins; D037521:Virulence Factors