Administration of Lactobacillus fermentum CRL1446 increases intestinal feruloyl esterase activity in mice.
OBJECTIVE
To evaluate the effect of oral administration of Lactobacillus fermentum CRL1446 on the intestinal feruloyl esterase (FE) activity and oxidative status of mice.


RESULTS
Adult Swiss albino mice received Lact. fermentum CRL1446 at the doses 10(7) and 10(9) cells per day per mouse for 2, 5, 7 and 10 days. Intestinal FE activity, intestinal microbiota counts, plasmatic thiobarbituric acid-reactive substances (TBARS) percentage and glutathione reductase (GR) activity were determined. Mice that received Lact. fermentum CRL1446 at the dose 10(7) cells per day for 7 days showed a twofold increase in total intestinal FE activity, compared to the nontreated group. In large intestine content, FE activity increased up to 6·4 times. No major quantitative changes in colonic microbiota were observed in treated animals. Administration of this strain produced an approx. 30-40% decrease in the basal levels of plasmatic TBARS and an approx. twofold increase in GR activity from day 5 of feeding with both doses.


CONCLUSIONS
Oral administration of Lact. fermentum CRL1446 to mice increases total intestinal FE activity, decreases the basal percentage of plasmatic lipoperoxides and increases GR activity.


CONCLUSIONS
Lactobacillus fermentum CRL1446 could be orally administered as a dietary supplement or functional food for increasing the intestinal FE activity to enhance the bioavailability of ferulic acid, thus improving oxidative status.
Centro de Referencia para Lactobacilos-Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Argentina.
M C Abeijón
MC
Mukdsi
M P Gauffin
MP
Cano
S N
SN
González
R B
RB
Medina
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000378:metabolism; D007420:Intestine, Large / Q000201:enzymology / Q000382:microbiology; D007421:Intestine, Small / Q000201:enzymology* / Q000378:metabolism; D052197:Limosilactobacillus fermentum; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D019936:Probiotics / Q000008:administration & dosage*
D003373:Coumaric Acids; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase