Comparison of real-time PCR and a microimmunofluorescence serological assay for detection of chlamydophila pneumoniae infection in an outbreak investigation.
We assessed the performance of a recently validated real-time PCR assay and a commercially available microimmunofluorescence serologic test for the detection of Chlamydophila pneumoniae infection during an outbreak. Evaluation of specimens from 137 individuals suggests that real-time PCR holds greater utility as a diagnostic tool for early C. pneumoniae detection.
Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Alvaro J
AJ
Benitez
Kathleen A
KA
Thurman
Maureen H
MH
Diaz
Laura
L
Conklin
Newton E
NE
Kendig
Jonas M
JM
Winchell
D023521:Chlamydophila Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004196:Disease Outbreaks; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity