Paternal treatment with cisplatin impairs reproduction of adult male offspring in rats.
This study evaluated the acute and persistent effects of paternal cisplatin-treatment on progeny. Wistar male rats (45 days old) were assigned into 2 groups. Control and cisplatin (CP: 1mg/kg-d, 5 days/week, for 3 weeks, ip.). Male rats at 66 (end of treatment, acute effects evaluation) and 140 days old (after recovery period, persistent effects evaluation) were mated with females. Fetal and post-natal developments of the offspring sired by treated-male mated in both ages were evaluated, including fertility of adult male offspring. No adverse effects in fetal development or puberty onset were seen in CP offspring. However, testicular descent was delayed and postnatal growth was impaired in these animals (acute effect). Moreover, seminal vesicle weight and epididymal sperm count from adult progeny were affected (acute effects) by paternal CP-exposure. The only persistent effect found was alterations in the spermatogenesis. We conclude that paternal CP-administration during peri-puberty affects reproductive endpoints of the progeny.
Program of Cellular and Structural Biology, State University of Campinas, Campinas, SP, Brazil.
Ana Paula Alves
AP
Favareto
Fabíola Choqueta
FC
de Toledo
Wilma De Grava
Wde G
Kempinas
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000008:administration & dosage / Q000633:toxicity*; D004822:Epididymis / Q000166:cytology; D005260:Female; D005298:Fertility / Q000187:drug effects; D047109:Fetal Development / Q000187:drug effects; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018812:Paternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D012669:Seminal Vesicles / Q000473:pathology; D012741:Sexual Maturation / Q000187:drug effects; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000254:growth & development
D000970:Antineoplastic Agents; D002945:Cisplatin