Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction.
This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil. anahi_sv@yahoo.com.br
Anahí Souto
AS
Vieira
Grácia Maria Soares
GM
Rosinha
Carina Elisei de
CE
Oliveira
Silvio Arruda
SA
Vasconcellos
Paulo Andre
PA
Lima-Borges
Walfrido Moraes
WM
Tomás
Guilherme Miranda
GM
Mourão
Ana Cristina Reis
AC
Lacerda
Cleber Oliveira
CO
Soares
Flábio Ribeiro de
FR
Araújo
Ubiratan
U
Piovezan
Carlos Andre
CA
Zucco
Aiesca Oliveira
AO
Pellegrin
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D003670:Deer / Q000382:microbiology*; D005260:Female; D007921:Leptospira interrogans / Q000276:immunology / Q000302:isolation & purification*; D041401:Leptospira interrogans serovar pomona / Q000276:immunology / Q000302:isolation & purification; D007922:Leptospirosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons; D053833:Wetlands
D000907:Antibodies, Bacterial