Parasitism by protozoan Ichthyophthirius multifiliis enhanced invasion of Aeromonas hydrophila in tissues of channel catfish.
Protozoan Ichthyophthirius multifiliis Fouquet (Ich) and bacterium Aeromonas hydrophila are two common pathogens of cultured fish, which cause high fish mortality. Currently there is no information available for the effect of parasitism by Ich on survival of channel catfish and invasion of A. hydrophila in fish tissues following exposure to A. hydrophila. A trial was conducted in this study to: (1) determine whether A. hydrophila increased fish mortality in Ich-parasitized channel catfish; and (2) compare the bacterial quantity in different tissues between non-parasitized and Ich-parasitized catfish by real-time polymerase chain reaction (qPCR). The results demonstrated that the Ich-parasitized catfish showed significantly (P<0.05) higher mortality (80%) when exposed to A. hydrophila by immersion than non-parasitized fish (22%). Low mortality was observed in catfish exposed to Ich alone (35%) or A. hydrophila alone (22%). A. hydrophila in fish tissues were quantified by qPCR using a pair of gene-specific primers and reported as genome equivalents per mg of tissue (GEs/mg). Skin, gill, kidney, liver and spleen in Ich-parasitized fish showed significantly higher load of A. hydrophila (9400-188,300 GEs/mg) than non-parasitized fish (4700-42,100 GEs/mg) after exposure to A. hydrophila. This study provides evidence that parasite infections enhance bacterial invasion and cause high fish mortality.
U.S. Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Laboratory, 990 Wire Road, Auburn, AL 36832, USA. dehai.xu@ars.usda.gov
De-Hai
DH
Xu
Julia W
JW
Pridgeon
Phillip H
PH
Klesius
Craig A
CA
Shoemaker
D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D058491:Bacterial Load; D016770:Ciliophora Infections / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D005393:Fish Diseases / Q000382:microbiology* / Q000401:mortality / Q000469:parasitology*; D005880:Gills / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D016803:Hymenostomatida / Q000502:physiology; D007059:Ictaluridae / Q000382:microbiology* / Q000469:parasitology*; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012867:Skin / Q000382:microbiology