[Distribution of Apo E gene sequencing in patients with coronary heart disease of phlegm-stasis syndrome].
OBJECTIVE
To study the correlation between Apo E gene polymorphism and patients with coronary heart disease (CHD) of phlegm-stasis syndrome (PSS).


METHODS
78 CHD patients were assigned to PSS (49 cases) and non-phlegm-stasis syndrome (NPSS). Polymorphisms of Apo E gene in 78 CHD patients and 100 healthy subjects were detected by complete DNA sequencing.


RESULTS
Five gene types as E3/3, E4/4, E2/ 3, E2/4, and E3/4 were detected in the two groups. The frequencies of genotype E3/3 and epsilon 3 allele were significantly lower in CHD patients than in the healthy subjects (P<0.01). But the frequencies of genotype E3/4 and epsilon 4 allele were significantly higher in CHD patients than in the healthy subjects (P<0.01). In CHD patients, the frequencies of genotype E2/4 + E3/4 + E4/4 and epsilon 4 allele were higher in PSS than in NPSS.


CONCLUSIONS
Apo E epsilon 4 allele was a susceptible allele to CHD, which was closely correlated to CHD PSS. It was inferred that it might be one of main susceptible alleles for CHD PSS.
Department of Cardiology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029. jsfy@jsmail.com.cn
Zhu-yuan
ZY
Fang
Wei-min
WM
Jiang
Chang-le
CL
Zhu
D000368:Aged; D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D003327:Coronary Disease / Q000175:diagnosis* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged
D001057:Apolipoproteins E