Functional improvement of porcine neonatal pancreatic cell clusters via conformal encapsulation using an air-driven encapsulator.
Transplantation of islet cells into diabetic patients is a promising therapy, provided that the islet cells are able to evade host immune rejection. With improved islet viability, this strategy may effectively reverse diabetes. We applied 2% calcium alginate to generate small and large capsules to encapsulate porcine neonatal pancreatic cell clusters (NPCCs) using an air-driven encapsulator. After encapsulation, the viability was assessed at 1, 4, 7, 14 and 28 days and secretion of functional insulin in response to glucose stimulation were tested at days 14 and 28. Selective permeability of the small alginate capsules was confirmed using various sizes of isothiocyanate-labeled dextran (FITC-dextran). Encapsulation of NPCCs was performed without islet protrusion in the small and large capsules. The viability of NPCCs in all experimental groups was greater than 90% at day 1 and then gradually decreased after day 7. The NPCCs encapsulated in large capsules showed significantly lower viability (79.50 ± 2.88%) than that of na&iuml;ve NPCCs and NPCCs in small capsule (86.83 ± 2.32%, 87.67 ± 2.07%, respectively) at day 7. The viability of na&iuml;ve NPCCs decreased rapidly at day 14 (75.67 ± 1.75%), whereas the NPCCs encapsulated in small capsules maintained (82.0 ± 2.19%). After 14 and 28 days NPCCs' function in small capsules (2.67 ± 0.09 and 2.13 ± 0.09) was conserved better compared to that of na&iuml;ve NPCCs (2.04 ± 0.25 and 1.53 ± 0.32, respectively) and NPCCs in large capsules (2.04 ± 0.34 and 1.13 ± 0.10, respectively), as assessed by a stimulation index. The small capsules also demonstrated selective permeability. With this encapsulation technique, small capsules improved the viability and insulin secretion of NPCCs without islet protrusion.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul, Korea.
Sol Ji
SJ
Park
Soojeong
S
Shin
Ok Jae
OJ
Koo
Joon Ho
JH
Moon
Goo
G
Jang
Curie
C
Ahn
Byeong Chun
BC
Lee
Young Je
YJ
Yoo
D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002214:Capsules / Q000737:chemistry; D002470:Cell Survival; D003920:Diabetes Mellitus / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006084:Graft Rejection / Q000209:etiology / Q000517:prevention & control*; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism* / Q000473:pathology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D011183:Postoperative Complications / Q000209:etiology / Q000517:prevention & control*; D013552:Swine
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D007328:Insulin; D020723:Glucuronic Acid