Effect Of G2706A and G1051A polymorphisms of the ABCA1 gene on the lipid, oxidative stress and homocystein levels in Turkish patients with polycystıc ovary syndrome.
BACKGROUND
Obesity, insulin resistance and hyperandrogenism, crucial parameters of Polycystic ovary syndrome (PCOS) play significant pathophysiological roles in lipidemic aberrations associated within the syndrome. Parts of the metabolic syndrome (low HDL and insulin resistance) appeared to facilitate the association between PCOS and coronary artery disease, independently of obesity. ABCA1 gene polymorphism may be altered this components in PCOS patients.In this study, we studied 98 PCOS patients and 93 healthy controls. All subjects underwent venous blood drawing for complete hormonal assays, lipid profile, glucose, insulin, malondialdehyde, nitric oxide, disulfide levels and ABCA genetic study.


RESULTS
In PCOS group fasting glucose, DHEAS, 17-OHP, free testosterone, total-cholesterol, triglyceride, LDL-cholesterol and fibrinogen were significantly different compare to controls. The genotype ABCA G2706A distribution differed between the control group (GG 60.7%, GA 32.1%, AA 7.1%) and the PCOS patients (GG 8.7%, GA 8.7%, AA 76.8%). The frequency of the A allele (ABCAG2706A) was higher in PCOS patients than control group with 13,0% and 23,2%, respectively. In this study, the homocystein and insulin levels were significantly higher in PCOS patients with ABCA G1051A mutant genotype than those with heterozygote and wild genotypes.


CONCLUSIONS
We found higher percentage of AA genotype and A allele of ABCA G2706A in PCOS patients compare to controls. The fasting insulin and homocystein levels were significantly higher in PCOS patients with ABCA G1051A mutant genotype than those with heterozygote and wild genotypes.
Department of Endocrinology, Sifa University, Health Application and Research Center, Izmir, Turkey. muammermd@hotmail.com.
Muammer
M
Karadeniz
Mehmet
M
Erdoğan
Zengi
Z
Ayhan
Murat
M
Yalcin
Murat
M
Olukman
Sevki
S
Cetinkalp
Gulinnaz E
GE
Alper
Zuhal
Z
Eroglu
Asli
A
Tetik
Vildan
V
Cetintas
Ahmet G
AG
Ozgen
Fusun
F
Saygili
Candeger
C
Yilmaz
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004220:Disulfides / Q000097:blood; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006710:Homocysteine / Q000097:blood*; D006728:Hormones / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014421:Turkey; D055815:Young Adult
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D015415:Biomarkers; D004220:Disulfides; D006728:Hormones; D008055:Lipids; D006710:Homocysteine; D009569:Nitric Oxide; D008315:Malondialdehyde