Microsatellite primers in luohanguo (Siraitia grosvenorii, Cucurbitaceae), an economically important plant species.
OBJECTIVE
Microsatellite markers were developed in an economically important plant, Siraitia grosvenorii, to evaluate its genetic diversity.


RESULTS
Using the combined biotin capture method, 15 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from two to 12 across 98 individuals from cultivars and a wild population. The observed and expected heterozygosities ranged from 0.000 to 0.905 and from 0.000 to 0.845, respectively.


CONCLUSIONS
These markers will facilitate the breeding and further study of the genetic diversity of S. grosvenorii.
Ministry of Education Key Laboratory for Ecology of Rare and Endangered Species, and Environmental Protection, School of Life Sciences, Guangxi Normal University, Guilin 541004, Guangxi, China.
Yaofang
Y
Wang
Qiwei
Q
Zhang
Yanfang
Y
Lin
Boling
B
Li
Shaoqing
S
Tang
D000483:Alleles; D018556:Crops, Agricultural / Q000235:genetics; D019662:Cucurbitaceae / Q000235:genetics*; D017931:DNA Primers; D018744:DNA, Plant; D005828:Genetics, Population; D006579:Heterozygote; D008516:Medicine, Chinese Traditional; D018895:Microsatellite Repeats; D010946:Plants, Medicinal / Q000235:genetics; D011110:Polymorphism, Genetic
D017931:DNA Primers; D018744:DNA, Plant