Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark.
This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with IC₅₀ values 12.00 ± 0.45, 49.40 ± 1.58 µg and 125.42 ± 2.82 µg mL⁻¹, respectively. In a zymogram assay, the extract showed differential inhibition towards hyaluronidase isoform preferentially with low-molecular weight isoforms. The V. russelli snake venom-induced hemorrhage was significantly reduced at 1:05 ratio of venom-to-extract in mouse. The high antioxidant activity and total phenolic content in the ethanolic extract strongly correlated with the hyaluronidase inhibition. The above results justify the traditional use of the stem bark of B. monosperma as a contraceptive and a strong antidote to snake venom.
Department of Studies in Biochemistry, University of Mysore, Mysore 570006, India.
Shaista
S
Tarannum
Riyaz
R
Mohamed
Bannikuppe S
BS
Vishwanath
D000818:Animals; D031290:Butea / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D008297:Male; D051379:Mice; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D017840:Daboia; D012756:Sheep; D012910:Snake Venoms / Q000201:enzymology*; D013737:Testis / Q000201:enzymology*
D010936:Plant Extracts; D012910:Snake Venoms; D006821:Hyaluronoglucosaminidase