Characterization of cell lysis events on a microfluidic device for high-throughput single cell analysis.
A microfluidic device capable of rapidly analyzing cells in a high-throughput manner using electrical cell lysis is further characterized. In the experiments performed, cell lysis events were studied using an electron multiplying charge coupled device camera with high frame rate (>100  fps) data collection. It was found that, with this microfluidic design, the path that a cell follows through the electric field affects the amount of lysate injected into the analysis channel. Elimination of variable flow paths through the electric field was achieved by coating the analysis channel with a polyamine compound to reverse the electroosmotic flow (EOF). EOF reversal forced the cells to take the same path through the electric field. The improved control of the cell trajectory will reduce device-imposed bias on the analysis and maximizes the amount of lysate injected into the analysis channel for each cell, resulting in improved analyte detection capabilities.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27719, USA.
Amy D
AD
Hargis
Jean Pierre
JP
Alarie
John Michael
JM
Ramsey
D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000295:instrumentation*; D053841:Electroosmosis; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods