The proteome of normal human chorionic villus sampling cells.
Chorionic villi samples are widely used for prenatal diagnosis of various fetal disorders. Although, our knowledge regarding the molecular level of these disorders is extensive, little is known about the implicated proteins. In the present study, two dimensional electrophoresis (2-DE) followed by mass spectrometry (MS) was applied to reveal the proteomic profile of the CV cells. This proteomic technique was previously used successfully in the cases of amniotic fluid, follicular fluid and maternal blood, but has not yet been applied to CV. Therefore, 2-DE was combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS) to characterise the proteome of normal CV cultured cells. Two hundred eighty two-individual gene products were identified including cytoplasmic and nuclear proteins. Although the majority of the proteins were enzymes, structural, signalling and carrier molecules were also isolated. 2D protein map elucidates 282 protein molecules expressed in the CV cells that can be used as a reference for future comparison to various pathological conditions.
Proteomics Research Unit, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Soranou Ephessius 4, 11527 Athens, Greece.
Alexandra G
AG
Xanthopoulou
Athanassios K
AK
Anagnostopoulos
Aggeliki
A
Thanasopoulou
Ema
E
Anastasiadou
Stavros
S
Sifakis
Athanassia
A
Siafaka-Kapadai
George Th
GT
Tsangaris
D015193:Chorionic Villi Sampling; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome