Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion.
Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil. foliveirasilva@gmail.com
Flávio
F
de Oliveira Silva
Priscila
P
das Neves Santos
Cristiane Moutinho Lagos
CM
de Melo
Edson Holanda
EH
Teixeira
Benildo
B
de Sousa Cavada
Francisco Vassiliepe Sousa
FV
Arruda
João Batista
JB
Cajazeiras
Alysson Chaves
AC
Almeida
Valéria Alves Rêgo
VA
Pereira
Ana Lúcia Figueiredo
AL
Porto
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D037121:Plant Lectins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D037121:Plant Lectins; C039268:lectin, Canavalia; D003208:Concanavalin A; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma