Immunolocalization of S100-like protein in the brain of an emerging model organism: Nothobranchius furzeri.
The S100 protein in nervous tissue appears to play important roles in regulating neuronal differentiation, glial proliferation, plasticity, development, axonal growth, and in neurogenetic processes. In fish, the adult neurogenic activity is much higher than in mammals. In this study, the localization of S100 protein was investigated in the brain of annual teleost fish, Nothobranchius furzeri, which is an emerging model organism for aging research. By immunohistochemical techniques, S100 immunoreactivity (IR) was detected in glial cells, small neurons, and fibers throughout all regions of central nervous system (CNS) with different pattern of distribution. In the telencephalon, S100 IR was seen in the olfactory bulbs and in different areas of the telencephalic hemispheres. In the diencephalon, S100 positivity was observed in the habenular nuclei of the epithalamus, in the cortical thalamic nucleus, in the dorsal, ventral and caudal portions, the latter with the posterior recessus nucleus, and in the diffuse inferior lobe of the hypothalamus, along the diencephalic ventricle and in the dorsal optic tract. In the mesencephalon, S100 IR was observed in the longitudinal tori, in the optic tectum, and along the mesencephalic ventricle. In the rhombencephalon, S100 IR was shown in valvula and body of the cerebellum, and in some nuclei of the medulla oblongata. The results suggest that S100 may play a key role in the maintenance of the CNS and in neurogenesis processes in the adulthood.
Department of Biological Structures, Functions and Technology, University of Naples Federico II, 80137 Napoli, Italy.
Livia
L
D'Angelo
Paolo
P
De Girolamo
Alessandro
A
Cellerino
Eva Terzibasi
ET
Tozzini
Ettore
E
Varricchio
Luciana
L
Castaldo
Carla
C
Lucini
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D003532:Cyprinodontiformes / Q000378:metabolism*; D007150:Immunohistochemistry; D023421:Models, Animal; D009457:Neuroglia / Q000737:chemistry / Q000378:metabolism; D009900:Optic Nerve / Q000737:chemistry / Q000378:metabolism; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism*
D009418:S100 Proteins