[Preservation of urine samples for UF 1000i (bioMérieux©) analysis].
With the general tendency that's to say the grouping of laboratories as technical divisions and the accreditation's obligation, the handling of urinary sample preservation's conditions is a major concern. Urinary sample preservation, with or without boric acid, for UF 1000i urinalysis was the purpose of this study. No significant modification was found in red blood cells and white blood cells concentration on a 48  h period with or without boric acid. Preservation's quality of the red blood cells is lower, but the presence of preservative is ineffectual. Thus, for those elements, even if a fast analyse is obviously preferred, these parameters seem to be stable enough to allow a 24  to 48  h delayed urinalysis. Results are totally different for bacteria, which grow if no preservative is added, in a period of time based on the size of the initial bacteria's inoculum. Efficiency of the boric acid as a preservative is shown by the curves, which put into perspective the concentration of acid boric. Finally, boric acid doesn't interfere with the erythrocyte, leucocyte or bacteria's count on UF 1000i and his use is totally suitable for the preservation of urine samples for UF 1000i analysis.
CHR Metz-Thionville, Hôpital Bel-Air, Laboratoire de Microbiologie, Thionville, France. chris.fabbro@yahoo.fr
Christelle
C
Fabbro
Jacques
J
Darolles
Jean-Philippe
JP
Rault
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D058491:Bacterial Load; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods*; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D004906:Erythrocyte Count; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013048:Specimen Handling / Q000379:methods* / Q000592:standards; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D055815:Young Adult