Sensing carbohydrate-protein interactions at picomolar concentrations using cantilever arrays.
Carbohydrates are important mediators of many biological processes that underlie cellular communication and disease mechanisms. Therapeutic agents include carbohydrate-based vaccines and the potent anti-viral protein Cyanovirin-N (CV-N). CV-N acts by specifically binding the carbohydrate structures decorating the cell surface of deadly viruses including human immunodeficiency virus (HI-V) or Ebola. In search for new carbohydrate-binding proteins and the development of sensors that exploit carbohydrate-protein interactions the label-free cantilever array technique can provides a fast, parallel and low-cost approach.
Walther-Meissner-Institute, Ludwig-Maximilians University München, Garching, Germany. kathrin.gruber@wmi.badw.de
Kathrin
K
Gruber
Bianca A
BA
Hermann
Peter H
PH
Seeberger
D001426:Bacterial Proteins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis*; D004926:Escherichia coli / Q000737:chemistry; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry
D001426:Bacterial Proteins; D002241:Carbohydrates; D002352:Carrier Proteins; D011134:Polysaccharides; C107733:cyanovirin N