Expression of MK-1 and RegIV and its clinicopathological significances in the benign and malignant lesions of gallbladder.
BACKGROUND
To study the expression of MK-1 and RegIV and to detect their pathological significances in benign and malignant lesions of gallbladder.


METHODS
The expression of MK-1 and RegIV was detected by immunohistochemical method in paraffin-embedded sections of surgical resected specimens from gallbladder adenocarcinoma (n = 108), peritumoral tissues (n = 46), adenomatous polyp (n = 15), and chronic cholecystitis (n = 35).


RESULTS
The positive rate of MK-1 or RegIV expression was significantly higher in gallbladder adenocarcinoma than that in peritumoral tissues (χ(2)(MK-1) = 18.76, P < 0.01; χ(2)(RegIV) = 9.92, P < 0.01), denomatous polyp (χ(2)(MK-1) = 9.49, P < 0.01; χ(2)(RegIV) = 8.59, P < 0.01) and chronic cholecystitis (χ(2)MK-1 = 24.11, P < 0.01; χ(2)(RegIV) = 19.24, P < 0.01). The positive cases of MK-1 and/or RegIV in the benign lesions showed moderately- or severe-atypical hyperplasia of gallbladder epitheli. The positive rates of MK-1 were significantly higher in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). On the contrary, the positive rates of RegIV were significantly lower in the cases of well-differentiated adenocarcinoma, no-metastasis of lymph node, and no-invasiveness of regional tissues than those in the ones of differentiated adenocarcinoma, metastasis of lymph node, and invasiveness of regional tissues in gallbladder adenocarcinoma (P < 0.05 or P < 0.01). Univariate Kaplan-Meier analysis showed that decreased expression of MK-1 (P = 0.09) or increased expression of RegIV (P = 0.003) was associated with decreased overall survival. Multivariate Cox regression analysis showed that decreased expression of MK-1 (P = 0.033) and increased expression of RegIV (P = 0.008) was an independent prognostic predictor in gallbladder adenocarcinoma.


CONCLUSIONS
The expression of MK-1 and/or RegIV might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma.
Research laboratory of Hepatobiliary Diseases, Second Xiangya Hospital of Central South University, Changsha 410011, China. yangleping@ymail.com
Leping
L
Yang
Sigen
S
Lan
Jieqiong
J
Liu
Zhulin
Z
Yang
D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018256:Adenomatous Polyps / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D002764:Cholecystitis / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D002908:Chronic Disease; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D037181:Lectins, C-Type / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D000073718:Pancreatitis-Associated Proteins; D011379:Prognosis; D016016:Proportional Hazards Models
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C428528:REG4 protein, human