Quantitation of glucosamine sulfate in plasma by HPLC-MS/MS after administration of powder for oral solution formulation.
A rapid method for the quantification of glucosamine in human plasma using high-performance liquid chromatography coupled to tandem mass spectrometry was developed and validated. The sample preparation includes a simple deproteinization step, using D-[1-¹³C] glucosamine hydrochloride as an internal standard. Chromatographic separation was performed on an ACE Ciano column using isocratic elution with acetonitrile and aqueous 2 mM ammonium acetate containing 0.025% formic acid (80:20). Selected reaction monitoring was performed using the transitions m/z 180.1 → m/z 72.1 and m/z 181.0 → m/z 74.6 to quantify glucosamine and internal standard, respectively. The method was validated and proved to be linear, accurate and precise over the range 50-5000 ng/mL of glucosamine. Recovery rates higher than 90% were obtained for both glucosamine and internal standard. No matrix effect was detected in the samples. The validated method was successfully applied to a pharmacokinetic study after oral administration of a powder for oral solution formulation containing glucosamine sulfate.
Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, 74175-100 Goiânia, GO, Brazil. isaccesar@bol.com.br
Isabela Costa
IC
César
Ricardo Martins Duarte
RM
Byrro
Fabiana Fernandes
FF
de Santana E Silva Cardoso
Iram Moreira
IM
Mundim
Leonardo
L
de Souza Teixeira
Kênnia Rocha
KR
Rezende
Enikson Pontes
EP
da Silva
Sandro Antônio
SA
Gomes
Vilmar Alves
VA
de Sousa
Ricardo Rodrigues
RR
Bonfim
Gerson Antônio
GA
Pianetti
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005944:Glucosamine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D008297:Male; D011208:Powders / Q000008:administration & dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D011208:Powders; D005944:Glucosamine