Recovery of macular pigment spectrum in vivo using hyperspectral image analysis.
We investigated the feasibility of a novel method for hyperspectral mapping of macular pigment (MP) in vivo. Six healthy subjects were recruited for noninvasive imaging using a snapshot hyperspectral system. The three-dimensional full spatial-spectral data cube was analyzed using non-negative matrix factorization (NMF), wherein the data was decomposed to give spectral signatures and spatial distribution, in search for the MP absorbance spectrum. The NMF was initialized with the in vitro MP spectrum and rank 4 spectral signature decomposition was used to recover the MP spectrum and optical density in vivo. The recovered MP spectra showed two peaks in the blue spectrum, characteristic of MP, giving a detailed in vivo demonstration of these absorbance peaks. The peak MP optical densities ranged from 0.08 to 0.22 (mean 0.15+∕-0.05) and became spatially negligible at diameters 1100 to 1760 μm (4 to 6 deg) in the normal subjects. This objective method was able to exploit prior knowledge (the in vitro MP spectrum) in order to extract an accurate in vivo spectral analysis and full MP spatial profile, while separating the MP spectra from other ocular absorbers. Snapshot hyperspectral imaging in combination with advanced mathematical analysis provides a simple cost-effective approach for MP mapping in vivo.
University of Southern California, Doheny Eye Institute, Keck School of Medicine, Department of Ophthalmology, 1450 San Pablo Ave, Suite 3614, Los Angeles, California 90033, USA.
Amani A
AA
Fawzi
Noah
N
Lee
Jennifer H
JH
Acton
Andrew F
AF
Laine
R Theodore
RT
Smith
D000328:Adult; D005127:Eye Color; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000706:statistics & numerical data; D021621:Imaging, Three-Dimensional / Q000379:methods / Q000706:statistics & numerical data; D008266:Macula Lutea / Q000033:anatomy & histology* / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008875:Middle Aged; D055620:Optical Phenomena; D012168:Retinal Pigments / Q000737:chemistry*; D055815:Young Adult
D012168:Retinal Pigments