In vivo two-photon microscopy to 1.6-mm depth in mouse cortex.
Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.
Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853, USA. dk287@cornell.edu
Demirhan
D
Kobat
Nicholas G
NG
Horton
Chris
C
Xu
D000818:Animals; D002540:Cerebral Cortex / Q000033:anatomy & histology* / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D005456:Fluorescent Dyes / Q000008:administration & dosage; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055620:Optical Phenomena
D005456:Fluorescent Dyes