Polyarginine peptide IND-1 enhances recombinant human bone morphogenetic protein-2 yield in mammalian cells.
To improve recombinant human bone morphogenetic protein-2 (rhBMP-2) yield, cell lines stably expressing hBMP2 were cultured in the presence of polyarginine peptide IND-1 and showed up to 6-fold increase in the yield of mature BMP-2. Repeated addition of IND-1 to cell cultures consistently improved BMP-2 yields over 53 days without affecting cell growth and viability. Investigation of its mechanism of action showed that IND-1 inhibited pro-protein convertase (PC) activity when incubated with cell lysates. However, when intact cells were cultured with IND-1, no change in cellular PC activity was observed. Furthermore, knockdown of furin (a prototypical member of the PCs) in cells did not affect their BMP-2 yields, suggesting furin/PC inhibition is unlikely the mechanism by which IND-1 enhances BMP-2 yields. IND-1 as a medium additive thus enhances BMP-2 production in mammalian cell expression systems.
Oral & Maxillofacial Surgery, Faculty of Dentistry, University of Toronto, 124 Edward Street, Room 412, Toronto, Toronto, ON, M5G 1G6, Canada.
Aileen J
AJ
Zhou
Cameron M L
CM
Clokie
Sean A F
SA
Peel
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D055396:Bone Morphogenetic Protein 2; D003470:Culture Media; D010455:Peptides; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C015462:polyarginine