Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
The aim of this study was to discriminate 30 Vibrio strains isolated from two wastewater treatment plants from Agadir, Morocco by two molecular typing methods, pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Out of the 30 strains of Vibrio examined in this study, 5 isolates could not be typed by PFGE and consistently appeared as a smear on the gel. In general, high genetic biodiversity among the Vibrio strains was found regardless to the isolation source. The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS.
UPRES EA-4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de Médecine, Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, France. eddabra@gmail.com
Rkia
R
Eddabra
Gilles
G
Prévost
Jean-Michel
JM
Scheftel
D001431:Bacteriological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009018:Morocco; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D014733:Vibrio / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014871:Water Microbiology