Production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase PduP mediated biosynthetic pathway in Klebsiella pneumoniae.
The pduP gene encodes a propionaldehyde dehydrogenase (PduP) was investigated for the role in 3-hydroxypropionic acid (3-HP) glycerol metabolism in Klebsiella pneumoniae. The enzyme assay showed that cell extracts from a pduP mutant strain lacked measurable dehydrogenase activity. Additionally, the mutant strain accumulated the cytotoxic intermediate metabolite 3-hydroxypropionaldehyde (3-HPA), causing both cell death and a lower final 3-HP titer. Ectopic expression of pduP restored normal cell growth to mutant. The enzymatic property of recombinant protein from Escherichia coli was examined, exhibiting a broad substrate specificity, being active on 3-HPA. The present work is thus the first to demonstrate the role of PduP in glycerol metabolism and biosynthesis of 3-HP.
Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea.
Lian Hua
LH
Luo
Chul Ho
CH
Kim
Sun-Yeon
SY
Heo
Baek-Rock
BR
Oh
Won-Kyung
WK
Hong
Seonghun
S
Kim
Dae-Hyuk
DH
Kim
Jeong-Woo
JW
Seo
D000447:Aldehydes / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D017353:Gene Deletion; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D020123:Sirolimus / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects
D000447:Aldehydes; D001426:Bacterial Proteins; D019344:Lactic Acid; C005556:propionaldehyde; C031601:hydracrylic acid; D010088:Oxidoreductases; D020123:Sirolimus