Tentative identification of new metabolites of epimedin C by liquid chromatography-mass spectrometry.
Epimedin C is one of the major bioactive constituents of Herba Epimedii. The aim of this study is to characterize and elucidate the structure of metabolites in the rat after administration of epimedin C. Metabolite identification was performed using a predictive multiple reaction monitoring-information dependent acquisition-enhanced product ion (pMRM-IDA-EPI) scan in positive ion mode on a hybrid triple quadrupole-linear ion trap mass spectrometer. A total of 18 metabolites were characterized by the changes in their protonated molecular masses, their MS/MS spectrum and their retention times compared with those of the parent drug. The results reveal possible metabolite profiles of epimedin C in rats; the metabolic pathways including hydrolysis, hydroxylation, dehydrogenation, demethylation and conjugation with glucuronic acid and different sugars were observed. This study provides a practical approach for rapidly identifying complicated metabolites, a methodology that could be widely applied for the structural characterization of metabolites of other compounds.
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, PR China.
Minyan
M
Liu
Shaohua
S
Zhao
Zongquan
Z
Wang
Hongtao
H
Wang
Xiaowei
X
Shi
Ziming
Z
Lü
Honghui
H
Xu
Hairong
H
Wang
Yingfeng
Y
Du
Lantong
L
Zhang
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010936:Plant Extracts; C046314:epimedii flavone; C106054:epimedin C