Determination of deoxynivalenol in cereals by immunoaffinity clean-up and ultra-high performance liquid chromatography tandem mass spectrometry.
An immunoaffinity column (IAC) was prepared with a new deoxynivalenol (DON) monoclonal antibody and used as a clean-up tool before ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis of DON in cereals. The developed IAC clean-up method showed high recoveries for DON. They ranged from 61% to 103% in wheat, rice, and millet with intra-day and inter-day variations below 19% and 17%, respectively. The column capacity was 2.86μg DON per mL of gel, and it maintained above 0.68μg/mL of gel after 10 cycles of usage at 2 days intervals. The limit of detection (LOD) and limit of quantification (LOQ) were 0.3 and 0.8μg/kg, respectively. Twenty-one out of 40 analyzed commercial cereal samples were positive at DON concentrations from 7 to 534μg/kg.
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Yanshen
Y
Li
Zhanhui
Z
Wang
Sarah
S
De Saeger
Weimin
W
Shi
Cun
C
Li
Suxia
S
Zhang
Xingyuan
X
Cao
Jianzhong
J
Shen
D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D018556:Crops, Agricultural / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D013997:Time Factors; D014255:Trichothecenes / Q000032:analysis* / Q000737:chemistry
D014255:Trichothecenes; C007262:deoxynivalenol