RNA interference for performance enhancement and detection in doping control.
RNA interference represents a comparably new route of regulating and manipulating specific gene expression. Promising results were obtained in experimental therapies aim at the treatment of different kinds of diseases including cancer, diabetes mellitus or Dychenne muscular dystrophy. While studies on down-regulation efficiency are often performed by analyzing the regulated protein, the direct detection of small, interfering RNA molecules and antisense oligonucleotides is of great interest for the investigation of the metabolism and degradation and also for the detection of a putative misuse of these molecules in sports. Myostatin down-regulation was shown to result in increased performance and muscle growth and the regulation of several other proteins could be relevant for performance enhancement. This mini-review summarizes current approaches for the mass spectrometric analysis of siRNA and antisense oligonucleotides from biological matrices and the available data on biodistribution, metabolism, and half-life of relevant substances are discussed.
Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany.
Maxie
M
Kohler
Wilhelm
W
Schänzer
Mario
M
Thevis
D000818:Animals; D004300:Doping in Sports / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D016376:Oligonucleotides, Antisense / Q000032:analysis* / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000032:analysis* / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology*
D016376:Oligonucleotides, Antisense; D034741:RNA, Small Interfering