Color capable sub-pixel resolving optofluidic microscope and its application to blood cell imaging for malaria diagnosis.
Miniaturization of imaging systems can significantly benefit clinical diagnosis in challenging environments, where access to physicians and good equipment can be limited. Sub-pixel resolving optofluidic microscope (SROFM) offers high-resolution imaging in the form of an on-chip device, with the combination of microfluidics and inexpensive CMOS image sensors. In this work, we report on the implementation of color SROFM prototypes with a demonstrated optical resolution of 0.66 µm at their highest acuity. We applied the prototypes to perform color imaging of red blood cells (RBCs) infected with Plasmodium falciparum, a particularly harmful type of malaria parasites and one of the major causes of death in the developing world.
Department of Electrical Engineering, California Institute of Technology, Pasadena, California, United States of America. salee30@caltech.edu
Seung Ah
SA
Lee
Ricardo
R
Leitao
Guoan
G
Zheng
Samuel
S
Yang
Ana
A
Rodriguez
Changhuei
C
Yang
D003116:Color; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D008288:Malaria / Q000097:blood* / Q000175:diagnosis*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D010963:Plasmodium falciparum / Q000502:physiology