Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated β-glucuronidase reporter gene expression system.
The transgenic tobacco plant XD4V-26 carrying the recombinant mouse aryl hydrocarbon receptor XD4V-mediated β-glucuronidase (GUS) reporter gene expression system was used for assay of dioxins and dioxin-like compounds consisting of polychlorinated dibenzeno-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls (Co-PCBs) in actually contaminated soils. The transgenic tobacco plant XD4V-26 showed a significant dose-dependent induced GUS activity when cultured on MS medium containing PCB126 [toxic equivalency factor (TEF) = 0.1]. In contrast, PCB169 and PCB180, which have 0.03 of TEF and unassigned TEF values, respectively, did not significantly induce GUS activity under the same conditions as with PCB126. When the tobacco plants were cultivated for up to 5 weeks on actually contaminated soils with dioxins and dioxin-like compounds collected from the periphery of an incinerator used for disposal of residential and industrial wastes, GUS activity in the leaves was dose-dependently increased. The plants clearly detected 360 pg-TEQ g(-1) of dioxins and dioxin-like compounds in this assay. There was a positive correlation between GUS activity and TEQ value of dioxins and dioxin-like compounds in the plants. This assay does not require any extraction and purification processes for the actually contaminated soil samples.
Research Center for Environmental Genomics, Kobe University, Hyogo, Japan.
Hideyuki
H
Inui
Keiko
K
Gion
Yasushi
Y
Utani
Taketo
T
Wakai
Susumu
S
Kodama
Heesoo
H
Eun
Yun-Seok
YS
Kim
Hideo
H
Ohkawa
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D004147:Dioxins / Q000032:analysis / Q000633:toxicity*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D017930:Genes, Reporter / Q000187:drug effects*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects
D051792:Basic Helix-Loop-Helix Proteins; D004147:Dioxins; D005966:Glucuronidase; D011078:Polychlorinated Biphenyls; D018336:Receptors, Aryl Hydrocarbon; D012989:Soil Pollutants; C484722:Ahr protein, mouse