DNAzyme-based fluorescent microarray for highly selective and sensitive detection of lead(II).
A facile microarray-based fluorescent sensor for the detection of lead (II) was developed based on the catalytic cleavages of the substrates by a DNAzyme upon its binding to Pb(2+). The release of the fluorophore labelled substrates resulted in the decrease of fluorescence intensity. The sensor had a quantifiable detection range from 1 nM to 1 μM and a selectivity of >20 fold for Pb(2+) over other metal ions.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Changning Rd. 865, Shanghai, China 200050.
Meiying
M
Liu
Xinhui
X
Lou
Juan
J
Du
Ming
M
Guan
Jing
J
Wang
Xiaofan
X
Ding
Jianlong
J
Zhao
D015374:Biosensing Techniques / Q000379:methods*; D020134:Catalytic Domain; D002413:Cations, Divalent; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes; D007854:Lead / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D002413:Cations, Divalent; D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D007854:Lead