Molecular phylogeny of the Myzorhynchella Section of Anopheles (Nyssorhynchus) (Diptera: Culicidae): genetic support for recently described and resurrected species.
Phylogenetic relationships among species of the Myzorhynchella Section of Anopheles (Nyssorhynchus) were investigated using the nuclear ribosomal DNA second internal transcribed spacer (ITS2), the nuclear whitegene and mitochondrial cytochrome oxidase subunit I (COI) regions. The recently described Anopheles pristinus and resurrected Anopheles guarani were also included in the study. Bayesian phylogenetic analyses found Anopheles parvus to be the most distantly related species within the Section, a finding that is consistent with morphology. An. pristinus and An. guarani were clearly resolved from Anopheles antunesi and Anopheles lutzii, respectively. An. lutzii collected in the same mountain range as the type locality were found within a strongly supported clade, whereas individuals from the southern state of Rio Grande do Sul, tentatively identified as An. lutzii based on adult female external morphology, were distinct from An. lutzii, An. antunesi and from each other, and may therefore represent two new sympatric species. A more detailed examination of An. lutzii sensu latoalong its known geographic range is recommended to resolve these anomalous relationships.
Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil.
Brian Patrick
BP
Bourke
Sandra Sayuri
SS
Nagaki
Eduardo Sterlino
ES
Bergo
Jáder da Cruz
Jda C
Cardoso
Maria Anice Mureb
MA
Sallum
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001499:Bayes Theorem; D002467:Cell Nucleus / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D017344:Genes, Insect / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology; D010802:Phylogeny; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV