Generation of HIV latency in humanized BLT mice.
Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor latently infected resting human CD4+ T cells that can be induced ex vivo to produce HIV. We observed that the levels of infected resting human CD4+ T cells present in BLT mice are within the range of those observed circulating in patients undergoing suppressive ART. These results demonstrate the potential of humanized BLT mice as an attractive model for testing the in vivo efficacy of novel HIV eradication strategies.
Division of Infectious Diseases, Department of Internal Medicine, Center for AIDS Research, University of North Carolina, Chapel Hill, North Carolina, USA.
Paul W
PW
Denton
Rikke
R
Olesen
Shailesh K
SK
Choudhary
Nancy M
NM
Archin
Angela
A
Wahl
Michael D
MD
Swanson
Morgan
M
Chateau
Tomonori
T
Nochi
John F
JF
Krisko
Rae Ann
RA
Spagnuolo
David M
DM
Margolis
J Victor
JV
Garcia
D000818:Animals; D019380:Anti-HIV Agents; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D004195:Disease Models, Animal; D006678:HIV / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000821:virology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D017735:Virus Latency / Q000187:drug effects
D019380:Anti-HIV Agents