Interfacial electrochemical electron transfer in biology - towards the level of the single molecule.
Physical electrochemistry has undergone a remarkable evolution over the last few decades, integrating advanced techniques and theory from solid state and surface physics. Single-crystal electrode surfaces have been a core notion, opening for scanning tunnelling microscopy directly in aqueous electrolyte (in situ STM). Interfacial electrochemistry of metalloproteins is presently going through a similar transition. Electrochemical surfaces with thiol-based promoter molecular monolayers (SAMs) as biomolecular electrochemical environments and the biomolecules themselves have been mapped with unprecedented resolution, opening a new area of single-molecule bioelectrochemistry. We consider first in situ STM of small redox molecules, followed by in situ STM of thiol-based SAMs as molecular views of bioelectrochemical environments. We then address electron transfer metalloproteins, and multi-centre metalloenzymes including applied single-biomolecular perspectives based on metalloprotein/metallic nanoparticle hybrids.
Department of Chemistry, DTU Chemistry, Building 207, Kemitorvet, Technical University of Denmark, DK 2800 Kongens Lyngby, Denmark.
Jingdong
J
Zhang
Qijin
Q
Chi
Allan G
AG
Hansen
Palle S
PS
Jensen
Princia
P
Salvatore
Jens
J
Ulstrup
D004563:Electrochemistry / Q000379:methods* / Q000639:trends; D004579:Electron Transport; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods; D016252:Microscopy, Scanning Tunneling / Q000379:methods; D008958:Models, Molecular; D011487:Protein Conformation; D015203:Reproducibility of Results
D008667:Metalloproteins; D006046:Gold