Luciferase and luciferin co-immobilized mesoporous silica nanoparticle materials for intracellular biocatalysis.
We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP- and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.
Department of Chemistry, U.S. Department of Energy, and Ames Laboratory, Iowa State University, Ames, Iowa 50011-3111, USA.
Xiaoxing
X
Sun
Yannan
Y
Zhao
Victor S-Y
VS
Lin
Igor I
II
Slowing
Brian G
BG
Trewyn
D052160:Benzothiazoles / Q000378:metabolism; D055162:Biocatalysis; D016503:Drug Delivery Systems; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
D052160:Benzothiazoles; C532924:D-luciferin; D006046:Gold; D012822:Silicon Dioxide; D008156:Luciferases