Enhanced Y1H assays for Arabidopsis.
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
Department of Plant Biology and Genome Center, University of California Davis, Davis, California, USA.
Allison
A
Gaudinier
Lifang
L
Zhang
John S
JS
Reece-Hoyes
Mallorie
M
Taylor-Teeples
Li
L
Pu
Zhijie
Z
Liu
Ghislain
G
Breton
Jose L
JL
Pruneda-Paz
Dahae
D
Kim
Steve A
SA
Kay
Albertha J M
AJ
Walhout
Doreen
D
Ware
Siobhan M
SM
Brady
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D014157:Transcription Factors