Toxoplasma gondii: identification and immune response against a group of proteins involved in cellular invasion.
Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites.
Department of Parasitology and Medical Mycology, Faculté de Pharmacie, Université de Lyon, Université Claude Bernard-Lyon I, ISPB, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France. samira.azzouz-maache@univ-lyon1.fr
Samira
S
Azzouz
Mimoun
M
Maache
Antonio
A
Osuna
Philippe
P
Lawton
Anne Françoise
AF
Pétavy
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D016516:CD4-CD8 Ratio; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification*
D000913:Antibodies, Protozoan; D016207:Cytokines; D007074:Immunoglobulin G; D015800:Protozoan Proteins