Detection of schistosomiasis antibodies in urine patients as a promising diagnostic maker.
OBJECTIVE
To determine secreted antischistosoma antibodies in urine and to discern the epidemiological situation of schistosomiasis in the agricultural field labourers'camps city in the Gezira State-central Sudan.


METHODS
Total of 66 urine and 66 serum paired samples were collected from those who confirmed parasitologically positive and negative with schistosomiasis from the two camps. Samples were tested using ELISA technique to measure and compare the immunoglobulin G (IgG) levels in serum and urine samples of schistosomiasis patients.


RESULTS
The overall prevalence of S. mansoni and S. haematobium was 53.8% and 15.4%, while the intensity were (2.04 GMEC) and (0.9 GMEC) respectively. The relative percentage of positive IgG individulas in urine was 92.40% where as 96.97% in serum. Statistically no significant difference between the IgG levels in serum and urine samples was observed.


CONCLUSIONS
This study shows that the detection of secreted IgG antibodies in urine can substitute serum for diagnosis of schistosomiasis.
Department of Vector and Biomedical Studies, Tropical Medicine Research Institute, National Centre for Research, Khartoum, Sudan. selmamedani@hotmail.com
Salma Medani
SM
Elhag
Eshtiyag Abdalla
EA
Abdelkareem
Ashraf Siddig
AS
Yousif
Ehab Ahmed
EA
Frah
Ahmed Babiker
AB
Mohamed
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000382:Agricultural Workers' Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000652:urine*; D015415:Biomarkers / Q000652:urine; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005240:Feasibility Studies; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010270:Parasite Egg Count; D015995:Prevalence; D012548:Schistosoma haematobium / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D013397:Sudan / Q000453:epidemiology; D055815:Young Adult
D000909:Antibodies, Helminth; D015415:Biomarkers; D007074:Immunoglobulin G