The absorption characterization effects and mechanism of Radix Angelicae dahuricae extracts on baicalin in Radix Scutellariae using in vivo and in vitro absorption models.
BACKGROUND
Angelicae Dahurica(Hoffm.)Benth.&Hook.f.ex Franch.&Sav combined with Scutellaria baicalensis Georgi. has been widely used as herb-pairs in traditional Chinese medicine (TCM) to treat migraine headache and cataract, but the underlying compatibility mechanism of the two herbs remains unknown.


OBJECTIVE
In the present work, we investigated the additive or synergistic effects of absorption behavior of Radix Angelicae dahuricae extracts on baicalin, and the absorption-enhancing mechanism of Radix Angelicae dahuricae extracts on baicalin.


METHODS
Total coumarins (Cou) and volatile oil (VO), as the two main components of Radix Angelicae dahuricae, were extracted by supercritical fluid extraction (SFE) further treated with liquid-liquid separation method. The absorption behavior was investigated by applying the everted gut sac technique and in situ single-pass intestinal perfusion method.


CONCLUSIONS
The results showed that both the Cou and the VO could improve the intestinal absorption of baicalin in vivo, and had synergistic action the enhanced absorption of baicalin. Since verapamil did not affect the P(app) and K(a) of baicalin significantly, we concluded that the absorption of Baicalin could not be an active transportation in dependent of P-glycoprotein-Mediated efflux systems. Based on intestinal absorption of drug studying was one of the efficacious methods to clarify the compatibility of principles of herb-pairs. The everted gut sac technique and in situ single-pass intestinal perfusion technique model were the effective methods to study the absorption of drug, the application of the animal model to investigating the absorption of herb-drug interactions or other relevant research purposes is envisioned.
Key Laboratory of Modern Preparation of TCM (Jiangxi University of Traditional Chinese Medicine), Ministry of Education, Nanchang 330004, China.
Xin-Li
XL
Liang
Zheng-Gen
ZG
Liao
Jing-Yun
JY
Zhu
Guo-Wei
GW
Zhao
Ming
M
Yang
Rong-Li
RL
Yin
Yun-Chao
YC
Cao
Jing
J
Zhang
Li-Jun
LJ
Zhao
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D029969:Angelica; D000818:Animals; D003374:Coumarins / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D041743:Herb-Drug Interactions; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D009822:Oils, Volatile / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D009822:Oils, Volatile; C038044:baicalin