[Effects of tongguan capsule on the mobilization and differentiation of bone marrow mononuclear cells to the injured carotid artery].
OBJECTIVE
To observe the effects of Tongguan Capsule on the mobilization and differentiation of bone marrow mononuclear cells (BMMNCs) to the injured carotid arteries.


METHODS
The rat model of injured carotid arteries was established. Mononuclear cells were separated from the bone marrow of donor rats, which were labeled by PKH 26 and then injected through the tail vein to the recipient rats with injured carotid arteries. The rats were randomly divided into two groups. Tongguan Capsule suspension was administered by gastrogavage to rats in the experiment group, while equal volume of normal saline was given to rats in the control group. Four weeks later the injured carotid arteries were harvested and frozen sections were made to observe the differences of intima area/media area ratio (Ai/Am). The difference of BMMNCs migrating to the injured carotid arteries between the two groups was observed under fluorescence microscope. The difference of the vascular endothelial growth factor (VEGF) and stromal derived factor-1 (SDF-1) levels in serum were detected by ELISA.


RESULTS
The PKH 26 labeled cells appeared in the experiment group, while only little scattered fluorescent light points was presented in the control group. The intima area and the ratio of Ai/Am of the experiment group decreased more obviously than that of the control group (P<0.01). Two and 4 weeks later the VEGF and SDF-1 levels in serum of the experiment group were more obviously enhanced than those of the control group (P<0.01).


CONCLUSIONS
Tongguan Capsule could promote the BMMNCs transplanting towards the intima of injured carotid arteries, and take part in the repair of the intima of injured carotid arteries.
ICU, The Second Affiliated Hospital of Guangdong University of Traditional Chinese Medicine, Guangzhou 510120.
Peng
P
Chen
Jian
J
Zhang
Lei
L
Wang
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017539:Tunica Intima / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; D004365:Drugs, Chinese Herbal; D042461:Vascular Endothelial Growth Factor A; C532895:tongguan; C467498:vascular endothelial growth factor A, rat