An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato.
A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive factor (ERF) superfamily. Analysis of the deduced SlERF5 protein sequence showed that it contained an ERF domain and belonged to the class III group of ERFs proteins. Expression of SlERF5 was induced by abiotic stress, such as high salinity, drought, flooding, wounding and cold temperatures. Over-expression of SlERF5 in transgenic tomato plants resulted in high tolerance to drought and salt stress and increased levels of relative water content compared with wild-type plants. This study indicates that SlERF5 is mainly involved in the responses to abiotic stress in tomato.
College of Bioengineering, Chongqing University, Chongqing, 400044, People's Republic of China. yu.pan82@yahoo.com
Yu
Y
Pan
Graham B
GB
Seymour
Chungui
C
Lu
Zongli
Z
Hu
Xuqing
X
Chen
Guoping
G
Chen
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013696:Temperature; D014867:Water
D018076:DNA, Complementary; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014867:Water