Monitoring of Leuconostoc population during sauerkraut fermentation by quantitative real-time polymerase chain reaction.
A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).
Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Korea.
So-Young
SY
Kim
Ki-Seon
KS
Yoo
Yujin
Y
Kim
Eunyoung
E
Seo
Beom Soo
BS
Kim
Nam Soo
NS
Han
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D007934:Leuconostoc / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014675:Vegetables / Q000378:metabolism / Q000382:microbiology*
D001426:Bacterial Proteins; D005964:Glucosyltransferases; C020025:dextransucrase