Quality assessment of commercial Magnoliae officinalis Cortex by ¹H-NMR-based metabolomics and HPLC methods.
BACKGROUND
The quality control of Magnoliae officinalis Cortex, a commonly used traditional Chinese medicine, is currently based on the assay of the two active compounds, honokiol and magnolol, by TLC or HPLC.


OBJECTIVE
To compare ¹H-NMR-based metabolomics with the HPLC method for controlling the quality of Magnoliae officinalis Cortex. To identify the metabolites contributing to the differences between the samples and to discriminate different medicinal parts and geographic origins of these samples by ¹H-NMR-based metabolomics.


METHODS
¹H-NMR and several multivariate analysis techniques were applied to analyse the extracts of 18 batches of Magnoliae officinalis Cortex commercial samples, and the contents of honokiol and magnolol in these samples were determined by HPLC. The correlation analysis between the data from ¹H-NMR and HPLC was performed with the mixOmics software based on an unsupervised method.


RESULTS
Honokiol and magnolol were the main compounds responsible for the discrimination of samples from different batches, thus proving that the choice of these two compounds as markers for quality assessment by HPLC is relevant. The two sources of Magnoliae officinalis Cortex recorded in the Chinese Pharmacopoeia, Magnolia officinalis and Magnolia officinalis var. biloba, could be differentiated from ¹H-NMR data, but the pattern recognition analysis by PLS-DA was unsuccessful in discriminating samples from various geographical origins.


CONCLUSIONS
The combination of ¹H-NMR that gives a comprehensive profile of the metabolites and HPLC that targets two biomarkers is an efficient means for a better quality control of Magnoliae officinalis Cortex.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. yongjiang@bjmu.edu.cn
Yong
Y
Jiang
Julie
J
Vaysse
Véronique
V
Gilard
Stéphane
S
Balayssac
Sébastien
S
Déjean
Myriam
M
Malet-Martino
Bruno
B
David
Christel
C
Fiorini
Yves
Y
Barbin
D001713:Biphenyl Compounds / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D017753:Ecosystem; D017705:Lignans / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D031566:Magnolia / Q000737:chemistry*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D011786:Quality Control; D012680:Sensitivity and Specificity; D012984:Software; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D017705:Lignans; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols