Suitability of TBA method for the evaluation of the oxidative effect of non-water-soluble and water-soluble rosemary extracts.
The antioxidative effects of rosemary and grape-seed extracts spiked in human plasma were examined using the thiobarbituric acid (TBA) method. The TBA values of plasma spiked with reagents to generate reactive oxygen species, such as singlet oxygen ((1)O(2)), hydroxyl radicals ((·)OH), peroxynitrite (ONOO(-)), and superoxide anions (O(2)(·-)), were measured by a flow injection analysis method with fluorescence (FL) detection. TBA values obtained by the addition of 50 mg/mL of rosemary extracts for (1)O(2), (·)OH, ONOO(-), and O(2)(·-) increased to 964 ± 65%, 1063 ± 61%, 758 ± 78%, and 698 ± 41%, respectively (n = 3, P < 0.01), whereas the values with 1 mg/mL of grape-seed extracts or tocopherol decreased (40.2 - 66.3%). Furthermore, the antioxidative effects of rosemary extract in rat plasma, spiked with reagents to generate (·)OH, were examined by high-performance liquid chromatography with FL detection. No peak, other than TBA-malondialdehyde, could be detected using wavelengths of 532 (λ(ex)) and 553 nm (λ(em)).
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Japan.
Mitsuhiro
M
Wada
Minori
M
Nagano
Hirotsugu
H
Kido
Rie
R
Ikeda
Naotaka
N
Kuroda
Kenichiro
K
Nakashima
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015203:Reproducibility of Results; D027542:Rosmarinus / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012995:Solubility; D013858:Thiobarbiturates / Q000097:blood / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013858:Thiobarbiturates; D014867:Water; D008315:Malondialdehyde; C029684:thiobarbituric acid