Bioaccumulation, depuration and biotransformation of 4,4'-dibromodiphenyl ether in crucian carp (Carassius auratus).
Polybrominated diphenyl ethers (PBDEs) are extensively used as a class of flame retardants and have become ubiquitous environmental pollutants. Significant biotransformation of some PBDEs via reductive debromination has been observed. However, little is known about the fate of lower brominated BDEs in fish. In this study, the tissue distribution, excretion, depuration and biotransformation of 4,4'-dibromodiphenyl ether (BDE 15) were investigated in crucian carp (Carassius auratus) which were exposed to spiked water solution at different concentrations for 50d, followed by a 14-d depuration period. Bioaccumulation parameters were calculated and the results showed that BDE 15 was mainly concentrated in the gill and liver. In particular, five biotransformation products of BDE 15 in carp were identified using GC-MS/MS. Besides two debrominated metabolites, three of the metabolites were mono-OH-BDE 15, diOH-BDE 15 and bromophenol. Our results unequivocally suggested that BDE 15 oxidation did occur via the formation of hydroxylated (OH-) metabolites in crucian carp exposed in vivo. These findings will be useful for determination of the metabolic pathways of PBDEs in freshwater fish, especially about their oxidation metabolism.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, PR China.
Jie
J
Cheng
Liang
L
Mao
Zhigang
Z
Zhao
Mengnan
M
Shen
Shenghu
S
Zhang
Qingguo
Q
Huang
Shixiang
S
Gao
D000818:Animals; D001711:Biotransformation; D005411:Flame Retardants / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical; C558403:dibromodiphenyl ether