[The effect of the compatibility of Radix Aconiti Laterlis and radix glycyrrhizae on pharmacokinatic of aconitine, mesaconitine and hypacmitine in rat plasma].
OBJECTIVE
To established an HPLC-MS assay to determine aconitine, mesaconitine and hypaconitine simultaneously in rat plasma and be used to investigate the pharmacokinetics of the three alkaloids.


METHODS
Three groups of rats were orally administered respectively with three decoctions including decoction of Radix Aconiti Laterlis, blend decoction of Radix Aconiti Laterlis and Radix Glycyrrhizae which decocted separately, decoction of Radix Aconiti Laterlis and Radix Glycyrrhizae which decocted together,the dosage of Radix Aconiti Laterlis was 1.5 g/kg. The contents of aconitine, mesaconitine and hypaconitine in rat plasma were detected using a liquid Chromatography-electrospray ionization/tandem mass spectrometry method. Pharmacokinetic parameters were estimated using DAS 2.0.


RESULTS
The pharmacokinetic parameters of the three compounds obtained showed that Cmax and AUC of aconitine, mesaconitine and hypaconitine were decreased. MRT, t1/2 were prolonged and there was no obviously change in Tmax when Radix Aconiti Lateralis was combined with Radix Glycyrrhizae. The effect of decoction which decocted together was more prominent than which decocted separately.


CONCLUSIONS
There are obvious effects on pharmacokinetic of Aconitine, Mesaconitine and Hypaconitine in Rat Plasma when Radix Aconiti Laterlis is combined with Radix Glycyrrhizae.
Jiangsu Provincial Insititute of Traditional Chinese Medicine, Jiangsu Research Center for Modern TCM Pharmaceutics Process, Nanjing 210028, China. smalleye88@yahoo.cn
Hong
H
Shen
Ling-ying
LY
Zhu
Nan
N
Yao
Jie
J
Wu
D000157:Aconitine / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D031841:Aconitum / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D006035:Glycyrrhiza / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010599:Pharmacokinetics; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
D004365:Drugs, Chinese Herbal; C058309:hypaconitine; C019470:mesaconitine; D000157:Aconitine