Colistin A and colistin B among inhibitory substances of Paenibacillus polymyxa JB05-01-1.
Recently, we isolated and reported the antagonism of Paenibacillus polymyxa JB05-01-1 (P. polymyxa JB05-01-1) against Gram-negative bacteria. Here, we provide more insights and attribute the abovementioned antagonism to the production of colistins A and B, which were purified by Amberlite column exchange, C18 column hydrophobicity, superdex 75 16/60 gel filtration chromatography connected to fast protein liquid chromatography and identified by MALDI TOF/TOF, and manual nanospray analysis. The amount of colistin A and colistin B recovered from 500 ml of culture supernatant was about 0.05 mg. The specific activity and the average recovery of the eluted substances were 5,120 AU/mg and 1.1%, respectively. The minimal inhibitory concentrations of the purified colistins against Escherichia coli O157:H7 and Pseudomonas fluorescens LRC R73 were 0.13 and 0.62 μg/ml, respectively.
Lethbridge Research Center, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Karim
K
Naghmouchi
Riadh
R
Hammami
Ismail
I
Fliss
Ron
R
Teather
John
J
Baah
Djamel
D
Drider
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D003091:Colistin / Q000737:chemistry; D019453:Escherichia coli O157 / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D056507:Paenibacillus / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; C579082:colistin A, Paenibacillus polymyxa JB05-01-1; C579083:colistin B, Paenibacillus polymyxa JB05-01-1; D003091:Colistin