Use of conventional bioanalytical devices to automate DBS extractions in liquid-handling dispensing tips.
BACKGROUND
Conventional liquid-handling devices were employed, along with an improved punching device, to semi-automate dried blood spot (DBS) extraction of alprazolam, α-hydroxyalprazolam and midazolam from human whole blood. Liquid-handling devices were used to add internal standard to the DBS cards and to extract the analytes from the DBS, in order to be analyzed by HPLC-MS/MS.


RESULTS
The technique was shown to be accurate (±12.0%) and precise (10.3%) across the dynamic range of the assay.


CONCLUSIONS
The semi-automated extraction reduced sample preparation time by more than 50% when compared with more conventional DBS manual extraction methods.
Alturas Analytics, Inc., 1324 Alturas Drive, Moscow, ID 83843, USA. cjohnson@alturasanalytics.com
Casey Jl
CJ
Johnson
Chad D
CD
Christianson
Chrystal N
CN
Sheaff
Derek F
DF
Laine
Jennifer Sd
JS
Zimmer
Shane R
SR
Needham
D000525:Alprazolam / Q000031:analogs & derivatives / Q000097:blood; D001331:Automation; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008874:Midazolam / Q000097:blood; D053719:Tandem Mass Spectrometry
C032318:alpha-hydroxyalprazolam; D008874:Midazolam; D000525:Alprazolam