[Construction of expression vector driven by tobacco root-specific promoter and transformation into Rhodiola hairy root].
OBJECTIVE
To construct root-specific promoter of plant expression vector and transformate into Rhodiola hairy root.


METHODS
The expression vector pCA-Tob7: UGTR, driven by tobacco root-specific promoter TobRB7, was constructed from pCAM-BIA1301 by substituting the CaMV 35S promoter and GUS gene with TobRB7 and UGTR(a glycosyltransferase gene), respectively. The pCA-Tob7: UGTR vector was introduced into Agrobacterium tumefaciens strain, and the hairy root of Rhodiola were then transformed.


RESULTS
Some Kanamycin resistant plants were positive,which indicated that the expression vector had integrated into Rhodiola hairy root genome.


CONCLUSIONS
Root-specific promoter of plant expression vector is constructed and the hairy root of Rhodiola are transformed for future research.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China. huyaohui@vip.163.com
Yao-hui
YH
Hu
Bei-bei
BB
Liu
Han-song
HS
Yu
D060054:Agrobacterium; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D029771:Rhodiola / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D005964:Glucosyltransferases