Identification and characterization of a novel 21.6-kDa tegumental protein from Clonorchis sinensis.
Tegumental proteins form a membrane-bound outer surface and are thus involved in host-parasite interactions and parasite survival. A complementary DNA clone encoding a novel 21.6-kDa tegumental protein (CsTegu21.6, accession number JF911532) was identified in a sequence library for the adult Clonorchis sinensis liver fluke. The complete coding sequence was 564 bp and encoded a protein of 188 amino acids. A BLASTX search revealed identities from 43 to 47% with previously identified tegumental proteins in C. sinensis and other helminthic parasites. Multiple alignment of the amino acids of CsTegu21.6 with those of four other C. sinensis tegumental proteins, CsTegu21.1, CsTegu22.3, CsTegu20.8 and CsTegu31.8, revealed pair-wise sequence identities ranging from 24 to 31.8%. A calcium-binding EF-hand domain containing a basic helix-loop-helix structure at the N terminus and a dynein light chain domain at the C terminus were found in CsTegu21.6; these motifs are common in tegumental proteins. CsTegu21.6 was specifically observed on the tegument of adult worms using immunolocalization analysis.
Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Osong, 363-951, Republic of Korea. hoiyui25@gmail.com
Yu-Jung
YJ
Kim
Won Gi
WG
Yoo
Myoung-Ro
MR
Lee
Dae-Won
DW
Kim
Won-Ja
WJ
Lee
Jung-Mi
JM
Kang
Byoung-Kuk
BK
Na
Jung-Won
JW
Ju
D020816:Amino Acid Motifs; D000818:Animals; D003004:Clonorchis sinensis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D015801:Helminth Proteins; D008565:Membrane Proteins