Type I interferon signature is high in lupus and neuromyelitis optica but low in multiple sclerosis.
OBJECTIVE
Neuromyelitis optica (NMO) is characterized by selective inflammation of the spinal cord and optic nerves but is distinct from multiple sclerosis (MS). Interferon (IFN)-β mitigates disease activity in MS, but is controversial in NMO, with a few reports of disease worsening after IFN-β therapy in this highly active disease. In systemic lupus erythematosus (SLE), IFNs adversely affect disease activity. This study examines for the first time whether serum IFN-α/β activity and IFN-β-induced responses in peripheral blood mononuclear cells (MNC) are abnormally elevated in NMO, as they are in SLE, but contrast to low levels in MS.


METHODS
Serum type I IFN-α/β activity was measured by a previously validated bioassay of 3 IFN-stimulated genes (RT-PCR sensitivity, 0.1 U/ml) rather than ELISA, which has lower sensitivity and specificity for measuring serum IFNs. IFN responses in PBMNC were assessed by in vitro IFN-β-induced activation of phospho-tyrosine-STAT1 and phospho-serine-STAT1 transcription factors, and MxA proteins using Western blots.


RESULTS
Serum IFN-α/β activity was highest in SLE patients, followed by healthy subjects and NMO, but was surprisingly low in therapy-naïve MS. In functional assays in vitro, IFN-β-induced high levels of P-S-STAT1 in NMO and SLE, but not in MS and controls. IFN-β-induced MxA protein levels were elevated in NMO and SLE compared to MS.


CONCLUSIONS
Serum IFN activity and IFN-β-induced responses in PBMNC are elevated in SLE and NMO patients versus MS. This argues for similarities in pathophysiology between NMO and SLE and provides an explanation for IFN-induced disease worsening in NMO.
Department of Neurology, University of Chicago, Chicago, IL 60637, USA.
Xuan
X
Feng
Nicholas P
NP
Reder
Mounica
M
Yanamandala
Addie
A
Hill
Beverly S
BS
Franek
Timothy B
TB
Niewold
Anthony T
AT
Reder
Adil
A
Javed
D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000097:blood* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000175:diagnosis; D009471:Neuromyelitis Optica / Q000097:blood* / Q000175:diagnosis; D015854:Up-Regulation / Q000502:physiology
D015415:Biomarkers; D007370:Interferon Type I