An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts.
The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.
Positivo University, Curitiba, PR, Brazil.
Allan Fernando
AF
Giovanini
Denise Piotto
DP
Leonardi
Flares
F
Baratto-Filho
Paola Cristine
PC
Vlença
Ricardo César
RC
Moresca
Alexandre
A
Moro
Celso Alfredo
CA
Schramm
D000199:Actins / Q000032:analysis; D000818:Animals; D003094:Collagen / Q000032:analysis; D004853:Epoxy Resins / Q000633:toxicity; D005355:Fibrosis; D006097:Granulation Tissue / Q000473:pathology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry; D007249:Inflammation; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D058628:Myofibroblasts / Q000187:drug effects* / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000633:toxicity*; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
C477053:Acroseal; D000199:Actins; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C486893:epiphany sealer; C534916:epoxy resin-based root canal sealer; C074417:Endo-Fill; D003094:Collagen