Cryopreservation of coconut (Cocos nucifera L.) zygotic embryos by vitrification.
The present study investigates the effect of preculture conditions, vitrification and unloading solutions on survival and regeneration of coconut zygotic embryos after cryopreservation. Among the seven plant vitrification solutions tested, PVS3 was found to be the most effective for regeneration of cryopreserved embryos. The optimal protocol involved preculture of embryos for 3 days on medium with 0.6 M sucrose, PVS3 treatment for 16 h, rapid cooling and rewarming and unloading in 1.2 M sucrose liquid medium for 1.5 h. Under these conditions, 70-80 survival (corresponding to size enlargement and weight gain) was observed with cryopreserved embryos and 20-25 percent of the plants regenerated (showing normal shoot and root growth) from cryopreserved embryos were established in pots.
Biotechnology Section, Central Plantation Crops Research Institute, Kerala, India.
K K
KK
Sajini
A
A
Karun
C H
CH
Amamath
F
F
Engelmann
D003059:Cocos / Q000196:embryology / Q000254:growth & development* / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D013395:Sucrose / Q000737:chemistry; D013997:Time Factors; D058989:Vitrification
D003451:Cryoprotective Agents; D003470:Culture Media; D013395:Sucrose; D005990:Glycerol; D004121:Dimethyl Sulfoxide