Lycorine reduces mortality of human enterovirus 71-infected mice by inhibiting virus replication.
Human enterovirus 71 (EV71) infection causes hand, foot and mouth disease in children under 6 years old and this infection occasionally induces severe neurological complications. No vaccines or drugs are clinical available to control EV71 epidemics. In present study, we show that treatment with lycorine reduced the viral cytopathic effect (CPE) on rhabdomyosarcoma (RD) cells by inhibiting virus replication. Analysis of this inhibitory effect of lycorine on viral proteins synthesis suggests that lycorine blocks the elongation of the viral polyprotein during translation. Lycorine treatment of mice challenged with a lethal dose of EV71 resulted in reduction of mortality, clinical scores and pathological changes in the muscles of mice, which were achieved through inhibition of viral replication. When mice were infected with a moderate dose of EV71, lycorine treatment was able to protect them from paralysis. Lycorine may be a potential drug candidate for the clinical treatment of EV71-infected patients.
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Laboratory Animal Science, CAMS & Comparative Medicine Centre, PUMC, Chao Yang Strict, Pan Jia Yuan Nan Li No.5, Beijing 100021, China.
Jiangning
J
Liu
Yajun
Y
Yang
Yanfeng
Y
Xu
Chunmei
C
Ma
Chuan
C
Qin
Lianfeng
L
Zhang
D047151:Amaryllidaceae Alkaloids / Q000008:administration & dosage*; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D029821:Enterovirus A, Human / Q000187:drug effects*; D004769:Enterovirus Infections / Q000188:drug therapy* / Q000401:mortality / Q000473:pathology; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010243:Paralysis / Q000517:prevention & control; D010617:Phenanthridines / Q000008:administration & dosage*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012720:Severity of Illness Index; D016019:Survival Analysis; D014779:Virus Replication / Q000187:drug effects*
D047151:Amaryllidaceae Alkaloids; D000998:Antiviral Agents; D010617:Phenanthridines; D012367:RNA, Viral; C015330:lycorine