Synaptosomal protein synthesis in P2 and Ficoll purified fractions.
Cytoplasmic protein synthesis of brain synaptosomes has generally been determined in the Ficoll purified fraction which contains fewer contaminating mitochondria, microsomes and myelin fragments than the parent P2 fraction. Using a highly selective assay of this activity we have compared the total translation activity and the specific activity of the proteins synthesized by either fraction in control rats and in rats trained for a two-way active avoidance task. In control rats the specific activity remained essentially the same in both fractions but in trained rats the value of the Ficoll fraction was markedly lower (38.5%) than in the P2 fraction. Furthermore, the total translation activity of the Ficoll fraction was 30% lower than in the P2 fraction in control rats and 62% lower in trained rats. These decrements indicate that a large proportion of active synaptosomes present in the P2 fraction is not recovered in the Ficoll fraction, notably in rats undergoing plastic brain changes. We conclude that cytoplasmic protein synthesis of brain synaptosomes is better preserved in the P2 fraction.
Department of Biological Sciences, University of Naples 'Federico II', Via Mezzocannone 8, Naples 80134, Italy.
Maria
M
Eyman
Carolina
C
Cefaliello
Anna Paola
AP
Bruno
Annapaola
A
Bruno
Marianna
M
Crispino
Antonio
A
Giuditta
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002458:Cell Fractionation; D005362:Ficoll / Q000737:chemistry / Q000302:isolation & purification; D008297:Male; D018996:Myelin P2 Protein / Q000096:biosynthesis* / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D018996:Myelin P2 Protein; D005362:Ficoll