Evaluation of genotoxicity after application of Listerine(R) on human lymphocytes by micronucleus and single cell gel electrophoresis assays.
Listerine (LN) is one of the most commonly used mouth rinses worldwide although very limited information is available concerning its genotoxicity. In another view, the biological safety profile of oral care products is frequently assumed on the basis of simplistic test models. Therefore, the present study was undertaken to investigate the in vitro genotoxic potential of LN using micronucleus and single cell gel electrophoresis tests as genetic endpoints. Different concentrations of LN (0-100% of ml/culture, v/v) were applied to whole human blood cultures (n = 5). The result of the present study showed that there were no statistically significant differences (p > 0.05) between the control group and the groups treated with LN alone in both analysed endpoints. In conclusion, our result first demonstrated the absence of genotoxicity of LN on human lymphocytes.
Faculty of Science, Department of Biology, Atatürk University, Erzurum, Turkey.
Hasan
H
Türkez
Basak
B
Togar
Taner
T
Arabaci
D000704:Analysis of Variance; D002478:Cells, Cultured; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D012459:Salicylates / Q000633:toxicity*; D013729:Terpenes / Q000633:toxicity*
D004338:Drug Combinations; D012459:Salicylates; D013729:Terpenes; C057473:Listerine