Domain-based assays of individual antibody concentrations in an oligoclonal combination targeting a single protein.
Quantitation of individual monoclonal antibodies (mAbs) within a combined antibody drug product is required for preclinical and clinical drug development, including pharmacokinetic (PK), toxicology, stability, and biochemical characterization studies of such drugs. We have developed an antitoxin, XOMA 3AB, consisting of three recombinant mAbs that potently neutralize the known subtypes of type A botulinum neurotoxin (BoNT/A). The three mAbs bind nonoverlapping BoNT/A epitopes with high affinity. XOMA 3AB is being developed as a treatment for botulism resulting from BoNT/A. To develop antibody-specific assays, we cloned, expressed, and purified BoNT/A domains from Escherichia coli. Each mAb bound only to its specific domain with affinity comparable to the binding to holotoxin. mAb-specific domains were used to develop an enzyme-linked immunosorbent assay (ELISA) for characterization of the integrity and binding activity of the three mAbs in the drug product. An electrochemiluminescence bridging assay that is robust to interference from components in serum was also developed, and we demonstrate that it can be used for PK assays. This type of antigen engineering to generate mAb-specific domains is a general method allowing quantitation and characterization of individual mAbs in a mAb cocktail that binds the same protein and is superior to anti-idiotype approaches.
Department of Anesthesia, University of California, San Francisco, San Francisco General Hospital, San Francisco, CA 94110, USA.
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Q
Meng
M
M
Li
M A
MA
Silberg
F
F
Conrad
J
J
Bettencourt
R
R
To
C
C
Huang
J
J
Ma
K
K
Meyer
R
R
Shimizu
L
L
Cao
M T
MT
Tomic
J D
JD
Marks
D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000032:analysis / Q000276:immunology; D019274:Botulinum Toxins, Type A / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D019274:Botulinum Toxins, Type A