Searching for the protein targets of bioactive molecules.
The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal physiologically relevant interactions. Here we review a recently introduced yeast-based approach for the identification of the binding partners of small molecules. We discuss the advantages and limitations of the approach using the clinically approved drug sulfasalazine as an example.
Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne.
Christopher
C
Chidley
Hirohito
H
Haruki
Miriam Grønlund
MG
Pedersen
Cindy
C
Fellay
Simone
S
Moser
Kai
K
Johnsson
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001708:Biopterins / Q000031:analogs & derivatives / Q000096:biosynthesis; D001711:Biotransformation; D055808:Drug Discovery / Q000379:methods* / Q000639:trends; D015394:Molecular Structure; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012460:Sulfasalazine / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011506:Proteins; D054852:Small Molecule Libraries; D001708:Biopterins; D012460:Sulfasalazine; C003402:sapropterin