Impact of skeletal maturation on bone metabolism biomarkers and bone mineral density in healthy Brazilian male adolescents.
OBJECTIVE
To evaluate the behavior of biomarkers of bone formation and resorption in healthy male Brazilian adolescents according to their biological maturation.


METHODS
Eighty-seven volunteers were divided into age groups according to bone age (BA): 10-12 years (n = 25), 13-15 years (n = 36), and 16-18 years (n = 26). Weight (kg), height (m), body mass index (kg/m(2)), calcium intake from 3 days assessed by 24-h food recall (mg/day), pubertal event evaluation by Tanner criteria, and serum biomarker levels (osteocalcin [OC] [ng/mL], bone alkaline phosphatase [BAP] [U/L], and serum carboxyterminal telopeptide [S-CTx] [ng/mL]) were recorded and correlated to bone mineral density (BMD) (g/cm(2)) measured by dual energy X-ray absorptiometry of the lumbar spine, proximal femur, and whole body.


RESULTS
Biomarkers showed similar behaviors, presenting higher median values in the 13-15 year group (BAP = 154.71 U/L, OC = 43.0 ng/mL, S-CTx = 2.09 ng/mL; p < 0.01) and when adolescents were in the pubertal stage G4. Median biomarker values decreased with advancing BA and sexual maturation. Biomarker values showed parallelism with peak height velocity, and, interestingly, bone formation biomarkers indicated significant negative correlation with BMD in the different evaluated locations, i.e., higher BMD values correlated with lower bone biomarker values.


CONCLUSIONS
This is the first study of healthy Brazilian adolescents with rigid and careful inclusion and exclusion criteria to assess the correlation of bone markers and BMD with biological maturation indicators. Our results can help understand bone turnover and monitor bone metabolism.
Department of Physical Education, Universidade Estadual do Norte do Paraná, Jacarezinho, PR, Brazil.
Carla C
CC
Silva
Tamara B L
TB
Goldberg
Hong S
HS
Nga
Cilmery S
CS
Kurokawa
Renata C
RC
Capela
Altamir S
AS
Teixeira
José C
JC
Dalmas
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D015519:Bone Density / Q000502:physiology*; D001862:Bone Resorption / Q000097:blood; D001842:Bone and Bones / Q000378:metabolism*; D001938:Brazil; D024042:Collagen Type I / Q000097:blood*; D003430:Cross-Sectional Studies; D006801:Humans; D008297:Male; D015675:Osteocalcin / Q000097:blood*; D010012:Osteogenesis / Q000502:physiology; D010455:Peptides / Q000097:blood*; D012741:Sexual Maturation / Q000502:physiology*; D018709:Statistics, Nonparametric
D015415:Biomarkers; D024042:Collagen Type I; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; D000469:Alkaline Phosphatase