Diabetic nephropathy-related active cyclic peptides from the roots of Brachystemma calycinum.
Three new cyclic peptides, namely duanbanhuains A-C (1-3), were isolated from the roots of Brachystemma calycinum which is a traditional medicine used to treat rheumatic diseases. Their structures were identified by means of a suite of MS and NMR experiments. These compounds were purposely evaluated for their inhibitory effects on the release of MCP-1, IL-6, collagen IV and reactive oxygen species (ROS) against high-glucose-stimulated mesangial cells. The results showed that compounds 1 and 2 exhibited potent inhibition on the production of IL-6, collagen IV and ROS at the concentration of 10 μM.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China. yxcheng@mail.kib.ac.cn
Yong-Xian
YX
Cheng
Li-Li
LL
Zhou
Yong-Ming
YM
Yan
Ke-Xin
KX
Chen
Fan-Fan
FF
Hou
D000595:Amino Acid Sequence; D029748:Caryophyllaceae / Q000737:chemistry*; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D003928:Diabetic Nephropathies; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011487:Protein Conformation; D017382:Reactive Oxygen Species / Q000378:metabolism; D054390:Receptors, CCR2 / Q000378:metabolism
D024141:Collagen Type IV; D015850:Interleukin-6; D010456:Peptides, Cyclic; D017382:Reactive Oxygen Species; D054390:Receptors, CCR2; C570583:duanbanhuain A; C570584:duanbanhuain B; D005947:Glucose