Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic.
Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.
Laboratory of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
Megumi
M
Fujita
Hirotomo
H
Kato
Abraham G
AG
Cáceres
Eduardo A
EA
Gomez
Lenin
L
Velez
Tatsuyuki
T
Mimori
Feifei
F
Zhang
Hiroyuki
H
Iwata
Masataka
M
Korenaga
Tatsuya
T
Sakurai
Ken
K
Katakura
Yoshihisa
Y
Hashiguchi
D000818:Animals; D019353:Endemic Diseases; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007896:Leishmaniasis / Q000453:epidemiology*; D058889:Molecular Typing; D010568:Peru; D012150:Polymorphism, Restriction Fragment Length; D011576:Psychodidae / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D012337:RNA, Ribosomal, 18S