Cardiomyocytes in vitro adhesion is actively influenced by biomimetic synthetic peptides for cardiac tissue engineering.
Scaffolds for tissue engineering must be designed to direct desired events such as cell attachment, growth, and differentiation. The incorporation of extracellular matrix-derived peptides into biomaterials has been proposed to mimic biochemical signals. In this study, three synthetic fragments of fibronectin, vitronectin, and stromal-derived factor-1 were investigated for the first time as potential adhesive sequences for cardiomyocytes (CMs) compared to smooth muscle cells. CMs are responsive to all peptides to differing degrees, demonstrating the existence of diverse adhesion mechanisms. The pretreatment of nontissue culture well surfaces with the (Arginine-Glycine-Aspartic Acid) RGD sequence anticipated the appearance of CMs' contractility compared to the control (fibronectin-coated well) and doubled the length of cell viability. Future prospects are the inclusion of these sequences into biomaterial formulation with the improvement in cell adhesion that could play an important role in cell retention during dynamic cell seeding.
Department of Cardiac, Thoracic and Vascular Science, University of Padova, Padova, Italy. alessandro.gandaglia@unipd.it
Alessandro
A
Gandaglia
Rocio
R
Huerta-Cantillo
Marina
M
Comisso
Roberta
R
Danesin
Francesca
F
Ghezzo
Filippo
F
Naso
Alessandra
A
Gastaldello
Eleonora
E
Schittullo
Edward
E
Buratto
Michele
M
Spina
Gino
G
Gerosa
Monica
M
Dettin
D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D023822:Tissue Engineering / Q000379:methods*
D010455:Peptides