Preparation and characterization of chitosan-polyvinyl alcohol blend hydrogels for the controlled release of nano-insulin.
Chitosan (CS)-polyvinyl alcohol (PVA) blend hydrogels were prepared using glutaraldehyde as the cross-linking agent. The obtained hydrogels, which have the advantages of both PVA and CS, can be used as a material for the transdermal drug delivery (TDD) of insulin. The nano-insulin-loaded hydrogels were prepared under the following conditions: 1.2g of polyethylene glycol, 1.5 g of CS, 1.2 g of PVA, 1.2 mL of 1% glutaraldehyde solution, 16 mL of water, and 40 mg of nano-insulin with 12 min of mixing time and 3 min of cross-linking time. The nano-insulin-loaded hydrogels were characterized using scanning electron microscopy, energy dispersive spectrometry, Fourier-transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, and its mechanical properties were analyzed. The results show that all molecules in the hydrogel have good compatibility and they formed a honeycomb-like structure. The hydrogel also showed good mechanical and thermal properties. The in vitro drug release of the hydrogel showed that the nano-insulin accorded with Fick's first law of diffusion and it has a high permeation rate (4.421 μg/(cm(2)h)). These results suggest that the nano-insulin-loaded hydrogels are a promising non-invasive TDD system for diabetes chemotherapy.
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, Heilongjiang, China. zygorl@yahoo.com.cn
Yuangang
Y
Zu
Ying
Y
Zhang
Xiuhua
X
Zhao
Chang
C
Shan
Shuchong
S
Zu
Kunlun
K
Wang
Yong
Y
Li
Yunlong
Y
Ge
D002152:Calorimetry, Differential Scanning / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003920:Diabetes Mellitus / Q000188:drug therapy; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D036103:Nanotechnology / Q000379:methods; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013718:Tensile Strength; D013818:Thermogravimetry / Q000379:methods
D003432:Cross-Linking Reagents; D004337:Drug Carriers; D020100:Hydrogels; D007328:Insulin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011142:Polyvinyl Alcohol; D048271:Chitosan; D005976:Glutaral