[Quantitative analysis of hinokiol from cell suspension cultures of Cephalotaxus fortunei].
A high performance liquid chromatography (HPLC) method was developed for the separation of secondary metabolites and quantitative analysis of hinokiol from cell suspension cultures of Cephalotaxus fortunei. The samples were prepared by extraction using methanol followed by partitioning between ammonium hydroxide and chloroform. The HPLC separation was achieved on an Apollo C18 column (250 mm x 4.6 mm, 5 microm) with gradient elution using methanol and water at 1 mL/min and 30 degrees C. The detection was carried out at 290 nm. A good linear correlation between the hinokiol peak area and mass concentration was observed over the mass concentration range of 0.012 5 - 0.2 g/L. The proposed method was applied to the determination of hinokiol in the actual samples with recoveries of 87.2% - 94.7% and with the relative standard deviations of 0.9% - 4.2%. This method is reliable and reproducible and is suitable for the analysis of hinokiol in plant cell cultures.
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Science Dalian 116023, China.
Xiaohui
X
Xu
Wei
W
Zhang
Changhong
C
Yao
Xupeng
X
Cao
Song
S
Xue
D045784:Abietanes / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D002478:Cells, Cultured; D032483:Cephalotaxus / Q000737:chemistry* / Q000166:cytology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D046508:Culture Techniques / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D059828:Plant Cells / Q000378:metabolism
D045784:Abietanes; D004365:Drugs, Chinese Herbal; C000610513:hinokiol; C497963:seco-Hinokiol