Competitive ELISA for the detection of antibodies to Rift Valley fever virus in goats and cattle.
Rift Valley fever virus (RVFV) is one of the important emerging viral diseases of serious impact in public health and animal hygiene both in human and animal industries. In this study, we developed a monoclonal antibody-based competitive ELISA for the detection of antibodies to RVFV in goats and cattle. The recombinant N protein of RVFV was expressed in E. coli with a six-histidine tag, and the purified N protein was used for detecting antigen with a competitive monoclonal antibody against RVFV antibodies. The competitive ELISA (C-ELISA) could detect antibodies at 9-11 days after inoculation in goats and cattle with a sensitivity of 94.7% (virus neutralization titer >32) and specificity of 99.7%, respectively. In addition, the C-ELISA did not show any cross-reactivity with positive sera against arboviruses such as Akabane, Aino, Chuzan, Ibaraki and bovine ephemeral fever virus, which are prevalent viral agents in ruminant animals throughout Southeast Asia. The results of the present study indicate that the C-ELISA is a simple, rapid and convenient serodiagnostic method for RVFV in goats and cattle.
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 430–824, Republic of Korea.
Hyun-Joo
HJ
Kim
Jin-Ju
JJ
Nah
Jin-San
JS
Moon
Young-Joon
YJ
Ko
Han-Sang
HS
Yoo
Chang-Hee
CH
Kweon
D000818:Animals; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015967:Gene Expression Regulation, Viral; D015511:Goat Diseases / Q000175:diagnosis* / Q000821:virology; D006041:Goats; D012295:Rift Valley Fever / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012296:Rift Valley fever virus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014764:Viral Proteins