Production of monoclonal antibody to herbicide fenoxaprop-ethyl.
Fenoxaprop-ethyl is a selective aryloxyphenoxypropionate herbicide used widely to control annual and perennial grasses. A monoclonal antibody (MAb) against fenoxaprop-ethyl (FE), designated as 3E6B9C, was produced and had very low cross-reactivity with some of its structural analogs, such as clodinafop-propargyl, diclofop-methyl, lactofen, and quizalofop-p-ethyl. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed. The concentration of R-(+)-fenoxaprop-ethyl (R-FE) producing 50% of inhibition (IC(50)) and the working range of icELISA were 3.1 ng/mL and 0.6-29 ng/mL, respectively. This assay is also sensitive to R-fenoxaprop, S-(-)-fenoxaprop-ethyl, and metamifop with IC(50) of 3.4, 2.7, and 3.5 ng/mL, respectively. The recoveries of R-FE in soil samples with the icELISA were 86-102%.
College of Science, China Agricultural University, Beijing, People's Republic of China.
Yongliang
Y
Cui
Tiegui
T
Nan
Guiyu
G
Tan
Qing X
QX
Li
Baomin
B
Wang
Shangzhong
S
Liu
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis*; D000915:Antibody Affinity; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D006540:Herbicides / Q000737:chemistry / Q000276:immunology*; D006868:Hydrolysis; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology*; D010080:Oxazoles / Q000737:chemistry / Q000276:immunology*; D011422:Propionates / Q000737:chemistry / Q000276:immunology*; D012989:Soil Pollutants / Q000737:chemistry / Q000276:immunology*; D017186:Titrimetry
D058846:Antibodies, Monoclonal, Murine-Derived; D006241:Haptens; D006540:Herbicides; D010080:Oxazoles; D011422:Propionates; D012989:Soil Pollutants; C416661:fenoxaprop ethyl; D010047:Ovalbumin