Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation.
OBJECTIVE
After immediate teeth separation, expression of HSP27 in the mouse dental pulp was examined. Immunohistochemistry was performed to examine the incidence of HSP27 expression.


METHODS
A total of 36 8-week-old ddY mice were used as experimental subjects and a wedge was inserted in between maxillary right molars. The wedge was removed 30 min or 3 h after insertion. Animals were immediately sacrificed after the removal of wedge or until 1 week later and serial sections from paraffin-embedded tissues were prepared. Immunohistochemistry was carried out to examine the expression of HSP27. The untreated side served as the control.


RESULTS
In the control group, the endothelial cells and some pulp fibroblasts weakly expressed HSP27 suggesting that the expression is due to mechanical stress brought about by physiological masticatory force and pressure from the tongue. In both 30 min and 3 h experimental groups, HSP27 expression was highest at 24 h after wedge removal and the expression remained the same or started to decrease thereafter. The expression decreased at the same level as that of the control group 1 week after wedge removal.


CONCLUSIONS
HSP27 may serve as an indicator of stimulus strong enough to show its expression.
Matsumoto Dental University, Graduate School of Oral Medicine, Shiojiri, Japan.
S
S
Saito
K
K
Nakano
A
A
Nabeyama
M
M
Sato
N
N
Okafuji
A
A
Yamamoto
E
E
Kasahara
Toshiyuki
T
Kawakami
D000818:Animals; D003782:Dental Pulp / Q000378:metabolism* / Q000473:pathology*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D013314:Stress, Mechanical; D014070:Tooth / Q000378:metabolism* / Q000473:pathology*
D055551:HSP27 Heat-Shock Proteins; C486699:Hspb2 protein, mouse