The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
The neurotrophin family of proteins promote the survival and differentiation of nerve cells and are thought to play an important role in development of reproductive tissues. The objective of the present study was to detect the presence of Brain-derived neurotrophic factor (BDNF) and its receptor TrkB in bovine sperm, and explore the potential role of BDNF in sperm function. We demonstrated that both the neorotrophin BDNF and the tyrosine kinase receptor protein TrkB were expressed in ejaculated bovine sperm. Furthermore, BDNF per se was secreted by sperm. Insulin and leptin secretion by bovine sperm were increased (P < 0.01) when cells were exposed to exogenous BDNF, whereas insulin was decreased by K252a. Therefore, we inferred that BDNF could be a regulator of sperm secretion of insulin and leptin through the TrkB receptor. Sperm viability and mitochondrial activity were both decreased (P < 0.05) when the BDNF/TrkB signaling pathway was blocked with K252a. Furthermore, BDNF promoted apoptosis of bovine sperm through TrkB binding (P < 0.05). In conclusion, these observations provided evidence that BDNF secreted by bovine sperm was important in regulation of insulin and leptin secretion in ejaculated bovine sperm. Furthermore, BDNF may affect sperm mitochondrial activity and apoptosis, as well as their viability.
College of Animal Science and Veterinary Medicine and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, Jilin Province, PR of China.
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Li
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Li
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Zhu
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Wang
Zhuo
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Liu
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Li
Chen
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Lu
Xu
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Zhou
D000818:Animals; D017209:Apoptosis; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism*; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism* / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor; D020813:Receptor, trkB