Monitoring the active transport of efflux pumps after their reconstitution into proteoliposomes: caveats and keys.
There is an acute need for a functional assay allowing the investigation of efflux pumps. A dedicated procedure was previously developed, but although it was unambiguous, it suffered from a lack of reproducibility. We describe an optimization of the procedure that makes the assay much more robust.
Laboratoire de Cristallographie et RMN Biologiques (CNRS UMR 8015), Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité 75006 Paris, France. martin.picard@parisdescartes.fr
M
M
Picard
A
A
Verchère
I
I
Broutin
D001190:Arylsulfonates / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D001692:Biological Transport / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D011510:Proteolipids / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D001190:Arylsulfonates; D008081:Liposomes; D011510:Proteolipids; C020485:proteoliposomes; D014634:Valinomycin; C005047:pyranine