Influence of the activation mode of a self-etch resin-based luting cement upon the metabolism of odontoblast-like cells.
OBJECTIVE
To evaluate the cytotoxicity of a self-etch resin-based luting cement, RelyXUnicem (RXU) upon chemical or dual cure and with or without interposition of IPS d.SIGN (IPSD) or IPS Empress II (IPSE) ceramic discs between cement and light source.


METHODS
112 RXU specimens were subjected to different curing conditions and incubated in culture medium (DMEM) to obtain extracts. The following groups were formed: G1: DMEM (control); G2: dual RXU; G3: chemical RXU; G4: dual RXU+IPSD; G5: chemical RXU+IPSD; G6: dual RXU+IPSE; and G7: chemical RXU+IPSE. Cultured odontoblast-like cells were incubated for 24 hours in contact with the extracts. Data from cell metabolism (CM), total protein dosage (TPD) and alkaline phosphatase activity (APA) were obtained and analyzed statistically (alpha = 0.05; Kruskal Wallis and Mann-Whitney tests). Cell morphology was analyzed by SEM.


RESULTS
CM and APA were significantly lower in G3 and G7 than in G1 (P<0.05). Significant TPD decrease occurred in G5 and G7 compared to G1 (P<0.05). Only G4 and G6 presented CM changes. RXU caused no cytotoxicity when subjected to dual cure without ceramic interposition. However, mild cytopathic effects were observed after chemical setting without ceramic interposition, and after chemical and dual activation under ceramic discs.
Federal University of Alfenas, Minas Gerais, Brazil.
Adriano Mielo
AM
de Mendonça
Camila Favero
CF
de Oliveira
Josimeri
J
Hebling
Carlos Alberto de Souza
CA
Costa
D000469:Alkaline Phosphatase / Q000032:analysis; D001031:Apatites / Q000737:chemistry; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003776:Dental Porcelain / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D006801:Humans; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D018020:Lithium Compounds / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D019279:Resin Cements / Q000633:toxicity*; D055116:Self-Curing of Dental Resins / Q000379:methods; D013385:Succinate Dehydrogenase / Q000032:analysis; D013778:Tetrazolium Salts; D013844:Thiazoles
D001031:Apatites; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D017220:Dentin-Bonding Agents; C118557:Empress 2; D018020:Lithium Compounds; D011506:Proteins; C483701:Rely X Unicem; D019279:Resin Cements; D013778:Tetrazolium Salts; D013844:Thiazoles; C506670:d.SIGN ceramic; D003776:Dental Porcelain; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; C022616:thiazolyl blue