Chitosan enhanced gene delivery of cationic liposome via non-covalent conjugation.
Two new types of stable ternary complexes were formed by mixing chitosan with DOTAP/pDNA lipoplex and DOTAP with chitosan/pDNA polyplex via non-covalent conjugation for the efficient delivery of plasmid DNA. They were characterized by atomic force microscopy, gel retarding, and dynamic light scattering. The DOTAP/CTS/pDNA complexes were in compacted spheroids and irregular lump of larger aggregates in structure, while the short rod- and toroid-like and donut shapes were found in CTS/DOTAP/pDNA complexes. The transfection efficiency of the lipopolyplexes showed higher GFP gene expression than DOTAP/pDNA and CTS/pDNA controls in Hep-2 and Hela cells, and luciferase gene expression 2-3-fold than DOTAP/pDNA control and 70-120-fold than CTS/pDNA control in Hep-2 cells. The intracellular trafficking was examined by confocal laser scanning microscopy. Rapid pDNA delivery to the nucleus enchanced by chitosan was achieved after 4 h transfection.
College of Environmental and Biological Science, Dalian University of Technology, Dalian, 116024, Liaoning, People's Republic of China.
Bing
B
Wang
Shubiao
S
Zhang
Shaohui
S
Cui
Baoling
B
Yang
Yinan
Y
Zhao
Huiying
H
Chen
Xiaomin
X
Hao
Qiong
Q
Shen
Jiti
J
Zhou
D002460:Cell Line; D048271:Chitosan / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D004847:Epithelial Cells; D018014:Gene Transfer Techniques; D022781:Hepatocytes; D006801:Humans; D008081:Liposomes / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000378:metabolism / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D010957:Plasmids / Q000378:metabolism*; D014162:Transfection
D004337:Drug Carriers; D008081:Liposomes; D046911:Macromolecular Substances; D004247:DNA; D048271:Chitosan