[Interleukin-4 and osteoprotegerin suppress polyethylene wear debris-induced osteolysis in a murine air pouch model].
OBJECTIVE
To test the effect of recombinant interleukin-4 (IL-4) and recombinant osteoprotegerin (OPG) in suppressing bone resorption induced by polyethylene wear particles..


METHODS
A cranial bone allograft was introduced into the air pouches induced on the back of BALB/c mice, followed by injection of 1 ml suspension of polyethylene particles into the pouches. The mouse models were then divided into 3 groups to receive injections of saline (control), IL-4 alone, or IL-4 and OPG into the pouches. The tissues were harvested 21 days after bone implantation for molecular and histological analyses.


RESULTS
Polyethylene wear particles-stimulated inflammatory responses (increased cellular infiltration and IL-1 and TNF production) were markedly reduced by IL-4 treatment either alone or combined with OPG (P<0.05). Polyethylene particles significantly increased tartrate-resistant acid phosphatase (TRAP) staining and bone absorption of the implanted bone graft, and IL-4 treatment, either alone or combined with OPG, obviously reduced the osteolysis induced by polyethylene particles (P<0.05).


CONCLUSIONS
IL-4 offers protection against polyethylene wear debris-induced inflammation and bone resorption in this mouse model. IL-4 combined with OPG can be a feasible and effective therapeutic approach to the treatment and prevention of polyethylene wear debris-associated osteolysis and aseptic loosening of the prosthetic components.
Department of Orthopedics, Chongqing Medical University, Chongqing, China. 19163564@qq.com
Yang
Y
Wang
Rui
R
Zhou
Ning-ning
NN
Wu
Yu-qin
YQ
Mou
Rui-dong
RD
Li
Zhong-liang
ZL
Deng
D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced / Q000517:prevention & control; D004195:Disease Models, Animal; D005260:Female; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009984:Orthopedic Fixation Devices / Q000009:adverse effects*; D010014:Osteolysis / Q000139:chemically induced / Q000517:prevention & control*; D053244:Osteoprotegerin / Q000494:pharmacology*; D020959:Polyethylene / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000494:pharmacology
D053244:Osteoprotegerin; D011994:Recombinant Proteins; D015847:Interleukin-4; D020959:Polyethylene