Inhibitory effect of YQHYRJ recipe on osteoblast differentiation induced by BMP-2 in fibroblasts from posterior longitudinal ligament of mice.
Ossification of posterior longitudinal ligament (OPLL) is a common disease in Asian countries. Osteoblast differentiation in posterior longitudinal ligamentous fibroblast is a pathologic basis of OPLL. Nowadays, an effective pharmacotherapy for OPLL is still hunted for. YQHYRJ Recipe (YQHYRJ) is designed based on traditional Chinese medicine (TCM) theories, and previous clinic trials reported its effect on relieving syndromes of cervical spondylopathy. To clarify the YQHYRJ effect of OPLL on a cellular level, we induced mice fibroblasts from posterior longitudinal ligaments to differentiate into osteoblasts by human recombinant BMP-2, and treated them with YQHYRJ and its three sub-compounds: YQ, HY and RJ. YQHYRJ and the sub-compounds reduced the increase of fibroblast proliferation, mineralization, type I collagen secretion induced by BMP-2 via MTT, alizarin red staining and immunochemical examination. Moreover, these agents inhibited BMP-2 induced upregulation of ossification-related genes ALP, Col I and OC as well as BMP signal molecules Smad1, Smad 5 and Runx2 mRNA expression. These results suggested YQHYRJ to be effective in inhibiting osteoblast differentiation induced by BMP-2 in fibroblasts from posterior longitudinal ligament. YQHYRJ might be a promising medicine for preventing OPLL disease.
Department of Orthopaedics & Traumatology, Longhua Hospital, Shanghai, China.
Qin
Q
Bian
Kuan
K
Jia
Shu-Fen
SF
Liu
Bing
B
Shu
Qian-Qian
QQ
Liang
Chong-Jian
CJ
Zhou
Quan
Q
Zhou
Yong-Jun
YJ
Wang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D024042:Collagen Type I / Q000378:metabolism; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D017846:Longitudinal Ligaments / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D017887:Ossification of Posterior Longitudinal Ligament / Q000378:metabolism / Q000473:pathology; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051898:Smad1 Protein / Q000096:biosynthesis; D051902:Smad5 Protein / Q000096:biosynthesis; D013778:Tetrazolium Salts; D013844:Thiazoles
D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D051898:Smad1 Protein; C497401:Smad1 protein, mouse; D051902:Smad5 Protein; C497798:Smad5 protein, mouse; D013778:Tetrazolium Salts; D013844:Thiazoles; D015675:Osteocalcin; D000469:Alkaline Phosphatase; C022616:thiazolyl blue