Antifungal susceptibilities of Aspergillus fumigatus clinical isolates obtained in Nagasaki, Japan.
We investigated the triazole, amphotericin B, and micafungin susceptibilities of 196 A. fumigatus clinical isolates in Nagasaki, Japan. The percentages of non-wild-type (non-WT) isolates for which MICs of itraconazole, posaconazole, and voriconazole were above the ECV were 7.1%, 2.6%, and 4.1%, respectively. A G54 mutation in cyp51A was detected in 64.2% (9/14 isolates) and 100% (5/5 isolates) of non-WT isolates for itraconazole and posaconazole, respectively. Amphotericin B MICs of ≥2 μg/ml and micafungin minimum effective concentrations (MECs) of ≥16 μg/ml were recorded for two and one isolates, respectively.
Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Masato
M
Tashiro
Koichi
K
Izumikawa
Asuka
A
Minematsu
Katsuji
K
Hirano
Naoki
N
Iwanaga
Shotaro
S
Ide
Tomo
T
Mihara
Naoki
N
Hosogaya
Takahiro
T
Takazono
Yoshitomo
Y
Morinaga
Shigeki
S
Nakamura
Shintaro
S
Kurihara
Yoshifumi
Y
Imamura
Taiga
T
Miyazaki
Tomoya
T
Nishino
Misuzu
M
Tsukamoto
Hiroshi
H
Kakeya
Yoshihiro
Y
Yamamoto
Katsunori
K
Yanagihara
Akira
A
Yasuoka
Takayoshi
T
Tashiro
Shigeru
S
Kohno
D019943:Amino Acid Substitution; D000666:Amphotericin B / Q000008:administration & dosage; D000935:Antifungal Agents / Q000008:administration & dosage*; D001228:Aspergillosis / Q000188:drug therapy* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000187:drug effects; D054714:Echinocandins / Q000008:administration & dosage; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D017964:Itraconazole / Q000008:administration & dosage; D007564:Japan; D055666:Lipopeptides / Q000008:administration & dosage; D008297:Male; D000077551:Micafungin; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011743:Pyrimidines / Q000008:administration & dosage; D017422:Sequence Analysis, DNA; D014230:Triazoles / Q000008:administration & dosage; D065819:Voriconazole
D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D055666:Lipopeptides; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; C101425:posaconazole; D000666:Amphotericin B; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus; D065819:Voriconazole; D000077551:Micafungin