[Establishment of transgenic mice with visualized neovascularization].
OBJECTIVE
To establish transgenic mice with GFP expression in the vascular endothelium during neovascularization.


METHODS
The vector nestin-hsp68-gfp containing nestin second intron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry.


RESULTS
Thirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth.


CONCLUSIONS
The transgenic mice with GFP expression in the vascular endothelium  during neovascularization have been successfully established.
Department of Histology and Embryology, Southern Medical University, Guangzhou, China. zhenlin@fimmu.com
Zhen-lin
ZL
Li
Yi
Y
Gao
Jun-shuang
JS
Jia
Song-qin
SQ
Chen
Hai-hong
HH
Wang
Jing
J
An
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D004730:Endothelium, Vascular / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D064231:Nestin
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins