Short interference RNA introduced into cultured cells with diagnostic             ultrasound.
Diagnostic ultrasound (US) is safe. Short interference (si) RNA of Frizzled             (Fz)-9 suppresses proliferation of hepatocellular carcinoma cells. siRNA was introduced             into Hep3B cells, a human hepatocellular carcinoma cell line, with transfection             or US upward to culture plates at mechanical index (MI) = 0.4 or 0.8. siRNA of             Fz9 was introduced for western blot analysis and cell proliferation assay. siRNA             of Fz9 suppressed cell proliferation to 32.8±13.6% at 200 nM (P<0.0001) with             transfection. With US, cell proliferation decreased to 36.9±15.1% at 200 nM (P=0.039)             at 0.4 of MI while 49.2±12.0% at 200 nM (P=0.041) at MI=0.8. Western blot analysis             showed that 200 nM of siRNA decreased the expression of Fz9 with US at MI=0.4             and MI=0.8. No change was seen in cell proliferation between transfection and             US. siRNA was introduced into cultured cells and functioned with US.
Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, 934-5 Shikawatashi, Yotsukaido City, Chiba 284-0003, Japan. nihminor-cib@umin.ac.jp
Minoru
M
Tomizawa
Fuminobu
F
Shinozaki
Takao
T
Sugiyama
Shigenori
S
Yamamoto
Makoto
M
Sueishi
Takanobu
T
Yoshida
D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D045744:Cell Line, Tumor; D051157:Frizzled Receptors / Q000235:genetics; D018014:Gene Transfer Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D013016:Sound
C549560:FZD9 protein, human; D051157:Frizzled Receptors; D034741:RNA, Small Interfering