Feeding deterrent activity of synthesized silver nanoparticles using Manilkara zapota leaf extract against the house fly, Musca domestica (Diptera: Muscidae).
With a greater awareness of the hazards associated with the use of synthetic organic insecticides, there has been an urgent need to explore suitable alternative products for pest control. Musca domestica is ubiquitous insect that has the potential to spread a variety of pathogens to humans and livestock. They are mechanical carriers of more than hundred human and animal intestinal diseases and are responsible for protozoan, bacterial, helminthic, and viral infections. The present work aimed to investigate the feeding deterrent activity of synthesized silver nanoparticles (Ag NPs) using leaf aqueous extract of Manilkara zapota against M. domestica. The synthesized Ag NPs were recorded from UV-vis spectrum at 421 nm and scanning electron microscopy confirm the biosynthesis and characterization of Ag NPs with spherical and oval in shape and size of 70-140 nm. The FTIR analysis of the purified nanoparticles showed the presence of bands 1,079, 1,383, 1,627, 2,353, and 2,648 cm(-1), which were complete synthesis of AgNPs; the XRD pattern of AgNPs showed diffraction peaks at 2θ values of 38.06°, 44.37°, 64.51°, and 77.31° sets of lattice planes were observed (111), (200), (220), and (311) facts of silver, respectively. Adult flies were exposed to different concentrations of the aqueous extract of synthesized Ag NPs, 1 mM silver nitrate (AgNO(3)) solution and aqueous extract of M. zapota for 1, 2, and 3 h; however, AgNPs showed 72% mortality in 1 h, 89% mortality was found in 2 h, and 100% mortality was found in 3 h exposure at the concentration of 10 mg/mL and the leaf aqueous extract showed 32% mortality in 1 h, 48% mortality was found in 2 h, and 83% mortality was found in 3 h exposure at concentration of 50 mg/mL. The most efficient activity was observed in synthesized Ag NPs against M. domestica (LD(50) = 3.64 mg/mL; LD(90) = 7.74 mg/mL), the moderate activity reported in the aqueous extract of M. zapota (LD(50) = 28.35 mg/mL; LD(90) = 89.19 mg/mL) and nil activity were observed in AgNO(3) solution at 3 h exposure time at 10 mg/mL. Dimethyl 2, 2-dichlorovinyl phosphate (DDVP) was used as a positive control and showed the LD(50) value of 3.38 mL/L. These results suggest that the synthesized Ag NPs have the potential to be used as an ideal eco-friendly approach for the control of the adult of M. domestica. This method is considered as a new approach to control sanitary pest. Therefore, this study provides first report on the feeding deterrent activity of synthesized Ag NPs against housefly.
Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C Abdul Hakeem College, Melvisharam, 632 509 Vellore District, Tamil Nadu, India.
Chinnaperumal
C
Kamaraj
Govindasamy
G
Rajakumar
Abdul Abdul
AA
Rahuman
Kanayairam
K
Velayutham
Asokan
A
Bagavan
Abdul Abduz
AA
Zahir
Gandhi
G
Elango
D000818:Animals; D006793:Houseflies / Q000187:drug effects*; D007302:Insect Repellents / Q000378:metabolism / Q000494:pharmacology*; D007928:Lethal Dose 50; D032129:Manilkara / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D012834:Silver / Q000378:metabolism / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D016019:Survival Analysis
D007302:Insect Repellents; D010936:Plant Extracts; D012834:Silver