Modulation of mitochondrial respiratory capacity by carrier-mediated transfer of RNA in vivo.
Genetic dysfunction of mitochondria is pathological, but an effective method of nucleic acid delivery to mitochondria in vivo is lacking. Injection into rodents of tagged polycistronic RNAs (pcRNAs) encoding parts of the organelle genome and bound to a carrier complex, resulted in rapid uptake and concentration of the RNA in many tissues. The delivered RNA was localized to mitochondria. A pan-genomic cocktail of pcRNAs restored mRNA levels, stimulated mitochondrial translation and respiratory capacity of skeletal muscle of middle-aged and old rats. Thus, the carrier-based protocol may be suitable for delivery of functional RNAs to mitochondria in vivo.
Genetic Engineering Laboratory, Indian Institute of Chemical Biology (C.S.I.R), 4 Raja S. C. Mullick Road, Calcutta 700032, India.
Sukanta
S
Jash
Tapas
T
Chowdhury
Samit
S
Adhya
D000818:Animals; D019069:Cell Respiration / Q000187:drug effects*; D018014:Gene Transfer Techniques; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D009132:Muscles / Q000166:cytology; D012313:RNA / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014170:Transformation, Genetic
D012313:RNA