Design and characterization of a metal ion-imidazole self-assembled monolayer for reversible immobilization of histidine-tagged peptides.
We report the design and characterization of a metal ion-imidazole self-assembled monolayer on a gold electrode. The resultant monolayer is well-suited for direct immobilization of histidine and methylene blue-modified peptides.
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Anita J
AJ
Zaitouna
Rebecca Y
RY
Lai
D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
C471213:His-His-His-His-His-His; D007093:Imidazoles; D055767:Immobilized Proteins; D007477:Ions; D008670:Metals; D009842:Oligopeptides; D010455:Peptides; D006639:Histidine; D006046:Gold; C029899:imidazole; D008751:Methylene Blue