Influences of laparoscopic resection on expressions of metastasis genes in colorectal cancer: report of microarray analysis and literature review.
OBJECTIVE
To comprehensively understand the influence of laparoscopic resection on expressions of metastasis related genes in colorectal cancer by microarray.


METHODS
Adjacent cancer tissues were obtained from the same patient with rectal cancer pre and post laparoscopic resection. The mRNA expressions of metastasis related genes were detected by GEArray Q Series human tumor metastasis gene array HS-007. The microarray results were verified by real-time PCR. Preclinical studies focusing on the effect of CO2 pneumoperitoneum on colorectal cancer metastasis over the last ten years were reviewed.


RESULTS
Microarray analysis revealed that the expression of many metastasis related genes changed significantly post laparoscopic operation. Several metastatic mechanisms were activated including extracellular matrix degradation, cell adhesion, inflammation and angiogenesis. Meanwhile, some well known metastasis inhibition pathways were also activated, including NM23, PTEN and Thrombospondin. In the literature review there were inconsistent findings among reported experimental studies in terms of the effect of CO2 pneumoperitoneum on colorectal cancer metastasis.


CONCLUSIONS
This microarray finding implies that laparoscopic resection produced no overwhelming adverse effect on the expressions of metastasis related genes in colorectal cancer. In view of the increasing follow-up results from a number of clinical trials, laparoscopic surgery with CO2 pneumoperitoneum appears to be safe.
Department of Social Oncology, Zhejiang University College of Medicine, Hangzhou, China.
Jun
J
Li
Jian-Wei
JW
Wang
Shu
S
Zheng
Su-Zhan
SZ
Zhang
Ke-Feng
KF
Ding
D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics / Q000601:surgery*; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D008297:Male; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D011028:Pneumoperitoneum, Artificial