Lipid peroxidation in the first trimester of pregnancy.
OBJECTIVE
To determine the existence and level of oxidative stress caused by lipid peroxidation in pregnancy.


METHODS
The research was conducted as prospective examination that included 60 healthy women (age 18-45). The women included in the examination were divided into two groups. The group I (N-31) included women in the first trimester of normal, healthy pregnancy. The group II included healthy nongravid women (N-29). Concentrations of markers of lipid peroxidation malondialdehyde (MDA) and thiobarbituric acid (TBARS) were determined using commercial ELISA tests OxiSelect(™) TBARS Assay Kit and OxiSelect(™) MDA ELISA Kit.


RESULTS
The results of this research indicate that the concentrations of the markers of lipid peroxidation TBARS and MDA are detectable in both groups. Higher mean values of MDA (>20 pmol/mg) were measured in the group of pregnant women, than in the group of nongravid women. The results indicate that mean values of TBARS markers are lower in pregnant women (≤50 µM) than in nongravid women (>100 µM).


CONCLUSIONS
The marker of lipid peroxidation MDA proved to be a sensitive marker for following lipid peroxidation during pregnancy, therefore it can be considered as a good predictor of possible complications during pregnancy.
Department of obstetrics and gynecology, Clinical center Vojvodina, Branimira Cosica, Novi Sad, Serbia.
Branislava
B
Jakovljevic
Aleksandra
A
Novakov-Mikic
Snezana
S
Brkic
Mirjana A
MA
Bogavac
Slavica
S
Tomic
Vera
V
Miler
D000293:Adolescent; D000328:Adult; D015331:Cohort Studies; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000502:physiology; D011247:Pregnancy / Q000097:blood / Q000378:metabolism; D011248:Pregnancy Complications / Q000097:blood / Q000378:metabolism; D011261:Pregnancy Trimester, First / Q000097:blood / Q000378:metabolism*; D012907:Smoking / Q000097:blood / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D055815:Young Adult
D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde