Amelioration of renal ischemia-reperfusion injury with a novel protective cocktail.
OBJECTIVE
Extended warm ischemia during partial nephrectomy can lead to considerable renal injury. Using a rat model of renal ischemia we examined the ability of a unique renoprotective cocktail to ameliorate warm ischemia-reperfusion injury.


METHODS
A warm renal ischemia model was developed using 60 Sprague-Dawley® rats. The left renal artery was clamped for 40 minutes, followed by 48 hours of reperfusion. A renoprotective cocktail of a mixture of specific growth factors, mitochondria protecting biochemicals and Manganese-Porphyrin (MnTnHex-2-PyP(5+)) was given intramuscularly at -24, 0 and 24 hours after surgery. At 48 hours the 2 kidneys were harvested and examined with hematoxylin and eosin, and periodic acid-Schiff stains. Protein and gene expression were also analyzed to determine ischemia markers and the antioxidant response.


RESULTS
Compared to ischemic controls, kidneys treated with the renoprotective cocktail showed significant reversal of morphological changes and a significant decrease in the specific ischemic markers lipocalin-2, mucin-1 and galectin-3. Quantitative reverse transcriptase-polymerase chain reaction revealed up-regulation of several antioxidant genes in treated animals.


CONCLUSIONS
According to histopathological and several molecular measures our unique renoprotective cocktail mitigated ischemia-reperfusion injury.
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. thambi_dorai@nymc.edu
Thambi
T
Dorai
Andrew I
AI
Fishman
Cheng
C
Ding
Ines
I
Batinic-Haberle
David S
DS
Goldfarb
Michael
M
Grasso
D000818:Animals; D007668:Kidney / Q000098:blood supply*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*