A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation.
In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis(6)] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.
PHARM-REGION, Ltd, c.1, b.10, Baryshikha street, Moscow, 125222, Russia. smlk03@mail.ru
S P
SP
Klykov
V V
VV
Kurakov
V B
VB
Vilkov
I V
IV
Demidyuk
T Yu
TY
Gromova
D A
DA
Skladnev
D018929:Cell Culture Techniques / Q000379:methods*; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005818:Genetic Engineering; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D045726:Metalloproteases / Q000096:biosynthesis*; D008954:Models, Biological
D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D045726:Metalloproteases