Aerobic remediation of petroleum sludge through soil supplementation: microbial community analysis.
The effect of soil concentration on the aerobic degradation of real-field petroleum sludge was studied in slurry phase reactor. Total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAHs) showed effective removal but found to depend on the soil concentration. Aromatic fraction (48.12%) documented effective degradation compared to aliphatics (47.31%), NSO (28.69%) and asphaltenes (26.66%). PAHs profile showed efficient degradation of twelve individual aromatic compounds where lower ring compounds showed relatively higher degradation efficiency compared to the higher ring compounds. The redox behaviour and dehydrogenase activity showed a linear increment with the degradation pattern. Microbial community composition and changes during bioremediation were studied using denaturing gradient gel electrophoresis (DGGE). Among the 12 organisms identified, Proteobacteria was found to be dominant representing 50% of the total population (25% of γ-proteobacteria; 16.6% of β-proteobacteria; 8.3% of α-proteobacteria), while 33.3% were of uncultured bacteria and 16.6% were of firmicutes.
Bioengineering and Environmental Centre (BEEC), Indian Institute of Chemical Technology CSIR-IICT, Hyderabad, India.
M Venkateswar
MV
Reddy
M Prathima
MP
Devi
K
K
Chandrasekhar
R Kannaiah
RK
Goud
S Venkata
SV
Mohan
D000332:Aerobiosis; D001483:Base Sequence; D001673:Biodegradation, Environmental; D017931:DNA Primers; D052918:Environmental Restoration and Remediation / Q000379:methods*; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D011083:Polycyclic Compounds / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D012722:Sewage; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D017931:DNA Primers; D010578:Petroleum; D011083:Polycyclic Compounds; D012722:Sewage; D012989:Soil Pollutants