Exploration of a new concept for automated dried blood spot analysis using flow-through desorption and online SPE-MS/MS.
BACKGROUND
A new concept for simple and generic automation of dried blood spot (DBS) analysis is evaluated. Flow-through desorption of the blood spot is coupled online to SPE and MS/MS without using a LC column. Blood, spiked with a mixture of test compounds is spotted on paper, dried and then desorbed by means of a prototype clamping device.


RESULTS
Conditions for extraction and chromatography on a single SPE cartridge were optimized with respect to clean-up, separation and ionization suppression. Addition of internal standard using loop-injection upstream of the clamped blood spot was examined and proved to be simple and reliable. Validation results for the 1-1000 ng/ml range are well within acceptance criteria for bioanalysis.


CONCLUSIONS
Results demonstrate feasibility of the DBS SPE-MS/MS concept for efficient automation of the entire DBS analysis workflow.
Spark Holland B.V., Pieter de Keyserstraat 8, Emmen, The Netherlands. bert.ooms@sparkholland.com
Jan Albert
JA
Ooms
Lena
L
Knegt
Emile Hermannus Maarten
EH
Koster
D001331:Automation; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing; D006801:Humans; D007099:Imipramine / Q000097:blood; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014700:Verapamil / Q000097:blood
D014700:Verapamil; D007099:Imipramine