New technologies for studying the complexity of oral diseases.
Several new technologies are providing useful diagnostic tools and new information related to the pathogenesis of certain oral diseases. In this review, we describe several of these technologies including gene and microRNA arrays, proteomics, and antigen arrays as they relate to the study of Sjögren's syndrome and head and neck cancer. A common theme is the systematic analysis of large-scale inventories of RNAs, proteins, and autoantibody biomarkers revealing information not previously recognized. We also discuss metagenomic approaches that characterize the many different microorganisms present in the oral cavity that may impact oral and human health. Lastly, we describe applications of a new type of antibody-profiling technology termed Luciferase Immunoprecipitation Systems (LIPS), which has a wide dynamic range of detection of both linear and conformational epitopes needed for optimum diagnostics and biomarker discovery. We propose that the information offered by these technologies will enhance our ability to diagnose, treat, and further understand the pathogenesis of multiple oral diseases.
Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA. burbelop@nidcr.nih.gov
P D
PD
Burbelo
A
A
Bayat
E E
EE
Lebovitz
M J
MJ
Iadarola
D000906:Antibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D008156:Luciferases / Q000378:metabolism; D054892:Metagenome / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics; D025202:Molecular Diagnostic Techniques; D009055:Mouth / Q000382:microbiology; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000235:genetics
D000906:Antibodies; D015415:Biomarkers; D035683:MicroRNAs; D012471:Salivary Proteins and Peptides; D008156:Luciferases