Kinetic capillary electrophoresis with mass-spectrometry detection (KCE-MS) facilitates label-free solution-based kinetic analysis of protein-small molecule binding.
Tandem tracker: Here we introduce a method for studying the kinetics of protein-small-molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE-MS tandem is a highly versatile platform for label-free, solution-based kinetic studies of affinity interactions.
Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Ontario M3J 1P3, Canada.
Jiayin
J
Bao
Svetlana M
SM
Krylova
Derek J
DJ
Wilson
Oren
O
Reinstein
Philip E
PE
Johnson
Sergey N
SN
Krylov
D000526:Alprenolol / Q000737:chemistry / Q000378:metabolism; D019075:Electrophoresis, Capillary; D007700:Kinetics; D007741:Labetalol / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D009961:Orosomucoid / Q000737:chemistry / Q000378:metabolism; D010869:Pindolol / Q000737:chemistry / Q000378:metabolism; D011433:Propranolol / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000737:chemistry / Q000378:metabolism*
D009961:Orosomucoid; D011506:Proteins; D054852:Small Molecule Libraries; D000526:Alprenolol; D011433:Propranolol; D010869:Pindolol; D007741:Labetalol