Interaction between mannosylated lipoarabinomannan and dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin influences dendritic cells maturation and T cell immunity.
The aim of the study was to investigate the interaction between manLAM and DC-SIGN influencing DCs maturation and downstream immune response using small interfering RNA-expressing lentiviral vectors to specifically knockdown DC-SIGN. Our data indicated that DC-SIGN knockdown alone in DCs did not affect the maturation or the immunological function of lipopolysacharide (LPS)-activated DCs. Surface molecules were dramatically down-regulated in DCs primed with manLAM but not in mock control DCs (P<0.05). Meanwhile, manLAM enhanced the production of the immunosuppressive cytokine IL-10 in DCs (P<0.05). The level of IFN-γ was significantly down-regulated in the supernatants of naive T cells after co-cultured with DCs primed with manLAM (P<0.05). We demonstrated that DCs primed with manLAM may partially impair maturation phenotypes and immune response in LPS-activated DCs. However, the alterations of DCs function and downstream immune response caused by manLAM were reversed by the knockdown of DC-SIGN.
Department of Respiratory Medicine, The First Affiliated Hospital, Chongqing Medical University, No. 1 You Yi Road, Chongqing, China.
Tingting
T
Wu
Shuliang
S
Guo
Jianjun
J
Wang
Lan
L
Li
Lulu
L
Xu
Ping
P
Liu
Shuren
S
Ma
Jie
J
Zhang
Li
L
Xu
Yongai
Y
Luo
D015703:Antigens, CD / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects* / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D020868:Gene Silencing / Q000187:drug effects; D006031:Glycosylation; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism*; D016086:Lentivirus; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D008358:Mannose / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000276:immunology; D034741:RNA, Small Interfering / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D014162:Transfection; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D007371:Interferon-gamma; D016753:Interleukin-10; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008358:Mannose; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C050016:lipoarabinomannan