Purification of a new fungal mannose-specific lectin from Penicillium chrysogenum and its aphicidal properties.
Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on SDS-PAGE gel. Purified PeCL fraction was delivered via artificial diet to Myzus persicae aphid and was demonstrated to be aphicidal at 0.1 % with higher toxic efficiency than a known mannose-binding lectin Concanavalin A (ConA). A fast and efficient way to purify PeCL and a potential use in pest control is described.
Functional & Evolutionary Entomology, Gembloux Ago-Bio Tech, University of Liege, Passage des Déportés 2, B-5030 Gembloux, Belgium. Frederic.Francis@ulg.ac.be
Frédéric
F
Francis
Karimi
K
Jaber
Frédéric
F
Colinet
Daniel
D
Portetelle
Eric
E
Haubruge
D000818:Animals; D001042:Aphids / Q000187:drug effects*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D006386:Hemagglutination Tests; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D010408:Penicillium chrysogenum / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits
D005656:Fungal Proteins; D007306:Insecticides; D037241:Mannose-Binding Lectins