Human ether-à-go-go-related gene expression is essential for cisplatin             to induce apoptosis in human gastric cancer.
Human ether-à-go-go-related gene (HERG) is over-expressed in a wide range             of human cancers and regulates survival, migration, and apoptosis. The aim of             this study was to investigate the role of HERG in cisplatin-induced apoptosis             in gastric cancer in vitro and in vivo. siRNA was used to silence HERG expression.             HERG expression was detected by Western blot analysis in vitro, and further confirmed             by immunohistochemistry in vivo. Chemosensitivity to cisplatin in gastric cancer             cells was analyzed with a Cell Counting Kit-8 assay. Apoptosis was detected by             flow cytometry in vitro, and in situ apoptotic SGC7901 human gastric tumor cells             in BALB/c nude mice were detected by terminal deoxynucleotidyl transferase dUTP             nick-end labeling. Our results show that cisplatin increased the expression of             HERG in gastric cancer cells. Silencing HERG inhibited the apoptosis induced by             cisplatin both in vitro and in vivo, by attenuating the cisplatin effects on Bcl-2,             Bax and active caspase-3. The role of HERG in modulation of cisplatin-induced             apoptosis suggests that HERG may provide a new potential target for cisplatin             chemotherapy in human gastric cancer.
Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, PR China.
Riping
R
Zhang
Pei
P
Tian
Qiang
Q
Chi
Jizhou
J
Wang
Yongwei
Y
Wang
Lingyu
L
Sun
Yang
Y
Liu
Suli
S
Tian
Qifan
Q
Zhang
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034741:RNA, Small Interfering; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics*
D000970:Antineoplastic Agents; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C000606913:KCNH2 protein, human; D034741:RNA, Small Interfering; D002945:Cisplatin