[Determination of astragaloside IV of eight area in Astragali Radix is by HPLC-ELSD internal standard method].
OBJECTIVE
To establish an HPLC-ELSD internal standard method for determination of astragaloside IV in Astragali Radix.


METHODS
With Ginsenoside Rb2 as internal standard, the separation were carried out on an Agilent TC-C18 (4.6 mm x 150 mm, 3.5 microm) column with methanol-water (72: 28) as mobile phase. The flow rate was 1.0 mL x min(-1) and the drift tube temperature of the ELSD was 75 degrees C. The gas pressure was set at 172.4 kPa using the clean and dry compressed air as spray gas.


RESULTS
There was good linearity in the range of 0.5624-5.624 microg of astragaloside IV (r = 0.9999); The average recovery was 98.06% with RSD of 0. 98%.


CONCLUSIONS
The internal standard method is accurate and reproducible, and suitable for quality control of radix astragali.
Hebei Yiling Medicine Institute, Shijiazhuang 050035, China. peicaiyun@126.com
Caiyun
C
Pei
Zongquan
Z
Wang
Jiming
J
Ja
Jian
J
Song
D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D004365:Drugs, Chinese Herbal / Q000032:analysis; D016014:Linear Models; D012015:Reference Standards; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A