Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles.
OBJECTIVE
To assess the presence of viral RNA or DNA sequences in spent culture media used after ovum pickup (OPU) or embryo culture and in liquid nitrogen (LN) used for oocyte or embryo vitrification in patients seropositive for human immunodeficiency virus (HIV), hepatitis C virus (HCV), and hepatitis B virus (HBV) undergoing IVF cycles.


METHODS
Descriptive study.


METHODS
Private university-affiliated IVF center.


METHODS
Twenty-four women who underwent controlled ovarian stimulation for oocyte vitrification or IVF/ET. A total of 6, 11, and 6 patients were seropositive for HIV, HCV, and HBV, respectively, whereas 1 patient showed a coinfection with HCV and HBV. Seven patients presented positive blood viral load (HIV, n = 1; HBV, n = 1; HCV, n = 5). Sixty-three samples were analyzed: follicular fluid, n = 3; spent culture media, n = 33 (20 after OPU and 13 after embryo culture); and LN, n = 27 (14 and 10 after oocyte and embryo vitrification; and 3 LN storage tank samples).


METHODS
Ovum pickup, oocyte and/or embryo culture, and/or vitrification by the Cryotop open device. Reverse transcription-polymerase chain reaction analysis was performed for viral screening.


METHODS
Detection of viral sequences of HIV, HCV, and HVB.


RESULTS
All the samples analyzed tested negative for the detection of viral RNA or DNA sequences.


CONCLUSIONS
We have not detected viral sequences after culture and vitrification of oocytes/embryos from HIV-, HBV-, and HCV-seropositive patients. These findings represent good evidence of the lack of risk of cross-contamination among seropositive patients, even using an open device for vitrification.
IVI-Valencia, Institut Universitari IVI, Valencia, Spain. ana.cobo@ivi.es
Ana
A
Cobo
José
J
Bellver
María José
MJ
de los Santos
José
J
Remohí
D015925:Cryopreservation / Q000592:standards; D003470:Culture Media / Q000592:standards; D004279:DNA, Viral / Q000032:analysis; D046149:Embryo Culture Techniques / Q000592:standards*; D004866:Equipment Contamination / Q000517:prevention & control; D005260:Female; D005307:Fertilization in Vitro / Q000592:standards*; D006678:HIV / Q000235:genetics / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D006509:Hepatitis B / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control; D006801:Humans; D017053:Infection Control / Q000379:methods*; D009584:Nitrogen; D009865:Oocytes / Q000166:cytology / Q000821:virology*; D011247:Pregnancy; D012367:RNA, Viral / Q000032:analysis; D012307:Risk Factors; D014777:Virus Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000517:prevention & control*; D058989:Vitrification
D003470:Culture Media; D004279:DNA, Viral; D012367:RNA, Viral; D009584:Nitrogen