DNA alkylation lesions and their repair in human cells: modification of the comet assay with 3-methyladenine DNA glycosylase (AlkD).
3-methyladenine DNA glycosylase (AlkD) belongs to a new family of DNA glycosylases; it initiates repair of cytotoxic and promutagenic alkylated bases (its main substrates being 3-methyladenine and 7-methylguanine). The modification of the comet assay (single cell gel electrophoresis) using AlkD enzyme thus allows assessment of specific DNA alkylation lesions. The resulting baseless sugars are alkali-labile, and under the conditions of the alkaline comet assay they appear as DNA strand breaks. The alkylating agent methyl methanesulfonate (MMS) was used to induce alkylation lesions and to optimize conditions for the modified comet assay method with AlkD on human lymphoblastoid (TK6) cells. We also studied cellular and in vitro DNA repair of alkylated bases in DNA in TK6 cells after treatment with MMS. Results from cellular repair indicate that 50% of DNA alkylation is repaired in the first 60 min. The in vitro repair assay shows that while AlkD recognises most alkylation lesions after 60 min, a cell extract from TK6 cells recognises most of the MMS-induced DNA adducts already in the first 15 min of incubation, with maximum detection of lesions after 60 min' incubation. Additionally, we tested the in vitro repair capacity of human lymphocyte extracts from 5 individuals and found them to be able to incise DNA alkylations in the same range as AlkD. The modification of the comet assay with AlkD can be useful for in vitro and in vivo genotoxicity studies to detect alkylation damage and repair and also for human biomonitoring and molecular epidemiology studies.
Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynska dolina, 842 15 Bratislava, Slovakia.
Katarína
K
Hašplová
Alexandra
A
Hudecová
Zuzana
Z
Magdolénová
Magnar
M
Bjøras
Eliška
E
Gálová
Eva
E
Miadoková
Mária
M
Dušinská
D000478:Alkylation; D002460:Cell Line; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D018736:DNA Adducts / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D045647:DNA Glycosylases / Q000494:pharmacology*; D004260:DNA Repair; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000201:enzymology; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*; D013997:Time Factors
D018736:DNA Adducts; D009153:Mutagens; D004247:DNA; D008741:Methyl Methanesulfonate; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases