Reduction of perchlorate by salt tolerant bacterial consortia.
Two perchlorate-reducing bacterial consortia (PRBC) were obtained by enrichment cultures from polluted marine sediments. Non-salt-tolerant PRBC (N-PRBC) was enriched without the addition of NaCl, and salt tolerant-PRBC (ST-PRBC) was enriched with 30 g-NaCl L(-1). Although the perchlorate reduction rates decreased with increasing NaCl concentration, ST-PRBC (resp., N-PRBC) could reduce perchlorate until 75 g-NaCl L(-1) (resp., 30 g-NaCl L(-1)). The reduction yield (1.34±0.05 mg-perchlorate per mg-acetate) and maximum perchlorate reduction rate (86 mg-perchlorateL(-1) h(-1)) of ST-PRBC was higher than those (1.16±0.03 mg-perchlorate per mg-acetate and 48 mg-perchlorate L(-1) h(-1)) of N-PRBC. Kinetic analysis showed that NaCl acted as an uncompetitive inhibitor against both PRBCs. The inhibition constants were 25 and 41 mg-NaCl L(-1) for N-PRBC and ST-PRBC, respectively.
Department of Chemical Engineering, Soongsil University, Seoul 156-743, South Korea. hwryu@ssu.ac.kr
Hee Wook
HW
Ryu
Seong Jin
SJ
Nor
Kyung Eun
KE
Moon
Kyung-Suk
KS
Cho
Daniel K
DK
Cha
Kang In
KI
Rhee
D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D010472:Perchlorates / Q000378:metabolism*; D010802:Phylogeny; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D010472:Perchlorates; D012965:Sodium Chloride; C494474:perchlorate