Description and validation of coupling high performance liquid chromatography with resonance Rayleigh scattering in aminoglycosides determination.
In view of the fact that many substances generally exhibit very little ultraviolet absorbance and the absence of native fluorescence, a new strategy with simple instrumentation and excellent analytical performance combining high performance liquid chromatography (HPLC) with resonance Rayleigh scattering (RRS) was developed. It was validated for the quantification of aminoglycosides (AGs). This fact was also carefully calculated by quantum chemistry. However, the sensitivity was probably limited by the volume of flow-through cell. Therefore, the result calls for a suitable one to ensure optimal RRS signal. Interestingly, when serum or urine samples of analytes were analyzed by this method, they were all well resolved without any interference, which would hold a new perspective to be applied in the determination of substances in biological matrix.
Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, China.
Lei
L
Zhang
Jingdong
J
Peng
Jinxia
J
Tang
Binfang
B
Yuan
Rongxing
R
He
Ying
Y
Xiao
D000617:Aminoglycosides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D008027:Light; D008958:Models, Molecular; D008968:Molecular Conformation; D012542:Scattering, Radiation
D000617:Aminoglycosides; D007202:Indicators and Reagents