[Genetic polymorphisms of vascular endothelial growth factor in pre-eclampsia].
OBJECTIVE
To identify genetic polymorphisms of endothelial growth factor (VEGF), positions +936C/T and -2578C/A, in women with pre-eclampsia.


METHODS
This was a cross-sectional study conducted on 80 women divided into two groups: pre-eclampsia and control. The sample was characterized using a pre-structured interview and data transcribed from the medical records. DNA extraction, amplification of sequences by the Polymerase Chain Reaction (PCR) with specific primers and polymorphism analysis of Restriction Fragment Length Polymorphism (RFLP) were performed to identify polymorphisms. The statistical analysis was performed in a descriptive manner and using the x² test. The multiple logistic regression model was used to determine the effect of polymorphisms on pre-eclampsia.


RESULTS
A higher frequency of the T allele of the VEGF +936C/T polymorphism was observed in patients with pre-eclampsia, but with no significant difference. The presence of allele A of the VEGF -2578C/A was significantly higher in the control group.


CONCLUSIONS
No significant association was observed between VEGF +936C/T polymorphism and pre-eclampsia. For the VEGF -2578C/A polymorphism a significant difference was observed between the control and pre-eclampsia group, with allele A being the most frequent in the control, suggesting the possibility that carriers of allele A have lower susceptibility to the development of pre-eclampsia.
Disciplina de Genética,Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brasil.
Valquiria Maria de Paula
VM
Cunha
Roseane Lopes da Silva
RL
Grecco
Marina Carvalho
MC
Paschoini
Sueli Riul da
SR
Silva
Mariângela Torreglosa
MT
Ruiz
Marly Aparecida Spadotto
MA
Balarin
D000328:Adult; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D042461:Vascular Endothelial Growth Factor A