Amphotericin B and fluconazole susceptibility of Candida species determined by cell-chip technology.
The aim of this study was to apply the microfluidic cell-chip technology for susceptibility testing. The cell-chip technology was tested with ATCC Candida strains to determine their viability and susceptibility against amphotericin B and fluconazole. Fungal cells were labelled by Sytox Green, and measurements were carried out in the cell chips of the Agilent Bioanalyzer 2100 system. Results obtained by the chip technology were compared with the standard macrodilution method and conventional flow cytometry. Determination of minimum inhibitory concentration values was based on the differentiation between living and dead cells. The cell-chip method was found to be suitable for the detection of Candida cells, for the differentiation between dead and living cells and for the determination of amphotericin B and fluconazole susceptibility of fungal cells. The minimum inhibitory concentration values obtained by the standard macrodilution, the flow cytometry and the cell-chip method showed good correlation.
Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Pécs, Hungary. bouquet@gamma.ttk.pte.hu
Orsolya
O
Bouquet
Béla
B
Kocsis
Ferenc
F
Kilár
Tamás
T
Lóránd
Ildikó
I
Kustos
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
D000935:Antifungal Agents; D000666:Amphotericin B