Detection of heat shock proteins 70 in the gill, liver, and cardiac muscle of Carassius auratus with confocal microscopy.
Heat shock proteins 70 (Hsp70) are the most extensively studied heat shock proteins for the cellular abundance and cytoprotective effects. Hsp70 induction and subsequent quantification has been used as a sensitive system for aquatic toxicity risk assessment. In this study, the confocal microscopy was used to localize Hsp70 in Carassius auratus (C. auratus) with immunohistochemical technology. There are different zooms to select to analyze the object at the same field of vision with one objective lens with confocal microscopy. It need not change objective lens to observe the details of tissues. In this study, the tissue slices of C. auratus were observed with the 20-fold objective lens. Furthermore, the zooms of 1, 2, and 3 were used to acquire the distribution of Hsp70 in the tissue slices of C. auratus, and the clearer images of Hsp70 in the tissues were acquired. The results indicated that Hsp70 were present in the gill, liver, and cardiac muscle of C. auratus, and a method was established to detect Hsp70 in the tissues of C. auratus with confocal microscopy.
School of Life Sciences, Shandong University of Technology, 255049, Zibo, China. liudongwu@sdut.edu.cn
Dongwu
D
Liu
Zhiwei
Z
Chen
Xuqian
X
Zhou
D000818:Animals; D005880:Gills / Q000737:chemistry*; D006054:Goldfish / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000379:methods*; D009206:Myocardium / Q000737:chemistry*
D018840:HSP70 Heat-Shock Proteins