Simultaneous determination of naringin, hesperidin, neohesperidin, naringenin and hesperetin of Fractus aurantii extract in rat plasma by liquid chromatography tandem mass spectrometry.
A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of naringin, hesperidin, neohesperidin, naringenin and hesperetin in rat plasma, using liquiritin as the internal standard. Plasma samples extracted with a solid-phase extraction procedure were separated on a Zorbax SB-C18 analytical column (2.1 mm × 150 mm, 5 μm) and detected by electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The calibration curves were linear over the range of 3.0-600 ng/ml for naringin, 0.5-100 ng/ml for hesperidin, 3.5-700 ng/ml for neohesperidin, 5.0-1000 ng/ml for naringenin and hesperetin, respectively. The lower limits of quantification were 0.5 ng/ml for naringin, hesperidin, naringenin and hesperetin, and 0.35 ng/ml for neohesperidin. Intra- and inter-day precision (RSD%) was less than 15% and accuracy (RE%) ranged from -3.3% to 4.8%. The validated method was successfully applied to investigate the pharmacokinetics of the major flavanones of Fructus aurantii extract after oral administration to rats.
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
Ling
L
Tong
Dandan
D
Zhou
Jun
J
Gao
Yonghong
Y
Zhu
He
H
Sun
Kaishun
K
Bi
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D044950:Flavanones / Q000097:blood*; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000097:blood*; D006569:Hesperidin / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D044950:Flavanones; D005960:Glucosides; D006569:Hesperidin; C005273:naringenin; C005274:naringin; C013015:hesperetin; C512196:liquiritin