Preclinical and clinical evaluation of intraductally administered agents in early breast cancer.
Most breast cancers originate in the epithelial cells lining the breast ducts. Intraductal administration of cancer therapeutics would lead to high drug exposure to ductal cells and eliminate preinvasive neoplasms while limiting systemic exposure. We performed preclinical studies in N-methyl-N'-nitrosourea-treated rats to compare the effects of 5-fluorouracil, carboplatin, nanoparticle albumin-bound paclitaxel, and methotrexate to the previously reported efficacy of pegylated liposomal doxorubicin (PLD) on treatment of early and established mammary tumors. Protection from tumor growth was observed with all five agents, with extensive epithelial destruction present only in PLD-treated rats. Concurrently, we initiated a clinical trial to establish the feasibility, safety, and maximum tolerated dose of intraductal PLD. In each eligible woman awaiting mastectomy, we visualized one ductal system and administered dextrose or PLD using a dose-escalation schema (2 to 10 mg). Intraductal administration was successful in 15 of 17 women with no serious adverse events. Our preclinical studies suggest that several agents are candidates for intraductal therapy. Our clinical trial supports the feasibility of intraductal administration of agents in the outpatient setting. If successful, administration of agents directly into the ductal system may allow for "breast-sparing mastectomy" in select women.
Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA. vstearn1@jhmi.edu
Vered
V
Stearns
Tsuyoshi
T
Mori
Lisa K
LK
Jacobs
Nagi F
NF
Khouri
Edward
E
Gabrielson
Takahiro
T
Yoshida
Scott L
SL
Kominsky
David L
DL
Huso
Stacie
S
Jeter
Penny
P
Powers
Karineh
K
Tarpinian
Regina J
RJ
Brown
Julie R
JR
Lange
Michelle A
MA
Rudek
Zhe
Z
Zhang
Theodore N
TN
Tsangaris
Saraswati
S
Sukumar
D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy*; D016190:Carboplatin / Q000008:administration & dosage / Q000627:therapeutic use; D004317:Doxorubicin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000627:therapeutic use; D004333:Drug Administration Routes; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage / Q000627:therapeutic use; D006801:Humans; D008321:Mammary Glands, Animal; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy; D008770:Methylnitrosourea / Q000627:therapeutic use; D017239:Paclitaxel / Q000008:administration & dosage / Q000627:therapeutic use; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D055815:Young Adult
D000970:Antineoplastic Agents; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D008770:Methylnitrosourea; D004317:Doxorubicin; D016190:Carboplatin; D017239:Paclitaxel; D005472:Fluorouracil