[Role of carcinoembryonic antigen and cyclooxygenase-2 in the study of molecule incisal edge for colorectal cancer].
OBJECTIVE
To investigate the expression of cyclooxygenase-2(COX-2) and CEA in the tissues adjacent to the tumor within different distances.


METHODS
A total of 42 colorectal cancer tissues were collected.The adjacent tissues within 3 cm to the tumor were procured every 1 cm. Normal tissue was also collected. RNA was extracted and the expression of CEA and COX-2 was detected by RT-PCR.


RESULTS
The CEA mRNA levels of the tumor, the tissues of every 1 cm adjacent to the tumor, and the normal tissue were 135.2 ± 23.3, 78.2 ± 17.3, 75.9 ± 16.5, 56.2 ± 10.7, 52.3 ± 12.8, 18.2 ± 7.9, 16.2 ± 6.5, and 16.6 ± 7.0. The levels of COX-2 mRNA in above positions were 134.9 ± 31.1, 79.2 ± 20.2, 77.0 ± 20.5, 62.7 ± 21.9, 58.0 ± 18.1, 21.2 ± 10.3, 18.3 ± 7.6, and 17.1 ± 6.3. These data showed a decreasing trend of CEA and COX-2 as the distance increased from the tumor. The CEA mRNA levels showed positive correlation with the levels of COX-2 mRNA(r=0.725, P<0.01).


CONCLUSIONS
CEA and COX-2 may be considered to be used as biomarkers for the study of molecular resection margin of colorectal cancer.
Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou, China.
Xiao-dong
XD
Yang
Chun-gen
CG
Xing
Zhi-dong
ZD
Zhao
Wei
W
Gong
Yong-you
YY
Wu
Feng-yun
FY
Zhong
Xiao-dong
XD
Lv
Kui
K
Zhao
D000328:Adult; D000368:Aged; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
D002272:Carcinoembryonic Antigen; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human