High prevalence and lack of diversity of Wolbachia pipientis in Aedes albopictus populations from Northeast Brazil.
The use of Wolbachia as a tool to control insect vectors has recently been suggested. In this context, studies on the prevalence and diversity of this bacterium in wild populations are relevant. Here, we evaluated the diversity of two Wolbachiagenes (ftsZ and wsp) and the prevalence of this endosymbiont in wild Aedes albopictus. Using semi-nested polymerase chain reaction, our results showed that 99.3% of the individuals were superinfected with Wolbachia. In regards to genetic diversity, the two genes showed no variation within or among mosquito populations. An analysis of other Wolbachia markers may help to clarify the relationship between insect and endosymbiont.
Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, PE, Brasil.
Alessandra Lima
AL
de Albuquerque
Tereza
T
Magalhães
Constância Flávia Junqueira
CF
Ayres
D000330:Aedes / Q000382:microbiology*; D000818:Animals; D014644:Genetic Variation / Q000235:genetics; D007303:Insect Vectors / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D020577:Wolbachia / Q000235:genetics* / Q000302:isolation & purification