The effects of dan-shen root on cardiomyogenic differentiation of human placenta-derived mesenchymal stem cells.
The aim of this study was to search for a good inducer agent using for cardiomyogenic differentiation of stem cells. Human placenta-derived mesenchymal stem cells (hPDMSCs) were isolated and incubated in enriched medium. Fourth passaged cells were treated with 10mg/L dan-shen root for 20 days. Morphologic characteristics were analyzed by confocal and electron microscopy. Expression of α-sarcomeric actin was analyzed by immunohistochemistry. Expression of cardiac troponin-I (TnI) was analyzed by immunohistofluorescence. Atrial natriuretic factor (ANF) and beta-myocin heavy chain (β-MHC) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). hPDMSCs treated with dan-shen root gradually formed a stick-like morphology and connected with adjoining cells. On the 20th day, most of the induced cells stained positive with α-sarcomeric actin and TnI antibody. ANF and β-MHC were also detected in the induced cells. Approximately 80% of the cells were successfully transdifferentiated into cardiomyocytes. In conclusion, dan-shen root is a good inducer agent used for cardiomyogenic differentiation of hPDMSCs.
Department of Clinical Laboratory, The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China.
Kun
K
Li
Shi-zheng
SZ
Li
Yun-li
YL
Zhang
Xue-zhe
XZ
Wang
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics; D024722:Cardiac Myosins; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D024510:Muscle Development / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology*; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D027561:Salvia miltiorrhiza
C501641:MYH7 protein, human; D009320:Atrial Natriuretic Factor; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains