Quantitative proteomics reveals new insights into erythrocyte invasion by Plasmodium falciparum.
Differential expression of ligands in the human malaria parasite Plasmodium falciparum enables it to recognize different receptors on the erythrocyte surface, thereby providing alternative invasion pathways. Switching of invasion from using sialated to nonsialated erythrocyte receptors has been linked to the transcriptional activation of a single parasite ligand. We have used quantitative proteomics to show that in addition to this single known change, there are a significant number of changes in the expression of merozoite proteins that are regulated independent of transcription during invasion pathway switching. These results demonstrate a so far unrecognized mechanism by which the malaria parasite is able to adapt to variations in the host cell environment by post-transcriptional regulation.
School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
Claudia
C
Kuss
Chee Sian
CS
Gan
Karthigayan
K
Gunalan
Zbynek
Z
Bozdech
Siu Kwan
SK
Sze
Peter Rainer
PR
Preiser
D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D020869:Gene Expression Profiling; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016778:Malaria, Falciparum / Q000235:genetics / Q000378:metabolism / Q000469:parasitology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000378:metabolism; D052940:Merozoites / Q000378:metabolism* / Q000469:parasitology*; D020411:Oligonucleotide Array Sequence Analysis; D010446:Peptide Fragments / Q000032:analysis; D010963:Plasmodium falciparum / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D040901:Proteomics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D020066:Merozoite Surface Protein 1; D010446:Peptide Fragments; D015800:Protozoan Proteins; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase