LC-MS determination of oxidized and reduced glutathione in human dermis: a microdialysis study.
A simple, highly selective, sensitive and reproducible liquid chromatography-electrospray ionization mass spectrometry method has been developed for the direct and simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in microdialysis samples from human dermis. Chromatographic separation was carried out on an MODULO CART QS KROMASIL 5C18 (250 mm × 2 mm × 5 μm) analytical column at a flow rate of 0.25 ml/min. An isocratic mode was used and consisted of acidified water and acetonitrile (50/50, v/v). To improve the sensitivity, silver nitrate was added as post-column reagent. A trap mass spectrum was used equipped with an ESI interface. The limits of detection and quantification were respectively 0.12 and 0.4 ng/ml for GSH and 0.2 and 0.5 ng/ml for GSSG. Intra-day and inter-day accuracy and precision were determined and the variability was less than 6.2% (R.S.D.).
SARL BIOEXIGENCE, Besançon, France.
Sophie
S
Robin
Nathalie
N
Leveque
Carol
C
Courderot-Masuyer
Philippe
P
Humbert
D002853:Chromatography, Liquid / Q000379:methods*; D020405:Dermis / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D019803:Glutathione Disulfide / Q000032:analysis*; D006801:Humans; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods; D017551:Microdialysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012835:Silver Nitrate
D012835:Silver Nitrate; D005978:Glutathione; D019803:Glutathione Disulfide