Persistent Chlamydia trachomatis infection of HeLa cells mediates apoptosis resistance through a Chlamydia protease-like activity factor-independent mechanism and induces high mobility group box 1 release.
Intracellular persistence of Chlamydia trachomatis has been implicated in the development of chronic infection that can result in pelvic inflammatory disease and tubal sterility. By inhibition of host cell apoptosis, chlamydiae have evolved a strategy to maintain the intracellular environment for replication and persistence. Both antiapoptotic host cell-derived factors and the chlamydial protease-like activity factor (CPAF) are involved in Chlamydia-mediated apoptosis resistance. Here, we show that in HeLa cells infected with gamma interferon (IFN-γ)-induced persistent C. trachomatis serovar D, the expression of CPAF is downregulated, and proapoptotic protease substrates are not cleaved. Persistent infection protected HeLa cells from apoptosis when they were exposed to staurosporine. Small-interfering RNA-mediated inhibition of myeloid cell leukemia 1 (Mcl-1) protein upregulation sensitized persistently infected cells for apoptosis. The inhibitor of apoptosis protein 2 (IAP-2) seems not to be relevant in this context because IAP-2 protein was not induced in response to IFN-γ treatment. Although apoptosis was inhibited, persistent infection caused cell membrane disintegration, as measured by the increased release of cytokeratin 18 from HeLa cells. Moreover, persistently infected cells released significantly increased amounts of high mobility group box 1 (HMGB1) protein which represents a proinflammatory damage-associated pattern molecule. The data of this study suggest that cells infected with persistent C. trachomatis are protected from apoptosis independently of CPAF but may promote chronic inflammation through HMGB1 release.
Institute of Medical Microbiology, Clinic for Anesthesiology and Intensive Care Medicine, Friedrich Schiller University of Jena, Jena, Germany. juergen.roedel@med.uni-jena.de
Jürgen
J
Rödel
Christina
C
Grosse
Hangxing
H
Yu
Katharina
K
Wolf
Gordon P
GP
Otto
Elisabeth
E
Liebler-Tenorio
Vera
V
Forsbach-Birk
Eberhard
E
Straube
D017209:Apoptosis; D002462:Cell Membrane / Q000502:physiology; D002470:Cell Survival; D002692:Chlamydia trachomatis / Q000201:enzymology / Q000472:pathogenicity*; D010450:Endopeptidases / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D024243:HMGB1 Protein / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D053538:Keratin-18 / Q000378:metabolism; D019311:Staurosporine / Q000633:toxicity; D037521:Virulence Factors / Q000378:metabolism*
D024243:HMGB1 Protein; D053538:Keratin-18; D037521:Virulence Factors; D007371:Interferon-gamma; D010450:Endopeptidases; C425923:CPA factor; D019311:Staurosporine