Mediterranean diet supplemented with coenzyme Q10 modifies the expression of proinflammatory and endoplasmic reticulum stress-related genes in elderly men and women.
We have investigated whether the quality of dietary fat and supplementation with coenzyme Q(10) (CoQ) modifies expression of genes related with inflammatory response and endoplasmic reticulum stress in elderly persons. Twenty participants received three diets for 4 weeks each: Mediterranean diet + CoQ (Med + CoQ), Mediterranean diet (Med), and saturated fatty acid-rich diet (SFA). After 12-hour fast, volunteers consumed a breakfast with a fat composition similar to that consumed in each of the diets. Med and Med + CoQ diets produced a lower fasting calreticulin, IL-1b, and JNK-1 gene expression; a lower postprandial p65, IKK-b, MMP-9, IL-1b, JNK-1, sXBP-1, and BiP/Grp78 gene expression; and a higher postprandial IkB-a gene expression compared with the SFA diet. Med + CoQ diet produced a lower postprandial decrease p65 and IKK-b gene expression compared with the other diets. Our results support the anti-inflammatory effect of Med diet and that exogenous CoQ supplementation in synergy with a Med diet modulates the inflammatory response and endoplasmic reticulum stress.
Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital, University of Cordoba, Córdoba, Spain.
Elena M
EM
Yubero-Serrano
Lorena
L
Gonzalez-Guardia
Oriol
O
Rangel-Zuñiga
Javier
J
Delgado-Lista
Francisco M
FM
Gutierrez-Mariscal
Pablo
P
Perez-Martinez
Nieves
N
Delgado-Casado
Cristina
C
Cruz-Teno
Francisco J
FJ
Tinahones
Jose M
JM
Villalba
Francisco
F
Perez-Jimenez
Jose
J
Lopez-Miranda
D000368:Aged; D015992:Body Mass Index; D037282:Calreticulin / Q000096:biosynthesis / Q000235:genetics; D018592:Cross-Over Studies; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D038441:Diet, Mediterranean; D004041:Dietary Fats / Q000378:metabolism; D019587:Dietary Supplements; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D051550:I-kappa B Kinase / Q000096:biosynthesis / Q000235:genetics; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D048055:Mitogen-Activated Protein Kinase 8 / Q000096:biosynthesis / Q000235:genetics; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D037282:Calreticulin; D004268:DNA-Binding Proteins; D004041:Dietary Fats; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D053583:Interleukin-1beta; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D014451:Ubiquinone; D051550:I-kappa B Kinase; D048055:Mitogen-Activated Protein Kinase 8; D020780:Matrix Metalloproteinase 9; C024989:coenzyme Q10