Protein-triggered instant disassembly of biomimetic Layer-by-Layer films.
Layer-by-Layer (LbL) coatings are promising tools for the biofunctionalization of biomaterials, as they allow stress-free immobilization of proteins. Here, we explore the possibility to immobilize phosvitin, a highly phosphorylated protein viewed as a model of bone phosphoproteins and, as such, a potential promotive agent of surface-directed biomineralization, into biomimetic LbL architectures. Two immobilization protocols are attempted, first, using phosvitin as the polyanionic component of phosvitin/poly-(L-lysine) films and, second, adsorbing it onto preformed chondroitin sulfate/poly-(L-lysine) films. Surprisingly, it is neither possible to embed phosvitin as the constitutive polyanion of the LbL architectures nor to adsorb it atop preformed films. Instead, phosvitin triggers instant massive film disassembly. This unexpected, incidentally detected behavior constitutes the first example of destructive interactions between LbL films and a third polyelectrolyte, a fortiori a protein, which might open a route toward new stimuli-responsive films for biosensing or drug delivery applications. Interestingly, additional preliminary results still indicate a promotive effect of phosvitin-containing remnant films on calcium phosphate deposition.
Laboratoire de Biophysique et Biomatériaux (La2B), SMS EA 3233, IMR FED 4114, Université de Rouen, Centre Universitaire d'Evreux, 1 rue du 7ème Chasseurs, BP 281, 27002 Evreux Cedex, France.
Khalil
K
Abdelkebir
Fabien
F
Gaudière
Sandrine
S
Morin-Grognet
Gérard
G
Coquerel
Hassan
H
Atmani
Béatrice
B
Labat
Guy
G
Ladam
D001672:Biocompatible Materials / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry; D008567:Membranes, Artificial; D010774:Phosvitin / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011107:Polylysine; D002809:Chondroitin Sulfates; D010774:Phosvitin