Production of succinic acid from sucrose and sugarcane molasses by metabolically engineered Escherichia coli.
Sucrose-utilizing genes (cscKB and cscA) from Escherichia coli KO11 were cloned and expressed in a metabolically engineered E. coli KJ122 to enhance succinate production from sucrose. KJ122 harboring a recombinant plasmid, pKJSUC, was screened for the efficient sucrose utilization by growth-based selection and adaptation. KJ122-pKJSUC-24T efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. Succinate concentrations of 51 g/L (productivity equal to 1.05 g/L/h) were produced from sucrose in anaerobic bottles, and concentrations of 47 g/L were produced in 10L bioreactor within 48 h. Antibiotics had no effect on the succinate production by KJ122-pKJSUC-24T. In addition, succinate concentrations of 62 g/L were produced from sugarcane molasses in anaerobic bottles, and concentrations of 56 g/L in 10 L bioreactor within 72 h. These results demonstrated that KJ122-pKJSUC-24T would be a potential strain for bio-based succinate production from sucrose and sugarcane molasses.
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Ave., Suranaree, Muang, Nakhon Ratchasima 30000, Thailand.
Sitha
S
Chan
Sunthorn
S
Kanchanatawee
Kaemwich
K
Jantama
D000693:Anaerobiosis / Q000187:drug effects; D055162:Biocatalysis / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D008965:Molasses; D010957:Plasmids / Q000235:genetics; D031786:Saccharum / Q000737:chemistry*; D019802:Succinic Acid / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism* / Q000494:pharmacology
D003470:Culture Media; D005632:Fructose; D013395:Sucrose; D019802:Succinic Acid; D005947:Glucose