Characterization of Epstein-Barr virus gp350/220 gene variants in virus isolates from gastric carcinoma and nasopharyngeal carcinoma.
To characterize the sequence variation of the gp350/220 and explore its potential association with EBV-associated tumors, the gp350/220 gene was sequenced from 41 EBV-associated gastric carcinoma (EBVaGC) and 81 nasopharyngeal carcinoma (NPC) biopsies as well as 35 throat washing (TW) samples from healthy donors. Preferential linkages between variants of the N-terminus of gp350/220 and EBNA3C variants were detected, and type A/BLLF1-a was the dominant variant in this study. The dominant variant in the C-terminal region of gp350/220 was 9P. The similar distribution of gp350/220 variants in NPC, EBVaGC and healthy donors suggest that gp350/220 variations are geographically restricted rather than tumor-specific polymorphisms.
Department of Medical Microbiology, Qingdao University Medical College, Qingdao 266021, China. qdluobing@yahoo.com
Bing
B
Luo
Mengyang
M
Liu
Yan
Y
Chao
Yun
Y
Wang
Yongzheng
Y
Jing
Zhifu
Z
Sun
D000595:Amino Acid Sequence; D002277:Carcinoma / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000821:virology*; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D010802:Phylogeny; D016415:Sequence Alignment; D013274:Stomach Neoplasms / Q000821:virology*; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins