Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor.
NKp46 is a cell surface receptor expressed on natural killer (NK) cells, on a minute subset of T cells, and on a population of innate lymphoid cells that produce IL-22 and express the transcription factor retinoid-related orphan receptor (ROR)-γt, referred to as NK cell receptor (NKR)(+)ROR-γt(+) cells. Here we describe Nkp46(iCre) knock-in mice in which the gene encoding the improved Cre (iCre) recombinase was inserted into the Nkp46 locus. This mouse was used to noninvasively trace cells expressing NKp46 in vivo. Fate mapping experiments demonstrated the stable expression of NKp46 on NK cells and allowed a reappraisal of the sequential steps of NK cell maturation. NKp46 genetic tracing also showed that gut NKR(+)ROR-γt(+) and NK cells represent two distinct lineages. In addition, the genetic heterogeneity of liver NK cells was evidenced. Finally, Nkp46(iCre) mice also represent a unique mouse model of conditional mutagenesis specifically in NKp46(+) cells, paving the way for further developments in the biology of NKp46(+) NK, T, and NKR(+)ROR-γt(+) cells.
Centre d'Immunologie de Marseille-Luminy,  Université  de la Méditerranée UM 631, Campus de Luminy Case 906, 13288 Marseille,  France.
Emilie
E
Narni-Mancinelli
Julie
J
Chaix
Aurore
A
Fenis
Yann M
YM
Kerdiles
Nadia
N
Yessaad
Ana
A
Reynders
Claude
C
Gregoire
Herve
H
Luche
Sophie
S
Ugolini
Elena
E
Tomasello
Thierry
T
Walzer
Eric
E
Vivier
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D007422:Intestines / Q000166:cytology; D008099:Liver / Q000166:cytology; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D000950:Antigens, Ly; D055627:Natural Cytotoxicity Triggering Receptor 1; C118384:Ncr1 protein, mouse