Isolation of three antibacterial naphthoquinones from Plumbago indica roots and development of a validated quantitative HPLC analytical method.
Three naphthoquinones, plumbagin (1), 3,3'-biplumbagin (2) and elliptinone (3), isolated from Plumbago indica roots by antibacterial bioassay-guided isolation, were used as standard markers for quantitative determination. A reversed-phase HPLC method was established for the simultaneous determination of the naphthoquinones in P. indica root extracts. The method utilised a Phenomenex® ODS column (4.6 × 150 mm, 5 µm) at 25°C with a mixture of methanol and 5% aqueous acetic acid (80 : 20 v/v) as the mobile phase at a flow rate of 0.85 mL/min, and UV detection at 260 nm. The parameters of linearity, precision, accuracy specificity and sensitivity of the method were evaluated. The recovery of the method was 98.6-100.6% with good linearity (r (2 )≥ 0.9997) for all three naphthoquinones. A high degree of sensitivity, specificity as well as repeatability and reproducibility (R.S.D. values less than 5%) were also achieved.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.
Sermwut
S
Kaewbumrung
Pharkphoom
P
Panichayupakaranant
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D009285:Naphthoquinones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D029606:Plumbaginaceae / Q000737:chemistry*; D011425:Propionibacterium acnes / Q000187:drug effects; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D009285:Naphthoquinones; D010936:Plant Extracts; C000590679:elliptinone; C079946:3,3'-biplumbagin; C014758:plumbagin