Capillary zone electrophoresis as a potential technique for the simultaneous determination of sulfadoxine and pyrimethamine in tablet formulations.
A novel, simple and rapid capillary zone electrophoresis method with UV detection has been developed for the simultaneous determination of pyrimethamine and sulfadoxine in tablet formulations. The compounds are separated in 6 min in a fused silica capillary, 30 cm long (20 cm to detector)× 50 μm using a 100 mM phosphate buffer pH 7.2 as background electrolyte, a 330 V cm(-1) electric field and a detection wavelength of 214 nm. Analysis of different tablet formulations has shown a good agreement with the liquid chromatography method described in the United States Pharmacopoeia. Main advantages of the CZE method are the rapid set-up of instrumentation and capillary equilibration, short analysis time and low running cost.
Laboratoire de Chimie Analytique, Contrôle physico-chimique des médicaments, Institut des Biomolécules Max Mousseron, UMR 5247, Faculté de Pharmacie, BP 14491, 34093 Montpelier, France.
N'Cho Christophe
NC
Amin
Marie-Dominique
MD
Blanchin
Michèle
M
Aké
Huguette
H
Fabre
D002021:Buffers; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010710:Phosphates / Q000737:chemistry; D011739:Pyrimethamine / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013413:Sulfadoxine / Q000032:analysis* / Q000737:chemistry; D013607:Tablets / Q000032:analysis / Q000737:chemistry
D002021:Buffers; D004573:Electrolytes; D010710:Phosphates; D013607:Tablets; D013413:Sulfadoxine; D011739:Pyrimethamine