Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM.
Optimization of three parameters, temperature (25-35 degrees C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-15% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31 C) temperature and 5.5% (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL-1 were recorded to be closed to the predicted value (44.80 IU mL-1). Under the optimized SSF condition (31 degrees C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IU mL-1 compare to before optimized (30 degrees C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL-1.
School of Bioscience and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600, Bangi, Malaysia.
Jahwarhar Izuan Abdul
JI
Rashid
Noraini
N
Samat
Wan
W
Mohtar
Wan
W
Yusoff
D001230:Aspergillus / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D006813:Humidity; D007218:Industrial Microbiology / Q000379:methods*; D013696:Temperature; D044902:beta-Mannosidase / Q000096:biosynthesis*
D044902:beta-Mannosidase