Neural cell type-specific responses to glycomimetic functionalized collagen.
Despite their noted functional role, glycans have had limited therapeutic use due to difficulties in synthesis and quick degradation in vivo. The recent discovery of glycomimetics has provided new opportunities for their application. In this study, we have functionalized type I collagen with peptide mimics of two glycans: (1) polysialic acid (PSA) and (2) an epitope first discovered on human natural killer cells (HNK-1). These glycans and their glycomimetic counterparts have been shown to be important regulators of repair following injury through their unique and phenotypically specific effects on neural behavior. We show that these molecules retain their bioactivity following functionalization to the collagen backbone. Grafted HNK-1 encouraged motor neuron outgrowth, while grafted PSA encouraged sensory and motor neuron outgrowth and enhanced Schwann cell proliferation and process extension. These data support the potential of glycomimetic-functionalized collagen as a biomaterial strategy to increase the efficiency of synaptic reconnection following nervous system injury.
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Shirley N
SN
Masand
Isaac J
IJ
Perron
Melitta
M
Schachner
David I
DI
Shreiber
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D012794:Sialic Acids / Q000737:chemistry; D013116:Spinal Cord / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods
D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; D003094:Collagen