Characterization of novel canine bocaviruses and their association with respiratory disease.
We report the first identification, genetic characterization and disease association studies of several novel species of canine bocaviruses (CBoV). Evolutionary analysis confirmed that CBoV are genetically distinct from the only other known canine bocavirus, minute virus of canines, with which they share less than 63, 62 and 64 % protein identity in NS, NP and VP genes, respectively. Comparative genetic analysis of 37 VP gene variants found in diseased and healthy animals showed that these novel viruses are genetically highly diverse and are common in canine respiratory infections that have remained undetected until now. Interestingly, we observed that a CBoV genotype with a unique deletion in the VP2 gene was significantly more prevalent in animals with respiratory diseases compared with healthy animals.
Center for Infection and Immunity, Columbia University, New York 10032, USA.
Amit
A
Kapoor
Center for Infection and Immunity, Columbia University, New York 10032, USA.
Natasha
N
Mehta
College of Veterinary Medicine at Cornell, Ithaca, NY 14853, USA.
Edward J
EJ
Dubovi
Centre for Immunology, Infection and Evolution, University of Edinburgh, Edinburgh EH9 3JT, UK.
Peter
P
Simmonds
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA.
Lakshmanan
L
Govindasamy
Center for Infection and Immunity, Columbia University, New York 10032, USA.
Jan L
JL
Medina
Center for Infection and Immunity, Columbia University, New York 10032, USA.
Craig
C
Street
Pfizer Veterinary Medicine Research and Development, New York 10017, USA.
Shelly
S
Shields
Center for Infection and Immunity, Columbia University, New York 10032, USA.
W Ian
WI
Lipkin
D000818:Animals; D052660:Bocavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins