Identification of complex  vertebral malformation carriers in Holstein cattle in south China.
Complex  vertebral malformation (CVM) is a recently described monogenic autosomal  recessive hereditary defect of Holstein dairy cattle that causes premature  birth, aborted fetuses and stillborn calves. Guanine is substituted by thymine  (G>T) in the solute carrier family 35 member A3 gene (SLC35A3). A valine is  changed to a phenylalanine at position 180 of uridine 5'-diphosphate-N-acetyl-glucosamine  transporter protein. CVM is expected to occur in many countries due to the  widespread use of sire semen. We developed a created restriction site PCR  (CRS-PCR) method to diagnose CVM in dairy cows. This was tested on 217 cows and  125 bulls selected randomly from a Holstein cattle population in south China.  Five Holstein cows and five Holstein bulls were identified to be CVM carriers;  the percentages of CVM carriers were estimated to be 2.3, 4.0 and 2.9% in the  cows, bulls and entire Holstein cattle sample, respectively.
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, Huazhong Agricultural University, Wuhan, China.
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Wang
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Tong
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Hu
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Yang
X Q
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Zhong
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Yu
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Wu
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Liu
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Li
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Hua
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Zhao
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Zhang
D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle / Q000002:abnormalities / Q000235:genetics; D002418:Cattle Diseases / Q000235:genetics*; D002681:China; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D030342:Genetic Diseases, Inborn / Q000235:genetics / Q000662:veterinary; D006579:Heterozygote; D008297:Male; D020125:Mutation, Missense; D033704:Nucleotide Transport Proteins / Q000235:genetics*; D013131:Spine / Q000002:abnormalities*
D033704:Nucleotide Transport Proteins