Structural characterization of the O-chain polysaccharide from an environmentally beneficial bacterium Pseudomonas chlororaphis subsp. aureofaciens strain M71.
Pseudomonas chlororaphis subsp. aureofaciens strain M71 was isolated from the root of a tomato plant and it was able to control in vivo Fusarium oxysporum f. sp. radicis-lycopersici responsible for the tomato crown and root rot. Recently, strain M71 was evaluated even for its efficacy in controlling Seiridium cardinale, the causal agent of bark canker of common cypress (Cupressus sempervirens L.). Strain M71 ability to persist on the tomato rhizosphere and on the aerial part of cypress plants could be related to the nature of the lipopolysaccharides (LPS) present on the outer membrane and in particular to the O-specific polysaccharide. A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide from P. chlororaphis subsp. aureofaciens strain M71. By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as the following linear trisaccharide.
Dipartimento di Chimica Organica e Biochimica, Complesso Universitario di Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy.
Giuseppina
G
Pieretti
Gerardo
G
Puopolo
Sara
S
Carillo
Astolfo
A
Zoina
Rosa
R
Lanzetta
Michelangelo
M
Parrilli
Antonio
A
Evidente
Maria Michela
MM
Corsaro
D000898:Antibiosis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D011549:Pseudomonas
D019081:O Antigens