Autophagosome biogenesis requires SNAREs.
We recently showed that phagophore biogenesis requires SNAREs. Our data indicate that the exocytic Q/t-SNAREs Sso1/2 and Sec9 are required for one of the earliest steps in autophagosome biogenesis, the homotypic fusion of Atg9-containing vesicles. We propose that this step precedes the formation of Atg9-containing tubulovesicular clusters (TVCs) that is a key step in perivacuolar, phagophore assembly. We also found that the endosomal Q/t-SNARE Tlg2 and the R/v-SNAREs Sec22 and Ykt6 interact with Sso1-Sec9, and are required for normal Atg9 trafficking. Thus, autophagosome biogenesis appears to involve multiple SNARE-mediated fusion events. These findings provide novel insights into the mechanism of autophagosome construction.
Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Usha
U
Nair
Daniel J
DJ
Klionsky
D001343:Autophagy; D005089:Exocytosis; D008954:Models, Biological; D010588:Phagosomes / Q000378:metabolism*; D050600:SNARE Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins