Repair of articular cartilage defects with a novel injectable in situ forming material in a canine model.
We developed an ultra-purified in situ forming gel as an injectable delivery vehicle of bone marrow stromal cells (BMSCs). Our objective was to assess reparative tissues treated with autologous BMSCs implanted using the injectable implantation system into osteochondral defects in a canine model. Forty-eight osteochondral defects in the patella groove of the knee joint were created in 12 adult beagle dogs (two defects in each knee). The defects were divided into a defect group (n = 16), an acellular novel material implantation (material) group (n = 16), and a BMSCs implantation using the current vehicle system (material with BMSCs) group (n = 16). The reparative tissues at 16 weeks postoperatively were assessed through gross, histological, and mechanical analyses. The reparative tissues of the material with BMSCs group were substituted with firm and smooth hyaline-like cartilage tissue that was perfectly integrated into the host tissues. This treatment group obviously enhanced the subchondral bone reconstruction. The compressive modulus of the reparative tissues was significantly higher in the material with BMSCs group than the other groups. This study demonstrated that the implantation of BMSCs using our novel in situ forming material induced a mature hyaline-like cartilage repair of osteochondral defects in a canine model.
Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita 15, Nishi 7, Sapporo 060-8638, Japan.
Tatsuya
T
Igarashi
Norimasa
N
Iwasaki
Daisuke
D
Kawamura
Yasuhiko
Y
Kasahara
Yukinori
Y
Tsukuda
Nobuo
N
Ohzawa
Masayuki
M
Ito
Yasuharu
Y
Izumisawa
Akio
A
Minami
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology*; D004285:Dogs; D005260:Female; D007267:Injections; D055595:Mechanical Phenomena / Q000187:drug effects; D023421:Models, Animal; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D001672:Biocompatible Materials