Microwave-mediated enzyme-linked immunosorbent assay procedure.
Here we demonstrate a novel microwave-mediated enzyme-linked immunosorbent assay (MELISA) method that has dramatically reduced the enzyme-linked immunosorbent assay (ELISA) timing to less than 5 min with a result comparable to that obtained by 18-h conventional ELISA. Efficacy of the MELISA procedure is demonstrated by detecting human immunoglobulin G (IgG), rabbit IgG, human immunoglobulin E (IgE), human interleuken 1β (IL-1β), Entamoeba histolytica antibody, and Aspergillus fumigatus antibody. MELISA could be an excellent substitute for time-consuming conventional ELISA for rapid diagnosis of diseases in cases of medical urgency, outbreak of infectious diseases, and screening of samples in blood banks or emigration counters.
Institute of Genomics and Integrative Biology (CSIR), Delhi 110007, India. pnahar@igib.res.in
Pradip
P
Nahar
Utpal
U
Bora
Gainda L
GL
Sharma
Dileep Kumar
DK
Kannoujia
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008872:Microwaves; D011817:Rabbits
D007136:Immunoglobulins