The effect of IL-1 receptor antagonist on orthodontic tooth movement in mice.
OBJECTIVE
Orthodontic tooth movement (OTM) is achieved by alveolar bone remodelling induced by mechanical loading. Whilst interleukin-1 (IL-1) is directly involved in OTM, the role of interleukin-1 receptor antagonist (IL-1Ra), a naturally occurring IL-1 antagonist, is not completely defined. Therefore, the aim of this study was to investigate the effects of IL-1Ra on OTM.


METHODS
An orthodontic appliance was placed in C57BL6 mice treated with vehicle or IL-1Ra (10 mg/kg/day). OTM and TRAP-positive osteoclasts were evaluated after 12 days of mechanical loading and the levels of cytokines on periodontal tissues were analysed by ELISA after 12 and 72 h.


RESULTS
Mice treated with IL-1Ra showed diminished OTM and decreased numbers of TRAP-positive osteoclasts. In line with this, lower levels of IL-1β and TNF-α, and higher levels of IL-10, were observed on periodontal tissues of IL-1Ra-treated mice in relation to the vehicle-treated group.


CONCLUSIONS
The present study suggests that IL-1Ra downregulates OTM, probably by its anti-inflammatory actions.
Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
Juliana Tito
JT
Salla
Silvana Rodrigues de Albuquerque
SR
Taddei
Celso Martins
CM
Queiroz-Junior
Ildeu
I
Andrade Junior
Mauro Martins
MM
Teixeira
Tarcília Aparecida
TA
Silva
D000704:Analysis of Variance; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009967:Orthodontic Appliances; D011897:Random Allocation; D014087:Tooth Movement Techniques
D016207:Cytokines; D053590:Interleukin 1 Receptor Antagonist Protein