Accumulation and activation of natural killer cells in local intraperitoneal HIV-1/MuLV infection results in early control of virus infected cells.
Natural killer (NK) cells are important effectors in resistance to viral infections. The role of NK cells in the acute response to human immunodeficiency virus 1 (HIV-1) infected cells was investigated in a mouse model based on a HIV-1/murine leukemia virus (MuLV) pseudovirus. Splenocytes infected with HIV-1/MuLV were injected intraperitoneally and local immunologic responses and persistence of infected cells were investigated. In vivo depletion with an anti-NK1.1 antibody showed that NK cells are important in resistance to virus infected cells. Moreover, NK cell frequency in the peritoneal cavity increased in response to infected cells and these NK cells had a more mature phenotype, as determined by CD27 and Mac-1 expression. Interestingly, after injection of HIV-1/MuLV infected cells, but not MuLV infected cells, peritoneal NK cells had an increased cytotoxic activity. In conclusion, NK cells play a role in the early control of HIV-1/MuLV infected cells in vivo.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. maria.johansson@ki.se
Susanne E
SE
Johansson
Hanna
H
Brauner
Jorma
J
Hinkula
Britta
B
Wahren
Louise
L
Berg
Maria H
MH
Johansson
D000818:Animals; D057134:Antibodies, Neutralizing / Q000009:adverse effects / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D007274:Injections, Intraperitoneal; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000821:virology; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D016177:Macrophage-1 Antigen / Q000032:analysis / Q000096:biosynthesis; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000821:virology; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000821:virology; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis / Q000096:biosynthesis; D019562:Viral Load / Q000187:drug effects / Q000276:immunology
D057134:Antibodies, Neutralizing; D016177:Macrophage-1 Antigen; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7