Molecular cloning and tissue-specific expression of Toll-like receptor 5 gene from turkeys.
Toll-like receptors (TLRs), a family of transmembrane and cytosolic proteins, detect microbial patterns, initiating innate immune responses in various organisms. Although they are abundant, genetic characterization and functional differences of TLRs in economically important avian species such as chickens and turkeys have not been investigated in detail. In this study, the putative TLR5 coding region from turkey genome was sequenced, and its homology to other vertebrate species was analyzed. Secondary structure analysis revealed protein motifs typical of the chicken TLR5 protein structure, with 97% amino acid identity between them. mRNA expression profiling in adult turkeys revealed abundant TLR5 expression in a broad range of tissues. Stimulation with the TLR5 ligand flagellin resulted in the production of the inflammatory mediators interleukin (IL)-1beta, IL-6, and nitric oxide in peripheral blood mononuclear cells. To our knowledge, this is the first complete turkey TLR5 coding DNA sequence reported in sequence databases.
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, India.
V P
VP
Gopinath
Moanaro
M
Biswas
Gopal Dhinakar
GD
Raj
A
A
Raja
A K
AK
Kumanan
Subbiah
S
Elankumaran
D020816:Amino Acid Motifs; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005408:Flagellin / Q000378:metabolism; D020869:Gene Expression Profiling; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012486:Salmonella typhimurium / Q000276:immunology; D051198:Toll-Like Receptor 5 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014422:Turkeys / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D030161:Avian Proteins; D018076:DNA, Complementary; D053583:Interleukin-1beta; D015850:Interleukin-6; D012333:RNA, Messenger; D051198:Toll-Like Receptor 5; D005408:Flagellin; D009569:Nitric Oxide