Lysophosphatidylcholine induces Ca(2+) mobilization in Jurkat human T lymphocytes and CTLL-2 mouse T lymphocytes by different pathways.
Lysophosphatidylcholine (LPC), an important compound in the immune system, regulates a variety of biological processes. We examined and compared the effect of exogenous LPC on intracellular Ca(2+) overload in human Jurkat CD4+ T lymphocytes and mouse CTLL-2 CD8+ T lymphocytes. LPC caused a dose-dependent intracellular Ca(2+) level ([Ca(2+)](i)) increase in both Jurkat and CTLL-2 lymphocytes. Pretreatment of cells for 5min with 30μM of ruthenium red, a potent ryanodine receptor inhibitor, reduced the LPC-induced Ca(2+) response in both Jurkat and CTLL-2 T lymphocytes. Moreover, pretreatment of cells with 100μM 2-APB for 15min, a cell-permanent IP(3) receptor inhibitor, reduced about two thirds of the LPC induced calcium response in both kinds of cells. However, preincubation of the cells with verapamil, an L-type Ca(2+) channel blocker, did not affect the LPC-induced [Ca(2+)](i) increase in CTLL-2 lymphocytes but inhibited this in Jurkat lymphocytes by 26%. In Ca(2+)-free medium, LPC produced 75.8% of the total [Ca(2+)](i) increase in CTLL-2 lymphocytes and 38% of the total [Ca(2+)](i) increase in Jurkat lymphocytes. These data suggested that the LPC-induced [Ca(2+)](i) increase in human Jurkat and mouse CTLL-2 cell lines occurs via different pathways.
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China.
Qi
Q
Wang
Yi-Jun
YJ
Wu
D000818:Animals; D001896:Boron Compounds / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000037:antagonists & inhibitors; D019169:Jurkat Cells; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D051379:Mice; D012430:Ruthenium Red / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D001896:Boron Compounds; D002121:Calcium Channel Blockers; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008244:Lysophosphatidylcholines; D012430:Ruthenium Red; D014700:Verapamil; C109986:2-aminoethoxydiphenyl borate; D002118:Calcium