Combining flagelliform and dragline spider silk motifs to produce tunable synthetic biopolymer fibers.
The two Flag/MaSp 2 silk proteins produced recombinantly were based on the basic consensus repeat of the dragline silk spidroin 2 protein (MaSp 2) from the Nephila clavipes orb weaving spider. However, the proline-containing pentapeptides juxtaposed to the polyalanine segments resembled those found in the flagelliform silk protein (Flag) composing the web spiral: (GPGGX(1) GPGGX(2))(2) with X(1) /X(2) = A/A or Y/S. Fibers were formed from protein films in aqueous solutions or extruded from resolubilized protein dopes in organic conditions when the Flag motif was (GPGGX(1) GPGGX(2))(2) with X(1) /X(2) = Y/S or A/A, respectively. Post-fiber processing involved similar drawing ratios (2-2.5×) before or after water-treatment. Structural (ssNMR and XRD) and morphological (SEM) changes in the fibers were compared to the mechanical properties of the fibers at each step. Nuclear magnetic resonance indicated that the fraction of β-sheet nanocrystals in the polyalanine regions formed upon extrusion, increased during stretching, and was maximized after water-treatment. X-ray diffraction showed that nanocrystallite orientation parallel to the fiber axis increased the ultimate strength and initial stiffness of the fibers. Water furthered nanocrystal orientation and three-dimensional growth while plasticizing the amorphous regions, thus producing tougher fibers due to increased extensibility. These fibers were highly hygroscopic and had similar internal network organization, thus similar range of mechanical properties that depended on their diameters. The overall structure of the consensus repeat of the silk-like protein dictated the mechanical properties of the fibers while protein molecular weight limited these same properties. Subtle structural motif re-design impacted protein self-assembly mechanisms and requirements for fiber formation.
Department of Biology, Utah State University, Logan, UT 84322-5305, USA. florence.teule@usu.edu
Florence
F
Teulé
Bennett
B
Addison
Alyssa R
AR
Cooper
Joel
J
Ayon
Robert W
RW
Henning
Chris J
CJ
Benmore
Gregory P
GP
Holland
Jeffery L
JL
Yarger
Randolph V
RV
Lewis
D000409:Alanine / Q000737:chemistry; D020816:Amino Acid Motifs; D000818:Animals; D001696:Biomechanical Phenomena; D001704:Biopolymers / Q000737:chemistry*; D004548:Elasticity; D005349:Fibroins / Q000737:chemistry* / Q000648:ultrastructure; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011392:Proline / Q000737:chemistry; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000648:ultrastructure; D012996:Solutions; D013112:Spiders / Q000502:physiology*; D013718:Tensile Strength / Q000502:physiology*; D014867:Water; D014961:X-Ray Diffraction
D001704:Biopolymers; D011993:Recombinant Fusion Proteins; D012996:Solutions; D014867:Water; D005349:Fibroins; D011392:Proline; D000409:Alanine