The use of a library of industrial materials to determine the nature of substrate-dependent performance of primary adherent human cells.
We developed a library of industrial materials, which can be applied to any adherent cell type for determining cell-material interactions. Bulk and surface chemistry as well as other material properties were characterized. The library covered broad ranges of various material properties. We applied the library to primary human endothelial and epithelial cells, which play important roles in tissue engineering and biomedical applications. The results revealed that substrate stiffness was the major determinant of cell performance. The ability to grow and differentiate on stiff or more compliant materials was cell type-dependent, but cell performance was consistently best on stiff and smooth materials. These results give new insights into the nature of substrate-dependent performance of primary human cells and are potentially useful for the development of improved biomaterials. The materials of the library can be easily accessed by the scientific community to determine cell-material interactions of any adherent cell type of interest.
Institute of Bioengineering and Nanotechnology, The Nanos, Singapore 138669, Singapore.
Ming
M
Ni
Philipp K
PK
Zimmermann
Karthikeyan
K
Kandasamy
Weizheng
W
Lai
Yao
Y
Li
Meng Fatt
MF
Leong
Andrew C A
AC
Wan
Daniele
D
Zink
D001672:Biocompatible Materials / Q000737:chemistry*; D035843:Biomedical Research; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D004548:Elasticity; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008855:Microscopy, Electron, Scanning; D020959:Polyethylene; D016062:Porosity; D060888:Real-Time Polymerase Chain Reaction; D018629:Spectrometry, Mass, Secondary Ion; D017550:Spectroscopy, Fourier Transform Infrared; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D020959:Polyethylene