Real-time monitoring of extracellular matrix-mediated PC12 cell attachment and proliferation using an electronic biosensing device.
The attachment and proliferation of a well-established, neuron-like cell line, rat pheochromocytoma (PC12) cells, on different extracellular matrices (ECMs) was monitored using cellular impedance sensing (CIS). Commonly used ECMs, including fibronectin, laminin, poly-L: -lysine, collagen and poly-L: -lysine followed by laminin, in addition to DMEM cell culture media alone as a control, were studied: CIS identified the dynamic progress of the adhesion and proliferation of the cells on different ECMs. Among these modified ECM surfaces, the laminin- and poly-L: -lysine/laminin-modified surfaces were the best suited for the neuron-to-electrode surface attachment and proliferation, which was confirmed by MTT assays and a scanning electron microscopy analysis. This work provides a simple method to study neuron cell/ECM interactions in a real-time, label-free, and quantitative manner.
Medical Systems Biology Research Center, Department of Biomedical Engineering, Tsinghua University School of Medicine, Haidian District, Beijing, 100084, China.
Yan
Y
Zhang
Lei
L
Wang
Jing
J
Zhu
Yadong
Y
Hu
Wanli
W
Xing
Jing
J
Cheng
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002448:Cell Adhesion; D049109:Cell Proliferation; D017097:Electric Impedance; D005109:Extracellular Matrix / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors
D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue