[Study on methodology of determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma by LC-MS/MS].
OBJECTIVE
To develop a sensitive and specific LC-MS/MS method for determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma.


METHODS
Beagle dog plasma pre-treatment methods were established. Geniposide, notoginsenoside R1, ginsenoside Rg1 and Rb1 notoginsenoside molecular ions and fragment ions peaks were separated well and detected synchronously by LC-MS/MS with digoxin as internal standard.


RESULTS
Under the selected LC-MS/MS conditions, the characteristic fragment ions of the four components could be well separated and quantified, and the calibration curves showed good linearity within a certain concentration range of each component; extraction recoveries of those four compounds in plasma were higher than 75%, method recoveries were higher than 90%; day precision (RSD <10%) and inter-day precision (RSD <15%) were generally fine; freeze-thaw and room temperature experiments showed good stability (RSD <15%).


CONCLUSIONS
The method is proved to be suitable for pharmacokinetic studies of Yizhi active ingredients in Beagle dog plasma.
Tianjin University of Chinese Traditional Medicine, Tianjin 300193, China. wymingming@163.com
Yanming
Y
Wang
Shouying
S
Du
Yongsong
Y
Zhai
Yang
Y
Lu
D032429:Alpinia; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D009995:Osmosis; D010936:Plant Extracts; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature; D013997:Time Factors
C442779:Alpinia oxyphylla fruit extract; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D013607:Tablets