Steady state pig dendritic cells migrating in skin draining pseudo-afferent lymph are semi-mature.
Dendritic cells (DC) in peripheral tissues are considered as immature cells that mature and migrate towards lymph nodes upon stimulation with pathogens. This commonly accepted paradigm is challenged by the fact that tolerance to peripheral self antigen is controlled by mature DC and that DC collected from afferent lymph draining different tissues from several species, in the absence of pathogen signaling, were inconsistently found to be either at a mature or semi-mature state. In order to better define the maturation state of DC that migrate in lymph in absence of pathogen stimulation, we compared skin lymph DC to resident and LPS (lipopolysaccharide)-activated skin DC thanks to the establishment of a mini-pig model of lymph duct cannulation. Based on their co-stimulatory molecules expression and endocytotic capacities, pig lymph skin DC were found at an intermediate state of maturation between resident and LPS-activated skin DC and were fully capable of allogeneic T cell stimulation. Furthermore, lymph skin DC could be further matured by LPS or influenza stimulation. Thus, using the pig skin model which is relevant to human, we show that skin-derived DC constantly migrate at an intermediate state of maturation that can be further enhanced upon appropriate stimulation.
Virologie et Immunologie Moléculaires UR892, Institut National de Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France. nicolas.bertho@jouy.inra.fr
Nicolas
N
Bertho
Florian
F
Marquet
Florentina
F
Pascale
Chantal
C
Kang
Michel
M
Bonneau
Isabelle
I
Schwartz-Cornil
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology; D002465:Cell Movement / Q000276:immunology / Q000502:physiology; D007801:Langerhans Cells / Q000276:immunology / Q000502:physiology*; D010641:Phenotype; D012867:Skin / Q000166:cytology / Q000276:immunology; D013552:Swine / Q000276:immunology*