Isolation and identification of antitumor promoters from the seeds of Cassia tora.
A methanol extract of Cassia tora seeds was successively partitioned with diethyl ether, chloroform, ethyl acetate, and water, and the antitumor-promoting activity of the solvent fractions was determined by inhibition of Epstein- Barr virus early antigen (EBV-EA) activation induced by teleocidin B-4 in Raji cells. The diethyl ether (68.7%) and chloroform (91.2%) fractions and the hydrolysate (94.3%) of the ethyl acetate fraction had strong inhibitory activities. The chloroform and ethyl acetate fractions were chromatographed on silica gel and further purified by HPLC. Three active compounds, obtusifolin-2-glucoside (75.0%), chryso-obtusin-6-glucoside (56.8%), and norrubrofusarin- 6-glucoside (39.4%), were obtained from the ethyl acetate fraction, and two active compounds, questin (97.9%) and chryso-obtusin (53.8%), were isolated from the chloroform fraction.
Department of Food Processing and Bakery, Gangwon Provincial College, Gangnung 210-804, Korea.
Yeung-Beom
YB
Park
Seon-Bong
SB
Kim
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002366:Cassia / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts