Verotoxin-2 activates mitogen-activated protein kinases in bovine adherent peripheral blood mononuclear cells.
The effects of verotoxin (VT) on the mitogen-activated protein (MAP) kinase signalling pathways were investigated in bovine adherent peripheral blood mononuclear cells (PBMCs). VT2 stimulated a transient activation of both p38 MAP kinase and extracellular-regulated kinase (ERK) and stimulated an increase in tumour necrosis factor-α release from PBMCs. Bovine PBMCs react with very similar kinetics to human peripheral blood monocytes, despite the gross differences in disease outcome of the two species on infection with verotoxigenic Escherichia coli.
Food Pathogens Group, Division of Bacteriology, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, UK. pamela.cameron@moredun.ac.uk
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Cameron
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Paton
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Smith
D000818:Animals; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis*; D054884:Host-Pathogen Interactions; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000201:enzymology; D022641:Shiga Toxin 2 / Q000633:toxicity*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis*
D022641:Shiga Toxin 2; D014409:Tumor Necrosis Factor-alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases