[Metabonomic phenotype of "formula corresponding to pattern types" based on "qi and yin deficiency pattern" of myocardial ischemia rat model].
In order to explore the scientific connotation of "Fangzhengduiying (formula corresponding to pattern types)", "Qiyinliangxuzheng (Qi and Yin deficiency pattern)" of myocardial ischemia rat model and GC-TOF/MS based metabonomic method were used for comparing the effects of Sheng-mai injection, Salvia injection and propranolol in the present study. After data processing and pattern recognition, Sheng-mai injection showed better efficacy than the other two drugs in accordance with not only visual observation from PLS-DA scores plots but also the number of abnormal endogenous compounds restored to the normal level. Further studies showed that Sheng-mai injection could normalize the level of plasma endothelin-1, the index related to cardiovascular diseases and sleep disorders, which verified the results of metabonomics. Finally, the regulated metabolites and related metabolic pathways were analyzed, and it was supposed that the effects of Sheng-mai injection involved in the alternation of energy metabolism, lipid metabolism, amino acids metabolism, and so on. These findings provided scientific evidence to Shengmai "Fang" used for "Qi and Yin deficiency pattern" correspondingly, indicating that metabonomics has great potential in traditional Chinese medical research, which provides a novel approach and way to modernization of traditional Chinese medicine.
Department of Clinical Pharmacology, Beijing Hospital, Ministry of Health, Beijing 100730, China.
Bei
B
Yan
Ji-Ye
JY
A
Hai-Ping
HP
Hao
Guang-Ji
GJ
Wang
Lin-Sheng
LS
Liu
Wei-Bin
WB
Zha
Ying
Y
Zhang
Sheng-Hua
SH
Gu
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D019332:Endothelin-1 / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007267:Injections; D008297:Male; D008516:Medicine, Chinese Traditional; D055432:Metabolomics / Q000379:methods; D017202:Myocardial Ischemia / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011433:Propranolol / Q000494:pharmacology; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley; D027544:Salvia / Q000737:chemistry; D029633:Schisandraceae / Q000737:chemistry; D016710:Yin Deficiency / Q000378:metabolism*
D000889:Anti-Arrhythmia Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; D011433:Propranolol