Bacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNA(Phe) with 3,4-dihydroxy-L-phenylalanine.
Aminoacyl-tRNA synthetases exert control over the accuracy of translation by selective pairing the correct amino acids with their cognate tRNAs, and proofreading the misacylated products. Here we show that three existing, structurally different phenylalanyl-tRNA synthetases-human mitochondrial (HsmtPheRS), human cytoplasmic (HsctPheRS), and eubacterial from Thermus thermophilus (TtPheRS), catalyze mischarging of tRNA(Phe) with an oxidized analog of tyrosine-L-dopa. The lowest level of L-dopa discrimination over the cognate amino acid, exhibited by HsmtPheRS, is comparable to that of tyrosyl-tRNA synthetase. HsmtPheRS and TtPheRS complexes with L-dopa revealed in the active sites an electron density shaping this ligand. HsctPheRS and TtPheRS possessing editing activity are capable of hydrolyzing the exogenous L-dopa-tRNA(Phe) as efficiently as Tyr-tRNA(Phe). However, editing activity of PheRS does not guarantee reduction of the aminoacylation error rate to escape misincorporation of L-dopa into polypeptide chains.
Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia.
Nina
N
Moor
Liron
L
Klipcan
Mark G
MG
Safro
D020134:Catalytic Domain; D003593:Cytoplasm / Q000201:enzymology; D056890:Eukaryota / Q000201:enzymology*; D006801:Humans; D007980:Levodopa / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D010652:Phenylalanine-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D017393:RNA Editing; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D016963:Thermus thermophilus / Q000201:enzymology*; D014443:Tyrosine / Q000031:analogs & derivatives
D012346:RNA, Transfer, Amino Acyl; D014443:Tyrosine; D007980:Levodopa; D010652:Phenylalanine-tRNA Ligase