Resveratrol inhibits cell differentiation in 3T3-L1 adipocytes via activation of AMPK.
Resveratrol (Res) is a natural polyphenolic compound with anti-inflammatory and antioxidant properties. Also, Res can inhibit lipogenesis and adipocyte differentiation. However, the underlying mechanisms of Res's functions remain largely unknown. AMP-activated protein kinase (AMPK) is a key player in adipocyte differentiation. Therefore, the purpose of our study was to determine the role played by AMPK in the Res-mediated regulation of adipocyte differentiation. Incubation of 3T3-L1 cells with Res confirmed that Res inhibited adipocyte differentiation. The phosphorylation of AMPKα was increased by Res in a dose-dependent manner, while total AMPKα levels were unchanged, and peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1c (SREBP-1c) levels were decreased. Interestingly, pretreatment with AMPKα siRNA and Res promoted adipocyte differentiation, while the decrease of p-AMPKα increased PPARγ, C/EBPα, and SREBP-1c protein expression. Our study shows that Res is capable of inhibiting lipogenesis and differentiation of 3T3-L1 adipocytes via activation of AMPK, suggesting its potential therapeutic application in the treatment or prevention of obesity.
Department of Toxicology, Guangzhou Center for Disease Control and Prevention, P. R. China.
Sifan
S
Chen
Zilun
Z
Li
Wenxue
W
Li
Zhiming
Z
Shan
Wei
W
Zhu
D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000187:drug effects / Q000378:metabolism*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D050155:Lipogenesis / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000503:physiopathology; D047495:PPAR gamma / Q000187:drug effects / Q000502:physiology*; D010766:Phosphorylation; D000077185:Resveratrol; D051780:Sterol Regulatory Element Binding Protein 1 / Q000187:drug effects / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D051780:Sterol Regulatory Element Binding Protein 1; D013267:Stilbenes; D055372:AMP-Activated Protein Kinases; D000077185:Resveratrol