Alkaline phosphatase enzymatic signal amplification for fast, sensitive impedimetric DNA detection.
Enzymatic signal amplification by the deposition of insoluble product on the electrode surface enhances impedimetric DNA detection sensitivity. This work demonstrates a method which gives the required detection sensitivity at significantly reduced enzyme reaction times, and demonstrates the capability for DNA SNP discrimination of biologically relevant sequences. This opens up the prospect of more rapid and relevant multiparameter impedimetric bioassays.
University of Edinburgh, College of Medicine and Veterinary Medicine, Division of Pathway Medicine, 49 Little France Crescent, Edinburgh, UK.
Miriam
M
Kaatz
Holger
H
Schulze
Ilenia
I
Ciani
Fred
F
Lisdat
Andrew R
AR
Mount
Till T
TT
Bachmann
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004247:DNA; D000469:Alkaline Phosphatase