Procyanidins from Japanese quince (Chaenomeles japonica) fruit induce apoptosis in human colon cancer Caco-2 cells in a degree of polymerization-dependent manner.
Plant proanthocyanidins, including procyanidins, display various biological activities. Here we report an inhibition of human colon cancer Caco-2 cell growth by the extract from Japanese quince fruit and the procyanidin-rich fractions of the extract. We observed that the amount of apoptotic Caco-2 cells increased by 52.1% vs. control after 72-h incubation with 50 μg extract/mL, as assessed by flow cytometry and image cytometry. Under the same experimental conditions the corresponding values for human colon cancer HT-29 cells and for rat normal intestinal IEC-6 cells were 5.0% and 8.1%, respectively. The extract fractions enriched with higher oligomers exhibited the highest proapoptotic activity. In conclusion, the Japanese quince procyanidins exhibited proapoptotic activity in Caco-2 cells within a submilimolar concentration range.
Institute of Technical Biochemistry, Technical University of Lodz, Lodz, Poland.
Sylwia
S
Gorlach
Waldemar
W
Wagner
Anna
A
Podsędek
Karolina
K
Szewczyk
Maria
M
Koziołkiewicz
Jarosław
J
Dastych
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells; D002453:Cell Cycle; D002460:Cell Line; D005638:Fruit / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D058105:Polymerization; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D027824:Rosaceae / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D044945:Proanthocyanidins