Hypothyroid states mitigate the diabetes-induced reduction of calbindin D-28k, calretinin, and parvalbumin immunoreactivity in type 2 diabetic rats.
In this study, we investigated the differences in calbindin D-28k (CB), calretinin, (CR) and parvalbumin (PV) immunoreactivity in the hippocampus of Zucker diabetic fatty (ZDF) rats and Zucker lean control (ZLC) rats. In addition, we observed the effects of hypothyroidism on the levels of immunoreactivity of these proteins in ZDF rats. For this study, 7-week-old ZDF rats were used, and methimazole treatment was continued for 5 weeks to induce hypothyroidism. The animals were sacrificed at 12 weeks of age. ZDF rats showed increased blood glucose levels compared to those in ZLC rats. Methimazole intervention significantly reduced total and free T3 levels, and it ameliorated the increase of blood glucose levels in ZDF rats. In ZLC rats, CB, CR, and PV immunoreactivity was detected in regions of the hippocampus proper. In vehicle-treated ZDF rats, CB, CR, and PV immunoreactivity was significantly decreased in the hippocampus. However, in the methimazole-treated rats, CB, CR, and PV immunoreactivity was significantly increased compared to that in the vehicle-treated rats. These results suggest that hypothyroidism ameliorated the diabetes-induced reduction of CB, CR, and PV immunoreactivity in the hippocampus.
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
Sung Min
SM
Nam
Yo Na
YN
Kim
Dae Young
DY
Yoo
Sun Shin
SS
Yi
Woosuk
W
Kim
In Koo
IK
Hwang
Je Kyung
JK
Seong
Yeo Sung
YS
Yoon
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D064032:Calbindin 2; D064026:Calbindins; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007037:Hypothyroidism; D007150:Immunohistochemistry; D008297:Male; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013963:Thyroid Hormones / Q000097:blood
D001786:Blood Glucose; C576512:Calb2 protein, rat; D064032:Calbindin 2; D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D013963:Thyroid Hormones