Neuroprotective effect of transcorneal electrical stimulation on ischemic damage in the rat retina.
Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotective effect and further investigated its underlying mechanism. Adult female Sprague-Dawley (SD) rats received TES treatment every other day after ocular ischemia was induced by elevating the intraocular pressure to 120 mm Hg for 60 min. Retinal ganglion cells (RGCs) were labeled retrogradely 7 days before ischemia and were counted 7 and 14 days later. At the same time points, retinal function was assessed by scotopic electroretinography (ERG), combined with retinal histological analysis. The glutamine synthetase (GS) immunoreactivity was compared between ischemic retinas with TES and those with sham stimulation under identical confocal laser microscope conditions. The immunohistochemical indications were confirmed by Western blot analysis. Higher mean density of RGCs was quantified in TES treated retinas compared to retinas with sham stimulation on days 7 and 14 after ischemia. Similarly, histological analysis showed that TES better preserved the mean thickness of separate retinal layers. ERG studies indicated that by undergoing TES treatment, the b-wave amplitude was also significantly preserved on day 7 after ischemia and recovered robustly on day 14. Immunohistochemical and Western blot analysis both revealed that GS levels remarkably increased after TES and lasted for at least 7 days. Our results indicate that TES can protect retinal neurons against ischemic insults, probably related to increasing levels of GS localized in Müller cells. These findings suggest a new approach for potential clinical application to ocular ischemic diseases.
Department of Ophthalmology and Vision Sciences and Key Laboratory of Myopia of State Health Ministry, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China.
Xin
X
Wang
Xiaofen
X
Mo
Dake
D
Li
Yan
Y
Wang
Yuan
Y
Fang
Xianfang
X
Rong
Huamao
H
Miao
Tiande
T
Shou
D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002470:Cell Survival / Q000502:physiology; D003315:Cornea / Q000502:physiology; D003623:Dark Adaptation; D004599:Electric Stimulation Therapy / Q000379:methods*; D004596:Electroretinography; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D018613:Microscopy, Confocal; D010775:Photic Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy*; D012160:Retina / Q000503:physiopathology; D012164:Retinal Diseases / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy*; D012165:Retinal Ganglion Cells / Q000502:physiology
D005974:Glutamate-Ammonia Ligase