Modulatory effect of L-glutamine on 2,3,7,8 tetrachlorodibenzo-p-dioxin-induced liver injury in rats.
The aim of this study was to explore the effectiveness of L-glutamine (Gln) in alleviating the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in liver of rats. Rats were intraperitoneally administered Gln and TCDD doses daily for 21 days. In the liver of rats, the biochemical tests, pathological examination and micronucleus (MN) test were performed. TCDD significantly decreased the activities of antioxidant enzymes and serious pathological findings. Moreover, the rate of MNs in hepatocytes increased after treatment with dioxin. In rats treated with Gln alone, the MNs remained unchanged, but the ratio of glutathione (GSH) and the activity of glutathione peroxidase (GSH-Px) were significantly increased. Gln also prevented the suppression of GSH-Px (except for superoxide dismutase and catalase) and GSH in the livers of animals exposed to TCDD and displayed a strong protective effect against MNs. Thus, our findings for Gln might provide new insight into the development of therapeutic and preventive approaches in TCDD toxicity.
Department of Biology, Atatürk University, Erzurum, Turkey.
Hasan
H
Türkez
Fatime
F
Geyikoglu
Mokhtar I
MI
Yousef
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology*; D005973:Glutamine / Q000494:pharmacology*; D006651:Histocytochemistry; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D008297:Male; D015162:Micronucleus Tests; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D000072317:Polychlorinated Dibenzodioxins; D005973:Glutamine; D002374:Catalase; D013482:Superoxide Dismutase