Differences in Yes-associated protein and mRNA levels in regenerating             liver and hepatocellular carcinoma.
Yes-associated protein (YAP) has been identified as an oncoprotein that             regulates organ size by modulating proliferative and apoptotic activities. The             aim of this study was to measure YAP gene expression and protein levels during             rat liver regeneration and in liver cancer, and to explore possible correlations             between YAP levels and cancer cell proliferation following partial hepatectomy.             We examined YAP expression in rat regenerating liver using real-time PCR, Western             blotting and immunohistochemical methods. YAP mRNA and protein levels were measured             in human hepatocellular carcinoma. The results demonstrated that YAP protein levels             were markedly increased in the regenerating liver (2.5-3 times), while YAP mRNA             levels decreased slightly (1.47-2.17 times) (P=0.017). YAP mRNA and protein levels             were both higher in hepatocellular carcinoma than in para-cancerous tissue. In             conclusion, liver regeneration alone elevated YAP protein levels without elevating             YAP mRNA levels. YAP mRNA and protein levels were both elevated in liver cancer             cells.
Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China.
Cheng
C
Wang
Lin
L
Zhang
Qian
Q
He
Xiaobin
X
Feng
Jin
J
Zhu
Zhaolong
Z
Xu
Xiaojun
X
Wang
Fei
F
Chen
Xiaowu
X
Li
Jiahong
J
Dong
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
D048868:Adaptor Proteins, Signal Transducing; D010750:Phosphoproteins; D012333:RNA, Messenger; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human