Effects of albumin administration in serum liver enzymes of rats in the presence of extrahepatic biliary obstruction.
OBJECTIVE
To study the influence of albumin on changes of liver function in the extrahepatic biliary obstruction through an experimental model in rats.


METHODS
Sixty rats were divided into four groups: Group C (Control): 6 animals. Group M (Fictitious Operation): 18 rats underwent laparotomy and handling of the bile ducts; Groups O (extrahepatic biliary obstruction) and A (Treated with 2% albumin): 18 animals in each group underwent ligation of the ductus liver; The animals in groups M, O and A were divided into three subgroups of 6 animals each to be killed in the 7, 14 and 21 days postoperative (POD). Blood was drawn for determination of total bilirubin (TB), indirect bilirubin (IB), direct bilirubin (DB), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT).


RESULTS
On POD 7, BI levels were 4.5 mg / dl in group O and 2.1 mg / dl in group A (p = 0.025). On the 14th POD, the levels of PA were 1185.2 U / l in the group and O 458.3 U / l in group A (p = 0.004). ALT levels were 101.7 U / l in the group O and 75.7 U / l in group A (= 0.037). On POD 21, the levels of ALP were 1069.5 U / l in the group O and 468.3 U / l in group A (p = 0, 004).


CONCLUSIONS
The administration of albumin reduced the serum levels of bilirubin in the 7th day of supplementation.
School of Medicine, UnB, Brasilia, DF, Brazil.
Jorge Fernando Rebouças
JF
Lessa
Leonardo Siqueira
LS
Rangel
Nilton José de Almeida
NJ
Costa
Orlando
O
Castro e Silva
Carlos Augusto Teixeira da
CA
Cruz
João Batista de
JB
Sousa
D000410:Alanine Transaminase / Q000097:blood; D000418:Albumins / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D017734:Bile Ducts, Extrahepatic; D001663:Bilirubin / Q000097:blood; D001651:Cholestasis, Extrahepatic / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology; D004195:Disease Models, Animal; D007813:Laparotomy; D008026:Ligation / Q000379:methods; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000187:drug effects; D016896:Treatment Outcome
D000418:Albumins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin