Adverse effects of bisphenol A on reproductive physiology in male goldfish at environmentally relevant concentrations.
Alternations of reproductive physiology were studied in the male goldfish (Carassius auratus L.) exposed to environmentally relevant concentrations (0.6, 4.5 and 11.0 μg/L) of bisphenol A (BPA) at days 10, 20 and 30 after exposure. Significant effects of BPA concentration, exposure time and their interactions were observed on testosterone (T), 11-ketotestosterone (11-KT) and sperm motility and velocity, but gonadosomatic index (GSI), hepatosomatic index (HSI) and 17β-estradiol (E(2)) were not affected. Vitellogenin (VTG) was only affected by BPA concentration. The T and 11-KT levels were significantly decreased in the BPA-treated groups after 20 or 30 days. Sperm motility was significantly decreased at 15, 30, 60 and 90 s post-activation in the BPA-treated groups after 20 or 30 days. But, significant decrease in sperm velocity was observed at 30, 60 and 90 s post-activation in the BPA-treated groups at all exposure times. The VTG was significantly increased in the males exposed to 11.0 μg/L at day 30 after exposure. The GSI, HSI and E(2) did not differ between the BPA-treated groups and control. The present study shows that the decrease of sperm quality is concurrent with the decrease of androgens and increase of VTG. The results suggest adverse effects of BPA on sperm motility and velocity via modifications of testicular steroidogenesis that might correspond to alternation in sperm maturation.
South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany 38925, Czech Republic. ahatef@frov.jcu.cz
Azadeh
A
Hatef
Sayyed Mohammad Hadi
SM
Alavi
Abdulbaset
A
Abdulfatah
Pascal
P
Fontaine
Marek
M
Rodina
Otomar
O
Linhart
D000818:Animals; D001559:Benzhydryl Compounds; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity; D004958:Estradiol / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism* / Q000502:physiology; D008297:Male; D010636:Phenols / Q000008:administration & dosage / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects*; D013737:Testis; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D010636:Phenols; D013739:Testosterone; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C003600:11-ketotestosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds