A water soluble tri-cationic porphyrin-EDTA conjugate induces apoptosis in human neuroendocrine tumor cell lines.
In this study, a completely water soluble tri-cationic porphyrin-EDTA conjugate was synthesized. We present data demonstrating the tumoristatic effects of the novel fully water soluble cationic porphyrin TMPy(3)PhenEDTA-P-Cl(4) in the dark, in the medullary thyroid carcinoma cell lines MTC-SK and SHER-I and weaker effects in the small intestinal neuroendocrine tumor cell line KRJ-I. In addition, cytotoxic effects were also studied in normal human fibroblasts that represent normal tissue and the results are compared to the tumor cell lines.
Department of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, A-8010 Graz, Austria.
Gert
G
Schwach
Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok 10330, Thailand.
Patchanita
P
Thamyongkit
Institute of Inorganic Chemistry, Johannes Kepler University Linz (JKU), Altenberger Straße 69, 4040 Linz, Austria.
Lorenz Michael
LM
Reith
Department of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, A-8010 Graz, Austria.
Bernhard
B
Svejda
Institute of Inorganic Chemistry, Johannes Kepler University Linz (JKU), Altenberger Straße 69, 4040 Linz, Austria.
Günther
G
Knör
Department of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, A-8010 Graz, Austria. Electronic address: roswitha.pfragner@medunigraz.at.
Roswitha
R
Pfragner
Institute of Inorganic Chemistry, Johannes Kepler University Linz (JKU), Altenberger Straße 69, 4040 Linz, Austria. Electronic address: wolfgang.schoefberger@jku.at.
Wolfgang
W
Schoefberger
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D018358:Neuroendocrine Tumors / Q000473:pathology; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D014867:Water / Q000737:chemistry
C572064:5,10,15-tris(N-methylpyridinium-4-yl)-20-(1-phenyl-2-(ethylene diamine-tetraacetic acid))-21H,23H-porphyrin; D000970:Antineoplastic Agents; D011166:Porphyrins; D014867:Water; D004492:Edetic Acid; D053148:Caspase 3; D053179:Caspase 7