Actinoplanes utahensis ZJB-08196 fed-batch fermentation at elevated osmolality for enhancing acarbose production.
Acarbose, a potent α-glucosidase inhibitor, is as an oral anti-diabetic drug for treatment of the type two, noninsulin-dependent diabetes. Actinoplanes utahensis ZJB-08196, an osmosis-resistant actinomycete, had a broad osmolality optimum between 309 mOsm kg(-1) and 719 mOsm kg(-1). Utilizing this unique feature, an fed-batch culture process under preferential osmolality was constructed through intermittently feeding broths with feed medium consisting of 14.0 g l(-1) maltose, 6.0 g l(-1) glucose and 9.0 g l(-1) soybean meal, at 48 h, 72 h, 96 h and 120 h. This intermittent fed-batch culture produced a peak acarbose titer of 4878 mg l(-1), increased by 15.9% over the batch culture.
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
Ya-Jun
YJ
Wang
Li-Ling
LL
Liu
Yuan-Shan
YS
Wang
Ya-Ping
YP
Xue
Yu-Guo
YG
Zheng
Yin-Chu
YC
Shen
D020909:Acarbose / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology; D008320:Maltose / Q000494:pharmacology; D008848:Micromonosporaceae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D009994:Osmolar Concentration; D012970:Sodium Glutamate / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D008320:Maltose; D005947:Glucose; D020909:Acarbose; D012970:Sodium Glutamate