Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils.
The purpose of this study was to explore the potential modulation of equine neutrophil oxidative burst by a series of classical NSAIDs which was subsequently monitored by the luminol or lucigenin-enhanced chemiluminescence (CL) technique. A significant dose-dependent inhibition of the luminol CL was observed with the majority of investigated drugs. This inhibition was very significant for phenylbutazone and Indomethacin; while for aspirin, a higher concentration is required. The action of Ketoprofen was significant during the first 5 min and only when the concentration was above 1 mM. Indomethacin and acetylsalicylic acid result in an inhibition dose-dependent of luminol CL. On the other hand, the phenylbutazone showed an inhibiting effect when used either luminol or lucigenin though luminol is slightly better. When the ketoprofen is considered, an inhibiting effect of luminal CL was observed but less significant than the other NSAIDs investigated. The flunixin meglumine enhances strongly the CL.
University of Mascara, 29000, Mascara, Algeria. benbarekh@yahoo.com
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Benbarek
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Ayad
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Deby-Dupont
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Boukraa
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Serteyn
D000166:Acridines / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006736:Horses / Q000276:immunology*; D008163:Luminescent Measurements / Q000662:veterinary; D008165:Luminol / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D016897:Respiratory Burst / Q000187:drug effects
D000166:Acridines; D000894:Anti-Inflammatory Agents, Non-Steroidal; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol