[Effects and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted rats].
OBJECTIVE
To investigate the effect and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted SD rats.


METHODS
After the obese model was built,Sibiraea angustata was administrated intragastrically to obese rats for 8 weeks. Peeled off fat around kidneys and made pathological tissue sections. The number and size of adipocytes were detected. The levels of adiponectin, adipoR2, AMPK, and PPARgamma mRNA in adipose tissue were detected by RT-PCR. AMPK protein expression in adipose tissue were detected by Western Blot.


RESULTS
Compared with the model group, the diameter of adipocytes were reduced while the number increased after adminiseration of Sibiraea angustata for 8 weeks. The levels of adiponectin, adipoR2, AMPK and PPARgamma mRNA were increased siginficantly. The expression of AMPK protein was also up-regulated significantly.


CONCLUSIONS
Sibiraea angustata has anti-obesity effect. The mechanism may be related to the adiponectin signal transduction pathway.
Department of Pathology and Pathophysiology, Basic Medical Science of Lanzhou University, Lanzhou 730000, China. xiam08@lzu.cn
Miao
M
Xia
Jing-yu
JY
Wang
Xiao-yan
XY
Ma
Yan-song
YS
Lu
Qing-shan
QS
Tian
Xin
X
Liu
D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027824:Rosaceae / Q000737:chemistry*; D015398:Signal Transduction
D052242:Adiponectin; D019440:Anti-Obesity Agents; D004041:Dietary Fats; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D055372:AMP-Activated Protein Kinases