One-step casting of Laemmli discontinued sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel.
A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can be cast at the same time because the lower gel contains 10% glycerol, which generates higher density in the lower gel than in the upper gel.
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA. wu.xiaoqiang451@gmail.com
Xiaoqiang
X
Wu
Hisashi
H
Koiwa
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D029968:Escherichia coli Proteins / Q000302:isolation & purification; D005782:Gels; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors; D014325:Tromethamine
D029968:Escherichia coli Proteins; D005782:Gels; C088816:Laemmli buffer; D014325:Tromethamine; D012967:Sodium Dodecyl Sulfate