Novel flaviviruses detected in different species of mosquitoes in Spain.
We report the characterization of three novel flaviviruses isolated in Spain. Marisma Mosquito virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc. caspius and Culex pipiens, respectively. During this investigation, we designed a sensitive RT-nested polymerase chain reaction method that amplifies a 1019bp fragment of the flavivirus NS5 gene and could be directly used in clinical or environmental samples for flavivirus characterization and surveillance. Analysis of the sequence generated from that amplicon contains enough phylogenetic information for proper taxonomic studies. Moreover, the use of this tool allowed the detection of additional flavivirus DNA forms in Culex, Culiseta, and Ochlerotatus mosquitoes.
Laboratory of Arboviruses and Imported Viral Diseases, National Center for Microbiology, Instituto de Salud Carlos III-ISCIII, Madrid, Spain. a.vazquez@isciii.es
Ana
A
Vázquez
María-Paz
MP
Sánchez-Seco
Gustavo
G
Palacios
Francisca
F
Molero
Noelia
N
Reyes
Santiago
S
Ruiz
Carles
C
Aranda
Eduard
E
Marqués
Raul
R
Escosa
Juana
J
Moreno
Jordi
J
Figuerola
Antonio
A
Tenorio
D000818:Animals; D001483:Base Sequence; D003465:Culex / Q000821:virology; D009033:Culicidae / Q000821:virology*; D005260:Female; D005416:Flavivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018177:Flavivirus Infections / Q000635:transmission; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D044969:Ochlerotatus / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D017361:Viral Nonstructural Proteins / Q000235:genetics*
C096041:NS5 protein, flavivirus; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins