Ultra-selective and sensitive DNA detection by a universal apurinic/apyrimidinic probe-based endonuclease IV signal amplification system.
A novel signal amplification system that is applicable to any DNA sequence of interest has been developed by using a combination of apurinic/apyrimidinic probe and endonuclease IV. The system allows rapid, highly selective and sensitive detection of target DNA sequences at very low concentrations (10 fmol) or low abundance levels (1%).
Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xianjin
X
Xiao
Chen
C
Song
Chen
C
Zhang
Xin
X
Su
Meiping
M
Zhao
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry*; D043223:Deoxyribonuclease IV (Phage T4-Induced) / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence
C114557:5-carboxytetramethylrhodamine succinimidyl ester; D015342:DNA Probes; D012235:Rhodamines; D004247:DNA; D043223:Deoxyribonuclease IV (Phage T4-Induced)