Lactobacillus brantae sp. nov., isolated from faeces of Canada geese (Branta canadensis).
Three strains of lactic acid bacteria (LAB) were isolated from the faeces of apparently healthy wild Canada geese (Branta canadensis) in 2010 by cultivating faecal LAB on Rogosa SL agar under aerobic conditions. These three isolates were found to share 99.9 % gene sequence similarity of their 16S rRNA, their 16S-23S intergenic transcribed spacer region (ITS), partial 23S rRNA, rpoB, rpoC, rpoA and pheS gene sequences. However, the three strains exhibited lower levels of sequence similarity of these genetic targets to all known LAB, and the phylogenetically closest species to the geese strains were Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus saniviri. In comparison to L. casei ATCC 393(T), L. paracasei ATCC 25302(T), L. rhamnosus ATCC 7469(T) and L. saniviri DSM 24301(T), the novel isolates reacted uniquely in tests for cellobiose, galactose, mannitol, citric acid, aesculin and dextrin, and gave negative results in tests for l-proline arylamidase and l-pyrrolydonyl-arylamidase, and in the Voges-Proskauer test. Biochemical tests for cellobiose, aesculin, galactose, gentiobiose, mannitol, melezitose, ribose, salicin, sucrose, trehalose, raffinose, turanose, amygdalin and arbutin could be used for differentiation between L. saniviri and the novel strains. On the basis of phenotypic and genotypic characteristics, and phylogenetic data, the three isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus brantae sp. nov. is proposed. The type strain is SL1108(T) (= ATCC BAA-2142(T) = LMG 26001(T) = DSM 23927(T)) and two additional strains are SL1170 and SL60106.
Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM-470, Rockville, MD 20852, USA.
Dmitriy V
DV
Volokhov
American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA 20110, USA.
Megan
M
Amselle
American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA 20110, USA.
Brian J
BJ
Beck
Department of Biological Sciences, Virginia Tech, Life Sciences I - MC0910, Blacksburg, VA 24061, USA.
David L
DL
Popham
Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD 20740, USA.
Paul
P
Whittaker
Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD 20740, USA.
Hua
H
Wang
American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA 20110, USA.
Elizabeth
E
Kerrigan
Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM-470, Rockville, MD 20852, USA.
Vladimir E
VE
Chizhikov
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005777:Geese / Q000382:microbiology*; D005798:Genes, Bacterial; D005838:Genotype; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D002241:Carbohydrates; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S