Comparison of several mathematical models for describing the joint effect of temperature and ph on glucanex activity.
The aim of the present work was to evaluate with different statistical criteria the suitability of nine equations for describing and optimizing the simultaneous effect of temperature and pH on glucanex activity using two characteristic polysaccharides (curdlan and laminarin) as substrates. The most satisfactory solutions were found with an empirical equation constituted with parameters of practical interest (Rosso model), and a hybrid model between the Arrhenius equation and the mathematical expression generated by the protonation-hydroxylation mechanism (Tijskens model). The joint optimal values of pH and temperature calculated with the Rosso model were obtained at 4.64 and 50°C with curdlan and 4.64 and 48°C using laminarin as substrate.
Grupo de Reciclado e Valorización de Materiais Residuais (REVAL), Instituto de Investigacións Mariñas (CSIC), r/Eduardo Cabello, 6. Vigo-36208, Galicia, Spain. michaelumangelum@iim.csic.es
Miguel Angel
MA
Prieto
José Antonio
JA
Vázquez
Miguel Anxo
MA
Murado
D055162:Biocatalysis; D004795:Enzyme Stability; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism; D005936:Glucans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008962:Models, Theoretical; D011134:Polysaccharides / Q000378:metabolism; D013696:Temperature; D047071:beta-Glucans / Q000378:metabolism
D005936:Glucans; D011134:Polysaccharides; D047071:beta-Glucans; C038459:curdlan; C008247:laminaran; D043326:Glucan 1,3-beta-Glucosidase