Cryptococcus thermophilus sp. nov., isolated from cassava sourdough.
A novel anamorphic yeast, strain LTH 6662(T), was isolated from cassava sourdough. The isolate supposedly originated from cassava flour or was a contaminant thereof. Sequencing of the D1/D2 domain of the 26S rRNA gene indicated that strain LTH 6662(T) represents a novel species. Its closest relatives were members of the Cryptococcus humicola complex. The novel strain had several physiological characteristics that differed from those of related species: the ability to assimilate raffinose and cadaverine; the inability to assimilate soluble starch, xylitol, galactitol, butane-2,3-diol, sodium nitrite and lysine; the ability to grow without vitamins and at 42 °C; and the inability to produce starch-like substances. Its major ubiquinone was Q-10. In addition, buds were formed on small neck-like structures. In liquid medium, green or blue fluorescent substances were produced. The name Cryptococcus thermophilus sp. nov. is proposed, with LTH 6662(T) (=DSM 19443(T)=CBS 10687(T)) as the type strain.
Institute of Food Science and Biotechnology, University of Hohenheim, 70599 Stuttgart, Germany.
Stephanie A
SA
Vogelmann
Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Edificio ICAT, Campus da FCUL, 1749-016 Lisboa, Portugal.
Sandra
S
Chaves
Frutarom Savory Solutions GmbH, 70825 Korntal-Münchingen, Germany.
Christian
C
Hertel
D016000:Cluster Analysis; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D020459:Genes, rRNA; D002365:Manihot; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
D003470:Culture Media; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D014451:Ubiquinone