Study of the potential toxicity of enniatins A, A(1), B, B(1) by evaluation of duodenal and colonic bioavailability applying an in vitro method by Caco-2 cells.
The bioavailability of the minor Fusarium mycotoxins enniatins (ENs) utilizing an in vitro method which allows the simulation of the small and large intestine tracts has been studied. This method, based on the application of the Caco-2 cells grown alone or in symbiosis with several strains characteristics of the gastrointestinal tract, has permitted to simulate the duodenal and colonic intestinal compartments, respectively. The duodenal bioavailability expressed as absorption value after 4h of exposure, ranged from 57.7 to 76.8% for EN A, from 68.8 to 70.2% for EN A(1), from 65.0 to 67.0% for EN B, and from 62.2 to 65.1% for EN B(1). Colonic bioavailability after 48h of incubation ranged from 17.3 to 33.3% for EN A, from 40.8 to 50.0% for EN A(1), from 47.7 to 55.0% for EN B, and from 52.4 to 57.4% for En B(1). The highest duodenal and colonic bioavailability was observed after Caco-2 cells exposed to EN A, ENs B and B(1), respectively.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Avenue Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain. giuseppe.meca@uv.es
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D001682:Biological Availability; D018938:Caco-2 Cells; D003106:Colon / Q000378:metabolism; D047630:Depsipeptides / Q000493:pharmacokinetics / Q000633:toxicity*; D004386:Duodenum / Q000378:metabolism; D005670:Fusarium; D006801:Humans; D007408:Intestinal Absorption; D009183:Mycotoxins / Q000493:pharmacokinetics / Q000633:toxicity*; D018570:Risk Assessment; D018675:Toxicity Tests / Q000379:methods
D047630:Depsipeptides; D009183:Mycotoxins; C100264:enniatins