Ionic liquids based microwave-assisted extraction of lichen compounds with quantitative spectrophotodensitometry analysis.
Ionic liquids based extraction method has been applied to the effective extraction of norstictic acid, a common depsidone isolated from Pertusaria pseudocorallina, a crustose lichen. Five 1-alkyl-3-methylimidazolium ionic liquids (ILs) differing in composition of alkyl chain and anion were investigated for extraction efficiency. The extraction amount of norstictic acid was determined after recovery on HPTLC with a spectrophotodensitometer. The proposed approaches (IL-MAE and IL-heat extraction (IL-HE)) have been evaluated in comparison with usual solvents such as tetrahydrofuran in heat-reflux extraction and microwave-assisted extraction (MAE). The results indicated that both the characteristics of the alkyl chain and anion influenced the extraction of polyphenolic compounds. The sulfate-based ILs [C(1)mim][MSO(4)] and [C(2)mim][ESO(4)] presented the best extraction efficiency of norstictic acid. The reduction of the extraction times between HE and MAE (2 h-5 min) and a non-negligible ratio of norstictic acid in total extract (28%) supports the suitability of the proposed method. This approach was successfully applied to obtain additional compounds from other crustose lichens (Pertusaria amara and Ochrolechia parella).
Equipe PNSCM Produits naturels-Synthèses-Chimie Médicinale, UMR CNRS 6226 Sciences chimiques de Rennes, UFR Sciences Pharmaceutiques et Biologiques, Université Rennes 1, Université Européenne de Bretagne, Rennes, France.
Sarah
S
Bonny
Ludovic
L
Paquin
Daniel
D
Carrié
Joël
J
Boustie
Sophie
S
Tomasi
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003720:Densitometry / Q000379:methods*; D052578:Ionic Liquids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D008011:Lichens / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008872:Microwaves; D013053:Spectrophotometry / Q000379:methods
D052578:Ionic Liquids