Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid.
The C-terminal domain of Nipah virus (NiV) nucleocapsid protein (NP₄₀₁₋₅₃₂) was inserted at the N-terminus and the immunodominant loop of hepatitis B core antigen (HBc). The stability of NP₄₀₁₋₅₃₂ increased tremendously when displayed on the HBc particles. These particles reacted specifically with the swine anti-NiV and the human anti-HBc antisera.
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Wei Boon
WB
Yap
Beng Ti
BT
Tey
Noorjahan Banu Mohamed
NB
Alitheen
Wen Siang
WS
Tan
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000276:immunology; D006512:Hepatitis B Core Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000378:metabolism*; D006801:Humans; D045405:Nipah Virus / Q000276:immunology*; D019590:Nucleocapsid Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine
D000956:Antigens, Viral; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins