Analysis of proteomic changes in roots of soybean seedlings during recovery after flooding.
A proteomic approach was used to identify proteins involved in post-flooding recovery in soybean roots. Two-day-old soybean seedlings were flooded with water for up to 3 days. After the flooding treatment, seedlings were grown until 7 days after sowing and root proteins were then extracted and separated using two-dimensional polyacrylamide gel electrophoresis (2-DE). Comparative analysis of 2-D gels of control and 3 day flooding-experienced soybean root samples revealed 70 differentially expressed protein spots, from which 80 proteins were identified. Many of the differentially expressed proteins are involved in protein destination/storage and metabolic processes. Clustering analysis based on the expression profiles of the 70 differentially expressed protein spots revealed that 3 days of flooding causes significant changes in protein expression, even during post-flooding recovery. Three days of flooding resulted in downregulation of ion transport-related proteins and upregulation of proteins involved in cytoskeletal reorganization, cell expansion, and programmed cell death. Furthermore, 7 proteins involved in cell wall modification and S-adenosylmethionine synthesis were identified in roots from seedlings recovering from 1 day of flooding. These results suggest that alteration of cell structure through changes in cell wall metabolism and cytoskeletal organization may be involved in post-flooding recovery processes in soybean seedlings.
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
Afshin
A
Salavati
Amana
A
Khatoon
Yohei
Y
Nanjo
Setsuko
S
Komatsu
D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism*; D013025:Glycine max / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*
D010940:Plant Proteins