Rational design of fluorescent biosensor for cyclic di-GMP.
Messenger bagged: The design of a fluorophore-labeled protein biosensor for the bacterial messenger cyclic di-GMP is described. The biosensor responds to c-di-GMP with sub-micromolar sensitivity in a real-time fashion. The biosensor can be used for enzyme assays for diguanylate cyclases and c-di-GMP phosphodiesterases as well as the high-throughput screening of inhibitors.
Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore.
Chun Loong
CL
Ho
Siew Lee
SL
Koh
Mary Lay Cheng
ML
Chuah
Zhen
Z
Luo
Wei Jian
WJ
Tan
Donovan Kian Soon
DK
Low
Zhao-Xun
ZX
Liang
D015374:Biosensing Techniques; D006152:Cyclic GMP / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D006152:Cyclic GMP