Synergistic effect of thermal energy on bactericidal action of photolysis of H₂O₂ in relation to acceleration of hydroxyl radical generation.
The purpose of the present study is to evaluate the effect of thermal energy on the yield of and the bactericidal action of hydroxyl radical generated by photolysis of H(2)O(2). Different concentrations of H(2)O(2) (250, 500, 750, and 1,000 mM) were irradiated with light-emitting diodes (LEDs) at a wavelength of 400 ± 20 nm at 25°C to generate hydroxyl radical. The 500 mM H(2)O(2) was irradiated with the LEDs at different temperatures (25, 35, 45, and 55°C). Electron spin resonance spin trapping analysis showed that the yield of hydroxyl radicals increased with the temperature, as well as the concentration of H(2)O(2). Streptococcus mutans and Enterococcus faecalis were used in the bactericidal assay. The LED-light irradiation of the bacterial suspensions in 500 mM H(2)O(2) at 25°C could hardly kill the bacteria within 3 min, while the bactericidal effect was markedly enhanced with the temperature rise. For instance, a temperature increase to 55°C resulted in >99.999% reduction of viable counts of both bacterial species only within 1 min. The photolysis of 500 mM H(2)O(2) at 55°C could reduce the viable counts of bacteria more efficiently than did the photolysis of 1,000 mM H(2)O(2) at 25°C, although the yields of hydroxyl radical were almost the same under the both conditions. These findings suggest that the thermal energy accelerates the generation of hydroxyl radical by photolysis of H(2)O(2), which in turn results in a synergistic bactericidal effect of hydroxyl radical and thermal energy.
Division of Fixed Prosthodontics, Department of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Seiryou 4-1, Aoba-ku, Sendai 980-8575, Japan.
Midori
M
Shirato
Hiroyo
H
Ikai
Keisuke
K
Nakamura
Eisei
E
Hayashi
Taro
T
Kanno
Keiichi
K
Sasaki
Masahiro
M
Kohno
Yoshimi
Y
Niwano
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism*; D054023:Lasers, Semiconductor; D008027:Light; D050296:Microbial Viability / Q000187:drug effects*; D010782:Photolysis / Q000528:radiation effects; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide