Size-dependent immune-modulating effect of amorphous nanosilica particles.
The immune-modulating effect following intradermal injection of various-sized amorphous silica particles was analyzed in terms of induction of ovalbumin-specific CD8+ T cells in vivo. IFN-gamma ELISPOT assays revealed that only nanosilica particles with a diameter of less than 100 nm significantly enhanced CD8+ T cell responses against ovalbumin. These results indicate that the size of nanomaterials is a critical determinant in terms of their safe use.
Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
T
T
Hirai
T
T
Yoshikawa
H
H
Nabeshi
T
T
Yoshida
S
S
Tochigi
M
M
Uji
K
K
Ichihashi
T
T
Akase
T
T
Yamashita
K
K
Yamashita
K
K
Nagano
Y
Y
Abe
H
H
Kamada
S
S
Tsunoda
Y
Y
Yoshioka
N
N
Itoh
Y
Y
Tsutsumi
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D005260:Female; D007155:Immunologic Factors; D007371:Interferon-gamma; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D010047:Ovalbumin / Q000276:immunology; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007155:Immunologic Factors; D012822:Silicon Dioxide; D007371:Interferon-gamma; D010047:Ovalbumin