Effect of glyceric acid calcium salt on the viability of ethanol-dosed gastric cells.
D-Glyceric acid (D-GA) calcium has been reported to accelerate ethanol oxidation in vivo in rats (Eriksson et al., Metabolism, 56, 895-898 (2007)). However, no other reports have shown that D-GA can reduce the harmful effects of ethanol. In this study, the effects of D-, L-, and DL-GA calcium on ethanol-dosed gastric cell viability were investigated using human gastric carcinoma cells (Kato III) and normal rat gastric mucosa cells (RGM1). Addition of 2% and 3 % ethanol to Kato III and RGM1 cells, respectively, decreased their cell viability by approximately 20-50 % after 24 or 72 h of cultivation. In 2 % ethanol-dosed Kato III cells cultivated for 24 h, addition of 0.002-20 µg/mL D- and L-GA calcium did not affect cell viability. Similarly, addition of less than 20 µg/mL DL-GA calcium did not affect cell viability. However, when 20 µg/mL DL-GA calcium was added, cell viability increased by 35.7 % after 72 h of incubation, compared to the viability of control cells without ethanol or GA. Addition of 20 µg/mL DL-GA calcium to 3 % ethanol-dosed RGM1 cells cultivated for 24 or 72 h also increased cell viability up to those observed in control cells. These results suggest that a racemic mixture of GA may have the strongest effect on enhancing the viability of ethanol-exposed cells.
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Japan. hiroshi.habe@aist.go.jp
Hiroshi
H
Habe
Shun
S
Sato
Tokuma
T
Fukuoka
Dai
D
Kitamoto
Keiji
K
Sakaki
D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005988:Glyceric Acids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D012492:Salts / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
D005988:Glyceric Acids; D012492:Salts; D000431:Ethanol; C042971:glyceric acid; D002118:Calcium