In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans.
The in vivo analysis of the roles of splicing factors in regulating alternative splicing in animals remains a challenge. Using a microarray-based screen, we identified a Caenorhabditis elegans gene, tos-1, that exhibited three of the four major types of alternative splicing: intron retention, exon skipping, and, in the presence of U2AF large subunit mutations, the use of alternative 3' splice sites. Mutations in the splicing factors U2AF large subunit and SF1/BBP altered the splicing of tos-1. 3' splice sites of the retained intron or before the skipped exon regulate the splicing pattern of tos-1. Our study provides in vivo evidence that intron retention and exon skipping can be regulated largely by the identities of 3' splice sites.
State Key Laboratory of Medical Genetics, School of Biological Sciences and Technology, Central South University, Changsha, Hunan, China.
Long
L
Ma
Zhiping
Z
Tan
Yanling
Y
Teng
Sebastian
S
Hoersch
H Robert
HR
Horvitz
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005091:Exons; D005786:Gene Expression Regulation; D023061:Gene Order; D007438:Introns; D009154:Mutation / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics; D000072260:RNA Splicing Factors; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D022821:RNA Splice Sites; D000072260:RNA Splicing Factors; D012261:Ribonucleoproteins; D014157:Transcription Factors; C486185:UAF-1 protein, C elegans; C491515:sfa-1 protein, C elegans