Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide.
We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, "SSSWWSWWWW" (SW1) or "SWWWWSWWWW" (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1 μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤0.5 μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.
Toyota Central R&D Lab, Nagakute, Aichi 480-1192, Japan. e1215@mosk.tytlabs.co.jp
Takashi
T
Matsuyama
Kim
K
Domyoung
Mitsuo
M
Umetsu
Taiji
T
Ikawa
Mamoru
M
Yamanishi
Nobuhiro
N
Ishida
Izumi
I
Kumagai
Haruo
H
Takahashi
D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D013449:Sulfonamides / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*; D014867:Water / Q000737:chemistry
C517865:1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide; D007093:Imidazoles; D052578:Ionic Liquids; D010455:Peptides; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D014867:Water; D049452:Green Fluorescent Proteins; D014364:Tryptophan