Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle.
Skeletal muscle lipid droplet-associated proteins (PLINs) are thought to regulate lipolysis through protein-protein interactions on the lipid droplet surface. In adipocytes, PLIN2 [adipocyte differentiation-related protein (ADRP)] is found only on lipid droplets, while PLIN5 (OXPAT, expressed only in oxidative tissues) is found both on and off the lipid droplet and may be recruited to lipid droplet membranes when needed. Our purpose was to determine whether PLIN5 is recruited to lipid droplets with contraction and to investigate the myocellular location and colocalization of lipid droplets, PLIN2, and PLIN5. Rat solei were isolated, and following a 30-min equilibration period, they were assigned to one of two groups: 1) 30 min of resting incubation and 2) 30 min of stimulation (n = 10 each). Immunofluorescence microscopy was used to determine subcellular content, distribution, and colocalization of lipid droplets, PLIN2, and PLIN5. There was a main effect for lower lipid and PLIN2 content in stimulated compared with rested muscles (P < 0.05). Lipid droplet distribution declined exponentially from the sarcolemma to the fiber center in the rested muscles (P = 0.001, r(2) = 0.99) and linearly in stimulated muscles (slope = -0.0023 ± 0.0006, P < 0.001, r(2) = 0.93). PLIN2 distribution declined exponentially from the sarcolemma to the fiber center in both rested and stimulated muscles (P < 0.0001, r(2) = 0.99 rest; P = 0.0004, r(2) = 0.98 stimulated), while PLIN5 distribution declined linearly (slope = -0.0085 ± 0.0009, P < 0.0001, r(2) = 0.94 rest; slope=-0.0078 ± 0.0010, P = 0.0003, r(2) = 0.91 stimulated). PLIN5-lipid droplets colocalized at rest with no difference poststimulation (P = 0.47; rest r(2) = 0.55 ± 0.02, stimulated r(2) = 0.58 ± 0.03). PLIN2-lipid droplets colocalized at rest with no difference poststimulation (P = 0.48; rest r(2) = 0.66 ± 0.02, stimulated r(2) = 0.65 ± 0.02). Contrary to our hypothesis, these results show that PLIN5 is not recruited to lipid droplets with contraction in isolated skeletal muscle.
Brock University, Department of Kinesiology, Centre for Muscle Metabolism and Biophysics, Faculty of Applied Health Sciences, St. Catharines, Ontario, Canada. rm09aa@Brocku.ca
Rebecca E K
RE
MacPherson
Eric A F
EA
Herbst
Erica J
EJ
Reynolds
Rene
R
Vandenboom
Brian D
BD
Roy
Sandra J
SJ
Peters
D000818:Animals; D004558:Electric Stimulation; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D023421:Models, Animal; D009119:Muscle Contraction / Q000502:physiology*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D000070780:Perilipin-2; D000070797:Perilipin-5; D051381:Rats; D020318:Rats, Long-Evans; D012146:Rest / Q000502:physiology*
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; C544353:PLIN5 protein, rat; D000070780:Perilipin-2; D000070797:Perilipin-5; C000602984:Plin2 protein, rat