Evaluation of biological effects of nanomaterials. Part I. Cyto- and genotoxicity of nanosilver composites applied in textile technologies.
OBJECTIVE
The aim of this study was to investigate the cyto- and genotoxicity of nanocomposites (NCs) and generation of reactive oxygen species (ROS) as a result of particle-cell interactions.


METHODS
Titanium dioxide (TiO(2)-Ag) and ion-exchange resin (Res-Ag), both coated with silver (Ag), were examined. The murine macrophage J774A.1 cells were incubated in vitro with NC at different concentrations for 24 h. Cytotoxicity was analyzed by the methylthiazolyldiphenyl-tetrazolium bromide reduction test (MTT reduction test). ROS generation was assessed by incubation of cells with dichlorodihydrofluorescein diacetate (DCF) and flow cytometry. DNA damage was detected by comet assay and included single-strand breaks (SSB), alkali-labile sites (ALS) and oxidative DNA damage after formamidopyrimidine glycosylase (FPG) treatment. The tail moment was used as an indicator of DNA damage.


RESULTS
TiO(2)-Ag was not cytotoxic up to 200 μg/ml, whereas IC(50) for Res-Ag was found to be 23 μg/ml. Intracellular ROS levels were elevated after 4 h of exposure to Res-Ag at the concentration of 50 μg/ml. Both types of NC induced fragmentation of DNA strands, but only one of the composites caused damage to purine bases. TiO(2)-Ag induced SSB of DNA at concentrations of 10 and 5 μg/ml. For Res-Ag, a concentration-dependent increase in tail moments was observed.


CONCLUSIONS
Silver-coated nanocomposites (both TiO(2)-Ag and Res-Ag) may cause genotoxic effects in murine macrophages J774A.1. Res-Ag increased generation of ROS which suggested that toxicity of Res-Ag in murine macrophages is likely to be mediated through oxidative stress. This paper will support industry and regulators alike in the assessment of hazards and risks and methods for their mitigation at the earliest possible stage in material and product development.
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Łódź, Poland. wojciech@imp.lodz.pl
Wojciech
W
Wąsowicz
Małgorzata
M
Cieślak
Jadwiga
J
Palus
Małgorzata
M
Stańczyk
Elżbieta
E
Dziubałtowska
Maciej
M
Stępnik
Markus
M
Düchler
D001681:Biological Assay / Q000379:methods; D003603:Cytotoxins / Q000032:analysis* / Q000235:genetics / Q000506:poisoning*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D009153:Mutagens / Q000032:analysis* / Q000506:poisoning*; D049329:Nanostructures / Q000506:poisoning*; D016273:Occupational Exposure / Q000009:adverse effects / Q000032:analysis; D011044:Poland; D017382:Reactive Oxygen Species / Q000032:analysis; D012834:Silver / Q000009:adverse effects* / Q000378:metabolism*; D013783:Textile Industry
D003603:Cytotoxins; D009153:Mutagens; D017382:Reactive Oxygen Species; D012834:Silver