Fibrin glue mixed with platelet-rich fibrin as a scaffold seeded with dental bud cells for tooth regeneration.
Odontogenesis is a complex process with a series of epithelial-mesenchymal interactions and odontogenic molecular cascades. In tissue engineering of teeth from stem cells, platelet-rich fibrin (PRF), which is rich in growth factors and cytokines, may improve regeneration. Accordingly, PRF was added into fibrin glue to enrich the microenvironment with growth factors. Unerupted second molar tooth buds were harvested from miniature swine and cultured in vitro for 3 weeks to obtain dental bud cells (DBCs). Whole blood was collected for the preparation of PRF and fibrin glue before surgery. DBCs were suspended in fibrin glue and then enclosed with PRF, and the DBC-fibrin glue-PRF composite was autografted back into the original alveolar sockets. Radiographic and histological examinations were used to identify the regenerated tooth structure 36 weeks after implantation. Immunohistochemical staining was used to detect proteins specific to tooth regeneration. One pig developed a complete tooth with crown, root, pulp, enamel, dentin, odontoblast, cementum, blood vessels, and periodontal ligaments in indiscriminate shape. Another animal had an unerupted tooth that expressed cytokeratin 14, dentin matrix protein-1, vascular endothelial growth factor, and osteopontin. This study demonstrated, using autogenic cell transplantation in a porcine model, that DBCs seeded into fibrin glue-PRF could regenerate a complete tooth.
Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
Kai-Chiang
KC
Yang
Chun-Hao
CH
Wang
Hao-Hueng
HH
Chang
Wing P
WP
Chan
Chau-Hwa
CH
Chi
Tzong-Fu
TF
Kuo
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001792:Blood Platelets; D002454:Cell Differentiation; D003782:Dental Pulp / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D005337:Fibrin / Q000737:chemistry / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive / Q000737:chemistry / Q000494:pharmacology*; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013552:Swine; D014014:Tissue Adhesives / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014070:Tooth; D014182:Transplantation, Autologous
D000943:Antigens, Differentiation; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D005337:Fibrin