Cytochrome c dissolved in 1-allyl-3-methylimidazolium chloride type ionic liquid undergoes a quasi-reversible redox reaction up to 140°C.
Solubility of cytochrome c, a typical heme protein, in a various ionic liquids has been analyzed. The solubility has been discussed with polarity parameters of the ionic liquids. Both hydrogen bond basicity and dipolarity/polarizability of the ionic liquids were confirmed to be influential factors to control the solubilization of cytochrome c. Polar ionic liquids such as 1-butyl-3-methylimidazolium chloride and 1-allyl-3-methylimidazolium chloride solubilized cytochrome c at 80°C, and the dissolved cytochrome c was found to keep its redox activity in these ionic liquids. The redox response of the dissolved cytochrome c was detected in 1-allyl-3-methylimidazolium chloride up to 140°C.
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan.
Kaori
K
Tamura
Nobuhumi
N
Nakamura
Hiroyuki
H
Ohno
D000498:Allyl Compounds / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism*; D006358:Hot Temperature; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D012995:Solubility
C544334:1-allyl-3-methylimidazolium; D000498:Allyl Compounds; D002712:Chlorides; D007093:Imidazoles; D052578:Ionic Liquids; D045304:Cytochromes c; D010100:Oxygen