The association of cytochrome P450 1B1 Leu432Val polymorphism with biological markers of health and menopausal symptoms in Slovak midlife women.
OBJECTIVE
In this study, the CYP1B1 polymorphism was examined in relationship to recognized risk factors for cardiovascular disease. In particular, this study focused on plasma lipid levels, atherogenic indices, and body composition. Furthermore, this polymorphism was analyzed as a predisposing factor for menopausal symptoms among women during midlife, subdivided according to their menopause status.


METHODS
A total of 399 women aged from 39 to 60 years were examined. They were recruited from the western and middle parts of Slovakia. Participants were interviewed during their medical examination, and they were investigated with respect to a variety of aspects such as anthropometric and medical aspects, and a menopause-specific questionnaire was included. The participants provided a saliva or blood sample for DNA genotyping and a blood sample for biochemical analysis.


RESULTS
The Leu432Val genotype demonstrated statistically significant associations with triglycerides, with the ratio of total cholesterol to high-density lipoprotein cholesterol, and with the logarithm of the ratio of plasma concentration of triglycerides to high-density lipoprotein cholesterol in women in their reproductive period. The mean values were significantly lower in women carrying the Val/Val genotype. Four atherogenic indices showed a decreasing trend in relationship to the CYP1B1 genotypes in women during their reproductive period (in the following order of magnitude: Leu/Leu + Leu/Val vs Val/Val) and an increasing trend among postmenopausal women in the same order. Furthermore, the Val/Val genotype diminished experiences of bloated stomach, of vaginal dryness in perimenopausal and postmenopausal women, and of palpitations in premenopausal women.


CONCLUSIONS
The Leu432Val polymorphism may be associated with the lipid profile in midlife women. Moreover, this polymorphism may influence the risk of some menopausal symptoms.
Department of Anthropology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. luptakova@fns.uniba.sk
Lenka
L
Luptáková
Daniela
D
Sivaková
Dagmara
D
Srámeková
Marta
M
Cvíčelová
D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D050197:Atherosclerosis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis*; D001823:Body Composition; D065633:Cytochrome P-450 CYP1B1; D004247:DNA / Q000032:analysis / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood / Q000235:genetics; D008593:Menopause / Q000235:genetics* / Q000502:physiology; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012463:Saliva / Q000737:chemistry; D018154:Slovakia; D011795:Surveys and Questionnaires; D014280:Triglycerides / Q000097:blood / Q000235:genetics
D015415:Biomarkers; D008055:Lipids; D008075:Lipoproteins, HDL; D014280:Triglycerides; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1