Study on permeability of DMSO in embryos of red seabream (Pagrus major) by capillary electrophoresis.
The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5 to 40 percent, volume basis) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (less than 50 percent) after exposure in 35 percent, 20 percent and 15 percent DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was greater 50 percent, the internal DMSO concentration was less than 2 percent, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.
Center of Biotechnology R and D, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, P.R. China.
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F
Lin
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Liu
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Xiao
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Ma
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Xu
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Xiao
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J
Li
D000818:Animals; D017756:Aquaculture / Q000379:methods; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D008297:Male; D010539:Permeability; D021541:Sea Bream / Q000196:embryology* / Q000502:physiology*; D013997:Time Factors
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide