A cluster of specified microRNAs in peripheral blood as biomarkers for metastatic non-small-cell lung cancer by stem-loop RT-PCR.
OBJECTIVE
To investigate the levels for some specified microRNAs in human's peripheral blood so as to determine whether they can serve as biomarkers for metastatic non-small-cell lung cancer.


METHODS
Use a quantitative stem-loop RT-PCR method to examine the serum levels for certain microRNAs including has-miR-125a-5p, has-miR-126, has-miR-183, has-miR-200, has-miR-221, and has-miR-222 from the patients with Stage IV, Stage I/II non-small-cell lung cancer and the controls.


RESULTS
There was statistical difference in the serum levels for hsa-miR-126, hsa-miR-183, and hsa-miR-222 between the controls and the Stage IV patients, but not for has-miR-125a-5p, has-miR-200 and has-miR-221. It also showed statistical difference for hsa-miR-126 and hsa-miR-183 between the Stage I/II patients and Stage IV patients, but not between the controls and Stage I/II patients.


CONCLUSIONS
Hsa-miR-126 and hsa-miR-183 may serve as potential serum biomarkers for metastatic non-small-cell lung cancer.
Oncology Department, Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China.
Qingfeng
Q
Lin
Weidong
W
Mao
Yongqian
Y
Shu
Feng
F
Lin
Shaoping
S
Liu
Hua
H
Shen
Wen
W
Gao
Suqing
S
Li
Dong
D
Shen
D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D014408:Biomarkers, Tumor; D035683:MicroRNAs