[Investigation of inflammasome during excitation of IgG-HepG2 cells for evaluation of allergenic ingredients].
OBJECTIVE
To investigate the alteration of inflammasome and receptor during IgG promoter transfected to HepG2 cells.


METHODS
By assay of Elisa to evaluate the secretion of IL-1 beta, IL-8, TNF-alpha and MCP-1 after puerarine and LPS administration, and by assay of real time PCR to evaluate the expression of mRNA of IL-1 beta, IL-8,TNF-alpha and MCP-1, as well as the receptors of TLR2, 4 and NOD2, MyD88.


RESULTS
IgG promoter did not active innate immunity and enhance the expression and secretion of inflammasome in HepG2. Puerarine did not active the inflammasome either. LPS activated the innate immunity and increased the secretion of IL-8, TNF-alpha and MCP-1.


CONCLUSIONS
IgG-HepG2 cells could be used specifically as the model of allergy type II for ingredients screening. It is suggested that puerarine was suite for the activator for this type of allergy as positive control.
Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
Xi
X
Lu
Mingwei
M
Wang
Fan
F
Lei
Yugang
Y
Wang
Zhiyi
Z
Yuan
Shuang
S
Zhao
Huiyu
H
Li
Dongming
D
Xing
Lijun
L
Du
D000485:Allergens / Q000032:analysis* / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005786:Gene Expression Regulation / Q000276:immunology; D056945:Hep G2 Cells; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007074:Immunoglobulin G / Q000235:genetics*; D058847:Inflammasomes / Q000276:immunology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014162:Transfection
D000485:Allergens; D004365:Drugs, Chinese Herbal; D007074:Immunoglobulin G; D058847:Inflammasomes