Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers.
Analyte detection with biosensors is strongly influenced by the preparation of the biosensor surface including choice of sensing layers and coupling methods for corresponding capture molecules. We investigated the influence of different coupling procedures, especially considering coupling chemistry and incubation times for reagents, by means of surface acoustic wave (SAW) biosensors. The effect on the signal response was tested in two subsequent protein assays. Our optimized coupling procedure allowed the detection of the breast cancer markers HER-2 (human epidermal growth factor receptor-2) and TIMP-1 (tissue inhibitor of metalloproteinase-1) below the respective clinical cutoff values of only a few nanograms per milliliter.
Karlsruhe Institute of Technology (KIT), Institute for Microstructure Technology, 76344 Eggenstein-Leopoldshafen, Germany. friederike.gruhl@kit.edu
Friederike J
FJ
Gruhl
Kerstin
K
Länge
D000162:Acoustics; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D019921:Biotinylation; D006801:Humans; D057230:Limit of Detection; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000276:immunology; D013205:Staphylococcal Protein A / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis* / Q000276:immunology
D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; D013205:Staphylococcal Protein A; D019715:Tissue Inhibitor of Metalloproteinase-1