Xenoestrogenic chemicals effectively alter sperm functional behavior in mice.
Xenoestrogenic compounds (XCs) can disrupt endogenous hormone function and affect sperm function by binding to receptors on sperm membrane. Albeit spermatozoa are potentially a useful model for screening estrogenic activities of endocrine disruptors, high-quality in vitro test system that examination of the XCs effects on sperm function is required. The objective of this study was to compare the effects of XCs (genistein and 4-tert-octylphenol) to those of steroids (estrogen and progesterone) and heparin on in vitro capacitation and acrosome reaction (AR) in mouse spermatozoa. Mouse spermatozoa were incubated with various concentrations (0.001-100 μM) of each chemical for 15 or 30 min, and then capacitation and AR were assessed using chlortetracycline. All chemicals studied effectively alter capacitation and/or AR in mouse spermatozoa with different manner. Therefore, we believed that our system will provide a good in vitro model system to characterize the physiological effect of XCs especially when compared with steroids.
Department of Animal Science and Technology, School of Bioresource and Bioscience, Chung-Ang University, Ansung, Gyeonggi-Do, Republic of Korea. legend0508@naver.com
Yoo-Jin
YJ
Park
El-Sayed A
el-SA
Mohamed
Woo-Sung
WS
Kwon
Young-Ah
YA
You
Buom-Yong
BY
Ryu
Myung-Geol
MG
Pang
D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D052244:Endocrine Disruptors / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000494:pharmacology*; D011374:Progesterone / Q000494:pharmacology; D013075:Sperm Capacitation / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology*
D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D011374:Progesterone; D004958:Estradiol; D006493:Heparin; D019833:Genistein; C105260:4-tert-octylphenol