Supercritical fluid chromatography in research laboratories: design, development and implementation of an efficient generic screening for exploiting this technique in the achiral environment.
A new strategy for exploiting SFC in the achiral arena has been designed, developed and successfully implemented in our Analytical Technologies laboratories, in support of Drug Discovery. The development of a generic analytical SFC/MS screening protocol, following extensive evaluation of different stationary phase, modifier and gradient elution combinations, has enabled fast analytical method development for structurally diverse compounds within both specialised and high-throughput purification environments. Application of this approach into the daily routine has provided data on a large number and a wide variety of reaction mixtures. The results confirm the scope and generality of the strategy with more than 85% of targets being successfully resolved from their impurities.
Centro de Investigación Lilly, Avda. de la Industria 30, 28108 Alcobendas, Madrid, Spain. de_la_puente_maria_luz@lilly.com
M L
ML
de la Puente
P
P
López Soto-Yarritu
J
J
Burnett
D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D057166:High-Throughput Screening Assays; D016014:Linear Models; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations