Overexpression of α-enolase correlates with poor survival in canine mammary carcinoma.
BACKGROUND
α-Enolase (ENO1) is a key glycolytic enzyme implicated in the development of many human cancers including breast cancer. Increased expression of ENO1 has recently been reported in estrogen (ER)-positive human breast cancer patients. The present study examined the expression of ENO1 and assessed its significance in canine mammary carcinoma.


RESULTS
Immunohistochemical staining was employed to investigate the expression of ENO1 in 82 cases of canine mammary tumor (32 benign tumors and 50 carcinomas). Quantification of immunohistochemistry was carried out using Quick score and the results showed cytoplasmic ENO1 overexpression in 9 of the 50 carcinomas (18%). Overexpression of ENO1 correlated significantly with shorter cause-specific survival (P = 0.019), but was not associated with ER positivity in canine mammary carcinoma.


CONCLUSIONS
Our findings suggest that overexpression of ENO1 may be used as a prognostic marker for poor outcome in canine mammary carcinoma.
Department of Pathology, St Martin De Porres Hospital, No 565, Section 2, Daya Road, Chiayi 60069, Taiwan.
Pei-Yi
PY
Chu
Nicholas C
NC
Hsu
Albert T
AT
Liao
Neng-Yao
NY
Shih
Ming-Feng
MF
Hou
Chen-Hsuan
CH
Liu
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004283:Dog Diseases / Q000378:metabolism* / Q000401:mortality; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015151:Immunoblotting / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000378:metabolism*
D015415:Biomarkers; D010751:Phosphopyruvate Hydratase