Diversity and polymorphism in AHL-lactonase gene (aiiA) of Bacillus.
To explore bacterial diversity for elucidating genetic variability in acylhomoserine lactone (AHL) lactonase structure, we screened 800 bacterial strains. It revealed the presence of a quorum quenching (QQ) AHL-lactonase gene (aiiA) in 42 strains. These 42 strains were identified using rrs (16S rDNA) sequencing as Bacillus strains, predominantly B. cereus. An in silico restriction endonuclease (RE) digestion of 22 AHL lactonase gene (aiiA) sequences (from NCBI database) belonging to 9 different genera, along with 42 aiiA gene sequences from different Bacillus spp. (isolated here) with 14 type II REs, revealed distinct patterns of fragments (nucleotide length and order) with four REs; AluI, DpnII, RsaI, and Tru9I. Our study reflects on the biodiversity of aiiA among Bacillus species. Bacillus sp. strain MBG11 with polymorphism (115Alanine > Valine) may confer increased stability to AHL lactonase, and can be a potential candidate for heterologous expression and mass production. Microbes with ability to produce AHL-lactonases degrade quorum sensing signals such as AHL by opening of the lactone ring. The naturally occurring diversity of QQ molecules provides opportunities to use them for preventing bacterial infections, spoilage of food, and bioremediation.
Microbial Biotechnology and Genomics, Institute of Genomics and Integrative Biology, CSIR, Delhi University Campus, Delhi-110007, India.
Nusrat
N
Huma
Pratap
P
Shankar
Jyoti
J
Kushwah
Ashish
A
Bhushan
Jayadev
J
Joshi
Tanmoy
T
Mukherjee
Sajan
S
Raju
Hemant J
HJ
Purohit
Vipin Chandra
VC
Kalia
D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D044822:Biodiversity; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012988:Soil Microbiology
D001426:Bacterial Proteins; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase