Stathmin expression in glioma-derived microvascular endothelial cells: a novel therapeutic target.
The purpose of this study was to investigate stathmin expression and its             mechanisms of action in GDMEC. Microvascular endothelial cells were isolated from             human gliomas (n=68) and normal brain specimans (n=20), and purified by magnetic             beads coated with anti-CD105 antibody. The expression of stathmin mRNA and protein             were detected by RT-PCR and western blotting, respectively. Stathmin expression             was silenced by application of specific siRNA in high grade GDMEC. The proliferation,             apoptosis and invasion behavior of GDMEC were investigated. The stathmin positive             rate of endothelial cells in normal brain, grade I-II glioma and grade III-IV             glioma was 20, 66 and 95.5%, respectively (P<0.05). When cells were treated             with siRNA to silence stathmin, cell viability was reduced, the apoptosis rate             increased and the migration of vascular endothelial cells was suppressed significantly             (P<0.05). Down-regulation of stathmin suppressed neoangiogenesis of glioma             and provides a potential target for glioma treatment.
Department of Neurosurgery, the Fourth Affiliated Hospital of Harbin Medical University, and Department of Pathophysiology, Harbin Medical University, Harbin 150001, PR China.
Baijing
B
Dong
Luyan
L
Mu
Xiangying
X
Qin
Wanchen
W
Qiao
Xiaodong
X
Liu
Liming
L
Yang
Li
L
Xue
Nikolai G
NG
Rainov
Xiaoqian
X
Liu
D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005910:Glioma / Q000098:blood supply* / Q000473:pathology; D006801:Humans; D008297:Male; D055806:Microvessels / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D050777:Stathmin / Q000096:biosynthesis* / Q000235:genetics; D055815:Young Adult
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050777:Stathmin