[Evaluation of chromogenic medium Uriselect4 in urine culture].
We evaluated the performance and the cost of chromogenic medium Uriselect4 agar with regard to the standard medium for the detection and identification of urinary tract pathogens. A total of 503 clinical urine specimens containing leucocytes greater or equal to 104/mL were analysed prospectively, in parallel by two different persons on blood agar (GS) and Uriselect4 according to the manufacturers' instructions. Of the 503 urine specimens tested, 210 gave a positive culture on Uriselect4 versus 181 on GS. The majority of bacterial species grew on both media; enterobacteria grew on Uriselect4 better than GS. The identification of Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), KES group and Enterococcus faecalis (E. faecalis) did not require the use of galleries Api and has a gain of 24  h. Positive pure cultures on Uriselect4 corresponding to negative cultures of GS were noted in 17 ases. Conversely, in seven cases a positive pure culture on GS was noted while the corresponding Uriselect4 cultures were negative. The cost of identification on GS (including the cost of galleries Api), was about two times higher than Uriselect4. Uriselect4 medium isolates the most frequent urinary tract pathogens and identify them so almost immediately, with a lower cost.
CHU Farhat Hached, Laboratoire de Microbiologie et D'immunologie UR02SP13, Sousse, Tunisie. aferjeni@lycos.com
Asma
A
Ferjani
Manel
M
Marzouk
Nadia
N
Idriss
Sammoud
S
Sammoud
Naila
N
Hannachi
Janel
J
Boukadida
D000362:Agar / Q000737:chemistry / Q000191:economics*; D001424:Bacterial Infections / Q000382:microbiology / Q000652:urine; D001431:Bacteriological Techniques / Q000379:methods; D001769:Blood / Q000382:microbiology; D002863:Chromogenic Compounds / Q000191:economics; D003116:Color; D003362:Cost-Benefit Analysis; D003470:Culture Media / Q000737:chemistry* / Q000191:economics; D004526:Efficiency; D006801:Humans; D011237:Predictive Value of Tests; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000191:economics / Q000382:microbiology / Q000652:urine*
D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar