Clinical proteomics of the neglected human malarial parasite Plasmodium vivax.
Recent reports highlight the severity and the morbidity of disease caused by the long neglected malaria parasite Plasmodium vivax. Due to inherent difficulties in the laboratory-propagation of P. vivax, the biology of this parasite has not been adequately explored. While the proteome of P. falciparum, the causative agent of cerebral malaria, has been extensively explored from several sources, there is limited information on the proteome of P. vivax. We have, for the first time, examined the proteome of P. vivax isolated directly from patients without adaptation to laboratory conditions. We have identified 153 proteins from clinical P. vivax, majority of which do not show homology to any previously known gene products. We also report 29 new proteins that were found to be expressed in P. vivax for the first time. In addition, several proteins previously implicated as anti-malarial targets, were also found in our analysis. Most importantly, we found several unique proteins expressed by P. vivax.This study is an important step in providing insight into physiology of the parasite under clinical settings.
Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India.
Pragyan
P
Acharya
Rani
R
Pallavi
Syama
S
Chandran
Vrushali
V
Dandavate
Syed Khund
SK
Sayeed
Ankit
A
Rochani
Jyoti
J
Acharya
Sheetal
S
Middha
Sanjay
S
Kochar
Dhanpat
D
Kochar
Susanta Kumar
SK
Ghosh
Utpal
U
Tatu
D000818:Animals; D006801:Humans; D008018:Life Cycle Stages; D008288:Malaria / Q000097:blood / Q000469:parasitology* / Q000517:prevention & control; D017780:Malaria Vaccines / Q000276:immunology; D010966:Plasmodium vivax / Q000187:drug effects / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D060066:Protein Interaction Maps; D040901:Proteomics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
D017780:Malaria Vaccines; D015800:Protozoan Proteins