The chromatographic analysis of oligosaccharides and preparation of 1-kestose and nystose in yacon.
The thin-layer chromatographic analysis of the crude oligosaccharides extracted from yacon revealed the presence of glucose, fructose, sucrose, 1-kestose and nystose. The qualitative and quantitative analysis was carried out on oligosaccharides by high pressure liquid chromatography and the results showed that the contents of d-glucose, fructose, sucrose, 1-kestose, nystose and 1-fructofuranosyl nystose in oligosaccharides were 38.30%, 16.44%, 14.58%, 12.29%, 12.17%, 6.20%, respectively. The content of the fructooligosaccharides in oligosaccharides was 30.66%. The crude oligosaccharides were separated and purified by silica gel column chromatography. The two fractions obtained from crude oligosaccharides were 1-kestose and nystose, which were identified by mass spectra. The yield of 1-kestose and nystose were 10.36% and 9.73%, respectively. The purity of 1-kestose was 82.9% and of nystose was 73.6%.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China.
Zhen-Yuan
ZY
Zhu
Hong-Yu
HY
Lian
Chuan-Ling
CL
Si
Yang
Y
Liu
Nian
N
Liu
Jing
J
Chen
Li-Na
LN
Ding
Qiang
Q
Yao
Yongmin
Y
Zhang
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D009844:Oligosaccharides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014312:Trisaccharides / Q000032:analysis*
D009844:Oligosaccharides; D010936:Plant Extracts; D014312:Trisaccharides; C535244:yacon syrup; C072433:1-kestose; C043600:fungitetraose