Oral immunisation of mice with a recombinant rabies virus vaccine incorporating the heat-labile enterotoxin B subunit of Escherichia coli in an attenuated Salmonella strain.
To investigate effective new rabies vaccines, a fusion protein consisting of the rabies virus (RV) glycoprotein and the heat-labile enterotoxin B subunit of Escherichia coli (LTB) was successfully constructed and delivered in a live attenuated Salmonella strain LH430. Mice were immunised with LH430 carrying pVAX1-G, pVAX1-G-LTB or pVAX1-ori-G-LTB. The antibody titres of mice immunised with oral LH430 carrying pVAX1-G-LTB or pVAX1-ori-G-LTB were significantly higher than those of pVAX1-G-immunised mice. The results of the challenge with the rabies virus standard strain (CVS-11) showed that the LH430 strain carrying the G-LTB gene induced immunity and elevated IL-2 levels in immunised mice ((∗∗)P<0.01), whereas LH430 carrying pVAX1-G did not contribute to protection. These results show that LH430 carrying recombinant G-LTB could provide overall immunity against challenge with CVS-11 and should be considered to be a potential rabies vaccine.
Key Laboratory of Zoonosis, Ministry of Education, Institute for Zoonosis, Jilin University, 5333 Xian Road, 130062 Changchun, PR China.
Xuelin
X
Wang
Juan
J
Liu
Xiuping
X
Wu
Lu
L
Yu
Haiying
H
Chen
Heng
H
Guo
Maolin
M
Zhang
Huiping
H
Li
Xue
X
Liu
Shumin
S
Sun
Lijing
L
Zhao
Xinyue
X
Zhang
Lifang
L
Gao
Mingyuan
M
Liu
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001427:Bacterial Toxins / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D004768:Enterotoxins / Q000737:chemistry* / Q000276:immunology; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology*; D012475:Salmonella / Q000235:genetics / Q000378:metabolism*; D014614:Vaccines, Synthetic / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism
D000914:Antibodies, Viral; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007074:Immunoglobulin G; D011819:Rabies Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; C027538:heat-labile enterotoxin, E coli