Selective photodynamic therapy based on aggregation-induced emission enhancement of fluorescent organic nanoparticles.
Three binary molecule conjugates were designed and synthesized by conjugating a chromophore (3, 6-bis-(1-methyl-4-vinylpyridinium)-carbazole diiodide, BMVC) to mono-, bis- and trishydroxyl photosensitizers, respectively. BMVC plays the role of cancer cells recognizer; AIEE (aggregation-induced emission enhancement) generator and FRET (Fluorescence Resonance Energy Transfer) donor. The self assembling properties of these binary conjugates result in different degrees of AIEE and then achieve the formations of FONs (fluorescent organic nanoparticles), which present efficient FRET and singlet oxygen generations. Biologically, FONs-photosensitizers from these compounds were much more phototoxicities to cancer cell than to normal cell without significant dark toxicity. In addition, their intracellular fluorescent colors switching upon photo-excitation are expected to be used for further cell death biomarker applications. This improved photodynamic activity might be due to the aggregation of compounds in the cell that form FONs which can promote PDT (photodynamic therapy) and are observed in cancer cell but not normal cell.
Graduate Institute of Biomedical Engineering, National Chung Hsing University 250, Taichung 402, Taiwan, ROC. ccchang555@dragon.nchu.edu.tw
Cheng-Chung
CC
Chang
Meng-Chieh
MC
Hsieh
Jung-Chih
JC
Lin
Ta-Chau
TC
Chang
D002460:Cell Line; D015195:Drug Design; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry
D017319:Photosensitizing Agents