Steroidogenesis in amlodipine treated purified Leydig cells.
Drugs have been shown to adversely affect male fertility and recently anti-hypertensive drugs were added to the list. The anti-fertility effects of amlodipine, a calcium channel blocker, are well-illustrated in in vivo experiments but lack an in vitro proof. The present study was designed to experimentally elucidate the effects of amlodipine on Leydig cell steroidogenesis and intracellular calcium in vitro. Leydig cells of Sprague-Dawley rats were isolated and purified by Percoll. Cells were incubated for 3h with/without amlodipine in the presence/absence of LH, dbcAMP, Pregnenolone and 25-Hydroxycholesterol. Cytosolic calcium was measured in purified Leydig cells by fluorometric technique. The results showed significantly reduced (P<0.05) steroidogenesis and intracellular calcium in amlodipine exposed rats. The site of amlodipine induced steroidogenic inhibition seems to be prior to the formation of Pregnenolone at the level of StAR protein.
Department of Physiology, Army Medical College, National University of Sciences and Technology, Islamabad, Pakistan. rabialatif08@hotmail.com
Rabia
R
Latif
Ghulam Mustafa
GM
Lodhi
Waqas
W
Hameed
Muhammad
M
Aslam
D017311:Amlodipine / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000633:toxicity*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000096:biosynthesis*
D002121:Calcium Channel Blockers; D017311:Amlodipine; D013739:Testosterone; D007986:Luteinizing Hormone; D002118:Calcium