Conditional CD8+ T cell escape during acute simian immunodeficiency virus infection.
CD8+ T cell responses rapidly select viral variants during acute human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) infection. We used pyrosequencing to examine variation within three SIV-derived epitopes (Gag₃₈₆₋₃₉₄GW9, Nef₁₀₃₋₁₁₁RM9, and Rev₅₉₋₆₈SP10) targeted by immunodominant CD8+ T cell responses in acutely infected Mauritian cynomolgus macaques. In animals recognizing all three epitopes, variation within Rev₅₉₋₆₈SP10 was associated with delayed accumulation of variants in Gag₃₈₆₋₃₉₄GW9 but had no effect on variation within Nef₁₀₃₋₁₁₁RM9. This demonstrates that the entire T cell repertoire, rather than a single T cell population, influences the timing of immune escape, thereby providing the first example of conditional CD8+ T cell escape in HIV/SIV infection.
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA. doconnor@primate.wisc.edu
Shelby L
SL
O'Connor
Ericka A
EA
Becker
Jason T
JT
Weinfurter
Emily N
EN
Chin
Melisa L
ML
Budde
Emma
E
Gostick
Michael
M
Correll
Michael
M
Gleicher
Austin L
AL
Hughes
David A
DA
Price
Thomas C
TC
Friedrich
David H
DH
O'Connor
D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins