Liposome and epimedium polysaccharide-propolis flavone can synergistically enhance immune effect of vaccine.
Three preparations of epimedium polysaccharide-propolis flavone immunopotentiator (EPI), EPI liposome, EPI suspension and EPI watery solution were prepared. In immune response test, their adjuvanticities were compared in 14-day-old chickens vaccinated with Newcastle disease (ND) vaccine. In immune protection test, the effects of the three preparations on Newcastle disease virus (NDV) infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. The results displayed that EPI liposome could enhance the antibody titer, T lymphocyte proliferation and the concentrations of interferon-γ and interleukin-6, when compared with the other two preparations. In EPI liposome group, the antibody titers, lymphocyte proliferation and protective rate were the highest, while the mortality and morbidity were the lowest, in comparison with the other groups. These results indicated that liposome could enhance the immune effect of EPI on ND vaccine and would be expected as the suitable dosage form of this immunopotentiator.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
Yunpeng
Y
Fan
Deyun
D
Wang
Yuanliang
Y
Hu
Jiaguo
J
Liu
Guocai
G
Han
Xiaojuan
X
Zhao
Ju
J
Yuan
Cui
C
Liu
Xu
X
Liu
Xiang
X
Ni
D000818:Animals; D049109:Cell Proliferation; D002645:Chickens; D034181:Epimedium / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry*; D007107:Immune System; D015850:Interleukin-6 / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry*; D008214:Lymphocytes / Q000166:cytology; D009521:Newcastle Disease / Q000517:prevention & control; D009522:Newcastle disease virus / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D011429:Propolis / Q000737:chemistry*; D014765:Viral Vaccines / Q000627:therapeutic use
D047309:Flavones; D015850:Interleukin-6; D008081:Liposomes; D011134:Polysaccharides; D014765:Viral Vaccines; D011429:Propolis; C043562:flavone