Bioassays guided fractionation of Coronopus didymus for its antifungal activity against Sclerotium rolfsii.
Sclerotium rolfsii Sacc. is a pathogen of about 500 plant species. In a laboratory screening bioassay, methanolic extracts of 15 mg mL⁻¹ concentrations of different parts of Coronopus didymus (L.) Sm. (Brassicaceae) namely leaf, stem, inflorescence and root reduced the biomass of S. rolfsii by 67%, 26%, 40% and 58%, respectively. Methanolic root extract was successively fractionated with n-hexane, chloroform, ethyl acetate and n-butanol. All the concentrations (3.125-200 mg mL⁻¹) of ethyl acetate fraction completely inhibited the target fungal growth. Two compounds A and B were separated from this fraction by Thin Layer Chromatography (TLC). TLC fraction A was found highly effective against S. rolfsii with MIC value 15.62 µg mL⁻¹ as compared to MIC value 7.81 µg mL⁻¹ of the commercial fungicide mencozeb. This compound may be used for the synthesis of natural product based fungicide for the control of S. rolfsii.
Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
Drakshan
D
Iqbal
Arshad
A
Javaid
D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D019607:Brassicaceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D000935:Antifungal Agents; D010936:Plant Extracts