Vero cells infected with the Lederle strain of  canine distemper virus have increased Fas receptor  signaling expression at 15 h post-infection.
We evaluated the expression of the Fas receptor gene  in Vero cells infected with the Lederle vaccine strain of canine distemper  virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in  MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the  virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected  Vero cells were used as a control. Total RNA was isolated from Vero cells using  1 mL Trizol(®) LS, and RT was performed using 2 μg total RNA. Primer pairs for RT-PCR  amplification for the canine distemper virus nucleocapsid gene, the S26  reference gene, and the Vero rFas gene were used to analyze expression in Vero  cells. RT-PCR results revealed virus activity at 3, 6, 9, and 15 h in the  virus-infected Vero cells. The S26 housekeeping gene was amplified in virus  infected and control samples. However, expression of the cell death receptor  Fas was detected in Vero cells only at 15 h post-infection. We suggest that the  Lederle vaccine induces apoptosis by Fas receptor signaling, possibly through  caspase-8 signaling rather than through mitochondrial signaling in the infected  cells.
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. helendelpuerto@hotmail.com
H L
HL
Del Puerto
A S
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Martins
G F
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Braz
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Alves
M B
MB
Heinemann
D S
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Rajão
F C
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Araújo
S F
SF
Martins
D R
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Nascimento
R C
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Leite
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Vasconcelos
D000818:Animals; D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism; D002522:Chlorocebus aethiops; D004216:Distemper / Q000378:metabolism*; D004217:Distemper Virus, Canine / Q000378:metabolism*; D004285:Dogs; D005786:Gene Expression Regulation; D015398:Signal Transduction; D013997:Time Factors; D014709:Vero Cells; D014765:Viral Vaccines / Q000378:metabolism; D019014:fas Receptor / Q000096:biosynthesis*
D014765:Viral Vaccines; D019014:fas Receptor; D053181:Caspase 8