Yeast one-hybrid assays for gene-centered human gene regulatory network mapping.
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
Program in Systems Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA. john.reece-hoyes@umassmed.edu
John S
JS
Reece-Hoyes
A Rasim
AR
Barutcu
Rachel Patton
RP
McCord
Jun Seop
JS
Jeong
Lizhi
L
Jiang
Andrew
A
MacWilliams
Xinping
X
Yang
Kourosh
K
Salehi-Ashtiani
David E
DE
Hill
Seth
S
Blackshaw
Heng
H
Zhu
Job
J
Dekker
Albertha J M
AJ
Walhout
D001665:Binding Sites; D004247:DNA / Q000235:genetics; D053263:Gene Regulatory Networks / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D006801:Humans; D012984:Software; D014157:Transcription Factors / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D014157:Transcription Factors; D004247:DNA