Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.
Endophytic actinobacteria isolated from Artemisia annua were characterized and evaluated for their bioactivities. A total of 228 isolates representing at least 19 different genera of actinobacteria were obtained and several of them seemed to be novel taxa. An evaluation of antimicrobial activity showed that more isolates possessed activity towards plant pathogens than activity against other pathogenic bacteria or yeasts. High frequencies of PCR amplification were obtained for type I polyketide synthases (PKS-I, 21.1%), type II polyketide synthases (PKS-II, 45.2%) and nonribosomal peptide synthetases (NRPS, 32.5%). The results of herbicidal activity screening indicated that 19 out of 117 samples of fermentation broths completely inhibited the germination of Echinochloa crusgalli. This study indicated that endophytic actinobacteria associated with A. annua are abundant and have potentially beneficial and diverse bioactivities which should be pursued for their biotechnical promise.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, People's Republic of China.
Jie
J
Li
Guo-Zhen
GZ
Zhao
Hai-Yu
HY
Huang
Sheng
S
Qin
Wen-Yong
WY
Zhu
Li-Xing
LX
Zhao
Li-Hua
LH
Xu
Si
S
Zhang
Wen-Jun
WJ
Li
Gary
G
Strobel
D039903:Actinobacteria / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D031172:Artemisia annua / Q000382:microbiology*; D001426:Bacterial Proteins / Q000302:isolation & purification; D004269:DNA, Bacterial; D004275:DNA, Ribosomal / Q000235:genetics; D060026:Endophytes / Q000737:chemistry / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D005285:Fermentation; D018525:Germination / Q000187:drug effects; D006540:Herbicides / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000302:isolation & purification; D010802:Phylogeny; D006109:Poaceae / Q000187:drug effects; D048630:Polyketide Synthases / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006540:Herbicides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D048630:Polyketide Synthases; D010453:Peptide Synthases