Microbial preference for different size classes of organic carbon: a study from Antarctic snow.
Significance of carbon cycling in polar ecosystems is well recognized. Yet, bacteria in surface snow have received less attention in terms of their potential in carbon cycling. Here, we present results on carbon utilization by bacterial communities in three surface snow samples from Antarctica collected along a coastal to inland transect. Microcosm studies were conducted over 8 days at 5 ± 1°C to study carbon metabolism in different combinations of added low molecular weight (LMW (glucose, <1 kDa)) and high molecular weight (HMW (starch, >1 kDa)) substrates (final 20 ppm). The total organic carbon (TOC) in the snow samples decreased with time at rates ranging from non-detectable to 1.4 ppm day(-1) with rates highest in snow samples from inland region. In addition, carbon utilization studies were also carried out with bacterial isolates LH1, LH2, and LH4 belonging to the genus Cellulosimicrobium, Bacillus, and Ralstonia, respectively, isolated from the snow samples. Studies with strain LH2 in different amendments of glucose and starch showed that TOC decreased with time in all amendments at a rate of 0.9-1.5 ppm day(-1) with highest rates of 1.4-1.5 ppm day(-1) in amendments containing a higher proportion of starch. The bacterial isolates were also studied to determine their ability to utilize other LMW and HMW compounds. They utilized diverse substrates like carbohydrates, amino acids, amines, amides, complex polymers, etc., of molecular mass <100 Da, 100-500 Da, >500 Da-1 kDa, and >1 kDa preferring (up to 31 times) substrates with mass of >1 kDa than <1 kDa. The ability of bacteria in snow to utilize diverse LMW and HMW substrates indicates that they could be important in the uptake of similar compounds in snow and therefore potentially govern snow chemistry.
National Centre for Antarctic and Ocean Research, Headland Sada, Vasco-da-Gama, Goa 403 804, India. runa@ncaor.org
Runa
R
Antony
K
K
Mahalinganathan
K P
KP
Krishnan
Meloth
M
Thamban
D000864:Antarctic Regions; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D017753:Ecosystem; D059013:Microbial Consortia; D008970:Molecular Weight; D012914:Snow / Q000737:chemistry* / Q000382:microbiology
D002244:Carbon