Pseudahrensia aquimaris gen. nov., sp. nov., isolated from seawater.
A Gram-negative, non-motile, ovoid to rod-shaped bacterium, designated HDW-32(T), was isolated from seawater of the Yellow Sea, Korea. Strain HDW-32(T) grew optimally at pH 7-8, at 30 °C and with 2-3 % (w/v) NaCl. Strain HDW-32(T) exhibited 95.1 % 16S rRNA gene sequence similarity with Nitratireductor basaltis J3(T), 94.8 % sequence similarity with Ahrensia kielensis IAM 12618(T) and <94.5 % with other members of the family Phyllobacteriaceae. In the neighbour-joining, maximum-likelihood and maximum-parsimony trees based on 16S rRNA gene sequences, strain HDW-32(T) clustered with A. kielensis IAM 12618(T). Strain HDW-32(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c as the major fatty acid. Differences in polar lipids, DNA G+C content and other phenotypic properties distinguished strain HDW-32(T) from A. kielensis JCM 20689(T). Strain HDW-32(T) could also be distinguished from representatives of the genera Nitratireductor and Hoeflea by differences in fatty acids and polar lipids. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain HDW-32(T) represents a novel species belonging to a novel genus of the family Phyllobacteriaceae of the class Alphaproteobacteria, for which the name Pseudahrensia aquimaris gen. nov., sp. nov. is proposed. The type strain of the type species is HDW-32(T) ( = KCTC 23345(T) = CCUG 60023(T)).
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon 305-350, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Yong-Taek
YT
Jung
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Sooyeon
S
Park
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Jung-Sook
JS
Lee
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon 306-809, Republic of Korea.
Tae-Kwang
TK
Oh
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10