Boron induced expression of some stress-related genes in tomato.
Boron (B) is a potential environmental toxicant for plants under excessive conditions. To understand the molecular stress response involved in high B exposure, we focused on the transcript accumulation of three stress-related genes: Hsp90, MT2 and GR1. Transcript accumulations were determined on B-stressed tomato plants by using a quantitative real-time PCR technique. Tomato seedlings were exposed to B ranging from 80 to 5120 μM for 24 h in nutrient solution. Root and shoot transcript accumulations were assessed. Results showed that the genes were over-expressed in B-stressed tomato. The highest relative fold change value was measured on GR1 for both root and shoot (8-10 and 30-34-fold increases, respectively), indicating the activation of the oxidative stress enzyme to tolerate B-stress as an early response. The activation of these genes could be a protection mechanism against to B stress.
Department of Biology, Fatih University, 34500 Buyukcekmece, Istanbul, Turkey. htombuloglu@fatih.edu.tr
Huseyin
H
Tombuloglu
Nihan
N
Semizoglu
Serdal
S
Sakcali
Guzin
G
Kekec
D000222:Adaptation, Physiological; D001895:Boron / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D053517:Matrix Metalloproteinase 15 / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D013312:Stress, Physiological / Q000235:genetics*
D018841:HSP90 Heat-Shock Proteins; D012989:Soil Pollutants; D053517:Matrix Metalloproteinase 15; D001895:Boron