[Determination of three sweeteners in vinegars by solid phase extraction-high performance liquid chromatography/tandem mass spectrometry].
A solid phase extraction-high performance liquid chromatography/electrospray ionization-tandem mass spectrometry (SPE-HPLC/ESI-MS/MS) method for the determination of 3 sweeteners (acesulfame (AK), sodium saccharin (SA), sodium cyclamate (SC)) in vinegars has been developed. The sample was diluted with acidic water, then purified and enriched with a weak anion exchange SPE column. The HPLC separation was performed on a Pursuit C18 column (150 mm x 2.0 mm, 3 microm) by gradient elution with 10 mmol/L ammonium acetate containing 0.1% (v/v) ammonia water and acetonitrile as the mobile phases. The analytes were detected by ESI--MS/MS in multiple reaction monitoring (MRM) mode to satisfy qualitative and quantitative detections. Good linearities (r2 > 0.99) were obtained over the range of 0.01 - 0.50 mg/L. The limits of quantification (LOQs) for SA, AK and SC were 10, 5 and 5 microg/kg, respectively. The average recoveries ranged from 72.1% to 96.8% at the spiked levels of 0.01 and 0.1 mg/L with the relative standard deviations (RSDs) less than 15%. This method is accurate, highly sensitive for qualitative and quantitative analysis of the 3 sweeteners in vinegars.
State Non-Staple Food Quality Supervision and Test Center, Beijing 100070, China. fifayinfeng@yahoo.com.cn
Feng
F
Yin
Zhaowei
Z
Ding
Xue
X
Cao
Jie
J
Gao
Deming
D
Jiang
Denghui
D
Kuang
Yanping
Y
Gu
Guoliang
G
He
D019342:Acetic Acid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003494:Cyclamates / Q000032:analysis; D012439:Saccharin / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013549:Sweetening Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D003494:Cyclamates; D013549:Sweetening Agents; D012439:Saccharin; D019342:Acetic Acid