[In vitro strategies for human gametes production from stem cells].
Embryonic stem cells (ESC) are self-renewal and pluripotent cells that are able to differentiate in vitro into several cell types in favourable conditions. Technical protocols for in vitro gametes production have been developed in mice and human species. The functionality of such differentiated cells is not always analysed and an early meiotic arrest is a current observation. These kinds of experimentations have also been tested from human induced pluripotent stem cells (IPSC). However, differentiation ends shortly at the primordial germ cell stage.
Université Paris-Sud 11, hôpital Paul Brousse, Villejuif, France. lucie.tosca@abc.aphp.fr
Lucie
L
Tosca
Anne-Marie
AM
Courtot
Annelise
A
Bennaceur-Griscelli
Gérard
G
Tachdjian
D000818:Animals; D019070:Cell Lineage; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D060833:Cellular Microenvironment; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis / Q000187:drug effects*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D020554:Sperm Injections, Intracytoplasmic; D013090:Spermatocytes / Q000166:cytology*; D013091:Spermatogenesis / Q000187:drug effects*
D006728:Hormones; D036341:Intercellular Signaling Peptides and Proteins