Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot spring.
An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23(T), was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23(T) is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas chromatography were iso-C(15:0), iso-C(17:0) and iso C(17:1) ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA-DNA hybridization measurements revealed low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23(T) (=DSM 18496(T); LMG 23756(T)).
Unité de Biochimie et Biologie Moléculaire, Université de Tunis El Manar, Campus Universitaire, Tunis, Tunisia. hanenebouraoui@yahoo.fr
Hanene
H
Bouraoui
Melek Ben
MB
Aissa
Feten
F
Abbassi
Jean Pierre
JP
Touzel
Michael
M
O'donohue
Mohamed
M
Manai
D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry; D034301:Deinococcus / Q000145:classification* / Q000302:isolation & purification / Q000528:radiation effects*; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D010802:Phylogeny; D011836:Radiation Tolerance; D014416:Tunisia; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D005227:Fatty Acids