[Quantitative determination of contents of three components in Brucea javanica by HPLC].
OBJECTIVE
To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.


METHODS
The determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.


RESULTS
Linearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.


CONCLUSIONS
The determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.
Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China. zhou110zhong99@sohu.com
Zhongliu
Z
Zhou
Renbing
R
Shi
Bin
B
Liu
Jieming
J
Zou
Lisheng
L
Wang
Jingmin
J
Xia
D032269:Brucea / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification / Q000592:standards; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D011786:Quality Control
D004365:Drugs, Chinese Herbal; D009930:Organic Chemicals