Decreased concentrations of retinol-binding protein 4 in sera of epithelial ovarian cancer patients: a potential biomarker identified by proteomics.
Ovarian cancer is the fifth leading cause of cancer death in women. Absence             of a reliable biomarker precludes early diagnosis of the disease. To identify             new proteins with potential diagnostic or prognostic value for the therapy of             ovarian cancer we performed comparative proteomic analysis of sera from ovarian             cancer patients and healthy women. We analyzed serum samples from 10 patients             diagnosed with epithelial ovarian cancer and 10 age-matched healthy women. To             decrease the extremely wide dynamic range of protein concentrations in serum we             used combinatorial hexapeptide libraries. Serum samples were then subjected to             proteomic 2-DE analysis. Three proteins with differential abundance were found             and identified by mass spectrometry: α-1-antitrypsin, apolipoprotein A-IV and             retinol-binding protein 4. Identification of α-1-antitrypsin and apolipoprotein             A-IV confirms previous studies but the identification of significantly decreased             levels of RBP4 in ovarian cancer patients represents a novel observation. We verified             the decrease of RBP4 levels in ovarian cancer patient sera by two independent             methods and determined absolute RBP4 concentrations in patients and healthy women.             We excluded possible non-cancer factors that could be responsible for the observed             RBP4 decrease. We propose a connection of RBP4 with epithelial ovarian cancer             and advocate the potential of RBP4 as a candidate diagnostic or prognostic biomarker.
Institute of Pathological Physiology, Department of Gynecology and Obstetrics, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Lucie
L
Lorkova
Jana
J
Pospisilova
Jan
J
Lacheta
Sergiu
S
Leahomschi
Jaroslav
J
Zivny
David
D
Cibula
Jan
J
Zivny
Jiri
J
Petrak
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood*; D040901:Proteomics / Q000379:methods; D054839:Retinol-Binding Proteins, Plasma / Q000378:metabolism*
D014408:Biomarkers, Tumor; C101326:RBP4 protein, human; D054839:Retinol-Binding Proteins, Plasma