Phylogenetic diversity of microbial communities associated with the crude-oil, large-insoluble-particle and formation-water components of the reservoir fluid from a non-flooded high-temperature petroleum reservoir.
The diversity of microbial communities associated with non-water-flooded high-temperature reservoir of the Niibori oilfield was characterized. Analysis of saturated hydrocarbons revealed that n-alkanes in crude oil from the reservoir were selectively depleted, suggesting that crude oil might be mildly biodegraded in the reservoir. To examine if any specific microorganism(s) preferentially attached to the crude oil or the other components (large insoluble particles and formation water) of the reservoir fluid, 16S rRNA gene clone libraries were constructed from each component of the reservoir fluid. The clones in the archaeal libraries (414 clones in total) represented 16 phylotypes, many of which were closely related to methanogens. The bacterial libraries (700 clones in total) were composed of 49 phylotypes belonging to one of 16 phylum-level groupings, with Firmicutes containing the greatest diversity of the phylotypes. In the crude-oil- and large-insoluble-particle-associated communities, a Methanosaeta-related phylotype dominated the archaeal sequences, whereas hydrogenotrophic methanogens occupied a major portion of sequences in the library of the formation-water-associated community. The crude-oil associated bacterial community showed the largest diversity, containing 35 phylotypes, 16 of which were not detected in the other bacterial communities. Thus, although the populations associated with the reservoir-fluid components largely shared common phylogenetic context, a specific fraction of microbial species preferentially attached to the crude oil and insoluble particles.
Engineering for Sustainable Carbon Cycle (INPEX Corporation) Social Cooperation Program, Frontier Research Center for Energy and Resources, The University of Tokyo, Eng. Bldg. No. 4, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Hajime
H
Kobayashi
Keita
K
Endo
Susumu
S
Sakata
Daisuke
D
Mayumi
Hideo
H
Kawaguchi
Masayuki
M
Ikarashi
Yoshihiro
Y
Miyagawa
Haruo
H
Maeda
Kozo
K
Sato
D001105:Archaea / Q000145:classification* / Q000235:genetics; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D044822:Biodiversity; D015723:Gene Library; D020459:Genes, rRNA; D006358:Hot Temperature; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D014867:Water