[Relevant aspects of the Hcp100 molecular marker of Histoplasma capsulatum and its potential therapeutic use in histoplasmosis].
BACKGROUND
Fungal pathogens have developed strategies, involving genes expression that favors their persistence and multiplication in the host. The absence of molecules encoded by these genes could interfere with the growth and death of these fungi. In the past, a coactivator protein coding gene (Hcp100) of the fungus Histoplasma capsulatum was reported, which is overexpressed after 1h of contact between fungal yeast-cells and murine macrophages. The product of this gene, a protein of 100 kDa (Hcp100) of H. capsulatum, is probably a regulatory protein involved in the processes required for fungal adaptation and its survival in the intracellular hostile conditions of the macrophages. A 210-bp fragment of the Hcp100 marker has proved to be an excellent tool for H. capsulatum molecular detection in clinical samples. The potential use of this gene as a therapeutic target in Plasmodium falciparum has been explored through the inhibition of both, the gene and the protein p100 of the parasite, by blocking its growth.


METHODS
Based on the above mentioned antecedents, we believe that the Hcp100 has an important role in the development and maintenance of the H. capsulatum yeast cells within macrophages.


CONCLUSIONS
To study the probable function of Hcp100 in the yeast-phase of this fungal pathogen is relevant to understand its activity and to propose it as a therapeutic target for histoplasmosis treatment.
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, México. skapunking 9@yahoo.com.mx
Antonio Ernesto
AE
González-González
Maria Lucia
ML
Taylor
Everardo
E
Curiel-Quesada
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D015415:Biomarkers; D002685:Chiroptera / Q000382:microbiology; D004197:Disease Reservoirs; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000187:drug effects; D006658:Histoplasma / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006660:Histoplasmosis / Q000188:drug therapy* / Q000382:microbiology / Q000662:veterinary; D054884:Host-Pathogen Interactions; D006801:Humans; D008168:Lung / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D058990:Molecular Targeted Therapy; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology / Q000627:therapeutic use; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013329:Structure-Activity Relationship
D000935:Antifungal Agents; D015415:Biomarkers; D005656:Fungal Proteins; C576552:Hcp100 protein, Histoplasma capsulatum; D034741:RNA, Small Interfering