In vitro evaluation of antimicrobial and antioxidant activities of methanolic extract of Jasminum humile leaves.
OBJECTIVE
To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile (J. humile) leaves extract.


METHODS
Methanolic extract of J. humile was evaluated for its antimicrobial activity by using agar well diffusion method & their possible antioxidant assay by two complementary test systems, namely DPPH and hydrogen peroxide scavenging activity. These various antioxidant activities were compared to standard antioxidants such as ascorbic acid for both the tests.


RESULTS
In the DPPH & hydrogen peroxide scavenging activity, the IC(50) value of methanol extract was 70.43 μg/mL & 60.79 μg/mL respectively. Further, the extract showed inhibitory activity for Gram-positive and negative bacteria at different concentrations. The maximum antibacterial activity of extract was exhibited against Staphylococcus aureus (S. aureus) at concentration 50 mg/mL when compared with ciprofloxacin


CONCLUSIONS
These results clearly indicate that J. humile is effective in scavenging free radicals and has the potential to be a powerful antioxidant. Thus, the results obtained in the present study indicate that J. humile leaves extract could be considered as a potential source of natural antioxidants and that could be used as an effective source against bacterial diseases.
MM College of Pharmacy, MM University, Mullana (Ambala)-133207, India. parminder.nain26@gmail.com
Parminder
P
Nain
Ashok
A
Kumar
Sunil
S
Sharma
Jaspreet
J
Nain
D000890:Anti-Infective Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002939:Ciprofloxacin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000037:antagonists & inhibitors; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors; D020128:Inhibitory Concentration 50; D031660:Jasminum / Q000737:chemistry*; D000432:Methanol; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D002939:Ciprofloxacin; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; D000432:Methanol