Effects of ¹⁴C-labelled precursor feeding on production of beauvericin, enniatins H, I, and MK1688 by Fusarium oxysporum KFCC11363P.
The effects of ¹⁴C-labelled precursor feeding on the production of cyclic hexadepsipeptides were investigated by the mycelium of F. oxysporum KFCC11363P producing beauvericin along with enniatins H, I, and MK1688. Most ¹⁴C-phenylalanine and ¹⁴C-valine were incorporated easily into the biosynthetic pathway of ¹⁴C-labelled beauvericin in vivo by the mycelium. However, only a small amount of ¹⁴C-labelled enniatins could be detected by feeding of ¹⁴C-valine. When L-valine was fed as a precursor to the mycelium at large scale, the level of beauvericin increased to maximum followed by enniatins H and I. Feeding of L-valine did not affect the production of enniatin MK1688. These results suggest that L-valine feeding led to the production of D-hydroxyisovaleic acid in the mycelium and specifically enhanced the production of cyclic hexadepsipeptides containing D-hydroxyisovaleic acid, such as beauvericin and enniatins H and I.
Department of Food Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
Hee-Seok
HS
Lee
Kyung-Ai
KA
Kim
Dong-Geun
DG
Seo
Chan
C
Lee
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D047630:Depsipeptides / Q000096:biosynthesis*; D005670:Fusarium / Q000378:metabolism*; D007218:Industrial Microbiology; D015394:Molecular Structure; D025282:Mycelium / Q000378:metabolism; D014633:Valine / Q000737:chemistry*
D002247:Carbon Isotopes; D003470:Culture Media; D047630:Depsipeptides; C543476:MK 1688; C100264:enniatins; C004456:beauvericin; D014633:Valine