Moderate physical training attenuates muscle-specific effects on fibre type composition in adult rats submitted to a perinatal maternal low-protein diet.
OBJECTIVE
To verify whether moderate physical training affects the muscle fibre composition of adult rats subjected to a low protein diet during the perinatal period.


METHODS
Male Wistar rats were divided into two groups according to their mother's diet during gestation and lactation: control (17% casein, C) and low-protein (8% casein, LP). On postnatal day 60, half of each group was submitted to moderate physical training (8 weeks, 5 days/week(-1), 60 min/day(-1), at 70% of VO(2max), T) or not. After the physical training period, soleus and extensor digitorum longus (EDL) muscles were removed. Myofibrillar ATPase staining was used to classify muscle fibres as type I, IIa, IIb, and intermediate.


RESULTS
In the EDL muscle, LP rats showed no changes in the fibre type proportion. Both the C + T and LP + T groups showed a higher percentage of fibres of type IIa, and a lower proportion of fibres of type IIb. In the soleus muscle, LP animals showed a reduction in the proportion of fibre types I and intermediate. C + T rats showed an increase in the fibre type I and IIa. In the LP + T rats, the proportions of the fibre types remained similar to control rats.


CONCLUSIONS
Moderate physical training acts as a positive environmental stimulus that reverts the effects of a perinatal low-protein diet on the proportion of fibre types in skeletal muscle.
Department of Physical Education and Sport Science, Núcleo de Educação Física e Ciências do Esporte, Centro Acadêmico de Vitória CAV, Federal University of Pernambuco, Recife, Brazil. carolleandro22@gmail.com
Carol Góis
CG
Leandro
Wellington
W
da Silva Ribeiro
José Antônio
JA
Dos Santos
Adriano
A
Bento-Santos
Carlos Henrique
CH
Lima-Coelho
Filippe
F
Falcão-Tebas
Cláudia Jacques
CJ
Lagranha
Sandra
S
Lopes-de-Souza
Raul
R
Manhães-de-Castro
Ana Elisa
AE
Toscano
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D018753:Diet, Protein-Restricted; D005260:Female; D008297:Male; D044342:Malnutrition / Q000503:physiopathology; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar