Expression levels of 25 genes in liver and testis located in a QTL region for androstenone on SSC7q1.2.
A quantitative trait locus (QTL) for boar fat androstenone levels has been identified near the SSC7 centromere in a Large White × Meishan cross. Backcrosses were produced to isolate the Chinese haplotype in a European genetic background. The expression of 25 genes from the QTL region was studied in the testes and livers of 5-month-old backcross boars, with the aim of identifying the causal gene. Using Fluidigm, a new high-throughput technology, the expression of 25 genes was measured in a single real-time PCR experiment. This study found six significantly down-regulated genes (C6ORF106, C6ORF81, CLPS, SLC26A8, SRPK1 and MAPK14) in the testes of MS-LW backcross boars. However, according to current knowledge, none of the genes appear to be related to androstenone metabolism. In the livers, none of the genes were significantly up- or down-regulated, including TEAD3, which was previously designated as a possible candidate to explain this QTL.
INRA, UMR444, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France. annie.robic@toulouse.inra.fr
A
A
Robic
K
K
Fève
C
C
Larzul
Y
Y
Billon
M
M
van Son
L
L
Liaubet
J
J
Sarry
D
D
Milan
E
E
Grindflek
J P
JP
Bidanel
J
J
Riquet
D000273:Adipose Tissue / Q000737:chemistry; D000736:Androstenes / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002503:Centromere; D033481:Chromosomes, Mammalian; D015870:Gene Expression; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism*; D008297:Male; D016133:Polymerase Chain Reaction; D040641:Quantitative Trait Loci; D013737:Testis / Q000378:metabolism*
D000736:Androstenes