Protein dynamics and enzymatic chemical barrier passage.
After many decades of investigation, the manner in which enzymes increase the rate of chemical reactions, at times by a factor of 10(17) compared to the rate of the corresponding solution phase reaction, is still opaque. A topic of significant discussion in the literature of the past 5-10 years has been the importance of protein dynamics in this process. This Feature Article will discuss the authors' work on this still controversial topic with focus on both methodology and application to real systems. The end conclusion of this work has been that for specific enzymes under study protein dynamics on both rapid time scales of barrier crossing (termed promoting vibrations by the authors) and of conformational fluctuations are central to the function of biological catalysts. In another enzyme we will discuss, the results are far less clear. The manner of the coupling of chemistry to protein dynamics has deep implications for protein architecture, both natural and created, and recent results reinforce the complexity of the protein form that has evolved to support these functions.
Departments of Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Dimitri
D
Antoniou
Steven D
SD
Schwartz
D055162:Biocatalysis; D020134:Catalytic Domain; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D009010:Monte Carlo Method; D009243:NAD / Q000378:metabolism; D013997:Time Factors
D004798:Enzymes; D009243:NAD; D007770:L-Lactate Dehydrogenase