Preventive effects of supplemental selenium and selenium plus iodine on bone and cartilage development in rats fed with diet from Kashin-Beck disease endemic area.
The purpose of this study was to investigate the effects of supplemental selenium and selenium plus iodine on bone and growth plate cartilage histology and serum biochemistic parameters in rats. Ninety-six Wistar rats were randomly divided into the following four groups: group A, the rats fed with normal diet; group B, fed with diet from Kashin-Beck disease (KBD) endemic area; group C, fed with diet from KBD endemic area supplemented with selenium; and group D, fed with diet from KBD endemic area supplemented with selenium and iodine. After 4, 8, and 12 weeks, bone and cartilage samples were collected from the rats and were examined for morphological changes in the tibial growth zone and for changes in the plate cartilage and metaphysic. Compared to the rats fed with diet from the KBD endemic area, the rats fed with the supplemental selenium or selenium plus iodine exhibited diminished necrosis of the chondrocytes in the growth plate. In the groups of rats receiving supplemental selenium and selenium plus iodine, the bone volume/tissue volume ratio (BV/TV), the trabecular thickness (Tb.Th), and the trabecular number were increased, while the trabecular separation was decreased. In the 12th week of the experiment, BV/TV and Tb.Th were significantly increased in the selenium plus iodine group compared to the selenium group. It is concluded that feeding the diet from the KBD endemic area caused necrosis of chondrocytes and dysfunctions of bone development similar to the pathological changes that are seen in KBD. Selenium and iodine protected chondrocytes in growth plate and promoted the formation of trabecular bone. The effects of selenium plus iodine on bone formation were more obvious than those of selenium alone.
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
Y F
YF
Yao
F X
FX
Pei
X B
XB
Li
J
J
Yang
B
B
Shen
Z K
ZK
Zhou
L
L
Li
P D
PD
Kang
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002356:Cartilage / Q000187:drug effects* / Q000254:growth & development; D004032:Diet; D019587:Dietary Supplements; D004357:Drug Synergism; D019353:Endemic Diseases; D005260:Female; D005269:Femur / Q000187:drug effects / Q000254:growth & development; D006132:Growth Plate / Q000187:drug effects / Q000254:growth & development; D007455:Iodine / Q000008:administration & dosage / Q000494:pharmacology*; D057767:Kashin-Beck Disease / Q000453:epidemiology / Q000517:prevention & control*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D013974:Thyroxine / Q000097:blood; D013977:Tibia / Q000187:drug effects / Q000254:growth & development; D013997:Time Factors; D014284:Triiodothyronine / Q000097:blood; D015430:Weight Gain / Q000187:drug effects
D014284:Triiodothyronine; D007455:Iodine; D012643:Selenium; D013974:Thyroxine