Carbosilane dendrimer 2G-NN16 represses Tc17 differentiation in primary T CD8+ lymphocytes.
We studied changes in gene expression induced by the carbosilane dendrimer 2G-NN16 to evaluate their potential as a vehicle for gene therapy and as medication. Global gene expression profiles on CD8+ T lymphocytes reveal that ribosomal proteins are induced in the presence of 2G-NN16. IL17A and IL17F, the principal interleukins secreted by Tc17 cells, a subset of CD8+ T lymphocytes, were down-regulated when cultured in the presence of this dendrimer. Microarray results were confirmed by real time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). 2G-NN16 also showed a high potential for in vitro inhibition of Tc17 differentiation of CD8+ T lymphocytes in the presence of the Tc17 differentiation molecules IL6 and TGF-B1. These findings suggest that 2G-NN16 could facilitate drug delivery and may be used to treat inflammatory processes driven by Tc17 cells.
Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007-Madrid, Spain.
Rafael
R
Gras
María I
MI
García
Rafael
R
Gómez
F Javier
FJ
de la Mata
M Angeles
MA
Muñoz-Fernández
Luís A
LA
López-Fernández
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D050091:Dendrimers / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D020869:Gene Expression Profiling; D018014:Gene Transfer Techniques / Q000009:adverse effects; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D012821:Silanes / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
C572050:2G-NN16; D000894:Anti-Inflammatory Agents, Non-Steroidal; D050091:Dendrimers; C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D012821:Silanes; C504072:carbosilane