Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women.
The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the α(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.
Department of Sports Medicine, Third Faculty of Medicine, Charles University of Prague, Ruska 87, Prague 10, Czech Republic. katrien.koppo@UGent.be
Katrien
K
Koppo
Michaela
M
Siklová-Vitková
Eva
E
Klimcáková
Jan
J
Polák
Marie A
MA
Marques
Michel
M
Berlan
Johan
J
Van de Voorde
Jens
J
Bulow
Dominique
D
Langin
Isabelle
I
de Glisezinski
Vladimir
V
Stich
D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D031204:Caloric Restriction; D002395:Catecholamines / Q000378:metabolism*; D004038:Diet, Reducing; D005260:Female; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D008066:Lipolysis / Q000502:physiology*; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D015431:Weight Loss / Q000502:physiology*
D002395:Catecholamines; D007328:Insulin