Evaluation of the genotoxic potential of standardized extract of Glycyrrhiza glabra (GutGard™).
Glycyrrhiza glabra Linn. (licorice) is widespread throughout the Mediterranean region and certain areas of Asia. Historically, the dried rhizome and root of the plant were used by the Chinese, Egyptian, Greek, Indian, and Roman civilizations as expectorant and carminative. In the modern medicinal system, licorice is used to treat liver ailments, dyspepsia, bronchitis, rheumatoid arthritis etc. Despite the extensive pharmacological applications, the genotoxic potential of G. glabra extract (GutGard™) has not been evaluated. Hence, this study was conducted to investigate the genotoxic potential of GutGard™ using battery of in vitro test systems: bacterial reverse mutation test (Ames II™), chromosome aberration (CA) and micronucleus (MN) tests. GutGard™ did not show significant increase in number of revertant colonies in Salmonella typhimurium strains (TA98 and TAMix) with/without S9 fraction. In CA and MN studies, GutGard™ did not show clastogenic effect at 4 and 18 h treatments with and without S9 fraction. Results indicated that GutGard™ is not mutagenic in a battery of genotoxicity tests.
R&D Centre, Natural Remedies Pvt. Ltd., Bangalore, India. cvc@naturalremedy.com
C V
CV
Chandrasekaran
K
K
Sundarajan
Anumita
A
Gupta
H S
HS
Srikanth
Jothie
J
Edwin
Amit
A
Agarwal
D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D006035:Glycyrrhiza; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity*; D018517:Plant Roots; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
C554040:GutGard; D010936:Plant Extracts