Adaptation of high-growth influenza H5N1 vaccine virus in Vero cells: implications for pandemic preparedness.
Current egg-based influenza vaccine production technology can't promptly meet the global demand during an influenza pandemic as shown in the 2009 H1N1 pandemic. Moreover, its manufacturing capacity would be vulnerable during pandemics caused by highly pathogenic avian influenza viruses. Therefore, vaccine production using mammalian cell technology is becoming attractive. Current influenza H5N1 vaccine strain (NIBRG-14), a reassortant virus between A/Vietnam/1194/2004 (H5N1) virus and egg-adapted high-growth A/PR/8/1934 virus, could grow efficiently in eggs and MDCK cells but not Vero cells which is the most popular cell line for manufacturing human vaccines. After serial passages and plaque purifications of the NIBRG-14 vaccine virus in Vero cells, one high-growth virus strain (Vero-15) was generated and can grow over 10(8) TCID(50)/ml. In conclusion, one high-growth H5N1 vaccine virus was generated in Vero cells, which can be used to manufacture influenza H5N1 vaccines and prepare reassortant vaccine viruses for other influenza A subtypes.
National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.
Yu-Fen
YF
Tseng
Alan Yung-Chih
AY
Hu
Mei-Liang
ML
Huang
Wei-Zhou
WZ
Yeh
Tsai-Chuan
TC
Weng
Yu-Shuan
YS
Chen
Pele
P
Chong
Min-Shi
MS
Lee
D000220:Adaptation, Biological / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002645:Chickens / Q000821:virology; D002522:Chlorocebus aethiops; D004189:Disaster Planning; D004285:Dogs; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000254:growth & development* / Q000276:immunology*; D007252:Influenza Vaccines / Q000096:biosynthesis* / Q000276:immunology; D007251:Influenza, Human / Q000453:epidemiology* / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D058873:Pandemics / Q000517:prevention & control*; D014709:Vero Cells
D000956:Antigens, Viral; D007252:Influenza Vaccines