[Effect of polypeptide extract from scorpion venom (PESV) on expression of HIF-1alpha and SDF-1/CXCR4 in repopulating H22 tumour tissue during chemotherapy treatment].
OBJECTIVE
To study the expression of HIF-1alpha and SDF-1/CXCR4 in repopulating H22 tumor tissue and the mechanism of angiogenesis of polypeptide extract from scorpion venom (PESV) during chemotherapy treatment.


METHODS
The expression of HIF-1alpha and SDF-1/CXCR4 in H22 tumor tissue was monitored by immunohistochemistry, and the expression level was determined by Qwin V3 image analyzing software. The correlation between HIF-1alpha and SDF-1 was analyzed. SDF-1 content was detected by ELISA.


RESULTS
HIF-1alpha expression was found no difference in model group between 14 d and 21 d, and up-regulated in 28 d. There was no change of HIF-1alpha expression was observed in low-dose PESV group. In high-dose PESV group, the level of HIF-1alpha expression was high in 14 d and low in 21 d. ELISA detecting showed SDF-1 content increased slowly from 14 d to 21 d, highly from 21 d to 28 d. But in high-dose PESV groups, the content increased slowly all the time. The immunohitochemistry method got the same result with ELISA. Correlation analysis showed r = 0.805. CXCR4 expression down-regulated in two PESV treated groups, and no difference was found between these two groups.


CONCLUSIONS
HIF-1alpha and SDF-1 participated in VEGF expression and angiogenesis in tumor tissue during chemotherapy, while PESV could inhibit the expression of HIF-1alpha and SDF-I.
Department of Pathology, Institute of Basic Medicine, Shandong Academy of Medical Science, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China. charle92003@163.com
Zhaopeng
Z
Wang
Weidong
W
Zhang
Licun
L
Wu
Qing
Q
Jia
Zhaoxia
Z
Wang
Yueying
Y
Zhang
Yunna
Y
Ning
D000818:Animals; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D019718:Receptors, CXCR4 / Q000187:drug effects* / Q000378:metabolism; D012604:Scorpion Venoms / Q000737:chemistry / Q000494:pharmacology*; D012605:Scorpions / Q000737:chemistry*; D013997:Time Factors
C514936:CXCR4 protein, mouse; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010455:Peptides; D019718:Receptors, CXCR4; D012604:Scorpion Venoms