Detection, accumulation, distribution, and depletion of furaltadone and nifursol residues in poultry muscle, liver, and gizzard.
Nitrofurans were broadly used as an extremely effective veterinary antibiotic especially in pig and poultry production farms. Because of fears of the carcinogenic effects on humans, the nitrofurans were banned from use in livestock production in many countries, including the European Union. The present study examines the accumulation, distribution, and depletion of furaltadone and nifursol and of their tissue-bound metabolites [3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and 3,5-dinitro-salicylic acid hydrazine (DNSAH), respectively, in poultry edible tissues (muscle, liver, and gizzards) following administration to chickens of therapeutic and subtherapeutic concentrations of both compounds. Nitrofurans determination was performed by high-performance liquid chromatography-diode array detection and liquid chromatography-tandem mass spectrometry, respectively, for feeds and for poultry tissues. Furaltadone and nifursol, in very low concentrations, were found in samples of muscle, liver, and chicken's gizzard collected from slaughtered animals after 5 weeks of treatment and no withdrawal time period. When a withdrawal time period of 3 weeks was respected, no detectable nitrofuran parent compounds was observed in all of the studied matrices. For AMOZ, concentrations of 270 μg/kg in meat, 80 μg/kg in liver, and 331 μg/kg in gizzard were determined after administration of a medicated feed with furaltadone (132 mg/kg), 3 weeks after withdrawal of treatment. For DNSAH, the concentration values obtained are much lower than those observed for AMOZ. For meat, liver, and gizzard, DNSAH concentrations of 2.5, 6.4, and 10.3 μg/kg, respectively, were determined, after administration of a medicated feed with nifursol (98 mg/kg), 3 weeks after withdrawal of treatment. The gizzard could be considered a selected matrix for nitrofuran residues evaluation in poultry, due to its capacity of retaining either nitrofuran parent compounds or metabolites in higher concentrations, regardless of the administered dose or of the respected withdrawal time period.
INRB-LNIV,  Laboratório Nacional de Investigação Veterinária , Estrada de Benfica, 701, 1549-011 Lisboa, Portugal.
Jorge
J
Barbosa
Andreia
A
Freitas
Sara
S
Moura
José Luis
JL
Mourão
Maria Irene
MI
Noronha da Silveira
Fernando
F
Ramos
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis / Q000378:metabolism; D005895:Gizzard, Avian / Q000737:chemistry* / Q000378:metabolism; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008460:Meat / Q000032:analysis; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D009581:Nitrofurans / Q000032:analysis* / Q000378:metabolism; D023303:Oxazolidinones / Q000032:analysis* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D009581:Nitrofurans; D023303:Oxazolidinones; C007795:furaltadon; C032119:nifursol