Coaggregation of amyloid fibrils for the preparation of stable and immobilized enzymes.
Highly stable enzyme coaggregates were developed using amyloid fibrils as support materials. Amyloid fibril formation was induced by ionic liquids, and immobilization was done by the coaggregation of enzymes and amyloid fibrils followed by chemical cross-linking. Transmission and scanning electron microscopy studies were carried out to characterize the coaggregates. The amyloid fibril-linked enzymes showed significantly increased stability against various deactivating conditions. In addition, a high level of reusability was clearly observed. This study clearly demonstrated that amyloid fibrils can be used as biomaterials for enzyme immobilization and that amyloid fibril-linked enzyme coaggregates have good potential for industrial applications.
Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749, South Korea.
Seulgi
S
Kim
Song Yi
SY
Bae
Bun Yeoul
BY
Lee
T Doohun
TD
Kim
D000682:Amyloid / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission
D000682:Amyloid; D004800:Enzymes, Immobilized