Visualization of tyrosinase activity in melanoma cells by a BODIPY-based fluorescent probe.
We have presented a fluorescent probe 1 that exhibits a fluorescence turn-on signal upon reaction with tyrosinase, and we show that it is readily employed for the assessment of tyrosinase activity and tyrosinase inhibitor activity in buffered aqueous solution, and further utilized for the visualization of endogenous tyrosinase activity in living melanoma cells.
Department of Chemistry, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do, 448-701, Korea.
Tae-Il
TI
Kim
Jihye
J
Park
Seonhwa
S
Park
Yongdoo
Y
Choi
Youngmi
Y
Kim
D001896:Boron Compounds / Q000737:chemistry* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008546:Melanoma, Experimental / Q000473:pathology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D014442:Monophenol Monooxygenase / Q000378:metabolism*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D014442:Monophenol Monooxygenase