[Effects of Chinese herbal medicine Yiqi Zengmin formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats].
OBJECTIVE
To investigate the effects of traditional Chinese compound recipe Yiqi Zengmin (YQZM) formula on expression of glucose transporter 4 (GLUT4) in skeletal muscle of rats with type 2 diabetes induced by high-fat diet combined with low-dose-streptozotocin injection.


METHODS
Fifty male rats were randomly divided into two groups: model group fed with high-fat diet (n=40) and control group (n=10). After 4-week feed of high-fat diet, the rats of the model group were injected with streptozotocin at a dose of 35 mg/kg. Rats with plasma glucose over 16.67 mmol/L were randomly divided into 4 groups: diabetic model group, YQZM group, rosiglitazone group and losartan group and were treated with water, YQZM, rosiglitazone or losartan respectively by oral administration for 8 weeks. The expression of GLUT4 protein in the cytoplasm and the plasma membrane of isolated rats' skeletal muscles were detected by Western blotting.


RESULTS
The expression of GLUT4 in the plasma membrane was more than that in the cytoplasm in skeletal muscle tissues of the normal rats, while the expression of GLUT-4 in the plasma membrane was less than that in the cytoplasm in skeletal muscle tissues of the type 2 diabetic rats. YQZM formula enhanced GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, similar to rosiglitazone.


CONCLUSIONS
YQZM formula can enhance GLUT4 translocation from the cytoplasm to the plasma membrane in skeletal muscle tissues, and displays the insulin sensitization characteristic of rosiglitazone.
Institute of Chinese Integrative Medicine, Fudan University, Shanghai, China. honglixue@yahoo.cn
Hong-li
HL
Xue
Zeng
Z
Zhang
Wen-jian
WJ
Wang
Wei-hua
WH
Chen
Jian
J
Ying
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism* / Q000628:therapy; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism* / Q000628:therapy; D059305:Diet, High-Fat; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D051275:Glucose Transporter Type 4; C495694:Slc2a4 protein, rat