A photocurable hydrogel/elastomer composite scaffold with bi-continuous morphology for cell encapsulation.
A photocurable two-phase scaffold with a bi-continuous morphology was designed and characterized for the repair of load bearing soft tissues. An N-methacrylate glycol chitosan (MGC) hydrogel phase was used to distribute the cells and enable cell growth once crosslinked. The second phase, an elastomerprepared from a star-poly(ε-caprolactone-co-D,L-lactide) triacrylate, was used to enhance the mechanical properties. Chondrocytes were photocrosslinked within the bi-continuous scaffolds and proliferated, increased metabolic activity and accumulated extracellular matrix over a 14 d culture period. Also during this time no significant material degradation was observed.
Department of Chemical Engineering, Queen's University, Kingston, K7L 3N6, Canada.
James W S
JW
Hayami
Stephen D
SD
Waldman
Brian G
BG
Amsden
D000818:Animals; D001672:Biocompatible Materials; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D002417:Cattle; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D048271:Chitosan / Q000737:chemistry; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000502:physiology; D020100:Hydrogels; D008689:Methacrylates / Q000737:chemistry; D044367:Phase Transition; D055668:Photochemical Processes; D011091:Polyesters / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014056:Tomography, X-Ray; D014466:Ultraviolet Rays
D001672:Biocompatible Materials; D020100:Hydrogels; D008689:Methacrylates; D011091:Polyesters; C118638:glycol-chitosan; C505727:poly(epsilon-caprolactone-co-lactide); D048271:Chitosan