Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes.
Chemical toxicity of cryoprotectants to in vitro developmental competence of porcine oocytes was examined. In vitro-matured oocytes were exposed to 40 percent ethylene glycol (EG), glycerol (GLY), or 1,2-propanediol (PD), fertilized with spermatozoa, and cultured for 8 days. Compared to treatment with other cryoprotectants, exposure to EG resulted in the development of significantly more blastocysts, but the rate was significantly lower than that of non-exposed control oocytes. In vitro-matured oocytes were also equilibrated in 40 percent EG by 3 multi-step methods, after which their developmental competence was evaluated. The rate of blastocyst development was higher in the 4-step method than in the 2- and 3-step methods of equilibrium. These results indicate that cryoprotectants and equilibration methods affect the developmental competence of porcine oocytes and that EG may be a superior cryoprotectant for vitrification of these cells.
Department of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
M
M
Taniguchi
R
R
Arikawa
Y
Y
Kaedei
F
F
Tanihara
Z
Z
Namula
V L
VL
Viet
Y
Y
Sato
T
T
Otoi
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D047108:Embryonic Development / Q000187:drug effects*; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019946:Propylene Glycol / Q000494:pharmacology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D013552:Swine
D003451:Cryoprotective Agents; D019946:Propylene Glycol; D019855:Ethylene Glycol; D005990:Glycerol