Comparing the tolerance limits of selected bacterial and protozoan species to nickel in wastewater systems.
Heavy-metal resistant microorganisms play a significant role in the treatment of industrial wastewater. The detoxifying ability of these resistant microorganisms can be manipulated for bioremediation of heavy metals in wastewater systems. This study aimed at comparing the tolerance limit of selected wastewater protozoan species (Aspidisca sp., Trachelophyllum sp. and Peranema sp.) against Ni(2+) with that of selected bacterial species (Bacillus licheniformis-ATCC12759, Brevibacillus laterosporus-ATCC64 and Pseudomonas putida-ATCC31483) commonly found in wastewater systems. The isolates were exposed to various concentrations of Ni(2+) in mixed liquor and their tolerance to Ni(2+) assessed at different temperatures (25°C, 30°C, 35°C and 40°C) and pHs (4, 6, 7, 8 and 10). The physicochemical parameters such as chemical oxygen demand (COD) and dissolved oxygen (DO) of the media and the growth rates of the isolates were measured using standard methods. In terms of their minimum inhibitory concentrations (MIC), the results revealed that the isolates could tolerate Ni(2+) at concentrations ranging between 32 and 52ppm for protozoa and between 52 and 84ppm for bacteria. B. licheniformis-ATCC12759 was the most tolerant bacterial species (MIC: 84ppm-Ni(2+)) while Peranema sp. was the most tolerant protozoan species (MIC: 52ppm-Ni(2+)). At 10 and/or 20ppm-Ni(2+) the growth of B. licheniformis-ATCC12759 (6.30 days(-1) for 10 and 5.73 days(-1) for 20ppm-Ni(2+)), P. putida-ATCC31483 (6.02 days(-1) for 10 and 5.31 days(-1) for 20ppm-Ni(2+)) and Peranema sp. (2.15 days(-1) for 10ppm-Ni(2+)) was stimulated after one day of incubation. Statistical evidence showed significant differences (p=0.0065) between the MIC of the six isolates and positive correlations between COD and the growth rates of isolates (r=0.8999/0.8810 for bacteria/protozoa). The tolerance limit of all isolates was significantly dependent on the pH and the temperature. The study suggests that these isolates can be used for the bioremediation of nickel in industrial wastewater systems.
Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Arcadia Campus, P/Bag X680, Pretoria 0001, South Africa.
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Kamika
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Momba
D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D016798:Ciliophora / Q000187:drug effects* / Q000254:growth & development; D016822:Euglenida / Q000187:drug effects* / Q000254:growth & development; D009532:Nickel / Q000494:pharmacology*; D012621:Seasons; D013019:South Africa; D014865:Waste Disposal, Fluid / Q000379:methods*
D009532:Nickel