Changes of renal vitamin D metabolic enzyme expression and calcium transporter abundance in obstructive nephropathy.
The effects of urinary-tract obstruction on renal function have been clarified. However, there is little known about the change of renal vitamin D metabolic enzyme expression and vitamin D-dependent calcium transporting proteins expression in obstructive nephropathy. The male mice were subjected to unilateral ureteral obstruction (n = 10) or sham operation (n = 10). All mice were killed on day 7 after the surgical operation. Kidney sections were stained with Masson's trichrome and gene expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. The obstructed kidney exhibited interstitial fibrosis as shown by the strong collagen deposition in the interstitium. Quantitative PCR results showed the increase of 1-OHase (P < 0.001) mRNA expression and the decrease of 24-OHase (P < 0.01), CaBP-9k (P < 0.01) and CaBP-28k (P < 0.01) mRNA expression in obstructed kidney as compared to that of the Sham group. In addition, the mRNA expression of 1-OHase and CaBP-9k was significantly increased and decreased, respectively, in obstructed kidney as compared to that of the contra-lateral kidney in unilateral ureteral obstruction (UUO) mice. Together, the present finding supports the hypothesis that the ureteral obstruction leads to the alteration of renal vitamin D metabolic enzyme expression and calcium transporter abundance, which may secondarily induce the abnormality of vitamin D endocrine system and bone health.
Center of System Biomedical Sciences, University of Shanghai for Science and Technology, Shanghai, China. medicineyan@yahoo.com.cn
Yan
Y
Zhang
Shu-Yan
SY
Wu
Sa-Sa
SS
Gu
Fu-Kou
FK
Lv
D000818:Animals; D064026:Calbindins; D002118:Calcium / Q000097:blood; D015220:Calcium Channels / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013255:Steroid 21-Hydroxylase / Q000378:metabolism*; D013250:Steroid Hydroxylases / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000378:metabolism; D014517:Ureteral Obstruction / Q000378:metabolism* / Q000503:physiopathology; D014807:Vitamin D / Q000378:metabolism*; D065668:Vitamin D3 24-Hydroxylase
D064026:Calbindins; D015220:Calcium Channels; D064030:S100 Calcium Binding Protein G; D050916:TRPV Cation Channels; C492240:Trpv5 protein, mouse; D014807:Vitamin D; D013250:Steroid Hydroxylases; C531511:Cyp21a1 protein, mouse; D013255:Steroid 21-Hydroxylase; D065668:Vitamin D3 24-Hydroxylase; D000252:Calcium-Transporting ATPases; D002118:Calcium