Oxidative DNA damage estimated by urinary 8-hydroxy-2'-deoxyguanosine and arsenic in glass production workers.
A total of 130 male glass workers, including 33 administrative workers, 18 batch house workers, 42 craftsmen, and 37 melting process workers, were recruited to investigate the potential DNA damage resulting from toxic element exposure. The occupational exposure to trace elements, including arsenic (As), cadmium (Cd), manganese (Mn), nickel (Ni), lead (Pb), and selenium (Se), was estimated by their urinary levels as internal doses. In addition, all participants filled a self-filled questionnaire indicating their individual information. The average levels of urinary As, Cd, Mn, Ni, Pb, Se, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were 282.3 ± 464.6, 3.07 ± 5.39, 3.81 ± 11.43, 81.48 ± 138.9, 18.23 ± 49.61, 165.2 ± 224.9, and 17.21 ± 26.34 μg/g creatinine, respectively. The urinary levels of 8-OHdG and toxic elements were strongly associated with the work nature of the worker, with an exception of Mn and Pb. In contrast, the levels of toxic element were not influenced by age, smoking behavior, and alcohol consumption. The urinary 8-OHdG was found significantly higher in higher internal exposure groups of As, Cd, Ni, and Se. However, the stepwise multiple regression models showed that urinary 8-OHdG was only associated with urinary As and heat stress but inversely with age.
Department of Safety, Health, and Environmental Engineering, National United University, Miaoli, Taiwan. tslin@nuu.edu.tw
Tser-Sheng
TS
Lin
Chin-Ching
CC
Wu
Jyun-De
JD
Wu
Chun-Han
CH
Wei
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000704:Analysis of Variance; D001151:Arsenic / Q000652:urine*; D020261:Arsenic Poisoning / Q000209:etiology / Q000652:urine*; D015415:Biomarkers / Q000652:urine; D003404:Creatinine / Q000652:urine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005898:Glass; D006358:Hot Temperature; D006801:Humans; D007221:Industry; D008297:Male; D019216:Metals, Heavy / Q000633:toxicity / Q000652:urine; D008875:Middle Aged; D009622:Noise; D009784:Occupational Diseases / Q000209:etiology / Q000652:urine*; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D012044:Regression Analysis; D011795:Surveys and Questionnaires
D015415:Biomarkers; D019216:Metals, Heavy; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine; D001151:Arsenic