Radio-attenuated leishmanial parasites as immunoprophylactic agent against experimental murine visceral leishmaniasis.
The present study intends to evaluate the role of radio-attenuated leishmania parasites as immunoprophylactic agents for experimental murine visceral leishmaniasis. BALB/c mice were immunized with gamma (γ)-irradiated Leishmania donovani. A second immunization was given after 15 days of first immunization. After two immunizations, mice were infected with virulent L. donovani promastigotes. Protection against Kala-azar (KA) was estimated from spleen and liver parasitic burden along with the measurement of nitrite and superoxide anion generation by isolation of splenocytes and also by T-lymphocyte helper 1(Th1) and T-lymphocyte helper 2(Th2) cytokines release from the experimental groups. It was observed that BALB/c mice having prior immunization with radio-attenuated parasites showed protection against L. donovani infection through higher expression of Th1 cytokines and suppression of Th2 cytokines along with the generation of protective free radicals. The group of mice without prior priming with radio-attenuated parasites surrendered to the disease. Thus it can be concluded that radio-attenuated L. donovani may be used for.
Department of Zoology, Bethune College, 181 Bidhan Sarani, Kolkata 700 006, India.
Sanchita
S
Datta
Rupchand
R
Adak
Priyanka
P
Chakraborty
Arun Kumar
AK
Haldar
Surajit
S
Bhattacharjee
Anindita
A
Chakraborty
Syamal
S
Roy
Madhumita
M
Manna
D000818:Animals; D006224:Cricetinae; D016207:Cytokines / Q000032:analysis; D005260:Female; D005720:Gamma Rays; D006801:Humans; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology* / Q000528:radiation effects*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009573:Nitrites / Q000378:metabolism; D016052:Protozoan Vaccines; D013154:Spleen / Q000469:parasitology / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D014613:Vaccines, Attenuated
D016207:Cytokines; D009573:Nitrites; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated; D013481:Superoxides