Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events.
Boron-doped diamond (BDD) interfaces were chemically functionalized through the catalyst free thiol-yne reaction. Different thiolated precursors (e.g., perfluorodecanethiol, 6-(ferrocenyl)-hexanethiol, DNA) were successfully "clicked" to alkynyl-terminated BDD by irradiating the interface at 365 nm for 30 min. Thiolated oligonucleotide strands were immobilized using the optimized reaction conditions, and the surface concentration was tuned to obtain a surface coverage of 3.1 × 10(12) molecules cm(-2). Electrochemical impedance spectroscopy (EIS) was employed to follow the kinetics of hybridization and dehybridization events. The sensitivity of the oligonucleotide modified BDD interface was assayed, and a detection limit of 1 nM was obtained.
Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université de Lille1, Parc de la Haute Borne, 50 avenue de Halley, B;P 70478, 59658 Villeneuve d'Ascq Cedex, France.
Dalila
D
Meziane
Alexandre
A
Barras
Alexander
A
Kromka
Jana
J
Houdkova
Rabah
R
Boukherroub
Sabine
S
Szunerits
D001895:Boron / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D018130:Diamond; D058266:Dielectric Spectroscopy; D004566:Electrodes; D009693:Nucleic Acid Hybridization; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D013438:Sulfhydryl Compounds; D018130:Diamond; D004247:DNA; D001895:Boron