Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA.
Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification.
Translational Genomics Research Institute, Flagstaff, Arizona, United States of America. dengelthaler@tgen.org
David M
DM
Engelthaler
Jolene
J
Bowers
James A
JA
Schupp
Talima
T
Pearson
Jennifer
J
Ginther
Heidie M
HM
Hornstra
Julia
J
Dale
Tasha
T
Stewart
Rebecca
R
Sunenshine
Victor
V
Waddell
Craig
C
Levy
John
J
Gillece
Lance B
LB
Price
Tania
T
Contente
Stephen M
SM
Beckstrom-Sternberg
David D
DD
Blaney
David M
DM
Wagner
Mark
M
Mayo
Bart J
BJ
Currie
Paul
P
Keim
Apichai
A
Tuanyok
D001130:Arizona / Q000453:epidemiology; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D005838:Genotype; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis* / Q000382:microbiology*; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010802:Phylogeny