Evaluation method for gene transfection by using the period of onset of gene expression and cell division.
Using single-cells time-lapse analysis, we investigated the mechanism of gene expression using nine transfection reagents. Although onset of gene expression occurred after cell division by all reagents, 91.6% periods, which depended on onset and cell division, had statistical significance. Evaluation of those periods is useful for elucidating mechanism of transfection.
Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, 1–3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Kazumi
K
Hakamada
Jun
J
Miyake
D002455:Cell Division; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D059010:Single-Cell Analysis; D013997:Time Factors; D014162:Transfection / Q000379:methods*