Analysis of drugs of abuse in hair: evaluation of the immunochemical method VMA-T vs. LC-MS/MS or GC-MS.
Hair analysis is an elaborate and time-consuming multi-step process. The immunometric test VMA-T from Comedical has been evaluated as screening assay for hair analysis. From routine work, authentic samples were selected that were positive for opiates, cocaine, MDMA-type drugs, amphetamines, methadone or THC. These hair samples were investigated by LC-MS or GC-MS and the VMA-T procedure, respectively. Using the cut-off values recommended by the Society of Hair Testing, the VMA-T method discriminates with good sensitivity between negative and positive hair samples and is an expedient screening method for drugs in keratinized matrices such as hair. In order to save time and resources, the residue of the VMA-T extraction solution can be reused for confirmation analysis by LC-MS except for cocaine.
Institute of Legal Medicine, University of Zurich, 8006 Zurich, Switzerland. markus.baumgartner@irm.uzh.ch
Markus R
MR
Baumgartner
Rosetta
R
Guglielmello
Monique
M
Fanger
Thomas
T
Kraemer
D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D009294:Narcotics / Q000032:analysis*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
D009294:Narcotics