[Cutting propagation of Periploca forrestii and dynamic analyses of physiological and biochemical characteristitics related to adventitious roots formation].
OBJECTIVE
To study the effects of different auxcin regulators on rooting of Periplocaforrestii cuttings as well as dynamic change rules of endogenous plant hormones and oxidases related to adventitious root formation.


METHODS
Cuttings propagation characters of Periploca forrestii were investigated and compared with different concentration treatments of indolebutyric acid (IBA), Rooting Powder No. 1 (ABT1) and naphthylacetic acid (NAA). The dynamic changes of contents of endogenous hormones including indole acetic acid (IAA), abscisic acid (ABA), zeatin ribosides (ZRs) as well as the activities of indoleacetic acid oxidase( IAAO), polyphenol oxidase (PPO), peroxidase (POD) were tested.


RESULTS
Rooting percentage of cutting with 150 mg/L IBA, 150 mg/L ABT1 treatment and NAA treatment were 80% ,70% and 68% respectively, rooting percentage of cuttings of the control was 23% only. The adventitious rooting displayed three distinct phases i. e. root-inducing, root-formating and root-elongating phases. During root-inducing phase the contents of IAA, ABA and ZRs decreased,whereas IAAO activity kept at a higher level. The IAA content reached the peak and PPO activity increased obviously during root-formating phase, while activities of IAAO, POD and contents of ABA, ZRs declined to minimum. During root-elongating phase contents of IAA, ABA, ZRs were much steadfast and activities of PPO, IAAO, POD were increased. After that, the activities of the three oxidases decreased slowly. 150 mg/L IBA treatment increased the content of IAA and PPO activity in cuttings during rooting,while the opposite result occurred in contents of ZRs, ABA and the activities of IAAO, POD.


CONCLUSIONS
The dynamic changes of endogenous hormones (IAA, ABA, ZRs) contents and IAAO, PPO, POD activities are tightly related to the rooting process of cuttings in Periploca forrestii.
College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China. gaojiexn@sohu.com
Jie
J
Gao
Xiao-fei
XF
Zeng
Xiao-hui
XH
Liu
Shang-xing
SX
Yang
D000040:Abscisic Acid / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D046508:Culture Techniques; D007210:Indoleacetic Acids / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D009280:Naphthaleneacetic Acids / Q000378:metabolism / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D031109:Periploca / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology*
D007210:Indoleacetic Acids; D007211:Indoles; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; C014612:indolebutyric acid; C030737:indoleacetic acid; D000040:Abscisic Acid; D010088:Oxidoreductases; D004156:Catechol Oxidase; C027929:indoleacetic acid oxidase; D010544:Peroxidases