Multilayer chitosan-based open tubular capillary anion exchange column with integrated monolithic capillary suppressor.
We describe a multilayered open tubular anion exchange column fabricated by alternately pumping solutions of chitosan and glutaraldehyde. The column is terminated in an integrally bonded monolithic suppressor cast around a mandrel of a tungsten wire, composed of an acrylic acid (AA)-ethylene dimethacrylate (EDMA) monolith that is made with sufficient porogen for the monolith to function as a membrane. For a 4.5m long 75 μm bore column coated with 24 successive layers of the condensation polymer (estimated to contain ~72 molecular layers) and coupled to 1cm length of a suppressor fabricated with 55-60% AA, effective separation of several common anions (F(-), Cl(-), NO(2)(-), Br(-), NO(3)(-), average number of theoretical plates ~12,000) and adequate suppression of 1 mM KOH used as eluent was observed at a flow rate of 800 nL min(-1) to obtain sub-picomol detection limits at an operating pressure of ~1 bar. The separation is not time efficient but the system can be meritorious in unique niche applications where a small form factor is desired and liquid volume and power consumption are more important than separation speed.
State Key Laboratory of Marine Environmental Science, Environmental Science Research Center, Xiamen University, Xiamen 361005, China.
Xiaojia
X
Huang
Frank W
FW
Foss
Purnendu K
PK
Dasgupta
D053801:Capillary Electrochromatography / Q000379:methods*; D048271:Chitosan / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006898:Hydroxylamines / Q000032:analysis / Q000737:chemistry; D008689:Methacrylates / Q000032:analysis / Q000737:chemistry
D006898:Hydroxylamines; D008689:Methacrylates; C494687:N,O-dimethacryloylhydroxylamine; D048271:Chitosan