title: Use of mutagenically separated PCR for the detection of the mutation associated with porcine stress syndrome.
abstract: A point mutation in the Ryr1 gene encoding the ryanodine receptor in porcine skeletal muscle is associated with enhanced growth characteristics and leanness but also with porcine stress syndrome and pale, soft exudative meat in some animals. The current diagnostic test for the mutation is based on the polymerase chain reaction (PCR), followed by a restriction enzyme digestion step, prior to agarose gel electrophoresis. Using a technique known as mutagenically separated PCR (MS-PCR), a one-step procedure for the identification of the point mutation associated with porcine stress syndrome has been developed. This removes the requirement of the current PCR-based test for restriction enzyme digestion, is consequently quicker to perform, and may lend itself more readily to automation. DNA from blood samples from a series of animals were genotyped using both the conventional test and MS-PCR, and complete agreement between the two methods was obtained.
authors: Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
authors: A K
authors: AK
authors: Lockley
authors: J S
authors: JS
authors: Bruce
authors: S J
authors: SJ
authors: Franklin
authors: R G
authors: RG
authors: Bardsley