title: Prorenin receptor is essential for normal podocyte structure and function.
abstract: The prorenin receptor is an accessory subunit of the vacuolar H(+)-ATPase, suggesting that it has fundamental functions beyond activation of the local renin-angiotensin system. Podocytes express the prorenin receptor, but its function in these cells is unknown. Here, podocyte-specific, conditional, prorenin receptor-knockout mice died of kidney failure and severe proteinuria within 4 weeks of birth. The podocytes of these mice exhibited foot process effacement with reduced and altered localization of the slit-diaphragm proteins nephrin and podocin. Furthermore, the podocytes contained numerous autophagic vacuoles, confirmed by enhanced accumulation of microtubule-associated protein 1 light chain 3-positive intracellular vesicles. Ablation of the prorenin receptor selectively suppressed expression of the V(0) c-subunit of the vacuolar H(+)-ATPase in podocytes, resulting in deacidification of intracellular vesicles. In conclusion, the prorenin receptor is important for the maintenance of normal podocyte structure and function.
authors: Department of Endocrinology & Anti-Aging Medicine and Internal Medicine, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo, 160-8582, Japan.
authors: Yoichi
authors: Y
authors: Oshima
authors: Kenichiro
authors: K
authors: Kinouchi
authors: Atsuhiro
authors: A
authors: Ichihara
authors: Mariyo
authors: M
authors: Sakoda
authors: Asako
authors: A
authors: Kurauchi-Mito
authors: Kanako
authors: K
authors: Bokuda
authors: Tatsuya
authors: T
authors: Narita
authors: Hideaki
authors: H
authors: Kurosawa
authors: Ge-Hong
authors: GH
authors: Sun-Wada
authors: Yoh
authors: Y
authors: Wada
authors: Taketo
authors: T
authors: Yamada
authors: Minoru
authors: M
authors: Takemoto
authors: Moin A
authors: MA
authors: Saleem
authors: Susan E
authors: SE
authors: Quaggin
authors: Hiroshi
authors: H
authors: Itoh
mesh_terms: D000818:Animals; D016923:Cell Death; D051379:Mice; D050199:Podocytes / Q000502:physiology* / Q000648:ultrastructure*; D011956:Receptors, Cell Surface / Q000502:physiology*; D000093342:Prorenin Receptor
chemical_list: D011956:Receptors, Cell Surface; D000093342:Prorenin Receptor