title: Transferrin-conjugated curcumin-loaded superparamagnetic iron oxide nanoparticles induce augmented cellular uptake and apoptosis in K562 cells.
abstract: Superparamagnetic iron oxide nanoparticles are currently used for precise drug delivery and as an image contrast agent. In the present study, the potentiality of curcumin-loaded magnetic nanoparticles (Cur-MNPs) for the treatment of chronic myeloid leukemia (CML) was investigated. For active therapy, transferrin (Tf) ligand was further conjugated to Cur-MNPs, which demonstrated enhanced uptake compared to Cur-MNPs in p210bcr/abl-positive cell line (K562). Cur-MNPs demonstrated greater and sustained anti-proliferative activity in a dose- and time-dependent manner; however, with the advent of a magnetic field the anti-proliferative activity of Cur-MNPs as well as Tf-Cur-MNPs was enhanced due to higher cellular uptake with enhanced cytotoxicity activity. Down-regulation of Bcr-Abl protein activates intrinsic apoptotic pathways for promoting anti-leukemic responses. Our in vitro results advocate potential clinical applications of Cur-MNPs by activating multiple signaling pathways for provoking the anti-leukemic activity.
authors: Laboratory of Nanomedicine, Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar 751023, Orissa, India.
authors: Fahima
authors: F
authors: Dilnawaz
authors: Abhalaxmi
authors: A
authors: Singh
authors: Sanjeeb Kumar
authors: SK
authors: Sahoo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D003911:Dextrans / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D008027:Light; D060328:Magnetic Phenomena; D009682:Magnetic Resonance Spectroscopy; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D060888:Real-Time Polymerase Chain Reaction; D012542:Scattering, Radiation; D015398:Signal Transduction / Q000187:drug effects; D014168:Transferrin / Q000378:metabolism*
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D014168:Transferrin; C065507:ferumoxides; D003474:Curcumin