title: Protective effects of zinc, pentoxifylline, and N-acetylcysteine in an animal model of laparoscopy-induced ischemia/reperfusion injury of the small intestine.
abstract: BACKGROUND
  Pneumoperitoneum induced to facilitate laparoscopy is associated with splanchnic hypoperfusion, ischemia/reperfusion (I/R) injury, and oxidative stress.
  
  
  OBJECTIVE
  This study investigated the effects of pre- and postlaparoscopic conditioning, zinc, pentoxifylline, and N-acetylcysteine on markers of I/R injury of the small intestine in an animal model.
  
  
  METHODS
  Male Sprague-Dawley rats (n=56) were randomized to 1 of 7 treatment groups. Except for group 7 (control group that underwent sham surgery without pneumoperitoneum), pneumoperitoneum was induced in all by use of carbondioxide insufflation under a pressure of 15 mmHg. Group 1 (laparoscopic I/R) was subjected to 60 minutes of pneumoperitoneum. Group 2 (laparoscopic preconditioning plus laparoscopy) was subjected to 5 minutes of insufflation and 5 minutes of desufflation followed by 60 minutes of pneumoperitoneum. Group 3 (laparoscopy plus laparoscopic postconditioning) was subjected to 60 minutes of pneumoperitoneum and 60 minutes of desufflation followed by 5 minutes of insufflation and 5 minutes of desufflation. Group 4 (zinc), group 5 (pentoxifylline), and group 6 (N-acetylcysteine) received a single intraperitoneal injection of zinc (50 mg/kg), pentoxifylline (50 mg/kg), or N-acetylcysteine (150 mg/kg), respectively, 5 minutes before the desufflation period. Animals were killed at the end of the experiments, and small intestine samples were tested for malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD).
  
  
  RESULTS
  There was no significant difference for MDA levels between each other in the groups 1, 2, and 3. But MDA levels were higher significantly in groups 1, 2, and 3 than those of groups 4, 5, 6, and 7. SOD and GPX levels of group 3 were significantly higher than those of groups 1, 2, 4 through 7. According to the SOD levels, there was no significant difference between each other in those of groups 1, 2, 4 through 7. GPX levels of group 3 were significantly higher than those of groups 1, 2, 4 through 7. GPX levels of group 1 were significantly lower than those of groups 5 and 7. The mean CAT level of groups 1 and 2 was significantly lower than that of groups 3, 6, and 7. The mean CAT level of group 3 was significantly higher than that of groups 1, 2, 4 through 7.
  
  
  CONCLUSIONS
  In this animal model of small intestine I/R injury, laparoscopy caused small intestine ischemia as evidenced by elevated markers of tissue I/R injury. This effect was significantly attenuated by zinc, pentoxifylline, and N-acetylcysteine, but not by prelaparoscopy conditioning and postlaparoscopy conditioning.
authors: Department of General Surgery, Kahramanmaras Sutcu Imam University, Faculty of Medicine, Kahramanmaras, Turkey. Ertanbulbuloglu@yahoo.com.tr
authors: Ertan
authors: E
authors: Bulbuloglu
authors: Huseyin
authors: H
authors: Yildiz
authors: Nimet
authors: N
authors: Senoglu
authors: Ismail
authors: I
authors: Coskuner
authors: M Fatih
authors: MF
authors: Yuzbasioglu
authors: Metin
authors: M
authors: Kilinc
authors: Zafer
authors: Z
authors: Dogan
authors: Caglayan
authors: C
authors: Deniz
authors: Hafize
authors: H
authors: Oksuz
authors: Bulent
authors: B
authors: Kantarçeken
authors: Yalcın
authors: Y
authors: Atli
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007421:Intestine, Small / Q000098:blood supply*; D010535:Laparoscopy / Q000009:adverse effects*; D008297:Male; D010431:Pentoxifylline / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology* / Q000517:prevention & control*; D015032:Zinc / Q000627:therapeutic use*
chemical_list: D016166:Free Radical Scavengers; D015032:Zinc; D010431:Pentoxifylline; D000111:Acetylcysteine