title: [Analysis of antibiotic diffusion from agarose gel by spectrophotometry and laser interferometry methods].
abstract: The aim of this study was to analysis of antibiotics (ampicilin, streptomycin, ciprofloxacin or colistin) release from agarose gel by spectrophotmetry and laser interferometry methods. The interferometric system consisted of a Mach-Zehnder interferometer with a He-Ne laser, TV-CCD camera, computerised data acquisition system and a gel system. The gel system under study consists of two cuvettes. We filled the lower cuvette with an aqueous 1% agarose solution with the antibiotics at initial concentration of antibiotics in the range of 0.12-2 mg/ml for spectrophotmetry analysis or 0.05-0.5 mg/ml for laser interferometry methods, while in the upper cuvette there was pure water. The diffusion was analysed from 120 to 2400 s with a time interval of deltat = 120 s by both methods. We observed that 0.25-1 mg/ml and 0,05 mg/ml are minimal initial concentrations detected by spectrophotometric and laser interferometry methods, respectively. Additionally, we observed differences in kinetic of antibiotic diffusion from gel measured by both methods. In conclusion, the laser interferometric method is a useful tool for studies of antibiotic release from agarose gel, especially for substances are not fully soluble in water, for example: colistin.
authors: Zakład Mikrobiologii, Uniwersytet im. Jana Kochanowskiego, Kielce. arabski@ujk.edu.pl
authors: Michał
authors: M
authors: Arabski
authors: Sławomir
authors: S
authors: Wasik
authors: Patrycja
authors: P
authors: Piskulak
authors: Natalia
authors: N
authors: Góźdź
authors: Andrzej
authors: A
authors: Slezak
authors: Wiesław
authors: W
authors: Kaca
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D004058:Diffusion; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D012685:Sepharose / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D012685:Sepharose