title: Controversial behavior of aminoguanidine in the presence of either reducing sugars or soluble glycated bovine serum albumin.
abstract: The elucidation of the controversial inhibitory effect of aminoguanidine (AG) on the cross-linking and fluorescent advanced glycation end products (AGEs) formation during long-term in vitro glycation of type I collagen with 250 mM reducing sugars or 0.5mg/ml soluble glycated bovine serum albumin (AGE-BSA) was researched. Chromatographic and SDS-PAGE analyses revealed the formation of aggregates during collagen glycation. AG at all concentrations (5-80 mM) prevented the cross-linking of collagen peptides with monosaccharides but an increase in fluorescence with a maximum value at 10 mM AG was noticed. In the presence of AGE-BSA, AG prevented the cross-linking process and decreased the fluorescence levels in a concentration-dependent manner. Our results suggest that AG is an efficient inhibitor of collagen cross-linking and the highest increase in fluorescence due to reducing sugars and AG can be explained by the competition between guanidine group of AG and arginine residues of some protein-bound dideoxyosones, which could form fluorescent compounds.
authors: University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 105 Splaiul Independentei 050097, Bucharest 5, Romania. irensro@yahoo.com
authors: Andreea Iren
authors: AI
authors: Serban
authors: Marieta
authors: M
authors: Costache
authors: Anca
authors: A
authors: Dinischiotu
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D024042:Collagen Type I / Q000037:antagonists & inhibitors; D017127:Glycation End Products, Advanced / Q000138:chemical synthesis / Q000737:chemistry; D006146:Guanidines / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility
chemical_list: D002241:Carbohydrates; D024042:Collagen Type I; D017127:Glycation End Products, Advanced; D006146:Guanidines; D012710:Serum Albumin, Bovine; C004479:pimagedine