title: The effect of proteasome on myofibrillar structures in bovine skeletal muscle.
abstract: When bovine myofibrils are incubated with the 20S proteasome their structure is rapidly damaged with loss of material, particularly from the Z discs and I bands. After 24 hr of incubation the myofibrils rupture and debris appears. Certain myofibrillar proteins, including nebulin, myosin, actin and tropomyosin, are hydrolysed during the incubation; others are solubilised (α-actinin). The 20S proteasome completely and rapidly hydrolyses purified myofibrillar proteins in an energy-independent manner. This shows that the 20S proteasome probably plays a role in the postmortem transformation of muscle and more generally in the hydrolysis of cellular proteins.(1).
authors: Université Blaise Pascal, Clermont II, Laboratoire de Biochimie Appliquée, 63177 Aubiere Cedex, France.
authors: N
authors: N
authors: Robert
authors: M
authors: M
authors: Briand
authors: R
authors: R
authors: Taylor
authors: Y
authors: Y
authors: Briand