title: Association between Th-17 cytokine profile and clinical features in patients with spondyloarthritis.
abstract: OBJECTIVE
  In recent years, a substantial amount of information has become available on the relationship between cytokines associated with the Th-17 profile and the development of spondyloarthritis (SpA). The purpose of this study was to evaluate inflammation markers in serum and synovial fluid (SF) and levels of cytokines related to the Th-17 profile in patients with different subtypes of SpA and healthy subjects.
  
  
  METHODS
  We evaluated this cytokine profile in light of the clinical activity of the disease in 62 patients. Serum cytokine levels (IL-17, IL-6, IL-1 alpha, TNF alpha, IFN-gamma) were measured by flow cytometry. IL-23, serum amyloid (SAA) and metalloproteinase 3 (MMP-3) were measured with ELISA. In all patients, clinical evaluation was performed using the activity and function indexes of the disease.
  
  
  RESULTS
  A comparison showed that IL-17, IL-23, IL-1 alpha, IL-6, and TNF-alpha levels were significantly higher in the serum of SpA patients than healthy subjects (HS), and there were no differences among SpA subtypes. In SF we found higher concentrations of cytokines, but only IL-23 showed significant differences (p<0.05). We found a relationship between enthesitis and peripheral involvement and serum IL-17 levels (9 to 63 pg / ml). There was a correlation between levels above 63 pg/ml and a history of infection. Higher levels of IL-23 in synovial fluid could suggest local amplification of the Th-17 cytokine profile.
  
  
  CONCLUSIONS
  These results suggest a possible relationship between IL-17 and enthesis involvement in SpA.
authors: Spondyloarthropathy Group, Division of Rheumatology, Hospital Militar Central/Universidad de la Sabana, Bogota', Colombia. spacolombia@hotmail.com
authors: C
authors: C
authors: Romero-Sanchez
authors: Diego A
authors: DA
authors: Jaimes
authors: J
authors: J
authors: Londoño
authors: J
authors: J
authors: De Avila
authors: Jaime E
authors: JE
authors: Castellanos
authors: Juan M
authors: JM
authors: Bello
authors: W
authors: W
authors: Bautista
authors: R
authors: R
authors: Valle-Oñate
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D053759:Interleukin-23 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood; D000685:Serum Amyloid A Protein / Q000378:metabolism; D025241:Spondylarthritis / Q000276:immunology* / Q000378:metabolism*; D013582:Synovial Fluid / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; C508600:IL6 protein, human; D020381:Interleukin-17; D053759:Interleukin-23; D015850:Interleukin-6; D000685:Serum Amyloid A Protein; D014409:Tumor Necrosis Factor-alpha; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3