title: Development and characterization of a monoclonal antibody against Ochratoxin B and its application in ELISA.
abstract: A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC(50)) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
authors: Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany. alexandra.heussner@uni-konstanz.de
authors: Alexandra H
authors: AH
authors: Heussner
authors: Simon
authors: S
authors: Ausländer
authors: Daniel R
authors: DR
authors: Dietrich
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D001628:Beverages / Q000032:analysis; D001667:Binding, Competitive; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D009793:Ochratoxins; C025589:ochratoxin A; D012710:Serum Albumin, Bovine; C045354:ochratoxin B