title: Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies.
abstract: OBJECTIVE
  Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand-forelimb transplants performed at the University of Pittsburgh (PA, USA).
  
  
  METHODS
  The cell separation and release testing were performed at the University of Pittsburgh Cancer Institute's Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).
authors: Hillman Cancer Center, University of Pittsburgh Medical Center, 5117 Centre Avenue, Suite 2.42 Research Pavilion, Pittsburgh, PA 15213, USA. donnenbergad@upmc.edu
authors: Albert D
authors: AD
authors: Donnenberg
authors: Vijay S
authors: VS
authors: Gorantla
authors: Stefan
authors: S
authors: Schneeberger
authors: Linda R
authors: LR
authors: Moore
authors: Gerald
authors: G
authors: Brandacher
authors: Heather M
authors: HM
authors: Stanczak
authors: Eileen K
authors: EK
authors: Koch
authors: Wp Andrew
authors: WA
authors: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002102:Cadaver; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D015925:Cryopreservation; D003710:Demography; D006801:Humans; D008875:Middle Aged; D013131:Spine / Q000166:cytology*; D013997:Time Factors; D014019:Tissue Donors; D055815:Young Adult