title: Site directed biotinylation of filamentous phage structural proteins.
abstract: Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five structural proteins of the filamentous bacteriophage fd (Proteins 3, 7, 8 and 9). The in vivo and in vitro biotinylation of the various phages were compared. The production of multifunctional phages and their application as affinity reagents are demonstrated.
authors: Department of Cell Research and Immunology, George S, Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978 Israel.
authors: Larisa
authors: L
authors: Smelyanski
authors: Jonathan M
authors: JM
authors: Gershoni
mesh_terms: D000345:Affinity Labels / Q000737:chemistry; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D017104:Bacteriophage M13 / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019921:Biotinylation; D004926:Escherichia coli / Q000821:virology; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013194:Staining and Labeling; D014161:Transduction, Genetic; D015678:Viral Structural Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D000914:Antibodies, Viral; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins