title: Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias).
abstract: The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain.
authors: Global Biotherapeutics Technologies, Pfizer Inc., Foresterhill, Aberdeen AB25 2ZS, United Kingdom.
authors: Lauren E
authors: LE
authors: Smith
authors: Kathryn
authors: K
authors: Crouch
authors: Wei
authors: W
authors: Cao
authors: Mischa R
authors: MR
authors: Müller
authors: Leeying
authors: L
authors: Wu
authors: John
authors: J
authors: Steven
authors: Michael
authors: M
authors: Lee
authors: Musen
authors: M
authors: Liang
authors: Martin F
authors: MF
authors: Flajnik
authors: Heather H
authors: HH
authors: Shih
authors: Caroline J
authors: CJ
authors: Barelle
authors: Janet
authors: J
authors: Paulsen
authors: Davinder S
authors: DS
authors: Gill
authors: Helen
authors: H
authors: Dooley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007136:Immunoglobulins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012754:Sharks / Q000235:genetics / Q000276:immunology; D048251:Squalus acanthias / Q000235:genetics / Q000276:immunology*
chemical_list: D007136:Immunoglobulins