title: Serum levels of TNF-α, IL-1β, COMP, and CTX-II in patients with Kashin-Beck disease in Sichuan, China.
abstract: The aim of the study was to detect differences in serum levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), cartilage oligomeric matrix protein (COMP), type II collagen (CTX-II) between patients with Kashin-Beck disease (KBD) or osteoarthritis (OA) and to assess the correlation between these differences with the clinical grade of KBD. A total of one hundred fifty adult serum samples were collected; these samples belonged to the KBD group (n = 64), the OA group in KBD-prevalent areas (n = 47) and a healthy control group in non-KBD area (n = 39). Serum levels of TNF-α, IL-1β, COMP, and CTX-II were determined by a sandwich enzyme-linked immunosorbent assay, and the results were compared among the 3 groups (KBD/OA/normal) and between the different grades of KBD as well. The serum levels of IL-1β, TNF-α, COMP, and CTX-II were significantly higher in the KBD and OA group than the healthy adult group (P < 0.001), and TNF-α and IL-1β levels in the KBD group were similar to the OA group (for TNF-α, 14.38 ± 7.42 pg/ml vs. 12.61 ± 4.00 pg/ml, respectively, [P = 0.29]; for IL-1β, 141.53 ± 71.35 pg/ml vs. 135.61 ± 68.60 pg/ml, respectively, [P = 0.63]). However, the COMP level was significantly lower and the CTX-II level was higher in the KBD group than in the OA group (for COMP, 7.03 ± 3.11 ng/ml vs. 9.20 ± 3.51 ng/ml, respectively, [P = 0.003]; for CTX-II, 2.23 ± 0.79 ng/ml vs. 1.80 ± 0.87 ng/ml, respectively, [P = 0.026]). Moreover, no significant correlations were found between clinical grade and serum levels of TNF-α, IL-1β, COMP, and CTX-II for the 3 grades of KBD patients (P = 0.645, 0.481, 0.832, and 0.270, respectively). This study showed that serum levels of COMP in KBD patients decreased and CTX-II levels increased compared with the levels in OA patients, but TNF-α and IL-1β levels in KBD and OA group were similar. In addition, increased serum levels of TNF-α, IL-1β, COMP, and CTX-II were not associated with the KBD grade.
authors: Orthopedic Department, West China Hospital, Sichuan University, No. 37, Guo Xue Alley Chengdu, 610041 Chengdu, Sichuan, People's Republic of China. 9911122@163.com
authors: Xin
authors: X
authors: Tang
authors: Zongke
authors: Z
authors: Zhou
authors: Bin
authors: B
authors: Shen
authors: Jing
authors: J
authors: Yang
authors: Pengde
authors: P
authors: Kang
authors: Jian
authors: J
authors: Li
authors: Nicolas
authors: N
authors: Crook
authors: Qi
authors: Q
authors: Li
authors: Li
authors: L
authors: Min
authors: Fuxing
authors: F
authors: Pei
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D064236:Cartilage Oligomeric Matrix Protein; D002681:China; D024043:Collagen Type II / Q000097:blood*; D016326:Extracellular Matrix Proteins / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D057767:Kashin-Beck Disease / Q000097:blood*; D008297:Male; D064235:Matrilin Proteins; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D064236:Cartilage Oligomeric Matrix Protein; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D053583:Interleukin-1beta; D064235:Matrilin Proteins; C577553:TSP5 protein, human; D014409:Tumor Necrosis Factor-alpha