title: CDPK-driven changes in the intracellular ROS level and plant secondary metabolism.
abstract: Heterologous expression of a constitutively active calcium-dependent protein kinase (CDPK) gene was previously shown to increase secondary metabolite production in cultured cells of Rubia cordifolia, but the critical question of how CDPK activates secondary metabolism remains to be answered. In this article, we report that the expression of the Arabidopsis CDPK gene, AtCPK1, in R. cordifolia cells caused moderate and stable elevation of intracellular reactive oxygen species (ROS) levels. In contrast, the non-active, mutated AtCPK1 gene did not cause such an effect. The active AtCPK1 also increased cell size, likely by restricting cell division. These results are consistent with the model in which constitutive expression of AtCPK1 mimics the effects of elicitors, acting on secondary metabolism via the activation of ROS production.
authors: Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia. bulgakov@ibss.dvo.ru
authors: Victor P
authors: VP
authors: Bulgakov
authors: Tatiana Y
authors: TY
authors: Gorpenchenko
authors: Yuri N
authors: YN
authors: Shkryl
authors: Galina N
authors: GN
authors: Veremeichik
authors: Natalia P
authors: NP
authors: Mischenko
authors: Tatiana V
authors: TV
authors: Avramenko
authors: Sergey A
authors: SA
authors: Fedoreyev
authors: Yuri N
authors: YN
authors: Zhuravlev
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000032:analysis; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D045470:Rubia / Q000201:enzymology* / Q000235:genetics; D059010:Single-Cell Analysis
chemical_list: D000880:Anthraquinones; D029681:Arabidopsis Proteins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D011494:Protein Kinases; C525986:CPK1 protein, Arabidopsis