title: A novel dendrimeric peptide induces high level neutralizing antibodies against classical swine fever virus in rabbits.
abstract: The amino acid sequence (TAVSPTTLR, 829-837aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B(4)) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B(4) elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B(4) may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, China.
authors: Guo-Xin
authors: GX
authors: Li
authors: Yan-Jun
authors: YJ
authors: Zhou
authors: Hai
authors: H
authors: Yu
authors: Ling
authors: L
authors: Li
authors: Ya-Xin
authors: YX
authors: Wang
authors: Wu
authors: W
authors: Tong
authors: Jun-Wei
authors: JW
authors: Hou
authors: Yan-Zhao
authors: YZ
authors: Xu
authors: Jian-Ping
authors: JP
authors: Zhu
authors: Ao-Tian
authors: AT
authors: Xu
authors: Guang-Zhi
authors: GZ
authors: Tong
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000276:immunology; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology; D009500:Neutralization Tests; D011817:Rabbits; D013552:Swine; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines / Q000737:chemistry / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014765:Viral Vaccines