title: Molecular cloning, heterologous expression and functional characterization of a novel translationally-controlled tumor protein (TCTP) family member from Loxosceles intermedia (brown spider) venom.
abstract: Envenoming with brown spiders (Loxosceles genus) is common throughout the world. Cutaneous symptoms following spider bite accidents include dermonecrosis, erythema, itching and pain. In some cases, accidents can cause hypersensibility or even allergic reactions. These responses could be associated with histaminergic events, such as an increase in vascular permeability and vasodilatation. A protein that may be related to the effects of spider venom was identified from a previously obtained cDNA library of the L. intermedia venom gland. The amino acid sequence of this protein is homologous to proteins from the TCTP (translationally-controlled tumor protein) family, which are extracellular histamine-releasing factors (HRF) that are associated with the allergic reactions to parasites. Herein, we described the cloning, heterologous expression, purification and functional characterization of a novel member of the TCTP family from the Loxosceles intermedia venom gland. This recombinant protein, named LiRecTCTP, causes edema, enhances vascular permeability and is likely related to the inflammatory activity of the venom. Moreover, LiRecTCTP presents an immunological relationship with mammalian TCTPs.
authors: Department of Cell Biology, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors: Youssef B
authors: YB
authors: Sade
authors: Marianna
authors: M
authors: Bóia-Ferreira
authors: Luiza H
authors: LH
authors: Gremski
authors: Rafael B
authors: RB
authors: da Silveira
authors: Waldemiro
authors: W
authors: Gremski
authors: Andrea
authors: A
authors: Senff-Ribeiro
authors: Olga M
authors: OM
authors: Chaim
authors: Silvio S
authors: SS
authors: Veiga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects; D003001:Cloning, Molecular; D003429:Cross Reactions; D004487:Edema / Q000209:etiology; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D013111:Spider Venoms / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D013112:Spiders / Q000235:genetics / Q000378:metabolism*; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D013111:Spider Venoms; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1