title: The basic property of Lys385 is important for potentiation of the human α1 glycine receptor by ethanol.
abstract: Ethanol alters the function of several members of the Cys-loop ligand-gated ion channel superfamily. Recent studies have shown that the sensitivity of the α1 glycine receptor (GlyR) to ethanol can be affected by the state of G protein activation mediated by the interaction of Gβγ with intracellular amino acids in the GlyR. Here, we evaluated the physicochemical property of Lys385 that contributes to ethanol modulation by using mutagenesis, patch-clamp, and biochemical techniques. A conserved substitution (K385R) did not affect either the apparent glycine EC₅₀ (40 ± 1 versus 41 ± 0.5 μM) or the ethanol-induced potentiation (53 ± 5 versus 46 ± 5%) of the human α1 GlyR. On the other hand, replacement of this residue with glutamic acid (K385E), an acidic amino acid, reduced the potentiation of the GlyR to 10 ± 1%. Furthermore, mutations with a hydrophobic leucine (K385L), a hydrogen bond donor glutamine (K385Q), or a neutral residue (K385A) also reduced ethanol modulation. Finally, substitution by a large and hydrophobic residue (K385F) and deletion of 385 (Lys385_) reduced ethanol modulation to 10 ± 4 and 17 ± 0.4%, respectively. Experiments using dynamic cysteine substitution with a methanethiosulfonate reagent and homology modeling indicate that the basic property and the position of Lys385, probably because of its interaction with Gβγ, is critical for ethanol potentiation of the receptor.
authors: Laboratory of Neurophysiology, Department of Physiology, University of Concepcion, Concepcion, Chile.
authors: Patricio A
authors: PA
authors: Castro
authors: Maximiliano
authors: M
authors: Figueroa
authors: Gonzalo E
authors: GE
authors: Yevenes
authors: Loreto S
authors: LS
authors: San Martin
authors: Luis G
authors: LG
authors: Aguayo
mesh_terms: D019943:Amino Acid Substitution; D002462:Cell Membrane / Q000378:metabolism; D055598:Chemical Phenomena; D003545:Cysteine / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D055724:Electrophysiological Phenomena / Q000187:drug effects / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D005020:Ethyl Methanesulfonate / Q000031:analogs & derivatives / Q000737:chemistry; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism; D044388:GTP-Binding Protein gamma Subunits / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D008239:Lysine / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D018377:Neurotransmitter Agents / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D015742:Propofol / Q000494:pharmacology; D054730:Protein Interaction Domains and Motifs / Q000502:physiology; D018009:Receptors, Glycine / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D013499:Surface Properties; D014162:Transfection
chemical_list: C118981:G-protein Beta gamma; D044387:GTP-Binding Protein beta Subunits; D044388:GTP-Binding Protein gamma Subunits; D018377:Neurotransmitter Agents; D018009:Receptors, Glycine; D011993:Recombinant Fusion Proteins; C093171:methanethiosulfonate ethylammonium; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000431:Ethanol; D005020:Ethyl Methanesulfonate; D008239:Lysine; D003545:Cysteine; D005998:Glycine; D015742:Propofol