title: Immune safety evaluation of polymerized porcine hemoglobin (pPolyHb): a potential red blood cell substitute.
abstract: Polymerized Porcine Hemoglobin (pPolyHb), a hemoglobin-based oxygen carrier (HBOC), was developed as a potential red blood substitute for clinical applications. Assessment of its effects on the immune system is an important component of the overall safety evaluation of HBOC. For this purpose, we assessed three inflammation indicators, including complement C3a, IL-6, and TNF-? in cultured cells and in a rat model when pPolyHb was incubated or administrated with the cells/animals. Our results suggested that the levels of these three indicators were not statistically changed upon pPolyHb stimulation, indicating that pPolyHb is not immunotoxic to cells and animals in this aspect.
authors: College of Life Science, Northwest University, Xi'an, P. R. China.
authors: Hongli
authors: H
authors: Zhu
authors: Kunping
authors: K
authors: Yan
authors: Xiaodong
authors: X
authors: Dang
authors: He
authors: H
authors: Huang
authors: Erfang
authors: E
authors: Chen
authors: Bang
authors: B
authors: Chen
authors: Chao
authors: C
authors: Luo
authors: Thomas Ming Swi
authors: TM
authors: Chang
authors: Penggao
authors: P
authors: Dai
authors: Chao
authors: C
authors: Chen
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000276:immunology; D001802:Blood Substitutes / Q000009:adverse effects* / Q000737:chemistry*; D015926:Complement C3a / Q000378:metabolism; D004912:Erythrocytes; D006454:Hemoglobins / Q000009:adverse effects* / Q000737:chemistry* / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D013552:Swine / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001704:Biopolymers; D001802:Blood Substitutes; D006454:Hemoglobins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D015926:Complement C3a