title: High throughput chemical screening supports the involvement of Ca2+ in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis.
abstract: Recently, we reported the role of Arabidopsis cyclic nucleotide-gated ion channel (AtCNGC) 11 and 12 in Ca2+-dependent physiological responses. AtCNGC11 and 12 have been reported to be involved in plant immunity, but whether these channels play additional physiological roles was not clear before. Using single and double knockout mutants, we have found that these channels play significant roles in Ca2+ signaling, which mediates several physiological processes, such as gravitropic bending and senescence. Here, we conducted a high throughput, non-biased chemical screen using the gain-of-function mutant of AtCNGC11 and 12, cpr22. Our data presented here indicates that Ca2+ but not K+ channel blockers suppress AtCNGC11/12-induced lethality. Our data further suggest that AtCNGC11 and 12 are involved in Ca2+-dependent, but not K+-dependent physiological responses in planta.
authors: Department of Cell and Systems Biology, University of Toronto Toronto, ON Canada.
authors: Huda
authors: H
authors: Abdel-Hamid
authors: Kimberley
authors: K
authors: Chin
authors: Wolfgang
authors: W
authors: Moeder
authors: Keiko
authors: K
authors: Yoshioka
mesh_terms: D017209:Apoptosis; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000502:physiology*; D055786:Gene Knockout Techniques; D018522:Gravitropism; D057166:High-Throughput Screening Assays; D009154:Mutation; D057865:Plant Immunity; D011188:Potassium / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C510425:CNGC11 protein, Arabidopsis; C510426:CNGC12 protein, Arabidopsis; D054815:Cyclic Nucleotide-Gated Cation Channels; D011188:Potassium; D002118:Calcium