title: Genetic modifiers of cardiovascular phenotype caused by elastin haploinsufficiency act by extrinsic noncomplementation.
abstract: Elastin haploinsufficiency causes the cardiovascular complications associated with Williams-Beuren syndrome and isolated supravalvular aortic stenosis. Significant variability exists in the vascular pathology in these individuals. Using the Eln(+/-) mouse, we sought to identify the source of this variability. Following outcrossing of C57Bl/6J Eln(+/-), two backgrounds were identified whose cardiovascular parameters deviated significantly from the parental strain. F1 progeny of the C57Bl/6J; Eln(+/-)x129X1/SvJ were more hypertensive and their arteries less compliant. In contrast, Eln(+/-) animals crossed to DBA/2J were protected from the pathologic changes associated with elastin insufficiency. Among the crosses, aortic elastin and collagen content did not correlate with quantitative vasculopathy traits. Quantitative trait locus analysis performed on F2 C57; Eln(+/-)x129 intercrosses identified highly significant peaks on chromosome 1 (LOD 9.7) for systolic blood pressure and on chromosome 9 (LOD 8.7) for aortic diameter. Additional peaks were identified that affect only Eln(+/-), including a region upstream of Eln on chromosome 5 (LOD 4.5). Bioinformatic analysis of the quantitative trait locus peaks revealed several interesting candidates, including Ren1, Ncf1, and Nos1; genes whose functions are unrelated to elastic fiber assembly, but whose effects may synergize with elastin insufficiency to predispose to hypertension and stiffer blood vessels. Real time RT-PCR studies show background-specific increased expression of Ncf1 (a subunit of the NOX2 NAPDH oxidase) that parallel the presence of increased oxidative stress in Eln(+/-) aortas. This finding raises the possibility that polymorphisms in genes affecting the generation of reactive oxygen species alter cardiovascular function in individuals with elastin haploinsufficiency through extrinsic noncomplementation.
authors: Departments of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors: Beth A
authors: BA
authors: Kozel
authors: Russell H
authors: RH
authors: Knutsen
authors: Li
authors: L
authors: Ye
authors: Christopher H
authors: CH
authors: Ciliberto
authors: Thomas J
authors: TJ
authors: Broekelmann
authors: Robert P
authors: RP
authors: Mecham
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D001794:Blood Pressure / Q000235:genetics; D003433:Crosses, Genetic; D004549:Elastin / Q000235:genetics / Q000378:metabolism*; D057895:Haploinsufficiency; D006801:Humans; D006973:Hypertension / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D018980:Williams Syndrome / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
chemical_list: D017382:Reactive Oxygen Species; D004549:Elastin; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1