title: Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer.
abstract: BACKGROUND
  Human epididymis secretory protein 4 (HE4) has been proved to be a promising novel biomarker for the detection of epithelial ovarian carcinomas. Compared with CA125, HE4 assay demonstrated an improved ability to discriminate between pelvic mass with malignant and benign disease. Though it is well known that HE4 is overexpressed in ovarian cancer, however, the role of HE4 in the carcinogenesis and progression of ovarian cancer remains unkown.
  
  
  METHODS
  In this study, we explored the role of HE4 in the carcinogenesis and progression of ovarian cancer. We screened nine ovarian cancer cell lines for HE4 expression, and using RNA interference (RNAi), we silenced HE4 gene expression in CaoV3 and SKOV3.ip1 ovarian cancer cell lines. We assessed the effect of HE4 gene silencing on the transformed phenotype by examining the cell cycle, apoptosis, proliferation and transwell migration/invasion in vitro.
  
  
  RESULTS
  HE4 gene silencing induces G0/G1 arrest and blocks the progression from the G1 to S phase in CaoV3 and SKOV3.ip1 cells. HE4 knockdown also inhibited cell proliferation, migration and invasion in SKOV3.ip1 cells in vitro.
  
  
  CONCLUSIONS
  HE4 may be involved in the regulation of the cell cycle and promote ovarian cancer migration and invasion.
authors: Gynecological Oncology Center, Peking University People's Hospital, Beijing 100044, China.
authors: Shu-Li
authors: SL
authors: Zou
authors: Xiao-Hong
authors: XH
authors: Chang
authors: Xue
authors: X
authors: Ye
authors: Hong-Yan
authors: HY
authors: Cheng
authors: Ye-Xia
authors: YX
authors: Cheng
authors: Zhi-Jian
authors: ZJ
authors: Tang
authors: Zu-Juan
authors: ZJ
authors: Zhang
authors: Li
authors: L
authors: Gao
authors: Xin-Hua
authors: XH
authors: Chen
authors: Heng
authors: H
authors: Cui
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D018450:Disease Progression; D030501:Epididymal Secretory Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005260:Female; D020868:Gene Silencing / Q000502:physiology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D034622:RNA Interference
chemical_list: D014408:Biomarkers, Tumor; D030501:Epididymal Secretory Proteins