title: Coupling stimuli-responsive magnetic nanoparticles with antibody-antigen detection in immunoassays.
abstract: Because current homogeneous immunoassays show some limitations, particularly low sensitivity, we developed a new immunoassay to overcome these limitations. The approach was based on magnetic nanoparticles with a thermoresponsive polymer layer, a negatively charged polymer, and streptavidin-biotin-based antibody-antigen detection and yielded higher sensitivity than commonly used heterogeneous immunoassays. Because no special equipment is needed, it can be applied to currently available absorbance-based systems for high-throughput assays.
authors: Hirokazu
authors: H
authors: Nagaoka
authors: Yasunobu
authors: Y
authors: Sato
authors: Xiaomao
authors: X
authors: Xie
authors: Hideyuki
authors: H
authors: Hata
authors: Masaru
authors: M
authors: Eguchi
authors: Nobuki
authors: N
authors: Sakurai
authors: Takeshi
authors: T
authors: Watanabe
authors: Hiroshi
authors: H
authors: Saitoh
authors: Akihiko
authors: A
authors: Kondo
authors: Satoru
authors: S
authors: Sugita
authors: Noriyuki
authors: N
authors: Ohnishi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013972:Thyrotropin / Q000032:analysis / Q000276:immunology
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D000941:Antigens; D058185:Magnetite Nanoparticles; D011108:Polymers; C006903:carbopol 940; D001710:Biotin; D013972:Thyrotropin; D019809:Streptavidin