title: Sputum proteomics identifies elevated PIGR levels in smokers and mild-to-moderate COPD.
abstract: Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality around the world. However, the exact mechanisms leading to COPD and its progression are still poorly understood. In this study, induced sputum was analyzed by cysteine-specific two-dimensional difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry to identify proteins involved in COPD pathogenesis. The comparison of nonsmokers, smokers, and smokers with moderate COPD revealed 15 changed proteins with the majority, including polymeric immunoglobulin receptor (PIGR), being elevated in smokers and subjects with COPD. PIGR, which is involved in specific immune defense and inflammation, was further studied in sputum, lung tissue, and plasma by Western blot, immunohistochemistry/image analysis, and/or ELISA. Sputum PIGR was characterized as glycosylated secretory component (SC). Lung PIGR was significantly elevated in the bronchial and alveolar epithelium of smokers and further increased in the alveolar area in mild to moderate COPD. Plasma PIGR was elevated in smokers and smokers with COPD compared to nonsmokers with significant correlation to obstruction. In conclusion, new proteins in smoking-related chronic inflammation and COPD could be identified, with SC/PIGR being one of the most prominent not only in the lung but also in circulating blood.
authors: Proteomics Core Facility, Biocenter Oulu, Department of Biochemistry, University of Oulu, Oulu, Finland.
authors: Steffen
authors: S
authors: Ohlmeier
authors: Witold
authors: W
authors: Mazur
authors: Anna
authors: A
authors: Linja-Aho
authors: Noora
authors: N
authors: Louhelainen
authors: Mikko
authors: M
authors: Rönty
authors: Tuula
authors: T
authors: Toljamo
authors: Ulrich
authors: U
authors: Bergmann
authors: Vuokko L
authors: VL
authors: Kinnula
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000378:metabolism; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000378:metabolism*; D019056:Receptors, Polymeric Immunoglobulin / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D012907:Smoking / Q000097:blood / Q000378:metabolism*; D013183:Sputum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020543:Proteome; D019056:Receptors, Polymeric Immunoglobulin