title: Cytomegalovirus-specific CD8+ T cells targeting different peptide/HLA combinations demonstrate varying T-cell receptor diversity.
abstract: Cytomegalovirus (CMV) infection and reactivation pose a serious threat for patients after haematopoietic stem cell transplantation. We have previously shown that CD8(+) T cells targeting different CMV epitopes correlate with protection at different threshold frequencies in those patients. To investigate if this may relate to a different quality of these cells here we analyse the T-cell receptor diversity of pp50 (245-253)/HLA-A*0101 specific CD8(+) T cells with that of CD8(+) T cells targeting various pp65 peptides. The results from this pilot study show differences in the breadth of the T-cell receptor usage of the different cell populations. We observe for the first time that the T-cell receptor Vβ CDR3 spectratypes used by CMV pp50 (245-253)/HLA-A*0101-specific CD8(+) T cells can reach higher numbers than those used by CD8(+) T cells targeting various pp65 peptides in our patient cohort. This merits further investigation into the effectiveness of the different CMV-specific T cells and their impact on immunosenescence, which is important to eventually define the most useful source of adoptive therapy and monitoring protocols for cytomegalovirus-specific immune responses.
authors: Anthony Nolan Research Institute and University College London Medical School, UK. sandragiest-research@yahoo.de
authors: Sandra
authors: S
authors: Giest
authors: Alasdair
authors: A
authors: McWhinnie
authors: Marie-Paule
authors: MP
authors: Lefranc
authors: Ann-Margaret
authors: AM
authors: Little
authors: Sarah
authors: S
authors: Grace
authors: Stephen
authors: S
authors: Mackinnon
authors: J Alejandro
authors: JA
authors: Madrigal
authors: Paul J
authors: PJ
authors: Travers
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015331:Cohort Studies; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology; D005260:Female; D015788:HLA-A1 Antigen / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000276:immunology; D010750:Phosphoproteins / Q000276:immunology; D010865:Pilot Projects; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; C506470:HLA-A*01:01 antigen; D015788:HLA-A1 Antigen; C072217:ICP36 protein, Cytomegalovirus; D010455:Peptides; D010750:Phosphoproteins; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C065418:cytomegalovirus matrix protein 65kDa