title: [Use of real time PCR for testing Staphylococcus aureus isolates from the Iaşi Infectious Diseases Hospital].
abstract: S. aureus is capable of producing a wide spectrum of diseases and can quickly develop resistance to antibiotics. These features require a careful monitoring of these organisms, by detection of resistance genes and virulence factors, such as Panton-Valentine leukocidin (PVL).
  
  
  OBJECTIVE
  To determine the presence of mecA and pvl genes in S. aureus isolates by a Real Time-PCR technique (RT-PCR) in order to shorten the detection time.
  
  
  METHODS
  We tested 119 strains isolated from pus, using phenotypic methods for methicillin resistance characterization, according to CLSI 2008-2010 guidelines. Detection of mecA and pvl genes was done with hydrolysis probes.
  
  
  RESULTS
  The prevalence rate of methicilin resistant S. aureus (MRSA) was 40,33%, and pvl was detected in 52,08% of those strains. The results of the conventional methods for methicillin resistance detection were validated by those obtained by RT-PCR CONCLUSIONS: RT-PCR is useful in epidemiological surveillance of MRSA and PVL-producing strains and validation of test results for phenotypic resistance to oxacillin.
authors: Universităţii de Medicină şi Farmacie "Gr. T. Popa" Iaşi.
authors: Teodora
authors: T
authors: Vremeră
authors: Luminiţa Smaranda
authors: LS
authors: Iancu
authors: Cătălina
authors: C
authors: Logigan
authors: E
authors: E
authors: Năstase
authors: Egidia
authors: E
authors: Miftode
authors: Ecaterina
authors: E
authors: Enache
authors: Olivia
authors: O
authors: Dorneanu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D003428:Cross Infection / Q000453:epidemiology; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D055819:Hospitals, Isolation; D006785:Hospitals, University; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology; D046915:Penicillin-Binding Proteins; D010641:Phenotype; D011159:Population Surveillance; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012383:Romania / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; D037521:Virulence Factors; C487789:mecA protein, Staphylococcus aureus; D010068:Oxacillin