title: Mitochondrial membrane potential (ΔΨ) fluctuations associated with the metabolic states of mitochondria.
abstract: The study of the mitochondrial membrane potential (ΔΨ) is essential for an integrated appraisal of mitochondrial function, since it reflects differences in electrical potential and represents the main component of the proton electrochemical gradient accounting for more than 90% of the total available respiratory energy. Numerous methods have been used to estimate mitochondrial membrane potential (ΔΨ), including fluorescent methods and electrochemical probes. In this chapter, we describe several practical approaches that allow mitochondrial membrane potential (ΔΨ) evaluation, by using a tetraphenylphosphonium (TPP(+))-selective electrode. The main focus is given to the evaluation of ΔΨ in isolated mitochondria.
authors: MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology, University of Coimbra, Apartado 3046, Coimbra 3001-401, Portugal. palmeira@ci.uc.pt
authors: Carlos M
authors: CM
authors: Palmeira
authors: Anabela P
authors: AP
authors: Rolo
mesh_terms: D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects; D004229:Dithiothreitol / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000037:antagonists & inhibitors; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D016877:Oxidants; D010766:Phosphorylation; D051381:Rats; D019163:Reducing Agents / Q000494:pharmacology
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D016877:Oxidants; D019163:Reducing Agents; D004229:Dithiothreitol; C013289:tetraphenylphosphonium