title: Lack of association between apolipoprotein C3 gene polymorphisms and risk of coronary heart disease in a Han population in East China.
abstract: BACKGROUND
  Several polymorphisms in the apolipoprotein C3 (APOC3) gene have been found association with hypertriglyceridemia(HTG), but the link with coronary heart disease(CHD) risk between ethnicities was still controversial. Among them, reseachers paid more attentions to the promoter polymorphisms T-455C and C-482T because both of them located in insulin-responsive element (IRE) and insulin was thought to exert its action by down-regulating APOC3 gene expression. The aim of this study was to investigate the association of the two polymorphisms of APOC3 with CHD in a Han population in East China.
  
  
  METHODS
  TaqMan SNP Genotyping Assays were carried out to detect the genotypes of APOC3 gene, including the T-455C and C-482T, in 286 subjects with CHD and 325 controls without CHD. The levels of serum lipid profiles were also detected by biochemical methods.
  
  
  RESULTS
  There was no difference of genotype frequencies and allele frequencies between the CHD population and the controls(P > 0.05). Compared with the most common genotype -455TT or -482CC, the variants had neither significantly increased CHD risk, nor the lipid variables showed any statistically relevant differences in the research population. The adjusted OR of CHD were 5.67 [0.27-18.74] and 0.75 [0.20-2.73] in carriers of the APOC3 -455C and -482T variants, respectively(P > 0.05). There was also no significant difference in APOC3 haplotype distribution in CHD and controls, but there was a strong linkage disequilibrium between T-455C and C-482T with D' = 0.9293, 0.8881, respectively(P < 0.0001).
  
  
  CONCLUSIONS
  Our data did not support a relationship between the two polymorphisms of APOC3 gene and risk of CHD in the Han population in East China.
authors: Department of Laboratory Medicine, Changzheng Hospital, Second Military Medical University and Clinical Immunology Center of PLA, 415 Feng Yang Road, Shanghai 200003, People's Republic of China.
authors: Juan
authors: J
authors: Yu
authors: Jingjing
authors: J
authors: Huang
authors: Yan
authors: Y
authors: Liang
authors: Baodong
authors: B
authors: Qin
authors: Su
authors: S
authors: He
authors: Jing
authors: J
authors: Xiao
authors: Huimin
authors: H
authors: Wang
authors: Renqian
authors: R
authors: Zhong
mesh_terms: D000328:Adult; D000368:Aged; D053305:Apolipoprotein C-III / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D053305:Apolipoprotein C-III; D008055:Lipids