title: A safe and effective plant gene switch system for tissue-specific induction of gene expression in Arabidopsis thaliana and Brassica juncea.
abstract: The ability to regulate spatial and temporal expression of genes is a useful tool in biotechnology as well as studies of functional genomics. Such regulation can provide information concerning the function of a gene in a developmental context while avoiding potential harmful effects due to constitutive overexpression of the gene. A GUS gene construct that uses the ecdysone receptor-based chemically inducible system and several different tissue-specific promoters was introduced into the model plant Arabidopsis thaliana and into the crop plant Brassica juncea. Here we describe the results of studies showing that this system provides both temporal and spatial control of transgene expression, and confirm that this system is useful for tissue-specific and temporal induction of gene expression in A. thaliana and B. juncea.
authors: Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA.
authors: Jaemo
authors: J
authors: Yang
authors: M Isabel
authors: MI
authors: Ordiz
authors: Ekaterina G
authors: EG
authors: Semenyuk
authors: Brain
authors: B
authors: Kelly
authors: Roger N
authors: RN
authors: Beachy
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006834:Hydrazines / Q000494:pharmacology; D007605:Juvenile Hormones / Q000494:pharmacology; D009149:Mustard Plant / Q000235:genetics / Q000254:growth & development; D009928:Organ Specificity; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D011401:Promoter Regions, Genetic; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism
chemical_list: D006834:Hydrazines; D007605:Juvenile Hormones; D011987:Receptors, Steroid; C055469:ecdysone receptor; C406712:methoxyfenozide