title: Protein changes in the retina following experimental retinal detachment in rabbits.
abstract: OBJECTIVE
  Retinal detachment leads to the widespread cellular remodeling of the retina. The purpose of this study was to identify protein changes that accompany these cellular alterations by comparing the proteomic profiles of sham and experimentally detached rabbit retina. Elucidation of the proteins most dramatically affected by retinal detachment would add further understanding to the pathophysiology of this condition, and potentially identify therapeutic targets useful in preventing the deleterious effects of detachment, including photoreceptor cell death and the activation of non-neuronal microglial and Müller cells.
  
  
  METHODS
  Retinal detachments were induced in the right eyes of six New Zealand Red pigmented rabbits. Sham surgery was performed in the right eyes of six other rabbits that were used as controls. At seven days, the eyes were enucleated and the retinal tissue was harvested. The individual retinal samples were subjected to high resolution two-dimensional polyacrylamide gel electrophoresis. Differentially expressed protein spots were processed for identification by liquid chromatography-tandem mass spectrometry. Further investigation was undertaken with western blotting, and immunocytochemical studies on a further set of four sham and four detached retinas.
  
  
  RESULTS
  Eighteen protein spots were found to be at least twofold differentially expressed between the sham and detached retinas. These protein spots were identified as: vimentin; tubulin β-2C; fragments of α-enolase; fructose-bisphosphate aldolase A; ATP synthase subunit β; mitochondrial creatine kinase; N-terminal fragments of albumin; prohibitin; and transducin-β(1).
  
  
  CONCLUSIONS
  The differentially expressed proteins determined in this study may play an important role in the cellular responses of the retina after its detachment, subsequent ability to recover following surgical reattachment, as well as in serious complications such as subretinal fibrosis and proliferative vitreoretinopathy.
authors: Eye Pathology Section, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark. nm@doctors.org.uk
authors: Nakul
authors: N
authors: Mandal
authors: Geoffrey P
authors: GP
authors: Lewis
authors: Steven K
authors: SK
authors: Fisher
authors: Steffen
authors: S
authors: Heegaard
authors: Jan U
authors: JU
authors: Prause
authors: Morten
authors: M
authors: la Cour
authors: Henrik
authors: H
authors: Vorum
authors: Bent
authors: B
authors: Honoré
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D040901:Proteomics; D011817:Rabbits; D012160:Retina / Q000378:metabolism* / Q000503:physiopathology; D012163:Retinal Detachment / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D053719:Tandem Mass Spectrometry
chemical_list: D005136:Eye Proteins