title: Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae.
abstract: In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia colialdH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield reached 24.4 g/L and 0.18 mol/mol glycerol, respectively, and at the same time 1,3-PDO achieved 49.3 g/L with a yield of 0.43 mol/mol in 24 h. The overall yield of 3-HP plus 1,3-PDO was 0.61 mol/mol. Over-expression of the E. coli AldH also reduced the yields of by-products except for lactate. This study demonstrated the possibility of simultaneous production of 3-HP and 1,3-PDO by K. pneumoniae under anaerobic conditions without supply of vitamin B12.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors: Yanna
authors: Y
authors: Huang
authors: Zhimin
authors: Z
authors: Li
authors: Kazuyuki
authors: K
authors: Shimizu
authors: Qin
authors: Q
authors: Ye
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D053898:Biosynthetic Pathways; D002244:Carbon / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D010084:Oxidation-Reduction; D011409:Propylene Glycols / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D011409:Propylene Glycols; D019344:Lactic Acid; C041787:1,3-propanediol; D002244:Carbon; C031601:hydracrylic acid; D000444:Aldehyde Dehydrogenase; D005990:Glycerol