title: Low prevalence of Helicobacter pylori-negative gastric cancer among Japanese.
abstract: OBJECTIVE
  The true prevalence of Helicobacter pylori-negative gastric cancer (HpNGC) is unknown. We attempt to clarify the prevalence and clinicopathologic features of HpNGC in Japanese.
  
  
  METHODS
  Helicobacter pylori infection was detected by antibody titer and microscopic observation. In addition, we confirmed the lack of endoscopic atrophy and histologic gastritis. In these cases, we added urea breath test or rapid urease test to confirm the absence of H. pylori. The mucus phenotype of gastric cancer tissue was also evaluated by immunohistochemistry.
  
  
  RESULTS
  We screened 3161 gastric cancer cases from 1996 to 2010, and 21 cases were regarded as H. pylori negative. Clinically, patients with HpNGC were younger than patients with H. pylori-positive gastric cancer (controls), and revealed a lack of male dominancy. Histologically, diffuse type was frequently found. All patients examined were pepsinogen negative. Among HpNGC cases with endoscopic resection, the depressed macroscopic appearance was dominant. The prevalence of HpNGC was calculated as 0.66% (95% confidence interval = 0.41-1.01). The mucus phenotype of HpNGC was similar to that of the controls.
  
  
  CONCLUSIONS
  The prevalence of HpNGC is very low and its pathological characteristics are different from common gastric cancer.
authors: Department of Medicine and Molecular Science, Hiroshima University Hiroshima, Japan.
authors: Taiji
authors: T
authors: Matsuo
authors: Masanori
authors: M
authors: Ito
authors: Shunsuke
authors: S
authors: Takata
authors: Shinji
authors: S
authors: Tanaka
authors: Masaharu
authors: M
authors: Yoshihara
authors: Kazuaki
authors: K
authors: Chayama
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D044466:Asian People; D001944:Breath Tests; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000150:complications* / Q000453:epidemiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D015995:Prevalence; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000382:microbiology* / Q000473:pathology; D014510:Urease / Q000032:analysis
chemical_list: D000907:Antibodies, Bacterial; D014510:Urease