title: Investigation of a sulfate transfer during autohydrolysis of a fucoidan from the brown alga Fucus evanescens by tandem ESIMS.
abstract: A fucoidan from the brown alga Fucus evanescens was effectively depolymerized by autohydrolysis. Negative-ion electrospray ionization mass spectrometry (ESIMS) revealed that the mixture contained sulfated mono- and oligosaccharides with polymerization degree (DP) up to 6, having from 1 to 4 sulfate groups per molecule. The prevalence of oligosaccharides with even DP was observed. It could be explained by the tendency of the 3-linked α-L-fucopyranose residues to hydrolyze faster than 4-linked ones. The intermolecular sulfate transfer during autohydrolysis was detected by ESIMS, when equimolar quantities of D-Rib and D-Glc were added as acceptors. The products were singly-sulfated and hexose was about four times more effective as an acceptor, than pentose. It was impossible to record MS/MS spectra of the sulfate transfer products, since intensities of their ions were too low.
authors: Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, 100 Let Vladivostoku Ave. 159, 690022 Vladivostok, Russian Federation. sanastyuk@piboc.dvo.ru
authors: Stanislav D
authors: SD
authors: Anastyuk
authors: Natalia M
authors: NM
authors: Shevchenko
authors: Pavel S
authors: PS
authors: Dmitrenok
authors: Tatyana N
authors: TN
authors: Zvyagintseva
mesh_terms: D044645:Fucus / Q000737:chemistry*; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D013431:Sulfates; C007789:fucoidan