title: Gene expression of helicase antigen in patients with acute and chronic myeloid leukemia.
abstract: The aim of this study was to investigate the expression status of the helicase antigen (HAGE) transcript and its clinical significance in patients with acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). The expression of HAGE cDNA in bone marrow mononuclear cells from AML and CML patients was detected by using real-time quantitative PCR. The results indicated that overexpression of HAGE transcript (117.12% - 9842.70%, median 434.96%) was detected in 14.8% (11/74) AML patients. AML patients with HAGE cDNA expression were significantly older than those HAGE-negative patients (median 67 and 45 years, respectively, p = 0.001). HAGE cDNA expression was more frequently present among the patients with acute monoblastic leukemia (M(4) and M(5), 7 of 20, 35.0%), compared to the patients with acute non-monoblastic leukemia (M(1), M(2), M(3) and M(6), 4 of 54, 7.4%) (p = 0.007). 28.6% (8/28) cases with normal karyotypes showed HAGE cDNA overexpression, significantly higher than 7.5% (3 of 40) in those with chromosomal abnormalities (p = 0.041). Overexpression of HAGE transcript was found in 9 (34.6%) CML cases and more frequently observed at accelerated phase and blast crisis (4/4, 100%) than that at chronic phase (5/22, 22.7%) (p = 0.008). It is concluded that HAGE cDNA expression is relevant to specific subtypes of AML and to the progression of CML.
authors: Department of Hematology, Jiangsu University People Hospital, Zhenjiang, Jiangsu Province, China.
authors: Qin
authors: Q
authors: Chen
authors: Jiang
authors: J
authors: Lin
authors: Jun
authors: J
authors: Qian
authors: Dong-Ming
authors: DM
authors: Yao
authors: Wei
authors: W
authors: Qian
authors: Yun
authors: Y
authors: Li
authors: Hai-Yan
authors: HY
authors: Chai
authors: Jing
authors: J
authors: Yang
authors: Cui-Zhu
authors: CZ
authors: Wang
authors: Ming
authors: M
authors: Zhang
authors: Gao-Fei
authors: GF
authors: Xiao
mesh_terms: D001752:Blast Crisis; D001854:Bone Marrow Cells / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D059785:Karyotype; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C412324:DDX43 protein, human; D053487:DEAD-box RNA Helicases