title: Fabrication of protein micropatterns using a functional substrate with convertible protein-adsorption surface properties.
abstract: A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl-albumin) film for use in the fabrication of protein micropatterns. The adsorption of proteins with different characteristics onto cl-albumin film, including serum proteins, serum albumin, and lysozyme, was investigated using a quartz crystal microbalance. The results showed that surfaces coated with cl-albumin film are highly resistant to protein adsorption, regardless of protein charge and rigidity. In addition, this adsorption-resistance property can be easily converted to promote protein adsorption by exposing the cl-albumin film to a charged polymer solution. By combining the convertible surface property of cl-albumin film and inkjet printing techniques, a precise protein micropattern was successfully fabricated on the substrate. Protein adsorption onto the wall surface of microchannels could also be suppressed or promoted by coating the surface with cl-albumin film. This approach will aid in the development of biomaterials carrying protein micropatterns, such as biosensors, biochips, and cellular scaffolds.
authors: Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. hironori-yamazoe@aist.go.jp.
authors: Hironori
authors: H
authors: Yamazoe
mesh_terms: D000327:Adsorption; D000818:Animals; D062028:Bioprinting; D002417:Cattle; D003432:Cross-Linking Reagents / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism; D044085:Microfluidics; D055616:Microtechnology / Q000379:methods*; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D009113:Muramidase