title: Screening of optimal cellulases from symbiotic protists of termites through expression in the secretory pathway of Saccharomyces cerevisiae.
abstract: For direct and efficient ethanol production from cellulosic materials, we screened optimal cellulases from symbiotic protists of termites through heterologous expression with Saccharomyces cerevisiae. 11 cellulases, belonging to glycoside hydrolase families 5, 7, and 45 endoglucanases (EGs), were confirmed to produce with S. cerevisiae for the first time. A recombinant yeast expressing SM2042B24 EG I was more efficient at degrading carboxylmethyl cellulose than was Trichoderma reesei EG I, a major EG with high cellulolytic activity.
authors: Biotechnology Laboratory, Toyota Central R&D Labs, Inc, Aichi, Japan.
authors: Nemuri
authors: N
authors: Todaka
authors: Risa
authors: R
authors: Nakamura
authors: Sigeharu
authors: S
authors: Moriya
authors: Moriya
authors: M
authors: Ohkuma
authors: Toshiaki
authors: T
authors: Kudo
authors: Haruo
authors: H
authors: Takahashi
authors: Nobuhiro
authors: N
authors: Ishida
mesh_terms: D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002480:Cellulase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D044602:Cellulases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry*; D015870:Gene Expression; D020049:Isoptera / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D055571:Secretory Pathway; D013559:Symbiosis; D014242:Trichoderma / Q000201:enzymology
chemical_list: D011994:Recombinant Proteins; D000431:Ethanol; D044602:Cellulases; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium