title: PFOS elicits transcriptional responses of the ER, AHR and PPAR pathways in Oryzias melastigma in a stage-specific manner.
abstract: Perfluorooctane sulfonate (PFOS) is widely distributed in seawater. However, the effects of PFOS on important receptors involved in the toxicity of POPs have not been fully defined, especially for the embryonic stages of marine fish. In this study, we examined the transcriptional responses and PFOS accumulation in the marine medaka embryos at the early and late developmental stages of 4 and 10 dpf upon PFOS exposure (1, 4, and 16mg/L). PFOS accumulated in the embryos, and the embryonic burdens of PFOS at 10 dpf were markedly higher than those at 4 dpf. Moreover, thirteen genes involved in three important POPs-related receptor pathways, including ER, AHR and PPAR, were cloned and investigated. The mRNA expression levels of ERα and ERγ were not significantly altered, but the estrogenic marker genes were downregulated upon PFOS exposure at 4 dpf. Conversely, ERs and related marker genes all were significantly upregulated at 10 dpf. The expressions of ARNT and cyp1a were both upregulated at 4 dpf, while no obvious changes were detected at 10 dpf. The expressions of cyp19a and cyp19b were regulated by PFOS in a stage-specific manner. PFOS produced different effects on three isoforms of PPAR. PPARα and PPARβ were first inhibited at 4 dpf and were induced at 10 dpf. PFOS did not elicit a change in PPARγ expression at either stage. In conclusion, this study showed that PFOS has an estrogenic activity and endocrine-disruptive properties. Meanwhile, PFOS could elicit transcriptional responses on POPs-related pathways in a stage-specific manner.
authors: Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China.
authors: Chao
authors: C
authors: Fang
authors: Xinlong
authors: X
authors: Wu
authors: Qiansheng
authors: Q
authors: Huang
authors: Yanyan
authors: Y
authors: Liao
authors: Liangpo
authors: L
authors: Liu
authors: Ling
authors: L
authors: Qiu
authors: Heqing
authors: H
authors: Shen
authors: Sijun
authors: S
authors: Dong
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity; D029941:Fish Proteins / Q000235:genetics; D005466:Fluorocarbons / Q000633:toxicity*; D008018:Life Cycle Stages / Q000187:drug effects; D009990:Oryzias / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D017738:Alkanesulfonic Acids; D052244:Endocrine Disruptors; D029941:Fish Proteins; D005466:Fluorocarbons; D047492:Peroxisome Proliferator-Activated Receptors; D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; C076994:perfluorooctane sulfonic acid