title: Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana.
abstract: Physalis peruviana, commonly known as Cape gooseberry, is an Andean Solanaceae fruit with high nutritional value and interesting medicinal properties. In the present study we report the development and characterization of microsatellite loci from a P. peruviana commercial Colombian genotype. We identified 932 imperfect and 201 perfect Simple Sequence Repeats (SSR) loci in untranslated regions (UTRs) and 304 imperfect and 83 perfect SSR loci in coding regions from the assembled Physalis peruviana leaf transcriptome. The UTR SSR loci were used for the development of 162 primers for amplification. The efficiency of these primers was tested via PCR in a panel of seven P. peruviana accessions including Colombia, Kenya and Ecuador ecotypes and one closely related species Physalis floridana. We obtained an amplification rate of 83% and a polymorphic rate of 22%. Here we report the first P. peruviana specific microsatellite set, a valuable tool for a wide variety of applications, including functional diversity, conservation and improvement of the species.
authors: Plant Molecular Genetics Laboratory, Center of Biotechnology and Bioindustry, Colombian Corporation for Agricultural Research (CORPOICA), Bogota, Colombia.
authors: Jaime
authors: J
authors: Simbaqueba
authors: Pilar
authors: P
authors: Sánchez
authors: Erika
authors: E
authors: Sanchez
authors: Victor Manuel
authors: VM
authors: Núñez Zarantes
authors: Maria Isabel
authors: MI
authors: Chacon
authors: Luz Stella
authors: LS
authors: Barrero
authors: Leonardo
authors: L
authors: Mariño-Ramírez
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D031965:Ribes / Q000235:genetics*; D059467:Transcriptome; D020506:Untranslated Regions
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D020506:Untranslated Regions