title: [The gene ontology and electro localization of ovine skin derived EST-SSR markers].
abstract: Abstract: In order to study the potential gene function of ovine EST-SSR markers, nine original EST of Ovine Skin Derived polymorphic EST-SSR loci, which were developed in an early study by our lab, were ontology annotated and Electro localized. The results revealed that the original ESTs of the six loci had high homology with known genes and three of them probably played an important role in wool traits. Compared with its cDNA library, 8 loci were located on chromosomes of cattle. The homology of chromosomes between cattle and sheep was estimated based on the similarity coefficients calculated by positioning markers. Additionally, NJ clustering tree was establishedto serve for electro localization of ovine EST-SSR markers. Finally, 8 EST-SSR markers were successfully positioned on ovine chromosomes. The results from this study not only provide references for further studies on genetic mapping, in silico cloning of key genes for wool traits, but also are helpful to the researchs of chromosome evolution in animal.
authors: College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. wangzunbao@163.com
authors: Zun-Bao
authors: ZB
authors: Wang
authors: Zong-Sheng
authors: ZS
authors: Zhao
authors: Peng
authors: P
authors: Yu
authors: Hong-Bin
authors: HB
authors: Wu
authors: Qian
authors: Q
authors: Ban
authors: Yao-Wei
authors: YW
authors: Liang
authors: Wei
authors: W
authors: Zheng
mesh_terms: D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012756:Sheep / Q000145:classification / Q000235:genetics*; D012867:Skin / Q000166:cytology
chemical_list: D005819:Genetic Markers