title: Human platelet-rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells.
abstract: In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96-well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1-100% PRP significantly (p < 0.05) stimulate the migration of CSP compared to untreated controls. Histological staining of proteoglycan and immuno-staining of type II collagen indicated that progenitors stimulated with PRP show significantly increased cartilage matrix formation compared to untreated progenitors. Real-time gene expression analysis of typical chondrocyte marker genes as well as osteogenic and adipogenic markers like osteocalcin and fatty acid binding protein showed that PRP induces the chondrogenic differentiation sequence of human progenitors in high-density pellet cultures, while osteogenic or adipogenic differentiation was not evident. These results suggest that human PRP may enhance the migration and stimulate the chondrogenic differentiation of human subchondral progenitor cells known from microfracture.
authors: TransTissue Technologies GmbH, Charitéplatz 1, 10117 Berlin, Germany.
authors: Jan Philipp
authors: JP
authors: Krüger
authors: Sylvia
authors: S
authors: Hondke
authors: Michaela
authors: M
authors: Endres
authors: Axel
authors: A
authors: Pruss
authors: Alberto
authors: A
authors: Siclari
authors: Christian
authors: C
authors: Kaps
mesh_terms: D000328:Adult; D000368:Aged; D002454:Cell Differentiation / Q000187:drug effects; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects* / Q000502:physiology; D024043:Collagen Type II / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D024043:Collagen Type II; D003470:Culture Media; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; D011509:Proteoglycans; D015675:Osteocalcin