title: Characterization of Phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants.
abstract: This study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. Results showed that bacterial treatment followed by wetland plants (Phragmites cummunis) resulted 94.5% and 96.0% reduction in BOD and COD values, respectively. The PCR-RFLP analysis showed the presence of Stenotrophomonas, Enterobacter, Pantoea, Acinetobacter and Klebsiella sp., as dominant rhizosphere bacterial communities which play an important role in degradation and decolorization of PMDE in wetland treatment system. Further, the LC-MS-MS and other spectrophotometric analysis have shown that most of the pollutants detected in untreated PMDE were diminished from bacteria and wetland plant treated PMDE indicating that bacteria and wetland plant rhizosphere microbes utilized them as carbon, nitrogen and energy source. While, methylbenzene, furfuryl alcohol, and 4-vinyl-2-methoxyphenol were detected as metabolites in bacteria and hexadecanol in wetland plant rhizosphere treated PMDE.
authors: Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae Bareli Road, Lucknow-226 025 (U.P.), India. rc_microitrc@yahoo.co.in
authors: Ram
authors: R
authors: Chandra
authors: Ram Naresh
authors: RN
authors: Bharagava
authors: Atya
authors: A
authors: Kapley
authors: Hemant J
authors: HJ
authors: Purohit
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D002853:Chromatography, Liquid; D057168:Distillation; D007220:Industrial Waste / Q000032:analysis*; D008697:Methane; D008969:Molecular Sequence Data; D010802:Phylogeny; D006109:Poaceae / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D058441:Rhizosphere; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D014865:Waste Disposal, Fluid; D018508:Water Purification / Q000379:methods*; D053833:Wetlands
chemical_list: D007220:Industrial Waste; D008697:Methane