title: Pectinatus brassicae sp. nov., a Gram-negative, anaerobic bacterium isolated from salty wastewater.
abstract: A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TY(T), was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 °C and pH 6.5. Cells were resistant to up to 50 g NaCl l(-1). Strain TY(T) produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-BI-phosphohydrolase activities were positive. The major fatty acids were C(14 : 0) DMA (18.7 %), C(15 : 0) (15.4 %), anteiso-C(18 : 1) (15.2 %), C(11 : 0) (13.3 %) and summed feature 5 (C(17 : 1)ω7c and/or C(17 : 2)) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TY(T) represented a novel species of the genus Pectinatus (sequence similarity to other members of the genus ranged from 93.2 to 94.8 %). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TY(T) is proposed to represent a novel species, named Pectinatus brassicae sp. nov. (type strain TY(T) = JCM 17499(T) = DSM 24661(T)).
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Wen-Wu
authors: WW
authors: Zhang
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Ming-Xu
authors: MX
authors: Fang
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Hai-Qin
authors: HQ
authors: Tan
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Xin-Qi
authors: XQ
authors: Zhang
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Min
authors: M
authors: Wu
authors: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors: Xu-Fen
authors: XF
authors: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D045852:Pectinatus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013696:Temperature; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D062065:Wastewater