title: A new organotypic model containing dermal-type macrophages.
abstract: Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC-SIGN/CD209 and HLA-DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti-inflammatory cytokine IL-10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage-fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages.
authors: Nicolas
authors: N
authors: Bechetoille
authors: Hortense
authors: H
authors: Vachon
authors: Amandine
authors: A
authors: Gaydon
authors: Aurelie
authors: A
authors: Boher
authors: Thomas
authors: T
authors: Fontaine
authors: Evelyne
authors: E
authors: Schaeffer
authors: Marion
authors: M
authors: Decossas
authors: Valérie
authors: V
authors: André-Frei
authors: Christopher G
authors: CG
authors: Mueller
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008863:Microspheres; D009000:Monocytes / Q000378:metabolism; D010587:Phagocytosis / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D054457:Tissue Scaffolds; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000098902:CD163 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D016753:Interleukin-10; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D000098902:CD163 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C508609:IL10 protein, human