title: Distinct hydration properties of wild-type and familial point mutant A53T of α-synuclein associated with Parkinson's disease.
abstract: The propensity of α-synuclein to form amyloid plays an important role in Parkinson's disease. Three familial mutations, A30P, E46K, and A53T, correlate with Parkinson's disease. Therefore, unraveling the structural effects of these mutations has basic implications in understanding the molecular basis of the disease. Here, we address this issue through comparing details of the hydration of wild-type α-synuclein and its A53T mutant by a combination of wide-line NMR, differential scanning calorimetry, and molecular dynamics simulations. All three approaches suggest a hydrate shell compatible with a largely disordered state of both proteins. Its fine details, however, are different, with the mutant displaying a somewhat higher level of hydration, suggesting a bias to more open structures, favorable for protein-protein interactions leading to amyloid formation. These differences disappear in the amyloid state, suggesting basically the same surface topology, irrespective of the initial monomeric state.
authors: Institute of Enzymology, Hungarian Academy of Sciences, Budapest, Hungary.
authors: E
authors: E
authors: Hazy
authors: M
authors: M
authors: Bokor
authors: L
authors: L
authors: Kalmar
authors: A
authors: A
authors: Gelencser
authors: P
authors: P
authors: Kamasa
authors: K-H
authors: KH
authors: Han
authors: K
authors: K
authors: Tompa
authors: P
authors: P
authors: Tompa
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D050505:Mutant Proteins / Q000737:chemistry*; D010300:Parkinson Disease / Q000378:metabolism*; D017354:Point Mutation / Q000235:genetics*; D017434:Protein Structure, Tertiary; D013696:Temperature; D014867:Water / Q000737:chemistry*; D051844:alpha-Synuclein / Q000737:chemistry* / Q000235:genetics*
chemical_list: D050505:Mutant Proteins; D051844:alpha-Synuclein; D014867:Water