title: Influence of mesodermal Fgf8 on the differentiation of neural crest-derived postganglionic neurons.
abstract: The interaction between the cranial neural crest (NC) and the epibranchial placode is critical for the formation of parasympathetic and visceral sensory ganglia, respectively. However, the molecular mechanism that controls this intercellular interaction is unknown. Here we show that the spatiotemporal expression of Fibroblast growth factor 8 (Fgf8) is strategically poised to control this cellular relationship. A global reduction of Fgf8 in hypomorph embryos leads to an early loss of placode-derived sensory ganglia and reduced number of NC-derived postganglionic (PG) neurons. The latter finding is associated with the early loss of NC cells by apoptosis. This loss occurs concurrent with the interaction between the NC and placode-derived ganglia. Conditional knockout of Fgf8 in the anterior mesoderm shows that this tissue source of Fgf8 has a specific influence on the formation of PG neurons. Unlike the global reduction of Fgf8, mesodermal loss of Fgf8 leads to a deficiency in PG neurons that is independent of NC apoptosis or defects in placode-derived ganglia. We further examined the differentiation of PG precursors by using a quantitative approach to measure the intensity of Phox2b, a PG neuronal determinant. We found reduced numbers and immature state of PG precursors emerging from the placode-derived ganglia en route to their terminal target areas. Our findings support the view that global expression of Fgf8 is required for early NC survival and differentiation of placode-derived sensory neurons, and reveal a novel role for mesodermal Fgf8 on the early differentiation of the NC along the parasympathetic PG lineage.
authors: Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, Texas 78249, USA.
authors: Yiju
authors: Y
authors: Chen
authors: Anne M
authors: AM
authors: Moon
authors: Gary O
authors: GO
authors: Gaufo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D051524:Fibroblast Growth Factor 8 / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D009432:Neural Crest / Q000196:embryology*; D009475:Neurons, Afferent / Q000502:physiology*; D017777:Parasympathetic Fibers, Postganglionic / Q000196:embryology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C496457:Fgf8 protein, mouse; D018398:Homeodomain Proteins; C109220:NBPhox protein; D014157:Transcription Factors; D051524:Fibroblast Growth Factor 8