title: An improved method for rat intubation and thymectomy.
abstract: BACKGROUND
  Thymokidney has been reported as an approach for a vascularized thymus for transplantation to induce donor specific tolerance. A completely thymectomized model which ensures that the obtained thymus is not injured has not been developed yet and it would be useful for evaluating autologous thymokidney function in rats.
  
  
  METHODS
  Adult Sprague-Dawley male rats weighing 150 - 300 g (n = 30) underwent non-invasive intubation with the assistance of an improved self-made wedge-shaped cannula made from a 2-ml plastic syringe and transillumination from the anterior tracheal area by an operation spotlight. The rats then received a thoracotomy while their breathing was supported by a small animal ventilator, and both lobes of the thymus were entirely extirpated under a 10× microscope. The postoperative survival rate of the rats was recorded, and changes in the T-cell reservoir from 9 of 30 rats within 21 days after surgery were monitored using flow cytometry. The complete thymectomy rate was confirmed by autopsy and histological examination on 21 days post-operation.
  
  
  RESULTS
  The postoperative survival rate of rats was 100%. The exsected thymus was free of injury and the rate of complete thymectomy was 100%.
  
  
  CONCLUSIONS
  This model has a stable survival rate and complete thymectomy is able to be achieved. The obtained thymus tissue is free of injury and can be used for transplantation.
authors: Department of Renal Transplantation, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors: Ning
authors: N
authors: Na
authors: Da-qiang
authors: DQ
authors: Zhao
authors: Zheng-yu
authors: ZY
authors: Huang
authors: Liang-qing
authors: LQ
authors: Hong
mesh_terms: D000818:Animals; D007442:Intubation, Intratracheal / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013908:Thoracotomy / Q000379:methods; D013934:Thymectomy / Q000379:methods*; D013950:Thymus Gland / Q000601:surgery