title: Relationships between tumour necrosis factor-α, interleukin-12B and interleukin-10 gene polymorphisms and hepatitis B in Chinese Han haemodialysis patients.
abstract: OBJECTIVE
  To investigate the possible association of gene polymorphisms of tumour necrosis factor (TNF)-α (-238 and -308), interleukin (IL)-10 (-592 and -819) and 3' untranslated region (3'UTR) of the IL12B (-1188) and hepatitis B in Chinese Han haemodialysis (HD) patients.
  
  
  METHODS
  The genotyping of TNF-α -238 and -308, IL-10 -592 and -819 and 3'UTR of the IL12B were performed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method.
  
  
  RESULTS
  The TNF-α-238 A allele, the IL12B 3'UTR C/C, C/A genotypes were associated with decreased susceptibility to hepatitis B viral infection (P = 0.047, P = 0.003 and P = 0.001 respectively). The frequencies of IL-10-592 A/A genotype, IL-10-819 T/T genotype were lower in the HBV persistence group (P = 0.029 and P = 0.019) than those in the virus clearance group.
  
  
  CONCLUSIONS
  TNF-α and IL12B 3'UTR gene polymorphisms may be associated with HBV susceptibility and IL-10 gene polymorphisms may be related to the HBV persistence infection in Chinese Han HD patients.
authors: Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors: Cuiyu
authors: C
authors: Wang
authors: Xiaoli
authors: X
authors: Zhang
authors: Bei
authors: B
authors: Zhu
authors: Dalan
authors: D
authors: Hu
authors: Jianqing
authors: J
authors: Wu
authors: Rongbin
authors: R
authors: Yu
authors: Weihong
authors: W
authors: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006509:Hepatitis B / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics* / Q000276:immunology; D006515:Hepatitis B virus / Q000472:pathogenicity*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D006435:Renal Dialysis; D018570:Risk Assessment; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C508609:IL10 protein, human; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10