title: Preliminary studies for cephamycin C purification technique.
abstract: A study was made for purification of cephamycin C from fermentation of Streptomyces clavuligerus. Initially, the culture broth was clarified by microfiltration and ultrafiltration, after which the resulting permeates were subjected to nonspecific adsorption and ion-exchange chromatography on resin columns. The antibiotic activity was measured by the biological method at each stage by assaying its activity against the Escherichia coli ESS, super sensitive to β-lactam antibiotic. The purification processes were assessed in relation to the variables affecting each step. The purification efficiency by nonspecific adsorption was monitored by UV spectrophotometry, while the ion-exchange adsorption fractions were assessed by NMR spectroscopy. Some of the fractions obtained during purification were also analyzed by mass spectrometry (LC/MS and LC/MS/MS) to identify the cephamycin C molecule. These preliminary results proved the process feasibility.
authors: Verdartis Desenvolvimento Biotecnológico Ltda-ME, Rua dos Técnicos s/n sala 5, Incubadora Supera Campus USP, Riberão Preto, SP, Brazil.
authors: Alvaro de Baptista
authors: Ade B
authors: Neto
authors: Maritza Catalina Condori
authors: MC
authors: Bustamante
authors: Jaine Honorata Hortolan Luiz de
authors: JH
authors: Oliveira
authors: Ana Cláudia
authors: AC
authors: Granato
authors: Carolina
authors: C
authors: Bellão
authors: Alberto Coli
authors: AC
authors: Badino
authors: Alberto Colli Junior
authors: AC
authors: Badino
authors: Marlei
authors: M
authors: Barboza
authors: Carlos Osamu
authors: CO
authors: Hokka
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002513:Cephamycins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000187:drug effects; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011994:Recombinant Proteins; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000737:chemistry*; D047090:beta-Lactams / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D002513:Cephamycins; D011994:Recombinant Proteins; D047090:beta-Lactams; C024925:cephamycin C