title: The yolk cell of the zebrafish blastula harbors functional apoptosis machinery.
abstract: We recently described the implication of the Bcl-2 related antiapoptotic Nrz protein during early zebrafish development. Nrz knock-down induces calcium-dependent cytoskeleton remodeling leading to margin constriction and premature embryo lethality. In the YSL, nrz knock-down embryos exhibit some typical features of apoptosis such as mitochondrial transmembrane potential loss and cytochrome c release. However, downstream caspase-3 activation has not been detected so far. Here, we report that the YSL contains fully functional apoptotic machinery that can activate caspase-3 following zBax ectopic expression. Furthermore, we present evidence that caspase-3 activation is actually detectable in nrz knock-down embryos when premature margin constriction is prevented.
authors: CRCL U1052 INSERM; UMS 3453 CNRS-Université Lyon 1; Centre Léon Bérard; Lyon, France.
authors: Nikolay
authors: N
authors: Popgeorgiev
authors: CRCL U1052 INSERM; UMS 3453 CNRS-Université Lyon 1; Centre Léon Bérard; Lyon, France.
authors: Julien
authors: J
authors: Prudent
authors: CRCL U1052 INSERM; UMS 3453 CNRS-Université Lyon 1; Centre Léon Bérard; Lyon, France.
authors: Benjamin
authors: B
authors: Bonneau
authors: CRCL U1052 INSERM; UMS 3453 CNRS-Université Lyon 1; Centre Léon Bérard; Lyon, France.
authors: Germain
authors: G
authors: Gillet