title: Effect of neem leaf extract and neem oil on Penicillium growth, sporulation, morphology and ochratoxin A production.
abstract: In vitro trials were conducted to evaluate the effect of Azadirachtaindica (neem) extracts on mycelial growth, sporulation, morphology and ochratoxin A production by P. verrucosum and P. brevicompactum. The effect of neem oil extract from seeds and leaf was evaluated at 0.125; 0.25 and 0.5% and 6.25 and 12.5 mg/mL, respectively, in Yeast Extract Sucrose (YES) medium. Ochratoxin A production was evaluated by a thin-layer chromatography technique. Oil extracts exhibited significant (p ≤ 0.05) reduction of growth and sporulation of the fungi. No inhibition of ochratoxin A production was observed. Given its accessibility and low cost, neem oil could be implemented as part of a sustainable integrated pest management strategy for plant disease, as it has been shown to be fungitoxic by inhibition of growth and sporulation.
authors: Departamento de Bioquímica, Universidade Estadual de Maringá. Avenida Colombo, 5790, 87020-900, Maringá, PR, Brazil. simonegmossini@yahoo.com.br
authors: Simone A G
authors: SA
authors: Mossini
authors: Carla C
authors: CC
authors: Arrotéia
authors: Carlos
authors: C
authors: Kemmelmeier
mesh_terms: D031601:Azadirachta; D002855:Chromatography, Thin Layer; D010407:Penicillium; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D010936:Plant Extracts