title: HIV type 1 genetic diversity in newly diagnosed Cuban patients.
abstract: Knowledge of the genetic diversity of HIV-1 constitutes a fundamental premise in the epidemiological surveillance. In the present study, the HIV-1 genetic variability from 142 Cuban patients who were diagnosed with HIV-1 infection during 2009 and 2010 was determined. HIV-1 subtypes were determined by partial RT-PCR and sequencing of the HIV-1 pol gene. The phylogenetic analysis showed that 47 (33.1 %) samples were subtypes B and 95 (66.9 %) were non-B subtypes, where G, H, and C subtypes, as well as the recombinant forms CRF19_cpx, CRF18_cpx, and CRFs BG, were included. The circulation of CRF05_DF was detected for the first time in Cuba. The analyses of recombinants showed the presence of recombinant CRF18_cpx/CRF19_cpx. The study confirms the high genetic diversity of HIV-1 and the circulation of new genetic variants in the studied population, which indicates the importance of maintaining constant epidemiological surveillance in Cuba.
authors: AIDS Research Laboratory, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque, Cuba. liuberyans@infomed.sld.cu
authors: Liuber Y
authors: LY
authors: Machado
authors: Madeline
authors: M
authors: Blanco
authors: Marta
authors: M
authors: Dubed
authors: Héctor M
authors: HM
authors: Díaz
authors: Nancy M
authors: NM
authors: Ruiz
authors: Neysi
authors: N
authors: Váldes
authors: Dania
authors: D
authors: Romay
authors: Leonor I
authors: LI
authors: Lobaina
mesh_terms: D000328:Adult; D003462:Cuba; D005260:Female; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny