title: Development of microsatellite loci for the endangered species Pityopsis ruthii (Asteraceae).
abstract: OBJECTIVE
  Microsatellite loci were developed for the endangered species Pityopsis ruthii and will permit genetic and conservation studies of the species.
  
  
  RESULTS
  A microsatellite-enriched library was used to develop 12 polymorphic microsatellite loci for P. ruthii. The loci amplified perfect and imperfect repeats with three to seven alleles per locus. Observed heterozygosity ranged from 0.05 to 0.80 and expected heterozygosity ranged from 0.23 to 0.75.
  
  
  CONCLUSIONS
  These microsatellite loci provide a sufficient set of markers for further investigation of population genetics of P. ruthii.
authors: Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, 37996-4560, USA. pwadl@utk.edu
authors: Phillip A
authors: PA
authors: Wadl
authors: Adam J
authors: AJ
authors: Dattilo
authors: Brian E
authors: BE
authors: Scheffler
authors: Robert N
authors: RN
authors: Trigiano
mesh_terms: D019659:Asteraceae / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D056727:Endangered Species; D056426:Genetic Loci / Q000235:genetics; D005821:Genetic Techniques; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers