title: Absence of kl-vs variant of klotho gene in Iranian cardiac patients (comparison to the world populations).
abstract: OBJECTIVE
  Klotho has an important role in development of coronary artery (CAD) disease. A functional variant of klotho gene (kl-vs) has been found as an independent risk factor for early-onset occult coronary artery disease (CAD) in previous studies. The Frequency of this variant was not known in Iranian population. We have examined the allele frequency of the kl-vs variant in a case-control study in an Iranian population.
  
  
  RESULTS
  Genotyping for kl-vs variant was carried out in N=107 individuals including N=54 cases and N=53 control who all underwent coronary angiogram for CAD evaluation. Patients with >50% stenosis in vessels considered as case groups (or CAD<formula>^{+}</formula>) and patients with normal vessels (or CAD<formula>^{-}</formula>) as controls. The frequency of kl-vs variant was determined in these patients using PCR-RFLP technique. None of the individual was carrying the kl-vs mutation in our samples. The frequency of kl-vs mutation was significantly different from previous studies in different populations.
  
  
  CONCLUSIONS
  The kl-vs variant seems to be scare found in the Iranian population in comparison to other populations reported previously. Klotho gene might be a candidate gene of atherosclerosis in some populations but not in Iranian population. Further studies are required to examine the frequency of kl-vs variant in other populations from the Middle East.
authors: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Iran. tavakkolybazzazj@tums.ac.ir
authors: Javad
authors: J
authors: Tavakkoly-Bazzaz
authors: Ozra
authors: O
authors: Tabatabaei-Malazy
authors: Mohammad
authors: M
authors: Tajmir-Riahi
authors: Daryoosh
authors: D
authors: Javidi
authors: Manizhe
authors: M
authors: Izadi
authors: Maryam
authors: M
authors: Shahrabi-Farahani
authors: Parvin
authors: P
authors: Amiri
authors: Mahsa M
authors: MM
authors: Amoli
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D007492:Iran; D000090265:Klotho Proteins; D008297:Male; D008875:Middle Aged; D017422:Sequence Analysis, DNA
chemical_list: D005966:Glucuronidase; D000090265:Klotho Proteins