title: Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes.
abstract: Hydrogen-rich electrolyzed warm water (HW) was prepared at 41°C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and+184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold more abundantly by HW application for 3-5 days than RW in OUMS-36 fibroblasts, and localized preferentially around the nuclei as shown by immunostain. HW application significantly prevented cell death and DNA damages such as nuclear condensation and fragmentation in UVA-irradiated HaCaT keratinocytes as estimated by WST-1 and Hoechst 33342 assays. HW significantly suppressed UVA-induced generation of intracellular superoxide anion radicals in both the cell lines according to NBT assay. Wrinkle repression was clinically assessed using a HW-bathing. Six Japanese subjects were enrolled in a trial of HW-bathing (DH, 0.2-0.4 ppm) every day for 3 months. HW-bathing significantly improved wrinkle in four subjects on the back of neck on 90th day as compared to 0 day. Thus, HW may serve as daily skin care to repress UVA-induced skin damages by ROS-scavenging and promotion of type-I collagen synthesis in dermis.
authors: Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan.
authors: Shinya
authors: S
authors: Kato
authors: Yasukazu
authors: Y
authors: Saitoh
authors: Keizou
authors: K
authors: Iwai
authors: Nobuhiko
authors: N
authors: Miwa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism*; D004249:DNA Damage; D020405:Dermis / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D006859:Hydrogen / Q000737:chemistry; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D013481:Superoxides / Q000378:metabolism; D013696:Temperature; D014466:Ultraviolet Rays; D014867:Water / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D017382:Reactive Oxygen Species; D014867:Water; D013481:Superoxides; D006859:Hydrogen