title: BLT2 phosphorylation at Thr355 by Akt is necessary for BLT2-mediated chemotaxis.
abstract: BLT2, a low-affinity leukotriene B(4) (LTB(4)) receptor, is a member of the G protein-coupled receptor family and is involved in multiple cellular responses, including chemotaxis. Despite its biological significance, the mechanisms of BLT2 regulation, especially by protein kinases, are poorly characterised. In this study, we found that Akt phosphorylates BLT2 at its C-terminal Thr(355) residue and that this event is critical for BLT2-mediated chemotactic responses. In addition, we found that Rac1 stimulation and subsequent reactive oxygen species (ROS) production lie downstream of BLT2 phosphorylation, thus mediating chemotaxis.
authors: College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors: Jun-Dong
authors: JD
authors: Wei
authors: Joo-Young
authors: JY
authors: Kim
authors: Jae-Hong
authors: JH
authors: Kim
mesh_terms: D000818:Animals; D016466:CHO Cells; D002633:Chemotaxis; D006224:Cricetinae; D006801:Humans; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D018102:Receptors, Leukotriene B4 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013912:Threonine / Q000235:genetics* / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: C485878:LTB4R2 protein, human; D017382:Reactive Oxygen Species; D018102:Receptors, Leukotriene B4; D013912:Threonine; D051057:Proto-Oncogene Proteins c-akt; D020830:rac1 GTP-Binding Protein