title: Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series.
abstract: This paper describes the calibration method of the tilt and azimuth angles of specimen using a digital protractor and a laser autocollimator for alignment of electron tomography. It also suggests an easy method to check whether the specimen is tilted by 180.0°, and whether the azimuth angle is 0.0°; the method involves the use of two images of a rod-shaped specimen collected before and after a 180.0° tilt. The method is based on the assumption that these images are symmetric about the tilt axis when the azimuth angle is 0.0°. In addition, we used an experiment to demonstrate the effect of the incorrect angles on reconstructed images and simulated the image quality against distance away from tilt axis.
authors: National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8565, Japan. misa-hayashida@aist.go.jp
authors: Misa
authors: M
authors: Hayashida
authors: Shinya
authors: S
authors: Terauchi
authors: Toshiyuki
authors: T
authors: Fujimoto