title: Molecular identification of the sperm selection involved porcine sperm binding glycoprotein (SBG) as deleted in malignant brain tumors 1 (DMBT1).
abstract: Porcine sperm binding glycoprotein (SBG) is involved in sperm-oviduct interaction. Here we use mass spectrometry to identify SBG, finding peptides corresponding to deleted in malignant brain tumors 1 (DMBT1), at scavenger receptor cysteine-rich (SRCR) and CUB domains. RT-PCR allowed the cloning of unique sequences, belonging to porcine DMBT1. Western blot and immunofluorescence of oviductal tissues using anti-SBG and anti-hDMBT1 antibodies showed identical results. The biochemical characteristics of both proteins are coincident. We conclude that porcine SBG is an oviductal form of DMBT1, and thus assign this protein a novel location and function.
authors: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Área Biología, Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR) Suipacha 590-Rosario (S2002LRK), Santa fe, Argentina.
authors: Juan Manuel
authors: JM
authors: Teijeiro
authors: María Lorena
authors: ML
authors: Roldán
authors: Patricia Estela
authors: PE
authors: Marini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D018076:DNA, Complementary; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029589:Seminal Plasma Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; D011956:Receptors, Cell Surface; D029589:Seminal Plasma Proteins; C425042:spermadhesin