title: A reporter protein for meat integrity.
abstract: A method for distinguishing fresh from thawed frozen samples of porcine and bovine meat is proposed.Crude soluble extracts from fresh (stored at +4 °C for 0-3 days) or deep frozen (at -80 °C) meat samples from pork and beef, were assayed by blocking ELISA to estimate m-MDH (mitochondrial malate dehydrogenase) titres. As a rule, a separate aliquot of each test sample was placed at -20 °C until frozen and subsequently thawed for the preparation of the extract whose analysis gave the 'self reference' m-MDH titre. For a given meat sample calculation of the tau index, 'τ', was proposed as the ratio between the self reference value and the original m-MDH titre. The 95% tolerance for τ for fresh and thawed meat, suggests that the method is suitable for this kind of analysis.
authors: Department of Chemistry, Chemical Engineering and Materials, I-67040 Monteluco di Roio (AQ, Italy.
authors: V
authors: V
authors: Salfi
authors: O
authors: O
authors: Portanti