title: Quercetin production from rutin by a thermostable β-rutinosidase from Pyrococcus furiosus.
abstract: Pyrococcus furiosus β-glucosidase converted rutin to quercetin and rutinose disaccharide with a ratio of 1:1, with no glucose, L-rhamnose, and isoquercitrin, indicating that the enzyme is a β-rutinosidase. The specific activity for flavonoid glycosides followed the order of isoquercitrin > quercitrin > rutin. The conversion of rutin to quercetin was optimal at pH 5.0 and 95°C in the presence of 0.5% dimethyl sulfoxide with a half-life of 101 h, a k(cat) of 1.6 min(-1), and a K(m) of 0.3 mM. Under the improved conditions, the enzyme produced 6.5 mM quercetin from 10 mM rutin after 150 min, with a molar yield of 65% and a productivity of 2.6 mM/h. This productivity is the highest reported thus far among enzymatic transformations.
authors: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
authors: Hyun-Koo
authors: HK
authors: Nam
authors: Seung-Hye
authors: SH
authors: Hong
authors: Kyung-Chul
authors: KC
authors: Shin
authors: Deok-Kun
authors: DK
authors: Oh
mesh_terms: D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D020027:Pyrococcus furiosus / Q000201:enzymology*; D011794:Quercetin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012431:Rutin / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; C416847:beta-rutinosidase