title: Structures of building blocks in clusters of sweetpotato amylopectin.
abstract: φ,β-Limit dextrins of domains and clusters of sweetpotato amylopectin were subjected to extensive hydrolysis by Bacillus amyloliquefaciens α-amylase to release building blocks and reveal the internal structures of clusters. The composition of building blocks was analyzed by size-fractionation, gel permeation chromatography, and high performance anion exchange chromatography. Different domains and clusters had structurally similar building blocks with around three chains per building block and internal chain length around 2.9. Singly branched and doubly branched building blocks were the largest and second largest groups in the clusters. Type A clusters had more large building blocks and contained 5-6 blocks per cluster with an inter-block chain length (IB-CL) of 7.0, whereas type B clusters had less large building blocks and contained 3-4 blocks per cluster with IB-CL 7.9. Models on how the building blocks could be organized into type A and type B clusters are discussed.
authors: School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China. fzhu5@yahoo.com
authors: Fan
authors: F
authors: Zhu
authors: Harold
authors: H
authors: Corke
authors: Per
authors: P
authors: Åman
authors: Eric
authors: E
authors: Bertoft
mesh_terms: D000687:Amylopectin / Q000737:chemistry* / Q000378:metabolism; D001407:Bacillus / Q000145:classification / Q000201:enzymology; D002236:Carbohydrate Conformation; D006868:Hydrolysis; D027723:Ipomoea batatas / Q000737:chemistry*; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000687:Amylopectin; D000516:alpha-Amylases