title: Methylation status of lamin A/C in gastric cancer cell lines.
abstract: OBJECTIVE
  Epigenetic regulations play a role in the development and progression of cancer. Therefore, discovering novel epigenetically regulated genes could provide useful information in understanding cancer. Lamin A/C is an intermediate filament protein whose expression is reported to be suppressed in tissues of gastro-intestinal malignancies. We examined expression of lamin A/C in gastric and colorectal cancer cell lines and its association with DNA methylation.
  
  
  METHODS
  The methylation status of CpG island in 19 gastric, 5 colorectal cancer cells and 1 normal colon cell line were examined with methylation-specific PCR using paired methylated and unmethylated primers. The level of mRNA expression of lamin A/C was detected using RT-PCR.
  
  
  RESULTS
  Eighteen gastric cancer cell lines showed 95% unmethylation of lamin A/C and 1 cell line showed partial methylation. In colorectal cancer, only 1 out of 5 cancer cell lines (20%) was partially methylated and the remaining cell lines, including 1 normal colon cell line was unmethylated. With RT-PCR, all cell lines demonstrated mRNA expression of lamin A/C regardless of methylation status.
  
  
  CONCLUSIONS
  We observed that the expression of lamin A/C was not suppressed in gastrointestinal cancer cell lines different from hematologic malignant cells and it is not regulated through DNA methylation.
authors: Cancer Metastasis Research Center, Seoul, Korea.
authors: Won Suk
authors: WS
authors: Lee
authors: Jae Joon
authors: JJ
authors: Jung
authors: Hei-Cheul
authors: HC
authors: Jeung
authors: Se Won
authors: SW
authors: Noh
authors: Bong Kyeong
authors: BK
authors: Oh
authors: Ki Yeol
authors: KY
authors: Kim
authors: Tae Soo
authors: TS
authors: Kim
authors: Hyun Cheol
authors: HC
authors: Chung
authors: Jae Kyung
authors: JK
authors: Roh
authors: Sun Young
authors: SY
authors: Rha
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics; D018899:CpG Islands; D019175:DNA Methylation; D015536:Down-Regulation; D006801:Humans; D034904:Lamin Type A / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C494847:LMNA protein, human; D034904:Lamin Type A