title: Regioselective glucosidation of trans-resveratrol in Escherichia coli expressing glucosyltransferase from Phytolacca americana.
abstract: A glucosyltransferase (GT) of Phytolacca americana (PaGT3) was expressed in Escherichia coli and purified for the synthesis of two O-β-glucoside products of trans-resveratrol. The reaction was moderately regioselective with a ratio of 4'-O-β-glucoside: 3-O-β-glucoside at 10:3. We used not only the purified enzyme but also the E. coli cells containing the PaGT3 gene for the synthesis of glycoconjugates. E. coli cell cultures also have other advantages, such as a shorter incubation time compared with cultured plant cells, no need for the addition of exogenous glucosyl donor compounds such as UDP-glucose, and almost complete conversion of the aglycone to the glucoside products. Furthermore, a homology model of PaGT3 and mutagenesis studies suggested that His-20 would be a catalytically important residue.
authors: Department of Biological Sciences, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi 753-8511, Japan.
authors: Shin-ichi
authors: S
authors: Ozaki
authors: Hiroya
authors: H
authors: Imai
authors: Tomoya
authors: T
authors: Iwakiri
authors: Takehiro
authors: T
authors: Sato
authors: Kei
authors: K
authors: Shimoda
authors: Toru
authors: T
authors: Nakayama
authors: Hiroki
authors: H
authors: Hamada
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005964:Glucosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D029604:Phytolacca americana / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000077185:Resveratrol; D013237:Stereoisomerism; D013267:Stilbenes / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D013267:Stilbenes; D005964:Glucosyltransferases; D000077185:Resveratrol