title: The effect of varying carboxylate ligation on the electronic environment of N2O(x) (x = 1-3) nonheme iron: a DFT analysis.
abstract: Mononuclear nonheme iron oxygenase (MNO) enzymes contain a subclass of metalloproteins capable of catalyzing the O(2)-dependent hydroxylation of unactivated substrates at a ferrous ion center coordinated to a highly conserved His-His-Glu/Asp motif. These enzymes, which utilize additional reducing equivalents obtained from the decarboxylation of a coordinated α-ketoglutarate (αKG) cofactor, do not readily interact with O(2) in the absence of αKG binding. Density functional theory calculations with the B3LYP functional were performed to gain insight into the electrochemical behavior of three sets of Fe(II/III) complexes containing a core N, N, O facial binding motif in which the number of carboxylate ligands was systematically altered, to provide one, two (cis) or three (fac) labile sites. The calculated trend in Fe(II/III) reduction potentials was observed to parallel that observed in cyclic voltammetry experiments, showing a decrease in potential (stabilized oxidized state) with increasing carboxylate ligation. This trend does not appear to be the result of differential charge on the metal complex. Changes in the redox-active molecular orbital (RAMO) energy due to covalent effects dominate across the series of complexes when chloride is modeled as the labile ligand, with the π anti-bonding nature of the RAMO being an important factor. With water molecules as the labile ligands, however, a much steeper redox dependence on the number of carboxylate ligands is observed and this effect seems to be largely electrostatic in origin. Differential relaxation of the occupied molecular orbitals in the ferric complexes appears to contribute to the redox trend as well. Finally, these observations are placed in the context of MNO enzyme mechanisms.
authors: Department of Chemistry, Boston University, Boston, MA 02215, USA.
authors: Patrick J
authors: PJ
authors: Cappillino
authors: Joshua S
authors: JS
authors: McNally
authors: Feng
authors: F
authors: Wang
authors: John P
authors: JP
authors: Caradonna
mesh_terms: D002264:Carboxylic Acids / Q000737:chemistry*; D018360:Crystallography, X-Ray; D049308:Dioxygenases / Q000737:chemistry* / Q000378:metabolism; D004583:Electrons; D007656:Ketoglutaric Acids / Q000737:chemistry; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D009589:Nitrogen Oxides / Q000737:chemistry*; D019159:Nonheme Iron Proteins / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D007656:Ketoglutaric Acids; D008024:Ligands; D009589:Nitrogen Oxides; D019159:Nonheme Iron Proteins; D049308:Dioxygenases; D010100:Oxygen