title: Investigation of the effect of hyperglycemia on intracerebral hemorrhage by proteomic approaches.
abstract: Intracerebral hemorrhage (ICH) is associated with high mortality and disability, and hyperglycemia worsens the clinical and neurological outcomes of patients with ICH. In this study, we utilized proteomic approaches to investigate the role of hyperglycemia in ICH. Hyperglycemia was induced by intraperitoneal injection of streptozotocin (STZ) in adult Sprague-Dawley male rats; ICH was induced by stereotaxic infusion of collagenase/heparin into the right striatum. It was observed that the size of induced hemorrhage was significantly larger in the hyperglycemic group (n=6 in each group). On the first day after ICH, an apparent decrease in the bilateral grasp was also observed for the lesioned hyperglycemic rats compared with normoglycemic ones. When employing 2-DE and MS to examine the proteomes of perihematomal and control regions in individual hyperglycemic and normoglycemic rats, eight differentially expressed protein targets were identified. Most noteworthy, in response to ICH significant increase of albumin was ubiquitously observed in the brains of normoglycemic rats but not in the brains of hyperglycemic rats. Coincidentally, more significant neuronal apoptosis were found in the perihematomal regions of hyperglycemic rats. These observations described suggest the protection role of albumin in acute stage of ICH, which may be dependent on different blood sugar levels.
authors: Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
authors: Cheng-Di
authors: CD
authors: Chiu
authors: Tze-Yung
authors: TY
authors: Chen
authors: Li-Te
authors: LT
authors: Chin
authors: Chiung-Chyi
authors: CC
authors: Shen
authors: Jie
authors: J
authors: Huo
authors: Shin-Yi
authors: SY
authors: Ma
authors: Han-Min
authors: HM
authors: Chen
authors: Chi-Hong
authors: CH
authors: Chu
mesh_terms: D000418:Albumins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001786:Blood Glucose; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000139:chemically induced / Q000150:complications* / Q000378:metabolism; D017364:Collagenases; D015870:Gene Expression; D006493:Heparin; D006943:Hyperglycemia / Q000139:chemically induced / Q000150:complications* / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000235:genetics / Q000378:metabolism; D008297:Male; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism
chemical_list: D000418:Albumins; D001786:Blood Glucose; C550693:PEBP1 protein, rat; D049488:Phosphatidylethanolamine Binding Protein; D006493:Heparin; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase; D017364:Collagenases; C497342:ATP synthase beta subunit, rat; D025261:Mitochondrial Proton-Translocating ATPases; D010751:Phosphopyruvate Hydratase; D007791:Lactoylglutathione Lyase