title: Survivin-deltaEx3: a novel biomarker for diagnosis of papillary thyroid carcinoma.
abstract: BACKGROUND
  The most important problem in the case of thyroid nodules is the lack of suitable criteria for detecting malignant thyroid tumors from other nodules in the early stage. Variable expressions level of survivin, an inhibitory protein in apoptotic pathway, and its splice variants in malignant carcinoma versus well-differentiated normal tissues candidate them as reliable biomarkers in cancers.
  
  
  OBJECTIVE
  To semi-quantitative detection of survivin and its splice variant, survivin-deltaEx3, in thyroid nodules.
  
  
  METHODS
  We evaluated the expression level of mentioned biomarkers in thyroid nodules including carcinoma.
  
  
  METHODS
  Samples were collected from 61 thyroid nodules including malignant, adenoma, non-tumoral (goiter and thyroidities) as well as non-neoplastic normal tissues. Transcriptional levels were measured using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and the results were normalized to β2microglubin (β2m) gene.
  
  
  METHODS
  Independent sample t-test was used to assess the significant variation of expression between different groups.
  
  
  RESULTS
  Our data for a first time revealed that survivin-deltaEx3 is significantly up-regulated from normal to malignant thyroid carcinoma tissues (approximately ten fold).
  
  
  CONCLUSIONS
  High expression level of survivin and survivin-deltaEx3 in malignant papillary thyroid carcinoma suggested survivin gene expression and its splice variant, survivin-deltaEx3, can be potential new markers in diagnosis of human papillary thyroid carcinoma.
authors: Department of Biology-Genetics, University of Tabriz; Department of Genetics, Tarbiat Modares University, Tehran, Iran.
authors: Somayeh
authors: S
authors: Vandghanooni
authors: Morteza
authors: M
authors: Eskandani
authors: Vahid
authors: V
authors: Montazeri
authors: Monireh
authors: M
authors: Halimi
authors: Esmaeil
authors: E
authors: Babaei
authors: Mohamad Ali Hosseinpour
authors: MA
authors: Feizi
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma; D002291:Carcinoma, Papillary / Q000175:diagnosis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis*; D016606:Thyroid Nodule / Q000175:diagnosis
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D000077022:Survivin