title: Extractive fermentation for improved production and recovery of lipase derived from Burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems.
abstract: An extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular Burkholderia cepacia lipase. A 10% (w/w) solution of ethylene oxide-propylene oxide (EOPO) with a molecular mass of 3900 g/mol and pH 8.5, a 200 rpm speed, and 30 °C were selected as the optimal conditions for lipase production (55 U/ml). Repetitive batch fermentation was performed by continuous replacement of the top phase every 24h, which resulted in an average cell growth mass of 4.7 g/L for 10 extractive batches over 240 h. In scaling-up the process, a bench-scale bioreactor was tested under the conditions that had been optimized in flasks. The production rate and recovery yield were higher in the bioreactor compared to fermentation performed in flasks.
authors: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors: Pau Loke
authors: PL
authors: Show
authors: Chin Ping
authors: CP
authors: Tan
authors: Mohd
authors: M
authors: Shamsul Anuar
authors: Arbakariya
authors: A
authors: Ariff
authors: Yus Aniza
authors: YA
authors: Yusof
authors: Soo Kien
authors: SK
authors: Chen
authors: Tau Chuan
authors: TC
authors: Ling
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D016956:Burkholderia cepacia / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008049:Lipase / Q000096:biosynthesis* / Q000302:isolation & purification; D044367:Phase Transition / Q000187:drug effects*; D011095:Polyethylenes / Q000494:pharmacology*; D011126:Polypropylenes / Q000494:pharmacology*; D059027:Recycling; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D011095:Polyethylenes; D011126:Polypropylenes; D014867:Water; C078661:UCON 50-HB-5100; D008049:Lipase