title: Reversine increases multipotent human mesenchymal cells differentiation potential.
abstract: Among different human stem cell sources, adult mesenchymal stem cells from bone marrow (BMSCs), and more recently from adipose tissues (ASCs), have shown their capability to differentiate into a variety of different cell types, including osteoblasts, adipocytes, and muscle cells. However, mesenchymal stem cell differentiation toward certain cell types (including skeletal and cardiac muscle), while shown to be achievable, still suffers of low yields and needs to be greatly improved before any therapeutic application could be foreseen. A possible way of achieving this goal is by using a chemical-pharmacological approach to increase stem cell plasticity. Along this line, we envisioned the possibility of pre-treating BMSCs and ASCs with reversine, a synthetic purine that has been shown to induce adult cells de-differentiation. In the current study we tested reversine effects on both BMSCs and ASCs to increase their differentiation toward osteoblasts, smooth and skeletal muscle cells. Reversine pre-treatment, at very low concentration (50 nM), caused a marked increase in the differentiation yields of both BMSCs and ASCs.
authors: IRCCS Policlinico San Donato, San Donato Milanese, Italy.
authors: E
authors: E
authors: Conforti
authors: E
authors: E
authors: Arrigoni
authors: M
authors: M
authors: Piccoli
authors: S
authors: S
authors: Lopa
authors: L
authors: L
authors: de Girolamo
authors: A
authors: A
authors: Ibatici
authors: A
authors: A
authors: Di Matteo
authors: G
authors: G
authors: Tettamanti
authors: A T
authors: AT
authors: Brini
authors: L
authors: L
authors: Anastasia
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology*
chemical_list: D009025:Morpholines; D011687:Purines; C484369:2-(4-morpholinoanilino)-6-cyclohexylaminopurine