title: Mononuclear cell adenosine deaminase and CD26/dipeptidylpeptidase-IV activities are sensitive markers of reperfusion during percutaneous transluminal angioplasty.
abstract: During ischaemia, the extracellular level of adenosine increases, which has cytotoxic effects. In endothelium, cell surface adenosine deaminase (ADA) complexing CD26 is coordinately induced during ischaemia as part of an adaptative response by eliminating adenosine. We examined whether a similar mechanism exists for mononuclear cells. We studied mononuclear cell surface ADA (MCADA) and dipeptidyl-peptidase IV activity (DPPIV) of membrane CD26 during percutaneous transluminal coronary angioplasty (PTCA) as a model of ischaemia-reperfusion. Enzymatic activities were compared with levels of ischaemia-modified albumin (IMA), a marker of ischaemia-reperfusion.
  
  
  RESULTS
  Patients (15 men and 5 women) with non-ST segment elevation acute coronary syndrome related to a stenosis of proximal left anterior descending artery were prospectively included before revascularization. MCADA, DPPIV and IMA were measured before PTCA (T0) then 15 (T15) and 120 (T120) minutes after reperfusion. Fifteen healthy control subjects were enrolled. At T0, MCADA and IMA levels were higher in patients than in controls. MCADA decreased at T15 (median, IQR: 8.2 [7.6-9.8] IU) relative to T0 (11.25 [10-13.5] IU, p<0.01) and remained low at T120. DPPIV decreased at T15 (0.9 [0.7-1.1] AU) relative to T0 (1.05 [0.99-1.48] AU; p<0.01) and remained low at T120. IMA level increased only at T120. MCADA and DPPIV were correlated. Our findings are that MCADA and DPPIV decreased rapidly after angioplasty, suggesting that both catalysts are early markers of reperfusion.
  
  
  CONCLUSIONS
  MCADA and DPPIV are sensitive and early markers of ischaemia-reperfusion process during PTCA.
authors: Department of Cardiology, Timone University Hospital, Marseille, France.
authors: Pierre-Julien
authors: PJ
authors: Moro
authors: Jacques
authors: J
authors: Quilici
authors: Roch
authors: R
authors: Giorgi
authors: Thomas
authors: T
authors: Cuisset
authors: Youlet
authors: Y
authors: By
authors: Alain
authors: A
authors: Boussuges
authors: Yves
authors: Y
authors: Jammes
authors: Jean-Louis
authors: JL
authors: Bonnet
authors: Jean
authors: J
authors: Ruf
authors: Emmanuel
authors: E
authors: Fenouillet
authors: Régis
authors: R
authors: Guieu
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood*; D000368:Aged; D017130:Angioplasty / Q000379:methods; D015415:Biomarkers / Q000097:blood; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D015425:Myocardial Reperfusion / Q000379:methods; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D015415:Biomarkers; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; C548482:ADA protein, human; D000243:Adenosine Deaminase