title: Synthesis and evaluation of the europium(III) and zinc(II) complexes as luminescent bioprobes in high content cell-imaging analysis.
abstract: Novel phenanthroline derivatives and their europium(III) and zinc(II) complexes have been prepared in up to 92%. In contrast to the stable zinc complexes, the europium compounds exhibit a strong luminescence in THF solution. However, quenching of the emission is observed in DMSO indicating complete dissociation of the complexes back to free ligands in this solvent. (1)H NMR studies of the Eu(III)-complexes 5 and 6 also confirmed the existence of different states depending on the solvent used. Moreover, it was found that compound 5 is stable in EtOH-PBS solutions; here a strong signal in the emission spectra corresponding to the europium ion was detected. No spectral changes were observed for the zinc(II) complexes, they were shown to be stable in the media. These metal complexes can be used as fluorescence markers for the diagnosis of oesophageal squamous carcinoma (OE21) cells at low concentrations. Cell images were acquired using the compounds 5, 7-9 as luminescent agents. The first images were taken already after 20 min incubation time at a very low concentration range (0.7-1.6 μM).
authors: SFI Tetrapyrrole Laboratory, School of Chemistry, Trinity College Dublin, Dublin, Ireland. sergeevn@tcd.ie
authors: Natalia N
authors: NN
authors: Sergeeva
authors: Marion
authors: M
authors: Donnier-Marechal
authors: Gisela
authors: G
authors: Vaz
authors: Anthony M
authors: AM
authors: Davies
authors: Mathias O
authors: MO
authors: Senge
mesh_terms: D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry; D004121:Dimethyl Sulfoxide; D004355:Drug Stability; D000431:Ethanol; D005063:Europium; D005663:Furans; D006801:Humans; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry; D049449:Luminescence; D049408:Luminescent Agents / Q000138:chemical synthesis* / Q000737:chemistry; D012997:Solvents; D015032:Zinc
chemical_list: D056831:Coordination Complexes; D005663:Furans; D007202:Indicators and Reagents; D049408:Luminescent Agents; D012997:Solvents; D000431:Ethanol; C018674:tetrahydrofuran; D005063:Europium; D015032:Zinc; D004121:Dimethyl Sulfoxide