title: Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells.
abstract: This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a(+)CD11R1(high) and CD4(+) cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
authors: Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. haruki@bios.tohoku.ac.jp.
authors: Shoichi
authors: S
authors: Hosoya
authors: Julio
authors: J
authors: Villena
authors: Tomoyuki
authors: T
authors: Shimazu
authors: Masanori
authors: M
authors: Tohno
authors: Hitomi
authors: H
authors: Fujie
authors: Eriko
authors: E
authors: Chiba
authors: Takeshi
authors: T
authors: Shimosato
authors: Hisashi
authors: H
authors: Aso
authors: Yoshihito
authors: Y
authors: Suda
authors: Yasushi
authors: Y
authors: Kawai
authors: Tadao
authors: T
authors: Saito
authors: Susana
authors: S
authors: Alvarez
authors: Shuji
authors: S
authors: Ikegami
authors: Hiroyuki
authors: H
authors: Itoh
authors: Haruki
authors: H
authors: Kitazawa
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells; D001419:Bacteria / Q000276:immunology / Q000378:metabolism*; D018929:Cell Culture Techniques; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology*; D005786:Gene Expression Regulation; D007422:Intestines / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D011070:Poly I-C / Q000008:administration & dosage; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000235:genetics / Q000276:immunology*; D051196:Toll-Like Receptor 3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D051196:Toll-Like Receptor 3; D011070:Poly I-C