title: HSV-1 gene expression from reactivated ganglia is disordered and concurrent with suppression of latency-associated transcript and miRNAs.
abstract: In cell cultures, HSV-1 replication is initiated by recruitment by virion protein 16 of transcriptional factors and histone-modifying enzymes to immediate early (α) gene promoters. HSV establishes latent infections characterized by suppression of viral gene expression except for latency-associated transcripts (LATs) and miRNAs. The latent virus reactivates in stressed neurons. A fundamental question is how reactivation initiates in the absence of virion protein 16. We report the following findings in the ganglion explant model. (i) Anti-nerve growth factor antibody accelerated the reactivation of latent virus. Viral mRNAs were detected as early as 9 h after explantation. (ii) After explantation the amounts of viral mRNAs increased whereas amounts of miRNAs and LATs decreased. The decrease in miRNAs and LATs required ongoing protein synthesis, raising the possibility that LAT and miRNAs were degraded by a viral gene product. (iii) The expression of viral genes in explanted ganglia was disordered rather than sequentially ordered as in infected cells in culture. These findings suggest that in reactivating ganglia gene expression is totally derepressed and challenge the current models in that establishment of or exit from latency could not be dependent on the suppression or activation of single or small clusters of viral genes. Finally, miRNAs and LATs reached peak levels 9-11 d after corneal inoculation, thus approximating the pattern of virus replication in these ganglia. These findings suggest that the patterns of accumulation of LATs and miRNAs reflect many different stages in the infection of neurons.
authors: Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, Chicago, IL 60637, USA.
authors: Te
authors: T
authors: Du
authors: Guoying
authors: G
authors: Zhou
authors: Bernard
authors: B
authors: Roizman
mesh_terms: D000818:Animals; D003315:Cornea / Q000821:virology; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D005724:Ganglia / Q000378:metabolism* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000821:virology; D017735:Virus Latency / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral; C477253:latency associated transcript, herpes simplex virus-1; D003609:Dactinomycin; D020932:Nerve Growth Factor