title: OmpA-like protein influences cell shape and adhesive activity of Tannerella forsythia.
abstract: Tannerella forsythia, a gram-negative fusiform rod, is implicated in several types of oral anaerobic infections. Most gram-negative bacteria have OmpA-like proteins that are homologous to the OmpA protein in Escherichia coli. We identified an OmpA-like protein in T. forsythia encoded by the tf1331 gene as one of the major proteins by mass spectrometric analysis. Two-dimensional, diagonal electrophoresis showed that the OmpA-like protein formed a dimeric or trimeric structure via intermolecular disulfide bonds. A biotin labeling experiment revealed that a portion of the protein was exposed on the cell surface, even though T. forsythia possesses an S-layer at the outermost cell surface. Using a tf1331-deletion mutant, we showed that the OmpA-like protein affected cell morphology. The length of the mutant cell was reduced almost by half. Cell swelling was observed in more than 40% of the mutant cells. Moreover, the mutant exhibited decreased adhesion to fibronectin, retarded autoaggregation, and reduced cell surface hydrophobicity. These results suggest that the OmpA-like protein in T. forsythia plays an important role in cellular integrity and adhesive function.
authors: Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
authors: T
authors: T
authors: Abe
authors: Y
authors: Y
authors: Murakami
authors: K
authors: K
authors: Nagano
authors: Y
authors: Y
authors: Hasegawa
authors: K
authors: K
authors: Moriguchi
authors: N
authors: N
authors: Ohno
authors: K
authors: K
authors: Shimozato
authors: F
authors: F
authors: Yoshimura
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis; D001439:Bacteroides / Q000166:cytology / Q000502:physiology*; D018441:Biofilms; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005109:Extracellular Matrix / Q000382:microbiology; D005353:Fibronectins / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008562:Membrane Glycoproteins / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D005353:Fibronectins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C078167:OMPA outer membrane proteins