title: Helicobacter pylori infection recruits bone marrow-derived cells that participate in gastric preneoplasia in mice.
abstract: OBJECTIVE
  Studies in animal models have shown that bone marrow-derived cells (BMDC) could be involved in the formation of carcinomas of the upper gastrointestinal tract, including gastric carcinoma. Most gastric carcinomas in humans have been associated with chronic infection with Helicobacter pylori; we investigated the bacteria's potential to induce premalignant lesions in mice and studied the kinetics of BMDC settlement in the gastric epithelium.
  
  
  METHODS
  C57BL/6J female chimeric mice with BMDCs from male donors that express green fluorescent protein were infected with human-derived and mouse-adapted strains of H pylori and followed. We assessed development of pathologic features and recruitment of BMDC to the gastric mucosa using immunohistochemistry and fluorescent in situ hybridization analyses of gastric tissue sections.
  
  
  RESULTS
  Infection of mice with different strains of H pylori led to the development of chronic inflammation, hyperplasia, and mucinous metaplasia, and, later in life, of pseudointestinal metaplasia and dysplasia. After 1 year, gastric glands that contained green fluorescent protein-positive male cells were detected in 50%-90% of female chimeric mice infected with H pylori strains; the presence of these glands correlated with the development of pseudointestinal metaplasia. Twenty-two percent of H pylori-induced dysplastic lesions were composed of glands that contained epithelial BMDCs.
  
  
  CONCLUSIONS
  H pylori infection leads to development of chronic inflammation, hyperplasia, metaplasia, and dysplasia, as well as the recruitment and accumulation of BMDC in the gastric epithelial mucosa. Nearly 25% of dysplastic lesions include cells that originate from the BM.
authors: INSERM U853, Bordeaux, France. christine.varon@u-bordeaux2.fr
authors: Christine
authors: C
authors: Varon
authors: Pierre
authors: P
authors: Dubus
authors: Frédéric
authors: F
authors: Mazurier
authors: Corinne
authors: C
authors: Asencio
authors: Lucie
authors: L
authors: Chambonnier
authors: Jonathan
authors: J
authors: Ferrand
authors: Alban
authors: A
authors: Giese
authors: Nathalie
authors: N
authors: Senant-Dugot
authors: Martina
authors: M
authors: Carlotti
authors: Francis
authors: F
authors: Mégraud
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology*; D016481:Helicobacter Infections / Q000150:complications / Q000473:pathology*; D016480:Helicobacter pylori; D006965:Hyperplasia / Q000382:microbiology; D017404:In Situ Hybridization, Fluorescence; D007249:Inflammation / Q000382:microbiology / Q000473:pathology; D008297:Male; D008679:Metaplasia / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D014411:Neoplastic Stem Cells / Q000473:pathology*; D011230:Precancerous Conditions / Q000382:microbiology* / Q000473:pathology; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology