title: In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization.
abstract: We identified a gene product of At5g19500 (At5g19500p) from Arabidopsis thaliana that is homologous to EcTyrP, a tyrosine-specific transporter from Escherichia coli. Computational analyses of the amino acid sequence of At5g19500p predicted 11 transmembrane domains (TMDs) and a potential plastid targeting signal at its amino terminus. As a first step toward understanding the possible role of At5g19500p in plant cells, we attempted to determine the localization of At5g19500p by an in vitro chloroplastic import assay using At5g19500p translated in a cell-free wheat germ system (Madin et al., Proc. Natl. Acad. Sci. USA, 97, 559-564 (2000)), followed by subfractionation of the chloroplasts. At5g19500p was successfully imported into chloroplasts, and the newly transported mature form of At5g19500p was recovered from the inner envelope membrane.
authors: The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Japan.
authors: Kwanchanok
authors: K
authors: Sattasuk
authors: Akira
authors: A
authors: Nozawa
authors: Yuzuru
authors: Y
authors: Tozawa
authors: Yoshimi
authors: Y
authors: Kakinuma
authors: Mitsuru
authors: M
authors: Akita
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002474:Cell-Free System; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D017385:Sequence Homology
chemical_list: D026905:Amino Acid Transport Systems; D029681:Arabidopsis Proteins; C570735:At5g19500 protein, Arabidopsis