title: A new assay for endonuclease/methyltransferase activities based on graphene oxide.
abstract: A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
authors: Department of Chemistry, KAIST, Daejeon, 305-701, Republic of Korea.
authors: Jieon
authors: J
authors: Lee
authors: Young-Kwan
authors: YK
authors: Kim
authors: Dal-Hee
authors: DH
authors: Min
mesh_terms: D004247:DNA / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry*; D006868:Hydrolysis; D008780:Methyltransferases / Q000378:metabolism*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D006108:Graphite; D004247:DNA; D008780:Methyltransferases; D004720:Endonucleases