title: Identification of lactobacilli residing in chicken ceca with antagonism against Campylobacter.
abstract: Bacteriocins produced by Lactobacillus salivarius have been recently recognized as a natural means to control Campylobacter and Salmonella in live poultry. This finding is of relevance since Campylobacter jejuni and Campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. In the present work, lactic acid bacteria (LAB) isolated from the cecum of twenty Tunisian chickens were identified and those isolates with antagonism against Campylobacter were further characterized. Following their preliminary confirmation as LAB, 150 strains were identified by combining morphological criteria, biochemical tests, and molecular methods, the latter inluding intergenic 16S- 23S PCR, specific lactobacilli PCR, and a biphasic approach. Most of the LAB isolated belonged to the genus Lactobacillus, among them Lb. sakei (33.3%), Lb. salivarius (19.4%), Lb. reuteri (8.6%), and Lb. curvatus (8.6%). The other LAB strains included those of the genus  Weissella (16.7%),  Enterococcus faecalis (5.3%),  Leuconostoc mesenteroides (2.7%), Lactococcus graviae (2.7%), and Streptococcus sp. (2.7%). The Lactobacilli strains were tested for their antagonism against C. jejuni and C. coli. The activity of three of them, Lb. salivarius SMXD51,  Lb. salivarius MMS122, and  Lb. salivarius MMS151, against the aforementioned target strains could be ascribed to the production of bacteriocins.
authors: LUNAM University, Oniris, UMR1014 Secalim, Nantes, France.
authors: Soumaya
authors: S
authors: Messaoudi
authors: Gilles
authors: G
authors: Kergourlay
authors: Albert
authors: A
authors: Rossero
authors: Mounir
authors: M
authors: Ferchichi
authors: Hervé
authors: H
authors: Prévost
authors: Djamel
authors: D
authors: Drider
authors: Mohamed
authors: M
authors: Manai
authors: Xavier
authors: X
authors: Dousset
mesh_terms: D000818:Animals; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology; D017000:Campylobacter coli / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D056546:Lactobacillales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014416:Tunisia
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S