title: Identification of local clinical Candida isolates using CHROMagar Candida™ as a primary identification method for various Candida species.
abstract: The objective of our study was to study the effectiveness of CHROMagar Candida™   as the primary identification method for various clinical Candida isolates, other than the   three suggested species by the manufacturer. We studied 34 clinical isolates which were   isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first   cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35ºC, then on CHROMagar plates   at 30ºC, 35ºC and 37ºC. The sensitivity of this agar to identify Candida albicans, Candida   dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and   Candida parapsilosis ranged between 25 and 100% at 30ºC, 14% and 100% at 35ºC, 56% and   100% at 37ºC. The specificity of this agar was 100% at 30ºC, between 97% and 100% at 35ºC,   92% and 100% at 37ºC. The efficiency of this agar ranged between 88 and 100% at 30ºC, 83%   and 100% at 35ºC, 88% and 100% at 37ºC. Each species also gave rise to a variety of colony   colours ranging from pink to green to blue of different colony characteristics. Therefore, the   chromogenic agar was found to be useful in our study for identifying clinical Candida isolates.
authors: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia. farida@medic.upm.edu.my
authors: P
authors: P
authors: Madhavan
authors: F
authors: F
authors: Jamal
authors: P P
authors: PP
authors: Chong
authors: K P
authors: KP
authors: Ng
mesh_terms: D002175:Candida / Q000145:classification* / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D003470:Culture Media