title: [Effect of taspine derivatives on human liver cancer SMMC7721].
abstract: OBJECTIVE
  To analyse the inhibition effect of taspine derivatives on human Liver cancer SMMC7721 cell and its mechanism.
  
  
  METHODS
  The effects of five taspine derivatives on SMMC7721 cell growth were determined by MTT. The flow cytometry was used to determine the cell cycle. The effects of Tas-D1 on the EGF and VEGF in SMMC7721 cell were determined by ELISA. The mRNA level of EGF and VEGF in SMMC7721 cell was determined by RT-PCR.
  
  
  RESULTS
  The MTT assay demonstrated that the taspine derivative Tas-D1 significantly inhibited the growth of SMMC7721 cell in a dose-dependent manner. Cell was stopped at S phase by Tas-D1. Tas-D1 inhibited the expression of EGF and VEGF and their mRNA in a dose-dependent manner (P<0.05).
  
  
  CONCLUSIONS
  The taspine derivative Tas-D1 can inhibit the growth of human Liver cancer SMMC7721 cell and change cell cycle, which may be related to the inhibition of EGF and VEGF expression.
authors: School of Medcine, Xi'an Jiaotong University, Xi'an 710061, China. zhang2008@mail.xjtu.edu.cn
authors: Yan-min
authors: YM
authors: Zhang
authors: Nan
authors: N
authors: Wang
authors: Bing-ling
authors: BL
authors: Dai
authors: Lang-chong
authors: LC
authors: He
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D028145:Caulophyllum / Q000737:chemistry; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor; C018394:taspine