title: Two-day fasting prior to intestinal ischemia-reperfusion injury on bacterial translocation in rats.
abstract: OBJECTIVE
  The aim of this study is to verify the effect of two-day fasting prior to intestinal ischemia-reperfusion (I/R) injury on bacterial translocation (BT).
  
  
  METHODS
  Mail Sprague-Dawley rats were divided into four groups: group 1, control rats that underwent sham operation only; group 2, rats fasted for two days prior to sham operation; group 3, rats that underwent occlusion of mesenteric vessels for 30 min followed by reperfusion for 4 hr; and group 4, rats fasted for two days prior to the same intestinal I/R injury as in group 3. In all groups, E. coli labeled with (99m)Tc were inoculated into the terminal ileum. Two hr after inoculation of E. coli, the rats were killed. A segment of ileum was obtained for histological examination and samples of mesenteric lymph nodes (MLNs), liver, lung, blood, and spleen were obtained for radioactivity determination.
  
  
  RESULTS
  There were no significant differences in the intestinal mucosa and radioactivity of all samples between groups 1 and 2. Group 3 showed significantly shorter mucosa and villi, and higher radioactivity of samples, except for MLNs, compared to group 1. Group 4 showed similar mucosa and villi, but significantly higher radioactivity of samples, except for MLNs, compared to group 3.
  
  
  CONCLUSIONS
  Two-day fasting without I/R injury does not cause mucosal change and BT, but in cases following intestinal I/R injury, two-day fasting increases the susceptibility of BT to systemic organs in rats.
authors: Department of Surgery College of Medicine,   Hanyang University Guri Hospital, Gyeonggi-do,   Korea.
authors: Jae Jeong
authors: JJ
authors: Park
authors: Ku-Yong
authors: KY
authors: Chung
authors: Young Soo
authors: YS
authors: Nam
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000502:physiology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004926:Escherichia coli; D005215:Fasting / Q000009:adverse effects*; D007082:Ileum / Q000382:microbiology*; D007410:Intestinal Diseases / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology*