title: Isolation & characterization of Hoechst(low) CD45(negative) mouse lung mesenchymal stem cells.
abstract: Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor formation. Taken together these studies suggest that resident lung MSC play a role during pulmonary tissue homeostasis, injury and repair during diseases such as pulmonary fibrosis (PF) and arterial hypertension (PAH).  Here we describe a technology to define a population of resident lung MSC. The definition of this population in vivo pulmonary tissue using a define set of markers facilitates the repeated isolation of a well-characterized stem cell population by flow cytometry and the study of a specific cell type and function.
authors: Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, USA.
authors: Kelsey S
authors: KS
authors: Chow
authors: DuHyun
authors: D
authors: Jun
authors: Karen M
authors: KM
authors: Helm
authors: David H
authors: DH
authors: Wagner
authors: Susan M
authors: SM
authors: Majka
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry*; D002469:Cell Separation / Q000379:methods; D003584:Cytological Techniques / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D017493:Leukocyte Common Antigens / Q000096:biosynthesis / Q000172:deficiency*; D008168:Lung / Q000166:cytology* / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D001562:Benzimidazoles; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse; C017807:bisbenzimide ethoxide trihydrochloride