title: Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination.
abstract: Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via wavefront shaping. Here, we demonstrate a pupil-segmentation based adaptive optical approach with full-pupil illumination. When implemented in a two-photon fluorescence microscope, it recovers diffraction-limited performance and improves imaging signal and resolution.
authors: Coleman Technologies, Inc., 5131 West Chester Pike, Newtown Square, Pennsylvania 19073, USA.
authors: Daniel E
authors: DE
authors: Milkie
authors: Eric
authors: E
authors: Betzig
authors: Na
authors: N
authors: Ji
mesh_terms: D000221:Adaptation, Ocular; D000465:Algorithms; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods* / Q000706:statistics & numerical data; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods / Q000706:statistics & numerical data; D055620:Optical Phenomena; D011680:Pupil / Q000502:physiology*; D012542:Scattering, Radiation