title: [Attenuation effects of compatible medicines on arsenical and lead toxicity of badu shengji san].
abstract: Badu Shengji San(BDSJS) is a traditional Chinese medicine (TCM) used for drawing out toxin, eliminating suppuration and promoting granulation. Toxic minerals such as arsenic and lead are the two most important components of BDSJS. Previous hypothesis indicated that according to the compatibility theory of TCM, the toxicity of the entire BDSJS was weaker than that of arsenic and lead, respectively. In the present study, SD rats with injured skin were treated with distilled water and different composition of BDSJS (complete formulations, compatible herbs, mineral medicine containing arsenic and lead, mineral medicine containing arsenic and mineral medicine containing lead) once a day for consecutive 2 weeks. Kidney coefficient and urinary beta-N-acetyl glucosidase (NAG) were used as the indicators of renal toxicity and the content of malondiadehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), glutathione (GSH) and metallothionein (MT) in the renal tissue were measured. Our data showed that kidney coefficient, the severity of renal pathological lesion and MT level in the kidney of the entire BDSJS group decreased significantly compared with arsenic and lead group. Additionally, the NAG content of the entire BDSJS group had the decreased trend. The kidney CuZn-SOD level of the entire BDSJS group had the increased trend, but the MDA, GSH-PX, GSH level had no obvious difference. Our results suggested that compatible herbs in BDSJS relieved renal injury induced by arsenic and lead, and the attenuation mechanism may be related to MT and CuZn-SOD, but not to MDA, GSH-PX and GSH directly.
authors: Insititute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors: Yanli
authors: Y
authors: Lu
authors: Rong
authors: R
authors: He
authors: Bo
authors: B
authors: Peng
authors: Jie
authors: J
authors: Gao
authors: Jianrong
authors: J
authors: Li
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007854:Lead / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D007854:Lead; D008315:Malondialdehyde; D008668:Metallothionein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001151:Arsenic