title: Nano/microstructural effect of hydroxyapatite nanocrystals on hepatocyte cell aggregation and adhesion.
abstract: Hepatocyte cell aggregation and adhesion to HAp nanocrystals covered with SU-8 polymer micropatterns by nano/microfabrication techniques is demonstrated. The surface roughness and wettability of the HAp nanocrystals are significantly different from those of the SU-8 polymer. QCM-D and microscopic observation clearly reveal that the cells realize the surface properties to form aggregation and preferentially adhere to the HAp nanocrystals at 2 h after seeding, indicating the importance of the microstructures as well as the interfacial phenomena at a nanometer scale.
authors: Department of Metallurgy and Ceramics Science, Graduate School of Science & Engineering, Tokyo Institute of Technology, Tokyo, Japan. tagaya.m.aa@m.titech.ac.jp
authors: Motohiro
authors: M
authors: Tagaya
authors: Tomohiko
authors: T
authors: Yamazaki
authors: Daiji
authors: D
authors: Tsuya
authors: Yoshimasa
authors: Y
authors: Sugimoto
authors: Nobutaka
authors: N
authors: Hanagata
authors: Toshiyuki
authors: T
authors: Ikoma
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D002448:Cell Adhesion; D002449:Cell Aggregation; D018360:Crystallography, X-Ray; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004852:Epoxy Compounds / Q000737:chemistry; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D055668:Photochemical Processes; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D004852:Epoxy Compounds; D017886:Durapatite