title: Artificial microvascular network: a new tool for measuring rheologic properties of stored red blood cells.
abstract: BACKGROUND
  The progressive deterioration of red blood cell (RBC) rheologic properties during refrigerated storage may reduce the clinical efficacy of transfusion of older units.
  
  
  METHODS
  This article describes the development of a microfluidic device designed to test the rheologic properties of stored RBCs by measuring their ability to perfuse an artificial microvascular network (AMVN) comprised of capillary-size microchannels arranged in a pattern inspired by the real microvasculature. In the AMVN device, the properties of RBCs are evaluated by passing a 40% hematocrit suspension of RBCs through the network and measuring the overall perfusion rate.
  
  
  RESULTS
  The sensitivity of the AMVN device to the storage-induced change in rheologic properties of RBCs was tested using five prestorage leukoreduced RBC units stored in AS-1 for 41 days. The AMVN perfusion rate for stored RBCs was 26 ± 4% (19%-30%) lower than for fresh RBCs. Washing these stored RBCs in saline improved their performance by 41 ± 6% (the AMVN perfusion rate for washed stored RBCs was still 15 ± 2% lower than for fresh RBCs).
  
  
  CONCLUSIONS
  The measurements performed using the AMVN device confirm a significant decline in the rheologic properties of RBCs in units nearing expiration and demonstrate the sensitivity of the device to these storage-induced changes. The AMVN device may be useful for testing the effect of new storage conditions, additive solutions, and rejuvenation strategies on the rheologic properties of stored RBCs in vitro.
authors: Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA.
authors: Jennie M
authors: JM
authors: Burns
authors: Xiaoxi
authors: X
authors: Yang
authors: Omid
authors: O
authors: Forouzan
authors: Jose M
authors: JM
authors: Sosa
authors: Sergey S
authors: SS
authors: Shevkoplyas
mesh_terms: D001809:Blood Viscosity; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D011786:Quality Control; D015203:Reproducibility of Results