title: Chemical constituents from Sambucus adnata and their protein-tyrosine phosphatase 1B inhibitory activities.
abstract: The MeOH extract from the whole plants of Sambucus adnata has shown significant protein-tyrosine phosphatase 1B (PTP1B) inhibitory activity. Chemical study on the extract resulted in the isolation of thirteen compounds, including a novel triterpene (1). The structure of 1 was determined to be 1α,3β-dihydroxy-urs-12-en-11-one-3-yl palmitate on the basis of extensive spectroscopic analyses. Among the isolated compounds, ursolic acid, oleanolic acid and (±)-boehmenan showed the most potent PTP1B inhibitory activity in vitro with the IC(50) values of 4.1, 14.4 and 43.5 µm, respectively. The kinetic analysis indicated that (±)-boehmenan inhibits PTP1B activity in a competitive manner.
authors: Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274–8510, Japan.
authors: Tatsunori
authors: T
authors: Sasaki
authors: Wei
authors: W
authors: Li
authors: Haruna
authors: H
authors: Morimura
authors: Songpei
authors: S
authors: Li
authors: Qin
authors: Q
authors: Li
authors: Yoshihisa
authors: Y
authors: Asada
authors: Kazuo
authors: K
authors: Koike
mesh_terms: D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D027923:Sambucus / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C570313:1alpha,3beta-dihydroxy-urs-12-en-11-one-3-yl palmitate; D004791:Enzyme Inhibitors; D014315:Triterpenes; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1