title: Apricot carotenoids possess potent anti-amyloidogenic activity in vitro.
abstract: Alzheimer's disease (AD) is the most common form of dementia and is characterized by the progressive accumulation of amyloid β protein (Aβ) in areas of the brain. There has been an increased interest in screening for food-grade anti-amyloidogenic compounds in foodstuffs. The purpose of this study was to screen and identify bioactive compounds with anti-amyloidogenicity in apricot fruits using synthetic Aβ(1-42). The anti-amyloidogenicity was investigated using thioflavin T fluorescence assay, electron microscopy, and dot blotting analysis. The carotenoid fraction from apricot showed strong inhibitory effects against oligomer and fibril formation of Aβ and fibril-destabilizing effects. Among the peaks in the HPLC chromatogram, lutein showed the strongest inhibitory effect on Aβ fibril formation. The inhibitory effect was dependent on the number and portion of hydroxyl groups on both sides of carotenoids. These findings suggest that lutein in fruits may be useful as a preventive agent for amyloid-associated diseases.
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 minamiminowa, Ina, Nagano 399-4598, Japan.
authors: Shigeru
authors: S
authors: Katayama
authors: Hirofumi
authors: H
authors: Ogawa
authors: Soichiro
authors: S
authors: Nakamura
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000737:chemistry; D002338:Carotenoids / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D027861:Prunus / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D016229:Amyloid beta-Peptides; D002338:Carotenoids