title: Iron, copper, and zinc transport: inhibition of divalent metal transporter 1 (DMT1) and human copper transporter 1 (hCTR1) by shRNA.
abstract: Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms. They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and synaptic transmission. Because of their role in many critical functions, they are commonly used in food fortification and supplementation strategies globally. To determine the involvement of divalent metal transporter 1 (DMT1) and human copper transporter 1 (hCTR1) on Fe, Cu, and Zn uptake, Caco-2 cells were transfected with four different shRNA plasmids to selectively inhibit DMT1 or hCTR1 transporter expression. Fe and Cu uptake and total Zn content measurements were performed in shRNA-DMT1 and shRNA-hCTR1 cells. Both shRNA-DMT1 and shRNA-hCTR1 cells had lower apical Fe uptake (a decrease of 51% and 41%, respectively), Cu uptake (a decrease of 25.8% and 38.5%, respectively), and Zn content (a decrease of 23.1% and 22.7%, respectively) compared to control cells. These results confirm that DMT1 is involved in active transport of Fe, Cu, and Zn although Zn showed a different relative capacity. These results also show that hCTR1 is able to transport Fe and Zn.
authors: Micronutrient Laboratory, Institute of Nutrition and Food Technology, University of Chile, El Líbano, 5524 Macul, Santiago, Chile.
authors: Alejandra
authors: A
authors: Espinoza
authors: Solange
authors: S
authors: Le Blanc
authors: Manuel
authors: M
authors: Olivares
authors: Fernando
authors: F
authors: Pizarro
authors: Manuel
authors: M
authors: Ruz
authors: Miguel
authors: M
authors: Arredondo
mesh_terms: D001692:Biological Transport / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D018938:Caco-2 Cells; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003300:Copper / Q000378:metabolism* / Q000493:pharmacokinetics; D000079923:Copper Transporter 1; D056827:Endosomal Sorting Complexes Required for Transport; D006801:Humans; D007501:Iron / Q000378:metabolism* / Q000493:pharmacokinetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D015032:Zinc / Q000378:metabolism* / Q000493:pharmacokinetics; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D003300:Copper; D000079923:Copper Transporter 1; D056827:Endosomal Sorting Complexes Required for Transport; D007501:Iron; D034741:RNA, Small Interfering; D015032:Zinc; D000099236:Solute Carrier Family 11, Member 2; C503812:CHMP2B protein, human; C490079:SLC31A1 protein, human