title: Correspondence between salivary proteomic pattern and clinical course in primary Sjögren syndrome and non-Hodgkin's lymphoma: a case report.
abstract: BACKGROUND
  In the last years human proteomic has represented a promising tool to promote the communication between basic and clinical science.
  
  
  METHODS
  To explore the correspondence between salivary proteomic profile and clinical response, herein, we used a proteomic approach to analyse the whole saliva of a patient with primary Sjögren's Syndrome (pSS) and non-Hodgkin's-MALT type parotid lymphoma before, during and after a standard treatment with cyclophosphamide (CTX) and rituximab (RTX). To identify any discriminatory therapeutic salivary biomarker patient's whole saliva was collected at the baseline, after the fourth infusion of rituximab, and on remission and analysed combining two-dimensional electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry.
  
  
  RESULTS
  Proteomic results obtained from the comparison of salivary samples indicated several qualitative and quantitative modifications in the salivary expression of putative albumin, immunoglobulin J chain, Ig kappa chain C region, alpha-1-antitrypsin, haptoglobin and Ig alpha-1 chain C region.
  
  
  CONCLUSIONS
  This study suggests that clinical and functional changes of the salivary glands driven by autoimmune and lymphoproliferative processes might be reflected in patients' whole saliva proteins, shedding new light on the potential usefulness of salivary proteomic analysis in the identification of prognostic and therapeutic biomarkers for patients with pSS and non Hodgkin's lymphomas.
authors: Department of Internal Medicine, Rheumatology Unit, University of Pisa, Pisa, Italy.
authors: Chiara
authors: C
authors: Baldini
authors: Laura
authors: L
authors: Giusti
authors: Federica
authors: F
authors: Ciregia
authors: Ylenia
authors: Y
authors: Da Valle
authors: Camillo
authors: C
authors: Giacomelli
authors: Elena
authors: E
authors: Donadio
authors: Francesco
authors: F
authors: Ferro
authors: Sara
authors: S
authors: Galimberti
authors: Valentina
authors: V
authors: Donati
authors: Laura
authors: L
authors: Bazzichi
authors: Stefano
authors: S
authors: Bombardieri
authors: Antonio
authors: A
authors: Lucacchini
mesh_terms: D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D010306:Parotid Gland / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000378:metabolism* / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D012471:Salivary Proteins and Peptides