title: A combined array-based comparative genomic hybridization and functional library screening approach identifies mir-30d as an oncomir in cancer.
abstract: Oncomirs are microRNAs (miRNA) that acts as oncogenes or tumor suppressor genes. Efficient identification of oncomirs remains a challenge. Here we report a novel, clinically guided genetic screening approach for the identification of oncomirs, identifying mir-30d through this strategy. mir-30d regulates tumor cell proliferation, apoptosis, senescence, and migration. The chromosomal locus harboring mir-30d was amplified in more than 30% of multiple types of human solid tumors (n = 1,283). Importantly, higher levels of mir-30d expression were associated significantly with poor clinical outcomes in ovarian cancer patients (n = 330, P = 0.0016). Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3. The guided genetic screening approach validated by this study offers a powerful tool to identify oncomirs that may have utility as biomarkers or targets for drug development.
authors: Ovarian Cancer Research Center and Department of Obstetrics & Gynecology, University of Pennsylvania, Wistar Institute, Philadelphia, Pennsylvania, USA.
authors: Ning
authors: N
authors: Li
authors: Sippy
authors: S
authors: Kaur
authors: Joel
authors: J
authors: Greshock
authors: Heini
authors: H
authors: Lassus
authors: Xiaomin
authors: X
authors: Zhong
authors: Yanling
authors: Y
authors: Wang
authors: Arto
authors: A
authors: Leminen
authors: Zhongjun
authors: Z
authors: Shao
authors: Xiaowen
authors: X
authors: Hu
authors: Shun
authors: S
authors: Liang
authors: Dionyssios
authors: D
authors: Katsaros
authors: Qihong
authors: Q
authors: Huang
authors: Ralf
authors: R
authors: Bützow
authors: Barbara L
authors: BL
authors: Weber
authors: George
authors: G
authors: Coukos
authors: Lin
authors: L
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation; D002874:Chromosome Mapping; D055028:Comparative Genomic Hybridization; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs