title: Discovery and characterization of proteins associated with aflatoxin-resistance: evaluating their potential as breeding markers.
abstract: Host resistance has become a viable approach to eliminating aflatoxin contamination of maize since the discovery of several maize lines with natural resistance. However, to derive commercial benefit from this resistance and develop lines that can aid growers, markers need to be identified to facilitate the transfer of resistance into commercially useful genetic backgrounds without transfer of unwanted traits. To accomplish this, research efforts have focused on the identification of kernel resistance-associated proteins (RAPs) including the employment of comparative proteomics to investigate closely-related maize lines that vary in aflatoxin accumulation. RAPs have been identified and several further characterized through physiological and biochemical investigations to determine their causal role in resistance and, therefore, their suitability as breeding markers. Three RAPs, a 14 kDa trypsin inhibitor, pathogenesis-related protein 10 and glyoxalase I are being investigated using RNAi gene silencing and plant transformation. Several resistant lines have been subjected to QTL mapping to identify loci associated with the aflatoxin-resistance phenotype. Results of proteome and characterization studies are discussed.
authors: USDA-ARS, Southern Regional Research Center, New Orleans, LA 70124, USA. deepak.bhatnagar@ars.usda.gov
authors: Robert L
authors: RL
authors: Brown
authors: Zhi-Yuan
authors: ZY
authors: Chen
authors: Marilyn
authors: M
authors: Warburton
authors: Meng
authors: M
authors: Luo
authors: Abebe
authors: A
authors: Menkir
authors: Ahmad
authors: A
authors: Fakhoury
authors: Deepak
authors: D
authors: Bhatnagar
mesh_terms: D000348:Aflatoxins / Q000633:toxicity*; D015415:Biomarkers / Q000032:analysis; D001947:Breeding; D002874:Chromosome Mapping; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D040641:Quantitative Trait Loci; D003313:Zea mays / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000348:Aflatoxins; D015415:Biomarkers; D010940:Plant Proteins