title: Antigenic profile of Blomia tropicalis, Aleuroglyphus ovatus and Glycycometus malaysiensis.
abstract: House dust mites and storage mites are well-known causes for allergenic diseases.   The aim of this study was to investigate the immunogenic sites of Blomia tropicalis,   Aleurogyphus ovatus and Glycycometus malaysiensis. The mites were maintained in a   culture medium at 25ºC and 75% relative humidity. Mites were harvested either with heat   escape or floatation method, purified, homogenized, quantified and used for the production of   polyclonal antibody and immunostaining. For each species of mites, five male mice and five   male rats were randomly selected and immunized intraperitoneally with respective crude   mite extract at two-weekly intervals. Blomia tropicalis, A. ovatus or G. malaysiensis whole   mites and paraffin-embedded mite sections were immunostained with the respective polyclonal   antibody. The faecal pellets of mites were intensely stained for all the three species in the   present study. The legs of sectioned A. ovatus were not immunogenic as compared with   those of G. malaysiensis and B. tropicalis. The outer layer (cuticle) of whole mites and the   eggs for these species were very immunogenic. Hence, the polyclonal antibodies obtained in   this study may serve as potential tools in detecting the eggs and immature mites in   environmental samples. Future studies should focus on the antigenic components of eggs   since they were relatively abundant in dust and highly antigenic as seen in the present study.
authors: International Medical University, 57000 Kuala Lumpur, Malaysia.
authors: J C
authors: JC
authors: Tang
authors: S F
authors: SF
authors: Wong
authors: J W
authors: JW
authors: Mak
authors: T M
authors: TM
authors: Ho
mesh_terms: D000053:Acari / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D000941:Antigens