title: Photoactivatable caged cyclic RGD peptide for triggering integrin binding and cell adhesion to surfaces.
abstract: We report the synthesis and properties of a photoactivatable caged RGD peptide and its application for phototriggering integrin- and cell-binding to surfaces. We analysed in detail 1) the differences in the integrin-binding affinity of the caged and uncaged forms by quartz crystal microbalance (QCM) studies, 2) the efficiency and yield of the photolytic uncaging reaction, 3) the biocompatibility of the photolysis by-products and irradiation conditions, 4) the possibility of site, temporal and density control of integrin-binding and therefore human cell attachment, and 5) the possibility of in situ generation of cell patterns and cell gradients by controlling the UV exposure. These studies provide a clear picture of the potential and limitations of caged RGD for integrin-mediated cell adhesion and demonstrate the application of this approach to the control and study of cell interactions and responses.
authors: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
authors: Melanie
authors: M
authors: Wirkner
authors: Simone
authors: S
authors: Weis
authors: Verónica
authors: V
authors: San Miguel
authors: Marta
authors: M
authors: Álvarez
authors: Radu A
authors: RA
authors: Gropeanu
authors: Marcelo
authors: M
authors: Salierno
authors: Anne
authors: A
authors: Sartoris
authors: Ronald E
authors: RE
authors: Unger
authors: C James
authors: CJ
authors: Kirkpatrick
authors: Aránzazu
authors: A
authors: del Campo
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000378:metabolism*; D011485:Protein Binding; D058525:Quartz Crystal Microbalance Techniques; D014466:Ultraviolet Rays
chemical_list: D016023:Integrins; D010456:Peptides, Cyclic; C507169:cyclic arginine-glycine-aspartic acid peptide