title: Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray.
abstract: Widespread bacterial resistance to antibiotics is a significant public health concern. To remain a step ahead of evolving bacteria, new methods to study resistance to antibacterials and to uncover novel antibiotics that evade resistance are urgently needed. Herein, microarray-based methods that have been developed to study aminoglycoside modification by resistance-causing enzymes are reviewed. These arrays can also be used to study the binding of aminoglycoside antibiotics to a mimic of their therapeutic target, the rRNA aminoacyl site (A-site), and how modification by resistance-causing enzymes affects their abilities to bind RNA.
authors: Department of Chemistry, The Scripps Research Institute, Scripps Florida, Jupiter, FL, USA. mddisney@buffalo.edu
authors: Matthew D
authors: MD
authors: Disney
mesh_terms: D000617:Aminoglycosides / Q000737:chemistry / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D046228:Microarray Analysis / Q000379:methods*; D014166:Transferases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014166:Transferases