title: Mushroom tyrosinase inhibitors from mung bean (Vigna radiatae L.) extracts.
abstract: A seventy percent ethanol from mung bean (Vigna radiatae L.) was extracted further with CH(2)Cl(2), EtOAc and n-BuOH to afford four fractions: CH(2)Cl(2)-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. When using l-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase, the EtOAc-soluble fractions showed the highest inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guided fractionation method) from the EtOAc-soluble fractions. Vitexin and isovitexin showed high inhibitory activities, with IC(50) values of 6.3 and 5.6 mg/ml, respectively. This is the first study on the active compositions of azuki beans against mushroom tyrosinase.
authors: Institute of Crop Science, Chinese Academy of Agricultural Sciences, No. 80 South Xueyuan Road, Haidian District, Beijing 100081, People's Republic of China.
authors: Yang
authors: Y
authors: Yao
authors: Xuzhen
authors: X
authors: Cheng
authors: Lixia
authors: L
authors: Wang
authors: Suhua
authors: S
authors: Wang
authors: Guixing
authors: G
authors: Ren
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D007887:Fabaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D014442:Monophenol Monooxygenase