title: Antitumor activity of peptide amphiphile nanofiber-encapsulated camptothecin.
abstract: Self-assembling peptide amphiphile (PA) nanofibers were used to encapsulate camptothecin (CPT), a naturally occurring hydrophobic chemotherapy agent, using a solvent evaporation technique. Encapsulation by PA nanofibers was found to improve the aqueous solubility of the CPT molecule by more than 50-fold. PAs self-assembled into nanofibers in the presence of CPT as demonstrated by transmission electron microscopy. Small-angle X-ray scattering results suggest a slight increase in diameter of the nanofiber to accommodate the hydrophobic cargo. In vitro studies using human breast cancer cells show an enhancement in antitumor activity of the CPT when encapsulated by the PA nanofibers. In addition, using a mouse orthotopic model of human breast cancer, treatment with PA nanofiber-encapsulated CPT inhibited tumor growth. These results highlight the potential of this model PA system to be adapted for delivery of hydrophobic therapies to treat a variety of diseases including cancer.
authors: Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, Illinois 60611, USA.
authors: Stephen
authors: S
authors: Soukasene
authors: Daniel J
authors: DJ
authors: Toft
authors: Tyson J
authors: TJ
authors: Moyer
authors: Hsuming
authors: H
authors: Lu
authors: Hyung-Kun
authors: HK
authors: Lee
authors: Stephany M
authors: SM
authors: Standley
authors: Vincent L
authors: VL
authors: Cryns
authors: Samuel I
authors: SI
authors: Stupp
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002166:Camptothecin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D053769:Nanocapsules; D010455:Peptides; D012997:Solvents; D002166:Camptothecin