title: Effect of RNA interference of BID and BAX mRNAs on apoptosis in granulosa cell-derived KGN cells.
abstract: In mitochondrion-dependent type II apoptosis, BH3-interacting domain death agonist (BID) and BCL-2-associated X protein (BAX) promote death ligand and receptor-mediated cell death. In porcine ovaries, the levels of BID and BAX increase in follicular granulosa cells during atresia. In the present study, to confirm the pro-apoptotic activity of BID and BAX in granulosa cells, we examined the effect of RNA interference of BID or BAX on apoptosis using a human ovarian granulosa tumor cell line, KGN. By reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, expression of BID and BAX was detected in KGN cells. Then, we suppressed BID and BAX mRNA expression in KGN cells using small interfering RNA (siRNA). When BID or BAX was suppressed, a significant decrease in the apoptotic cell rate was noted. In granulosa-derived cells, BID and BAX showed pro-apoptotic activity. These results suggest that BID and BAX act as signal-transducing factors in mitochondrion-dependent type II apoptosis.
authors: Animal Resource Science Center, The University of Tokyo, Kasama 319-0206, Japan.
authors: Takafumi
authors: T
authors: Sai
authors: Fuko
authors: F
authors: Matsuda
authors: Yasufumi
authors: Y
authors: Goto
authors: Akihisa
authors: A
authors: Maeda
authors: Miki
authors: M
authors: Sugimoto
authors: Hong-Mei
authors: HM
authors: Gao
authors: Abul Khair Mohammad Ahan
authors: AK
authors: Kabir
authors: Jun-You
authors: JY
authors: Li
authors: Noboru
authors: N
authors: Manabe
mesh_terms: D017209:Apoptosis; D051023:BH3 Interacting Domain Death Agonist Protein / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D034622:RNA Interference; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis*
chemical_list: C494826:BAX protein, human; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D051028:bcl-2-Associated X Protein