title: Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation.
abstract: Dynamics of cold tolerance and crown proteome composition has been analysed in a set of two winter wheat cultivars Mironovskaya 808 and Bezostaya 1 and four reciprocal substitution lines with interchanged chromosomes 5A and 5B during a long-term cold-acclimation (CA) treatment. Proteome analysis has revealed 298 differently abundant spots during experiment. Most of them (260) were changed due to CA process and only 52 spots displayed differences between genotypes. Two hundred and seven protein spots were successfully identified by tandem mass spectrometry. Comparison of samples before and after vernalization fulfillment by a combination of ANOVA and Student' T-test displayed ten differentially abundant protein spots (e.g. chopper chaperones). However, differences in the accumulation of these spots did not reflect differences in vernalization requirement of genotypes. Therefore, our results indicate that vernalization process has not influenced total proteome of CA wheat crowns. A few protein spots (14 spots; e.g. malate dehydrogenase) revealed differential accumulation levels between the individual genotypes or their groups possessing chromosome 5A or 5B from Mironovskaya 808 versus Bezostaya 1. The study has shown the effect of chromosome 5A on physiological traits and also proteome in winter wheat. Putative candidate protein markers for cold tolerance in wheat are discussed.
authors: Crop Research Institute, Department of Genetics and Plant Breeding, Prague, Czech Republic. vitamvas@vurv.cz
authors: Pavel
authors: P
authors: Vítámvás
authors: Ilja T
authors: IT
authors: Prášil
authors: Klára
authors: K
authors: Kosová
authors: Sébastien
authors: S
authors: Planchon
authors: Jenny
authors: J
authors: Renaut
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D032461:Chromosomes, Plant; D016000:Cluster Analysis; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D025341:Principal Component Analysis; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D010940:Plant Proteins; D020543:Proteome