title: Development of microsatellite markers in Actinidia arguta (Actinidiaceae) based on the NCBI data platform.
abstract: OBJECTIVE
  Expressed sequence tag (EST)-derived microsatellite markers in Actinidia arguta were developed for genotyping and genetic mapping.
  
  
  RESULTS
  One hundred and fifty EST-simple sequence repeat (SSR) primer pairs were obtained from the National Center for Biotechnology Information (NCBI) A. chinensis database using SSR Hunter 1.3. With the other 20 reported primers, 170 primer pairs were screened using 16 samples. A total of 72 primers pairs were successively developed for A. arguta. Fifteen of the developed markers were characterized in A. arguta populations from Changbai Mountain and Daba Mountain. The mean number of alleles per locus in the Changbai and Daba populations was 5.133 and 4.133, respectively.
  
  
  CONCLUSIONS
  Development of Actinidia ESTs from the NCBI database is an effective method of obtaining SSR markers for A. arguta. These markers will be useful for genome mapping and molecular breeding in A. arguta.
authors: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors: Yuping
authors: Y
authors: Man
authors: Yanchang
authors: Y
authors: Wang
authors: Lei
authors: L
authors: Zhang
authors: Zuozhou
authors: Z
authors: Li
authors: Rui
authors: R
authors: Qin
authors: Zhengwang
authors: Z
authors: Jiang
authors: Xiaorong
authors: X
authors: Sun
authors: Changjiang
authors: C
authors: Liu
mesh_terms: D029042:Actinidia / Q000235:genetics*; D002681:China; D002874:Chromosome Mapping; D017931:DNA Primers; D018744:DNA, Plant; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D005828:Genetics, Population; D005843:Geography; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers