title: Human leukocyte antigen-G 3' untranslated region polymorphisms are associated with better kidney allograft acceptance.
abstract: Human leukocyte antigen-G (HLA-G) plays a well-recognized role in the modulation of the immune response, and HLA-G expression has been associated with increased graft survival and decreased rejection episodes. To investigate the role of the HLA-G 3' untranslated region (3'UTR) in renal transplantation, we evaluated several polymorphic sites (14-bp Del/Ins +3003T/C, +3010C/G, +3027C/A, +3035C/T, +3142G/C, and +3187A/G) in patients exhibiting or not exhibiting rejection episodes. A total of 104 patients (15 with acute and 48 with chronic rejection, and 41 with no rejection) and 142 healthy individuals were studied. HLA-G 3'UTR was typed by direct sequencing. The +3035C-C genotype was more frequent in patients exhibiting chronic rejection compared with healthy controls, and the +3035C-T genotype was less frequent in chronic rejection compared with patients without rejection (acute plus chronic) or compared with healthy controls. The +3187G-A genotype, in which the A allele is associated with increased mRNA degradation, showed increased frequency in the rejection group (acute plus chronic) when compared with healthy controls. The 14 base pair Deletion/Insertion genotype was marginally increased in patients with acute rejection. This is the first study to show associations among numerous polymorphic sites in the HLA-G 3'UTR in kidney allotransplantation, which may contribute to the understanding of HLA-G post-transcriptional mechanisms.
authors: Program of Basic and Applied Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. daianicristina@yahoo.com.br
authors: Daiani Cristina
authors: DC
authors: Cilião Alves
authors: Janaina Cristiana
authors: JC
authors: de Oliveira Crispim
authors: Erick C
authors: EC
authors: Castelli
authors: Celso Teixeira
authors: CT
authors: Mendes-Junior
authors: Neifi Hassan Saloun
authors: NH
authors: Deghaide
authors: Gyl Eanes
authors: GE
authors: Barros Silva
authors: Roberto Silva
authors: RS
authors: Costa
authors: Luciana Tanajura
authors: LT
authors: Saber
authors: Philippe
authors: P
authors: Moreau
authors: Eduardo Antonio
authors: EA
authors: Donadi
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D006085:Graft Survival / Q000235:genetics / Q000276:immunology; D059951:HLA-G Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology* / Q000379:methods; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D059951:HLA-G Antigens