title: Effect of milk renewal on human periodontal ligament fibroblast viability in vitro.
abstract: Milk has been studied extensively and has gained wide acceptance as a suitable storage medium capable of maintenance of avulsed teeth that cannot be replanted immediately. The objective of this study was to evaluate whether the renewal of milk as a storage medium every 24 h for up to 120 h is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability in vitro. Plates with confluent PDLF were soaked in minimum essential medium (MEM) at 37°C (positive control) and in skimmed milk (22 wells) and water (negative control) for 24, 48, 72, 96, and 120 h at 5 and 20°C. The skimmed milk was renewed every 24 h in 11 of the wells of each plate. After these periods, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis and Scheffé tests (α = 5%). At 24 h, milk and MEM performed similarly. However, from 48 h onwards, MEM was significantly better than renewed and not renewed milk at both temperatures. Regardless of temperature (5 or 20°C), renewal of milk with fresh milk did not affect its ability to maintain PDLF viability.
authors: Department of Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
authors: Beatriz D M
authors: BD
authors: de Souza
authors: Débora D
authors: DD
authors: Lückemeyer
authors: Wilson T
authors: WT
authors: Felippe
authors: Ana M H
authors: AM
authors: Alves
authors: Cláudia M O
authors: CM
authors: Simões
authors: Mara C S
authors: MC
authors: Felippe
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D005347:Fibroblasts / Q000737:chemistry*; D006801:Humans; D008892:Milk / Q000737:chemistry*; D010513:Periodontal Ligament / Q000737:chemistry*; D013696:Temperature; D013778:Tetrazolium Salts; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D003470:Culture Media; D013778:Tetrazolium Salts