title: Hydrogels as intracellular depots for drug delivery.
abstract: The intracellular activity and drug depot characteristics of micrometer-sized hydrogels are described. The hydrogel structure is formed after cellular uptake of a solid polymeric nanoparticle that swells in response to mildly acidic conditions as it transforms from a hydrophobic to a hydrophilic structure. These nanoparticles are rapidly taken up into A549 human non-small cell lung cancer cells with 88.3 ± 0.8% of cells experiencing uptake in 24 h, undergo expansion to release encapsulated drug and can effectively deliver chemotherapeutics in vitro. The anticancer drug paclitaxel was also shown to have a 3- to 4-fold increased affinity for the expanded nanoparticle state, allowing the expansile nanoparticles to act as intracellular drug depots and concentrate the drug locally.
authors: Departments of Biomedical Engineering and Chemistry, Boston University, Boston, Massachusetts 02215, United States.
authors: Kimberly Ann V
authors: KA
authors: Zubris
authors: Yolonda L
authors: YL
authors: Colson
authors: Mark W
authors: MW
authors: Grinstaff
mesh_terms: D001692:Biological Transport; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004339:Drug Compounding; D006801:Humans; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009994:Osmolar Concentration; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D011109:Polymethacrylic Acids; D017239:Paclitaxel