title: A revertible, autonomous, self-assembled DNA-origami nanoactuator.
abstract: A DNA-origami actuator capable of autonomous internal motion in accord to an external chemical signal was designed, built, operated and imaged. The functional DNA nanostructure consists of a disk connected to an external ring in two, diametrically opposite points. A single stranded DNA, named probe, was connected to two edges of the disk perpendicularly to the axis of constrain. In the presence of a hybridizing target molecule, the probe coiled into a double helix that stretched the inner disk forcing the edges to move toward each other. The addition of a third single stranded molecule that displaced the target from the probe restored the initial state of the origami. Operation, dimension and shape were carefully characterized by combining microscopy and fluorescence techniques.
authors: Dipartimento di Scienze e Agrarie ed Ambientali, Università degli Studi di Udine, via delle Scienze 208, 33100 Udine, Italy.
authors: Monica
authors: M
authors: Marini
authors: Luca
authors: L
authors: Piantanida
authors: Rita
authors: R
authors: Musetti
authors: Alpan
authors: A
authors: Bek
authors: Mingdong
authors: M
authors: Dong
authors: Flemming
authors: F
authors: Besenbacher
authors: Marco
authors: M
authors: Lazzarino
authors: Giuseppe
authors: G
authors: Firrao
mesh_terms: D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D018625:Microscopy, Atomic Force; D009038:Motion; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation
chemical_list: D004277:DNA, Single-Stranded; D004247:DNA