title: [Determination of four alkaloids in Corydalis decumbens by HPLC].
abstract: OBJECTIVE
  To establish a quantitative HPLC method for determination of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine, in Corydalis decumbens.
  
  
  METHODS
  The separation was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) at a flow rate of 1.0 mL x min(-1) using mixtures of two solvents [A(20 mmol x L(-1) ammonium acetate)-B(acetonitrile)]: with a gradient elution. The column oven temperature was 30 degrees C and the detection wavelength was set at 280 nm.
  
  
  RESULTS
  The 4 alkaloids were well separated by this HPLC method. Linearifies of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine were good in the ranges of 1.44-46.0 (r = 0.999 4), 1.2640.2 (r = 0.999 8), 1.37-44.0 (r = 0.999 9), and 1.3643.6 mg x L(-1) (r = 0.999 9), respectively. The average recoveries were 98.2% with RSD 2.7% for protopine, 101.9% with RSD 2.5% for palmatine hydrochloride, 102.8% with RSD 3.5% for tetrahydropalmatine, and 98.8% with RSD 3.1% for tetrahydropalmatine.
  
  
  CONCLUSIONS
  This method is proved to be convenient, reliable and accurate., and it can be used for quality control of C. decumbens.
authors: College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China. shenyan@wzu.edu.cn
authors: Yan
authors: Y
authors: Shen
authors: Chao
authors: C
authors: Han
authors: Biqi
authors: B
authors: Xia
authors: Yongfang
authors: Y
authors: Zhou
authors: Cuiping
authors: C
authors: Liu
authors: Aili
authors: A
authors: Liu
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031311:Corydalis / Q000737:chemistry*; D004355:Drug Stability; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids