title: Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification.
abstract: A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations.
authors: National Foot-and-Mouth Disease Reference Laboratory of China, Key laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, P.R. China.
authors: Hao-tai
authors: HT
authors: Chen
authors: Jie
authors: J
authors: Zhang
authors: Yong-sheng
authors: YS
authors: Liu
authors: Xiang-tao
authors: XT
authors: Liu
mesh_terms: D000818:Animals; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification* / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D058889:Molecular Typing / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D012367:RNA, Viral