title: Effect of the lectin of Bauhinia variegata and its recombinant isoform on surgically induced skin wounds in a murine model.
abstract: Lectins are a structurally heterogeneous group of highly specific carbohydrate-binding proteins. Due to their great biotechnological potential, lectins are widely used in biomedical research. The purpose of the present study was to evaluate the healing potential of the lectin of Bauhinia variegata (nBVL) and its recombinant isoform (rBVL-1). Following surgical creation of dorsal skin wounds, seven groups of mice were submitted to topical treatment for 12 days with lectin, D-galactose, BSA and saline. The animals were anesthetized and euthanized on POD 2, 7 and 12 in order to evaluate the healing potential of each treatment. The parameters considered included wound size, contraction rate, epithelialization rate and histopathological findings. Wound closure was fastest in animals treated with rBVL-1 (POD 7). nBVL was more effective than the controls. All skin layers were reconstructed and keratin deposition increased. Our findings indicate that the lectin of Bauhinia variegata possesses pro-healing properties and may be employed in the treatment of acute skin wounds.
authors: Integrated Laboratory of Biomolecules (LIBS), School of Medicine of the Federal University of Ceará, Sobral, Brazil. ziullec@gmail.com
authors: Luiz Gonzaga do Nascimento
authors: LG
authors: Neto
authors: Luciano da Silva
authors: Lda S
authors: Pinto
authors: Rafaela Mesquita
authors: RM
authors: Bastos
authors: Francisco Flávio Vasconcelos
authors: FF
authors: Evaristo
authors: Mayron Alves de
authors: MA
authors: Vasconcelos
authors: Victor Alves
authors: VA
authors: Carneiro
authors: Francisco Vassiliepe Sousa
authors: FV
authors: Arruda
authors: Ana Lúcia Figueiredo
authors: AL
authors: Porto
authors: Rodrigo Bainy
authors: RB
authors: Leal
authors: Valdemiro Amaro da Silva
authors: VA
authors: Júnior
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: Edson Holanda
authors: EH
authors: Teixeira
mesh_terms: D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D062109:Dermatologic Surgical Procedures; D008297:Male; D051379:Mice; D037121:Plant Lectins / Q000235:genetics / Q000494:pharmacology*; D020033:Protein Isoforms / Q000235:genetics / Q000494:pharmacology*; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D037121:Plant Lectins; D020033:Protein Isoforms; D011994:Recombinant Proteins