title: Ascorbic acid ameliorates oxidative damage induced by maternal low-level lead exposure in the hippocampus of rat pups during gestation and lactation.
abstract: This study was to investigate the effects of ascorbic acid on the hippocampus of suckling rats in the presence of lead (Pb)-induced oxidative stress. Pregnant Sprague-Dawley rats received treatment with drinking water, divided into three groups, as follows: (1) distilled water; (2) 0.2% Pb; (3) 0.2% Pb+ascorbic acid (100mg/kg/day). Rat pups were euthanized at the age of 21days and their brain tissue was examined using light microscopy. Protein levels of Cu/Zn superoxide dismutase (Cu/Zn SOD), manganese superoxide dismutase (Mn SOD), and catalase (CAT) in the hippocampus were determined by Western blotting. We found a significant decrease in levels of Cu/Zn SOD and Mn SOD among Pb-exposed pups. Ascorbic acid supplementation appeared to negate the decrease in protein levels for Cu/Zn SOD and Mn SOD. In the case of CAT, there was no effect from Pb administration alone and Pb plus ascorbic acid appeared to increase the levels. In histopathology, ascorbic acid decreased the number of damaged cells in cornu ammonis areas CA1, CA3, and the dentate gyrus (DG) in hippocampus. Our results showed that administration of ascorbic acid during pregnancy and lactation could ameliorate some of the oxidative damage induced by Pb exposure in the developing rat hippocampus.
authors: Department of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Byung-Joon
authors: BJ
authors: Chang
authors: Bong-Jun
authors: BJ
authors: Jang
authors: Tae Gen
authors: TG
authors: Son
authors: Ik-Hyun
authors: IH
authors: Cho
authors: Fu-Shi
authors: FS
authors: Quan
authors: Nong-Hoon
authors: NH
authors: Choe
authors: Sang-Soep
authors: SS
authors: Nahm
authors: Jong-Hwan
authors: JH
authors: Lee
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D007774:Lactation; D007854:Lead / Q000633:toxicity*; D018811:Maternal Exposure; D009474:Neurons / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D007854:Lead; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid