title: In vitro and in vivo effects of ketamine on generation and function of dendritic cells.
abstract: The question about how intravenous anesthetic reagents affect the development and function of dendritic cell subsets still has no comprehensive answers. Bone marrow cells differentiated with FMS-like tyrosine kinase 3 ligand in vitro represented the steady-state dendritic cell subsets. The effects of ketamine on the generation and function of dendritic cell subsets were investigated. We found that dendritic cell subsets responded to the anesthetic reagent ketamine in several aspects: 1) The in vitro and in vivo development of plasmacytoid dendritic cells were inhibited by ketamine at high concentrations; 2) The endocytosis of dendritic cells were not influenced by ketamine at concentrations from 50 - 200 µM; 3) The maturation markers of conventional dendritic cells were not changed by ketamine upon LPS or CpG stimulation, although the cytokines mRNA profiles were affected; 4) The allogenic-stimulatory activity of dendritic cells was suppressed by ketamine. In conclusion, ketamine hampered plasmacytoid dendritic cell subset development both in vivo and in vitro. The dendritic cells maturation and downstream responses towards different toll-like receptor stimuli were differently regulated by ketamine treatment.
authors: Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Xi Road, Yuexiu District, 510210 Guangzhou, China. jingxian.zeng@yahoo.com
authors: Jingxian
authors: J
authors: Zeng
authors: Shuxuan
authors: S
authors: Xia
authors: Wa
authors: W
authors: Zhong
authors: Jie
authors: J
authors: Li
authors: Liling
authors: L
authors: Lin
mesh_terms: D000778:Anesthetics, Dissociative / Q000494:pharmacology*; D000818:Animals; D000941:Antigens / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D004705:Endocytosis / Q000187:drug effects; D007649:Ketamine / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology; D051941:fms-Like Tyrosine Kinase 3 / Q000494:pharmacology
chemical_list: D000778:Anesthetics, Dissociative; D000941:Antigens; D016207:Cytokines; D051193:Toll-Like Receptors; D007649:Ketamine; C497971:Flt3 protein, mouse; D051941:fms-Like Tyrosine Kinase 3