title: Effects of water temperature increase and heavy metals contamination on WAP65 gene expression in sea bass (Dicentrarchus labrax) liver.
abstract: It has been previously demonstrated that "Warm temperature Acclimation-related 65 kD Protein" (WAP65) is involved in temperature acclimation, response to intoxication and infection, as well as in development. The expression of wap65-1 was investigated in the liver of European sea bass (Dicentrarchus labrax) during exposure to the increased temperature (from 12 deg C to 30 deg C) and during intoxication with four heavy metals: lead, cadmium, copper and zinc. Post temperature increase wap65 expression was highest after one hour at 30 deg C. After 1 to 4 weeks at 30 deg C wap65 transcript levels did not differ from the 12 deg C control group, similar to observations regarding the heat shock protein, hsp70. Upregulation of wap65 was detected after treatment (intoxication) with cadmium (0.5 μg/l). In contrast, a slight, but significant down regulation of wap65 was seen after copper (5 μg/l) intoxication. These data indicate that functional analyses of WAP65 are needed to understand the differential regulation of this gene by metals. The role of WAP65 may be similar to that of HSP70, which has generalized functions in responding to certain stressors and maintaining normal cell physiology.
authors: Équipe de Biologie Moléculaire Marine-PROTEE, Université du Sud Toulon-Var, BP 20132, Avenue de l'Université, 83957 La Garde Cedex, France.
authors: S
authors: S
authors: Pierre
authors: K
authors: K
authors: Tarnowska
authors: L
authors: L
authors: Hachfi
authors: S
authors: S
authors: Coupé
authors: R
authors: R
authors: Simide
authors: S
authors: S
authors: Couvray
authors: C
authors: C
authors: Garnier
authors: M
authors: M
authors: Grimaldi
authors: S
authors: S
authors: Richard
authors: S
authors: S
authors: Gaillard
authors: N
authors: N
authors: Prévot-D'Alvise
authors: J P
authors: JP
authors: Grillasca
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism*; D019216:Metals, Heavy / Q000378:metabolism*; D013696:Temperature
chemical_list: D029941:Fish Proteins; D019216:Metals, Heavy