title: Modification of an acetone-sodium dodecyl sulfate disruption method for cellular protein extraction from neurotoxigenic Clostridium botulinum.
abstract: An acetone-sodium dodecyl sulfate disruption method was used for the extraction of cellular proteins from neurotoxigenic Clostridium botulinum. The amount of protein extracted per gram of dry weight and the protein profile as revealed by polyacrylamide gel electrophoresis was comparable to the extracts prepared by established methods, namely, sonication or agitation with beads. This method allows safer handling of C. botulinum by avoiding mechanical disruption, the generation of aerosols, and contamination of the apparatus.
authors: Molecular Characterization of Foodborne Pathogens Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA. saumya.bhaduri@.ars.usda.gov
authors: Saumya
authors: S
authors: Bhaduri
mesh_terms: D000096:Acetone; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification*; D003014:Clostridium botulinum / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D012967:Sodium Dodecyl Sulfate
chemical_list: D001426:Bacterial Proteins; D000096:Acetone; D012967:Sodium Dodecyl Sulfate