title: Investigation of biomarkers of bile tolerance in Lactobacillus casei using comparative proteomics.
abstract: The identification of cell determinants involved in probiotic features is a challenge in current probiotic research. In this work, markers of bile tolerance in Lactobacillus casei were investigated using comparative proteomics. Six L. casei strains were classified on the basis of their ability to grow in the presence of bile salts in vitro. Constitutive differences between whole cell proteomes of the most tolerant strain (L. casei Rosell-215), the most sensitive one (L. casei ATCC 334), and a moderately tolerant strain (L. casei DN-114 001) were investigated. The ascertained subproteome was further studied for the six strains in both standard and bile stressing conditions. Focus was on proteins whose expression levels were correlated with observed levels of bile tolerance in vitro, particularly those previously reported to be involved in the bile tolerance process of lactobacilli. Analysis revealed that 12 proteins involved in membrane modification (NagA, NagB, and RmlC), cell protection and detoxification (ClpL and OpuA), as well as central metabolism (Eno, GndA, Pgm, Pta, Pyk, Rp1l, and ThRS) were likely to be key determinants of bile tolerance in L. casei and may serve as potential biomarkers for phenotyping or screening purposes. The approach used enabled the correlation of expression levels of particular proteins with a specific probiotic trait.
authors: Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, 67400, Illkirch-Graffenstaden, France.
authors: Erwann
authors: E
authors: Hamon
authors: Peter
authors: P
authors: Horvatovich
authors: Magali
authors: M
authors: Bisch
authors: Françoise
authors: F
authors: Bringel
authors: Eric
authors: E
authors: Marchioni
authors: Dalal
authors: D
authors: Aoudé-Werner
authors: Saïd
authors: S
authors: Ennahar
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D016000:Cluster Analysis; D015870:Gene Expression; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D019936:Probiotics; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D018709:Statistics, Nonparametric; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D015415:Biomarkers; D020543:Proteome