title: Imaging of RNA in live cells.
abstract: Fluorescence microscopy and molecular tagging technologies have ushered in a new era in our understanding of protein localization and function in cells. This review summarizes recent efforts to extend some of these methods (and to create new ones) to imaging of RNA in live cells. Both fluorescent proteins and hybridization probes allow noncovalent labeling of specific RNA molecules with fluorescent dyes that allow detection and tracking in real time.
authors: Department of Chemistry, Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA. army@cmu.edu
authors: Bruce A
authors: BA
authors: Armitage
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012313:RNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D016601:RNA-Binding Proteins; D049452:Green Fluorescent Proteins; D012313:RNA