title: Proteomic study of malignant pleural mesothelioma by laser microdissection and two-dimensional difference gel electrophoresis identified cathepsin D as a novel candidate for a differential diagnosis biomarker.
abstract: To investigate the proteomic background of malignancies of the pleura, we examined and compared the proteomic profile of malignant pleural mesothelioma (MPM)(10 cases), lung adenocarcinoma (11 cases), squamous cell carcinoma of the lung (13 cases), pleomorphic carcinoma of the lung (3 cases) and synovial sarcoma (6 cases). Cellular proteins were extracted from specific populations of tumor cells recovered by laser microdissection. The extracted proteins were labeled with CyDye DIGE Fluor saturation dyes and subjected to two-dimensional difference gel electrophoresis (2D-DIGE) using a large format electrophoresis device. Among 3875 protein spots observed, the intensity of 332 was significantly different (Wilcoxon p value less than 0.05) and with more than two-fold inter-sample-group average difference between the different histology groups. Among these 332, 282 were annotated by LC-MS/MS and included known biomarker proteins for MPM, such as calretinin, as well as proteins previously uncharacterized in MPM. Tissue microarray immunohistochemistry revealed that the expression of cathepsin D was lower in MPM than in lung adenocarcinoma (15% vs. 44% of cases respectively in immunohistochemistry). In conclusion, we examined the protein expression profile of MPM and other lung malignancies, and identified cathepsin D to distinguish MPM from most popular lung cancer such as lung adenocarcinoma.
authors: Division of Pharmacoproteomics, National Cancer Center Research Institute, Japan.
authors: Mutsumi
authors: M
authors: Hosako
authors: Taika
authors: T
authors: Muto
authors: Yukiko
authors: Y
authors: Nakamura
authors: Koji
authors: K
authors: Tsuta
authors: Naobumi
authors: N
authors: Tochigi
authors: Hitoshi
authors: H
authors: Tsuda
authors: Hisao
authors: H
authors: Asamura
authors: Takeshi
authors: T
authors: Tomonaga
authors: Akira
authors: A
authors: Kawai
authors: Tadashi
authors: T
authors: Kondo
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D061346:Laser Capture Microdissection; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism*; D010997:Pleural Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins