title: Development of cathepsin-L cysteine proteinase based Dot-enzyme-linked immunosorbent assay for the diagnosis of Fasciola gigantica infection in buffaloes.
abstract: Native cathepsin-L cysteine proteinase (28 kDa) was purified from the excretory secretory products of Fasciola gigantica and was used for sero-diagnosis of F. gigantica infection in buffaloes by Dot-enzyme-linked immunosorbent assay (Dot-ELISA). The test detected F. gigantica field infection in these animals with a sensitivity of ∼ 90%. No specific IgG antibody binding was displayed by sera obtained from 76 buffaloes considered to be Fasciola and other parasite-free by microscopic examination of faeces and necropsy examination of liver, rumen and intestine. Additionally, sera from 156 Fasciola-free buffaloes, yet infected with Gigantocotyle explanatum, Paramphistomum epiclitum, Gastrothylax spp., Strongyloides papillosus and hydatid cyst were all negative, indicating that F. gigantica cathepsin-L cysteine proteinase does not cross-react with these helminth parasites in natural infection of the host. The data indicated that cathepsin-L cysteine proteinase based Dot-ELISA reached ∼ 90% sensitivity and 100% specificity with relation to above parasites in the detection of bubaline fasciolosis. The present Dot-ELISA diagnostic assay is relevant to the field diagnosis of F. gigantica infection in buffaloes.
authors: Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors: Anju
authors: A
authors: Varghese
authors: O K
authors: OK
authors: Raina
authors: Gaurav
authors: G
authors: Nagar
authors: Rajat
authors: R
authors: Garg
authors: P S
authors: PS
authors: Banerjee
authors: B R
authors: BR
authors: Maharana
authors: Justin D
authors: JD
authors: Kollannur
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification; D002020:Buffaloes; D002403:Cathepsins / Q000276:immunology* / Q000302:isolation & purification; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D057056:Cysteine Proteases / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D005209:Fasciola / Q000201:enzymology / Q000302:isolation & purification*; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D002403:Cathepsins; D057056:Cysteine Proteases; C086102:cat-L1B protein, Fasciola gigantica; D003546:Cysteine Endopeptidases