title: A sensitive liquid chromatography coupled with mass spectrometry method for the intracellular and plasma quantification of raltegravir after solid-phase extraction.
abstract: BACKGROUND
  Liquid chromatography coupled with mass spectrometry for the quantification of raltegravir in human plasma and peripheral blood mononuclear cells has been developed.
  
  
  METHODS
  Sample preparations were based on a fully automated solid-phase extraction process. Mass spectrometric data were acquired in a single-ion monitoring method. Raltegravir and quinoxaline, the internal standard, were well separated in a gradient mode over 15 min.
  
  
  RESULTS
  Validation study exhibited excellent linearity, with good intra- and inter-day precision and accuracy.
  
  
  CONCLUSIONS
  The assay was successfully applied to the raltegravir quantification in HIV-infected patients.
authors: Department of Clinical Pharmacokinetics EA 2968, Clinical Pharmacy Haut-Lévêque Hospital CHU Bordeaux, University of Bordeaux 2, Pessac, France.
authors: Stéphanie
authors: S
authors: Mosnier-Thoumas
authors: Sarah
authors: S
authors: Djabarouti
authors: Fabien
authors: F
authors: Xuereb
authors: Estibaliz
authors: E
authors: Lazaro
authors: Jean-Luc
authors: JL
authors: Pellegrin
authors: Marie-Claude
authors: MC
authors: Saux
authors: Dominique
authors: D
authors: Breilh
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000032:analysis* / Q000097:blood; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D011760:Pyrrolidinones / Q000032:analysis* / Q000097:blood; D011786:Quality Control; D011810:Quinoxalines / Q000032:analysis; D000068898:Raltegravir Potassium; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D019380:Anti-HIV Agents; D007202:Indicators and Reagents; D011760:Pyrrolidinones; D011810:Quinoxalines; D000068898:Raltegravir Potassium