title: Electrocatalytic reaction of hydrogen peroxide and NADH based on poly(neutral red) and FAD hybrid film.
abstract: A simple method to immobilize poly(neutral red) (PNR) and flavin adenine dinucleotide (FAD) hybrid film (PNR/FAD) by cyclic voltammetry is proposed. The PNR/FAD hybrid film can be easily prepared on an electrode surface involving electropolymerization of neutral red (NR) monomers and the electrostatic interaction between the positively charged PNR and the negatively charged FAD. It exhibits electroactive, stable, surface-confined, pH-dependent, nano-sized, and compatible properties. It provides good electrocatalytic properties to various species. It shows a sensitivity of 5.4 μA mM(-1) cm(-2) and 21.5 μA mM(-1) cm(-2) for hydrogen peroxide (H(2)O(2)) and nicotinamide adenine dinucleotide (NADH) with the linear range of 0.1 μM-39 mM and 5 × 10(-5) to 2.5 × 10(-4) M, respectively. It shows another linear range of 48.8-355.5 mM with the sensitivity of 12.3 μA mM(-1) cm(-2) for H(2)O(2). In particular, the PNR/FAD hybrid film has potential to replace some hemoproteins to be a cathode of biofuel cells and provide the biosensing system for glucose and ethanol.
authors: Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.
authors: Kuo Chiang
authors: KC
authors: Lin
authors: Yu Ching
authors: YC
authors: Lin
authors: Shen Ming
authors: SM
authors: Chen
mesh_terms: D000438:Alcohols / Q000032:analysis / Q000737:chemistry; D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000032:analysis* / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry; D009499:Neutral Red / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D000438:Alcohols; D004396:Coloring Agents; D011108:Polymers; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D009499:Neutral Red; D006861:Hydrogen Peroxide; D005947:Glucose