title: A high-throughput screen to identify novel calcineurin inhibitors.
abstract: Calcineurin is a eukaryotic protein phosphatase important for many signalling and developmental processes in cells. Inhibitors of this enzyme are used clinically and there is interest in identifying novel inhibitors for therapeutic applications. This report describes a high-throughput assay that can be used to screen natural or chemical libraries of compounds to identify new calcineurin inhibitors. The microtitre plate assay is based on a yeast reporter strain and was validated with known inhibitors and tested in a pilot screen of bacterial extracts.
authors: Marine Biotechnology Centre, Environmental Research Institute, Microbiology Department, University College Cork, Ireland.
authors: Lekha Menon
authors: LM
authors: Margassery
authors: Jonathan
authors: J
authors: Kennedy
authors: Fergal
authors: F
authors: O'Gara
authors: Alan D
authors: AD
authors: Dobson
authors: John P
authors: JP
authors: Morrissey
mesh_terms: D065095:Calcineurin Inhibitors; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017930:Genes, Reporter; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D065095:Calcineurin Inhibitors; D004791:Enzyme Inhibitors