title: TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system.
abstract: Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.
authors: TOYOTA Central R&D Labs, Inc, Nagakute, Aichi, Japan.
authors: Mamoru
authors: M
authors: Yamanishi
authors: Satoshi
authors: S
authors: Katahira
authors: Takashi
authors: T
authors: Matsuyama
mesh_terms: D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013728:Terminator Regions, Genetic; D019076:Transgenes
chemical_list: C014059:CYC1 protein, S cerevisiae; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); C465226:TDH3 protein, S cerevisiae; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase