title: Resistance performances of transgenic bt rice lines T(2A)-1 and T1c-19 against Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).
abstract: The transgenic Bacillus thuringiensis (Bt) rice, Oryza sativa L., lines T(2A)-1 and T1c-19 expressing Cry2A* and Cry1C* from 'Minhui 63' (MH63) were evaluated for resistance to newly hatched and third-instar larvae of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), by using detached leaf laboratory bioassays. Both T(2A)-1 and T1c-19 rice showed high C. medinalis resistance; however, the lethal time (LT)50 of larvae fed with T(2A)-1 rice was significantly longer than that of larvae fed with T1c-19 rice, implying T1c-19 rice was more toxic to C. medinalis larvae. Larval mortality after 4 d on nitrogen-free MH63 was 25.5% compared with 2.4% mortality on high nitrogen fertilizer (250 kg N/ha) plants. Larval mortality on high nitrogen T(2A)-1 plants declined by 20% compared with nitrogen-free plants. However, resistance in T1c-19 plants was unaffected by nitrogen fertilizer. C. medinalis moths preferred MH63 at both the seedling and grain milk stages for oviposition but not the T1c-19 and T(2A)-1 Bt rice lines.
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People's Republic of China.
authors: Xusong
authors: X
authors: Zheng
authors: Yajun
authors: Y
authors: Yang
authors: Hongxing
authors: H
authors: Xu
authors: Hao
authors: H
authors: Chen
authors: Baoju
authors: B
authors: Wang
authors: Yongjun
authors: Y
authors: Lin
authors: Zhongxian
authors: Z
authors: Lu
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D002681:China; D004731:Endotoxins / Q000633:toxicity*; D005260:Female; D005308:Fertilizers / Q000032:analysis; D006460:Hemolysin Proteins / Q000633:toxicity*; D007814:Larva; D009036:Moths / Q000187:drug effects*; D009584:Nitrogen / Q000032:analysis; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010572:Pest Control, Biological / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D005308:Fertilizers; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D009584:Nitrogen