title: Evolution of the protein corona of lipid gene vectors as a function of plasma concentration.
abstract: The concept that the effective unit of interest in the cell-nanomaterial interaction is the particle and its corona of associated proteins is emerging. Here we investigate the compositional evolution of the protein corona of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) and DOTAP/DNA lipoplexes over a wide range of plasma concentrations (2.5-80%). The composition of the hard corona of lipoplexes is quite stable, but that of CLs does evolve considerably. We show that the protein corona of CLs is made of both low-affinity and competitive-binding proteins whose relative abundance changes with the plasma concentration. This result may have deep biological implications for the application of lipid-based gene vectors both in vitro and in vivo.
authors: Department of Molecular Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy. giulio.caracciolo@uniroma1.it
authors: Giulio
authors: G
authors: Caracciolo
authors: Daniela
authors: D
authors: Pozzi
authors: Anna Laura
authors: AL
authors: Capriotti
authors: Chiara
authors: C
authors: Cavaliere
authors: Patrizia
authors: P
authors: Foglia
authors: Heinz
authors: H
authors: Amenitsch
authors: Aldo
authors: A
authors: Laganà
mesh_terms: D001667:Binding, Competitive; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D002412:Cations; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D010949:Plasma / Q000737:chemistry*; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D053838:Scattering, Small Angle; D014162:Transfection / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D001798:Blood Proteins; D002412:Cations; D008055:Lipids; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D004247:DNA