title: Bioinformatic survey of Haliotis midae microsatellites reveals a non-random distribution of repeat motifs.
abstract: Recent studies have shown the non-random distribution of microsatellite motifs between genomic regions within a particular species. This study investigates such microsatellite distributions in the genome of the economically important abalone Haliotis midae, via a bioinformatic survey. In particular, the association of specific repeat motifs to coding regions and transposable elements is investigated. An understanding of microsatellite genomic distribution will facilitate more efficient use and development of this popular molecular marker. A bias toward di- and tetranucleotide repeats was found in the H. midae genome. CA microsatellite units were the most abundant repeat motif, but were notably underrepresented in genic regions where GAGT repeats predominate. Approximately 17.5% and 21% of the microsatellites showed gene and/or transposable element associations, respectively. This could explain the high genomic frequencies of particular motifs across the genome and may allude to a possible functional role. The data presented in this study are the first to demonstrate such non-random dispersal of microsatellites in abalone and support previous findings arguing in favor of non-random distribution of repeat motifs.
authors: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland 7602, Republic of South Africa. roodt@sun.ac.za
authors: Clint
authors: C
authors: Rhode
authors: Rouvay
authors: R
authors: Roodt-Wilding
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D004251:DNA Transposable Elements; D049851:Gastropoda / Q000235:genetics*; D018895:Microsatellite Repeats; D016366:Open Reading Frames
chemical_list: D004251:DNA Transposable Elements