title: Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors.
abstract: Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a terminally differentiated somatic cell into a multipotent state without first establishing pluripotency. Here, we demonstrate that Sertoli cells derived from mesoderm can be directly converted into a multipotent state that possesses neural stem/progenitor cell properties. The induced neural stem/progenitor cells (iNSCs) express multiple NSC-specific markers, exhibit a global gene-expression profile similar to normal NSCs, and are capable of self-renewal and differentiating into glia and electrophysiologically functional neurons. iNSC-derived neurons stain positive for tyrosine hydroxylase (TH), γ-aminobutyric acid, and choline acetyltransferase. In addition, iNSCs can survive and generate synapses following transplantation into the dentate gyrus. Generation of iNSCs may have important implications for disease modeling and regenerative medicine.
authors: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors: Chao
authors: C
authors: Sheng
authors: Qinyuan
authors: Q
authors: Zheng
authors: Jianyu
authors: J
authors: Wu
authors: Zhen
authors: Z
authors: Xu
authors: Libin
authors: L
authors: Wang
authors: Wei
authors: W
authors: Li
authors: Haijiang
authors: H
authors: Zhang
authors: Xiao-Yang
authors: XY
authors: Zhao
authors: Lei
authors: L
authors: Liu
authors: Ziwei
authors: Z
authors: Wang
authors: Changlong
authors: C
authors: Guo
authors: Hua-Jun
authors: HJ
authors: Wu
authors: Zhonghua
authors: Z
authors: Liu
authors: Liu
authors: L
authors: Wang
authors: Shigang
authors: S
authors: He
authors: Xiu-Jie
authors: XJ
authors: Wang
authors: Zhiguo
authors: Z
authors: Chen
authors: Qi
authors: Q
authors: Zhou
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003001:Cloning, Molecular; D055724:Electrophysiological Phenomena; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism; D013569:Synapses / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; D002795:Choline O-Acetyltransferase