title: Biological and pathological studies of rosmarinic acid as an inhibitor of hemorrhagic Trimeresurus flavoviridis (habu) venom.
abstract: In our previous report, rosmarinic acid (RA) was revealed to be an antidote active compound in Argusia argentea (family: Boraginaceae). The plant is locally used in Okinawa in Japan as an antidote for poisoning from snake venom, Trimeresurus flavoviridis (habu). This article presents mechanistic evidence of RA's neutralization of the hemorrhagic effects of snake venom. Anti-hemorrhagic activity was assayed by using several kinds of snake venom. Inhibition against fibrinogen hydrolytic and collagen hydrolytic activities of T. flavoviridis venom were examined by SDS-PAGE. A histopathological study was done by microscopy after administration of venom in the presence or absence of RA. RA was found to markedly neutralize venom-induced hemorrhage, fibrinogenolysis, cytotoxicity and digestion of type IV collagen activity. Moreover, RA inhibited both hemorrhage and neutrophil infiltrations caused by T. flavoviridis venom in pathology sections. These results demonstrate that RA inhibited most of the hemorrhage effects of venom. These findings indicate that rosmarinic acid can be expected to provide therapeutic benefits in neutralization of snake venom accompanied by heat stability.
authors: Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya, 468-8503, Japan. hninthandaaung07@gmail.com
authors: Hnin Thanda
authors: HT
authors: Aung
authors: Toshiaki
authors: T
authors: Nikai
authors: Yumiko
authors: Y
authors: Komori
authors: Tsunemasa
authors: T
authors: Nonogaki
authors: Masatake
authors: M
authors: Niwa
authors: Yoshiaki
authors: Y
authors: Takaya
mesh_terms: D000818:Animals; D000997:Antivenins / Q000627:therapeutic use*; D028082:Boraginaceae; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000627:therapeutic use*; D024141:Collagen Type IV / Q000378:metabolism; D053630:Depsides / Q000627:therapeutic use*; D004355:Drug Stability; D004487:Edema / Q000188:drug therapy; D005340:Fibrinogen / Q000378:metabolism; D006470:Hemorrhage / Q000188:drug therapy* / Q000473:pathology; D006358:Hot Temperature; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D014757:Viper Venoms / Q000633:toxicity*; D017819:Viperidae; D000097123:Rosmarinic Acid
chemical_list: D000997:Antivenins; D002934:Cinnamates; D024141:Collagen Type IV; D053630:Depsides; D005340:Fibrinogen; D010936:Plant Extracts; D014757:Viper Venoms; D000097123:Rosmarinic Acid