title: Clinical and microbiologic evaluation, by real-time polymerase chain reaction, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole.
abstract: BACKGROUND
  The aim of the present study is to evaluate the clinical and microbiologic changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole in individuals with aggressive periodontitis.
  
  
  METHODS
  Fifteen individuals with aggressive periodontitis received non-surgical periodontal treatment and 45 days after completion of treatment were treated with antibiotics. Clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment, and 1 month after the use of antimicrobial agents. After 3 and 6 months, only clinical data were collected. The presence and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Treponema denticola (Td), and Dialister pneumosintes were determined by real-time polymerase chain reaction.
  
  
  RESULTS
  All clinical parameters, with the exception of clinical attachment level (CAL), had significantly (P <0.05) improved at the end of the third month after non-surgical therapy associated with antibiotics. There was significant (P <0.05) reduction in the quantities of Td and Tf. After 1 month, there were significant (P <0.05) reductions in the frequencies of Pg and Tf.
  
  
  CONCLUSIONS
  Non-surgical mechanical treatment associated with the use of amoxicillin and metronidazole led to an improvement in all clinical parameters studied, except for CAL, and significantly reduced the amount of subgingival Tf and Td.
authors: Department of Periodontology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, São Paulo, SP, Brazil.
authors: Ariana S
authors: AS
authors: Rodrigues
authors: Daniele Salami
authors: DS
authors: Lourenção
authors: Lidio G
authors: LG
authors: Lima Neto
authors: Claudio M
authors: CM
authors: Pannuti
authors: Rosario Dominguez Crespo
authors: RD
authors: Hirata
authors: Mario H
authors: MH
authors: Hirata
authors: Roberto F M
authors: RF
authors: Lotufo
authors: Giorgio
authors: G
authors: De Micheli
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D010520:Aggressive Periodontitis / Q000382:microbiology / Q000628:therapy*; D000658:Amoxicillin / Q000008:administration & dosage / Q000627:therapeutic use*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D058491:Bacterial Load / Q000187:drug effects; D001439:Bacteroides / Q000187:drug effects; D015342:DNA Probes; D003773:Dental Plaque / Q000382:microbiology; D003774:Dental Plaque Index; D004338:Drug Combinations; D005260:Female; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D005884:Gingival Hemorrhage / Q000382:microbiology / Q000628:therapy; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000187:drug effects; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000008:administration & dosage / Q000627:therapeutic use*; D017622:Periodontal Attachment Loss / Q000382:microbiology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000382:microbiology / Q000628:therapy; D016966:Porphyromonas gingivalis / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D048373:Treponema denticola / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D015342:DNA Probes; D004338:Drug Combinations; D005456:Fluorescent Dyes; D008795:Metronidazole; D000658:Amoxicillin