title: Inflammatory response and neutrophil functions in players after a futsal match.
abstract: Inflammatory response and neutrophil functions in players after a futsal match. J Strength Cond Res 26(9): 2507-2514, 2012-Futsal players suffer injuries resulting from muscle fatigue and contact or collision among players. Muscle lesions can be detected by measuring muscle lesion markers such as creatine kinase (CK) and lactate dehydrogenase (LDH) in plasma. After an initial lesion, there is an increase in the plasma levels of C-reactive protein (CRP) and proinflammatory cytokines. These mediators may activate neutrophils and contribute to tissue damage and increase susceptibility to invasive microorganisms. In this study, we investigated the effect of a futsal match on muscle lesion markers, cytokines, and CRP in elite players. The basal and stimulated neutrophil responsiveness after a match was also evaluated based on measurements of neutrophil necrosis, apoptosis, phagocytic capacity, reactive oxygen species (ROS) production, and cytokines (tumor necrosis factor-alpha [TNF-α], interleukin [IL]-8, IL-1β, IL-10, and IL-1ra) production. Blood samples were taken from 16 players (26.4 ± 3.2 years, 70.2 ± 6.9 kg, 59.7 ± 5.1 ml·kg·min, sports experience of 4.4 ± 0.9 years) before and immediately after a match. Exercise increased the serum activities of CK (2.5-fold) and LDH (1.3-fold). Playing futsal also increased the serum concentrations of IL-6 (1.6-fold) and CRP (1.6-fold). The TNF-α, IL-1β, IL-8, IL-1ra, and IL-10 serum levels were not modified in the conditions studied. The futsal match induced neutrophil apoptosis, as indicated by phosphatidylserine externalization (6.0-fold). The exercise induced priming of neutrophils by increasing ROS (1.3-fold), TNF-α (5.8-fold), and IL-1β (4.8-fold) released in nonstimulated cells. However, in the stimulated condition, the exercise decreased neutrophil function, diminishing the release of ROS by phorbol myristate acetate-stimulated neutrophils (1.5-fold), and the phagocytic capacity (1.6-fold). We concluded that playing futsal induces inflammation, primes and activates neutrophils, and reduces the efficiency of neutrophil phagocytosis immediately after a match.
authors: Post-Graduate Program in Human Movement Sciences, Institute of Physical Activity and Sport Sciences (ICAFE), Cruzeiro do Sul University, São Paulo, Brazil.
authors: Nivaldo R
authors: NR
authors: de Moura
authors: Maria F
authors: MF
authors: Cury-Boaventura
authors: Vinicius C
authors: VC
authors: Santos
authors: Adriana C
authors: AC
authors: Levada-Pires
authors: Joséricardo
authors: J
authors: Bortolon
authors: Jarlei
authors: J
authors: Fiamoncini
authors: Tania C
authors: TC
authors: Pithon-Curi
authors: Rui
authors: R
authors: Curi
authors: Elaine
authors: E
authors: Hatanaka
mesh_terms: D000328:Adult; D017209:Apoptosis; D002097:C-Reactive Protein / Q000032:analysis; D003402:Creatine Kinase / Q000097:blood; D016207:Cytokines / Q000097:blood; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000293:injuries / Q000502:physiology*; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism; D012916:Soccer / Q000293:injuries / Q000502:physiology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D002097:C-Reactive Protein; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase