title: Measurement of proton leak and electron leak in isolated mitochondria.
abstract: Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.
authors: Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA 94945-1400, USA.
authors: Charles
authors: C
authors: Affourtit
authors: Casey L
authors: CL
authors: Quinlan
authors: Martin D
authors: MD
authors: Brand
mesh_terms: D000818:Animals; D004583:Electrons; D007700:Kinetics; D053078:Membrane Potential, Mitochondrial; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D011522:Protons; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011522:Protons