title: Functional analysis of a putative Dothistromin toxin MFS transporter gene.
abstract: Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, dotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing dotC, or with a dotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of dotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.
authors: Bio-Protection Research Centre, Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand. r.e.bradshaw@massey.ac.nz
authors: Rosie E
authors: RE
authors: Bradshaw
authors: Zhilun
authors: Z
authors: Feng
authors: Arne
authors: A
authors: Schwelm
authors: Yongzhi
authors: Y
authors: Yang
authors: Shuguang
authors: S
authors: Zhang
mesh_terms: D001203:Ascomycota / Q000235:genetics; D028223:Pinus