title: Tyrosinase-modified carbon felt-based flow-biosensors: the role of ultra-sonication in shortening the enzyme immobilization time and improving the sensitivity for p-chlorophenol.
abstract: Tyrosinase (TYR) was covalently immobilized onto amino-functionalized carbon felt surface via glutaraldehyde-coupling under ultrasonic treatment for 10 min. The resulting TYR-immobilized carbon felt was used as a working electrode unit of bioelectrocatalytic flow-through detector for TYR substrates (catechol, p-chlorophenol (p-CP), p-cresol, phenol etc.). Cathodic peak currents based on the electroreduction of enzymatically produced o-quinones were detected at -50 mV vs. Ag/AgCl. Compared with previous work in which TYR was immobilized onto amino-functionalized carbon felt for 16 hr without the ultrasonic treatment, we succeeded in (1) shortening the enzyme immobilization time from 16 hr to 10 min, (2) enhancing the sensitivity of p-CP, and (3) improving the operational stability of p-CP. The ultrasonic treatment during the TYR immobilization step would lead to certain changes in the structure of the immobilized TYR and the morphology of the immobilized TYR-layer on the carbon felt surface.
authors: Department of Material Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan. q8003ctz@sit.ac.jp
authors: Yue
authors: Y
authors: Wang
authors: Yasushi
authors: Y
authors: Hasebe
mesh_terms: D000891:Anti-Infective Agents, Local / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism*; D002733:Chlorophenols / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism*; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013010:Sonication
chemical_list: D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004800:Enzymes, Immobilized; C029107:4-chlorophenol; D002244:Carbon; D014442:Monophenol Monooxygenase