title: Brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing the NF-κB pathway in human dermal fibroblasts.
abstract: Brazilin (7, 11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6H)-tetrol), the major component of Caesalpinia sappan L., is a natural red pigment used for histological staining. Recent studies have shown that brazilin exhibits distinct biological effects, including anti-hepatotoxicity, antiplatelet activity, and anti-inflammatory activities. In the present study, we evaluated the effects of brazilin on MMP-1 and -3 expressions in human dermal fibroblasts exposed to ultraviolet B (UVB) irradiation. Brazilin showed protective effect on UVB-induced loss of cell viability of fibroblasts. Brazilin also blocked significantly UVB-induced Reactive Oxygen Species generation in fibroblasts. Brazilin inhibited UVB-induced MMP-1/3 expressions and secretions in a dose-dependent manner. Moreover, UVB-induced NF-κB activation was completely blocked by treatment with brazilin. These findings suggest that brazilin inhibits UVB-induced MMP-1/3 expressions and secretions by suppressing of NF-κB activation in human dermal fibroblasts. Thus, brazilin might be used as a potential agent for treatment of UV-induced skin photoaging.
authors: Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 560-182, Republic of Korea.
authors: Young-Rae
authors: YR
authors: Lee
authors: Eun-Mi
authors: EM
authors: Noh
authors: Ji-Hye
authors: JH
authors: Han
authors: Jeong-Mi
authors: JM
authors: Kim
authors: Jin-Ki
authors: JK
authors: Hwang
authors: Bo-Mi
authors: BM
authors: Hwang
authors: Eun-Yong
authors: EY
authors: Chung
authors: Byeong-Soo
authors: BS
authors: Kim
authors: Sung-Ho
authors: SH
authors: Lee
authors: Seung Jin
authors: SJ
authors: Lee
authors: Jong-Suk
authors: JS
authors: Kim
mesh_terms: D001578:Benzopyrans / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D053505:Matrix Metalloproteinases, Secreted / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001578:Benzopyrans; D016328:NF-kappa B; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D004247:DNA; D053505:Matrix Metalloproteinases, Secreted; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1; C044362:brazilin