title: Effects of high pressure treatment of rabbit Longissimus dorsi muscles on the microsomal membranes.
abstract: High pressure treatment (150 MPa for 10 min at 35°C) of rabbit longissimus dorsi muscles reduced the yield of a heavy microsomal preparation (8000-28 000 g) by approximately 90%. This was shown to be due to the loss of the sarcoplasmic reticulum, the major component of microsomal preparations, from untreated muscles. While microsomal preparations from pressure-treated muscles were shown to have lost all of their (Ca(2+), Mg(2+))-ATPase activity they retained all their Mg(2+)-ATPase (basal) activity. Sucrose density gradient centrifugation of these preparations yielded two fractions. By reason of its low density, high cholesterol content, high Mg(2+)-ATPase activity, SDS-gel protein profile and solubility in lysolecithin, the lightest fraction appeared to be derived from the transvers tubules. The heavier fraction, present in greater quantity but with a lower Mg(2+)-ATPase activity than the light fraction, was probably derived from the sarcolemma.
authors: CSIRO, Division of Food Research, Meat Research Laboratory, Cannon Hill, Queensland 4170, Australia.
authors: D J
authors: DJ
authors: Horgan
authors: R
authors: R
authors: Kuypers