title: Mechanism of regulation of PPARG expression of mesenchymal stem cells by osteogenesis-mimicking extracellular matrices.
abstract: The mechanism of regulation of PPARG expression during the osteogenesis of human mesenchymal stem cells (MSCs) was investigated on an extracellular matrix (ECM) model that mimicked the stepwise osteogenesis ECM. Three matrices that mimicked the ECM of MSCs (stem cell matrices), the early stage ECM (early stage matrices), and the late stage ECM (late stage matrices) of osteogenesis were prepared and compared. The matrices showed different effects on the Wnt/β-catenin signal. The β-catenin signal was activated by endogenous Wnt through interaction with chondroitin sulfate chains to suppress PPARG expression on the stem cell matrices and early stage matrices but not on late stage matrices.
authors: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
authors: Takashi
authors: T
authors: Hoshiba
authors: Naoki
authors: N
authors: Kawazoe
authors: Guoping
authors: G
authors: Chen
mesh_terms: D002454:Cell Differentiation; D002809:Chondroitin Sulfates / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D060509:Wnt3A Protein / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D047495:PPAR gamma; D060509:Wnt3A Protein; D051176:beta Catenin; D002809:Chondroitin Sulfates