title: Reduction of oxidative damage reflects a better kidney transplantation outcome.
abstract: OBJECTIVE
  DNA fragmentation is one of the typical features of apoptosis, frequently induced by oxidative stress. Increased oxidative stress is known to be related to several pathological processes. In this study, we assessed oxidative damage in the early follow-up period after kidney transplantation measuring DNA oxidation and fragmentation of mononuclear cells and the circulating levels of inflammatory cytokines.
  
  
  METHODS
  Blood samples from 30 kidney transplant recipients were collected before transplantation and after 2 days, 1 month and 6 months. Oxidative DNA fragmentation was measured by Comet Assay, whereas DNA oxidation was evaluated measuring 8-OHdG leukocyte levels. Serum IL-1β, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α were assayed using a multiplex ELISA analysis.
  
  
  RESULTS
  At 6 months after transplantation, a significant reduction in DNA fragmentation and IL-6 plasma levels was observed; DNA oxidation was higher in patients with a worse outcome, with delayed graft function and low nutritional status. We also found a correlation of IL-6 and IL-10 levels with DNA fragmentation and of IL-10 levels with DNA oxidation.
  
  
  CONCLUSIONS
  Low levels of oxidation and apoptosis at 6 months after transplantation correlate with a better recovery of renal function in kidney allografts. The measurement of cytokine levels confirmed a reduction of inflammatory parameters within 6 months of follow-up.
authors: Section of Nephrology, Department of Internal Medicine, Aging and Renal Diseases, University of Bologna, Italy. gaetano.lamanna@unibo.it
authors: Gaetano
authors: G
authors: La Manna
authors: Nicole
authors: N
authors: Lanci
authors: Elena
authors: E
authors: Della Bella
authors: Giorgia
authors: G
authors: Comai
authors: Maria Laura
authors: ML
authors: Cappuccilli
authors: Katia
authors: K
authors: Nisi
authors: Paola
authors: P
authors: Todeschini
authors: Elisa
authors: E
authors: Carretta
authors: Maria Piera
authors: MP
authors: Scolari
authors: Sergio
authors: S
authors: Stefoni
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D020552:Comet Assay; D004247:DNA / Q000737:chemistry; D053938:DNA Fragmentation; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006085:Graft Survival; D006801:Humans; D007249:Inflammation; D016753:Interleukin-10 / Q000737:chemistry; D016030:Kidney Transplantation / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D016753:Interleukin-10; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D010100:Oxygen