title: Protective effect of hypoxic preconditioning on hypoxic-ischemic injured newborn rats.
abstract: Brief episodes of cerebral hypoxia-ischemia cause transient ischemic tolerance to subsequent ischemic events that are otherwise lethal. This study was conducted to evaluate the protective effect of hypoxic preconditioning on hypoxic-ischemic injury in the neonatal rat and the persistence of a protective window after hypoxic preconditioning. The rats were preconditioned with hypoxia (8% oxygen, 92% nitrogen) for three hours, subjected to ischemia using ligation of the right common carotid artery, and then exposed to another three hours of hypoxia. Using proton magnetic resonance spectroscopy, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) staining, and morphologic scores, this study shows that hypoxic preconditioning 6-hr to 1-day before hypoxic-ischemic injury increases survival rates and has neuroprotective effects against subsequent hypoxic-ischemic injury. The mechanism of the protective effects of hypoxic preconditioning in the newborn rat brain may involve downregulation of apoptotic cell death.
authors: Department of Pediatrics, Hanyang University College of Medicine, Seoul, Korea.
authors: Hyun-Kyung
authors: HK
authors: Park
authors: In-Joon
authors: IJ
authors: Seol
authors: Ki-Soo
authors: KS
authors: Kim
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000032:analysis; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002339:Carotid Arteries / Q000601:surgery; D003401:Creatine / Q000032:analysis; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D020287:In Situ Nick-End Labeling; D019194:Ischemic Preconditioning / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate
chemical_list: D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine