title: Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro.
abstract: OBJECTIVE
  Restoration of epithelial continuity in the intestinal surface after extensive destruction is important since intestinal epithelial cells stand as a boundary between the body's internal and external environment. Polysaccharides from Ganoderma lucidum (Gl-PS) may benefit intestinal epithelial wound healing in different aspects, which awaits clarification. To identify potential effects, a non-transformed small-intestinal epithelial cell line, IEC-6 cells, was used.
  
  
  METHODS
  Effects on epithelial cell proliferation, migration, morphology of differentiation and transforming growth factor beta (TGF-β) protein expression, as well as the cellular ornithine decarboxylase (ODC) mRNA and c-Myc mRNA expression, were assessed, respectively, by MTT assay, wound model in vitro, observation under a microscope after hematoxylin and eosin staining, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction assays.
  
  
  RESULTS
  It was shown that Gl-PS stimulated IEC-6 cell proliferation and migration significantly in a dose-dependent manner; 10 µg/ml Gl-PS improved the morphology of differentiation in IEC-6 cells. Inefficacy in expression of TGF-β in IEC-6 cells indicated a possible TGF-β independent action of Gl-PS. However, Gl-PS increased ODC mRNA and c-Myc mRNA expression in a dose-dependent manner, indicating, at least partially possible involvement of ODC and c-Myc gene expression in improvement of intestinal wound healing.
  
  
  CONCLUSIONS
  These results suggest the potential usefulness of Gl-PS to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
authors: Department of Pharmacology, Basic Medical School, Peking University Health Science Center, Beijing Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, China.
authors: Li-Xin
authors: LX
authors: Sun
authors: Li-Hua
authors: LH
authors: Chen
authors: Zhi-Bin
authors: ZB
authors: Lin
authors: Ying
authors: Y
authors: Qin
authors: Jia-Qi
authors: JQ
authors: Zhang
authors: Jing
authors: J
authors: Yang
authors: Jie
authors: J
authors: Ma
authors: Ting
authors: T
authors: Ye
authors: Wei-Dong
authors: WD
authors: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004847:Epithelial Cells / Q000187:drug effects; D007413:Intestinal Mucosa / Q000473:pathology*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020940:Reishi / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011134:Polysaccharides; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D009954:Ornithine Carbamoyltransferase