title: Distribution of spontaneous gyrA mutations in 97 fluoroquinolone-resistant Helicobacter pylori isolates collected in France.
abstract: We determined the prevalence of gyrA mutations conferring fluoroquinolone resistance in 97 Helicobacter pylori isolates collected in France from 2007 to 2010. Ninety-four harbored one or two mutations already found in the quinolone resistance determining region (QRDR) of gyrA (for T87I, n = 23; for N87K, n = 32; for D91N, n = 30; for D91G, n = 7; for D91Y, n = 6), 2 harbored a mutation never previously described (D91H and A88P), and one strain was resistant (ciprofloxacin MIC of 8 mg/liter) without a detected mutation conferring this resistance in gyrA or gyrB genes.
authors: EA 4331 LITEC, Université de Poitiers, CHU de Poitiers, Laboratoire de Bactériologie-Hygiène, Poitiers, France.
authors: Magali
authors: M
authors: Garcia
authors: Josette
authors: J
authors: Raymond
authors: Martine
authors: M
authors: Garnier
authors: Julie
authors: J
authors: Cremniter
authors: Christophe
authors: C
authors: Burucoa
mesh_terms: D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000627:therapeutic use*; D027081:DNA Gyrase / Q000235:genetics*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000627:therapeutic use; D005602:France; D016481:Helicobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D024841:Fluoroquinolones; D002939:Ciprofloxacin; D027081:DNA Gyrase