title: Neoglycolipid-based oligosaccharide microarray system: preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses.
abstract: Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein-carbohydrate interactions both in endogenous recognition systems and pathogen-host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques - from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins.
authors: The Glycosciences Laboratory, Department of Medicine, Imperial College London, Hammersmith Campus, London, UK. yan.liu2@imperial.ac.uk
authors: Yan
authors: Y
authors: Liu
authors: Robert A
authors: RA
authors: Childs
authors: Angelina S
authors: AS
authors: Palma
authors: Maria A
authors: MA
authors: Campanero-Rhodes
authors: Mark S
authors: MS
authors: Stoll
authors: Wengang
authors: W
authors: Chai
authors: Ten
authors: T
authors: Feizi
mesh_terms: D003101:Collodion / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D009844:Oligosaccharides; D003101:Collodion