title: Automated adherent human cell culture (mesenchymal stem cells).
abstract: Human cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT.
authors: Centre for Biological Engineering, Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Leicestershire, UK. R.J.Thomas@lboro.ac.uk
authors: Robert
authors: R
authors: Thomas
authors: Elizabeth
authors: E
authors: Ratcliffe
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation* / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism