title: Sox9 is upstream of microRNA-140 in cartilage.
abstract: MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.
authors: Department of Orthopaedic Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors: Yukio
authors: Y
authors: Nakamura
authors: Xinjun
authors: X
authors: He
authors: Hiroyuki
authors: H
authors: Kato
authors: Shigeyuki
authors: S
authors: Wakitani
authors: Tatsuya
authors: T
authors: Kobayashi
authors: Sumiko
authors: S
authors: Watanabe
authors: Atsumi
authors: A
authors: Iida
authors: Hideaki
authors: H
authors: Tahara
authors: Matthew L
authors: ML
authors: Warman
authors: Ramida
authors: R
authors: Watanapokasin
authors: John H
authors: JH
authors: Postlethwait
mesh_terms: D000818:Animals; D001665:Binding Sites; D002356:Cartilage / Q000254:growth & development* / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D055758:SOX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development*
chemical_list: D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D034741:RNA, Small Interfering; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C465283:WWP2 protein, human; D044767:Ubiquitin-Protein Ligases