title: Tryptose phosphate broth improves Rickettsia felis replication  in mammalian cells.
abstract: In cell culture, Rickettsia felis grows only at low temperatures (< 31 °C). Therefore, its ability to enter, survive and grow in cell lines has primarily been tested in cells derived from amphibians and arthropods, which naturally grow at low temperatures, and only infrequently in mammalian cells. We subcultured R. felis in mammalian cells for more than 10 passages using media supplemented with tryptose phosphate broth (TPB) and found that TPB is critical for optimal growth of R. felis in mammalian cells.
authors: URMITE UMR CNRS 6236-IRD198, Faculté de Médecine et de Pharmacie, Université de la Méditerranée, Marseille, France.
authors: Watcharee
authors: W
authors: Saisongkorh
authors: Khalid
authors: K
authors: El Karkouri
authors: Jean-Yves
authors: JY
authors: Patrice
authors: Annick
authors: A
authors: Bernard
authors: Jean-Marc
authors: JM
authors: Rolain
authors: Didier
authors: D
authors: Raoult
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry*; D008322:Mammals; D041161:Rickettsia felis / Q000254:growth & development*; D013696:Temperature
chemical_list: D003470:Culture Media