title: Inhalative IL-10 attenuates pulmonary inflammation following hemorrhagic shock without major alterations of the systemic inflammatory response.
abstract: Several studies report immunomodulatory effects of endogenous IL-10 after trauma. The present study investigates the effect of inhalative IL-10 administration on systemic and pulmonary inflammation in hemorrhagic shock. Male C57/BL6 mice (8 animals per group) were subjected to pressure-controlled hemorrhagic shock for 1.5 hrs followed by resuscitation and inhalative administration of either 50 μL PBS (Shock group) or 50 μg/kg recombinant mouse IL-10 dissolved in 50 μL PBS (Shock + IL-10 group). Animals were sacrificed after 4.5 hrs of recovery and serum IL-6, IL-10, KC, and MCP-1 concentrations were measured with ELISA kits. Acute pulmonary inflammation was assessed by pulmonary myeloperoxidase (MPO) activity and pulmonary H&E histopathology. Inhalative IL-10 administration decreased pulmonary inflammation without altering the systemic concentrations of IL-6, IL-10, and KC. Serum MCP-1 levels were significantly reduced following inhalative IL-10 administration. These findings suggest that inhalative IL-10 administration may modulate the pulmonary microenvironment without major alterations of the systemic inflammatory response, thus minimizing the potential susceptibility to infection and sepsis.
authors: Department of Orthopaedic Trauma Surgery, Faculty of Medicine, RWTH Aachen University, Pauwelsstraβe 30, 52074 Aachen, Germany. pkobbe@ukaachen.de
authors: Philipp
authors: P
authors: Kobbe
authors: Philipp
authors: P
authors: Lichte
authors: Helen
authors: H
authors: Schreiber
authors: Lucy Kathleen
authors: LK
authors: Reiss
authors: Stefan
authors: S
authors: Uhlig
authors: Hans-Christoph
authors: HC
authors: Pape
authors: Roman
authors: R
authors: Pfeifer
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D016207:Cytokines / Q000097:blood; D016753:Interleukin-10 / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology / Q000627:therapeutic use; D015850:Interleukin-6 / Q000097:blood; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011014:Pneumonia / Q000097:blood / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control*; D012771:Shock, Hemorrhagic / Q000097:blood / Q000150:complications* / Q000276:immunology
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016753:Interleukin-10