title: Overexpression of survivin is correlated with increased invasion and metastasis of colorectal cancer.
abstract: BACKGROUND
  The aim of this study was to investigate the association of survivin expression with metastasis of colorectal cancer (CRC).
  
  
  METHODS
  RT-PCR and Western blot assays were performed to detect survivin expression in CRC cells and normal intestinal epithelial cell. The expression of survivin gene was also detected in 15 CRC tissues, surrounding and adjacent colon tissues. Moreover, survivin expression in 48 CRC tissues with or without lymph node metastasis was analyzed. Multivariate analysis for lymph node metastasis was performed using logistic regression model. RNA interference was used to inhibit survivin expression in CRC cells and analyze its effect on invasion and metastasis of CRC cells.
  
  
  RESULTS
  The expression levels of survivin mRNA and protein were higher in CRC cells than in normal intestinal epithelial cell line. The average levels of survivin mRNA and protein were higher in CRC tissues than surrounding or adjacent colon tissues (P < 0.05). High survivin expression was an independent factor for predicting lymph node metastasis of CRC (P = 0.043). RNAi-mediated survivin knockdown could significantly inhibit in vitro invasion and in vivo metastasis of CRC cells, which might be inactivation of matrix metalloproteinases.
  
  
  CONCLUSIONS
  Targeting survivin will be a potential strategy to suppress metastasis of CRC.
authors: Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu Province, China. xiaoyuanchu55@yahoo.com.cn
authors: Xiao-Yuan
authors: XY
authors: Chu
authors: Long-Bang
authors: LB
authors: Chen
authors: Jing-Hua
authors: JH
authors: Wang
authors: Quan-Sheng
authors: QS
authors: Su
authors: Ji-Rong
authors: JR
authors: Yang
authors: Yong
authors: Y
authors: Lin
authors: Li-Jun
authors: LJ
authors: Xue
authors: Xiao-Bei
authors: XB
authors: Liu
authors: Xiao-Bei
authors: XB
authors: Mo
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin