title: New and old NMR experiments for the resonance assignment of complex oligosaccharides--application to a cyclodextrin derivative.
abstract: The complete assignment of the (1)H and (13)C sugar resonances in mono-3,6-anhydro-heptakis(2,3-O-methyl)-hexakis(6-O-methyl)-β-cyclodextrin, an asymmetrically functionalized β-cyclodextrin, was carried out by means of 2D NMR experiments. The TOCSY and the homonuclear multiple relay COSY spectra provided most of the (1)H assignments. The multiplicity edited HSQC and a set of F(1) selective HSQC-TOCSY and multiple relay HSQC-COSY spectra gave access to most of the (13)C chemical shifts. The latter were fully and accurately determined by means of a pair of complementary, highly folded HSQC-TOCSY spectra. The TOCSY-ROESY and ROESY-TOCSY spectra yielded the sequential assignment of the sugar units. A high resolution F(1) selective F(1) decoupled version of the TOCSY-ROESY experiment was recorded.
authors: Institut de Chimie Moléculaire de Reims, Université de Reims Champagne-Ardenne, CNRS UMR 6229, Reims, France.
authors: B
authors: B
authors: Plainchont
authors: A
authors: A
authors: Martinez
authors: S
authors: S
authors: Tisse
authors: J-P
authors: JP
authors: Bouillon
authors: F
authors: F
authors: Pilard
authors: J-M
authors: JM
authors: Wieruszeski
authors: G
authors: G
authors: Lippens
authors: D
authors: D
authors: Jeannerat
authors: J-M
authors: JM
authors: Nuzillard