title: Oral rehydration salt-liquid as an alternative storage medium--a preliminary study.
abstract: OBJECTIVE
  To assess the efficacy of Oral Rehydration Salt-Liquid (ORS-L) in the maintenance of the viability of Periodontal ligament cells (PDL).
  
  
  METHODS
  Twenty freshly extracted teeth were used for this study. They were then randomly divided into 3 groups: Positive control group--5 teeth which were immediately subjected to collagenase assay, without immersing in ORS-L; Negative control group--5 teeth with an extra oral dry time of 24 hours, followed by subjecting to collagense assay without immersing in ORS-L and Test group (ORS-L)--10 teeth with an extra oral dry period of 30 minutes, followed by immersion in ORS-L for a period of 45 minutes and then subjected to collagenase assay. The mean number of viable PDL cells were counted on a hemocytometer under 20X magnification.
  
  
  RESULTS
  The mean number of viable PDL cells was highest in the positive control group. In comparison to the negative control group, the test group showed a higher number of viable PDL cells.
  
  
  CONCLUSIONS
  The study found that ORS-L was an effective solution in maintaining the viability of PDL cells.
authors: Department of Pedodontics And Preventive Dentistry, The Oxford Dental College, Hospital And Research Centre, Bommanahalli, Hosur Road, Bangalore-560068, Karnataka, India. drpriyapedo@yahoo.com
authors: P
authors: P
authors: Subramaniam
authors: U
authors: U
authors: Eswara
authors: K L
authors: KL
authors: Girish Babu
authors: B
authors: B
authors: Vardhan
mesh_terms: D001639:Bicarbonates; D002452:Cell Count; D002470:Cell Survival; D017364:Collagenases / Q000378:metabolism; D003890:Desiccation; D005947:Glucose; D006801:Humans; D019188:Organ Preservation Solutions; D010513:Periodontal Ligament / Q000166:cytology*; D011189:Potassium Chloride; D015532:Rehydration Solutions; D012965:Sodium Chloride; D014084:Tooth Avulsion / Q000628:therapy
chemical_list: D001639:Bicarbonates; D019188:Organ Preservation Solutions; D015532:Rehydration Solutions; C044142:World Health Organization oral rehydration solution; D012965:Sodium Chloride; D011189:Potassium Chloride; D017364:Collagenases; D005947:Glucose