title: [Construction of a recombination plasmid labelled with green fluorescence and its application in Clonostachys rosea].
abstract: OBJECTIVE
  We constructed a recombinant eukaryotic expression vector harboring green fluorescent protein (GFP) and the hygromycin resistance gene hph, and observed its expression in Clonostachys rosea.
  
  
  METHODS
  We used PCR, enzyme digestion, phosphorylation, ligation and transformation to construct the plasmid. Using protoplast preparation and transformation technologies, we expressed the plasmid in the fungi C. rosea.
  
  
  RESULTS
  We created the eukaryotic expression vector, transformed it into C. rosea and observed green fluorescence with fluorescence microscope.
  
  
  CONCLUSIONS
  The successful construction of the pANGH3 recombinant plasmid and its expression in C. rosea establishes a new model for studying fungal infection mechanisms.
authors: School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China. qiuhongniu@yahoo.com.cn
authors: Qiuhong
authors: Q
authors: Niu
authors: Tao
authors: T
authors: Ke
authors: Lin
authors: L
authors: Zhang
authors: Fengli
authors: F
authors: Hui
authors: Jibao
authors: J
authors: Chen
mesh_terms: D002934:Cinnamates / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D006999:Hypocreales / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014170:Transformation, Genetic
chemical_list: D002934:Cinnamates; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D006921:Hygromycin B; C026273:hygromycin A