title: Determination of the new anthelmintic monepantel and its sulfone metabolite in milk and muscle using a UHPLC-MS/MS and QuEChERS method.
abstract: This is the first paper to report a method for the detection of the new anthelmintic monepantel and its sulfone metabolite in goat's milk and ovine muscle. Samples were extracted and purified using a modified QuEChERS method. A concentration step was included when analyzing in the low μg kg(-1) range. Analysis was carried out by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in a 13min run time using atmospheric pressure electrospray ionisation in the negative mode (ESI(-)) and multiple reaction monitoring (MRM) scanning. Monepantel (m/z 472) and monepantel-sulfone (m/z 504) both had product ions at m/z 186 and m/z 166. The method has been single-laboratory validated according to the 2002/657/EC guidelines. The mean recovery in milk was 108 and 106% for monepantel and monepantel-sulfone, respectively. The mean recovery in muscle was 109 and 108% for monepantel and monepantel-sulfone, respectively. The coefficients of variation for the within laboratory repeatability and reproducibility were ≤6.4% in milk and ≤14.2% in muscle. The decision limits (CCα) in milk were 2.20 and 2.08 μg kg(-1) for monepantel and monepantel-sulfone, respectively. The decision limits (CCα) in muscle were 771 and 746 μg kg(-1) for monepantel and monepantel-sulfone, respectively.
authors: Teagasc Food Research Centre, Ashtown, Dublin, Ireland. brian.kinsella@mycit.ie
authors: Brian
authors: B
authors: Kinsella
authors: Patrick
authors: P
authors: Byrne
authors: Helen
authors: H
authors: Cantwell
authors: Martin
authors: M
authors: McCormack
authors: Ambrose
authors: A
authors: Furey
authors: Martin
authors: M
authors: Danaher
mesh_terms: D000607:Aminoacetonitrile / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D006041:Goats; D057230:Limit of Detection; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012756:Sheep; D013450:Sulfones / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D013450:Sulfones; C553923:monepantel sulfone; D000607:Aminoacetonitrile; C533978:monepantel