title: Allogeneic donor peripheral blood "stem cell" apheresis: prospective comparison of two apheresis systems.
abstract: BACKGROUND
  Granulocyte-colony-stimulating factor-mobilized peripheral blood stem cells, collected by white blood cell apheresis, are used for more than 80% of allogeneic and most autologous hematopoietic stem cell transplantations. Optimal donor and recipient outcomes require maximized stem cell collection efficiency and minimized non-target cell contamination. Therefore, improved apheresis technology is desirable. The safety and feasibility of apheresis collections with the novel, electronics-assisted apheresis system Spectra Optia v.5.0 (CaridianBCT) were recently demonstrated. An unpublished optimization trial had furthermore determined that different settings than manufacturer-installed default might result in improved apheresis yields.
  
  
  METHODS
  The first prospective comparison of allogeneic peripheral blood stem cell apheresis with the Spectra Optia versus the COBE Spectra (CaridianBCT) mononuclear cell (MNC) in a routine clinical setting is reported here; "optimized" machine settings were used. Assessed variables included collection efficiency, product characteristics, donor outcomes, and frequency of operator interventions. Outcomes were additionally compared with historical data from the Spectra Optia in default mode.
  
  
  RESULTS
  The mean CD34+ cell collection efficiency CE1 was 7.9% greater with the Spectra Optia than with the COBE Spectra MNC. Variability of outcomes was equally great. Reduced platelet (PLT) attrition necessitated 90% fewer autologous PLT reinfusions. Spectra Optia products contained 50% fewer red blood cells, but 50% more granulocytic lineage cells. Less operator input was required, although 26% of Spectra Optia apheresis procedures required triggering of the first chamber flush. Apheresis yield and collection efficiency were also markedly greater than in default-mode Spectra Optia collections.
  
  
  CONCLUSIONS
  Using optimized machine settings, peripheral blood stem cell apheresis outcomes with the automated apheresis system Spectra Optia exceed results with the COBE Spectra MNC or the Spectra Optia in the default mode.
authors: German Red Cross Blood Service Baden-Wuerttemberg-Hesse and the Institute for Transfusion Medicine and Immunohematology, Goethe-University, Frankfurt, Germany.
authors: Susanne
authors: S
authors: Brauninger
authors: Heike
authors: H
authors: Bialleck
authors: Kristin
authors: K
authors: Thorausch
authors: Tom
authors: T
authors: Felt
authors: Erhard
authors: E
authors: Seifried
authors: Halvard
authors: H
authors: Bonig
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods*; D001782:Blood Donors; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008297:Male
chemical_list: D018952:Antigens, CD34