title: [The acceleration of titanium implant osseointegration by low intensity pulsed ultrasound: an experimental study in rats].
abstract: OBJECTIVE
  To establish an animal model of titanium implant and to investigate the effects of low intensity pulsed ultrasound (LIPUS) on the implant osseointegration.
  
  
  METHODS
  Twenty-four female Sprague Dawley (SD) rats were used. Titanium implants were inserted into tibia metaphysis bilaterally in each SD rat. Since the second day following implant surgery, LIPUS stimulation were carried out around the implant on the right side for 20 minutes per day, and implants on the left side remain untouched as self-control. Eight rats were sacrificed and tibiae specimens were harvested on the end of the 4th, 8th, and 12th week respectively. X-ray picture, micro-CT analysis and histological sections were performed to evaluate the effects of LIPUS on the implant osseointegration.
  
  
  RESULTS
  Osseointegration at the interface between the implant and bone tissue was observed bilaterally. In the micro-CT histomorphometry analysis, the percentage of osseointegration (%OI) of experiment side were (45.708 ± 3.316)%, (46.231 ± 1.954)%, and (46.807 ± 1.451)% on the end of the 4th, 8th, and 12th week. Meanwhile, the %OI of control side were (43.021 ± 3.558)%, (44.272 ± 3.023)%, and (44.894 ± 4.215)% respectively. The %OI on the 4th weekend, bone volume ratio (BV/TV) on the 4th and 8th weekend, trabecular thickness (Tb. Th) and degree of anisotropy (DA) on the 8th and 12th weekend of the experiment side tibia were significantly greater than those on the control side (P < 0.05). The histological examination showed that the thickness of new bone around the implant and the bone-implant contact area was obviously greater in the LIPUS treated side compared to the control side.
  
  
  CONCLUSIONS
  The LIPUS therapy may accelerate the bone healing and osseointegration at the interface between titanium implant and bone, and promote remodeling of bone trabecula on the early stage.
authors: Department of Prosthodontics, Sichuan University, Chengdu, China.
authors: Hong-bo
authors: HB
authors: Zhou
authors: Yong-fu
authors: YF
authors: Hou
authors: Wen-chuan
authors: WC
authors: Chen
authors: Jie-fei
authors: JF
authors: Shen
authors: Jian
authors: J
authors: Wang
authors: Zhi-min
authors: ZM
authors: Zhu
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D005260:Female; D020007:Implants, Experimental; D016348:Osseointegration; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000601:surgery*; D014025:Titanium / Q000737:chemistry*; D014464:Ultrasonic Therapy / Q000379:methods*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D014025:Titanium