title: Neurite outgrowth of PC12 cells on diX (parylene) family materials.
abstract: We investigated neuronal cell differentiation, particularly neurite outgrowth, on the surface of diX H and diX AM using an in vitro examination of a neuron-like rat pheochromocytoma cell line, PC12. diX H and diX AM are in the parylene family of diX C (or Parylene-C), which is widely used as a novel coating material to insulate neural electrodes, and they have been recently commercialized; diX H and diX AM offer different features of biocompatibility. Previously, we found that these new parylene materials have high cell adhesiveness to neuronal cells whereas the adhesiveness of diX C is extremely low. However, their cell differentiation remains unknown although neuronal cell differentiation plays a crucial role in their development and regeneration. This study showed that almost all PC12 cells adhering to the surface of diX AM and diX H were differentiated, but the neurite outgrowth was significantly larger on diX H than that on diX AM and a conventional polystyrene culture dish. The result suggests that diX H may be advantageous as a biocompatible coating material for a scaffold, which can be used on virtually any substrate to get various configurations in neural devices.
authors: Dept. of Bio-Application and System Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.
authors: Takayuki
authors: T
authors: Hoshino
authors: Itsuro
authors: I
authors: Saito
authors: Haruka
authors: H
authors: Takano
authors: Kazuyuki
authors: K
authors: Samejima
authors: Kunihiko
authors: K
authors: Mabuchi
authors: Yasuhiro X
authors: YX
authors: Kato
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D016716:PC12 Cells; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D014992:Xylenes / Q000494:pharmacology*
chemical_list: D011108:Polymers; D014992:Xylenes; C011055:parylene