title: Difference in intraosseous blood vessel volume and number in osteoporotic model mice induced by spinal cord injury and sciatic nerve resection.
abstract: In the present study, we examined intraosseous blood vessel parameters of the tibial metaphysis in mice using microcomputed tomography (µCT) to investigate the relationship between post-nerve-injury osteoporosis and local intraosseous blood vessel volume and number. Mice were randomly divided into groups receiving spinal cord injury (SCI), sciatic nerve resection group (NX), or intact controls (30 mice/group). Four weeks after surgery, mice were perfused with silicone and the distribution of intraosseous blood vessels analyzed by μCT. The bone density, μCT microstructure, biomechanical properties, and the immunohistochemical and biochemical indicators of angiogenesis were also measured. The SCI group showed significantly reduced tibial metaphysis bone density, μCT bone microstructure, tibial biomechanical properties, indicators of angiogenesis, and intraosseous blood vessel parameters compared to the NX group. Furthermore, the spinal cord-injured mice exhibited significantly decreased intraosseous blood vessel volume and number during the development of osteoporosis. In conclusion, these data suggest that decreased intraosseous blood vessel volume and number may play an important role in the development of post-nerve-injury osteoporosis.
authors: Department of Orthopaedics, Third Affiliated Hospital of Suzhou University, Changzhou 213001, China.
authors: Wen-Ge
authors: WG
authors: Ding
authors: Wei-hong
authors: WH
authors: Yan
authors: Zhao-Xiang
authors: ZX
authors: Wei
authors: Jin-Bo
authors: JB
authors: Liu
mesh_terms: D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000473:pathology; D004195:Disease Models, Animal; D004838:Epiphyses / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology; D005169:Factor VIII / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000000981:diagnostic imaging / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology*; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011897:Random Allocation; D012584:Sciatic Nerve / Q000293:injuries*; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D013977:Tibia / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055114:X-Ray Microtomography
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D005169:Factor VIII