title: Efficient myogenic reprogramming of adult white fat stem cells and bone marrow stem cells by freshly isolated skeletal muscle fibers.
abstract: Stem cells that can be directed to differentiate into specific cell types offer the prospect of a renewable source of replacement cells to treat diseases. This study evaluates the reprogramming of 2 readily available stem cell populations into skeletal muscle. We show for the first time that freshly isolated muscle fibers reprogram bone marrow or white fat stem cells far more efficiently than muscle cell lines. In addition, we show that the ability of muscle fibers to reprogram stem cells can be almost doubled through the use of chromatin remodeling reagents such as trichostatin A. This novel approach permits the generation of myogenic cells that could be used to treat a range of muscle-wasting diseases.
authors: School Biological Sciences, University of Reading, Reading, UK.
authors: Henry
authors: H
authors: Collins-Hooper
authors: Graham
authors: G
authors: Luke
authors: Mark
authors: M
authors: Cranfield
authors: William R
authors: WR
authors: Otto
authors: Steve
authors: S
authors: Ray
authors: Ketan
authors: K
authors: Patel
mesh_terms: D052436:Adipose Tissue, White / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D050152:Intra-Abdominal Fat / Q000166:cytology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D009133:Muscular Atrophy / Q000473:pathology / Q000628:therapy; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar
chemical_list: D049452:Green Fluorescent Proteins