title: Left ventricular remodeling in swine after myocardial infarction: a transcriptional genomics approach.
abstract: Despite the apparent appropriateness of left ventricular (LV) remodeling following myocardial infarction (MI), it poses an independent risk factor for development of heart failure. There is a paucity of studies into the molecular mechanisms of LV remodeling in large animal species. We took an unbiased molecular approach to identify candidate transcription factors (TFs) mediating the genetic reprogramming involved in post-MI LV remodeling in swine. Left ventricular tissue was collected from remote, non-infarcted myocardium, 3 weeks after MI-induction or sham-surgery. Microarray analysis identified 285 upregulated and 278 downregulated genes (FDR < 0.05). Of these differentially expressed genes, the promoter regions of the human homologs were searched for common TF binding sites (TFBS). Eighteen TFBS were overrepresented >two-fold (p < 0.01) in upregulated and 13 in downregulated genes. Left ventricular nuclear protein extracts were assayed for DNA-binding activity by protein/DNA array. Out of 345 DNA probes, 30 showed signal intensity changes >two-fold. Five TFs were identified in both TFBS and protein/DNA array analyses, which showed matching changes for COUP-TFII and glucocorticoid receptor (GR) only. Treatment of swine with the GR antagonist mifepristone after MI reduced the post-MI increase in LV mass, but LV dilation remained unaffected. Thus, using an unbiased approach to study post-MI LV remodeling in a physiologically relevant large animal model, we identified COUP-TFII and GR as potential key mediators of post-MI remodeling.
authors: Division of Experimental Cardiology, Thoraxcenter, Erasmus MC, University Medical Center Rotterdam, P. O. Box 2040, 3000 CA Rotterdam, The Netherlands.
authors: Diederik W D
authors: DW
authors: Kuster
authors: Daphne
authors: D
authors: Merkus
authors: Andreas
authors: A
authors: Kremer
authors: Wilfred F J
authors: WF
authors: van Ijcken
authors: Vincent J
authors: VJ
authors: de Beer
authors: Adrie J M
authors: AJ
authors: Verhoeven
authors: Dirk J
authors: DJ
authors: Duncker
mesh_terms: D000818:Animals; D051837:COUP Transcription Factor II / Q000096:biosynthesis / Q000235:genetics; D004195:Disease Models, Animal; D004452:Echocardiography; D005260:Female; D020869:Gene Expression Profiling; D023281:Genomics; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis / Q000235:genetics; D013552:Swine; D014157:Transcription Factors; D020257:Ventricular Remodeling / Q000235:genetics*
chemical_list: D051837:COUP Transcription Factor II; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors