title: The effects of testosterone on intestinal ischemia/reperfusion in rats.
abstract: ABSTRACT   Ischemic injury to the gut is believed to occur in many serious clinical conditions. Our aim was to investigate the postischemia/reperfusion (I/R) effects of exogenously administered testosterone on the intestines of normal and orchiectomized rats.Forty-eight rats were divided into eight groups of six animals: (1) Sham-operated control group; (2) Sham-operated + testosterone-treated group; (3) I/R group: Rats were subjected to the surgical procedures and underwent intestinal ischemia for 60 min followed by reperfusion for 60 min; (4) I/R + testosterone-treated group: Rats were subjected to the surgical procedures and received testosterone 100 mg/kg (i.p.); (5) I/R + orchiectomy group: Rats were subjected to the surgical procedures as well as orchiectomy; (6) orchiectomy group: Rats were subjected to the surgical procedures as well as orchiectomy; (7) orchiectomy + testosterone-treated group: Rats were subjected to the surgical procedures as well as orchiectomy and received testosterone 100 mg/kg (i.p.); and (8) I/R + orchiectomy + testosterone-treated group. The histological findings of this study paralleled the observed degree of lipid peroxidation (LPO) and protein oxidation. Intestinal mucosal injury was extensive in the I/R, I/R + orchiectomy, and I/R + orchiectomy + testosterone groups, but was less in the I/R + testosterone group. Histopathological injury also paralleled the degree of oxidative stress. Apoptotic enterocytes were more numerous in the I/R, I/R + orchiectomy, and I/R + orchiectomy + testosterone groups. Administration of testosterone in the presence of testes significantly protected intestinal tissue against I/R mucosal injuries, while administration of testosterone in the absence of testes did not significantly protect intestinal tissue against I/R mucosal injuries.
authors: Department of Surgery, Education and Research Hospital, Yildizkent, Erzurum, Turkey. yavuzalbayrakdr@gmail.com
authors: Yavuz
authors: Y
authors: Albayrak
authors: Zekai
authors: Z
authors: Halici
authors: Fehmi
authors: F
authors: Odabasoglu
authors: Deniz
authors: D
authors: Unal
authors: Osman Nuri
authors: ON
authors: Keles
authors: Ismail
authors: I
authors: Malkoc
authors: Akgun
authors: A
authors: Oral
authors: Muhammed
authors: M
authors: Yayla
authors: Ozlem
authors: O
authors: Aydin
authors: Bunyami
authors: B
authors: Unal
mesh_terms: D000818:Animals; D007410:Intestinal Diseases / Q000517:prevention & control*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D009919:Orchiectomy; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control*; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D013739:Testosterone