title: Human hepatocytes: isolation, culture, and quality procedures.
abstract: The use of isolated human liver cells in research and development has gained increasing interest during the past years. The possible application may vary between elucidation of new biochemical pathways in liver diseases, drug development, safety issues, and new therapeutic strategies up to direct clinical translation for liver support. However, the isolation of human liver cells requires a well-developed logistic network among surgeons, biologists, and technicians to obtain a high quality of cells. Our laboratories have been involved in various applications of human liver cells and we have long-lasting experiences in human liver cell isolation and their application in R&D. We here summarize the present protocol of our laboratories for cell isolation from normal resected liver tissue, the most common tissue available. In addition, we discuss the necessary network in the clinic and quality controls to maintain human liver cells in culture and the effect of 3D extracellular matrix in cultured cells which results in preservation of hepatocyte epithelial polarity in the form of bile canaliculi and repression of epithelial to mesenchymal transitions occurring in 2D cultures.
authors: Cytonet Gmbh, Weinheim, Germany.
authors: Daniel
authors: D
authors: Knobeloch
authors: Sabrina
authors: S
authors: Ehnert
authors: Lilianna
authors: L
authors: Schyschka
authors: Peter
authors: P
authors: Büchler
authors: Michael
authors: M
authors: Schoenberg
authors: Jörg
authors: J
authors: Kleeff
authors: Wolfgang E
authors: WE
authors: Thasler
authors: Natascha C
authors: NC
authors: Nussler
authors: Patricio
authors: P
authors: Godoy
authors: Jan
authors: J
authors: Hengstler
authors: Andreas K
authors: AK
authors: Nussler
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D061251:Primary Cell Culture / Q000379:methods* / Q000592:standards*; D011786:Quality Control