title: Excretory/secretory antigens from Dirofilaria immitis adult worms interact with the host fibrinolytic system involving the vascular endothelium.
abstract: Dirofilaria immitis is the causative agent of canine and feline heartworm disease. The parasite can survive for long periods of time (7 years or more) in the circulatory system of immunocompetent reservoirs, producing usually a chronic inflammatory vascular disease. In addition, the simultaneous death of groups of adult worms can trigger an acute disease characterized by the exacerbation of inflammatory reactions and the emergence of serious thromboembolic events. In the context of the D. immitis/host relationships, the aim of this study was to investigate the interaction between the excretory/secretory antigens from D. immitis adult worms (DiES) and the fibrinolytic system of the host. Using an enzyme-linked immunosorbent assay we showed that DiES extract is able to bind plasminogen and generate plasmin, although this fact requires the presence of the tissue plasminogen activator (t-PA). Moreover, we established that DiES extract enhances t-PA expression in cultured vascular endothelial cells. Additionally, 10 plasminogen-binding proteins from DiES extract were identified by mass spectrometry (HSP60, actin-1/3, actin, actin 4, transglutaminase, GAPDH, Ov87, LOAG_14743, galectin and P22U). The data suggest that DiES antigens interact with the environment of the parasite regulating the activation of the fibrinolytic system of the host with involvement of the vascular endothelium in the process.
authors: Laboratory of Parasitology, University of Salamanca, Avda. Campo Charro s/n, 37007, Salamanca, Spain.
authors: Javier
authors: J
authors: González-Miguel
authors: Rodrigo
authors: R
authors: Morchón
authors: Isabel
authors: I
authors: Mellado
authors: Elena
authors: E
authors: Carretón
authors: José Alberto
authors: JA
authors: Montoya-Alonso
authors: Fernando
authors: F
authors: Simón
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism*; D002460:Cell Line; D004183:Dirofilaria immitis / Q000378:metabolism*; D004184:Dirofilariasis / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism / Q000469:parasitology*; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D006790:Host-Parasite Interactions; D006801:Humans; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding
chemical_list: D000947:Antigens, Helminth; D010958:Plasminogen; D005341:Fibrinolysin