title: In vitro antibacterial activity of different endodontic irrigants.
abstract: OBJECTIVE
  The objective of this study was to compare in vitro the antibacterial activity of Tetraclean (mixture of doxycycline, citric acid and polypropylene glycol), Niclor 5 (5.25% sodium hypochlorite solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution) and hydrogen peroxide 12 volumes on three endodontic pathogens associated with primary endodontic infections. Enterococcus faecalis, Streptococcus mutans and Staphylococcus aureus strains were selected to evaluate the antibacterial activity of endodontic irrigants by the agar disc-diffusion test.
  
  
  METHODS
  Paper discs were saturated with each one of the test solutions (at room temperature and preheating at 50°C) and placed onto culture agar-plates preadsorbed with bacterial cells and further incubated for 24 h at 37°C. The growth inhibition zones around each irrigant were recorded and compared for each bacterial strain. Kruskal-Wallis and Mann-Whitney tests were applied to compare the various groups.
  
  
  RESULTS
  At room temperature, Tetraclean showed significantly higher inhibition of bacteria growth than all other irrigants tested. Preheating at 50°C significantly increased growth inhibition for all the groups tested. At 50°C, hydrogen peroxide 12 volumes and Tetraclean showed significantly higher efficacy than all other irrigants tested.
  
  
  CONCLUSIONS
  50°C-preheated hydrogen peroxide 12 volumes and Tetraclean showed highest inhibition of the bacterial growth.
authors: Department of Operative Dentistry, University of Pavia, Piazzale Golgi 3, Pavia, Italy. claudio.poggio@unipv.it
authors: Claudio
authors: C
authors: Poggio
authors: Marco
authors: M
authors: Colombo
authors: Andrea
authors: A
authors: Scribante
authors: Dario
authors: D
authors: Sforza
authors: Stefano
authors: S
authors: Bianchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D006361:Heating; D008826:Microbial Sensitivity Tests; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants