title: Embryo quality and transcervical technique are not the limiting factors in donkey embryo transfer outcome.
abstract: Embryo transfer (ET) in the donkey resulted in a very low recipient pregnancy rates. The aim of these studies was to investigate if nonsurgical transfer techniques or donkey embryo quality affect donkey recipient pregnancy failure. In Study 1, the impact of transfer technique was investigated by evaluating if cervical catheterization is associated with prostaglandin release and suppression of luteal function and if donkey recipients would become pregnant after nonsurgical transfer of horse embryos. Four jennies, from 5 to 8 d after ovulation, were submitted to a sham transcervical ET and to evaluation of PGFM and progesterone plasma concentrations. Five 8 d horse embryos were nonsurgically transferred into synchronized donkey recipients (HD). Cervical stimulation caused a transient PGF(2α) release in two of four jennies in the absence of a significant decrease in progesterone plasma concentration. All transferred horse embryos resulted in pregnancies in the jenny recipients. In Study 2, donkey embryo viability was investigated by 1.2 meters, 6-diamidino-2-phenylindole (DAPI) staining of 10 embryos and by the transfer of 6 and 12 donkey embryos in synchronized mare (DH) and donkey (DD) recipients, respectively, of known fertility. The estimated proportion of dead cells in DAPI stained embryos was 0.9% (range 0-3.9%) and below what is considered normal (20%) for horse embryos. Three of six and six of 12 of the DH and DD ETs, respectively resulted in pregnancies at 14 and 25 d (50%), a higher pregnancy rate than previously reported after DD ET. The overall results of this study suggest that the transcervical technique for ET and donkey embryo viability are not the reasons for the low pregnancy rates that have previously been described in donkey recipients, and that nonsurgical ET in donkeys can result in acceptable results.
authors: Dipartimento di Clinica Veterinaria, Università degli Studi di Pisa, Italy. d.panzani@vet.unipi.it
authors: D
authors: D
authors: Panzani
authors: A
authors: A
authors: Rota
authors: A
authors: A
authors: Crisci
authors: H
authors: H
authors: Kindahl
authors: N
authors: N
authors: Govoni
authors: F
authors: F
authors: Camillo
mesh_terms: D000818:Animals; D004624:Embryo Transfer / Q000662:veterinary*; D018397:Equidae / Q000502:physiology*; D005260:Female; D007315:Insemination, Artificial; D011247:Pregnancy; D013045:Species Specificity; D013997:Time Factors