title: The Pseudomonas aeruginosa toxin L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth and induces encystment in Acanthamoeba castellanii.
abstract: L-2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a toxic antimetabolite produced by the opportunistic pathogen Pseudomonas aeruginosa. To evaluate its importance as a potential virulence factor, we tested the host response towards AMB using an Acanthamoeba castellanii cell model. We found that AMB (at concentrations ≥ 0.5 mM) caused amoebal encystment in salt buffer, while inhibiting amoebal growth in rich medium in a dose-dependent manner. However, no difference in amoebal plaque formation was observed on bacterial lawns of wild type and AMB-negative P. aeruginosa strains. We thereby conclude that AMB may eventually act as a virulence factor, but only at relatively high concentrations.
authors: Département de Microbiologie Fondamentale, Université de Lausanne, Bâtiment Biophore, CH-1015 Lausanne, Switzerland.
authors: Xiaoyun
authors: X
authors: Lee
authors: Cornelia
authors: C
authors: Reimmann
authors: Gilbert
authors: G
authors: Greub
authors: Janice
authors: J
authors: Sufrin
authors: Antony
authors: A
authors: Croxatto
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects* / Q000254:growth & development* / Q000502:physiology; D000613:Aminobutyrates / Q000737:chemistry / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000737:chemistry / Q000494:pharmacology; D002021:Buffers; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D050296:Microbial Viability / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D013194:Staining and Labeling; D053058:Trophozoites / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D014343:Trypan Blue / Q000737:chemistry; D037521:Virulence Factors / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000613:Aminobutyrates; D001427:Bacterial Toxins; D002021:Buffers; D003470:Culture Media; D037521:Virulence Factors; C000264:2-amino-4-methoxy-3-butenoic acid; C006507:vinylglycine; D014343:Trypan Blue; D005998:Glycine