title: Characterization of a novel biosurfactant produced by Staphylococcus sp. strain 1E with potential application on hydrocarbon bioremediation.
abstract: A biosurfactant-producing bacterium (Staphylococcus sp. strain 1E) was isolated from an Algerian crude oil contaminated soil. Biosurfactant production was tested with different carbon sources using the surface tension measurement and the oil displacement test. Olive oil produced the highest reduction in surface tension (25.9 dynes cm(-1)). Crude oil presented the best substrate for 1E biosurfactant emulsification activity. The biosurfactant produced by strain 1E reduced the growth medium surface tension below 30 dynes cm(-1). This reduction was also obtained in cell-free filtrates. Biosurfactant produced by strain 1E showed stability in a wide range of pH (from 2 to 12), temperature (from 4 to 55 °C) and salinity (from 0 to 300 g l(-1)) variations. The biosurfactant produced by strain 1E belonged to lipopeptide group and also constituted an antibacterial activity againt the pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis. Phenanthrene solubility in water was enhanced by biosurfactant addition. Our results suggest that the 1E biosurfactant has interesting properties for its application in bioremediation of hydrocarbons contaminated sites.
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional AUF PER-LBPE, Centre de Biotechnologie de Sfax, Tunisie.
authors: Kamel
authors: K
authors: Eddouaouda
authors: Sami
authors: S
authors: Mnif
authors: Abdelmalek
authors: A
authors: Badis
authors: Sonia Ben
authors: SB
authors: Younes
authors: Slim
authors: S
authors: Cherif
authors: Samira
authors: S
authors: Ferhat
authors: Najla
authors: N
authors: Mhiri
authors: Mohamed
authors: M
authors: Chamkha
authors: Sami
authors: S
authors: Sayadi
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004655:Emulsions / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D006838:Hydrocarbons / Q000378:metabolism*; D055666:Lipopeptides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D010578:Petroleum / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013210:Staphylococcus / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013501:Surface-Active Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004655:Emulsions; D006838:Hydrocarbons; D055666:Lipopeptides; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013501:Surface-Active Agents