title: Screening method for isopeptides from small ubiquitin-related modifier-conjugated proteins by ion mobility mass spectrometry.
abstract: Posttranslational modification by the small ubiquitin-related modifier (SUMO) is a highly regulated modification, which is often restricted to very specific cellular events. A number of analytical strategies for identification of SUMOylated proteins have been previously reported in the literature. A new screening method for SUMOylated peptides based on ion mobility mass spectrometry is presented. Using poly-SUMO2 as a model system, a two-enzyme trypsin/chymotrypsin digestion was performed to reduce the size of the isopeptide conjugated to the substrate lysine residue. Traveling wave ion mobility mass spectrometry was used to screen for peptides containing the QQQTGG isopeptide tag from SUMO, which increases the mass and size of the peptide by 618 Da. This increase in mass along with solution conditions to promote higher charge states allows the isopeptides to be separated from the typically smaller and lesser charged linear peptides. On the basis of these findings, this method can be used as a quick and easy screening method for identifying possible SUMO isopeptides.
authors: Department of Chemistry, University of Toledo, Toledo, Ohio 43606, United States.
authors: Quentin
authors: Q
authors: Dumont
authors: David L
authors: DL
authors: Donaldson
authors: Wendell P
authors: WP
authors: Griffith
mesh_terms: D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000378:metabolism; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010455:Peptides; D025841:Small Ubiquitin-Related Modifier Proteins; D002918:Chymotrypsin; D014357:Trypsin