title: Molecular cloning, characterization expression of P450c17-I and P450c17-II and their functions analysis during the reproductive cycle in males of barfin flounder (Verasper moseri).
abstract: P450c17, a key steroidogenic enzyme, plays important roles in the production of sex steroid and cortisol. In teleost, there are two types of P450c17, P450c17-I possessing 17α-hydroxylase and 17, 20-lyase activities, and P450c17-II only possessing 17α-hydroxylase activity. This work describes the molecular cloning of the cDNA encoding the barfin flounder (Verasper moseri) P450c17-I and P450c17-II by means of RT-PCR and 5' and 3' rapid amplification of cDNA ends (RACE) analyses and mRNA expression profiles analyzing by semiquantitative RT-PCR. Respectively, P450c17-I and P450c17-II mRNA levels in the testes correlated with serum testosterone (T) level, as well as gonadosomatic index (GSI) of males during specific stages of spermatogenesis. P450c17-I and P450c17-II mRNA were expressed in the testis and ovary, suggesting that both of them participate in the production of sex steroid in barfin flounder gonads. P450c17-I mRNA was undetectable; in contrast, P450c17-II mRNA was detected at the highest level in the head kidney, meaning that only P450c17-II is involved in the production of cortisol in barfin flounder. The results demonstrated that both of P450c17-I and P450c17-II participate in the production of sex steroid in male barfin flounder gonads.
authors: Fisheries College, Ocean University of China, 5 Yushan Road, Qingdao, China.
authors: Guo X
authors: GX
authors: Jin
authors: Hai S
authors: HS
authors: Wen
authors: Feng
authors: F
authors: He
authors: Ji F
authors: JF
authors: Li
authors: Cai F
authors: CF
authors: Chen
authors: Jia R
authors: JR
authors: Zhang
authors: Xiao Y
authors: XY
authors: Chen
authors: Bao
authors: B
authors: Shi
authors: Dan
authors: D
authors: Shi
authors: Yan P
authors: YP
authors: Yang
authors: Bao X
authors: BX
authors: Qi
authors: Na
authors: N
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005432:Flounder / Q000235:genetics* / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism*; D013737:Testis / Q000201:enzymology; D013739:Testosterone / Q000097:blood
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D013739:Testosterone; D013254:Steroid 17-alpha-Hydroxylase