title: CCL28 induces mucosal homing of HIV-1-specific IgA-secreting plasma cells in mice immunized with HIV-1 virus-like particles.
abstract: Mucosae-associated epithelial chemokine (MEC or CCL28) binds to CCR3 and CCR10 and recruits IgA-secreting plasma cells (IgA-ASCs) in the mucosal lamina propria. The ability of this chemokine to enhance migration of IgA-ASCs to mucosal sites was assessed in a mouse immunization model using HIV-1(IIIB) Virus-like particles (VLPs). Mice receiving either HIV-1(IIIB) VLPs alone, CCL28 alone, or the irrelevant CCL19 chemokine were used as controls. Results showed a significantly increased CCR3 and CCR10 expression on CD19(+) splenocytes of HIV-1(IIIB) VPL-CCL28-treated mice. HIV-1 Env-specific IFN-γ, IL-4 and IL-5 production, total IgA, anti-Env IgA as well as gastro-intestinal mucosal IgA-secreting plasma cells were also significantly augmented in these mice. Notably, sera and vaginal secretions from HIV-1(IIIB) VLP-CCL28-treated mice exhibited an enhanced neutralizing activity against both a HIV-1/B-subtype laboratory strain and a heterologous HIV-1/C-subtype primary isolate. These data suggest that CCL28 could be useful in enhancing the IgA immune response that will likely play a pivotal role in prophylactic HIV vaccines.
authors: Department of Clinical Sciences, University of Milan, Milan, Italy. veronica.rainone@unimi.it
authors: Veronica
authors: V
authors: Rainone
authors: Gregor
authors: G
authors: Dubois
authors: Vladimir
authors: V
authors: Temchura
authors: Klaus
authors: K
authors: Überla
authors: Alberto
authors: A
authors: Clivio
authors: Manuela
authors: M
authors: Nebuloni
authors: Eleonora
authors: E
authors: Lauri
authors: Daria
authors: D
authors: Trabattoni
authors: Francisco
authors: F
authors: Veas
authors: Mario
authors: M
authors: Clerici
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D054415:Chemokine CCL19 / Q000378:metabolism; D019742:Chemokines, CC / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D051379:Mice; D009500:Neutralization Tests; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D054447:Receptors, CCR10 / Q000378:metabolism; D054397:Receptors, CCR3 / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D014771:Virion / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D018941:Antigens, CD19; C515461:Ccl19 protein, mouse; C511142:Ccl28 protein, mouse; C515707:Ccr10 protein, mouse; C515351:Ccr3 protein, mouse; D054415:Chemokine CCL19; D019742:Chemokines, CC; D007071:Immunoglobulin A, Secretory; D054447:Receptors, CCR10; D054397:Receptors, CCR3; D011994:Recombinant Proteins; D054299:env Gene Products, Human Immunodeficiency Virus