title: The roles of the proteasome, and cathepsins B, L, H and D, in ostrich meat tenderisation.
abstract: As very little research has been conducted on ostrich meat tenderisation, this study aims at investigating the roles of the proteasome and cathepsins B, L, H, and D in the tenderisation process. The enzyme activities in meat from eight ostriches during a 12-day ageing period and the corresponding physical characteristics (e.g. pH, shear force) and myofibril patterns were determined. After 12 days, substantial high remaining activities were found, especially of the proteasome, thus implicating their possible roles in the tenderisation process. The mean shear force values, however, showed no improvement in tenderness, but the myofibril patterns showed the appearance of a M(r) 32 K component. Myofibril degradation studies of the proteasome, analysed electrophoretically, also revealed a possible role of the proteasome, but under activating conditions. This study provides further insights into the tenderisation process, particularly of ostrich meat, which may ultimately be used for the advantageous manipulation of the process.
authors: Department of Biochemistry and Microbiology, University of Port Elizabeth, P.O. Box 1600, Port Elizabeth 6000, South Africa.
authors: Adele R
authors: AR
authors: Thomas
authors: Hatizivi
authors: H
authors: Gondoza
authors: Louwrens C
authors: LC
authors: Hoffman
authors: Vaughan
authors: V
authors: Oosthuizen
authors: Ryno J
authors: RJ
authors: Naudé