title: Functional analysis of the cyclopiazonic acid biosynthesis gene cluster in Aspergillus oryzae RIB 40.
abstract: The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cyclo-acetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.
authors: Noda Institute for Scientific Research, Noda, Chiba, Japan. yshinohara@mail.kikkoman.co.jp
authors: Yasutomo
authors: Y
authors: Shinohara
authors: Masafumi
authors: M
authors: Tokuoka
authors: Yasuji
authors: Y
authors: Koyama
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics / Q000378:metabolism*; D007211:Indoles / Q000138:chemical synthesis* / Q000378:metabolism*; D005810:Multigene Family; D009183:Mycotoxins / Q000096:biosynthesis* / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: C570740:2-oxocyclopiazonic acid; D007211:Indoles; D009183:Mycotoxins; C570739:cyclo-acetoacetyl-L-tryptophan; D048630:Polyketide Synthases; D014364:Tryptophan; C000543:cyclopiazonic acid