title: Proteomic analysis and protein carbonylation profile in trained and untrained rat muscles.
abstract: Understanding the relationship between physical exercise, reactive oxygen species and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Unbalanced ROS levels can lead to oxidation of cellular macromolecules and a major class of protein oxidative modification is carbonylation. The aim of this investigation was to study muscle protein expression and carbonylation patterns in trained and untrained animal models. We analyzed two muscles characterized by different metabolisms: tibialis anterior and soleus. Whilst tibialis anterior is mostly composed of fast-twitch fibers, the soleus muscle is mostly composed of slow-twitch fibers. By a proteomic approach we identified 15 protein spots whose expression is influenced by training. Among them in tibialis anterior we observed a down-regulation of several glycolitic enzymes. Concerning carbonylation, we observed the existence of a high basal level of protein carbonylation. Although this level shows some variation among individual animals, several proteins (mostly involved in energy metabolism, muscle contraction, and stress response) appear carbonylated in all animals and in both types of skeletal muscle. Moreover we identified 13 spots whose carbonylation increases after training.
authors: Department of Biochemistry, University of Florence, Florence, Italy. francesca.magherini@unifi.it
authors: Francesca
authors: F
authors: Magherini
authors: Provvidenza Maria
authors: PM
authors: Abruzzo
authors: Michele
authors: M
authors: Puglia
authors: Luca
authors: L
authors: Bini
authors: Tania
authors: T
authors: Gamberi
authors: Fabio
authors: F
authors: Esposito
authors: Arsenio
authors: A
authors: Veicsteinas
authors: Marina
authors: M
authors: Marini
authors: Claudia
authors: C
authors: Fiorillo
authors: Massimo
authors: M
authors: Gulisano
authors: Alessandra
authors: A
authors: Modesti
mesh_terms: D000818:Animals; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D050050:Protein Carbonylation / Q000502:physiology*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009124:Muscle Proteins; D020543:Proteome