title: [Antibody responses induced by mucosal DNA vaccine encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium].
abstract: OBJECTIVE
  In order to investigate antibody responses by musosal DNA vaccines encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium aroA strain SL7207 (SL7207) via intranasal or intragastric routes.
  
  
  METHODS
  After the codon-optimized F gene was synthesized, we constructed eukaryotic expression plasmid pcDNA3.1/Fsyn and transformed it into SL7207 to get recombinant SL7207 of SL7207/pcDNA3.1/Fsyn. Then, SL7207/pcDNA3.1/Fsyn was exploited to evaluate its immunogenicity in BALB/c mice administered intranasally or intragastrically. The resultant serum and mucosal antibody responses were analyzed by indirect ELISA.
  
  
  RESULTS
  Compared with intragastric immunization group, intranasal immunization group achieved higher level of RSV specific serum IgG and secretion IgA (SIgA) (P < 0.05). Moreover, F protein expressed by the codon-optimized F gene of Fsyn was of elevated immunogenicity, compared with wild type F (Fwt) (P < 0.05).
  
  
  CONCLUSIONS
  Intranasal immunization and codon optimization approaches can improve antibody responses of RSV DNA vaccine delivered by attenuated Salmonella typhimurium aroA strain SL7207.
authors: Department of Immunology, Anhui Medical University, Hefei 230032, China.
authors: Ming
authors: M
authors: Cheng
authors: Jinsheng
authors: J
authors: He
authors: Yuanhui
authors: Y
authors: Fu
authors: Wei
authors: W
authors: Qiao
authors: Yueying
authors: Y
authors: Jiao
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D003062:Codon; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D003062:Codon; C526850:F protein, human respiratory syncytial virus; D022261:Respiratory Syncytial Virus Vaccines; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014760:Viral Fusion Proteins