title: Interaction between Pax6 and its novel mutant in Bufo raddei Strauch.
abstract: OBJECTIVE
  Exploration of the relationship between a novel paired box 6 (Pax6) mutant and Pax6 in Bufo raddei Strauch.
  
  
  METHODS
  RT-PCR, yeast 2-hybrid system, and co-immunoprecipitation were used to analyze the Pax6 protein and its mutant during embryonic eye development in Bufo raddei Strauch.
  
  
  RESULTS
  We have cloned the Pax6 ORF sequence from Bufo raddei Strauch. Here we report the cloning of a novel Pax6 homolog of Bufo raddei Strauch named Pax6 variant. Comparing the 2 genes, the homolog of ORF nucleotide sequence is more than 99% in Bufo raddei Strauch; only the proline-serine-threonine(PST)-rich transaction domain differs. The deduced amino acid sequences of PST region are 53.1% identical. An interaction was found between Pax6 and Pax6 variant via yeast 2-hybrid system; with further study, we found that they interacted in vivo via co-immunopricipitation.
  
  
  CONCLUSIONS
  A Pax6 mutant was first found in Bufo raddei Strauch. Interaction between Pax6 and Pax6 variant may play a critical role during eye development in Bufo raddei Strauch. This suggests that expression of Pax6 variant may play a role and appears to be a necessity in eye development, but that Pax6 itself is still pivotal in eye development.
authors: School of Life Science, Lanzhou University, Gansu, China.
authors: Furong
authors: F
authors: Ju
authors: Yongqing
authors: Y
authors: Zhao
authors: Yuanlin
authors: Y
authors: Zhao
authors: Ying
authors: Y
authors: Wang
authors: Fan
authors: F
authors: Wen
authors: Lin
authors: L
authors: Ye
authors: Lan
authors: L
authors: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002025:Bufonidae / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D009154:Mutation; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation; D014169:Transformation, Bacterial; D020798:Two-Hybrid System Techniques
chemical_list: D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D020033:Protein Isoforms; D011994:Recombinant Proteins; D012097:Repressor Proteins