title: Investigation of the microbial ecology of Ciauscolo, a traditional Italian salami, by culture-dependent techniques and PCR-DGGE.
abstract: The microbial ecology of 22 samples of commercially available Ciauscolo salami were investigated using a polyphasic approach, based on culture-dependent and -independent techniques. The viable counts of pathogen and hygiene indicator microorganisms highlighted the adequate application of good manufacturing practices, while the viable counts of the lactic acid bacteria, coagulase negative cocci, and yeasts showed dominance of the first of these microbial groups. Bacterial and fungal DNA were extracted directly from the salami and amplified by PCR, using two primer sets targeting the 16S and 28S rRNA genes, respectively. Denaturing gradient gel electrophoresis (DGGE) and sequencing of selected bands were used to investigate the microbial ecology of these Ciauscolo salami. The most frequently found bacterial species were Lactobacillus sakei and Lb. curvatus, while Debaryomyces hansenii was the prevalent yeast species detected. Cluster analysis of the DGGE profiles and calculation of biodiversity indices allowed the degree of microbial similarity across these salami to be determined.
authors: Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, via Brecce Bianche, 60131 Ancona, Italy.
authors: Gloria
authors: G
authors: Silvestri
authors: Sara
authors: S
authors: Santarelli
authors: Lucia
authors: L
authors: Aquilanti
authors: Alessandra
authors: A
authors: Beccaceci
authors: Andrea
authors: A
authors: Osimani
authors: Franco
authors: F
authors: Tonucci
authors: Francesca
authors: F
authors: Clementi