title: Vaccination of lactating dairy cows for the prevention of aflatoxin B1 carry over in milk.
abstract: The potential of anaflatoxin B(1) (AnAFB(1)) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB(1)-KLH) in controlling the carry over of the aflatoxin B(1) (AFB(1)) metabolite aflatoxin M(1) (AFM(1)) in cow milk is reported. AFB(1) is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB(1) is AFB(1) chemically modified as AFB(1)-1(O-carboxymethyl) oxime. In comparison to AFB(1), AnAFB(1) has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB(1)-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB(1) IgG antibodies (Abs) which were cross reactive with AFB(1), AFG(1), and AFG(2). The elicited anti-AFB(1) Abs were able to hinder the secretion of AFM(1) into the milk of cows continuously fed with AFB(1). Vaccination of lactating animals with conjugated AnAFB(1) may represent a solution to the public hazard constituted by milk and cheese contaminated with AFs.
authors: Sezione di Microbiologia, Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi, Parma, Italy. luciano.polonelli@unipr.it
authors: Luciano
authors: L
authors: Polonelli
authors: Laura
authors: L
authors: Giovati
authors: Walter
authors: W
authors: Magliani
authors: Stefania
authors: S
authors: Conti
authors: Stefano
authors: S
authors: Sforza
authors: Alessandro
authors: A
authors: Calabretta
authors: Claudio
authors: C
authors: Casoli
authors: Paola
authors: P
authors: Ronzi
authors: Ester
authors: E
authors: Grilli
authors: Antonio
authors: A
authors: Gallo
authors: Francesco
authors: F
authors: Masoero
authors: Gianfranco
authors: G
authors: Piva
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology / Q000633:toxicity; D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003429:Cross Reactions / Q000276:immunology; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007106:Immune Sera / Q000187:drug effects; D007774:Lactation / Q000187:drug effects / Q000502:physiology*; D008892:Milk / Q000737:chemistry*; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014611:Vaccination
chemical_list: D000908:Antibodies, Fungal; D007106:Immune Sera; D016604:Aflatoxin B1