title: Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition.
abstract: To improve the arachidonic acid (ARA) accumulation in Mortierella alpina, a mixed B-group vitamin addition strategy was developed. The ARA titer reached up to 10.0 g/L, 1.7-fold of the control. At the same time, the highest specific activities of key enzymes involved in ARA biosynthesis, including malic enzyme, glucose-6-phosphate dehydrogenase and ATP: citrate lyase, were 63.3, 38.6 and 53.7% higher than the control, respectively. The possible vitamin triggered improved ARA accumulation mechanism was thus elucidated that B-group vitamins could function as the cofactors of the key enzymes involved in ARA biosynthesis, or precursors for the formation of NADPH and acetyl-CoA which were crucial for ARA synthesis, and strengthened the related metabolic flux.
authors: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, People's Republic of China.
authors: Yan
authors: Y
authors: Zeng
authors: Xiao-Jun
authors: XJ
authors: Ji
authors: Shu-Mei
authors: SM
authors: Chang
authors: Zhi-Kui
authors: ZK
authors: Nie
authors: He
authors: H
authors: Huang
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D016718:Arachidonic Acid / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D020094:Mortierella / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000096:biosynthesis; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis; D014803:Vitamin B Complex / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D009097:Multienzyme Complexes; D014803:Vitamin B Complex; D016718:Arachidonic Acid; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D007652:Oxo-Acid-Lyases; C019909:citrate (pro-3S)-lyase