title: Modulation of the heterogeneous senescence of human mesenchymal stem cells on chemically-modified surfaces.
abstract: Human mesenchymal stem cells (hMSCs) are multipotent and have been recognized as a source for tissue engineering or cell therapy. It is, therefore, imperative to develop methods to acquire enough hMSCs that maintain self-renewal and differentiation potential. However, aged hMSCs are prone to have a gradual decline in differentiation and proliferation potential with continual cell cycle divisions during in vitro culture. The physiochemical properties of hMSCs are highly dependent on their micro-environment, i.e. the 'stem cell niche'. In this study, the heterogeneous aging of hMSC was examined on chemically defined self-assembly monolayer surfaces. Surface energy was shown to regulate aged hMSC morphology, survival, and proteoglycan expression. High surface energy supplied a preferable environment for hMSC survival and expression of proteoglycans. These results will prove valuable to the design of scaffolds for tissue engineering or for the modulation of implantation environments.
authors: Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
authors: Sung Hoon
authors: SH
authors: Kim
authors: Byung Man
authors: BM
authors: Lee
authors: Seul Ki
authors: SK
authors: Min
authors: Sun U
authors: SU
authors: Song
authors: Jeong Ho
authors: JH
authors: Cho
authors: Kilwon
authors: K
authors: Cho
authors: Hwa Sung
authors: HS
authors: Shin
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D016922:Cellular Senescence; D005898:Glass / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D008858:Microscopy, Phase-Contrast; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D012821:Silanes / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011509:Proteoglycans; D012821:Silanes