title: Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature.
abstract: In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.
authors: Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech-Wake Forest University, Blacksburg, VA 24061, USA.
authors: Alireza
authors: A
authors: Salmanzadeh
authors: Lina
authors: L
authors: Romero
authors: Hadi
authors: H
authors: Shafiee
authors: Roberto C
authors: RC
authors: Gallo-Villanueva
authors: Mark A
authors: MA
authors: Stremler
authors: Scott D
authors: SD
authors: Cramer
authors: Rafael V
authors: RV
authors: Davalos
mesh_terms: D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival; D003198:Computer Simulation; D004586:Electrophoresis / Q000295:instrumentation*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D014411:Neoplastic Stem Cells / Q000737:chemistry* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D015203:Reproducibility of Results; D018874:Spheroids, Cellular / Q000737:chemistry / Q000166:cytology; D014407:Tumor Cells, Cultured