title: Phylogenetic and evolutionary analysis of A-, B-, C- and E-class MADS-box genes in the basal eudicot Platanus acerifolia.
abstract: London plane tree (Platanus acerifolia Willd.) is an important member of the Platanaceae family, being popular as an urban landscaping tree. Here, we report the isolation of five MADS-box genes from the basal angiosperm, Platanus acerifolia. Sequence and phylogenetic analyses identified FRUITFUL-like, APETELA3-like, AGAMOUS-like, SEPALLATA1-like and SEPALLATA3-like sequences and, hence, we term the respective Platanus acerifolia genes as PlacFUL, PlacAP3, PlacAG, PlacSEP1 and PlacSEP3. From these identities we infer that they represent candidate A-, B-, C-class and two E-class genes, respectively. The conserved MIK or MIKC domains from the nucleotide and protein sequences of PlacFUL, PlacAP3, PlacAG, PlacSEP1 and PlacSEP3 were analyzed using the maximum-likelihood, MrBayes and neighbor-joining methods. The results confirmed P. acerifolia as a basal eudicot. Expression pattern was determined by reverse transcriptase PCR, which showed all paralogous genes have distinct expression patterns, suggesting that they had undergone functional divergence.
authors: Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Zhineng
authors: Z
authors: Li
authors: Jiaqi
authors: J
authors: Zhang
authors: Guofeng
authors: G
authors: Liu
authors: Xiaomei
authors: X
authors: Li
authors: Chen
authors: C
authors: Lu
authors: Junwei
authors: J
authors: Zhang
authors: Manzhu
authors: M
authors: Bao
mesh_terms: D000595:Amino Acid Sequence; D005075:Biological Evolution; D002681:China; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D026161:MADS Domain Proteins / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D019684:Magnoliopsida / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D014197:Trees / Q000235:genetics*
chemical_list: D026161:MADS Domain Proteins