title: [Study on preparation of salidroside and polysaccharide in Rhodiola crenulata].
abstract: OBJECTIVE
  To optimize the preparation process of salidroside and polysaccharide in Rhodiola crenulata.
  
  
  METHODS
  Water-extraction and alcohol-precipitation was used. The effect of added water volume, decoction time and frequency on water-extracting process was investigated by L9 (3(4)) orthogonal design using extraction rate, content of salidroside and polysaccharide as the assessment indexes. The effect of liquor strength and ethanol concentration on alcohol-precipitating process was investigated as well, using content of salidroside and polysaccharide as the assessment indexes.
  
  
  RESULTS
  The optimum conditions for water-extracting process were as follows: decocting 3 times, and each time adding 8 fold of water and extracting 2 h. For alcohol-precipitating process,it was that liquor strength was 0.4 g/mL, and ethanol concentration in liquor was 70%.
  
  
  CONCLUSIONS
  The optimized process has guidance for the development and comprehensive utilization of this plant.
authors: Lanzhou Institute of Liver Disease, the No. 2 People's Hospital of Lanzhou, Lanzhou 730046, China. wcq.666@163.com
authors: Cong-qing
authors: CQ
authors: Wang
authors: Juan
authors: J
authors: Chen
authors: Cai-dong
authors: CD
authors: Li
authors: Bin
authors: B
authors: Wu
mesh_terms: D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*; D029771:Rhodiola / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; D011134:Polysaccharides; D014867:Water; D000431:Ethanol; C009172:rhodioloside