title: Oxygen plasma treated interactive polycarbonate DNA microarraying platform.
abstract: A novel DNA microarrying platform based on oxygen plasma activation of polycarbonate surface of compact disks (DVD) is presented. Carboxylic acid groups are generated in few seconds on polycarbonate in an efficient, fast, and clean way. Following this surface activation strategy, amino-modified oligonucleotide probes were covalently attached, reaching an immobilization density of 2 pmol cm(-2). Atomic force microscopy imaging revealed the nondestructive character of this treatment when applied for short times, allowing for disk scanning in standard DVD drives. DNA assays performed on oxygen plasma treated disks resulted very efficient with maximum hybridization yield of 93% and reaching a low limit of detection (200 pM) for perfect match synthetic oligonucleotide targets when reading the disk with a standard drive as detector. The approach was also evaluated by scoring single nucleotide polymorphisms with a discrimination ratio of 12.8. As proof of concept, the oxygen plasma treated interactive polycarbonate DNA microarraying platform was applied to the detection of PCR products of Salmonella spp., reaching a detection limit of 2 nM that corresponds to a DNA concentration of only 1 c.f.u./mL. The results confirm the suitability of the microarray platform for analysis of biological samples with high sensitivity.
authors: Departamento de Química, Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Universidad Politécnica de Valencia, Valencia, Spain.
authors: Jesús
authors: J
authors: Tamarit-López
authors: Sergi
authors: S
authors: Morais
authors: Rosa
authors: R
authors: Puchades
authors: Angel
authors: A
authors: Maquieira
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D004867:Equipment Design; D057230:Limit of Detection; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D010100:Oxygen / Q000737:chemistry*; D011076:Polycarboxylate Cement / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000235:genetics*; D013499:Surface Properties
chemical_list: D004269:DNA, Bacterial; D011076:Polycarboxylate Cement; C005506:polycarbonate; D010100:Oxygen