title: [Hydrolysis method for whey protein and the hypoglycemic activity of its peptides in diabetic KKAy mice].
abstract: OBJECTIVE
  To explore the hydrolysis method for whey protein with alkaline proteinase, and to investigate the hypoglycemic activity of the peptides in diabetic KKAy mice.
  
  
  METHODS
  Whey protein was hydrolyzed with alkaline proteinase, and the degree of hydrolysis, nitrogen and amino acid content, mass spectrographic analysis of the peptides were determined. Changes of fasting blood glucose, random blood glucose, glucose tolerance and insulin levels in diabetic KKAy mice were measured after the peptides were given by gavage.
  
  
  RESULTS
  The degree of hydrolysis of whey protein was 14.8%, the nitrogen in the peptides was 68.2%, and the molecular weight of the peptides was 900 - 1900 Dal, mostly was 1800 - 1900 Dal. Blood glucose of KKAy mice was decreased at 0.5 and 1 h after oral injection of the peptides, and insulin level was increased at 0.5 h after ingestion of the peptides.
  
  
  CONCLUSIONS
  Fasting blood glucose and random blood glucose levels of type 2 diabetic KKAy mice could be significantly decreased, and the glucose tolerance and insulin secretion of mice could be improved by whey protein peptides.
authors: Department of Nutrition, General Hospital of Chinese PLA, Beijing 100853, China. aqing_930@163.com
authors: Qing
authors: Q
authors: Xu
authors: Jin
authors: J
authors: Wang
authors: Yinghua
authors: Y
authors: Liu
authors: Changyong
authors: C
authors: Xue
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy; D005260:Female; D006868:Hydrolysis; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D051379:Mice; D008894:Milk Proteins / Q000737:chemistry / Q000627:therapeutic use*; D010455:Peptides / Q000627:therapeutic use*; D000067816:Whey Proteins
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D008894:Milk Proteins; D010455:Peptides; D000067816:Whey Proteins