title: Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells.
abstract: Artificial extracellular matrix (ECM) proteins have been designed that have strong cell-adhesive activity and active functional units that inhibit network formation among vascular endothelial cells. A laminin-derived sequence (YIGSR) that blocks migration of vascular endothelial cells was designed to incorporate into an elastin-derived structural unit. The designed ECM fusion protein also had a cell-adhesive RGDN sequence that conferred an intense migration-inhibitory effect. The resultant ECM showed cell-adhesive activity superior to that of the synthetic YIGSR peptide and it blocked the migration of vascular endothelial cells. Furthermore, the designed ECM inhibited the angiogenic activity of a collagen gel. The engineering strategy of designing multi-functional ECM proteins could be applied to support novel tissue engineering techniques.
authors: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan. maknak@toyaku.ac.jp
authors: Makiko
authors: M
authors: Nakamura
authors: Masayasu
authors: M
authors: Mie
authors: Makoto
authors: M
authors: Nakamura
authors: Eiry
authors: E
authors: Kobatake
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D023822:Tissue Engineering
chemical_list: D020533:Angiogenesis Inhibitors; D007797:Laminin; D003094:Collagen