title: Impacts of age and gender on bone marrow profiles of BMP7, BMPRs and Stro-1⁺ cells in patients with total hip replacement.
abstract: OBJECTIVE
  A successful osseointegration relies on the interplay of implant surface and surrounding bone marrow cells. This study was undertaken to investigate the impact of age and gender on the bone marrow composition.
  
  
  METHODS
  Bone marrow aspirates were obtained from the discarded metaphysis region of the femoral head in 24 patients with total hip replacement. Flow cytometry was used to measure the expression of Stro-1(+) cells and BMP receptors (BMPRs)-expressing cells. ELISA was used to measure bone marrow aspirate bone morphology protein7 (BMP7) concentration.
  
  
  RESULTS
  Our data demonstrates that there are diverse bone marrow profiles (Stro-1(+) cell and BMPRs(+) cells). There are no differences of Stro-1(+) cells, BMPRs(+) cells, and BMP7 concentration between male and female patients. Though there are slight increases in the number of Stro-1(+) cells and BMPRs(+) cells in younger patients (<70 years old) than those of old patients (≥ 70 years old), the difference is not statistically significant. However, we found a close association between the Stro-1(+) cells, BMPR1a cells and BMP7 concentration. In addition, a correlation exists between the number of Stro-1(+) cells and BMIs of these patients.
  
  
  CONCLUSIONS
  Our data suggests that the age and gender of THR patients have little impact on their bone marrow osteogenic potential. The significance of the number of the Stro-1(+) with BMPRs expression on the implant fixation and osseointegration warrants further investigation.
authors: Department of Orthopaedic, Renji Hospital, Shanghai JiaoTong University School of Medicine, 1630 DongFang Road, Shanghai 200127, China.
authors: Yi
authors: Y
authors: Shen
authors: Weili
authors: W
authors: Wang
authors: Xiaomiao
authors: X
authors: Li
authors: Zude
authors: Z
authors: Liu
authors: David C
authors: DC
authors: Markel
authors: Weiping
authors: W
authors: Ren
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000032:analysis*; D019644:Arthroplasty, Replacement, Hip; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism*; D052004:Bone Morphogenetic Protein Receptors / Q000378:metabolism*; D002452:Cell Count; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D000954:Antigens, Surface; C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; C525591:STRO-1 antigen, human; D052004:Bone Morphogenetic Protein Receptors