title: Acute toxicity of high dosage carboxymethyl chitosan and its effect on the blood parameters in rats.
abstract: This experiment was aimed to study whether Carboxymethyl chitosan has acute toxicity and effects on the blood parameters of rats, which were treated with high dosage carboxymethyl chitosan (1350 mg/kg) through a laparotomy. Acute toxicity was first studied and then kinds of blood parameters were detected at different time points after the laparotomy, which contain coagulant parameters (thrombin time, prothrombin time, activated partial thromboplatin time and fibrinogen), anticoagulant parameter (antithrombin III), fibrinolytic parameters (plasminogen and fibrin degradation product) and hemorheology parameters (blood viscosity). Results showed that no acute toxicity was detected and no significant effects were found on the parameters of coagulation, anticoagulation, fibrinolysis or hemorheology of rats after the laparotomy, which indicated that carboxymethyl chitosan has no significant toxicity on the blood system of rats after being absorbed in the abdominal cavity and degraded gradually in the blood. And this study has provided experimental basis for carboxymethyl chitosan to be applied in the field of biomedical materials.
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, People's Republic of China.
authors: Zhao
authors: Z
authors: Yang
authors: Baoqin
authors: B
authors: Han
authors: Dawei
authors: D
authors: Fu
authors: Wanshun
authors: W
authors: Liu
mesh_terms: D000042:Absorption; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects*; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D003029:Coagulants / Q000737:chemistry; D005342:Fibrinolysis; D018056:Hemorheology; D007813:Laparotomy / Q000379:methods; D008297:Male; D011134:Polysaccharides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D003029:Coagulants; D011134:Polysaccharides; C514968:carboxymethyl-chitosan; D048271:Chitosan