title: Impaired exercise performance and muscle Na(+),K(+)-pump activity in renal transplantation and haemodialysis patients.
abstract: BACKGROUND
  We examined whether abnormal skeletal muscle Na(+),K(+)-pumps underlie impaired exercise performance in haemodialysis patients (HDP) and whether these are improved in renal transplant recipients (RTx).
  
  
  METHODS
  Peak oxygen consumption ( O(2peak)) and plasma [K(+)] were measured during incremental exercise in 9RTx, 10 HDP and 10 healthy controls (CON). Quadriceps peak torque (PT), fatigability (decline in strength during thirty contractions), thigh muscle cross-sectional area (TMCSA) and vastus lateralis Na(+),K(+)-pump maximal activity, content and isoform (α(1)-α(3), β(1)-β(3)) abundance were measured.
  
  
  RESULTS
  O(2peak) was 32 and 35% lower in RTx and HDP than CON, respectively (P < 0.05). PT was less in RTx and HDP than CON (P < 0.05) but did not differ when expressed relative to TMCSA. Fatigability was ∼1.6-fold higher in RTx (24 ± 11%) and HDP (25 ± 4%) than CON (15 ± 5%, P < 0.05). Na(+),K(+)-pump activity was 28 and 31% lower in RTx and HDP, respectively than CON (P < 0.02), whereas content and isoform abundance did not differ. Pooled (n = 28)  O(2peak) correlated with Na(+),K(+)-pump activity (r = 0.45, P = 0.02).
  
  
  CONCLUSIONS
  O(2peak) and muscle Na(+),K(+)-pump activity were depressed and muscle fatigability increased in HDP, with no difference observed in RTx. These findings are consistent with the possibility that impaired exercise performance in HDP and RTx may be partially due to depressed muscle Na(+),K(+)-pump activity and relative TMCSA.
authors: Muscle, Ions and Exercise Group, Institute of Sport, Exercise and Active Living (ISEAL), Victoria University, Melbourne, Australia.
authors: Aaron C
authors: AC
authors: Petersen
authors: Murray J
authors: MJ
authors: Leikis
authors: Lawrence P
authors: LP
authors: McMahon
authors: Annette B
authors: AB
authors: Kent
authors: Kate T
authors: KT
authors: Murphy
authors: Xiaofei
authors: X
authors: Gong
authors: Michael J
authors: MJ
authors: McKenna
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000502:physiology; D007674:Kidney Diseases / Q000503:physiopathology* / Q000628:therapy*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D018763:Muscle Fatigue / Q000502:physiology; D018482:Muscle, Skeletal / Q000503:physiopathology*; D010101:Oxygen Consumption / Q000502:physiology; D011188:Potassium / Q000097:blood; D006435:Renal Dialysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*
chemical_list: D007527:Isoenzymes; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium