title: Schisandrin B, attenuates cisplatin-induced oxidative stress, genotoxicity and neurotoxicity through modulating NF-κB pathway in mice.
abstract: This study aimed to investigate the potential beneficial effect of an antioxidant lignan, Schisandrin B (Sch B), against cisplatin (cDDP) induced oxidative stress mediated geno- and neuro-toxicities. A dose of 10 mg/kg cDDP induced considerable genotoxicity in mice, and Sch B treatment attenuated the cDDP-induced DNA damage as assessed by the comet assay in the brain. The frequency of micro-nucleated erythrocyte production in bone marrow was also significantly reduced by Sch B treatment in cDDP-treated mice. In neurobehavioral studies, Sch B significantly prevented the memory deficits induced by cDDP, and had an anxiolytic effect in the elevated plus maze task. Sch B treatment significantly attenuated lipid peroxidation, acetylcholinesterase activity and nitrite levels induced by cDDP. Furthermore, Sch B effectively inhibited NF-κB and p53 activation, and cleaved caspase-3 expression in cDDP-treated mice. Hence, Sch B with potent antioxidant and neuro-protective property with no mutagenic activity would be beneficial complementary food factor against cDDP induced oxidative stress.
authors: Department of Functional and Analytical Food Sciences, Niigata University of Pharmacy & Applied Life Sciences (NUPALS), Niigata City, Japan.
authors: Vijayasree V
authors: VV
authors: Giridharan
authors: Rajarajan A
authors: RA
authors: Thandavarayan
authors: Hari N
authors: HN
authors: Bhilwade
authors: Kam M
authors: KM
authors: Ko
authors: Kenichi
authors: K
authors: Watanabe
authors: Tetsuya
authors: T
authors: Konishi
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D053148:Caspase 3 / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D034242:Cyclooctanes / Q000494:pharmacology; D004249:DNA Damage; D004347:Drug Interactions; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009048:Motor Skills / Q000187:drug effects; D009152:Mutagenicity Tests; D016328:NF-kappa B / Q000096:biosynthesis / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D034242:Cyclooctanes; D017705:Lignans; D016328:NF-kappa B; D011083:Polycyclic Compounds; D016159:Tumor Suppressor Protein p53; C015499:schizandrin B; D000110:Acetylcholinesterase; D053148:Caspase 3; D002945:Cisplatin