title: Purification and characterization of a xylanase from Bacillus subtilis isolated from the degumming line.
abstract: Xylanases catalyze the hydrolysis of xylan, a major hemicellulose component of cell wall besides cellulose in most plant species. To extract cellulose fibers, it will be invaluable to screen for more effective xylanase-producing microorganisms. In this paper a new strategy for easy screening of xylanase-producing strains from the degumming line was presented. Using this strategy, a weak-acidic, cellulase-free xylanase from Bacillus subtilis has been isolated, purified and characterized. The xylanase showed high specific activity (36,633.4 U/mg), presented stable characteristics and can be separated and purified simply, with molecular weight 23.3 kD, pI 9.63. It reached its optimal activity at pH 5.8 and 60 °C, and retained over 80% of its maximal activity after pre-incubation at temperature 60 °C or pH 4.6 ~ 6.4. Also, a two-step purification procedure based on the combination of ultrafiltration and gel filtration chromatography was introduced and described, achieving 17-fold purification with 68.11% yield.
authors: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
authors: Gang
authors: G
authors: Guo
authors: Zhengchu
authors: Z
authors: Liu
authors: Junfei
authors: J
authors: Xu
authors: Jianping
authors: J
authors: Liu
authors: Xiaoyang
authors: X
authors: Dai
authors: Daping
authors: D
authors: Xie
authors: Keqing
authors: K
authors: Peng
authors: Xiangyuan
authors: X
authors: Feng
authors: Shengwen
authors: S
authors: Duan
authors: Ke
authors: K
authors: Zheng
authors: Lifeng
authors: L
authors: Cheng
authors: Yueguan
authors: Y
authors: Fu
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004783:Environmental Microbiology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods; D014995:Xylosidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D014995:Xylosidases