title: A novel PIKFYVE mutation in fleck corneal dystrophy.
abstract: OBJECTIVE
  To report the findings of the clinical and molecular evaluation in a Greek family with fleck corneal dystrophy (CFD).
  
  
  METHODS
  A 58-year-old woman was seen on routine ophthalmic examination and diagnosed as having CFD. All available family members were examined to evaluate the clinical findings and inheritance of the disease. Twenty members of the family in five generations underwent slit-lamp examination. Eleven were females and nine males, aged from two years to 85 years old. Blood samples were available from four patients with CFD and seven unaffected relatives, and the DNAs were subjected to molecular screening of the phosphoinositide kinase, five finger-containing (PIKFYVE) gene by direct sequencing or denaturing high performance liquid chromatography (DHPLC).
  
  
  RESULTS
  The clinical evaluation revealed six family members (five females and one male) with CFD. In two CFD patients early cataract formation was noticed. All patients affected with the corneal dystrophy were asymptomatic. The molecular analyses demonstrated the existence of a novel c.3060-3063delCCTT (p.P968Vfs23) mutation in PIKFYVE in all CFD patients tested but in none of the six unaffected family members. No molecular screening was performed in the seventh unaffected member as the causative mutation was clearly transmitted from his affected wife to his affected son.
  
  
  CONCLUSIONS
  We report on the clinical and molecular findings of a five generation Greek family with CFD and we conclude that the novel c.3060-3063delCCTT (p.P968Vfs23) mutation in PIKFYVE, which segregated with the disease, was the causative mutation in this family.
authors: Department of Ophthalmology, General Hospital of Nafplio, Greece.
authors: Andreas
authors: A
authors: Kotoulas
authors: Haris
authors: H
authors: Kokotas
authors: Konstantinos
authors: K
authors: Kopsidas
authors: Konstantinos
authors: K
authors: Droutsas
authors: Maria
authors: M
authors: Grigoriadou
authors: Hasret
authors: H
authors: Bajrami
authors: Daniel F
authors: DF
authors: Schorderet
authors: Michael B
authors: MB
authors: Petersen
mesh_terms: D000368:Aged; D001483:Base Sequence; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000378:metabolism* / Q000473:pathology; D003317:Corneal Dystrophies, Hereditary / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D006115:Greece; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010375:Pedigree; D010641:Phenotype; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*
chemical_list: D019869:Phosphatidylinositol 3-Kinases; C492139:PIKFYVE protein, human