title: Profile of inflammatory mediators in gestational diabetes mellitus: phenotype and genotype.
abstract: OBJECTIVE
  Our study aimed to assess in vitro production of IL-10, IL-6, TNF-A, and adiponectin serum levels in pregnant women with and without gestational diabetes mellitus (GDM) and to investigate a possible association between GDM and IL-10-1082 A>G (rs1800896), IL-6-174 G>C (rs1800795), TNF-A-308 G>A (rs1800629), adiponectin +45 T>G (rs2241766), and adiponectin-11377 C>G (rs266729) gene polymorphisms.
  
  
  METHODS
  This case-control study included 79 women with GDM and 169 healthy controls (C) grouped according to pre-pregnancy BMI. IL-10, IL-6, and TNF-A culture supernatant and adiponectin serum levels were assessed by ELISA. DNA genotype was performed by PCR-RFLP.
  
  
  RESULTS
  Adiponectin levels were significantly higher in C than GDM women, even within the same BMI category. Cytokines levels were similar between the groups. There were no associations between GDM and the analyzed gene polymorphisms.
  
  
  CONCLUSIONS
  Women with GDM have significantly lower adiponectin levels in the third trimester, regardless of BMI.
authors: Department of Obstetrics, Sao Paulo Federal University, Sao Paulo, Brazil.
authors: Bárbara Yasmin
authors: BY
authors: Gueuvoghlanian-Silva
authors: Maria Regina
authors: MR
authors: Torloni
authors: Rosiane
authors: R
authors: Mattar
authors: Lilian Silveira
authors: LS
authors: de Oliveira
authors: Flávia Burim
authors: FB
authors: Scomparini
authors: Mary Uchiyama
authors: MU
authors: Nakamura
authors: Silvia
authors: S
authors: Daher
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D015992:Body Mass Index; D001938:Brazil; D016022:Case-Control Studies; D016640:Diabetes, Gestational / Q000097:blood* / Q000235:genetics*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009765:Obesity / Q000150:complications*; D010641:Phenotype; D011247:Pregnancy
chemical_list: D052242:Adiponectin; D018836:Inflammation Mediators