title: Electrochemical sensor for neurotransmitters at physiological pH using a heterocyclic conducting polymer modified electrode.
abstract: We report the simultaneous determination of two neurotransmitters, norepinephrine (NEP) and serotonin (5-HT), at physiological pH using the electropolymerized film of 3-amino-5-mercapto-1,2,4-triazole modified glassy carbon (p-AMTa) electrode. A bare glassy carbon (GC) electrode fails to resolve the voltammetric signals of NEP and 5-HT due to the surface fouling caused by the oxidized products of them. However, the p-AMTa electrode not only separates the voltammetric signals of NEP and 5-HT with a potential difference of 150 mV between NEP and 5-HT but also shows higher oxidation currents for them. The simultaneous determination of NEP and 5-HT was successfully achieved at p-AMTa electrode using differential pulse voltammetry method. The amperometric current response increased linearly with increasing NEP and 5-HT concentration in the range of 1.0 × 10(-8) to 1.0 × 10(-4) M and 1.0 × 10(-8) to 5.0 × 10(-5) M, respectively, and the detection limit was found to be 1.65 × 10(-11) for NEP and 1.32 × 10(-11) M for 5-HT (S/N = 3). The p-AMTa electrode shows better recovery results for spiked NEP and 5-HT in human blood plasma samples.
authors: Department of Chemistry, Gandhigram Rural Institute, Gandhigram, 624 302 Dindigul, Tamilnadu, India.
authors: S Brillians
authors: SB
authors: Revin
authors: S Abraham
authors: SA
authors: John
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000097:blood; D009638:Norepinephrine / Q000032:analysis* / Q000097:blood; D010084:Oxidation-Reduction; D012701:Serotonin / Q000032:analysis* / Q000097:blood; D014230:Triazoles / Q000737:chemistry*
chemical_list: C488155:3-amino-5-mercapto-1,2,4-triazole; D018377:Neurotransmitter Agents; D014230:Triazoles; D012701:Serotonin; D002244:Carbon; D009638:Norepinephrine