title: [Research of chromosome aberration in Chinese hamster lung fibroblast induced by VEGF siRNA].
abstract: OBJECTIVE
  To explore the effects of VEGF siRNA on chromosome aberration in Chinese hamster lung fibroblasts (CHL).
  
  
  METHODS
  The chromosome aberrations were observed after CHL were transfected with VEGF siRNA for 24 hours and 48h.
  
  
  RESULTS
  There were a questionable positive in 100nmol/L VEGF siRNA after VEGF siRNA were transfected for 24h and the chromosome aberration rate was 6 percentages. Both 50nmol/L VEGF siRNA and 100nmol/L VEGF siRNA caused the questionable positive after VEGF siRNAs were transfected for 48h, and the chromosome aberration rates was 6 percentages and 10 percentages separately. There were no the chromosome aberration in 25nmol/L VEGF siRNA. The types of chromosomal aberration induced by VEGF siRNAs included break, Dicentric grain, polyploid, gap, and three trajectories.
  
  
  CONCLUSIONS
  100nmol/L VEGF siRNA might cause CHL chromosome aberration.
authors: Department of Epidemiology, Medical College, Jinan University, Guangzhou 510632, China. jcxphd@163.com
authors: Chunxia
authors: C
authors: Jing
authors: Huan
authors: H
authors: Zhang
authors: Guang
authors: G
authors: Yang
authors: Chipeng
authors: C
authors: Wu
authors: Lin
authors: L
authors: He
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008168:Lung / Q000166:cytology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000633:toxicity*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A