title: Real-time PCR method applied to seafood products for authentication of European sole (Solea solea) and differentiation of common substitute species.
abstract: Judged by quality and taste, the European sole (Solea solea) is considered one of the finest flatfish and is, thus, of considerable commercial value. In the present work, a specific fast real-time PCR was developed for the authentication of S. solea, i.e. to distinguish it from other related species and avoid substitution of this species, either deliberately or unintentionally. The method is based on a species-specific set of primers and MGB Taqman probe which amplifies a 116-bp fragment of the internal transcribed spacer 1 (ITS 1) ribosomal DNA region. This assay combines the high specificity and sensitivity of real-time PCR with the rapidity of the fast mode, allowing the detection of S. solea in a short period of time. The present methodology was validated for application to all types of manufactured products for the presence of S. solea, with successful results. Subsequently, the method was applied to 40 commercial samples to determine whether correct labeling had been employed in the market. It was demonstrated that the assay is a useful tool in monitoring and verifying food labeling regulations.
authors: Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain.
authors: Beatriz
authors: B
authors: Herrero
authors: Fátima C
authors: FC
authors: Lago
authors: Juan M
authors: JM
authors: Vieites
authors: Montserrat
authors: M
authors: Espiñeira
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005399:Fishes; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D017747:Seafood / Q000592:standards*; D013045:Species Specificity
chemical_list: D017931:DNA Primers