title: Pro-inflammatory effects of ochratoxin A on nasal epithelial cells.
abstract: Eosinophilic chronic rhinosinusitis (ECRS) largely consists of allergic fungal sinusitis, non-allergic fungal rhinosinusitis, aspirin-exacerbated ECRS, and superantigen-induced ECRS. The pathophysiology of ECRS is not completely understood, in particular, the role of mycotoxins remains unknown. The aim of this study was to evaluate the effects of one of the most widespread mycotoxin, ochratoxin A (OTA), on the release of pro-inflammatory cytokines such as interleukin-(IL)-6 and the CXC-chemokine IL-8 from nasal epithelial cell cultures (NEC) of subjects with and without ECRS. NEC (ECRS group: n = 16; controls: n = 7) were stimulated with OTA for 24 h. Protein concentrations of IL-6 and IL-8 levels were measured in NEC supernatants by ELISA prior and 24 h after addition of OTA. Baseline levels in the supernatants of NEC were 183.3 pg/ml for IL-6 and 384.6 pg/ml for IL-8. Stimulation with OTA induced a significant increase of IL-6 (p < 0.001) and IL-8 (p < 0.001) in both NEC of controls and ECRS, respectively. There were no significant differences between controls and ECRS. This is the first study evaluating the effects of a mycotoxin on epithelial airway cells. Our data show that the ubiquitous mycotoxin OTA has a strong pro-inflammatory effect on NEC resulting in the release of IL-6 and IL-8. Mycotoxins may promote inflammation in nasal mucosa.
authors: Department of Haematology and Oncology, University of Cologne, Medical Center, Cologne, Germany.
authors: Birgit
authors: B
authors: Cremer
authors: Alexandra
authors: A
authors: Soja
authors: Jan-Alex
authors: JA
authors: Sauer
authors: Michael
authors: M
authors: Damm
mesh_terms: D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D002908:Chronic Disease; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism*; D009183:Mycotoxins; D009297:Nasal Mucosa / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D009793:Ochratoxins / Q000494:pharmacology*; D012220:Rhinitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D012852:Sinusitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
chemical_list: D002273:Carcinogens; D016207:Cytokines; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A