title: A high prevalence of mupirocin and macrolide resistance determinant among Staphylococcus aureus strains isolated from burnt patients.
abstract: Infections due to Staphylococcus aureus have become increasingly common among burn patients. The antibiotic resistance profile of S. aureus isolates and inducible resistance against clindamycin were investigated in this study. The presence of mecA gene, mupA gene and macrolide resistance genes were detected using PCR and multiplex-PCR. The resistance rate to methicillin, erythromycin and mupirocin were 58.5%, 58% and 40%, respectively. The prevalence of constitutive and inducible resistance among macrolide resistant isolates was 75% and 25%, respectively. Ninety five percent of the isolates were positive for one or more erm genes. The most common genes were ermA (75%), ermC (72%) and ermB (69%), respectively. The ermA gene predominated in the strains with the inducible phenotype, while ermC was more common in the isolates with the constitutive phenotype. The msrA gene was only found in one MRSA isolate with the constitutive phenotype. A total of 27 isolates (25%) carried the mupA gene. All the mupirocin resistant isolates and almost all the erythromycin resistant isolates were also resistant against methicillin which may indicate an outbreak of MRSA isolates with high-level mupirocin and erythromycin resistance in the burn unit assessed.
authors: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Shadi
authors: S
authors: Shahsavan
authors: Mohammad
authors: M
authors: Emaneini
authors: Behshad
authors: B
authors: Noorazar Khoshgnab
authors: Babak
authors: B
authors: Khoramian
authors: Parisa
authors: P
authors: Asadollahi
authors: Marzieh
authors: M
authors: Aligholi
authors: Fereshteh
authors: F
authors: Jabalameli
authors: Mohammad Amin
authors: MA
authors: Eslampour
authors: Morovat
authors: M
authors: Taherikalani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002056:Burns / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D007492:Iran / Q000453:epidemiology; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D016712:Mupirocin / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013207:Staphylococcal Skin Infections / Q000453:epidemiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D016712:Mupirocin