title: Antibody-based protein profiling of the human chromosome 21.
abstract: The Human Proteome Project has been proposed to create a knowledge-based resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level, and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution, and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.
authors: Science for Life Laboratory, Royal Institute of Technology, SE-17121 Solna, Sweden. mathias.uhlen@scilifelab.se
authors: Mathias
authors: M
authors: Uhlén
authors: Per
authors: P
authors: Oksvold
authors: Cajsa
authors: C
authors: Älgenäs
authors: Carl
authors: C
authors: Hamsten
authors: Linn
authors: L
authors: Fagerberg
authors: Daniel
authors: D
authors: Klevebring
authors: Emma
authors: E
authors: Lundberg
authors: Jacob
authors: J
authors: Odeberg
authors: Fredrik
authors: F
authors: Pontén
authors: Tadashi
authors: T
authors: Kondo
authors: Åsa
authors: Å
authors: Sivertsson
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics* / Q000378:metabolism*; D016208:Databases, Factual; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000276:immunology*; D040901:Proteomics; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D012984:Software; D013347:Subcellular Fractions
chemical_list: D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; D020543:Proteome