title: [Activity evaluation of components and preparation of effective components group of xiaoxuming decoction for anti-cerebral ischemic].
abstract: OBJECTIVE
  On basis of preliminary studies, to prepare the effective components group of Xiaoxuming decoction for anti-ischemic by combining chemical analysis with pharmacological activity screening.
  
  
  METHODS
  Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay; TBA method was used to determine mitochondrial lipid peroxidation.
  
  
  RESULTS
  Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest.
  
  
  CONCLUSIONS
  40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China.
authors: Yuehua
authors: Y
authors: Wang
authors: Hailin
authors: H
authors: Qin
authors: Xiaoli
authors: X
authors: He
authors: Guanhua
authors: G
authors: Du
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D016166:Free Radical Scavengers / Q000494:pharmacology* / Q000627:therapeutic use; D018698:Glutamic Acid / Q000009:adverse effects; D006861:Hydrogen Peroxide / Q000009:adverse effects; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D018698:Glutamic Acid; D006861:Hydrogen Peroxide