title: Isolation and characterization of a murine P388 leukemia line resistant to clofarabine.
abstract: A murine P388 leukemia line fully resistant to clofarabine was obtained after only two courses of intraperitoneal treatment (three times a day for nine consecutive days). The resistance was stable for at least 13 weeks without treatment. The subline was as sensitive to 5-fluorouracil, methotrexate, cyclophosphamide, cisplatin, melphalan, BCNU, doxorubicin, etoposide, irinotecan, vincristine, and docetaxel as was the parental P388/0 line but was cross-resistant to five antimetabolites [palmO-ara-C, 4'-thio-ara-C, fludarabine phosphate, cladribine, and gemcitabine-all of which require deoxycytidine kinase for activation] and paclitaxel. The subline had less than 1% of the deoxycytidine kinase activity in comparison to P388/0.
authors: Cancer Therapeutics and Immunology Department, Southern Research Institute, P. O. Box 55305, Birmingham, AL 35255-5305, USA. waud@southernresearch.org
authors: William R
authors: WR
authors: Waud
authors: Karen S
authors: KS
authors: Gilbert
authors: William B
authors: WB
authors: Parker
authors: John A
authors: JA
authors: Secrist
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001087:Arabinonucleosides / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000201:enzymology; D002469:Cell Separation; D000077866:Clofarabine; D003842:Deoxycytidine Kinase / Q000378:metabolism; D019008:Drug Resistance, Neoplasm; D007941:Leukemia P388 / Q000188:drug therapy* / Q000201:enzymology; D051379:Mice
chemical_list: D000227:Adenine Nucleotides; D000970:Antineoplastic Agents; D001087:Arabinonucleosides; D000077866:Clofarabine; D003842:Deoxycytidine Kinase