title: Scanning near-field IR microscopy of proteins in lipid bilayers.
abstract: We use infrared near-field microscopy to chemically map the morphology of biological matrices. The investigated sample is built up from surface-tethered membrane proteins (cytochrome c oxidase) reconstituted in a lipid bilayer. We have carried out infrared near-field measurements in the frequency range between 1600 and 1800 cm(-1). By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer with a lateral resolution of 80 nm × 80 nm, we were able to probe locally the chemical signature of this membrane and to provide a local map of its surface morphology.
authors: Department of Physical Chemistry II, Ruhr-University Bochum, 44801 Bochum, Germany.
authors: Fouad
authors: F
authors: Ballout
authors: Henning
authors: H
authors: Krassen
authors: Ilona
authors: I
authors: Kopf
authors: Kenichi
authors: K
authors: Ataka
authors: Erik
authors: E
authors: Bründermann
authors: Joachim
authors: J
authors: Heberle
authors: Martina
authors: M
authors: Havenith
mesh_terms: D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D019265:Spectroscopy, Near-Infrared
chemical_list: D008051:Lipid Bilayers; D003576:Electron Transport Complex IV