title: Bacillus subtilis subspecies virginiana, a new subspecies of antitermitic compound-producing endophytic bacteria isolated from Juniperus virginiana.
abstract: Termites are worldwide pests causing considerable damage to agriculture, forestry and buildings. Although physical and chemical methods have been tried to eliminate termite populations, they have the limitations such as low effectiveness, high-toxicity residue, environmentally harmful and high cost. Therefore, it has attracted much attention to develop highly effective, low-toxic, long residual period, environmentally friendly and low-cost termiticidals. Here, we report the characterization and antitermitic activities of a new antitermitic compound-producing endophytic bacterium HUB-I-47 isolated from eastern red-cedar, Juniperus virginiana L. The morphological, physiochemical characteristics of strain HUB-I-47 and its 16S rDNA sequences, and the antitermitic compound were analyzed by gas chromatography-mass spectrometry were studied. We found that the morphology of HUB-I-47 was very similar to that of Bacillus subtilis but presented some differences in shape and cell size. Growth evaluation showed that the lowest, highest, and optimum growth temperatures of HUB-I-47 were 12, 47, and 31 degrees C, respectively, which were different from those of reference strains. The 16S rDNA sequence analysis revealed a high similarity of 99% to those of B. subtilis. Based on these analyses, we named strain HUB-I-47 as B. subtilis subsp. virginiana D. P. Zhou, K. Zhao, J. Liu et W. X. Ping, subsp. nov. This is the first report on the analysis of antitermitic compounds from endophytic bacteria. Our study identified a new resource of antitermitic compounds through endophytic bacteria fermentation.
authors: Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, 74 Xuefu Rd., Harbin 150080, People's Republic of China.
authors: Kai
authors: K
authors: Zhao
authors: Jun
authors: J
authors: Liu
authors: Zhugang
authors: Z
authors: Li
authors: Zhiwei
authors: Z
authors: Chang
authors: Pengfei
authors: P
authors: Shi
authors: Wenxiang
authors: W
authors: Ping
authors: Dongpo
authors: D
authors: Zhou
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D002681:China; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D020049:Isoptera / Q000187:drug effects / Q000382:microbiology*; D018653:Juniperus / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S