title: The antinociceptive efficacy of HWTX-I epidurally administered in rheumatoid arthritis rats.
abstract: Rheumatoid arthritis (RA) is one of the inflammatory kinds of arthritis in the clinical situation, and cytosolic Ca2+ overload has been proposed as one of the primary factors for many inflammatory cells activation, which lead to relative enzymes and inflammatory factors release. It is therefore accepted that Ca2+ channel blockers can protect joint injury from inflammation. In the present study we investigated the possible molecular mechanism of the antinociceptive efficacy of HWTX-I, a spider peptide toxin blocking Ca2+ channels, on the rat rheumatoid arthritis model. Our study demonstrates that HWTX-I can relieve pain in the inflammatory joints and eliminate arthrocele to some degree. Moreover, HWTX-I can also decrease the concentration of tumour necrosis factor α (TNF-α) and increase the concentration of interleukin 4(IL-4) and interleukin 10(IL-10) in rat's serum. HWTX-I can also decrease the mRNA expression level of related factors of TNF-α, interleukin 1β (IL-1β) and interleukin 6(IL-6) in inflammatory pathways in rheumatoid arthritis. Therefore, the present results show that the epidural administration of HWTX-I is effective in antinociception in the rat model of rheumatoid arthritis, which may act through its inhibition on certain inflammatory pathways.
authors: Hunan Normal University, College of Physical Education, Changsha, China.
authors: Z
authors: Z
authors: Wen Tao
authors: T
authors: T
authors: Gu Yang
authors: R
authors: R
authors: Ying
authors: W
authors: W
authors: Mao Cai
authors: L
authors: L
authors: Lin
authors: L
authors: L
authors: Chi Miao
authors: H
authors: H
authors: Peng
authors: C
authors: C
authors: Joa Qin
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D007268:Injections, Epidural; D008297:Male; D009498:Neurotoxins / Q000008:administration & dosage / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D030162:Reptilian Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D013111:Spider Venoms / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D016207:Cytokines; D009498:Neurotoxins; D030162:Reptilian Proteins; D013111:Spider Venoms; C083624:huwentoxin I; D002118:Calcium