title: Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae.
abstract: Human parvovirus 4 (PARV4) is an emerging human virus, and little is known about the molecular aspects of PARV4 apart from its incomplete genome sequence, which lacks information of the termini. We analyzed the gene expression profile of PARV4 using a nearly full-length HPV4 genome in a replication competent system in 293 cells. We found that PARV4 utilizes two promoters to transcribe non-structural protein- and structural protein-encoding mRNAs, respectively, which were polyadenylated at the right end of the genome. Three major proteins, including the large non-structural protein NS1a, whose mRNA is spliced, and capsid proteins VP1 and VP2, were detected. Additional functional analysis of the NS1a revealed its capability to induce cell cycle arrest at G2/M phase in ex vivo-generated human hematopoietic stem cells. Taken together, our characterization of the molecular features of PARV4 suggests that PARV4 represents a new genus in the family Parvoviridae.
authors: Department of Infectious Diseases, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors: Sai
authors: S
authors: Lou
authors: Baoyan
authors: B
authors: Xu
authors: Qinfeng
authors: Q
authors: Huang
authors: Ning
authors: N
authors: Zhi
authors: Fang
authors: F
authors: Cheng
authors: Susan
authors: S
authors: Wong
authors: Kevin
authors: K
authors: Brown
authors: Eric
authors: E
authors: Delwart
authors: Zhengwen
authors: Z
authors: Liu
authors: Jianming
authors: J
authors: Qiu
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D016679:Genome, Viral; D057809:HEK293 Cells; D006412:Hematopoietic Stem Cells / Q000821:virology; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology; D017992:Parvovirus / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D012326:RNA Splicing; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D017361:Viral Nonstructural Proteins