title: Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes.
abstract: Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 ± 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and strongly inhibited virus infection in an in vitro virus neutralization assay. In order to make tomatoes expressing hIgA_2A1 easily distinguishable from wild-type tomatoes, lines expressing hIgA_2A1 transgenes were sexually crossed with a transgenic tomato line expressing the genes encoding Antirrhinum majus Rosea1 and Delila transcription factors, which confer purple colour to the fruit. Consequently, transgenically labelled purple tomato fruits expressing hIgA_2A1 have been developed. The resulting purple-coloured extracts from these fruits contain high levels of recombinant anti-rotavirus neutralizing human IgA in combination with increased amounts of health-promoting anthocyanins.
authors: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Valencia, Spain.
authors: Paloma
authors: P
authors: Juárez
authors: Silvia
authors: S
authors: Presa
authors: Joaquín
authors: J
authors: Espí
authors: Benito
authors: B
authors: Pineda
authors: María T
authors: MT
authors: Antón
authors: Vicente
authors: V
authors: Moreno
authors: Javier
authors: J
authors: Buesa
authors: Antonio
authors: A
authors: Granell
authors: Diego
authors: D
authors: Orzaez
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000872:Anthocyanins / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D032161:Antirrhinum / Q000235:genetics; D015153:Blotting, Western; D003433:Crosses, Genetic; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology; D018551:Solanum lycopersicum / Q000235:genetics / Q000276:immunology* / Q000821:virology; D009500:Neutralization Tests; D010858:Pigmentation; D010936:Plant Extracts / Q000276:immunology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology* / Q000821:virology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012401:Rotavirus / Q000276:immunology*; D013194:Staining and Labeling; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019076:Transgenes; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000872:Anthocyanins; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D010936:Plant Extracts; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus