title: Acid-sensing ion channel 3 mediates peripheral anti-hyperalgesia effects of acupuncture in mice inflammatory pain.
abstract: BACKGROUND
  Peripheral tissue inflammation initiates hyperalgesia accompanied by tissue acidosis, nociceptor activation, and inflammation mediators. Recent studies have suggested a significantly increased expression of acid-sensing ion channel 3 (ASIC3) in both carrageenan- and complete Freund's adjuvant (CFA)-induced inflammation. This study tested the hypothesis that acupuncture is curative for mechanical hyperalgesia induced by peripheral inflammation.
  
  
  METHODS
  Here we used mechanical stimuli to assess behavioral responses in paw and muscle inflammation induced by carrageenan or CFA. We also used immunohistochemistry staining and western blot methodology to evaluate the expression of ASIC3 in dorsal root ganglion (DRG) neurons.
  
  
  RESULTS
  In comparison with the control, the inflammation group showed significant mechanical hyperalgesia with both intraplantar carrageenan and CFA-induced inflammation. Interestingly, both carrageenan- and CFA-induced hyperalgesia were accompanied by ASIC3 up-regulation in DRG neurons. Furthermore, electroacupuncture (EA) at the ST36 rescued mechanical hyperalgesia through down-regulation of ASIC3 overexpression in both carrageenan- and CFA-induced inflammation.
  
  
  CONCLUSIONS
  In addition, electrical stimulation at the ST36 acupoint can relieve mechanical hyperalgesia by attenuating ASIC3 overexpression.
authors: College of Agriculture and Natural Resources, Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Wei-Hsin
authors: WH
authors: Chen
authors: Ching-Liang
authors: CL
authors: Hsieh
authors: Chun-Ping
authors: CP
authors: Huang
authors: Tzu-Jou
authors: TJ
authors: Lin
authors: Jason Tc
authors: JT
authors: Tzen
authors: Tin-Yun
authors: TY
authors: Ho
authors: Yi-Wen
authors: YW
authors: Lin
mesh_terms: D062565:Acid Sensing Ion Channels; D015670:Acupuncture Therapy; D000818:Animals; D002351:Carrageenan; D005620:Freund's Adjuvant; D005727:Ganglia, Spinal / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000378:metabolism; D010146:Pain / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D059408:Pain Management; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C508378:ASIC3 protein, mouse; D062565:Acid Sensing Ion Channels; D015222:Sodium Channels; D002351:Carrageenan; D005620:Freund's Adjuvant