title: Induction of arginine vasopressin-enhanced green fluorescent protein expression in the locus coeruleus following kainic acid-induced seizures in rats.
abstract: Seizure causes autonomic, neuroendocrine and stress responses. We examined the effects of kainic acid (KA)-induced seizures on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) in the locus coeruleus (LC), an area known to contain noradrenergic cells, in AVP-eGFP transgenic male and female rats, with the rationale to identify stressors which induce AVP synthesis in the LC. Subcutaneous (s.c.) administration of KA caused a progressive development of seizure behavior within 24 h. AVP-eGFP fluorescence in the LC was detected 6, 24, and 48 h and 1 week after administration of KA (12 mg/kg). From a nearly undetectable level, it reached a maximum at 48 h after s.c. administration of KA and returned to the basal levels after 2 weeks. AVP-eGFP fluorescence in the LC after s.c. administration of KA was significantly reduced by the pretreatment with MK-801 (nonselective N-methyl-D-aspartate (NMDA) receptor antagonist). In the KA-administered rats, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localized with TH-immuno-reactivity in the LC. These results suggest that the synthesis of AVP-eGFP is potentially up-regulated in noradrenergic neurons in the LC after KA-induced seizures through the activation of NMDA receptors.
authors: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, and Kokura Rehabilitation Hospital, Yahatanishi-ku, Kitakyushu, Japan.
authors: Motoko
authors: M
authors: Ohno
authors: Hiroaki
authors: H
authors: Fujihara
authors: Masaru
authors: M
authors: Iwanaga
authors: Miwako
authors: M
authors: Todoroki
authors: Akiko
authors: A
authors: Katoh
authors: Toyoaki
authors: T
authors: Ohbuchi
authors: Toru
authors: T
authors: Ishikura
authors: Akinori
authors: A
authors: Hamamura
authors: Kenji
authors: K
authors: Hachisuka
authors: Yoichi
authors: Y
authors: Ueta
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001127:Arginine Vasopressin / Q000235:genetics*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D007608:Kainic Acid; D008125:Locus Coeruleus / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D015854:Up-Regulation
chemical_list: D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D001127:Arginine Vasopressin; D049452:Green Fluorescent Proteins; D016291:Dizocilpine Maleate; D014446:Tyrosine 3-Monooxygenase; D007608:Kainic Acid