title: mTOR-rictor is the Ser473 kinase for AKT1 in mouse one-cell stage embryos.
abstract: Mammalian target of rapamycin (mTOR) controls cell growth and proliferation via the raptor-mTOR (TORC1) and rictor-mTOR (TORC2) protein complexes. The mTORC2 containing mTOR and rictor is thought to be rapamycin insensitive and it is recently shown that both rictor and mTORC2 are essential for the development of both embryonic and extra embryonic tissues. To explore rictor function in the early development of mouse embryos, we disrupted the expression of rictor, a specific component of mTORC2, in mouse fertilized eggs by using rictor shRNA. Our results showed that one-cell stage eggs that were lack of rictor could not enter into the two-cell stage normally. Recent biochemical studies suggests that TORC2 is the elusive PDK2 (3'-phosphoinositide-dependent kinase 2) for AKT/PKB Ser473 phosphorylation, which is deemed necessary for AKT function, so we microinjected AKT-S473A into mouse fertilized eggs to investigate whether AKT-S473A is downstream effector of mTOR.rictor to regulate the mitotic division. Our findings revealed that the rictor induced phosphorylation of AKT in Ser473 is required for TORC2 function in early development of mouse embryos.
authors: Department of Urology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.
authors: Zhe
authors: Z
authors: Zhang
authors: Guojun
authors: G
authors: Zhang
authors: Xiaoyan
authors: X
authors: Xu
authors: Wenhui
authors: W
authors: Su
authors: Bingzhi
authors: B
authors: Yu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D046149:Embryo Culture Techniques; D004789:Enzyme Activation; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D008297:Male; D051379:Mice; D011485:Protein Binding; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; D000076223:Regulatory-Associated Protein of mTOR; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D015053:Zygote / Q000201:enzymology* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C504923:Crtc2 protein, mouse; D000076226:Rapamycin-Insensitive Companion of mTOR Protein; D000076223:Regulatory-Associated Protein of mTOR; C540162:Rptor protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C525637:rictor protein, mouse; C514862:Akt1 protein, mouse; D051057:Proto-Oncogene Proteins c-akt