title: RMRP is a non-coding RNA essential for early murine development.
abstract: RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development.
authors: Department of Medical Oncology, Dana-Farber Cancer Institute, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.
authors: Joseph
authors: J
authors: Rosenbluh
authors: Deepak
authors: D
authors: Nijhawan
authors: Zhao
authors: Z
authors: Chen
authors: Kwok-Kin
authors: KK
authors: Wong
authors: Kenkichi
authors: K
authors: Masutomi
authors: William C
authors: WC
authors: Hahn
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D005804:Genes, Lethal; D051379:Mice; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering; D022661:RNA, Untranslated / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D034741:RNA, Small Interfering; D022661:RNA, Untranslated