title: Antigenic specificity of monoclonal antibodies to beef myoglobin determined by cross-reactivity studies against myoglobins from domestic species.
abstract: Six rat monoclonal antibodies to beef myoglobin were studied to pinpoint the antigenic determinants they recognize. Their ability to bind myoglobin from beef, sheep, goat, horse, pig and chicken was compared in a competitive ELISA using biotinylated beef myoglobin. Correlation of sequence differences with relative binding allowed us to identify critical antigenic residues recognized by these antibodies. Each domain included residues previously considered not to be directly involved in the antigenic structure of myoglobin. Moreover, a possible orientation of myoglobin when adsorbed onto plastic surfaces was defined. These antibodies should be valuable tools for analysing conformational changes of the protein occurring during chemical or physical treatments.
authors: INRA, Meat Research Station, Immunochemistry Unit, Theix, 63122, Saint-Genès Champanelle, France.
authors: D
authors: D
authors: Levieux
authors: A
authors: A
authors: Levieux