title: Technical note: A simplified procedure for myofibril hydrophobicity determination.
abstract: A simple and reliable method for the determination of surface hydrophobicity of nonsolubilized myofibrils (from pig M. longissimus dorsi) was developed and validated. This method is based on the interaction of the hydrophobic chromophore bromophenol blue (BPB) with myofibrillar proteins and the separation of free and bound BPB by centrifugation. The titration of bound BPB is performed by absorption spectroscopy, and the amount of bound BPB is considered as an index of protein hydrophobicity. Heating, which is known to increase protein hydrophobicity, was performed in order to validate this method. Fixation of BPB to myofibrils increased with heating time and temperature, strongly suggesting that it may be closely related to protein hydrophobicity.
authors: Qualité des Produits Animaux, INRA, Centre de Theix, 63122 St Genès Champanelle, France.
authors: Ilham
authors: I
authors: Chelh
authors: Philippe
authors: P
authors: Gatellier
authors: Véronique
authors: V
authors: Santé-Lhoutellier