title: Natural recovery from antiglomerular basement membrane glomerulonephritis is associated with glomeruli-infiltrating CD8α+CD11c+MHC class II+ cells.
abstract: OBJECTIVE
  In an antiglomerular basement membrane glomerulonephritis (GN) model, GN-resistant Lewis (LEW) rats naturally recover from early glomerular inflammation (days 21-23). We have previously identified a glomeruli-infiltrating CD8α(+)CD11(high)MHC II(+) cell (GIL CD8α(+) cell) in GN-prone Wistar Kyoto (WKY) rats, which terminates glomerular inflammation through inducing T cell apoptosis prior to glomerular fibrosis at days 35-40. We investigated if GIL CD8α(+) cells were also associated with the recovery in LEW rats.
  
  
  METHODS
  GIL CD8α(+) cells in LEW rats were characterized; their infiltration was observed in connection with T cell apoptosis in glomeruli.
  
  
  RESULTS
  An influx of GIL CD8α(+) cells into inflamed glomeruli was confirmed in the immunized LEW rats at days 17-22, which was much earlier than days 28-35 in WKY rats. Notably, LEW rats had a GIL CD8α(+)CD11(high) subpopulation after day 17, while WKY rats lacked this population until after day 30. Analyses further revealed a large number of clustered apoptotic CD4(+) or CD3(+) T cells in the glomeruli during recovery (day 23) in LEW rats, as compared to day 35 (transition to fibrosis) in WKY rats. Thus, infiltration of GIL CD8α(+) cells coincided with decline of glomerular inflammation and T cell apoptosis during recovery in LEW rats. Isolated GIL CD8α(+) cells were able to infiltrate glomeruli in both WKY and LEW rats at day 20.
  
  
  CONCLUSIONS
  Our data revealed a strong association between GIL CD8a+ cells and recovery from early glomerular inflammation. It raises a possibility of involvement of GIL CD8a+ cells in the recovery.
authors: Dental Branch, Department of Diagnostic Sciences, University of Texas Health Science Center at Houston, USA.
authors: Cindy
authors: C
authors: Zhou
authors: Julie
authors: J
authors: Robertson
authors: Jean
authors: J
authors: Wu
authors: Todd
authors: T
authors: Bartkowiak
authors: Kiana
authors: K
authors: Parker
authors: John
authors: J
authors: McMahon
authors: Ya-Huan
authors: YH
authors: Lou
mesh_terms: D000818:Animals; D017209:Apoptosis; D001323:Autoantibodies / Q000276:immunology*; D039521:CD11c Antigen / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005921:Glomerulonephritis / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007249:Inflammation; D007678:Kidney Glomerulus / Q000276:immunology* / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew; D011921:Rats, Inbred WKY; D013601:T-Lymphocytes / Q000166:cytology; D013997:Time Factors
chemical_list: D001323:Autoantibodies; D039521:CD11c Antigen; D016827:CD8 Antigens; C431698:CD8alpha antigen; D000949:Histocompatibility Antigens Class II; C051951:antiglomerular basement membrane antibody