title: Effects of different activation regimens on pronuclear formation and developmental competence of in vitro-matured porcine oocytes after intracytoplasmic sperm injection.
abstract: To improve the activation protocol for in vitro-maturated porcine oocytes after intracytoplasmic sperm injection (ICSI), we examined the combined effect of U0126, a specific inhibitor of mitogen-activated protein kinase kinases, and an electrical pulse on pronuclear formation and developmental competence. Two approaches were tested: (i) 6-h treatment of ICSI oocytes with U0126 applied at different intervals (0, 2, 3 or 4 h) after the electrical pulse and (ii) treatment of ICSI oocytes with U0126 applied 4 h after the electrical pulse over an additional 4, 6 or 8 h. Another protein kinase inhibitor, 6-dimethylaminopurine, was used as a chemical activator in control experiments. The highest rates of diploid embryo formation, normal fertilization and blastocyst formation were observed after 6 h of exposure to U0126 starting 4 h after the electrical pulse. Therefore, U0126 can be used as an activating agent for porcine oocytes fertilized by ICSI.
authors: Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Sciences and Technology, China Agricultural University, Beijing, China.
authors: W-M
authors: WM
authors: Cheng
authors: Z-H
authors: ZH
authors: Wu
authors: X
authors: X
authors: Zhang
authors: Y-B
authors: YB
authors: Zhu
authors: Y-W
authors: YW
authors: Pang
authors: M
authors: M
authors: Guo
authors: D
authors: D
authors: Wang
authors: J-H
authors: JH
authors: Tian
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002070:Butadienes / Q000494:pharmacology; D004558:Electric Stimulation; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D009570:Nitriles / Q000494:pharmacology; D009865:Oocytes / Q000254:growth & development* / Q000648:ultrastructure*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary*; D013552:Swine
chemical_list: D002070:Butadienes; D009570:Nitriles; D047428:Protein Kinase Inhibitors; C113580:U 0126; C001020:N(6),N(6)-dimethyladenine; D020929:Mitogen-Activated Protein Kinase Kinases; D000225:Adenine