title: Promoter of TFPI-2 is hypermethylated in Chinese pediatric acute myeloid leukemia.
abstract: Human tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a metastasis-associated gene in many types of tumors. In this study, we investigated whether TFPI-2 was inactivated epigenetically in pediatric acute myeloid leukemia (AML). Methylation status was investigated by methylation-specific polymerase chain reaction and bisulfate genomic sequencing. TFPI-2 was aberrantly methylated in 50% (3/6) of AML cell lines. Aberrant methylation of TFPI-2 promoter was detected in 71.6% (48/67) of the Chinese pediatric AML patients. TFPI-2 transcript was significantly lower in AML group compared with controls (3.44 vs. 32.8, P<0.001). Patients with methylated TFPI-2 gene had significantly lower TFPI-2 transcript than those patients without methylated TFPI-2 (P=0.04). Promoter hypermethylation of TFPI-2 is frequent and specific event in pediatric AML.
authors: Department of Hematology and Oncology, Children’s Hospital of Soochow University, Suzhou, China.
authors: Pan
authors: P
authors: Jian
authors: Wu Shui
authors: WS
authors: Yan
authors: Sun Li
authors: SL
authors: Chao
authors: Peng
authors: P
authors: Liang
authors: Li
authors: L
authors: Zhen
authors: Qiu Bao
authors: QB
authors: Ling
authors: Li Yan
authors: LY
authors: Hong
authors: Li Yi
authors: LY
authors: Ping
authors: Wang
authors: W
authors: Jian
authors: Jing Mei
authors: JM
authors: Fang
authors: Liu
authors: L
authors: Ling
authors: Wang Xing
authors: WX
authors: Dong
authors: Zhu Xue
authors: ZX
authors: Ming
authors: Ni
authors: N
authors: Jian
mesh_terms: D000293:Adolescent; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D006023:Glycoproteins; D012333:RNA, Messenger; C021240:tissue-factor-pathway inhibitor 2