title: In vitro properties of platelets stored in three different additive solutions.
abstract: BACKGROUND
  New platelet (PLT) additive solutions (PASs) contain compounds that might improve the storage conditions for PLTs. This study compares the in vitro function, including hemostatic properties (clot formation and elasticity), of PLTs in T-Sol, Composol, or SSP+ during storage for 5 days.
  
  
  METHODS
  Fifteen buffy coats were pooled and divided into three parts. PLT concentrates (PCs) with 30% plasma and 70% PAS (T-Sol, Composol, or SSP+) were prepared (n = 10). Swirling, PLT count, blood gases, metabolic variables, PLT activation markers, and coagulation by free oscillation rheometry (FOR) were analyzed on Days 1 and 5.
  
  
  RESULTS
  Swirling was well preserved and pH acceptable (6.4-7.4) during storage for all PASs. Storage of PLTs in T-Sol led to a decrease in PLT count whereas the number of PLTs was unchanged in Composol or SSP+ PCs. PLTs in T-Sol showed higher glucose metabolism than PLTs in Composol or in SSP+. At the end of storage PLTs in T-Sol had higher spontaneous activation and lower ability to respond to an agonist than PLTs in Composol or SSP+. PLTs in all the PASs had a similar ability to promote clot formation and clot elasticity.
  
  
  CONCLUSIONS
  Storage of PLTs in Composol or in SSP+ improved the quality of PCs in terms of better maintained PLT count, lower glucose metabolism, lower spontaneous activation, and improved response to a PLT agonist compared to PLTs in T-Sol. PLTs stored in the various PASs had similar hemostatic properties. These findings make Composol and SSP+ interesting alternatives as PASs.
authors: Division of Transfusion Medicine, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
authors: Nahreen
authors: N
authors: Tynngård
authors: Marie
authors: M
authors: Trinks
authors: Gösta
authors: G
authors: Berlin
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010976:Platelet Count; D012996:Solutions; D014914:Whole Blood Coagulation Time
chemical_list: D012996:Solutions