title: Charge specific protein placement at submicrometer and nanometer scale by direct modification of surface potential by electron beam.
abstract: The understanding and the precise control of protein adsorption is extremely important for the development and optimization of biomaterials. The challenge resides in controlling the different surface properties, such as surface chemistry, roughness, wettability, or surface charge, independently, as modification of one property generally affects the other. We demonstrate the creation of electrically modified patterns on hydroxyapatite by using scanning electron beam to tailor the spatial regulation of protein adsorption via electrostatic interactions without affecting other surface properties of the material. We show that domains, presenting modulated surface potential, can be created to precisely promote or reduce protein adsorption.
authors: Chemical and Environmental Science Department and Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.
authors: Sylvain
authors: S
authors: Robin
authors: Abbasi A
authors: AA
authors: Gandhi
authors: Maros
authors: M
authors: Gregor
authors: Fathima R
authors: FR
authors: Laffir
authors: Tomas
authors: T
authors: Plecenik
authors: Andrej
authors: A
authors: Plecenik
authors: Tewfik
authors: T
authors: Soulimane
authors: Syed A M
authors: SA
authors: Tofail
mesh_terms: D000327:Adsorption; D000818:Animals; D018786:Bone Substitutes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D017886:Durapatite / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D004583:Electrons; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019736:Prostheses and Implants; D055672:Static Electricity; D017155:Wettability; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D017886:Durapatite; C421864:hen egg lysozyme; D009113:Muramidase; D016650:Fluorescein-5-isothiocyanate