title: The evolution of human immunodeficiency virus type-1 (HIV-1) envelope molecular properties and coreceptor use at all stages of infection in an HIV-1 donor-recipient pair.
abstract: To trace the evolutionary patterns underlying evolution of coreceptor use within a host, we studied an HIV-1 transmission pair involving a donor who exclusively harbored CCR5-using (R5) variants throughout his entire disease course and a recipient who developed CXCR4-using variants. Over time, R5 variants in the donor optimized coreceptor use, which was associated with an increased number of potential N-linked glycosylation sites (PNGS) and elevated V3 charge in the viral envelope. Interestingly, R5 variants that were transmitted to the recipient preserved the viral characteristics of this late stage genotype and phenotype. Following a selective sweep, CXCR4-using variants subsequently emerged in the recipient coinciding with a further increase in the number of PNGS and V3 charge in the envelope of R5 viruses. Although described in a single transmission pair, the transmission and subsequent persistence of R5 variants with late stage characteristics demonstrate the potential for coreceptor use adaptation at the population level.
authors: Department of Experimental Immunology, Sanquin Research, Landsteiner Laboratory, part of the Center for Infectious Diseases and Immunity Amsterdam at the Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands.
authors: Diana
authors: D
authors: Edo-Matas
authors: Andrea
authors: A
authors: Rachinger
authors: Laurentia C
authors: LC
authors: Setiawan
authors: Brigitte D
authors: BD
authors: Boeser-Nunnink
authors: Angélique B
authors: AB
authors: van 't Wout
authors: Philippe
authors: P
authors: Lemey
authors: Hanneke
authors: H
authors: Schuitemaker
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D015658:HIV Infections / Q000235:genetics / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D019713:Receptors, CCR5 / Q000235:genetics / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: C527276:CXCR4 protein, human; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D014759:Viral Envelope Proteins