title: Microarray method for the rapid detection of glycosaminoglycan-protein interactions.
abstract: Glycosaminoglycans (GAGs) perform numerous vital functions within the body. As major components of the extracellular matrix, these polysaccharides participate in a diverse array of cell-signaling events. We have developed a simple microarray assay for the evaluation of protein binding to various GAG subclasses. In a single experiment, the binding to all members of the GAG family can be rapidly determined, giving insight into the relative specificity of the interactions and the importance of specific sulfation motifs. The arrays are facile to prepare from commercially available materials.
authors: Division of Chemistry and Chemical Engineering, California Institute of Technology and the Howard Hughes Medical Institute, Pasadena, CA, USA.
authors: Claude J
authors: CJ
authors: Rogers
authors: Linda C
authors: LC
authors: Hsieh-Wilson
mesh_terms: D006025:Glycosaminoglycans / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D006025:Glycosaminoglycans; D011506:Proteins