title: Protective effect of polysaccharide from Hizikia fusiformis against ethanol-induced toxicity.
abstract: Polysaccharide extracted from Hizikia fusiformis (Hf-PS-1) exhibited protective effects against ethanol-induced peptic injury. In in vivo assay, the ethanol group exhibited decrease of total glutathione (GSH) and increase of jun N-terminal kinase (JNK) phosphorylation relative to the control group, whereas levels were significantly increased and decreased, respectively, in the Hf-PS-1 group. Hf-PS-1 reduced ethanol-induced gastric injury. In in vitro assay, ethanol induced IEC-6 cells' death in a dose-dependent manner. Ethanol decreased the phosphorylation of Shc and the binding of Grb2 to Shc, and Hf-PS-1 pretreatment increased them. Ethanol also induced the phosphorylation of JNK and extracellular signal-regulated kinase (ERK), whereas Hf-PS-1 pretreatment decreased JNK activation but not ERK. Co-treatment with JNK inhibitor and ethanol decreased GSH levels, indicating that JNK phosphorylation is a critical factor during ethanol-induced injury. Therefore, Hf-PS-1 may be useful to protect against ethanol-induced gastrointestinal injury.
authors: College of Fisheries Science, Pukyong National University, Busan, Republic of Korea.
authors: Hye-Jung
authors: HJ
authors: Hwang
authors: In-Hye
authors: IH
authors: Kim
authors: Taek-Jeong
authors: TJ
authors: Nam
mesh_terms: D000818:Animals; D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D005756:Gastritis / Q000139:chemically induced* / Q000517:prevention & control; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000166:cytology / Q000187:drug effects* / Q000473:pathology
chemical_list: D011134:Polysaccharides; D000431:Ethanol; D048670:MAP Kinase Kinase 4