title: Sensitive detection and identification of DNA and RNA using a patterned capillary tube.
abstract: We report on a new ultrasensitive and fast technique for the detection and identification of both DNA and RNA with sensitivity of a few molecules. The new method is based on a patterned capillary tube (PCT) in which the internal surface of a glass tube is patterned with rings of different single-stranded DNA probes. A solution containing single-stranded analyte flows through the tube. Upon hybridization of appropriate DNA and RNA from the solution, DNA polymerase and reverse transcriptase (RT) are employed to synthesize the complementary nucleic acids with deoxynucleoside triphosphate (dNTP) labeled with fluorophores. The sample-analyte hybrids are detected by their fluorescence signal. We show that the new method is sensitive, is specific, can detect simultaneously both DNA and RNA from the same sample, and allows detection of analytes in serum.
authors: Department of Chemical Physics, The Weizmann Institute, Rehovot, Israel.
authors: Amos
authors: A
authors: Bardea
authors: Noa
authors: N
authors: Burshtein
authors: Yinon
authors: Y
authors: Rudich
authors: Tomer
authors: T
authors: Salame
authors: Carmit
authors: C
authors: Ziv
authors: Oded
authors: O
authors: Yarden
authors: Ron
authors: R
authors: Naaman
mesh_terms: D004247:DNA / Q000032:analysis* / Q000096:biosynthesis; D015342:DNA Probes / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D015347:RNA Probes / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D015347:RNA Probes; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase