title: Glycoarray by DNA-directed immobilization.
abstract: Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the immobilization process. Herein a protocol for the synthesis of oligonucleotide glycomimetic conjugates is proposed. The resulting molecules are immobilized by hybridization on a DNA microarray (DNA-directed immobilization; DDI). DDI has been proved to be a very efficient and site-selective. This protocol provides detailed procedures for the preparation of fluorescent oligonucleotide trigalactosylmimetic conjugates and for the preparation of carbohydrate microarrays by DDI on glass slides.
authors: Institut des Biomoléules Max Mousseron, UMR 5247, CNRS Université Montpellier 1, Université Montpellier 2, Montpellier, France.
authors: François
authors: F
authors: Morvan
authors: Yann
authors: Y
authors: Chevolot
authors: Jing
authors: J
authors: Zhang
authors: Albert
authors: A
authors: Meyer
authors: Sébastien
authors: S
authors: Vidal
authors: Jean-Pierre
authors: JP
authors: Praly
authors: Jean-Jacques
authors: JJ
authors: Vasseur
authors: Eliane
authors: E
authors: Souteyrand
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*
chemical_list: D002241:Carbohydrates; D004247:DNA