title: Simultaneous determination of flucloxacillin and ampicillin in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and subsequent application to a clinical study in healthy Chinese volunteers.
abstract: A novel method has been developed for the simultaneous determination of flucloxacillin and ampicillin in human plasma by ultra performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The plasma was treated by single step of protein precipitation (PPT) with acetonitrile. The chromatographic separation was performed with a mobile phase consisting of 10mM ammonium formate and acetonitrile (68:32, v/v). The analyses were carried out by multiple reaction monitoring (MRM) using the precursor-to-product combinations of m/z 454.1→160.3 (flucloxacillin), m/z 350.1→106.4 (ampicillin) and m/z 436.1→277.3 (IS). Validation results indicated that the lower limit of quantification (LLOQ) were both 0.2 μg/mL and both assay exhibited a linear range of 0.2-500 μg/mL. The intra-batch precision (R.S.D.) was less than 10.6% and inter-batch R.S.D. was less than 11.2%, while accuracy was with ±8% and ±9.9%, determined from QC samples for flucloxacillin and ampicillin. A rapid, sensitive and specific method for simultaneous quantifying flucloxacillin and ampicillin in human plasma have been devised and successfully applied to a clinic pharmacokinetic study.
authors: Clinical Pharmacology Research Laboratory, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Chenrong
authors: C
authors: Huang
authors: Jie
authors: J
authors: Gao
authors: Liyan
authors: L
authors: Miao
mesh_terms: D000328:Adult; D000667:Ampicillin / Q000097:blood* / Q000493:pharmacokinetics; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D005436:Floxacillin / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D005436:Floxacillin; D000667:Ampicillin