title: Electropolymerized poly(Toluidine blue)-modified carbon felt for highly sensitive amperometric determination of NADH in flow injection analysis.
abstract: Poly(pheniothiazine) films were prepared on a porous carbon felt (CF) electrode surface by an electrooxidative polymerization of three phenothiazine derivatives (i.e.,Tthionine (TN), Toluidine Blue (TB) and Methylene Blue (MB)) from 0.1 mol/L phosphate buffer solution (pH 7.0). Among the three phenothiazies, the poly(TB) film-modified CF exhibited an excellent electrocatalytic activity for the oxidation of nicotinamide adenine dinucleotide reduced form (NADH) at +0.2 V vs. Ag/AgCl. The poly(TB) film-modified CF was successfully used as working electrode unit of highly sensitive amperometric flow-through detector for NADH. The peak currents (peak heights) were almost unchanged, irrespective of a carrier flow rate ranging from 2.0 to 4.1 mL/min, resulting in the measurement of NADH (ca. 30 samples/hr) at 4.1 mL/min. The peak current responses of NADH showed linear relationship over the concentration range from 1 to 30 micromol/L (sensitivity: 0.318 microA/(micromol/L); correlation coefficient: 0.997). The lower detection limit was found to be 0.3 micromol/L (S/N = 3).
authors: Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan. hasebe@sit.ac.jp
authors: Yasushi
authors: Y
authors: Hasebe
authors: Yue
authors: Y
authors: Wang
authors: Kazuya
authors: K
authors: Fukuoka
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods*; D009243:NAD / Q000032:analysis*; D010084:Oxidation-Reduction; D010640:Phenothiazines / Q000737:chemistry; D014048:Tolonium Chloride / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D010640:Phenothiazines; D009243:NAD; D014048:Tolonium Chloride