title: [Safety evaluation and quality control of MSCs from hepatitis B patient in vitro].
abstract: OBJECTIVE
  To get mesenchymal stem cells (MSCs) from hepatitis B patient and to valuate the safety and quality after long-term culture in vitro.
  
  
  METHODS
  The cells obtained directly from bone marrow and cultured in Mesen Pro medium supplemented with FGF, and the morphology of MSCs was observed. Surface antigens of the MSCs were analyzed by flow-cytometry. The bacteria, virus, endotoxin and residual serum of cell suspension were detected. The MSCs and perpheral blood T lymphocytes were co-cultured in 48 well plates for 72 h and the T lymphocyte proliferation was measured by using MTT reduction method and the effect of MSCs on T lymphocyte transformation stimulated by PHA was also observed. The oncogenicity of MSCs was verified by the tumorigenesis test in sofo agar. The genetic stability of MSCs was examined by karyotype analysis.
  
  
  RESULTS
  The MSCs from hepatitis B patient could be passaged to many generations and had strong abilities of proliferation. They expressed stem cell-surface antigens and maintained normal karyotype, prevented the pollution of bacteria and viruses, inhibited the immune response of allogenic T lymphocytes and no oncogenicity found.
  
  
  CONCLUSIONS
  The MSCs have proliferative potentials, can be passaged in long-term cultures in Mesen Pro medium without oncogenicity, can maintain normal karyotype, can inhibit the immune response of T lymphocytes and can alleviate the grafe-versus diseases. The MSCs can be served as a new type of cells in cell and gene therapy.
authors: Department of Infectious Diseases, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors: Yang
authors: Y
authors: Zhang
authors: Liang
authors: L
authors: Peng
authors: Chan
authors: C
authors: Xie
authors: Bing-Liang
authors: BL
authors: Lin
authors: Yang-Su
authors: YS
authors: Huang
authors: Jun-Qiang
authors: JQ
authors: Xie
authors: Zhi-Liang
authors: ZL
authors: Gao
authors: Guang-Han
authors: GH
authors: Luo
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006509:Hepatitis B; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011786:Quality Control; D013601:T-Lymphocytes / Q000166:cytology