title: MicroRNA-15b is induced with E2F-controlled genes in HPV-related cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are important regulators of cellular processes and are found to be deregulated in many cancers. We here analysed the miRNA expression in anal carcinomas. In a previous study, we found that our anal carcinoma tumours were divided into two groups based on the expression of E2F-regulated genes. Therefore, we searched for miRNAs that could reproduce this grouping.
  
  
  METHODS
  A global screen of the miRNA population was performed using real-time quantitative PCR (RT-qPCR) array methods and differentially expressed miRNAs were identified. Real-time-qPCR was used to verify the expression levels of selected miRNAs and genes in a larger collection of biopsies. A siRNA-mediated knockdown of human papilloma virus (HPV)16 E7 in a cervical cell line was performed to assess the effect of E7 on miR-15b.
  
  
  RESULTS
  The grouping of tumours into two groups based on the expression of E2F-controlled genes was confirmed in a larger collection of anal carcinoma tumours. The expression of miR-15b was shown to be highly correlated with that of five selected E2F-induced genes (CCNA2, CCNB1, CCNB2, MSH6 and MCM7). A knockdown of HPV16 E7 resulted in decreased levels of miR-15b in Ca Ski cells.
  
  
  CONCLUSIONS
  MiR-15b expression correlates with E2F-regulated genes in anal carcinoma and appears to be part of the E2F-regulatory network.
authors: Section of Oncology, Institute of Medicine, University of Bergen, Bergen, Norway.
authors: M P
authors: MP
authors: Myklebust
authors: O
authors: O
authors: Bruland
authors: Ø
authors: Ø
authors: Fluge
authors: A
authors: A
authors: Skarstein
authors: L
authors: L
authors: Balteskard
authors: O
authors: O
authors: Dahl
mesh_terms: D001005:Anus Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D045744:Cell Line, Tumor; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D050684:E2F Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques / Q000379:methods; D005820:Genetic Testing / Q000379:methods; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000378:metabolism; D052161:Human papillomavirus 18 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D019927:Cyclin E; D050684:E2F Transcription Factors; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; C059731:oncogene protein E7, Human papillomavirus type 16