title: Molecular survey and genetic identification of Anaplasma species in goats from central and southern China.
abstract: Anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. Few studies have been carried out on Anaplasma infections in central and southern China. This study was conducted to determine the coinfection rates of Anaplasma ovis, A. bovis, and A. phagocytophilum from 262 field blood samples of goats in these regions. The average prevalences of single infection of A. ovis, A. bovis, and A. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. Coinfection of A. ovis and A. bovis was dominant, with an infection rate of 27.1%. Coinfection of A. ovis and A. phagocytophilum was 1.9% and that of A. bovis and A. phagocytophilum was 4.2%. Three-pathogen coinfection was found in three of four investigated provinces with a prevalence between 0 and 5.3%. The accuracy of the PCR results was corroborated by sequencing. Analysis of the 16S rRNA gene sequences of A. bovis and A. phagocytophilum confirmed the presence of these pathogens at the investigated sites and indicated the possible genetic diversity of A. phagocytophilum. Field blood inoculation of experimental animals led to successful identification and observation of the morphological shapes of A. bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites.
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, People's Republic of China.
authors: Zhijie
authors: Z
authors: Liu
authors: Miling
authors: M
authors: Ma
authors: Zhaowen
authors: Z
authors: Wang
authors: Jing
authors: J
authors: Wang
authors: Yulv
authors: Y
authors: Peng
authors: Youquan
authors: Y
authors: Li
authors: Guiquan
authors: G
authors: Guan
authors: Jianxun
authors: J
authors: Luo
authors: Hong
authors: H
authors: Yin
mesh_terms: D000709:Anaplasma / Q000145:classification* / Q000302:isolation & purification*; D000818:Animals; D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D001431:Bacteriological Techniques / Q000379:methods; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D060085:Coinfection / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D015511:Goat Diseases / Q000453:epidemiology* / Q000382:microbiology*; D006041:Goats; D025202:Molecular Diagnostic Techniques / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S