title: Inhibition of encephalomyocarditis virus replication by shRNA targeting 1D and 3AB genes in vitro and in vivo.
abstract: Encephalomyocarditis virus (EMCV) could infect many host species and cause acute myocarditis and sudden death in pre-weaned piglets. It was necessary to develop new antiviral strategies for the treatment of the virus infection. Here, four plasmids expressing shRNA (small hairpin RNA) targeted to 1D or 3AB protein genes of EMCV were constructed and their inhibition efficiency on the replication of EMCV was evaluated in both BHK21 cells and mice. The results showed that three out of those four shRNA constructs could significantly inhibit EMCV replication in BHK21 cells on the levels of viral RNA and protein. Moreover, it was found that the shRNAs could suppress significantly the load of EMCV in the brain tissue of the mice pretreated with the constructs for 6-24 h. The clinical signs and pathological lesions of the mice in the groups inoculated with the shRNA constructed were milder obviously, compared with those in pSUPER-mN3 and challenge control groups. The survival rates of mice inoculated with pSUPER-3AB-1, pSUPER-3AB-2, and pSUPER-1D-1 for 12 h was 100, 80, and 40%, respectively, while, in the control groups it was only 20%. It indicated that the vector-based shRNA targeting to 3AB and 1D genes might be a potential anti-EMCV strategy.
authors: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors: Juan
authors: J
authors: Bai
authors: Kangfu
authors: K
authors: Jiang
authors: Basit
authors: B
authors: Zeshan
authors: Xianwei
authors: X
authors: Wang
authors: Yufeng
authors: Y
authors: Li
authors: Ping
authors: P
authors: Jiang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000378:metabolism*; D001921:Brain / Q000821:virology; D018188:Cardiovirus Infections / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D002460:Cell Line; D006224:Cricetinae; D004195:Disease Models, Animal; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000254:growth & development*; D015870:Gene Expression; D005814:Genes, Viral; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D016896:Treatment Outcome; D019562:Viral Load; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D034741:RNA, Small Interfering