title: Autoreactivity against myelin basic protein in patients with chronic paraplegia.
abstract: BACKGROUND
  Previous studies have shown the existence of either cellular or humoral MBP-reactive elements up to 5 years after spinal cord injury (SCI), but not the presence of both after 10 years.
  
  
  METHODS
  Twelve SCI patients, with more than 10 years of evolution, and 18 healthy blood donors were studied. Lymphocyte proliferation (colorimetric-BrdU ELISA assay) and antibody titers against MBP (ELISA Human IgG MBP-specific assay) were assessed.
  
  
  RESULTS
  SCI patients presented a significant T-cell proliferation against MBP (lymphocyte proliferation index: 3.7 ± 1.5, mean ± SD) compared to control individuals (0.7 ± 0.3; P < 0.001). Humoral response analysis yielded a significant difference (P < 0.0001) between the antibody titers of controls and SCI patients. A significant correlation between cellular and humoral responses was observed. Finally, patients with an ASIA B presented the highest immune responses.
  
  
  CONCLUSIONS
  This work demonstrates, for the first time, the existence of both cellular and humoral responses against MBP in the chronic stages (>10 years) of injury.
authors: Facultad de Ciencias de la Salud, Universidad Anáhuac México Norte, Huixquilucan, Edo. de México, Mexico.
authors: D
authors: D
authors: Zajarías-Fainsod
authors: J
authors: J
authors: Carrillo-Ruiz
authors: H
authors: H
authors: Mestre
authors: I
authors: I
authors: Grijalva
authors: I
authors: I
authors: Madrazo
authors: A
authors: A
authors: Ibarra
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood; D016022:Case-Control Studies; D049109:Cell Proliferation; D002908:Chronic Disease; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D004676:Myelin Basic Protein / Q000276:immunology*; D010264:Paraplegia / Q000097:blood / Q000209:etiology / Q000276:immunology*; D013119:Spinal Cord Injuries / Q000097:blood / Q000150:complications / Q000276:immunology*; D013601:T-Lymphocytes / Q000473:pathology; D013997:Time Factors
chemical_list: D000906:Antibodies; D004676:Myelin Basic Protein