title: Transcriptional regulation of type I interferon gene expression by interferon regulatory factor-3 in Japanese flounder, Paralichthys olivaceus.
abstract: Type I interferon (IFN) induces the antiviral response in innate immunity. The type I IFN gene cloned from Japanese flounder (Paralichthys olivaceus) has a length of 1189 bp and consisting of 5 exons and 4 introns. In a phylogenetic tree of type I IFNs, Japanese flounder grouped with other Acanthopterygii. To gain insight into the transcriptional regulation of IFN gene, the 1.36 kb 5'-upstream region including numerous canonical motifs to bind transcription factors [for example, IFN regulatory factor (IRF)] was analyzed. In HINAE cells using a luciferase reporter assay, poly I:C-responsive transcriptional activity was found in the region from -634 to -179 bp. This region includes several IRF motifs. In the presence of poly I:C, overexpression of IRF3 and RLR strongly enhanced transcriptional activity. These results suggest that the transcriptional regulation of Japanese flounder type I IFN is regulated by IRF3 after triggering with dsRNA sensors.
authors: Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-710, South Korea.
authors: Maki
authors: M
authors: Ohtani
authors: Jun-ichi
authors: J
authors: Hikima
authors: Seong Don
authors: SD
authors: Hwang
authors: Takahiro
authors: T
authors: Morita
authors: Yoshiaki
authors: Y
authors: Suzuki
authors: Goshi
authors: G
authors: Kato
authors: Hidehiro
authors: H
authors: Kondo
authors: Ikuo
authors: I
authors: Hirono
authors: Tae-Sung
authors: TS
authors: Jung
authors: Takashi
authors: T
authors: Aoki
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005091:Exons; D005432:Flounder / Q000378:metabolism*; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism*; D007370:Interferon Type I / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D050838:Interferon Regulatory Factor-3; D007370:Interferon Type I; D018168:Receptors, Retinoic Acid