title: Choristoneura fumiferana multiple nucleopolyhedrovirus LEF-3-P143 complex can complement DNA replication and budded virus in an AcMNPV LEF-3-P143 double knockout bacmid.
abstract: Transient replication assays using Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Choristoneura fumiferana multiple nucleopolyhedrovirus (CfMNPV) genes suggested that the interactions between P143, the viral helicase and LEF-3, a ssDNA-binding protein, may represent virus species specificity determinants. P143 and LEF-3 are essential for DNA replication in these assays and together with IE-1, the major immediate-early transcription factor, may be part of the viral replisome. In the current report, a lef-3/p143 double-knockout AcMNPV bacmid was constructed that was defective for viral DNA replication and late gene expression. When the homologous lef-3/p143 CfMNPV genes were introduced into this double-knockout bacmid, DNA replication was restored but the level of replication was lower, budded virus production was delayed, and the yields were reduced from those in an AcMNPV-rescue bacmid. These results suggest that to maximize virus replication, baculovirus replisome assembly and function requires protein-protein interactions between P143 and LEF-3, and other viral proteins.
authors: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
authors: Mei
authors: M
authors: Yu
authors: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
authors: Eric B
authors: EB
authors: Carstens
mesh_terms: D000818:Animals; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D007915:Lepidoptera / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000254:growth & development*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004265:DNA Helicases