title: MnSOD expression inhibited by electromagnetic pulse radiation in the rat testis.
abstract: Male Sprague Dawley rats were exposed to EMP irradiation of 100 kV/m peak-to-peak e-field intensity and different numbers of pulses. Rat sperm samples were prepared for analysis of sperm qualities; Testes were assessed by transmission electron microscopy and serum hormone concentrations were examined by radioimmunoassay; Enzymatic activities of Total-superoxide dismutase(T-SOD) and manganese-superoxide dismutase (MnSOD), the mRNA levels of MnSOD and cuprozinc-superoxide dismutase (CuZnSOD), and the density of malondialdehyde (MDA) were also determined. EMP irradiation did not affect spermatozoon morphology, micronucleus formation rate, sperm number or viability, but the acrosin reaction rate decreased at 24 h and 48 h and recovered by 72 h after irradiation as compared to the controls. The ultrastructure of rat testis displayed more serious damage at 24 h than at other time points (6 h, 12 h, 48 h). Serum levels of luteotrophic hormone (LH) and testosterone (T) were elevated in irradiated rats as compared to controls. After irradiation, enzymatic activities of T-SOD and MnSOD were reduced by 24 h, consistent with the changes observed in MnSOD mRNA expression; MDA content increased at 6 h in turn. These studies have quantified the morphological damage and dysfunction in the rat reproductive system induced by EMP. The mechanism of EMP induced damage may be associated with the inhibition of MnSOD expression.
authors: Department of Radiation Medicine, The Fourth Military Medical University, Xi'an, China.
authors: LiHua
authors: L
authors: Zeng
authors: XiTuan
authors: X
authors: Ji
authors: YanJun
authors: Y
authors: Zhang
authors: Xia
authors: X
authors: Miao
authors: ChangXu
authors: C
authors: Zou
authors: HaiYang
authors: H
authors: Lang
authors: Jie
authors: J
authors: Zhang
authors: YuRong
authors: Y
authors: Li
authors: XiaoWu
authors: X
authors: Wang
authors: HongXing
authors: H
authors: Qi
authors: DongQin
authors: D
authors: Ren
authors: GuoZhen
authors: G
authors: Guo
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D060733:Electromagnetic Radiation; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007986:Luteinizing Hormone / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D046529:Microscopy, Electron, Transmission; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D013737:Testis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*
chemical_list: D007986:Luteinizing Hormone; D013482:Superoxide Dismutase