title: SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion.
abstract: Bone marrow mesenchymal stem cells (MSCs) are considered as a promising cell source to treat the acute myocardial infarction. However, over 90% of the stem cells usually die in the first three days of transplantation. Survival potential, migration ability and paracrine capacity have been considered as the most important three factors for cell transplantation in the ischemic cardiac treatment. We hypothesized that stromal-derived factor-1 (SDF-1)/CXCR4 axis plays a critical role in the regulation of these processes. In this study, apoptosis was induced by exposure of MSCs to H(2)O(2) for 2 h. After re-oxygenation, the SDF-1 pretreated MSCs demonstrated a significant increase in survival and proliferation. SDF-1 pretreatment also enhanced the migration and increased the secretion of pro-survival and angiogenic cytokines including basic fibroblast growth factor and vascular endothelial growth factor. Western blot and RT-PCR demonstrated that SDF-1 pretreatment significantly activated the pro-survival Akt and Erk signaling pathways and up-regulated Bcl-2/Bax ratio. These protective effects were partially inhibited by AMD3100, an antagonist of CXCR4.We conclude that the SDF-1/CXCR4 axis is critical for MSC survival, migration and cytokine secretion.
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
authors: Xiaolei
authors: X
authors: Liu
authors: Biyan
authors: B
authors: Duan
authors: Zhaokang
authors: Z
authors: Cheng
authors: Xiaohua
authors: X
authors: Jia
authors: Lina
authors: L
authors: Mao
authors: Hao
authors: H
authors: Fu
authors: Yongzhe
authors: Y
authors: Che
authors: Lailiang
authors: L
authors: Ou
authors: Lin
authors: L
authors: Liu
authors: Deling
authors: D
authors: Kong
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology*; D015687:Cell Hypoxia; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D015870:Gene Expression; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D016207:Cytokines; D019718:Receptors, CXCR4; D007770:L-Lactate Dehydrogenase; D051057:Proto-Oncogene Proteins c-akt