title: Rapamycin inhibiting Jurkat T cells viability through changing mRNA expression of serine/threonine protein phosphatase 2A.
abstract: OBJECTIVE
  In this study, we analyzed the mRNA expression of serine/threonine (Ser/Thr) protein phosphatase 2A (PP2A) in the human leukemic T-cell line Jurkat cells treated with rapamycin, to determine whether rapamycin inhibiting cell viability is accompanied with the change of mRNA expression of PP2A.
  
  
  RESULTS
  Jurkat cells were incubated with various concentrations of rapamycin and cultured for different hours. Cell viability was assessed by MTT assay. The mRNA expressions of PP2A subunits were measured by quantitative real-time polymerase chain reaction (PCR). We found that rapamycin had an inhibitory effect on cell viability. IC50 was 343.3 nM at 48 h.We also found rapamycin had a dose and time-dependent effect on the gene expression of PP2A. When setting the concentration of rapamycin 500 nM, the mRNA expressions of PP2A subunits (Aa, Aβ, PR55a, PR55δ, PR61γ, PR70, Ca and Cβ) were declined significantly at 48 h. When treated with various concentrations of rapamycin for 48 h, the mRNA expressions of PP2A subunits were down-regulated in the range from 10 nM to 500 nM.
  
  
  CONCLUSIONS
  Rapamycin inhibiting Jurkat T cells viability may be related to the reduction of PP2A mRNA expressions.
authors: Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, 310003, PR China. 1wangbaobao@163.com
authors: Baobao
authors: B
authors: Wang
authors: Qiang
authors: Q
authors: He
authors: Youyin
authors: Y
authors: Mao
authors: Zhimin
authors: Z
authors: Chen
authors: Hong
authors: H
authors: Jiang
authors: Jianghua
authors: J
authors: Chen
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D019169:Jurkat Cells; D010766:Phosphorylation / Q000187:drug effects; D054648:Protein Phosphatase 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects*; D020123:Sirolimus / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
chemical_list: D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D054648:Protein Phosphatase 2; D020123:Sirolimus