title: Crosstalk between blue-light- and ABA-signaling pathways in stomatal guard cells.
abstract: We recently established an immunohistochemical method for the detection of blue light (BL)-induced and phototropin-mediated phosphorylation of plasma-membrane H+-ATPase in stomatal guard cells of Arabidopsis thaliana. This technique makes it possible to detect the phosphorylation/activation status of guard-cell H+-ATPase in the epidermis of a single rosette leaf, without the need to prepare guard-cell protoplasts (GCPs) from a large number of plants. Moreover, it can detect guard-cell responses under more natural and stress-free conditions compared to using GCPs. Taking advantage of these properties, we examined the effect of abscisic acid (ABA) on BL-induced phosphorylation of guard-cell H+-ATPase by using ABA-insensitive mutants. This revealed inhibition of BL-induced phosphorylation of guard-cell H+-ATPase via the early ABA-signaling components PYR/PYL/RCAR-PP2Cs-SnRK2s, which are known to be early ABA-signaling components for a wide range of ABA responses in plants.
authors: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Japan.
authors: Maki
authors: M
authors: Hayashi
authors: Toshinori
authors: T
authors: Kinoshita
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000378:metabolism; D008027:Light; D009154:Mutation; D010766:Phosphorylation; D054046:Plant Stomata / Q000378:metabolism* / Q000528:radiation effects*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000187:drug effects; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D000040:Abscisic Acid; D006180:Proton-Translocating ATPases