title: Arrhythmogenic consequences of myofibroblast-myocyte coupling.
abstract: OBJECTIVE
  Fibrosis is known to promote cardiac arrhythmias by disrupting myocardial structure. Given recent evidence that myofibroblasts form gap junctions with myocytes at least in co-cultures, we investigated whether myofibroblast-myocyte coupling can promote arrhythmia triggers, such as early afterdepolarizations (EADs), by directly influencing myocyte electrophysiology.
  
  
  RESULTS
  Using the dynamic voltage clamp technique, patch-clamped adult rabbit ventricular myocytes were electrotonically coupled to one or multiple virtual fibroblasts or myofibroblasts programmed with eight combinations of capacitance, membrane resistance, resting membrane potential, and gap junction coupling resistance, spanning physiologically realistic ranges. Myocytes were exposed to oxidative (1 mmol/L H(2)O(2)) or ionic (2.7 mmol/L hypokalaemia) stress to induce bradycardia-dependent EADs. In the absence of myofibroblast-myocyte coupling, EADs developed during slow pacing (6 s), but were completely suppressed by faster pacing (1 s). However, in the presence of myofibroblast-myocyte coupling, EADs could no longer be suppressed by rapid pacing, especially when myofibroblast resting membrane potential was depolarized (-25 mV). Analysis of the myofibroblast-myocyte virtual gap junction currents revealed two components: an early transient-outward I(to)-like current and a late sustained current. Selective elimination of the I(to)-like component prevented EADs, whereas selective elimination of the late component did not.
  
  
  CONCLUSIONS
  Coupling of myocytes to myofibroblasts promotes EAD formation as a result of a mismatch in early vs. late repolarization reserve caused by the I(to)-like component of the gap junction current. These cellular and ionic mechanisms may contribute to the pro-arrhythmic risk in fibrotic hearts.
authors: UCLA Cardiovascular Research Laboratory, Division of Cardiology, Department of Medicine, Los Angeles, CA, USA.
authors: Thao P
authors: TP
authors: Nguyen
authors: Yuanfang
authors: Y
authors: Xie
authors: Alan
authors: A
authors: Garfinkel
authors: Zhilin
authors: Z
authors: Qu
authors: James N
authors: JN
authors: Weiss
mesh_terms: D000200:Action Potentials; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology*; D001919:Bradycardia / Q000503:physiopathology; D017629:Gap Junctions / Q000502:physiology; D008297:Male; D032383:Myocytes, Cardiac / Q000502:physiology*; D058628:Myofibroblasts / Q000502:physiology*; D011817:Rabbits