title: Karyotyping of barley chromosomes by a new fluorescence banding technique combined with scanning probe microscopy.
abstract: Fluorescence banding has been used to classify chromosomes, except those of barley. Four of the seven barley chromosomes are indistinguishable by length or arm ratio. C-banding has been used for classification; however, it requires a long aging period. Here, we describe a new fluorescence banding method for barley. The chromosomes are treated with warm acetate followed by staining with a fluorescent dye, YOYO-1. Using this method, all seven barley chromosomes can be clearly distinguished. Atomic force microscopy and scanning near-field microscopy analyses revealed that the surfaces of the banded chromosomes were flat, indicating that the fluorescence intensity reflected the internal DNA density or condensation of chromatin.
authors: Nanobiotechnology Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. ssugi@affrc.go.jp
authors: Shigeru
authors: S
authors: Sugiyama
authors: Tomoyuki
authors: T
authors: Yoshino
authors: Tamaki
authors: T
authors: Hirose
authors: Toshio
authors: T
authors: Ohtani
mesh_terms: D001399:Azure Stains / Q000378:metabolism; D002871:Chromosome Banding / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics* / Q000378:metabolism; D001467:Hordeum / Q000235:genetics*; D059785:Karyotype; D007621:Karyotyping / Q000379:methods*; D008677:Metaphase; D008856:Microscopy, Fluorescence / Q000379:methods; D020527:Microscopy, Scanning Probe / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001399:Azure Stains