title: Dihydrotestosterone inhibits interleukin-1α or tumor necrosis factor α-induced proinflammatory cytokine production via androgen receptor-dependent inhibition of nuclear factor-κB activation in rheumatoid fibroblast-like synovial cell line.
abstract: Rheumatoid arthritis (RA) is a disease with significant gender differences in its prevalence and clinical features. Interleukin (IL)-1 and tumor necrosis factor (TNF) α produced by synoviocytes are principle inflammatory and destructive mediators of RA. We found that a potent androgen, 5α-dihydrotestosterone (DHT) inhibits IL-1α-induced production and mRNA expression of IL-8, IL-6 and IL-1β from RA patient-derived fibroblast-like synovial cell line MH7A. Promoter analysis of the IL-8 gene revealed that nuclear factor (NF)-κB activation is critical for its transcriptional activation by IL-1α, and DHT inhibited the IL-1α-induced NF-κB activation in a manner dependent on the androgen receptor (AR). DHT also inhibited the effects of TNFα on the cells overexpressed with AR, indicating that sufficient expression level of functional AR was necessary for the inhibitory effect of DHT on TNFα. These results suggest that androgen contributes to the prevention against RA and its gender difference by inhibiting IL-1α or TNFα-induced proinflammatory cytokine production from synovial fibroblast-like cells by inhibiting NF-κB activation in a manner depending on AR.
authors: Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3–1 Tanabe-dori, Mizuho-ku, Nagoya, Japan.
authors: Jian
authors: J
authors: Xu
authors: Yuka
authors: Y
authors: Itoh
authors: Hidetoshi
authors: H
authors: Hayashi
authors: Takemasa
authors: T
authors: Takii
authors: Keiji
authors: K
authors: Miyazawa
authors: Kikuo
authors: K
authors: Onozaki
mesh_terms: D000728:Androgens / Q000378:metabolism / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D013196:Dihydrotestosterone / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053582:Interleukin-1alpha / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism*; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000728:Androgens; D018836:Inflammation Mediators; D053582:Interleukin-1alpha; D016209:Interleukin-8; D007378:Interleukins; D016328:NF-kappa B; D012333:RNA, Messenger; D011944:Receptors, Androgen; D014409:Tumor Necrosis Factor-alpha; D013196:Dihydrotestosterone