title: Correlation between expression of cellular retinol-binding protein 1 and its methylation status in larynx cancer.
abstract: OBJECTIVE
  The authors have previously reported that cellular retinol-binding protein 1 (CRBP1) gene gain and its expression correlated significantly with survival in laryngeal carcinoma patients. The authors hypothesised that inactivation of the CRBP1 gene through CpG methylation is associated with patient status and gene expression. In this work, the authors determine the expression and methylation status of the CRBP1 gene and its correlation with clinical variables of laryngeal carcinoma patients.
  
  
  METHODS
  The CRBP1 gene methylation promoter was assessed by methylation specific PCR analysis in tissue samples from larynx cancer specimens and its expression was assessed by immunohistochemistry on paraffin embedded tissue using tissue microarray. The results were then compared with the clinical pathological variables and outcome measures. The study included 46 samples from patients with non-metastatic squamous cell carcinoma of the larynx without any previous oncological treatments.
  
  
  RESULTS
  Lack of CRBP1 expression was seen in 17 of the 46 laryngeal carcinoma samples, while the remaining 29 samples showed increased expression. Significant associations were found between CRBP1 expression and methylation and patient status. There was a tendency for association in all clinical stages of the disease. CRBP1 gene expression and its unmethylated promoter correlated significantly with survival (p<0.05).
  
  
  CONCLUSIONS
  An early event of larynx cancer could be CRBP1 expression related to unmethylation of the promoter region. These features could also be associated with good response and survival. The authors hypothesised that increased expression and unmethylated promoter of the CRBP1 gene could be considered as markers for larynx cancer.
authors: Laboratorio de Oncogenomica, UIMEO, Hospital de Oncología, CMN-SXXI, IMSS, México, Distrito Federal, Mexico.
authors: Raúl
authors: R
authors: Peralta
authors: Alejandra
authors: A
authors: Valdivia
authors: Isabel
authors: I
authors: Alvarado-Cabrero
authors: Francisco
authors: F
authors: Gallegos
authors: Teresa
authors: T
authors: Apresa
authors: Dulce
authors: D
authors: Hernández
authors: Mónica
authors: M
authors: Mendoza
authors: Pablo
authors: P
authors: Romero
authors: Lucero
authors: L
authors: Paniagua
authors: Miguel
authors: M
authors: Ibáñez
authors: Lourdes
authors: L
authors: Cabrera
authors: Mauricio
authors: M
authors: Salcedo
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016009:Chi-Square Distribution; D019175:DNA Methylation; D005260:Female; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D008800:Mexico; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic; D054840:Retinol-Binding Proteins, Cellular / Q000032:analysis / Q000235:genetics*; D015996:Survival Rate; D013997:Time Factors; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C518456:RBP1 protein, human; D054840:Retinol-Binding Proteins, Cellular