title: Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery.
abstract: Nanoscale complexes of recombinant silk molecules containing THPs with DNA are designed as less cytotoxic and highly target-specific gene carriers. Genetically engineered silk proteins containing poly(L-lysine) domains to interact with pDNA and the THP to bind to specific tumorigenic cells for target-specific pDNA delivery are prepared, followed by in vitro transfection into MDA-MB-435 melanoma cells, highly metastatic human breast tumor MDA-MB-231 cells, and non-tumorigenic MCF-10A breast epithelial cells. The silk/poly(L-lysine) block copolymer containing Lyp1 (ML-Lyp1) shows significant differences from silk/poly(L-lysine) block copolymer containing F3 (ML-F3) in cytotoxicity to MCF10A cells. ML-F3 is the most promising candidate for target delivery into tumorigenic cells.
authors: Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
authors: Keiji
authors: K
authors: Numata
authors: Aneta J
authors: AJ
authors: Mieszawska-Czajkowska
authors: Laura A
authors: LA
authors: Kvenvold
authors: David L
authors: DL
authors: Kaplan
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000628:therapy; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry; D004847:Epithelial Cells / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry / Q000235:genetics*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008545:Melanoma / Q000628:therapy; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012878:Skin Neoplasms / Q000628:therapy; D014407:Tumor Cells, Cultured
chemical_list: D004337:Drug Carriers; D058726:Nanoconjugates; D010455:Peptides; D011994:Recombinant Proteins; D011107:Polylysine; D005349:Fibroins