title: Use of a calcium-sensitive electrode for studies on mitochondrial calcium transport.
abstract: Ca(2+)-sensitive electrode as a practical approach is used to follow Ca(2+) changes in the medium and particularly useful to study mitochondrial Ca(2+) uptake (or release); this method permits the continuous recording of Ca(2+) movements through the mitochondrial inner membrane. In this chapter, it is described how to prepare a Ca(2+)-sensitive electrode, and its application on mitochondrial studies with emphasis on the mitochondrial permeability transition.
authors: Department of Life Sciences, IMAR, University of Coimbra, Apartado 3046, 3001-401 Coimbra, Portugal. moreno@ci.uc.pt
authors: António J M
authors: AJ
authors: Moreno
authors: Joaquim A
authors: JA
authors: Vicente
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D017136:Ion Transport; D017736:Ion-Selective Electrodes; D008930:Mitochondria, Liver / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002118:Calcium