title: Chemiluminescent detection of carbohydrates in the tumoral breast diseases.
abstract: Nowadays, there is an increase of investigations into the fibroadenoma, mainly because some studies have shown that the occurrence of fibroadenoma is linked to an increased risk of developing breast carcinoma. Currently, the chemiluminescence biomarkers are applied for validation methods and screening. Here, a lectin chemiluminescence is proposed as new histochemistry method to identify carbohydrates in mammary tumoral tissues. The lectins concanavalin A (Con A) and peanut agglutinin (PNA) conjugated to acridinium ester were used to characterize the glycocode of breast tissues: normal, fibroadenoma, and invasive duct carcinoma (IDC). The lectin chemiluminescence expressed in relative light units (RLU) was higher in fibroadenoma and IDC than in normal tissue for both lectins tested. The relationship RLU emission versus tissue area described a linear and hyperbolic curve for IDC and fibroadenoma, respectively, using Con A whereas hyperbolic curves for both transformed tissues using PNA. RLU was abolished by inhibiting the interaction between tissues and lectins using their specific carbohydrates: methyl-α-D: -mannoside (Con A) and galactose (PNA). The intrinsic fluorescence emission did not change with combination of the lectins (Con A/PNA) to the acridinium ester for hydrophobic residues. These results represent the lectin chemiluminescence as an alternative of histochemistry method for tumoral diagnosis in the breast.
authors: Laboratório de Imunopatologia Keizo Asami, LIKA/UFPE, Recife, Brazil. vanessabrustein@gmail.com
authors: Vanessa Passos
authors: VP
authors: Brustein
authors: Carmelita Lima Bezerra
authors: CL
authors: Cavalcanti
authors: Mario Ribeiro
authors: MR
authors: de Melo-Junior
authors: Maria Tereza Santos
authors: MT
authors: Correia
authors: Eduardo Isidoro Carneiro
authors: EI
authors: Beltrão
authors: Luiz Bezerra
authors: LB
authors: Carvalho
mesh_terms: D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D018226:Fibroadenoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008875:Middle Aged; D019887:Peanut Agglutinin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002241:Carbohydrates; D019887:Peanut Agglutinin; D003208:Concanavalin A