title: Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective.
abstract: G protein-Coupled Receptors (GPCRs) use a complex series of intramolecular conformational changes to couple agonist binding to the binding and activation of cognate heterotrimeric G protein (Gαβγ). The mechanisms underlying this long-range activation have been identified using a variety of biochemical and structural approaches and have primarily used visual signal transduction via the GPCR rhodopsin and cognate heterotrimeric G protein transducin (G(t)) as a model system. In this chapter, we review the methods that have revealed allosteric signaling through rhodopsin and transducin. These methods can be applied to a variety of GPCR-mediated signaling pathways.
authors: Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN, USA.
authors: Tarjani M
authors: TM
authors: Thaker
authors: Ali I
authors: AI
authors: Kaya
authors: Anita M
authors: AM
authors: Preininger
authors: Heidi E
authors: HE
authors: Hamm
authors: T M
authors: TM
authors: Iverson
mesh_terms: D000494:Allosteric Regulation / Q000502:physiology; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D017433:Protein Structure, Secondary; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism*; D012243:Rhodopsin / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D015293:Transducin / Q000737:chemistry / Q000378:metabolism
chemical_list: D043562:Receptors, G-Protein-Coupled; D012243:Rhodopsin; D020962:Heterotrimeric GTP-Binding Proteins; D015293:Transducin