title: CD83-stimulated monocytes suppress T-cell immune responses through production of prostaglandin E2.
abstract: CD83 is commonly known as a specific marker for mature dendritic cells. It has been shown to be important for CD4(+) T-cell development in the thymus. However, its function in the peripheral immune system remains enigmatic. Here, we show that CD83 inhibits proliferation and production of IL-2 and IFN-γ by T cells, and the inhibitory effect of CD83 is mediated by monocytes. Prostaglandin E2 (PGE(2)), but not IL-10 or TGF-β, was up-regulated specifically by CD83 in monocytes. Consistent with high levels of PGE(2), expression of COX-2 also was increased upon CD83 treatment. NF-κB activation also is required for induction of PGE(2) by CD83. Finally, application of the COX-2-selective inhibitor NS-398 fully prevented CD83-triggered inhibition of T-cell responses. Our study establishes an immune-regulatory mechanism by CD83 via stimulation of PGE(2) production in monocytes.
authors: Institute of Medical Biotechnology and Jiangsu Stem Cell Key Laboratory,  Soochow University, Suzhou 215007,  China.
authors: Liwen
authors: L
authors: Chen
authors: Yibei
authors: Y
authors: Zhu
authors: Guangbo
authors: G
authors: Zhang
authors: Chao
authors: C
authors: Gao
authors: Weixue
authors: W
authors: Zhong
authors: Xueguang
authors: X
authors: Zhang
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology; D015232:Dinoprostone / Q000378:metabolism*; D006801:Humans; D007107:Immune System; D007136:Immunoglobulins / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology*; D016328:NF-kappa B / Q000378:metabolism; D009578:Nitrobenzenes / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013449:Sulfonamides / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014157:Transcription Factors / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D015232:Dinoprostone; D007136:Immunoglobulins; D007371:Interferon-gamma; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D009578:Nitrobenzenes; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D014157:Transcription Factors; D000097802:CD83 Antigen; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide