title: Sarcomere length determination using front-face fluorescence polarization.
abstract: Tryptophan is the major intrinsic fluorophore in muscle and is a constituent of proteins that have two preferential alignments both parallel and perpendicular to muscle fibre direction. A simple theoretical model and an experimental method based on front-face fluorescence polarization technique for tryptophan fluorescence anisotropy measurements were used for the estimation of post-rigor sarcomere length in beef in the range 1.6-3.4μm. Fluorescence anisotropy and structure-related model variables displayed changes in cold-shortened samples compared with normal and stretched ones. The anisotropy of contracted samples was lowered by misalignment of fibres in the sample. This method can therefore be used for in-line detection of cold shortening which has meat toughness as a consequence.
authors: Centre INRA de Theix, UR370 QuaPA, F-63122 Saint-Genes-Champanelle, France.
authors: C
authors: C
authors: Luc
authors: S
authors: S
authors: Clerjon
authors: F
authors: F
authors: Peyrin
authors: J
authors: J
authors: Lepetit
authors: J
authors: J
authors: Culioli