title: Gonadal status of male recipient mice influences germ cell development in immature buffalo testis tissue xenograft.
abstract: Growth and development of immature testis xenograft from various domestic mammals has been shown in mouse recipients; however, buffalo testis xenografts have not been reported to date. In this study, small fragments of testis tissue from 8-week-old buffalo calves were implanted subcutaneously onto the back of immunodeficient male mouse recipients, which were either castrated or left intact (non-castrated). The xenografts were retrieved and analyzed 12 and 24 weeks later. The grafted tissue survived and grew in both types of recipient with a significant increase in weight and seminiferous tubule diameter. Recovery of grafts from intact recipients 24 weeks post-grafting was significantly lower than that from the castrated recipients. Seminal vesicle indices and serum testosterone levels were lower in castrated recipients at both collection time points in comparison to the intact recipients and non-grafted intact mouse controls. Pachytene spermatocytes were the most advanced germ cells observed in grafts recovered from castrated recipients 24 weeks post-grafting. Complete spermatogenesis, as indicated by the presence of elongated spermatids, was present only in grafts from intact recipients collected 24 weeks post-grafting. However, significant number of germ cells with DNA damage was also detected in these grafts as indicated by TUNEL assay. The complete germ cell differentiation in xenografts from intact recipients may be attributed to efficient Sertoli cell maturation. These results suggest that germ cell differentiation in buffalo testis xenograft can be completed by altering the recipient gonadal status.
authors: Laboratory for the Conservation of Endangered Species, Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad, India.
authors: Niranjan
authors: N
authors: Reddy
authors: Ranjeet Singh
authors: RS
authors: Mahla
authors: Revanth
authors: R
authors: Thathi
authors: Sanjay Kumar
authors: SK
authors: Suman
authors: Jedy
authors: J
authors: Jose
authors: Sandeep
authors: S
authors: Goel
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D002020:Buffaloes; D002454:Cell Differentiation; D004249:DNA Damage; D006085:Graft Survival; D064593:Heterografts; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009919:Orchiectomy; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012669:Seminal Vesicles / Q000254:growth & development; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000254:growth & development / Q000502:physiology / Q000637:transplantation*; D013739:Testosterone / Q000097:blood; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D013739:Testosterone; D054304:Anti-Mullerian Hormone; D043222:Ubiquitin Thiolesterase