title: β-1,3-Glucan recognition protein (βGRP) is essential for resistance against fungal pathogen and opportunistic pathogenic gut bacteria in Locusta migratoria manilensis.
abstract: Pattern recognition proteins, which form part of the innate immune system, initiate host defense reactions in response to pathogen surface molecules. The pattern recognition protein β-1,3-glucan recognition protein (βGRP) binds to β-1,3-glucan on fungal surfaces to mediate melanization via the prophenoloxidase (PPO)-activating cascade. In this study, cDNA encoding a 53-kDa βGRP (LmβGRP) was cloned from Locusta migratoria manilensis. LmβGRP mRNA shown to be constitutively expressed specifically in hemocytes and was highly upregulated following fungal infection. LmβGRP-silenced (LmβGRP-RNAi) mutant locusts exhibited significantly reduced survival rate following fungal infection (Metarhizium acridum) compared with the wild-type. Furthermore, LmβGRP-RNAi mutants exhibited abnormally loose stools indicative of a gut defect. 16S rRNA gene analysis detected the opportunistic pathogenic bacterium, Vibrio vulnificus in LmβGRP mutant but not wild-type locusts, suggesting changes in the composition of gut bacterial communities. These results indicate that LmβGRP is essential to gut immunity in L. migratoria manilensis.
authors: Genetic Engineering Research Center, School of Bioengineering, Chongqing Engineering Research Center for Fungal Insecticide, The Key Laboratory of Gene Function and Expression Regulation, Chongqing University Chongqing 400044, China.
authors: Xiaoli
authors: X
authors: Zheng
authors: Yuxian
authors: Y
authors: Xia
mesh_terms: D000818:Animals; D001419:Bacteria; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D005658:Fungi; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology; D019476:Insect Proteins / Q000276:immunology* / Q000378:metabolism; D037102:Lectins / Q000276:immunology* / Q000378:metabolism; D048188:Locusta migratoria / Q000276:immunology* / Q000382:microbiology*; D010802:Phylogeny; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D037102:Lectins; D047071:beta-Glucans; C037937:glucan-binding proteins; C033363:beta-1,3-glucan