title: Bovine muscle shortening and protein degradation after electrical stimulation, excision and chilling.
abstract: Electrical stimulation-dependent improvement in beef tenderness resulted from mechanisms other than avoidance of cold shortening in excised muscle chilled at a normal rate (10°C at 10h post-stimulation). At normal chilling rate, electrical stimulation enhanced degradation of the myofibrillar proteins, alpha actinin and troponin-T, and increased the amount of a 30 000 dalton protein, as assessed by gel electrophoresis, whereas sarcomere lengths were not different from unstimulated muscle. Under slightly accelerated chilling conditions (10°C at 5 h post stimulation), electrical stimulation prevented cold shortening but the meat was more tender than, and had the same sarcomere length as, unstimulated muscle chilled to 10°C in 10 h. Electrical stimulation did not improve the tenderness of beef chilled at a rapid rate (10°C at 2 h post stimulation), nor did it prevent cold shortening when muscles were chilled rapidly.
authors: Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors: C P
authors: CP
authors: Salm
authors: J C
authors: JC
authors: Forrest
authors: E D
authors: ED
authors: Aberle
authors: E W
authors: EW
authors: Mills
authors: A C
authors: AC
authors: Snyder
authors: M D
authors: MD
authors: Judge