title: Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers.
abstract: Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, which along with Sanger-based bacterial artificial chromosome end sequences and a genetic map, we assembled into scaffolds representing 72.7% (605.78 Mb) of the 833.07 Mb pigeonpea genome. Genome analysis predicted 48,680 genes for pigeonpea and also showed the potential role that certain gene families, for example, drought tolerance-related genes, have played throughout the domestication of pigeonpea and the evolution of its ancestors. Although we found a few segmental duplication events, we did not observe the recent genome-wide duplication events observed in soybean. This reference genome sequence will facilitate the identification of the genetic basis of agronomically important traits, and accelerate the development of improved pigeonpea varieties that could improve food security in many developing countries.
authors: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India. r.k.varshney@cgiar.org
authors: Rajeev K
authors: RK
authors: Varshney
authors: Wenbin
authors: W
authors: Chen
authors: Yupeng
authors: Y
authors: Li
authors: Arvind K
authors: AK
authors: Bharti
authors: Rachit K
authors: RK
authors: Saxena
authors: Jessica A
authors: JA
authors: Schlueter
authors: Mark T A
authors: MT
authors: Donoghue
authors: Sarwar
authors: S
authors: Azam
authors: Guangyi
authors: G
authors: Fan
authors: Adam M
authors: AM
authors: Whaley
authors: Andrew D
authors: AD
authors: Farmer
authors: Jaime
authors: J
authors: Sheridan
authors: Aiko
authors: A
authors: Iwata
authors: Reetu
authors: R
authors: Tuteja
authors: R Varma
authors: RV
authors: Penmetsa
authors: Wei
authors: W
authors: Wu
authors: Hari D
authors: HD
authors: Upadhyaya
authors: Shiaw-Pyng
authors: SP
authors: Yang
authors: Trushar
authors: T
authors: Shah
authors: K B
authors: KB
authors: Saxena
authors: Todd
authors: T
authors: Michael
authors: W Richard
authors: WR
authors: McCombie
authors: Bicheng
authors: B
authors: Yang
authors: Gengyun
authors: G
authors: Zhang
authors: Huanming
authors: H
authors: Yang
authors: Jun
authors: J
authors: Wang
authors: Charles
authors: C
authors: Spillane
authors: Douglas R
authors: DR
authors: Cook
authors: Gregory D
authors: GD
authors: May
authors: Xun
authors: X
authors: Xu
authors: Scott A
authors: SA
authors: Jackson
mesh_terms: D036561:Cajanus / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D056916:Segmental Duplications, Genomic; D017422:Sequence Analysis, DNA / Q000379:methods*; D013025:Glycine max / Q000235:genetics; D026801:Synteny / Q000235:genetics
chemical_list: D005819:Genetic Markers