title: Molecular phenotype of monocytes at the maternal-fetal interface.
abstract: OBJECTIVE
  The purpose of this study was to gain insight into the pathways that are associated with inflammation at the maternal-fetal interface. This study examined the molecular characteristics of monocytes that were derived from the maternal circulation and the placenta of obese women.
  
  
  METHODS
  Mononuclear cells were isolated from placenta, venous maternal, and umbilical cord blood at term delivery; activated monocytes were separated with CD14 immunoselection. The genotype and expression pattern of the monocytes were analyzed by microarray and real-time reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  The transcriptome of the maternal blood and placental CD14 monocytes exhibited 73% homology, with 10% (1800 common genes) differentially expressed. Genes for immune sensing and regulation, matrix remodeling, and lipid metabolism were enhanced 2-2006 fold in placenta, compared with maternal monocytes. The CD14 placental monocytes exhibited a maternal genotype (9% DYS14 expression) as opposed to the fetal genotype (90% DYS14 expression) of the trophoblast cells.
  
  
  CONCLUSIONS
  CD14 monocytes from the maternal blood and the placenta share strong phenotypic and genotypic similarities with an enhanced inflammatory pattern in the placenta. The functional traits of the CD14 blood and placental monocytes suggest that they both contribute to propagation of inflammation at the maternal-fetal interface.
authors: Department of Obstetrics and Gynecology, Case Western Reserve University, Cleveland, OH, USA.
authors: Subhabrata
authors: S
authors: Basu
authors: Patrick
authors: P
authors: Leahy
authors: Jean-Claude
authors: JC
authors: Challier
authors: Judi
authors: J
authors: Minium
authors: Patrick
authors: P
authors: Catalano
authors: Sylvie
authors: S
authors: Hauguel-de Mouzon
mesh_terms: D000328:Adult; D005260:Female; D005312:Fetal Blood / Q000276:immunology / Q000378:metabolism*; D005333:Fetus / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D009765:Obesity / Q000276:immunology / Q000378:metabolism; D010920:Placenta / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D059467:Transcriptome
chemical_list: D018950:Lipopolysaccharide Receptors