title: Micromechanical measurement of AChBP binding for label-free drug discovery.
abstract: A potential binding assay based on binding-driven micromechanical motion is described. Acetylcholine binding protein (AChBP) was used to modify a microcantilever. The modified microcantilever was found to bend on application of the naturally occurring agonist (acetylcholine) or the antagonist (nicotine and d-tubocurarine). Control experiments show that microcantilevers modified without AChBP do not respond to acetylcholine, nicotine, and d-tubocurarine. K(d) values obtained for acetylcholine, nicotine, and d-tubocurarine are similar to those obtained from radio-ligand binding assays. These results suggest that the microcantilever system has potential for use in label free, drug screening applications.
authors: Department of Chemistry, Drexel University, Philadelphia, PA 19104, USA.
authors: Koutilya
authors: K
authors: Buchapudi
authors: Xiaohe
authors: X
authors: Xu
authors: Yeganeh
authors: Y
authors: Ataian
authors: Hai-Feng
authors: HF
authors: Ji
authors: Marvin
authors: M
authors: Schulte
mesh_terms: D000109:Acetylcholine / Q000819:agonists / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055808:Drug Discovery / Q000379:methods*; D008024:Ligands; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation / Q000379:methods*; D009538:Nicotine / Q000032:analysis / Q000378:metabolism; D018733:Nicotinic Antagonists / Q000032:analysis / Q000378:metabolism; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods; D013194:Staining and Labeling; D014403:Tubocurarine / Q000032:analysis / Q000378:metabolism
chemical_list: D008024:Ligands; D018733:Nicotinic Antagonists; D009538:Nicotine; D000109:Acetylcholine; D014403:Tubocurarine