title: Mitochondrial permeability transition induced by different concentrations of zinc.
abstract: Zinc is one of the required trace elements in animals, and it serves an important role in biological systems. However, high levels of zinc are poisonous to organisms. So far, there exist conflicting reports about zinc ions-induced mitochondrial permeability transition (MPT). We analyzed the effects of Zn²⁺ on MPT by monitoring mitochondrial swelling with the ultraviolet-visible light absorption spectrum, characterizing the fluidity of the membrane with fluorescence anisotropy, detecting the transmembrane potential (Δψ) with fluorescence intensity, and observing mitochondrial ultrastructure with transmission electron microscopy. Data reveal that low concentrations of zinc ions can trigger MPT while high levels of zinc ions cannot, which implies that zinc ions' toxicity cannot be the result of a single simple mechanism.
authors: State Key Laboratory of Virology, Key Laboratory of Analytical Chemistry for Biology and Medicine-Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
authors: Xiao-Rong
authors: XR
authors: Liu
authors: Jia-Han
authors: JH
authors: Li
authors: Yue
authors: Y
authors: Zhang
authors: Yu-Shu
authors: YS
authors: Ge
authors: Fang-Fang
authors: FF
authors: Tian
authors: Jie
authors: J
authors: Dai
authors: Feng-Lei
authors: FL
authors: Jiang
authors: Yi
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D008560:Membrane Fluidity / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000494:pharmacology*
chemical_list: D015032:Zinc