title: Effects of exposition to polluted environments on blood cells of the fish Prochilodus lineatus.
abstract: In this study, we investigated the effects of contaminated water on the blood parameters of the fish Prochilodus lineatus exposed during 7 and 20 days. Blood was collected with heparinized syringes, and blood smears were prepared and stained with Leishman stain. Slides were examined and photographed using a Leica light microscope. Total white blood cell counts and differential counts of thrombocytes and leukocytes were obtained for statistical analysis. The group exposed to water samples from Lago Azul exhibited an increase in the number of leukocytes and in the total number of white blood cells, suggesting that the chemical contaminants in this environment were acting similarly to antigens in this fish species, causing the proliferation of defense cells. In the group exposed to detergent during 20 days, the number of thrombocytes decreased. These results suggest that the variations in the number of leukocytes were indicators of environmental pollution and that biodegradable detergents may, after a certain time of exposure, affect vital functions in fish, such as coagulation and prevention of infections, which directly involves thrombocytes.
authors: Universidade Estadual Paulista Júlio de Mesquita Filho, UNESP, Campus de Rio Claro, SP, Brazil. brunofp22@hotmail.com
authors: Bruno Fiorelini
authors: BF
authors: Pereira
authors: Rebeca Mamede
authors: RM
authors: Da Silva Alves
authors: Dimitrius Leonardo
authors: DL
authors: Pitol
authors: José Augusto
authors: JA
authors: Senhorini
authors: Rita
authors: R
authors: De Cássia Gimenes De Alcântara Rocha
authors: Flavio Henrique
authors: FH
authors: Caetano
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects*; D060125:Characiformes; D003584:Cytological Techniques; D007958:Leukocyte Count; D008853:Microscopy; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical