title: Association between apolipoprotein E-polymorphism and Ischemic heart disease patients with or without type 2 diabetes mellitus: a preliminary study in Kuwait.
abstract: BACKGROUND
  We investigated the association between apolipoprotein E polymorphism and ischemic heart disease with or without type 2 diabetes in Kuwait and examined the impact of apolipoprotein E polymorphism in diabetic patients.
  
  
  METHODS
  The present study was conducted from January 2005 to June 2006 in the Diabetic Clinic of Al-Amiri and Al-Sabah Hospitals in Kuwait City. Apolipoprotein E polymorphism was assessed in 250 subjects of which 83 were ischemic heart disease patients (41 diabetic and 42 non-diabetic) and 105 were diabetic patients without ischemic heart disease. Results were compared with 62 healthy controls. Apolipoprotein E polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  Apolipoprotein E3 allele was the most commonly occurring form. The frequency of apolipoprotein E4 was higher in ischemic heart disease patients with type 2 diabetes (39%) and the non-diabetic (31%) group, but lower in the diabetic (20%) and control groups (16%).
  
  
  CONCLUSIONS
  Apolipoprotein E4 allele may be related to the development of ischemic heart disease in patients with or without type 2 diabetes in Kuwait.  However, future studies with larger population sizes are needed to establish such relationship.
authors: Department of Applied Medical Sciences, College of Health Sciences, Public Authority for Applied Education and Training, Kuwait.
authors: Hana T
authors: HT
authors: Al-Majed
authors: Jafar A
authors: JA
authors: Qasem
authors: Ahmed K
authors: AK
authors: Al-Sherifi
authors: Abdulnabi A
authors: AA
authors: Al-Attar
authors: Abdulla A
authors: AA
authors: Qasem
authors: Salwa A
authors: SA
authors: Abdullah
mesh_terms: D000483:Alleles; D053329:Apolipoprotein E2 / Q000235:genetics; D053318:Apolipoprotein E3 / Q000235:genetics; D053327:Apolipoprotein E4 / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007730:Kuwait; D008297:Male; D017202:Myocardial Ischemia / Q000208:ethnology / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E