title: Human cytomegalovirus glycoprotein B genotypes in Chinese hematopoietic stem cell transplant recipients.
abstract: OBJECTIVE
  To investigate the distribution of human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and to explore the possible relationship between gB genotypes and clinical characteristics in Chinese hematopoietic stem cell transplant (HSCT) recipients.
  
  
  METHODS
  A prospective analysis of gB genotypes was conducted on HCMV clinical isolates obtained from 102 HSCT recipients. Real-time quantitative PCR and PCR-based restriction fragment length polymorphism analysis were applied for the determination of viral loads and gB genotypes, respectively.
  
  
  RESULTS
  The distribution of gB genotypes was as follows: gB1, 54/102 (52.9%); gB3, 21/102 (20.6%); and mixtures, 27/102 (26.5%). The rate of viral clearance at day 21 was higher in patients infected with the gB1 genotype than in those infected with the gB3 genotype (56 and 29%, respectively; p = 0.036). In contrast, the rate of HCMV reactivation/reinfection was higher in patients infected with the gB3 genotype than in those infected with the gB1 genotype (81 and 56%, respectively; p = 0.041).
  
  
  CONCLUSIONS
  The HCMV gB1 genotype is the most prevalent among Chinese HSCT recipients; patients infected with the gB3 genotype have more difficulty eradicating the virus and have a higher risk of reactivation/reinfection than those infected with the gB1 genotype.
authors: Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
authors: Chang-Sheng
authors: CS
authors: Xia
authors: Xiao-Tao
authors: XT
authors: Zhao
authors: Yuan-Yuan
authors: YY
authors: Sun
authors: Zheng
authors: Z
authors: Zhang
mesh_terms: D000328:Adult; D044466:Asian People; D016000:Cluster Analysis; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus