title: Determination of three alkylphenol isomers in various water samples using a new HPLC method based on a duet stationary phase.
abstract: The level of three alkylphenols (APs) 4-nonylphenol, 4-octylphenol and 4-tert-octylphenol was monitored in various water samples using a new developed and validated HPLC method. The HPLC method employed a column with a mixed-mode stationary phase (C18/SCX) using a mobile phase of water to methanol = 15:85 (v/v) delivered at a flow rate of 1 mL/min at room temperature. Both diode array, DAD and fluorescence, FLD detectors were employed. The method is linear when APs concentration ranged from 0.025 to 0.5 μg/mL with a DAD detection at 279 nm and between 0.0008 and 0.1 μg/mL when the detection was made by FLD (excitation at 220 nm, emission at 315 nm). The limit of detection and limit of quantitation for alkylphenols were found to be 5 and 15 ng/mL, respectively (detection by DAD). The method was employed with good results for the determination of APs in the presence of polycyclic aromatic hydrocarbons in various water samples.
authors: Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Blvd Regina Elisabeta, 030018 Bucharest, Romania.
authors: Ioana
authors: I
authors: Cruceru
authors: Andreea
authors: A
authors: Florescu
authors: Irinel Adriana
authors: IA
authors: Badea
authors: Luminita
authors: L
authors: Vladescu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D010636:Phenols; D014874:Water Pollutants, Chemical; C080417:4-octylphenol; C041594:4-nonylphenol; C105260:4-tert-octylphenol