title: Small bowel transplantation in outbred rats.
abstract: OBJECTIVE
  To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats.
  
  
  METHODS
  Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft.
  
  
  RESULTS
  Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7(th) and 15(th) postoperative day and eight (22.2%) between 34(th) and 47(th) postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%).
  
  
  CONCLUSIONS
  Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.
authors: FMUSP, Sao Paulo, Brazil.
authors: Daniel Reis
authors: DR
authors: Waisberg
authors: André Dong Wong
authors: AD
authors: Lee
authors: Rafael Miyashiro Nunes dos
authors: RM
authors: Santos
authors: Eduardo Kenji
authors: EK
authors: Mory
authors: Anderson Lino
authors: AL
authors: Costa
authors: Edna Frasson de Souza
authors: EF
authors: Montero
authors: Eleazar
authors: E
authors: Chaib
authors: Luis Augusto Carneiro
authors: LA
authors: D'Albuquerque
authors: Flavio Henrique Ferreira
authors: FH
authors: Galvao
mesh_terms: D000208:Acute Disease; D000818:Animals; D006084:Graft Rejection / Q000401:mortality / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar / Q000145:classification; D012720:Severity of Illness Index; D013997:Time Factors