title: Scattered regulatory regions of the chicken immunoglobulin-β gene and two adjacent promoters of ubiquitously expressed genes interact with the immunoglobulin-β promoter in DT40 cells.
abstract: Recent studies indicate that several transcription units assemble to form a 'transcription factory' where active transcription occurs in the nuclei. Previously, we generated chicken B-lymphocyte-derived DT40 cells lacking six transcriptional regulatory regions scattered in and around the immunoglobulin (Ig)-β gene. The deletions caused a complete shut down of transcription and epigenetic regulation of the Ig-β gene, demonstrating that the scattered regulatory regions cooperated in the transcriptional and epigenetic regulation of the gene. However, the in vivo 3-dimensional spatial relationships between the Ig-β promoter and these six regulatory regions were not investigated. In this study, we used chromosome conformation capture (3C) technology and demonstrated that the Ig-β promoter physically interacted with the scattered regulatory regions. We found that the Ig-β promoter also interacted with two downstream promoters of ubiquitously expressed genes, rad motif 1 (RDM1) and Plekhm1, to form a transcription factory, but not with three ubiquitously expressed genes, BAF60b, p45/SUG, and RRMJ3, located upstream of the Ig-β gene. In this factory, the chromatin from the three promoters and the scattered regulatory regions of the Ig-β gene formed a complex structure with many chromatin loops.
authors: Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan.
authors: Tomohiro
authors: T
authors: Minbuta
authors: Masao
authors: M
authors: Ono
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D002843:Chromatin / Q000502:physiology; D002875:Chromosomes / Q000502:physiology; D044127:Epigenesis, Genetic; D005803:Genes, Immunoglobulin / Q000502:physiology*; D007136:Immunoglobulins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D002843:Chromatin; D007136:Immunoglobulins