title: Cigarette smoke exposure hinders long-term allograft survival by suppressing indoleamine 2, 3-dioxygenase expression.
abstract: Cigarette smoke causes cancer and increases the vulnerability of smokers to infections. Epidemiologic studies have shown that smoking is one of major risk factors for late allograft rejection. Despite statistical data that associate smoking with allograft rejection, no any study has been conducted to prove that cigarette smoke directly causes allograft rejection in a cause-effect manner. In particular, investigation into immunologic mechanisms underlying smoke-related allograft rejection is lacking. Here we found that second hand smoke (SHS) hindered long-term islet allograft survival induced by CD154 costimulatory blockade plus donor-specific splenocyte transfusion (DST), although it failed to alter acute islet allograft rejection. SHS did not directly interfere with vigorously alloreactive T-cell proliferation in vivo and in vitro. Neither naturally occurring nor induced CD4+CD25+ Treg cell numbers were significantly reduced by SHS. However, SHS suppressed mRNA and protein expression of indoleamine 2, 3-dioxygenase (IDO) and its activity upon transplantation while IDO overexpression in islet allografts restored their long-term survival induced by CD154 blockade. Therefore, SHS prevents long-term allograft survival by inhibiting IDO expression and activity. Thus, our study for the first time demonstrates that SHS shortens allograft survival in a cause-effect manner and unveils a novel immunologic mechanism underlying smoking-related allograft rejection.
authors: Department of Immunology and Microbiology, Center for Biomedical Research, University of Texas Health Science Center, Tyler, TX, USA.
authors: F
authors: F
authors: Wan
authors: H
authors: H
authors: Dai
authors: S
authors: S
authors: Zhang
authors: Y
authors: Y
authors: Moore
authors: N
authors: N
authors: Wan
authors: Z
authors: Z
authors: Dai
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006084:Graft Rejection / Q000209:etiology*; D006085:Graft Survival / Q000276:immunology*; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014184:Transplantation, Homologous
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D014028:Tobacco Smoke Pollution