title: Cloning and characterization of a novel tyrosine ammonia lyase-encoding gene involved in bagremycins biosynthesis in Streptomyces sp.
abstract: Tyrosine ammonia lyase catalyzes the deamination of L: -tyrosine to trans-coumaric acid. A novel tyrosine ammonia lyase-encoding gene, bagA, was cloned and sequenced from bagremycins-producing strain Streptomyces sp. Tü 4128 whose protein product contains a Ala-Ser-Gly segment in the active site. The disruption of the bagA gene abolished trans-coumaric acid and bagremycins production. trans-coumaric acid restored the formation of bagremycin A in the mutant, but not bagremycin B. Thus, trans-coumaric acid is a precursor for biosynthesis of bagremycins and the bagA gene codes for tyrosine ammonia lyase to synthesize trans-coumaric acid. This is a novel bacterial tal gene reported in actinomycetes for the second time and for the first time in a Streptomyces sp.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
authors: Yunxia
authors: Y
authors: Zhu
authors: Siyi
authors: S
authors: Liao
authors: Jiang
authors: J
authors: Ye
authors: Huizhan
authors: H
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D062365:Aminobenzoates / Q000378:metabolism; D000642:Ammonia-Lyases / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003373:Coumaric Acids / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014443:Tyrosine / Q000378:metabolism
chemical_list: D062365:Aminobenzoates; D003373:Coumaric Acids; D004269:DNA, Bacterial; C440631:bagremycin A; C440632:bagremycin B; D014443:Tyrosine; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase