title: Increased CXCL12 expression in the placentae of women with pre-eclampsia.
abstract: OBJECTIVE
  To investigate the expression pattern of CXC chemokine ligand-12 (CXCL12) in the placentae of normal and pre-eclamptic women.
  
  
  METHODS
  Twenty-five women with severe pre-eclampsia and 30 normotensive women, matched for gestational age, were enrolled in the study. Placental tissue from each woman was collected following delivery by caesarean section. Quantitative reverse transcription polymerase chain reaction, Western blot analysis and immunohistochemical staining were performed for mRNA expression, quantification and tissue localization of CXCL12 in each placenta.
  
  
  RESULTS
  CXCL12 expression was greater in pre-eclamptic placentae compared with normal placentae. CXCL12 was detected in most placental tissue cells by immunohistochemical staining. CXCL12 immunoreactivity was significantly greater in syncytiotrophoblasts of pre-eclamptic placentae compared with normal placentae. However, there was no significant difference in CXCL12 immunoreactivity in other tissues between the two groups.
  
  
  CONCLUSIONS
  CXCL12 expression is significantly greater in the placentae of pre-eclamptic women compared with normal women. This may represent part of a compensatory mechanism for pre-eclampsia.
authors: Department of Obstetrics and Gynaecology, Konkuk University Medical Centre, Konkuk University School of Medicine, Seoul, Republic of Korea.
authors: Han Sung
authors: HS
authors: Hwang
authors: Han Sung
authors: HS
authors: Kwon
authors: In Sook
authors: IS
authors: Sohn
authors: Yong Won
authors: YW
authors: Park
authors: Young Han
authors: YH
authors: Kim
mesh_terms: D000046:Academic Medical Centers; D000328:Adult; D015153:Blotting, Western; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D014327:Trophoblasts / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D011257:Pregnancy Proteins; D012333:RNA, Messenger