title: Micro/nanopatterning of proteins using a nanoimprint-based contact printing technique.
abstract: Micro and nanoscale protein patterning based on microcontact printing technique on large substrates have often resolution problems due to roof collapse of the poly(dimethylsiloxane) (PDMS) stamps used. Here, we describe a technique that overcomes these issues by using instead a stamp made of poly(methyl methacrylate) (PMMA), a much more rigid polymer that do not collapse even using stamps with very high aspect ratios (up to 300:1). Conformal contact between the stamp and the substrate is achieved because of the homogeneous pressure applied via the nanoimprint lithography instrument, and it has allowed us to print lines of protein 150 nm wide, at a 400 nm period. This technique, therefore, provides an excellent method for the direct printing of high-density submicrometer scale patterns, or, alternatively, micro/nanopatterns spaced at large distances.
authors: Nanobioengineering Laboratory, Institute for Bioengineering of Catalonia, Barcelona, Spain. emartinez@pcb.ub.es
authors: Elena
authors: E
authors: Martínez
authors: Mateu
authors: M
authors: Pla-Roca
authors: Josep
authors: J
authors: Samitier
mesh_terms: D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011506:Proteins; D019904:Polymethyl Methacrylate