title: Human serum albumin nanotubes with esterase activity.
abstract: A nanocylindrical wall structure was obtained by layer-by-layer (LbL) assembly of poly-L-arginine (PLA) and human serum albumin (HSA) and characterized by scanning electron microscopy (SEM), scanning force microscopy (SFM), and cryogenic transmission electron microscopy (cryo-TEM). SEM and SFM measurements of a lyophilized powder of (PLA/HSA)(3) nanotubes yielded images of round, chimney-like architectures with approximately 100 nm wall thickness. Cryo-TEM images of the hydrated sample revealed that the tube walls are composed of densely packed HSA molecules. Moreover, when small-angle X-ray scattering was used to characterize the individual PLA and HSA components in aqueous solutions, maximum diameters of approximately 28 nm and 8 nm were obtained, respectively. These values indicate the minimum thickness of wall layers consisting of PLA and HSA. It can also be concluded from SEM as well as from cryo-TEM images that the protein cylinders are considerably swollen in the presence of water. Furthermore, HSA retains esterase activity if assembled in nanotubes, as indicated by measurements of para-nitrophenyl acetate hydrolysis under semi-physiological conditions (pH 7.4, 22 °C). The enzyme activity parameters (Michaelis constant, K(m), and catalytic constant, k(cat)) were comparable to those of free HSA.
authors: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan. komatsu@kc.chuo-u.ac.jp
authors: Teruyuki
authors: T
authors: Komatsu
authors: Takaaki
authors: T
authors: Sato
authors: Christoph
authors: C
authors: Boettcher
mesh_terms: D004950:Esterases / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D012709:Serum Albumin; D004950:Esterases