title: Exiguobacterium aquaticum sp. nov., a member of the genus Exiguobacterium.
abstract: A Gram-positive, motile, short rod-shaped, orange pigmented bacterium, designated strain IMTB-3094(T), was isolated from a water sample collected from Tikkar Tal Lake, Haryana, and subjected to detailed polyphasic taxonomic analysis. Strain IMTB-3094(T) possessed most of the phenotypic and chemotaxonomic properties of the genus Exiguobacterium and, based on 16S rRNA gene sequence analysis, was assigned to this genus. Strain IMTB-3094(T) exhibited the highest 16S rRNA gene sequence similarity to Exiguobacterium mexicanum MTCC 7759(T) (99.5 %) followed by Exiguobacterium aurantiacum MTCC 6414(T) (99.1 %), Exiguobacterium aestuarii MTCC 7750(T) (98.0 %), Exiguobacterium profundum MTCC 10851(T) (98.0 %) and Exiguobacterium marinum MTCC 7751(T) (98.0 %). The G+C content of the genomic DNA of strain IMTB-3094(T) was 53.2 mol% and a DNA-DNA relatedness study confirmed that it represents a novel species. The major fatty acids of strain IMTB-3094(T) were iso-C(17 : 0) (16.1 %), anteiso-C(13 : 0) (19.0 %), iso-C(13 : 0) (11.9 %), iso-C(15 : 0) (9.8 %) and iso-C(17 : 1) (12.7 %). The predominant quinones were MK-7 (55.0 %) and MK-6 (26.0 %) with minor amounts of MK-8 (12.0 %). Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain IMTB-3094(T) represents a novel species of the genus Exiguobacterium, for which the name Exiguobacterium aquaticum sp. nov. is proposed. The type strain is IMTB-3094(T) (= MTCC 10958(T) = JCM 17977(T)).
authors: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh 160036, India.
authors: Revti
authors: R
authors: Raichand
authors: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh 160036, India.
authors: Siddhika
authors: S
authors: Pareek
authors: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh 160036, India.
authors: Nitin Kumar
authors: NK
authors: Singh
authors: Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh 160036, India.
authors: Shanmugam
authors: S
authors: Mayilraj
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D016987:Gram-Positive Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007194:India; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2