title: [Effects of interleukin-1β on mineralization potential of dental pulp stem cells].
abstract: OBJECTIVE
  To investigate the effects of pro-inflammatory cytokine interleukin-1β (IL-1β) on mineralization potential of dental pulp stem cells (DPSC).
  
  
  METHODS
  Rat DPSC were cultured in vitro and randomly divided into three groups, IL-1β (10 µg/L), osteogenic inductive medium and non-osteogenic inductive medium. After 3, 7, and 12 days of treatment, the cultures were evaluated for cell proliferation and calcium deposit. Real-time polymerase chain reaction was used to detect the gene expression levels of osteocalcin (OC), bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP-1). In vivo test, after 3 day's treatment with IL-1β, the cell-scaffold complexes were implanted subcutaneously in mice for 8 weeks. Histological analysis was performed to evaluate hard tissue formation.
  
  
  RESULTS
  In vitro test, after 3-day's treatment, IL-1β improved cell proliferation to 137.22 DNA µg/L and cell viability becomes (97.12 ± 7.18)% of control. The gene expression levels of OC, BSP, DSPP and DMP-1 are (378.19 ± 16.22)%, (427.12 ± 18.22)%, (247.19 ± 10.11)% and (198.29 ± 10.23)% respectively. The results of IL-1β's group was notable increased compared with non-osteogenic induction medium and the statistical differences are significant. IL-1β induced the odontogenic differentiation of DPSC. However, these effects tended to continuously decrease with treatment time. Histological analysis demonstrated that in the group treated with IL-1β hard tissue was markedly formed in vivo.
  
  
  CONCLUSIONS
  IL-1β may induce the mineralization of DPSC and play an important role in host defenses and tissue repair.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology, Wuhan University, Wuhan, China.
authors: Xue-chao
authors: XC
authors: Yang
authors: Si-yuan
authors: SY
authors: Zhang
authors: Ming-wen
authors: MW
authors: Fan
authors: Xin
authors: X
authors: Li
authors: Tian
authors: T
authors: Liu
authors: Yao
authors: Y
authors: Yao
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D053583:Interleukin-1beta; D015675:Osteocalcin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C081635:Dmp1 protein, rat