title: Reperfusion of the rat brain tissues following acute ischemia: the correlation among diffusion-weighted imaging, histopathology, and aquaporin-4 expression.
abstract: BACKGROUND
  Although some studies have reported that aquaporin-4 (AQP4) plays a role in the post-ischemic edema formation and diffusion-weighted imaging (DWI), little is known about the AQP4 expression in stage of the reperfusion following acute cerebral ischemia, as well as the correlation between histopathology and DWI. The aim of the study was to investigate the correlation among DWI, histopathology and the AQP4 expression in the reperfused rat brain tissues following acute ischemia.
  
  
  METHODS
  Seventy Wistar rats were randomly divided into a control group (group A), and several occluded and reperfusion groups. They had their middle cerebral artery unilaterally occluded (MCAO) for 30 minutes (group B) followed by 30 minutes (group D) or 60 minutes (group E) of reperfusion, or 60 minutes of MCAO (group C) followed by 30 minutes (group F), or 60 minutes (group G) of reperfusion (n = 10 for each group). All rats underwent DWI scanning. The relative apparent diffusion coefficient (rADC) value of each rat was calculated. All the rats were sacrificed and the cerebral ischemic tissues were examined for histopathology. Real-time fluro-quantitative polymerase chain reaction (RT-PCR) and Western-blotting were performed. The amount of AQP4 mRNA (Ex(-ΔΔCt)) and AQP4 protein (Q) was statistically analyzed. The correlation between rADC values and AQP4 mRNA expression was analyzed with the Pearson correlation test.
  
  
  RESULTS
  In all the reperfusion groups, the areas of hyper-intensity signal in DWI were decreased, and the rADC value increased and the AQP4 expression decreased significantly compared with the occluded group (t = 26.89, t = 18.26, P < 0.01). There was a negative correlation between AQP4 mRNA expression and rADC values (r = -0.72, P < 0.01). A mixed edema, composed of cerebral intracelluar edema and vasogenic brain edema, was observed in all the reperfusion groups. It was more prevalent in groups D and F than in the groups E and G. With the reperfusion time postponed, the cerebral intracelluar edema of the rat was significantly mitigated, but the vasogenic brain edema was not significantly changed.
  
  
  CONCLUSIONS
  There is a close correlation between AQP4 expression and the cerebral intracellular edema. The change of ADC values may indirectly reflect the level of the AQP4 expression. DWI may become a promising, noninvasive imaging modality to predict early stroke and reperfusion injury.
authors: Department of Radiology, Affiliated Haikou Hospital of Xiangya School of Medicine, Central South University (Haikou Municipal People's Hospital), Haikou, Hainan 570208, China. cqluh@sohu.com
authors: Hong
authors: H
authors: Lu
authors: Hui
authors: H
authors: Hu
authors: Zhan-Ping
authors: ZP
authors: He
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000032:analysis*; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000628:therapy*; D002560:Cerebrovascular Circulation; D038524:Diffusion Magnetic Resonance Imaging; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
chemical_list: D051401:Aquaporin 4