title: Image analysis of colocalization of nuclear DNA and GFP labelled HIF-1α in stable transformants.
abstract: HIF-1α is regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to examine the activity quantitatively. In the present study, we proposed a method where stable expression system of HIF-1α was combined with image correlation analysis. When the stable transformants were labeled with DRAQ5, we could detect Co2+-induced nuclear translocation by the use of cross-correlation analysis of the dual labeling images. In the case of high throughput screening for HIF-1α-targeted drug, we should use Pearson's correlation coefficient to judge nuclear translocation.
authors: Department of Systems Life Engineering, Maebashi Institute of Technology, 460-1 Kamisadori, Maebashi.
authors: Takehito
authors: T
authors: Goto
authors: Michihiko
authors: M
authors: Sato
authors: Eiji
authors: E
authors: Takahashi
authors: Yasutomo
authors: Y
authors: Nomura
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D003035:Cobalt / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal
chemical_list: C520537:1,5-bis((2-(methylamino)ethyl)amino)-4,8-dihydroxyanthracene-9,10-dione; D000880:Anthraquinones; D005456:Fluorescent Dyes; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D049452:Green Fluorescent Proteins; D003035:Cobalt; D004247:DNA; C018021:cobaltous chloride