title: Genome-wide identification,  phylogeny and expression analysis of the lipoxygenase gene family in cucumber.
abstract: Plant  lipoxygenase (LOX) is involved in growth and developmental control processes,  through the biosynthesis of regulatory molecules and defense responses to  pathogens, wounding and stress. To date, few LOX proteins and little tissue  expression profiling have been reported in detail for cucumber (Cucumis  sativus L.). Recent completion of the cucumber genome sequence now permits  genome-wide analysis of the LOX gene family in cucumber as well as comparison  with LOX in Arabidopsis and rice. We identified 23 candidate LOX genes  in the cucumber genome; phylogenetic analysis indicated that these LOX members  cluster into two groups, designated types 1 and 2, as expected from previous  studies. Sequence analysis showed that five binding sites of iron, including  two consensus histidines in the LOX domain, are highly conserved in the  cucumber LOX proteins. Analysis of chromosomal localization and genome  distribution suggested that tandem duplication and/or polyploidal duplication  contributed to the expansion of the cucumber LOX gene family. Based on  intron/exon structure analysis, only a few of the extant intron patterns  existed in the ancestor of monocots and eudicots. Expression data showed  widespread distribution of the cucumber LOX gene family within plant tissues,  suggesting that they perform different functions in different tissues.
authors: School of Sciences, Jiangxi Agricultural University, Nanchang, China. lsq_hn306@163.com
authors: S Q
authors: SQ
authors: Liu
authors: X H
authors: XH
authors: Liu
authors: L W
authors: LW
authors: Jiang
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D018553:Cucumis sativus / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018745:Genome, Plant / Q000502:physiology; D055106:Genome-Wide Association Study; D008084:Lipoxygenase / Q000096:biosynthesis / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D010940:Plant Proteins; D008084:Lipoxygenase