title: The hsa-miR-5787 represses cellular growth by targeting eukaryotic translation initiation factor 5 (eIF5) in fibroblasts.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate diverse biological processes. We cloned novel small RNA from human mesenchymal stem cells (hMSCs) and termed microRNA-5787 (hsa-miR-5787) that met the criteria for a miRNA. The level of miR-5787 was elevated in senescent fibroblasts. Based on the target prediction algorithm and results that were obtained, we find that eukaryotic translation initiation factor 5 (eIF5) is a target of miR-5787. Similar to the over-expression of miR-5787, we showed that repression of eIF5 in fibroblasts negatively affected cell growth. Therefore, we propose that the miR-5787 represses cell growth, in part, by targeting eIF5.
authors: Department of Pharmacy, College of Pharmacy, CHA University, 222 Yatap-Dong, Bundang-Gu, Seongnam-Si, Republic of Korea.
authors: Hanna
authors: H
authors: Yoo
authors: Jung Ki
authors: JK
authors: Yoo
authors: Jimin
authors: J
authors: Lee
authors: Dong Ryul
authors: DR
authors: Lee
authors: Jung Jae
authors: JJ
authors: Ko
authors: Seoung Hun
authors: SH
authors: Oh
authors: Young Kug
authors: YK
authors: Choo
authors: Jin Kyeoung
authors: JK
authors: Kim
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D015854:Up-Regulation; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D035683:MicroRNAs; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C569502:MIRN5787 microRNA, human