title: Sustained release of adiponectin improves osteogenesis around hydroxyapatite implants by suppressing osteoclast activity in ovariectomized rabbits.
abstract: Lack of estrogen could lead to decreased bone mass and increased risk for osteoporosis, which has a negative influence on biomaterial implantation. Adiponectin (APN), an adipose-derived hormone, has been shown to increase bone density by inhibiting osteoclast formation and promoting the formation of osteoblasts. This study was designed to investigate the direct effects of APN released from the Matrigel controlled-release system on the activity of rabbit mature osteoclasts and osteoclast precursor RAW264.7 cells in vitro, and to determine its effects by improving osteogenesis around the hydroxyapatite (HA) implant in ovariectomized (OVX) rabbits. APN+Matrigel+HA, APN+HA, Matrigel+HA and HA were implanted into mandibular defects of OVX rabbits. At 4 weeks after implantation, the mandibles were examined by histology, microcomputed tomography and biomechanical testing. The results demonstrated that Matrigel extended the length of APN released to achieve long-term persistence. The sustained release of APN suppressed the osteoclastic activity both in vitro and in vivo, and improved the peri-implant osteogenesis in OVX rabbits, while the short-term APN treatment did not. Sustained release of APN may be an effective strategy to improve the restoration of bone defects by the use of HA materials under osteoporotic conditions in which osteoclasts are highly activated.
authors: Division of Oral Biology, Department of General Dentistry, Tufts University School of Dental Medicine, Boston, MA 02111, USA.
authors: En
authors: E
authors: Luo
authors: Jing
authors: J
authors: Hu
authors: Chongyun
authors: C
authors: Bao
authors: Yunfeng
authors: Y
authors: Li
authors: Qisheng
authors: Q
authors: Tu
authors: Dana
authors: D
authors: Murray
authors: Jake
authors: J
authors: Chen
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001696:Biomechanical Phenomena / Q000187:drug effects; D001862:Bone Resorption / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D003692:Delayed-Action Preparations; D015536:Down-Regulation / Q000187:drug effects; D004338:Drug Combinations; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D006801:Humans; D020007:Implants, Experimental; D007527:Isoenzymes / Q000378:metabolism; D007797:Laminin / Q000737:chemistry; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D010052:Ovariectomy; D011509:Proteoglycans / Q000737:chemistry; D011817:Rabbits; D000071681:Tartrate-Resistant Acid Phosphatase; D055114:X-Ray Microtomography
chemical_list: D052242:Adiponectin; D003692:Delayed-Action Preparations; D004338:Drug Combinations; D007527:Isoenzymes; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D004247:DNA; D017886:Durapatite; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D020169:Caspases