title: Heme ligand identification and redox properties of the cytochrome c synthetase, CcmF.
abstract: Cytochrome c maturation in many bacteria, archaea, and plant mitochondria involves the integral membrane protein CcmF, which is thought to function as a cytochrome c synthetase by facilitating the final covalent attachment of heme to the apocytochrome c. We previously reported that the E. coli CcmF protein contains a b-type heme that is stably and stoichiometrically associated with the protein and is not the heme attached to apocytochrome c. Here, we show that mutation of either of two conserved transmembrane histidines (His261 or His491) impairs stoichiometric b-heme binding in CcmF and results in spectral perturbations in the remaining heme. Exogeneous imidazole is able to correct cytochrome c maturation for His261 and His491 substitutions with small side chains (Ala or Gly), suggesting that a "cavity" is formed in these CcmF mutants in which imidazole binds and acts as a functional ligand to the b-heme. The results of resonance Raman spectroscopy on wild-type CcmF are consistent with a hexacoordinate low-spin b-heme with at least one endogeneous axial His ligand. Analysis of purified recombinant CcmF proteins from diverse prokaryotes reveals that the b-heme in CcmF is widely conserved. We have also determined the reduction potential of the CcmF b-heme (E(m,7) = -147 mV). We discuss these results in the context of CcmF structure and functions as a heme reductase and cytochrome c synthetase.
authors: Department of Biology, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
authors: Brian
authors: B
authors: San Francisco
authors: Eric C
authors: EC
authors: Bretsnyder
authors: Kenton R
authors: KR
authors: Rodgers
authors: Robert G
authors: RG
authors: Kranz
mesh_terms: D019943:Amino Acid Substitution; D001665:Binding Sites; D055162:Biocatalysis; D004789:Enzyme Activation / Q000187:drug effects; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry; D020035:Holoenzymes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology; D008024:Ligands; D008190:Lyases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D029968:Escherichia coli Proteins; D020035:Holoenzymes; D007093:Imidazoles; D007202:Indicators and Reagents; D008024:Ligands; D050505:Mutant Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D006418:Heme; D006639:Histidine; C029899:imidazole; D008190:Lyases; C031581:cytochrome C synthetase