title: Characterisation of PSE zones in semimembranosus pig muscle.
abstract: Pig semimembranosus muscles, sampled from normal hams or from PSE-zones of defective hams, were analysed by histochemistry and electrophoretic techniques. PSE zones were characterised by a disorganisation of fibre alignment and a significant increase of inter fibre spacing (26.2% vs. 16.9%, p<0.05). Protein solubility was significantly lower in defective muscle (55.4 vs. 91.5mg/g, p<0.001). SDS-PAGE evidenced in such samples a lower abundance of the 97, 40 and 26kDa bands in the sarcoplasmic fraction and a higher abundance of the 97, 58, 34, 31, 15 and 11kDa bands in the myofibrillar fraction. Intensity of the MHC band (200kDa) was lower in PSE zone samples. By 2-D electrophoresis, it was shown that troponin T, MLC 1 and alpha-crystallin were less proteolysed in defective muscles, while creatine kinase fragments were more represented. One form of HSP 27 was absent from PSE zone samples. Overall, meat from PSE-zones and fast pH fall-PSE meat show numerous histological and biochemical similarities, particularly in their protein characteristics.
authors: Station de Recherches sur la Viande, INRA, 63122 St Genès-Champanelle, France.
authors: Elisabeth
authors: E
authors: Laville
authors: Thierry
authors: T
authors: Sayd
authors: Véronique
authors: V
authors: Santé-Lhoutellier
authors: Martine
authors: M
authors: Morzel
authors: Roland
authors: R
authors: Labas
authors: Michel
authors: M
authors: Franck
authors: Christophe
authors: C
authors: Chambon
authors: Gabriel
authors: G
authors: Monin