title: Transcriptomic responses to aluminum stress in soybean roots.
abstract: Aluminum (Al) toxicity is the primary limitation to crop production and plant growth in acid soils. Soybean has multiple mechanisms of Al resistance including the complexing and exclusion of Al in root apices by Al-induced citrate secretion. Microarray analysis is available for the identification of genes in soybean. In the present study, Affymetrix soybean genome array was used to identify the Al-induced differentially expressed genes in Al-resistant genotype Jiyu 70. With a cutoff of > 2.0-fold (p < 0.05) between non Al-treated and Al-treated root apices, 561 genes were upregulated and 78 genes were downregulated when roots were exposed to 30 μmol/L AlCl(3) for 4 h. Quantitative real-time PCR was used to test the microarray data. The analysis showed that nearly half of the Al-responsive genes were of unknown biological function. A higher proportion of genes related to transcription regulation and cell wall processes were observed in Al-induced up- and downregulated genes, respectively. Some genes homologous to the citrate transporter MATE family gene or C(2)H(2) family transcription factor gene, STOP1, were detected in our analysis. Some genes related to lignin deposition were upregulated, which might be related to Al-induced root elongation inhibition.
authors: Agriculture Ecology and Environment laboratory, College of Plant Science, Jilin University, Changchun 130062, PR China.
authors: Jiangfeng
authors: J
authors: You
authors: Hongmei
authors: H
authors: Zhang
authors: Ning
authors: N
authors: Liu
authors: Lingling
authors: L
authors: Gao
authors: Lingnan
authors: L
authors: Kong
authors: Zhenming
authors: Z
authors: Yang
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D002473:Cell Wall / Q000235:genetics / Q000502:physiology; D002712:Chlorides / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D010940:Plant Proteins; D014157:Transcription Factors; D000077410:Aluminum Chloride