title: Distribution of bone marrow-derived cells in the fracture callus during plate fixation in a green fluorescent protein-chimeric mouse model.
abstract: To clarify the distribution of bone-marrow-derived cells in fractures treated by plate fixation, fracture models were created using the green fluorescent protein (GFP) chimeric mouse. We observed 2 types of fracture healing processes with different types of callus formation and cellular events by using Mouse Fix™, a device allowing plate fixation on the mouse femur, and differences in the distribution of bone-marrow-derived cells between the 2 types. The GFP chimeric mice were created by bone marrow transplantation. Fractures were created on the left femurs of mice and stabilized with either rigid (Group R) or flexible (Group F) plates to prepare undecalcified fresh-frozen sections. In Group F, a large external callus and a large intramedullary callus were formed mostly by endochondral ossification. The cells that made up the intramedullary callus and callus in the fracture gap were GFP positive, but most cells of the external callus were not. In Group R, bone union was achieved mostly without external callus formation, bone apposition occurred directly in the gap, and a small intramedullary callus was formed. As observed in Group F, this group had GFP-positive cells in the callus within the fracture gap and in the intramedullary calluses. The results of this study provided direct evidence of the distribution of bone-marrow-derived cells in the callus of fractures treated by plate fixation under different stability conditions.
authors: Kitasato University School of Medicine, Department of Orthopaedic Surgery, 1–15–1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan .
authors: Masaki
authors: M
authors: Ueno
authors: Kentaro
authors: K
authors: Uchida
authors: Masashi
authors: M
authors: Takaso
authors: Hiroaki
authors: H
authors: Minehara
authors: Kaori
authors: K
authors: Suto
authors: Naonobu
authors: N
authors: Takahira
authors: Roland
authors: R
authors: Steck
authors: Michael A
authors: MA
authors: Schuetz
authors: Moritoshi
authors: M
authors: Itoman
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D001860:Bone Plates; D002146:Bony Callus / Q000166:cytology* / Q000502:physiology*; D002678:Chimera; D004195:Disease Models, Animal; D005269:Femur / Q000293:injuries* / Q000503:physiopathology*; D017102:Fracture Healing / Q000502:physiology*; D050723:Fractures, Bone / Q000503:physiopathology*; D049452:Green Fluorescent Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010012:Osteogenesis / Q000502:physiology*
chemical_list: D049452:Green Fluorescent Proteins