title: An effective way to biosynthesize α-glucosyl eugenol with a high yield by Xanthomonas maltophilia.
abstract: BACKGROUND
  Eugenol is known for its analgesic, local anesthetic, anti-inflammatory, antibacterial, and hair growing effects, the application of which, however, is limited by its low solubility, liability of sublimating, and its pungent smell. Compared to eugenol, its glycosylated derivate [eugenol α-glucoside (α-EG)] has more advantages in application.
  
  
  OBJECTIVE
  The biosynthesis of α-EG by Xanthomonas maltophilia Hugh (Xanthomonadaceae) BT-112 and the optimum conditions for α-EG production are investigated here.
  
  
  METHODS
  The α-EG was obtained by fermentation using Xanthomonas maltophilia BT-112 and purified by macroporous absorption resin. The identity of α-EG is confirmed by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR).
  
  
  RESULTS
  The maximum yield of α-EG reached 10.62 g/L broth when the suspension of Xanthomonas maltophilia strain was incubated at 30°C with 70 mM eugenol and 1.0 M maltose.
  
  
  CONCLUSIONS
  Bio-fermentation was applied in this work to get α-EG with a high mole conversion, which is a potentially efficient and highly promising approach to modify phenolic compounds into glucosides.
authors: College of Life Science & Technology, Beijing University of Chemical Technology, Beijing, China.
authors: Chang
authors: C
authors: Chen
authors: Min
authors: M
authors: Xiao
authors: Lingling
authors: L
authors: Deng
authors: Lihui
authors: L
authors: Yuan
authors: Peng
authors: P
authors: Zhang
mesh_terms: D000779:Anesthetics, Local / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055162:Biocatalysis; D002626:Chemistry, Pharmaceutical; D005054:Eugenol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008320:Maltose / Q000378:metabolism; D009812:Odorants; D012995:Solubility; D020615:Stenotrophomonas maltophilia / Q000254:growth & development / Q000378:metabolism*; D013237:Stereoisomerism; D014835:Volatilization
chemical_list: D000779:Anesthetics, Local; D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005960:Glucosides; D005054:Eugenol; D008320:Maltose