title: Survival time of cardiac allografts prolonged by isogeneic BMT in mice.
abstract: To find an approach to prolong the survival time of cardiac allografts in a BALB/c-to-C57/BL6 heterotopic heart transplant model and to try to figure out related chemokines and cytokines, isogeneic and allogeneic BM cells were obtained from pregnant C57/BL6 (♀C57/BL6 × ♂BALB/c) and regular BALB/c mice and injected to the half lethally irradiated C57/BL6 mice 1 day before heart transplantation. Recipients were treated with CsA or phosphate-buffered saline for 7 days. Isogeneic BMT (iBMT) from pregnant C57/BL6 mice was observed to significantly prolong the survival of BALB/c allografts and reduce the lymphocyte infiltration. Allogeneic BMT (aBMT) and iBMT both exhibited signicantly less T-cell proliferation reactivity and the similar degree of chimerism. There was no significant difference in these groups of IFN-γ and IL-4 production. The level of chemokine MIG (CXCL9) dramatically decreased in aBMT and iBMT groups compared with the control group. But there were no significant differences between aBMT and iBMT group. IL-17 and RORγ(t) (receptor-related orphan receptor) production were downregulated in iBMT recipients. These results indicate that iBMT can prolong the survival of cardiac allografts. IL-17 production downregulated in iBMT recipients. This means that iBMT may have important therapeutic implications.
authors: Department of Kidney Disease Center, The First Affiliated Hospital College of Medicine, Zhejiang University, Qinchun Road #79, Hangzhou, Zhejiang, China.
authors: Z
authors: Z
authors: Chen
authors: H
authors: H
authors: Jiang
authors: R
authors: R
authors: Chen
authors: S
authors: S
authors: Feng
authors: J
authors: J
authors: Jin
authors: Y
authors: Y
authors: Bi
authors: H
authors: H
authors: Yang
authors: J
authors: J
authors: Chen
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D016572:Cyclosporine / Q000494:pharmacology; D016207:Cytokines / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000517:prevention & control*; D006085:Graft Survival; D016027:Heart Transplantation; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
chemical_list: D016207:Cytokines; D007166:Immunosuppressive Agents; D016572:Cyclosporine