title: Core/shell Fe3O4 @SiO2 nanoparticles modified with PAH as a vector for EGFP plasmid DNA delivery into HeLa cells.
abstract: Novel stable core/shell Fe(3)O(4)@SiO(2)/PAH nanoparticles are synthesized using 15 nm Fe(3)O(4) as the template that is modified with PAH. The resulting nanoparticles can absorb plasmid DNA to mediate gene transfer in cultured HeLa cells. An electrophoretic assay suggests that the Fe(3)O(4)@SiO(2)/PAH nanoparticles protect the plasmid DNA from serum and DNase I degradation. A cell viability assay shows that the Fe(3)O(4)@SiO(2)/PAH nanoparticles exhibit a low cytotoxicity toward endothelial cells. Qualitative analysis of transfection in HeLa cells by nanoparticles carrying a plasmid DNA encoding EGFP demonstrates a fairly high expression level, even in the presence of serum. Thus, Fe(3)O(4)@SiO(2)/PAH nanoparticles are biocompatible and suitable for nonviral delivery, and may find applications in cancer therapy.
authors: Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
authors: Mengran
authors: M
authors: Shi
authors: Yiyao
authors: Y
authors: Liu
authors: Mingming
authors: M
authors: Xu
authors: Hong
authors: H
authors: Yang
authors: Chunhui
authors: C
authors: Wu
authors: Hirokazu
authors: H
authors: Miyoshi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D014162:Transfection / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000628:therapy*
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D011073:Polyamines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C063994:polyallylamine; D012822:Silicon Dioxide; D004247:DNA; D003850:Deoxyribonuclease I; D052203:Ferrosoferric Oxide