title: Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions.
abstract: The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell migration, and results in significant increases in cell growth of undifferentiated cells. These chemically defined xeno-free substrates generate more than three times the number of cells than feeder-containing substrates per surface area. Further, reprogramming and typical gene-targeting protocols can be readily performed on these engineered surfaces. These substrates provide an attractive cell culture platform for the production of clinically relevant factor-free reprogrammed cells from patient tissue samples and facilitate the definition of standardized scale-up friendly methods for disease modeling and cell therapeutic applications.
authors: The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors: Krishanu
authors: K
authors: Saha
authors: Ying
authors: Y
authors: Mei
authors: Colin M
authors: CM
authors: Reisterer
authors: Neena Kenton
authors: NK
authors: Pyzocha
authors: Jing
authors: J
authors: Yang
authors: Julien
authors: J
authors: Muffat
authors: Martyn C
authors: MC
authors: Davies
authors: Morgan R
authors: MR
authors: Alexander
authors: Robert
authors: R
authors: Langer
authors: Daniel G
authors: DG
authors: Anderson
authors: Rudolf
authors: R
authors: Jaenisch
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods; D010126:Ozone / Q000737:chemistry; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D019076:Transgenes; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011137:Polystyrenes; D010126:Ozone