title: Ampicillin Synthesis Using a Two-Enzyme Cascade with Both α-Amino Ester Hydrolase and Penicillin G Acylase.
abstract: The current enzymatic production of semisynthetic β-lactam antibiotics requires isolation and purification of the intermediate 6-aminopenicillanic acid which adds cost and complexity to the manufacturing process. In this work, we took advantage of the unique substrate specificity of a-amino ester hydrolases to perform a purely aqueous one-pot production of ampicillin from penicillin G and D-phenylglycine methyl ester, catalyzed by α-amino ester hydrolase and penicillin G acylase. The synthesis was performed in both a one-pot, one-step synthesis resulting in a maximum conversion of 39%, and a one-pot, two-step process resulting in a maximum conversion of 47%. The two-enzyme cascade reported in this paper is a promising alternative to the current enzymatic two-step, two-pot manufacturing process for semisynthetic β-lactam antibiotics which requires intermittent isolation of 6-aminopenicillanic acid.
authors: School of Chemical and Biomolecular Engineering Parker H. Petit Institute of Bioengineering and Bioscience 315 Ferst Drive, Atlanta, GA 30332-0363 (USA).
authors: Janna K
authors: JK
authors: Blum
authors: Andria L
authors: AL
authors: Deaguero
authors: Carolina V
authors: CV
authors: Perez
authors: Andreas S
authors: AS
authors: Bommarius