title: In vitro cultivation of axenic amastigotes and the comparison of antioxidant enzymes at different stages of Leishmania tropica.
abstract: The present study aimed to establish a simple method to yield large amounts of   Leishmania tropica amastigote-like forms in axenic cultures and to compare the superoxide   dismutase (SOD) and glutathione peroxidase (GPX) enzymes at different stages of L. tropica.   Different culture conditions were tested to find the optimum condition of axenic amastigotes   generation. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were   determined at logarithmic and stationary phases and axenic amastigote stage of the parasite.   A high proportion (88%) of amastigote-like forms of L. tropica was observed in BHI medium   supplemented with 20% FCS, pH 4.5, and incubated at 37ºC with 5% CO(2). The results showed   that SOD activity was at the lowest level in the logarithmic phase of promastigotes and   increased towards the stationary phase of promastigotes and amastigote stage. The results   showed that the optimum condition for differentiation of L. tropica promastigotes to axenic   amastigotes was BHI medium containing 20% FCS at pH 4.5, incubated at 37ºC in the presence   of 5% CO(2). It seems that SOD, but not GPX is a major determinant of intracellular survival of   the parasite.
authors: Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran. bahrami.somayeh@gmail.com
authors: S
authors: S
authors: Bahrami
authors: G R
authors: GR
authors: Hatam
authors: M
authors: M
authors: Razavi
authors: S
authors: S
authors: Nazifi
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007895:Leishmania tropica / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D010274:Parasitology / Q000379:methods*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase