title: [Calcitonin gene-related peptide induces phenotypic transformation of corpus cavernosum smooth muscle cells in diabetic rats with erectile dysfunction].
abstract: OBJECTIVE
  To explore the effect of the calcitonin gene related peptide (CGRP) on the phenotypic transformation of corpus cavernosum smooth muscle cells (CCSM) in diabetic rats with erectile dysfunction (ED).
  
  
  METHODS
  Models of diabetes and diabetic ED were established in male Sprague-Dawley rats by administration of streptozotocin, and CCSMs were primarily cultured and subjected to immunocytochemical assay. The cells were divided into a diabetic ED and a normal control group, and exposed to 0, 10, 60 and 100 nmol/L of CGRP for 24 hours. Then the relative expressions of calponin 1 (Cnn1) and osteopontin (OPN) mRNA were determined by real-time fluorescence quantitative RT-PCR (qRT-PCR).
  
  
  RESULTS
  The rate of SMalpha-actin positive cells in the CCSMs was (95.94 +/- 0.03) %. The expression of Cnn1 mRNA was significantly lower while that of OPN mRNA remarkably higher in the diabetic ED rats (4.41 +/- 0.29 and 5.28 +/- 0.32) than in the normal controls (10.35 +/- 0.62 and 1.32 +/- 0.24) (P < 0.01). Exposure to 100 nmol/L of CGRP significantly upregulated the expression of Cnn1 mRNA and downregulated that of OPN mRNA as compared with the unexposed rats (6.9 +/- 0.22 vs 4.41 +/- 0.29 and 3.26 +/- 0.31 vs 5.28 +/- 0.32, P < 0.01).
  
  
  CONCLUSIONS
  CGRP can transform the phenotype of CCSMs in diabetic ED rats from contractile to synthetic type.
authors: Department of Urology, Medical Center for Overseas Patients, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors: Shu-hua
authors: SH
authors: He
authors: An-yang
authors: AY
authors: Wei
authors: Ting-yu
authors: TY
authors: Ye
authors: Yong
authors: Y
authors: Yang
authors: Xin-gui
authors: XG
authors: Luo
authors: Yang
authors: Y
authors: Liu
authors: Tao
authors: T
authors: Zhang
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D007172:Erectile Dysfunction / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D010413:Penis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D000096985:Calponins
chemical_list: D015740:Calcitonin Gene-Related Peptide; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D053495:Osteopontin; D000096985:Calponins; C506897:Spp1 protein, rat