title: Cellular fusion and whitening effect of a chitosan derivative coated liposome.
abstract: In this study, a derivative of chitosan, N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC), was coated onto the liposomes made of cholesterol and 1,2-palmitoyl-sn-glycero-3-phosphatidylcholine (DPPC). These coated liposomes were loaded with kojic acid for skin whitening. The appearance of liposome was examined using transmission electron microscope (TEM), and the coating of HTCC to the liposome was confirmed by infrared spectroscopy. By labeling with Dil, the fusion of liposome with the cell membrane of L929 fibroblast and B16-F10 melanoma was improved by the coating of HTCC. Based on the results of Franz cell experiment, the penetration of kojic acid (KA) through skin was improved by using HTCC-coating liposomes. Furthermore, the cell proliferation of L929 was not affected by HTCC-coating liposomes, while that of B16-F10 was reduced slightly with the increase of the concentration of HTCC-loading liposome. The degree of skin whitening was determined based on the melanin content in B-16-F10 cells. The results showed that the level of melanin synthesis was lower when KA was delivered using HTCC-coating liposome instead of traditional liposome.
authors: Graduate Institute of Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors: Yang-Wei
authors: YW
authors: Wang
authors: Chi-Hsiung
authors: CH
authors: Jou
authors: Chia-Chun
authors: CC
authors: Hung
authors: Ming-Chien
authors: MC
authors: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002459:Cell Fusion; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis* / Q000378:metabolism; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008546:Melanoma, Experimental; D051379:Mice; D058990:Molecular Targeted Therapy / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013471:Sunburn / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013499:Surface Properties; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D008081:Liposomes; D008543:Melanins; C487756:N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride; D010713:Phosphatidylcholines; D011753:Pyrones; D000644:Quaternary Ammonium Compounds; D002686:Chitin; C011890:kojic acid; D002784:Cholesterol