title: Origin of Epilachnapaenulata defensive alkaloids: incorporation of [1-13C]-sodium acetate and [methyl-2H3]-stearic acid.
abstract: Ladybird beetles produce a large number of defensive alkaloids. Previous studies suggest that the structural diversity of these endogenous alkaloids can be traced to a common biosynthetic route based on the condensation of several acetate units. In this study, adults of Epilachna paenulata, a phytophagous neotropical species, were fed on diet enriched with potential precursors (sodium acetate, fatty acids and the amino acids lysine and ornithine) labeled with stable isotopes ((13)C, (2)H and (15)N). Labeled acetate was incorporated into the structurally related homotropane and piperidine alkaloids. The later also showed incorporation of [methyl-(2)H3] stearic acid. Our results hence support a fatty acid pathway for the biosynthesis of E. paenulata alkaloids. To our knowledge, this is the first report on the incorporation of a labeled fatty acid into a defensive piperidine alkaloid in insects.
authors: Laboratorio de Ecología Química, Facultad de Química, Universidad de la República, Avenida General Flores 2124, Montevideo CP 11800, Uruguay. scamaran@fq.edu.uy
authors: S
authors: S
authors: Camarano
authors: A
authors: A
authors: González
authors: C
authors: C
authors: Rossini
mesh_terms: D000470:Alkaloids / Q000096:biosynthesis*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes / Q000378:metabolism; D001517:Coleoptera / Q000378:metabolism*; D003903:Deuterium / Q000378:metabolism; D010880:Piperidines / Q000378:metabolism*; D019346:Sodium Acetate / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D000596:Amino Acids; D002247:Carbon Isotopes; D010880:Piperidines; D013229:Stearic Acids; D019346:Sodium Acetate; D003903:Deuterium