title: Bone substitution materials on the basis of BONITmatrix® up-regulate mRNA expression of IGF1 and Col1a1.
abstract: The aim of this study was to investigate the effects of BONITmatrix(®) and OSSA NOVA on the expression of growth factors and osteogenic differentiation. For this purpose, the mRNA expression of VEGF, IGF1, IGF2, collagen-1, collagen-2 and MMP8 was analysed in surgically created defects on the crania of adult male rats. Cranial samples were collected after implantation of BONITmatrix(®) or OSSA NOVA scaffolds for 4 weeks and determinations of gene expression were performed by quantitative RT-PCR. Real-time RT-PCR analyses showed a significantly higher expression of IGF1 in both groups treated with BONITmatrix(®) and OSSA NOVA compared to untreated controls, whereas type I collagen mRNA expression only increased in BONITmatrix(®) treated rats compared to controls. No changes in transcript expression of IGF2, VEGF, collagen-2 and MMP8 were detectable between the analysed groups. In conclusion, BONITmatrix(®) and OSSA NOVA stimulate the expression of growth factor IGF1, but only the granular dosage form is able to stimulate osteoblast differentiation.
authors: Department of Orthodontics, Preventive and Pediatric Dentistry, Ernst Moritz Arndt University of Greifswald, Germany. thomasgredes@yahoo.de
authors: Tomasz
authors: T
authors: Gredes
authors: Friedhelm
authors: F
authors: Heinemann
authors: Marzena
authors: M
authors: Dominiak
authors: Heike
authors: H
authors: Mack
authors: Tomasz
authors: T
authors: Gedrange
authors: Alexander
authors: A
authors: Spassov
authors: Thomas
authors: T
authors: Klinke
authors: Christiane
authors: C
authors: Kunert-Keil
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D024042:Collagen Type I / Q000096:biosynthesis*; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II / Q000096:biosynthesis; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000096:biosynthesis; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000494:pharmacology*; D054457:Tissue Scaffolds; D015854:Up-Regulation / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C519914:BONITmatrix; D001672:Biocompatible Materials; D018786:Bone Substitutes; C511513:COL2A1 protein, rat; D002130:Calcium Phosphates; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D012822:Silicon Dioxide; D020784:Matrix Metalloproteinase 8