title: Scanning electron microscopic evaluation of the successful sterilization of Lucilia cuprina (Wiedemann) utilized in maggot debridement therapy (mdt).
abstract: In Malaysia, maggot debridement therapy (MDT) utilizes maggots of Lucilia   cuprina (Wiedemann) to debride necrotic tissue from wound surface, reduce bacterial infection   and therefore, enhance wound healing process. To evaluate the sterility of the sterile maggots   produced after sterilization process before delivering onto patient wounds. Sterility of sterile   maggots is crucial in ensuring the safe usage of MDT and patient's health. Eggs of L. cuprina   collected from a laboratory colony were divided into treated group (sterilized) and control   group (non-sterilized). Treated group underwent sterilization while eggs from control group   were allowed to hatch without sterilization. Sodium hypochlorite and formaldehyde were the   main disinfectants used in this sterilization process. Scanning electron microscope (SEM)   was used to examine and ascertain the sterility of sterile maggots. SEM results showed that   all sterilized L. cuprina eggs and maggots achieved sterility and all were cleared from   bacterial contamination. In contrast, all non-sterilized eggs and maggots were found to be   colonized by microorganisms. Sterilization method employed to sterilize eggs and maggots   used in Malaysia MDT was proven successful and MDT is safe to be used as wound management   tools.
authors: Medical Entomology Unit, Infectious Diseases Research Centre (IDRC), Institute for Medical Research, Kuala Lumpur, Malaysia.
authors: Y S
authors: YS
authors: Yeong
authors: W A
authors: WA
authors: Nazni
authors: R L
authors: RL
authors: Santana
authors: I
authors: I
authors: Mohd Noor
authors: H L
authors: HL
authors: Lee
authors: A
authors: A
authors: Mohd Sofian
mesh_terms: D000818:Animals; D003646:Debridement / Q000379:methods; D004175:Diptera / Q000502:physiology* / Q000648:ultrastructure*; D005557:Formaldehyde / Q000633:toxicity; D006801:Humans; D007246:Infertility; D007814:Larva / Q000502:physiology / Q000648:ultrastructure; D008296:Malaysia; D008855:Microscopy, Electron, Scanning; D012973:Sodium Hypochlorite / Q000633:toxicity; D013245:Sterilization, Reproductive / Q000379:methods; D015053:Zygote / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D005557:Formaldehyde; D012973:Sodium Hypochlorite