title: Performance of peripheral (serum and molecular) blood markers for diagnosis of endometriosis.
abstract: OBJECTIVE
  To quantify the mRNA levels of MMP-3, MMP-9, VEGF and Survivin in peripheral blood and the serum levels of CA-125 and Ca19-9 in women with and without endometriosis and to investigate the performance of these markers to differentiate between deep and ovarian endometriosis.
  
  
  METHODS
  A case control study enrolled a series of 60 patients. Twenty controls have been matched with 20 cases of ovarian and 20 cases of deep endometriosis. Univariable and multivariable performance of serum CA125 and CA19-9, mRNA for Survivin, MMP9, MMP3 and VEGF genes have been evaluated by means of ROC curves and logistic regression, respectively.
  
  
  RESULTS
  No difference in markers' concentration was detected between ovarian and deep endometriosis. In comparison with controls, serum CA125 and CA19 yielded the better sensitivity followed by mRNA for Survivin gene (81.5, 51.9 and 7.5% at 10% false positive rate, respectively). Multivariable estimated odds of endometriosis yielded a sensitivity of 87% at the same false positive rate.
  
  
  CONCLUSIONS
  A combination of serum and molecular markers could allow a better diagnosis of endometriosis.
authors: Reproductive Medicine Unit, S. Orsola-Malpighi Hospital, via Massarenti 9, 40138 Bologna, Italy.
authors: Mohamed
authors: M
authors: Mabrouk
authors: Amira
authors: A
authors: Elmakky
authors: Elisabetta
authors: E
authors: Caramelli
authors: Antonio
authors: A
authors: Farina
authors: Giuseppe
authors: G
authors: Mignemi
authors: Stefano
authors: S
authors: Venturoli
authors: Gioia
authors: G
authors: Villa
authors: Manuela
authors: M
authors: Guerrini
authors: Linda
authors: L
authors: Manuzzi
authors: Giulia
authors: G
authors: Montanari
authors: Paola
authors: P
authors: De Sanctis
authors: Luisa
authors: L
authors: Valvassori
authors: Cinzia
authors: C
authors: Zucchini
authors: Renato
authors: R
authors: Seracchioli
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D018395:CA-19-9 Antigen / Q000097:blood; D016022:Case-Control Studies; D004715:Endometriosis / Q000097:blood* / Q000175:diagnosis; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000097:blood; D019278:Matrix Metalloproteinase 3 / Q000097:blood; D020780:Matrix Metalloproteinase 9 / Q000097:blood; D012372:ROC Curve; D000077022:Survivin; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: C107489:BIRC5 protein, human; D015415:Biomarkers; D018394:CA-125 Antigen; D018395:CA-19-9 Antigen; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D042461:Vascular Endothelial Growth Factor A; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9