title: Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolais bulls divergently selected for muscle growth.
abstract: This work aimed to investigate the consequences of muscle growth selection on muscle characteristics. An oxidative muscle (Rectus abdominis, RA) and a glycolytic one (Semitendinosus, ST) were studied in two groups of six extreme young Charolais bulls of high or low muscle growth. Mitochondrial activity was lower in muscles of bulls with high muscle growth. Transcriptomic studies allowed the identification of putatively differentially expressed genes. The differential expression between genetic types of two genes in RA (a heat shock protein and a thyroid receptor interacting protein) and of seven genes in ST (including LEU5, tropomyosin 2, and sarcosin) was confirmed by different statistical approaches or Northern blot analysis, as well as the differential expression of five genes (including PSMD4 and DPM synthase) between RA and ST. Both biochemical and transcriptomic results indicate that selection on muscle growth potential is associated with reduced slow-oxidative muscle characteristics. Further studies are required to understand the physiological importance of genes whose expression is changed by selection.
authors: INRA, Unité de Recherches sur les Herbivores, Theix, 63122 St-Genès-Champanelle, France.
authors: Karine
authors: K
authors: Sudre
authors: Isabelle
authors: I
authors: Cassar-Malek
authors: Anne
authors: A
authors: Listrat
authors: Yasuko
authors: Y
authors: Ueda
authors: Christine
authors: C
authors: Leroux
authors: Catherine
authors: C
authors: Jurie
authors: Charles
authors: C
authors: Auffray
authors: Gilles
authors: G
authors: Renand
authors: Patrice
authors: P
authors: Martin
authors: Jean-François
authors: JF
authors: Hocquette