title: Peripheral regulation by ecdysteroids of olfactory responsiveness in male Egyptian cotton leaf worms, Spodoptera littoralis.
abstract: Physiological and behavioral plasticity allows animals to adapt to changes in external (environmental) and internal (physiological) factors. In insects, the physiological state modulates adult behavior in response to different odorant stimuli. Hormones have the potential to play a major role in the plasticity of the olfactory responses. To explore if peripheral olfactory processing could be regulated by steroid hormones, we characterized the molecular, electrophysiological, and behavioral response to changes in endogenous hormone levels in adult male Spodoptera littoralis. The expression of the receptor complex (EcR/USP) was localized by in situ hybridization in the olfactory sensilla of antennae. Injections of 20-hydroxyecdysone (20E) induced an ecdysteroid signaling pathway in antennae and increased expression of the nuclear receptors EcR, USP and E75. Diacylglycerol kinase (DGK) and CaM expression were also up-regulated by 20E. Taken together, these molecular, electrophysiological, and behavioral results suggest a hormonal regulation of the peripheral olfactory processing in S. littoralis.
authors: UMR 1272 Physiologie de l'Insecte, Signalisation et Communication, Université Pierre et Marie Curie, Paris VI, 7 Quai Saint Bernard, F-75005 Paris, France.
authors: Laetitia
authors: L
authors: Bigot
authors: Haq Abdul
authors: HA
authors: Shaik
authors: Françoise
authors: F
authors: Bozzolan
authors: Virginie
authors: V
authors: Party
authors: Philippe
authors: P
authors: Lucas
authors: Stéphane
authors: S
authors: Debernard
authors: David
authors: D
authors: Siaussat
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D019852:Diacylglycerol Kinase / Q000378:metabolism; D026461:Ecdysteroids / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D010675:Pheromones; D011987:Receptors, Steroid / Q000378:metabolism; D015398:Signal Transduction; D012903:Smell / Q000502:physiology*; D018411:Spodoptera / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D004268:DNA-Binding Proteins; C087173:E75 protein, insect; D026461:Ecdysteroids; D019476:Insect Proteins; D010675:Pheromones; D011987:Receptors, Steroid; C055469:ecdysone receptor; D019852:Diacylglycerol Kinase