title: Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans.
abstract: Gluconobacter oxydans enable to oxidize sugars and polyols incompletely to corresponding materials with potential industrial applications, containing around 75 putative dehydrogenases. One of these putative dehydrogenases, Gox2181, was cloned and expressed in Escherichia coli BL21 (DE3), and its X-ray crystal structure was determined to a resolution of 1.8 Å. Gox2181 formed a homo-tetramer in the crystal that was coincident with the apparent molecular mass determined in the solution. Gox2181 displayed α/β-folding patterns, the conserved catalytic tetrad of Asn119-Ser147-Tyr162-Lys166, and the NAD-binding pocket, which aligned well with the 'classical' type of short-chain dehydrogenase/reductase (SDR) enzymes. Gox2181 was denoted SDR51C based on the SDR nomenclature system. The purified recombinant Gox2181 was demonstrated to be NAD(H)-dependent and active towards a wide range of substrates, including sugar alcohols, secondary alcohols, ketones, and ketoses. Among the substrates tested, Gox2181 displayed preference for secondary hydroxyl or carbonyl groups, showing low K(m) values with d-arabitol and butanedione.
authors: State Key Laboratory of Bioreactor Engineering, NewWorld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
authors: Xu
authors: X
authors: Liu
authors: Zuanning
authors: Z
authors: Yuan
authors: Y
authors: Y
authors: Adam Yuan
authors: Jinping
authors: J
authors: Lin
authors: Dongzhi
authors: D
authors: Wei
mesh_terms: D002384:Catalysis; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D020600:Gluconobacter oxydans / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry* / Q000235:genetics; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D044925:Oxidoreductases Acting on CH-CH Group Donors