title: Mesorhizobium silamurunense sp. nov., isolated from root nodules of Astragalus species.
abstract: Four rhizobial strains representing a previously defined novel group in the genus Mesorhizobium and isolated from Astragalus species in China were further characterized using a polyphasic approach. Phylogenetic analysis of 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus Mesorhizobium, with Mesorhizobium plurifarium LMG 11892(T) as the closest neighbour sharing a sequence similarity of 99.8 %. Comparative sequence analysis of the atpD, recA, glnII, rpoB, nodC and nifH genes, SDS-PAGE of whole-cell soluble proteins, DNA-DNA hybridization, fatty acid profiles and a series of phenotypic and physiological tests differentiated the novel group from all recognized species of the genus Mesorhizobium. Based on the data obtained in the present and previous studies, this group represents a novel species within the genus Mesorhizobium, for which the name Mesorhizobium silamurunense sp. nov. is proposed. The type strain is CCBAU 01550(T) (= HAMBI 3029(T) = LMG 24822(T)), and could form effective nodules on Astragalus membranaceus, Astragalus adsurgens and Caragana intermedia, and ineffective nodules on Phaseolus vulgaris in cross-nodulation tests.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.|State Key Laboratories for AgroBiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Chun Tian
authors: CT
authors: Zhao
authors: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF 11340, México.
authors: En Tao
authors: ET
authors: Wang
authors: State Key Laboratories for AgroBiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Yan Ming
authors: YM
authors: Zhang
authors: State Key Laboratories for AgroBiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Wen Feng
authors: WF
authors: Chen
authors: State Key Laboratories for AgroBiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Xin Hua
authors: XH
authors: Sui
authors: State Key Laboratories for AgroBiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors: Wen Xin
authors: WX
authors: Chen
authors: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Hong Can
authors: HC
authors: Liu
authors: Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors: Xiao Xia
authors: XX
authors: Zhang
mesh_terms: D027883:Astragalus Plant / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D059128:Mesorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D055170:Plant Root Nodulation; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S