title: Preparative isolation and purification of senkyunolide-I, senkyunolide-H and ferulic acid from Rhizoma Chuanxiong using counter-current chromatography.
abstract: Three active compounds, senkyunolide-I, senkyunolide-H and ferulic acid (FA), were successfully isolated and purified from the extracts of Rhizoma Chuanxiong by counter-current chromatography (CCC). Based on the principle of the partition coefficient values (k) for target compounds and the separation factor (α) between target compounds, the two-phase solvent system that contains n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 3:7:4:6 v/v was selected for the CCC separation, and the lower phase was employed as the mobile phase in the head-to-tail elution mode. In a single run, 400 mg of the crude extract yielded pure senkyunolide-I (6.4 mg), senkyunolide-H (1.7 mg) and FA (4.4 mg) with the purities of 98, 93 and 99%, respectively. The CCC fractions were analyzed by high-performance liquid chromatography, and the structures of the three active compounds were identified by MS and (1)H NMR.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Chaoyang District, Beijing, P. R. China. weiyun@mail.buct.edu.cn
authors: Yun
authors: Y
authors: Wei
authors: Jia
authors: J
authors: Hu
authors: Hao
authors: H
authors: Li
authors: Jiangang
authors: J
authors: Liu
mesh_terms: D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D003373:Coumaric Acids / Q000032:analysis / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D030004:Ligusticum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C045855:3-N-butyl-4,5-dihydrophthalide; C004999:ferulic acid