title: Antiplatelet activity of 3-butyl-6-bromo-1(3H)-isobenzofuranone on rat platelet aggregation.
abstract: This study aimed to explore the impact of 3-butyl-6-bromo-1(3H)-isobenzofuranone (Br-NBP) on rat platelet aggregation induced by arachidonic acid (AA). Anti-platelet activities in vitro and ex vivo in rat platelets and the possible mechanism were also investigated. The 50% inhibitory concentration (IC(50)) of Br-NBP was 84 μM in washed platelet added AA (final concentration, 780 μM) in vitro, meanwhile intravenous injection of Br-NBP also potently inhibited platelet aggregation ex vivo. Br-NBP significantly restrained thromboxane B(2) formation and Ca(2+) mobilization caused by AA, but failed to regulate 6-keto-prostaglandin F(1α) production and malonaldehyde content. Treatment of Br-NBP improved cAMP, cGMP levels and NO synthesis but prevented serotonin secretion and PF(4) release. Results suggested that Br-NBP inhibited rat platelet aggregation and that the anti-platelet activity was related to both arachidonic acid cascade and cGMP-NO signal path.
authors: Department of Clinical Pharmacology, School of Medicine, Zhengzhou University, Zhengzhou, Henan 450052, People's Republic of China.
authors: Feifei
authors: F
authors: Ma
authors: Yuan
authors: Y
authors: Gao
authors: Hailing
authors: H
authors: Qiao
authors: Xiangjie
authors: X
authors: Hu
authors: Junbiao
authors: J
authors: Chang
mesh_terms: D000818:Animals; D008297:Male; D010796:Phthalic Anhydrides / Q000494:pharmacology*; D010865:Pilot Projects; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010796:Phthalic Anhydrides; D010975:Platelet Aggregation Inhibitors; C026105:butylidenephthalide