title: Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis.
abstract: A thorough understanding of the events during mammalian spermatogenesis requires studying specific molecular signatures of individual testicular cell populations as well as their interaction in co-cultures. However, most purification techniques to isolate specific testicular cell populations are time-consuming, require large numbers of animals, and/or are only able to isolate a few cell types. Here we describe a cost-effective and timesaving approach that uses a single protocol to enrich multiple testicular cell populations (Sertoli, Leydig, and several spermatogenic cell populations) from as few as one mouse. Our protocol combines rigorous enzymatic digestion of seminiferous tubules with counter-current centrifugal elutriation, yielding specific testicular cell populations with >80%-95% purity.
authors: Greehey Children's Cancer Research Institute, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
authors: Yao-Fu
authors: YF
authors: Chang
authors: Jennifer S
authors: JS
authors: Lee-Chang
authors: Subbarayalu
authors: S
authors: Panneerdoss
authors: James A
authors: JA
authors: MacLean
authors: Manjeet K
authors: MK
authors: Rao
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000191:economics / Q000379:methods*; D007985:Leydig Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D012671:Seminiferous Tubules / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology*; D013093:Spermatogonia / Q000166:cytology*; D013737:Testis / Q000166:cytology*