title: Detection of extended-spectrum beta-lactamase-producing Escherichia coli isolates in faecal samples of Iberian lynx.
abstract: OBJECTIVE
  To characterize the diversity of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates recovered within the faecal microbiota of Iberian lynx. The identification of other associated resistance genes and the analysis of clonal relationship were also focused in this study.
  
  
  RESULTS
  From 2008 to 2010, 128 faecal samples of Iberian lynx (wild and captive animals) were collected. Eleven tested samples contained cefotaxime-resistant E. coli isolates (all belonging to captive animals) and 10 ESBL-producing isolates were showed. CTX-M-14 and SHV-12 ESBL-types were detected and seven different patterns were identified by pulsed-field gel electrophoresis analysis.
  
  
  CONCLUSIONS
  The occurrence of unrelated multiresistant E. coli in faecal flora of captive specimens of Iberian lynx, including the presence of ESBLs, resistant genes in integrons and virulence determinants was showed in this study.
  
  
  CONCLUSIONS
  The results obtained in this study highlight the environmental problem as future reintroductions of Iberian lynx could lead to a spread of resistant bacteria. Additionally, ESBL-producing bacteria can represent a health problem for this endangered species.
authors: Center of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
authors: A
authors: A
authors: Gonçalves
authors: G
authors: G
authors: Igrejas
authors: H
authors: H
authors: Radhouani
authors: V
authors: V
authors: Estepa
authors: E
authors: E
authors: Alcaide
authors: I
authors: I
authors: Zorrilla
authors: R
authors: R
authors: Serra
authors: C
authors: C
authors: Torres
authors: P
authors: P
authors: Poeta
mesh_terms: D000818:Animals; D002439:Cefotaxime; D003247:Conservation of Natural Resources; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D056727:Endangered Species; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D032023:Integrons; D046008:Lynx; D013030:Spain; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: C456080:beta-lactamase CTX-M-14; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases; D002439:Cefotaxime