title: Making reporter gene constructs to analyze cis-regulatory elements.
abstract: Cis-regulatory sequences control when, where, and how much genes are transcribed. A better understanding on these elements is a fundamental keystone to better understand development, cell differentiation, and morphogenesis. Several methods based on in silico analysis or ChIP-seq experiments have been developed to detect cis-acting sequences. Here, we describe a protocol to isolate such sequences from genomic DNA and to clone them into expression vectors for functional assays using the Gateway cloning technology.
authors: Centro Andaluz de Biología del Desarrollo (CABD), CSIC-Universidad Pablo de Olavide, Seville, Spain. jcribbes@upo.es
authors: José
authors: J
authors: Bessa
authors: José Luis
authors: JL
authors: Gómez-Skarmeta
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004742:Enhancer Elements, Genetic / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D016678:Genome / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D004247:DNA