title: Affinity-based chemoproteomics with small molecule-peptide conjugates.
abstract: In affinity-based chemoproteomics strategies, the direct immobilization of small bioactive probe molecules to a solid support may pose problems with respect to the preservation of the functional activity toward the target proteins. Typically, immobilized molecules on solid supports exhibit lower affinity for target proteins compared to the free parent molecule. This may lead to a failure to specifically capture the target proteins or to unacceptable losses during the washing steps. To circumvent these shortcomings, we have devised small molecule-peptide conjugates (SMPCs), which enable wide-ranging experimental strategies for the capturing of protein targets of small molecules from cells or tissues. With the possibilities of synthesizing peptides of tailored biochemical and biophysical properties, SMPCs enable the identification of protein targets of small molecules from cell-lysates and intact cells. Moreover, labeling of these conjugates with fluorophores can provide information on the cellular localization and distribution of the target.
authors: Shantani Proteome Analytics Pvt. Ltd., Pune, MH, India. csaxena@shantani.com
authors: Chaitanya
authors: C
authors: Saxena
mesh_terms: D015153:Blotting, Western; D002457:Cell Extracts; D002470:Cell Survival; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D015696:Gene Products, tat / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D008301:Maleimides / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D054852:Small Molecule Libraries / Q000378:metabolism*; D014966:Xanthenes / Q000737:chemistry / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D015696:Gene Products, tat; D007211:Indoles; D008301:Maleimides; D010455:Peptides; D054852:Small Molecule Libraries; C520544:Tat-octreotate-fluorescein 5'-carboxylic acid; D014966:Xanthenes; C088060:bisindolylmaleimide