title: 3,4-dihydroxyphenylalanine-assisted hydroxyapatite nanoparticle coating on polymer scaffolds for efficient osteoconduction.
abstract: For bone regeneration applications, scaffolds made from a composite of a biodegradable polymer and ceramic have advantages over scaffolds made from only one component (biodegradable polymer or ceramic alone). In this study, a simple and rapid method was developed to induce hydroxyapatite (HA) nanoparticle adsorption on polyglycolic acid (PGA) scaffold surfaces. PGA meshes were coated with HA nanoparticles by immersing the scaffolds in a buffer solution containing 3,4-dihydroxyphenylalanine (DOPA), a critical, functional element in mussel adhesive protein known to strongly bind to various materials. Substantial HA coating on PGA scaffolds was achieved within 24 hours of immersion, as determined according to selective staining of ceramic particles, scanning electron microscopy, X-ray photoelectron spectroscopy, and energy-dispersive spectroscopy. To evaluate the osteoconduction efficacy of the scaffolds in vivo, PGA scaffolds, DOPA-coated PGA scaffolds, PGA scaffolds immersed in HA solution, and HA- and DOPA-coated PGA (HA-DOPA-PGA) scaffolds were implanted in critical-sized defects in mouse skulls for 8 weeks. Micro-computed tomography and histological analyses showed that bone regeneration in vivo was far more extensive on HA-DOPA-PGA scaffolds than on the other scaffolds. DOPA offers an efficient and simple method of HA coating on polymer scaffolds. HA-polymer composite scaffolds fabricated using this method could be useful as bone graft.
authors: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors: Hee Seok
authors: HS
authors: Yang
authors: Jooyeon
authors: J
authors: Park
authors: Wan Geun
authors: WG
authors: La
authors: Hyeon-ki
authors: HK
authors: Jang
authors: Minhyung
authors: M
authors: Lee
authors: Byung-Soo
authors: BS
authors: Kim
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004295:Dihydroxyphenylalanine / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D020007:Implants, Experimental; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011100:Polyglycolic Acid / Q000494:pharmacology*; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D013052:Spectrometry, X-Ray Emission; D013718:Tensile Strength / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D020099:Coated Materials, Biocompatible; D011100:Polyglycolic Acid; D004295:Dihydroxyphenylalanine; D017886:Durapatite