title: [Variation of 4 kinds of compounds in Psoralea corylifolia processed by different methods].
abstract: OBJECITVE: To established an efficient method for simultaneous quantification of 7 compounds belonging to 4 chemical types in Psoralea corylifolia processed by different methods, and to elucidate variations of 4 kinds of compounds in different processed P. corylifolia.
  
  
  METHODS
  The chromatographic separation was performed on an ACUITY C18 column using acetonitrile and 0.1% formic acid aqueous solution in the gradient elution at 0.4 mL x min(-1). Detection wavelength was set at 246 nm. Column temperature was fixed at 50 degrees C.
  
  
  RESULTS
  The 4 kinds of compounds including psoralenoside, isopsoralenoside (benzofuran glycosides), psoralen, isopsoralen, psoralidin (coumarins), bavachin (flavonoids), and bakuchiol (meroterpenes) were separated in 25 min. The correlation coefficients of those compounds were over 0.9993 in the tested range. The intra- and inter-day precisions were below 1.5%. The average recoveries ranged from 99.2% to 106%.
  
  
  CONCLUSIONS
  This method is simple, rapid and accurate, which can be used for the determination of the 4 kinds of compounds in different processed P. corylifolia. In P. corylifolia processed by different methods, the contents of benzofuran glycosides, flavonoids, and meroterpenes in P. corylifolia processed by Leigong's method decrease obviously, while the contents of psoralen and isopsoralen increase significantly.
authors: Tianjin State Key Laboratory of Modern Chinese Medicine, Research Centre of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors: Xiao
authors: X
authors: Song
authors: Aidi
authors: A
authors: Qi
authors: Yuefei
authors: Y
authors: Wang
authors: Yakun
authors: Y
authors: Jing
authors: Xin
authors: X
authors: Chai
authors: Yanan
authors: Y
authors: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D010946:Plants, Medicinal / Q000737:chemistry*; D029907:Psoralea / Q000737:chemistry*; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical