title: [Polymorphism in coding region of pig PRDX6 gene and its genetic effects analysis].
abstract: PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Therefore, we used PRDX6 as an important candidate gene for meat quality according to its physiological and biochemical function. Partial coding sequence of porcine PRDX6 was isolated and two potenial SNPs, one at 417 bp (C/T) and the other at 423 bp (A/G), were found in the fourth exon by comparison of the obtained sequence from different pig breeds. In order to explore the relationship between PRDX6 polymorphism and meat quality, genetic variation and trait association of these two SNPs were separately performed in 6 purebred pig population and 247 F2 "Large White x Meishan" resource population by pyrosequencing. The results showed that allele C was predominant in western pig breeds, while allele T was predominant in Chinese indigenous breeds at 417 bp (C/T). This SNP was significantly associated with the intramuscular fat and water moisture (P < 0.05). The A/G mutation at 423 bp was significantly associated with drip water rate, water holding capacity, intramuscular fat, and water moisture (P < 0.05). Allele A was predominant in western pig breeds, while allele G was predominant in Chinese indigenous breeds. These two SNPs were likely to be important markers affecting meat quality traits (especially the muscle tenderness).
authors: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. liuyanghzau@163.com
authors: Yang
authors: Y
authors: Liu
authors: Wang-Jun
authors: WJ
authors: Wu
authors: Bo
authors: B
authors: Zuo
authors: Zhu-Qing
authors: ZQ
authors: Ren
authors: Yuan-Zhu
authors: YZ
authors: Xiong
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D005223:Fats / Q000032:analysis / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D008297:Male; D008460:Meat / Q000032:analysis; D008969:Molecular Sequence Data; D054465:Peroxiredoxin VI / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D013552:Swine / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D005223:Fats; D054465:Peroxiredoxin VI