title: Arabidopsis                      ACA7, encoding a putative auto-regulated Ca(2+)-ATPase, is required for normal pollen development.
abstract: Microgametogenesis is a complex process that involves numerous well-coordinated cell activities, ending with the production of pollen grains. Pollen development has been studied at the cytological level in Arabidopsis and other plant species, where its temporal time course has been defined. However, the molecular mechanism underlying this process is still unclear, since a relative small number of genes and/or processes have been identified as essential for pollen development. We have designed a methodology to select candidate genes for functional analysis, based on transcriptomic data obtained from different stages of pollen development. From our analyses, we selected At2g22950 as a candidate gene; this gene encodes a protein belonging to the auto-regulated Ca(2+)-ATPase family, ACA7. Microarray data indicate that ACA7 is expressed exclusively in developing pollen grains, with the highest level of mRNA at the time of the second pollen mitosis. Our RT-PCR experiments showed that ACA7 mRNA is detected exclusively in developing flowers. Confocal microscopy experiments showed a plasma membrane localization for the recombinant GFP:ACA7 protein. We identified two different insertional mutant lines, aca7-1 and aca7-2; plants from both mutant lines displayed a normal vegetative development but showed large amounts of dead pollen grains in mature flowers assayed by Alexander's staining. Histological analysis indicated that abnormalities are detected after the first pollen mitosis and we found a strong correlation between ACA7 mRNA accumulation and the severity of the phenotype. Our results indicate that ACA7 is a plasma membrane protein that has an important role during pollen development, possibly through regulation of Ca(2+) homeostasis.
authors: Laboratorio de Reproducción y Desarrollo de Plantas, Centro de Biotecnología Vegetal, Universidad Andres Bello, República 217, Santiago, Chile.
authors: Noel
authors: N
authors: Lucca
authors: Gabriel
authors: G
authors: León
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D035264:Flowers / Q000235:genetics / Q000254:growth & development; D005718:Gametogenesis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D008938:Mitosis; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011058:Pollen / Q000235:genetics / Q000254:growth & development*; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; C000589971:ACA7 protein, Arabidopsis; D000252:Calcium-Transporting ATPases