title: Diversity and killer activity of yeasts in Malaysian fermented food samples.
abstract: The biodiversity and the killer activity of yeasts isolated from various types of   fermented food in Malaysia were investigated in this study. Of 252 yeasts isolated from 48   fermented food samples in this study, 19 yeast species were identified based on sequence   analysis of the ITS1-5.8S-ITS2 partial fragments of the yeasts. A total of 29 (11.5%) of the   yeast isolates demonstrated killer activity to at least one Candida species tested in this   study; including 22 isolates of Trichosporon asahii, 4 isolates of Pichia anomala, and one   isolate each of Pichia norvegensis, Pichia fermentans and Issatchenkia orientalis,   respectively. The presence of killer yeasts reflects antagonism that occurs during microbial   interaction in the fermented food, whereby certain yeasts produce killer toxins and possibly   other toxic substances in competition for limited nutrients and space. The anti-Candida   activity demonstrated by killer yeasts in this study should be further explored for development   of alternative therapy against candidiasis.
authors: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 56000, Malaysia.
authors: S L
authors: SL
authors: Lim
authors: S T
authors: ST
authors: Tay
mesh_terms: D000898:Antibiosis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D020459:Genes, rRNA; D008296:Malaysia; D012331:RNA, Fungal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S