title: Expression of Toll-like receptors 2, 4 and 9 in patients with Guillain-Barré syndrome.
abstract: OBJECTIVE
  A myriad of transcription factors and inflammatory cytokines have been described to participate in the pathogenesis of Guillain-Barré syndrome (GBS). However, the innate immunity components--Toll-like receptors (TLRs)--have never been explored in this disease. We therefore investigated the expression of TLR2, 4 and 9 in the peripheral circulation of GBS patients as well as healthy controls.
  
  
  METHODS
  Twenty-one GBS patients and 21 healthy donors participated in this study. Peripheral blood mononuclear cells were used for mRNA and protein analysis of TLR-related molecules. Also, peripheral blood mononuclear cells from different subjects were incubated with different TLR agonists and the subsequent IFN-γ secretion was determined.
  
  
  RESULTS
  Expression of TLR2, 4 and 9 as well as their related signaling molecules were higher in GBS patients compared to healthy controls. Disability scores of GBS patients had a strong positive correlation with the high levels of expression of TLR2, 4 and 9.
  
  
  CONCLUSIONS
  The TLR signaling pathway may be involved in the pathogenesis of GBS.
authors: Department of Neurology, Xiangya Hospital, Central South University, Changsha, PR China.
authors: Yu-Zhong
authors: YZ
authors: Wang
authors: Qiu-Hua
authors: QH
authors: Liang
authors: Hhoonisha
authors: H
authors: Ramkalawan
authors: Yan-Li
authors: YL
authors: Wang
authors: Yan-Fang
authors: YF
authors: Yang
authors: Wen-Bin
authors: WB
authors: Zhou
authors: Fa-Fa
authors: FF
authors: Tian
authors: Jing
authors: J
authors: Li
authors: Huan
authors: H
authors: Yang
mesh_terms: D000328:Adult; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D020275:Guillain-Barre Syndrome / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013223:Statistics as Topic; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism*; D055815:Young Adult
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors