title: Screening and identification of a renal carcinoma specific peptide from a phage display peptide library.
abstract: BACKGROUND
  Specific peptide ligands to cell surface receptors have been extensively used in tumor research and clinical applications. Phage display technology is a powerful tool for the isolation of cell-specific peptide ligands. To screen and identify novel markers for renal cell carcinoma, we evaluated a peptide that had been identified by phage display technology.
  
  
  METHODS
  A renal carcinoma cell line A498 and a normal renal cell line HK-2 were used to carry out subtractive screening in vitro with a phage display peptide library. After three rounds of panning, there was an obvious enrichment for the phages specifically binding to the A498 cells, and the output/input ratio of phages increased about 100 fold. A group of peptides capable of binding specifically to the renal carcinoma cells were obtained, and the affinity of these peptides to the targeting cells and tissues was studied.
  
  
  RESULTS
  Through a cell-based ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, the Phage ZT-2 and synthetic peptide ZT-2 were shown to specifically bind to the tumor cell surfaces of A498 and incision specimens, but not to normal renal tissue samples.
  
  
  CONCLUSIONS
  A peptide ZT-2, which binds specifically to the renal carcinoma cell line A498 was selected from phage display peptide libraries. Therefore, it provides a potential tool for early diagnosis of renal carcinoma or targeted drug delivery in chemotherapy.
authors: Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, Guangdong, PR China. txabs9988@163.com
authors: Xiangan
authors: X
authors: Tu
authors: Jintao
authors: J
authors: Zhuang
authors: Wenwei
authors: W
authors: Wang
authors: Liang
authors: L
authors: Zhao
authors: Liangyun
authors: L
authors: Zhao
authors: Jiquan
authors: J
authors: Zhao
authors: Chunhua
authors: C
authors: Deng
authors: Shaopeng
authors: S
authors: Qiu
authors: Yuanyuan
authors: Y
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D010455:Peptides; C570845:peptide ZT-2