title: Differentiation between fresh beef and thawed frozen beef.
abstract: In order to prevent meat retailers offering thawed, imported frozen beef as fresh domestic beef, the method of Gottesmann and Hamm for differentiating between fresh and frozen/thawed meat is recommended. The principle of this method is based on the fact that freezing and thawing of meat results in a release of the enzyme β-hydroxyacyl CoA-dehydrogenase (HADH) from the mitochondrion into the sarcoplasm; an elevated HADH activity in the muscle press-juice indicates freezing and thawing of tissue. The HADH colour test of Gottesmann and Hamm was modified by replacing the electron-transmitter meldolablue by resazurin which results in a much higher colour stability after reaction with fresh meat extracts.
authors: Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.
authors: M T
authors: MT
authors: Chen
authors: W D
authors: WD
authors: Yang
authors: S L
authors: SL
authors: Guo