title: The solution structure of the S4-S5 linker of the hERG potassium channel.
abstract: The human ether-à-go-go related gene (hERG) encodes a protein that forms a voltage-gated potassium channel and plays an important role in the heart by controlling the rapid delayed rectifier K(+) current (I(Kr)). The S4-S5 linker was shown to be important for the gating of the hERG channel. Nuclear magnetic resonance study showed that a peptide derived from the S4-S5 linker had no well-ordered structure in aqueous solution and adopted a 3(10) -helix (E544-Y545-G546) structure in detergent micelles. The existence of an amphipathic helix was confirmed, which may be important for interaction with cell membrane. Close contact between side chains of residues R541 and E544 was observed, which may be important for its regulation of channel gating.
authors: Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), Singapore 138669, Singapore.
authors: Shovanlal
authors: S
authors: Gayen
authors: Qingxin
authors: Q
authors: Li
authors: CongBao
authors: C
authors: Kang
mesh_terms: D020816:Amino Acid Motifs; D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008958:Models, Molecular; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013499:Surface Properties; D015534:Trans-Activators / Q000737:chemistry*; D000071230:Transcriptional Regulator ERG
chemical_list: C072312:ERG protein, human; D008823:Micelles; D010446:Peptide Fragments; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG