title: Single nuclei Raman spectroscopy for drug evaluation.
abstract: Detection of cellular changes at single-cell level has a great potential for biomedical and biopharmaceutical applications. Raman spectroscopy is an important tool for single-cell molecular imaging analysis. Raman spectroscopy can provide time-resolved information of the selected biomolecular distributions inside a single cell without the need of chemical labeling. In this study, we monitored the cellular responses to antineoplastic drug at a single cell basis with Raman spectroscopy. We demonstrated that single nuclei Raman spectroscopy has the ability to detect and identify nuclear changes related to cytotoxicity at lower concentrations and in shorter time span than conventional cell based assays. Thus, this strategy of using Raman spectroscopy of single, isolated nuclei may be very valuable for rapid and sensitive detection of cellular changes in response to chemotherapeutic agents.
authors: Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors: Hsin-Hung
authors: HH
authors: Lin
authors: Yen-Chang
authors: YC
authors: Li
authors: Chih-Hao
authors: CH
authors: Chang
authors: Chun
authors: C
authors: Liu
authors: Alice L
authors: AL
authors: Yu
authors: Chung-Hsuan
authors: CH
authors: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents