title: [Molecular mechanism of acupoint catgut-embedding for experimental colitis in rats].
abstract: OBJECTIVE
  To explore the effect and its mechanism of acupoint catgut-embedding for experimental colitis in rats.
  
  
  METHODS
  Eighteen SD rats were randomly divided into a normal control (NC) group, a model (MO) group and a acupoint catgut-embedding (AC) group, 6 rats in each group. Animals in MO group and AC group were treated by trinitro-benzene-sulfonic acid (TNBS) to establish model with colitis. No other treatment was given to the rats in MO group, but acupoint catgut-embedding was implanted at "Shangjuxu" (ST 37), "Tianshu" (ST 25) and "Dachangshu" (BL 25) in the rats in AC group. The symptoms of diarrhea and bloody stool, and histopathology changes in colon were observed 15 days after the treatment. Expression of IL-17, beta2AR, NFkappaBp65 were observed by immunohistochemistry. Expressions of NF-kappaBp65 and beta2AR in splenic lymphocyte were detected by the Western blot method.
  
  
  RESULTS
  Diarrhea and mucus bloody purulent stool were soon controlled, and colon mucosa injures were obviously improved in AC group. The NF-kappaBp65 value in splenic lymphocytes of 249.70 +/- 13.66 in MO) group was higher than 86.22 +/- 8.09 in NC group (P < 0.01), and 219.02 +/- 7.42 in AC group was less than that in MO group (P < 0.01). The expression of beta2AR in splenic lymphocytes of 594.97 +/- 173.22 in MO group was less than 957.45 +/- 171.56 in NC group (P < 0.01), and 1335.93 +/- 244.34 in AC group was higher than that in MO group (P < 0.01). The expression of IL-17 in colon mucosa in MO group was increased, while the expression of IL-17 in colon mucosa in AC group was decreased.
  
  
  CONCLUSIONS
  Acupoint catgut-embedding at Shangjuxu (ST 37), Tianshu (ST 25) and Dachangshu (BL 25) has obviously effect in treating experimental colitis and the mechanism may be related to regulate the expression of IL-17, beta2AR and NF-kappaBp65.
authors: Department of Integrated Chinese and Western Medicine, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors: Xia-Yi
authors: XY
authors: Zhang
authors: Lin
authors: L
authors: Shen
authors: Heng
authors: H
authors: Fan
authors: Yi
authors: Y
authors: Liao
authors: Li
authors: L
authors: Liang
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D002398:Catgut; D003092:Colitis / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D020381:Interleukin-17; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D016328:NF-kappa B; D011944:Receptors, Androgen