title: In silico feedback for in vivo regulation of a gene expression circuit.
abstract: We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.
authors: Department of Electrical Engineering, ETH Zurich, Automatic Control Laboratory, Zurich, Switzerland.
authors: Andreas
authors: A
authors: Milias-Argeitis
authors: Sean
authors: S
authors: Summers
authors: Jacob
authors: J
authors: Stewart-Ornstein
authors: Ignacio
authors: I
authors: Zuleta
authors: David
authors: D
authors: Pincus
authors: Hana
authors: H
authors: El-Samad
authors: Mustafa
authors: M
authors: Khammash
authors: John
authors: J
authors: Lygeros
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D005246:Feedback; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics*; D008957:Models, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D049490:Systems Biology