title: [Quantitative determination of peimisin and total alkaloids in Fritillaria taipaiensis of different growing stage].
abstract: OBJECTIVE
  To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage.
  
  
  METHODS
  High performance liquid chromatography coupled with evaporative light scattering detector was employed, and peimisine was separated and quantified upon an Agilent Extend C18 (250 mm x 4.0 mm, 5 microm), using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL min(-1); UV spectrophotometry was used for the determination of total alkaloids.
  
  
  RESULTS
  During from two to three years period, the content of peimisine in F. taipaiensis increased with growing stage, from four to six years, it decreased with growing stage; From two to four years, the content of total alkaloids in F. taipaiensis increased with growing stage, from five to six years, it decreased with growing stage.
  
  
  CONCLUSIONS
  It is suitable to harvest F. taipaienssi of four years growing stage.
authors: Mianyang Normal University, Mianyang 621000, China. why0929@126.com
authors: Huai-yu
authors: HY
authors: Wang
authors: Peng
authors: P
authors: Ma
authors: Rui
authors: R
authors: Peng
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis; D031410:Fritillaria / Q000737:chemistry* / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012621:Seasons; D013997:Time Factors
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C052510:peimisine