title: Analysis of transcriptional factors and regulation networks in laryngeal squamous cell carcinoma patients with lymph node metastasis.
abstract: The present study was to identify and quantitate differentially expressed proteins in laryngeal squamous cell carcinoma (LSCC) tissues with or without lymph node metastasis and to explore transcriptional factors and regulation networks associated with the process. Tissue specimens were taken from 20 patients with LSCC, including 10 cases of LSCC without metastasis LSCC (N0) and 10 cases of LSCC with metastasis LSCC (Nx). Among the 643 unique proteins identified by using iTRAQ labeling and quantitative proteomic technology, 389 proteins showed an abundance change in LSCC (Nx) as compared to LSCC (N0). Cytoskeleton remodeling, cell adhesion, and immune response activation were found to be the main processes in LSCC metastasis. The construction of transcription regulation networks identified key transcription regulators for lymph node metastasis of LSCC, including Sp1, c-myc, and p53, which may affect LSCC metastasis through the epithelial-mesenchymal transition. Furthermore, our results suggest that ubiquitination may be a critical factor in the networks. The present study provides insights into transcriptional factors and regulation networks involved in LSCC metastasis, which may lead to new strategies for treatment of LSCC metastasis.
authors: Department of Otolaryngology-Head Neck Surgery, Xiangya Hospital, Central South University , 87 Xiangya Road, Changsha, Hunan, PR China 410008.
authors: Geng-Ming
authors: GM
authors: Cai
authors: Dong-Hai
authors: DH
authors: Huang
authors: Yao-Zhang
authors: YZ
authors: Dai
authors: Yong
authors: Y
authors: Liu
authors: Lei-Ming
authors: LM
authors: Pi
authors: Hao-Lei
authors: HL
authors: Tan
authors: Li-Jun
authors: LJ
authors: Liu
authors: Yun-Xia
authors: YX
authors: Lv
authors: Chang-Yun
authors: CY
authors: Yu
authors: Ping-Qing
authors: PQ
authors: Tan
authors: Yong-Quan
authors: YQ
authors: Tian
authors: Georgia Z
authors: GZ
authors: Chen
authors: Xin
authors: X
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060066:Protein Interaction Maps; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D014157:Transcription Factors / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D016329:Sp1 Transcription Factor; C495901:TP53 protein, human; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53