title: Hydrogels containing rutin intended for cutaneous administration: efficacy in wound healing in rats.
abstract: OBJECTIVE
  Development of a hydrogel containing rutin at 0.025% (w/w) and evaluation of its in vivo efficacy in cutaneous wound healing in rats.
  
  
  METHODS
  Hydrogels were prepared using Carbopol Ultrez® 10 NF and an aqueous dispersion of rutin in polysorbate 80. Hydrogels were characterized by means of pH measurement, rheological and spreadability analysis and rutin content determination by liquid chromatography. The in vivo healing effect was evaluated through the regression of skin lesions in rats and by analysis of oxidative stress.
  
  
  CONCLUSIONS
  Hydrogels showed adequate pH values (5.50-6.50) and pseudoplastic non-Newtonian behavior. After 5 days of treatment of wounds, hydrogels containing rutin presented a higher decrease in the wound area compared to the control hydrogels. Analysis of the oxidative stress showed a decrease in lipid peroxidation and protein carbonyl content as well as an increase in catalase activity after the treatment with the hydrogel containing rutin. Furthermore, this treatment increased total protein levels.
  
  
  CONCLUSIONS
  This study shows for the first time the feasibility of using dermatological formulations containing rutin to improve skin wound healing.
authors: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, Brazil.
authors: J S
authors: JS
authors: Almeida
authors: D M
authors: DM
authors: Benvegnú
authors: N
authors: N
authors: Boufleur
authors: P
authors: P
authors: Reckziegel
authors: R C S
authors: RC
authors: Barcelos
authors: K
authors: K
authors: Coradini
authors: L M
authors: LM
authors: de Carvalho
authors: M E
authors: ME
authors: Bürger
authors: R C R
authors: RC
authors: Beck
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000279:Administration, Cutaneous; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D004195:Disease Models, Animal; D005240:Feasibility Studies; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011136:Polysorbates / Q000737:chemistry*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D012431:Rutin / Q000008:administration & dosage* / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; D011136:Polysorbates; C578916:Ultrez 10; D012431:Rutin