title: Cloning and expression analysis of piRNA-like RNAs: adult testis-specific small RNAs in chicken.
abstract: Many small RNAs have been cloned from animal gonads, for example, endogenous small interfering RNAs (endo-siRNAs) were found in oocytes and piwi-interacting RNAs (piRNAs) were found in testis. Gallus gallus (chicken) is an important model organism, but few small RNAs have been identified from its gonads. In this study, we isolated and cloned 156 small RNAs from adult chicken testes. Since there is a reasonably even distribution from 22 to 33 nt, these small RNAs are slightly longer than miRNAs and endo-siRNAs. Genome mapping indicated that these small RNAs were derived from intergenic regions, exons, introns, and repetitive elements including chicken repeat 1, long terminal repeats, and simple repeats. Since they are similar with piRNAs, we named them piRNA-like RNAs (pil-RNAs). Northern blotting of 16 selected sequences showed that nine are specifically expressed in the adult testis. The vast majority of these pil-RNAs are poorly conserved between species, suggesting that they are unique to the adult chicken testis. Further analysis of the cloned pil-RNAs will improve our understanding of the function of small RNAs in animal gonad development.
authors: State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing, People's Republic of China.
authors: Hui
authors: H
authors: Yang
authors: Xiaobo
authors: X
authors: Wang
authors: Xiaojuan
authors: X
authors: Liu
authors: Xiaofang
authors: X
authors: Liu
authors: Lingxiao
authors: L
authors: Li
authors: Xiaoxiang
authors: X
authors: Hu
authors: Ning
authors: N
authors: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering