title: Vitamin E reduces hepatic fibrosis in mice with Schistosoma japonicum             infection.
abstract: To investigate whether vitamin E protects against hepatic fibrosis in mice with Schistosoma japonicum infection, 24 pathogen-free Kunming mice were selected and randomly divided into four groups: control (uninfected, untreated), model (infected, untreated), low-dose intervention (infected, vitamin E-treated, 30 mg/g bodyweight/day) and high-dose intervention (infected, vitamin E-treated, 60 mg/g bodyweight/day). Mice were infected with Schistosoma japonicum by inoculating abdominal skin with snail hosts. The activities of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were detected in hepatic tissue by colorimetry. The expression levels of laminin (LN), hyaluronic acid (HA), procollagen type Ⅲ (PC-III) and type Ⅳ collagen (IV-C) were detected in the serum by radioimmunoassay. Finally, areas and numbers of granulomas were assessed through histopathology 42 days following treatment. The results revealed that mean areas of granulomas were smaller in the low- and high-dose intervention groups compared to those in the model group. Furthermore, the higher dose of vitamin E resulted in smaller granulomas than the low dose. The levels of LN, HA, PC-III and IV-C in the serum were lower following vitamin E treatment than in the model group. By contrast, activity of SOD, GPx and CAT in hepatic tissue was higher following vitamin E treatment compared to the model group. The activity of MDA was lower in hepatic tissue following vitamin E treatment compared to the model group, but was higher compared to controls. In general, the higher dose of vitamin E affected measurements to a greater extent than the lower dose. In conclusion, vitamin E treatment may reduce the growth of granulomas, slowing the process of hepatic fibrosis, and this effect may be the result of the altered activity of the oxidation-reduction enzyme system.
authors: Department of Pathogen Biology and Immunology, School of Medicine, Anhui University of Science and Technology, Anhui 232001, PR China. wangxxff1023@126.com
authors: Xuefeng
authors: X
authors: Wang
authors: Rongbo
authors: R
authors: Zhang
authors: Jiuwei
authors: J
authors: Du
authors: Youying
authors: Y
authors: Hu
authors: Lifa
authors: L
authors: Xu
authors: Jun
authors: J
authors: Lu
authors: Song
authors: S
authors: Ye
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D024061:Collagen Type III / Q000097:blood; D024141:Collagen Type IV / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006820:Hyaluronic Acid / Q000097:blood; D007797:Laminin / Q000097:blood; D008099:Liver / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000150:complications* / Q000378:metabolism / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D012549:Schistosoma japonicum / Q000502:physiology; D012554:Schistosomiasis japonica / Q000150:complications* / Q000201:enzymology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D024061:Collagen Type III; D024141:Collagen Type IV; D007797:Laminin; D014815:Vitamins; D014810:Vitamin E; D008315:Malondialdehyde; D006820:Hyaluronic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase