title: The similarities between the activities of the calcium activated sarcoplasmic factor and the kinase activating factor from rabbit skeletal muscle.
abstract: The similarity, that has been suggested by other investigators, between the kinase activating factor (KAF) and the calcium activated sarcoplasmic factor (CASF) of rabbit skeletal muscle is extended through a comparison of enzyme activities. Proteolytic activity on casein, the ability to activate skeletal muscle glycogen phosphorylase b kinase, inhibition by bovine heart KAF inhibitor and effects on myofibrillar integrity were examined using these two skeletal muscle proteases and a typical bacterial protease isolated from Pseudomonas perolens (ATCC 10757). The study demonstrated that the skeletal muscle proteases have more in common with each other than either protease has with the bacterial protease. The ability to influence the integrity of the myofibril was demonstrated by phase-contrast microscopy and SDS polyacrylamide gel electrophoresis. The KAF and CASF demonstrated similar effects that again differed from the bacterial protease effects.
authors: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA.
authors: G J
authors: GJ
authors: Seperich
authors: J F
authors: JF
authors: Price