title: Self-assembly of renal cells into engineered renal tissues in collagen/Matrigel scaffold in vitro.
abstract: To tissue engineer a kidney is a formidable task because of the complex cell composition and structures in the kidney. This study reconstructed renal tissues using mixed renal cells in collagen/Matrigel® scaffolds in vitro. Neonatal rat renal cells were seeded in collagen I supplemented with Matrigel in a casting mold that could exert static stretch when the renal constructs contracted. During in vitro culture, the renal constructs were observed under microscope and analyzed by histological and immunofluorescent examinations. Results showed that the mixed renal cells reconstituted renal tubular and glomeruli-like structures with different appearances at varying developmental stages. Tubular structures were formed by CK18-positive cells with similar appearances lining the surrounding hollow centres. The glomeruli-like structures were tufts of cell aggregates containing Flk-1-positive cells. These results show that neonatal rat renal cells self-assembled into engineered renal tissues containing both tubules and glomeruli-like structures when cultured in 3D collagen/Matrigel scaffold in vitro.
authors: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, China.
authors: Shuang Hong
authors: SH
authors: Lü
authors: Qiuxia
authors: Q
authors: Lin
authors: Yu Na
authors: YN
authors: Liu
authors: Qun
authors: Q
authors: Gao
authors: Tong
authors: T
authors: Hao
authors: Yan
authors: Y
authors: Wang
authors: Jin
authors: J
authors: Zhou
authors: Haibin
authors: H
authors: Wang
authors: Zhiyan
authors: Z
authors: Du
authors: Jie
authors: J
authors: Wu
authors: Chang Yong
authors: CY
authors: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D004338:Drug Combinations; D007678:Kidney Glomerulus / Q000166:cytology* / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology*; D011509:Proteoglycans / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen