title: Temporal investigation of matrix metalloproteinases and their inhibitors in human cervicovaginal fluid in late pregnancy and labor.
abstract: Temporal expression of matrix metalloproteinase (MMP)-1, -2, -3, -7, -8, -9, -12, and -13, and tissue inhibitors of metalloproteinases (TIMPs)-1 and -2 in human cervicovaginal fluid (CVF) in term pregnancy and labor was investigated. Term parous women provided CVF samples that were grouped into labor, 1 to 3, 6 to 8, and 12 to 16 days before labor onset. Both MMPs and TIMPs (n = 60) were quantified using multiplex solution array and enzyme-linked immunosorbent assays, respectively. Further analysis of TIMP-1 (n = 180) was undertaken. All MMPs and TIMPs except MMP-12 and -13 were detected in the CVF. Matrix metalloproteinase 7, TIMP-1, and TIMP-2 were significantly increased in labor. Tissue inhibitors of metalloproteinase 1 was significantly increased up to 7 days before spontaneous labor onset. The data suggest a role of MMP-7 in the remodeling and rupture of fetal membranes and may reflect the homeostatic regulation of extracellular matrix remodeling of MMP-7 by TIMP-1 and TIMP-2.
authors: Department of Obstetrics and Gynaecology, The University of Melbourne, Parkville, Victoria, Australia.
authors: Yujing J
authors: YJ
authors: Heng
authors: Megan K W
authors: MK
authors: Di Quinzio
authors: Stella
authors: S
authors: Liong
authors: Michael
authors: M
authors: Permezel
authors: Gregory E
authors: GE
authors: Rice
authors: Harry M
authors: HM
authors: Georgiou
mesh_terms: D000653:Amniotic Fluid / Q000201:enzymology / Q000378:metabolism; D002584:Cervix Uteri / Q000201:enzymology / Q000378:metabolism; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007743:Labor, Obstetric / Q000378:metabolism*; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D012189:Retrospective Studies; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*; D014621:Vagina / Q000201:enzymology / Q000378:metabolism
chemical_list: C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7