title: Promicromonospora thailandica sp. nov., isolated from marine sediment.
abstract: A novel actinomycete, strain S7F-02(T), which produced primary branched hyphae and fragmented into V- and Y-shaped bacillary cells, was isolated from marine sediment collected in the Andaman Sea, Trang Province, Thailand. Lysine was found to be the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars of strain S7F-02(T) were ribose, arabinose and glucose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H(4)). The predominant cellular fatty acids were anteiso-C(15 : 0) and iso-C(15 : 0). The G+C content of the genomic DNA of strain S7F-02(T) was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7F-02(T) should be classified in the genus Promicromonospora. This strain formed a close association with Promicromonospora citrea DSM 43110(T), with which it shared 99.2 % 16S rRNA gene sequence similarity. DNA-DNA hybridization data together with physiological and biochemical properties showed that strain S7F-02(T) could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain S7F-02(T) is considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora thailandica sp. nov. is proposed. The type strain is S7F-02(T) (= BCC 41922(T) = JCM 17130(T)).
authors: Department of Biology and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
authors: Chitti
authors: C
authors: Thawai
authors: Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors: Takuji
authors: T
authors: Kudo
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013785:Thailand; D014871:Water Microbiology
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S