title: [Expression and significance of LMP2 and PPM1A in gestational trophoblastic disease].
abstract: OBJECTIVE
  To investigate the expression of low molecular mass polypeptide-2 (LMP2) and protein phosphatase 1A (PPM1A) in gestational trophoblastic disease and elucidate their predictive value in malignant transformation of hydatidiform mole.
  
  
  METHODS
  The expressions of LMP2 and PPM1A protein in 196 complete hydatidiform moles (in which 28 cases with malignant transformation), 7 invasive moles, 5 choriocarcinomas and 20 normal chorionic villus were detected with the method of EnVision immunohistochemistry. Their clinicopathologic data were retrospectively analyzed.
  
  
  RESULTS
  LMP2 and PPM1A protein expressed in cytotrophocytes, syncytiotrophoblast and extravillous trophoblast. The level of LMP2 expression in deteriorative hydatidiform mole was significantly higher than that in non-deteriorative hydatidiform mole or normal chorionic villus (6.79±2.38, 5.26±2.63 and 3.10±1.65, all P<0.01), while there were no difference compared with gestational trophoblastic neoplasms (6.42±2.68, P=0.113). The level of PPM1A expression was highest in normal chorionic villus, and decreased gradually in hydatidiform mole (non-deteriorative and deteriorative) and gestational trophoblastic neoplasms (6.30±2.98, 4.93±2.50, 4.43±2.04 and 3.33±2.06, all P<0.01); the level of PPM1A expression in deteriorative hydatidiform mole was significantly lower than that in non-deteriorative hydatidiform mole (P=0.001). The expression of LMP2 protein was correlated to theca lutein ovarian cyst, the expression of PPM1A protein was related with uterine size (P<0.05). While, there was no correlation between the expressions of the two proteins (P>0.05).
  
  
  CONCLUSIONS
  High expression of LMP2 and low expression of PPM1A might play an important role in the motility and invasiveness of trophoblast cells and malignant transformation of hydatidiform mole. Testing the expression of LMP2 and PPM1A in hydatidiform mole tissues of initial uterine evacuation might be have some reference significance in judging outcomes of hydatidiform mole.
authors: Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors: Sha-sha
authors: SS
authors: Zhang
authors: Xiao-yun
authors: XY
authors: Wan
authors: Cai-yun
authors: CY
authors: Zhou
authors: Wei-guo
authors: WG
authors: Lü
authors: Xing
authors: X
authors: Xie
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D005260:Female; D031901:Gestational Trophoblastic Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008875:Middle Aged; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011237:Predictive Value of Tests; D011247:Pregnancy; D000071636:Protein Phosphatase 2C; D012189:Retrospective Studies; D014327:Trophoblasts / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: C071313:LMP-2 protein; C000607456:PPM1A protein, human; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; D003546:Cysteine Endopeptidases