title: Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides.
abstract: The mouse otocyst, an anlage of the inner ear, is an attractive experimental target for developing treatment modalities for congenital inner ear diseases and for studying inner ear development. Poly-arginine (6-12 residues) is a cell-penetrating peptide and can be used to deliver cargo into cells. Here, we achieved transutero delivery of enhanced green fluorescent protein (EGFP) fused to a nine-arginine peptide into mouse embryonic otocysts. The EGFP signal was detected both in the lining cells of the otocysts and in their vicinity at 18 h post injection. Mice injected with EGFP fused to a nine-arginine peptide had normal auditory and vestibular functions. These data suggest that protein transduction using poly-arginine may be a useful alternative strategy to commonly used gene delivery methods for delivering therapeutically relevant molecules to the developing inner ear.
authors: Department of Otolaryngology-Head and Neck Surgery, Kumamoto University, Graduate School of Medicine, Kumamoto University, Kumamoto, Japan.
authors: Toru
authors: T
authors: Miwa
authors: Ryosei
authors: R
authors: Minoda
authors: Taku
authors: T
authors: Kaitsuka
authors: Momoko
authors: M
authors: Ise
authors: Kazuhito
authors: K
authors: Tomizawa
authors: Eiji
authors: E
authors: Yumoto
mesh_terms: D000818:Animals; D057846:Cell-Penetrating Peptides / Q000494:pharmacology*; D007758:Ear, Inner / Q000166:cytology* / Q000196:embryology; D004622:Embryo, Mammalian; D018913:Event-Related Potentials, P300 / Q000502:physiology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D021381:Protein Transport / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D057846:Cell-Penetrating Peptides; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins