title: Quercetin inhibits IL-1β-induced inflammation, hyaluronan production and adipogenesis in orbital fibroblasts from Graves' orbitopathy.
abstract: Management of Graves' orbitopathy (GO) is challenging, as no reliable, specific, and safe medical therapeutic agents have yet been developed. We investigated the effect of quercetin in primary cultured orbital fibroblasts from GO, targeting pathways of inflammation, aberrant accumulation of extracellular matrix macromolecules, and adipose tissue expansion. Quercetin significantly attenuated intercellular adhesion molecule-1 (ICAM-1), interleukin (IL) -6, IL-8, and cyclooxygenase (COX) -2 mRNA expression, and inhibited IL-1β-induced increases in ICAM-1, IL-6, and IL-8 mRNA. Increased hyaluronan production induced by IL-1β or tumor necrosis factor-α was suppressed by quercetin in a dose- and time-dependent manner. Treatment with noncytotoxic doses of quercetin inhibited accumulation of intracytoplasmic lipid droplets and resulted in a dose-dependent decrease in expression of peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein (C/EBP) α, and C/EBPβ proteins. In conclusion, inhibition of inflammation, hyaluronan production, and adipogenesis by the natural plant product quercetin in vitro provides the basis for further study of its potential use in the treatment of GO.
authors: Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
authors: Jin Sook
authors: JS
authors: Yoon
authors: Hyun Jung
authors: HJ
authors: Lee
authors: Soo Hyun
authors: SH
authors: Choi
authors: Eun-Ju
authors: EJ
authors: Chang
authors: Sang Yeul
authors: SY
authors: Lee
authors: Eun Jig
authors: EJ
authors: Lee
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D006111:Graves Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007249:Inflammation / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D009915:Orbit / Q000166:cytology / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; D014157:Transcription Factors; D006820:Hyaluronic Acid; D011794:Quercetin