title: Genotyping with Sequenom.
abstract: Often in evolutionary genetics research, one needs to analyze polymorphisms in populations for which cost-efficient high-throughput arrays are nonexistent, either because the species is not a model organism or because the populations have been subjected to such specific conditions that their base variation is almost unique. In this situation, custom-made genotyping assays are required. Sequenom's MassARRAY(®) genotyping platform is a powerful and flexible method for assaying up to a few thousand markers and up to thousands of individuals. It is based on distinguishing allele-specific primer extension products by mass spectrometry (MALDI-TOF). Most stages of the experimental protocol reflect adaptations of established PCR protocols to multiplexing, which allows the simultaneous amplification and detection of multiple markers per reaction.
authors: Department of Biology, New York University, New York, NY, USA. mb3188@nyu.edu
authors: Martina
authors: M
authors: Bradić
authors: João
authors: J
authors: Costa
authors: Ivo M
authors: IM
authors: Chelo
mesh_terms: D019295:Computational Biology / Q000379:methods*; D017931:DNA Primers / Q000378:metabolism; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers