title: Quantitative proteomics reveals cellular targets of celastrol.
abstract: Celastrol, a natural substance isolated from plant extracts used in traditional Chinese medicine, has been extensively investigated as a possible drug for treatment of cancer, autoimmune diseases, and protein misfolding disorders. Although studies focusing on celastrol's effects in specific cellular pathways have revealed a considerable number of targets in a diverse array of in vitro models there is an essential need for investigations that can provide a global view of its effects. To assess cellular effects of celastrol and to identify target proteins as biomarkers for monitoring treatment regimes, we performed large-scale quantitative proteomics in cultured human lymphoblastoid cells, a cell type that can be readily prepared from human blood samples. Celastrol substantially modified the proteome composition and 158 of the close to 1800 proteins with robust quantitation showed at least a 1.5 fold change in protein levels. Up-regulated proteins play key roles in cytoprotection with a prominent group involved in quality control and processing of proteins traversing the endoplasmic reticulum. Increased levels of proteins essential for the cellular protection against oxidative stress including heme oxygenase 1, several peroxiredoxins and thioredoxins as well as proteins involved in the control of iron homeostasis were also observed. Specific analysis of the mitochondrial proteome strongly indicated that the mitochondrial association of certain antioxidant defense and apoptosis-regulating proteins increased in cells exposed to celastrol. Analysis of selected mRNA transcripts showed that celastrol activated several different stress response pathways and dose response studies furthermore showed that continuous exposure to sub-micromolar concentrations of celastrol is associated with reduced cellular viability and proliferation. The extensive catalog of regulated proteins presented here identifies numerous cellular effects of celastrol and constitutes a valuable biomarker tool for the development and monitoration of disease treatment strategies.
authors: Research Unit for Molecular Medicine, Aarhus University Hospital, Skejby, Aarhus, Denmark. Jakob.Hansen@ki.au.dk
authors: Jakob
authors: J
authors: Hansen
authors: Johan
authors: J
authors: Palmfeldt
authors: Søren
authors: S
authors: Vang
authors: Thomas J
authors: TJ
authors: Corydon
authors: Niels
authors: N
authors: Gregersen
authors: Peter
authors: P
authors: Bross
mesh_terms: D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000502:physiology; D053978:Pentacyclic Triterpenes; D011506:Proteins / Q000032:analysis* / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; D024101:Mitochondrial Proteins; D053978:Pentacyclic Triterpenes; D011506:Proteins; D012333:RNA, Messenger; D014315:Triterpenes; C050414:celastrol