title: [Effects of remifentanil combined with naloxone on human sperm motility].
abstract: OBJECTIVE
  To observe the effects of remifentanil combined with naloxone on human sperm motility in vitro and to investigate its possible mechanism.
  
  
  METHODS
  Twenty normal semen samples were collected, each divided into 13 aliquots, one as the control and the others treated in vitro with different concentrations of remifentanil or remifentanil + naloxone for 35 min. The percentage of progressive mobile sperm was assessed by computer-assisted sperm analysis at 5, 10, 15, 20 and 35 min.
  
  
  RESULTS
  Compared with the control group, remifentanil at 0.1, 1, 10 and 100 microg/L significantly decreased sperm motility at 5 and 10 min in a dose-dependent manner, with no significant difference at 15 and 30 min; sperm motility showed no significant difference on 5 -35 min exposure to naloxone at 0.004 -0.04 mg/L, nor on 5, 10, 15 and 20 min exposure at 0.4 -4 mg/L, but was significantly increased at 35 min. Compared with the corresponding concentrations of remifentanil alone, remifentanil + naloxone remarkably increased sperm motility at 0.1 + 0.004, 1 + 0.04, 10 + 0.4, and 100 + 4 mg/L at 5 and 10 min, with no obvious difference at 15 and 30 min.
  
  
  CONCLUSIONS
  The onset and offset of the effect of remifentanil on sperm motility are rapid and its inhibitory effect can be antagonized by naloxone, which may be related with the micro-opioid receptor.
authors: Department of Anesthesiology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China. xubo1028@sina.com
authors: Bo
authors: B
authors: Xu
authors: Yan-juan
authors: YJ
authors: Wang
authors: Zhi-ping
authors: ZP
authors: Wang
authors: Yi-ping
authors: YP
authors: Hu
authors: Xiao-hai
authors: XH
authors: Wang
authors: Qin
authors: Q
authors: Li
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D009270:Naloxone / Q000008:administration & dosage / Q000494:pharmacology*; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology*; D000077208:Remifentanil; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D055815:Young Adult
chemical_list: D010880:Piperidines; D009270:Naloxone; D000077208:Remifentanil