title: The transcriptional regulators Id2 and Id3 control the formation of distinct memory CD8+ T cell subsets.
abstract: During infection, naive CD8(+) T cells differentiate into effector cells, which are armed to eliminate pathogens, and memory cells, which are poised to protect against reinfection. The transcriptional program that regulates terminal differentiation into short-lived effector-memory versus long-lived memory cells is not clearly defined. Through the use of mice expressing reporters for the DNA-binding inhibitors Id2 and Id3, we identified Id3(hi) precursors of long-lived memory cells before the peak of T cell population expansion or upregulation of cell-surface receptors that indicate memory potential. Deficiency in Id2 or Id3 resulted in loss of distinct CD8(+) effector and memory populations, which demonstrated unique roles for these inhibitors of E-protein transcription factors. Furthermore, cytokines altered the expression of Id2 and Id3 differently, which provides insight into how external cues influence gene expression.
authors: Division of Biological Sciences, University of California San Diego, La Jolla, California, USA.
authors: Cliff Y
authors: CY
authors: Yang
authors: J Adam
authors: JA
authors: Best
authors: Jamie
authors: J
authors: Knell
authors: Edward
authors: E
authors: Yang
authors: Alison D
authors: AD
authors: Sheridan
authors: Adam K
authors: AK
authors: Jesionek
authors: Haiyan S
authors: HS
authors: Li
authors: Richard R
authors: RR
authors: Rivera
authors: Kristin Camfield
authors: KC
authors: Lind
authors: Louise M
authors: LM
authors: D'Cruz
authors: Stephanie S
authors: SS
authors: Watowich
authors: Cornelis
authors: C
authors: Murre
authors: Ananda W
authors: AW
authors: Goldrath
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D016207:Cytokines / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007156:Immunologic Memory / Q000276:immunology*; D007239:Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics / Q000378:metabolism*; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000378:metabolism*; D037181:Lectins, C-Type; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D011971:Receptors, Immunologic / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D016207:Cytokines; C497452:Idb2 protein, mouse; D051797:Inhibitor of Differentiation Protein 2; D051796:Inhibitor of Differentiation Proteins; C451496:Klrg1 protein, mouse; D037181:Lectins, C-Type; D011971:Receptors, Immunologic; C067679:Idb3 protein, mouse