title: Microbial resistance in relation to catalase activity to oxidative stress induced by photolysis of hydrogen peroxide.
abstract: The purpose of the present study was to evaluate the mechanism of microbial resistance to oxidative stress induced by photolysis of hydrogen peroxide (H(2)O(2)) in relation to microbial catalase activity. In microbicidal tests, Staphylococcus aureus and Candida albicans were killed and this was accompanied by production of hydroxyl radicals. C. albicans was more resistant to hydroxyl radicals generated by photolysis of H(2)O(2) than was S. aureus. A catalase activity assay demonstrated that C. albicans had stronger catalase activity; accordingly, catalase activity could be one of the reasons for the resistance of the fungus to photolysis of H(2)O(2). Indeed, it was demonstrated that C. albicans with strong catalase activity was more resistant to photolysis of H(2)O(2) than that with weak catalase activity. Kinetic analysis using a modified Lineweaver-Burk plot also demonstrated that the microorganisms reacted directly with hydroxyl radicals and that this was accompanied by decomposition of H(2)O(2). The results of the present study suggest that the microbicidal effects of hydroxyl radicals generated by photolysis of H(2)O(2) can be alleviated by decomposition of H(2)O(2) by catalase in microorganisms.
authors: New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan. keisuke@m.tohoku.ac.jp
authors: Keisuke
authors: K
authors: Nakamura
authors: Taro
authors: T
authors: Kanno
authors: Takayuki
authors: T
authors: Mokudai
authors: Atsuo
authors: A
authors: Iwasawa
authors: Yoshimi
authors: Y
authors: Niwano
authors: Masahiro
authors: M
authors: Kohno
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D002374:Catalase / Q000378:metabolism*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology / Q000528:radiation effects; D017665:Hydroxyl Radical / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010782:Photolysis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001426:Bacterial Proteins; D003470:Culture Media; D005656:Fungal Proteins; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D002374:Catalase