title: SAGA and ATAC histone acetyl transferase complexes regulate distinct sets of genes and ATAC defines a class of p300-independent enhancers.
abstract: Histone acetyltransferase (HAT) complexes are coactivators that are important for transcriptional activation by modifying chromatin. Metazoan SAGA and ATAC are distinct multisubunits complexes that share the same catalytic HAT subunit (GCN5 or PCAF). Here, we show that these human HAT complexes are targeted to different genomic loci representing functionally distinct regulatory elements both at broadly expressed and tissue-specific genes. While SAGA can principally be found at promoters, ATAC is recruited to promoters and enhancers, yet only its enhancer binding is cell-type specific. Furthermore, we show that ATAC functions at a set of enhancers that are not bound by p300, revealing a class of enhancers not yet identified. These findings demonstrate important functional differences between SAGA and ATAC coactivator complexes at the level of the genome and define a role for the ATAC complex in the regulation of a set of enhancers.
authors: Program of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, BP 10142, 67404 Illkirch Cedex, CU de Strasbourg, France.
authors: Arnaud R
authors: AR
authors: Krebs
authors: Program of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, BP 10142, 67404 Illkirch Cedex, CU de Strasbourg, France.
authors: Krishanpal
authors: K
authors: Karmodiya
authors: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, C-315240 Longwood Avenue, Boston, MA 02115, USA.
authors: Marianne
authors: M
authors: Lindahl-Allen
authors: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, C-315240 Longwood Avenue, Boston, MA 02115, USA.
authors: Kevin
authors: K
authors: Struhl
authors: Program of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg, BP 10142, 67404 Illkirch Cedex, CU de Strasbourg, France.
authors: Làszlò
authors: L
authors: Tora
mesh_terms: D001665:Binding Sites; D004257:DNA Polymerase II / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D014158:Transcription, Genetic; D014162:Transfection; D050880:p300-CBP Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D004257:DNA Polymerase II; D051548:Histone Acetyltransferases; D046912:Multiprotein Complexes; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C501179:KAT2A protein, human