title: Metabolic cycling without cell division cycling in respiring yeast.
abstract: Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth.
authors: Departments of Biology and Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. nslavov@alum.mit.edu
authors: Nikolai
authors: N
authors: Slavov
authors: Joanna
authors: J
authors: Macinskas
authors: Amy
authors: A
authors: Caudy
authors: David
authors: D
authors: Botstein
mesh_terms: D002455:Cell Division / Q000502:physiology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal / Q000235:genetics*; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D046228:Microarray Analysis; D010101:Oxygen Consumption / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger