title: Enantioselective recognition of mandelic acid based on γ-globulin modified glassy carbon electrode.
abstract: A new chiral biosensor has been fabricated by immobilizing γ-globulin on gold nanoparticles modified glassy carbon electrodes, which could recognize and detect mandelic acid (MA) enantiomers. Differential pulse voltammetry, quartz crystal microbalance, ultraviolet-visible spectroscopy, and atomic force microscopy were used to characterize the enantioselectivity. The results exhibited that γ-globulin modified electrode could enantioselectively recognize MA enantiomers, and larger response signals were obtained from R-MA. The factors influencing the performance of the resulting biosensor were investigated. The enantiomeric composition of R- and S-MA enantiomer mixtures could be determined by measuring the current responses of the sample. The developed electrodes have the advantages of simple preparation, good stability, and rapid detection.
authors: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. fyzc@swu.edu.cn
authors: Yingzi
authors: Y
authors: Fu
authors: Qiao
authors: Q
authors: Chen
authors: Juan
authors: J
authors: Zhou
authors: Qian
authors: Q
authors: Han
authors: Yonghua
authors: Y
authors: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon; D055664:Electrochemical Techniques; D006046:Gold; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D058525:Quartz Crystal Microbalance Techniques; D013053:Spectrophotometry; D013237:Stereoisomerism; D005719:gamma-Globulins
chemical_list: D008333:Mandelic Acids; D005719:gamma-Globulins; D002244:Carbon; D006046:Gold; C037938:mandelic acid