title: Long-term oral administration of glucosamine or chondroitin sulfate reduces destruction of cartilage and up-regulation of MMP-3 mRNA in a model of spontaneous osteoarthritis in Hartley guinea pigs.
abstract: Histological and molecular changes were examined to investigate the effects of long-term administration of glucosamine (GlcN) and chondroitin sulfate (CS) in a model of spontaneous osteoarthritis (OA) in Hartley guinea pigs. Three groups of female 3-week-old Hartley guinea pigs received GlcN, CS, and neither agent, respectively. Five animals in each group were sacrificed at 8, 12, and 18 months of age. At 8 months of age, Hartley guinea pigs had severe degeneration of knee joint cartilage, chondrocyte apoptosis, marked reduction of tissue total RNA, decreases of aggrecan and collagen type 2 mRNAs, and increases in MMP-3 and MMP-8 mRNAs. Long-term administration of GlcN and CS reduced cartilage degeneration at 8 months of age. The marked loss of total RNA and the increase in MMP-3 mRNA were also inhibited by GlcN and CS. Thus, long-term oral administration of GlcN or CS inhibits OA progression, maintains total RNA and down-regulates MMP-3 mRNA in a spontaneous OA model in Hartley guinea pigs.
authors: Department of Orthopaedic Surgery, Nihon University School of Medicine, 30-1, Ohyaguchikami-cho, Itabashi-ku, Tokyo 173-8610, Japan.
authors: Shin
authors: S
authors: Taniguchi
authors: Junnosuke
authors: J
authors: Ryu
authors: Masayuki
authors: M
authors: Seki
authors: Takanobu
authors: T
authors: Sumino
authors: Yasuaki
authors: Y
authors: Tokuhashi
authors: Mariko
authors: M
authors: Esumi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology; D019902:Chondrocytes / Q000187:drug effects*; D002809:Chondroitin Sulfates / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D005944:Glucosamine / Q000008:administration & dosage*; D006168:Guinea Pigs; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D010003:Osteoarthritis / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D002809:Chondroitin Sulfates; D019278:Matrix Metalloproteinase 3; D005944:Glucosamine