title: Simultaneous determination of ten flavonoids from Viscum coloratum grown on different host species and different sources by LC-MS.
abstract: A high-performance liquid chromatographic-mass spectrometric method was developed for the simultaneous determination of 10 flavonoids in Viscum coloratum obtained from different host species and different sources. Viscum coloratum was extracted with 50% methanol. The extracts were separated on a C(18) column with a gradient of 0.1% (v/v) formic acid and methanol. The flavonoids in the extracts were detected by negative electrospray ionization mass spectrometry in selective ion monitoring mode. The calibration curves showed good linearity (r>0.998) within the test ranges (homoeriodictyol: 0.149-8.940 µg/ml, homoeriodictyol-7-O-β-D-glycoside: 0.230-13.80 µg/ml, homoeriodictyol-7-O-β-D-apiose (1→2)-β-D-glycoside: 5.000-300.0 µg/ml, homoeriodictyol-7-O-β-D-apiose (1→5)-β-D-apiose (1→2)-β-D-glycoside: 0.835-125.3 µg/ml, rhamnazin-3-O-β-D-glucoside: 0.064-3.840 µg/ml, rhamnazin-3-O-β-D-(6″-β-hydroxy-β-methyglutaryl)-glucoside: 1.435-86.10 µg/ml, isorhamnetin-3-O-β-D-glucoside: 0.930-55.80 µg/ml, 5-hydroxy-3,7,3'-trimethoxyflavone-4'-O-β-D-glucoside: 0.067-4.020 µg/ml, 5,7,4'-trihydroxy-3,3'-dimethoxyflavone: 0.270-16.20 µg/ml, pachypodol: 0.110-6.600 µg/ml). The limits of quantification were between 0.006-0.720 µg/ml. The assay was reproducible and the overall intra- and inter-day variations were less than 4.6%. The recoveries varied from 93.4 to 103.9% at three different concentration levels. The validation method was used to determine the contents of 10 flavonoids in Viscum coloratum. A one-way analysis of variance was applied to evaluate Viscum coloratum-host-source interactions. Compared with the host species, the sample source had a significant impact on the sample content.
authors: Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors: Yunli
authors: Y
authors: Zhao
authors: Zhiguo
authors: Z
authors: Yu
authors: Ronghua
authors: R
authors: Fan
authors: Xiaoxia
authors: X
authors: Gao
authors: Miao
authors: M
authors: Yu
authors: Hongyan
authors: H
authors: Li
authors: Hongjun
authors: H
authors: Wei
authors: Kaishun
authors: K
authors: Bi
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D028181:Viscum / Q000737:chemistry*
chemical_list: D005419:Flavonoids