title: In vitro evolution of H5N1 avian influenza virus toward human-type receptor specificity.
abstract: Acquisition of α2-6 sialoside receptor specificity by α2-3 specific highly-pathogenic avian influenza viruses (H5N1) is thought to be a prerequisite for efficient transmission in humans. By in vitro selection for binding α2-6 sialosides, we identified four variant viruses with amino acid substitutions in the hemagglutinin (S227N, D187G, E190G, and Q196R) that revealed modestly increased α2-6 and minimally decreased α2-3 binding by glycan array analysis. However, a mutant virus combining Q196R with mutations from previous pandemic viruses (Q226L and G228S) revealed predominantly α2-6 binding. Unlike the wild type H5N1, this mutant virus was transmitted by direct contact in the ferret model although not by airborne respiratory droplets. However, a reassortant virus with the mutant hemagglutinin, a human N2 neuraminidase and internal genes from an H5N1 virus was partially transmitted via respiratory droplets. The complex changes required for airborne transmissibility in ferrets suggest that extensive evolution is needed for H5N1 transmissibility in humans.
authors: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States.
authors: Li-Mei
authors: LM
authors: Chen
authors: Ola
authors: O
authors: Blixt
authors: James
authors: J
authors: Stevens
authors: Aleksandr S
authors: AS
authors: Lipatov
authors: Charles T
authors: CT
authors: Davis
authors: Brian E
authors: BE
authors: Collins
authors: Nancy J
authors: NJ
authors: Cox
authors: James C
authors: JC
authors: Paulson
authors: Ruben O
authors: RO
authors: Donis
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D019143:Evolution, Molecular; D005289:Ferrets; D057809:HEK293 Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D005585:Influenza in Birds / Q000635:transmission / Q000821:virology; D007251:Influenza, Human / Q000635:transmission / Q000821:virology; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011134:Polysaccharides / Q000378:metabolism*; D016865:Reassortant Viruses / Q000235:genetics / Q000502:physiology; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D017423:Sequence Analysis, RNA
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011134:Polysaccharides; D011991:Receptors, Virus; D009439:Neuraminidase