title: On-chip sample preparation by controlled release of antibodies for simple CD4 counting.
abstract: We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4(+) and CD8(+) T-lymphocytes correlates well with results obtained by flow cytometry.
authors: Medical Cell Biophysics, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, PO Box 217, 7500 AE Enschede, The Netherlands. M.Beck@utwente.nl
authors: Markus
authors: M
authors: Beck
authors: Silvia
authors: S
authors: Brockhuis
authors: Niels
authors: N
authors: van der Velde
authors: Christian
authors: C
authors: Breukers
authors: Jan
authors: J
authors: Greve
authors: Leon W M M
authors: LW
authors: Terstappen
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D015703:Antigens, CD / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D001800:Blood Specimen Collection; D018791:CD4 Lymphocyte Count / Q000295:instrumentation* / Q000379:methods*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000166:cytology; D005434:Flow Cytometry / Q000295:instrumentation*; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055550:Protein Stability
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D005456:Fluorescent Dyes