title: Regulation of cotton fiber  elongation by xyloglucan endotransglycosylase/hydrolase genes.
abstract: Ligon  lintless mutant (li1li1) with super-short fibers (5-8 mm in  length) and its wild type (Li1Li1) with normal fibers (30 mm in  length) were used to study the function of xyloglucan  endotransglycosylase/hydrolase (XTH) genes during fiber elongation in cotton.  Wild-type cotton attained the fiber elongation stage earlier (5 days  post-anthesis, DPA), than the Ligon lintless mutant (12 DPA) with a higher  fiber elongation velocity of about 1.76 mm/day. Xyloglucan contents in Ligon  lintless mutant fibers were 5-fold higher than the wild type during 9-15 DPA.  It was also observed that the activity of XTH in wild-type cotton fibers was  about 2-fold higher than that of the Ligon lintless mutant with a peak at 12  DPA. DNA blot analysis indicated that the XTH gene in the Ligon lintless  mutant and its wild type belonged to a multiple allelic series. However, RNA  blot analysis and quantitative real-time PCR exhibited an earlier expression  (10 DPA) of XTH in wild type as compared to delayed (15 DPA) expression  in the Ligon lintless mutant. The study also revealed that 9-15 DPA might be a  key phase for upregulation of fiber elongation via increasing XTH activity.  Higher XTH activity can cleave down the xyloglucan-cellulose chains thus  loosening fiber cell wall and promoting fiber cell elongation in wild type as  compared to its mutant.
authors: Department of Agronomy, Zhejiang University, Hangzhou, PR China.
authors: M Y
authors: MY
authors: Shao
authors: X D
authors: XD
authors: Wang
authors: M
authors: M
authors: Ni
authors: N
authors: N
authors: Bibi
authors: S N
authors: SN
authors: Yuan
authors: W
authors: W
authors: Malik
authors: H P
authors: HP
authors: Zhang
authors: Y X
authors: YX
authors: Liu
authors: S J
authors: SJ
authors: Hua
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D003124:Colorimetry; D032561:Cotton Fiber; D004587:Electrophoresis, Agar Gel; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005936:Glucans / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000033:anatomy & histology; D014990:Xylans / Q000378:metabolism
chemical_list: D005936:Glucans; D018749:RNA, Plant; D014990:Xylans; C029353:xyloglucan; D016695:Glycosyltransferases; C073693:xyloglucan endotransglycosylase