title: Antimicrobial susceptibility testing of German Campylobacter fetus subsp. venerealis isolates by agar disk diffusion method.
abstract: Campylobacter fetus subsp. venerealis is the causative agent of bovine genital campylobacteriosis and is transmitted by asymptomatic carrier bulls via contaminated semen during artificial insemination. The aim of the present study was to determine the in vitro susceptibility of Campylobacter fetus subsp. venerealis isolated from bovine specimens in the years from 2000 to 2009 in Germany to antibiotics generally used in semen treatment. The susceptibilities of 50 strains to spectinomycin (10 microg), gentamicin (10 microg), streptomycin (25 microg), penicillin (10 microg), lincomycin (10 microg), ciprofloxacin (5 microg), erythromycin (30 microg) and tetracycline (30 microg) were determined using a disk diffusion susceptibility test. All strains were susceptible to gentamicin. A considerably reduced susceptibility to one or more antimicrobial agents was detected in seven out of 50 isolates (14%) with the most frequent reduction in susceptibility to lincomycin and spectinomycin. Furthermore, strains with reduced susceptibility to more than one antimicrobial agent were always associated with reduced susceptibility to lincomycin. It is recommended to determine the antimicrobial susceptibility of Campylobacter fetus subsp. venerealis isolates in order to evaluate the efficacy of the generally used antibiotic treatment of bull semen and to detect possible resistances.
authors: Friedrich-Loeffler-Lnstitut, Bundesforschungsinstitut für Tiergesundheit, Institut für bakterielle Infektionen und Zoonosen, Jena, Germany. ingrid.haenel@fli.bund.de
authors: Ingrid
authors: I
authors: Hänel
authors: Helmut
authors: H
authors: Hotzel
authors: Wolfgang
authors: W
authors: Müller
authors: Herbert
authors: H
authors: Tomaso
mesh_terms: D037881:Aborted Fetus / Q000382:microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002168:Campylobacter fetus / Q000187:drug effects* / Q000302:isolation & purification; D002417:Cattle; D052978:Disk Diffusion Antimicrobial Tests / Q000662:veterinary*; D005260:Female; D005858:Germany; D008297:Male; D010413:Penis / Q000382:microbiology; D012661:Semen / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents