title: Metabolic and hematologic consequences of colectomy associated to hepatectomy in rats.
abstract: OBJECTIVE
  To investigate the influence of partial colectomy associated with hepatectomy on the biodistribution of the (99m)Tc-phytate, on metabolic parameters, as well as labeling and morphology of red blood cells.
  
  
  METHODS
  Wistar rats were distributed into three groups (each with six), nominated as colectomy, colectomy+hepatectomy and sham. In the 30(th) postoperative day all rats were injected with (99m)Tc-phytate 0.1mL i.v. (radioactivity 0.66 MBq). After 15 minutes, liver sample was harvested and weighed. Percentage radioactivity per gram of tissue (%ATI/g) was determined using an automatic gamma-counter. Serum AST, ALT, alkaline phosphatase and red blood cells labeling were determined.
  
  
  RESULTS
  The liver %ATI/g and red blood cells labeling were lower in colectomy and colectomy+hepatectomy rats than in sham rats (p <0.05), and no difference was detected comparing the colectomy and colectomy+hepatectomy groups. Red blood cells morphology did not differ among groups. Serum levels of AST, ALT and alkaline fosfatase were significantly higher in colectomy+hepatectomy than in colectomy rats (p<0.001).
  
  
  CONCLUSIONS
  Hepatectomy associated with colectomy lowered the uptake of radiopharmaceutical in liver and in red blood cells in rats, coinciding with changes in liver enzymatic activity.
authors: Postgraduate Program of Health Sciences, UFRN, Natal, Brazil.
authors: Marília Daniela Ferreira
authors: MD
authors: Carvalho
authors: Irami
authors: I
authors: Araújo-Filho
authors: Italo Medeiros
authors: IM
authors: Azevedo
authors: Amália Cínthia Meneses
authors: AC
authors: Rêgo
authors: Aldo Cunha
authors: AC
authors: Medeiros
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D003082:Colectomy; D004912:Erythrocytes / Q000378:metabolism*; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D013670:Sodium Pertechnetate Tc 99m; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase