title: DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance.
abstract: DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic traits.
authors: Guizhou Institute of Tobacco Science, Guiyang 550081, China.
authors: Jie-hong
authors: JH
authors: Zhao
authors: Ji-shun
authors: JS
authors: Zhang
authors: Yi
authors: Y
authors: Wang
authors: Ren-gang
authors: RG
authors: Wang
authors: Chun
authors: C
authors: Wu
authors: Long-jiang
authors: LJ
authors: Fan
authors: Xue-liang
authors: XL
authors: Ren
mesh_terms: D016000:Cluster Analysis; D019175:DNA Methylation; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D044127:Epigenesis, Genetic; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014026:Nicotiana / Q000145:classification / Q000235:genetics* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000502:physiology*
chemical_list: D018744:DNA, Plant