title: [Screening of optimal signal peptide for heterologous xylanase secretion by Bacillus subtilis].
abstract: OBJECTIVE
  We searched optimal signal peptide for heterologous and exogenous secretion of xylanase in Bacillus subtilis.
  
  
  METHODS
  We constructed a screening vector for signal peptides from B. subtilis. The Alkali resistance xylanase gene (xynA) from Bacillus pumilus was chosen as reporter gene and cloned into E. coli and B. subtilis shuttle vector pGJ148 which has maltose-inducible promoter Pglv and spectinomycin resistant gene. 24 Sec-type signal peptides (SPs) was amplified from B. Subtilis 1A747 and cloned into the screening vector for the expression of xynA in B. Subtilis WB700. The xylanase activity of the culture supernatant were detected after 24h incubation.
  
  
  RESULTS
  The screening of these signal peptides revealed differences in xylanase activity of the culture supernatants, The recombinant strain containing YnfF signal peptide showed the highest xylanase acitivity (37.2 IU/mL).
  
  
  CONCLUSIONS
  Experiment proved screening of signal peptides is effective way for optimization of the export of heterologous protein in B. subtilis.
authors: Department of Animal Science, Northwest A&F University, Yangling 712100, China.
authors: Xin
authors: X
authors: Fan
authors: Mingming
authors: M
authors: Yang
authors: Pengtao
authors: P
authors: Cao
authors: Wei
authors: W
authors: Zhang
authors: Wen
authors: W
authors: Zhang
authors: Xiuping
authors: X
authors: Song
authors: Yuesheng
authors: Y
authors: Gong
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases