title: High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
abstract: The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.
authors: Department of Biochemistry, School of Life Science, Fudan University, Shanghai, China.
authors: Yingnan
authors: Y
authors: Bian
authors: Wei
authors: W
authors: Zhou
authors: Yingchun
authors: Y
authors: Zhao
authors: Xiaoping
authors: X
authors: Li
authors: Wei
authors: W
authors: Geng
authors: Ruixin
authors: R
authors: Hao
authors: Qing
authors: Q
authors: Yang
authors: Weida
authors: W
authors: Huang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D016466:CHO Cells; D047369:Chromatin Immunoprecipitation; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D024202:Electrophoretic Mobility Shift Assay; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases; D051379:Mice; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000502:physiology; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; C507007:Eri1 protein, mouse; D005092:Exonucleases; D005095:Exoribonucleases