title: Overexpression of the Trichoderma brevicompactum tri5 gene: effect on the expression of the trichodermin biosynthetic genes and on tomato seedlings.
abstract: Trichoderma brevicompactum IBT 40841 produces trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
authors: Spanish-Portuguese Center of Agricultural Research (CIALE), Department of Microbiology and Genetics, University of Salamanca, Campus of Villamayor, Río Duero 12, 37185 Salamanca, Spain. atijerino@usal.es
authors: Anamariela
authors: A
authors: Tijerino
authors: Rosa
authors: R
authors: Hermosa
authors: Rosa E
authors: RE
authors: Cardoza
authors: Javier
authors: J
authors: Moraga
authors: Monica G
authors: MG
authors: Malmierca
authors: Josefina
authors: J
authors: Aleu
authors: Isidro G
authors: IG
authors: Collado
authors: Enrique
authors: E
authors: Monte
authors: Santiago
authors: S
authors: Gutierrez
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology; D014242:Trichoderma / Q000235:genetics*; D014243:Trichodermin / Q000378:metabolism* / Q000633:toxicity
chemical_list: D000975:Antioxidants; C011867:4-hydroxyphenylethanol; D014243:Trichodermin; D010626:Phenylethyl Alcohol