title: Efficacy of cream-based novel formulations of hyaluronic acid of different molecular weights in anti-wrinkle treatment.
abstract: BACKGROUND
  Due to its strong water-binding potential, hyaluronic acid (HA) is a well-known active ingredient for cosmetic applications. Native HA is proposed to help the skin to retain and maintain elasticity, turgor and moisture.
  
  
  OBJECTIVE
  To observe the efficacy of topical application of 0.1% hyaluronan formulations of different molecular weights (MW) (50, 130, 300, 800 and 2000 kDa, respectively) in the periocular area as anti-wrinkle treatment.
  
  
  METHODS
  Seventy-six female subjects between 30 and 60 years of age with clinical signs of periocular wrinkles applied one of the formulations twice-daily to the area of interest in a randomized fashion for 60 days. Around the other eye, a vehicle control cream was applied. Measurements of skin hydration and skin elasticity were performed before treatment, 30 and 60 days thereafter. At similar time points negative replicas were taken and evaluated by semi-automated morphometry.
  
  
  RESULTS
  All HA-based creams utilized in this study demonstrated a significant improvement in skin hydration and overall elasticity values (R2) when compared to placebo. Measurements of wrinkle depth using mean roughness (Ra) and maximum roughness (Rz) values revealed significant improvement in the 130 and the 50 kDa HA group after 60 days of treatment compared to placebo-treated area.
  
  
  CONCLUSIONS
  Topical application of all 0.1% HA formulations used in this study led to significant improvement in skin hydration and elasticity. Application of low-molecular-weight (LMW) HA was associated with significant reduction of wrinkle depth, which may be due to better penetration abilities of LMW HA.
authors: Department of Dermatology and Allergology, Ludwig Maximilians University, Munich, Germany. tatjana.pavicic@med.uni-muenchen.de
authors: Tatjana
authors: T
authors: Pavicic
authors: Gerd G
authors: GG
authors: Gauglitz
authors: Peter
authors: P
authors: Lersch
authors: Khadija
authors: K
authors: Schwach-Abdellaoui
authors: Birgitte
authors: B
authors: Malle
authors: Hans Christian
authors: HC
authors: Korting
authors: Mike
authors: M
authors: Farwick
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D003357:Cosmetic Techniques; D004548:Elasticity / Q000187:drug effects; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D008875:Middle Aged; D008970:Molecular Weight; D010539:Permeability; D012869:Skin Absorption; D015595:Skin Aging / Q000187:drug effects*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D006820:Hyaluronic Acid