title: Development and evaluation of new primers for PCR-based  identification of type II fimA of Porphyromonas gingivalis.
abstract: For more accurate PCR-based identification of Porphyromonas gingivalis harboring genotype II fimA, the most prevalent type in periodontitis patients, a new primer set was developed and evaluated. The previous type II primers hybridized to the DNA of P gingivalis strains harboring type Ib as well as type II fimA, while the new primers specifically amplified only the DNA fragment of type II fimA. In the investigation using mixed bacterial culture and 155 clinical samples from peri-implantitis patients, the new primers increased the accuracy of PCR-based detection of type II fimA by excluding false-negatives as well as false-positives.
authors: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea; 2Institute of Oral Biology, Kyung Hee University, Seoul, Korea.
authors: Ji-Hoi
authors: JH
authors: Moon
authors: Seung-Il
authors: SI
authors: Shin
authors: Jong-Hyuk
authors: JH
authors: Chung
authors: Seung-Woo
authors: SW
authors: Lee
authors: Atsuo
authors: A
authors: Amano
authors: Jin-Yong
authors: JY
authors: Lee
mesh_terms: D016866:Bacteroidaceae Infections / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D005838:Genotype; D058889:Molecular Typing / Q000379:methods*; D010518:Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; C063055:fimbrillin; D036781:Fimbriae Proteins