title: Magnetic field-induced acceleration of the accumulation of magnetic iron oxide nanoparticles by cultured brain astrocytes.
abstract: Magnetic iron oxide nanoparticles (Fe-NPs) are considered for various biomedical and neurobiological applications that involve the presence of external magnetic fields. However, little is known on the effects of a magnetic field on the uptake of such particles by brain cells. Cultured brain astrocytes accumulated dimercaptosuccinate-coated Fe-NP in a time-, temperature-, and concentration-dependent manner. This accumulation was strongly enhanced by the presence of the magnetic field generated by a permanent neodymium iron boron magnet that had been positioned below the cells. The magnetic field-induced acceleration of the accumulation of Fe-NP increased almost proportional to the strength of the magnetic field applied, increasing the cellular-specific iron content from an initial 10 nmol/mg protein within 4 h of incubation at 37°C to up to 12,000 nmol/mg protein. However, presence of a magnetic field also increased the amounts of iron that attached to the cells during incubation with Fe-NP at 4°C. These results suggest that the presence of an external magnetic field promotes in cultured astrocytes both the binding of Fe-NP to the cell membrane and the internalization of Fe-NP.
authors: Centre for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany.
authors: Marie-Christin
authors: MC
authors: Lamkowsky
authors: Mark
authors: M
authors: Geppert
authors: Maike M
authors: MM
authors: Schmidt
authors: Ralf
authors: R
authors: Dringen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D004705:Endocytosis; D007501:Iron / Q000378:metabolism; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017208:Rats, Wistar; D013696:Temperature; D013997:Time Factors
chemical_list: D058185:Magnetite Nanoparticles; D007501:Iron