title: Surveillance of adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995-2010.
abstract: Human adenoviruses species D (HAdV-D) are known to cause severe epidemic keratoconjunctivitis. However, the isolation rate of HAdV-D is not high, because HAdV-D is usually slow to propagate. Although new types of HAdV-D have been reported, accurate surveillance has not been performed because of difficulties in culturing the viruses and lack of a practical identification method. In this study, HAdV-Ds were detected and identified from patients with epidemic keratoconjunctivitis in the Fukui Prefecture during 1995-2010 by PCR, loop-mediated isothermal amplification (LAMP) of DNA, and conventional virus isolation and neutralization tests. All samples were subjected to culture and PCR and LAMP. A total of 124 strains of HAdV-D were detected from 157 patients with epidemic keratoconjunctivitis. The strains consisted of the following types: D8 (n = 8), D19 (n = 4), D37 (n = 40), D53 (n = 5), D54 (n = 66), and D56 (n = 1). Among these, D53, D54, and D56 are new types that have been reported recently. The results of this study demonstrated that new types of HAdV-D caused epidemic keratoconjunctivitis during 1995-2010, and included an outbreak of keratoconjunctivitis caused by HAdV-D54. The LAMP method was able to detect and identify HAdV-D53 and HAdV-D54 in 1 hr, and may therefore be applicable for use at the bedside.
authors: Fukui Prefectural Institute of Public Health and Environmental Science, Fukui, Japan.
authors: Masako
authors: M
authors: Nakamura
authors: Eiko
authors: E
authors: Hirano
authors: Kazuaki
authors: K
authors: Kowada
authors: Fubito
authors: F
authors: Ishiguro
authors: Zenya
authors: Z
authors: Yamagishi
authors: Arun Kumar
authors: AK
authors: Adhikary
authors: Nozomu
authors: N
authors: Hanaoka
authors: Nobuhiko
authors: N
authors: Okabe
authors: Kiyosu
authors: K
authors: Taniguchi
authors: Tsuguto
authors: T
authors: Fujimoto
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology* / Q000821:virology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D021141:Nucleic Acid Amplification Techniques; D017422:Sequence Analysis, DNA; D014776:Virus Cultivation