title: Iron oxide nanoparticle-induced epidermal growth factor receptor expression in human stem cells for tumor therapy.
abstract: Superparamagnetic iron oxide (SPIO) nanoparticles show promise as labels for cellular magnetic resonance imaging (MRI) in the application of stem cell-based therapy. However, the unaddressed concerns about the impact of SPIO nanoparticles on stem cell attributes make the feasibility of SPIO labeling uncertain. Here, we show that the labeling of human mesenchymal stem cells (hMSCs) with ferucarbotran can induce epidermal growth factor receptor (EGFR) overexpression. Labeled hMSCs with their overexpressed EGFR were attracted by tumorous EGF and more effectively migrated toward tumor than unlabeled cells, resulting in more potent intrinsic antitumor activity. Moreover, the captured binding of tumorous EGF by overexpressed EGFR of labeled hMSCs blocked EGF/EGFR signaling-derived tumor growth, tumorous angiogenesis, and tumorous VEGF expression also responsible for tumor progression and development. Our results show that the impact of SPIO nanoparticles on stem cell attributes is not necessarily harmful but can be cleverly used to be beneficial to stem cell-based therapy.
authors: Center for Nanomedicine Research, National Health Research Institutes, Miaoli, Taiwan, Republic of China.
authors: Tsai-Hua
authors: TH
authors: Chung
authors: Jong-Kai
authors: JK
authors: Hsiao
authors: Szu-Chun
authors: SC
authors: Hsu
authors: Ming
authors: M
authors: Yao
authors: Yao-Chang
authors: YC
authors: Chen
authors: Shih-Wei
authors: SW
authors: Wang
authors: Mark Yen-Ping
authors: MY
authors: Kuo
authors: Chung-Shi
authors: CS
authors: Yang
authors: Dong-Ming
authors: DM
authors: Huang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000628:therapy*; D003131:Combined Modality Therapy; D003287:Contrast Media / Q000008:administration & dosage / Q000009:adverse effects; D003911:Dextrans / Q000008:administration & dosage* / Q000009:adverse effects; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000009:adverse effects; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000009:adverse effects; D016896:Treatment Outcome
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; C512478:EGFR protein, human; D066246:ErbB Receptors