title: Copper(II) chloride mediated synthesis and DNA photocleavage activity of 1-aryl/heteroaryl-4-substituted-1,2,4-triazolo[4,3-a]quinoxalines.
abstract: A new class of photonucleases, 1-aryl/heteroaryl-4-substituted-1,2,4-triazolo[4,3-a]quinoxalines (4) was synthesized in a facile and efficient manner via copper(II) chloride mediated oxidative intramolecular cyclization of 2-(arylidenehydrazino)-3-substituted-quinoxalines (3). DNA cleavage potency of compounds 4a-d (40 μg each) was quantitatively evaluated on supercoiled plasmid ΦX174 under UV irradiation (312 nm, 15 W) without any additive. Compound 4c was found to be the most efficient DNA photocleaver which had converted supercoiled DNA (form I) into the relaxed DNA (form II) at 30 μg and the DNA photocleavage activity increases with increase in concentration of 4c.
authors: Department of Chemistry, Kurukshetra University, Kurukshetra 136 119, India. ranjana67in@yahoo.com
authors: Ranjana
authors: R
authors: Aggarwal
authors: Garima
authors: G
authors: Sumran
authors: Virender
authors: V
authors: Kumar
authors: Ashwani
authors: A
authors: Mittal
mesh_terms: D003300:Copper / Q000737:chemistry*; D003500:Cyclization; D053837:DNA Cleavage / Q000187:drug effects*; D004278:DNA, Superhelical / Q000378:metabolism*; D010084:Oxidation-Reduction; D010782:Photolysis / Q000187:drug effects*; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D011810:Quinoxalines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D004278:DNA, Superhelical; D011810:Quinoxalines; D014230:Triazoles; C045575:1,2,4-triazole; D003300:Copper; C029892:cupric chloride