title: Mass spectrometric measurements of the apolipoproteins of bovine (Bos taurus) HDL.
abstract: As is the case in most mammals, high density lipoproteins (HDL) also comprise the major group of lipid carriers that circulate in bovine (Bos taurus) blood. As a continuation of our proteogenomic studies of mammalian apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with bovine HDL. The major apolipoprotein on the HDL surface is apoA-I, but other apolipoproteins were also detected. Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I, proA-I and A-II, as well as post-translationally modified apoA-I. Analyses of tryptic fragments did reveal the presence of apoA-IV and apoC-III. However, in contrast to our previous studies of other mammalian HDL, we did not detect apoC-I. Interestingly, examination of the current assembly for the bovine genome does not show any evidence for an apoC-I gene.
authors: Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Italy.
authors: Lorenza
authors: L
authors: Della Donna
authors: Sara
authors: S
authors: Bassilian
authors: Puneet
authors: P
authors: Souda
authors: Carlo
authors: C
authors: Nebbia
authors: Julian P
authors: JP
authors: Whitelegge
authors: Donald L
authors: DL
authors: Puppione
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism; D053305:Apolipoprotein C-III / Q000737:chemistry / Q000378:metabolism; D001053:Apolipoproteins / Q000737:chemistry / Q000378:metabolism*; D001054:Apolipoproteins A / Q000737:chemistry / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D016632:Apolipoprotein A-I; D053305:Apolipoprotein C-III; D001053:Apolipoproteins; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL; C022205:apolipoprotein A-IV