title: [Isolation and characterization of promoter of ADS from Artemisia annua].
abstract: OBJECTIVE
  To try to find the ways to enhance the expression of ADS gene encoding amorpha-4,11-diene synthase, a key enzyme in artemisinin biosynthesis pathway catalyzing the formation of amorpha-4,11-diene from farnesyl diphosphate, and accelerate the artemisinin synthesis, the promoter of ADS was isolated and characterized.
  
  
  METHODS
  5' untranslated regions of ADS were isolated from Artemisia annua with PCR. For functional characterization, the isolated fragment was fused with GUS reporter gene and introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. The GUS expression regulated by 5' untranslated regions of ADS in transgenic N. tabacum under the normal or stressed conditions were detected by histochemical staining and quantitative spectrophotometry assay.
  
  
  RESULTS
  The 2 448 bp DNA fragment upstream of ADS coding sequence was isolated from A. annua and introduced into N. tabacum. Histochemical staining showed that the isolated fragment conferred stable GUS expression in transgenic plants. The quantitative results showed that the GUS activity in transgenic tobacco plants treated by low-temperature (4 degrees C) and ultraviolet irradiation were 1. 6 and 2.2 folds higher than that in the controls.
  
  
  CONCLUSIONS
  It was suggested that the isolated fragment had promoter activity and maybe responsive to adverse environmental stresses.
authors: Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. rysyry@gzhtcm.edu.cn
authors: Ruiyi
authors: R
authors: Yang
authors: Xueqin
authors: X
authors: Yang
authors: Liling
authors: L
authors: Feng
authors: Qingping
authors: Q
authors: Zeng
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D031172:Artemisia annua / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D019883:Alkyl and Aryl Transferases; C403714:amorpha-4,11-diene synthase