title: Detection and analysis of proteins modified by O-linked N-acetylglucosamine.
abstract: O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that is implicated in the etiology of type II diabetes and Alzheimer's disease, as well as cardioprotection. This unit covers simple and comprehensive techniques for identifying proteins modified by O-GlcNAc, studying the enzymes that add and remove O-GlcNAc, and mapping O-GlcNAc modification sites.
authors: The Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors: Natasha E
authors: NE
authors: Zachara
authors: Drexel University College of Medicine, Philadelphia, Pennsylvania.
authors: Keith
authors: K
authors: Vosseller
authors: The Johns Hopkins University School of Medicine, Baltimore, Maryland.
authors: Gerald W
authors: GW
authors: Hart
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000906:Antibodies / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D005700:Galactosyltransferases / Q000378:metabolism; D006031:Glycosylation; D006596:Hexosaminidases / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000906:Antibodies; D011506:Proteins; D005700:Galactosyltransferases; D006596:Hexosaminidases; D000117:Acetylglucosamine