title: Identification of novel virus inhibitors by influenza A virus specific reporter cell based screening.
abstract: As influenza viruses have developed resistance towards current drugs, it is urgent to find potential novel antiviral inhibitors. Here we generated an influenza virus reporter cell line in which the luciferase gene was driven by the influenza virus promoter and screened a small compound library (NCI Diversity Set II). Ten compounds were identified to have inhibitory activity against influenza A virus H1N1. Among them, four compounds blocked influenza virus replication through inhibiting the activity of vRNP. The compound NSC 335506 inhibited HA-mediated membrane fusion. It showed the inhibitory activity against H1N1, H9N2 and H5N1 subtype but not H3N2. Our results demonstrated that influenza virus reporter cell is a very useful tool to identify novel inhibitors against influenza A virus.
authors: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Junjie
authors: J
authors: Zhang
authors: Ting
authors: T
authors: Liu
authors: Xiaomei
authors: X
authors: Tong
authors: Gang
authors: G
authors: Li
authors: Jinghua
authors: J
authors: Yan
authors: Xin
authors: X
authors: Ye
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004285:Dogs; D017930:Genes, Reporter; D057809:HEK293 Cells; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D054852:Small Molecule Libraries; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D054852:Small Molecule Libraries