title: [Correlation of DNJ between taxilli herba and its host-plants].
abstract: OBJECTIVE
  To study the correlation of 1-deoxynojirimycin (DNJ) between Taxilli Herba parasitized in mulberry and its host-plants.
  
  
  METHODS
  The contents of DNJ of Taxilli Herba parasitized in mulberry and non-mulberry were determined by RP-HPLC. DNJ was extracted with 0.05 mol x L(-1) HCl, and then detected by fluorescence detector after derivatized with FMOC-Cl at pH 8.0 with borate buffer. The separation was performed on an Agilent C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase of acetonitrile-0.1% aqueous acetic acid (51: 49) at a flow rate of 1.0 mL x min(-1). The wavelength of fluorescence detector was operated at lambda(EX) = 254 nm and lambda(EM) = 322 nm.
  
  
  RESULTS
  The linear range of DNJ was 3.72-37.2 mg x L(-1) (r = 0.999 9). The average recovery was 96.42%. The contents of DNJ in mulberry and Taxilli Herba parasitized in mulberry were 1.39-10.16 mg x g(-1) and 0.46-2.72 mg x g(-1), respectively. However, the contents of DNJ could not be detected in Taxilli Herba parasitized in non mulberry and its host-plants.
  
  
  CONCLUSIONS
  As the characteristic constituent of mulberry, DNJ was accumulated in Taxilli Herba This method can be applied to the quality control of Taxilli Herba from mulberry.
authors: Academy of Traditional Medicine and Pharmacy of Qinzhou, Qinzhou 535000, China. liyonghua185@126.com
authors: Yonghua
authors: Y
authors: Li
authors: Benwei
authors: B
authors: Su
authors: Xiejun
authors: X
authors: Zhang
authors: Kaixin
authors: K
authors: Zhu
authors: Hehuan
authors: H
authors: Pei
authors: Minghui
authors: M
authors: Zhao
authors: Dong
authors: D
authors: Lu
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D031625:Morus / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D017485:1-Deoxynojirimycin