title: Anti-inflammatory phenolics isolated from Juniperus rigida leaves and twigs in lipopolysaccharide-stimulated RAW264.7 macrophage cells.
abstract: Inflammation is an essential host defense system particularly in response to infection and injury; however, excessive or undesirable inflammatory responses contribute to acute and chronic human diseases. A high-throughput screening effort searching for anti-inflammatory compounds from medicinal plants deduced that the methanolic extract of Juniperus rigida S. et L. (Cupressaceae) inhibited significantly nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Activity-guided fractionation and isolation yielded 13 phenolic compounds, including one new phenylpropanoid glycosides, 3,4-dimethoxycinnamyl 9-O-β-D-glucopyranoside (1). Among the isolated compounds, phenylpropanoid glycosides with p-hydroxy group (2, 4) and massoniaside A (7), (+)-catechin (10), amentoflavone (11) effectively inhibited LPS-induced NO production in RAW264.7 cells.
authors: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors: Eun Ju
authors: EJ
authors: Jeong
authors: Hanee
authors: H
authors: Seo
authors: Heejung
authors: H
authors: Yang
authors: Jinwoong
authors: J
authors: Kim
authors: Sang Hyun
authors: SH
authors: Sung
authors: Young Choong
authors: YC
authors: Kim
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology; D044946:Biflavonoids / Q000302:isolation & purification / Q000494:pharmacology; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D018653:Juniperus / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D000432:Methanol; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents
chemical_list: C484708:3,4,9'-trihydroxy-3-methoxy-7,8-dihydrobenzofuran-1'-propanolneoligan-9-O-rhamnopyranoside; C581416:3,4-dimethoxycinnamyl 9-O-beta-D-glucopyranoside; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001572:Benzofurans; D044946:Biflavonoids; D002934:Cinnamates; D005960:Glucosides; D006027:Glycosides; D008070:Lipopolysaccharides; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D009569:Nitric Oxide; D002392:Catechin; C011164:amentoflavone; D000432:Methanol