title: Manassantin A isolated from Saururus chinensis inhibits 5-lipoxygenase-dependent leukotriene C4 generation by blocking mitogen-activated protein kinase activation in mast cells.
abstract: In this study, manassantin A (Man A), an herbal medicine isolated from Saururus chinensis (S. chinensis), markedly inhibited 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generation in bone marrow-derived mast cells (BMMCs) in a concentration-dependent manner. To investigate the molecular mechanisms underlying the inhibition of LTC(4) generation by Man A, we assessed the effects of Man A on phosphorylation of cytosolic phospholipase A(2) (cPLA(2)) and mitogen-activated protein kinases (MAPKs). Inhibition of LTC(4) generation by Man A was accompanied by a decrease in cPLA(2) phosphorylation, which occurred via the MAPKs including extracellular signal-regulated protein kinase-1/2 (ERK1/2) as well as p38 and c-Jun N-terminal kinase (JNK) pathways. Taken together, the present study suggests the Man A represents a potential therapeutic approach for the treatment of airway allergic-inflammatory diseases.
authors: College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea.
authors: Su Jeong
authors: SJ
authors: Kim
authors: Yue
authors: Y
authors: Lu
authors: Okyun
authors: O
authors: Kwon
authors: Kyoung
authors: K
authors: Hwangbo
authors: Chang-Seob
authors: CS
authors: Seo
authors: Seung Ho
authors: SH
authors: Lee
authors: Cheorl-Ho
authors: CH
authors: Kim
authors: Young-Chae
authors: YC
authors: Chang
authors: Jong Keun
authors: JK
authors: Son
authors: Hyeun Wook
authors: HW
authors: Chang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001853:Bone Marrow; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D017997:Leukotriene C4 / Q000096:biosynthesis*; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2 / Q000378:metabolism; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D032134:Saururaceae / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D017705:Lignans; D010936:Plant Extracts; D017997:Leukotriene C4; C054895:manassantin A; D001094:Arachidonate 5-Lipoxygenase; D020928:Mitogen-Activated Protein Kinases; D054467:Phospholipases A2