title: A carbon nanoparticle-based low-background biosensing platform for sensitive and label-free fluorescent assay of DNA methylation.
abstract: Combining a DNA intercalator, SYBR Green I, and enzyme-linkage reactions with carbon nanoparticles, a low-background biosensing platform for label-free and sensitive fluorescent assay of DNA methylation is reported.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
authors: Xiangyuan
authors: X
authors: Ouyang
authors: Jinhua
authors: J
authors: Liu
authors: Jishan
authors: J
authors: Li
authors: Ronghua
authors: R
authors: Yang
mesh_terms: D001483:Base Sequence; D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D019175:DNA Methylation; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D009930:Organic Chemicals / Q000737:chemistry; D011804:Quinolines; D013050:Spectrometry, Fluorescence
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D002244:Carbon; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases