title: Inhibition of cortactin and SIRT1 expression attenuates migration and invasion of prostate cancer DU145 cells.
abstract: OBJECTIVE
  Cortactin is overexpressed in various types of cancer and enhances cell motility. It has been recently reported that silent mating type information regulation 2 homolog 1 interacts with cortactin and promotes cell migration. Here, we examined the role of cortactin and silent mating type information regulation 2 homolog 1 in migration and invasion of prostate cancer cells.
  
  
  METHODS
  The cortactin expression levels in DU145, LNCaP and PC3 prostate cancer cells, and in PrEC normal human prostate epithelial cells were evaluated by western blot analysis. In DU145 cells, the expression of cortactin or silent mating type information regulation 2 homolog 1 was inhibited by small interfering RNA, and the effects of their knockdown on migration and invasion were examined by cell migration and invasion assays. To determine the localization of cortactin and silent mating type information regulation 2 homolog 1, western blot and immunofluorescence microscopic analyses were carried out. The functional interaction between silent mating type information regulation 2 homolog 1 and cortactin was also studied by in vivo acetylation assay.
  
  
  RESULTS
  The protein expression of cortactin was significantly higher in DU145 cells than in other cell lines. Knockdown of cortactin or silent mating type information regulation 2 homolog 1 expression inhibited both migration and invasion of DU145 cells. Similarly to cortactin, silent mating type information regulation 2 homolog 1 was found to be predominantly expressed in the cytoplasm. Finally, the knockdown of silent mating type information regulation 2 homolog 1 expression increased the acetylation level of cortactin.
  
  
  CONCLUSIONS
  Our findings suggest that inhibition of cortactin or silent mating type information regulation 2 homolog 1 expression attenuates migration and invasion of DU145 cells and this could represent a promising strategy to regulate metastasis of prostate cancer.
authors: Department of Urology, Gifu University Graduate School of Medicine, Gifu, Japan.
authors: Keita
authors: K
authors: Nakane
authors: Yasunori
authors: Y
authors: Fujita
authors: Riyako
authors: R
authors: Terazawa
authors: Yuko
authors: Y
authors: Atsumi
authors: Taku
authors: T
authors: Kato
authors: Yoshinori
authors: Y
authors: Nozawa
authors: Takashi
authors: T
authors: Deguchi
authors: Masafumi
authors: M
authors: Ito
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D051356:Cortactin / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
chemical_list: C495868:CTTN protein, human; D051356:Cortactin; D034741:RNA, Small Interfering; C447939:SIRT1 protein, human; D056564:Sirtuin 1