title: Z-disc digestion of isolated bovine myofibrils by an endogenous calcium activated neutral proteinase.
abstract: Calcium activated neutral proteinase (CANP) activity has been purified from bovine M. semimembranosus. The effect of the enzyme on purified fractions of beef myofibrils is described. The Z-discs were the main target of the enzyme; small holes developed and the Z-discs were fragmented into smaller units of equal size. The Z-discs fragments were also split transversely, leaving half to each of the neighbouring sarcomeres. Prolonged digestion removed the entire Z-discs and its set of thin myofilaments. The functional importance of the proteinase in meat tenderization is discussed.
authors: Norwegian Food Research Institute, PO Box 50, N-1432 Ås-NLH, Norway.
authors: E
authors: E
authors: Slinde
authors: H
authors: H
authors: Kryvi