title: Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
abstract: The effect of rbGM-CSF intramammary infusion on the subclinical mastitis was evaluated by the somatic cell count (SCC) and expression of adhesion molecules (CD62L and CD11b) on the surface of neutrophils (PMN) in blood and milk. Fifteen cows diagnosed to have subclinical mastitis were used in this study. Seven cows showed a decrease in the SCC (decreased group), whereas 8 cows showed an increase in the SCC (increased group) 7 days after infusion of rbGM-CSF compared to pre infusion level. The percentage of CD62+ cells tended to be lower and CD11b+cells tended to be higher at 6 h on blood PMN in the decreased group of cows. Increased group of cows showed opposite tendencies. The mean fluorescent intensity of these adhesion molecules expressed on PMN in blood and milk was similar in both groups. These results suggested some association between expression of adhesion molecules and changes in SCC by rbGM-CSF. Responsiveness of PMN adhesion molecules to rbGM-CSF might determine the changes in SCC of the subclinical mastitic cows after infusion of rbGM-CSF.
authors: National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kan-nondai, Tsukuba, Ibaraki 305-0856, Japan.
authors: Tomomi
authors: T
authors: Ozawa
authors: Yoshio
authors: Y
authors: Kiku
authors: Megumi
authors: M
authors: Mizuno
authors: Shigeki
authors: S
authors: Inumaru
authors: Shiro
authors: S
authors: Kushibiki
authors: Hiroyuki
authors: H
authors: Shingu
authors: Tomoko
authors: T
authors: Matsubara
authors: Hideyuki
authors: H
authors: Takahashi
authors: Tomohito
authors: T
authors: Hayashi
mesh_terms: D000818:Animals; D058345:Asymptomatic Infections; D001773:Blood Cells / Q000166:cytology; D039481:CD11b Antigen / Q000032:analysis / Q000276:immunology; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D015169:Colony Count, Microbial / Q000662:veterinary; D003612:Dairying; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D007263:Infusions, Parenteral / Q000662:veterinary; D019041:L-Selectin / Q000032:analysis / Q000276:immunology; D008414:Mastitis, Bovine / Q000188:drug therapy* / Q000382:microbiology; D008892:Milk / Q000166:cytology / Q000382:microbiology; D009504:Neutrophils / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use
chemical_list: D039481:CD11b Antigen; D011994:Recombinant Proteins; D019041:L-Selectin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor