title: Fatty acid profile and influence of oxythiamine on fatty acid content in Malassezia pachydermatis, Candida albicans and Saccharomyces cerevisiae.
abstract: Malassezia pachydermatis and Candida albicans are fungi involved in the skin diseases and systemic infections. The therapy of such infections is difficult due to relapses and problems with pathogen identification. In our study, we compare the fatty acids profile of M. pachydermatis, C. albicans and S. cerevisiae to identify diagnostic markers and to investigate the effect of oxythiamine (OT) on the lipid composition of these species. Total fatty acid content is threefold higher in C. albicans and M. pachydermatis compared with S. cerevisiae. These two species have also increased level of polyunsaturated fatty acids (PUFA) and decreased content of monounsaturated fatty acids (MUFA). We noted differences in the content of longer chain (>18) fatty acids between studied species (for example a lack of 20 : 1 in S. cerevisiae and 22 : 0 in M. pachydermatis and C. albicans). OT reduces total fatty acids content in M. pachydermatis by 50%. In S. cerevisiae, OT increased PUFA whereas it decreased MUFA content. In C. albicans, OT decreased PUFA and increased MUFA and SFA content. The results show that the MUFA to PUFA ratio and the fatty acid profile could be useful diagnostic tests to distinguish C. albicans, M. pachydermatis and S. cerevisiae, and OT affected the lipid metabolism of the investigated species, especially M. pachydermatis.
authors: Departament of Cytobiochemistry, Institute of Biology, University of Bialystok, Bialystok, Poland. atyl@uwb.edu.pl
authors: Adam
authors: A
authors: Tylicki
authors: Magdalena
authors: M
authors: Siemieniuk
authors: Pawel
authors: P
authors: Dobrzyn
authors: Grazyna
authors: G
authors: Ziolkowska
authors: Magdalena
authors: M
authors: Nowik
authors: Urszula
authors: U
authors: Czyzewska
authors: Aleksandra
authors: A
authors: Pyrkowska
mesh_terms: D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D003881:Dermatomycoses / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008290:Malassezia / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D010119:Oxythiamine / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010119:Oxythiamine