title: Influence of aerobic and anoxic microenvironments on polyhydroxyalkanoates (PHA) production from food waste and acidogenic effluents using aerobic consortia.
abstract: The functional role of aerobic and anoxic microenvironments on polyhydroxyalkanoates (PHA) production using food waste (UFW) and effluents from acidogenic biohydrogen production process (FFW) were studied employing aerobic mixed culture as biocatalyst. Anoxic microenvironment documented higher PHA production, while aerobic microenvironment showed higher substrate degradation. FFW showed higher PHA accumulation (39.6%) than UFW (35.6%) due to ready availability of precursors (fatty acids). Higher fraction of poly-3-hydroxy butyrate (PHB) was observed compared to poly-3-hydroxy valerate (PHV) in the accumulated PHA in the form of co-polymer [P3(HB-co-HV)]. Dehydrogenase, phosphatase and protease enzymatic activities were monitored during process operation. Integration with fermentative biohydrogen production yielded additional substrate degradation under both aerobic (78%) and anoxic (72%) microenvironments apart from PHA production. Microbial community analysis documented the presence of aerobic and facultative organisms capable of producing PHA. Integration strategy showed feasibility of producing hydrogen along with PHA by consuming fatty acids generated during acidogenic process in association with increased treatment efficiency.
authors: Bioengineering and Environmental Centre (BEEC), Indian Institute of Chemical Technology (IICT), Hyderabad 500 607, India.
authors: M Venkateswar
authors: MV
authors: Reddy
authors: S Venkata
authors: SV
authors: Mohan
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D056804:Biofuels / Q000032:analysis; D057919:Biological Oxygen Demand Analysis; D002241:Carbohydrates / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D005502:Food; D014644:Genetic Variation / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011506:Proteins / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D014865:Waste Disposal, Fluid; D014866:Waste Products / Q000032:analysis*; D018508:Water Purification
chemical_list: D000143:Acids; D056804:Biofuels; D002241:Carbohydrates; D004275:DNA, Ribosomal; D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D011506:Proteins; D014866:Waste Products; D006859:Hydrogen