title: A novel fluorescent probe for NAD-consuming enzymes.
abstract: A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.
authors: School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
authors: Giulia
authors: G
authors: Pergolizzi
authors: Julea N
authors: JN
authors: Butt
authors: Richard P
authors: RP
authors: Bowater
authors: Gerd K
authors: GK
authors: Wagner
mesh_terms: D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D009243:NAD