title: [Biological effects of low dose X-irradiation on human bone marrow mesenchymal stem cells].
abstract: Recent studies have shown that low dose X-irradiation shows specific effect different from high dose exposures. However, the biologic responses of bone marrow mesenchymal stem cells (BM-MSC) to low dose X-irradiation have rarely been described in the literature. This study was purposed to investigate the biologic responses of human bone marrow-derived MSC to low dose X-irradiation. The proliferation of cells was tested with MTT assay, so that the cell growth curve was drawn at 1 to 7 days. The expression of survivin mRNA was detected by RT-PCR assay; the BM-MSC DNA damage induced by X-irradiation were detected with mononuclear cell gel electrophoresis. The results indicated that the proliferative ability of BM-MSC exposed to low doses of X rays was obviously enhanced as compared with control group. The low dose X-irradiation caused the damage of DNA in X-ray dose dependent manner. X-irradiation enhanced expression of survivin in MSC. It is concluded that the low dose below 20 cGy of X-irradiation has a promoting effect on survivin expression in BM-MSC. Whether the high expression of survivin plays an important role to resist ionizing radiation needs to be further studied.
authors: Institute of Hematology, Lanzhou Military Area General Hospital, Lanzhou, Gansu Province, China.
authors: Bing
authors: B
authors: Wu
authors: Yan
authors: Y
authors: Wei
authors: Feng-Qiang
authors: FQ
authors: Liu
authors: Qian
authors: Q
authors: Zhang
authors: Cun-Bang
authors: CB
authors: Wang
authors: Hai
authors: H
authors: Bai
mesh_terms: D001854:Bone Marrow Cells / Q000528:radiation effects*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000528:radiation effects*; D014965:X-Rays