title: The measurement of meat pigments by fibre-optic reflectance spectrophotometry using the Kubelka-Munk equation.
abstract: A reflectance spectrophotometry system with an adhesive pad type fibreoptic probe was developed to measure a wide range of absorbance. The performance of the instrument was evaluated by undertaking in vitro measurements of the reflectance spectra of metmyoglobin solution and meat pastes containing known amounts of oxy-, deoxy- and metmyoglobin. The absorbance of metmyoglobin solution in the range of 0-4 mg/dl as successfully distinguised, but at the upper range of 4 mg/ml the reflectance results were not in proportion to the concentration. All absorbances at 525 nm for meat pastes were also not in proportion to the concentration. These non-linearities were considered to be a result of back-scattering. Therefore, the K/S value of the Kubelka-Munk equation was applied in an effort the refine the fibre optic reflectance method. Positive correlations between the myoglobin concentration and the K/S values obtained from spectra of meat pastes were found.
authors: Sumitomo Electric Industries, Biomedical R&D Department 1-3, Shimaya 1-Chome, Konohana-Ku, Osaka, 554 Japan.
authors: M
authors: M
authors: Osawa