title: Clinical symptoms in fibromyalgia are better associated to lipid peroxidation levels in blood mononuclear cells rather than in plasma.
abstract: BACKGROUND
  We examined lipid peroxidation (LPO) in blood mononuclear cells (BMCs) and plasma, as a marker of oxidative damage, and its association to clinical symptoms in Fibromyalgia (FM) patients.
  
  
  METHODS
  We conducted a case-control and correlational study comparing 65 patients and 45 healthy controls. Clinical parameters were evaluated using the Fibromyalgia Impact Questionnaire (FIQ), visual analogues scales (VAS), and the Beck Depression Inventory (BDI). Oxidative stress was determined by measuring LPO in BMCs and plasma.
  
  
  RESULTS
  We found increased LPO levels in BMCs and plasma from FM patients as compared to normal control (P<0.001). A significant correlation between LPO in BMCs and clinical parameters was observed (r = 0.584, P<0.001 for VAS; r = 0.823, P<0.001 for FIQ total score; and r = 0.875, P<0.01 for depression in the BDI). We also found a positive correlation between LPO in plasma and clinical symptoms (r = 0.452, P<0.001 for VAS; r = 0.578, P<0.001 for FIQ total score; and r = 0.579, P<0.001 for depression in the BDI). Partial correlation analysis controlling for age and BMI, and sex, showed that both LPO in cells and plasma were independently associated to clinical symptoms. However, LPO in cells, but not LPO in plasma, was independently associated to clinical symptoms when controlling for depression (BDI scores).
  
  
  CONCLUSIONS
  The results of this study suggest a role for oxidative stress in the pathophysiology of fibromyalgia and that LPO in BMCs rather than LPO in plasma is better associated to clinical symptoms in FM.
authors: Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC and Centro de Investigación Biomédica en Red de Enfermedades Raras, ISCIII, Sevilla, Spain. mdcormor@upo.es
authors: Mario D
authors: MD
authors: Cordero
authors: Elísabet
authors: E
authors: Alcocer-Gómez
authors: Francisco J
authors: FJ
authors: Cano-García
authors: Manuel
authors: M
authors: De Miguel
authors: Angel M
authors: AM
authors: Carrión
authors: Plácido
authors: P
authors: Navas
authors: José A
authors: JA
authors: Sánchez Alcázar
mesh_terms: D000328:Adult; D001773:Blood Cells; D016022:Case-Control Studies; D005260:Female; D005356:Fibromyalgia / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010949:Plasma / Q000378:metabolism*