title: Divergent electrocardiographic responses to whole and particle-free diesel exhaust inhalation in spontaneously hypertensive rats.
abstract: Diesel exhaust (DE) is a major contributor to traffic-related fine particulate matter (PM)(2.5). Although inroads have been made in understanding the mechanisms of PM-related health effects, DE's complex mixture of PM, gases, and volatile organics makes it difficult to determine how the constituents contribute to DE's effects. We hypothesized that exposure to particle-filtered DE (fDE; gases alone) will elicit less cardiac effects than whole DE (wDE; particles plus gases). In addition, we hypothesized that spontaneously hypertensive (SH) rats will be more sensitive to the electrocardiographic effects of DE exposure than Wistar Kyoto rats (WKY; background strain with normal blood pressure). SH and WKY rats, implanted with telemeters to monitor electrocardiogram and heart rate (HR), were exposed once for 4 h to 150 μg/m(3) or 500 μg/m(3) of wDE (gases plus PM) or fDE (gases alone) DE, or filtered air. Exposure to fDE, but not wDE, caused immediate electrocardiographic alterations in cardiac repolarization (ST depression) and atrioventricular conduction block (PR prolongation) as well as bradycardia in SH rats. Exposure to wDE, but not fDE, caused postexposure ST depression and increased sensitivity to the pulmonary C fiber agonist capsaicin in SH rats. The only notable effect of DE exposure in WKY rats was a decrease in HR. Taken together, hypertension may predispose to the potential cardiac effects of DE and components of DE may have divergent effects with some eliciting immediate irritant effects (e.g., gases), whereas others (e.g., PM) trigger delayed effects potentially via separate mechanisms.
authors: Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina 27514, USA.
authors: Christina M
authors: CM
authors: Lamb
authors: Mehdi S
authors: MS
authors: Hazari
authors: Najwa
authors: N
authors: Haykal-Coates
authors: Alex P
authors: AP
authors: Carll
authors: Q Todd
authors: QT
authors: Krantz
authors: Charly
authors: C
authors: King
authors: Darrell W
authors: DW
authors: Winsett
authors: Wayne E
authors: WE
authors: Cascio
authors: Daniel L
authors: DL
authors: Costa
authors: Aimen K
authors: AK
authors: Farraj
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000139:chemically induced* / Q000175:diagnosis / Q000503:physiopathology; D002211:Capsaicin / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004562:Electrocardiography; D006339:Heart Rate / Q000187:drug effects*; D006973:Hypertension / Q000150:complications* / Q000503:physiopathology; D019570:Inhalation Exposure; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D018689:Sensory System Agents / Q000494:pharmacology; D013686:Telemetry; D013997:Time Factors; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D018689:Sensory System Agents; D001335:Vehicle Emissions; D002211:Capsaicin