title: Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.
abstract: We have developed a silicon photonic biosensing chip capable of multiplexed protein measurements in a biomolecularly complex cell culture matrix. Using this multiplexed platform combined with fast one-step sandwich immunoassays, we perform a variety of T cell cytokine secretion studies with excellent time-to-result. Using 32-element arrays of silicon photonic microring resonators, the cytokines interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), and tumor necrosis factor alpha (TNFα) were simultaneously quantified with high accuracy in serum-containing cell media. Utilizing this cytokine panel, secretion profiles were obtained for primary human Th0, Th1, and Th2 subsets differentiated from naïve CD4+ T cells, and we show the ability to discriminate between lineage commitments at early stages of culture differentiation. We also utilize this approach to probe the temporal secretion patterns of each T cell type using real-time binding analyses for direct cytokine quantitation down to ∼100 pM with just a 5 min-analysis.
authors: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
authors: Matthew S
authors: MS
authors: Luchansky
authors: Ryan C
authors: RC
authors: Bailey
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D008214:Lymphocytes / Q000378:metabolism; D017785:Photons; D012825:Silicon / Q000737:chemistry*
chemical_list: D016207:Cytokines; D012825:Silicon