title: Modulation of Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation by chronic iron overload in rat.
abstract: Iron constitutes a critical nutrient source for bacterial growth, so iron overload is a risk factor for bacterial infections. This study aimed at investigating the role of iron overload in modulating bacterial endotoxin-induced lung inflammation. Weaning male Wistar rats were intraperitoneally injected with saline or iron sucrose [15 mg kg(-1) body weight (bw), 3 times per week, 4 weeks]. They were then intratracheally injected with Pseudomonas aeruginosa lipopolysaccharide (LPS) (5 μg kg(-1) bw) or saline. Inflammatory indices were evaluated 4 or 18 h post-LPS/saline injection. At 4 h, LPS-treated groups revealed significant increases in the majority of inflammatory parameters (LPS-binding protein (LBP), immune cell recruitment, inflammatory cytokine synthesis, myeloperoxidase activity, and alteration of alveolar-capillary permeability), as compared with control groups. At 18 h, these parameters reduced strongly with the exception for LBP content and interleukin (IL)-10. In parallel, iron acted as a modulator of immune cell recruitment; LBP, tumor necrosis factor-α, cytokine-induced neutrophil chemoattractant 3, and IL-10 synthesis; and alveolar-capillary permeability. Therefore, P. aeruginosa LPS may only act as an acute lung inflammatory molecule, and iron overload may modulate lung inflammation by enhancing different inflammatory parameters. Thus, therapy for iron overload may be a novel and efficacious approach for the prevention and treatment of bacterial lung inflammations.
authors: Peritox Laboratory EA4285-UMI 01, Faculty of Medicine, Picardy Jules Verne University, Amiens, France.
authors: Bá Vuong
authors: BV
authors: Lê
authors: Hafida
authors: H
authors: Khorsi-Cauet
authors: Véronique
authors: V
authors: Bach
authors: Jérôme
authors: J
authors: Gay-Quéheillard
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D005290:Ferric Compounds / Q000008:administration & dosage; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid / Q000008:administration & dosage; D007501:Iron / Q000494:pharmacology*; D019190:Iron Overload / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008168:Lung / Q000276:immunology / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D014886:Weaning
chemical_list: D016207:Cytokines; D005290:Ferric Compounds; D008070:Lipopolysaccharides; D007501:Iron; D009195:Peroxidase; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid