title: Coagulation factor III (tissue  factor) is required for vascularization in zebrafish embryos.
abstract: Tissue  factor (coagulation factor III) is a cell surface receptor for coagulation  factor VII/VIIa; it was initially recognized as an initiator of the extrinsic  coagulation pathway. Recently, the zebrafish tissue factor gene (TF) has  been cloned. Paralogs encode coagulation factors IIIa and IIIb; both show  remarkable sequence identity to the human and mouse coagulation factor III  gene. It has been reported that TF could have additional properties that are  essential for normal embryonic development, since knockout of the murine  coagulation factor III gene resulted in 90% embryonic lethality. We examined  the role of coagulation factor IIIb (f3b) during zebrafish embryonic  development. Expression analysis revealed that endogenous f3b was  chronologically expressed in the pectoral fins and in the vicinity of the  pharynx. Knockout of f3b by injection of an f3b morpholino at the  one-to-two cell stage caused distinctive morphological defects in embryos,  including edema in the fourth brain ventricle at early embryonic stages and  occasional bleeding at later stages. Furthermore, f3b morphants  displayed abnormal vascular patterning. We conclude that f3b is required  for brain vascular development and for development of part of the somatic  vasculature during embryogenesis in the zebrafish.
authors: Key Laboratory of Molecular Medicine, Department of Biochemistry and Molecular Biology, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai, PR China.
authors: R F
authors: RF
authors: Zhou
authors: Y
authors: Y
authors: Liu
authors: Y X
authors: YX
authors: Wang
authors: W
authors: W
authors: Mo
authors: M
authors: M
authors: Yu
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000098:blood supply* / Q000378:metabolism; D005176:Factor XIII / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D009389:Neovascularization, Pathologic / Q000378:metabolism; D010641:Phenotype; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029961:Zebrafish Proteins; D005176:Factor XIII; D013925:Thromboplastin; C054380:factor XIIIb