title: Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions.
abstract: This method describes the combination of chemical cross-linking and high-resolution mass spectrometry for analyzing conformational changes in target proteins that are induced by drug binding. Our approach is exemplified for detecting conformational changes within the peroxisome proliferator-activated receptor alpha upon binding of low-molecular weight compounds, proving that our strategy provides a basis to efficiently characterize target protein-drug interactions.
authors: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.
authors: Mathias Q
authors: MQ
authors: Müller
authors: Andrea
authors: A
authors: Sinz
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D008024:Ligands; D008969:Molecular Sequence Data; D036103:Nanotechnology; D010080:Oxazoles / Q000378:metabolism; D047493:PPAR alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013223:Statistics as Topic; D013388:Succinimides / Q000737:chemistry; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D004952:Esters; C449302:GW 6471; D008024:Ligands; D010080:Oxazoles; D047493:PPAR alpha; D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins; D013388:Succinimides; D014443:Tyrosine; C001426:N-hydroxysuccinimide