title: [Study on the enzyme extraction process of hypericin by pectinase].
abstract: OBJECTIVE
  To explore the best enzyme and optimal conditions for extracting Hypericin from Hypericum perforatum.
  
  
  METHODS
  Chose the best enzyme from Pectinase, Xylanase, Glucanase, beta-Glucanase and Enzyme (SPE-007A). The effeet of solid-liquid ratio enzyme dosage, PH, temperature and the extraction time were investigated by L9 (3(4)) orthogonal design using extraction rate and the content of Hypericin as assessment index.
  
  
  RESULTS
  The best enzyme was Pectinase and the optimum extraction process was as follows: PH 4.6, enzyme dosage 1.5%, temperature 50 degrees C, extraction time 5 h, liquid-solid ratio 15 times.
  
  
  CONCLUSIONS
  This method is efficient and stable. It could be used in the future research of Hypericum perforatum.
authors: Henan University of TCM, Zhengzhou 450008, China. hpf_880129@163.com
authors: Peng-fei
authors: PF
authors: Hao
authors: Gao-ming
authors: GM
authors: Lei
authors: Fu-gang
authors: FG
authors: Liu
authors: Yun
authors: Y
authors: Yang
mesh_terms: D000704:Analysis of Variance; D000873:Anthracenes; D002851:Chromatography, High Pressure Liquid; D003910:Dextranase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020902:Hypericum / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011096:Polygalacturonase / Q000378:metabolism*; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000873:Anthracenes; D010569:Perylene; C004965:hypericin; D003910:Dextranase; D011096:Polygalacturonase