title: Effects of cadmium chloride and nonylphenol on the expression of StAR-related lipid transfer domain containing protein (START1) gene in aquatic midge, Chironomus riparius.
abstract: We identified and characterized a partial cDNA of StAR-related lipid transfer domain containing protein gene from Chironomus riparius (CrSTART1) having homology with human MLN64 and Drosophila melanogaster START1 (DmSTART1) and evaluated the effects of cadmium chloride (Cd) and nonylphenol (NP) on its expression. Pfam analysis identified the presence of two StAR-related lipid transfer (START) domains in CrSTART1 having several conserved amino acid residues, characteristic of the MLN64 and DmSTART1. The mRNA expression of CrSTART1 was observed in all developmental stages. The modulation in the mRNA expression of CrSTART1 was investigated after exposure to different concentrations Cd (0, 2, 10, and 20 mg/L) and NP (0, 10, 50, and 100 μg/L) for different time intervals in fourth instar larvae of C. riparius. Significant downregulation of CrSTART1 mRNA was observed after exposure to 2, 10 and 20 mg/L of Cd for 24, 48 and 72 h. Significant upregulation of CrSTART1 was observed after exposure to 10 and 50 μg/L of NP for 24, and 48 h period. At 100 μg/L of NP significant upregulation of CrSTART1 was observed after 12 h and downregulated after 24, 48 and 72 h.
authors: School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea.
authors: Prakash M Gopalakrishnan
authors: PM
authors: Nair
authors: Jinhee
authors: J
authors: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity*; D002352:Carrier Proteins / Q000235:genetics*; D002683:Chironomidae / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D010636:Phenols / Q000633:toxicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D019476:Insect Proteins; D010636:Phenols; D014874:Water Pollutants, Chemical; C025256:nonylphenol; D019256:Cadmium Chloride