title: Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides.
abstract: Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortium, which can hydrolyze methyl-parathion (MP) and other organophosphate pesticides. HPLC analysis showed that strain Cons002 is capable of degrading pesticides MP, parathion and phorate. Pulsed-field gel electrophoresis and 16S rRNA amplification were performed for strain characterization and identification, respectively, showing that the strain Cons002 is related to the genus Enterobacter sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of Enterobacter sp. Cons002. A gene called opdE (Organophosphate Degradation from Enterobacter) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene opdE had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene opdE, hydrolase activity was suppressed and Enterobacter sp. Cons002 had no growth with MP as a nutrients source.
authors: Laboratorio de Investigaciones Ambientales, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209, Cuernavaca, Morelos, Mexico.
authors: Concepción
authors: C
authors: Chino-Flores
authors: Edgar
authors: E
authors: Dantán-González
authors: Alejandra
authors: A
authors: Vázquez-Ramos
authors: Raunel
authors: R
authors: Tinoco-Valencia
authors: Rafael
authors: R
authors: Díaz-Méndez
authors: Enrique
authors: E
authors: Sánchez-Salinas
authors: Maria Luisa
authors: ML
authors: Castrejón-Godínez
authors: Fernando
authors: F
authors: Ramos-Quintana
authors: Maria Laura
authors: ML
authors: Ortiz-Hernández
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004754:Enterobacter / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D006867:Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D009943:Organophosphorus Compounds; D010575:Pesticides; D006867:Hydrolases