title: Repeated exposure to Aspergillus fumigatus conidia results in CD4+ T cell-dependent and -independent pulmonary arterial remodeling in a mixed Th1/Th2/Th17 microenvironment that requires interleukin-4 (IL-4) and IL-10.
abstract: Pulmonary arterial remodeling is a pathological process seen in a number of clinical disease states, driven by inflammatory cells and mediators in the remodeled artery microenvironment. In murine models, Th2 cell-mediated immune responses to inhaled antigens, such as purified Aspergillus allergen, have been reported to induce remodeling of pulmonary arteries. We have previously shown that repeated intranasal exposure of healthy C57BL/6 mice to viable, resting Aspergillus fumigatus conidia leads to the development of chronic pulmonary inflammation and the coevolution of Th1, Th2, and Th17 responses in the lungs. Our objective was to determine whether repeated intranasal exposure to Aspergillus conidia would induce pulmonary arterial remodeling in this mixed Th inflammatory microenvironment. Using weekly intranasal conidial challenges, mice developed robust pulmonary arterial remodeling after eight exposures (but not after two or four). The process was partially mediated by CD4+ T cells and by interleukin-4 (IL-4) production, did not require eosinophils, and was independent of gamma interferon (IFN-γ) and IL-17. Furthermore, remodeling could occur even in the presence of strong Th1 and Th17 responses. Rather than serving an anti-inflammatory function, IL-10 was required for the development of the Th2 response to A. fumigatus conidia. However, in contrast to previous studies of pulmonary arterial remodeling driven by the A. fumigatus allergen, viable conidia also stimulated pulmonary arterial remodeling in the absence of CD4+ T cells. Remodeling was completely abrogated in IL-10-/- mice, suggesting that a second, CD4+ T cell-independent, IL-10-dependent pathway was also driving pulmonary arterial remodeling in response to repeated conidial exposure.
authors: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
authors: Andrew B
authors: AB
authors: Shreiner
authors: Benjamin J
authors: BJ
authors: Murdock
authors: Amir A
authors: AA
authors: Sadighi Akha
authors: Nicole R
authors: NR
authors: Falkowski
authors: Paul J
authors: PJ
authors: Christensen
authors: Eric S
authors: ES
authors: White
authors: Cory M
authors: CM
authors: Hogaboam
authors: Gary B
authors: GB
authors: Huffnagle
mesh_terms: D000818:Animals; D001232:Aspergillus fumigatus / Q000276:immunology / Q000472:pathogenicity*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D019570:Inhalation Exposure; D016753:Interleukin-10 / Q000276:immunology*; D015847:Interleukin-4 / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000382:microbiology* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic; D013172:Spores, Fungal / Q000276:immunology / Q000472:pathogenicity; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10; D015847:Interleukin-4