title: Proteomic analysis of water soluble and myofibrillar protein changes occurring in dry-cured hams.
abstract: The myofibrillar fraction of raw ham muscles and dry-cured hams with different ripening times was extracted in denaturing and reducing conditions and subjected to two-dimensional gel electrophoresis. The two-dimensional maps gave overall pictures of the already noted progressive disappearance of actin, tropomyosin and myosin light chains during ripening. In addition, two fragments from Myosin Heavy Chain proteolysis, marked as myosin chain fragments MCF1 and MCF2, were identified by immunodetection and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Furthermore, a new form of actin on two-dimensional gel was identified by MALDI-TOF peptide mapping. In 12-month-old dry-cured ham, most myofibrillar proteins were completely hydrolyzed. At this stage of ripening, in fact, in some Parma and S. Daniele dry-cured ham samples, myosin heavy chain fragments and other unidentified neo-formed spots were found. Some of the sarcoplasmic proteins in water extracts from pork meat markedly decreased in amount or disappeared totally, during ripening. Surprisingly, two-dimensional gel electrophoresis maps of the water soluble protein fraction from dry-cured ham showed the presence of two spots identified as tropomyosin α- and β-chain. This result suggests that some of the saline soluble myofibrillar proteins can disappear from this fraction because of salt solubilization and not due to complete enzyme action. Two-dimensional gel electrophoresis (2-DGE) has proved a powerful tool to evaluate the enzymatic susceptibility of meat proteins and the evolution of protein map fragmentation throughout ripening process as well as a means of obtaining a standard fingerprinting map characterizing the final product.
authors: Dipartimento di Produzione Animale, Università di Bari, via G. Amendola 165/A, 70126 Bari, Italy.
authors: Aldo Di
authors: AD
authors: Luccia
authors: Gianluca
authors: G
authors: Picariello
authors: Giuseppina
authors: G
authors: Cacace
authors: Andrea
authors: A
authors: Scaloni
authors: Michele
authors: M
authors: Faccia
authors: Vitantonio
authors: V
authors: Liuzzi
authors: Giovanna
authors: G
authors: Alviti
authors: Salvatore Spagna
authors: SS
authors: Musso