title: Incidence in diverse pig populations of an IGF2 mutation with potential influence on meat quality and quantity: An assay based on real time PCR (RT-PCR).
abstract: IGF2, insulin-like growth factor 2, is implicated in myogenesis and lean meat content. A mutation in a single base (A for G substitution) of the gene for IGF2 (position 3072 in intron 3) has been recently described as the cause of a major QTL effect on muscle growth in pigs [Van Laere, A. S, Nguyen, M., Braunschweig, M., Nezer, C., Collete, C., & Moreau, L. et al. (2003). Nature, 425, 832-836]. We describe here a rapid assay based on real time PCR (RT-PCR) to detect this mutation. We have evaluated the incidence of the mutation in commercial pig crosses, in three populations of purebred Iberian or Iberian×Duroc crosses, and in cured meat products and wild boars. The incidence of the mutation varies among these groups. Penetrance of the A mutation is about 80% in the commercial population. Purebred Iberian pigs were all homozygous G/G whereas crosses of Iberian pigs were heterozygous (90%) or homozygous A/A (10%). The implications of this gene for the selection of Iberian pigs are discussed.
authors: Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
authors: José Alberto
authors: JA
authors: Carrodeguas
authors: Carmen
authors: C
authors: Burgos
authors: Carlos
authors: C
authors: Moreno
authors: Ana Cristina
authors: AC
authors: Sánchez
authors: Sonia
authors: S
authors: Ventanas
authors: Luis
authors: L
authors: Tarrafeta
authors: José Antonio
authors: JA
authors: Barcelona
authors: Maria Otilia
authors: MO
authors: López
authors: Rosa
authors: R
authors: Oria
authors: Pascual
authors: P
authors: López-Buesa