title: Tight and uniform layer of covalently bound aminoethylophenyl groups perpendicular to gold surface for attachment of biomolecules.
abstract: Strongly adhered layers of the compound with the primary amino group directed toward the solution were obtained at the gold surface by chronoamperometric electroreduction of 4-aminoethylobenzenodiazonium salt (AEBD) in acetonitrile solution at appropriately selected potential. The used techniques (EQCM, AFM, EIS, PM, IRRAS) showed that the nature and thickness of formed aminoethylophenyl layer strongly depend on the potential applied to the electrode. Electroreduction of AEBD salt at a potential more negative than -0.6 V (vs Ag/AgCl) leads to about monolayer on the gold surface. Additionally, such a layer was very tight and uniform. The electrochemical measurements indicate that the efficient and precise attachment of biomolecules to the aminoethylophenyl layer is only possible when this layer is formed at appropriate potential. This was shown for ss- and dsDNA.
authors: Department of Chemistry, University of Warsaw, Warsaw.
authors: Michal
authors: M
authors: Fau
authors: Agata
authors: A
authors: Kowalczyk
authors: Piotr
authors: P
authors: Olejnik
authors: Anna M
authors: AM
authors: Nowicka
mesh_terms: D000097:Acetonitriles; D004247:DNA / Q000737:chemistry*; D003979:Diazonium Compounds / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
chemical_list: D000097:Acetonitriles; D003979:Diazonium Compounds; D006046:Gold; D004247:DNA; C032159:acetonitrile