title: Heterologous production of bisucaberin using a biosynthetic gene cluster cloned from a deep sea metagenome.
abstract: A siderophore biosynthetic gene cluster was cloned from a metagenomic library generated from deep sea sediment. The gene cluster was successfully expressed in Escherichia coli to produce bisucaberin, a siderophore originally reported from the marine bacterium Alteromonas haloplanktis. The cloned bisucaberin biosynthetic gene cluster was moderately similar to that of the known bisucaberin producer Vibrio salmonicida. However, the cloned gene cluster consists of four genes rather than three genes found in the V. salmonicida cluster. The low overall homology of the amino acid and nucleotide sequences with those of other species suggests that the cloned genes were derived from one of the unsequenced bacteria including uncultured species.
authors: Priority Organization for Innovation and Excellence, Kumamoto University, Kumamoto, Japan. fujitam@kumamoto-u.ac.jp
authors: Masaki J
authors: MJ
authors: Fujita
authors: Nobutada
authors: N
authors: Kimura
authors: Hisayoshi
authors: H
authors: Yokose
authors: Masami
authors: M
authors: Otsuka
mesh_terms: D000595:Amino Acid Sequence; D059001:Aquatic Organisms / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D054892:Metagenome; D005810:Multigene Family; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017262:Siderophores / Q000096:biosynthesis / Q000235:genetics; D014733:Vibrio / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010456:Peptides, Cyclic; D017262:Siderophores; C054842:bisucaberin