title: Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma.
abstract: BACKGROUND
  Although synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to comparative genomic hybridization (CGH) data published in the literature, the simple and complex karyotypes show a correlation between the prognosis and clinical outcome. In addition, the connection between DNA ploidy and clinical course is controversial. The aim of this study was using a fine-tuning interpretation of our DNA ploidy results and to compare these with metaphase high-resolution CGH (HR-CGH) results.
  
  
  METHODS
  DNA ploidy was determined on Feulgen-stained smears in 56 synovial sarcoma cases by image cytometry; follow up was available in 46 cases (average: 78 months). In 9 cases HR-CGH analysis was also available.
  
  
  RESULTS
  10 cases were found DNA-aneuploid, 46 were DNA-diploid by image cytometry. With fine-tuning of the diploid cases according to the 5c exceeding events (single cell aneuploidy), 33 cases were so called "simple-diploid" (without 5c exceeding events) and 13 cases were "complex-diploid"; containing 5c exceeding events (any number). Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found.
  
  
  CONCLUSIONS
  Our results show a correlation between the DNA-ploidy, a fine-tuned DNA-ploidy and the HR-CGH results. Furthermore, we found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05).
authors: 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary.
authors: Zsófia
authors: Z
authors: Balogh
authors: Zsuzsanna
authors: Z
authors: Szemlaky
authors: Miklós
authors: M
authors: Szendroi
authors: Imre
authors: I
authors: Antal
authors: Zsuzsanna
authors: Z
authors: Pápai
authors: László
authors: L
authors: Fónyad
authors: Gergo
authors: G
authors: Papp
authors: Yi C
authors: YC
authors: Changchien
authors: Zoltán
authors: Z
authors: Sápi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D055028:Comparative Genomic Hybridization; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D019044:Image Cytometry; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D013584:Sarcoma, Synovial / Q000235:genetics* / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology*; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm