title: Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring.
abstract: Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.
authors: IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, Milan, Italy.
authors: A B
authors: AB
authors: Lovati
authors: E
authors: E
authors: Vianello
authors: G
authors: G
authors: Talò
authors: C
authors: C
authors: Recordati
authors: L
authors: L
authors: Bonizzi
authors: E
authors: E
authors: Galliera
authors: M
authors: M
authors: Broggini
authors: M
authors: M
authors: Moretti
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000166:cytology / Q000637:transplantation; D019902:Chondrocytes / Q000166:cytology* / Q000637:transplantation*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous
chemical_list: D058185:Magnetite Nanoparticles