title: [Pathological changes of grafted vein after repairing lacrimal canaliculus with autogenous vein graft in rabbits].
abstract: OBJECTIVE
  To observe the pathological changes of grafted vein after repairing lacrimal canaliculus with autogenous vein graft in rabbits.
  
  
  METHODS
  Experimental research. Sixty rabbits were used in the present study. One side of lacrimal canaliculus was disconnected to produce an experimental model. The canaliculus injury was repaired by autogenous vein graft. Pathological changes and cell proliferation of grafted vein segment were observed by routine HE stain and immunohistochemistry. The cell ultrastructure changes were observed by scanning electron microscope at 1, 3, 5, 7, 14 and 28 day after the operation.
  
  
  RESULTS
  Experimental models were successfully established in 60 rabbits. Endothelial cells of grafted vein segment gradually disappeared after operation, the vein lumen was covered by canalicular epithelial cells 7 days after the operation. At the same time some variations of epithelial cells and exposed collagen fiber structure in subendothelial layer were found by scanning electron microscope. The vein lumen was completely covered with stratified squamous epithelium 28 days after the operation and no epithelial defect could be found by scanning electron microscope. Inflammatory cell infiltration, smooth muscle cell degeneration and fibroblast proliferation were observed in the subepithelial connective tissues. The reconstruction of propria lamina required more than 28 day.
  
  
  CONCLUSIONS
  After autogenous vein graft repairing lacrimal canaliculus, endothelial cells of grafted vein were replaced by canalicular epithelial cells and the propria lamina of grafted vein segment was reconstructed.
authors: Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors: Zhi-gang
authors: ZG
authors: Li
authors: Yu
authors: Y
authors: Zhu
authors: Xiao-peng
authors: XP
authors: Zhang
authors: Fang
authors: F
authors: Liu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004195:Disease Models, Animal; D007765:Lacrimal Apparatus / Q000601:surgery; D011184:Postoperative Period; D011817:Rabbits; D014182:Transplantation, Autologous; D014680:Veins / Q000473:pathology* / Q000637:transplantation*