title: Fluorescent reporters of monoamine transporter distribution and function.
abstract: Serotonin is a monoamine serving as a chemical messenger in diverse brain regions, as well as in blood and various other organs. We synthesized six ethylamine functionalized fluorophores as fluorescent probes for serotonin. The one with best spectral properties and aqueous solubility, 6-amino-2-(2-aminoethyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione, was studied in detail both in vivo and in vitro. It was shown to act as a ligand for serotonin transporter (SERT) without acute cerebral or cardiovascular toxicity or adverse effects. Fluorescent serotonin analogs can be used for direct visualization of SERT distribution and activity in live tissue.
authors: Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33124-0431, USA.
authors: Sharanappa M
authors: SM
authors: Bagale
authors: Adrienne S
authors: AS
authors: Brown
authors: Melissa M Carballosa
authors: MM
authors: Gonzalez
authors: Alberto
authors: A
authors: Vitores
authors: Teresa L
authors: TL
authors: Micotto
authors: N S Saleesh
authors: NS
authors: Kumar
authors: Ian D
authors: ID
authors: Hentall
authors: James N
authors: JN
authors: Wilson
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D007546:Isoquinolines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D050486:Serotonin Plasma Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D017367:Selective Serotonin Reuptake Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; D050486:Serotonin Plasma Membrane Transport Proteins; D017367:Selective Serotonin Reuptake Inhibitors; C039109:isoquinoline