title: Kampo medicine "Dai-kenchu-to" prevents CPT-11-induced small-intestinal injury in rats.
abstract: OBJECTIVE
  The key anticancer agent, CPT-11 (irinotecan hydrochloride), induces severe diarrhea clinically. We investigated the effect of a Kampo medicine, Dai-kenchu-to (DKT), on CPT-11-induced intestinal injuries in rats.
  
  
  METHODS
  Twenty-four male Wistar rats were divided into three groups: a control group; a CPT-11 group, given CPT-11 150 mg/kg intraperitoneally for 2 days; and a DKT group, given DKT 300 mg/kg orally for 5 days with CPT-11 150 mg/kg intraperitoneally on days 4 and 5. The rats were killed on day 6.
  
  
  RESULTS
  Interleukin (IL)-1β, IL-12, interferon (IFN)-γ, and tumor necrosis factor-α expression in the small intestine of the CPT-11 group was significantly higher than that of the control group. Interleukin-1β and IFN-γ expression was improved significantly by DKT (P < 0.05). The number and height of jejuna villi, injury score, and apoptosis index in the CPT-11 group were improved significantly by DKT (P < 0.05).
  
  
  CONCLUSIONS
  DKT suppressed CPT-11 induced inflammatory cytokines and apoptosis in the intestinal mucosa and maintained the mucosal integrity.
authors: Department of Surgery, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
authors: Motoya
authors: M
authors: Chikakiyo
authors: Mitsuo
authors: M
authors: Shimada
authors: Toshihiro
authors: T
authors: Nakao
authors: Jun
authors: J
authors: Higashijima
authors: Kozo
authors: K
authors: Yoshikawa
authors: Masanori
authors: M
authors: Nishioka
authors: Takashi
authors: T
authors: Iwata
authors: Nobuhiro
authors: N
authors: Kurita
mesh_terms: D000818:Animals; D017209:Apoptosis; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000633:toxicity; D003967:Diarrhea / Q000139:chemically induced / Q000517:prevention & control*; D020287:In Situ Nick-End Labeling; D007371:Interferon-gamma / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D000077146:Irinotecan; D008297:Male; D020835:Medicine, Kampo; D005894:Panax; D010936:Plant Extracts; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D032106:Zanthoxylum; D030521:Zingiberaceae
chemical_list: D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C121414:dai-kenchu-to; D018664:Interleukin-12; D000077146:Irinotecan; D007371:Interferon-gamma; D002166:Camptothecin