title: Localization and activity of the calcineurin catalytic and regulatory subunit complex at the septum is essential for hyphal elongation and proper septation in Aspergillus fumigatus.
abstract: Calcineurin, a heterodimer composed of the catalytic (CnaA) and regulatory (CnaB) subunits, plays key roles in growth, virulence and stress responses of fungi. To investigate the contribution of CnaA and CnaB to hyphal growth and septation, ΔcnaB and ΔcnaAΔcnaB strains of Aspergillus fumigatus were constructed. CnaA colocalizes to the contractile actin ring early during septation and remains at the centre of the mature septum. While CnaB's septal localization is CnaA-dependent, CnaA's septal localization is CnaB-independent, but CnaB is required for CnaA's function at the septum. Catalytic null mutations in CnaA caused stunted growth despite septal localization of the calcineurin complex, indicating the requirement of calcineurin activity at the septum. Compared to the ΔcnaA and ΔcnaB strains, the ΔcnaAΔcnaB strain displayed more defective growth and aberrant septation. While three Ca(2+) -binding motifs in CnaB were sufficient for its association with CnaA at the septum, the amino-terminal arginine-rich domains (16-RRRR-19 and 44-RLRKR-48) are dispensable for septal localization, yet required for complete functionality. Mutation of the 51-KLDK-54 motif in CnaB causes its mislocalization from the septum to the nucleus, suggesting it is a nuclear export signal sequence. These findings confirm a cooperative role for the calcineurin complex in regulating hyphal growth and septation.
authors: Department of Pediatrics, Division of Pediatric Infectious Diseases, Duke University Medical Center, Durham, NC, USA.
authors: Praveen Rao
authors: PR
authors: Juvvadi
authors: Jarrod R
authors: JR
authors: Fortwendel
authors: Luise E
authors: LE
authors: Rogg
authors: Kimberlie A
authors: KA
authors: Burns
authors: Scott H
authors: SH
authors: Randell
authors: William J
authors: WJ
authors: Steinbach
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D005656:Fungal Proteins / Q000378:metabolism; D017353:Gene Deletion; D025301:Hyphae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D005656:Fungal Proteins; D021122:Protein Subunits; D019703:Calcineurin; D002118:Calcium