title: Bcl-2/IgH expression in minimal bone marrow infiltration by follicular             lymphoma cells.
abstract: The purpose of this study was to investigate the roles of bcl-2 chromosomal             translocation and Bcl-2 protein expression in follicular lymphoma (FL) minimal             bone marrow (BM) infiltration. We identified the same bcl-2/IgH fusion gene in             paraffin-embedded lymph node (LN) samples and BM samples using immunohistochemistry             (IHC), immunocytochemistry (ICC), cytologic morphology and fluorescence in situ             hybridization (FISH). The presence of the Bcl-2/IgH fusion gene in the BM samples             and paraffin-embedded LN samples from 56 patients with follicular lymphomas was             detected using FISH. The Bcl-2 protein levels in BM and paraffin-embedded tissues             were quantified using ICC and IHC, respectively. Approximately 78.6% (44/56) of             the paraffin‑embedded LN tissue sections that underwent FISH analysis had a bcl-2/IgH             translocation. The primary lesion was also positive for the bcl-2/IgH fusion gene,             as were the BM minimal infiltrates. The bcl-2/IgH rearrangement occurred in 88.6%             (39/44) of the BM specimens. The bcl-2/IgH recombination rate in stage III/IV             cancers was significantly different to that observed in stage I/II cancers (p=0.041).             In 59% (23/39) of the cases with t(14;18), Bcl-2 was found to be present as assessed             by ICC. Positive Bcl-2 ICC staining and the t(14;18) translocation (as detected             using FISH) were positively correlated (p=0.028). We then applied the FISH method             to slides that had previously been morphologically evaluated using Wright-Giemsa             staining; any slides with at least one abnormal cell were subjected to FISH analysis             following staining. The assessment of bcl-2/IgH translocation status may contribute             to the better detection of minimal BM infiltration by FL cells. Utilizing FISH             and cytologic morphology techniques allows for earlier and more accessible BM             examination.
authors: Clinical Laboratory Department, Chao-Yang Hospital, Capital Medical University, Beijing 100020, PR China.
authors: Yi-Qun
authors: YQ
authors: Che
authors: Peng
authors: P
authors: Liu
authors: Yue
authors: Y
authors: Wang
authors: Chang-Gong
authors: CG
authors: Zhang
authors: Ya-Ling
authors: YL
authors: Han
authors: Di
authors: D
authors: Shen
authors: Ying
authors: Y
authors: Zhang
authors: Cui-Ling
authors: CL
authors: Zheng
authors: Jun
authors: J
authors: Qi
authors: Qing-Tao
authors: QT
authors: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D015331:Cohort Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008224:Lymphoma, Follicular / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains; D019253:Proto-Oncogene Proteins c-bcl-2