title: Identification of transcriptome profiles and signaling pathways for the allelochemical juglone in rice roots.
abstract: Juglone (5-hydroxy-1,4-naphthoquinone) is known allelochemical, but its molecular mode of action is not well understood. We found that juglone induced reactive oxygen species production and calcium accumulation. To gain more insight into these cellular responses, we performed large-scale analysis of the rice transcriptome during juglone stress. Exposure to juglone triggered changes in transcript levels of genes related to cell growth, cell wall formation, chemical detoxification, abiotic stress response and epigenesis. The most predominant transcription-factor families were AP2/ERF, HSF, NAC, C2H2, WRKY, MYB and GRAS. Gene expression profiling of juglone-treated rice roots revealed upregulated signaling and biosynthesis of abscisic acid and jasmonic acid and inactivation of gibberellic acid. In addition, juglone upregulated the expression of two calcium-dependent protein kinases (CDPKs), 6 mitogen-activated protein kinase (MAPK) genes and 1 MAPK gene and markedly increased the activities of a CDPK-like kinase and MAPKs. Further characterization of these juglone-responsive genes may be helpful for better understanding the mechanisms of allelochemical tolerance in plants.
authors: Department of Life Sciences, National Cheng Kung University, Tainan City 701, Taiwan.
authors: Wen-Chang
authors: WC
authors: Chi
authors: Shih-Feng
authors: SF
authors: Fu
authors: Tsai-Lien
authors: TL
authors: Huang
authors: Yun-An
authors: YA
authors: Chen
authors: Chi-Cien
authors: CC
authors: Chen
authors: Hao-Jen
authors: HJ
authors: Huang
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D009285:Naphthoquinones / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D020928:Mitogen-Activated Protein Kinases; D009285:Naphthoquinones; D010940:Plant Proteins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C005134:juglone