title: Characterization of RNase MRP RNA and novel snoRNAs from Giardia intestinalis and Trichomonas vaginalis.
abstract: BACKGROUND
  Eukaryotic cells possess a complex network of RNA machineries which function in RNA-processing and cellular regulation which includes transcription, translation, silencing, editing and epigenetic control. Studies of model organisms have shown that many ncRNAs of the RNA-infrastructure are highly conserved, but little is known from non-model protists. In this study we have conducted a genome-scale survey of medium-length ncRNAs from the protozoan parasites Giardia intestinalis and Trichomonas vaginalis.
  
  
  RESULTS
  We have identified the previously 'missing' Giardia RNase MRP RNA, which is a key ribozyme involved in pre-rRNA processing. We have also uncovered 18 new H/ACA box snoRNAs, expanding our knowledge of the H/ACA family of snoRNAs.
  
  
  CONCLUSIONS
  Results indicate that Giardia intestinalis and Trichomonas vaginalis, like their distant multicellular relatives, contain a rich infrastructure of RNA-based processing. From here we can investigate the evolution of RNA processing networks in eukaryotes.
authors: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors: Xiaowei S
authors: XS
authors: Chen
authors: David
authors: D
authors: Penny
authors: Lesley J
authors: LJ
authors: Collins
mesh_terms: D001483:Base Sequence; D020451:Contig Mapping; D004722:Endoribonucleases / Q000235:genetics*; D016829:Giardia lamblia / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015800:Protozoan Proteins / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000235:genetics; D020537:RNA, Small Nucleolar / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014246:Trichomonas vaginalis / Q000235:genetics*
chemical_list: D015800:Protozoan Proteins; D016053:RNA, Protozoan; D020537:RNA, Small Nucleolar; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease