title: [Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD].
abstract: OBJECTIVE
  To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.
  
  
  METHODS
  An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.
  
  
  RESULTS
  The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.
  
  
  CONCLUSIONS
  Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.
authors: Key Laboratory of Traditional Chinese Medicine Preparation of Guangdong Province, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. wangxy1225@126.com
authors: Xinyu
authors: X
authors: Wang
authors: Xiaomei
authors: X
authors: Tan
authors: Mingze
authors: M
authors: Gao
authors: Jinlong
authors: J
authors: Peng
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000032:analysis*; D011392:Proline / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D014426:Turtles
chemical_list: D011392:Proline; D005998:Glycine