title: A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle.
abstract: We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluorescent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real-time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially modulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valuable bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts.
authors: Food Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors: Eriko
authors: E
authors: Chiba
authors: Julio
authors: J
authors: Villena
authors: Shoichi
authors: S
authors: Hosoya
authors: Naoya
authors: N
authors: Takanashi
authors: Tomoyuki
authors: T
authors: Shimazu
authors: Hisashi
authors: H
authors: Aso
authors: Masanori
authors: M
authors: Tohno
authors: Yoshihito
authors: Y
authors: Suda
authors: Yasushi
authors: Y
authors: Kawai
authors: Tadao
authors: T
authors: Saito
authors: Kenji
authors: K
authors: Miyazawa
authors: Fang
authors: F
authors: He
authors: Haruki
authors: H
authors: Kitazawa
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases; D002454:Cell Differentiation; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology*; D007413:Intestinal Mucosa / Q000166:cytology*; D007778:Lactobacillus / Q000502:physiology*; D019936:Probiotics; D048249:Streptococcus thermophilus / Q000502:physiology*; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism
chemical_list: D051196:Toll-Like Receptor 3