title: Construction of a potentiometric glutamate biosensor for determination of glutamate in some real samples.
abstract: The potentiometric glutamate biosensor based on ammonium-selective poly(vinylchloride) (PVC) membrane electrode was constructed by chemically immobilizing glutamate oxidase. Ammonium ions produced after an enzymatic reaction were determined potentiometrically. We determined the optimum working conditions of the biosensor such as buffer concentration, buffer pH, lifetime, response time, linear working range, kinetic constants (K(m) and V(max)) of glutamate oxidase enzyme used for biosensor construction values, and other response characteristics. Additionally, glutamate assay in some real samples such as chicken bullion, healthy human serum, and commercial multipower amino acid mixture were also successfully carried out. The results showed good agreement with previously reported values.
authors: Yildiz Technical University, Faculty of Science and Arts, Department of Chemistry, Esenler, Stanbul, Turkey.
authors: Demet
authors: D
authors: Y Lmaz
authors: Emine
authors: E
authors: Karaku
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002021:Buffers; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005503:Food Additives / Q000737:chemistry; D018698:Glutamic Acid / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008567:Membranes, Artificial; D011143:Polyvinyl Chloride / Q000737:chemistry; D011199:Potentiometry / Q000379:methods*; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000596:Amino Acids; D002021:Buffers; D004800:Enzymes, Immobilized; D005503:Food Additives; D008567:Membranes, Artificial; D000644:Quaternary Ammonium Compounds; D018698:Glutamic Acid; D011143:Polyvinyl Chloride; D000594:Amino Acid Oxidoreductases; C020651:D-glutamate oxidase