title: Expression and functional analysis of NUCLEAR FACTOR-Y, subunit B genes in barley.
abstract: NUCLEAR FACTOR-Y, subunit B (NF-YB) comprises a multigene family in plants and has been shown to play important roles in growth, development, and response to environmental stress. In this study, five NF-YBs containing the full-length coding region were obtained from barley (Hordeum vulgare) through database sequence analysis, cloning, and sequencing. Sequence alignment and phylogenetic analysis showed that HvNF-YB3 and HvNF-YB1 were clustered with NF-YB2 and NF-YB3 in Arabidopsis, suggesting these NF-YBs are evolutionary and functionally related. To test this hypothesis, HvNF-YB3 and HvNF-YB1 were overexpressed in Arabidopsis. Overexpression of HvNF-YB1 greatly promoted early flowering in Arabidopsis, supporting that HvNF-YB1may have conserved gene function in flowering time control as NF-YB2 and NF-YB3 in Arabidopsis. Overexpression of HvNF-YB3 in Arabidopsis had no effect on flowering time. An analysis of barley single-nucleotide polymorphism (SNP) data, however, revealed a significant association between an HvNF-YB3 SNP and heading date. While it is unknown whether HvNF-YB3 directly contributes to heading date regulation, the results implied that HvNF-YB3 may also have conserved function in flowering time (heading date in barley) control. Further studies are needed to directly verify these gene functions in barley. Barley NF-YBs showed different expression patterns associated with tissue types, developmental stages, and response to different stress treatments, suggesting that barley NF-YBs may be involved in other physiological processes.
authors: Department of Plants, Soils, and Climate, Utah State University, Logan, UT 84322, USA. liangmx@njau.edu.cn
authors: Mingxiang
authors: M
authors: Liang
authors: David
authors: D
authors: Hole
authors: Jixiang
authors: J
authors: Wu
authors: Tom
authors: T
authors: Blake
authors: Yajun
authors: Y
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D023081:CCAAT-Binding Factor / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010641:Phenotype; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D021122:Protein Subunits; D016415:Sequence Alignment; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D023081:CCAAT-Binding Factor; D021122:Protein Subunits; D014157:Transcription Factors; C402244:nuclear factor Y