title: Demineralised human dentine matrix stimulates the expression of VEGF and accelerates the bone repair in tooth sockets of rats.
abstract: OBJECTIVE
  In this study we investigated the possible use of human demineralised dentine matrix (DHDM), obtained from the extracted teeth, as bone graft material and evaluated the expression of vascular endothelial growth factor (VEGF) induced by this material in the healing process of tooth sockets of rats.
  
  
  METHODS
  To evaluate bone regeneration and expression of VEGF induced by DHDM, thirty-two male Wistar rats weighing approximately 200 g were used. After maxillary second molar extraction, the left sockets were filled with DHDM and the right sockets were naturally filled by blood clot (control). The animals were sacrificed at 3, 7, 14 and 21 days after surgery and upper maxillaries were processed for histological, morphometric and immunohistochemical analyses. DHDM was used to evaluate the mechanical effect of bone graft material into sockets. Expression of VEGF was determined by immunohistochemistry in all groups.
  
  
  RESULTS
  Our results demonstrated a significant increase in the newly formed bone tissue in sockets of 7, 14 and 21 days and a significant increase in VEGF expression at days 7 and 14 on treated sockets.
  
  
  CONCLUSIONS
  Our results showed that DHDM increases the expression of VEGF and accelerates the healing process in rats tooth sockets, by stimulating bone deposition and also vessels formation. These results suggest that DHDM has osteoinductive/osteoconductive potential and may represent an efficient grafting material on guided bone regeneration.
authors: Laboratory of Biopathology and Molecular Biology, Av. Nenê Sabino, 1801, University of Uberaba, Uberaba, Minas Gerais 38055-500, Brazil.
authors: Cláudio R
authors: CR
authors: Reis-Filho
authors: Elisângela R
authors: ER
authors: Silva
authors: Adalberto B
authors: AB
authors: Martins
authors: Fernanda F
authors: FF
authors: Pessoa
authors: Paula V N
authors: PV
authors: Gomes
authors: Mariana S C
authors: MS
authors: de Araújo
authors: Melissa N
authors: MN
authors: Miziara
authors: José B
authors: JB
authors: Alves
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D003804:Dentin; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008437:Maxilla / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014081:Tooth Extraction; D020390:Tooth Socket / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D015415:Biomarkers; C093073:Demineralized Dentin Matrix; D042461:Vascular Endothelial Growth Factor A