title: Genome size determination using flow cytometry of propidium iodide-stained nuclei.
abstract: With the rapid expansion of whole-genome sequencing and other genomic studies in nonmodel -organisms, there is a growing demand for robust and user-friendly methods for estimating eukaryotic genome sizes across a broad range of taxa. Propidium iodide (PI) staining with flow cytometry is a powerful method for genome sizing because it is relatively fast, works with a wide variety of materials, and provides information on a very large number of nuclei. In this method, nuclei are stained with PI, which intercalates into the major groove of DNA. Unknown samples are typically costained with standard nuclei of a known genome size, and the relative fluorescence is used to calculate the genome size of the unknown.
authors: Locus of Development, Inc., San Francisco, CA, USA.
authors: Emily E
authors: EE
authors: Hare
authors: J Spencer
authors: JS
authors: Johnston
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D002645:Chickens / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005629:Frozen Sections; D059646:Genome Size / Q000235:genetics*; D006793:Houseflies / Q000235:genetics; D011419:Propidium / Q000378:metabolism*; D013194:Staining and Labeling; D013223:Statistics as Topic
chemical_list: D011419:Propidium