title: VEGF-A immunohistochemical and mRNA expression in tissues and its serum levels in potentially malignant oral lesions and oral squamous cell carcinomas.
abstract: The aim of the study was to investigate whether the estimation of circulating Vascular endothelial growth factor-A (VEGF-A) levels by ELISA could be used as surrogate of VEGF-A expression in tissues of pre-malignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC) as compared to that in healthy controls. The study samples comprised of tissue and blood samples from 60 PMOLs, 60 OSCC, and 20 healthy controls. Serum VEGF-A levels were determined by an ELISA based assay (Quantikine human VEGF; R & D System, Minneapolis USA). Tissue VEGF-A expression and microvessel density (MVD) were assessed by immunohistochemistry (IHC) using antibodies against VEGF-A and CD-34 on formalin fixed paraffin embedded (FFPE) tissue sections. VEGF-A mRNA expression was analyzed by real-time PCR in snap frozen tissues. Serum VEGF-A levels and immunohistochemical VEGF-A expression were significantly high in PMOLs and OSCC in comparison with controls. VEGF mRNA gene expression showed more than 50-fold increase in PMOLs and OSCC. VEGF-A levels in serum correlated in a linear fashion with the tissue expression in oral pre-malignant and malignant lesions, suggesting that the serum levels may serve as surrogate material for tissue expression of VEGF-A.
authors: Department of Pathology, Chhatrapati Shahuji Maharaj Medical University, Lucknow 226003, Uttar Pradesh, India.
authors: Seema
authors: S
authors: Nayak
authors: Madhu Mati
authors: MM
authors: Goel
authors: Saumya
authors: S
authors: Chandra
authors: Vikram
authors: V
authors: Bhatia
authors: Divya
authors: D
authors: Mehrotra
authors: Sandeep
authors: S
authors: Kumar
authors: Annu
authors: A
authors: Makker
authors: S K
authors: SK
authors: Rath
authors: S P
authors: SP
authors: Agarwal
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D016022:Case-Control Studies; D055088:Early Detection of Cancer / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009062:Mouth Neoplasms / Q000097:blood* / Q000473:pathology; D011230:Precancerous Conditions / Q000097:blood* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A