title: Homing of placenta-derived mesenchymal stem cells after perinatal intracerebral transplantation in a rat model.
abstract: OBJECTIVE
  The aim of this study is to assess early homing of placenta-derived stem cells after perinatal intracerebral transplantation in rats.
  
  
  METHODS
  Neonatal Wistar rats (2-4 days old) were anesthetized, and 250,000 human placenta-derived mesenchymal stem cells (MSC) injected into the lateral ventricle or the paraventricular white matter using a stereotactic frame. Donor MSC were detected by immunohistochemistry using an antihuman HLA-ABC antibody.
  
  
  RESULTS
  In all, 84% of the animals survived the transplantation. Donor cells were detected in the brain ventricle 1-2 hours posttransplantation. After 4 hours, donor cells migrated throughout the ventricular system. At 1-4 weeks after transplantation, some cells had migrated into the periventricular white matter.
  
  
  CONCLUSIONS
  Human placenta-derived MSC were successfully transplanted into the lateral ventricles of neonatal rats. Donor cells survived, homed, and migrated in the recipient brains. Proliferation and differentiation analysis and functional tests will assess the therapeutic effects of stem cell transplantation.
authors: Department of Obstetrics and Gynecology, University Hospital Berne, Berne, Switzerland.
authors: Andreina
authors: A
authors: Schoeberlein
authors: Martin
authors: M
authors: Mueller
authors: Ursula
authors: U
authors: Reinhart
authors: Ruth
authors: R
authors: Sager
authors: Marianne
authors: M
authors: Messerli
authors: Daniel V
authors: DV
authors: Surbek
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D006801:Humans; D020547:Lateral Ventricles / Q000166:cytology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009413:Nerve Fibers, Myelinated; D051381:Rats; D017208:Rats, Wistar