title: [Isolation of alkaloids from Corydalis saxicola by high-speed counter-current chromatography].
abstract: OBJECTIVE
  To develop a method for the rapid isolation of alkaloids from the crude extract of Corydalis saxicola.
  
  
  METHODS
  High-speed counter-current chromatography (HSCCC) was applied for the separation of alkaloids from Corydalis saxicola, with the biphase solvent systems comprised n-butanol-ethyl acetate-water-formic acid (5: 1: 5: 0.01) and n-butanol-ethyl acetate-methanol-water-formic acid (5: 5: 1:9:0.05).
  
  
  RESULTS
  About 300 mg of crude extract was isolated by HSCCC, yielding 3.6 mg of scoulerine, 9.2 mg of isocorydine, 5.5 mg of dehydrocheilanthifoline, 7.5 mg of dehydrocavidine, 20.4 mg of palmatine and 20.9 mg of berberine, with purities of 71%, 92%, 85%, 76%, 90%, 97%, respectively.
  
  
  CONCLUSIONS
  It is time-saving and simple to isolate alkaloids from Corydalis saxicola by HSCCC with high yields and purities.
authors: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. xuanxuanch@gmail.com
authors: Xuan-xuan
authors: XX
authors: Cheng
authors: De-po
authors: DP
authors: Yang
authors: Dong-mei
authors: DM
authors: Wang
authors: Lin
authors: L
authors: Jiang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D001060:Aporphines / Q000302:isolation & purification; D001600:Berberine Alkaloids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031311:Corydalis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry
chemical_list: D000470:Alkaloids; D001060:Aporphines; D001600:Berberine Alkaloids; D012997:Solvents