title: Hepatitis B virus (HBV) genotypes in a group of Sri Lankan patients with chronic infection.
abstract: Hepatitis B infection causes a wide spectrum of liver diseases. Previous analyses   of hepatitis B virus (HBV) genome have revealed eight HBV genotypes (A-H), with distinct   geographical distribution worldwide. The epidemiology of HBV genotypes and their   implications for natural history of disease progression and response to anti viral therapy have   been increasingly recognized. This study was undertaken to determine the HBV genotypes in   a group of Sri Lankan patients with chronic infection who presented for investigation prior to   treatment. Genotypes were determined (2007-2009) in 25 patients with evidence of chronic   HBV infection. A genotyping system based on multiplex-nested PCR using type-specific primers   was employed in assigning genotypes A through F. Genotypes G and H were not determined.   Among the 25 patients tested, genotypes B [9 (36%)], C [4 (16%)], D [3 (12%)], A [2 (8%)] and   E [1 (4%)] were detected. There was a relatively high prevalence of mixed infections with   genotypes B+C (3), A+D (1), and B+D (2), which overall constituted 24% of patients. Although   this is a non-representative sample, HBV infections among this group of Sri Lankan patients   were predominantly genotypes B, C and D.
authors: Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka. areshamanamperi@gmail.com
authors: A
authors: A
authors: Manamperi
authors: N S
authors: NS
authors: Gunawardene
authors: C
authors: C
authors: Wellawatta
authors: W
authors: W
authors: Abeyewickreme
authors: H J
authors: HJ
authors: de Silva
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D017720:Molecular Epidemiology; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D013188:Sri Lanka / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral