title: Activity-dependent changes in cholinergic innervation of the mouse olfactory bulb.
abstract: The interplay between olfactory activity and cholinergic modulation remains to be fully understood. This report examines the pattern of cholinergic innervation throughout the murine main olfactory bulb across different developmental stages and in naris-occluded animals. To visualize the pattern of cholinergic innervation, we used a transgenic mouse model, which expresses a fusion of the microtubule-associated protein, tau, with green fluorescence protein (GFP) under the control of the choline acetyltransferase (ChAT) promoter. This tau-GFP fusion product allows for a remarkably vivid and clear visualization of cholinergic innervation in the main olfactory bulb (MOB). Interestingly, we find an uneven distribution of GFP label in the adult glomerular layer (GL), where anterior, medial, and lateral glomerular regions of the bulb receive relatively heavier cholinergic innervation than other regions. In contrast to previous reports, we find a marked change in the pattern of cholinergic innervation to the GL following unilateral naris occlusion between the ipsilateral and contralateral bulbs in adult animals.
authors: Department of Cell and Developmental Biology, University of Colorado, School of Medicine, Aurora, Colorado, United States of America.
authors: Ernesto
authors: E
authors: Salcedo
authors: Tuan
authors: T
authors: Tran
authors: Xuan
authors: X
authors: Ly
authors: Robert
authors: R
authors: Lopez
authors: Cortney
authors: C
authors: Barbica
authors: Diego
authors: D
authors: Restrepo
authors: Sukumar
authors: S
authors: Vijayaraghavan
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D059329:Cholinergic Neurons / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D009830:Olfactory Bulb / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D049452:Green Fluorescent Proteins; D002795:Choline O-Acetyltransferase