title: Dynamic SERS imaging of cellular transport pathways with endocytosed gold nanoparticles.
abstract: Dynamic SERS imaging inside a living cell is demonstrated with the use of a gold nanoparticle, which travels through the intracellular space to probe local molecular information over time. Simultaneous tracking of particle motion and SERS spectroscopy allows us to detect intracellular molecules at 65 nm spatial resolution and 50 ms temporal resolution, providing molecular maps of organelle transport and lisosomal accumulation. Multiplex spectral and trajectory imaging will enable imaging of specific dynamic biological functions such as membrane protein diffusion, nuclear entry, and rearrangement of cellular cytoskeleton.
authors: Department of Applied Physics and Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Jun
authors: J
authors: Ando
authors: Katsumasa
authors: K
authors: Fujita
authors: Nicholas I
authors: NI
authors: Smith
authors: Satoshi
authors: S
authors: Kawata
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D004705:Endocytosis; D006046:Gold / Q000032:analysis* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D006046:Gold