title: The bovine CXCR1 gene is highly polymorphic.
abstract: Several single nucleotide polymorphisms (SNP) in the bovine CXCR1 gene have been implicated in resistance to mastitis and milk somatic cell counts in several sample populations of Holstein dairy cows. As such, a more thorough understanding of SNP present in and near the bovine CXCR1 gene is needed. This study identified 36 SNP in the coding region and surrounding sequences of CXCR1 in 88 Holstein dairy cows. Four SNP induced amino acid changes and 1 SNP an early stop codon. Two amino acid changes occur in the third intracellular loop and C-terminus in locations tied to intracellular signaling. The 36 SNP could be subdivided into 4 separate linkage groups. Using representative or 'tag' SNP from each linkage group, haplotypes or the combination of SNP found on a single allele were generated to increase the specificity of an animal's genetic background. Four haplotypes were identified that represented 99% of the sample population. The haplotypes generated using tag SNP agreed with haplotypes generated from SNP causing amino acid changes. In conclusion, the CXCR1 gene is highly polymorphic and has potential implications towards genetic selection and understanding host factors that increase the risk of infection.
authors: The University of Tennessee, Department of Animal Science, 104 McCord Hall, 2640 Morgan Circle, Knoxville, TN 37996, USA. pighetti@utk.edu
authors: Gina M
authors: GM
authors: Pighetti
authors: Cheryl J
authors: CJ
authors: Kojima
authors: Leszek
authors: L
authors: Wojakiewicz
authors: Magdalena
authors: M
authors: Rambeaud
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics / Q000276:immunology; D008040:Genetic Linkage / Q000235:genetics; D006239:Haplotypes / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D023062:Receptors, Interleukin-8A / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D023062:Receptors, Interleukin-8A