title: [Construction and biological assessment of tissue engineering intervertebral disc].
abstract: OBJECTIVE
  To investigate the value of bone marrow-mesenchymal stem cells (BM-MSCs) transformed by nucleus pulposus (NPs) for construction of tissue engineering disc.
  
  
  METHODS
  BM-MSCs and fetal NPs were cultured in vitro, planted on polylactic acid-polyglycolic acid copolymer (PLGA), and observed with inverted microscope and scanning electronic microscope. PLGA scaffolds with adherent BM-MSCs and NPs, as well as BM-MSCs and NPs suspension were implanted into intervertebral discs of New Zealand white rabbits, respectively. Intervertebral signal intensity was evaluated by Thompson grading 12 weeks later. Proteoglycan and type IIcollagen were determined by spectrophotometric method and immunohistochemistry, respectively.
  
  
  RESULTS
  Spindle or multi-angular BM-MSCs turned into fibro-like phenotype coculture of BM-MSCs and NPs, which grew well with normal morphology when they attached on PLGA scaffolds. There was statistical difference in intervertebral signal intensity, and the expression of proteoglycan and type IIcollagen between PLGA scaffolds group and control group (P < 0.05), the content of proteoglycan was (3.93 ± 0.31) mg/100 mg in the PLGA scaffolds group whereas (3.52 ± 0.26) mg/100 mg in the control group.
  
  
  CONCLUSIONS
  BM-MSCs can be induced into NPs by cocultivation, and PLGA scaffolds can provide good growing conditions, and maintain high mechanical properties and spacial structure which meet the requirement of tissue engineering disc to prevent degeneration.
authors: Department of Spinal Surgery, Affiliated Hospital of Qingdao University Medical College, China.
authors: Hong-Fei
authors: HF
authors: Xiang
authors: Bin
authors: B
authors: Yue
authors: Xue-Xiao
authors: XX
authors: Ma
authors: Guo-Qing
authors: GQ
authors: Zhang
authors: Quan-Xiu
authors: QX
authors: Li
authors: Yue-Qiu
authors: YQ
authors: Wang
authors: Bo-Hua
authors: BH
authors: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007403:Intervertebral Disc / Q000166:cytology*; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid