title: Developmental changes and regional localization of Dspp, Mepe, Mimecan and Versican in postnatal developing mouse teeth.
abstract: It has been implicated noncollagenous proteins act as important regulators during odontogenesis. To test the hypothesis that the roles of Dspp, Mepe, Versican and Mimecan in the regulation of odontogenesis may be complementary, comparative investigations on the localization of four proteins were performed by immunohistochemical staining using mouse first molar at different developmental stages as a model. In postnatal 1- day-old mice, all the proteins, excluding Mepe, showed co-expression in young odontoblasts. At postnatal 3, strong immunoreactions for all proteins were detected in odontoblasts. Interestingly, Mepe was present within both cytoplasm and nucleus in odontoblasts. In mice older than 5 days, the expression of Dspp, Mimecan and Versican accumulated in subodontoblastic layer of the coronal pulp at high levels while the co-expression of Mepe and Mimecan significantly existed in predentin. The temporal-spatial specific pattern and unique co-localization of Dspp, Mepe, Mimecan and Versican suggest they play complementary roles during odontogenesis.
authors: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School of Stomatology Shandong University, 44-1# West Wenhua Road, Jinan, 250012, Shandong, People's Republic of China.
authors: C
authors: C
authors: Hou
authors: Z X
authors: ZX
authors: Liu
authors: K L
authors: KL
authors: Tang
authors: M G
authors: MG
authors: Wang
authors: J
authors: J
authors: Sun
authors: J
authors: J
authors: Wang
authors: S
authors: S
authors: Li
mesh_terms: D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009805:Odontogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism*; D014070:Tooth / Q000254:growth & development / Q000378:metabolism*; D053675:Versicans / Q000235:genetics / Q000378:metabolism*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D053675:Versicans; D000099311:Dentin Sialophosphoprotein; C518716:Mepe protein, mouse; C512906:Ogn protein, mouse; C508323:Vcan protein, mouse