title: [Inhibitory effects of Gecko alcohol extract on human esophageal squamous carcinoma cell line EC-109 proliferation and associated mechanism].
abstract: OBJECTIVE
  To explore the proliferation inhibition effects of Gecko alcohol extract (GAE) on human esophageal squamous carcinoma cell line EC-109 and its mechanism.
  
  
  METHODS
  The inhibitory effects of GAE on proliferation of EC-109 cells were measured by MTT. Nucleolus change of apoptotic cells was observed by Hoechest33342 fluorescence staining. Apoptosis rate of EC-109 cells was detected by flow cytometry. The expressions of apoptosis protein Caspase-3 and FAS in EC-109 cells were investigated by immunohistochemistry.
  
  
  RESULTS
  GAE had the inhibition effects on the proliferation of esophageal carcinoma cell EC-109. The apoptosis rate of EC-109 cell treated with GAE(3.0 mg/mL, 4.0 mg/mL) for 48h was 20.63% and 39.73%, respectively. Compared with control group,the expression of Fas and Caspase-3 was significantly up-regulated in GAE treated group.
  
  
  CONCLUSIONS
  GAE can inhibit the proliferation of esophageal carcinoma EC-109 cells and induce them apoptosis which may be correlated with increasing expression of protein Fas and Caspase-3.
authors: Medical College, Henan University of Science and Technology, Luoyang 471003, China. 2007songjiayu@163.com
authors: Jia-yu
authors: JY
authors: Song
authors: Xiao-lan
authors: XL
authors: Wang
authors: Jian-gang
authors: JG
authors: Wang
authors: Shou-min
authors: SM
authors: Xi
authors: Ling
authors: L
authors: Liu
authors: Rui-fang
authors: RF
authors: Li
authors: Kai
authors: K
authors: Yu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004938:Esophageal Neoplasms / Q000473:pathology; D000431:Ethanol / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008116:Lizards; D008419:Materia Medica / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C491901:FAS protein, human; D008419:Materia Medica; D019014:fas Receptor; D000431:Ethanol; C505407:CASP3 protein, human; D053148:Caspase 3