title: Chemical-physical properties and in vitro cell culturing of a novel biphasic bio-mimetic scaffold for osteo-chondral tissue regeneration.
abstract: The requirements for a successful regeneration of an osteo-chondral defect could effectively be met by using a bi-layered composite scaffold, able to support proliferation and differentiation of mesenchymal stem cells, while providing a biochemical environment promoting the formations of the two distinct tissues. The novel strategy here presented consists of developing a bio-mimetic scaffolds obtained by the combination of two integrated organic compounds (type I collagen and chitosan) with or without bioactive Mg-doped hydroxyapatite (Mg-HA) nanocrystals, depending on the specific layer, reproducing cartilaginous or subchondral bone tissue. An innovative patented methodology for scaffolds production, called - pH-dependent 3-phasic assembling -, allowed to development of a highly homogenous and chemically stable scaffold, presenting a very good integration among all three components, as confirmed by extensive SEM and thermogravimetric analyses. A preliminary in vitro evaluation was also carried out by seeding bi-layered scaffold with human bone marrow stromal cells (h-MSCs), by giving particular emphasis to cell viability and distribution at day 0, 7 and 14. Cells were viable and uniformly colonized the whole scaffold until day 14, indicating that the scaffold contributed to the maintenance of cell behaviour.
authors: SC Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, Istituto Ortopedico Rizzoli, Bologna, Italy.
authors: B
authors: B
authors: Grigolo
authors: M
authors: M
authors: Fiorini
authors: C
authors: C
authors: Manferdini
authors: C
authors: C
authors: Cavallo
authors: E
authors: E
authors: Gabusi
authors: N
authors: N
authors: Zini
authors: L
authors: L
authors: Dolcini
authors: A
authors: A
authors: Nicoletti
authors: D
authors: D
authors: Pressato
authors: A
authors: A
authors: Facchini
authors: G
authors: G
authors: Lisignoli
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry; D002356:Cartilage; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D024042:Collagen Type I; D048271:Chitosan; D017886:Durapatite