title: Combined expression of BUB1B, DLGAP5, and PINK1 as predictors of poor outcome in adrenocortical tumors: validation in a Brazilian cohort of adult and pediatric patients.
abstract: BACKGROUND
  A recent microarray study identified a set of genes whose combined expression patterns were predictive of poor outcome in a cohort of adult adrenocortical tumors (ACTs). The difference between the expression values measured by qRT-PCR of DLGAP5 and PINK1 genes was the best molecular predictor of recurrence and malignancy. Among the adrenocortical carcinomas, the combined expression of BUB1B and PINK1 genes was the most reliable predictor of overall survival. The prognostic and molecular heterogeneity of ACTs raises the need to study the applicability of these molecular markers in other cohorts.
  
  
  OBJECTIVE
  To validate the combined expression of BUB1B, DLGAP5, and PINK1 as outcome predictor in ACTs from a Brazilian cohort of adult and pediatric patients.
  
  
  METHODS
  BUB1B, DLGAP5, and PINK1 expression was assessed by quantitative PCR in 53 ACTs from 52 patients - 24 pediatric and 28 adults (one pediatric patient presented a bilateral asynchronous ACT).
  
  
  RESULTS
  DLGAP5-PINK1 and BUB1B-PINK1 were strong predictors of disease-free survival and overall survival, respectively, among adult patients with ACT. In the pediatric cohort, these molecular predictors were only marginally associated with disease-free survival but not with overall survival.
  
  
  CONCLUSIONS
  This study confirms the prognostic value of the combined expression of BUB1B, DLGAP5, and PINK1 genes in a Brazilian group of adult ACTs. Among pediatric ACTs, other molecular predictors of outcome are required.
authors: Unidade de Suprarrenal, Laboratório de Hormônios e Genética Molecular/LIM42 da Disciplina de Endocrinologie Metabologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Avenida Dr Eneas de Carvalho Aguiar 155, 20 andar, Bloco 6, CEP: 05403900 São Paulo, Brazil. mariafragoso@uol.com.br
authors: Maria Candida B V
authors: MC
authors: Fragoso
authors: Madson Queiroz
authors: MQ
authors: Almeida
authors: Tania L
authors: TL
authors: Mazzuco
authors: Beatriz M P
authors: BM
authors: Mariani
authors: Luciana P
authors: LP
authors: Brito
authors: Talita Cardoso
authors: TC
authors: Gonçalves
authors: Guilherme A
authors: GA
authors: Alencar
authors: Lorena de O
authors: Lde O
authors: Lima
authors: Andre M
authors: AM
authors: Faria
authors: Isabelle
authors: I
authors: Bourdeau
authors: Antonio M
authors: AM
authors: Lucon
authors: Daniel S
authors: DS
authors: Freire
authors: Ana Claudia
authors: AC
authors: Latronico
authors: Berenice B
authors: BB
authors: Mendonca
authors: Andre
authors: A
authors: Lacroix
authors: Antonio M
authors: AM
authors: Lerario
mesh_terms: D000293:Adolescent; D000306:Adrenal Cortex Neoplasms / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D000099202:PTEN-Induced Putative Kinase
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D000099202:PTEN-Induced Putative Kinase; C465041:DLGAP5 protein, human; C090464:Bub1 spindle checkpoint protein