title: [Effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates].
abstract: OBJECTIVE
  To investigate the effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates (CSC).
  
  
  METHODS
  Both IL-8mNF-kappaB(-) BEAS-2B cells (BEAS-2B cells with mutated NF-kappaB binding site in IL-8 promoter) and IL-8 WT BEAS-2B cells (wild-type BEAS-2B cells with completed NF-kappaB binding site in IL-8 promoter) were treated by 7.5% CSC. The mRNA level of fLCF gene was detected by real-time PCR, and the activity of luciferase was measured by luciferase reporter gene activity detection kit.
  
  
  RESULTS
  After two kinds of BEAS-2B cells were treated by 7.5% CSC, the mRNA level of fLCF and the activity of luciferase in IL-8 WT BEAS-2B cells was higher than those in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001), the mRNA level of fLCF in IL-8 WT BEAS-2B cells was 1.32 times as that in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001).
  
  
  CONCLUSIONS
  The results suggested that NF-kappaB transcription factor was involved in smoking-induced respiratory inflammation through the precise molecular mechanisms of promoting the release of IL-8 in BEAS-2B cells treated by CSC.
authors: Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. zhangqiao@zzu.edu.cn
authors: Qiao
authors: Q
authors: Zhang
authors: Luming
authors: L
authors: Cheng
authors: Ruiting
authors: R
authors: Xing
authors: Lu
authors: L
authors: Ma
authors: Chunyang
authors: C
authors: Li
authors: Yubao
authors: Y
authors: Xu
authors: Weidong
authors: W
authors: Wu
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects
chemical_list: D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D012906:Smoke; D014028:Tobacco Smoke Pollution; D008156:Luciferases