title: Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica.
abstract: The tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup efficiency, which was ascribed to the increasing affinity of the tip to the DNA molecules along with the increase of the tip radius after wearing. It was demonstrated that bare mica was superior to APTES-modified mica to keep the tip clean while wearing, which was crucial for DNA pickup during AFM nanomanipulation.
authors: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China.
authors: Na
authors: N
authors: Duan
authors: Fei
authors: F
authors: Long
authors: Xinyan
authors: X
authors: Wang
authors: Bin
authors: B
authors: Li
authors: Jun
authors: J
authors: Hu
authors: Yi
authors: Y
authors: Zhang
mesh_terms: D000538:Aluminum Silicates / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D018625:Microscopy, Atomic Force / Q000379:methods*
chemical_list: D000538:Aluminum Silicates; D004247:DNA; C011934:mica