title: [Study of molecular mechanisms of fuyuan capsule, icariin and arasaponin R1 in treatment of osteoarthritis].
abstract: OBJECTIVE
  To study the molecular mechanisms of Fuyuan capsule serum containing, icariin and arasaponin R1 in the treatment of osteoarthritis from the urokinase-type plasminogen activator (uPA) system.
  
  
  METHODS
  Chondrocytes were isolated, cultured and identified using type II collagens immunostaining. After stimulating with TNF-alpha 10 microg x L(-1), 1 h, then the chondrocytes were treatment with glucosamine hydrochloride 25 g x L(-1), 20% Fuyuan capsule serum containing, icariin 12.5 mg x L(-1), arasaponin R1 125 mg x L(-1), icariin 12.5 mg x L(-1) + arasaponin R1 125 mg x L(-1). After 2 h, expression of uPA and nuclear factor kappa B (NF-kappaB P65) mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR), the activities of NF-kappaB(P65) combine DNA were determined by electrophoretic mobility shift assays (EMSA), 1kappaBalpha were detected by Western blotting.
  
  
  RESULTS
  Fuyuan capsule, icariin and arasaponin R1 could significantly reduce NF-kappaB (P65) activities and uPA mRNA expression, and increase expression of IkappaBalpha (P < 0.01), but no significant difference between all treatment groups.
  
  
  CONCLUSIONS
  Fuyuan capsule and its two main active ingredients, icariin and arasaponin R1, could protect chondrocytes from damage through reducing the NF-kappaB (P65) activities, increasing the express of IkappaBalpha and then reducing uPA of chondrocytes.
authors: First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. zhangwl6996@sina.com
authors: Wenliang
authors: W
authors: Zhang
authors: Rongheng
authors: R
authors: Li
authors: Shumei
authors: S
authors: Wang
authors: Xiaoli
authors: X
authors: Zhou
authors: Yu
authors: Y
authors: Zhong
mesh_terms: D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005786:Gene Expression Regulation; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism
chemical_list: D002214:Capsules; D004268:DNA-Binding Proteins; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D016328:NF-kappa B; D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator; C056599:icariin