title: Anti-fouling surfaces by combined molecular self-assembly and surface-initiated ATRP for micropatterning active proteins.
abstract: A simple method by combined molecular self assembly and surface-initiated atom transfer radical polymerization (SI-ATRP) was proposed to prepare a biologically inert surface for micropatterning active proteins. The MPEG microdomains having a short terminal poly(ethylene glycol) (PEG) unit were prepared by self assembly of 2-(methyoxy(polyethylenoxy) propyl)trimethoxy silane (MPEG-silane). The remaining local regions or poly(poly(ethylene glycol)methyl ether methacrylate-co-glycidyl methacrylate) (P(PEGMEMA-co-GMA)) microdomains were produced via SI-ATRP of PEGMEMA and GMA comonomers. The epoxy groups of the P(PEGMEMA-co-GMA) microdomains were used directly for covalent coupling of an active protein (human immunoglobulin or IgG) via the ring-opening reaction to produce the IgG-coupled microdomains. The IgG-coupled microdomains interact only and specifically with target anti-IgG, while the other antifouling microregions from self-assembled monolayers with short terminal PEG units effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored.
authors: State Key Laboratory of Chemical Resource Engineering, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing, China.
authors: K M
authors: KM
authors: Xiu
authors: Q
authors: Q
authors: Cai
authors: J S
authors: JS
authors: Li
authors: X P
authors: XP
authors: Yang
authors: W T
authors: WT
authors: Yang
authors: F J
authors: FJ
authors: Xu
mesh_terms: D000327:Adsorption; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D004202:Disinfectants / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D018625:Microscopy, Atomic Force; D025202:Molecular Diagnostic Techniques; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D059629:Signal-To-Noise Ratio; D012825:Silicon / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
chemical_list: D000888:Antibodies, Anti-Idiotypic; D004202:Disinfectants; D005609:Free Radicals; D055767:Immobilized Proteins; D007074:Immunoglobulin G; D011109:Polymethacrylic Acids; C048844:anti-IgG; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D012825:Silicon