title: Effects of different extraction buffers on peanut protein detectability and lateral flow device (LFD) performance.
abstract: The accidental uptake of peanuts can cause severe health reactions in allergic individuals. Reliable determination of traces of peanuts in food products is required to support correct labelling and therefore minimise consumers' risk. The immunoanalytical detectability of potentially allergenic peanut proteins is dependent on previous heat treatment, the extraction capacity of the applied buffer and the specificity of the antibody. In this study a lateral flow device (LFD) for the detection of peanut protein was developed and the capacity of 30 different buffers to extract proteins from mildly and strongly roasted peanut samples as well as their influence on the test strip performance were investigated. Most of the tested buffers showed good extraction capacity for putative Ara h 1 from mildly roasted peanuts. Protein extraction from dark-roasted samples required denaturing additives, which were proven to be incompatible with LFD performance. High-pH buffers increased the protein yield but inhibited signal generation on the test strip. Overall, the best results were achieved using neutral phosphate buffers but equal detectability of differently altered proteins due to food processing cannot be assured yet for immunoanalytical methods.
authors: Christian Doppler Laboratory for Rapid Test Systems for Allergenic Food Contaminants, University of Natural Resources and Life Sciences, Vienna, Austria.
authors: J
authors: J
authors: Rudolf
authors: P
authors: P
authors: Ansari
authors: C
authors: C
authors: Kern
authors: T
authors: T
authors: Ludwig
authors: S
authors: S
authors: Baumgartner
mesh_terms: D000918:Antibody Specificity; D010367:Arachis / Q000737:chemistry*; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel; D010940:Plant Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D002021:Buffers; D010940:Plant Proteins