title: Silencing of GSTP1, a prostate cancer prognostic gene, by the estrogen receptor-β and endothelial nitric oxide synthase complex.
abstract: We recently identified in prostate tumors (PCa) a transcriptional prognostic signature comprising a significant number of genes differentially regulated in patients with worse clinical outcome. Induction of up-regulated genes was due to chromatin remodeling by a combinatorial complex between estrogen receptor (ER)-β and endothelial nitric oxide synthase (eNOS). Here we show that this complex can also repress transcription of prognostic genes that are down-regulated in PCa, such as the glutathione transferase gene GSTP1. Silencing of GSTP1 is a common early event in prostate carcinogenesis, frequently caused by promoter hypermethylation. We validated loss of glutathione transferase (GST) P1-1 expression in vivo, in tissue microarrays from a retrospective cohort of patients, and correlated it with decreased disease-specific survival. Furthermore, we show that in PCa cultured cells ERβ/eNOS causes GSTP1 repression by being recruited at estrogen responsive elements in the gene promoter with consequential remodeling of local chromatin. Treatment with ERβ antagonist or its natural ligand 5α-androstane-3β,17β-diol, eNOS inhibitors or ERβ small interference RNA abrogated the binding and reversed GSTP1 silencing, demonstrating the direct involvement of the complex. In vitro, GSTP1 silencing by ERβ/eNOS was specific for cells from patients with worse clinical outcome where it appeared the sole mechanism regulating GSTP1 expression because no promoter hypermethylation was present. However, in vivo chromatin immunoprecipitation assays on fresh PCa tissues demonstrated that silencing by ERβ/eNOS can coexist with promoter hypermethylation. Our findings reveal that the ERβ/eNOS complex can exert transcriptional repression and suggest that this may represent an epigenetic event favoring inactivation of the GSTP1 locus by methylation. Moreover, abrogation of ERβ/eNOS function by 3β-adiol emphasizes the significance of circulating or locally produced sex steroid hormones or their metabolites in PCa biology with relevant clinical/therapeutic implications.
authors: Regina Elena Cancer Institute, 00158 Rome, Italy.
authors: A
authors: A
authors: Re
authors: A
authors: A
authors: Aiello
authors: S
authors: S
authors: Nanni
authors: A
authors: A
authors: Grasselli
authors: V
authors: V
authors: Benvenuti
authors: V
authors: V
authors: Pantisano
authors: L
authors: L
authors: Strigari
authors: C
authors: C
authors: Colussi
authors: S
authors: S
authors: Ciccone
authors: A P
authors: AP
authors: Mazzetti
authors: F
authors: F
authors: Pierconti
authors: F
authors: F
authors: Pinto
authors: P
authors: P
authors: Bassi
authors: M
authors: M
authors: Gallucci
authors: S
authors: S
authors: Sentinelli
authors: F
authors: F
authors: Trimarchi
authors: S
authors: S
authors: Bacchetti
authors: A
authors: A
authors: Pontecorvi
authors: M
authors: M
authors: Lo Bello
authors: A
authors: A
authors: Farsetti
mesh_terms: D015113:Androstane-3,17-diol / Q000494:pharmacology / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement; D042002:Chromatin Assembly and Disassembly; D019175:DNA Methylation; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology; D047629:Estrogen Receptor beta / Q000819:agonists / Q000378:metabolism*; D020868:Gene Silencing; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D011379:Prognosis; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D046888:Tissue Array Analysis; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D047629:Estrogen Receptor beta; D015113:Androstane-3,17-diol; D004958:Estradiol; D052250:Nitric Oxide Synthase Type III; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi