title: Polyclonal antibodies against protein F from the cell envelope of Pseudomonas fluorescens for the detection of psychrotrophic bacteria in refrigerated meat using an indirect ELISA.
abstract: An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for detection of Pseudomonas fluorescens and related psychrotrophic bacteria in refrigerated meat. The ELISA uses polyclonal antibodies raised in rabbits against protein F from the cell envelope of Pseudomonas fluorescens AH-70. The anti-protein F antibodies were recovered from the crude antiserum by ammonium sulfate precipitation and conjugated to biotin. Commercial Extr Avidin-peroxidase conjugate was used to detect the biotinylated antibodies bound to their specific antigens. Subsequent enzymatic conversion of substrate gave distinct absorbance differences when assaying meat samples containing P. fluorescens strains of different origin as well as related psychrotrophic microorganisms. The detection threshold for the ELISA assay developed in this work was 10(4)-10(5) cfu cm(-2).
authors: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040, Madrid, Spain.
authors: I
authors: I
authors: González
authors: R
authors: R
authors: Martín
authors: T
authors: T
authors: García
authors: P
authors: P
authors: Morales
authors: B
authors: B
authors: Sanz
authors: P E
authors: PE
authors: Hernández