title: Proteome profiling of the green sulfur bacterium Chlorobaculum tepidum by N-terminal proteomics.
abstract: In this study, we performed the first large-scale identification of N-terminal peptides from the green sulfur bacterium Chlorobaculum tepidum. Combined fractional diagonal chromatography (COFRADIC) was used to isolate protein N-terminal peptides from three different proteome preparations, and following LC-MS/MS analysis, over 621 different proteins were identified by their N-terminal peptides. Our data constitute the largest data set currently available for protein N-termini of prokaryotic photosynthetic organisms.
authors: Division of Biochemistry, Department of Chemistry, University of Crete, Voutes Heraklion, Greece.
authors: Kalliopi
authors: K
authors: Kouyianou
authors: Pieter-Jan
authors: PJ
authors: De Bock
authors: Niklaas
authors: N
authors: Colaert
authors: Antigoni
authors: A
authors: Nikolaki
authors: Aikaterini
authors: A
authors: Aktoudianaki
authors: Kris
authors: K
authors: Gevaert
authors: Georgios
authors: G
authors: Tsiotis
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D019414:Chlorobi / Q000378:metabolism*; D002853:Chromatography, Liquid; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D020539:Sequence Analysis, Protein; D013347:Subcellular Fractions; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D010446:Peptide Fragments; D020543:Proteome