title: Functional analysis of SGR4635-induced enhancement of pigmented antibiotic production in Streptomyces lividans.
abstract: The Gram-positive mycelium-producing bacterium Streptomyces undergoes complex morphological differentiation after autolytic degradation of the vegetative mycelium. Cell-wall breakdown during growth stimulates cell development and secondary metabolite production by Streptomyces. N-acetylglucosamine (GlcNAc) produced by cell-wall lysis acts as a signal molecule, triggering the production of secondary metabolites in S. coelicolor A3(2). Here, we report that introduction of multiple copies of the GlcNAc-internalizing gene (sgr4635, encoding nagE2) of S. griseus activates actinorhodin and undecylprodigiosin production during the late growth of S. lividans in the absence of GlcNAc. Furthermore, the repressor-type transcriptional regulator DasR binds to two operator sites upstream of sgr4635. Our findings indicate that sgr4635 induces DasR-mediated antibiotic production by internalizing the GlcNAc accumulated from cell-wall lysis.
authors: Department of Biological Science, Myongji University, Gyeonggi-do, 449-728, Republic of Korea.
authors: Won-Jae
authors: WJ
authors: Chi
authors: Soon-Youl
authors: SY
authors: Lee
authors: Jaehag
authors: J
authors: Lee
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics*; D048170:Streptomyces lividans / Q000235:genetics* / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000117:Acetylglucosamine; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins; D011353:Prodigiosin; D011994:Recombinant Proteins; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones