title: A public platform for the verification of the phenotypic effect of candidate genes for resistance to aflatoxin accumulation and Aspergillus flavus infection in maize.
abstract: A public candidate gene testing pipeline for resistance to aflatoxin accumulation or Aspergillus flavus infection in maize is presented here. The pipeline consists of steps for identifying, testing, and verifying the association of selected maize gene sequences with resistance under field conditions. Resources include a database of genetic and protein sequences associated with the reduction in aflatoxin contamination from previous studies; eight diverse inbred maize lines for polymorphism identification within any maize gene sequence; four Quantitative Trait Loci (QTL) mapping populations and one association mapping panel, all phenotyped for aflatoxin accumulation resistance and associated phenotypes; and capacity for Insertion/Deletion (InDel) and SNP genotyping in the population(s) for mapping. To date, ten genes have been identified as possible candidate genes and put through the candidate gene testing pipeline, and results are presented here to demonstrate the utility of the pipeline.
authors: Corn Host Plant Resistance Research Unit, U.S. Department of Agriculture-Agricultural Research Service, MS 39762, USA. Marilyn.Warburton@ars.usda.gov
authors: Marilyn L
authors: ML
authors: Warburton
authors: William Paul
authors: WP
authors: Williams
authors: Leigh
authors: L
authors: Hawkins
authors: Susan
authors: S
authors: Bridges
authors: Cathy
authors: C
authors: Gresham
authors: Jonathan
authors: J
authors: Harper
authors: Seval
authors: S
authors: Ozkan
authors: J Erik
authors: JE
authors: Mylroie
authors: Xueyan
authors: X
authors: Shan
mesh_terms: D000348:Aflatoxins / Q000378:metabolism*; D001231:Aspergillus flavus / Q000378:metabolism / Q000472:pathogenicity; D001483:Base Sequence; D030541:Databases, Genetic; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D010641:Phenotype; D057865:Plant Immunity / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D003313:Zea mays / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D000348:Aflatoxins; D005819:Genetic Markers