title: Does oxidative stress affect the activity of the sodium-proton exchanger?
abstract: BACKGROUND
  Accumulation of reactive oxygen species (ROS) takes place in patients with chronic renal failure (CRF). Oxidative stress causes disorders in the activity of the sodium-proton exchanger (NHE). Studies on NHE in CRF produced results that are discrepant and difficult to interpret. The aim of this study was to demonstrate that oxidative stress had an effect on the activity of NHE.
  
  
  METHODS
  We enrolled 87 subjects divided into 4 groups: patients with CRF treated conservatively; patients with CRF hemodialyzed without glucose--HD-g(-); patients with CRF hemodialyzed with glucose--HD-g(+); controls (C). The activity of NHE, the rate of proton efflux V(max), Michaelis constant (Km), and the concentration of thiobarbituric acid-reactive substances (TBARS, an indicator of oxidative stress) in plasma, as well as the concentration of reduced glutathione in blood were determined.
  
  
  RESULTS
  The concentration of TBARS was significantly higher in hemodialyzed patients before and after dialysis and in patients with CRF on conservative treatment in comparison with group C. TBARS in plasma correlated negatively with VpH(i)6.4 in group C and with V(max) and VpH(i)6.4 after HD in group HD-g(-). We found that the concentration of creatinine correlated with TBARS (p < 0.0001; r = +0.51) in the conservatively treated group.
  
  
  CONCLUSIONS
  We observed a marked oxidative stress and decreased NHE activity when dialysis was done without glucose, whereas patients dialyzed with glucose demonstrated a relatively low intensity of oxidative stress.
authors: Zakład Chemii Medycznej, Pomorskiego Uniwersytetu Medycznego w Szczecinie, al. Powstańców Wlkp. 72, 70-111 Szczecin.
authors: Joanna
authors: J
authors: Bober
authors: Karolina
authors: K
authors: Kedzierska
authors: Ewa
authors: E
authors: Kwiatkowska
authors: Ewa
authors: E
authors: Stachowska
authors: Edyta
authors: E
authors: Gołembiewska
authors: Olech
authors: O
authors: Mazur
authors: Zdzisław
authors: Z
authors: Staniewicz
authors: Kazimierz
authors: K
authors: Ciechanowski
authors: Dariusz
authors: D
authors: Chlubek
mesh_terms: D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D015312:Hemodialysis Solutions / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000379:methods*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D015312:Hemodialysis Solutions; D017923:Sodium-Hydrogen Exchangers; D017392:Thiobarbituric Acid Reactive Substances; D005947:Glucose