title: Effect of exogenous administration of Candida albicans autoregulatory alcohols in a murine model of hematogenously disseminated candidiasis.
abstract: Candida albicans supernatants contain a mixture of autoregulatory alcohols. In vitro, when added individually or in combination, these alcohols inhibit the yeast to filamentous form conversion. Here we evaluate the in vivo effect of the exogenous administration of a Cocktail solution simulating the composition of alcohols present in a C. albicans culture supernatant (1 ml; 94 μmol l(-1) isoamyl alcohol, 70 μmol l(-1) 2-phenylethanol, 3.2 n mol l(-1) E -nerolidol, and 18 n mol l(-1) E,E -farnesol) using the well established murine model of hematogenously disseminated candidiasis. Mice injected intraperitoneally with the Cocktail solution demonstrated increased survival and decreased organ fungal burden compared to control mice. Histological observations suggest that the Cocktail, to some extent, has an inhibitory effect on cell filamentation within the kidney. These findings suggest that the exogenous administration of C. albicans autoregulatory alcohols displays a protective effect during disseminated candidiasis.
authors: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal.
authors: Margarida
authors: M
authors: Martins
authors: Anna L
authors: AL
authors: Lazzell
authors: Jose L
authors: JL
authors: Lopez-Ribot
authors: Mariana
authors: M
authors: Henriques
authors: Rosário
authors: R
authors: Oliveira
mesh_terms: D000438:Alcohols / Q000008:administration & dosage*; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D002176:Candida albicans / Q000187:drug effects* / Q000472:pathogenicity*; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D004195:Disease Models, Animal; D006131:Growth Inhibitors / Q000008:administration & dosage; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D051379:Mice; D016019:Survival Analysis
chemical_list: D000438:Alcohols; D000935:Antifungal Agents; D006131:Growth Inhibitors