title: Microsatellite loci in an endangered fern species, Athyrium viridescentipes (Woodsiaceae), and cross-species amplification.
abstract: OBJECTIVE
  Microsatellite markers were characterized in Athyrium viridescentipes, a critically endangered fern species in Japan, to investigate its genetic diversity and population structure.
  
  
  RESULTS
  Fifteen microsatellite markers were developed. The 15 loci were successfully amplified in three additional Athyrium species except for one locus in A. vidalii. In A. viridescentipes, the number of alleles per locus ranged from one to five, with an average of 1.9, and the expected heterozygosity ranged from 0.00 to 0.53, with an average of 0.24.
  
  
  CONCLUSIONS
  These markers can be used in studies on conservation programs for A. viridescentipes as well as in further studies involving other Athyrium species.
authors: Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan. ayako.i@ay5.ecs.kyoto-u.ac.jp
authors: Ayako
authors: A
authors: Izuno
authors: Masayuki
authors: M
authors: Takamiya
authors: Shingo
authors: S
authors: Kaneko
authors: Yuji
authors: Y
authors: Isagi
mesh_terms: D056727:Endangered Species; D029624:Ferns / Q000235:genetics*; D005784:Gene Amplification / Q000235:genetics*; D056426:Genetic Loci; D005828:Genetics, Population; D006579:Heterozygote; D007564:Japan; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013045:Species Specificity