title: Inhibition of trauma-associated inflammatory liver damage by blocking NF-κB activity.
abstract: OBJECTIVE
  NF-κB protein family members act as transcription facts and play a key role in regulating the immune response to infection and inflammatory signals. We proposed to determine the role of NF-κB in the development of trauma-associated liver damage and inflammation.
  
  
  METHODS
  NF-κB DNA-binding activity was inhibited using double-stranded oligodeoxynucleotides (ODN). A total of 288 Wistar rats were randomly divided into four groups: control (C), traumatic inflammation (T), traumatic inflammation plus NF-κB decoy (ODN) and traumatic inflammation plus mutant NF-κB decoy ODN (mODN).
  
  
  RESULTS
  Our data shows that inhibition of NF-κB activation significantly reduces liver tissue damage as evidenced by serum ALT levels and histological changes using both light microscopy and transmission electron microscopy. Furthermore, EMSA results showed that NF-κB activation was reduced in Group ODN rats compared to Group T and Group mODN rats. Expression of TNF-a and IL-6 protein in Group ODN rats were also reduced compared to Group T and Group mODN rats. We demonstrated that NF-κB plays an important role in trauma-associated inflammation and liver tissue damage.
  
  
  CONCLUSIONS
  Suppressing NF-κB activation effectively reduces the release of the pro-inflammatory cytokines TNF-a and IL-6 following liver trauma.
authors: Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors: Wenjun
authors: W
authors: Yang
authors: Yihu
authors: Y
authors: Zheng
authors: Han
authors: H
authors: He
authors: Huxiang
authors: H
authors: Zhang
authors: Li
authors: L
authors: Wan
authors: Qiyu
authors: Q
authors: Zhang
authors: Zhengping
authors: Z
authors: Yu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D007249:Inflammation / Q000209:etiology / Q000517:prevention & control; D015850:Interleukin-6 / Q000097:blood; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000209:etiology* / Q000517:prevention & control; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014947:Wounds and Injuries / Q000150:complications*
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase