title: [Study on the virus-free technology of Pinellia temata].
abstract: OBJECTIVE
  To solve the degradation of production and quality of Pinellia caused by the virus accumulation, rapid propagation technical of virus-free Pinellia was researched.
  
  
  METHODS
  Pinellia leaves,petioles as explants, technology of using high temperature (38 degrees C, 40d) and shoot tip culture producing virus-free Pinellia was explored.
  
  
  RESULTS
  The results showed that leaves without virus spots was about 88.9% when explants were culture for 40d at high temperature (38 degrees C). 1.0 mg/L 6-BA and 0.1 mg/L NAA could induce seedling from shoot tip,seedling rate is up to 91.4%; MS added 0.5 mg/L 6-BA and 0.1 mg/L NAA was conducive to growth of the plantlets; added 0.5 mg/L KT and 0.5 mg/L NAA was in favor of inducing root and promoting root growth, the survival rate of the transplanting seedling could reach 89.5%.
  
  
  CONCLUSIONS
  A reliable system of virus-free Pinellia propagation is established.
authors: Cotton Research Institute, Shanxi Academy of Agricultural Science, Yuncheng 044000, China. xxhwzq@126.com
authors: Xiao-hong
authors: XH
authors: Xie
authors: Jiang-hui
authors: JH
authors: Li
authors: Li
authors: L
authors: Chen
authors: Bo
authors: B
authors: Li
authors: Lei
authors: L
authors: Pei
authors: Jian-chun
authors: JC
authors: Fan
authors: Hong-e
authors: HE
authors: Xie
authors: Zong-xin
authors: ZX
authors: Wu
mesh_terms: D003470:Culture Media; D006358:Hot Temperature; D030021:Pinellia / Q000187:drug effects / Q000254:growth & development*; D010935:Plant Diseases / Q000517:prevention & control* / Q000821:virology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators