title: Production of conjugated linoleic acids by Lactobacillus plantarum strains isolated from naturally fermented Chinese pickles.
abstract: Naturally fermented pickles harbour many lactic acid bacteria (LAB). Forty-three LAB strains with conjugated linoleic acid (CLA)-producing ability were isolated from three naturally fermented pickle brines. Of these isolates, lp15 identified as Lactobacillus plantarum by API 50 CHL system and full-length 16S rDNA sequence analysis exhibited the highest CLA-producing ability (26.1% conversion) at 48 h in de Man Rogosa Sharpe (MRS) broth in the presence of 100 µg/ml of linoleic acid (LA). Compared to other strains, L. plantarum strain lp15 showed the highest tolerance upon increased levels of LA in the medium, i.e., up to 600 µg/ml. This strain converted about 25% of LA into CLA isomers [predominantly cis-9, trans-11 CLA (9-CLA) and trans-10, cis-12 CLA (10-CLA)], of which 75% was 9-CLA. Interestingly, though the conversion rate of LA into CLA by lp15 remained stable between 100 to 600 µg/ml LA levels in the medium, it dropped sharply at 1000 µg/ml. Taken together, the lp15 strain displayed relatively high LA tolerance with higher conversion rate, which implies that this strain is a valuable candidate for enhancing the CLA content in food-sources like pickles.
authors: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors: Pei
authors: P
authors: Liu
authors: Sheng-Rong
authors: SR
authors: Shen
authors: Hui
authors: H
authors: Ruan
authors: Qian
authors: Q
authors: Zhou
authors: Liu-Liu
authors: LL
authors: Ma
authors: Guo-Qing
authors: GQ
authors: He
mesh_terms: D018553:Cucumis sativus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D044243:Linoleic Acids, Conjugated; D012336:RNA, Ribosomal, 16S