title: A method for analysis of lipid vesicle domain structure from confocal image data.
abstract: Quantitative characterization of the lateral structure of curved membranes based on fluorescence microscopy requires knowledge of the fluorophore distribution on the surface. We present an image analysis approach for extraction of the fluorophore distribution on a spherical lipid vesicle from confocal imaging stacks. The technique involves projection of volumetric image data onto a triangulated surface mesh representation of the membrane, correction of photoselection effects and global motion of the vesicle during image acquisition and segmentation of the surface into domains using histograms. The analysis allows for investigation of the morphology and size distribution of domains on the surface.
authors: Department of Physics and Chemistry, MEMPHYS Centre for Biomembrane Physics, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark.
authors: Peter
authors: P
authors: Husen
authors: Matthias
authors: M
authors: Fidorra
authors: Steffen
authors: S
authors: Härtel
authors: Luis A
authors: LA
authors: Bagatolli
authors: John H
authors: JH
authors: Ipsen
mesh_terms: D018613:Microscopy, Confocal / Q000379:methods*; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D053835:Unilamellar Liposomes