title: Steroid hormones analysis with surface-assisted laser desorption/ionization mass spectrometry using catechin-modified titanium dioxide nanoparticles.
abstract: This paper describes the application of catechin-modified titanium dioxide nanoparticles (TiO(2) NPs) as matrices to analyze four steroid hormones by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The catechin-modified TiO(2) NPs have high absorbance at 337 nm and are effective SALDI matrices when using a nitrogen laser. Four test steroid hormones-cortisone, hydrocortisone, progesterone, and testosterone-were directly analyzed by SALDI-MS. The limits of detection at a signal-to-noise ratio of 3 for cortisone, hydrocortisone, progesterone, and testosterone were 1.62, 0.70, 0.66, and 0.23 μM, respectively. This approach provides good quantitative linearity for the four analytes (R(2)>0.986) with good reproducibility (the shot-to-shot and batch-to-batch variations for the four analytes were less than 10% and 15%, respectively). We validated the practicality of this approach-considering its advantages in sensitivity, repeatability, rapidity, and simplicity-through the analysis of testosterone in a urine sample.
authors: Department of Applied Science, National Taitung University, 684, Section 1, Chunghua Road, Taitung, 95002, Taiwan. tcchiu@nttu.edu.tw
authors: Tai-Chia
authors: TC
authors: Chiu
mesh_terms: D000328:Adult; D002392:Catechin / Q000737:chemistry*; D003348:Cortisone / Q000032:analysis*; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D012739:Gonadal Steroid Hormones; C009495:titanium dioxide; D002392:Catechin; D014025:Titanium; D003348:Cortisone; D006854:Hydrocortisone