title: Regulation of lipin1 by nutritional status, adiponectin, sex and pituitary function in rat white adipose tissue.
abstract: Lipin1 is a member of the lipin protein family that plays an important role in the regulation of lipid metabolism. The endogenous role of lipin1 was demonstrated by the fact that mutations in lipin1 caused lipodystrophy and metabolic disorders. The aim of this study was to assess the influence of nutritional status, pregnancy, insulin-sensitizers and pituitary hormones on lipin1 mRNA levels in adipose tissue of rats. Lipin1 gene expression was induced in conditions of hypoleptinemia (fasting) and leptin resistance (high fat diet), whereas it was decreased by high circulating leptin levels (leptin administration, pregnancy) and in leptin-deficient mice. Lipin1 mRNA levels were also decreased in adiponectin-deficient mice. Lipin1 mRNA levels are influenced by age in female rats, with peak expression at 25th day of life and decreasing thereafter. Consistently, ovariectomy increased lipin1 expression indicating that estrogens modulate lipin1. Finally, lipin1 was also regulated by pituitary hormones, since its expression was modified by thyroid status and growth hormone deficiency. Our observations indicate that: a) gWAT lipin1 mRNA levels are regulated by nutritional status, and leptin plays an important role in this regard, b) lipin1 is modulated by adiponectin, c) lipin1 is influenced by age and sex, and d) alterations in pituitary function modify lipin1 mRNA levels. To dissect the complicated interactions between key regulators of lipid metabolism like lipin1, may be important for the development of new therapies for the treatment and prevention of obesity and its associated disorders.
authors: Department of Physiology, School of Medicine-Instituto de Investigaciones Sanitarias (IDIS), University of Santiago de Compostela, Spain.
authors: C Ruth
authors: CR
authors: González
authors: Marta G
authors: MG
authors: Novelle
authors: Jorge E
authors: JE
authors: Caminos
authors: María J
authors: MJ
authors: Vázquez
authors: Raul M
authors: RM
authors: Luque
authors: Miguel
authors: M
authors: López
authors: Ruben
authors: R
authors: Nogueiras
authors: Carlos
authors: C
authors: Diéguez
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002369:Castration; D059305:Diet, High-Fat; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D013006:Growth Hormone / Q000172:deficiency; D020738:Leptin / Q000172:deficiency / Q000235:genetics; D008297:Male; D051379:Mice; D008820:Mice, Obese; D059645:Mutation Rate; D009752:Nutritional Status / Q000235:genetics / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D012991:Solanaceous Alkaloids / Q000235:genetics / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism
chemical_list: D052242:Adiponectin; D020738:Leptin; D012333:RNA, Messenger; D012991:Solanaceous Alkaloids; C410862:leptine I; D013006:Growth Hormone