title: Fluorescence measurement of mitochondrial membrane potential changes in cultured cells.
abstract: The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
authors: Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA 94945-1400, USA. dnicholls@buckinstitute.org
authors: David G
authors: DG
authors: Nicholls
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism; D005456:Fluorescent Dyes; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009942:Organometallic Compounds; D061251:Primary Cell Culture; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D020112:Rhodamine 123
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D016194:Receptors, N-Methyl-D-Aspartate; C110932:tetramethyl rhodamine ethyl ester; D020112:Rhodamine 123