title: Microfluidic 3D bone tissue model for high-throughput evaluation of wound-healing and infection-preventing biomaterials.
abstract: We report the use of a microfluidic 3D bone tissue model, as a high-throughput means of evaluating the efficacy of biomaterials aimed at accelerating orthopaedic implant-related wound-healing while preventing bacterial infection. As an example of such biomaterials, inkjet-printed micropatterns were prepared to contain antibiotic and biphasic calcium phosphate (BCP) nanoparticles dispersed in a poly(D,L-lactic-co-glycolic) acid matrix. The micropatterns were integrated with a microfluidic device consisting of eight culture chambers. The micropatterns immediately and completely killed Staphylococcus epidermidis upon inoculation, and enhanced the calcified extracellular matrix production of osteoblasts. Without antibiotic elution, bacteria rapidly proliferated to result in an acidic microenvironment which was detrimental to osteoblasts. These results were used to demonstrate the tissue model's potential in: (i) significantly reducing the number of biomaterial samples and culture experiments required to assess in vitro efficacy for wound-healing and infection prevention and (ii) in situ monitoring of dynamic interactions of biomaterials with bacteria as wells as with tissue cells simultaneously.
authors: Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
authors: Joung-Hyun
authors: JH
authors: Lee
authors: Yexin
authors: Y
authors: Gu
authors: Hongjun
authors: H
authors: Wang
authors: Woo Y
authors: WY
authors: Lee
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013203:Staphylococcal Infections / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000187:drug effects; D023822:Tissue Engineering / Q000295:instrumentation*; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C020243:calcium phosphate