title: Survey of protein engineering strategies.
abstract: Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, directed evolution, and combinations of them. Different strategies are presented for identifying the best mutagenesis method, how to identify desired variants by screening or selection, and examples for successful applications are given. This should enable researchers to choose the most promising tools to solve their protein engineering challenges.
authors: Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany.
authors: Uwe
authors: U
authors: Bornscheuer
authors: Department of Biochemistry, Molecular Biology and Biophysics and the Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota.
authors: Romas J
authors: RJ
authors: Kazlauskas
mesh_terms: D000818:Animals; D019020:Directed Molecular Evolution / Q000379:methods; D006801:Humans; D016296:Mutagenesis; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D011506:Proteins; D011994:Recombinant Proteins