title: Normal mouse intestinal epithelial cells as a model for the in vitro invasion of Trichinella spiralis infective larvae.
abstract: It has been known for many years that Trichinella spiralis initiates infection by penetrating the columnar epithelium of the small intestine; however, the mechanisms used by the parasite in the establishment of its intramulticellular niche in the intestine are unknown. Although the previous observations indicated that invasion also occurs in vitro when the infective larvae are inoculated onto cultures of intestinal epithelial cells (e.g., human colonic carcinoma cell line Caco-2, HCT-8), a normal readily manipulated in vitro model has not been established because of difficulties in the culture of primary intestinal epithelial cells (IECs). In this study, we described a normal intestinal epithelial model in which T. spiralis infective larvae were shown to invade the monolayers of normal mouse IECs in vitro. The IECs derived from intestinal crypts of fetal mouse small intestine had the ability to proliferate continuously and express specific cytokeratins as well as intestinal functional cell markers. Furthermore, they were susceptible to invasion by T. spiralis. When inoculated onto the IEC monolayer, infective larvae penetrated cells and migrated through them, leaving trails of damaged cells heavily loaded with T. spiralis larval excretory-secretory (ES) antigens which were recognized by rabbit immune sera on immunofluorescence test. The normal intestinal epithelial model of invasion mimicking the natural environment in vivo will help us to further investigate the process as well as the mechanisms by which T. spiralis establishes its intestinal niche.
authors: Department of Parasitology, Medical College, Zhengzhou University, PR China.
authors: Hui Jun
authors: HJ
authors: Ren
authors: Jing
authors: J
authors: Cui
authors: Zhong Quan
authors: ZQ
authors: Wang
authors: Ruo Dan
authors: RD
authors: Liu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000469:parasitology* / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007422:Intestines / Q000473:pathology*; D007814:Larva / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008858:Microscopy, Phase-Contrast; D008954:Models, Biological; D011817:Rabbits; D013194:Staining and Labeling; D017160:Trichinella spiralis / Q000502:physiology*; D014235:Trichinellosis / Q000469:parasitology* / Q000473:pathology*