title: Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin.
abstract: Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrin-independent endocytic pathways have been described in other eukaryotes. Here, we provide evidence for a clathrin-independent endocytic pathway in yeast. In cells lacking the clathrin-binding adaptor proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2. This second pathway does not require components of the better-studied endocytic pathway, including clathrin and Arp2/3 complex activators. Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells.
authors: Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.
authors: Derek C
authors: DC
authors: Prosser
authors: Theodore G
authors: TG
authors: Drivas
authors: Lymarie
authors: L
authors: Maldonado-Báez
authors: Beverly
authors: B
authors: Wendland
mesh_terms: D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D008840:Microfilament Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
chemical_list: C105191:Bni1 protein, S cerevisiae; D002966:Clathrin; D008840:Microfilament Proteins; D029701:Saccharomyces cerevisiae Proteins; C051302:RHO1 protein, S cerevisiae; D020741:rho GTP-Binding Proteins