title: RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing.
abstract: The RNA polymerase II (RNAP II) largest subunit contains a C-terminal domain (CTD) with up to 52 Tyr(1)-Ser(2)-Pro(3)-Thr(4)-Ser(5)-Pro(6)-Ser(7) consensus repeats. Serines 2, 5, and 7 are known to be phosphorylated, and these modifications help to orchestrate the interplay between transcription and processing of messenger RNA (mRNA) precursors. Here, we provide evidence that phosphorylation of CTD Thr(4) residues is required specifically for histone mRNA 3' end processing, functioning to facilitate recruitment of 3' processing factors to histone genes. Like Ser(2), Thr(4) phosphorylation requires the CTD kinase CDK9 and is evolutionarily conserved from yeast to human. Our data thus illustrate how a CTD modification can play a highly specific role in facilitating efficient gene expression.
authors: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors: Jing-Ping
authors: JP
authors: Hsin
authors: Amit
authors: A
authors: Sheth
authors: James L
authors: JL
authors: Manley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002645:Chickens; D039223:Cleavage And Polyadenylation Specificity Factor / Q000378:metabolism; D042863:Cyclin-Dependent Kinase 9 / Q000378:metabolism; D006657:Histones / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D026721:RNA 3' End Processing; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013912:Threonine / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000378:metabolism
chemical_list: D039223:Cleavage And Polyadenylation Specificity Factor; D006657:Histones; D009687:Nuclear Proteins; D012333:RNA, Messenger; C092663:SLBP protein, human; D039221:mRNA Cleavage and Polyadenylation Factors; D013912:Threonine; C467969:CDK9 protein, human; D042863:Cyclin-Dependent Kinase 9; D012319:RNA Polymerase II; C410949:RNA polymerase II largest subunit