title: [Antiproliferative effects mechanism of beta-sitosterul in hepatoma HepG2 cells].
abstract: OBJECTIVE
  To study the antiproliferative effects of beta-sitosterul and its mechanism in hepatoma HepG2 cells.
  
  
  METHODS
  Cell proliferation was assessed by MTT assay. Cell cycle distribution, apoptosis and mitochondrial membrane potential were measured by high content screening (HCS). The protein expression of caspase-3, caspase-8, caspase-9, Bcl-2, Bax, tBid and cytochrome c in the HepG2 cells were evaluated by Western Blots.
  
  
  RESULTS
  beta-Sitosterul exerted significant antiproliferative effects in HepG2 cells. Furthermore, beta-sitosterul also induced HepG2 cells apoptosis, lost mitochondrial membrane potential, activated caspase-3, caspase-8 and caspase-9, up-regulate Bax, tBid protein, down-regulation Bcl-2 protein. However, beta-sitosterul had hardly any effects on QSG7701 cells.
  
  
  CONCLUSIONS
  beta-Sitosterul exerted antiproliferative effects and induced HepG2 cells apoptosis via mitochondrial pathway and membrane death receptor pathway.
authors: Institute of Chemistry & Biology, Henan University, Kaifeng 475004, China.
authors: Zhongquan
authors: Z
authors: Zhang
authors: Yujun
authors: Y
authors: Xing
authors: Guoqiang
authors: G
authors: Hu
authors: Songqiang
authors: S
authors: Xie
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012855:Sitosterols / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D012855:Sitosterols; C025473:gamma-sitosterol