title: The size and phospholipid composition of lipid droplets can influence their proteome.
abstract: The proteomic makeup of lipid droplets (LDs) is believed to regulate the function of LDs, which are now recognized as important cellular organelles that are associated with many human metabolic disorders. However, factors that help determine LD proteome remain to be identified and characterized. Here we analyzed the phospholipid and protein composition of LDs isolated from wild type (WT) yeast cells, and also from fld1Δ, cds1, and ino2Δ mutant cells which produce 'supersized' LDs. LDs of fld1Δ and WT cells exhibited similar phospholipid profiles, whereas LDs of cds1 and ino2Δ strains had a higher (cds1) or lower (ino2Δ) percentage of phosphatidylcholine than those of WT, respectively. Unexpectedly, the presence of most known LD resident proteins was greatly reduced in the LD fraction isolated from cds1 and ino2Δ, including neutral lipid hydrolases. Consistent with this result, mobilization of neutral lipids was seriously impaired in these two strains. Contrary to the reduction of LD resident proteins, the Hsp90 family molecular chaperones, Hsc82 and Hsp82, were greatly increased in the LD fractions of cds1 and ino2Δ strains without changes at the level of expression. These data demonstrate the impact of LD phopholipids and size on the makeup of LD proteome.
authors: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052, Australia.
authors: Weihua
authors: W
authors: Fei
authors: Ling
authors: L
authors: Zhong
authors: Minh Truong
authors: MT
authors: Ta
authors: Guanghou
authors: G
authors: Shui
authors: Markus R
authors: MR
authors: Wenk
authors: Hongyuan
authors: H
authors: Yang
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry*; D020543:Proteome; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D008565:Membrane Proteins; D009713:Nucleotidyltransferases; D010743:Phospholipids; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; C527368:Fld1 protein, S cerevisiae; C095723:INO2 protein, S cerevisiae; C022348:phosphatidate cytidylyltransferase