title: Isolation, characterization and mobilization of prostate cancer tissue derived CD133+ MDR1+ cells.
abstract: Cancer stem cells undoubtedly exist in many tumor types, including the prostate. CD133 has recently been considered an important marker that represents the subset population of cancer stem cells. The purpose of the present study is to isolate CD133+ and CD133- cells from normal healthy volunteers and prostate cancer patients to check the prostate stem cells markers and chemokine receptors like CXCR4 for their mobilization. In this study we isolated CD133+ and CD133- cells using magnetic beads from prostate tissues and peripheral blood samples of normal healthy volunteers and prostate cancer patients (NV-CD133+, NV-CD133-, PC-CD133+ and PC-CD133-). The isolated cells were analyzed using flow cytometry and western blot analysis of MDR1, alpha2beta1, CD44, Oct-4 and CXCR4. PC-CD133+ cells displayed higher expressions of CD44, MDR1, Oct-4 and alpha2beta1 expressions with the ability to differentiate into prostate cancer cells. Furthermore, PC-CD133+, highly expressed the chemokine receptor CXCR4 and its ligand SDF1-alpha. Here we conclude that, PC-CD133+ displayed a higher expression of prostate stem cell markers like CD44, MDR1 and Oct-4 and chemokine receptor CXCR4 and its ligand SDF1-alpha in peripheral blood and prostate cancer tissue derived CD133+ cells The CXCR4 and SDF1-alpha expressions will have impact on the mobilization of prostate cancer stem cells (PC-CD133+ cells).
authors: Department of Biotechnology (Engg), GITAM Institute of Technology, GITAM University, Rushikonda, Visakhapatnam, India. s.rentala@yahoo.com
authors: Satyanarayana
authors: S
authors: Rentala
authors: Lakshmi Narasu
authors: LN
authors: Mangamoori
mesh_terms: D000071916:AC133 Antigen; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D010455:Peptides / Q000378:metabolism*; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D019718:Receptors, CXCR4 / Q000378:metabolism*
chemical_list: C513055:ABCB1 protein, human; D000071916:AC133 Antigen; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D019718:Receptors, CXCR4