title: Role of neutrophils in acrylonitrile-induced gastric mucosal damage.
abstract: Acrylonitrile (ACN) is a widely used intermediate in the manufacture of plastics, acrylic ﬁbers, synthetic rubbers and resins that are used in a variety of products including food containers and medical devices. ACN is a possible human carcinogen and a documented animal carcinogen, with the stomach being an important target of its toxicity. ACN has been previously reported to require metabolic activation to reactive intermediates and finally to cyanide (CN⁻). The current study aimed at exploring the potential role of neutrophils in ACN-induced gastric damage in rats. Experimental neutropenia was attained by injecting rats with methotrexate. This significantly ameliorated gastric mucosal injury induced by ACN. This is evidenced by protection against the increase in gastric ulcer index, myeloperoxidase (MPO) activity and CN⁻ level. Also, neutropenia guarded against the decrease in prostaglandin E2 (PGE2), induction of oxidative stress and reduction of total nitrites and alleviated histopathological alterations in rat stomachs. These data indicate that neutrophil infiltration is, at least partly, involved in ACN-induced gastric damage in rats.
authors: Department of Biochemistry, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors: Nadia M
authors: NM
authors: Hamdy
authors: Fahad A
authors: FA
authors: Al-Abbasi
authors: Hassan A
authors: HA
authors: Alghamdi
authors: Mai F
authors: MF
authors: Tolba
authors: Ahmed
authors: A
authors: Esmat
authors: Ashraf B
authors: AB
authors: Abdel-Naim
mesh_terms: D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D002374:Catalase / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D005978:Glutathione / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D009195:Peroxidase / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D000181:Acrylonitrile