title: Wobble base-pairing slows in vivo translation elongation in metazoans.
abstract: In the universal genetic code, most amino acids can be encoded by multiple trinucleotide codons, and the choice among available codons can influence position-specific translation elongation rates. By using sequence-based ribosome profiling, we obtained transcriptome-wide profiles of in vivo ribosome occupancy as a function of codon identity in Caenorhabditis elegans and human cells. Particularly striking in these profiles was a universal trend of higher ribosome occupancy for codons translated via G:U wobble base-pairing compared with synonymous codons that pair with the same tRNA family using G:C base-pairing. These data support a model in which ribosomal translocation is slowed at wobble codon positions.
authors: Department of Genetics, Stanford University, Stanford, California 94305-5324, USA.
authors: Michael
authors: M
authors: Stadler
authors: Andrew
authors: A
authors: Fire
mesh_terms: D000818:Animals; D000926:Anticodon / Q000737:chemistry*; D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D003062:Codon / Q000737:chemistry*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D010441:Peptide Chain Elongation, Translational / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D000926:Anticodon; D003062:Codon; D012333:RNA, Messenger; D012343:RNA, Transfer