title: [TSA improve transgenic porcine cloned embryo development and transgene expression].
abstract: Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. TSA prevents the chromatin structure from being condensed, so that transcription factor could binds to DNA sequence easily and enhance transgene expression. Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression.
authors: College of Life Science, Northeast Agricultural University of China, Harbin 150030, China. kqr1982@yahoo.com.cn
authors: Qing-Ran
authors: QR
authors: Kong
authors: Jiang
authors: J
authors: Zhu
authors: Bo
authors: B
authors: Huang
authors: Yan-Jun
authors: YJ
authors: Huan
authors: Feng
authors: F
authors: Wang
authors: Yong-Qian
authors: YQ
authors: Shi
authors: Zhong-Feng
authors: ZF
authors: Liu
authors: Mei-Ling
authors: ML
authors: Wu
authors: Zhong-Hua
authors: ZH
authors: Liu
mesh_terms: D000107:Acetylation; D000818:Animals; D002999:Clone Cells; D019976:Cloning, Organism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D013552:Swine / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes / Q000187:drug effects*
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A