title: Effect on the production of soluble endoglin from human choriocarcinoma cells by preeclampsia sera.
abstract: OBJECTIVE
  The soluble endoglin (sEng) is an antiangiogenic protein that may inhibit TGF-β1 signaling and endothelial nitric oxide synthase activation in endothelial cells. The levels of sEng increased in sera obtained from preeclampsia. The factors that increase the sEng in preeclampsia have not been known well. To investigate the factors that may increase sEng in preeclampsia, we examined the effect of preeclampsia sera on the production of sEng from human choriocarcinoma (JEG-3) cells.
  
  
  METHODS
  Serum samples were taken from women with normal pregnancy and from those with preeclampsia. JEG-3 cells were cultured with serum for 24 hrs, and the sEng levels in supernatants and expression of sEng and Hemo oxygenase-1 (HO-1) mRNA in cells were measured.
  
  
  RESULTS
  The addition of preeclampsia sera into JEG-3 cells led to increased release of sEng and expression of Eng mRNA. Preeclampsia sera inhibited the expression of HO-1 mRNA in JEG-3 cells.
  
  
  CONCLUSIONS
  The results suggest that preeclampsia sera may increase the protein production of sEng and mRNA expression of Eng from JEG-3 cells like trophoblast without hypoxia and that in addition to hypoxia, preeclampsia sera may play a role of high level of serum sEng in preeclampsia patients. Decreased HO-1 activity may relate to increased sEng release.
authors: Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan.
authors: Youichi
authors: Y
authors: Aoki
authors: Tatsuo
authors: T
authors: Yamamoto
authors: Chishima
authors: C
authors: Fumihisa
authors: Akikazu
authors: A
authors: Nakamura
authors: Aki
authors: A
authors: Asanuma
authors: Manami
authors: M
authors: Suzuki
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000378:metabolism*; D000071063:Endoglin; D005260:Female; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D051547:Heme Oxygenase-1