title: Phlebotomine vector ecology in the domestic transmission of American cutaneous leishmaniasis in Chaparral, Colombia.
abstract: Phlebotomine vector ecology was studied in the largest recorded outbreak of American cutaneous leishmaniasis in Colombia in 2004. In two rural townships that had experienced contrasting patterns of case incidence, this study evaluated phlebotomine species composition, seasonal abundance, nocturnal activity, blood source, prevalence of Leishmania infection, and species identification. CDC miniature light traps were used to trap the phlebotomines. Traps were set indoors, peridomestically, and in woodlands. Natural infection was determined in pools by polymerase chain reaction-Southern blot, and blood sources and species identification were determined by sequencing. Large differences were observed in population abundance between the two townships evaluated. Lutzomyia longiflocosa was the most abundant species (83.1%). Abundance was higher during months with lower precipitation. Nocturnal activity was associated with human domestic activity. Blood sources identified were mainly human (85%). A high prevalence of infection was found in L. longiflocosa indoors (2.7%) and the peridomestic setting (2.5%). L. longiflocosa was responsible for domestic transmission in Chaparral.
authors: Laboratorio de Entomología, Instituto Nacional de Salud, Bogotá, Colombia. crisferrovela@gmail.com
authors: Cristina
authors: C
authors: Ferro
authors: Dairo
authors: D
authors: Marín
authors: Rafael
authors: R
authors: Góngora
authors: María C
authors: MC
authors: Carrasquilla
authors: Jorge E
authors: JE
authors: Trujillo
authors: Norma K
authors: NK
authors: Rueda
authors: Jaime
authors: J
authors: Marín
authors: Carlos
authors: C
authors: Valderrama-Ardila
authors: Neal
authors: N
authors: Alexander
authors: Mauricio
authors: M
authors: Pérez
authors: Leonard E
authors: LE
authors: Munstermann
authors: Clara B
authors: CB
authors: Ocampo
mesh_terms: D000818:Animals; D001483:Base Sequence; D003105:Colombia / Q000453:epidemiology; D004196:Disease Outbreaks; D017753:Ecosystem; D005191:Family Characteristics; D005260:Female; D006801:Humans; D015994:Incidence; D007303:Insect Vectors / Q000469:parasitology / Q000502:physiology*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000635:transmission*; D011157:Population Dynamics; D011576:Psychodidae / Q000145:classification / Q000235:genetics / Q000469:parasitology / Q000502:physiology*; D011891:Rain; D012621:Seasons; D013045:Species Specificity; D013997:Time Factors