title: Biological responses to diesel exhaust particles (DEPs) depend on the physicochemical properties of the DEPs.
abstract: Diesel exhaust particles (DEPs) are the main components of ambient particulate materials, including polyaromatic hydrocarbons (PAHs), n-PAHs, heavy metals, and gaseous materials. Many epidemiological, clinical, and toxicological studies have shown that ambient particles, including DEPs, are associated with respiratory disorders, such as asthma, allergic rhinitis, and lung cancer. However, the relationship between the biological response to DEPs and their chemical composition remains unclear. In this study, we investigated the physicochemical properties of DEPs before toxicological studies, and then administered a single intratracheal instillation of DEPs to mice. The mice were then killed 1, 7, 14 and 28 days after DEP exposure to observe the biological responses induced by DEPs over time. Our findings suggest that DEPs engulfed into cells induced a Th2-type inflammatory response followed by DNA damage, whereas DEPs not engulfed into cells induced a Th1-type inflammatory response. Further, the physicochemical properties, including surface charge, particle size, and chemical composition, of DEPs play a crucial role in determining the biological responses to DEPs. Consequently, we suggest that the biological response to DEPs depend on cell-particle interaction and the physicochemical properties of the particles.
authors: Department of Molecular Science and Technology, Ajou University, Suwon, Korea.
authors: Eun-Jung
authors: EJ
authors: Park
authors: Jinkyu
authors: J
authors: Roh
authors: Min-Sung
authors: MS
authors: Kang
authors: Soo Nam
authors: SN
authors: Kim
authors: Younghun
authors: Y
authors: Kim
authors: Sangdun
authors: S
authors: Choi
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004249:DNA Damage; D016130:Immunophenotyping; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010587:Phagocytosis; D001335:Vehicle Emissions
chemical_list: D001335:Vehicle Emissions