title: Synthesis of genetically engineered protein polymers (recombinamers) as an example of advanced self-assembled smart materials.
abstract: In this chapter, we describe two methods for bio-producing recombinant repetitive polypeptide polymers for use in biomedical devices. These polymers, known as elastin-like recombinamers (ELRs), are derived from the repetition of selected amino acid domains of extracellular matrix proteins with the aim of recreating their mechanical and physiological features. The proteinaceous nature of ELRs allows us to make use of the natural biosynthetic machinery of heterologous hosts to express advanced and large polymers or "recombinamers." Despite the essentially unlimited possibilities for designing recombinamers, the production of synthetic genes to encode them should allow us to overcome the difficulties surrounding bioproduction of these non-natural monotonous DNA and protein sequences. The aim of this work is to supply the biotechnologist with fine-tuning methods to biosynthesize advanced self-assembled smart materials.
authors: G.I.R. Bioforge, University of Valladolid, CIBER-BBN, Valladolid, Spain. cabello@bioforge.uva.es
authors: José Carlos
authors: JC
authors: Rodríguez-Cabello
authors: Alessandra
authors: A
authors: Girotti
authors: Artur
authors: A
authors: Ribeiro
authors: Francisco Javier
authors: FJ
authors: Arias
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004549:Elastin / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D010452:Peptide Biosynthesis; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D010455:Peptides; D011994:Recombinant Proteins; D004549:Elastin