title: Multifunctional nanoprobes for upconversion fluorescence, MR and CT trimodal imaging.
abstract: Early diagnosis probes that combine fluorescence, X-ray computed tomography (CT) and magnetic resonance (MR) imagings are anticipated to give three dimensional (3D) details of tissues and cells of high resolution and sensitivity. However, how to combine these three modalities together within a sub-50 nm sized structure is technically challenging. Here we report a trimodal imaging probe of PEGylated NaY/GdF(4): Yb, Er, Tm @SiO(2)-Au@PEG(5000) nanopaticles of uniform size of less than 50 nm. The as-designed nanoprobes showed (1) strong emissions ranging from the visible (Vis) to near infrared (NIR) for fluorescent imaging, (2) T(1)-weighted MRI by shorting T(1) relaxation time and (3) enhanced HU value as a CT contrast agent. The structure was optimized based on a comprehensive investigation on the influence of the distance between the NaY/GdF(4): Yb, Er, Tm core and Au nanoparticles (NPs) at the surface. The potential of trimodal imaging for cancerous cells and lesions was further demonstrated both in vitro and in vivo.
authors: State Key Laboratory of High Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors: Huaiyong
authors: H
authors: Xing
authors: Wenbo
authors: W
authors: Bu
authors: Shengjian
authors: S
authors: Zhang
authors: Xiangpeng
authors: X
authors: Zheng
authors: Ming
authors: M
authors: Li
authors: Feng
authors: F
authors: Chen
authors: Qianjun
authors: Q
authors: He
authors: Liangping
authors: L
authors: Zhou
authors: Weijun
authors: W
authors: Peng
authors: Yanqing
authors: Y
authors: Hua
authors: Jianlin
authors: J
authors: Shi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005453:Fluorescence; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis*; D014057:Tomography, X-Ray Computed / Q000379:methods*