title: Elucidation of structure-function relationships in plant major light-harvesting complex (LHC II) by nonlinear spectroscopy.
abstract: Conventional linear and time-resolved spectroscopic techniques are often not appropriate to elucidate specific pigment-pigment interactions in light-harvesting pigment-protein complexes (LHCs). Nonlinear (laser-) spectroscopic techniques, including nonlinear polarization spectroscopy in the frequency domain (NLPF) as well as step-wise (resonant) and simultaneous (non-resonant) two-photon excitation spectroscopies may be advantageous in this regard. Nonlinear spectroscopies have been used to elucidate substructure(s) of very complex spectra, including analyses of strong excitonic couplings between chlorophylls and of interactions between (bacterio)chlorophylls and "optically dark" states of carotenoids in LHCs, including the major antenna complex of higher plants, LHC II. This article shortly reviews our previous study and outlines perspectives regarding the application of selected nonlinear laser-spectroscopic techniques to disentangle structure-function relationships in LHCs and other pigment-protein complexes.
authors: Institut für Biochemie und Biologie/Pflanzenphysiologie, Universität Potsdam, Karl-Liebknecht-Str. 24-25, Haus 20, 14476, Potsdam-Golm, Germany. lokstein@uni-potsdam.de
authors: Heiko
authors: H
authors: Lokstein
authors: Alexander
authors: A
authors: Betke
authors: Maria
authors: M
authors: Krikunova
authors: Klaus
authors: K
authors: Teuchner
authors: Bernd
authors: B
authors: Voigt
mesh_terms: D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000378:metabolism*; D017711:Nonlinear Dynamics; D010788:Photosynthesis; D010860:Pigments, Biological / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D045342:Light-Harvesting Protein Complexes; D010860:Pigments, Biological