title: [Comparative study of three primary culture methods of human epithelial cells of nasal polyps in vitro].
abstract: OBJECTIVE
  To compare three primary culture methods of human epithelial cells of nasal polyps in vitro, and explore a suitable method to be used in further study.
  
  
  METHODS
  Shape and appearance of human epithelial cells of nasal polyps were observed, and success ratio and growth curve of primarily cultural were achieved by enzymatic dissociation, isolated cell ABC culture and tissue piece culture method respectively.
  
  
  RESULTS
  By comparing achievement ratios, the isolated cell ABC culture method (87.5%) was shown to be superior to enzymatic dissociation (78.13%) and tissue piece culture methods (83.33%), but no statistical significance was found (P>0.05). The growth curves of two isolated cell culture methods were higher than that of tissue piece culture.
  
  
  CONCLUSIONS
  The isolated cell ABC culture model is more suitable for primary culture due to its faster proliferation, less promiscuity, and more stable and reliable cell supply for nasal polyps research.
authors: Department of Otolaryngology-Head and Neck Surgery, the Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China.
authors: Mingming
authors: M
authors: Wang
authors: Guodong
authors: G
authors: Zhang
authors: Xiaoting
authors: X
authors: Wang
authors: Jianfeng
authors: J
authors: Li
authors: Haibo
authors: H
authors: Wang
mesh_terms: D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology*; D009298:Nasal Polyps