title: Absence of Helicobacter pylori in healthy laryngeal mucosa.
abstract: OBJECTIVE
  To evaluate the presence of Helicobacter pylori in healthy laryngeal mucosa.
  
  
  METHODS
  Prospective analysis of Helicobacter pylori colonisation in healthy laryngeal mucosa, using the 13C urea breath test and polymerase chain reaction analysis.
  
  
  METHODS
  Twenty randomly chosen men (28-78 years) without laryngeal pathology or gastrointestinal disease were investigated. All subjects were scheduled for elective operative procedures, under general, endotracheal anaesthesia. Cytobrush samples were taken for Helicobacter pylori DNA detection. Nested polymerase chain reaction testing was performed on DNA solutions using two primer pairs from the urease A gene of the Helicobacter pylori genome. The 13C urea breath test was performed on two exhalation samples.
  
  
  RESULTS
  Eight (40 per cent) of the subjects were positive for urease on urea breath testing; none were positive for Helicobacter pylori DNA on polymerase chain reaction testing.
  
  
  CONCLUSIONS
  Based on these results, we do not consider Helicobacter pylori to be a normal constituent of healthy laryngeal microflora.
authors: ENT Department, Dr Josip Benčević General Hospital, Slavonski Brod, Croatia. ivana.pajic-penavic@sb.t-com.hr
authors: I
authors: I
authors: Pajić-Penavić
authors: D
authors: D
authors: Danić
authors: S
authors: S
authors: Maslovara
authors: K
authors: K
authors: Gall-Trošelj
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests / Q000379:methods; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000453:epidemiology; D016480:Helicobacter pylori / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D007820:Laryngeal Mucosa / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014508:Urea / Q000378:metabolism*; D014510:Urease / Q000032:analysis
chemical_list: D002247:Carbon Isotopes; D004269:DNA, Bacterial; D014508:Urea; D014510:Urease