title: Carbohydrate microarrays in 96-well polystyrene microtiter plates.
abstract: A method for the preparation of carbohydrate microarrays inside 96-well polystyrene microtiter plates is described. The key step in this strategy represents the synthesis of carbohydrate-dextran conjugates by copper (I)-catalyzed [3 + 2] cycloaddition between alkyne carbohydrate derivative and a specially designed azido dextran polymer. The conjugates adsorb efficiently on polystyrene surface and can be printed inside 96-well plates using a non-contact piezoelectric microarrayer. Model interactions with a selection of lectins (concanavalin A, wheat germ agglutinin, Erythrina Cristagalli) display the efficiency of the immobilization method, its reproducibility and the specificity of biomolecular interactions occurring at the polystyrene-water interface.
authors: CNRS UMR 8161, Université Lille Nord de France, Institut Pasteur de Lille, IFR 142 Molecular and Cellular Medicine, Lille, France.
authors: Jean-Philippe
authors: JP
authors: Ebran
authors: Nabil
authors: N
authors: Dendane
authors: Oleg
authors: O
authors: Melnyk
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D003911:Dextrans; D011137:Polystyrenes