title: Inhibitory activities of pancreatic lipase and phosphodiesterase from Korean medicinal plant extracts.
abstract: To find new pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) inhibitors from natural products, 61 medicinal plants from Korea were screened for their antilipase activity for prevention of obesity. Dried and powdered plants were extracted three times with EtOH and extracts were obtained by removal of the solvent in vacuo. Lipase activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol. Also, the inhibitory effect was measured on phosphodiesterase (PDE), another therapeutic target for obesity. Of the extracts tested, Sorbus commixta (stem, leaf) and Viscum album (whole plant) exhibited antilipase activity (with IC(50) values of 29.6 µg/mL and 33.3 µg/mL, respectively) and only anti-PDE activity (IC(50) values of 20.08 µg/mL and 35.15 µg/mL, respectively).
authors: Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Daejeon, 305-811, Republic of Korea.
authors: Yun Mi
authors: YM
authors: Lee
authors: Young Sook
authors: YS
authors: Kim
authors: Youngseop
authors: Y
authors: Lee
authors: Junghyun
authors: J
authors: Kim
authors: Hang
authors: H
authors: Sun
authors: Joo Hwan
authors: JH
authors: Kim
authors: Jin Sook
authors: JS
authors: Kim
mesh_terms: D006801:Humans; D020128:Inhibitory Concentration 50; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009765:Obesity / Q000517:prevention & control; D010179:Pancreas / Q000201:enzymology; D010726:Phosphodiesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D056910:Republic of Korea; D031994:Sorbus / Q000737:chemistry*; D028182:Viscum album / Q000737:chemistry*
chemical_list: D010726:Phosphodiesterase Inhibitors; D010936:Plant Extracts; D008049:Lipase; D010727:Phosphoric Diester Hydrolases