title: The quantitative proteome of a human cell line.
abstract: The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least -10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.
authors: European Molecular Biology Laboratory, Heidelberg, Germany.
authors: Martin
authors: M
authors: Beck
authors: Alexander
authors: A
authors: Schmidt
authors: Johan
authors: J
authors: Malmstroem
authors: Manfred
authors: M
authors: Claassen
authors: Alessandro
authors: A
authors: Ori
authors: Anna
authors: A
authors: Szymborska
authors: Franz
authors: F
authors: Herzog
authors: Oliver
authors: O
authors: Rinner
authors: Jan
authors: J
authors: Ellenberg
authors: Ruedi
authors: R
authors: Aebersold
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D013058:Mass Spectrometry; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D049490:Systems Biology / Q000379:methods
chemical_list: D020543:Proteome