title: Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth.
abstract: Surfaces in industrial settings provide a home for resident biofilms that are likely to interact with the attachment, growth and survival of pathogens such as Listeria monocytogenes. Experimental results have indicated that L. monocytogenes cells were inhibited by the presence of a model resident flora (Lactococcus lactis) in dual-species continuous flow-biofilms, and are spatially restricted to the lower biofilm layers. Using a new, simplified individual-based model (IBM) that simulates bacterial cell growth in a three-dimensional space, the spatial arrangements of the two species were reconstructed and their cell counts successfully predicted. This model showed that the difference in generation times between L. monocytogenes and L. lactis cells during the initial stages of dual-species biofilm formation was probably responsible for the species spatialization observed and the subsequent inhibition of growth of the pathogen.
authors: INRA, UMR 1319 MICALIS, F-78350, Jouy-en-Josas, France.
authors: Olivier
authors: O
authors: Habimana
authors: Laurent
authors: L
authors: Guillier
authors: Saulius
authors: S
authors: Kulakauskas
authors: Romain
authors: R
authors: Briandet
mesh_terms: D000898:Antibiosis; D058491:Bacterial Load; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media; D006801:Humans; D013294:Lactococcus lactis / Q000254:growth & development* / Q000502:physiology; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000502:physiology; D018613:Microscopy, Confocal
chemical_list: D003470:Culture Media