title: Anti-IL-33 antibody has a therapeutic effect in a murine model of allergic rhinitis.
abstract: BACKGROUND
  Interleukin (IL)-33 is involved in the Th2 immune response and could play an essential role in nasal allergy. Therefore, we aimed to investigate the therapeutic potential of anti-IL-33 for allergic rhinitis (AR).
  
  
  METHODS
  Twenty-four BALB/c mice were used. In group A (control group, n = 6), mice were sensitized and challenged with saline. Group B [ovalbumin (OVA) group, n = 6] mice received intraperitoneal and intranasal OVA challenge. In group C (control IgG group, n = 6), mice were injected intraperitoneally with rabbit control IgG before OVA challenge. In group D (anti-IL-33 group, n = 6), anti-IL-33 was injected before challenge. We evaluated the number of nose-scratching events and external morphology; serum total and OVA-specific IgE; number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathologic examination of nasal cavity; and IL-4, IL-5, and IL-13 in BAL fluid.
  
  
  RESULTS
  Anti-IL-33 treatment significantly reduced the nose-scratching events and ameliorated skin denudation. Serum total and OVA-specific IgE was significantly decreased in group D. The number of eosinophils in BAL fluid was also significantly decreased. Eosinophilic infiltration in the nasal cavity was significantly decreased in group D. IL-4, IL-5, and IL-13 in BAL fluid were also significantly decreased after treatment.
  
  
  CONCLUSIONS
  Anti-IL-33 antibody has a therapeutic potential for experimental AR.
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Inha University College of Medicine, Incheon, Korea.
authors: Y H
authors: YH
authors: Kim
authors: T Y
authors: TY
authors: Yang
authors: C-S
authors: CS
authors: Park
authors: S-H
authors: SH
authors: Ahn
authors: B K
authors: BK
authors: Son
authors: J H
authors: JH
authors: Kim
authors: D H
authors: DH
authors: Lim
authors: T Y
authors: TY
authors: Jang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology* / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007274:Injections, Intraperitoneal; D018793:Interleukin-13 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology
chemical_list: D000906:Antibodies; D016207:Cytokines; D018793:Interleukin-13; D007073:Immunoglobulin E; D010047:Ovalbumin