title: Standardization of preanalytical aspects of cerebrospinal fluid biomarker testing for Alzheimer's disease diagnosis: a consensus paper from the Alzheimer's Biomarkers Standardization Initiative.
abstract: BACKGROUND
  Numerous studies show that the cerebrospinal fluid biomarkers total tau (T-tau), tau phosphorylated at threonine 181 (P-tau(181P)), and amyloid-β (1-42) (Aβ(1-42)) have high diagnostic accuracy for Alzheimer's disease. Variability in concentrations for Aβ(1-42), T-tau, and P-tau(181P) drives the need for standardization.
  
  
  METHODS
  Key issues were identified and discussed before the first meeting of the members of the Alzheimer's Biomarkers Standardization Initiative (ABSI). Subsequent ABSI consensus meetings focused on preanalytical issues.
  
  
  RESULTS
  Consensus was reached on preanalytical issues such as the effects of fasting, different tube types, centrifugation, time and temperature before storage, storage temperature, repeated freeze/thaw cycles, and length of storage on concentrations of Aβ(1-42), T-tau, and P-tau(181P) in cerebrospinal fluid.
  
  
  CONCLUSIONS
  The consensus reached on preanalytical issues and the recommendations put forward during the ABSI consensus meetings are presented in this paper.
authors: Innogenetics NV, Ghent, Belgium.
authors: Hugo
authors: H
authors: Vanderstichele
authors: Mirko
authors: M
authors: Bibl
authors: Sebastiaan
authors: S
authors: Engelborghs
authors: Nathalie
authors: N
authors: Le Bastard
authors: Piotr
authors: P
authors: Lewczuk
authors: Jose Luis
authors: JL
authors: Molinuevo
authors: Lucilla
authors: L
authors: Parnetti
authors: Armand
authors: A
authors: Perret-Liaudet
authors: Leslie M
authors: LM
authors: Shaw
authors: Charlotte
authors: C
authors: Teunissen
authors: Dirk
authors: D
authors: Wouters
authors: Kaj
authors: K
authors: Blennow
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D017408:Guidelines as Topic / Q000592:standards*; D006801:Humans; D010446:Peptide Fragments / Q000134:cerebrospinal fluid; D011786:Quality Control; D015203:Reproducibility of Results; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D016875:tau Proteins