title: Cholesteryl butyrate solid lipid nanoparticles inhibit the adhesion and migration of colon cancer cells.
abstract: BACKGROUND AND PURPOSE Cholesteryl butyrate solid lipid nanoparticles (cholbut SLN) provide a delivery system for the anti-cancer drug butyrate. These SLN inhibit the adhesion of polymorphonuclear cells to the endothelium and may act as anti-inflammatory agents. As cancer cell adhesion to endothelium is crucial for metastasis dissemination, here we have evaluated the effect of cholbut SLN on adhesion and migration of cancer cells. EXPERIMENTAL APPROACH Cholbut SLN was incubated with a number of cancer cell lines or human umbilical vein endothelial cells (HUVEC) and adhesion was quantified by a computerized micro-imaging system. Migration was detected by the scratch 'wound-healing' assay and the Boyden chamber invasion assay. Expression of ERK and p38 MAPK was analysed by Western blot. Expression of the mRNA for E-cadherin and claudin-1 was measured by RT-PCR. KEY RESULTS Cholbut SLN inhibited HUVEC adhesiveness to cancer cell lines derived from human colon-rectum, breast, prostate cancers and melanoma. The effect was concentration and time-dependent and exerted on both cancer cells and HUVEC. Moreover, these SLN inhibited migration of cancer cells and substantially down-modulated ERK and p38 phosphorylation. The anti-adhesive effect was additive to that induced by the triggering of B7h, which is another stimulus inhibiting both ERK and p38 phosphorylation, and cell adhesiveness. Furthermore, cholbut SLN induced E-cadherin and inhibited claudin-1 expression in HUVEC. CONCLUSION AND IMPLICATIONS These results suggest that cholbut SLN could act as an anti-metastastic agent and they add a new mechanism to the anti-tumour activity of this multifaceted preparation of butyrate.
authors: Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Torino, Italy.
authors: R
authors: R
authors: Minelli
authors: L
authors: L
authors: Serpe
authors: P
authors: P
authors: Pettazzoni
authors: V
authors: V
authors: Minero
authors: G
authors: G
authors: Barrera
authors: Cl
authors: C
authors: Gigliotti
authors: R
authors: R
authors: Mesturini
authors: A C
authors: AC
authors: Rosa
authors: P
authors: P
authors: Gasco
authors: N
authors: N
authors: Vivenza
authors: E
authors: E
authors: Muntoni
authors: R
authors: R
authors: Fantozzi
authors: U
authors: U
authors: Dianzani
authors: G P
authors: GP
authors: Zara
authors: C
authors: C
authors: Dianzani
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002788:Cholesterol Esters / Q000494:pharmacology*; D003110:Colonic Neoplasms; D004337:Drug Carriers / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053758:Nanoparticles
chemical_list: D000970:Antineoplastic Agents; D002788:Cholesterol Esters; D004337:Drug Carriers; C092211:cholesteryl butanoate