title: TiO2 nanotubes as drug nanoreservoirs for the regulation of mobility and differentiation of mesenchymal stem cells.
abstract: The extracellular microenvironment plays a key role in the regulation of cellular behavior. To mimic the natural extracellular microenvironment, TiO(2) nanotube (TNT) arrays as drug nanoreservoirs for loading of bone morphogenetic protein 2 (BMP2) were constructed on titanium substrates and then covered with multilayered coatings of gelatin/chitosan (Gel/Chi) for controlled drug release. The multilayered coatings were constructed via a spin-assisted layer-by-layer assembly technique. The successful fabrication of this system was monitored by field emission scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy and contact angle measurements. Multilayered coating with Gel/Chi retained the drug bioactivity and release properties, which were revealed by superoxide dismutase activity measurement. In addition, cytoskeleton observation and wound healing assay confirmed that BMP2-loaded and multilayer-coated TNT arrays were able to stimulate motogenic responses of mesenchymal stem cells (MSCs). More importantly, the system demonstrated that it was capable of promoting the osteoblastic differentiation of MSCs. This study may have potential impact on the development of bone implants for enhanced bone osseointegration.
authors: Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, People's Republic of China.
authors: Yan
authors: Y
authors: Hu
authors: Kaiyong
authors: K
authors: Cai
authors: Zhong
authors: Z
authors: Luo
authors: Dawei
authors: D
authors: Xu
authors: Daichao
authors: D
authors: Xie
authors: Yuran
authors: Y
authors: Huang
authors: Weihu
authors: W
authors: Yang
authors: Peng
authors: P
authors: Liu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry*; D016348:Osseointegration / Q000502:physiology; D019736:Prostheses and Implants; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; C009495:titanium dioxide; D005780:Gelatin; D048271:Chitosan; D014025:Titanium; D013482:Superoxide Dismutase