title: Extraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside.
abstract: This study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity.
authors: Research Institute of Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors: Akira
authors: A
authors: Hashimoto
authors: Kenji
authors: K
authors: Shimono
authors: Yoshiki
authors: Y
authors: Horikawa
authors: Tsukasa
authors: T
authors: Ichikawa
authors: Masahisa
authors: M
authors: Wada
authors: Tomoya
authors: T
authors: Imai
authors: Junji
authors: J
authors: Sugiyama
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis / Q000648:ultrastructure; D003902:Detergents / Q000737:chemistry*; D004072:Digitonin; D041822:Gluconacetobacter xylinus / Q000201:enzymology*; D005960:Glucosides / Q000737:chemistry*; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D017830:Octoxynol; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003902:Detergents; D005960:Glucosides; D017830:Octoxynol; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase; D004072:Digitonin