title: The necessity for the coating of perfluorodecalin-filled poly(lactide-co-glycolide) microcapsules in the presence of physiological cholate concentrations: Tetronic-908 as an exemplary polymeric surfactant.
abstract: Recently, we demonstrated that biodegradable poly(lactide-co-glycolide) (PLGA) micro- and nanocapsules with a liquid content of perfluorodecalin are principally useful for the development of artificial oxygen carriers. In order to solve a decisive and well-known problem with PLGA microcapsules, i.e. the spontaneous agglomeration of the capsules after depletion of the emulsifying agent (i.e. cholate), coating with the ABA block copolymer, Tetronic-908 was studied. After Tetronic-908 treatment at concentrations that were harmless to cultured cells, the clustering of the microcapsules was prevented, the adsorption of opsonins was decreased and the attachment to cells was inhibited, but the oxygen transport capacity of PLGA microcapsules was even increased. The present data clearly show that perfluorodecalin-filled PLGA microcapsules must be coated before decreasing the emulsifying agent cholate to physiological concentrations, in order to develop a solution that has the capabilities to function as a potential artificial oxygen carrier suspension.
authors: Institute of Physiological Chemistry, University Hospital Essen, Hufelandstrasse 55, Essen, Germany. michael.kirsch@uni-duisburg-essen.de
authors: M
authors: M
authors: Kirsch
authors: T
authors: T
authors: Bramey
authors: I N
authors: IN
authors: Waack
authors: F
authors: F
authors: Petrat
authors: C
authors: C
authors: Mayer
authors: H
authors: H
authors: de Groot
mesh_terms: D000327:Adsorption; D000818:Animals; D002214:Capsules; D002460:Cell Line; D019826:Cholic Acid / Q000032:analysis*; D005466:Fluorocarbons / Q000737:chemistry*; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002214:Capsules; D005466:Fluorocarbons; C116176:PEO-PPO-PEO; D011108:Polymers; D011409:Propylene Glycols; D013501:Surface-Active Agents; D011098:Polyglactin 910; D011092:Polyethylene Glycols; C008805:perfluorodecalin; D019826:Cholic Acid