title: [Role of three ELISA tests using promastigote homogenates of Leishmania braziliensis, L. amazonensis and L. guyanensis in the diagnosis of tegumentary leishmaniasis].
abstract: It is important to know whether the variability of species of Leishmania parasites circulating in a region affects the performance of the ELISA test for the diagnosis of leishmaniasis. Therefore, the aim of this study was to analyze the reactivity of the ELISA using homogenates of promastigotes of Leishmania (V.) braziliensis (ELISAb), Leishmania (L) amazonensis (ELISAa) and Leishmania (V.) guyanensis (ELISAg) against different sera groups. Samples from individuals with cutaneous leishmaniasis (n = 37), mucocutaneous leishmaniasis (n = 8), healthy controls (n = 52), persons infected with Trypanosoma cruzi (n = 11) and mixed infections (n = 14) were included in the study. We calculated sensitivities, specificities, cut offs, and predictive values for the three tests and compared them using ANOVA, kappa index, ROC curves comparison, and confidence intervals calculated by the bootstrap method. Significant differences were found when comparing the OD levels of sera from patients with cutaneous leishmaniasis against healthy controls, but there were no differences when comparing the different ELISAs. The sensitivities calculated for ELISAb and ELISAa were 84.6 and of 88.5% for ELISAg, while the value of specificity for the three tests was 96.2. The kappa index (0.87) and comparison of ROC curves showed similar performance for the three ELISAs (p = 0.225). The high reactivity obtained for these ELISAs in sera of patients with mucocutaneous leishmaniasis indicates this test as an important complement in the diagnosis of the disease.
authors: Instituto de Investigaciones en Enfermedades Tropicales (IIET), Sede Regional Orán. jgil@conicet.gov.ar
authors: José F
authors: JF
authors: Gil
authors: Carlos L
authors: CL
authors: Hoyos
authors: Rubén O
authors: RO
authors: Cimino
authors: Alejandro J
authors: AJ
authors: Krolewiecki
authors: Inés
authors: I
authors: López Quiroga
authors: Silvana P
authors: SP
authors: Cajal
authors: Marisa
authors: M
authors: Juárez
authors: María F
authors: MF
authors: García Bustos
authors: María C
authors: MC
authors: Mora
authors: Jorge D
authors: JD
authors: Marco
authors: Julio R
authors: JR
authors: Nasser
mesh_terms: D000704:Analysis of Variance; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology; D016001:Confidence Intervals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007891:Leishmania / Q000276:immunology*; D007892:Leishmania braziliensis / Q000276:immunology; D018334:Leishmania guyanensis / Q000276:immunology; D007894:Leishmania mexicana / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000276:immunology; D007897:Leishmaniasis, Mucocutaneous / Q000175:diagnosis / Q000276:immunology; D015800:Protozoan Proteins / Q000097:blood*; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi / Q000737:chemistry
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins