title: Enterococcus rivorum sp. nov., from water of pristine brooks.
abstract: A significant number of Enterococcus strains from pristine waters of two brooks in Finland formed a distinct cluster on the basis of whole-cell protein fingerprinting by one-dimensional SDS-PAGE. The strains shared the following characteristics. Cells were ovoid, Gram-positive-staining and non-spore-forming, appearing singly or in pairs or chains. They were facultatively anaerobic and catalase-negative. Growth in broth containing 6.5 % NaCl or at 45 °C was weak or absent. Production of D antigen was variable. The strains tolerated 60 °C for 30 min, 40 % bile and tellurite, hydrolysed aesculin strongly and gelatin weakly, produced no acid from hippurate and did not reduce it, grew weakly at 10 °C, showed a strong reaction for the Voges-Proskauer test and produced acid from methyl α-d-glucoside, mannitol, sorbitol and sucrose, with weak or no production of acid from methyl α-d-mannoside, l-arabinose, gluconate and l-xylose. Several of the strains were selected for identification on the basis of sequencing of almost the whole 16S rRNA gene and partial atpA and pheS genes and of (GTG)(5)-PCR fingerprints. Partial atpA and pheS gene sequencing was also performed for those type strains of Enterococcus species without available sequences in the database. The pristine brook isolates formed a novel species, for which the name Enterococcus rivorum sp. nov. (type strain S299(T) = HAMBI 3055(T) = LMG 25899(T) = CCM 7986(T)) is proposed. On the basis of 16S rRNA gene sequence similarity, E. rivorum sp. nov. is related to the Enterococcus faecalis genogoup. It is distinguished from described Enterococcus species on the basis of 16S rRNA, atpA and pheS gene sequences and whole-cell protein and (GTG)(5)-PCR fingerprints. It is most closely related to E. faecalis, but DNA-DNA hybridization confirms it to represent a novel species.
authors: Finnish Environment Institute, Ecosystem Change, PO Box 140, FI-00251 Helsinki, Finland.
authors: R Maarit
authors: RM
authors: Niemi
authors: Finnish Environment Institute, Ecosystem Change, PO Box 140, FI-00251 Helsinki, Finland.
authors: Tuula
authors: T
authors: Ollinkangas
authors: Institute of Biotechnology, University of Helsinki, PO Box 56, FI-00014 Helsinki, Finland.
authors: Lars
authors: L
authors: Paulin
authors: Masaryk University, Faculty of Science, Department of Experimental Biology, Czech Collection of Microorganisms (CCM), Tvrdého 14, Brno 60200, Czech Republic.
authors: Pavel
authors: P
authors: Švec
authors: Laboratory of Bacteriology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors: Peter
authors: P
authors: Vandamme
authors: Department of Food and Environmental Sciences, Division of Microbiology, University of Helsinki, PO Box 56, FI-00014 Helsinki, Finland.
authors: Antti
authors: A
authors: Karkman
authors: Masaryk University, Faculty of Science, Department of Experimental Biology, Czech Collection of Microorganisms (CCM), Tvrdého 14, Brno 60200, Czech Republic.
authors: Marcel
authors: M
authors: Kosina
authors: Department of Food and Environmental Sciences, Division of Microbiology, University of Helsinki, PO Box 56, FI-00014 Helsinki, Finland.
authors: Kristina
authors: K
authors: Lindström
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005387:Finland; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S