title: [The influence of stress factors on liver function and lipid metabolism in an animal model of arterial hypertension].
abstract: OBJECTIVE
  to investigate the influence of some stress factors on hepatic function and lipid metabolism in an experimental-induced hypertension.
  
  
  METHODS
  The experiment was carried out on Wistar rats, treated intraperitoneally, for 4 weeks, as follows: Group 1: saline solution (0.5ml/100g weight); Group 2: saline solution + stress; Group 3: Endotelin-1; Group 4: Endotelin-1 + stress; Group 5: Endotelin-1 + cholesterol diet (0.2g/kbw/day); Group 6: Endotelin-1 + cholesterol diet + stress. Endotelin-1 0.25nmol/kbw was initially administered, followed by 0.5nmol/kbw 15 min later, 2 times/week. Stress-inducing factors were immobilization and water immersion. In the 28th day of the experiment blood pressure was measured and blood samples were taken from retro-orbitary plexus to assess glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactic dehydrogenase (LDH) activity, total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides levels.
  
  
  RESULTS
  Using immobilization and water immersion as chronic stress, our study proved an increase of GOT, GTP and total and LDL cholesterol in rats with endotelin-1-induced hypertension.
  
  
  CONCLUSIONS
  In this animal model of endothelin-1-induced hypertension the blood pressure increased significantly under chronic exposure to stress, reaching the highest values when associating stress and experimentally induced dyslipidemia.
authors: Universităţii de Medicină şi Farmacie "Gr. T. Popa" Iaşi.
authors: C
authors: C
authors: Diaconu
authors: Liliana
authors: L
authors: Tarţău
authors: Cătălina-Elena
authors: CE
authors: Lupuşoru
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D001794:Blood Pressure; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000008:administration & dosage / Q000009:adverse effects*; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D007101:Immersion; D008099:Liver / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological; D013315:Stress, Psychological; D014280:Triglycerides / Q000097:blood; D014867:Water
chemical_list: D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D019332:Endothelin-1; D014280:Triglycerides; D014867:Water; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase