title: Age- and chamber-specific differences in oxidative stress after ischemic injury.
abstract: Each year, tens of thousands of children undergo cardiopulmonary bypass (CPB) to correct congenital heart defects. Although necessary for surgery, CPB involves stopping the heart and exposing it to ischemic conditions. On reoxygenation, the heart can experience effects similar to that of acute myocardial infarction. Although much is known about adult injury, little is known about the effects of global ischemia on newborn ventricles. We studied newborn (2 to 4 days old) and adult (>8 weeks old) rabbit hearts subjected to ischemia-reperfusion (30 min of ischemia and 60 min of reperfusion). Our data demonstrated chamber- and age-specific changes in oxidative stress. During ischemia, hydrogen peroxide (H(2)O(2)) increased in both right-ventricular (RV) and left-ventricular (LV) myocytes of the newborn, although only the RV change was significant. In contrast, there was no significant increase in H(2)O(2) in either RV or LV myocytes of adults. There was a fivefold increase in H(2)O(2) formation in newborn RV myocytes compared with adults (P = 0.006). In whole-heart tissue, superoxide dismutase activity increased from sham versus ischemia in the left ventricle of both adult and newborn hearts, but it was increased only in the right ventricle of the newborn heart. Catalase activity was significantly increased after ischemia in both adult ventricles, whereas no increase was seen in newborn compared with sham hearts. In addition, catalase levels in newborns were significantly lower, indicating less scavenging potential. Nanoparticle-encapsulated ebselen, given as an intracardiac injection into the right or left ventricle of newborn hearts, significantly increased functional recovery of developed pressure only in the right ventricle, indicating the potential for localized antioxidant therapy during and after pediatric surgical procedures.
authors: Wallace H. Coulter Department of Biomedical Engineering at Emory University, Georgia Institute of Technology, Atlanta, GA 30322, USA.
authors: E Bernadette
authors: EB
authors: Cabigas
authors: Guoliang
authors: G
authors: Ding
authors: Tao
authors: T
authors: Chen
authors: Talib B
authors: TB
authors: Saafir
authors: Karl D
authors: KD
authors: Pendergrass
authors: Mary B
authors: MB
authors: Wagner
authors: Michael E
authors: ME
authors: Davis
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000008:administration & dosage; D001393:Azoles / Q000008:administration & dosage; D002374:Catalase / Q000096:biosynthesis; D006352:Heart Ventricles / Q000378:metabolism / Q000503:physiopathology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D054833:Isoindoles; D017202:Myocardial Ischemia; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D053758:Nanoparticles; D016566:Organoselenium Compounds / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects*; D011817:Rabbits; D020127:Recovery of Function
chemical_list: D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; C042986:ebselen; D006861:Hydrogen Peroxide; D002374:Catalase