title: Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene.
abstract: Adulteration of high quality meat and meat products with their inferior/cheaper counterparts is a problem in the meat industry. The present study investigated the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene for identification of the origin of meats. PCR-RFLP was applied for species identification of beef, buffalo meat, mutton and chevon. PCR amplification yielded a 456-bp fragment in each of these species. The amplicons were digested with AluI, HhaI, ApoI and BspTI restriction enzymes resulting in a pattern that could identify and differentiate each of the above species. This technique did not yield satisfactory results with meat mixtures/meats. However, consistent results were obtained with both fresh and processed meat samples.
authors: Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India.
authors: P S
authors: PS
authors: Girish
authors: A S R
authors: AS
authors: Anjaneyulu
authors: K N
authors: KN
authors: Viswas
authors: B M
authors: BM
authors: Shivakumar
authors: M
authors: M
authors: Anand
authors: M
authors: M
authors: Patel
authors: B
authors: B
authors: Sharma