title: Enhanced antitumor activity of epirubicin combined with cerulenin in             osteosarcoma.
abstract: Osteosarcoma (OS) is the most common primary malignant neoplasm in children and adolescents. Epirubicin is one of the chemotherapeutic agents currently used for the treatment of OS; however, the efficacy of chemotherapy is hampered by the acquired drug resistance of OS. Cerulenin, an inhibitor of fatty acid synthase, has been defined as a candidate tumor suppressor. In this study, we explored the combined effect of cerulenin plus the chemotherapy drug, epirubicin, on human OS U2-OS cells in vitro and in vivo. We demonstrated that cerulenin plus epirubicin induced synergistic growth inhibition and enhanced apoptosis in U2-OS cells. We also demonstrated that cerulenin plus epirubicin synergistically suppressed tumor growth in subcutaneously xenografted U2-OS cells in athymic nude mice. Our results indicate that cerulenin enhances the anti-OS effects of epirubicin in vivo and in vitro.
authors: Graduate School of Southern Medical University, Guangzhou, Guangdong, PR China.
authors: Z L
authors: ZL
authors: Liu
authors: G
authors: G
authors: Wang
authors: Y
authors: Y
authors: Shu
authors: P A
authors: PA
authors: Zou
authors: Y
authors: Y
authors: Zhou
authors: Q S
authors: QS
authors: Yin
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002569:Cerulenin / Q000494:pharmacology / Q000627:therapeutic use*; D004357:Drug Synergism; D015251:Epirubicin / Q000494:pharmacology / Q000627:therapeutic use*; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012516:Osteosarcoma / Q000188:drug therapy*; D014183:Transplantation, Heterologous
chemical_list: D000903:Antibiotics, Antineoplastic; D002569:Cerulenin; D015251:Epirubicin; D064429:Fatty Acid Synthases