title: Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection.
abstract: Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses.
authors: Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
authors: Nuno
authors: N
authors: Carinhas
authors: Aaron Mark
authors: AM
authors: Robitaille
authors: Suzette
authors: S
authors: Moes
authors: Manuel José Teixeira
authors: MJ
authors: Carrondo
authors: Paul
authors: P
authors: Jenoe
authors: Rui
authors: R
authors: Oliveira
authors: Paula Marques
authors: PM
authors: Alves
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000302:isolation & purification*; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004734:Energy Metabolism; D019476:Insect Proteins / Q000378:metabolism*; D007553:Isotope Labeling; D040901:Proteomics; D018411:Spodoptera / Q000254:growth & development / Q000378:metabolism* / Q000821:virology
chemical_list: D019476:Insect Proteins