title: Purification of BAC DNA for high-efficiency transgenesis.
abstract: An unresolved bottleneck in bacterial artificial chromosome (BAC) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAC DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl(2) centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAC DNA that generates transgenic founders with up to 80% efficiency.
authors: Jules Stein Eye Institute, UCLA, University of California, Los Angeles, CA 90095-700019, USA. rkganga@jsei.ucla.edu
authors: Rajendra K
authors: RK
authors: Gangalum
authors: Zhe
authors: Z
authors: Jing
authors: Yoshiko
authors: Y
authors: Nagaoka
authors: Meisheng
authors: M
authors: Jiang
authors: Suraj P
authors: SP
authors: Bhat
authors: Suraj P
authors: SP
authors: Bhat
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry* / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D003956:Dialysis / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D018014:Gene Transfer Techniques / Q000191:economics*; D051379:Mice; D008822:Mice, Transgenic; D019076:Transgenes
chemical_list: D004247:DNA