title: Spleen-resident CD4+ and CD4- CD8α- dendritic cell subsets differ in their ability to prime invariant natural killer T lymphocytes.
abstract: One important function of conventional dendritic cells (cDC) is their high capacity to capture, process and present Ag to T lymphocytes. Mouse splenic cDC subtypes, including CD8α(+) and CD8α(-) cDC, are not identical in their Ag presenting and T cell priming functions. Surprisingly, few studies have reported functional differences between CD4(-) and CD4(+) CD8α(-) cDC subsets. We show that, when loaded in vitro with OVA peptide or whole protein, and in steady-state conditions, splenic CD4(-) and CD4(+) cDC are equivalent in their capacity to prime and direct CD4(+) and CD8(+) T cell differentiation. In contrast, in response to α-galactosylceramide (α-GalCer), CD4(-) and CD4(+) cDC differentially activate invariant Natural Killer T (iNKT) cells, a population of lipid-reactive non-conventional T lymphocytes. Both cDC subsets equally take up α-GalCer in vitro and in vivo to stimulate the iNKT hybridoma DN32.D3, the activation of which depends solely on TCR triggering. On the other hand, and relative to their CD4(+) counterparts, CD4(-) cDC more efficiently stimulate primary iNKT cells, a phenomenon likely due to differential production of co-factors (including IL-12) by cDC. Our data reveal a novel functional difference between splenic CD4(+) and CD4(-) cDC subsets that may be important in immune responses.
authors: Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Lille, France.
authors: Emilie
authors: E
authors: Bialecki
authors: Elodie
authors: E
authors: Macho Fernandez
authors: Stoyan
authors: S
authors: Ivanov
authors: Christophe
authors: C
authors: Paget
authors: Josette
authors: J
authors: Fontaine
authors: Fabien
authors: F
authors: Rodriguez
authors: Luc
authors: L
authors: Lebeau
authors: Christophe
authors: C
authors: Ehret
authors: Benoit
authors: B
authors: Frisch
authors: François
authors: F
authors: Trottein
authors: Christelle
authors: C
authors: Faveeuw
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D005699:Galactosylceramides / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology* / Q000276:immunology
chemical_list: D016827:CD8 Antigens; C431698:CD8alpha antigen; D005699:Galactosylceramides; C493154:alpha-galactosylceramide