title: A pilot study on the effect of short-term consumption of a polyphenol rich drink on biomarkers of coronary artery disease defined by urinary proteomics.
abstract: Polyphenol rich diets have been associated with a reduced risk of cardiovascular disease. We examined the effect of a polyphenol rich (P-R) drink on biomarkers assessed by urinary proteomics. Thirty nine middle aged and overweight subjects were randomized to P-R drink (n = 20) or placebo (n = 19) in addition to their normal diet. After two weeks urine samples were obtained for assessment of the urinary proteome using capillary electrophoresis coupled to a mass spectrometer. A total of 93 polypeptides were found to be candidates for differential distribution with a nominal p-value <0.05, though these differences did not reach significance when multiple testing was accounted for. Sequences were determined in 19 of these demonstrating that they originate from alpha-1 antitrypsin, collagens, fibrinogen alpha and IgG kappa. Levels of 27 polypeptides were greater than 4-fold different between the two groups. Of these, 7 were previously found to be part of a coronary artery disease (CAD) specific urinary biomarker pattern. Their direction of expression was closer to the healthy state in the P-R drink group and closer to CAD state in the placebo group. Our data suggest that the P-R drink may have beneficial effects on urinary biomarkers of CAD. The data encourage the planning of future prospective studies, aimed at investigating significant effects of polyphenol rich dietary products.
authors: Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors: W
authors: W
authors: Mullen
authors: J
authors: J
authors: Gonzalez
authors: J
authors: J
authors: Siwy
authors: J
authors: J
authors: Franke
authors: N
authors: N
authors: Sattar
authors: A
authors: A
authors: Mullan
authors: S
authors: S
authors: Roberts
authors: C
authors: C
authors: Delles
authors: H
authors: H
authors: Mischak
authors: A
authors: A
authors: Albalat
mesh_terms: D000368:Aged; D001628:Beverages; D015415:Biomarkers / Q000652:urine*; D003327:Coronary Disease / Q000517:prevention & control / Q000652:urine*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D050177:Overweight; D010865:Pilot Projects; D010919:Placebos; D059808:Polyphenols / Q000008:administration & dosage*; D040901:Proteomics
chemical_list: D015415:Biomarkers; D010919:Placebos; D059808:Polyphenols