title: In vivo evaluation of the biocompatibility of surface modified hemodialysis polysulfone hollow fibers in rat.
abstract: Polysulfone (Psf) hollow fiber membranes (HFMs) have been widely used in blood purification but their biocompatibility remains a concern. To enhance their biocompatibility, Psf/TPGS (d-α-tocopheryl polyethylene glycol 1000 succinate) composite HFMs and 2-methacryloyloxyethyl phosphorylcholine (MPC) coated Psf HFMs have been prepared. They have been evaluated for in vivo biocompatibility and graft acceptance and compared with sham and commercial membranes by intra-peritoneal implantation in rats at day 7 and 21. Normal body weights, tissue formation and angiogenesis indicate acceptance of implants by the animals. Hematological observations show presence of post-surgical stress which subsides over time. Serum biochemistry results reveal normal organ function and elevated liver ALP levels at day 21. Histological studies exhibit fibroblast recruitment cells, angiogenesis and collagen deposition at the implant surface indicating new tissue formation. Immuno-histochemistry studies show non-activation of MHC molecules signifying biocompatibilty. Additionally, Psf/TPGS exhibit most favorable tissue response as compared with other HFMs making them the material of choice for HFM preparation for hemodialysis applications.
authors: Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, India.
authors: Ganpat J
authors: GJ
authors: Dahe
authors: Sachin S
authors: SS
authors: Kadam
authors: Siddharth S
authors: SS
authors: Sabale
authors: Dattatray P
authors: DP
authors: Kadam
authors: Laxman B
authors: LB
authors: Sarkate
authors: Jayesh R
authors: JR
authors: Bellare
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D020007:Implants, Experimental; D008422:Materials Testing; D008567:Membranes, Artificial; D011108:Polymers / Q000627:therapeutic use*; D051381:Rats; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones / Q000627:therapeutic use*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700