title: Prokaryotic expression of woodchuck cytotoxic T lymphocyte antigen 4 (wCTLA-4) and preparation of polyclonal antibody to wCTLA-4.
abstract: Cytotoxic T lymphocyte antigen 4 (CTLA-4) is an inhibitory T cell receptor predominately expressed on activated T cells and plays an important role in regulation of specific T cell responses to viral infection. The woodchuck model is an informative animal model for hepatitis B virus (HBV) infection. In this study, the extracellular region of woodchuck CTLA-4 (wCTLA-4) was cloned and the fusion protein of GST-wCTLA-4 was expressed and purified. Polyclonal antibody against GST-wCTLA-4 (anti-GST-wCTLA-4) was prepared. The full length wCTLA-4 protein expressed in transfected baby hamster kidney cells was detected by anti-GST-wCTLA-4 in western blot analysis and immunofluorescence staining. Anti-GST-wCTLA-4 provides a useful tool to study the role of CTLA-4 in T-cell response in the woodchuck model. Further, the blocking of CTLA-4 with anti-GST-wCTLA-4, as a novel therapy approach for chronic hepatitis B virus infection, could be studied in woodchuck model now.
authors: Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, China.
authors: Yinke
authors: Y
authors: Yang
authors: Baoju
authors: B
authors: Wang
authors: Dongliang
authors: D
authors: Yang
authors: Mengji
authors: M
authors: Lu
authors: Yang
authors: Y
authors: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D060908:CTLA-4 Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D017919:Hepatitis B Virus, Woodchuck / Q000276:immunology / Q000472:pathogenicity; D008392:Marmota / Q000235:genetics / Q000276:immunology* / Q000821:virology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
chemical_list: D060908:CTLA-4 Antigen; D006510:Hepatitis B Antibodies; D011993:Recombinant Fusion Proteins