title: A multiplex SNP assay for the dissection of human Y-chromosome haplogroup O representing the major paternal lineage in East and Southeast Asia.
abstract: The majority of human Y chromosomes in men from East and Southeast Asia, and a considerable proportion of Oceanian men, especially those from Remote Oceania, belong to haplogroup O, characterized by a 5-bp deletion known as M175 (rs2032678). Recent advances in Y-SNP (single-nucleotide polymorphism) discovery have substantially improved the phylogenetic resolution of haplogroup O sublineages. By taking advantage of this recent knowledge, we hereby introduce a sensitive Y-SNP multiplex genotyping assay for the dissection of haplogroup O into its most significant sublineages. The multiplex assay thus provides an efficient way to infer patrilineal biogeographic ancestry in males of Asian/Oceanian patrilineal descent, and is suitable for applications in human population genetics, anthropological, genealogical, as well as forensic studies.
authors: Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands. m.vanoven@erasmusmc.nl
authors: Mannis
authors: M
authors: van Oven
authors: Nathalie
authors: N
authors: van den Tempel
authors: Manfred
authors: M
authors: Kayser
mesh_terms: D001210:Asia, Southeastern; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005202:Asia, Eastern; D005220:Fathers; D005819:Genetic Markers; D005828:Genetics, Population; D060005:Genotyping Techniques / Q000379:methods*; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012015:Reference Standards
chemical_list: D005819:Genetic Markers