title: Comparison between TEWL and laser scanning microscopy measurements for the in vivo characterization of the human epidermal barrier.
abstract: The analysis of the skin barrier properties is important in various fields of medical treatment and cosmetology. The development and improvement of topically applied substances require an objective analysis of the skin barrier characteristics. Transepidermal water loss (TEWL) measurement is the standard method to characterize epidermal barrier function. The most important disadvantage of this method though, is that it can be affected by different exogenous and endogenous factors, e.g. water content of the applied formulation and room temperature. In the present study, TEWL measurements are compared to laser scanning microscopic (LSM) measurements, concerning the use of these two methods for the non-invasive in vivo characterization of the epidermal barrier function. The investigations were performed prior and subsequent to treatment of dry skin with a gel mixture, developed for skin treatment after radiotherapy for cancer. The present results indicate that in vivo laser scanning microscopy is an appropriate method for the characterization of the skin barrier structure without interference by external factors.
authors: Hospital A. Sygros, Department of Dermatology, University of Athens, Athens, Greece. siniavergou@hotmail.com
authors: Theognosia
authors: T
authors: Vergou
authors: Sabine
authors: S
authors: Schanzer
authors: Heike
authors: H
authors: Richter
authors: Ragna
authors: R
authors: Pels
authors: Gisela
authors: G
authors: Thiede
authors: Alexa
authors: A
authors: Patzelt
authors: Martina C
authors: MC
authors: Meinke
authors: Wolfram
authors: W
authors: Sterry
authors: Joachim W
authors: JW
authors: Fluhr
authors: Jürgen
authors: J
authors: Lademann
mesh_terms: D000328:Adult; D004817:Epidermis / Q000473:pathology*; D005260:Female; D005782:Gels / Q000737:chemistry; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods*; D008875:Middle Aged; D009821:Oils / Q000737:chemistry; D012107:Research Design; D012867:Skin / Q000473:pathology*; D012879:Skin Physiological Phenomena; D014867:Water / Q000737:chemistry; D014870:Water Loss, Insensible
chemical_list: D005782:Gels; D009821:Oils; D014867:Water