title: Evaluation of possible antioxidant and anticonvulsant effects of the ethyl acetate fraction from Platonia insignis Mart. (Bacuri) on epilepsy models.
abstract: The aim of present study was to examine the effects of the ethyl acetate fraction (EAF) from Platonia insignis on lipid peroxidation level, nitrite formation, and superoxide dismutase and catalase activities in rat striatum prior to pilocarpine-induced seizures as well as to explore its anticonvulsant activity in adult rats prior to pentylenetetrazole (PTZ)- and picrotoxin (PIC)-induced seizures. Wistar rats were treated with vehicle, atropine (25mg/kg), EAF (0.1, 1, and 10mg/kg), pilocarpine (400mg/kg, P400 group), PTZ (60 mg/kg, PTZ group), PIC (8 mg/kg, PIC group), atropine+P400, EAF+P400, EAF+PTZ, or EAF+PIC. Significant decreases in number of crossings and rearings were observed in the P400 group. The EAF 10+P400 group also had significant increases in these parameters. In addition, in rats treated with P400, there were significant increases in lipid peroxidation and nitrite levels; however, there were no alterations in SOD and catalase activities. In the EAF 10+P400 group, lipid peroxidation and nitrite levels significantly decreased and SOD and catalase activities significantly increased after pilocarpine-induced seizures. Additionally, effects of the EAF were evaluated in PTZ and PIC models. EAF did not increase the latency to development of convulsions induced with PTZ and PIC at the doses tested. Our findings strongly support the hypothesis that EAF does not have anticonvulsant activity in the different models of epilepsy studied. Our results indicate that in the in vivo model of pilocarpine-induced seizures, EAF has antioxidant activity, but not anticonvulsant properties at the doses tested.
authors: Laboratory of Genetic Toxicology, Postgraduate Program in Genetic and Applied Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil.
authors: Joaquim Soares da Costa
authors: JS
authors: Júnior
authors: Antonia Amanda C
authors: AA
authors: de Almeida
authors: Adriana da Rocha
authors: Ada R
authors: Tomé
authors: Antonia Maria das Graças Lopes
authors: AM
authors: Citó
authors: Jenifer
authors: J
authors: Saffi
authors: Rivelilson Mendes
authors: RM
authors: de Freitas
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004339:Drug Compounding; D004347:Drug Interactions; D004827:Epilepsy / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008401:Gas Chromatography-Mass Spectrometry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D002365:Manihot / Q000737:chemistry*; D009043:Motor Activity / Q000187:drug effects; D018721:Muscarinic Agonists / Q000633:toxicity; D010433:Pentylenetetrazole / Q000633:toxicity; D008517:Phytotherapy / Q000379:methods*; D010852:Picrotoxin / Q000633:toxicity; D010862:Pilocarpine / Q000633:toxicity; D028321:Plant Preparations / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000085:Acetates; D000927:Anticonvulsants; D000975:Antioxidants; D002374:Catalase; D018721:Muscarinic Agonists; D010433:Pentylenetetrazole; D010852:Picrotoxin; D010862:Pilocarpine; D028321:Plant Preparations; D013482:Superoxide Dismutase; C007650:ethyl acetate