title: [Study of quantitative detection of circulating DNA in the plasma of patients with cervical lesion].
abstract: OBJECTIVE
  To quantitatively detect circulating DNA levels in the plasma of patients withcervical lesion and to determine the value for diagnosis of cervical lesion and cervical cancer.
  
  
  METHODS
  Preoperative blood samples were collected from 53 cases of low-grade lesions, 49 cases of high-grade lesions, 44 cases of cervical invasive cancer and 70 cases of healthy women. Plasma DNA was extracted by magnetic bead method (BILATEST DNA kit). The quantity of plasma DNA was determined by duplex real-time quantitative PCR.
  
  
  RESULTS
  Median plasma DNA level of invasive cervical cancer patients was 61.59 mg/L (32.06-162.16 mg/L), which was significantly higher than that of healthy women [16.35 mg/L (11.98-22.71 mg/L), P<0.01]. Among invasive cervical cancer patients, median plasma DNA level of squamous carcinoma patients was slightly higher than that of adenocarcinoma (50.43 versus 47.31 mg/L, P>0.05). Median plasma DNA level of stage I patients was lower than that of stage II-III patients (46.02 versus 71.35 mg/L, P<0.05).
  
  
  CONCLUSIONS
  Quantitatively detecting plasma circulating DNA may be with some application prospect in the diagnosis of cervical diseases.
authors: Department of Laboratory Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors: Hong
authors: H
authors: Wang
authors: Shi-yang
authors: SY
authors: Pan
authors: Jian
authors: J
authors: Xu
authors: Mei-juan
authors: MJ
authors: Zhang
authors: Dan
authors: D
authors: Chen
authors: Wen-ying
authors: WY
authors: Xia
authors: Ya-chun
authors: YC
authors: Lu
authors: Yan
authors: Y
authors: Geng
authors: Bai
authors: B
authors: Jin
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000175:diagnosis; D002578:Uterine Cervical Dysplasia / Q000097:blood* / Q000175:diagnosis
chemical_list: D014408:Biomarkers, Tumor; D004247:DNA