title: Bioactive phenolic constituents from the seeds of Pharbitis nil.
abstract: Two new lignans, termed pharsyringaresinol (1) and pharbilignoside (2), a new phenylethanoid glycoside, termed pharbiniloside (3), and 22 known compounds, were isolated from the ethanol extract of the seeds of Pharbitis nil. The structures of the new compounds (1-3) were determined on the basis of spectroscopic analyses, including 2D-NMR and circular dichroism (CD) spectroscopy studies. Among the isolates, compounds 2, 11, 12, and 24 exhibited significant cytotoxicity against human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15) with IC(50) values ranging from 8.07 to 28.30 µM. In addition, compounds 11, 12 and 24 potently inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells, a microglia cells with IC(50) values ranging from 14.7 to 19.9 µM.
authors: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440–746, Korea.
authors: Ki Hyun
authors: KH
authors: Kim
authors: Sang Keun
authors: SK
authors: Ha
authors: Sang Un
authors: SU
authors: Choi
authors: Sun Yeou
authors: SY
authors: Kim
authors: Kang Ro
authors: KR
authors: Lee
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D049915:Ipomoea nil / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism; D012639:Seeds / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D008070:Lipopolysaccharides; C570333:pharsyringaresinol; D009569:Nitric Oxide