title: Isolation and identification of endophytic fungi in roots of nine Holcoglossum plants (Orchidaceae) collected from Yunnan, Guangxi, and Hainan provinces of China.
abstract: Holcoglossum is one of the smaller genera of Orchidaceae, mainly distributed in southwest China. Some members of this genus as well as H. rupestre and H. flavescens are endemic and rare Chinese orchids. As far as we know, little work has been done concerning the relationships between the Holcoglossum plants and endophytic microorganisms. In this study, 46 culturable fungal endophytes were isolated and identified from roots of nine Holcoglossum plants collected from Yunnan, Guangxi, and Hainan provinces of China based on molecular techniques. The results showed that all strains belonged to four classes, i.e., Sordariomycetes (41.30%), Dothideomycetes (36.96%), Agaricomycetes (17.39%), Leotiomycetes (4.35%). Thirty-six strains were identified at the genus level, including Alternaria, Cladosporium, Clonostachys, Colletotrichum, Cosmospora, Cryptosporiopsis, Cylindrocarpon, Didymella, Epulorhiza (Anamorphic Tulasnella), Fusarium, Myrmecridium, Leptosphaeria, Paraconiothyrium, Phomopsis, Pyrenochaeta, and Stephanonectria. Fusarium and Epulorhiza (Anamorphic Tulasnella) were the dominant fungal endophytes. Some orchids mycorrhizal fungi as well as Tulasnella calospora and Epulorhiza sp. were found in roots. This is the first report concerning endophytic fungi from Holcoglossum plants (Orchidaceae), suggesting that endophytic fungi in Holcoglossum plants are very abundant.
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian, Beijing 100193, China.
authors: Xiao-Ming
authors: XM
authors: Tan
authors: Xiao-Mei
authors: XM
authors: Chen
authors: Chun-Lan
authors: CL
authors: Wang
authors: Xiao-Hua
authors: XH
authors: Jin
authors: Jin-Long
authors: JL
authors: Cui
authors: Juan
authors: J
authors: Chen
authors: Shun-Xing
authors: SX
authors: Guo
authors: Li-Fang
authors: LF
authors: Zhao
mesh_terms: D044822:Biodiversity; D002681:China; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000145:classification / Q000382:microbiology* / Q000502:physiology; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D013559:Symbiosis