title: The impact of recombinant fusion-hydrophobin coated surfaces on E. coli and natural mixed culture biofilm formation.
abstract: The impact of increased surface hydrophobicity on biofilms regarding retardation, repulsion, or attraction was studied with hydrophobin modified glass substrata. Recombinantly produced fungal hydrophobins forming self-assembled monolayers were used as the surface coating. The adsorption dynamics of hydrophobins were analysed with a quartz crystal microbalance which showed the surface coating to be rapid and stable. The change of surface wettability was determined by water contact angle measurements and demonstrated an increase in hydrophobicity in range of 60-62°. The homogeneity of the monolayers was demonstrated by immunofluorescence microscopy. Atomic force microscopy was applied to visualise the uniform texture of the coated materials. The hydrophobin coatings had no impact on different biofilms in terms of spatial distribution, cell numbers, and population composition. In consequence, hydrophobicity might not represent an important parameter for biofilm formation. Nevertheless, recombinant hydrophobins are suitable for large scale surface modification and functionalization with bioactive molecules.
authors: Microbiology of Natural and Technical Interfaces Department, Institute of Functional Interfaces, Karlsruhe Institute of Technology, Karlsruhe, Germany.
authors: Annika
authors: A
authors: Rieder
authors: Tatjana
authors: T
authors: Ladnorg
authors: Christof
authors: C
authors: Wöll
authors: Ursula
authors: U
authors: Obst
authors: Reinhard
authors: R
authors: Fischer
authors: Thomas
authors: T
authors: Schwartz
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D058645:Denaturing Gradient Gel Electrophoresis; D004926:Escherichia coli / Q000187:drug effects*; D005656:Fungal Proteins / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D017155:Wettability / Q000187:drug effects*
chemical_list: D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins