title: [High efficient gene knockout in Verticillium dahliae].
abstract: OBJECTIVE
  We developed an efficient method of gene knockout in Verticillium dahliae, an important soil-borne fungal pathogen that causes cotton vascular wilt diseases.
  
  
  METHODS
  By using fusion PCR, we constructed gene knockout vectors. By using Agrobacterium tumefaciens-mediated transformation and applying a herpes simplex virus thymidine kinase (HSVtk) gene in T-DNA as a conditional lethal gene to counter-select against ectopic transformants, we developed an efficient method to select gene knockout transformants.
  
  
  RESULTS
  Gene knockout frequency for ADE4 and ChsV was 87% and 44% , respectively.
  
  
  CONCLUSIONS
  We developed an efficient tool for gene knockout in Verticillium dahliae, which would help clarify the infection mechanism of this fungal pathogen.
authors: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. tianlicaas@gmail.com
authors: Li
authors: L
authors: Tian
authors: Jieyin
authors: J
authors: Chen
authors: Jiani
authors: J
authors: Wang
authors: Jinlong
authors: J
authors: Wang
authors: Xiaofeng
authors: X
authors: Dai
mesh_terms: D001483:Base Sequence; D055786:Gene Knockout Techniques; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D020172:Verticillium / Q000235:genetics*