title: Identification and in vitro screening of avian yeasts for use as probiotic.
abstract: The objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluate in vitro their potential for probiotic use in animal production.
  
  
  RESULTS
  Nine yeast strains were isolated and presumptively pre-identified by biochemical assays. These isolates were grouped in six different molecular profiles using PCR-fingerprinting technique. Each profile was identified by sequencing of the D1/D2 domains of the large subunit of the 26S rRNA gene. These yeasts were identified as: Trichosporon sp. (LV-2), Wickerhamomyces anomalus (LV-6), Pichia kudriavzevii (LV-8), Kodamaea ohmeri (LV-9) and Trichosporon asahii (LV-10). A pre-screening of the strains for probiotic use was based on their ability to agglutinate pathogenic micro-organisms. These yeast strains were characterized for specific growth rate, duplication time, their cell surface hydrophobicity, medium acidification, resistance to low pH (2.0, 2.5 and 3.0) and concentrations of bile salts (0.3% and 0.6%). The isolate of W. anomalus (LV-6) had the highest agglutinating and adherence capacity, a growth rate of 2.07×10(8) cfu/mL in 24 h at 30 °C, decreasing the medium pH from 6.5 to 5.23, a 25% hydrophobicity and an elevated capacity to grow under stress conditions.
  
  
  CONCLUSIONS
  W. anomalus strain LV-6 showed the best characteristics for use as a probiotic candidate.
  
  
  CONCLUSIONS
  The data from this study helped in choosing a probiotic candidate from yeast to use in broiler production.
authors: Departamento de Fisiología y Bioquímica, Instituto de Ciencia Animal, Habana, Cuba. yaneisyg@ica.co.cu
authors: Yaneisy
authors: Y
authors: García-Hernández
authors: Zoraya
authors: Z
authors: Rodríguez
authors: Luciana R
authors: LR
authors: Brandão
authors: Carlos A
authors: CA
authors: Rosa
authors: Jacques R
authors: JR
authors: Nicoli
authors: Arabel
authors: A
authors: Elías Iglesias
authors: Tania
authors: T
authors: Peréz-Sanchez
authors: Ramón Boucourt
authors: RB
authors: Salabarría
authors: Nabil
authors: N
authors: Halaihel
mesh_terms: D000818:Animals; D001419:Bacteria; D002645:Chickens / Q000276:immunology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D019936:Probiotics / Q000145:classification* / Q000302:isolation & purification*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004271:DNA, Fungal