title: Activity of tumor necrosis factor-α blocked by phytoglycoprotein (38 kDa) at initiation stage in N-nitrosodiethylamine-induced ICR mice.
abstract: Hepatocellular carcinoma is becoming one of the most prominent types of cancer in the world. Recently, from Styrax japonica Siebold et al. Zuccarini (SJSZ), we isolated a glycoprotein which consists of carbohydrate moiety (52.64%) and protein moiety (42.35%). We evaluated whether SJSZ glycoprotein prevents hepatocarcinogenesis induced by N-nitrosodiethylamine (DEN). The purpose of this study was to evaluate the effect of SJSZ glycoprotein in DEN-induced hepatocarcinogenesis in ICR mice. To know chemopreventive effect of SJSZ glycoprotein on hepatocarcinogenesis, ICR mice were intraperitoneally injected with N-nitrosodiethylamine (DEN, 10 mg/kg) for 7 weeks. After sacrifice, we evaluated indicators of liver tissue damage [the activities of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT), and thiobarbituric acid reactive substances (TBARS)], antioxidative enzymes [activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)], and initiating hepatocarcinogenic indicator [heat shock protein (HSP) 27 and 70] and hepatocarcinogenic signals [protein kinase C (PKC), extracellular signal-regulating kinase (ERK) 1/2, nuclear factor (NF)-κB (p50 and p65) and tumor necrosis factor-α (TNF-α)] using biochemical methods, immunoblot analysis, and RT-PCR. The results obtained from this study revealed that SJSZ glycoprotein (10 mg/kg, BW) decreased the levels of LDH, ALT, and TBARS, whereas the activities of SOD, GPx, and CAT increased in the DEN-induced ICR mice. With respect to the hepatocarcinogenic indicator and hepatocarcinogenic signals, HSP27, HSP70, PKC, ERK1/2, NF-κB (p50 and p65), and TNF-α, activity decreased. Hence, SJSZ glycoprotein might prevent expression of HSP27 and HSP70 by DEN.
authors: Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju, South Korea.
authors: Jin
authors: J
authors: Lee
authors: Kye-Taek
authors: KT
authors: Lim
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D004052:Diethylnitrosamine; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006023:Glycoproteins / Q000494:pharmacology*; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D010940:Plant Proteins / Q000494:pharmacology*; D032362:Styrax; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D016588:Anticarcinogenic Agents; D006023:Glycoproteins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D016328:NF-kappa B; D010940:Plant Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; D004052:Diethylnitrosamine; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000410:Alanine Transaminase; D048049:Extracellular Signal-Regulated MAP Kinases