title: Single-molecule analysis of telomerase structure and function.
abstract: The telomerase ribonucleoprotein is a specialized reverse transcriptase required to maintain protective chromosome end-capping structures called telomeres. In most cells, telomerase is not active and the natural shortening of telomeres with each round of DNA replication ultimately triggers cell growth arrest. In contrast, the presence of telomerase confers a high level of renewal capacity upon rapidly dividing cells. Telomerase is aberrantly activated in 90% of human cancers and thus represents an important target for anticancer therapeutics. However, the naturally low abundance of telomerase has hampered efforts to obtain high-resolution models for telomerase structure and function. To circumvent these challenges, single-molecule techniques have recently been employed to investigate telomerase assembly, structure, and catalysis.
authors: Department of Chemistry and Biochemistry, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
authors: Martin
authors: M
authors: Hengesbach
authors: Benjamin M
authors: BM
authors: Akiyama
authors: Michael D
authors: MD
authors: Stone
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics; D021122:Protein Subunits / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019098:Telomerase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016615:Telomere / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D016808:Tetrahymena thermophila
chemical_list: D005456:Fluorescent Dyes; D021122:Protein Subunits; C422952:telomerase RNA; D012313:RNA; C509186:TERT protein, human; D019098:Telomerase