title: Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds.
abstract: In the present study, a porous meniscal-shaped scaffold consisting of polyurethane (PU)-based 1, 4-butanediisocyanate (BDI), which provided a 3-D culture condition for human bone mesenchymal stromal cells (hBMSC) was employed. A bioreactor was utilized to produce perfusion and mechanical stimulations. The viability, proliferation and fibro-cartilaginous differentiation of the hBMSC cultured on the PU-based meniscal scaffold were investigated during the perfusion and mechanical stimulation process. In addition, the mechanical properties of the cell-laden scaffolds were examined as well. Our finding indicated that the perfusion (10 ml/min) and on-off cyclic compressions mechanical stimulation (10% strain, 0.5 Hz, 4 times/day, 2 h/time with 4 h of rest thereafter) maintained the viability and promoted the proliferation of hBMSC over 2 weeks. The on-off cyclic compression caused a 1.85 fold increase in equilibrium modulus. Meanwhile, type I procollagen produced by hBMSC was increased for 3.02-fold after 2 weeks culture. On the other hand, the irrigating medium enhanced the synthesis of type III procollagen for 2.24-fold after 2 weeks. Tensile modulus was elevated for 2.02-fold in perfusion group after 1 week, which was decreased after 2 weeks unexpectedly. Our study suggests that the perfusion and on-off compression are promising to enhance the functional properties of the hBMSC-laden PU-based meniscal scaffold.
authors: Department of Orthopedic Trauma, Hanover Medical School, OE 6230 Carl-Neuberg-Straße 1, D-30625 Hanover, Germany. liuchaoxu.0914@yahoo.com.cn
authors: Chaoxu
authors: C
authors: Liu
authors: Reza
authors: R
authors: Abedian
authors: Roland
authors: R
authors: Meister
authors: Carl
authors: C
authors: Haasper
authors: Christof
authors: C
authors: Hurschler
authors: Christian
authors: C
authors: Krettek
authors: Gabriela
authors: G
authors: von Lewinski
authors: Michael
authors: M
authors: Jagodzinski
mesh_terms: D000328:Adult; D001696:Biomechanical Phenomena; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004867:Equipment Design; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010477:Perfusion / Q000295:instrumentation*; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult
chemical_list: D011140:Polyurethanes