title: [Analyzing hematopoietic development of mouse embryonic AGM region by tissue culture].
abstract: To analyze hematopoietic kinetics of mouse embryonic aorta-gonad-mesonephros (AGM) region, an in vitro tissue culture method was developed in this study, partly based on previous reports. After 2 days of tissue culture, a significant number of erythro myeloid progenitors, as quantitated by colony forming assay was detected in the AGM region. Moreover, the cells from cultured E10.5 AGM region could generate 10.8 ± 3.5 colony-forming unit in spleen (CFU-S) per tissue on average. Transplantation of cultured E10.5-E11.0 AGM cells resulted in efficient (85.7% repopulated) and long-term (> 4 months) reconstitution of lethally irradiated adult recipients with remarkable chimerism [(51.12 ± 21.17)%]. The multilineage contribution of donor cells was validated by significant engraftment of myeloid and/or lymphoid cells in peripheral blood, bone marrow, spleen and thymus of recipients. Taken together, the tissue culture method can enable us to manipulate the AGM region in vitro, fulfilling a systematic evaluation of developmental kinetics of various hematopoietic precursor cells, particularly HSC, in normal and mutant mid-gestation mouse embryos.
authors: Academy of Military Medical Sciences, Beijing, China.
authors: Dong-Bo
authors: DB
authors: Chen
authors: Jiao
authors: J
authors: Gao
authors: Zhuan
authors: Z
authors: Li
authors: Wen-Yan
authors: WY
authors: He
authors: Bing
authors: B
authors: Liu
mesh_terms: D000818:Animals; D005260:Female; D006066:Gonads / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006413:Hematopoietic System / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D046509:Tissue Culture Techniques / Q000379:methods*