title: Changes in the nature of phenolic deposits after re-warming as a result of melatonin pre-sowing treatment of Vigna radiata seeds.
abstract: Changes in phenolics (PhC - phenolic compounds) measured as UV-absorbing compounds (UVAC) and their localization as well as growth, lipid peroxidation (TBARS level) and proline (Pro) level in three-day-old roots of seedlings (T(o) stage) obtained from hydroprimed (H) and hydroprimed with melatonin (H-MEL) seeds after 2 days of chilling (5°C) and 2 days of re-warming were examined. H and H-MEL resulted in inhibition of root growth under optimal conditions, but after re-warming, a positive effect of MEL was noted. The results also showed a positive MEL impact on TBARS level already after chilling and especially after re-warming. Exposure of Vigna radiata seedlings to chilling caused a significant increase in Pro level, especially in H-MEL roots, but after re-warming it drastically decreased. Under chilling stress, accumulation of UVAC also decreased. However, after re-warming it returned to the level observed in the roots grown constantly at 25°C. Even if after re-warming of V. radiata seedlings only slight accumulation of total PhC was observed, phenolic deposits accumulating in the vacuoles of H-MEL roots were completely different from those in the vacuoles of the control and H roots. H-MEL application to the seeds resulted in a significant increase in small granular composite materials, while in the control and H roots, large oval deposits prevailed. Taken together, it is probable that all of these differences were connected with positive effects of MEL on chilled V. radiata seedlings after re-warming.
authors: Department of Ecophysiology and Plant Development, University of Lodz, Faculty of Biology and Plant Environmental Protection, Banacha 12/16, 90-237 Lodz, Poland. keysi@biol.uni.lodz.pl
authors: Katarzyna
authors: K
authors: Szafrańska
authors: Sława
authors: S
authors: Glińska
authors: Krystyna Maria
authors: KM
authors: Janas
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D003080:Cold Temperature / Q000009:adverse effects; D007887:Fabaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects; D008550:Melatonin / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D010636:Phenols / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012639:Seeds / Q000187:drug effects* / Q000378:metabolism; D013312:Stress, Physiological; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010636:Phenols; D010860:Pigments, Biological; D017392:Thiobarbituric Acid Reactive Substances; D011392:Proline; D008550:Melatonin