title: A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune.
abstract: A 20-kDa ribonuclease (RNase) was purified from fresh fruiting bodies of cultured Schizophyllum commune mushrooms. The RNase was not adsorbed on Affi-gel blue gel but adsorbed on DEAE-cellulose and CM-cellulose. It exhibited maximal RNase activity at pH 6.0 and 70°C. It demonstrated the highest ribonucleolytic activity toward poly (U) (379.5 μ/mg), the second highest activity toward poly (C) (244.7 μ/mg), less activity toward poly (A) (167.4 μ/mg), and much weaker activity toward poly (G) (114.5 μ/mg). The RNase inhibited HIV-1 reverse transcriptase with an IC(50) of 65 μM. No effect on [(3)H-methyl]-thymidine uptake by lymphoma MBL2 cells and leukemia L1210 cells was observed at 100 μM concentration of the RNase. A comparison of RNases from S. commune and Volvariella volvacea revealed that they demonstrated some similarities in N-terminal amino acid sequence, optimum pH and polyhomoribonucleotide specificity. However, some differences in chromatographic behavior and molecular mass were observed.
authors: Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming, 650223, P. R. China.
authors: Yong-Chang
authors: YC
authors: Zhao
authors: Guo-Qing
authors: GQ
authors: Zhang
authors: Tzi-Bun
authors: TB
authors: Ng
authors: He-Xiang
authors: HX
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D004795:Enzyme Stability; D048690:Fruiting Bodies, Fungal / Q000201:enzymology; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D011068:Poly G / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D012567:Schizophyllum / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D011061:Poly A; D011068:Poly G; D011072:Poly U; D011066:Poly C; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases