title: Presence, molecular characteristics and geosmin producing ability of actinomycetes isolated from South Korean terrestrial and aquatic environments.
abstract: The unpleasant odor of drinking water is one of the major problems in many water utilities in the world. Actinomycetes have long been associated with odorous compounds. Considering the paucity of research on Actinomycetes producing odorous compounds in South Korea, presence of Actinomycetes, their molecular characteristics and ability to produce odorous compounds were investigated in this study. Findings confirmed the presence of Actinomycetes in surface soil, sediment, and water samples from four sites: two artificial lakes [Paldang and Cheongpyeong (CP)], and two streams [Gyeongan (GA) and Yangpyeong]. Surface soil and sediment from CP area had the greatest concentration of Actinomycetes (8.2 x 10(7) and 6.8 x 10(6) colony forming units (CFUs)/gram, dry weight, respectively). When water samples are considered, samples from GA had the highest concentration (1.9 x 10(2) CFU/mL). 16S rRNA sequencing and molecular phylogenetic analysis showed that Streptomyces was the dominant genus (64.1%). In addition, the isolated Actinomycetes synthesized 5.4 ng/L geosmin as demonstrated by thermal desorption unit-gas chromatograph/mass spectrometry analysis.
authors: Water Analysis and Research Center, K-water, 560 Sintanjin-ro, Daedeok-gu, Daejeon 306-711, Republic of Korea.
authors: Gyu-Cheol
authors: GC
authors: Lee
authors: Yun S
authors: YS
authors: Kim
authors: Min-Jeong
authors: MJ
authors: Kim
authors: Sung-Ae
authors: SA
authors: Oh
authors: Ilhwan
authors: I
authors: Choi
authors: Jaewon
authors: J
authors: Choi
authors: Jong-Geun
authors: JG
authors: Park
authors: Chom-Kyu
authors: CK
authors: Chong
authors: Yong-Yeon
authors: YY
authors: Kim
authors: Kyeunghee
authors: K
authors: Lee
authors: Chan Hee
authors: CH
authors: Lee
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification*; D019015:Geologic Sediments; D010802:Phylogeny; D056910:Republic of Korea; D012988:Soil Microbiology; D014871:Water Microbiology; D014881:Water Supply