title: RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses.
abstract: Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genome-wide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release, were identified. Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolutionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.
authors: School of Veterinary Medicine and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583, USA.
authors: Debasis
authors: D
authors: Panda
authors: Anshuman
authors: A
authors: Das
authors: Phat X
authors: PX
authors: Dinh
authors: Sakthivel
authors: S
authors: Subramaniam
authors: Debasis
authors: D
authors: Nayak
authors: Nicholas J
authors: NJ
authors: Barrows
authors: James L
authors: JL
authors: Pearson
authors: Jesse
authors: J
authors: Thompson
authors: David L
authors: DL
authors: Kelly
authors: Istvan
authors: I
authors: Ladunga
authors: Asit K
authors: AK
authors: Pattnaik
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D058605:Host-Derived Cellular Factors / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics / Q000502:physiology; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000502:physiology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055571:Secretory Pathway / Q000235:genetics*; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000502:physiology*; D016662:Virus Integration / Q000235:genetics*
chemical_list: D058605:Host-Derived Cellular Factors; D034741:RNA, Small Interfering