title: [Optimization of microwave-assisted extraction with RSM and HPLC determination for three iridoid glycosides in Cornus officinalis].
abstract: OBJECTIVE
  To establish a method of microwave-assisted extraction and high performance liquid chromatographic (HPLC) for simultaneous determination of three iridoid glycosides including loganin, sweroside and cornuside in Cornus officinalis.
  
  
  METHODS
  The extraction conditions of microwave power,ethanol concentration, liquid to sample ratio were optimized with a response surface methodology (RSM); Three constituents were separated on an Agilent TC-C18 column by gradient elution using acetonitrile and water as the mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 240 nm.
  
  
  RESULTS
  The optimal conditions of microwave extraction were as follows: microwave power 400 W, ethanol concentration 72%, liquid to sample ratio 15 mL/g, the extraction time 10 min, the extraction times 2; The HPLC peak areas of all the constituents showed good linearity (r>0.9994) in the range of the tested concentration,the average recoveries of the method were 98.68%, 98.24% and 98.29%, respectively.
  
  
  CONCLUSIONS
  The established method of microwave-assisted extraction and HPLC simultaneously determination has the advantages of convenient, precision and reliability. It can be used in simultaneous determination of three iridoid glycosides in Cornus officinalis.
authors: Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry of Ministry of Education, School of Chemistry and Material, Shaanxi Normal University, Xi'an 710062, China. hjn2008@stu.snnu.edu.cn
authors: Juan-ni
authors: JN
authors: He
authors: Xiao
authors: X
authors: Liu
authors: Zhao-li
authors: ZL
authors: Mou
authors: Han-ying
authors: HY
authors: Zhan
authors: Zhi-qi
authors: ZQ
authors: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029768:Cornus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D057888:Iridoid Glucosides / Q000032:analysis* / Q000302:isolation & purification; D039823:Iridoids / Q000032:analysis* / Q000302:isolation & purification; D008872:Microwaves; D011714:Pyrans / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; D012997:Solvents; C080726:cornuside; C059516:loganin; C049412:sweroside