title: Cloning and characterization of a phosphate transporter gene in Dunaliella salina.
abstract: The full-length cDNA of a Na(+) -dependent Pi transport gene (DsSPT1) in Dunaliella salina was cloned by 3' and 5' Rapid Amplification of cDNA Ends (RACE), with an open reading frame (ORF) encoding 716 predicted amino acids, which exhibited 60.5% identity to that of Na(+) -dependent Pi transport 1 (DvSPT1) from Dunaliella viridis. Hydrophobicity and secondary structure prediction revealed 11 conserved transmembrane domains similar to those found in DvSPT1 from D. viridis and PHO89 from Saccharomyces cerevisiae. The result of real-time quantitative PCR showed that expression level of DsSPT1 was enhanced at first and reached its peak at 90 min after salt stress; however, D. salina cells rapidly absorbed extracellular inorganic phosphorus which was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) during the first 5 min under salt stress. It suggested that D. salina on the absorption of inorganic phosphorus was regulated at DsSPTI posttranslational level.
authors: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Shao-He
authors: SH
authors: Li
authors: Bing-Bing
authors: BB
authors: Xia
authors: Chi
authors: C
authors: Zhang
authors: Jiao
authors: J
authors: Cao
authors: Lin-Han
authors: LH
authors: Bai
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling; D057927:Hydrophobic and Hydrophilic Interactions; D016366:Open Reading Frames; D028061:Phosphate Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D013054:Spectrophotometry, Atomic; D016823:Volvocida / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D028061:Phosphate Transport Proteins; D010758:Phosphorus