title: The human immunodeficiency virus-1 genotype diversity and drug resistance mutations profile of volunteer blood donors from Chinese blood centers.
abstract: BACKGROUND
  The global human immunodeficiency virus (HIV)-1 epidemic is becoming increasingly diverse and complex. Molecular epidemiologic characteristics were studied for HIV-1-infected blood donors from five Chinese regions to determine genotype diversity and drug resistance mutations (DRMs) profile.
  
  
  METHODS
  HIV-1 confirmed-reactive serum samples were collected from 172 blood donors from five blood centers during 2007 to 2010. HIV-1 Pol including whole protease and partial reverse transcriptase genes was amplified, sequenced, and analyzed for the subtype determination and drug resistance profile description.
  
  
  RESULTS
  A total of 113 amplified sequences including 82 from Kunming blood center and 31 from four other blood centers had the following genotype characteristics: G (0.9%), B (2.7%), circulating recombinant form (CRF) 01_AE (32.7%), CRF07_BC (22.1%), and CRF08_BC (41.6%). Female donors represent 45.1% of all cases and 63.9% cases with DRMs. The prevalence of samples with potential low or higher resistance among Chinese blood donors is 4.4%.
  
  
  CONCLUSIONS
  HIV-1 infection in Chinese blood donors is genetically diverse and the subtype distribution reflects that from the high-risk populations. Our results support continuous molecular epidemiologic surveillance for HIV-1 in blood donors as a part of a comprehensive HIV control program.
authors: Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, Sichuan, China.
authors: Peibin
authors: P
authors: Zeng
authors: Jingxing
authors: J
authors: Wang
authors: Yi
authors: Y
authors: Huang
authors: Xiaoming
authors: X
authors: Guo
authors: Julin
authors: J
authors: Li
authors: Guoxin
authors: G
authors: Wen
authors: Tonghan
authors: T
authors: Yang
authors: Zhongqiao
authors: Z
authors: Yun
authors: Miao
authors: M
authors: He
authors: Yu
authors: Y
authors: Liu
authors: Yuzhe
authors: Y
authors: Yuan
authors: Jane
authors: J
authors: Schulmann
authors: Simone
authors: S
authors: Glynn
authors: Paul
authors: P
authors: Ness
authors: J Brooks
authors: JB
authors: Jackson
authors: Hua
authors: H
authors: Shan
mesh_terms: D000328:Adult; D000368:Aged; D001782:Blood Donors; D002681:China; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015497:HIV-1 / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation