title: Evaluation of the contribution of skatole, indole, androstenone and androstenols to boar-taint in back fat of pigs by HPLC and capillary gas chromatography (CGC).
abstract: Fifteen samples of fat with boar taint were selected by means of a trained panel-test, from 200 carcasses of entire male pigs, and they were analyzed to determine skatole, indole, 5α-Androst-16-en-3-one (5α-An), 5α-Androst-16-en-3α-ol (5αAn3α) and 5α-Androst-16-en-3β-ol (5αAn3β). The extraction was carried out with methanol, the extract was placed at -20°C during 10 min to eliminate the fat and after evaporation of methanol a Florisil clean up was applied to obtain skatole and indole in a separate fraction from 5αAn and androstenols. Skatole and indole were determined by HPLC in normal phase with a Spherisorb-NH(2) column and 5αAn and androstenols were analyzed by capillary gas chromatography (CGC). Selective procedures were used for detection: ratio at 220 nm and 280 nm (skatole/indole) and CGC coupled to a mass spectrometer working in the SIM (selected ion monitoring) mode at m/z 272 (o5αAn) and m/z 274 (androstenols). The results obtained showed that skatole and 5αAn concentrations exceeded that typical thresholds values in more than 50% of samples analyzed, suggesting a similar influence on boar taint. 5αAn3β showed higher concentrations than 5αAn3α, but lower than 5αAn, exceeding only 1·00 μg/g in three samples.
authors: (IRTA), Centre Tecnologia de la Carn, Generalitat de Catalunya, Granja Camps i Armet, 17121 Monells, Girona, Spain.
authors: J A
authors: JA
authors: García-Regueiro
authors: I
authors: I
authors: Diaz