title: Degradation of endocrine disrupting chemicals by genetic transformants with two lignin degrading enzymes in Phlebia tremellosa.
abstract: A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions. An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain.
authors: Molecular Microbiology Lab, Department of Biochemistry, Kangwon National University, Chunchon, 200-701, Republic of Korea.
authors: Hyunwoo
authors: H
authors: Kum
authors: Sungsuk
authors: S
authors: Lee
authors: Sunhwa
authors: S
authors: Ryu
authors: Hyoung T
authors: HT
authors: Choi
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism*; D001559:Benzhydryl Compounds; D001711:Biotransformation; D003001:Cloning, Molecular; D052244:Endocrine Disruptors / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D030781:Organisms, Genetically Modified; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D014170:Transformation, Genetic; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D042845:Laccase; D008031:Lignin; D010544:Peroxidases; D010636:Phenols; C025256:nonylphenol; C051129:manganese peroxidase; C006780:bisphenol A; D000098826:Bisphenol A Compounds