title: 1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a superoxide generation inhibitor from Perilla frutescens var. crispa.
abstract: Using a superoxide (O(2)(-)) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O(2)(-) generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O(2)(-) level in a dose-dependent manner with an IC(50) value of 21 µM, comparable to those of rosmarinic acid (RoA, IC(50) = 29 µM) and caffeic acid (CA, IC(50) = 30 µM). While RoA and CA also dose-dependently inhibited O(2)(-) generation in a xanthine-xanthine oxidase system, DLGG had no effect in the same system. Thus DLGG appeared to decrease the O(2)(-) level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O(2)(-) scavengers.
authors: Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Eiheji-cho, Fukui, Japan. mastak@fpu.ac.jp
authors: Masakazu
authors: M
authors: Takahashi
authors: Yuta
authors: Y
authors: Sugiyama
authors: Kyuichi
authors: K
authors: Kawabata
authors: Yu
authors: Y
authors: Takahashi
authors: Kazuhiro
authors: K
authors: Irie
authors: Akira
authors: A
authors: Murakami
authors: Yoshito
authors: Y
authors: Kubo
authors: Kyoichi
authors: K
authors: Kobayashi
authors: Hajime
authors: H
authors: Ohigashi
mesh_terms: D002109:Caffeic Acids / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism; D053630:Depsides / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013481:Superoxides / Q000037:antagonists & inhibitors*; D014969:Xanthine Oxidase / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D016166:Free Radical Scavengers; D005960:Glucosides; D005990:Glycerol; D010936:Plant Extracts; D013481:Superoxides; D014969:Xanthine Oxidase; D000097123:Rosmarinic Acid; C419082:1,2-dilinolenoyl-3-galactopyranosylglycerol; C040048:caffeic acid