title: Evaluation of cytochrome P450 activities in human hepatocytes in vitro.
abstract: Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy in combination with mass spectrometry is shown to be a robust, fast, and sensitive procedure for P450 activity assessment. This procedure allows a drastic reduction of the number of cells required in the assay and sample analysis time and increases throughput and reproducibility. Major applications of the probe cocktail strategy are P450 phenotyping of hepatocytes and induction studies.
authors: Unidad de Hepatología Experimental, Centro de Investigación. Hospital La Fe, Valencia, Spain. gomez_mjo@gva.es
authors: María José
authors: MJ
authors: Gómez-Lechón
authors: Agustín
authors: A
authors: Lahoz
authors: José V
authors: JV
authors: Castell
authors: María Teresa
authors: MT
authors: Donato
mesh_terms: D003470:Culture Media / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D022781:Hepatocytes / Q000201:enzymology*; D006801:Humans; D061251:Primary Cell Culture / Q000379:methods
chemical_list: D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System