title: Alpha-tocopherol counteracts the cytotoxicity induced by ochratoxin a in primary porcine fibroblasts.
abstract: The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.
authors: Università degli Studi di Milano, Department of Veterinary Science and Technology for Food Safety, Via Trentacoste 2, I-20134 Milan, Italy. eleonora.fusi@unimi.it
authors: Eleonora
authors: E
authors: Fusi
authors: Raffaella
authors: R
authors: Rebucci
authors: Chiara
authors: C
authors: Pecorini
authors: Anna
authors: A
authors: Campagnoli
authors: Luciano
authors: L
authors: Pinotti
authors: Francesca
authors: F
authors: Saccone
authors: Federica
authors: F
authors: Cheli
authors: Stig
authors: S
authors: Purup
authors: Kristen
authors: K
authors: Sejrsen
authors: Antonella
authors: A
authors: Baldi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007928:Lethal Dose 50; D009793:Ochratoxins / Q000633:toxicity*; D013552:Swine; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D009793:Ochratoxins; C025589:ochratoxin A; D007770:L-Lactate Dehydrogenase; D024502:alpha-Tocopherol