title: Towards the role of metal ions in the structural variability of proteins: CdII speciation of a metal ion binding loop motif.
abstract: A de novo designed dodecapeptide (HS), inspired by the metal binding loops of metal-responsive transcriptional activators, was synthesized. The aim was to create a model system for structurally promiscuous and intrinsically unstructured proteins, and explore the effect of metal ions on their structure and dynamics. The interaction with Cd(II) was investigated by UV, synchrotron radiation CD, (1)H NMR, and perturbed angular correlation (PAC) of γ-rays spectroscopy, pH-potentiometry, and molecular modelling. The peptide mainly displays characteristics of random coil in the CD spectra, and the molecular dynamics simulations demonstrate that it is unstructured with transient and varying helical content. The spectroscopic studies revealed the formation of loop structures with the coordination of the two Cys-thiolates close to each end of the HS peptide, in the presence of one equivalent of Cd(II) per ligand. The imidazole moiety from histidine is also bound to Cd(II) at neutral pH and above. In the presence of 0.5 equivalent of Cd(II) per HS metal bridged structures with e.g. CdS(2)N(2) and possibly CdS(4) coordination geometries are formed above pH ~6. In an equilibrium of several co-existing species the peptide is exchanging between a number of structures also in its metal ion bound state(s), as indicated by NMR and PAC data.
authors: Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary. jancso@chem.u-szeged.hu
authors: Attila
authors: A
authors: Jancsó
authors: Dániel
authors: D
authors: Szunyogh
authors: Flemming H
authors: FH
authors: Larsen
authors: Peter W
authors: PW
authors: Thulstrup
authors: Niels Johan
authors: NJ
authors: Christensen
authors: Béla
authors: B
authors: Gyurcsik
authors: Lars
authors: L
authors: Hemmingsen
mesh_terms: D000595:Amino Acid Sequence; D002104:Cadmium / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D003545:Cysteine / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011199:Potentiometry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D010455:Peptides; D013438:Sulfhydryl Compounds; D002104:Cadmium; D003545:Cysteine