title: Transit time affects the community stability of Lactobacillus and Bifidobacterium species in an in vitro model of human colonic microbiotia.
abstract: Retention time, which is analogous to transit time, is an index for bacterial stability in the intestine. Its consideration is of particular importance to optimize the delivery of probiotic bacteria in order to improve treatment efficacy. This study aims to investigate the effect of retention time on Lactobacilli and Bifidobacteria stability using an established in vitro human colon model. Three retention times were used: 72, 96, and 144 h. The effect of retention time on cell viability of different bacterial populations was analyzed with bacterial plate counts and PCR. The proportions of intestinal Bifidobacteria, Lactobacilli, Enterococci, Staphylococci and Clostridia populations, analyzed by plate counts, were found to be the same as that in human colonic microbiota. Retention time in the human colon affected the stability of Lactobacilli and Bifidobacteria communities, with maximum stability observed at 144 h. Therefore, retention time is an important parameter that influences bacterial stability in the colonic microbiota. Future clinical studies on probiotic bacteria formulations should take into consideration gastrointestinal transit parameters to improve treatment efficacy.
authors: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors: Laetitia
authors: L
authors: Rodes
authors: Arghya
authors: A
authors: Paul
authors: Michael
authors: M
authors: Coussa-Charley
authors: Hani
authors: H
authors: Al-Salami
authors: Catherine
authors: C
authors: Tomaro-Duchesneau
authors: Marc
authors: M
authors: Fakhoury
authors: Satya
authors: S
authors: Prakash
mesh_terms: D001644:Bifidobacterium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D003106:Colon / Q000382:microbiology* / Q000502:physiology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D005772:Gastrointestinal Transit; D006801:Humans; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D054892:Metagenome; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial