title: Butamben derivatives enhance BMP-2-stimulated commitment of C2C12 cells into osteoblasts with induction of voltage-gated potassium channel expression.
abstract: As the primary step for 'drug repositioning', we evaluated the effect of 2000 drugs and drug candidates on the commitment of bi-potential mesenchymal precursor C2C12 cells into osteoblasts in the presence of bone morphogenetic protein (BMP)-2 and found that butamben enhanced BMP-2-stimulated induction of alkaline phosphatase, a biomarker of osteoblastogenesis. Investigating the underlying mechanism of its anabolic actions, we found anabolic action of its derivative (compound 4) relies on BMP-2 signaling and mRNA induction of BMPs and voltage-gated potassium channels.
authors: Laboratory of Chemical Genomics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, PO Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea.
authors: Hye Joo
authors: HJ
authors: Kim
authors: Mikyung
authors: M
authors: Park
authors: Young-Min
authors: YM
authors: Han
authors: Byoung-Mog
authors: BM
authors: Kwon
authors: Seong Hwan
authors: SH
authors: Kim
mesh_terms: D010129:4-Aminobenzoic Acid / Q000737:chemistry / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001566:Benzocaine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D062366:para-Aminobenzoates
chemical_list: D055396:Bone Morphogenetic Protein 2; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D062366:para-Aminobenzoates; D000469:Alkaline Phosphatase; C004605:butamben; D010129:4-Aminobenzoic Acid; D001566:Benzocaine