title: Identification of Mycobacterium bovis among mycobacterial isolates from human clinical specimens at a university hospital in Rio de Janeiro, Brazil.
abstract: In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510), and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected.
authors: Universidade Federal Fluminense, Niterói, RJ, Brasil. lucianafonsecas@gmail.com
authors: Luciana Fonseca
authors: LF
authors: Sobral
authors: Rafael Silva
authors: RS
authors: Duarte
authors: Gisele Betzler de Oliveira
authors: GB
authors: Vieira
authors: Marlei Gomes da
authors: MG
authors: Silva
authors: Neio
authors: N
authors: Boechat
authors: Leila de Souza
authors: Lde S
authors: Fonseca
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D001938:Brazil / Q000453:epidemiology; D003470:Culture Media / Q000737:chemistry; D006785:Hospitals, University; D006801:Humans; D009163:Mycobacterium bovis / Q000254:growth & development*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000302:isolation & purification*; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D003470:Culture Media