title: Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium.
abstract: Surface modification of titanium (Ti) using biomolecules has attracted much attention recently. In this study, a new strategy has been employed to construct a stable and bioactive coating on Ti. To this end, a derivative of hyaluronic acid (HA), i.e. HA-GRGDSPC-(SH), was synthesized. The disulfide-crosslinked Arg-Gly-Asp (RGD)-containing collagen/hyaluronic acid polyelectrolyte membrane (PEM) coating was then fabricated on Ti through the alternate deposition of collagen and HA-GRGDSPC-(SH) with five assembly cycles and subsequent crosslinking via converting free sulphydryl groups into disulfide linkages (RGD-CHC-Ti group). The assembly processes for PEM coating and the physicochemical properties of the coating were carefully characterized. The stability of PEM coating in phosphate-buffered saline solution could be adjusted by the crosslinking degree, while its degradation behaviors in the presence of glutathione were glutathione concentration dependent. The adhesion and proliferation of MC3T3-E1 cells were significantly enhanced in the RGD-CHC-Ti group. Up-regulated bone specific genes, enhanced alkaline phosphatase activity and osteocalcin production, the increased areas of mineralization were also observed in the RGD-CHC-Ti group. These results indicate that the strategy employed herein may function as an effective way to construct stable, RGD-containing bioactive coatings on Ti.
authors: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, People's Republic of China.
authors: Ying
authors: Y
authors: Huang
authors: Qiaojie
authors: Q
authors: Luo
authors: Xiaodong
authors: X
authors: Li
authors: Feng
authors: F
authors: Zhang
authors: Shifang
authors: S
authors: Zhao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004573:Electrolytes / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry; D008422:Materials Testing / Q000379:methods*; D008567:Membranes, Artificial; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D015675:Osteocalcin / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D004573:Electrolytes; D008567:Membranes, Artificial; D009842:Oligopeptides; D010455:Peptides; D012333:RNA, Messenger; D015675:Osteocalcin; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid; D003094:Collagen; D014025:Titanium; D000469:Alkaline Phosphatase