title: Quantitative matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analysis of synthetic polymers and peptides.
abstract: Matrix-assisted laser desorption ionization (MALDI) is a very powerful and widely used mass spectrometric technique to ionize high molecular weight compounds. The most commonly used dried droplet (DD) technique can lead to a concentration distribution of the analyte on the target and is therefore often not suitable for reproducible analyses. We developed a new solvent-free deposition technique, called compressed sample (CS), to prevent the distribution of the analytes caused by the crystallization of the compounds. The CS technique presented in this work allows the quantitative analysis of synthetic polymers such as derivatized maltosides with correlation coefficients of 0.999 and peptides up to 3500 Da with correlation coefficients of at least 0.982 without the use of stable-isotope-labeled standards.
authors: Institute for Pure and Applied Mass Spectrometry, University of Wuppertal, Wuppertal, Germany.
authors: Lukas
authors: L
authors: Hyzak
authors: Rebecca
authors: R
authors: Moos
authors: Friederike
authors: F
authors: von Rath
authors: Volker
authors: V
authors: Wulf
authors: Michaela
authors: M
authors: Wirtz
authors: David
authors: D
authors: Melchior
authors: Hans-Willi
authors: HW
authors: Kling
authors: Michael
authors: M
authors: Köhler
authors: Siegmar
authors: S
authors: Gäb
authors: Oliver J
authors: OJ
authors: Schmitz
mesh_terms: D010455:Peptides / Q000032:analysis*; D011108:Polymers / Q000032:analysis*; D011887:Raffinose / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013193:Stainless Steel
chemical_list: D010455:Peptides; D011108:Polymers; D013193:Stainless Steel; D011887:Raffinose