title: Effect of phospholipid deposits on adhesion of bacteria to contact lenses.
abstract: OBJECTIVE
  Protein and lipid deposits on contact lenses may contribute to clinical complications. This study examined the effect of phospholipids on the adhesion of bacteria to contact lenses.
  
  
  METHODS
  Worn balafilcon A (n = 11) and senofilcon A (n = 11) were collected after daily wear and phospholipids were extracted in chloroform:methanol. The amount of phospholipid was measured by electrospray ionization mass spectrometry. Unworn lenses soaked in phospholipids were exposed to Pseudomonas aeruginosa and Staphylococcus aureus. After 18 h incubation, the numbers of P. aeruginosa or S. aureus that adhered to the lenses were measured. Phospholipid was tested for possible effects on bacterial growth.
  
  
  RESULTS
  A broad range of sphingomyelins (SM) and phosphatidylcholines (PC) were detected from both types of worn lenses. SM (16:0) (m/z 703) and PC (34:2) (m/z 758) were the major phospholipids detected in the lens extracts. Phospholipids did not alter the adhesion of any strain of P. aeruginosa or S. aureus (p > 0.05). Phospholipids (0.1 mg/mL) showed no effect on the growth of P. aeruginosa 6294 or S. aureus 031.
  
  
  CONCLUSIONS
  Phospholipids adsorb/absorb to contact lenses during wear, however, the major types of phospholipids adsorbed to lenses do not alter bacterial adhesion or growth.
authors: Brien Holden Vision Institute, Sydney, New South Wales, Australia. n.babaei@brienholdenvision.org
authors: Negar
authors: N
authors: Babaei Omali
authors: Nicholas
authors: N
authors: Proschogo
authors: Hua
authors: H
authors: Zhu
authors: Zhenjun
authors: Z
authors: Zhao
authors: Jennie
authors: J
authors: Diec
authors: Roya
authors: R
authors: Borazjani
authors: Mark D P
authors: MD
authors: Willcox
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D003262:Contact Lenses, Extended-Wear / Q000382:microbiology*; D006801:Humans; D020100:Hydrogels; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development*; D012828:Silicones; D021241:Spectrometry, Mass, Electrospray Ionization; D013109:Sphingomyelins / Q000032:analysis; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*; D013499:Surface Properties
chemical_list: D020100:Hydrogels; D010713:Phosphatidylcholines; D010743:Phospholipids; D012828:Silicones; D013109:Sphingomyelins; C523195:balafilcon A; C523196:senofilcon A