title: Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test.
abstract: The genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.
authors: Laboratoire d'Ecologie et Environnement-Unité Associée au CNRST, URAC 32, Unité Associée au CNERS, Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, BP 2390, Marrakech, Maroc.
authors: A
authors: A
authors: Khadra
authors: E
authors: E
authors: Pinelli
authors: M Z
authors: MZ
authors: Lacroix
authors: A
authors: A
authors: Bousquet-Melou
authors: H
authors: H
authors: Hamdi
authors: G
authors: G
authors: Merlina
authors: M
authors: M
authors: Guiresse
authors: M
authors: M
authors: Hafidi
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity; D004249:DNA Damage; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000633:toxicity*; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D015363:Quinolones / Q000633:toxicity*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000633:toxicity*; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D009153:Mutagens; D015363:Quinolones; D012987:Soil; D012989:Soil Pollutants; D000077422:Enrofloxacin