title: [Cloning and sequencing full-length genome of H5N1 avian influenza A virus and molecular evolution analysis of HA gene].
abstract: OBJECTIVE
  To clone and sequence full-length genome of the avian influenza A/Chicken/Hubei/489/ 2004(H5N1) virus, in order to analyze genetic mutation patterns of HA gene and polygenetic relationship between A/Chicken/Hubei/489/2004 (H5N1) virus and other strains since 1996.
  
  
  METHODS
  8 genes of the avian influenza A/Chicken/Hubei/489/2004 (H5N1) virus were amplified and cloned, and then genetic mutation analysis and phylogenetic trees were made by bioinformatics software.
  
  
  RESULTS
  Full-length genome of the avian influenza A/Chicken/Hubei/489/2004 (H5N1) virus were cloned into the vector of PMD18-T. Genetic evolution analysis showed that there is a specific cleavage site of "PQRERRRKKR", which was proved be related with virulence. In addition, molecular phylogenetic trees of HA gene revealed that A/Chicken/Hubei/ 489/2004 virus were closely related to H5N1 viruses of 2000-2006 isolated in Hong Kong and in Southeast Asia.
  
  
  CONCLUSIONS
  The influenza A/Chicken/Hubei/489/2004 (H5N1) virus was closest genetic relatives to the influenza A/Chicken/HongKong/YU777/2002 (H5N1) virus, and it was most possible that the avian influenza outbreak was caused by the 2002 lineage of Hong Kong.
authors: Hubei Provinical Center for Disease Control and Prevention, Wuhan 430079, China. xjqq@tom.com
authors: Junqiang
authors: J
authors: Xu
authors: Shuwen
authors: S
authors: Wu
authors: Faxian
authors: F
authors: Zhan
authors: Yongzhong
authors: Y
authors: Jiang
authors: Xixiang
authors: X
authors: Huo
authors: Mingxia
authors: M
authors: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002681:China; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D016679:Genome, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus