title: Subcellular evidence for the involvement of peroxisomes in plant isoprenoid biosynthesis.
abstract: The role of peroxisomes in isoprenoid metabolism, especially in plants, has been questioned in several reports. A recent study of Sapir-Mir et al. revealed that the two isoforms of isopentenyl diphosphate (IPP) isomerase, catalyzing the isomerisation of IPP to dimethylallyl diphosphate (DMAPP) are found in the peroxisome. In this addendum, we provide additional data describing the peroxisomal localization of 5-phosphomevalonate kinase and mevalonate 5-diphosphate decarboxylase, the last two enzymes of the mevalonic acid pathway leading to IPP. This finding was reinforced in our latest report showing that a short isoform of farnesyl diphosphate, using IPP and DMAPP as substrates, is also targeted to the organelle. Therefore, the classical sequestration of isoprenoid biosynthesis between plastids and cytosol/ER can be revisited by including the peroxisome as an additional isoprenoid biosynthetic compartment within plant cells.
authors.affiliation: Université François-Rabelais de Tours, EA2106, Biomolécules et Biotechnologies Végétales, Tours, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Benoit
authors.initials: B
authors.lastname: St-Pierre
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Simkin
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D019748:Carbon-Carbon Double Bond Isomerases / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D030008:Catharanthus / Q000201:enzymology*; D045782:Hemiterpenes / Q000096:biosynthesis; D009943:Organophosphorus Compounds; D020675:Peroxisomes / Q000201:enzymology*; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism*
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D010940:Plant Proteins; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; D013729:Terpenes; C004809:isopentenyl pyrophosphate; C004808:farnesyl pyrophosphate; D017856:Phosphotransferases (Phosphate Group Acceptor); C022371:phosphomevalonate kinase; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase
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