title: Cyanine fluorophore derivatives with enhanced photostability.
abstract: Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
authors.affiliation: Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York, USA.
authors.forename: Roger B
authors.initials: RB
authors.lastname: Altman
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Terry
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qinsi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Peter
authors.initials: P
authors.lastname: Geggier
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Kolster
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Javitch
authors.forename: J David
authors.initials: JD
authors.lastname: Warren
authors.forename: Scott C
authors.initials: SC
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000737:chemistry; D016466:CHO Cells; D002232:Carbocyanines / Q000737:chemistry*; D002839:Chromans / Q000737:chemistry; D006224:Cricetinae; D034242:Cyclooctanes / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004355:Drug Stability; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D038761:Photobleaching / Q000187:drug effects; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D026082:Singlet Oxygen / Q000032:analysis
chemical_list: D001592:Benzyl Alcohols; D002232:Carbocyanines; D002839:Chromans; D034242:Cyclooctanes; D005456:Fluorescent Dyes; D017448:Receptors, Dopamine D2; C085321:cyanine dye 5; D026082:Singlet Oxygen; C046885:4-nitrobenzyl alcohol; D004247:DNA; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
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