title: Effect of modified glucose catabolism on xanthan production in Xanthomonas oryzae pv. oryzae.
abstract: In this study, the glucose 6-phosphate dehydrogenase gene (XOO2314) was inactivated in order to modulate the intracellular glucose 6-phosphate, and its effects on xanthan production in a wild-type strain of Xanthomonas oryzae were evaluated. The intracellular glucose 6-phosphate was increased from 17.6 to 99.4 μmol g⁻¹ (dry cell weight) in the gene-disrupted mutant strain. The concomitant increase in the glucose 6-phosphate was accompanied by an increase in xanthan production of up to 2.23 g l⁻¹ (culture medium). However, in defined medium supplemented with 0.4% glucose, the growth rate of the mutant strain was reduced to 52.9% of the wild-type level. Subsequently, when a family B ATP-dependent phosphofructokinase from Escherichia coli was overexpressed in the mutant strain, the growth rate was increased to 142.9%, whereas the yields of xanthan per mole of glucose remained approximately the same.
authors.affiliation: Department of Pharmaceutical Engineering, College of Health Science, Sangji University, Sangjidae-gil 83, Woosan-dong, Wonju-si, Gangwon-do 220-702, Korea.
authors.forename: Se-Gul
authors.initials: SG
authors.lastname: Jang
authors.forename: Byoung-Moo
authors.initials: BM
authors.lastname: Lee
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Cho
mesh_terms: D002244:Carbon / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006019:Glycolysis; D007218:Industrial Microbiology; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014974:Xanthomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D019298:Glucose-6-Phosphate; D002244:Carbon; D005947:Glucose; C002563:xanthan gum
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