title: NMDA receptor agonists fail to alter release from cerebellar basket cells.
abstract: Previous studies of NMDA receptor (NMDAR) expression on axons of cerebellar molecular layer interneurons have produced conflicting results. We made use of the calcium sensitivity of vesicular release machinery to test for NMDAR activity in basket cell axons. Iontophoresis of l-aspartate, an NMDAR agonist, onto basket cell axon collaterals had no effect on evoked IPSCs measured in synaptically coupled Purkinje cells. Furthermore, calcium indicators in basket cell varicosities did not report any change in intracellular calcium following iontophoresis of l-aspartate or two-photon uncaging of glutamate. In contrast, activation of presynaptic purinergic receptors by iontophoresis of ATP decreased evoked IPSC amplitudes and action potential-evoked calcium transients in axonal varicosities, demonstrating the effectiveness of activating presynaptic receptors by iontophoresis. We find no evidence for functional NMDARs in basket cell varicosities.
authors.affiliation: Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239, USA.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Pugh
authors.forename: Craig E
authors.initials: CE
authors.lastname: Jahr
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001224:Aspartic Acid / Q000494:pharmacology*; D001703:Biophysics; D002118:Calcium / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology*; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects; D007395:Interneurons / Q000187:drug effects*; D007478:Iontophoresis / Q000379:methods; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000187:drug effects / Q000502:physiology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists* / Q000502:physiology
chemical_list: C440256:4-methoxy-7-nitroindolinyl-glutamate; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D007211:Indoles; D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D001224:Aspartic Acid; D000255:Adenosine Triphosphate; D002118:Calcium
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