title: Adrenergic stimulation decreases osteoblast oxytocin synthesis.
abstract: Oxytocin (OT) regulates bone mass by inducing the differentiation of osteoblasts to a mature, mineralizing phenotype. We have shown recently that osteoblasts can synthesize OT. In view of known interactions between OT-ergic and adrenergic neurons in the central nervous system, we questioned whether the negative regulation of osteoblast differentiation by adrenergic nerves was mediated through its suppression of OT synthesis. We first confirmed that α(1b)  and β(2)  adrenergic receptors were expressed on both primary murine osteoblasts and MC3T3-E1 cells. We then showed that α(1)  and β(2)  adrenergic agonists downregulated OT synthesis, and that the effect of each agonist was reversed by its respective antagonist. The data suggest that the negative effects of adrenergic stimulation on bone mass could, in part, arise from decreased OT synthesis.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Cuscito
authors.forename: Graziana
authors.initials: G
authors.lastname: Colaianni
authors.forename: Roberto
authors.initials: R
authors.lastname: Tamma
authors.forename: Giovanni
authors.initials: G
authors.lastname: Greco
authors.forename: Stefania
authors.initials: S
authors.lastname: Dell'Endice
authors.forename: Tony
authors.initials: T
authors.lastname: Yuen
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Mone
authors.initials: M
authors.lastname: Zaidi
authors.forename: Adriana
authors.initials: A
authors.lastname: Di Benedetto
authors.forename: Alberta
authors.initials: A
authors.lastname: Zallone
mesh_terms: D059331:Adrenergic Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D058646:Adrenergic alpha-1 Receptor Agonists / Q000494:pharmacology*; D058666:Adrenergic beta-2 Receptor Agonists / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010121:Oxytocin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*
chemical_list: D058646:Adrenergic alpha-1 Receptor Agonists; D058666:Adrenergic beta-2 Receptor Agonists; D010121:Oxytocin