title: Recovery, survival, and function of transfused platelets and detection of platelet engraftment after allogeneic stem cell transplantation.
abstract: BACKGROUND
  Recovery and survival of transfused platelets (PLTs) are usually assessed by radioisotope labeling methods for evaluation of transfusion efficacy and new progress in the processing of PLT concentrates. Alternative, nonradioactive methods are warranted.
  
  
  METHODS
  A multicolor flow cytometry method was developed for simultaneous studies of recovery, survival, and function of transfused PLTs. Eight consecutive patients undergoing allogeneic stem cell transplantation (TX) were transfused with apheresis PLTs of nonself human leukocyte antigen (HLA) Class I types, and HLA Class I discrepancy between donor and recipient was used to identify transfused PLTs. Hematologic status and HLA Class I surface expression were analyzed immediately before transfusion, 1 and 6 hours after transfusion, and daily during the subsequent week. PLT activation was assessed by surface expression of CD63, CD62P, or CD42a, before and after stimulation with thrombin receptor agonist peptide.
  
  
  RESULTS
  PLT recovery was 43, 41, and 31% for fresh (5-72 hr old) and 30, 27, and 17% for stored (73-148 hr old) PLTs, after 1, 6, and 15 to 28 hours, respectively. Survival of fresh versus stored PLTs were 160 and 105 hours, respectively. Spontaneous PLT activation and residual activation potential were almost equal for fresh and stored PLTs. PLT engraftment was detected between Day 7 and Day 9, which was significantly earlier than first sign of neutrophil engraftment (Days 11-19; p=0.01).
  
  
  CONCLUSIONS
  Flow cytometry is an attractive alternative to radiolabeling of PLTs for simultaneous studies of survival, recovery, and function of transfused PLTs and early detection of PLT engraftment after allogeneic stem cell TX.
authors.affiliation: Department of Immunology and Transfusion Medicine, Oslo University Hospital HF, Ullevaal, Norway. annette.vetlesen@medisin.uio.no
authors.forename: Annette
authors.initials: A
authors.lastname: Vetlesen
authors.forename: Pål André
authors.initials: PA
authors.lastname: Holme
authors.forename: Torstein
authors.initials: T
authors.lastname: Lyberg
authors.forename: Jens
authors.initials: J
authors.lastname: Kjeldsen-Kragh
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D001800:Blood Specimen Collection / Q000379:methods; D002470:Cell Survival / Q000502:physiology; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods; D010983:Plateletpheresis / Q000379:methods; D020127:Recovery of Function / Q000502:physiology; D013997:Time Factors; D014184:Transplantation, Homologous; D055815:Young Adult