title: Matrix metalloproteinase-2 expression induced by two different adhesive systems on human pulp fibroblasts.
abstract: BACKGROUND
  This study evaluated the expression of matrix metalloproteinase-2 (MMP-2) in primary cultures of human pulp fibroblasts (HPFs) when exposed to extracts from dentin-bonding systems.
  
  
  METHODS
  Polymerized resin disks of the bonding agent of a 2-step self-etch adhesive (TechBond, Isasan, Rovello Porro, Italy) or of the primer/bonding agent a 2-step etch-and-rinse adhesive (Optibond Solo; Sybron-Kerr, Orange, CA) were immersed in HPF culture medium for 24 or 96 hours. HPFs were incubated in the adhesive-conditioned or control (untreated) culture medium for 24 hours. Western blot and immunofluorescence analyses were performed to assay MMP-2 expression.
  
  
  RESULTS
  MMP-2 expression levels in HPFs cultured for 24 hours in culture medium were similar in both the control and experimental media groups showing a faint band at 67 kDa. Conversely, the HPFs incubated in the medium that contain polymerized resin disks for 96 hours showed increased MMP-2 expression compared with the untreated medium. The self-etch adhesive displayed the most pronounced induction of MMP-2 expression. These findings were confirmed by immunofluorescence analysis.
  
  
  CONCLUSIONS
  HPFs display increased MMP-2 expression after 96 hours of conditioning of the HPF culture medium with polymerized disks of dentin bonding systems. This MMP-2 expression/activation may represent a defence mechanism exhibited by HPFs towards monomers eluted from the dentin bonding systems.
authors.affiliation: Department of Clinical Sciences and Stomatology, Marche Polytechnic University, Ancona, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Orsini
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.forename: Monia
authors.initials: M
authors.lastname: Orciani
authors.forename: Angelo
authors.initials: A
authors.lastname: Putignano
authors.forename: Maurizio
authors.initials: M
authors.lastname: Procaccini
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D015153:Blotting, Western; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D018093:Gelatinases / Q000032:analysis; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D019279:Resin Cements / Q000494:pharmacology; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D017220:Dentin-Bonding Agents; D004792:Enzyme Precursors; C428805:OptiBond SOLO; D019279:Resin Cements; D017438:Bisphenol A-Glycidyl Methacrylate; D018093:Gelatinases; C069860:progelatinase; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2