title: Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference.
abstract: A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viability when the cells are subjected to replication interference. Here we show that Drosophila S2 cells do not appear to show the same degree of sensitivity to MCM2-6 reduction. Under normal cell growth conditions a reduction of >95% in the levels of MCM3, 5, and 6 causes no significant short term alteration in the parameters of DNA replication or increase in DNA damage. MCM depleted cells challenged with HU do show a decrease in the density of replication forks compared to cells with normal levels of MCM proteins, but this produces no consistent change in the levels of DNA damage observed. In contrast a comparable reduction of MCM7 levels has marked effects on viability, replication parameters and DNA damage in the absence of HU treatment.
authors.affiliation: Department Basic Medical Sciences, St. Georges University London, London, United Kingdom.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Crevel
authors.forename: Gilles
authors.initials: G
authors.lastname: Crevel
authors.forename: Thierry
authors.initials: T
authors.lastname: Gostan
authors.forename: Christelle
authors.initials: C
authors.lastname: de Renty
authors.forename: Vincent
authors.initials: V
authors.lastname: Coulon
authors.forename: Sue
authors.initials: S
authors.lastname: Cotterill
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D004261:DNA Replication; D004330:Drosophila / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D064151:Minichromosome Maintenance Complex Component 6; D064110:Minichromosome Maintenance Proteins; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C489426:Mcm5 protein, Drosophila; D019384:Nucleic Acid Synthesis Inhibitors; C555716:MCM2 protein, Drosophila; C491204:Mcm6 protein, Drosophila; D064151:Minichromosome Maintenance Complex Component 6; D064110:Minichromosome Maintenance Proteins; D006918:Hydroxyurea
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