title: [Effects of iptakalim on pressure-overload induced cardiac remodeling and plasma PGI2 content in rats].
abstract: OBJECTIVE
  To study the effects of iptakalim (IPT) on pressure-overload induced cardiac remodeling in rats, and investigate correlation between this protection effects and plasma PGI2 content.
  
  
  METHODS
  The pressure-overload induced cardiac remodeling model was induced by abdominal aorta constriction for 6 weeks, and the rats were divided into 5 groups repectively: (1) sham group, (2) control group, (3) IPT 3 mg/kg group (IPT 3), (4) indomethacin 2 mg/kg group (Indo 2), (5) indomethacin 2 mg/kg + IPT 3 mg/kg group (Indo 2 + IPT 3). RM6000 eight channel physiological recorder was used to record haemodynamics index, heart weight was weighed and the cardiac remodeling index was calculated, HE stain and Masson's stain were employed to perform histological analysis, colorimetric method was used to detect the hydroxyproline content in cardiac tissue, radioimmunological method was used to measure the plasma PGI2 content.
  
  
  RESULTS
  After 42 days of aortic banding, the hyperdynamic circulation state, cardiac remodeling and decreased plasma PGI2 content were observed in the model group compared with those in the sham group, which were effectively reserved by treatment with IPT 3 mg/kg. Single-use indomethacin led to further deterioration of this pathophysiological changes, however, combination administration of IPT 3 mg/kg prevented these from worsening characteristic by ameliorating hyperdynamic circulation state and cardiac remodeling, augmnent plasma PGI2 content.
  
  
  CONCLUSIONS
  IPT can significantly reverse abdominal aorta binding/pressure-overload induced cardiac remodeling, its mechanism may contribute to binding K(ATP) channel in endothelial cells, ameliorating endothelium cells function, augmenting PGI2 synthesis and secretion.
authors.affiliation: Department of Phannrmacology, Basic College, Key Laboratory of Anti-inflammatory and Immunophannrmacology of Education Ministry, Anhui Medical University, Hefei 230032.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Chao-liang
authors.initials: CL
authors.lastname: Long
authors.forename: Ru-huan
authors.initials: RH
authors.lastname: Wang
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D003250:Constriction; D004730:Endothelium, Vascular / Q000378:metabolism / Q000502:physiology; D011464:Epoprostenol / Q000097:blood*; D006973:Hypertension / Q000097:blood / Q000503:physiopathology*; D054086:KATP Channels / Q000187:drug effects; D008297:Male; D011437:Propylamines / Q000494:pharmacology*; D051381:Rats; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D054086:KATP Channels; C502260:N-(1-methylethyl)-1,1,2-trimethylpropylamine; D011437:Propylamines; D011464:Epoprostenol