title: Enhanced histopathology of the immune system: a review and update.
abstract: Enhanced histopathology (EH) of the immune system is a tool that the pathologist can use to assist in the detection of lymphoid organ lesions when evaluating a suspected immunomodulatory test article within a subchronic study or as a component of a more comprehensive, tiered approach to immunotoxicity testing. There are three primary points to consider when performing EH: (1) each lymphoid organ has separate compartments that support specific immune functions; (2) these compartments should be evaluated individually; and (3) semiquantitative descriptive rather than interpretive terminology should be used to characterize any changes. Enhanced histopathology is a screening tool that should be used in conjunction with study data including clinical signs, gross changes, body weight, spleen and thymus weights, other organ or tissue changes, and clinical pathology. Points to consider include appropriate tissue collection, sectioning, and staining; lesion grading; and diligent comparison with concurrent controls. The value of EH of lymphoid organs is to aid in the identification of target cell type, changes in cell production and cell death, changes in cellular trafficking and recirculation, and determination of mechanism of action.
authors.affiliation: National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. elmore@niehs.nih.gov
authors.forename: Susan A
authors.initials: SA
authors.lastname: Elmore
mesh_terms: D000818:Animals; D017408:Guidelines as Topic; D006652:Histological Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D007107:Immune System / Q000473:pathology*; D010338:Pathology, Clinical / Q000379:methods* / Q000592:standards; D014116:Toxicology / Q000379:methods* / Q000592:standards
references.citation: Toxicol Pathol. 2006;34(5):599-608
references.pmid: 17067945
references.citation: Toxicology. 1994 Feb 7;86(3):187-212
references.pmid: 8128503
references.citation: Fundam Appl Toxicol. 1993 Jul;21(1):71-82
references.pmid: 8365588
references.citation: Toxicol Sci. 2004 Mar;78(1):107-15
references.pmid: 14691208
references.citation: Toxicol Pathol. 2006;34(5):548-65
references.pmid: 17067943
references.citation: Toxicol Pathol. 2006;34(5):656-65
references.pmid: 17067951
references.citation: Exp Toxicol Pathol. 2005 Aug;57(1):1-5
references.pmid: 16089314
references.citation: Fundam Appl Toxicol. 1988 Jan;10(1):2-19
references.pmid: 3280374
references.citation: Toxicology. 1998 Feb 6;125(2-3):183-201
references.pmid: 9570332
references.citation: Toxicol Pathol. 2006;34(5):455-65
references.pmid: 17067939
references.citation: Toxicol Sci. 2004 Dec;82(2):504-14
references.pmid: 15342959
references.citation: Toxicology. 2003 Jun 3;188(1):49-71
references.pmid: 12748041
references.citation: Toxicol Pathol. 2006;34(5):504-14
references.pmid: 17067941
references.citation: Toxicol Pathol. 2006;34(5):634-47
references.pmid: 17067949
references.citation: Arch Toxicol Suppl. 1980;4:95-108
references.pmid: 7002113
references.citation: Toxicol Pathol. 2006;34(5):666-86
references.pmid: 17067952
references.citation: Methods Mol Biol. 2010;598:323-39
references.pmid: 19967523
references.citation: Toxicol Pathol. 2006;34(5):687-96
references.pmid: 17067953
references.citation: J Anat. 1971 Sep;109(Pt 3):369-83
references.pmid: 5153800
references.citation: Toxicol Pathol. 2006;34(5):409-24
references.pmid: 17067937
references.citation: Toxicol Pathol. 2000 May-Jun;28(3):454-66
references.pmid: 10862566
references.citation: Toxicol Pathol. 2006;34(5):648-55
references.pmid: 17067950
references.citation: Food Chem Toxicol. 1995 Mar;33(3):239-43
references.pmid: 7896235
references.citation: Toxicol Pathol. 2005;33(3):404-7; discussion 408
references.pmid: 15805080
references.citation: Fundam Appl Toxicol. 1992 Feb;18(2):200-10
references.pmid: 1534777