title: Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli.
abstract: To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.
authors.affiliation: Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Karl
authors.initials: K
authors.lastname: Skoog
authors.forename: Bill
authors.initials: B
authors.lastname: Söderström
authors.forename: Jerker
authors.initials: J
authors.lastname: Widengren
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Daley
mesh_terms: D002455:Cell Division; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019897:Periplasm / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
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