title: Dose-response effects of an aqueous formulation of calcitriol and evaluation of renal function in nonpregnant cows.
abstract: Dose responses of plasma calcitriol, calcium (Ca), bone metabolic markers and glomerular filtration rate (GFR) were evaluated in four nonpregnant Holstein cows treated subcutaneously with an aqueous formulation of calcitriol at four doses in a 4 × 4 Latin-square design. Calcitriol, Ca, and markers of bone metabolism were analyzed in plasma samples. GFR was measured in predose and day 5 samples. Plasma calcitriol and Ca concentrations increased dose-dependently. The calcitriol dose was positively correlated with the area under the concentration-time curve of plasma calcitriol. Bone formation markers tended to increase from day 3 onward for all doses. No significant changes in GFR were noted. Thus, exogenous calcitriol administered between 0.0625 and 0.5 µg/kg body weight elicited dose-dependent increases in both plasma calcitriol and Ca and elevated bone formation markers without affecting renal function in nonpregnant cows.
authors.affiliation: United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Danil
authors.initials: D
authors.lastname: Kim
authors.forename: Norio
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Izumi
authors.initials: I
authors.lastname: Abe
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
authors.forename: Bhuminand
authors.initials: B
authors.lastname: Devkota
authors.forename: Kouya
authors.initials: K
authors.lastname: Sasaki
authors.forename: Norimoto
authors.initials: N
authors.lastname: Okura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ohashi
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D001806:Blood Urea Nitrogen; D002117:Calcitriol / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D003404:Creatinine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006909:Hydroxyproline / Q000097:blood; D007527:Isoenzymes / Q000097:blood; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015675:Osteocalcin / Q000097:blood; D018709:Statistics, Nonparametric; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D015675:Osteocalcin; D003404:Creatinine; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002117:Calcitriol; D006909:Hydroxyproline; D002118:Calcium