title: The complete sequence of tobacco mosaic virus isolate Ohio V reveals a high accumulation of silent mutations in all open reading frames.
abstract: TMVOhioV was first described 1969 by [1] because it did break resistance of tomato breeding lines containing Tm-1- and Tm-2 resistance genes. It was obtained 1987 from Wetter (Saarbrücken, Germany) and transferred into the DSMZ-Plant Virus Collection (Braunschweig, Germany). A partial sequence of TMVOhioV, the CP gene, has been reported [11] and its comparison with a TMV type isolates (TMVtype), e.g. EMBL: V01409, revealed 50 point mutations in a total of 477 nucleotides (nts) leading to the replacement of only 7 amino acids (aa). In order to investigate the mutations in the non-translated regions and the number of silent mutation in the three other open reading frames (ORF), we sequenced the complete genome of isolate TMVOhioV and compared it to those of other Tobamoviruses.
authors.affiliation: Biocenter Klein Flottbek, University of Hamburg, Ohnhorststr. 18, Hamburg 22609, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Körbelin
authors.forename: Peter
authors.initials: P
authors.lastname: Willingmann
authors.forename: Günter
authors.initials: G
authors.lastname: Adam
authors.forename: Cornelia
authors.initials: C
authors.lastname: Heinze
mesh_terms: D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D017354:Point Mutation; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins