title: Hypothalamic gene expression in ω-3 PUFA-deficient male rats before, and following, development of hypertension.
abstract: Dietary deficiency of ω-3 fatty acids (ω-3 DEF) produces hypertension in later life. This study examined the effect of ω-3 DEF on blood pressure and hypothalamic gene expression in young rats, before the development of hypertension, and in older rats following the onset of hypertension. Animals were fed experimental diets that were deficient in ω-3 fatty acids, sufficient in short-chain ω-3 fatty acids or sufficient in short- and long-chain ω-3 fatty acids, from the prenatal period until 10 or 36 weeks-of-age. There was no difference in blood pressure between groups at 10 weeks-of-age; however, at 36 weeks-of-age ω-3 DEF animals were hypertensive in relation to sufficient groups. At 10 weeks, expression of angiotensin-II(1A) receptors and dopamine D(3) receptors were significantly increased in the hypothalamic tissue of ω-3 DEF animals. In contrast, at 36 weeks, α(2a) and β(1) adrenergic receptor expression was significantly reduced in the ω-3 DEF group. Brain docosahexaenoic acid was significantly lower in ω-3 DEF group compared with sufficient groups. This study demonstrates that dietary ω-3 DEF causes changes both in the expression of key genes involved in central blood pressure regulation and in blood pressure. The data may indicate that hypertension resulting from ω-3 DEF is mediated by the central adrenergic system.
authors.affiliation: School of Medicine, Deakin University, Geelong, Victoria, Australia.
authors.forename: Denovan P
authors.initials: DP
authors.lastname: Begg
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
authors.forename: Klara
authors.initials: K
authors.lastname: Kitajka
authors.forename: Dalma
authors.initials: D
authors.lastname: Ménesi
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Allen
authors.forename: Duo
authors.initials: D
authors.lastname: Li
authors.forename: Michael L
authors.initials: ML
authors.lastname: Mathai
authors.forename: Jian R
authors.initials: JR
authors.lastname: Shi
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Sinclair
authors.forename: Richard S
authors.initials: RS
authors.lastname: Weisinger
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000235:genetics*; D018450:Disease Progression; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D015870:Gene Expression; D006973:Hypertension / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics / Q000378:metabolism; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics / Q000378:metabolism; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics / Q000378:metabolism; D011954:Receptors, Dopamine / Q000235:genetics / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D044140:Receptor, Angiotensin, Type 1; D018341:Receptors, Adrenergic, alpha-2; D018342:Receptors, Adrenergic, beta-1; D011954:Receptors, Dopamine