title: Structure characterisation of a α β-glucan polysaccharide from Auricularia polytricha.
abstract: A water-soluble α β-glucan with a molecular weight of 1.62 × 10(5) Da, AAPS -1, was obtained from the fruiting bodies of the edible mushroom Auricularia polytricha by a combined separation of high-speed countercurrent chromatography (HSCCC)-Sephacryl S-300 HR column chromatography. The structure of AAPS-1 was elucidated that the polysaccharide possesses a backbone composed of (1 → 6)-linked-α-D-glucopyranosyl and (1 → 6)-linked-β-D-glucopyranosyl residues, partially substituted at O-3 of β-D-glucopyranosyl residue by side chain (1 → 4)-α-D-Glcp and terminated with non-reducing end α-D-Glcp-(1 → on the basis of the analyses of partial hydrolysis, periodate oxidation, acetylation, methylation and NMR spectroscopy ((1)H, (13)C).
authors.affiliation: Institute of Food and Biological Engineering, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang, China.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Song
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Du
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D002138:Calibration; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010945:Plants, Edible / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D047071:beta-Glucans