title: Purification and characterization of a novel angiotensin-I converting enzyme (ACE) inhibitory peptide derived from enzymatic hydrolysate of grass carp protein.
abstract: Peptides inhibiting angiotensin-I converting enzyme (ACE, EC. 3.4.15.1) are possible cures of hypertension. Food-derived ACE-inhibitory peptides are particularly attractive because of reduced side effects. Previously, we reported ACE-inhibitory activity of grass carp protein hydrolysates. In this work, we report steps for purifying the ACE-inhibitory peptide from the hydrolysate and its biochemical properties. Following steps of ultrafiltration, macroporous adsorption resin, and two steps of reversed phase high performance liquid chromatography (RE-HPLC), a single Val-Ala-Pro (VAP) tripeptide was identified. The tripeptide with excellent ACE-inhibitory activity (IC(50) value of 0.00534 mg/mL) was a competitive ACE inhibitor and stable against both ACE and gastrointestinal enzymes of pepsin and chymotrypsin. This is the first report of food-derived VAP. The identified unique biochemical properties of VAP may enable the application of grass carp protein hydrolysates as a functional food for treatments of hypertension. The developed purification conditions also allow the production of VAP for pharmaceutical applications.
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China. jiwangchen@yahoo.com.cn
authors.forename: Jiwang
authors.initials: J
authors.lastname: Chen
authors.forename: Yimei
authors.initials: Y
authors.lastname: Wang
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zhong
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
authors.forename: Wenshui
authors.initials: W
authors.lastname: Xia
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D002347:Carps; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002918:Chymotrypsin / Q000378:metabolism; D005374:Filtration; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D010434:Pepsin A / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D002918:Chymotrypsin; D010434:Pepsin A