title: PAF and its metabolic enzymes in healthy volunteers: interrelations and correlations with basic characteristics.
abstract: PAF (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent inflammatory mediator, is synthesized via the remodeling and the de novo route, key enzymes of which are acetyl-CoA:lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1-alkyl-2-acetyl-sn-glycerol cholinephosphotransferase (PAF-CPT), respectively. PAF-acetylhydrolase (PAF-AH) and its extracellular isoform lipoprotein-associated phospholipase-A(2) (Lp-PLA(2)) catabolize PAF. This study evaluated PAF levels together with leukocyte PAF-CPT, lyso-PAF-AT, PAF-AH and Lp-PLA(2) activities in 106 healthy volunteers. Men had lower PAF levels and higher activity of both catabolic enzymes and lyso-PAF-AT than women (P-values <0.05). Age was inversely correlated with PAF levels in men (r=-0.279, P=0.06) and lyso-PAF-AT in women (r=-0.280, P=0.05). In contrast, Lp-PLA(2) was positively correlated with age (r=0.201, P=0.04). Moreover, PAF-CPT was positively correlated with glucose (r=0.430, P=0.002) in women. In addition, Principal Component Analysis revealed three PAF metabolic patterns: (i) increased activities of PAF-CPT and PAF-AH, (ii) increased activities of PAF-CPT and lyso-PAF-AT and (iii) increased activity of Lp-PLA(2). The present study underlines the complexity of PAF's metabolism determinants.
authors.affiliation: Department of Nutrition-Dietetics, Harokopio University, 70 El. Venizelou Street, 17671, Athens, Greece.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Detopoulou
authors.forename: Tzortzis
authors.initials: T
authors.lastname: Nomikos
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Fragopoulou
authors.forename: George
authors.initials: G
authors.lastname: Stamatakis
authors.forename: Demosthenes B
authors.initials: DB
authors.lastname: Panagiotakos
authors.forename: Smaragdi
authors.initials: S
authors.lastname: Antonopoulou
mesh_terms: D000328:Adult; D000375:Aging / Q000097:blood / Q000378:metabolism / Q000502:physiology; D055162:Biocatalysis; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D006262:Health; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D050356:Lipid Metabolism; D008297:Male; D010972:Platelet Activating Factor / Q000378:metabolism*; D012727:Sex Characteristics
chemical_list: D001786:Blood Glucose; D004798:Enzymes; D010972:Platelet Activating Factor