title: [Analysis of the transcriptional profiling of cell cycle regulatory networks of recombinant Chinese hamster ovary cells in batch and fed-batch cultures].
abstract: In the light of Chinese hamster ovary (CHO) cell line 11G-S expressing human recombinant pro-urokinase, the differences of gene expression levels of the cells in different growth phases in both batch and fed-batch cultures were revealed by using gene chip technology. Then, based on the known cell cycle regulatory networks, the transcriptional profiling of the cell cycle regulatory networks of the cells in batch and fed-batch cultures was analyzed by using Genmapp software. Among the approximate 19 191 target genes in gene chip, the number of down-regulated genes was more than those of up-regulated genes of the cells in both batch and fed-batch cultures. The number of down-regulated genes of the cells in the recession phase in fed-batch culture was much more than that of the cells in batch culture. Comparative transcriptional analysis of the key cell cycle regulatory genes of the cells in both culture modes indicated that the cell proliferation and cell viability of the cells in both batch and fed-batch cultures were mainly regulated through down-regulating Cdk6, Cdk2, Cdc2a, Ccne1, Ccne2 genes of CDKs, Cyclin and CKI family and up-regulating Smad4 gene.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xingmao
authors.initials: X
authors.lastname: Liu
authors.forename: Lingling
authors.initials: L
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Shichong
authors.initials: S
authors.lastname: Li
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Benchuan
authors.initials: B
authors.lastname: Wu
authors.forename: Zhaolie
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D016466:CHO Cells; D018797:Cell Cycle Proteins / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D051901:Smad4 Protein / Q000235:genetics; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D011994:Recombinant Proteins; D051901:Smad4 Protein; D051357:Cyclin-Dependent Kinase 2; D051361:Cyclin-Dependent Kinase 6; D014568:Urokinase-Type Plasminogen Activator; C067551:saruplase