title: [Effects of different concentrations of gubishu containing serum on the proliferation of rabbit articular chondrocytes in vitro culture].
abstract: OBJECTIVE
  To investigate the effects of different concentrations of Gubishu containing serum on the proliferation of rabbit articular chondrocytes cultured in vitro.
  
  
  METHODS
  Articular chondrocytes were obtained from the cartilage of 1-month rabbit and cultured in vitro. They were randomly divided into 8 groups,blank and Gubishu groups in different concentrations (5%, 10%,15%, 20%), MTT assay method was adopted to observe the influence of Gubishu containing serum with different concentrations to the proliferation of chondrocytes after incubated 1, 3, 5, 7 and 9 days.
  
  
  RESULTS
  The proliferation of chondrocytes was dependent on the concentration in Gubishu groups. At same time point,there was significant value between every groups, 20% concentration was greatest (P<0.05); There was significant differences between 5%, 10% and 20% concentration of the blank groups at same time point (P<0.05), and was not between 15% and 20% concentration at the 1, 3, 5 and 7 days (P>0.05), 20% concentration of the blank group was greatest. 20% concentrations of Gubishu containing serum was significantly greater than 20% concentrations of blank group at the 1, 3, 5 and 7 days (P<0.05).
  
  
  CONCLUSIONS
  20% concentrations of Gubishu containing serum can significantly increase the proliferation of chondrocytes, and bring the logarithmic growth period forward to the 3 day.
authors.affiliation: Fujian University of Traditional Chinese Medicine, Fujian, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Qin
authors.forename: He-ming
authors.initials: HM
authors.lastname: Wang
authors.forename: Yu-qian
authors.initials: YQ
authors.lastname: Lou
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D011817:Rabbits; D044967:Serum
chemical_list: D004365:Drugs, Chinese Herbal