title: Invariant natural killer T cells in chronic obstructive pulmonary disease.
abstract: OBJECTIVE
  Invariant natural killer T (iNKT) cells may play an important role in regulating the innate and acquired immune systems in chronic obstructive pulmonary disease (COPD). However, there is little information regarding the potential role of iNKT cells in the pathogenesis of COPD. To investigate whether iNKT cells have an important role in COPD, the frequency of iNKT cells in peripheral blood of patients with COPD was analysed.
  
  
  METHODS
  This was a comparative study of 28 patients with COPD and 19 age-matched healthy control subjects. Blood iNKT cells were stained with 6B11 mAb, anti-T cell receptor Vα24 mAb, anti-T cell receptor Vβ11 mAb or α-galactosylceramide-loaded CD1d-tetramer, and analysed by flow cytometry.
  
  
  RESULTS
  The frequency of CD4(+) 6B11(+) iNKT, CD4(+) Vα24(+) iNKT, CD4(+) Vβ11(+) iNKT and CD3(+) 6B11(+) iNKT cells was significantly lower in peripheral blood of patients with COPD than in that of healthy control subjects. The frequency of CD4(+) 6B11(+) iNKT cells was significantly lower in patients with exacerbations of COPD compared with those with stable COPD.
  
  
  CONCLUSIONS
  The frequency of iNKT was decreased in peripheral blood of patients with COPD. These results strongly suggest that iNKT cells may play an important role in the pathogenesis of COPD.
authors.affiliation: Department of Internal Medicine, Chonnam National University Hospital, Kwangju, South Korea.
authors.forename: Su Young
authors.initials: SY
authors.lastname: Chi
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Ban
authors.forename: Yong Soo
authors.initials: YS
authors.lastname: Kwon
authors.forename: In Jae
authors.initials: IJ
authors.lastname: Oh
authors.forename: Kyu Sik
authors.initials: KS
authors.lastname: Kim
authors.forename: Yu Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Lim
mesh_terms: D000368:Aged; D018949:Antigens, CD1 / Q000276:immunology; D018791:CD4 Lymphocyte Count; D005260:Female; D005434:Flow Cytometry; D005699:Galactosylceramides / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000276:immunology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology*; D012737:Sex Factors
chemical_list: D018949:Antigens, CD1; D005699:Galactosylceramides; C493154:alpha-galactosylceramide