title: A new furostanol saponin from Asparagus cochinchinensis.
abstract: A new furostanol saponin, (25S)-26-O-β-D-glucopyranosyl-5β-furost-20(22)-en-3β, 15β,26-triol-3-O-[α-L-rhamnopyranosyl-(1-4)]-β-D: -glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5β-spirostan-3β-yl-O-[O-α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, (1)H-NMR, (13)C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC(50) value of 3.87 μg/mL.
authors.affiliation: Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Cong-Li
authors.initials: CL
authors.lastname: Xu
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Xuan
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Li
authors.forename: Run-Hui
authors.initials: RH
authors.lastname: Liu
authors.forename: Xi-Ke
authors.initials: XK
authors.lastname: Xu
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D027761:Asparagus Plant / Q000737:chemistry*; D045744:Cell Line, Tumor; D002849:Chromatography, Gas; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D010936:Plant Extracts; D012503:Saponins; D013256:Steroids; D013778:Tetrazolium Salts; D013844:Thiazoles; C411448:timosaponin E1; C022616:thiazolyl blue