title: Preparation of encapsulated alliinase in alginate microparticles.
abstract: Alliinase (alkylsulphenate lyase, EC 4.4.1.4), which catalyses the production of allicin, was immobilized in alginate microparticles. Addition of pyridoxal 5'-phosphate to the microparticles enhanced alliinase activity. Encapsulated alliinase were significantly higher (30 and 22%, respectively) than those of non-encapsulated alliinase at 60°C and at pH 2. Therefore, microencapsulation of alliinase with alginate can offer an effective way of sustaining enzyme activity during oral administration and passage through the stomach.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: J A
authors.initials: JA
authors.lastname: Ko
authors.forename: Y L
authors.initials: YL
authors.lastname: Lee
authors.forename: H J
authors.initials: HJ
authors.lastname: Jeong
authors.forename: H J
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011730:Pyridoxal / Q000031:analogs & derivatives / Q000378:metabolism; D013441:Sulfinic Acids / Q000378:metabolism; D013696:Temperature
chemical_list: D000464:Alginates; D002214:Capsules; D004220:Disulfides; D020536:Enzyme Activators; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D013441:Sulfinic Acids; C006452:allicin; D011730:Pyridoxal; C036319:pyridoxal 5'-sulfate; D020723:Glucuronic Acid; D013437:Carbon-Sulfur Lyases; C019243:alliin lyase