title: Mobilization of lymphoblasts from bone marrow to peripheral blood in childhood acute lymphoblastic leukaemia: role of 9-O-acetylated sialoglycoproteins.
abstract: Childhood acute lymphoblastic leukaemia is characterized by aberrant proliferation and accumulation of malignant lymphoblasts in bone marrow (BM), followed by their migration into circulation. An enhanced cell-surface expression of ALL-associated 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) was demonstrated. Present investigation reports a positive correlation between the increased density of Neu5,9Ac(2)-GPs on lymphoblasts and their mobilization from BM involving enhanced Neu5,9Ac(2) on CD45 demonstrating modulation of FAK and ERK molecules. In contrast, a small population of cells, identified as haematopoietic precursors, with comparatively lesser Neu5,9Ac(2)-GPs showed increased binding towards BM stroma. Thus, Neu5,9Ac(2)-GPs is a developmentally regulated oncofoetal antigen, whose up-regulation is imperative in the interaction between lymphoblasts and BM stroma, governing their mobilization into circulation.
authors.affiliation: Cancer and Cell Biology Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology, Kolkata, West Bengal, India.
authors.forename: Suchandra
authors.initials: S
authors.lastname: Chowdhury
authors.forename: Chandan
authors.initials: C
authors.lastname: Mandal
authors.forename: Sayantani
authors.initials: S
authors.lastname: Sarkar
authors.forename: Arup Kumar
authors.initials: AK
authors.lastname: Bag
authors.forename: Reinhard
authors.initials: R
authors.lastname: Vlasak
authors.forename: Sarmila
authors.initials: S
authors.lastname: Chandra
authors.forename: Chitra
authors.initials: C
authors.lastname: Mandal
mesh_terms: D000107:Acetylation; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000473:pathology*; D011379:Prognosis; D012794:Sialic Acids / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation
chemical_list: D012794:Sialic Acids; D012795:Sialoglycoproteins; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human