title: Liquid chromatography tandem mass spectrometry determination of total budesonide levels in dog plasma after inhalation exposure.
abstract: A sensitive and selective method to quantify budesonide in dog plasma samples was developed and fully validated. Liquid-liquid extraction was followed by solid-phase extraction and liquid chromatography-tandem mass spectrometry with electrospray ionization. After reconstitution of the analytes in the mobile phase, samples were analysed by reversed-phase liquid chromatography with isocratic elution. d8-Budesonide was used as an internal standard, and characteristic transitions of d8-budesonide and budesonide were used for quantification. The method was validated with respect to selectivity, specificity, linearity, recovery, repeatability, reproducibility and limits of detection and quantification. The validated method was successfully applied to monitor the plasma levels of budesonide in dogs exposed to clinical doses of inhaled and intravenous drug.
authors.affiliation: Finnish Food Safety Authority Evira, Chemistry and Toxicology Research Unit, Mustialankatu 3, 00790 Helsinki, Finland. seija.berg@evira.fi
authors.forename: Seija
authors.initials: S
authors.lastname: Berg
authors.forename: Marika
authors.initials: M
authors.lastname: Melamies
authors.forename: Minna
authors.initials: M
authors.lastname: Rajamäki
authors.forename: Outi
authors.initials: O
authors.lastname: Vainio
authors.forename: Kimmo
authors.initials: K
authors.lastname: Peltonen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000097:blood*; D019819:Budesonide / Q000008:administration & dosage / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods; D004285:Dogs; D019570:Inhalation Exposure; D059625:Liquid-Liquid Extraction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000893:Anti-Inflammatory Agents; D019819:Budesonide