title: AGPAT6 polymorphism and  its association with milk traits of dairy goats.
abstract: As one  of the eight members in the 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs)  family, AGPAT6 is a crucial enzyme for the biosynthesis of glycerolipids  and triacylglycerol in eukaryotes, as well as catalyzing the conversion from  lysophosphatidic acid to phosphatidic acid. AGPAT6 can be considered as  a candidate gene for regulating milk composition. DNA sequencing and PCR-RFLP  methods were applied to detect genetic variation in the AGPAT6 gene in  549 Chinese dairy goats. Four polymorphisms (NC_007328.3:g.152G>C,  8124G>A, 9263C>G, 16436G>A) were detected in 5'UTR, intron 2, exon 4,  and 3'UTR, respectively. For the KpnΙ locus, the frequencies of the AGPAT6-G allele  were 0.955 and 0.936 for SN (Xinong Sannen) and GZ (Guanzhong) dairy goat  breeds, respectively. In the PCR-RFLP analysis for KpnΙ, EcoRII, NcoΙ, and BglΙ, the frequencies of the G allele  of AGPAT6 were 0.955 and 0.936, 0.694 and 0.819, 0.206 and 0.254, 0.729  and 0.623 for SN and GZ dairy goat breeds, respectively. The 9263C>G  mutation revealed a synonymous genetic code of Thr (threonine). Associations  between the four mutations and milk traits were analyzed in two dairy goat  breeds. At the 9263C>G locus, genotype GG and CG individuals showed  significantly better milk performance than genotype CC individuals (P <  0.05). Therefore, the G allele is suggested to be a molecular marker for milk  production in dairy goats.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
authors.forename: C
authors.initials: C
authors.lastname: He
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Chang
authors.forename: B L
authors.initials: BL
authors.lastname: Guo
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: X P
authors.initials: XP
authors.lastname: Yue
authors.forename: X Y
authors.initials: XY
authors.lastname: Lan
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000483:Alleles; D019943:Amino Acid Substitution; D000818:Animals; D001947:Breeding; D005091:Exons / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers; D005838:Genotype; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D007438:Introns / Q000235:genetics; D008892:Milk; D020125:Mutation, Missense; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D005819:Genetic Markers; D005992:Glycerol-3-Phosphate O-Acyltransferase