title: CCL21 modulates the migration of NSCL cancer by changing the concentration             of intracellular Ca2+.
abstract: Recurrence and metastasis are the major factors associated with the poor             prognosis of non-small cell lung cancer (NSCLC). It has been shown that multiple             chemokines and their receptors are related to the progression and metastasis of             NSCLC. The aim of this study was to conduct an investigation into whether CCL21             and its receptor, CCR7, play a role in NSCLC invasion and metastasis. We used             Western blotting, immunocytochemistry and flow cytometry to detect CCR7 protein             expression in four NSCLC cell lines EKVX, HOP-62, NCI-H23 and Slu-01; and we conducted             a cell migration experiment to observe the pseudopodia formation and mobility             of the lung cancer cells. The concentration of intracellular calcium was measured             by fluorescence microscopy. CCR7 protein was positively expressed in the four             NSCLC cell lines EKVX, HOP-62, NCI-H23 and Slu-01. Following CCL21 stimulation,             obvious pseudopodia formation of lung cancer cells was observed. The cell migration             experiment showed that following incubation with CCL21, the number of EKVX cells             which passed through the polycarbonate micro-porous filter membranes also increased             to an obvious extent. After CCL21 incubation, the intracellular Ca2+ level of             the EKVX cells increased to an obvious extent. Chemokine CCL21 facilitates the             migration of lung cancer by changing the concentration of intracellular Ca2+.             The CCL21-CCR7 axis may play an important role in NSCLC invasion and metastasis.             It may also be a potential target for NSCLC therapy or for prevention of the recurrence             and metastasis of NSCLC.
authors.affiliation: Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Changli
authors.initials: C
authors.lastname: Wang
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D054421:Chemokine CCL21 / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D054400:Receptors, CCR7 / Q000378:metabolism
chemical_list: D054421:Chemokine CCL21; D054400:Receptors, CCR7; D002118:Calcium