title: A novel functionally distinct subtype of striatal neuropeptide Y interneuron.
abstract: We investigated the properties of neostriatal neuropeptide Y (NPY)-expressing interneurons in transgenic GFP (green fluorescent protein)-NPY reporter mice. In vitro whole-cell recordings and biocytin staining demonstrated the existence of a novel class of neostriatal NPY-expressing GABAergic interneurons that exhibit electrophysiological, neurochemical, and morphological properties strikingly different from those of previously described NPY-containing, plateau-depolarization low-threshold spike (NPY-PLTS) interneurons. The novel NPY interneuron type (NPY-neurogliaform) differed from previously described NPY-PLTS interneurons by exhibiting a significantly lower input resistance and hyperpolarized membrane potential, regular, nonaccommodating spiking in response to depolarizing current injections, and an absence of plateau depolarizations or low-threshold spikes. NPY-neurogliaform interneurons were also easily distinguished morphologically by their dense, compact, and highly branched dendritic and local axonal arborizations that contrasted sharply with the sparse and extended axonal and dendritic arborizations of NPY-PLTS interneurons. Furthermore, NPY-neurogliaform interneurons did not express immunofluorescence for somatostatin or nitric oxide synthase that was ubiquitous in NPY-PLTS interneurons. IPSP/Cs could only rarely be elicited in spiny projection neurons (SPNs) in paired recordings with NPY-PLTS interneurons. In contrast, the probability of SPN innervation by NPY-neurogliaform interneurons was extremely high, the synapse very reliable (no failures were observed), and the resulting postsynaptic response was a slow, GABA(A) receptor-mediated IPSC that has not been previously described in striatum but that has been elicited from NPY-GABAergic neurogliaform interneurons in cortex and hippocampus. These properties suggest unique and distinctive roles for NPY-PLTS and NPY-neurogliaform interneurons in the integrative properties of the neostriatum.
authors.affiliation: Center for Molecular and Behavioral Neuroscience, Rutgers University, Newark, New Jersey 07102, USA.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Ibáñez-Sandoval
authors.forename: Fatuel
authors.initials: F
authors.lastname: Tecuapetla
authors.forename: Bengi
authors.initials: B
authors.lastname: Unal
authors.forename: Fulva
authors.initials: F
authors.lastname: Shah
authors.forename: Tibor
authors.initials: T
authors.lastname: Koós
authors.forename: James M
authors.initials: JM
authors.lastname: Tepper
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002452:Cell Count; D002540:Cerebral Cortex / Q000502:physiology; D003342:Corpus Striatum / Q000166:cytology*; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D018756:GABA Antagonists / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics; D066298:In Vitro Techniques; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects; D007395:Interneurons / Q000145:classification* / Q000502:physiology*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020932:Nerve Growth Factor / Q000378:metabolism; D009434:Neural Pathways / Q000502:physiology; D009478:Neuropeptide Y / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011810:Quinoxalines / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism
chemical_list: D018691:Excitatory Amino Acid Antagonists; D018756:GABA Antagonists; D009478:Neuropeptide Y; D011810:Quinoxalines; D049452:Green Fluorescent Proteins; D013004:Somatostatin; C056723:FG 9041; D020932:Nerve Growth Factor; D019001:Nitric Oxide Synthase; C013411:biocytin; D008239:Lysine; D001640:Bicuculline
references.citation: Neuron. 2008 Mar 27;57(6):917-29
references.pmid: 18367092
references.citation: Brain Res. 1990 Nov 26;534(1-2):111-6
references.pmid: 1705847
references.citation: J Neurosci. 2009 Jun 17;29(24):7776-87
references.pmid: 19535589
references.citation: Eur J Neurosci. 2002 Jun;15(12):2049-52
references.pmid: 12099911
references.citation: Brain Res. 1996 Oct 7;735(2):317-24
references.pmid: 8911672
references.citation: J Neurosci. 2009 Jul 15;29(28):8977-90
references.pmid: 19605635
references.citation: J Neurophysiol. 2009 Nov;102(5):3038-45
references.pmid: 19759327
references.citation: Neurosci Lett. 1989 May 22;100(1-3):99-104
references.pmid: 2761790
references.citation: J Comp Neurol. 2004 Feb 9;469(3):325-39
references.pmid: 14730585
references.citation: J Neurophysiol. 1989 Nov;62(5):1052-68
references.pmid: 2585039
references.citation: J Comp Neurol. 2010 Feb 1;518(3):389-404
references.pmid: 19950390
references.citation: J Neurophysiol. 2002 Sep;88(3):1263-9
references.pmid: 12205147
references.citation: Nat Neurosci. 1999 May;2(5):467-72
references.pmid: 10321252
references.citation: J Neurosci. 2003 Jul 16;23(15):6245-54
references.pmid: 12867509
references.citation: J Neurophysiol. 2007 Feb;97(2):1030-9
references.pmid: 17122314
references.citation: Trends Neurosci. 2000 Mar;23(3):120-6
references.pmid: 10675916
references.citation: J Neurophysiol. 1993 Jul;70(1):387-96
references.pmid: 8395585
references.citation: Brain Res Rev. 2008 Aug;58(2):272-81
references.pmid: 18054796
references.citation: Front Neuroanat. 2010 Dec 29;4:150
references.pmid: 21228905
references.citation: J Neurosci. 2010 Mar 3;30(9):3499-507
references.pmid: 20203210
references.citation: Trends Neurosci. 1995 Dec;18(12):527-35
references.pmid: 8638293
references.citation: Brain Res. 2001 Sep 21;913(2):117-32
references.pmid: 11549375
references.citation: J Neurosci. 2005 Oct 19;25(42):9773-81
references.pmid: 16237181
references.citation: Neurochem Res. 2008 Aug;33(8):1452-8
references.pmid: 18270823
references.citation: Nat Neurosci. 2011 Dec 11;15(1):123-30
references.pmid: 22158514
references.citation: Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14831-6
references.pmid: 17785408
references.citation: Front Syst Neurosci. 2011 Jun 06;5:41
references.pmid: 21713112
references.citation: Neuroscience. 2002;109(3):555-67
references.pmid: 11823066
references.citation: Mamm Genome. 2007 Oct;18(10):693-708
references.pmid: 17882484
references.citation: J Neurosci. 2010 May 19;30(20):6999-7016
references.pmid: 20484642
references.citation: Trends Neurosci. 2004 Nov;27(11):662-9
references.pmid: 15474166
references.citation: Adv Neurol. 1993;60:40-52
references.pmid: 8380527
references.citation: J Neurosci. 2009 Mar 18;29(11):3642-59
references.pmid: 19295167
references.citation: J Neurosci. 2010 Feb 10;30(6):2165-76
references.pmid: 20147544
references.citation: J Neurosci. 2010 Jul 21;30(29):9898-909
references.pmid: 20660272
references.citation: Science. 2003 Mar 21;299(5614):1902-5
references.pmid: 12649485
references.citation: Brain Res. 1985 Feb 18;327(1-2):307-11
references.pmid: 3986508
references.citation: Science. 2008 Jul 4;321(5885):53-7
references.pmid: 18599766
references.citation: Brain Res. 2000 Apr 28;863(1-2):182-91
references.pmid: 10773206
references.citation: J Neurosci. 2010 Feb 10;30(6):2223-34
references.pmid: 20147549
references.citation: J Neurosci. 2000 Jan 1;20(1):375-86
references.pmid: 10627614
references.citation: J Neurosci. 1993 Nov;13(11):4908-23
references.pmid: 7693897
references.citation: J Comp Neurol. 1995 Jan 23;351(4):499-508
references.pmid: 7721980
references.citation: J Neurosci. 2002 Jan 15;22(2):529-35
references.pmid: 11784799
references.citation: Nature. 2003 Oct 30;425(6961):917-25
references.pmid: 14586460
references.citation: Prog Brain Res. 1993;99:277-97
references.pmid: 8108553
references.citation: J Comp Neurol. 1996 Mar 18;366(4):580-99
references.pmid: 8833111
references.citation: J Neurosci. 2005 Jul 20;25(29):6775-86
references.pmid: 16033887
references.citation: J Neurosci. 2004 Sep 8;24(36):7916-22
references.pmid: 15356204
references.citation: Neuron. 1993 Feb;10(2):189-200
references.pmid: 8382497
references.citation: Hippocampus. 1996;6(4):347-470
references.pmid: 8915675
references.citation: J Neurosci. 1996 Apr 1;16(7):2397-410
references.pmid: 8601819
references.citation: Neuroscience. 2007 May 11;146(2):537-54
references.pmid: 17324523
references.citation: Curr Opin Neurobiol. 2004 Dec;14(6):685-92
references.pmid: 15582369
references.citation: J Comp Neurol. 1999 Feb 1;404(1):1-20
references.pmid: 9886021
references.citation: Brain Res. 2007 Jun 4;1152:49-56
references.pmid: 17459351