title: Paralemniscal TIP39 is induced in rat dams and may participate in maternal functions.
abstract: The paralemniscal area, situated between the pontine reticular formation and the lateral lemniscus in the pontomesencephalic tegmentum contains some tuberoinfundibular peptide of 39 residues (TIP39)-expressing neurons. In the present study, we measured a 4 times increase in the level of TIP39 mRNA in the paralemniscal area of lactating mothers as opposed to nulliparous females and mothers deprived of pups using real-time RT-PCR. In situ hybridization histochemistry and immunolabeling demonstrated that the induction of TIP39 in mothers takes place within the medial paralemniscal nucleus, a cytoarchitectonically distinct part of the paralemniscal area, and that the increase in TIP39 mRNA levels translates into elevated peptide levels in dams. The paralemniscal area has been implicated in maternal control as well as in pain perception. To establish the function of induced TIP39, we investigated the activation of TIP39 neurons in response to pup exposure as maternal, and formalin injection as noxious stimulus. Both stimuli elicited c-fos expression in the paralemniscal area. Subsequent double labeling demonstrated that 95% of neurons expressing Fos in response to pup exposure also contained TIP39 immunoreactivity and 91% of TIP39 neurons showed c-fos activation by pup exposure. In contrast, formalin-induced Fos does not co-localize with TIP39. Instead, most formalin-activated neurons are situated medial to the TIP39 cell group. Our data indicate that paralemniscal neurons may be involved in the processing of maternal and nociceptive information. However, two different groups of paralemniscal neurons participate in the two functions. In particular, TIP39 neurons may participate in the control of maternal functions.
authors.affiliation: Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Histology and Embryology, Semmelweis University and Hungarian Academy of Sciences, Tüzolto u. 58, Budapest, 1094, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Varga
authors.forename: Bence
authors.initials: B
authors.lastname: Mogyoródi
authors.forename: Attila G
authors.initials: AG
authors.lastname: Bagó
authors.forename: Melinda
authors.initials: M
authors.lastname: Cservenák
authors.forename: Dominika
authors.initials: D
authors.lastname: Domokos
authors.forename: Eva
authors.initials: E
authors.lastname: Renner
authors.forename: Katalin
authors.initials: K
authors.lastname: Gallatz
authors.forename: Ted B
authors.initials: TB
authors.lastname: Usdin
authors.forename: Miklós
authors.initials: M
authors.lastname: Palkovits
authors.forename: Arpád
authors.initials: A
authors.lastname: Dobolyi
mesh_terms: D000818:Animals; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D017403:In Situ Hybridization; D007774:Lactation / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D059225:Nociception / Q000502:physiology*; D011149:Pons / Q000378:metabolism* / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012154:Reticular Formation / Q000378:metabolism* / Q000473:pathology; D013681:Tegmentum Mesencephali / Q000378:metabolism* / Q000473:pathology
chemical_list: D009479:Neuropeptides; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C400136:tuberoinfundibular peptide 39; D005557:Formaldehyde
references.citation: Endocrinology. 2001 Aug;142(8):3451-6
references.pmid: 11459790
references.citation: J Comp Neurol. 1978 Dec 15;182(4 Pt 2):763-76
references.pmid: 730846
references.citation: Prog Brain Res. 2001;133:1-38
references.pmid: 11589124
references.citation: J Comp Neurol. 2003 Aug 18;463(2):132-56
references.pmid: 12815752
references.citation: Ann Ist Super Sanita. 2004;40(2):223-9
references.pmid: 15536274
references.citation: J Comp Neurol. 2010 Nov 1;518(21):4375-94
references.pmid: 20853513
references.citation: Neuroreport. 2004 Aug 6;15(11):1779-82
references.pmid: 15257146
references.citation: Neuroscience. 2003;122(4):1093-105
references.pmid: 14643775
references.citation: Neuroscience. 2001;104(1):181-97
references.pmid: 11311541
references.citation: Ann N Y Acad Sci. 2004 Jun;1018:16-24
references.pmid: 15240348
references.citation: Nat Neurosci. 1999 Nov;2(11):941-3
references.pmid: 10526330
references.citation: Psychoneuroendocrinology. 1996 Feb;21(2):203-17
references.pmid: 8774063
references.citation: Neuroscience. 1994 Jan;58(2):287-98
references.pmid: 8152540
references.citation: Neuroscience. 1988 Jun;25(3):997-1005
references.pmid: 3405439
references.citation: J Comp Neurol. 2006 Sep 20;498(3):375-89
references.pmid: 16871538
references.citation: Exp Anim. 2001 Jul;50(4):307-12
references.pmid: 11515093
references.citation: Neuroscience. 2008 Jun 2;153(4):1020-33
references.pmid: 18440151
references.citation: Stress. 1997 May;1(3):155-168
references.pmid: 9787241
references.citation: Endocrinology. 2003 Jul;144(7):2791-6
references.pmid: 12810532
references.citation: Horm Behav. 1979 Feb;12(1):95-102
references.pmid: 478446
references.citation: J Comp Neurol. 1996 Apr 29;368(2):252-69
references.pmid: 8725305
references.citation: Exp Brain Res. 2006 Feb;169(1):92-105
references.pmid: 16292643
references.citation: Prog Neurobiol. 2010 Jan 11;90(1):29-59
references.pmid: 19857544
references.citation: Brain Struct Funct. 2009 Dec;214(1):15-23
references.pmid: 19936783
references.citation: Brain Res. 1989 Apr 3;483(2):272-82
references.pmid: 2706520
references.citation: Annu Rev Neurosci. 1991;14:421-51
references.pmid: 1903243
references.citation: J Neurosci Methods. 1980 Dec;3(2):129-49
references.pmid: 6110810
references.citation: Brain Res Brain Res Rev. 1998 Dec;28(3):370-490
references.pmid: 9858769
references.citation: J Comp Neurol. 2003 Jan 20;455(4):547-66
references.pmid: 12508326
references.citation: Exp Neurol. 2010 Nov;226(1):68-83
references.pmid: 20696160
references.citation: Prog Neurobiol. 2006 Oct;80(2):53-83
references.pmid: 17030082
references.citation: Anat Embryol (Berl). 1997 Sep;196(3):235-59
references.pmid: 9310315
references.citation: J Comp Neurol. 2008 Nov 10;511(2):221-37
references.pmid: 18770870
references.citation: Am J Physiol. 1998 Oct;275(4):R1247-55
references.pmid: 9756557
references.citation: J Neurosci. 1993 May;13(5):1899-915
references.pmid: 8478683
references.citation: Can J Med Technol. 1965 Aug;27:118-23
references.pmid: 14341644
references.citation: Trends Pharmacol Sci. 2000 Apr;21(4):128-30
references.pmid: 10740285
references.citation: Neuroscience. 1999;94(1):117-29
references.pmid: 10613502
references.citation: J Neurosci. 2006 Jun 28;26(26):7105-15
references.pmid: 16807339
references.citation: Endocrinology. 2010 Dec;151(12):5830-40
references.pmid: 20861230
references.citation: Brain Res. 2010 Jan 8;1306:29-38
references.pmid: 19833107
references.citation: J Comp Neurol. 1990 Jun 22;296(4):517-30
references.pmid: 2113539
references.citation: Brain Res. 1983 May 16;267(2):217-23
references.pmid: 6307465
references.citation: Eur J Neurosci. 2002 Nov;16(10):1974-86
references.pmid: 12453061
references.citation: Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1651-6
references.pmid: 11818570
references.citation: J Comp Neurol. 1997 Jul 14;383(4):439-58
references.pmid: 9208992
references.citation: Brain Res. 1999 Apr 10;824(2):267-76
references.pmid: 10196458
references.citation: J Comp Neurol. 1999 Mar 15;405(3):359-79
references.pmid: 10076931
references.citation: J Comp Neurol. 1990 Jan 22;291(4):553-82
references.pmid: 2329191