title: [In vivo and in vitro effect of peptide HP-6 derived from donkey serum albumin on hematopoietic system].
abstract: OBJECTIVE
  By bioinformatics method, the effect in hematopoietic system of bioactive peptide HP-6, which was obtained from donkey serum albumin and is one of the major protein components from donkey-hide gelatin, was investigated.
  
  
  METHODS
  Human bone marrow nucleated cells (hBMNCs) and murine bone marrow stromal cells (mBMSCs) were separated and cultured with different concentration of peptide HP-6 (0.000 15, 0.001 5, 0.015, 0.15, 1.5 micromol x L(-1)). The effect on promoting proliferation of cells related to hematopoiesis in bone morrow was detected and the ultrastructure of cells after treated by HP-6 was observed through transmission electron microscope. Hemorrhage anemia mouse model and anemia mouse model induced by cyclophosphamide were established, and randomly divided into peptide HP-6 groups which were administered respectively with different doses (1, 0.1, 0.01 mg x kg(-1)) by gavage, and control group which was administered with PBS by gavage. Peripheral blood components of all mice and bone morrow cells (BMC) number of mice induced by cyclophosphamide were evaluated.
  
  
  RESULTS
  Peptide HP-6 could concentration-related promote the proliferation of hBMNCs and mBMSCs, hBMNCs got the highest reproduction rate of 152.11% and mBMSCs also got 63.52% with the concentration of 0.15 micromol x L(-1), then the reproduction rate decreased while the concentration kept increasing. The transmission electron microscope showed that ultrastructure of cells was normal after treated by HP-6.1 mg x kg(-1) peptide HP-6 significantly increased peripheral platelet and protected mouse morrow injured by cyclophoshamide. 0.1 mg x kg(-1) peptide HP-6 significantly increased peripheral platelet with relative growth rate of 77.65%, increased peripheral white blood cells count and peripheral red blood cells count, also could protect mouse peripheral blood after treated by chemotherapeutics.
  
  
  CONCLUSIONS
  Peptide HP-6 could promote the proliferation of cells related to hematopoietic system, enhance mouse hemopoiesis function and the resistance to chemotherapeutic injury.
authors.affiliation: Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China. lihao777@126.com
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Shengmin
authors.initials: S
authors.lastname: Yan
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaomiao
authors.initials: X
authors.lastname: Shen
authors.forename: Pingfan
authors.initials: P
authors.lastname: Rao
mesh_terms: D000740:Anemia / Q000188:drug therapy; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D018397:Equidae / Q000097:blood*; D005260:Female; D005780:Gelatin / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects; D006413:Hematopoietic System / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D012709:Serum Albumin / Q000494:pharmacology*
chemical_list: D010455:Peptides; D012709:Serum Albumin; D005780:Gelatin