title: [High-throughput screening of human pancreatic alpha-amylase inhibitors].
abstract: OBJECTIVE
  Targeting the important enzyme in human glucose metabolic pathway, we established a high throughput screening model for human pancreatic alpha-amylase inhibitors.
  
  
  METHODS
  Pichia pastoris expression system was used to clone and express the human pancreatic alpha-amylase; we established the alpha-amylase inhibitor screening model using the catalytic properties of enzyme; this model was applied in screening of actinomycete' metabolites; the taxonomic status of positive strains were analyzed by constructing 16S rRNA phylogenetic tree.
  
  
  RESULTS
  We cloned and expressed the intact gene of human pancreatic alpha-amylase successfully; the high-throughput screening model of alpha-amylase inhibitors was established; nearly 2000 actinomycete' metabolites were screened, 14 alpha-amylase inhibitor producing strains were obtained finally, and showed taxonomically rich diversity.
  
  
  CONCLUSIONS
  The alpha-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs.
authors.affiliation: College of Pharmacy, Nankai University, Tianjin 300071, China. qixizhen@163.com
authors.forename: Xizhen
authors.initials: X
authors.lastname: Qi
authors.forename: Limei
authors.initials: L
authors.lastname: Ren
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Bai
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D057166:High-Throughput Screening Assays; D006801:Humans; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors* / Q000235:genetics; D010802:Phylogeny; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D055292:Pancreatic alpha-Amylases