title: Sequential microfluidic droplet processing for rapid DNA extraction.
abstract: This work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1 min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for λ-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P R China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pan
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Zeng
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D001435:Bacteriophages / Q000737:chemistry; D004279:DNA, Viral / Q000302:isolation & purification*; D004867:Equipment Design; D060526:Magnetic Fields; D059346:Magnets; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral