title: Identification of genes up-regulated during somatic embryogenesis of cucumber.
abstract: Somatic embryogenesis is a method of plant regeneration, but it can also be used as a model to study plant development. A normalized library of cDNA fragments representing genes up-regulated after the induction of somatic embryogenesis in cucumber suspension cultures was constructed using the suppression subtractive hybridization technique. Candidate cDNA fragments (119) were classified according to their similarity to genes encoding known proteins and the presence of potential functional domains. Of the translation products with homology to known proteins, about 23% were possibly involved in metabolism, 13% represented proteins with a probable role in cellular communication and signal transduction, about 12% were likely to participate in protein synthesis, while around 10% were potential transcription factors. The genes corresponding to four of the cDNAs were subsequently analyzed in more detail: CsSEF2, CsSEM1 and CsSESTK1 encoding putative transcription factors or co-activators, and CsSECAD1 encoding cinnamyl alcohol dehydrogenase. Full-length cDNAs were isolated and analyzed. RT-PCR confirmed the up-regulation of these genes after the induction of somatic embryogenesis and showed the presence of their transcripts in other tissues. The in situ localization of transcripts of the CsSEF2 and CsSEM1 genes demonstrated that signalling in somatic embryo tissues involving these factors is concentrated in the cotyledon primordia and roots.
authors.affiliation: Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland. anita_wisniewska@sggw.pl
authors.forename: Anita
authors.initials: A
authors.lastname: Wiśniewska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grabowska
authors.forename: Anna
authors.initials: A
authors.lastname: Pietraszewska-Bogiel
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tagashira
authors.forename: Sabina
authors.initials: S
authors.lastname: Zuzga
authors.forename: Rafał
authors.initials: R
authors.lastname: Wóycicki
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Przybecki
authors.forename: Stefan
authors.initials: S
authors.lastname: Malepszy
authors.forename: Marcin
authors.initials: M
authors.lastname: Filipecki
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D018548:Cotyledon / Q000378:metabolism; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D018076:DNA, Complementary; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D008660:Metabolism / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D014176:Protein Biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017385:Sequence Homology; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C018656:cinnamyl alcohol dehydrogenase