title: Effect of β -catenin siRNA on proliferation and apoptosis of hepatoma cell line SMMC-7721 and HepG-2.
abstract: OBJECTIVE
  The aim of this study was to investigate the effect of expression of siRNA β- catenin silent gene on cell cycle and apoptosis of different hepatoma cells.
  
  
  METHODS
  The effect of the β-catenin-siRNA recombinant plasmid on the growth of different hepatoma cells was observed by MTT assays; and the effect of the recombinant plasmid on proliferation and apoptosis of different hepatoma cells was detected through flow cytometry.
  
  
  RESULTS
  Transfection of the β-catenin-siRNA recombinant plasmid into hepatoma cells significantly inhibited β-catenin expression at protein and mRNA levels (p<0.05) as shown by western blotting and RT-PCR, respectively. The MTT assay showed that β-catenin-siRNA can significantly inhibit growth of hepatoma cells. The results of flow cytometry showed that the growth of different hepatoma cells was blocked at G0/G1 phase and compared with the control group there was a significant difference (p<0.05). Flow cytometry also showed that apoptosis of different hepatoma cells, compared with the control group, was significantly different (p<0.05).
  
  
  CONCLUSIONS
  Inhibition of β-catenin in hepatoma cells can inhibit growth and promote apoptosis, and there was difference in the effect on proliferation and apoptosis between different hepatoma cells.
authors.affiliation: Department of Gastroenterology, Jilin University, Jilin, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Liyan
authors.forename: Sun
authors.initials: S
authors.lastname: Xun
authors.forename: Meng
authors.initials: M
authors.lastname: Xiangwei
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000628:therapy*; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000628:therapy*; D018613:Microscopy, Confocal; D034741:RNA, Small Interfering / Q000235:genetics* / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D051176:beta Catenin / Q000235:genetics* / Q000494:pharmacology*
chemical_list: D034741:RNA, Small Interfering; D051176:beta Catenin