title: Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein, MolA.
abstract: molA (HI1472) from H. influenzae encodes a periplasmic binding protein (PBP) that delivers substrate to the ABC transporter MolB(2)C(2) (formerly HI1470/71). The structures of MolA with molybdate and tungstate in the binding pocket were solved to 1.6 and 1.7 Å resolution, respectively. The MolA-binding protein binds molybdate and tungstate, but not other oxyanions such as sulfate and phosphate, making it the first class III molybdate-binding protein structurally solved. The ∼100 μM binding affinity for tungstate and molybdate is significantly lower than observed for the class II ModA molybdate-binding proteins that have nanomolar to low micromolar affinity for molybdate. The presence of two molybdate loci in H. influenzae suggests multiple transport systems for one substrate, with molABC constituting a low-affinity molybdate locus.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Leidamarie
authors.initials: L
authors.lastname: Tirado-Lee
authors.forename: Allen
authors.initials: A
authors.lastname: Lee
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Rees
authors.forename: Heather W
authors.initials: HW
authors.lastname: Pinkett
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry* / Q000145:classification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006193:Haemophilus influenzae; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008982:Molybdenum / Q000737:chemistry; D033903:Periplasmic Proteins / Q000737:chemistry* / Q000145:classification; D017434:Protein Structure, Tertiary; D013816:Thermodynamics; D017186:Titrimetry; D017973:Tungsten Compounds / Q000737:chemistry
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D033903:Periplasmic Proteins; D017973:Tungsten Compounds; D008982:Molybdenum; C045951:tungstate
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