title: A prototypic system of parallel electrophoresis in multiple capillaries coupled with microwell arrays.
abstract: We present a microfluidic system that can be directly coupled with microwell array and perform parallel electrophoresis in multiple capillaries simultaneously. The system is based on an array of glass capillaries, fixed in a polydimethylsiloxane (PDMS) microfluidic scaffold, with one end open for interfacing with microwells. In this capillary array, every two adjacent capillaries act as a pair to be coupled with one microwell; samples in the microwells are introduced and separated by simply applying voltage between two electrodes that are placed at the other ends of capillaries; thus no complicated circuit design is required. We evaluate the performance of this system and perform multiple CE with direct sample introduction from microwell array. Also with this system, we demonstrate the analysis of cellular contents of cells lysed in a microwell array. Our results show that this prototypic system is a promising platform for high-throughput analysis of samples in microwell arrays.
authors.affiliation: Nano Science and Technology Program, Hong Kong University of Science and Technology, Kowloon, Hong Kong, P R China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Kangning
authors.initials: K
authors.lastname: Ren
authors.forename: Wen
authors.initials: W
authors.lastname: Dai
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhou
authors.forename: Hongkai
authors.initials: H
authors.lastname: Wu
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon