title: Effect of double growth factor release on cartilage tissue engineering.
abstract: The effects of double release of insulin-like growth factor I (IGF-I) and growth factor β1 (TGF-β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-β1, respectively. On tissue culture polystyrene (TCPS), TGF-β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400 µm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-β1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue.
authors.affiliation: Department of Genetics and Bioengineering, Yeditepe University, Faculty of Engineering and Architecture, Istanbul, Turkey.
authors.forename: Ayşe Burcu
authors.initials: AB
authors.lastname: Ertan
authors.forename: Pınar
authors.initials: P
authors.lastname: Yılgor
authors.forename: Banu
authors.initials: B
authors.lastname: Bayyurt
authors.forename: Ayşe Ceren
authors.initials: AC
authors.lastname: Calıkoğlu
authors.forename: Ciğdem
authors.initials: C
authors.lastname: Kaspar
authors.forename: Fatma Neşe
authors.initials: FN
authors.lastname: Kök
authors.forename: Gamze Torun
authors.initials: GT
authors.lastname: Kose
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
mesh_terms: D000178:Acrylamides / Q000494:pharmacology; D000180:Acrylic Resins; D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D002356:Cartilage / Q000187:drug effects*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology; D008297:Male; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012710:Serum Albumin, Bovine / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D053674:Aggrecans; D024043:Collagen Type II; D006025:Glycosaminoglycans; D011108:Polymers; D053773:Transforming Growth Factor beta1; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C052970:poly-N-isopropylacrylamide; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D007334:Insulin-Like Growth Factor I; D003094:Collagen