title: Genes involved in glucose repression and oxidative  stress response in the fission yeast Schizosaccharomyces pombe.
abstract: We looked for changes in gene expression and novel  genes that could be involved in the interaction between glucose repression and  oxidative stress response in the fission yeast, Schizosaccharomyces pombe,  using a constitutive invertase mutant, ird11, which is resistant to  glucose. BLAST analysis was made of the S. pombe genome database of  cDNAs whose expression ratios differentially decreased or increased upon  exposure to mild oxidative stress in this mutant compared to the wild type.  Genes with this type of activity were identified as rpl302, encoding 60S  ribosomal protein L3, and mpg1, encoding mannose-1-phosphate  guanyltransferase; their expression patterns were measured using quantitative  real-time PCR. We found that the expression levels of rpl302 and mpg1 genes in ird11 under unstressed conditions were increased compared  to those of the wild type. Under stress conditions, the expression levels of  the rpl302 gene were decreased in both strains, while mpg1 expression  levels remained unchanged. These results suggest that these genes play a role  in the response to oxidative stress in this mutant strain.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, Turkey.
authors.forename: K G
authors.initials: KG
authors.lastname: Suslu
authors.forename: B
authors.initials: B
authors.lastname: Palabiyik
authors.forename: G
authors.initials: G
authors.lastname: Temizkan
mesh_terms: D018076:DNA, Complementary / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D005947:Glucose / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D029702:Schizosaccharomyces pombe Proteins; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D005947:Glucose