title: CD133+ liver cancer stem cells modulate radioresistance in human hepatocellular carcinoma.
abstract: CD133 is a cancer stem-cell (CSC) marker associated with radioresistance and chemoresistance in various cancers. In the present study, CD133-expressing liver cancer cells following radiation exposure showed higher activation of MAPK/PI3K signaling pathway and reduction in reactive oxygen species levels compared to CD133- cells. The in vivo study with a xenograft model showed increased tumor formation in irradiated CD133+ cell-injected nude mice compared to the CD133- group, suggesting that CD133 contributes to radioresistance in HCC. Therefore, CD133-expressing liver cancer cells have anti-apoptotic and radioresistance properties that may be useful to improve anti-cancer treatments, including chemotherapy/radiotherapy of HCC.
authors.affiliation: WHO Collaborating Center of Viral Hepatitis, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Lian Shu
authors.initials: LS
authors.lastname: Piao
authors.forename: Wonhee
authors.initials: W
authors.lastname: Hur
authors.forename: Taek-Kyun
authors.initials: TK
authors.lastname: Kim
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Hong
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Choi
authors.forename: Pil Soo
authors.initials: PS
authors.lastname: Sung
authors.forename: Myeong Joon
authors.initials: MJ
authors.lastname: Song
authors.forename: Byeong-Chel
authors.initials: BC
authors.lastname: Lee
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Seung Kew
authors.initials: SK
authors.lastname: Yoon
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism*; D017209:Apoptosis / Q000528:radiation effects; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000503:physiopathology*; D002453:Cell Cycle / Q000528:radiation effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046228:Microarray Analysis; D014411:Neoplastic Stem Cells / Q000473:pathology* / Q000528:radiation effects / Q000637:transplantation; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D011836:Radiation Tolerance / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D017382:Reactive Oxygen Species