title: Virus-binding activity of the truncated C subunit of porcine aminopeptidase N expressed in Escherichia coli.
abstract: Seven overlapping truncated forms of the C subunit of porcine aminopeptidase N (pAPN-C) were expressed in Escherichia coli. By western blotting and ELISA test, all recombinant proteins were recognized by the antibody against native porcine aminopeptidase N. Recombinant proteins, rpAPN-C2 (aa 623-722) and rpAPN-C3 (aa 673-772), had the highest binding activity with swine transmissible gastroenteritis virus among the truncated pAPN-C recombinant proteins. The overlapping region (aa 673-722) between rpAPN-C2 and rpAPN-C3 is indicated to play a key role in viral binding.
authors.affiliation: Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Nangang District, Harbin 150001, People's Republic of China. dongbosun@yahoo.com.cn
authors.forename: Dongbo
authors.initials: D
authors.lastname: Sun
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shi
authors.forename: Jianfei
authors.initials: J
authors.lastname: Chen
authors.forename: Donghua
authors.initials: D
authors.lastname: Guo
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
authors.forename: Xianjing
authors.initials: X
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Huaji
authors.initials: H
authors.lastname: Qiu
authors.forename: Li
authors.initials: L
authors.lastname: Feng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000502:physiology*; D053585:Virus Attachment
chemical_list: D011991:Receptors, Virus; D011994:Recombinant Proteins; D018826:CD13 Antigens
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