title: Use of a commercial PCR-based line blot method for identification of bacterial pathogens and the mecA and van genes from BacTAlert blood culture bottles.
abstract: In this study, the PCR-based DNA strip assay GenoType BC for the identification of bacteria and the resistance genes mecA, vanA, vanB, vanC1, and vanC2/3 directly from positive BacTAlert blood culture bottles was evaluated in a multicenter study. Of a total of 511 positive blood cultures, correct identification percentages for Gram-negative bacteria, Gram-positive bacteria, and the mecA gene were 96.1%, 89.9%, and 92.9%, respectively. Results were available 4 h after growth detection.
authors.affiliation: Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Mathis
authors.initials: M
authors.lastname: Steindor
authors.forename: Michael
authors.initials: M
authors.lastname: Weizenegger
authors.forename: Nicole
authors.initials: N
authors.lastname: Harrison
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Hirschl
authors.forename: Birgitta
authors.initials: B
authors.lastname: Schweickert
authors.forename: Ulf B
authors.initials: UB
authors.lastname: Göbel
authors.forename: Colin R
authors.initials: CR
authors.lastname: Mackenzie
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors; D020713:Vancomycin Resistance
chemical_list: D004269:DNA, Bacterial
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