title: The chloroplast ATP-dependent Clp protease in vascular plants - new dimensions and future challenges.
abstract: The ATP-dependent Clp protease is by far the most intricate protease in chloroplasts of vascular plants. Structurally, it is particularly complex with a proteolytic core complex containing 11 distinct subunits along with three potential chaperone partners. The Clp protease is also essential for chloroplast development and overall plant viability. Over the past decade, many of the important characteristics of this crucial protease have been revealed in the model plant species Arabidopsis thaliana. Despite this, challenges still remain in fully resolving certain key features, in particular, how the assembly of this multisubunit protease is regulated, the full range of native protein substrates and how they are targeted for degradation and how this complicated enzyme might have developed from simpler bacterial forms. This article focuses upon the recent advances in revealing the details underlying these important features. It also take the opportunity to speculate upon many of these findings in the hope of stimulating further investigation.
authors.affiliation: Department of Plant and Environmental Sciences, Gothenburg University, 40530 Gothenburg, Sweden. adrian.clarke@dpes.gu.se
authors.forename: Adrian K
authors.initials: AK
authors.lastname: Clarke
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D002470:Cell Survival; D060365:Chloroplast Proteins / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D049071:Endopeptidase Clp / Q000378:metabolism*; D004789:Enzyme Activation; D059828:Plant Cells / Q000201:enzymology; D018521:Plant Physiological Phenomena; D059748:Proteolysis; D013379:Substrate Specificity
chemical_list: D060365:Chloroplast Proteins; D049071:Endopeptidase Clp