title: The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells.
abstract: The candidate tumor suppressor fragile histidine traid (FHIT) is frequently inactivated in small cell lung cancer (SCLC). Mutations in the p53 gene also occur in the majority of SCLC leading to the accumulation of the mutant protein. Here we evaluated the effect of FHIT gene therapy alone or in combination with the mutant p53-reactivating molecule, PRIMA-1(Met)/APR-246, in SCLC. Overexpression of FHIT by recombinant adenoviral vector (Ad-FHIT)-mediated gene transfer in SCLC cells inhibited their growth by inducing apoptosis and when combined with PRIMA-1(Met)/APR-246, a synergistic cell growth inhibition was achieved.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The University of Texas, M.D. Anderson Cancer Center, Houston, USA. roza@rh.dk
authors.forename: Roza
authors.initials: R
authors.lastname: Zandi
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.forename: Hans S
authors.initials: HS
authors.lastname: Poulsen
authors.forename: Jack A
authors.initials: JA
authors.lastname: Roth
authors.forename: Lin
authors.initials: L
authors.lastname: Ji
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D018288:Carcinoma, Small Cell / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D015870:Gene Expression; D015316:Genetic Therapy; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy*; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D000099170:Bis(5'-adenosyl)triphosphatase; C495901:TP53 protein, human
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