title: Suppression of murine melanoma growth by a vaccine of attenuated Salmonella             carrying heat shock protein 70 and Herpes simplex virus-thymidine kinase genes.
abstract: Attenuated Salmonella can invade tumor cells and acts as a eukaryotic expression             vector for gene propagation. We constructed a bi-gene, eukaryotic co-expression             DNA vaccine of Mycobacterium tuberculosis heat shock protein 70 (mtHSP70) and             Herpes simplex virus-thymidine kinase (HSV-tk) and used attenuated Salmonella             as a vector to treat murine melanoma. In vitro, recombinant Salmonella can carry             plasmid stably and can invade into the cytoplasm of B16 tumor cells expressing             the protein of the mtHSP70/HSV-tk gene by Western blot assay. In vivo, after the             recombinant Salmonella was injected into tumors, the HSV-tk precursor drug ganciclovir             (GCV) was administered to start the HSV-tk killing of tumor cells. We found that             the mtHSP70/HSV-tk recombinant bacteria can raise CD8+ T lymphocytes in peripheral             blood by flow cytometry and in tumor tissues by immunofluorescence detection,             increase IFN‑γ contents in tumor tissue by ELISA and significantly suppress tumor             growth.
authors.affiliation: Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, PR China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zeng
authors.forename: Jincai
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiren
authors.initials: J
authors.lastname: Zhang
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.forename: Suwen
authors.initials: S
authors.lastname: Wang
authors.forename: Shiqing
authors.initials: S
authors.lastname: Wu
authors.forename: Ximao
authors.initials: X
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Wenge
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015774:Ganciclovir / Q000008:administration & dosage; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008546:Melanoma, Experimental / Q000382:microbiology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D012480:Salmonella Infections / Q000235:genetics; D022562:Salmonella Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics / Q000276:immunology*; D014162:Transfection / Q000379:methods; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D019496:Cancer Vaccines; D018840:HSP70 Heat-Shock Proteins; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D014764:Viral Proteins; D007371:Interferon-gamma; D013937:Thymidine Kinase; D015774:Ganciclovir