title: Proteomics analysis of aromatic catabolic pathways in thermophilic Geobacillus thermodenitrificans NG80-2.
abstract: Geobacillus thermodenitrificans NG80-2 is a crude oil-degrading thermophilic bacterium isolated from an oil reservoir in China. In this study, the gene clusters and pathways for the degradation of benzoate (via benzoyl-CoA), phenylacetate (via phenylacetyl-CoA), 4-hydroxyphenylacetate (via 3,4-dihydroxyphenylacetate) and anthranilate (via 3-hydroxyanthranilate) were confirmed using combined in silico analysis and proteomics approaches. It was observed that synthesis of the enzymes catalyzing initial activation, ring oxidation and ring cleavage reactions were generally induced specifically by their respective substrates, while many of the enzymes catalyzing downstream reactions exhibited broader substrate specificities. Novel genes encoding benzoyl-CoA epoxidase and 3,4-dihydroxyphenylacetate 2,3-dioxygenase, and a paaX homologue that serves as a positive regulator of benzoate degradation were proposed. Downregulation of the glycolysis pathway, along with upregulation of the gluconeogenesis pathway and the glyoxylate bypass (phenylacetate) were detected in association with the utilization of the aromatics. This novel proteomics approach confirmed the presence of multiple metabolic pathways for aromatic compounds in NG80-2, which is highly advantageous to the survival of this thermophilic bacterium under reservoir conditions.
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Li
authors.forename: Junli
authors.initials: J
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Ding
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001565:Benzoates / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D016678:Genome; D056506:Geobacillus / Q000378:metabolism*; D005943:Gluconeogenesis; D006019:Glycolysis; D007091:Image Processing, Computer-Assisted; D013058:Mass Spectrometry / Q000379:methods; D008660:Metabolism; D008956:Models, Chemical; D005810:Multigene Family; D010648:Phenylacetates / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001565:Benzoates; D010648:Phenylacetates