title: Cloning and characterization of a novel cold-active endoglucanase establishing a new subfamily of glycosyl hydrolase family 5 from a psychrophilic deep-sea bacterium.
abstract: The gene of a novel endo-β-1,4-glucanase (named Cel5M) was isolated from the psychrophilic deep-sea bacteria Pseudomonas sp. MM15. The deduced protein sequence lacked the typical cellulase domain structures of the carbohydrate-binding module and the linker region. Cel5M showed relatively higher activity toward carboxymethyl cellulose, but much lower activity toward p-nitrophenyl-β-D-galactopyranoside and no activity toward avicel. Cel5M was identified as a cold-active cellulase with an optimal temperature of 30 °C and it was active within a narrow pH range with an optimum at pH 4.5. Phylogenetic analysis showed that Cel5M represented a new subfamily of the glycosyl hydrolase family 5, representing an opportunity for research into and applications of novel cold-active cellulases.
authors.affiliation: State Key Laboratory of Heavy Oil Processing & Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Yang
authors.forename: Hongyue
authors.initials: H
authors.lastname: Dang
mesh_terms: D000595:Amino Acid Sequence; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D003001:Cloning, Molecular; D016000:Cluster Analysis; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D019015:Geologic Sediments / Q000382:microbiology*; D044643:Glucan 1,4-beta-Glucosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009598:Nitrophenylgalactosides / Q000378:metabolism; D010802:Phylogeny; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D009598:Nitrophenylgalactosides; D044643:Glucan 1,4-beta-Glucosidase; D002266:Carboxymethylcellulose Sodium