title: Isolation and identification of native membrane glycoproteins from living cell by concanavalin A-magnetic particle conjugates.
abstract: The discovery, isolation, and subsequent identification of cell membrane glycoproteins involved in the structure and function of the cell surface are becoming more and more important. Here, concanavalin A-magnetic particle conjugates were employed to isolate the special membrane glycoproteins from living HepG-2 cells. The isolated glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry as well as annotated. A total of 37 membrane glycoproteins were identified, and 25 of them were ascertained to locate in the extracellular region.
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, 710069 Xi'an, People's Republic of China.
authors.forename: Ganglong
authors.initials: G
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Cui
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Chen
authors.forename: Tianran
authors.initials: T
authors.lastname: Ma
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D002853:Chromatography, Liquid; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D056945:Hep G2 Cells; D006801:Humans; D058185:Magnetite Nanoparticles; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D053719:Tandem Mass Spectrometry
chemical_list: D058185:Magnetite Nanoparticles; D008562:Membrane Glycoproteins; D003208:Concanavalin A