title: Genistein reduces heparan sulfate accumulation in human mucolipidosis II skin fibroblasts.
abstract: Genistein, a soy isoflavone, reduces glycosaminoglycan synthesis and its effect on mucopolysaccharidoses has been tested. In this report, we examined the effect of genistein in human mucolipidosis II skin fibroblasts in vitro. Heparan sulfate was accumulated within both cells and in extracellular spaces in mucolipidosis II. Genistein reduced the amount of heparan sulfate in cultured cells dose dependently and also inhibited cell growth dose dependently.
authors.affiliation: Department of Pediatrics, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan. otomo@ped.med.osaka-u.ac.jp
authors.forename: Takanobu
authors.initials: T
authors.lastname: Otomo
authors.forename: Mohammad Arif
authors.initials: MA
authors.lastname: Hossain
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ozono
authors.forename: Norio
authors.initials: N
authors.lastname: Sakai
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D019833:Genistein / Q000494:pharmacology*; D006497:Heparan Sulfate / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D002479:Inclusion Bodies / Q000187:drug effects / Q000378:metabolism; D009081:Mucolipidoses / Q000378:metabolism*; D012867:Skin / Q000378:metabolism
chemical_list: D000888:Antibodies, Anti-Idiotypic; D019833:Genistein; D006497:Heparan Sulfate