title: MiR-25 regulates apoptosis by targeting Bim in human ovarian cancer.
abstract: MicroRNAs (miRNAs) are emerging as a class of small regulatory RNAs whose             alterations are implicated in the initiation and progression of human cancers.             Our study showed that miR-25 was highly expressed both in clinical ovarian cancer             samples and cell lines. Down-regulation of miR-25 in ovarian cancer cells induced             apoptosis whereas overexpression of miR-25 enhanced cell proliferation. The effects             of miR-25 abrogation were partly mediated by the intrinsic apoptosis pathway.             Many pro-apoptotic proteins such as Bim, Bax and caspase-3 were up-regulated after             transfection. Furthermore, luciferase assays demonstrated that Bim was the direct             target of miR-25. Introducing Bim cDNA without 3'UTR abrogated miR-25-induced             cell survival. Finally, there was an inverse relationship between Bim and miR-25             expression in ovarian cancer tissues. Taken together, these data indicate that             miR-25 directly regulates apoptosis by targeting Bim in ovarian cancer and that             miR-25 could be a potential therapeutic target for ovarian cancer intervention.
authors.affiliation: Obstetrics and Gynecology Hospital of Fudan University, No. 419, Fangxie Road, Shanghai, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zuo
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D000072224:Bcl-2-Like Protein 11; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins; C000606775:BCL2L11 protein, human; D000072224:Bcl-2-Like Protein 11; C543351:MIRN25 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins