title: Structural map of a microRNA-122: hepatitis C virus complex.
abstract: MicroRNA-122 (miR-122) enhances hepatitis C virus (HCV) fitness via targeting two sites in the 5'-untranslated region (UTR) of HCV. We used selective 2'-hydroxyl acylation analyzed by primer extension to resolve the HCV 5'-UTR's RNA secondary structure in the presence of miR-122. Nearly all nucleotides in miR-122 are involved in targeting the second site, beyond classic seed base pairings. These additional interactions enhance HCV replication in cell culture. To our knowledge, this is the first biophysical study of this complex to reveal the importance of 'tail' miR-122 nucleotide interactions.
authors.affiliation: Department of Medicinea and Protein and Nucleic Acid Facility, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Phillip S
authors.initials: PS
authors.lastname: Pang
authors.forename: Edward A
authors.initials: EA
authors.lastname: Pham
authors.forename: Menashe
authors.initials: M
authors.lastname: Elazar
authors.forename: Shripa G
authors.initials: SG
authors.lastname: Patel
authors.forename: Michael R
authors.initials: MR
authors.lastname: Eckart
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Glenn
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D016174:Hepacivirus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D006526:Hepatitis C / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D012367:RNA, Viral
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