title: Inhibition of microRNA-29 enhances elastin levels in cells haploinsufficient for elastin and in bioengineered vessels--brief report.
abstract: OBJECTIVE
  The goal of this study was to determine whether antagonizing microRNA (miR)-29 enhances elastin (ELN) levels in cells and tissues lacking ELN.
  
  
  RESULTS
  miR-29 mimics reduced ELN levels in fibroblasts and smooth muscle cells, whereas miR-29 inhibition increased ELN levels. Antagonism of miR-29 also increased ELN levels in cells from patients haploinsufficient for ELN and in bioengineered human vessels.
  
  
  CONCLUSIONS
  miR-29 antagonism may promote increased ELN levels during conditions of ELN deficiencies.
authors.affiliation: Department of Pharmacology, Yale University School of Medicine, Amistad Research Bldg, 10 Amistad St, New Haven, CT 06520, USA.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Angela
authors.initials: A
authors.lastname: Huang
authors.forename: Jacopo
authors.initials: J
authors.lastname: Ferruzzi
authors.forename: Robert P
authors.initials: RP
authors.lastname: Mecham
authors.forename: Barry C
authors.initials: BC
authors.lastname: Starcher
authors.forename: George
authors.initials: G
authors.lastname: Tellides
authors.forename: Jay D
authors.initials: JD
authors.lastname: Humphrey
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Giordano
authors.forename: Laura E
authors.initials: LE
authors.lastname: Niklason
authors.forename: William C
authors.initials: WC
authors.lastname: Sessa
mesh_terms: D021921:Aortic Stenosis, Supravalvular / Q000235:genetics / Q000378:metabolism; D001158:Arteries / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D003187:Compliance; D004549:Elastin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D057895:Haploinsufficiency; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D034622:RNA Interference; D023822:Tissue Engineering; D014162:Transfection; D015854:Up-Regulation
chemical_list: C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D004549:Elastin
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