title: Effects of added CeCl3 on resistance of fifth-instar larvae of silkworm to Bombyx mori nucleopolyhedrovirus infection.
abstract: One of the most important agents causing lethal disease in the silkworm is the Bombyx mori nucleopolyhedrovirus (BmNPV), while low-dose rare earths are demonstrated to increase immune capacity in animals. However, very little is known about the effects of added CeCl(3) on decreasing BmNPV infection of silkworm. The present study investigated the effects of added CeCl(3) to an artificial diet on resistance of fifth-instar larvae of silkworm to BmNPV infection. Our findings indicated that added CeCl(3) significantly decreased inhibition of growth and mortality of fifth-instar larvae caused by BmNPV infection. Furthermore, the added CeCl(3) obviously decreased lipid peroxidation level and accumulation of reactive oxygen species such as O(2)(-), H(2)O(2), (·)OH, and NO and increased activities of the antioxidant enzymes including superoxide dismutase, catalase, ascorbate peroxidase, glutathione peroxidase, ascorbate, and glutathione contents in the BmNPV-infected fifth-instar larvae. In addition, the added CeCl(3) could significantly promote acetylcholine esterase activity and attenuate the activity of inducible nitric oxide synthase in the BmNPV-infected fifth-instar larvae. These findings suggested that added CeCl(3) may relieve oxidative damage and neurotoxicity of silkworm caused by BmNPV infection via increasing antioxidant capacity and acetylcholine esterase activity.
authors.affiliation: Medical College of Soochow University, Suzhou 215123, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Rengping
authors.initials: R
authors.lastname: Hu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Cui
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Gong
authors.forename: Weide
authors.initials: W
authors.lastname: Shen
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D012831:Bombyx / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000821:virology*; D002563:Cerium / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000821:virology; D015227:Lipid Peroxidation / Q000187:drug effects; D017924:Nucleopolyhedroviruses / Q000187:drug effects* / Q000276:immunology; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002563:Cerium; D000110:Acetylcholinesterase; C026690:cerous chloride