title: Stabilized ribosome display for in vitro selection.
abstract: Ribosome display is a very effective and powerful technology for screening functional peptides or polypeptides in vitro. In ribosome display, each peptide or polypeptide (phenotype) links with its corresponding mRNA (genotype) through a ribosome. This link can be achieved by the absence of a stop codon in the mRNA, therefore stalling the ribosome at the end of translation with the nascent random sequence peptide extended by a spacer outside of the ribosome tunnel. In this chapter, we describe a method for the use of a further stabilized peptide-ribosome-mRNA complex for ribosome display.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan.
authors.forename: Shuta
authors.initials: S
authors.lastname: Hara
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Muye
authors.initials: M
authors.lastname: Xu
authors.forename: Zha
authors.initials: Z
authors.lastname: Li
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D019020:Directed Molecular Evolution; D007700:Kinetics; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D010455:Peptides; D012333:RNA, Messenger