title: Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping.
abstract: Concentrated cassava bagasse hydrolysate (CBH) containing 584.4 g/L glucose was studied for acetone-butanol-ethanol (ABE) fermentation with a hyper-butanol-producing Clostridium acetobutylicum strain in a fibrous bed bioreactor with gas stripping for continuous butanol recovery. With periodical nutrient supplementation, stable production of n-butanol from glucose in the CBH was maintained in the fed-batch fermentation over 263 h with an average sugar consumption rate of 1.28 g/L h and butanol productivity of 0.32±0.03 g/L h. A total of 108.5 g/L ABE (butanol: 76.4 g/L, acetone: 27.0 g/L, ethanol: 5.1 g/L) was produced, with an overall yield of 0.32±0.03 g/g glucose for ABE and 0.23±0.01 g/g glucose for butanol. The gas stripping process generated a product containing 10-16% (w/v) of butanol, ~4% (w/v) of acetone, a small amount of ethanol (<0.8%) and almost no acids, resulting in a highly concentrated butanol solution of ~64% (w/v) after phase separation.
authors.affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W. 19th Ave., Columbus, OH 43210, USA.
authors.forename: Congcong
authors.initials: C
authors.lastname: Lu
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
mesh_terms: D020001:1-Butanol / Q000302:isolation & purification* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D002482:Cellulose / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D002365:Manihot / Q000382:microbiology*; D010936:Plant Extracts / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D020001:1-Butanol; D002482:Cellulose; C027433:bagasse