title: Diagnosis of pneumococcal empyema using immunochromatographic test on pleural fluid and serotype distribution in Korean children.
abstract: To evaluate the diagnostic value of immunochromatographic test (ICT) on pleural fluid in diagnosing pneumococcal empyema in children and to determine pneumococcal serotypes, 62 exudative parapneumonic effusions from Korean children were tested with culture, ICT for S. pneumoniae, pneumococcal autolysin polymerase chain reaction (PCR), and subsequent sequencing. Of the 62 patients, culture was positive in 3 patients only (4.8%). Pneumococci were identified in 13 samples (21.0%) by sequencing-confirmed PCR and ICT, respectively. When pneumococcal empyema was defined by either positive culture or sequence confirmation, the sensitivity of ICT was 76.9% (10/13) and the specificity of ICT was 93.9%. Eight of 10 patients with positive ICT and culture-negative results had a history of prior antibiotics use, whereas none of the culture-proven cases had. Serotypes of PCR-positive samples were determined by multiplex PCR assays. Multiplex PCR detected serotypes 19A (6), 1 (1), 14 (1), 34 (1), and untypable (4). ICT on pleural fluid is a relatively sensitive and highly specific method for diagnosis of pneumococcal empyema, especially in children given prior antibiotics.
authors.affiliation: Department of Pediatrics, Seoul National University College of Medicine, Jongno-gu, Seoul 110-799, Korea.
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: So Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Jina
authors.initials: J
authors.lastname: Lee
authors.forename: Eun Hwa
authors.initials: EH
authors.lastname: Choi
authors.forename: Hoan Jong
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D016724:Empyema, Pleural / Q000175:diagnosis* / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D010996:Pleural Effusion / Q000382:microbiology*; D011018:Pneumonia, Pneumococcal / Q000150:complications* / Q000382:microbiology; D056910:Republic of Korea / Q000453:epidemiology; D012680:Sensitivity and Specificity; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000302:isolation & purification