title: Imaging workflow and calibration for CT-guided time-domain fluorescence tomography.
abstract: In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Tichauer
authors.forename: Robert W
authors.initials: RW
authors.lastname: Holt
authors.forename: Fadi
authors.initials: F
authors.lastname: El-Ghussein
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhu
authors.forename: Hamid
authors.initials: H
authors.lastname: Dehghani
authors.forename: Frederic
authors.initials: F
authors.lastname: Leblond
authors.forename: Brian W
authors.initials: BW
authors.lastname: Pogue
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