title: Antibacterial effects of six endodontic sealers.
abstract: OBJECTIVE
  The objective of this study was to perform an in vitro evaluation of the antibacterial properties of 6 endodontic sealers (Endomethasone C, Argoseal, Bioseal Normal, Acroseal, AH Plus, Sicura Seal).
  
  
  METHODS
  The agar diffusion test (well and paper disc methods) with Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans was used. For the well method, Petri dishes were inoculated with bacterial suspensions. Each well was completely filled with freshly mixed endodontic sealer. For the paper disc method, sterile paper discs were immersed in freshly mixed sealers and put on agar plates. Diameters of halos formed around the sealers were measured after 24 h and 48 h.
  
  
  METHODS
  The effects of well method and of paper disc method were analyzed by 1-way ANOVA.
  
  
  RESULTS
  Endomethasone C, Argoseal and Bioseal showed the largest inhibition halos for all the tested microorganisms, while Sicura Seal and AH Plus showed low antibacterial effects. Moreover, the comparison of well method and paper disc methods showed significant statistical differences (P<0,01) for all sealers and indicated a dose-dependent antimicrobial effect.
authors.affiliation: Department of Endodontics, University of Pavia, Pavia, Italy. c.poggio@unipv.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Poggio
authors.forename: Marco
authors.initials: M
authors.lastname: Lombardini
authors.forename: Marco
authors.initials: M
authors.lastname: Colombo
authors.forename: Alberto
authors.initials: A
authors.lastname: Dagna
authors.forename: Enrica
authors.initials: E
authors.lastname: Saino
authors.forename: Carla Renata
authors.initials: CR
authors.lastname: Arciola
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
mesh_terms: D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D004853:Epoxy Resins / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008768:Methylmethacrylates / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D013943:Thymol / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: C477053:Acroseal; D000900:Anti-Bacterial Agents; C097428:Bioseal; D004338:Drug Combinations; D004853:Epoxy Resins; D008768:Methylmethacrylates; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C011244:corticosteroid methanetriol mixture; C534916:epoxy resin-based root canal sealer; D005557:Formaldehyde; D013943:Thymol; C031667:IRM cement; D003907:Dexamethasone; D006854:Hydrocortisone