title: Bioremediation of the heavy metal complex dye Isolan Dark Blue 2SGL-01 by white rot fungus Irpex lacteus.
abstract: The present study was conducted to evaluate the decolorization and degradation of the chromium metal complex dye Isolan Dark Blue 2SGL-01 by Irpex lacteus, a white rot lignolytic fungus. I. lacteus effectively decolorized the sulphonated reactive dye at a high concentration of 250 mg/l over a wide range of pH values of 5-9 and temperatures between 20 and 35°C. Complete (100%) decolorization occurred within 96h, and I. lacteus demonstrated resistance to the metallic dye. UV-vis spectroscopy, HPLC, GC-MS, and FT-IR analyses of the extracted metabolites confirmed that the decolorization process occurred due to degradation of the dye and not merely by adsorption. GC-MS analysis indicated the formation of 1(2H)-naphthalenone, 3,4-dihydro- and 2-naphthalenol as the main metabolite. ICP analysis demonstrated the removal of 13.49% chromium, and phytotoxicity studies using germinated seeds of Vigna radiata and Brassica juncea demonstrated the nontoxic nature of the metabolites formed during the degradation of Isolan Dark Blue 2SGL-01 dye.
authors.affiliation: Department of Forest Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju, South Korea.
authors.forename: Duraisamy
authors.initials: D
authors.lastname: Kalpana
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Shim
authors.forename: Byung-Taek
authors.initials: BT
authors.lastname: Oh
authors.forename: Kalaiselvi
authors.initials: K
authors.lastname: Senthil
authors.forename: Yang Soo
authors.initials: YS
authors.lastname: Lee
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019216:Metals, Heavy / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004396:Coloring Agents; D019216:Metals, Heavy