title: Proteomic characterization of the greening process in rice seedlings using the MS spectral intensity-based label free method.
abstract: Illumination-induced greening in dark-grown plants is one of the most dramatic developmental processes known in plants. In our current study, we characterized the greening process of rice seedlings using comparative proteome analysis. We identified 886 different proteins in both whole cell lysates of illuminated and nonilluminated rice shoots and performed comparative proteome analysis based on the MS spectral intensities obtained for unique peptides from respective proteins. Furthermore, the changes in the levels of individual proteins were then compared with those of the corresponding mRNAs. The results revealed well-coordinated increases in the enzymes involved in the Calvin cycle at both the protein and mRNA levels during greening, and that the changes at the mRNA level precede those at the protein level. Although a much lower effect of illumination was found on the enzymes associated with glycolysis and the TCA cycle, coordinated increases during greening were evident for the enzymes involved in photorespiration and nitrogen assimilation as well as the components of the chloroplastic translational machinery. These results thus define the differential regulation of distinct biological systems during greening in rice and demonstrate the usefulness of comprehensive and comparative proteome analysis for the characterization of biological processes in plant cells.
authors.affiliation: Department of Applied Biological Chemistzry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Aki
authors.forename: Mikao
authors.initials: M
authors.lastname: Shigyo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sato
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishida
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yano
authors.forename: Tadakatsu
authors.initials: T
authors.lastname: Yoneyama
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yanagisawa
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism; D002952:Citric Acid Cycle / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006019:Glycolysis / Q000235:genetics; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D010446:Peptide Fragments / Q000737:chemistry; D010788:Photosynthesis / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D053719:Tandem Mass Spectrometry
chemical_list: D010446:Peptide Fragments; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D020543:Proteome; D002734:Chlorophyll