title: [Expression of TMPRSS3 in the rat cochlea  following kanamycin ototoxicity].
abstract: OBJECTIVE
  To establish the kanamycin-induced deafness model in SD rats, and to investigate the expression and significance of transmembrane protease, serine 3 (TMPRSS3) in the cochlea following kanamycin ototoxicity.
  
  
  METHODS
  A total of 40 male SD rats were randomly divided into 4 groups. The experimental rats received intramuscular kanamycin sulfate for 3, 7, and 14 consecutive days, and the control group were treated with normal saline for 14 days. Auditory brainstem responses (ABR) were obtained before and after the kanamycin administration. The expression of TMPRSS3 in the cochlea was identified and detected by immunohistochemistry and Western blot.
  
  
  RESULTS
  Kanamycin-induced deafness model in the SD rats was successfully established. ABR thresholds were increased and the expression of TMPRSS3 in the cochlea was reduced after the kanamycin injection (P<0.01).
  
  
  CONCLUSIONS
  TMPRSS3 may play an important role in normal cochlea function and involve in the process of aminoglycoside antibiotics induced deafness.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, Central South University, Changsha, China.
authors.forename: Anquan
authors.initials: A
authors.lastname: Peng
authors.forename: Shenglei
authors.initials: S
authors.lastname: Ge
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Dinghua
authors.initials: D
authors.lastname: Xie
authors.forename: Weijing
authors.initials: W
authors.lastname: Wu
authors.forename: Zi'an
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism*; D003638:Deafness / Q000139:chemically induced / Q000378:metabolism*; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D007612:Kanamycin / Q000633:toxicity*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012697:Serine Endopeptidases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D007612:Kanamycin; D012697:Serine Endopeptidases