title: Swept source optical coherence tomography as a tool for real time visualization and localization of electrodes used in electrophysiological studies of brain in vivo.
abstract: In studies of in vivo extracellular recording, we usually penetrate electrodes almost blindly into the neural tissue, in order to detect the neural activity from an expected target location at a certain depth. After the recording, it is necessary for us to determine the position of the electrodes precisely. Generally, to identify the position of the electrode, one method is to examine the postmortem tissue sample at micron resolution. The other method is using MRI and it does not have enough resolution to resolve the neural structures. To solve such problems, we propose swept source optical coherence tomography (SS-OCT) as a tool to visualize the cross-sectional image of the neural target structure along with the penetrating electrode. We focused on a rodent olfactory bulb (OB) as the target. We succeeded in imaging both the OB layer structure and the penetrating electrode, simultaneously. The method has the advantage of detecting the electrode shape and the position in real time, in vivo. These results indicate the possibility of using SS-OCT as a powerful tool for guiding the electrode into the target tissue precisely in real time and localizing the electrode tip during electrophysiological recordings.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Rajagopalan
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakamichi
authors.forename: Kei M
authors.initials: KM
authors.lastname: Igarashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kadono
authors.forename: Manabu
authors.initials: M
authors.lastname: Tanifuji
references.citation: Science. 1997 Oct 17;278(5337):463-7
references.pmid: 9334305
references.citation: Cell Tissue Res. 2004 Oct;318(1):275-88
references.pmid: 15322914
references.citation: J Neurophysiol. 2004 Jun;91(6):2532-40
references.pmid: 14960563
references.citation: Biomed Opt Express. 2011 Aug 1;2(8):2279-87
references.pmid: 21833364
references.citation: J Neurophysiol. 2009 Apr;101(4):1890-900
references.pmid: 19164106
references.citation: J Neurosci. 2009 Aug 19;29(33):10287-98
references.pmid: 19692603
references.citation: Opt Lett. 1997 Mar 1;22(5):340-2
references.pmid: 18183195
references.citation: Psychophysiology. 2003 Jul;40(4):529-41
references.pmid: 14570161
references.citation: J Neurosci Methods. 2009 Mar 30;178(1):162-73
references.pmid: 19121336
references.citation: Appl Opt. 2010 Jun 1;49(16):D30-61
references.pmid: 20517358
references.citation: Neuron. 2008 Sep 11;59(5):802-14
references.pmid: 18786363
references.citation: J Neurosci Methods. 2003 Mar 30;124(1):83-92
references.pmid: 12648767
references.citation: Opt Express. 2010 May 24;18(11):11772-84
references.pmid: 20589038
references.citation: Cereb Cortex. 2009 Aug;19(8):1870-88
references.pmid: 19068487
references.citation: Opt Express. 2003 Sep 8;11(18):2183-9
references.pmid: 19466106
references.citation: Brain. 2009 Apr;132(Pt 4):940-54
references.pmid: 19153151
references.citation: Chem Senses. 2008 Nov;33(9):779-92
references.pmid: 18635556
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169