title: Oct4 links multiple epigenetic pathways to the pluripotency network.
abstract: Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's critical regulatory activities. Here we employed an improved affinity purification approach combined with mass spectrometry to purify Oct4 protein complexes in mouse embryonic stem cells (mESCs), and discovered many novel Oct4 partners important for self-renewal and pluripotency of mESCs. Notably, we found that Oct4 is associated with multiple chromatin-modifying complexes with documented as well as newly proved functional significance in stem cell maintenance and somatic cell reprogramming. Our study establishes a solid biochemical basis for genetic and epigenetic regulation of stem cell pluripotency and provides a framework for exploring alternative factor-based reprogramming strategies.
authors.affiliation: Department of Developmental and Regenerative Biology, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Junjun
authors.initials: J
authors.lastname: Ding
authors.forename: Huilei
authors.initials: H
authors.lastname: Xu
authors.forename: Francesco
authors.initials: F
authors.lastname: Faiola
authors.forename: Avi
authors.initials: A
authors.lastname: Ma'ayan
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D065150:Cellular Reprogramming; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000166:cytology; D044127:Epigenesis, Genetic; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D050814:Octamer Transcription Factor-3 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D039904:Pluripotent Stem Cells / Q000737:chemistry / Q000166:cytology*; D025941:Protein Interaction Mapping / Q000379:methods*; D055748:SOXB1 Transcription Factors / Q000737:chemistry
chemical_list: D046912:Multiprotein Complexes; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse
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