title: Repair of rabbit osteochondral defects by an acellular technique with an ultrapurified alginate gel containing stromal cell-derived factor-1.
abstract: The objective of this study was to determine whether the local administration of stromal cell-derived factor-1 (SDF-1) using ultrapurified alginate gel (UPAL gel) could improve reparative tissues of osteochondral defects compared with those without treatment. For the investigation, a full-thickness osteochondral defect 4.5 mm in diameter and 3 mm in depth was created in the patella groove of the distal femur in rabbits. Local expression of SDF-1 protein was temporarily upregulated at 1 week after creating the osteochondral defect. The local administration of SDF-1 enhanced the migration of host cells, mainly bone marrow stromal cells (BMSCs), to the site of the osteochondral defect. In vitro cell migration assay supported this result. In the SDF-1 (UPAL gel containing SDF-1) treatment group, the histological scores and the compressive modulus of reparative tissues were significantly improved compared with the no-treatment and vehicle (UPAL gel without SDF-1) groups. On the other hand, SDF-1 did not influence the cellular proliferation and chondrogenesis of BMSCs. Based on the results obtained here, we speculate that SDF-1 enhances the reparative process of osteochondral injuries not through direct effects on the behavior of host cells, but through increased migration of host cells to the injured site. UPAL gel, as a vehicle material, may play an important role in chondrogenesis of recruited cells, mainly BMSCs. The cell-free approach with local administration of SDF-1 may be an effective strategy for developing a minimally invasive technique for cartilage tissue regeneration.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita-Ku, Sapporo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sukegawa
authors.forename: Norimasa
authors.initials: N
authors.lastname: Iwasaki
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kasahara
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Onodera
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Igarashi
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000737:chemistry / Q000627:therapeutic use*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D005269:Femur / Q000166:cytology / Q000293:injuries; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D054377:Chemokine CXCL12; D006603:Hexuronic Acids; D020723:Glucuronic Acid