title: Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis.
abstract: Babesiosis is one of the most important tick-borne diseases affecting livestock that can cause major economic losses worldwide particularly in the tropics. Control relies on controlling both the protozoan parasite and the tick vector. Antiprotozoal drugs are most commonly used for treatment, but problems on emergence of resistant strains and food residues are encountered. Longicin, a defensin-like peptide identified from the hard tick, Haemapysalis longicornis, as well as one of its synthetic partial analogs (P4), were previously reported to exert antimicrobial, fungicidal, and parasiticidal activity. Both longicin and P4 showed babesiacidal activity, in vitro and in vivo. Here, peptide fragments of P4 were studied for in vitro activity against bovine Babesia parasites. One of the peptide fragments, antimicrobial peptide 1 (AMP1), reduced the parasitemia of Babesia bigemina. No peptide had significant effect on Babesia bovis. The sequence of AMP1 corresponded to the longicin sequence which is associated with antiparasitic activity. Although AMP1 caused reduction in parasitemia of B. bigemina, the difference in morphology of the parasite compared with the control group was not statistically significant. However, the percentage occurrence of piroplasms decreased, whereas the abnormal pycnotic form increased. The results demonstrated that this shorter peptide retained the anti-babesial activity of the parent peptide, exerting an antiparasitic effect against a bovine Babesia species. Therefore, this short peptide can be considered for chemical synthesis as an alternative therapeutic agent for babesiosis.
authors.affiliation: Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan.
authors.forename: Remil Linggatong
authors.initials: RL
authors.lastname: Galay
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maeda
authors.forename: Kyaw Min
authors.initials: KM
authors.lastname: Aung
authors.forename: Rika
authors.initials: R
authors.lastname: Umemiya-Shirafuji
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001403:Babesia / Q000254:growth & development*; D001404:Babesiosis / Q000097:blood / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000188:drug therapy* / Q000469:parasitology*; D004912:Erythrocytes / Q000469:parasitology; D018512:Parasitemia / Q000188:drug therapy; D010446:Peptide Fragments / Q000494:pharmacology*
chemical_list: D010446:Peptide Fragments; C096944:longicin; D015107:4-Butyrolactone
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