title: Is degradable antibiotic coating for synthetic meshes provide protection against experimental animal infection after fascia repair?
abstract: The surgical repair of pelvic organ prolapse using synthetic mesh can fail because of slow or partial implant integration due to poor biocompatibility or infection. As systemic antibiotic prophylaxis has only limited success, we have developed a system that coats standard polypropylene mesh with clinically relevant antibiotics. Amoxicillin and ofloxacin are both released from the mesh in vitro at high levels over 3 days, preventing adhesion and biofilm formation by a clinical isolate of E. coli. In an in vivo incisional hernia repair model in rats, the antibiotic-coated mesh results in appropriate tissue integration with adequate vascularization and collagen formation. When implanted animals are infected with virulent E. coli, both antibiotic coatings provide full protection against infection (as assessed both clinically and microbiologically), thus demonstrating their bioavailability. This method is a specific approach for producing a therapeutic coating that could reduce postsurgical infections.
authors.affiliation: Department of Gynecology and Obstetrics, Caremeau University Hospital, Place Pr R Debré, 30000 Nimes, France; Max Mousseron Institute of Biomolecules, UMR CNRS 5247, Universities Montpellier 1 and Montpellier 2, Faculty of Pharmacy, 15 Avenue Charles Flahault, BP 14491, 34093 Montpellier, France. vincent.go@wanadoo.fr.
authors.forename: Vincent
authors.initials: V
authors.lastname: Letouzey
authors.forename: Jean Philippe
authors.initials: JP
authors.lastname: Lavigne
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
authors.forename: Jean
authors.initials: J
authors.lastname: Coudane
authors.forename: Renaud
authors.initials: R
authors.lastname: de Tayrac
authors.forename: David O
authors.initials: DO
authors.lastname: Callaghan
mesh_terms: D020341:Absorbable Implants; D000658:Amoxicillin / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D004343:Drug Implants; D004926:Escherichia coli / Q000254:growth & development; D046449:Hernia, Abdominal / Q000601:surgery*; D051381:Rats; D013526:Surgical Mesh
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D004343:Drug Implants; D000658:Amoxicillin