title: Two length variants of the microsatellite FH2295 as markers for body size of female Portuguese water dogs.
abstract: Genetic studies in purebred Portuguese water dogs (PWD) have previously identified genetic loci controlling skeleton size. The FH2295 genetic marker was reported to control 43.6% of the size variation in this breed. In the present study, we amplified and sequenced the genomic DNA from female PWD of different sizes in the region of the FH2295 genetic marker. Polymerase chain reaction (PCR) products of 700 and 800 bp were generated and sequencing revealed the presence of a microsatellite marker including either 5 or 24 repeats of the tetranucleotide sequence "CTTT". Dogs were divided into groups based on their genotypes: homozygote for the short allele (II) or homozygote for the long allele (BB) or heterozygote (IB). The smallest dogs were homozygous with 24 repeats and the largest dogs were homozygous with five repeats. Genetic transmission of the microsatellite marker appears to follow Mendelian laws since all puppies born to a homozygous small dog genotyped "BB" included one or two "B" allele. We applied a PCR method to characterize the sequence of the previously identified dog genetic marker FH2295 and propose that the length of the microsatellite identified could be used as a predictor for the body size of female PWD.
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada.
authors.forename: S C
authors.initials: SC
authors.lastname: Bérubé
authors.forename: P R
authors.initials: PR
authors.lastname: Johnsson
authors.forename: N
authors.initials: N
authors.lastname: Bunimov
authors.forename: C
authors.initials: C
authors.lastname: Boivin
authors.forename: O
authors.initials: O
authors.lastname: Laneuville
mesh_terms: D000483:Alleles; D000818:Animals; D049628:Body Size / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000235:genetics; D004285:Dogs / Q000235:genetics* / Q000502:physiology; D005260:Female; D005819:Genetic Markers; D016678:Genome; D060005:Genotyping Techniques; D006720:Homozygote; D040582:Inheritance Patterns; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers; D004247:DNA
references.citation: Genome Res. 2005 Dec;15(12):1820-4
references.pmid: 16339380
references.citation: Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9930-5
references.pmid: 12114542
references.citation: Genome Res. 2005 Dec;15(12):1825-30
references.pmid: 16339381
references.citation: Am J Med Genet A. 2004 Jan 30;124A(3):239-47
references.pmid: 14708095
references.citation: Science. 2007 Apr 6;316(5821):112-5
references.pmid: 17412960
references.citation: Endocrinology. 1999 Jul;140(7):3391-4
references.pmid: 10385438
references.citation: Int J Immunogenet. 2006 Jun;33(3):179-84
references.pmid: 16712648
references.citation: J Hered. 2010 Sep-Oct;101(5):612-7
references.pmid: 20562213
references.citation: Mamm Genome. 2008 Oct-Dec;19(10-12):713-23
references.pmid: 19020935
references.citation: J Hered. 1999 Jan-Feb;90(1):43-51
references.pmid: 9987902
references.citation: Endocrinology. 2007 Jul;148(7):3122-30
references.pmid: 17395707