title: Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis.
abstract: RNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence reads mapping outside of ribosomal RNA genes represent non-coding RNA, and the density of reads mapping to intergenic regions was more than two-fold higher than that mapping to annotated coding sequences. Selected sRNAs were found at increased abundance in stationary phase cultures and accumulated to remarkably high levels in the lungs of chronically infected mice, indicating a potential contribution to pathogenesis. The ability of tubercle bacilli to adapt to changing environments within the host is critical to their ability to cause disease and to persist during drug treatment; it is likely that novel post-transcriptional regulatory networks will play an important role in these adaptive responses.
authors.affiliation: Division of Mycobacterial Research, MRC National Institute for Medical Research, London, United Kingdom. karnvig@nimr.mrc.ac.uk
authors.forename: Kristine B
authors.initials: KB
authors.lastname: Arnvig
authors.forename: Iñaki
authors.initials: I
authors.lastname: Comas
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.forename: Joanna
authors.initials: J
authors.lastname: Houghton
authors.forename: Helena I
authors.initials: HI
authors.lastname: Boshoff
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Croucher
authors.forename: Graham
authors.initials: G
authors.lastname: Rose
authors.forename: Timothy T
authors.initials: TT
authors.lastname: Perkins
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Young
mesh_terms: D000818:Animals; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000472:pathogenicity; D020411:Oligonucleotide Array Sequence Analysis; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics*; D022661:RNA, Untranslated / Q000032:analysis / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D012329:RNA, Bacterial; D022661:RNA, Untranslated
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