title: Myriophyllum aquaticum versus Lemna minor: sensitivity and recovery potential after exposure to atrazine.
abstract: The relative sensitivity and recovery potential of two aquatic macrophyte species, Lemna minor and Myriophyllum aquaticum, exposed to atrazine (concentration ranges 80-1,280 µg/L and 40-640 µg/L, respectively) were evaluated using slightly adapted standard protocol for Lemna spp.: relative growth rates (RGR) and yield of both plants were measured in 3-d-long intervals during the exposure and recovery phase. Myriophyllum aquaticum was also exposed to atrazine-spiked sediment (0.1-3.7 µg/g) in a water-free system. The results of M. aquaticum sediment contact tests showed that root- and shoot-based growth parameters are equally sensitive endpoints. In the water (sediment-free) test system, L. minor recovered after short (3 d) and longer exposure (7 d) to all atrazine concentrations after only a 5- to 6-d-long recovery phase. The recovery of M. aquaticum after short exposure was slower and less efficient: after 12 d of recovery phase the final biomass of plants exposed to 380 and 640 µg/L was below the initial values. The last interval RGR provides a good indication of plant recovery potential regardless of species growth strategy. If compared to L. minor, the difference in growth rate, sensitivity, lag phase, recovery potential from water-column substances, and also suitability for studies investigating the effect of sediment-bound pollutants advocates the use of M. aquaticum as an additional macrophyte species in risk assessment.
authors.affiliation: Laboratory for Ecotoxicology, University of Novi Sad, Novi Sad, Serbia. teodorovic@beocity.net
authors.forename: Ivana
authors.initials: I
authors.lastname: Teodorović
authors.forename: Varja
authors.initials: V
authors.lastname: Knežević
authors.forename: Tanja
authors.initials: T
authors.lastname: Tunić
authors.forename: Mićo
authors.initials: M
authors.lastname: Cučak
authors.forename: Jelena Nikolić
authors.initials: JN
authors.lastname: Lečić
authors.forename: Anita
authors.initials: A
authors.lastname: Leovac
authors.forename: Ivana Ivančev
authors.initials: II
authors.lastname: Tumbas
mesh_terms: D029064:Araceae / Q000187:drug effects* / Q000254:growth & development; D001280:Atrazine / Q000633:toxicity*; D018533:Biomass; D006540:Herbicides / Q000633:toxicity*; D019684:Magnoliopsida / Q000187:drug effects* / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018570:Risk Assessment; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D001280:Atrazine