title: [Sequencing and analyses of the adenovirus polymerase gene in fecal samples of captively bred Rhesus macaques].
abstract: OBJECTIVE
  In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate (NHP) populations.
  
  
  METHODS
  We examined a colony of captively bred rhesus macaques (Macaca mulatta) in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses.
  
  
  RESULTS
  Among the 57 animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA. The results suggested that the viral DNA clones were primarily segregated into two large groups: SAdV-6 (2 non-redundant sequences) and SAdV-7 (9 non-redundant sequences). In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively.
  
  
  CONCLUSIONS
  Our data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the adenoviruses in the gastrointestinal tract of the study animals. heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.
authors.affiliation: Vaccine Research Institute, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jian-xi
authors.initials: JX
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Shi-yu
authors.initials: SY
authors.lastname: Qian
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Gang Li
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D005243:Feces / Q000821:virology*; D005260:Female; D008253:Macaca mulatta / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase