title: Osteogenesis of human mesenchymal stem cells on micro-patterned surfaces.
abstract: Osteogenic responses of human mesenchymal stromal cells (hMSCs) were compared on square-patterned, inverse square-patterned, and planar titanium, chromium, diamond-like carbon (DLC), and tantalum; hypothesis was that both the materials and patterns affect osteogenesis. Samples were produced using photolithography and physical vapor deposition. Early-marker alkaline phosphatase (ALP) and mid-markers, small body size and mothers against decapentaplegic-related protein-1 (SMAD1), runt-related transcription factor-2 (RUNX2), and osteopontin were studied using quantitative real-time polymerase chain reaction. ALP and hydroxyapatite, were colorimetrically studied. ALP reached highest values on both patterned titanium samples, but mid-markers disclosed that it was already lagging behind planar and inverse patterned tantalum. Hydroxyapatite formation disclosed that osteo-induced hMSCs passed all the differentiation stages (except on planar chromium). Presence of hydroxyapatite disclosed that both types of patterning promoted (p < 0.001) osteogenesis compared to planar samples. Results suggest that the osseocompatibility/integration of implants could be improved by changing the monotonous and featureless implant-host interface into micro-patterned interface to provide physical differentiation cues.
authors.affiliation: Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Haartmaninkatu 8, FI-00029 HUS, Finland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Kaivosoja
authors.forename: Sami
authors.initials: S
authors.lastname: Myllymaa
authors.forename: Yuya
authors.initials: Y
authors.lastname: Takakubo
authors.forename: Hannu
authors.initials: H
authors.lastname: Korhonen
authors.forename: Katja
authors.initials: K
authors.lastname: Myllymaa
authors.forename: Yrjö T
authors.initials: YT
authors.lastname: Konttinen
authors.forename: Reijo
authors.initials: R
authors.lastname: Lappalainen
authors.forename: Michiaki
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002857:Chromium / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D013499:Surface Properties; D013635:Tantalum / Q000737:chemistry* / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D002857:Chromium; D013635:Tantalum; D002244:Carbon; D014025:Titanium; D000469:Alkaline Phosphatase