title: Immortalized gingival fibroblasts as a cytotoxicity test model for dental materials.
abstract: In vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.
authors.affiliation: Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 Project, Seoul, Republic of Korea.
authors.forename: Rasika P
authors.initials: RP
authors.lastname: Illeperuma
authors.forename: Young J
authors.initials: YJ
authors.lastname: Park
authors.forename: Jin M
authors.initials: JM
authors.lastname: Kim
authors.forename: Jung Y
authors.initials: JY
authors.lastname: Bae
authors.forename: Zhong M
authors.initials: ZM
authors.lastname: Che
authors.forename: Hwa K
authors.initials: HK
authors.lastname: Son
authors.forename: Mi R
authors.initials: MR
authors.lastname: Han
authors.forename: Kwang M
authors.initials: KM
authors.lastname: Kim
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
mesh_terms: D002453:Cell Cycle; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D003764:Dental Materials; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology*; D006801:Humans
chemical_list: D003764:Dental Materials
references.citation: Dent Mater. 2008 Feb;24(2):281-7
references.pmid: 18023858
references.citation: Dent Mater. 2008 Nov;24(11):1461-7
references.pmid: 18439669
references.citation: Dent Mater. 2010 Jan;26(1):51-8
references.pmid: 19767088
references.citation: J Dent Res. 2009 Oct;88(10):916-21
references.pmid: 19783799
references.citation: Dent Mater. 2008 Sep;24(9):1201-6
references.pmid: 18336898
references.citation: Curr Biol. 1999 Sep 9;9(17):939-45
references.pmid: 10508581
references.citation: Dent Mater. 2009 Nov;25(11):1358-62
references.pmid: 19604573
references.citation: Dent Mater. 2009 Nov;25(11):1446-52
references.pmid: 19647309
references.citation: Dent Mater. 2009 Mar;25(3):360-8
references.pmid: 18849067
references.citation: Drug Chem Toxicol. 2008;31(3):339-52
references.pmid: 18622869
references.citation: Dent Mater. 2008 Jan;24(1):134-40
references.pmid: 17920114
references.citation: Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9363-7
references.pmid: 7568133
references.citation: Biomaterials. 2008 Jul;29(20):2941-53
references.pmid: 18440630
references.citation: J Oral Pathol Med. 2004 Aug;33(7):417-23
references.pmid: 15250834
references.citation: Invest Ophthalmol Vis Sci. 2005 Feb;46(2):470-8
references.pmid: 15671271
references.citation: EMBO J. 1992 May;11(5):1921-9
references.pmid: 1582420
references.citation: Exp Cell Res. 1961 Dec;25:585-621
references.pmid: 13905658
references.citation: Dent Mater. 2009 Dec;25(12):1556-63
references.pmid: 19716169
references.citation: Annu Rev Biochem. 1996;65:337-65
references.pmid: 8811183
references.citation: Biomed Mater. 2010 Aug;5(4):044102
references.pmid: 20683133
references.citation: Dent Mater. 2010 Oct;26(10):1017-23
references.pmid: 20650509
references.citation: Dent Mater. 2009 Nov;25(11):1371-5
references.pmid: 19615734
references.citation: J Endod. 2010 Jun;36(6):1056-60
references.pmid: 20478465
references.citation: Tissue Eng Part A. 2008 Dec;14(12):1989-97
references.pmid: 18673088
references.citation: Dent Mater. 2009 Dec;25(12):1551-5
references.pmid: 19682734
references.citation: Int Endod J. 2010 Mar;43(3):251-7
references.pmid: 20158537
references.citation: J Dent Res. 1995 Aug;74(8):1513-20
references.pmid: 7560408
references.citation: Dent Mater. 2008 Aug;24(8):1025-35
references.pmid: 18237774
references.citation: Nat Genet. 1999 Jan;21(1):115-8
references.pmid: 9916803
references.citation: J Oral Pathol Med. 1999 Feb;28(2):64-71
references.pmid: 9950252