title: Decellularization as an anticalcification method in stentless bovine pericardium valve prosthesis: a study in sheep.
abstract: OBJECTIVE
  The objective was to analyze the decellularization process with SDS in glutaraldehyde-preserved bovine pericardium as an anticalcification method in a circulatory sheep model.
  
  
  METHODS
  The valved tubs were implanted in pulmonary artery position in sheep by 180 days. The animals were divided in two groups of 8 animals: control group--glutaraldehyde-preserved bovine pericardium and the study group--decellularized bovine pericardium with 0,1% SDS and glutaraldehyde-preserved. After explantation the tubs were analized by x-ray macroscopy, hematoxilin-eosin, alizarin-red and Russel-Movatz pentacromic histology. The calcium content was measured by flame atomic absorption spectrometry.
  
  
  RESULTS
  There was no early mortality, but two animals in each group died during the study. All cusps in the control group were severely calcified and in some points in the conduits, while the decellularized group did not show macroscopic calcification. Data were proved by x-ray and histologycal exams. The matrix was preserved in histologycal analysis in decellularized group, without gross calcification. The wall conduits calcium content was 35,25 ± 42,13 µg/mg in the control group versus 15,75 ± 10,44 µg/mg in the decellularized one: in the cusps was 264,4 ± 126,16 µg/mg in control group versus 94,29 ± 27,05 µg/mg in decellularized group (P = 0,009).
  
  
  CONCLUSIONS
  The decellularization with 0.1% SDS was effective as an anticalcification method in bovine pericardial grafts implanted in a sheep circulatory model for 180 days.
authors.affiliation: Santa Casa de Curitiba, Curitiba, PR, Brasil. claudineicl@yahoo.com.br
authors.forename: Claudinei
authors.initials: C
authors.lastname: Collatusso
authors.forename: João Gabriel
authors.initials: JG
authors.lastname: Roderjan
authors.forename: Eduardo Discher
authors.initials: ED
authors.lastname: Vieira
authors.forename: Nelson Itiro
authors.initials: NI
authors.lastname: Myague
authors.forename: Lúcia de
authors.initials: Ld
authors.lastname: Noronha
authors.forename: Francisco Diniz Affonso da
authors.initials: FD
authors.lastname: Costa
mesh_terms: D000818:Animals; D001705:Bioprosthesis / Q000009:adverse effects*; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000032:analysis; D006350:Heart Valve Prosthesis / Q000009:adverse effects*; D023421:Models, Animal; D011664:Pulmonary Valve / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D011859:Radiography; D011897:Random Allocation; D012756:Sheep; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D018709:Statistics, Nonparametric; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D012967:Sodium Dodecyl Sulfate; D002118:Calcium