title: Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate.
abstract: An N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues was crystallized both in its apo form and in a binary complex with glucose 6-phosphate. The crystals both belonged to space group P2(1) and diffracted to 2.85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure.
authors.affiliation: Redox Biology of Trypanosomes Laboratory, Institut Pasteur de Montevideo, Mataojo 2020, 11400 Montevideo, Uruguay.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ortíz
authors.forename: Nicole
authors.initials: N
authors.lastname: Larrieux
authors.forename: Andrea
authors.initials: A
authors.lastname: Medeiros
authors.forename: Horacio
authors.initials: H
authors.lastname: Botti
authors.forename: Marcelo
authors.initials: M
authors.lastname: Comini
authors.forename: Alejandro
authors.initials: A
authors.lastname: Buschiazzo
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D005954:Glucosephosphate Dehydrogenase
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