title: Decolorization of textile dyes by Alishewanella sp. KMK6.
abstract: Alishewanella sp. strain KMK6 was isolated from textile dye-contaminated soil. The strain was able to decolorize and degrade different azo dyes and displayed high dye degradation ability and tolerance. The bacterium could completely degrade 2.5 g l(-1) dye, Reactive Blue 59 within 6 h. The induction in the level of cytochrome P-450 and activities of azoreductase and NADH-dichlorophenolindophenol reductase were observed in the cells after dye decolorization indicating the role of these enzymes. The intermediates of Reactive Blue 59 degradation were identified by high-performance liquid chromatography, gas chromatography and mass spectroscopy, and Fourier transform infrared spectroscopy. The ecotoxicity has been evaluated for dye and its metabolites by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (a yellow tetrazole) and comet assay, and it revealed that the dye degradation products were nontoxic.
authors.affiliation: Biochemistry Division, Department of Chemistry, University of Pune, Pune, India.
authors.forename: Yogesh M
authors.initials: YM
authors.lastname: Kolekar
authors.forename: Kisan M
authors.initials: KM
authors.lastname: Kodam
mesh_terms: D034241:Alteromonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D004785:Environmental Pollutants / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D011808:Quinone Reductases / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D012336:RNA, Ribosomal, 16S; D009243:NAD; D003577:Cytochrome P-450 Enzyme System; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; C033633:dichlorophenolindophenol reductase; D009601:Nitroreductases; C021106:azoreductase