title: Low-density lipoprotein receptor-related protein 2 gene is associated with egg-quality traits in dwarf layers.
abstract: Some members of the low-density lipoprotein receptor (LDLR) family play important roles in the regulation of lipoprotein metabolism and egg quality traits. Low-density lipoprotein receptor-related protein 2 (LRP2) gene belongs to the LDLR super family, and widely expresses in many tissues. This work identified and genotyped 1 single-nucleotide polymorphism (SNP), T14347C, at 3'-UTR of the LRP2 using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS), and analyzed the effects of the SNP (T14347C) on egg-quality traits in 544 dwarf hens from 44 sire families. Frequencies of this SNP in the studied population did not agree with the Hardy-Weinberg equilibrium (P < 0.0001). Egg weight, albumen weight, albumen height, and albumen ratio of the TT genotype were significantly higher than those of the CC genotype (P < 0.05), whereas eggshell ratio of the TT genotype was significantly lower than that of the CC genotype (P < 0.05). The relative expression level of the LRP2 gene in the magnum was determined by real-time quantitative PCR. The gene expression of genotype CC individuals was significantly higher than that of TT and CT birds (P < 0.05). By combining both genetic effects and expression analyses results, we propose that the LRP2 gene is a good candidate gene, exhibiting a key role in albumen formation processes.
authors.affiliation: China Agricultural University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Z C
authors.initials: ZC
authors.lastname: Hou
authors.forename: Z X
authors.initials: ZX
authors.lastname: Chen
authors.forename: J X
authors.initials: JX
authors.lastname: Zheng
authors.forename: S R
authors.initials: SR
authors.lastname: Chen
authors.forename: L J
authors.initials: LJ
authors.lastname: Qu
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: G Y
authors.initials: GY
authors.lastname: Xu
authors.forename: N
authors.initials: N
authors.lastname: Yang
mesh_terms: D000483:Alleles; D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000502:physiology*; D004531:Eggs / Q000592:standards*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D026561:Low Density Lipoprotein Receptor-Related Protein-2 / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D026561:Low Density Lipoprotein Receptor-Related Protein-2