title: Crystallization and preliminary X-ray crystallographic analysis of ligand-free and arginine-bound forms of Thermotoga maritima arginine-binding protein.
abstract: The arginine-binding protein from Thermotoga maritima (TmArgBP) is an arginine-binding component of the ATP-binding cassette (ABC) transport system in this hyperthermophilic bacterium. This protein is endowed with an extraordinary stability towards thermal and chemical denaturation. Its structural characterization may provide useful insights for the clarification of structure-stability relationships and for the design of new biosensors. Crystallization trials were set up for both arginine-bound and ligand-free forms of TmArgBP and crystals suitable for crystallographic investigations were obtained for both forms. Ordered crystals of the arginine adduct of TmArgBP could only be obtained by using the detergent LDAO as an additive to the crystallization medium. These crystals were hexagonal, with unit-cell parameters a = 78.2, c = 434.7 Å, and diffracted to 2.7 Å resolution. The crystals of the ligand-free form were orthorhombic, with unit-cell parameters a = 51.8, b = 91.9, c = 117.9 Å, and diffracted to 2.25 Å resolution.
authors.affiliation: Institute of Biostructures and Bioimaging, CNR, Via Mezzocannone 16, I-80134 Naples, Italy. alessia.ruggiero@unina.it
authors.forename: Alessia
authors.initials: A
authors.lastname: Ruggiero
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Dattelbaum
authors.forename: Anna
authors.initials: A
authors.lastname: Pennacchio
authors.forename: Luisa
authors.initials: L
authors.lastname: Iozzino
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Luchansky
authors.forename: Bryan S
authors.initials: BS
authors.lastname: Der
authors.forename: Rita
authors.initials: R
authors.lastname: Berisio
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
authors.forename: Luigi
authors.initials: L
authors.lastname: Vitagliano
mesh_terms: D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D020124:Thermotoga maritima / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D018398:Homeodomain Proteins; D008024:Ligands; D001120:Arginine
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