title: [Study on macrophages infected with Mycobacterium tuberculosis H37Ra in vitro].
abstract: OBJECTIVE
  To study the production of nitric oxide and secretion of cytokines after infection of macrophages with Mycobacterium tuberculosis H37Ra.
  
  
  METHODS
  24 hours after infection of RAW264.7 cells with Mycobacterium tuberculosis H37Ra,  the production of NO and H(2);O(2); as well as the secretion levels of IL-12 and TNF-α in the supernatants of culture were determined by Griess method,  chemical method and ELISA assay respectively. The expression of IL-12 and TNF-α mRNA in macrophages was detected by reverse transcription polymerase chain reaction (RT- PCR).
  
  
  RESULTS
  Macrophages infected with Mycobacterium tuberculosis H37Ra produced effectively more NO,  H(2);O(2);,  and enhanced the release of IL-12,  TNF-α and the expression of IL-12,  TNF-α mRNA (P<0.05).
  
  
  CONCLUSIONS
  Mycobacterium tuberculosis H37Ra can induce the production of more nitric oxide and cytokines which play important roles in the host immune response.
authors.affiliation: Department of Medical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing, China. laichengliu@126.com
authors.forename: Lai-cheng
authors.initials: LC
authors.lastname: Liu
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Wang
authors.forename: Xian-yu
authors.initials: XY
authors.lastname: Lu
authors.forename: You-qun
authors.initials: YQ
authors.lastname: Zeng
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.forename: Peng-hui
authors.initials: PH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006861:Hydrogen Peroxide / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D009569:Nitric Oxide; D006861:Hydrogen Peroxide