title: Directly created electrostatic micro-domains on hydroxyapatite: probing with a Kelvin Force probe and a protein.
abstract: Micro-domains of modified surface potential (SP) were created on hydroxyapatite films by direct patterning by mid-energy focused electron beam, typically available as a microprobe of Scanning Electron Microscopes. The SP distribution of these patterns has been studied on sub-micrometer scale by the Kelvin Probe Force Microscopy method as well as lysozyme adsorption. Since the lysozyme is positively charged at physiological pH, it allows us to track positively and negatively charged areas of the SP patterns. Distribution of the adsorbed proteins over the domains was in good agreement with the observed SP patterns.
authors.affiliation: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia. tomas.plecenik@fmph.uniba.sk
authors.forename: Tomas
authors.initials: T
authors.lastname: Plecenik
authors.forename: Sylvain
authors.initials: S
authors.lastname: Robin
authors.forename: Maros
authors.initials: M
authors.lastname: Gregor
authors.forename: Martin
authors.initials: M
authors.lastname: Truchly
authors.forename: Sidney
authors.initials: S
authors.lastname: Lang
authors.forename: Abbasi
authors.initials: A
authors.lastname: Gandhi
authors.forename: Miroslav
authors.initials: M
authors.lastname: Zahoran
authors.forename: Fathima
authors.initials: F
authors.lastname: Laffir
authors.forename: Tewfik
authors.initials: T
authors.lastname: Soulimane
authors.forename: Melinda
authors.initials: M
authors.lastname: Vargova
authors.forename: Gustav
authors.initials: G
authors.lastname: Plesch
authors.forename: Peter
authors.initials: P
authors.lastname: Kus
authors.forename: Andrej
authors.initials: A
authors.lastname: Plecenik
authors.forename: S A M
authors.initials: SA
authors.lastname: Tofail
mesh_terms: D017886:Durapatite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes; D011506:Proteins / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D015335:Molecular Probes; D011506:Proteins; D017886:Durapatite
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