title: Transcriptomic coordination in the human metabolic network reveals links between n-3 fat intake, adipose tissue gene expression and metabolic health.
abstract: Understanding the molecular link between diet and health is a key goal in nutritional systems biology. As an alternative to pathway analysis, we have developed a joint multivariate and network-based approach to analysis of a dataset of habitual dietary records, adipose tissue transcriptomics and comprehensive plasma marker profiles from human volunteers with the Metabolic Syndrome. With this approach we identified prominent co-expressed sub-networks in the global metabolic network, which showed correlated expression with habitual n-3 PUFA intake and urinary levels of the oxidative stress marker 8-iso-PGF(2α). These sub-networks illustrated inherent cross-talk between distinct metabolic pathways, such as between triglyceride metabolism and production of lipid signalling molecules. In a parallel promoter analysis, we identified several adipogenic transcription factors as potential transcriptional regulators associated with habitual n-3 PUFA intake. Our results illustrate advantages of network-based analysis, and generate novel hypotheses on the transcriptomic link between habitual n-3 PUFA intake, adipose tissue function and oxidative stress.
authors.affiliation: Nutrigenomics Research Group, UCD Conway Institute, University College Dublin, Ireland.
authors.forename: Melissa J
authors.initials: MJ
authors.lastname: Morine
authors.forename: Audrey C
authors.initials: AC
authors.lastname: Tierney
authors.forename: Ben
authors.initials: B
authors.lastname: van Ommen
authors.forename: Hannelore
authors.initials: H
authors.lastname: Daniel
authors.forename: Sinead
authors.initials: S
authors.lastname: Toomey
authors.forename: Ingrid M F
authors.initials: IM
authors.lastname: Gjelstad
authors.forename: Isobel C
authors.initials: IC
authors.lastname: Gormley
authors.forename: Pablo
authors.initials: P
authors.lastname: Pérez-Martinez
authors.forename: Christian A
authors.initials: CA
authors.lastname: Drevon
authors.forename: Jose
authors.initials: J
authors.lastname: López-Miranda
authors.forename: Helen M
authors.initials: HM
authors.lastname: Roche
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D019295:Computational Biology; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006304:Health Status; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008954:Models, Biological; D015999:Multivariate Analysis; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic
chemical_list: D015525:Fatty Acids, Omega-3
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