title: An improved rapid method for the preparation of D-rhamnose.
abstract: A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was crystallized. The sugar activity was demonstrated immunochemically. The advantages of the method are that it expedites and simplifies the extraction of D-rhamnose and increases its yield.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia. room308@ibppm.sgu.ru
authors.forename: Olga N
authors.initials: ON
authors.lastname: Smol'kina
authors.forename: Natalya S
authors.initials: NS
authors.lastname: Shishonkova
authors.forename: Yulia P
authors.initials: YP
authors.lastname: Fedonenko
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Konnova
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Ignatov
mesh_terms: D015806:Azospirillum brasilense / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel; D019081:O Antigens / Q000737:chemistry; D012210:Rhamnose / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D019081:O Antigens; D012210:Rhamnose