title: GLUT 5 is not over-expressed in breast cancer cells and patient breast cancer tissues.
abstract: F18 2-Fluoro 2-deoxyglucose (FDG) has been the gold standard in positron emission tomography (PET) oncologic imaging since its introduction into the clinics several years ago. Seeking to complement FDG in the diagnosis of breast cancer using radio labeled fructose based analogs, we investigated the expression of the chief fructose transporter-GLUT 5 in breast cancer cells and human tissues. Our results indicate that GLUT 5 is not over-expressed in breast cancer tissues as assessed by an extensive immunohistochemistry study. RT-PCR studies showed that the GLUT 5 mRNA was present at minimal amounts in breast cancer cell lines. Further knocking down the expression of GLUT 5 in breast cancer cells using RNA interference did not affect the fructose uptake in these cell lines. Taken together these results are consistent with GLUT 5 not being essential for fructose uptake in breast cancer cells and tissues.
authors.affiliation: Molecular Imaging Program, Department of Radiology, Division of Nuclear Medicine, Stanford, California, United States of America.
authors.forename: Gayatri
authors.initials: G
authors.lastname: Gowrishankar
authors.forename: Sabine
authors.initials: S
authors.lastname: Zitzmann-Kolbe
authors.forename: Anitha
authors.initials: A
authors.lastname: Junutula
authors.forename: Robert
authors.initials: R
authors.lastname: Reeves
authors.forename: Jelena
authors.initials: J
authors.lastname: Levi
authors.forename: Ananth
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Kjerstin
authors.initials: K
authors.lastname: Bruus-Jensen
authors.forename: John
authors.initials: J
authors.lastname: Cyr
authors.forename: Ludger
authors.initials: L
authors.lastname: Dinkelborg
authors.forename: Sanjiv S
authors.initials: SS
authors.lastname: Gambhir
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003571:Cytochalasin B / Q000494:pharmacology; D017931:DNA Primers; D005260:Female; D005632:Fructose / Q000378:metabolism; D055785:Gene Knockdown Techniques; D051296:Glucose Transporter Type 5 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D034741:RNA, Small Interfering
chemical_list: D017931:DNA Primers; D051296:Glucose Transporter Type 5; D034741:RNA, Small Interfering; C495726:SLC2A5 protein, human; D005632:Fructose; D003571:Cytochalasin B
references.citation: J Am Chem Soc. 2009 Dec 9;131(48):17591-6
references.pmid: 19860409
references.citation: Mol Membr Biol. 2001 Oct-Dec;18(4):247-56
references.pmid: 11780753
references.citation: J Clin Endocrinol Metab. 1999 Sep;84(9):3228-34
references.pmid: 10487692
references.citation: Exp Physiol. 1998 Mar;83(2):267-73
references.pmid: 9568488
references.citation: J Nucl Med. 2003 Sep;44(9):1426-31
references.pmid: 12960187
references.citation: J Cell Physiol. 2006 Jun;207(3):614-27
references.pmid: 16523487
references.citation: CA Cancer J Clin. 2005 Jan-Feb;55(1):10-30
references.pmid: 15661684
references.citation: J Nucl Med. 2003 Feb;44(2):224-39
references.pmid: 12571214
references.citation: Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1847-52
references.pmid: 8700847
references.citation: Cancer. 1993 Nov 15;72(10):2979-85
references.pmid: 8221565
references.citation: Blood. 1997 Jun 1;89(11):4190-5
references.pmid: 9166863
references.citation: Nature. 2009 Feb 12;457(7231):910-4
references.pmid: 19212411
references.citation: Biophys J. 1996 Jan;70(1):14-21
references.pmid: 8770183
references.citation: J Pathol. 2008 Dec;216(4):387-93
references.pmid: 18853439
references.citation: J Cell Biochem. 2004 Dec 15;93(6):1134-42
references.pmid: 15449313
references.citation: Biochem J. 1993 Oct 15;295 ( Pt 2):329-41
references.pmid: 8240230
references.citation: Pathol Oncol Res. 1998;4(2):115-20
references.pmid: 9654596
references.citation: Semin Nucl Med. 2004 Jan;34(1):56-69
references.pmid: 14735459
references.citation: Clin Cancer Res. 2005 Apr 15;11(8):2785-808
references.pmid: 15837727
references.citation: J Clin Oncol. 2000 Oct 15;18(20):3495-502
references.pmid: 11032590
references.citation: J Cell Biol. 1994 Sep;126(5):1123-6
references.pmid: 8063851
references.citation: Bioconjug Chem. 2007 May-Jun;18(3):628-34
references.pmid: 17444608
references.citation: Bioorg Med Chem. 2009 Aug 1;17(15):5488-95
references.pmid: 19586773
references.citation: J Biol Chem. 1992 Jul 25;267(21):14523-6
references.pmid: 1634504