title: Transcriptional regulation of Arabidopsis LEAFY COTYLEDON2 involves RLE, a cis-element that regulates trimethylation of histone H3 at lysine-27.
abstract: LEAFY COTYLEDON2 (LEC2) is a master regulator of seed development in Arabidopsis thaliana. In vegetative organs, LEC2 expression is negatively regulated by Polycomb Repressive Complex2 (PRC2) that catalyzes histone H3 Lys 27 trimethylation (H3K27me3) and plays a crucial role in developmental phase transitions. To characterize the cis-regulatory elements involved in the transcriptional regulation of LEC2, molecular dissections and functional analyses of the promoter region were performed in vitro, both in yeast and in planta. Two cis-activating elements and a cis-repressing element (RLE) that is required for H3K27me3 marking were characterized. Remarkably, insertion of the RLE cis-element into pF3H, an unrelated promoter, is sufficient for repressing its transcriptional activity in different tissues. Besides improving our understanding of LEC2 regulation, this study provides important new insights into the mechanisms underlying H3K27me3 deposition and PRC2 recruitment at a specific locus in plants.
authors.affiliation: Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318 Institut National de la Recherche Agronomique-Agro-ParisTech, Saclay Plant Sciences, 78026 Versailles cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Berger
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubreucq
authors.forename: François
authors.initials: F
authors.lastname: Roudier
authors.forename: Christian
authors.initials: C
authors.lastname: Dubos
authors.forename: Loïc
authors.initials: L
authors.lastname: Lepiniec
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D038621:Regulatory Sequences, Ribonucleic Acid; D012639:Seeds / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; C504240:LEC2 protein, Arabidopsis; D038621:Regulatory Sequences, Ribonucleic Acid; D014157:Transcription Factors; D008239:Lysine
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