title: Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy.
abstract: We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological samples, showing, in some cases, more than one order of magnitude improvement in the total collected signal intensity.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Aviles-Espinosa
authors.forename: Jordi
authors.initials: J
authors.lastname: Andilla
authors.forename: Rafael
authors.initials: R
authors.lastname: Porcar-Guezenec
authors.forename: Omar E
authors.initials: OE
authors.lastname: Olarte
authors.forename: Marta
authors.initials: M
authors.lastname: Nieto
authors.forename: Xavier
authors.initials: X
authors.lastname: Levecq
authors.forename: David
authors.initials: D
authors.lastname: Artigas
authors.forename: Pablo
authors.initials: P
authors.lastname: Loza-Alvarez
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