title: Hyaluronan and fibrin biomaterial as scaffolds for neuronal differentiation of adult stem cells derived from adipose tissue and skin.
abstract: Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.
authors.affiliation: Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Via G. Colombo 3, 35100 Padova, Italy; E-Mails:  Chiaragardin@unipd.it  (C.G.);  letia.ferroni@unipd.it  (L.F.);  elisa.nalessoo@unipd.it  (E.N.).
authors.forename: Chiara
authors.initials: C
authors.lastname: Gardin
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vindigni
authors.forename: Eriberto
authors.initials: E
authors.lastname: Bressan
authors.forename: Letizia
authors.initials: L
authors.lastname: Ferroni
authors.forename: Elisa
authors.initials: E
authors.lastname: Nalesso
authors.forename: Alessandro Della
authors.initials: AD
authors.lastname: Puppa
authors.forename: Domenico
authors.initials: D
authors.lastname: D'Avella
authors.forename: Diego
authors.initials: D
authors.lastname: Lops
authors.forename: Paolo
authors.initials: P
authors.lastname: Pinton
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D043171:Chromosomal Instability; D015718:Fibrin Tissue Adhesive / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007621:Karyotyping; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D009419:Nerve Tissue Proteins; D006820:Hyaluronic Acid
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