title: Serum proteome analysis of vivax malaria: An insight into the disease pathogenesis and host immune response.
abstract: Vivax malaria is the most widely distributed human malaria resulting in 80-300 million clinical cases every year. It causes severe infection and mortality but is generally regarded as a benign disease and has not been investigated in detail. The present study aimed to perform human serum proteome analysis in a malaria endemic area in India to identify potential serum biomarkers for vivax malaria and understand host response. The proteomic analysis was performed on 16 age and gender matched subjects (vivax patients and control) in duplicate. Protein extraction protocols were optimized for large coverage of the serum proteome and to obtain high-resolution data. Identification of 67 differentially expressed and statistically significant (Student's t-test; p<0.05) protein spots was established by MALDI-TOF/TOF mass spectrometry. Many of the identified proteins such as apolipoprotein A and E, serum amyloid A and P, haptoglobin, ceruloplasmin, and hemopexin are interesting from a diagnostic point of view and could further be studied as potential serum biomarkers. The differentially expressed serum proteins in vivax malaria identified in this study were subjected to functional pathway analysis using multiple software, including Ingenuity Pathway Analysis (IPA), Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) functional annotation tool for better understanding of the biological context of the identified proteins, their involvement in various physiological pathways and association with disease pathogenesis. Functional pathway analysis of the differentially expressed proteins suggested the modulation of multiple vital physiological pathways, including acute phase response signaling, complement and coagulation cascades, hemostasis and vitamin D metabolism pathway due to this parasitic infection. This article is part of a Special Issue entitled: Proteomics: The clinical link.
authors.affiliation: Wadhwani Research Center for Biosciences and Bioengineering, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Sandipan
authors.initials: S
authors.lastname: Ray
authors.forename: Karthik S
authors.initials: KS
authors.lastname: Kamath
authors.forename: Rajneesh
authors.initials: R
authors.lastname: Srivastava
authors.forename: Dinesh
authors.initials: D
authors.lastname: Raghu
authors.forename: Kishore
authors.initials: K
authors.lastname: Gollapalli
authors.forename: Rekha
authors.initials: R
authors.lastname: Jain
authors.forename: Shipra V
authors.initials: SV
authors.lastname: Gupta
authors.forename: Sayantan
authors.initials: S
authors.lastname: Ray
authors.forename: Santosh
authors.initials: S
authors.lastname: Taur
authors.forename: Snigdha
authors.initials: S
authors.lastname: Dhali
authors.forename: Nithya
authors.initials: N
authors.lastname: Gogtay
authors.forename: Urmila
authors.initials: U
authors.lastname: Thatte
authors.forename: Rapole
authors.initials: R
authors.lastname: Srikanth
authors.forename: Swati
authors.initials: S
authors.lastname: Patankar
authors.forename: Sanjeeva
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006790:Host-Parasite Interactions / Q000502:physiology; D006801:Humans; D007109:Immunity / Q000502:physiology*; D016780:Malaria, Vivax / Q000097:blood* / Q000209:etiology* / Q000276:immunology* / Q000378:metabolism; D008297:Male; D010966:Plasmodium vivax / Q000502:physiology; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012698:Serologic Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D020543:Proteome