title: CCR5 promoter haplotype transcription complex characterization.
abstract: CC chemokine receptor 5 (CCR5) is a major coreceptor for cell entry of human immunodeficiency virus (HIV); its expression is highly associated with virus replication and susceptibility. Single nucleotide polymorphisms (SNPs) in the CCR5 promoter play a critical role in CCR5 transcriptional regulation. HHA and HHE represent two contrasting haplotypes of CCR5 with only two base pair differences in the promoter. Identifying the transcription factors (TFs) that differentially bind to the polymorphic sites (the SNPs) in CCR5 haplotypes aids understanding HIV transmission/pathogenesis. Promoter trapping and two-dimensional southwestern blot analysis, to purify transcription complex and identify the differential TFs binding profile, is combined with HPLC-ESI-MS/MS, to determine those proteins specifically bound to one haplotype. This strategy reveals clear differences in haplotype-TF binding and has great promise for investigating how the CCR5 haplotypes may affect HIV-AIDS (acquired immune deficiency syndrome) susceptibility or disease progression.
authors.affiliation: Department of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA. daifeng.jiang@utsa.edu
authors.forename: Daifeng
authors.initials: D
authors.lastname: Jiang
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mummidi
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Ahuja
authors.forename: Harry W
authors.initials: HW
authors.lastname: Jarrett
mesh_terms: D015153:Blotting, Western; D019295:Computational Biology; D018450:Disease Progression; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000235:genetics* / Q000635:transmission; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D006239:Haplotypes; D006801:Humans; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D009841:Oligonucleotides; D019713:Receptors, CCR5; D014157:Transcription Factors
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