title: Detection of avian rotaviruses of groups A, D, F and G in diseased chickens and turkeys from Europe and Bangladesh.
abstract: Avian rotaviruses (AvRVs) represent a diverse group of intestinal viruses, which are suspected as the cause of several diseases in poultry with symptoms of diarrhoea, growth retardation or runting and stunting syndrome (RSS). To assess the distribution of AvRVs in chickens and turkeys, we have developed specific PCR protocols. These protocols were applied in two field studies investigating faecal samples or intestinal contents of diseased birds derived from several European countries and Bangladesh. In the first study, samples of 166 chickens and 33 turkeys collected between 2005 and 2008 were tested by PAGE and conventional RT-PCR and AvRVs were detected in 46.2%. In detail, 16.1% and 39.2% were positive for AvRVs of groups A or D, respectively. 11.1% of the samples contained both of them and only four samples (2.0%) contained rotaviruses showing a PAGE pattern typical for groups F and G. In the second study, samples from 375 chickens and 18 turkeys collected between 2009 and 2010 were analyzed using a more sensitive group A-specific and a new group D-specific real-time RT-PCR. In this survey, 85.0% were AvRV-positive, 58.8% for group A AvRVs, 65.9% for group D AvRVs and 38.9% for both of them. Although geographical differences exist, the results generally indicate a very high prevalence of group A and D rotaviruses in chicken and turkey flocks with cases of diarrhoea, growth retardation or RSS. The newly developed diagnostic tools will help to investigate the epidemiology and clinical significance of AvRV infections in poultry.
authors.affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany. peter.otto@fli.bund.de
authors.forename: Peter H
authors.initials: PH
authors.lastname: Otto
authors.forename: Muzahed Uddin
authors.initials: MU
authors.lastname: Ahmed
authors.forename: Helmut
authors.initials: H
authors.lastname: Hotzel
authors.forename: Patrycja
authors.initials: P
authors.lastname: Machnowska
authors.forename: Jochen
authors.initials: J
authors.lastname: Reetz
authors.forename: Bernhard
authors.initials: B
authors.lastname: Roth
authors.forename: Eva
authors.initials: E
authors.lastname: Trojnar
authors.forename: Reimar
authors.initials: R
authors.lastname: Johne
mesh_terms: D000818:Animals; D001459:Bangladesh; D002645:Chickens; D005060:Europe; D006801:Humans; D010802:Phylogeny; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D014422:Turkeys
references.citation: Vet Rec. 1984 Mar 3;114(9):219
references.pmid: 6730283
references.citation: Virology. 2009 Apr 10;386(2):334-43
references.pmid: 19249805
references.citation: Avian Dis. 1985 Jul-Sep;29(3):798-811
references.pmid: 3000337
references.citation: Epidemiol Infect. 1988 Jun;100(3):481-92
references.pmid: 2837407
references.citation: Virology. 2011 Apr 10;412(2):384-91
references.pmid: 21329955
references.citation: Avian Dis. 1987 Jan-Mar;31(1):89-98
references.pmid: 3034232
references.citation: Avian Dis. 2010 Mar;54(1):16-21
references.pmid: 20408393
references.citation: Avian Pathol. 1979 Jul;8(3):239-47
references.pmid: 18770449
references.citation: J Vet Med B Infect Dis Vet Public Health. 2002 Mar;49(2):77-81
references.pmid: 12002423
references.citation: Am J Vet Res. 1987 Jun;48(6):977-83
references.pmid: 3037952
references.citation: Arch Virol. 1999;144(12):2467-73
references.pmid: 10664399
references.citation: Adv Virus Res. 1996;46:69-95
references.pmid: 8824698
references.citation: Comp Immunol Microbiol Infect Dis. 1997 Jun;20(3):205-10
references.pmid: 9280387
references.citation: Virology. 2009 Apr 10;386(2):325-33
references.pmid: 19246068
references.citation: Avian Dis. 1987 Apr-Jun;31(2):376-9
references.pmid: 3039968
references.citation: Res Vet Sci. 1987 Jul;43(1):130-1
references.pmid: 2820007
references.citation: Avian Pathol. 1986;15(1):149-59
references.pmid: 18766513
references.citation: Nihon Juigaku Zasshi. 1990 Dec;52(6):1319-21
references.pmid: 1962834
references.citation: J Vet Med Sci. 1991 Jun;53(3):479-86
references.pmid: 1653040
references.citation: Arch Virol. 1984;81(1-2):113-21
references.pmid: 6331344
references.citation: Avian Dis. 1987 Apr-Jun;31(2):272-6
references.pmid: 3039964
references.citation: Avian Dis. 1986 Jul-Sep;30(3):551-6
references.pmid: 3021101
references.citation: Arch Virol. 2011 Aug;156(8):1397-413
references.pmid: 21597953
references.citation: J Clin Microbiol. 1986 Apr;23(4):695-9
references.pmid: 3009539
references.citation: Avian Dis. 2007 Sep;51(3):681-4
references.pmid: 17992926
references.citation: Vet Rec. 1992 Mar 28;130(13):273
references.pmid: 1316665
references.citation: Avian Dis. 2006 Sep;50(3):411-8
references.pmid: 17039842
references.citation: J Virol. 2010 Oct;84(19):10254-65
references.pmid: 20631147
references.citation: Avian Dis. 2007 Sep;51(3):674-80
references.pmid: 17992925
references.citation: Vet Rec. 1978 Sep 30;103(14):319-20
references.pmid: 213873
references.citation: Avian Pathol. 1986;15(4):719-30
references.pmid: 18766573