title: Protein kinase D regulates the human cardiac L-type voltage-gated calcium channel through serine 1884.
abstract: Protein kinase D (PKD) regulates the activity of the L-type calcium channel in rat ventricular cardiomyocytes. However, the functional target residues of PKD on the L-type calcium channel remain to be identified. Our aim was to identify the functional phosphorylation sites of PKD on the human L-type calcium channel. The pore subunit of the human CaV1.2 (hCaV1.2) was stably expressed in HEK293 cells. Both the expression of a dominant-negative mutant of PKD and the mutation of serine 1884 but not serine 1930, putative targets of PKD, strongly reduced L-type calcium currents and single channel activity without affecting the channel's expression at the plasma membrane. Our results suggest that serine 1884 is essential for the regulation of hCaV1.2 by PKD.
authors.affiliation: Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Aita
authors.forename: Nagomi
authors.initials: N
authors.lastname: Kurebayashi
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Hirose
authors.forename: Andrés D
authors.initials: AD
authors.lastname: Maturana
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D020746:Calcium Channels, L-Type / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D012694:Serine
chemical_list: D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C); D012694:Serine; C089212:protein kinase D; D011493:Protein Kinase C