title: A simple elastic membrane-based microfluidic chip for the proliferation and differentiation of mesenchymal stem cells under tensile stress.
abstract: This work presents a simple membrane-based microfluidic chip for the investigation of proliferation and differentiation of mesenchymal stem cells (MSCs) under mechanical stimuli. The cyclic tensile stress was generated by the deformation of elastic PDMS membrane sandwiched between the two layer microfluidic chip via actuated negative pressure, and the cultured MSCs on membrane were subjected to different orders of tensile stress. The results suggest that mechanical stimuli are attributed to the different phenomena of MSCs in cell proliferation and differentiation. The higher tensile stress (>3.5) promoted obvious proliferation, osteogenesis and reduced adipogenesis in MSCs, indicating the possible regulative role of tensile stress in modifying the osteogenesis/adipogenesis balance in the development of tissue organ.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P R China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Gao
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Huiyu
authors.initials: H
authors.lastname: Tong
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D004867:Equipment Design; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010012:Osteogenesis / Q000502:physiology; D013314:Stress, Mechanical; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D003470:Culture Media; D000469:Alkaline Phosphatase