title: Application of hollow fiber-supported liquid-phase microextraction coupled with HPLC for the determination of guaifenesin enantiomer-protein binding.
abstract: A hollow fiber liquid-phase microextraction technique coupled with high-performance liquid chromatography with fluorescence detection was employed for determination and evaluation of the binding characteristics of drugs to bovine serum albumin (BSA). Enantiomers of guaifenesin (an expectorant drug) were investigated as a model system. After optimization of some influencing parameters on microextraction, the proposed method was used for calculation of the target drug distribution coefficient between n-octanol and the buffer solution as well as study of drug-BSA binding in physiological conditions. The developed method shows a new, improved and simple procedure for determination of free drug concentration in biological fluids and the extent of drug-protein binding.
authors.affiliation: Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hatami
authors.forename: Khalil
authors.initials: K
authors.lastname: Farhadi
mesh_terms: D020003:1-Octanol / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006140:Guaifenesin / Q000737:chemistry* / Q000378:metabolism*; D057230:Limit of Detection; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D008956:Models, Chemical; D009994:Osmolar Concentration; D011485:Protein Binding; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D012710:Serum Albumin, Bovine; D006140:Guaifenesin; D020003:1-Octanol