title: Crystallization and preliminary crystallographic studies of the HA3 subcomponent of the type B botulinum neurotoxin complex.
abstract: The haemagglutinin subcomponent HA3 of the type B botulinum neurotoxin complex, which is important in toxin absorption from the gastrointestinal tract, has been expressed, purified and subsequently crystallized in two crystal forms at different pH values. Form I belonged to space group R32, with unit-cell parameters a = b = 357.4, c = 249.5 Å, α = β = 90, γ = 120°. Form II belonged to space group I4(1)32, with unit-cell parameters a = b = c = 259.0 Å, α = β = γ = 90°. Diffraction data were collected from these crystals to a resolution of 3.0 Å for both form I and form II.
authors.affiliation: Graduate School of Science and Technology, Department of Biomolecular Engineering, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Nishimura
authors.forename: Kengo
authors.initials: K
authors.lastname: Kitadokoro
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takegahara
authors.forename: Yo
authors.initials: Y
authors.lastname: Sugawara
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Matsumura
authors.forename: Hajime
authors.initials: H
authors.lastname: Karatani
authors.forename: Yukako
authors.initials: Y
authors.lastname: Fujinaga
mesh_terms: D001905:Botulinum Toxins / Q000737:chemistry*; D019274:Botulinum Toxins, Type A; D003014:Clostridium botulinum / Q000737:chemistry*; D003461:Crystallography; D018360:Crystallography, X-Ray
chemical_list: C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A
references.citation: Physiol Rev. 2000 Apr;80(2):717-66
references.pmid: 10747206
references.citation: J Cell Biol. 2010 May 17;189(4):691-700
references.pmid: 20457762
references.citation: Microbiology (Reading). 2003 Dec;149(Pt 12):3361-3370
references.pmid: 14663070
references.citation: Cell Microbiol. 2008 Feb;10(2):355-64
references.pmid: 17868282
references.citation: J Mol Biol. 2008 Feb 22;376(3):854-67
references.pmid: 18178224
references.citation: J Mol Biol. 1991 Sep 5;221(1):31-4
references.pmid: 1656050
references.citation: J Biol Chem. 2007 Aug 24;282(34):24777-83
references.pmid: 17581814
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Microbiology (Reading). 2009 Jan;155(Pt 1):35-45
references.pmid: 19118344
references.citation: J Mol Biol. 2009 Jan 30;385(4):1193-206
references.pmid: 19071137
references.citation: Pharmacol Ther. 1982;19(2):165-94
references.pmid: 6763707
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Biochemistry. 2005 Jul 19;44(28):9574-80
references.pmid: 16008342