title: Human Cell Surface Receptors as Molecular Imaging Candidates for Metastatic Prostate Cancer.
abstract: Existing clinical imaging procedures lack sensitivity and specificity in detecting early prostate cancer bone metastatic lesions. In this study, we developed a highly reproducible bone metastasis xenograft model and identified possible molecular imaging candidates for detecting early bone metastatic lesions. Bone trophic human prostate cells (PC-3B1) were isolated and characterized for their ability to reach bone after intracardiac injection into SCID mice. The appearances of skeletal metastases were evaluated using digital radiographic imaging and confirmed by necropsy and histology. The PC-3B1 cells retain a bone homing phenotype after long term propagation in tissue culture and exhibit progressive bone lesions within 3 weeks following intracardiac injection. Comparative transcription signatures of PC-3 and PC-3B1 cells were determined using a cancer specific microarray and confirmed by RT-PCR analysis. The analysis identified increased expression of four cell surface molecules in PC-3B1 cells that may be suitable as molecular imaging candidates to detect bone micro metastases.
authors.affiliation: Department of Pharmacology, University of Arizona, Tucson, AZ 85724.
authors.forename: Isis C
authors.initials: IC
authors.lastname: Sroka
authors.forename: Gerald D
authors.initials: GD
authors.lastname: Pond
authors.forename: Raymond B
authors.initials: RB
authors.lastname: Nagle
authors.forename: Frank
authors.initials: F
authors.lastname: Porreca
authors.forename: Tamara
authors.initials: T
authors.lastname: King
authors.forename: Gary
authors.initials: G
authors.lastname: Pestano
authors.forename: Bernard W
authors.initials: BW
authors.lastname: Futscher
authors.forename: Jaime M
authors.initials: JM
authors.lastname: Gard
authors.forename: Janice
authors.initials: J
authors.lastname: Riley
authors.forename: Anne E
authors.initials: AE
authors.lastname: Cress
references.citation: Hematol Oncol. 1993 Jan-Feb;11(1):35-42
references.pmid: 8325625
references.citation: Int J Cancer. 1998 Sep 11;77(6):887-94
references.pmid: 9714059
references.citation: ChemMedChem. 2008 Sep;3(9):1311-5
references.pmid: 18613203
references.citation: Neoplasia. 2006 Mar;8(3):214-22
references.pmid: 16611415
references.citation: Neoplasia. 2005 Oct;7(10):904-11
references.pmid: 16242073
references.citation: Clin Exp Metastasis. 2003;20(4):327-34
references.pmid: 12856720
references.citation: Invest Urol. 1979 Jul;17(1):16-23
references.pmid: 447482
references.citation: Radiology. 2007 Nov;245(2):333-56
references.pmid: 17940297
references.citation: Cell Signal. 2008 Jun;20(6):1061-72
references.pmid: 18302981
references.citation: Cancer Res. 2009 Jun 15;69(12):5007-14
references.pmid: 19491258
references.citation: J Biol Chem. 2001 Jul 13;276(28):26099-106
references.pmid: 11359780
references.citation: Cancer Res. 1999 Feb 15;59(4):781-6
references.pmid: 10029062
references.citation: Stem Cells. 2007 Apr;25(4):844-51
references.pmid: 17185613
references.citation: Exp Cell Res. 2007 Apr 1;313(6):1080-9
references.pmid: 17303120
references.citation: Stem Cells. 2006 May;24(5):1254-64
references.pmid: 16410389
references.citation: J Nucl Med. 2008 Mar;49(3):414-21
references.pmid: 18287261
references.citation: Mol Imaging. 2007 Jul-Aug;6(4):277-88
references.pmid: 17711783
references.citation: Clin Cancer Res. 2006 Oct 15;12(20 Pt 2):6213s-6216s
references.pmid: 17062703
references.citation: Adv Cancer Res. 2008;100:35-83
references.pmid: 18620092
references.citation: Cancer Res. 1994 May 15;54(10):2577-81
references.pmid: 8168083
references.citation: Int J Oncol. 2008 Jul;33(1):17-24
references.pmid: 18575746
references.citation: Nature. 2005 Jul 28;436(7050):518-24
references.pmid: 16049480
references.citation: Neoplasia. 2007 May;9(5):406-17
references.pmid: 17534446
references.citation: Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6246-51
references.pmid: 11983914
references.citation: Development. 1994 Dec;120(12):3657-66
references.pmid: 7529679
references.citation: Cancer Res. 1988 Dec 1;48(23):6876-81
references.pmid: 3180096
references.citation: Neoplasia. 2002 May-Jun;4(3):243-54
references.pmid: 11988844
references.citation: Leukemia. 2008 May;22(5):941-50
references.pmid: 18305549
references.citation: Cytokine Growth Factor Rev. 2006 Aug;17(4):295-304
references.pmid: 16737840
references.citation: Trends Mol Med. 2007 May;13(5):183-91
references.pmid: 17403616
references.citation: Leuk Lymphoma. 1996 Oct;23(3-4):297-303
references.pmid: 9031110
references.citation: Curr Opin Urol. 2008 Jan;18(1):71-7
references.pmid: 18090494
references.citation: Magn Reson Imaging. 2004 Jul;22(6):843-50
references.pmid: 15234453
references.citation: Cancer Res. 1953 Jun;13(6):397-404
references.pmid: 13051750
references.citation: Clin Cancer Res. 2006 Mar 1;12(5):1431-40
references.pmid: 16533765
references.citation: Biochem Biophys Res Commun. 2004 Jul 30;320(3):712-6
references.pmid: 15240106
references.citation: Nat Clin Pract Urol. 2005 Sep;2(9):434-42
references.pmid: 16474681
references.citation: J Cell Biochem. 2008 Oct 1;105(2):370-80
references.pmid: 18636554
references.citation: Radiographics. 2007 Jan-Feb;27(1):63-75; discussion 75-7
references.pmid: 17234999
references.citation: Bone. 2006 Jul;39(1):27-34
references.pmid: 16459153
references.citation: Am J Pathol. 2002 Mar;160(3):1143-53
references.pmid: 11891210
references.citation: J Cell Physiol. 1998 Dec;177(3):426-38
references.pmid: 9808151