title: Vascularized bone marrow transplantation model in rats as an alternative to conventional cellular bone marrow transplantation: preliminary results.
abstract: The aim of the study was to follow the development of microchimerism after allogeneic vascularized bone marrow transplantation (VBMT) versus conventional bone marrow transplantation (BMT). In one group, a VBMT model consisted of donor Brown Norway rat hind limb heterotopic transplanted on recipient Lewis rats. An intravenous infusion of donor bone marrow cells in suspension equivalent to that grafted in the vascularized femur limb was administered intravenously to recipient rats in the second group. Cellular microchimerism was investigated in recipients of VBMT versus BMT. Donor-derived cells could be detected in VBMT recipients at 30 and 60 days but not in recipients of intravenous suspension of BMC. VBMT provides a theoretical alternative to conventional cellular bone marrow transplantation by addressing crucial clinical problems such as failure of engraftment or graft-versus-host disease.
authors.affiliation: Carol Davila Bucharest Medical University, Bucharest, Romania. dragoszamfirescu@gmail.com
authors.forename: D
authors.initials: D
authors.lastname: Zamfirescu
authors.forename: C
authors.initials: C
authors.lastname: Popovicu
authors.forename: A
authors.initials: A
authors.lastname: Stefanescu
authors.forename: A
authors.initials: A
authors.lastname: Bularda
authors.forename: M
authors.initials: M
authors.lastname: Popescu
authors.forename: I
authors.initials: I
authors.lastname: Zegrea
authors.forename: M
authors.initials: M
authors.lastname: Lanzetta
authors.forename: I
authors.initials: I
authors.lastname: Lascar
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods*; D049109:Cell Proliferation; D046528:Chimerism; D016572:Cyclosporine / Q000494:pharmacology; D006086:Graft vs Host Disease; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D023421:Models, Animal; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013234:Stem Cells / Q000166:cytology
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine