title: Repair of rabbit ulna segmental bone defect using freshly isolated adipose-derived stromal vascular fraction.
abstract: OBJECTIVE
  Stromal vascular fractions (SVF) from adipose tissue have heterogeneous cell populations, and include multipotent adipose-derived stem cells. The advantages of using of SVF include the avoidance of an additional culture period, a reduced risk of extensive cell contamination, and cost-effectiveness.
  
  
  METHODS
  Unilateral 20-mm mid-diaphyseal segmental defects in rabbit ulna were treated with one of the following: polylactic glycolic acid (PLGA) scaffold alone (group 1, control), a PLGA scaffold with undifferentiated SVF cells (group 2), or a PLGA scaffold with osteogenically differentiated SVF cells (group 3). At 8 weeks after implantation, five rabbits in each treatment group were killed to assess bone defect healing by plain radiography, quantitative microcomputed tomography and histology.
  
  
  RESULTS
  The SVF cells were well grown on PLGA scaffolds and expressed type I collagen and alkaline phosphatase (ALP). The intensity of ALP and OPN gene expressions in osteogenic medium culture were increased from 14 days to 28 days. In vivo evaluations at 8 weeks showed that treatment of SVF cells with or without osteogenic differentiation resulted in more bone formation in the critically sized segmental defects than PLGA scaffold alone. Osteogenically differentiated SVF cells significantly enhanced bone healing compared with undifferentiated SVF cells.
  
  
  CONCLUSIONS
  Adipose-derived stromal SVF showed osteogenic potential in vitro. Accordingly, SVF could provide a cell source for bone tissue engineering. However, treatment with uncultured SVF cells on bone healing was not satisfactory in the in vivo animal model.
authors.affiliation: Department of Pathology, Korea University Guro Hospital ,  Seoul ,  Republic of Korea.
authors.forename: Aeree
authors.initials: A
authors.lastname: Kim
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Hae-Ryong
authors.initials: HR
authors.lastname: Song
authors.forename: Won-Hwa
authors.initials: WH
authors.lastname: Kang
authors.forename: Hak-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hong-Chul
authors.initials: HC
authors.lastname: Lim
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Bae
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D011100:Polyglycolic Acid / Q000378:metabolism / Q000627:therapeutic use; D011817:Rabbits; D033581:Stem Cell Transplantation / Q000379:methods*; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014457:Ulna / Q000293:injuries / Q000473:pathology / Q000601:surgery*
chemical_list: D024042:Collagen Type I; D003470:Culture Media; D011100:Polyglycolic Acid; D000469:Alkaline Phosphatase