title: Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair.
abstract: Low-level laser irradiation (LLLI) and recombinant human bone morphogenetic protein type 2 (rhBMP-2) have been used to stimulate bone formation. LLLI stimulates proliferation of osteoblast precursor cells and cell differentiation and rhBMP-2 recruits osteoprogenitor cells to the bone healing area. This in vivo study evaluated the effects of LLLI and rhBMP-2 on the bone healing process in rats. Critical bone defects were created in the parietal bone in 42 animals, and the animals were divided into six treatment groups: (1) laser, (2) 7 μg of rhBMP-2, (3) laser and 7 μg of rhBMP-2, (4) 7 μg of rhBMP-2/monoolein gel, (5) laser and 7 μg rhBMP-2/monoolein gel, and (6) critical bone defect controls. A gallium-aluminum-arsenide diode laser was used (wavelength 780 nm, output power 60 mW, beam area 0.04 cm(2), irradiation time 80 s, energy density 120 J/cm(2), irradiance 1.5 W/cm(2)). After 15 days, the calvarial tissues were removed for histomorphometric analysis. Group 3 defects showed higher amounts of newly formed bone (37.89%) than the defects of all the other groups (P < 0.05). The amounts of new bone in defects of groups 1 and 4 were not significantly different from each other (24.00% and 24.75%, respectively), but were significantly different from the amounts in the other groups (P < 0.05). The amounts of new bone in the defects of groups 2 and 5 were not significantly different from each other (31.42% and 31.96%, respectively), but were significantly different from the amounts in the other groups (P < 0.05). Group 6 defects had 14.10% new bone formation, and this was significantly different from the amounts in the other groups (P < 0.05). It can be concluded that LLLI administered during surgery effectively accelerated healing of critical bone defects filled with pure rhBMP-2, achieving a better result than LLLI alone or the use of rhBMP-2 alone.
authors.affiliation: Department of Morphology, Stomatology and Physiology, School of Dentistry at Ribeirão Preto, University of São Paulo, Avenida do Café, s/n - Bairro - Monte Alegre, 14040-904, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Anderson Paim
authors.initials: AP
authors.lastname: Rosa
authors.forename: Luiz Gustavo
authors.initials: LG
authors.lastname: de Sousa
authors.forename: Simone Cecilio Hallak
authors.initials: SC
authors.lastname: Regalo
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
authors.forename: Ana Paula Amorim
authors.initials: AP
authors.lastname: Barbosa
authors.forename: Dimitrius Leonardo
authors.initials: DL
authors.lastname: Pitol
authors.forename: Richard Honorato
authors.initials: RH
authors.lastname: de Oliveira
authors.forename: Paulo Batista
authors.initials: PB
authors.lastname: de Vasconcelos
authors.forename: Fernando José
authors.initials: FJ
authors.lastname: Dias
authors.forename: Daniela Thomazatti
authors.initials: DT
authors.lastname: Chimello
authors.forename: Selma
authors.initials: S
authors.lastname: Siéssere
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects* / Q000528:radiation effects*; D005260:Female; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage; D012886:Skull / Q000187:drug effects / Q000293:injuries / Q000473:pathology / Q000528:radiation effects
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins
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