title: Microscopic comparison of topical use of Mitomycin C and Fluorouracil on cold knife myringotomy.
abstract: Objective/hypothesis A comparison of the histopathological effect of topical use of Mitomycin C and 5-Fluorouracil in preventing myringotomy closure in rats.
  
  
  METHODS
  clinical trial. Methods and materials The study was performed on 43 rats that were divided into three groups. Study groups (A and B) and control group (C) after bilateral cold-knife myringotomy, we applied Mitomycin C (MMC) 4mg/ml to group A, 5-Fluouracil (5FU) 50mg/ml to group B, and normal saline to group C. An examination of all ears of rats was carried out by otoscope on days 0, 1, 3, 5, 7, and then every five days up to 70 days. Each day's closed myringotomies of all groups were examined. Results The mean of post myringotomy opening time was 37, 16, and 12 days respectively in MMC, 5FU, and saline. Patency duration of MMC group was significantly long (p<0.0001), but in histopatholgical examinations, sclerosis of tympanic membrane in MMC group showed the highest patency duration (p<0.0001). Conclusion Mitomycin C significantly prolonged the duration of myringotomy patency time - longer than 5-Fluouracil and saline but with the adverse effects of tympanic membrane fibrosis.
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mosoud
authors.initials: M
authors.lastname: NaderPour
authors.forename: Yalda Jabbari
authors.initials: YJ
authors.lastname: Moghaddam
authors.forename: Ali
authors.initials: A
authors.lastname: Peirovifar
authors.forename: Rauof
authors.initials: R
authors.lastname: Mollajavadi
authors.forename: Mehran Mesgari
authors.initials: MM
authors.lastname: Abbasi
authors.forename: Daryoush
authors.initials: D
authors.lastname: Mohajeri
mesh_terms: D000287:Administration, Topical; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D008859:Microscopy, Polarization / Q000379:methods; D008876:Middle Ear Ventilation / Q000009:adverse effects / Q000379:methods; D016685:Mitomycin / Q000494:pharmacology*; D038781:Otoscopy / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D014432:Tympanic Membrane / Q000187:drug effects / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016685:Mitomycin; D005472:Fluorouracil