title: The expression of CD25, CD11b, SWC1, SWC7, MHC-II, and family of CD45 molecules can be used to characterize different stages of γδ T lymphocytes in pigs.
abstract: The expression of selected molecules was chosen to study porcine γδ lymphocytes and their CD2/CD8 subsets in different lymphoid organs in vivo and in vitro. Results indicate that many γδ T cells can constitutively express CD25 and MHC-II and that the frequency of γδ T cells positive for CD25, CD11b, SWC1 and SWC7 can be increased by stimulation. A diversified TCRδ repertoire was found inside CD25(+), CD11b(+), SWC1(-) and CD45RA(-) cells. Ontogenetic studies revealed various age and/or colonization dependency for expression of all studied molecules except of SWC7. Findings generally indicate that CD25 represent an activation molecule that probably marks a functionally distinct subsets, expression of CD11b is perhaps connected to early functions of naive γδ T cells in the periphery, SWC1 is lineage specific marker, SWC7 may represent an activation molecule with intrinsic or transient expression, and the expression of CD45RA/RC most likely defines naive and terminally differentiated cells.
authors.affiliation: Department of Immunology and Gnotobiology, Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic, Doly 183, 54922 Novy Hradek, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Stěpánová
authors.forename: Marek
authors.initials: M
authors.lastname: Sinkora
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D013552:Swine / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens