title: Fructose uptake in Bifidobacterium longum NCC2705 is mediated by an ATP-binding cassette transporter.
abstract: Recently, a putative ATP-binding cassette (ABC) transport system was identified in Bifidobacterium longum NCC2705 that is highly up-regulated during growth on fructose as the sole carbon source. Cloning and expression of the corresponding ORFs (bl0033-0036) result in efficient fructose uptake by bacteria. Sequence analysis reveals high similarity to typical ABC transport systems and suggests that these genes are organized as an operon. Expression of FruE is induced by fructose, ribose, or xylose and is able to bind these sugars with fructose as the preferred substrate. Our data suggest that BL0033-0036 constitute a high affinity fructose-specific ABC transporter of B. longum NCC2705. We thus suggest to rename the coding genes to fruEKFG and the corresponding proteins to FruE (sugar-binding protein), FruK (ATPase subunit), FruF, and FruG (membrane permeases). Furthermore, protein-protein interactions between the components of the transporter complex were determined by GST pulldown and Western blot analysis. This revealed interactions between the membrane subunits FruF and FruG with FruE, which in vivo is located on the external side of the membrane, and with the cytoplasmatic ATPase FruK. This is in line with the proposed model for bacterial ABC sugar transporters.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Shao
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; Institute of Microbiology and Biotechnology, University of Ulm, Ulm D-89069, Germany.
authors.forename: Zhongke
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, Ulm D-89069, Germany.
authors.forename: Daria
authors.initials: D
authors.lastname: Zhurina
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Dayang
authors.initials: D
authors.lastname: Zou
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. Electronic address: huangliuyuly@163.com.
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, Ulm D-89069, Germany. Electronic address: christian.riedel@uni-ulm.de.
authors.forename: Christian U
authors.initials: CU
authors.lastname: Riedel
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China. Electronic address: yuanjing6216@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001692:Biological Transport; D005632:Fructose / Q000172:deficiency / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D012266:Ribose / Q000378:metabolism; D013379:Substrate Specificity; D014994:Xylose / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D005632:Fructose; D012266:Ribose; D014994:Xylose
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