title: 1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum.
abstract: 1,3-Propanediol (1,3-PD) is widely used in polymer industry in production of polyethers, polyesters and polyurethanes. In this article, a study on 1,3-PD production and tolerance of Halanaerobium saccharolyticum subsp. saccharolyticum is presented. 1,3-PD production was optimized for temperature, vitamin B(12) and acetate concentration. The highest 1,3-PD concentrations and yields (0.6 mol/mol glycerol) were obtained at vitamin B₁₂ concentration 64 μg/l and an inverse correlation between 1,3-PD and hydrogen production was observed with varying vitamin B₁₂ concentrations. In the studied temperature range and initial acetate concentrations up to 10 g/l, no significant variations were observed in 1,3-PD production. High initial acetate (29-58 g/l) was observed to cause slight decrease in 1,3-PD concentrations produced but no effects on 1,3-PD yields (mol/mol glycerol). Initial 1,3-PD concentrations inhibited the growth of H. saccharolyticum subsp. saccharolyticum. When initial 1,3-PD concentration was raised from 1g/l to 57 g/l, a decrease of 12% to 75%, respectively, in the highest optical density was observed.
authors.affiliation: Tampere University of Technology, Department of Chemistry and Bioengineering, Tampere, Finland. anniina.kivisto@tut.fi
authors.forename: Anniina
authors.initials: A
authors.lastname: Kivistö
authors.forename: Ville
authors.initials: V
authors.lastname: Santala
authors.forename: Matti
authors.initials: M
authors.lastname: Karp
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D016931:Halobacteriales / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D013696:Temperature; D014805:Vitamin B 12 / Q000378:metabolism
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D006859:Hydrogen; D014805:Vitamin B 12; D005990:Glycerol; D019342:Acetic Acid