title: Enzymatic hydrolysis of microcrystalline cellulose in concentrated seawater.
abstract: This communication explores the use of seawater (1X) and concentrated seawater (2X and 4X) as reaction media for the enzyme-catalyzed depolymerization of cellulose. The commercially available Accellerase-1500® - a "cocktail" of different glycosidases - is able to depolymerize several amorphous celluloses and microcrystalline cellulose Avicel® in these reaction media, at slightly lower rates (ca. 90%) than those observed when reactions are performed in pure citrate buffer (control reactions). Remarkably, at concentrated seawater effluents enzymes also display significant rates of cellulose hydrolysis. Considering the expected increasing shortages in accessibility to fresh drinkable water, the herein-reported concept may provide novel inspiring leads for a smart use of resources in an environmentally-friendly and efficient manner, and for the genetic development of cellulases highly active and stable in concentrated seawater solutions.
authors.affiliation: Institut für Technische und Makromolekulare Chemie (ITMC), RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
authors.forename: Philipp M
authors.initials: PM
authors.lastname: Grande
authors.forename: Pablo Domínguez
authors.initials: PD
authors.lastname: de María
mesh_terms: D044602:Cellulases / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D003460:Crystallization; D006868:Hydrolysis; D010316:Particle Size; D012623:Seawater / Q000737:chemistry*
chemical_list: D002482:Cellulose; D044602:Cellulases