title: [Effect of epimedii folium processed with different refining temperatures and amounts of sheep's oil on kidney-yang deficiency rats].
abstract: OBJECTIVE
  To investigate the effects of refining temperatures and amounts of sheep's oil used in processing Epimedii Folium on Kedney-yang deficiency rats.
  
  
  METHODS
  The corticosterone was subcutaneous injected to establish the kidney yang deficiency rat model. With the temperatures and amounts of sheep's oil were 250 degrees C/30%, 120 degrees C/30% and 120 degrees C/20% respectively, the crude drug and three kinds of pressed Epimedii Folium were extracted by water and used as examined samples while total flavonoid of Epimedii Folium was used as positive control. After examined samples and control samples were intragastirc administrated, the pharmacologic action was analyzed.
  
  
  RESULTS
  As compared to crude drug, all of the aqueous extracts of processed Epimedii Folium have stronger effect of warming kidney and enhancing yang, especially the Epimedii Folium processed by sheep's oil with refining temperatures 120 degrees C and amounts of sheep's oil 30%. Its mechanism might be related to improving the insufficiency of hypothalamic-pituitary-adrenal-thymus (HPAT) axis suppression.
  
  
  CONCLUSIONS
  The refining temperature of sheep's oil can affect the quality of excipients and processed drugs. The results may be useful in explaining the mechanism of Epimedii Folium processing and establishment of pharmaceutical standard of sheep's oil used as processing excipients.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. lych@zzu.edu.cn
authors.forename: Yinchao
authors.initials: Y
authors.lastname: Li
authors.forename: Lanlan
authors.initials: L
authors.lastname: Huang
authors.forename: Yihong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Caifang
authors.initials: C
authors.lastname: Wang
authors.forename: Raorao
authors.initials: R
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Gu
authors.forename: Xuewu
authors.initials: X
authors.lastname: Liu
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Suihua
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007674:Kidney Diseases / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D009821:Oils / Q000008:administration & dosage*; D010913:Pituitary-Adrenal System / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D013678:Technology, Pharmaceutical; D016711:Yang Deficiency / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D004365:Drugs, Chinese Herbal; D009821:Oils; C046314:epimedii flavone