title: Preparation of acute living hippocampal slice from common marmoset (Callithrix jacchus) for synaptic function analysis.
abstract: We described the preparation of acute living slices from the hippocampus of the neonatal common marmoset (Callithrix jacchus). Slices from a temporal lobe section were prepared quickly using a rotary slicer. By this method, we successfully recorded field potentials, namely, pre-synaptic fiber volley and field excitatory post-synaptic potentials, from the hippocampal CA1 region with conventional electrophysiological techniques, and analyzed the indicators of synaptic function such as input-output curve. This study thus presents an efficient preparation method for acute living hippocampal slice from which synaptic function of the hippocampus in non-human primate can be analyzed.
authors.affiliation: Molecular Neuroimaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan. oh-nishi@nirs.go.jp
authors.forename: Arata
authors.initials: A
authors.lastname: Oh-Nishi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Obayashi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Minamimoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nagai
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hori
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suhara
mesh_terms: D000818:Animals; D002144:Callithrix; D004594:Electrophysiology / Q000379:methods*; D006624:Hippocampus / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*