title: A novel 2,3-xylenol-utilizing Pseudomonas isolate capable of degrading multiple phenolic compounds.
abstract: This work characterized a novel 2,3-xylenol-utilizing Pseudomonas isolate XQ23. From 16S rRNA phylogenetic analysis, XQ23 was found to be a member of the Pseudomonas putida group. Most of its physiological characteristics also shared similarities to P. putida. Phenols were catabolized by the meta-cleavage pathway. The dependence of the specific growth rate on 2,3-xylenol concentration could be well fitted by the Haldane model, with the maximum occurring at the concentration around 180 mg l(-1). Kinetic parameters indicated that XQ23 was sensitive to 2,3-xylenol and had low affinity. Three patterns, i.e. constant, linear decline, and allometric decline, were proposed to describe the biomass yields of phenols during bacterial degradation and XQ23 under 2,3-xylenol culturing conditions followed the allometric pattern. In a mineral-salts medium supplemented with 180 mg l(-1) of 2,3-xylenol as the sole carbon and energy source, over 40% of 2,3-xylenol was turned into CO(2) to provide energy by complete oxidization.
authors.affiliation: State Key Laboratory of Heavy Oil Processing and Center for Bioengineering & Biotechnology, China University of Petroleum, Qingdao 266555, China. zjxiao@upc.edu.cn
authors.forename: Zijun
authors.initials: Z
authors.lastname: Xiao
authors.forename: Fangfang
authors.initials: F
authors.lastname: Huo
authors.forename: Yunling
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiankun
authors.initials: X
authors.lastname: Zhu
authors.forename: Jian R
authors.initials: JR
authors.lastname: Lu
mesh_terms: D001673:Biodegradation, Environmental; D003198:Computer Simulation; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014992:Xylenes / Q000378:metabolism*
chemical_list: D010636:Phenols; D014992:Xylenes; C016834:3,5-xylenol