title: Effects of orange juice pH on survival, urease activity and DNA profiles of Yersinia enterocolitica and Yersinia pseudotuberculosis stored at 4 degree C.
abstract: The objective of this study was to determine the survival, growth rate and possible cellular adaptation mechanisms of Y. pseudotuberculosis and Y. enterocolitica in orange juice under different pH conditions. Yersinia was inoculated in orange juice with adjusted pH levels of 3.9, 4.0, and 7.0 and stored at 4 C for 3, 24, 72 and 168 hours (h). The inter-and intra-species variation is significant to the pH and time of incubation variables (p<0.05). At 3.9 pH the CFU (colony forming units) count decreased significantly.At pH 3.9 and 4.0, Y. enterocolitica and Y. pseudotuberculosis survived for at least 30 days and 15 days, respectively. Yersinia that survived under low pH in orange juice revealed enhanced urease activity within 12 h of incubation. The attachment gene (ail) could not be detected by PCR in Y. enterocolitica from undiluted sample incubated for 24 h or longer. Moreover, the FesI-restriction profile was altered when Y. pseudotuberculosis was stored at pH 4.0 orange juice for 7 days. These results indicate that Yersinia could survive and grow at low pH and the survival mechanisms could also enable the bacteria to survive the stomach pH barrier to cause enteric infection.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, AL 36088.
authors.forename: Woubit
authors.initials: W
authors.lastname: Abdela
authors.forename: Martha
authors.initials: M
authors.lastname: Graham
authors.forename: Habtemariam
authors.initials: H
authors.lastname: Tsegaye
authors.forename: Samuel
authors.initials: S
authors.lastname: Temesgen
authors.forename: Teshome
authors.initials: T
authors.lastname: Yehualaeshet
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