title: Crystallization and preliminary crystallographic analysis of an Ig-domain-encompassing fragment of the giant adhesion protein SiiE from Salmonella enterica.
abstract: Salmonella infections can be life-threatening. SiiE is a giant adhesion molecule of 5559 amino acids that is encoded in Salmonella pathogenicity island 4 (SPI4) and that promotes the initial contact between the pathogen and polarized epithelial cells in the intestine of the host. Starting from an engineered deletion version of SiiE (mini-SiiE; 97 kDa), limited proteolysis was used to reproducibly generate a 30 kDa fragment that readily crystallized. Mass spectrometry hints that this fragment spans the predicted Ig domains 50-52 of SiiE. Crystals of both native and selenomethionine-labelled protein could be obtained in space group C2 and diffraction data were recorded to a resolution of 1.85 Å.
authors.affiliation: Lehrstuhl für Biotechnik, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Karina U
authors.initials: KU
authors.lastname: Sturm
authors.forename: Martin H
authors.initials: MH
authors.lastname: Griessl
authors.forename: Carolin
authors.initials: C
authors.lastname: Wagner
authors.forename: Jörg
authors.initials: J
authors.lastname: Deiwick
authors.forename: Michael
authors.initials: M
authors.lastname: Hensel
authors.forename: Yves A
authors.initials: YA
authors.lastname: Muller
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D019779:Salmonella enterica / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins
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