title: Bioinformatics analysis on ORF1 protein of Torque teno virus (SANBAN isolate).
abstract: OBJECTIVE
  To analyze the sequence of ORF1 protein of Torque teno virus to prepare for the future hybrid experiments.
  
  
  METHODS
  The sequence of ORF1 protein of Torque teno virus was analyzed by bioinformatics using some web tools.
  
  
  RESULTS
  The most likely cleavage site was between position 14aa and 15aa and signal peptide may be position 1aa-14aa. Two possible transmembrane helices from inside to outside and three possible transmembrane helices from outside to inside were found. The position 509 (NKTN) was the potential N-glycosylation site. The speculative molecular weight of TTV ORF1 protein, which may be a kind of unstable protein was 88 705.7 Da. 1aa-91aa and 278aa-361aa were localized in non-regular secondary structure region.
  
  
  CONCLUSIONS
  TTV ORF1 protein may be a nuclear protein which contains two non-regular secondary structure region. 265aa to 486aa and 510aa to 679aa may be the two approciate fragments to construct the plasmids, which would be prepared for the future hybrid experiments to study the functional positions of the protein and the interactions between TTV and its hosts. Bioinformatics analysis would possibly make it easier to study the protein's function.
authors.affiliation: Department of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, 226001, Peoples Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jinling
authors.forename: Zhu
authors.initials: Z
authors.lastname: Dandan
authors.forename: Shen
authors.initials: S
authors.lastname: Pei
authors.forename: Sun
authors.initials: S
authors.lastname: Wei
authors.forename: Xiao
authors.initials: X
authors.lastname: Gengfu
authors.forename: Duan
authors.initials: D
authors.lastname: Yinong
authors.forename: Zhu
authors.initials: Z
authors.lastname: Ying
mesh_terms: D019295:Computational Biology / Q000379:methods*; D006801:Humans; D020407:Internet; D010957:Plasmids / Q000235:genetics; D022783:Torque teno virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: C446939:ORF1 protein, TT virus; D014764:Viral Proteins