title: Synthesis and biological evaluation of radiolabeled photosensitizer linked bovine serum albumin nanoparticles as a tumor imaging agent.
abstract: In this study, we reported on the synthesis and biological evaluation of radiolabeled fluorescent dye conjugated bovine serum albumin nanoparticles within the size range 190-210 nm. The bovine serum albumin nanoparticles (BSANPs) were prepared using a desolvation method, and chemical cross-linking was performed using gluteraldehyde. Furthermore, pheophorbide-a (PH-A) was loaded on the BSANPs. The results obtained from dynamic light scattering and electron microscopy have proved that nanoparticles are highly monodisperse and near-spherical shaped. The photo-physical properties of the PH-A-BSANPs were obtained using the spectrophotometric techniques. According to the results, PH-A and BSANPs show high non-covalent interaction. PH-A loaded nanoparticles were labeled with (99m)Tc and the radio-labeling efficiency was determined as 90 ± 1.2%. Biodistribution studies of (99m)Tc labeled PH-A-BSANPs and PH-A were carried out using female Albino Wistar rats, and (99m)Tc-PH-A-BSANPs showed a significantly higher uptake in the breast and uterus than (99m)Tc-PH-A. Cell culture study was carried out in MCF-7 cell line (human breast adenocarcinoma cell line). According to the cell culture studies, (99m)Tc-PH-A-BSANPs showed a higher uptake than (99m)Tc-PH-A. Moreover, PH-A-BSANPs demonstrated good photo-physical properties and BSANPs increased the uptake of PH-A on to the MCF-7 cell line. These results confirm that (99m)Tc labeled PH-A-BSANPs could be utilized for radioimaging.
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova TR-35100, Izmir, Turkey.
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozgur
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
authors.forename: Kasim
authors.initials: K
authors.lastname: Ocakoglu
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gunduz
authors.forename: Musteyde
authors.initials: M
authors.lastname: Yucebas
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers; D005260:Female; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles; D010316:Particle Size; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000493:pharmacokinetics* / Q000633:toxicity; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics*; D013056:Spectrophotometry, Ultraviolet; D013667:Technetium / Q000737:chemistry / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D011838:Radiation-Sensitizing Agents; D019275:Radiopharmaceuticals; D002734:Chlorophyll; D012710:Serum Albumin, Bovine; D013667:Technetium; C032623:pheophorbide a; D005976:Glutaral