title: Sphagnum mosses harbour highly specific bacterial diversity during their whole lifecycle.
abstract: Knowledge about Sphagnum-associated microbial communities, their structure and their origin is important to understand and maintain climate-relevant Sphagnum-dominated bog ecosystems. We studied bacterial communities of two cosmopolitan Sphagnum species, which are well adapted to different abiotic parameters (Sphagnum magellanicum, which are strongly acidic and ombrotrophic, and Sphagnum fallax, which are weakly acidic and mesotrophic), in three Alpine bogs in Austria by a multifaceted approach. Great differences between bacterial fingerprints of both Sphagna were found independently from the site. This remarkable specificity was confirmed by a cloning and a deep sequencing approach. Besides the common Alphaproteobacteria, we found a discriminative spectrum of bacteria; although Gammaproteobacteria dominated S. magellanicum, S. fallax was mainly colonised by Verrucomicrobia and Planctomycetes. Using this information for fluorescent in situ hybridisation analyses, corresponding colonisation patterns for Alphaproteobacteria and Planctomycetes were detected. Bacterial colonies were found in high abundances inside the dead big hyalocytes, but they were always connected with the living chlorocytes. Using multivariate statistical analysis, the abiotic factors nutrient richness and pH were identified to modulate the composition of Sphagnum-specific bacterial communities. Interestingly, we found that the immense bacterial diversity was transferred via the sporophyte to the gametophyte, which can explain the high specificity of Sphagnum-associated bacteria over long distances. In contrast to higher plants, which acquire their bacteria mainly from the environment, mosses as the phylogenetically oldest land plants maintain their bacterial diversity within the whole lifecycle.
authors.affiliation: Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Bragina
authors.forename: Christian
authors.initials: C
authors.lastname: Berg
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Cardinale
authors.forename: Andrey
authors.initials: A
authors.lastname: Shcherbakov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chebotar
authors.forename: Gabriele
authors.initials: G
authors.lastname: Berg
mesh_terms: D000818:Animals; D001317:Austria; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D008018:Life Cycle Stages; D044003:Sphagnopsida / Q000254:growth & development / Q000382:microbiology*; D053833:Wetlands
references.citation: ISME J. 2009 Sep;3(9):1105-15
references.pmid: 19554038
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Ecology. 2010 Sep;91(9):2716-26
references.pmid: 20957965
references.citation: Ecology. 2010 Aug;91(8):2356-65
references.pmid: 20836457
references.citation: PLoS One. 2007 Feb 14;2(2):e197
references.pmid: 17299583
references.citation: Appl Environ Microbiol. 2009 Aug;75(15):5111-20
references.pmid: 19502440
references.citation: ISME J. 2008 May;2(5):551-60
references.pmid: 18309356
references.citation: Environ Microbiol. 2007 Nov;9(11):2795-809
references.pmid: 17922763
references.citation: FEMS Microbiol Ecol. 2009 Apr;68(1):1-13
references.pmid: 19243436
references.citation: Appl Environ Microbiol. 2005 Oct;71(10):6193-8
references.pmid: 16204538
references.citation: Can J Microbiol. 2011 Mar;57(3):226-35
references.pmid: 21358764
references.citation: Appl Environ Microbiol. 2003 Jan;69(1):74-83
references.pmid: 12513979
references.citation: Microb Ecol. 2011 Aug;62(2):257-64
references.pmid: 21424277
references.citation: Appl Environ Microbiol. 2001 May;67(5):2354-9
references.pmid: 11319122
references.citation: ISME J. 2007 Oct;1(6):502-16
references.pmid: 18043652
references.citation: PLoS Comput Biol. 2006 Jul 21;2(7):e92
references.pmid: 16848637
references.citation: FEMS Microbiol Ecol. 2005 Jan 1;51(2):215-29
references.pmid: 16329870
references.citation: Bioinformatics. 2004 Sep 22;20(14):2317-9
references.pmid: 15073015
references.citation: Oecologia. 2009 Sep;161(3):481-90
references.pmid: 19593588
references.citation: Microb Ecol. 2008 Apr;55(3):415-24
references.pmid: 17690836
references.citation: Appl Environ Microbiol. 1998 Mar;64(3):922-9
references.pmid: 9501432
references.citation: Microb Ecol. 2001 Apr;41(3):252-263
references.pmid: 11391463
references.citation: Science. 2009 Nov 6;326(5954):810-1
references.pmid: 19892972
references.citation: ISME J. 2011 Jun;5(6):1061-6
references.pmid: 21160537
references.citation: Appl Environ Microbiol. 2001 Oct;67(10):4850-7
references.pmid: 11571193
references.citation: J Microbiol. 2004 Mar;42(1):9-13
references.pmid: 15357285
references.citation: Nature. 2005 Aug 25;436(7054):1153-6
references.pmid: 16121180
references.citation: FEMS Microbiol Ecol. 2007 Jul;61(1):38-53
references.pmid: 17484734
references.citation: Appl Environ Microbiol. 2004 Nov;70(11):6569-79
references.pmid: 15528520
references.citation: Nat Methods. 2010 May;7(5):335-6
references.pmid: 20383131
references.citation: Annu Rev Phytopathol. 2004;42:243-70
references.pmid: 15283667
references.citation: Appl Environ Microbiol. 1998 Dec;64(12):4870-6
references.pmid: 9835576