title: Fbl is not involved in the invasion of eukaryotic epithelial and endothelial cells by Staphylococcus lugdunensis.
abstract: For several Staphylococci, such as Staphylococcus aureus, Staphylococcus saprophyticus, and Staphylococcus epidermidis, invasion of eukaryotic cells has been described and this mechanism has been considered an important part of the infection process. The fibrinogen-binding protein (Fbl) of Staphylococcus lugdunensis, a homolog of the clumping factor A of S. aureus, has been described as fibrinogen-binding adhesin and might promote invasion of cells. We therefore characterized several clinical strains of S. lugdunensis in terms of whole cell fibrinogen and fibronectin binding and correlated these results with the invasion of epithelial and endothelial cells by S. lugdunensis. We described for the first time invasion of cells by S. lugdunensis. As invasion of cells by S. lugdunensis was only partly inhibited by cytochalasin D in contrast to a complete inhibition of invasion of cells by S. aureus, further invasion mechanisms are likely to be present in S. lugdunensis. In addition, the Fbl of S. lugdunensis is not involved in the invasion of cells as ruled out by an isogenic fbl mutant.
authors.affiliation: Institute for Hygiene and Microbiology, Department of Medical Microbiology, Ruhr-University of Bochum, Bochum, Germany. florian.szabados@rub.de
authors.forename: Florian
authors.initials: F
authors.lastname: Szabados
authors.forename: Lennart
authors.initials: L
authors.lastname: Marlinghaus
authors.forename: Miriam
authors.initials: M
authors.lastname: Korte
authors.forename: Sandra
authors.initials: S
authors.lastname: Neumann
authors.forename: Martin
authors.initials: M
authors.lastname: Kaase
authors.forename: Soeren G
authors.initials: SG
authors.lastname: Gatermann
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D003030:Coagulase / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000382:microbiology*; D017353:Gene Deletion; D006801:Humans; D011485:Protein Binding; D013203:Staphylococcal Infections / Q000382:microbiology; D057787:Staphylococcus lugdunensis / Q000302:isolation & purification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D003030:Coagulase; D037521:Virulence Factors