title: PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering.
abstract: By mimicking the native extracellular matrix, electrospun nanofibrous scaffolds (ENSs) can provide both chemical and physical cues to modulate cell adherence and differentiation and to promote tissue regeneration while retaining bioresorbable and biocompatible properties. In this study, ENSs were developed to deliver multiple biomolecules by loading them into the core-sheath structure and/or by conjugating them to the nanofiber surfaces. In this work, poly(L-lactide)-poly(ethylene glycol)-NH(2) and poly(L-lactide) were emulsion electrospun into nanofibers with a core-sheath structure. A model drug, tetracycline hydrochloride, was loaded within the nanofibers. Amino and carboxyl reactive groups were then activated on the fiber surfaces using saturated water vapor exposure and base hydrolysis, respectively. These reactive groups allowed the surface of the ENS to be functionalized with two other bioactive molecules, fluorescein isothiocyanate- and rhodamine-labeled bovine serum albumins, which were used as model proteins. The ENSs were shown to retain their antimicrobial capacity after two functionalization reactions, indicating that multifunctional nanofibers can potentially be developed into functional wound dressings or periodontal membranes or used in more complicated tissue systems where multiple growth factors and anti-infection precautions are critical for the successful implantation and regeneration of tissues.
authors.affiliation: Department of Mechanical Engineering, Faculty of Engineering, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Beth
authors.initials: B
authors.lastname: Zhou
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Ouyang
authors.forename: Jiming
authors.initials: J
authors.lastname: Kong
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.forename: Malcolm M Q
authors.initials: MM
authors.lastname: Xing
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D006801:Humans; D007773:Lactates / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry*; D012235:Rhodamines; D012710:Serum Albumin, Bovine; D013211:Staphylococcus aureus / Q000187:drug effects; D013752:Tetracycline / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004337:Drug Carriers; D007773:Lactates; D010455:Peptides; D012235:Rhodamines; C039217:fluorescein isothiocyanate bovine serum albumin; C088513:poly(lactic acid-ethylene glycol); D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D013752:Tetracycline; D016650:Fluorescein-5-isothiocyanate
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