title: Complete sequence of broad-host-range plasmid pRIO-5 harboring the extended-spectrum-β-lactamase gene blaBES₋₁.
abstract: Broad-host-range plasmid pRIO-5, harboring the extended-spectrum β-lactamase bla(BES-1) gene in Serratia marcescens, was fully sequenced. Analysis of the 12,957-bp sequence of this IncP6-type plasmid revealed that the bla(BES-1) gene was associated with two copies of the insertion sequence IS26. The promoter responsible for the bla(BES-1) expression was hybrid, made of a -35 box located inside the inverted repeat of IS26 and a -10 box inside a remnant of an insertion sequence.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K-Bicêtre, France.
authors.forename: Rémy A
authors.initials: RA
authors.lastname: Bonnin
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Jorge Luiz Mello
authors.initials: JL
authors.lastname: Sampaio
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D047090:beta-Lactams; C417322:beta-lactamase BES-1; D001618:beta-Lactamases
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