title: Determination of the heterogeneous interactome between Edwardsiella tarda and fish gills.
abstract: Edwardsiellosis caused by Edwardsiella tarda is a frequent occurrence throughout the world and has resulted in extensive losses in aquaculture. However, information regarding to protein-protein interaction between the pathogenic cells and host is not available although the portal of entry of the pathogen is determined. In this study, fish gill and bacterial pull-down approaches were used to isolate both bacterial outer membrane proteins that bind to gills and fish gill proteins that interact with bacterial cells, respectively. Eight interacting bacterial proteins and twelve interacting fish proteins were obtained. The genes of seven bacterial proteins were cloned and expressed for preparation of antibodies. The prepared antibodies were used to investigate protein-protein interactions between bacterial cells and fish gills. Five heterogeneous protein-protein interactions were determined. Moreover, the protective ability of three of the bacterial recombinant proteins, selected at random, was investigated in a mouse model where they showed significant protection. The gill proteins were highly homologous proteins with from humans and other animals where they are known to be involved in host immunity. These findings indicate that the heterogeneous interactome has significantly biological significance. Our results demonstrate a way to determine and understand the heterogeneous interaction between of E. tarda and gills.
authors.affiliation: Center for Proteomics, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xuanxian
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D015153:Blotting, Western; D020609:Edwardsiella tarda / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000276:immunology; D005399:Fishes / Q000276:immunology / Q000378:metabolism; D005880:Gills / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D010802:Phylogeny; D025941:Protein Interaction Mapping / Q000379:methods; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000906:Antibodies; D001425:Bacterial Outer Membrane Proteins