title: Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
abstract: BACKGROUND
  Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing.
  
  
  RESULTS
  We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples.
  
  
  CONCLUSIONS
  The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required.
authors.affiliation: Laboratorio de Parasitología, Universidad de Santiago de Compostela, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Martínez-Sernández
authors.forename: Laura
authors.initials: L
authors.lastname: Muiño
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Perteguer
authors.forename: Teresa
authors.initials: T
authors.lastname: Gárate
authors.forename: Mercedes
authors.initials: M
authors.lastname: Mezo
authors.forename: Marta
authors.initials: M
authors.lastname: González-Warleta
authors.forename: Antonio
authors.initials: A
authors.lastname: Muro
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Correia da Costa
authors.forename: Fernanda
authors.initials: F
authors.lastname: Romarís
authors.forename: Florencio M
authors.initials: FM
authors.lastname: Ubeira
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005210:Fasciola hepatica / Q000276:immunology / Q000302:isolation & purification*; D005211:Fascioliasis / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010274:Parasitology / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins
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