title: Chemopreventive effect of a mixture of Chinese Herbs (antitumor B) on chemically induced oral carcinogenesis.
abstract: In this study, we evaluated chemopreventive efficacy of Antitumor B, a Chinese herbal mixture of six plants (Sophora tonkinensis, Polygonum bistorta, Prunella vulgaris, Sonchus arvensis L., Dictamnus dasycarpus, and Dioscorea bulbifera) on the development of 4-nitroquinoline-1-oxide (4NQO) induced oral squamous cell carcinomas in A/J mice. Antitumor B, delivered through diet, inhibited 4NQO-induced oral cancer development by 59.19%. The reduction of cell proliferation appears to be associated with efficacy of Antitumor B against 4NQO-induced oral cancer in A/J mice. The expression of epidermal growth factor receptor (EGFR) and phosphorylated EGFR (Tyr1173) were down-regulated by Antitumor B. Tissue distribution of Antitumor B was determined using obacunone, matrine, and maackiain as marker chemicals. We found significant amounts of obacunone, matrine, and maackiain in the blood after 1-wk treatment. The concentrations of these three compounds did not increase further at 18  wk, suggesting that plasma concentrations had reached a steady-state level at 1  wk. There was no significant body weight loss and there was no other obvious sign of toxicity in Antitumor B-treated mice. These results suggest that Antitumor B is a promising agent for human oral cancer chemoprevention.
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: Yian
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Yao
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Weidong
authors.initials: W
authors.lastname: Wen
authors.forename: Yinqiu
authors.initials: Y
authors.lastname: Du
authors.forename: Eva
authors.initials: E
authors.lastname: Szabo
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Lubet
authors.forename: Ming
authors.initials: M
authors.lastname: You
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000470:Alkaloids / Q000097:blood; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D001584:Benzoxepins / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002273:Carcinogens / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000187:drug effects; D018890:Chemoprevention; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D066246:ErbB Receptors / Q000096:biosynthesis; D036701:Limonins / Q000097:blood; D008297:Male; D051379:Mice; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D010766:Phosphorylation; D036343:Pterocarpans / Q000097:blood; D011807:Quinolizines / Q000097:blood; D014443:Tyrosine / Q000378:metabolism; D000093842:Matrines; D000094162:Phytoalexins
chemical_list: D015112:4-Nitroquinoline-1-oxide; D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001584:Benzoxepins; D015415:Biomarkers; D002273:Carcinogens; D004365:Drugs, Chinese Herbal; D066246:ErbB Receptors; D036701:Limonins; D036343:Pterocarpans; D011807:Quinolizines; D014443:Tyrosine; D000093842:Matrines; C027128:Antitumor B; C067207:obacunone; C001449:inermin; D000094162:Phytoalexins