title: Identification of novel peptides that stimulate human neutrophils.
abstract: Neutrophils play a key role in innate immunity, and the identification of new stimuli that stimulate neutrophil activity is a very important issue. In this study, we identified three novel peptides by screening a synthetic hexapeptide combinatorial library. The identified peptides GMMWAI, MMHWAM, and MMHWFM caused an increase in intracellular Ca2+ in a concentration-dependent manner via phospholipase C activity in human neutrophils. The three peptides acted specifically on neutrophils and monocytes and not on other non-leukocytic cells. As a physiological characteristic of the peptides, we observed that the three peptides induced chemotactic migration of neutrophils as well as stimulated superoxide anion production. Studying receptor specificity, we observed that two of the peptides (GMMWAI and MMHWFM) acted on formyl peptide receptor (FPR)1 while the other peptide (MMHWAM) acted on FPR2. Since the three novel peptides were specific agonists for FPR1 or FPR2, they might be useful tools to study FPR1- or FPR2-mediated immune response and signaling.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Geon Ho
authors.initials: GH
authors.lastname: Bae
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Young Su
authors.initials: YS
authors.lastname: Jung
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Shim
authors.forename: Sang Doo
authors.initials: SD
authors.lastname: Kim
authors.forename: Suk-Hwan
authors.initials: SH
authors.lastname: Baek
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Kwon
authors.forename: Joon Seong
authors.initials: JS
authors.lastname: Park
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects*; D016716:PC12 Cells; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D044042:Receptors, Formyl Peptide / Q000819:agonists
chemical_list: D010455:Peptides; D044042:Receptors, Formyl Peptide; D002118:Calcium
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