title: Synergistic effects of bone mesenchymal stem cells and chondroitinase ABC on nerve regeneration after acellular nerve allograft in rats.
abstract: This study aimed to evaluate whether combination therapy of bone marrow stromal cells (BMSCs) transplantation and chondroitinase ABC (ChABC) treatment further enhances axonal regeneration and functional recovery after acellular nerve allograft repair of the sciatic nerve gap in rats. Eight Sprague-Dawley rats were used as nerve donors, and 32 Wistar rats were randomly divided into four groups: Group I: acellular rat sciatic nerve (ARSN) group; Group II: ChABC treatment; Group III: BMSCs transplantation; and Group IV: ChABC treatment and BMSCs transplantation. The results showed that compared with ARSN control group, BMSC transplantation promoted axonal regeneration, the secretion of neural trophic factors NGF, BDNF and axon angiogenesis in nerve graft. ChABC treatment degraded chondroitin sulfate proteoglycans in ARSN in vitro and in vivo and improved BMSCs survival in ARSN. The combination therapy caused much better beneficial effects evidenced by increasing sciatic function index, nerve conduction velocity, restoration rate of tibialis anterior wet muscle weight, and myelinated nerve number, but did not further boost the therapeutic effects on neurotrophic factor production, axon angiogenesis, and sensory functional recovery by BMSC transplantation. Taken together, for the first time, we demonstrate the synergistic effects of BMSC transplantation and BMSCs treatment on peripheral nerve regeneration, and our findings may help establish novel strategies for cell transplantation therapy for peripheral nerve injury.
authors.affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Jia
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Li
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Tong
authors.forename: Gui-Bo
authors.initials: GB
authors.lastname: Liu
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D019765:Chondroitin ABC Lyase / Q000008:administration & dosage*; D005260:Female; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D009417:Nerve Tissue / Q000201:enzymology / Q000637:transplantation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000378:metabolism / Q000601:surgery*; D014184:Transplantation, Homologous / Q000379:methods
chemical_list: D019765:Chondroitin ABC Lyase
references.citation: Eplasty. 2010 Jun 14;10:e42
references.pmid: 20563304
references.citation: Muscle Nerve. 2011 Feb;43(2):157-63
references.pmid: 21254077
references.citation: J Neurosurg. 2004 Nov;101(5):806-12
references.pmid: 15540919
references.citation: Clin Plast Surg. 1984 Jan;11(1):3-8
references.pmid: 6705472
references.citation: FASEB J. 2004 Jan;18(1):194-6
references.pmid: 14630702
references.citation: Exp Neurol. 2007 Jan;203(1):185-95
references.pmid: 16970940
references.citation: Restor Neurol Neurosci. 2008;26(2-3):131-45
references.pmid: 18820407
references.citation: J Clin Neurosci. 2008 Feb;15(2):185-91
references.pmid: 18078754
references.citation: Ann Plast Surg. 2011 Aug;67(2):147-55
references.pmid: 21712696
references.citation: Exp Neurol. 2007 Mar;204(1):443-53
references.pmid: 17222827
references.citation: Cell Mol Neurobiol. 2009 May;29(3):347-53
references.pmid: 18987968
references.citation: Biotechnol Bioeng. 2010 Aug 15;106(6):970-9
references.pmid: 20589674
references.citation: Neurol Res. 2002 Oct;24(7):634-8
references.pmid: 12392196
references.citation: Biotechnol Appl Biochem. 2004 Aug;40(Pt 1):17-24
references.pmid: 15270703
references.citation: J Neurosci. 2001 Aug 15;21(16):6206-13
references.pmid: 11487643
references.citation: J Neurosci. 2005 Feb 2;25(5):1169-78
references.pmid: 15689553
references.citation: Neurol Res. 2004 Mar;26(2):230-2
references.pmid: 15072644
references.citation: Nat Rev Neurosci. 2004 Feb;5(2):146-56
references.pmid: 14735117
references.citation: Nan Fang Yi Ke Da Xue Xue Bao. 2010 Sep;30(9):2030-5
references.pmid: 20855243
references.citation: Spine (Phila Pa 1976). 1996 Sep 1;21(17):1952-6
references.pmid: 8883193
references.citation: Am J Orthop (Belle Mead NJ). 2000 Mar;29(3):167-73
references.pmid: 10746467
references.citation: Exp Neurol. 2007 Sep;207(1):163-70
references.pmid: 17669401
references.citation: Muscle Nerve. 1978 Jan-Feb;1(1):70-4
references.pmid: 88010