title: Establishment of a Chinook salmon cell line with an inducible gene expression system.
abstract: We have isolated a stable recombinant cell line CHSE-TOF5 derived from the Chinook salmon (Oncorhynchus tshawytscha) embryo cells for use as an inducible expression system. The cells were transfected with the pTet-Off plasmid from the Tet On/Off Clontech system, carrying a G418 resistance gene. Several G418-resistant clones were subcultured and characterised by quantitative PCR (qPCR) and by transient transfection. The level of expression of transcriptional activator was measured by qPCR in a number of isolated clones, and transient transfection with a pTRE2-hyg-LUC plasmid was used to evaluate the inducibility of these clones. A clone was selected for its relative fast cell growth and good level of inducibility. This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of proteins from fish or fish pathogens.
authors.affiliation: Immunology and Infection, Marine Scotland, Marine Laboratory, PO Box 101, 375 Victoria Road, Aberdeen, AB11 9DB, UK. Bertrand.Collet@scotland.gsi.gov.uk
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
authors.forename: Katherine
authors.initials: K
authors.lastname: Lester
mesh_terms: D000818:Animals; D002460:Cell Line / Q000166:cytology* / Q000378:metabolism*; D002999:Clone Cells; D015870:Gene Expression / Q000187:drug effects; D005821:Genetic Techniques; D008156:Luciferases / Q000378:metabolism; D012474:Salmon; D013752:Tetracycline / Q000494:pharmacology; D015534:Trans-Activators / Q000378:metabolism; D014162:Transfection
chemical_list: D015534:Trans-Activators; D008156:Luciferases; D013752:Tetracycline
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