title: Calcitonin gene-related peptide suppresses  isoprenaline-induced cardiomyocyte apoptosis  through regulation of microRNA-1 and  microRNA-133a expression.
abstract: OBJECTIVE
  To explore the inhibitory effect of calcitonin gene-related peptide (CGRP) on cardiomyocyte apoptosis and the underlying mechanism.
  
  
  METHODS
  In cultured rat cardiomyocytes, apoptosis was induced by the incubation of isoprenaline (10(-5) mol/L) for 48 h. CGRP (10(-8) or 10(-7) mol/L) was administrated for 1 h before incubating isoprenaline to evaluate its effect on cardiomyocyte apoptosis. At the end of the drug treatment, the rate of apoptotic cells and intracellular reactive oxygen species (ROS) were determined, and RNA was extracted to examine the expression of microRNA-1 and microRNA-133a.
  
  
  RESULTS
  Isoprenaline significantly increased the rate of apoptotic cells and intracellular ROS production concomitantly with the increased microRNA-1 expression and the decreased microRNA-133a expression, which were inhibited by pretreatment with CGRP. The effects of CGRP were reversed by CGRP receptor antagonist.
  
  
  CONCLUSIONS
  CGRP can inhibit the isoprenaline-induced cardiomyocyte apoptosis. The beneficial effect of CGRP is related to regulating microRNA-1 and microRNA-133a expression through the prevention of isoprenaline-induced ROS production.
authors.affiliation: Department of Pharmacology, Central South University, Changsha 410078, China.
authors.forename: Jianzhe
authors.initials: J
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.forename: Chenjing
authors.initials: C
authors.lastname: Wang
authors.forename: Hanwu
authors.initials: H
authors.lastname: Deng
authors.forename: Yuanjian
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D007545:Isoproterenol / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C542191:MIRN1 microRNA, rat; C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D017382:Reactive Oxygen Species; D015740:Calcitonin Gene-Related Peptide; D007545:Isoproterenol