title: Development and clinical evaluation of a rapid serodiagnostic test for toxoplasmosis of cats using recombinant SAG1 antigen.
abstract: Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.
authors.affiliation: Department of Biochemistry, College of Natural Science, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.
authors.forename: Chom-Kyu
authors.initials: CK
authors.lastname: Chong
authors.forename: Wooseog
authors.initials: W
authors.lastname: Jeong
authors.forename: Hak-Yong
authors.initials: HY
authors.lastname: Kim
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: An
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Jeoung
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Ryu
authors.forename: A-Ra
authors.initials: AR
authors.lastname: Ko
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Sung-Jong
authors.initials: SJ
authors.lastname: Hong
authors.forename: Zhaoshou
authors.initials: Z
authors.lastname: Yang
authors.forename: Ho-Woo
authors.initials: HW
authors.lastname: Nam
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000235:genetics; D019095:Point-of-Care Systems; D015800:Protozoan Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014122:Toxoplasma / Q000235:genetics; D014124:Toxoplasmosis, Animal / Q000175:diagnosis*; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C047168:SAG1 antigen, Toxoplasma
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