title: High frequency of glucose-utilizing mutants in Shewanella oneidensis MR-1.
abstract: Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis, which cannot immediately use glucose as a sole carbon substrate. High-performance liquid chromatography and (14)C glucose tracer studies confirm the disappearance in cultures and assimilation and respiration, respectively, of glucose. The relatively short time frame with which S. oneidensis MR-1 gained the ability to use glucose raises interesting ecological implications.
authors.affiliation: National Research Council, Washington, DC, USA.
authors.forename: Erinn C
authors.initials: EC
authors.lastname: Howard
authors.forename: Leila J
authors.initials: LJ
authors.lastname: Hamdan
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Lizewski
authors.forename: Bradley R
authors.initials: BR
authors.lastname: Ringeisen
mesh_terms: D005947:Glucose / Q000378:metabolism*; D009154:Mutation; D020592:Shewanella / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005947:Glucose