title: Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene.
abstract: An Entamoeba histolytica strain (BF-841 cl1) that originated from Burkina Faso, Africa presented with novel, polymorphic genotypes of the serine-rich E. histolytica protein and the anodic hexokinase‑2 (HXK-2) isoenzyme band, which showed less electrophoretic mobility than that of an E. histolytica reference strain [HM-1:IMSS cl6 (zymodeme (Z)-II)] by starch gel electrophoresis and isoelectric focusing (IEF). The HXK-2 gene of BF-841 cl1 had amino acid variations at four positions compared to the sequence of HM-1:IMSS cl6. These variations were absent from the sequences of four other E. histolytica strains with different zymodemes [KU27 (Z-II), SAW1627 (Z-IIa-), SAW755CR clB (Z-XIV), and KU2 (Z-XIX)]. The results of IEF showed no difference in the substrate specificity of HXK (HXK-1 and HXK-2) between BF-841 cl1 and the three reference E. histolytica strains (HM-1:IMSS cl6, SAW755 clB, and KU27). It was also confirmed that BF-841 cl1 was able to form liver abscesses in Syrian hamsters.
authors.affiliation: Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Imada
authors.forename: M E
authors.initials: ME
authors.lastname: Tolba
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002050:Burkina Faso; D006224:Cricetinae; D004592:Electrophoresis, Starch Gel; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D005838:Genotype; D006593:Hexokinase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008101:Liver Abscess, Amebic / Q000469:parasitology*; D008647:Mesocricetus; D011110:Polymorphism, Genetic; D017385:Sequence Homology; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D006593:Hexokinase
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