title: Upregulated miR-29b promotes neuronal cell death by inhibiting Bcl2L2 after ischemic brain injury.
abstract: It is increasingly clear that microRNAs (miRNAs) play an important role in controlling cell survival. However, the functional significance of miRNAs in ischemic brain injury remains poorly understood. In the present study, we assayed the expression levels of miR-29b after ischemic brain injury, and defined the target genes and biological functions of miR-29b. We found that the miR-29b levels were significantly increased in rat brain after transient middle cerebral artery occlusion and neurons after oxygen-glucose deprivation. Moreover, ectopic expression of miR-29b promoted neuronal cell death, whereas its repression decreased cell death. Furthermore, we verified that miR-29b directly targeted and inhibited Bcl2L2 gene expression, and then increased neuronal cell death. Importantly, Bcl2L2 overexpression rescued neuronal cell death induced by miR-29b. These results suggest an important role of miR-29b in regulating neuronal cell death, thus offering a new target for the development of therapeutic agents against ischemic brain injury.
authors.affiliation: Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, People's Republic of China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Tielong
authors.initials: T
authors.lastname: Liu
authors.forename: Wangjun
authors.initials: W
authors.lastname: Yan
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiangang
authors.initials: J
authors.lastname: Shi
authors.forename: Lianshun
authors.initials: L
authors.lastname: Jia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001930:Brain Injuries / Q000235:genetics* / Q000473:pathology*; D002545:Brain Ischemia / Q000235:genetics* / Q000473:pathology*; D016923:Cell Death / Q000235:genetics*; D002478:Cells, Cultured; D005947:Glucose / Q000172:deficiency; D002534:Hypoxia, Brain / Q000473:pathology; D007202:Indicators and Reagents; D020244:Infarction, Middle Cerebral Artery / Q000235:genetics / Q000473:pathology; D008156:Luciferases / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020521:Stroke / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation / Q000502:physiology
chemical_list: C491072:Bcl2l2 protein, rat; D007202:Indicators and Reagents; C526366:MIRN29 microRNA, rat; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D008156:Luciferases; D005947:Glucose
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