title: The role of endogenous dynorphin in ethanol-induced state-dependent CPP.
abstract: The aim of this study was to determine the role of the endogenous dynorphin/kappa opioid receptor (DYN/KOP) system in ethanol-induced state-dependent conditioned place preference (CPP). To this end, mice lacking the pro-DYN gene and their wild-type littermates/controls were tested for baseline place preference on day 1, received 15-min morning and afternoon conditionings with saline or ethanol (2g/kg) each day for three consecutive days and were then tested for CPP under a drug-free state on day 5 and following a saline or ethanol (1 or 2g/kg) challenge on day 8. Given that compensatory developmental changes may occur in knockout mice, the effect of nor-binaltorphimine (nor-BNI), a KOP antagonist, on state-dependent CPP induced by ethanol was also studied in wild-type mice. On day 1, mice were tested for baseline place preference and, 4h later, treated with saline or nor-BNI (10mg/kg). On days 2-4, mice received 15-min morning and afternoon conditionings and were tested for CPP under a drug-free state on day 5 and following an ethanol (1g/kg) challenge on day 8. A comparable CPP was observed in mice lacking the pro-DYN gene and their wild-type littermates/controls as well as in wild-type mice treated with nor-BNI and their saline-treated controls. However, these mice compared to their respective controls exhibited a greater CPP response following an ethanol (1g/kg) challenge, suggesting that the endogenous DYN/KOP system may negatively regulate ethanol-induced state-dependent CPP.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, CA 91766, United States.
authors.forename: Khanh
authors.initials: K
authors.lastname: Nguyen
authors.forename: Andy
authors.initials: A
authors.lastname: Tseng
authors.forename: Paul
authors.initials: P
authors.lastname: Marquez
authors.forename: Abdul
authors.initials: A
authors.lastname: Hamid
authors.forename: Kabirullah
authors.initials: K
authors.lastname: Lutfy
mesh_terms: D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D003216:Conditioning, Operant / Q000187:drug effects*; D004334:Drug Administration Schedule; D004399:Dynorphins / Q000378:metabolism*; D004745:Enkephalins / Q000172:deficiency; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D011498:Protein Precursors / Q000172:deficiency; D012737:Sex Factors; D013997:Time Factors
chemical_list: D002492:Central Nervous System Depressants; D004745:Enkephalins; D009292:Narcotic Antagonists; D011498:Protein Precursors; C051844:norbinaltorphimine; D000431:Ethanol; D009271:Naltrexone; D004399:Dynorphins; C033660:preproenkephalin
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