title: Technology trends in antibody purification.
abstract: This article reviews technology trends in antibody purification. Section 1 discusses non-chromatography methods, including precipitation, liquid-liquid extraction, and high performance tangential flow filtration. The second addresses chromatography methods. It begins with discussion of fluidized and fixed bed formats. It continues with stationary phase architecture: diffusive particles, perfusive particles, membranes and monoliths. The remainder of the section reviews recent innovations in size exclusion, anion exchange, cation exchange, hydrophobic interaction, immobilized metal affinity, mixed-mode, and bioaffinity chromatography. Section 3 addresses an emerging trend of formulating process buffers to prevent or correct anomalies in the antibodies being purified. Methods are discussed for preventing aggregate formation, dissociating antibody-contaminant complexes, restoring native antibody from aggregates, and conserving or restoring native disulfide pairing.
authors.affiliation: Bioprocessing Technology Institute, 20 Biopolis Way, 06-01 Centros, Singapore 138668. pete gagnon@bti.a-star.edu.sg
authors.forename: Pete
authors.initials: P
authors.lastname: Gagnon
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D005374:Filtration; D006801:Humans; D059625:Liquid-Liquid Extraction
chemical_list: D000906:Antibodies