title: Synthesis of 99mTc-nimotuzumab with tricarbonyl ion: in vitro and in vivo studies.
abstract: The Epidermal growth factor receptor (EGFR) family plays an important role in carcinogenesis. CIMAher® (Nimotuzumab), is a humanized monoclonal antibody, which recognizes EGFR with high affinity. The aim of this work was to perform the direct labeling of Nimotuzumab with [99mTc(CO)3(H2O)3]+ as precursor and to evaluate its labeling conditions, in vitro and in vivo stability and biodistrution in normal C57 BL/6J mice. 99mTc(CO3)-Nimotuzumab labeling yields were up to 90%. More than 90% of the complex remained intact after 24 h of incubation with L-Histidine (1/300 molar ratio). Biodistribution studies in normal mice were also performed. Inmunoreactivity was confirmed by cell binding assays with A431cells. These results encourage the evaluation of the potential role of 99mTc(CO)3-Nimotuzumab as a novel tumor-avid radiotracer for targeting in vivo EGFR expression.
authors.affiliation: Laboratorio de Radiofarmacia, CIN, Montevideo, Uruguay.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Garcia
authors.forename: Ximena
authors.initials: X
authors.lastname: Camacho
authors.forename: Victoria
authors.initials: V
authors.lastname: Calzada
authors.forename: Marcelo
authors.initials: M
authors.lastname: Fernandez
authors.forename: Williams
authors.initials: W
authors.lastname: Porcal
authors.forename: Omar
authors.initials: O
authors.lastname: Alonso
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Gambini
authors.forename: Pablo
authors.initials: P
authors.lastname: Cabral
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000378:metabolism* / Q000493:pharmacokinetics; D066246:ErbB Receptors / Q000378:metabolism*; D005240:Feasibility Studies; D007553:Isotope Labeling; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D015609:Organotechnetium Compounds / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C501466:nimotuzumab; D066246:ErbB Receptors