title: The relationship of leukocyte anisocytosis to holotranscobalamin, a marker of cobalamin deficiency.
abstract: BACKGROUND
  After measurement of the mean volumes of leukocyte subpopulations as well as the distribution widths (DW) of these volumes has become available, we investigated whether such morphometric leukocyte parameters are associated with a commonly used marker of cobalamin deficiency, i.e., holotranscobalamin (HoloTC). Further, we determined reference intervals for these parameters in an elderly population.
  
  
  METHODS
  Consecutive subjectively healthy and volunteering individuals ≥60 years were included. Using the UniCel DxH 800 Coulter Cellular Analysis System MoMV, mean neutrophil volume (NeMV), mean lymphocyte volume (LyMV), monocyte anisocytosis (MoV-DW), neutrophil anisocytosis (NeV-DW), and lymphocyte anisocytosis (LyV-DW) were assessed together with other parameters including HoloTC.
  
  
  RESULTS
  A total of 150 individuals were included in the study. Reference intervals were not dependent on age and gender. MoV-DW (P = 0.002) and NeV-DW (P = 0.02) were significantly lower, and LyMV was significantly higher (P = 0.04) in participants with a HoloTC concentration <28 pm. In contrast, MCV, MoMV, NeMV, and LyV-DW were not associated with HoloTC concentrations. The area under the curve (AUC) in the receiver operating characteristic analysis for detecting a HoloTC <28 pm was 0.81 [95% confidence interval (CI) (0.73, 0.87)] for MoV-DW and 0.73 (0.66, 0.80) for NeV-DW.
  
  
  CONCLUSIONS
  In this collective of subjectively healthy elderly individuals, monocyte anisocytosis, neutrophil anisocytosis and mean lymphocyte volume were associated with decreased HoloTC.
authors.affiliation: Private University Liechtenstein, Triesen, Liechtenstein. lorenzrisch@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Risch
authors.forename: P
authors.initials: P
authors.lastname: Medina
authors.forename: U E
authors.initials: UE
authors.lastname: Nydegger
authors.forename: Z
authors.initials: Z
authors.lastname: Bahador
authors.forename: T
authors.initials: T
authors.lastname: Brinkmann
authors.forename: P
authors.initials: P
authors.lastname: Von Landenberg
authors.forename: M
authors.initials: M
authors.lastname: Risch
authors.forename: L
authors.initials: L
authors.lastname: Risch
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019540:Area Under Curve; D048429:Cell Size; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012015:Reference Standards; D014155:Transcobalamins / Q000172:deficiency*; D014806:Vitamin B 12 Deficiency / Q000097:blood*
chemical_list: D014155:Transcobalamins