title: Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea.
abstract: Genetic diversity and antiviral activity for five common antiviral drugs of echovirus (ECV) 5 isolated in Korea have been described. The present study extended these tests to a Korean ECV 18 isolate. An outbreak of aseptic meningitis caused by the ECV 18 isolate was reported in Korea in 2005, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from stool specimen of a 5-year-old male patient with aseptic meningitis, the complete genome sequence was obtained and was compared it with the Metcalf prototype strain. Unlike the ECV5 isolate, the 3' untranslated region had the highest identity value (94.2%) at the nucleotide level, while, at the amino acid level, the P2 region displayed the highest identity value (96.9%). These two strains shared all cleavage sites, with the exception of the 2B/2C site, which was RQ/NN in the Metcalf strain but RQ/NS in the Korean ECV 18 isolate. In Vero cells infected with the Korean ECV 18 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 4.97 ± 0.77 μg/mL, TI: 20.12) and ribavirin (IC50: 7.63 ± 0.87 μg/mL, TI: 13.11) had any antiviral activity against the Korean ECV 18 isolate in the five antiviral drugs. These antiviral activity effects were similar with results of the Korean ECV5 isolate.
authors.affiliation: Department of Microbiology, Chungcheongnam-Do Institute of Health and Environmental Research, Daejeon, Korea.
authors.forename: Kwisung
authors.initials: K
authors.lastname: Park
authors.forename: SangGu
authors.initials: S
authors.lastname: Yeo
authors.forename: KyoungAh
authors.initials: K
authors.lastname: Baek
authors.forename: DooSung
authors.initials: D
authors.lastname: Cheon
authors.forename: YoungJin
authors.initials: Y
authors.lastname: Choi
authors.forename: JoonSoo
authors.initials: J
authors.lastname: Park
authors.forename: SooJin
authors.initials: S
authors.lastname: Lee
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004196:Disease Outbreaks; D004457:Echovirus Infections / Q000453:epidemiology / Q000821:virology; D029822:Enterovirus B, Human / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D020128:Inhibitory Concentration 50; D007723:Korea / Q000453:epidemiology; D008297:Male; D008582:Meningitis, Aseptic / Q000453:epidemiology / Q000821:virology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D012367:RNA, Viral
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