title: Changes in cell migration of mesenchymal cells during osteogenic differentiation.
abstract: We showed that the migration, morphology and adhesiveness of undifferentiated mesenchymal cells dramatically changed during osteogenic differentiation. The migration of these cells was transiently upregulated early in osteogenic differentiation. At a later stage, migration was decreased but adhesiveness was increased. Furthermore, Cdc42 and Rac1 Rho-family small GTPases were activated at early stages of differentiation and the phosphorylation level of FAK decreased as differentiation progressed. We also showed cell migration was promoted by inhibition of the Rho-ROCK-myosin signaling. Finally, using a mouse model of ectopic bone formation, we confirmed that treatment with ROCK inhibitor, Y-27632 increased cell movement into bone formation sites, resulting in enhanced osteogenesis. These results provide a new insight into the link between cell migration and osteogenic differentiation.
authors.affiliation: Department of Biology, Osaka Medical Center of Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Ichida
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yui
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Toru
authors.initials: T
authors.lastname: Wakamatsu
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Itoh
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D017102:Fracture Healing / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D047428:Protein Kinase Inhibitors; D054460:rho-Associated Kinases