title: Hybrid carbon-based scaffolds for applications in soft tissue reconstruction.
abstract: Current biomedical scaffolds utilized in surgery to repair soft tissues commonly fail to meet the optimal combination of biomechanical and tissue regenerative properties. Carbon is a scaffold alternative that potentially optimizes the balance between mechanical strength, durability, and function as a cell and biologics delivery vehicle that is necessary to restore tissue function while promoting tissue repair. The goals of this study were to investigate the feasibility of fabricating hybrid fibrous carbon scaffolds modified with biopolymer, polycaprolactone and to analyze their mechanical properties and ability to support cell growth and proliferation. Environmental scanning electron microscopy, micro-computed tomography, and cell adhesion and cell proliferation studies were utilized to test scaffold suitability as a cell delivery vehicle. Mechanical properties were tested to examine load failure and elastic modulus. Results were compared to an acellular dermal matrix scaffold control (GraftJacket(®) [GJ] Matrix), selected for its common use in surgery for the repair of soft tissues. Results indicated that carbon scaffolds exhibited similar mechanical maximums and capacity to support fibroblast adhesion and proliferation in comparison with GJ. Fibroblast adhesion and proliferation was collinear with carbon fiber orientation in regions of sparsely distributed fibers and occurred in clusters in regions of higher fiber density and low porosity. Overall, fibroblast adhesion and proliferation was greatest in lower porosity carbon scaffolds with highly aligned fibers. Stepwise multivariate regression showed that the variability in maximum load of carbon scaffolds and controls were dependent on unique and separate sets of parameters. These finding suggested that there were significant differences in the functional implications of scaffold design and material properties between carbon and dermis derived scaffolds that affect scaffold utility as a tissue replacement construct.
authors.affiliation: Carbon Research Laboratory, University of Dayton Research Institute, Dayton, Ohio 45469, USA. jczarnecki1@notes.udayton.edu
authors.forename: Jarema S
authors.initials: JS
authors.lastname: Czarnecki
authors.forename: Khalid
authors.initials: K
authors.lastname: Lafdi
authors.forename: Robert M
authors.initials: RM
authors.lastname: Joseph
authors.forename: Panagiotis A
authors.initials: PA
authors.lastname: Tsonis
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008855:Microscopy, Electron, Scanning; D017695:Soft Tissue Injuries / Q000628:therapy*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002244:Carbon
references.citation: Clin Orthop Relat Res. 1991 Oct;(271):225-32
references.pmid: 1914301
references.citation: Acta Orthop Belg. 2011 Jun;77(3):281-9
references.pmid: 21845993
references.citation: Biomaterials. 1996 Jun;17(11):1121-6
references.pmid: 8718973
references.citation: Clin Sports Med. 2009 Apr;28(2):191-201, vii
references.pmid: 19306729
references.citation: J Biomed Mater Res. 2001 Jul;56(1):101-8
references.pmid: 11309796
references.citation: Arthroscopy. 2009 Mar;25(3):329-33
references.pmid: 19245998
references.citation: Br J Plast Surg. 2003 Dec;56(8):775-83
references.pmid: 14615252
references.citation: J Biomater Sci Polym Ed. 1999;10(7):715-28
references.pmid: 10426228
references.citation: J Biomed Mater Res B Appl Biomater. 2009 Aug;90(2):629-34
references.pmid: 19213050
references.citation: J Foot Ankle Surg. 2011 Mar-Apr;50(2):257-65
references.pmid: 21354014
references.citation: Tissue Eng Part A. 2010 Dec;16(12):3669-77
references.pmid: 20673132
references.citation: Clin Podiatr Med Surg. 2009 Oct;26(4):507-23
references.pmid: 19778685
references.citation: Clin Orthop Relat Res. 1986 Jan;(202):173-83
references.pmid: 3955946
references.citation: J Burn Care Rehabil. 1997 Nov-Dec;18(6):496-9
references.pmid: 9404982
references.citation: Clin Biomech (Bristol, Avon). 2000 Jul;15(6):456-62
references.pmid: 10771125
references.citation: Int Wound J. 2005 Jun;2(2):161-5
references.pmid: 16722865
references.citation: J Am Podiatr Med Assoc. 2009 Mar-Apr;99(2):104-7
references.pmid: 19299345
references.citation: Foot Ankle Int. 2008 Mar;29(3):329-33
references.pmid: 18348831
references.citation: Biomaterials. 1989 Sep;10(7):435-40
references.pmid: 2804233
references.citation: J Foot Ankle Surg. 2007 Nov-Dec;46(6):451-5
references.pmid: 17980842
references.citation: Arch Intern Med. 1994 Jan 24;154(2):164-8
references.pmid: 8285811
references.citation: Wound Repair Regen. 1999 Jul-Aug;7(4):201-7
references.pmid: 10781211
references.citation: J Bone Joint Surg Am. 2006 Dec;88(12):2673-86
references.pmid: 17142418
references.citation: J Nanosci Nanotechnol. 2007 Mar;7(3):814-7
references.pmid: 17450838
references.citation: Biomaterials. 2005 May;26(13):1523-32
references.pmid: 15522754
references.citation: J Shoulder Elbow Surg. 2010 Mar;19(2 Suppl):104-9
references.pmid: 20188275
references.citation: Biomaterials. 2010 Jan;31(3):428-37
references.pmid: 19828192
references.citation: Artif Organs. 2010 Dec;34(12):1158-63
references.pmid: 20545668
references.citation: J Surg Res. 1998 Dec;80(2):300-3
references.pmid: 9878328
references.citation: Int Orthop. 2007 Dec;31(6):729-34
references.pmid: 17639384
references.citation: Biomaterials. 2011 Apr;32(10):2479-88
references.pmid: 21196047
references.citation: Am J Sports Med. 2011 Mar;39(3):649-55
references.pmid: 21062938
references.citation: J Biomech. 2011 Feb 24;44(4):694-9
references.pmid: 21111422
references.citation: J Bone Joint Surg Am. 2009 May;91(5):1159-71
references.pmid: 19411465
references.citation: J Orthop Surg Res. 2008 Jul 24;3:32
references.pmid: 18652675
references.citation: Biomaterials. 2009 Nov;30(32):6469-77
references.pmid: 19712968
references.citation: Expert Rev Med Devices. 2009 Sep;6(5):499-505
references.pmid: 19751122
references.citation: J Foot Ankle Surg. 2009 Mar-Apr;48(2):163-9
references.pmid: 19232968
references.citation: J Mater Sci Mater Med. 2010 Sep;21(9):2611-22
references.pmid: 20532961
references.citation: J Biomed Mater Res A. 2004 Jul 1;70(1):129-38
references.pmid: 15174117
references.citation: Curr Opin Chem Biol. 2005 Dec;9(6):674-9
references.pmid: 16233988
references.citation: J Foot Ankle Surg. 2008 Jan-Feb;47(1):8-12
references.pmid: 18156058
references.citation: Tissue Eng Part A. 2008 Feb;14(2):255-65
references.pmid: 18333778