title: Effects of proteasome inhibitors on the nucleolar size of porcine oocytes.
abstract: During the final stage of oocyte growth, the morphology of the oocyte nucleoli changes into a compact structure. The objective of this study was to determine the involvement of the proteasome, which is a large protein complex responsible for degrading intracellular proteins, in the nucleolar compaction. The mean nucleolar diameter of growing porcine oocytes (about 100 µm in diameter) was larger than that of fully grown (120 µm) oocytes (15.5 ± 0.3 vs. 13.2 ± 0.1 µm, P<0.05). When fully grown oocytes were treated with proteasome inhibitors, MG132 (10 and 20 µM) and lactacystin (100 and 200 µM), the nucleolar diameter significantly increased from 12.9 µm to 14.9-16.1 µm. In contrast, transcription inhibitors, actinomycin D (0.8-8 µM) and α-amanitin (10-100 µM) reduced the nucleolar diameter of growing oocytes to 9.4-12.4 µm. MG132 partially prevented this reduction in nucleolar diameter. These results suggest that the proteasome regulates the nucleolar size in porcine oocytes perhaps through the degradation of nucleolar proteins.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Jitsukawa
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Kyogoku
authors.forename: Sugako
authors.initials: S
authors.lastname: Ogushi
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyano
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D053959:Alpha-Amanitin / Q000494:pharmacology; D000818:Animals; D002466:Cell Nucleolus / Q000187:drug effects* / Q000502:physiology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D007976:Leupeptins / Q000494:pharmacology; D009687:Nuclear Proteins / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects* / Q000648:ultrastructure*; D061988:Proteasome Inhibitors; D013552:Swine / Q000254:growth & development*
chemical_list: D053959:Alpha-Amanitin; D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D009687:Nuclear Proteins; D061988:Proteasome Inhibitors; C067713:lactacystin; D003609:Dactinomycin; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde; D000111:Acetylcysteine