title: Membrane-bound transporter controls the circadian transcription of clock genes in Drosophila.
abstract: Little is known about molecular mechanisms that control the Drosophila circadian clock beyond the transcriptional-translational feedback regulation of clock genes as an intracellular process. In this study, Early gene at 23 (E23) was identified as a novel clock gene that encodes the membrane-bound ABC transporter that is induced by the molting hormone ecdysone. E23 expresses in pacemaker neurons in fly head, and its knockdown flies lengthened circadian period with an increased expression of the clock gene vrille. E23 and vrille responded to both ecdysone and clock signals, whereas E23 protein specifically suppressed the ecdysone response and is necessary for rhythmicity. Thus, E23 forms its own feedback loop in the ecdysone response to control circadian oscillation through ecdysone-mediated vrille expression. The ecdysone signaling pathway with E23 is essential not only in developmental stage but also for the circadian behavior in adult fly.
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, Hakozaki, Fukuoka, Japan.
authors.forename: Taichi Q
authors.initials: TQ
authors.lastname: Itoh
authors.forename: Teiichi
authors.initials: T
authors.lastname: Tanimura
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001683:Biological Clocks / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000187:drug effects / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004440:Ecdysone / Q000378:metabolism / Q000494:pharmacology*; D025461:Feedback, Physiological / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D008156:Luciferases / Q000032:analysis; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; C413187:E23 protein, Drosophila; D014157:Transcription Factors; C108498:vri protein, Drosophila; D004440:Ecdysone; D008156:Luciferases