title: Requirement of argininosuccinate lyase for systemic nitric oxide production.
abstract: Nitric oxide (NO) is crucial in diverse physiological and pathological processes. We show that a hypomorphic mouse model of argininosuccinate lyase (encoded by Asl) deficiency has a distinct phenotype of multiorgan dysfunction and NO deficiency. Loss of Asl in both humans and mice leads to reduced NO synthesis, owing to both decreased endogenous arginine synthesis and an impaired ability to use extracellular arginine for NO production. Administration of nitrite, which can be converted into NO in vivo, rescued the manifestations of NO deficiency in hypomorphic Asl mice, and a nitric oxide synthase (NOS)-independent NO donor restored NO-dependent vascular reactivity in humans with ASL deficiency. Mechanistic studies showed that ASL has a structural function in addition to its catalytic activity, by which it contributes to the formation of a multiprotein complex required for NO production. Our data demonstrate a previously unappreciated role for ASL in NOS function and NO homeostasis. Hence, ASL may serve as a target for manipulating NO production in experimental models, as well as for the treatment of NO-related diseases.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Ayelet
authors.initials: A
authors.lastname: Erez
authors.forename: Sandesh C S
authors.initials: SC
authors.lastname: Nagamani
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Shchelochkov
authors.forename: Muralidhar H
authors.initials: MH
authors.lastname: Premkumar
authors.forename: Philippe M
authors.initials: PM
authors.lastname: Campeau
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Chen
authors.forename: Harsha K
authors.initials: HK
authors.lastname: Garg
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Asad
authors.initials: A
authors.lastname: Mian
authors.forename: Terry K
authors.initials: TK
authors.lastname: Bertin
authors.forename: Jennifer O
authors.initials: JO
authors.lastname: Black
authors.forename: Heng
authors.initials: H
authors.lastname: Zeng
authors.forename: Yaoping
authors.initials: Y
authors.lastname: Tang
authors.forename: Anilkumar K
authors.initials: AK
authors.lastname: Reddy
authors.forename: Marshall
authors.initials: M
authors.lastname: Summar
authors.forename: William E
authors.initials: WE
authors.lastname: O'Brien
authors.forename: David G
authors.initials: DG
authors.lastname: Harrison
authors.forename: William E
authors.initials: WE
authors.lastname: Mitch
authors.forename: Juan C
authors.initials: JC
authors.lastname: Marini
authors.forename: Judy L
authors.initials: JL
authors.lastname: Aschner
authors.forename: Nathan S
authors.initials: NS
authors.lastname: Bryan
authors.forename: Brendan
authors.initials: B
authors.lastname: Lee
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001123:Argininosuccinate Lyase / Q000378:metabolism*; D001124:Argininosuccinate Synthase / Q000378:metabolism; D056807:Argininosuccinic Aciduria / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004195:Disease Models, Animal; D042783:Endothelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000172:deficiency*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D009573:Nitrites; D034741:RNA, Small Interfering; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D001123:Argininosuccinate Lyase; D001124:Argininosuccinate Synthase
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