title: Clobetasol synergistically diminishes Ciz1 expression with genistein             in U937 cells.
abstract: Cip-interacting zinc finger protein 1 (Ciz1) stimulates DNA replication             and has been implicated in the tumorigenesis of breast cancer cells. In order             to investigate the possibility of using medicinal glucocorticoids against breast             cancer, we studied whether certain glucocorticoids affect the expression of Ciz1.             The in vitro effect of clobetasol treatment on the reduction of Ciz1 expression             was detected by reverse transcriptase-polymerase chain reaction. Western blotting             also confirmed the down-regulation of the protein in a dose-dependent manner upon             clobetasol treatment in U937 monocytoid cells. Furthermore, we found that Ciz1             protein expression was decreased after pre-treatment of the cells with clobetasol             and genistein. An extract of Lens culinaris also had a synergistic effect on the             repression of Ciz1 protein expression.
authors.affiliation: Department of Environmental Health Science, Nara Women's University,                     Nara 630-8506, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Okumura
authors.forename: Hitomi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kitagishi
authors.forename: Satoru
authors.initials: S
authors.lastname: Matsuda
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002990:Clobetasol / Q000494:pharmacology*; D004261:DNA Replication; D015536:Down-Regulation / Q000187:drug effects*; D004357:Drug Synergism; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D027806:Lens Plant / Q000737:chemistry; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D020298:U937 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D016588:Anticarcinogenic Agents; C400423:Ciz1 protein, human; D009687:Nuclear Proteins; D002990:Clobetasol; D019833:Genistein