title: HDAC6 and SIRT2 promote bladder cancer cell migration and invasion by             targeting cortactin.
abstract: Histone deacetylase 6 (HDAC6) promotes cell motility and contributes to             the metastasis of cancers. The purpose of this study was to investigate the role             of HDAC6 in human bladder cancer for the first time. The results showed that HDAC6             promotes the migration and invasion of bladder cancer cells by targeting the cytoskeletal             protein cortactin. Furthermore, a colony formation assay as well as in vitro migration             and invasion assays demonstrated that this migration and invasion was suppressed             by the HDAC6-specific inhibitor tubacin. In addition, cortactin is the substrate             of SIRT2, which also belongs to the family of histone deacetylases. We demonstrated             that by using SIRT2-specific siRNA combined with tubacin treatment, the cell migratory             and invasive abilities were dramatically suppressed. Taken together, we conclude             that HDAC6 and SIRT2 work synergistically to promote cell migration and invasion             in bladder cancer, and the HDAC6-specific inhibitor tubacin may be regarded as             a novel therapeutic agent for bladder cancer.
authors.affiliation: Center for Laboratory Medicine, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Zuo
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000813:Anilides / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D051356:Cortactin / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D055785:Gene Knockdown Techniques / Q000379:methods; D000073864:Histone Deacetylase 6; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: D000813:Anilides; D051356:Cortactin; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D034741:RNA, Small Interfering; C474316:tubacin; C474199:SIRT2 protein, human; D056565:Sirtuin 2; C433811:HDAC6 protein, human; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases