title: Antioxidant activities of polysaccharides from Lentinus edodes and their significance for disease prevention.
abstract: The crude polysaccharide (LEP) was extracted by hot water from the fruiting bodies of Lentinus edodes, and further purified by DEAE-cellulose and Sepharose CL-6B chromatography, giving three polysaccharide fractions coded as LEPA1, LEPB1 and LEPC1. In this study, their chemical and physical characteristics of polysaccharide fractions and antioxidant capacities, including scavenging activity against hydroxyl radicals, superoxide radicals and Fe(2+)-chelating ability, were valuated. The results showed that LEPC1 exhibited significantly antioxidant activity at a concentration-dependent manner. Therefore these results indicated that the water-extractable polysaccharide fraction was a potent antioxidant and could be developed to be new health medicine for fighting against various human diseases.
authors.affiliation: School of Military Preventive Medicine, Fourth Military Medical University, Xi'an, China.
authors.forename: Huoliang
authors.initials: H
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Ju
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Yu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D004247:DNA / Q000737:chemistry; D006801:Humans; D017665:Hydroxyl Radical; D007501:Iron / Q000737:chemistry; D020074:Lentinula / Q000378:metabolism*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry; D020942:Shiitake Mushrooms; D013481:Superoxides / Q000737:chemistry; D014574:Uronic Acids / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D002614:Chelating Agents; D011134:Polysaccharides; D014574:Uronic Acids; D013481:Superoxides; D017665:Hydroxyl Radical; C043982:DEAE-Sepharose CL-6B; D004247:DNA; D012685:Sepharose; D007501:Iron