title: A comprehensive evaluation of colonic mucosal isolates of Sutterella wadsworthensis from inflammatory bowel disease.
abstract: Inflammatory bowel disease (IBD) arises in genetically susceptible individuals as a result of an unidentified environmental trigger, possibly a hitherto unknown bacterial pathogen. Twenty-six clinical isolates of Sutterella wadsworthensis were obtained from 134 adults and 61 pediatric patients undergoing colonoscopy, of whom 69 and 29 respectively had IBD. S. wadsworthensis was initially more frequently isolated from IBD subjects, hence this comprehensive study was undertaken to elucidate its role in IBD. Utilizing these samples, a newly designed PCR was developed, to study the prevalence of this bacterium in adult patients with ulcerative colitis (UC). Sutterella wadsworthensis was detected in 83.8% of adult patients with UC as opposed to 86.1% of control subjects (p = 0.64). Selected strains from IBD cases and controls were studied to elicit morphological, proteomic, genotypic and pathogenic differences. This study reports Scanning Electron Microscopy (SEM) appearances and characteristic MALDI-TOF MS protein profiles of S. wadsworthensis for the very first time. SEM showed that the bacterium is pleomorphic, existing in predominantly two morphological forms, long rods and coccobacilli. No differences were noted in the MALDI-TOF mass spectrometry proteomic analysis. There was no distinct clustering of strains identified from cases and controls on sequence analysis. Cytokine response after monocyte challenge with strains from patients with IBD and controls did not yield any significant differences. Our studies indicate that S. wadsworthensis is unlikely to play a role in the pathogenesis of IBD. Strains from cases of IBD could not be distinguished from those identified from controls.
authors.affiliation: Gastrointestinal Research Group, Division of Applied Medicine, University of Aberdeen, Foresterhill, Aberdeen, United Kingdom.
authors.forename: Indrani
authors.initials: I
authors.lastname: Mukhopadhya
authors.forename: Richard
authors.initials: R
authors.lastname: Hansen
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Nicholl
authors.forename: Yazeid A
authors.initials: YA
authors.lastname: Alhaidan
authors.forename: John M
authors.initials: JM
authors.lastname: Thomson
authors.forename: Susan H
authors.initials: SH
authors.lastname: Berry
authors.forename: Craig
authors.initials: C
authors.lastname: Pattinson
authors.forename: David A
authors.initials: DA
authors.lastname: Stead
authors.forename: Richard K
authors.initials: RK
authors.lastname: Russell
authors.forename: Emad M
authors.initials: EM
authors.lastname: El-Omar
authors.forename: Georgina L
authors.initials: GL
authors.lastname: Hold
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002648:Child; D015331:Cohort Studies; D003093:Colitis, Ulcerative / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D003106:Colon / Q000382:microbiology*; D003113:Colonoscopy; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005838:Genotype; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000235:genetics / Q000382:microbiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial
references.citation: J Biol Chem. 2011 May 20;286(20):17455-66
references.pmid: 21454518
references.citation: J Mol Diagn. 2009 Jan;11(1):75-86
references.pmid: 19095774
references.citation: J Gastroenterol. 2010 Mar;45(3):266-76
references.pmid: 20076977
references.citation: J Agric Food Chem. 2001 Nov;49(11):5679-84
references.pmid: 11714377
references.citation: FEMS Microbiol Ecol. 2002 Jan 1;39(1):33-9
references.pmid: 19709182
references.citation: Can J Gastroenterol. 2005 Sep;19 Suppl A:5A-36A
references.pmid: 16151544
references.citation: PLoS One. 2011 Feb 23;6(2):e17184
references.pmid: 21383845
references.citation: J Med Microbiol. 2010 Mar;59(Pt 3):295-301
references.pmid: 19959629
references.citation: J Clin Microbiol. 2000 Jan;38(1):286-91
references.pmid: 10618103
references.citation: Folia Microbiol (Praha). 2008;53(5):403-9
references.pmid: 19085074
references.citation: Nature. 2011 Jun 15;474(7351):307-17
references.pmid: 21677747
references.citation: Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1581-1584
references.pmid: 15388713
references.citation: Int J Syst Evol Microbiol. 2008 Apr;58(Pt 4):970-5
references.pmid: 18398204
references.citation: BMC Microbiol. 2011 Mar 15;11:53
references.pmid: 21406111
references.citation: Appl Environ Microbiol. 2009 Nov;75(22):7229-42
references.pmid: 19767470
references.citation: Clin Infect Dis. 1997 Sep;25 Suppl 2:S264-5
references.pmid: 9310700
references.citation: Methods Mol Biol. 2000;132:365-86
references.pmid: 10547847
references.citation: Int J Syst Bacteriol. 1996 Jan;46(1):252-8
references.pmid: 8573504
references.citation: Inflamm Bowel Dis. 2009 May;15(5):661-72
references.pmid: 19067417
references.citation: PLoS One. 2011;6(6):e21490
references.pmid: 21738679
references.citation: J Clin Microbiol. 2009 Feb;47(2):453-5
references.pmid: 19052183
references.citation: J Agric Food Chem. 2009 Aug 12;57(15):6485-501
references.pmid: 19580283
references.citation: Dig Dis. 2009;27 Suppl 1:99-103
references.pmid: 20203504
references.citation: Inflamm Bowel Dis. 2006 Feb;12(2):106-11
references.pmid: 16432374
references.citation: Gastroenterology. 1998 Apr;114(4):755-63
references.pmid: 9516396
references.citation: Brief Bioinform. 2004 Jun;5(2):150-63
references.pmid: 15260895
references.citation: FEMS Microbiol Ecol. 2004 Oct 1;50(1):25-36
references.pmid: 19712374
references.citation: Br Med J. 1977 Jul 2;2(6078):9-11
references.pmid: 871765