title: [Insertion of glycoprotein gene between P and M gene influences the pathogenicity of the rabies virus Flury LEP].
abstract: OBJECTIVE
  To study the biological characteristics and pathogenicity of a recombinant rabies virus Flury LEP (low egg passage) that has two glycoprotein genes (G gene).
  
  
  METHODS
  By using reverse genetics techniques, we constructed a recombinant virus Flury LEP that has an additional G gene between P and M gene (rLEP-PGM). Then we studied the biological characteristics of the recombinant virus and its pathogenicity on mice.
  
  
  RESULTS
  The in vitro growth characteristic of rLEP-PGM were similar to the LEP strain. Western blot analysis of glycoprotein expression showed that the glycoprotein expression level of rLEP-PGM was 1.5 times higher than LEP. The LD50 of rLEP-PGM and LEP was 3 FFU and 1 FFU by intracerebral injection. However, the LD50 of intramuscular injection was 4 x 10(4) Lg FFU and 3.2 x 10(5) Lg FFU, respectively.
  
  
  CONCLUSIONS
  Insertion of an additional G gene between P and M gene can significantly raise the expression level of glycoprotein and enhance the ability to invade central nervous system from peripheral sites.
authors.affiliation: Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China. zhaihongyue@sohu.com
authors.forename: Hongyue
authors.initials: H
authors.lastname: Zhai
authors.forename: Lihong
authors.initials: L
authors.lastname: Tao
authors.forename: Jinying
authors.initials: J
authors.lastname: Ge
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.forename: Lei
authors.initials: L
authors.lastname: Shuai
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D011820:Rabies virus / Q000235:genetics / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus