title: Matrix metalloproteinase-8 expression in periodontal tissues surgically removed from diabetic and non-diabetic patients with periodontal disease.
abstract: BACKGROUND
  Although it is known that periodontal matrix metalloproteinase-8 (MMP-8) expression is associated with periodontal disease, the information concerning the periodontal MMP-8 expression in diabetic patients with periodontal disease is insufficient.
  
  
  METHODS
  Periodontal tissue specimens were collected from seven patients without periodontal disease and diabetes (Group 1), 15 patients with periodontal disease alone (Group 2) and 10 patients with both periodontal disease and diabetes (Group 3). The frozen sections were prepared and MMP-8 protein expression was detected using immunohistochemistry and quantified. For in vitro study, human U937 mononuclear cells were pre-exposed to normal or high glucose and then treated with lipopolysaccharide (LPS).
  
  
  RESULTS
  The nonparametric Kruskal-Wallis test showed that the difference in MMP-8 protein levels among the three groups were statistically significant (p = 0.003). Nonparametric analysis using Jonckheere-Terpstra test showed a tendency of increase in periodontal MMP-8 levels across Group 1 to Group 2 to Group 3 (p = 0.0002). In vitro studies showed that high glucose and LPS had a synergistic effect on MMP-8 expression.
  
  
  CONCLUSIONS
  Our current study showed an increasing trend in MMP-8 protein expression levels across patients without both periodontal disease and diabetes, patients with periodontal disease alone and patients with both diseases.
authors.affiliation: Division of Periodontics, Department of Stomatology, College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Hardy
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Ross
authors.forename: Corinne A
authors.initials: CA
authors.lastname: Schuyler
authors.forename: Renata S
authors.initials: RS
authors.lastname: Leite
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Slate
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000201:enzymology*; D005006:Ethnicity; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005891:Gingivitis / Q000150:complications / Q000201:enzymology*; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010518:Periodontitis / Q000150:complications / Q000201:enzymology*; D010519:Periodontium / Q000201:enzymology; D018709:Statistics, Nonparametric; D020298:U937 Cells
chemical_list: D008070:Lipopolysaccharides; D020784:Matrix Metalloproteinase 8
references.citation: Periodontol 2000. 2003;31:77-104
references.pmid: 12656997
references.citation: J Periodontal Res. 2007 Feb;42(1):31-8
references.pmid: 17214637
references.citation: J Periodontol. 1997 Feb;68(2):127-35
references.pmid: 9058329
references.citation: Ann N Y Acad Sci. 1999 Jun 30;878:130-40
references.pmid: 10415725
references.citation: J Periodontal Res. 2000 Oct;35(5):259-65
references.pmid: 11005153
references.citation: J Pathol. 2001 Jun;194(2):232-8
references.pmid: 11400153
references.citation: Ann Periodontol. 1998 Jul;3(1):51-61
references.pmid: 9722690
references.citation: Ann Periodontol. 1998 Jul;3(1):40-50
references.pmid: 9722689
references.citation: J Clin Periodontol. 1996 Dec;23(12):1127-32
references.pmid: 8997658
references.citation: J Periodontol. 2006 Nov;77(11):1803-8
references.pmid: 17076603
references.citation: J Periodontol. 1999 Aug;70(8):935-49
references.pmid: 10476904
references.citation: Periodontol 2000. 2007;43:245-53
references.pmid: 17214842
references.citation: Oral Dis. 2004 Nov;10(6):311-8
references.pmid: 15533204
references.citation: J Periodontal Res. 2010 Oct;45(5):688-94
references.pmid: 20682019
references.citation: J Cell Biochem. 2010 May;110(1):248-59
references.pmid: 20225236
references.citation: J Biol Chem. 2010 Feb 5;285(6):3916-3927
references.pmid: 20007708
references.citation: J Am Soc Nephrol. 2004 Nov;15(11):2792-800
references.pmid: 15504932
references.citation: J Periodontol. 2010 Mar;81(3):384-91
references.pmid: 20192865
references.citation: J Clin Periodontol. 2005 Mar;32(3):310-6
references.pmid: 15766376
references.citation: Ann Periodontol. 1999 Dec;4(1):32-8
references.pmid: 10863373
references.citation: Int J Obes Relat Metab Disord. 2002 Jul;26(7):994-1008
references.pmid: 12080455
references.citation: J Endocrinol. 2008 Jan;196(1):45-55
references.pmid: 18180316
references.citation: J Periodontal Res. 2003 Aug;38(4):436-9
references.pmid: 12828663
references.citation: J Biol Chem. 2009 May 15;284(20):13714-13724
references.pmid: 19307187
references.citation: J Periodontal Res. 2006 Dec;41(6):503-12
references.pmid: 17076774
references.citation: Arkh Patol. 2002 May-Jun;64(3):47-53
references.pmid: 15338725
references.citation: Int J Cancer. 1976 May 15;17(5):565-77
references.pmid: 178611
references.citation: J Periodontal Res. 2004 Dec;39(6):415-23
references.pmid: 15491346
references.citation: J Periodontal Res. 2008 Dec;43(6):717-22
references.pmid: 18627438
references.citation: J Periodontol. 1999 Nov;70(11):1292-302
references.pmid: 10588492
references.citation: PLoS Pathog. 2010 Apr 29;6(4):e1000874
references.pmid: 20442866
references.citation: J Periodontal Res. 1995 Jan;30(1):23-33
references.pmid: 7722844
references.citation: J Histochem Cytochem. 1997 Nov;45(11):1559-65
references.pmid: 9358857
references.citation: J Pathol. 2001 Jun;194(2):217-24
references.pmid: 11400151
references.citation: Oral Dis. 2011 Jan;17(1):115-22
references.pmid: 20659259
references.citation: Oral Dis. 2010 Jul;16(5):476-81
references.pmid: 20233316
references.citation: J Periodontol. 2005 Nov;76(11 Suppl):2075-84
references.pmid: 16277579