title: Additional paper waste in pulping sludge for biohydrogen production by heat-shocked sludge.
abstract: Dark anaerobic fermentation is an interesting alternative method for producing biohydrogen (H(2)) as a renewable fuel because of its low cost and various usable organic substrates. Pulping sludge from wastewater treatment containing plentiful cellulosic substrate could be feasibly utilized for H(2) production by dark fermentation. The objective of this study was to investigate the optimal proportion of pulping sludge to paper waste, the optimal initial pH, and the optimal ratio of carbon and nitrogen (C/N) for H(2) production by anaerobic seed sludge pretreated with heat. The pulping sludge was pretreated with NaOH solution at high temperature and further hydrolyzed with crude cellulase. Pretreatment of the pulping sludge with 3% NaOH solution under autoclave at 121 °C for 2 h, hydrolysis with 5 FPU crude cellulase at 50 °C, and pH 4.8 for 24 h provided the highest reducing sugar production yield (229.68 ± 2.09 mg/g(TVS)). An initial pH of 6 and a C/N ratio of 40 were optimal conditions for H(2) production. Moreover, the supplement of paper waste in the pulping sludge enhanced the cumulative H(2) production yield. The continuous hydrogen production was further conducted in a glass reactor with nylon pieces as supporting media and the maximum hydrogen production yield was 151.70 ml/g(TVS).
authors.affiliation: Environmental Technology Unit, Department Environmental Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. fscippck@ku.ac.th
authors.forename: Prapaipid
authors.initials: P
authors.lastname: Chairattanamanokorn
authors.forename: Supachok
authors.initials: S
authors.lastname: Tapananont
authors.forename: Siriporn
authors.initials: S
authors.lastname: Detjaroen
authors.forename: Juthatip
authors.initials: J
authors.lastname: Sangkhatim
authors.forename: Patana
authors.initials: P
authors.lastname: Anurakpongsatorn
authors.forename: Pramote
authors.initials: P
authors.lastname: Sirirote
mesh_terms: D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste; D009584:Nitrogen / Q000378:metabolism; D010209:Paper; D012722:Sewage; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D007220:Industrial Waste; D012722:Sewage; D055549:Volatile Organic Compounds; D002244:Carbon; D006859:Hydrogen; D009584:Nitrogen