title: [Determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta by RP-HPLC].
abstract: OBJECTIVE
  To develop a RP-HPLC method for determining the contents of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta.
  
  
  METHODS
  A Kromasil-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of acetonitrile -1% acetic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 280 nm.
  
  
  RESULTS
  The correlation coefficients of atranol, lecanorin, ethyl orsellinate, and methyl orsellinate were higher than 0.999. Recoveries were from 102.9% to 95.30%; with RSD from 2.3% to 1.9%.
  
  
  CONCLUSIONS
  The method is quick, simple and repeatable for simultaneous determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in U. diffracta.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources & Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Huanyang
authors.initials: H
authors.lastname: Qi
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Yanping
authors.initials: Y
authors.lastname: Shi
mesh_terms: D001547:Benzaldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012118:Resorcinols / Q000032:analysis*; D020949:Usnea / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D012118:Resorcinols; C493895:atranol; C472106:methyl orsellinate