title: Natural biopolymer for preservation of microorganisms during sampling and storage.
abstract: Stability of microbial cultures during sampling and storage is a vital issue in various fields of medicine, biotechnology, food science, and forensics. We have developed a unique bacterial preservation process involving a non-toxic, water-soluble acacia gum polymer that eliminates the need for refrigerated storage of samples. The main goal of this study is to characterize the efficacy of acacia gum polymer for preservation of pathogenic bacteria (Bacillus anthracis and methicillin-resistant Staphylococcus aureus-MRSA) on different materials, used for swabbing and filtration: cotton, wool, polyester, rayon, charcoal cloth, and Whatman paper. Acacia gum polymer used for preservation of two pathogens has been shown to significantly protect bacteria during dehydration and storage in all tested samples at the range of temperatures (5-45°C for MRSA and 40-90°C for B. anthracis). Our results showed higher recovery as well as higher viability during the storage of both bacteria in all materials with acacia gum. Addition of acacia gum polymer to swabbing materials or filters will increase efficacy of sample collection and identification of pathogenic bacteria from locations such as hospitals or the environment. Proposed approach can also be used for long-term storage of culture collections, since acacia gum contributes to viability and stability of bacterial cultures.
authors.affiliation: Department of Anatomy, Physiology, and Pharmacology Auburn University, Auburn, AL 36849, USA.
authors.forename: Iryna
authors.initials: I
authors.lastname: Sorokulova
authors.forename: James
authors.initials: J
authors.lastname: Watt
authors.forename: Eric
authors.initials: E
authors.lastname: Olsen
authors.forename: Ludmila
authors.initials: L
authors.lastname: Globa
authors.forename: Timothy
authors.initials: T
authors.lastname: Moore
authors.forename: James
authors.initials: J
authors.lastname: Barbaree
authors.forename: Vitaly
authors.initials: V
authors.lastname: Vodyanoy
mesh_terms: D001408:Bacillus anthracis / Q000737:chemistry* / Q000254:growth & development; D001704:Biopolymers / Q000737:chemistry*; D006170:Gum Arabic / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000737:chemistry* / Q000254:growth & development; D011309:Preservation, Biological / Q000295:instrumentation / Q000379:methods*; D013048:Specimen Handling; D013696:Temperature
chemical_list: D001704:Biopolymers; D006170:Gum Arabic