title: [Analysis of microsatellite instability in laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  The literature on the involvement of microsatellite instability in head and neck squamous cell carcinoma shows great variability, probably due to differences in the testing methods. Using a consensus detection system, we aimed to reach a reliable estimate of microsatellite instability prevalence in a subset of head and neck squamous cell carcinoma cases.
  
  
  METHODS
  The microsatellite instabilityI status of 43 patients with previously untreated primary laryngeal squamous cell carcinomas was analyzed by a multiplex polymerase chain reaction assay including 5 mononucleotide repeat markers.
  
  
  RESULTS
  Thirty-six cases showed a stable phenotype or a microsatellite stable phenotype (83.7%) and 7 cases (16.3%) showed an microsatellite instability-positive phenotype. One case showed instability in 3 of 5 markers, 1 case in 2 markers and 5 cases in 1 marker. The microsatellite instability-positive and stable cases did not differ with respect to age, tumour stage, lymph node or distant metastases.
  
  
  CONCLUSIONS
  Our data showed that a proportion of laryngeal squamous cell carcinomas are microsatellite instability positive. Knowledge of microsatellite instability patient status will allow adjusting anticancer therapy at an individual level.
authors.affiliation: Departamento de Otorrinolaringología, IUOPA, Hospital Universitario Central de Asturias, Oviedo, España. xurde_garcia@hotmail.com
authors.forename: Jorge
authors.initials: J
authors.lastname: García Martínez
authors.forename: Jhudit
authors.initials: J
authors.lastname: Pérez-Escuredo
authors.forename: Dario
authors.initials: D
authors.lastname: García-Carracedo
authors.forename: Marta
authors.initials: M
authors.lastname: Alonso-Guervós
authors.forename: Carlos
authors.initials: C
authors.lastname: Suárez-Nieto
authors.forename: José Luis
authors.initials: JL
authors.lastname: Llorente-Pendás
authors.forename: César
authors.initials: C
authors.lastname: Alvarez-Marcos
authors.forename: Mario
authors.initials: M
authors.lastname: Hermsen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery; D002454:Cell Differentiation; D003131:Combined Modality Therapy; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004273:DNA, Neoplasm