title: Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China.
abstract: A new isolate of canine distemper virus (CDV), named ZJ7, was isolated from lung tissues of a dog suspected with CDV infection using MDCK cells. The ZJ7 isolate induced cytopathogenic effects of syncytia in MDCK cell after six passages. In order to evaluate pathogenesis of ZJ7 strain, three CDV sero-negative dogs were intranasally inoculated with its virus suspension. All infected dogs developed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, fever and weight loss at 21 dpi, whereas the mock group infected with DMEM were normal. The results demonstrated that CDV-ZJ7 strain isolated by MDCK cell was virulent, and the nucleotide and amino acid sequences of strain ZJ7 had no change after isolation by MDCK cell when compared with the original virus from the fresh tissues. Molecular and phylogenetic analyses for the nucleocapsid (N), phosphoprotein (P) and receptor binding haemagglutinin (H) gene of the ZJ7 isolate clearly showed it is joins to the Asia 1 group cluster of CDV strains, the predominant genotype in China.
authors.affiliation: Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, State Key Laboratory of Special Economic Animal Molecular Biology, Chinese Academy of Agricultural Sciences CAAS, 15 Luming Street, Jilin 132109, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tan
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wen
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Wang
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Xiu-Dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Hu
authors.forename: Xin-Chuan
authors.initials: XC
authors.lastname: Shi
authors.forename: Bo-Chao
authors.initials: BC
authors.lastname: Yang
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Chen
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Cheng
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D016000:Cluster Analysis; D003588:Cytopathogenic Effect, Viral; D004216:Distemper / Q000473:pathology / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004285:Dogs; D005260:Female; D005838:Genotype; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
references.citation: Vet Microbiol. 2010 Jan 6;140(1-2):34-42
references.pmid: 19647380
references.citation: J Vet Diagn Invest. 1994 Jul;6(3):277-88
references.pmid: 7948195
references.citation: J Gen Virol. 1990 Sep;71 ( Pt 9):2085-92
references.pmid: 1698923
references.citation: J Vet Med Sci. 2004 Feb;66(2):175-81
references.pmid: 15031546
references.citation: Acta Vet Scand. 2009 Oct 16;51:38
references.pmid: 19835588
references.citation: Proc Soc Exp Biol Med. 1967 Nov;126(2):571-4
references.pmid: 6079942
references.citation: Microbiol Immunol. 1988;32(2):235-40
references.pmid: 2836715
references.citation: J Vet Diagn Invest. 1992 Jul;4(3):258-63
references.pmid: 1387554
references.citation: Arch Virol. 1993;132(3-4):421-8
references.pmid: 7691048
references.citation: Vet Microbiol. 2007 Dec 15;125(3-4):341-9
references.pmid: 17628358
references.citation: J Comp Pathol. 1971 Jan;81(1):49-54
references.pmid: 5090886
references.citation: Virol J. 2011 Feb 27;8:85
references.pmid: 21352564
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Am J Vet Res. 1980 Feb;41(2):284-92
references.pmid: 6989302
references.citation: Vet Microbiol. 2007 May 16;122(1-2):32-42
references.pmid: 17275219
references.citation: J Virol Methods. 2004 Jun 15;118(2):147-57
references.pmid: 15081610
references.citation: Vet Microbiol. 2006 Sep 10;116(4):301-9
references.pmid: 16730927
references.citation: J Gen Virol. 1997 Feb;78 ( Pt 2):367-72
references.pmid: 9018059
references.citation: J Virol. 2001 Jul;75(14):6418-27
references.pmid: 11413309
references.citation: Wei Sheng Wu Xue Bao. 2008 Jul;48(7):986-91
references.pmid: 18837382
references.citation: J Gen Virol. 1986 Mar;67 ( Pt 3):487-503
references.pmid: 2419490
references.citation: J Comp Pathol. 1995 Aug;113(2):185-90
references.pmid: 8543675
references.citation: Vet Microbiol. 1995 May;44(2-4):187-91
references.pmid: 8588312
references.citation: Microbiol Immunol. 1986;30(10):1067-73
references.pmid: 3796316
references.citation: Am J Vet Res. 1973 Nov;34(11):1459-63
references.pmid: 4201293
references.citation: Infect Immun. 1980 Sep;29(3):940-4
references.pmid: 7429639
references.citation: Infect Immun. 1975 May;11(5):1069-78
references.pmid: 1091560
references.citation: J Gen Virol. 1997 Feb;78 ( Pt 2):373-80
references.pmid: 9018060