title: Yeast mRNA cap-binding protein Cbc1/Sto1 is necessary for the rapid reprogramming of translation after hyperosmotic shock.
abstract: In response to osmotic stress, global translation is inhibited, but the mRNAs encoding stress-protective proteins are selectively translated to allow cell survival. To date, the mechanisms and factors involved in the specific translation of osmostress-responsive genes in Saccharomyces cerevisiae are unknown. We find that the mRNA cap-binding protein Cbc1 is important for yeast survival under osmotic stress. Our results provide new evidence supporting a role of Cbc1 in translation initiation. Cbc1 associates with polysomes, while the deletion of the CBC1 gene causes hypersensitivity to the translation inhibitor cycloheximide and yields synthetic "sickness" in cells with limiting amounts of translation initiator factor eIF4E. In cbc1Δ mutants, translation drops sharply under osmotic stress, the subsequent reinitiation of translation is retarded, and "processing bodies" containing untranslating mRNAs remain for long periods. Furthermore, osmostress-responsive mRNAs are transcriptionally induced after osmotic stress in cbc1Δ cells, but their rapid association with polysomes is delayed. However, in cells containing a thermosensitive eIF4E allele, their inability to grow at 37ºC is suppressed by hyperosmosis, and Cbc1 relocalizes from nucleus to cytoplasm. These data support a model in which eIF4E-translation could be stress-sensitive, while Cbc1-mediated translation is necessary for the rapid translation of osmostress-protective proteins under osmotic stress.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universitat de València, Burjassot, Valencia, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Garre
authors.forename: Lorena
authors.initials: L
authors.lastname: Romero-Santacreu
authors.forename: Nikki
authors.initials: N
authors.lastname: De Clercq
authors.forename: Nati
authors.initials: N
authors.lastname: Blasco-Angulo
authors.forename: Per
authors.initials: P
authors.lastname: Sunnerhagen
authors.forename: Paula
authors.initials: P
authors.lastname: Alepuz
mesh_terms: D000222:Adaptation, Physiological; D002467:Cell Nucleus / Q000378:metabolism; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D050296:Microbial Viability; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D009997:Osmotic Pressure; D011132:Polyribosomes / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D021381:Protein Transport; D039381:RNA Cap-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D009687:Nuclear Proteins; D039381:RNA Cap-Binding Proteins; D012333:RNA, Messenger; C101398:STO1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
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