title: [Primary detection of biological functions of anti-hGITR(aa27-165);PcAb].
abstract: OBJECTIVE
  To detect the rabbit-derived polyclonal antibodies against extracellular protein segments of human glucocorticoid-inducible tumor necrosis factor receptors (anti-hGITR(aa27-165);PcAb) with regard to its capacity of linkage to natural GITR molecules and the function on CD4(+); T cells.
  
  
  METHODS
  Human peripheral blood mononuclear cells (PBMCs) were isolated and cultured under stimulation;  flow cytometry was applied to check the capacity of anti-hGITR(aa27-165);PcAb for linkage to natural GITR molecules on PBMCs;  human CD4(+); T cells were isolated by immunological magnetic beads and (3);H-TdR incorporation tests were performed to observe improving-proliferation action of anti-hGITR(aa27-165);PcAb while CD4(+); T cells were cultured with or not with some cytokines.
  
  
  RESULTS
  Anti-hGITR(aa27-165);PcAb was able to bind GITR molecules with natural conformation in a concentration-dependent way;  furthermore,  this PcAb could improve the reproduction of CD4(+); T cells.
  
  
  CONCLUSIONS
  The rabbit-derived anti-hGITR(aa27-165);PcAb prepared in our laboratory is capable of linking to natural target molecules and possesses the activation function upon CD4(+); T cells,  the further exploration should allow for its applications for diagnosis and treatment of relavent diseases.
authors.affiliation: The Clinical Laboratory of the Affiliated People's Hospital,  Zhenjiang 212002,  China.
authors.forename: Ling
authors.initials: L
authors.lastname: Cai
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Qing-zhen
authors.initials: QZ
authors.lastname: Han
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Chen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology* / Q000302:isolation & purification; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D053450:Glucocorticoid-Induced TNFR-Related Protein / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D011817:Rabbits
chemical_list: D000906:Antibodies; D053450:Glucocorticoid-Induced TNFR-Related Protein