title: Glycolate and 2-phosphoglycolate content of tissues measured by ion chromatography coupled to mass spectrometry.
abstract: Glycolate and 2-phosphoglycolate (PG) are 2-carbon monocarboxylic acids with ill-defined metabolic roles. Their concentrations have not yet been described in tissues apart from body fluids and erythrocytes. We describe the use of ion chromatography coupled with mass spectrometry (IC-MS) to quantify levels of glycolate and PG in tissue. Sample preparation and analysis can be performed within an hour. Low concentrations of glycolate (12-48 nmol/g) and PG (4-17 nmol/g) were detected in all tissues. The availability of this IC-MS assay will facilitate investigations of the origin, function, and metabolism of glycolate and PG in tissues.
authors.affiliation: Department of Urology, Wake Forest University Medical Center, Winston-Salem, NC 27157, USA. jknight@wfubmc.edu
authors.forename: John
authors.initials: J
authors.lastname: Knight
authors.forename: Mark
authors.initials: M
authors.lastname: Hinsdale
authors.forename: Ross
authors.initials: R
authors.lastname: Holmes
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry; D006016:Glycolates / Q000032:analysis*; D006801:Humans; D051379:Mice; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D006016:Glycolates; C031149:glycolic acid; C013193:phosphoglycolate
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