title: [Construction of a bivalent plant expression vector carrying VvSUC11 and VvSUC12 genes and its genetic transformation in sugar beet].
abstract: We have recombined genes VvSUC11, VvSUC12 from Vitis vinifera L., and root-specific promoters of sweet potato storage protein gene from Ipomoea batatas L. Lam., named as SP1 and SP2. We have constructed a vector pCAMBIA2301-SP1- VvSUC11-SP2-VvSUC12 using pCAMBIA2301 as an original vector. VvSUC11 and VvSUC12 were under the control of root-specific promoters of sweet potato storage protein gene. We transformed the vector into KWS-9103 breeding line of Beta vulgaris L. with Agrobacterium-mediated transformation. We have established the optimal genetic transformation protocol of sugar beet as following: the explants pre-cultured for 4 days were immersed in Agrobacterium suspension of OD(600)=0.5, supplemented with 0.005% Silwet L-77, and followed by a 4-day culture on medium containing cefotaxime, then the buds were selected on medium containing kanamycin and cefotaxime. The percentage of kanamycin-resistant buds was as high as 42%. Results of PCR and RT-PCR proved that the target genes had integrated into sugar beet genome and expressed. It will lay a foundation for further studying their function in Beta vulgaris.
authors.affiliation: Key Laboratory of Agricultural Biotechnology, Shihezi University, Shihezi 832000, China.
authors.forename: Donglin
authors.initials: D
authors.lastname: Yin
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhu
authors.forename: Aiying
authors.initials: A
authors.lastname: Wang
authors.forename: Benchun
authors.initials: B
authors.lastname: Xiang
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D027461:Beta vulgaris / Q000235:genetics*; D005822:Genetic Vectors; D027723:Ipomoea batatas / Q000235:genetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D027843:Vitis / Q000235:genetics*
chemical_list: D026901:Membrane Transport Proteins; D010940:Plant Proteins; C067142:sporamin protein, Ipomoea batatas; C078729:sucrose transport protein, plant