title: Lifestyle modification induced weight loss and changes of cardiometabolic risk factors including lowering of inflammatory response in obese children.
abstract: OBJECTIVE
  Obesity is associated with increased inflammation which represents a link to atherosclerosis and cardiovascular disease. Lipoprotein associated phospholipase A2 (Lp-PLA2) is an independent marker of inflammation and atherosclerosis risk. To assess the impact of weight loss on metabolic markers of atherosclerosis including Lp-PLA2 we examined a group of Czech non-diabetic obese/overweight children exposed to a lifestyle intervention.
  
  
  METHODS
  Fourty unrelated overweight/obese non-diabetic Czech children (13.7 ± 2.1 years, average BMI at baseline 29.8 ± 2.6 kg/m2) underwent 4 weeks of lifestyle modification (reduction of energy intake to age matched optimum and supervised physical activity). Anthropometrical and biochemical variables were determined at baseline and after the intervention. Lp-PLA2 mass concentration was assessed using the ELISA kit. Wilcocson's rank test and Spearman's correlation were used for statistical analysis.
  
  
  RESULTS
  A significant decrease of BMI and waist circumference was associated with significant changes of plasma lipoprotein and glycaemia levels. Mass concentration of Lp-PLA2 at the baseline was 402 ± 94 μg/ml, after the intervention 368 ± 105 μg/ml (p=0.008). Change in Lp-PLA2 was associated with triglyceride level decrease (p=0.009).
  
  
  CONCLUSIONS
  Intensive lifestyle modification leading to body weight decrease results in significant changes of plasma lipoprotein levels and, also, a drop of Lp-PLA2 levels in paediatric obese patients. However, even after the intervention Lp-PLA2 concentrations in this patient group remain elevated suggesting possible increased atherosclerosis risk in later life.
authors.affiliation: Third Department of Internal Medicine, General University Hospital and 1st Medical Faculty of Charles University in Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Motykova
authors.forename: Lukáš
authors.initials: L
authors.lastname: Zlatohlavek
authors.forename: Martina
authors.initials: M
authors.lastname: Prusikova
authors.forename: Vera
authors.initials: V
authors.lastname: Lanska
authors.forename: Richard
authors.initials: R
authors.lastname: Ceska
authors.forename: Ludmila
authors.initials: L
authors.lastname: Vasickova
authors.forename: Michal
authors.initials: M
authors.lastname: Vrablik
mesh_terms: D050154:Adiposity / Q000502:physiology; D000293:Adolescent; D000886:Anthropometry; D050197:Atherosclerosis / Q000453:epidemiology; D015992:Body Mass Index; D001835:Body Weight / Q000502:physiology; D002318:Cardiovascular Diseases / Q000453:epidemiology*; D002648:Child; D002149:Energy Intake; D005260:Female; D006801:Humans; D008019:Life Style; D008055:Lipids / Q000097:blood; D008297:Male; D008659:Metabolic Diseases / Q000453:epidemiology*; D009765:Obesity / Q000534:rehabilitation*; D050177:Overweight / Q000534:rehabilitation; D012307:Risk Factors; D055105:Waist Circumference / Q000502:physiology; D015431:Weight Loss / Q000502:physiology*
chemical_list: D008055:Lipids