title: Dissecting T-cell activation with high-resolution live-cell microscopy.
abstract: Results from live-cell microscopy suggest that the behaviour of isolated components of the T-cell activation machinery in vitro does not represent the reality inside cells. Understanding the cellular-scale dynamics of microcluster migration can only be accomplished by in situ observation. Developments in 'super-resolution' microscopy have permitted investigators to move beyond tracking the movements of individual molecules, allowing the recognition of protein islands and nanodomains present in quiescent and active T cells. Many high-resolution techniques have their own susceptibilities to artefacts, so it is important to take a multifaceted approach to confirm results. A major challenge for the future will be to integrate all the new information into a coherent model of antigen recognition and T-cell activation.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Illingworth
authors.forename: P
authors.initials: P
authors.lastname: Anton van der Merwe
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008051:Lipid Bilayers; D008213:Lymphocyte Activation / Q000276:immunology*; D008285:Major Histocompatibility Complex; D008856:Microscopy, Fluorescence / Q000379:methods*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D008051:Lipid Bilayers; D011948:Receptors, Antigen, T-Cell
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