title: Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations.
abstract: BACKGROUND
  Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with aneuploidy and aggressive phenotype.
  
  
  RESULTS
  We investigated the centrosome status and mitotic abnormalities in three thyroid carcinoma-derived cell lines, each maintaining the specific, biologically relevant gene alteration harbored by the parental tumors: RET/PTC1 rearrangement in TPC1; heterozygous and homozygous BRAFV600E mutation in K1 and in B-CPAP, respectively. B-CPAP cells showed a statistically significant (P < 0.01) higher frequency of abnormal mitotic figures compared to TPC1 and K1 cells.
  
  
  CONCLUSIONS
  Our data indicate that RET/PTC1 oncogenic activity is not related to mitotic chromosome impairment and missegregation whereas, based on the consistent difference in types/frequencies of centrosome and spindle abnormalities observed between K1 and B-CPAP cells, the hetero/homozygous allelic status of BRAFV600E mutation seems to be not irrelevant in respect to chromosomal instability development.
authors.affiliation: Dipartimento per lo Studio del Territorio e delle sue Risorse, Università degli Studi di Cagliari, Genova, 16132, Italy.|IRCCS Azienda Ospedaliera Universitaria San Martino - IST - Istituto Nazionale per la Ricerca sul Cancro, Genova, 16132, Italy.
authors.forename: Irena
authors.initials: I
authors.lastname: Maric
authors.affiliation: Dipartimento per lo Studio del Territorio e delle sue Risorse, Università degli Studi di Cagliari, Genova, 16132, Italy.|IRCCS Azienda Ospedaliera Universitaria San Martino - IST - Istituto Nazionale per la Ricerca sul Cancro, Genova, 16132, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Viaggi
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università di Cagliari, 09042, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Caria
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università di Cagliari, 09042, Italy.
authors.forename: Daniela V
authors.initials: DV
authors.lastname: Frau
authors.affiliation: IRCCS Azienda Ospedaliera Universitaria San Martino - IST - Istituto Nazionale per la Ricerca sul Cancro, Genova, 16132, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Degan
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università di Cagliari, 09042, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Vanni
references.citation: Br J Cancer. 1989 Dec;60(6):897-903
references.pmid: 2557880
references.citation: Virchows Arch. 2009 Aug;455(2):149-57
references.pmid: 19588164
references.citation: J Clin Endocrinol Metab. 2006 Jun;91(6):2414-23
references.pmid: 16595592
references.citation: Cancer Genet Cytogenet. 2010 Nov;203(1):21-9
references.pmid: 20951315
references.citation: Biochim Biophys Acta. 2009 Apr;1795(2):92-103
references.pmid: 19167460
references.citation: Hum Mol Genet. 2006 Mar 15;15(6):831-8
references.pmid: 16446310
references.citation: Biochim Biophys Acta. 2008 Sep;1786(1):4-14
references.pmid: 18703117
references.citation: Cancer Res. 2008 Sep 1;68(17):7156-64
references.pmid: 18757431
references.citation: Endocr Relat Cancer. 2006 Jun;13(2):455-64
references.pmid: 16728573
references.citation: Trends Cell Biol. 2001 Jan;11(1):18-21
references.pmid: 11146294
references.citation: Biochem Biophys Res Commun. 1990 Apr 30;168(2):402-8
references.pmid: 2334411
references.citation: Leukemia. 2003 Nov;17(11):2207-13
references.pmid: 14523473
references.citation: Trends Genet. 2008 Sep;24(9):457-66
references.pmid: 18675487
references.citation: Nat Rev Cancer. 2007 Dec;7(12):911-24
references.pmid: 18004399
references.citation: Nat Rev Endocrinol. 2011 Aug 30;7(10):569-80
references.pmid: 21878896
references.citation: Nature. 2002 Jun 27;417(6892):949-54
references.pmid: 12068308
references.citation: Oncogene. 2007 May 3;26(20):2939-44
references.pmid: 17072342
references.citation: Curr Biol. 2006 Mar 21;16(6):564-9
references.pmid: 16546079
references.citation: Cancer Res. 2010 Jan 15;70(2):675-84
references.pmid: 20068179
references.citation: Oncol Rep. 2007 Oct;18(4):917-26
references.pmid: 17786355
references.citation: Cancer Genet Cytogenet. 2004 Jun;151(2):171-7
references.pmid: 15172756
references.citation: Thyroid. 2007 Aug;17(8):707-15
references.pmid: 17725429
references.citation: J Transl Med. 2010 Jul 14;8:67
references.pmid: 20630094
references.citation: BMC Cancer. 2008 Dec 16;8:371
references.pmid: 19087340
references.citation: J Clin Endocrinol Metab. 2011 Apr;96(4):E577-85
references.pmid: 21289267
references.citation: Cancer Res. 2005 Mar 15;65(6):2465-73
references.pmid: 15781663