title: Involvement of leucine zipper transcription factor-like protein 1 (Lztfl1) in the attenuation of cognitive impairment by exercise training.
abstract: It is well known that exercise prevents and reduces cognitive impairment. In the present study, we focused on exercise training as a tool to prevent cognitive impairment, and searched for novel molecules that may relate to the prevention of cognitive impairment in the hippocampus. Two-month-old senescence-accelerated mouse prone-8 (SAMP8) mice were subjected to voluntary exercise training by running on a wheel for 4 months, and were then assigned a conditioned fear memory test. Moreover, various mRNA levels in the hippocampus were examined by DNA array analysis and real-time PCR. Contextual fear memory in SAMP8 control mice was significantly impaired compared with that in non-senescence mice. Exercise training definitely attenuated such cognitive impairment. The results of real-time PCR analysis that was conducted following DNA array analysis in the hippocampus revealed that, compared with SAMR8 control mice, the expression levels of leucine zipper transcription factor-like protein 1 (Lztfl1) mRNA were significantly higher in SAMP8 mice subjected to exercise training. In addition, the overexpression of Lztfl1 promoted neurite outgrowth in Neuro 2a cells. These results suggest that exercise has a preventive effect on cognitive impairment in SAMP8 mice, and that exercise-induced increase in Lztfl1 induces neurite outgrowth.
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan. sakutaku@ks.kyorin-u.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Junetsu
authors.initials: J
authors.lastname: Ogasawara
authors.forename: Takako
authors.initials: T
authors.lastname: Kizaki
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Kimio
authors.initials: K
authors.lastname: Akagawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Izawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Oh-ishi
authors.forename: Shukoh
authors.initials: S
authors.lastname: Haga
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
mesh_terms: D000818:Animals; D003072:Cognition Disorders / Q000517:prevention & control*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D008568:Memory; D051379:Mice; D008817:Mice, Mutant Strains; D016501:Neurites / Q000502:physiology*; D010805:Physical Conditioning, Animal; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: C486612:Lztfl1 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors