title: miR-21 functionally interacts with the 3'UTR of chemokine CCL20 and down-regulates CCL20 expression in miR-21 transfected colorectal cancer cells.
abstract: As deregulation of miRNAs and chemokine CCL20 was shown to play a role in colorectal cancer (CRC) pathogenesis, we analyzed the functional interactions of candidate miRNAs with CCL20 mRNA. After target prediction software programs indicated a role for miR-21 in CCL20 regulation, we applied the luciferase reporter assay system to demonstrate that miR-21 functionally interacts with the 3'UTR of CCL20 mRNA and down-regulates CCL20 in miR-21 mimic transfected CRC cell lines (Caco-2, SW480 and SW620). Thus, regulation of CCL20 expression by miR-21 might be a regulatory mechanism involved in progression of CRC.
authors.affiliation: Department of General, Visceral, Vascular and Paediatric Surgery, University of the Saarland, 66421 Homburg/Saar, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Vicinus
authors.forename: Claudia
authors.initials: C
authors.lastname: Rubie
authors.forename: Sabrina K
authors.initials: SK
authors.lastname: Faust
authors.forename: Vilma Oliveira
authors.initials: VO
authors.lastname: Frick
authors.forename: Pirus
authors.initials: P
authors.lastname: Ghadjar
authors.forename: Mathias
authors.initials: M
authors.lastname: Wagner
authors.forename: Stefan
authors.initials: S
authors.lastname: Graeber
authors.forename: Martin K
authors.initials: MK
authors.lastname: Schilling
mesh_terms: D020413:3' Untranslated Regions; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D054418:Chemokine CCL20 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D020413:3' Untranslated Regions; C108355:CCL20 protein, human; D054418:Chemokine CCL20; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger