title: Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA.
abstract: In this study, an esterase, designated EstC23, was isolated from a soil metagenomic library. The protein was amenable to overexpression in Escherichia coli under control of the T7 promoter, resulting in expression of the active, soluble protein that constituted 30% of the total cell protein content. This enzyme showed optimal activity at 40 °C and retained about 50% maximal activity at 5-10 °C. EstC23 showed remarkable stability in up to 50% (v/v) benzene and alkanes (high logP solvents). When incubated for 7 days in the presence of 50% benzene or alkanes, the enzyme maintained its 2-3 fold elevated activity. The purified enzyme also cleaved sterically hindered esters of tertiary alcohols. These results indicate that EstC23 has potential for use in industrial processes.
authors.affiliation: Center for Biomedicine and Health, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310015, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Pei
authors.forename: Pengfei
authors.initials: P
authors.lastname: Du
authors.forename: Xiaopu
authors.initials: X
authors.lastname: Yin
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Xiong
authors.forename: Huili
authors.initials: H
authors.lastname: Wu
authors.forename: Xiuling
authors.initials: X
authors.lastname: Zhou
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D000595:Amino Acid Sequence; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D004950:Esterases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D056186:Metagenomics; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000494:pharmacology*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012997:Solvents / Q000494:pharmacology*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D009930:Organic Chemicals; D012987:Soil; D012997:Solvents; D004247:DNA; D004950:Esterases