title: Consequences of butylated hydroxytoluene in the freezing extender on post-thaw characteristics of stallion spermatozoa in vitro.
abstract: Ejaculates from six pure Spanish stallions were split, and one subsample frozen in a commercial extender supplemented with the lipid soluble antioxidant butylated hydroxytoluene (BHT), while the other subsample served as control. After at least 4 weeks of storage, samples were thawed and post-thaw sperm quality analysed: sperm motility and kinematics using a CASA system, membrane and acrosome integrity and mitochondrial membrane potential using flow cytometry. The outcome of cryopreservation varied significantly among stallions. However, the supplementation with 1 mm BHT had no significant effect on any of the sperm parameters evaluated post-thaw.
authors.affiliation: Laboratory of Equine Reproduction, Veterinary Teaching Hospital, Cáceres, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Morillo-Rodríguez
authors.forename: B
authors.initials: B
authors.lastname: Macías-García
authors.forename: J A
authors.initials: JA
authors.lastname: Tapia
authors.forename: C
authors.initials: C
authors.lastname: Ortega-Ferrusola
authors.forename: F J
authors.initials: FJ
authors.lastname: Peña
mesh_terms: D020101:Acrosome Reaction; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D015925:Cryopreservation; D005434:Flow Cytometry; D005615:Freezing; D006736:Horses; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*
chemical_list: D002084:Butylated Hydroxytoluene