title: Centella asiatica extracts modulate hydrogen peroxide-induced senescence in human dermal fibroblasts.
abstract: Centella asiatica (C. asiatica) is a pharmacological plant in South Asia. It has been demonstrated that C. asiatica extracts containing various pentacyclic triterpenes exert healing effects, especially wound healing and collagen synthesis in skin. However, there are few studies on the effect of C. asiatica extracts on stress-induced premature senescence (SIPS). To determine whether H(2) O(2) -induced senescence is affected by C. asiatica extracts, we performed senescence analysis on cultured human dermal fibroblasts (HDFs). We also analysed whole gene expression level using microarrays and showed that 39 mRNAs are differentially expressed in H(2) O(2) -induced HDFs with and without treatment with C. asiatica extracts. These genes regulate apoptosis, gene silencing, cell growth, transcription, senescence, DNA replication and the spindle checkpoint. Differential expression of FOXM1, E2F2, MCM2, GDF15 and BHLHB2 was confirmed using semi-quantitative PCR. In addition, C. asiatica extracts rescued the H(2) O(2) -induced repression of replication in HDFs. Therefore, the findings presented here suggest that C. asiatica extracts might regulate SIPS by preventing repression of DNA replication and mitosis-related gene expression.
authors.affiliation: Department of Skin Care and Beauty, Osan University, Osan Cosmetology Research Institute, Konkuk University, Seoul, Korea.
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hwa Jun
authors.initials: HJ
authors.lastname: Cha
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Nam
authors.forename: Yeongmin
authors.initials: Y
authors.lastname: Yoon
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Lee
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D028041:Centella; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D020405:Dermis / Q000166:cytology*; D015536:Down-Regulation / Q000235:genetics; D050703:E2F2 Transcription Factor / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D055436:Growth Differentiation Factor 15 / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D064111:Minichromosome Maintenance Complex Component 2; D009363:Neoplasm Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts; D016133:Polymerase Chain Reaction; D014315:Triterpenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D050759:Cyclin-Dependent Kinase Inhibitor p21; D050703:E2F2 Transcription Factor; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D055436:Growth Differentiation Factor 15; D018398:Homeodomain Proteins; D006861:Hydrogen Peroxide; D064111:Minichromosome Maintenance Complex Component 2; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010936:Plant Extracts; D014315:Triterpenes; D016159:Tumor Suppressor Protein p53; C107989:BHLHE40 protein, human; C493956:CDKN1A protein, human; C000614131:CDKN2A protein, human; C526146:Centella asiatica extract; C493761:E2F2 protein, human; C107585:FOXM1 protein, human; C526140:GDF15 protein, human; C084859:MCM2 protein, human