title: NF-κB and CREB are involved in IL-8 production of human neutrophils induced by Trichomonas vaginalis-derived secretory products.
abstract: Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1×10(7) live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-κB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with iκB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-κB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.
authors.affiliation: Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Nam
authors.forename: Deulle
authors.initials: D
authors.lastname: Min
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Kyeong Ah
authors.initials: KA
authors.lastname: Kim
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Lee
authors.forename: Myeong Heon
authors.initials: MH
authors.lastname: Shin
mesh_terms: D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D006805:Human Experimentation; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D009504:Neutrophils / Q000276:immunology*; D010766:Phosphorylation; D014246:Trichomonas vaginalis / Q000276:immunology*
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D016209:Interleukin-8; D016328:NF-kappa B
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