title: An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells.
abstract: We developed an integrated microfluidic culture device to regulate embryonic stem (ES) cell fate. The integrated microfluidic culture device consists of an air control channel and a fluidic channel with 4×4 micropillar arrays. We hypothesized that the microscale posts within the micropillar arrays would enable the control of uniform cell docking and shear stress profiles. We demonstrated that ES cells cultured for 6 days in the integrated microfluidic culture device differentiated into endothelial cells. Therefore, our integrated microfluidic culture device is a potentially powerful tool for directing ES cell fate.
authors.affiliation: Department of Bionano Engineering, Hanyang University, Ansan, Korea.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Ji-eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Edward
authors.initials: E
authors.lastname: Kang
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Bong Geun
authors.initials: BG
authors.lastname: Chung
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology*; D042783:Endothelial Cells / Q000166:cytology*; D004867:Equipment Design; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods