title: Genetic diversity of Babesia bovis in virulent and attenuated strains.
abstract: The aim of this study was to compare the genetic diversity of the single copy Bv80 gene sequences of Babesia bovis in populations of attenuated and virulent parasites. PCR/ RT-PCR followed by cloning and sequence analyses of 4 attenuated and 4 virulent strains were performed. Multiple fragments in the range of 420 to 744 bp were amplified by PCR or RT-PCR. Cloning of the PCR fragments and sequence analyses revealed the presence of mixed subpopulations in either virulent or attenuated parasites with a total of 19 variants with 12 different sequences that differed in number and type of tandem repeats. High levels of intra- and inter-strain diversity of the Bv80 gene, with the presence of mixed populations of parasites were found in both the virulent field isolates and the attenuated vaccine strains. In addition, during the attenuation process, sequence analyses showed changes in the pattern of the parasite subpopulations. Despite high polymorphism found by sequence analyses, the patterns observed and the number of repeats, order, or motifs found could not discriminate between virulent field isolates and attenuated vaccine strains of the parasite.
authors.affiliation: Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel. monica@int.gov.il
authors.forename: M L
authors.initials: ML
authors.lastname: Mazuz
authors.forename: T
authors.initials: T
authors.lastname: Molad
authors.forename: L
authors.initials: L
authors.lastname: Fish
authors.forename: B
authors.initials: B
authors.lastname: Leibovitz
authors.forename: R
authors.initials: R
authors.lastname: Wolkomirsky
authors.forename: L
authors.initials: L
authors.lastname: Fleiderovitz
authors.forename: V
authors.initials: V
authors.lastname: Shkap
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016793:Babesia bovis / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D001404:Babesiosis / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli; D014644:Genetic Variation; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D014613:Vaccines, Attenuated; D014774:Virulence
chemical_list: D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D014613:Vaccines, Attenuated