title: Duplication of partial spinosyn biosynthetic gene cluster in Saccharopolyspora spinosa enhances spinosyn production.
abstract: Spinosyns, the secondary metabolites produced by Saccharopolyspora spinosa, are the active ingredients in a family of insect control agents. Most of the S. spinosa genes involved in spinosyn biosynthesis are found in a contiguous c. 74-kb cluster. To increase the spinosyn production through overexpression of their biosynthetic genes, part of its gene cluster (c. 18 kb) participating in the conversion of the cyclized polyketide to spinosyn was obtained by direct cloning via Red/ET recombination rather than by constructing and screening the genomic library. The resultant plasmid pUCAmT-spn was introduced into S. spinosa CCTCC M206084 from Escherichia coli S17-1 by conjugal transfer. The subsequent single-crossover homologous recombination caused a duplication of the partial gene cluster. Integration of this plasmid enhanced production of spinosyns with a total of 388 (± 25.0) mg L(-1) for spinosyns A and D in the exconjugant S. spinosa trans1 compared with 100 (± 7.7) mg L(-1) in the parental strain. Quantitative real time polymerase chain reaction analysis of three selected genes (spnH, spnI, and spnK) confirmed the positive effect of the overexpression of these genes on the spinosyn production. This study provides a simple avenue for enhancing spinosyn production. The strategies could also be used to improve the yield of other secondary metabolites.
authors.affiliation: College of Life Science, Hunan Normal University, Hunan Provincial Key Laboratory of Microbial Molecular Biology, Changsha, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Luo
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.forename: Yuanwei
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D020869:Gene Expression Profiling; D007306:Insecticides / Q000378:metabolism*; D060847:Metabolic Engineering; D005810:Multigene Family; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D016930:Saccharopolyspora / Q000235:genetics* / Q000378:metabolism*
chemical_list: D007306:Insecticides