title: Molecular characterization of VP4, VP6, VP7 and NSP4 genes of group B rotavirus strains from outbreaks of gastroenteritis.
abstract: OBJECTIVE
  To characterize VP4, VP6, VP7 and NSP4 genes of representative GBR strains (NIV-005625, NIV-04622 and NIV-094456) detected as the major etiologic agent in the outbreaks of gastroenteritis in western India.
  
  
  METHODS
  Fecal specimens collected during the outbreaks of gastroenteritis were processed for RNA isolation, RT-PCR using GBR VP4, VP6, VP7 and NSP4 gene specific primers, nucleotide sequencing of the amplicons and phylogenetic analysis of the sequences.
  
  
  RESULTS
  Phylogenetic analysis of all of the VP4, VP6, VP7 and NSP4 gene sequences revealed clustering of GBR strains in Indian-Bangladeshi lineage of genotype G2 with 95.8%-99.4% nucleotide and 97.3%-100.0% amino acid identities. However, all three strains showed the presence of unique amino acid substitutions in the VP4 protein suggesting alteration in the antigenicity of outbreak strains of GBR. The VP8* and VP5* regions of VP4 proteins showed respectively 0.5%-6.3% and 0.2%-1.1% amino acid divergence from human GBR strains of Indian-Bangladeshi lineage.
  
  
  CONCLUSIONS
  These data confirm the reported variability of VP8* region and suggest the possible role of this region in the perpetuation of GBR infections in the environment. This is the first study to document the phylogenetic relationship of VP4, VP6, VP7 and NSP4 genes of GBR strains detected in the outbreaks of gastroenteritis from India with the GBR strains from other parts of world.
authors.affiliation: Enteric Viruses Group, National Institute of Virology, Pune-411001, India.
authors.forename: Anismrita
authors.initials: A
authors.lastname: Lahon
authors.forename: Shobha D
authors.initials: SD
authors.lastname: Chitambar
mesh_terms: D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000821:virology*; D008040:Genetic Linkage; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*; D014118:Toxins, Biological / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D012367:RNA, Viral; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins