title: RNA targets of wild-type and mutant FET family proteins.
abstract: FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.
authors.affiliation: Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York, USA.
authors.forename: Jessica I
authors.initials: JI
authors.lastname: Hoell
authors.forename: Erik
authors.initials: E
authors.lastname: Larsson
authors.forename: Simon
authors.initials: S
authors.lastname: Runge
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Nusbaum
authors.forename: Sujitha
authors.initials: S
authors.lastname: Duggimpudi
authors.forename: Thalia A
authors.initials: TA
authors.lastname: Farazi
authors.forename: Markus
authors.initials: M
authors.lastname: Hafner
authors.forename: Arndt
authors.initials: A
authors.lastname: Borkhardt
authors.forename: Chris
authors.initials: C
authors.lastname: Sander
authors.forename: Thomas
authors.initials: T
authors.lastname: Tuschl
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009154:Mutation; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D034802:RNA-Binding Protein EWS; D034702:RNA-Binding Protein FUS / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D035381:TATA-Binding Protein Associated Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002148:Calmodulin-Binding Proteins; C500986:EWSR1 protein, human; D012333:RNA, Messenger; D034802:RNA-Binding Protein EWS; D034702:RNA-Binding Protein FUS; D016601:RNA-Binding Proteins; C102041:TAF15 protein, human; D035381:TATA-Binding Protein Associated Factors
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