title: DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools.
abstract: Avidins represent an interesting group of proteins showing high structural similarity and ligand-binding properties but low similarity in primary structure. In this study, we show that it is possible to create functional chimeric proteins from the avidin protein family when applying DNA family shuffling to the genes of the avidin protein family: avidin, avidin related gene 2 and biotin-binding protein A. The novel chimeric proteins were selected by phage display biopanning against biotin, and the selected enriched proteins were characterized, displaying diverse features distinct from the parental genes, including binding to cysteine.
authors.affiliation: Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Niederhauser
authors.forename: Joonas
authors.initials: J
authors.lastname: Siivonen
authors.forename: Juha A
authors.initials: JA
authors.lastname: Määttä
authors.forename: Janne
authors.initials: J
authors.lastname: Jänis
authors.forename: Markku S
authors.initials: MS
authors.lastname: Kulomaa
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001360:Avidin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001710:Biotin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002645:Chickens; D003545:Cysteine; D032621:DNA Shuffling / Q000379:methods*; D019020:Directed Molecular Evolution; D008969:Molecular Sequence Data; D009154:Mutation; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011993:Recombinant Fusion Proteins; D001360:Avidin; D001710:Biotin; D003545:Cysteine