title: Upregulation of low-density lipoprotein receptor after exposure to transforming growth factor-β2 in tenon's capsule fibroblasts from patients with glaucoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the dynamic alteration of a low-density lipoprotein receptor (LDLr) and its binding and uptake of LDL after exposure to transforming growth factor-β(2) (TGF-β(2)) in Tenon's capsule fibroblasts from glaucoma patients.
  
  
  METHODS
  Tenon's capsule fibroblasts obtained from patients who had undergone selective glaucoma surgery were cultured and stimulated with different concentrations (0.1-10 ng/mL) of TGF-β(2) for 1, 3, 5, and 7 days. Expression of LDLr mRNA was detected with relative quantification real-time reverse transcription-polymerase chain reaction, and expression of the corresponding protein was demonstrated with western blot. The extent of binding and uptake of 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI)-labeled LDL by GTFs was also checked.
  
  
  RESULTS
  After exposure to TGF-β(2), LDLr was upregulated at the mRNA and protein levels in a concentration-dependent and/or time-dependent manner with a peak at a concentration of 5 ng/mL TGF-β(2). Binding and uptake of LDL were time dependent, reaching saturation at the first 6 h.
  
  
  CONCLUSIONS
  This study shows that LDLr has a dose- and/or time-effect relationship with TGF-β(2) in Tenon's capsule fibroblasts from glaucoma patients. The results suggest that LDLr may play an important role in wound healing and scar formation in the activated Tenon's capsule fibroblasts from patients with glaucoma.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Li
authors.forename: Weizhong
authors.initials: W
authors.lastname: Zhang
authors.forename: Tingting
authors.initials: T
authors.lastname: Shao
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Qianying
authors.initials: Q
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005901:Glaucoma / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D011973:Receptors, LDL / Q000096:biosynthesis*; D058475:Tenon Capsule / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology*; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects; D055815:Young Adult
chemical_list: C573412:LDLR protein, human; D011973:Receptors, LDL; D053781:Transforming Growth Factor beta2