title: Non-specific defensive factors of the Pacific oyster Crassostrea gigas against infection with Marteilioides chungmuensis: a flow-cytometric study.
abstract: In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Jee Youn
authors.initials: JY
authors.lastname: Hwang
authors.forename: Dong Lim
authors.initials: DL
authors.lastname: Choi
authors.forename: Min Do
authors.initials: MD
authors.lastname: Huh
authors.forename: Young Baek
authors.initials: YB
authors.lastname: Hur
authors.forename: Nam-Sil
authors.initials: NS
authors.lastname: Lee
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.forename: Mun Gyeong
authors.initials: MG
authors.lastname: Kwon
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Choi
authors.forename: Myoung Ae
authors.initials: MA
authors.lastname: Park
mesh_terms: D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D056907:Cercozoa / Q000276:immunology* / Q000472:pathogenicity*; D049896:Crassostrea / Q000276:immunology*; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D006434:Hemocytes / Q000276:immunology; D010587:Phagocytosis
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