title: In vitro and in vivo reduced fitness and virulence in ciprofloxacin-resistant Acinetobacter baumannii.
abstract: Limited data on relative fitness and virulence of antimicrobial-resistant Acinetobacter baumannii are known. We aimed to study the virulence and fitness cost of ciprofloxacin-resistance in A. baumannii (CipR) compared with the susceptible parental wild-type strain (CipS). Human lung epithelial cells were infected with CipS and CipR for 24 h. Competition fitness was monitored in vitro and in vivo in a murine peritoneal sepsis model. We showed that CipR induced less cell death than CipS and CipR growth was slow when in competition with CipS. Altogether, acquisition of ciprofloxacin resistance confers a biological fitness cost and reduces virulence in A. baumannii.
authors.affiliation: Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain. ysmani-ibis@us.es; y_smani@hotmail.com
authors.forename: Y
authors.initials: Y
authors.lastname: Smani
authors.forename: R
authors.initials: R
authors.lastname: López-Rojas
authors.forename: J
authors.initials: J
authors.lastname: Domínguez-Herrera
authors.forename: F
authors.initials: F
authors.lastname: Docobo-Pérez
authors.forename: S
authors.initials: S
authors.lastname: Martí
authors.forename: J
authors.initials: J
authors.lastname: Vila
authors.forename: J
authors.initials: J
authors.lastname: Pachón
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000472:pathogenicity; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D051379:Mice; D008826:Microbial Sensitivity Tests; D010537:Peritoneum / Q000382:microbiology; D018805:Sepsis / Q000382:microbiology; D014774:Virulence
chemical_list: D000890:Anti-Infective Agents; D002939:Ciprofloxacin