title: Cell type-dependent gene regulation by Staufen2 in conjunction with Upf1.
abstract: dendritic mRNA transport machines. Although Stau2 is thought to be involved in the dendritic targeting of several mRNAs in neurons, the mechanism whereby Stau2 regulates these mRNAs is unknown. To elucidate the functions of Stau2, we screened for novel binding partners by affinity purification of GST-tagged Stau2 from 293F cells.
  
  
  RESULTS
  Three RNA helicases, RNA helicase A, Upf1 and Mov10, were identified in Stau2-containing complexes. We focused our studies on Upf1, a key player in nonsense-mediated mRNA decay. Stau2 was found to bind directly to Upf1 in an RNA-independent manner in vitro. Tethering Stau2 to the 3'-untranslated region (UTR) of a reporter gene had little effect on its expression in HeLa cells. In contrast, when the same tethering assay was performed in 293F cells, we observed an increase in reporter protein levels. This upregulation of protein expression by Stau2 turned out to be dependent on Upf1. Moreover, we found that in 293F cells, Stau2 upregulates the reporter mRNA level in an Upf1-independent manner.
  
  
  CONCLUSIONS
  These results indicate that the recruitment of Stau2 alone or in combination with Upf1 differentially affects the fate of mRNAs. Moreover, the results suggest that Stau2-mediated fate determination could be executed in a cell type-specific manner.
authors.affiliation: Department of Frontier Bioscience, Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Miki
authors.forename: Yasunao
authors.initials: Y
authors.lastname: Kamikawa
authors.forename: Sadamu
authors.initials: S
authors.lastname: Kurono
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Jun
authors.initials: J
authors.lastname: Katahira
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D002477:Cells / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D059365:Nonsense Mediated mRNA Decay; D011485:Protein Binding; D020365:RNA Helicases; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013045:Species Specificity; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C492188:Stau2 protein, rat; D015534:Trans-Activators; D020365:RNA Helicases; C101828:UPF1 protein, human
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