title: Fish oil increases atherosclerosis and hepatic steatosis, although decreases serum cholesterol in Wistar rat.
abstract: BACKGROUND
  It is known that fish oil consumption decreases incidence of cardiovascular disease. However, some studies showed that it increases atherosclerosis as it does not get completely metabolized by the liver. The aim of the present study was to investigate the effects of fish oil on aortic atherosclerosis, hepatic steatosis and serum lipids in rats.
  
  
  METHODS
  Twenty pregnant Wistar rats were fed with a fish oil-containing diet or standard diet (containing soy bean oil) during pregnancy and lactation and the pups were weaned onto the same diet. Fasting blood samples, hepatic and aortic specimens were taken from pups on day 70 postnatal. Data were analyzed with SPSS software, using t-test, Mann-Whitney test and Spearman correlation coefficient. Values of p < 0.05 were considered significant.
  
  
  RESULTS
  Medians for fatty streak in aorta of fish oil fed and soy bean oil fed pups were 1.00 and 0.00, respectively, and P value was 0.042. Also, medians for ductular cell hyperplasia of liver in fish oil fed and soy bean oil fed pups were 1.00 and 0.00, respectively, and P value was 0.014. Total cholesterol in pups fed with fish oil was 52.20 mg/dl and in pups fed with soy bean oil was 83.90 mg/dl (p < 0.00) and for low density lipoprotein cholesterol (LDL-C) values were 8.79 mg/dl and 13.16 mg/dl, respectively (p = 0.031).
  
  
  CONCLUSIONS
  According to the results of the present study, a diet which provided 15.9% of energy from fish oil as the only source of dietary fat, induced aortic atherosclerosis as well as hepatic steatosis in Wistar rat, although it decreased total cholesterol and LDL-C.
authors.affiliation: Assistant Professor of Clinical Nutrition, Department of Clinical Nutrition and Dietetics, National Nutrition and Food Technology Research Institute, School of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: M-Shirazi
authors.forename: Fourugh-Azam
authors.initials: FA
authors.lastname: Taleban
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Abadi
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Sabetkasaei
references.citation: Hepatol Int. 2010 Aug 12;4(4):741-8
references.pmid: 21286345
references.citation: J Gastroenterol Hepatol. 2008 Feb;23(2):267-75
references.pmid: 17868330
references.citation: Circ Res. 2009 Nov 20;105(11):1141-8
references.pmid: 19834008
references.citation: Mayo Clin Proc. 2010 Feb;85(2):122-8
references.pmid: 20118387
references.citation: J Nutr Biochem. 2008 Oct;19(10):655-63
references.pmid: 18155507
references.citation: Nutr Res Pract. 2009 Summer;3(2):102-7
references.pmid: 20016709
references.citation: Lab Anim. 1990 Apr;24(2):164-71
references.pmid: 2366514
references.citation: Atherosclerosis. 2006 Jan;184(1):78-85
references.pmid: 15946668
references.citation: J Nutr. 2007 Jul;137(7):1776-82
references.pmid: 17585030
references.citation: J Nutr. 1997 May;127(5 Suppl):838S-841S
references.pmid: 9164249
references.citation: Prog Cardiovasc Dis. 2009 Sep-Oct;52(2):95-114
references.pmid: 19732603
references.citation: Rom J Morphol Embryol. 2010;51(3):509-14
references.pmid: 20809028
references.citation: Atherosclerosis. 1987 Jan;63(1):53-6
references.pmid: 3827970
references.citation: J Pharm Pharmacol. 2005 Nov;57(11):1467-74
references.pmid: 16259780
references.citation: Clin Nutr. 2005 Aug;24(4):492-501
references.pmid: 16054521
references.citation: Lipids. 1990 Sep;25(9):534-40
references.pmid: 2250590
references.citation: Eur J Clin Nutr. 2003 Feb;57(2):218-26
references.pmid: 12571652
references.citation: Atherosclerosis. 2008 Dec;201(2):306-17
references.pmid: 18439610
references.citation: J Nutr Biochem. 2011 Nov;22(11):1009-14
references.pmid: 21190827
references.citation: Food Chem Toxicol. 1998 Aug;36(8):663-72
references.pmid: 9734717
references.citation: Br J Nutr. 1998 Dec;80(6):565-73
references.pmid: 10211055
references.citation: J Am Coll Nutr. 2003 Oct;22(5):388-99
references.pmid: 14559931
references.citation: Can J Physiol Pharmacol. 2009 Dec;87(12):1063-73
references.pmid: 20029543