title: YPR139c/LOA1 encodes a novel lysophosphatidic acid acyltransferase associated with lipid droplets and involved in TAG homeostasis.
abstract: For many years, lipid droplets (LDs) were considered to be an inert store of lipids. However, recent data showed that LDs are dynamic organelles playing an important role in storage and mobilization of neutral lipids. In this paper, we report the characterization of LOA1 (alias VPS66, alias YPR139c), a yeast member of the glycerolipid acyltransferase family. LOA1 mutants show abnormalities in LD morphology. As previously reported, cells lacking LOA1 contain more LDs. Conversely, we showed that overexpression results in fewer LDs. We then compared the lipidome of loa1Δ mutant and wild-type strains. Steady-state metabolic labeling of loa1Δ revealed a significant reduction in triacylglycerol content, while phospholipid (PL) composition remained unchanged. Interestingly, lipidomic analysis indicates that both PLs and glycerolipids are qualitatively affected by the mutation, suggesting that Loa1p is a lysophosphatidic acid acyltransferase (LPA AT) with a preference for oleoyl-CoA. This hypothesis was tested by in vitro assays using both membranes of Escherichia coli cells expressing LOA1 and purified proteins as enzyme sources. Our results from purification of subcellular compartments and proteomic studies show that Loa1p is associated with LD and active in this compartment. Loa1p is therefore a novel LPA AT and plays a role in LD formation.
authors.affiliation: Laboratoire de Biogenèse Membranaire, Université Bordeaux, UMR 5200, F-33000 Bordeaux, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Ayciriex
authors.forename: Marina
authors.initials: M
authors.lastname: Le Guédard
authors.forename: Nadine
authors.initials: N
authors.lastname: Camougrand
authors.forename: Gisèle
authors.initials: G
authors.lastname: Velours
authors.forename: Mario
authors.initials: M
authors.lastname: Schoene
authors.forename: Sebastien
authors.initials: S
authors.lastname: Leone
authors.forename: Valerie
authors.initials: V
authors.lastname: Wattelet-Boyer
authors.forename: Jean-William
authors.initials: JW
authors.lastname: Dupuy
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Schmitter
authors.forename: René
authors.initials: R
authors.lastname: Lessire
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bessoule
authors.forename: Eric
authors.initials: E
authors.lastname: Testet
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D055785:Gene Knockdown Techniques; D006706:Homeostasis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014280:Triglycerides; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase
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