title: Fabrication of an electrochemical platform based on the self-assembly of graphene oxide-multiwall carbon nanotube nanocomposite and horseradish peroxidase: direct electrochemistry and electrocatalysis.
abstract: A novel hybrid nanomaterial (GO-MWNTs) was explored based on the self-assembly of multiwall carbon nanotubes (MWNTs) and graphene oxide (GO). Compared with pristine MWNTs, such a nanocomposite could be well dispersed in aqueous solution and exhibit a negative charge. Driven by the electrostatic interaction, positively charged horseradish peroxidase (HRP) could then be immobilized onto GO-MWNTs at the surface of a glassy carbon (GC) electrode to form a HRP/GO-MWNT/GC electrode under mild conditions. TEM was used to characterize the morphology of the GO-MWNT nanocomposite. UV-vis and FTIR spectra suggested that HRP was immobilized onto the hybrid matrix without denaturation. Furthermore, the immobilized HRP showed enhanced direct electron transfer for the HRP-Fe(III)/Fe(II) redox center. Based on the direct electron transfer of the immobilized HRP, the HRP/GO-MWNT/GC electrode exhibited excellent electrocatalytic behavior to the reduction of H(2)O(2) and NaNO(2), respectively. Therefore, GO-MWNTs could provide a novel and efficient platform for the immobilization and biosensing of redox enzymes, and thus may find wide potential applications in the fabrication of biosensors, biomedical devices, and bioelectronics.
authors.affiliation: Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Shenyang 110036, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shaojun
authors.initials: S
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Pingli
authors.initials: P
authors.lastname: Kang
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
authors.forename: Jingwei
authors.initials: J
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002384:Catalysis; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D015203:Reproducibility of Results; D012977:Sodium Nitrite / Q000032:analysis
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010087:Oxides; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D012977:Sodium Nitrite