title: Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples.
abstract: RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus,                         Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.
authors.affiliation: Plant Virology Group, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia. s.wylie@murdoch.edu.au
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wylie
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Michael G K
authors.initials: MG
authors.lastname: Jones
mesh_terms: D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000821:virology*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral