title: [Human cytomegalovirus glycoprotein B genotypes in congenitally infected neonates].
abstract: OBJECTIVE
  To investigate human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and clinical features in neonates with congenital infections.
  
  
  METHODS
  Urine samples were obtained from 67 neonates with HCMV infection confirmed by polymerase chain reaction (PCR). The gB gene fragment was amplified by nested PCR. HCMV gB genotyping was detected by restriction fragment length polymorphism.
  
  
  RESULTS
  In all these cases, the most prevalent genotype was gBl (50.7%), followed by gB3 (23.9%), gB2 (17.9%), and gBl/gB3 coinfection (7.5%); gB4 was not found. Moreover, gB1 was more prevalent in infants with liver damage (27/37, 73.0%) than in other symptomatic infants without liver damage (13/30, 43.3%; P < 0.05).
  
  
  CONCLUSIONS
  The gBI genotype is the most prevalent in infants with congenital symptomatic HCMV disease, especially in those with liver damage, followed by genotypes gB3, gB2, and gB4.
authors.affiliation: Department of Pediatric Orthopedic, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China. zhangmingang7061@sina.com
authors.forename: Min-gang
authors.initials: MG
authors.lastname: Zhang
authors.forename: Heng-bing
authors.initials: HB
authors.lastname: Wang
authors.forename: Yan-zhou
authors.initials: YZ
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Pan
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000652:urine / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000821:virology*; D008297:Male; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000652:urine
chemical_list: D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus