title: Sensitive electrochemical detection of the hydroxyl radical using enzyme-catalyzed redox cycling.
abstract: Enzyme-catalyzed signal amplification was introduced to the electrochemical detection of the OH radical.  In the presence of phenol as a trapping agent, glucose as a substrate, and pyrroloquinoline quinone-containing glucose dehydrogenase (PQQ-GDH) as a catalyst, the current signal for the trapping adducts (catechol and hydroquinone) produced by the hydroxylation of phenol could be amplified and detected sensitively.  The limit of detection (S/N = 3) for catechol was 8 nM.  The trapping efficiency of phenol was also estimated.
authors.affiliation: International Young Researchers Empowerment Center, Shinshu University, Matsumoto, Nagano, Japan. tatsumi@shinshu-u.ac.jp
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tatsumi
authors.forename: Naoya
authors.initials: N
authors.lastname: Osaku
mesh_terms: D002384:Catalysis; D002396:Catechols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D010084:Oxidation-Reduction; D019800:Phenol / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D002396:Catechols; D017665:Hydroxyl Radical; D019800:Phenol; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D005947:Glucose; C034221:catechol