title: [Identification of Leishmania species in patients and phlebotomines in transmission areas in a region of Peru].
abstract: OBJECTIVE
  To identify the species of Leishmania present in the skin lesions of patients and Lutzomyias living in endemic areas of La Libertad, Peru.
  
  
  METHODS
  Molecular methods based on PCR and RFLP were used, which allowed to have efficient data with small amounts of samples (small specimens), due to their high sensitivity and ease of application in the field work.
  
  
  RESULTS
  The results of PCR of clinical samples of patients and insect vectors showed the presence of Leishmania (V.) peruviana as a major causative agent of andean leishmaniasis transmitted by Lutzomyia peruensis. The presence of Leishmania (V.) guyanensis in Lutzomyia ayacuchensis, was found as well.
  
  
  CONCLUSIONS
  The presence of L. (V.) peruviana and L. (V.) guyanensis in the Andean areas under study was found. These findings remark the need of a wider research about the geographical distribution of L. (V.) guyanensis and clinical features related to the infection in endemic areas of cutaneous leishmaniasis.
authors.affiliation: Universidad Privada Antenor Orrego, La Libertad, Perú.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Córdova
authors.forename: Franklin
authors.initials: F
authors.lastname: Vargas
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hashiguchi
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gómez
mesh_terms: D000818:Animals; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D010568:Peru; D011576:Psychodidae / Q000469:parasitology*; D012867:Skin / Q000469:parasitology*