title: Rapid selection of high-affinity binders using ribosome display.
abstract: Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.
authors.affiliation: Department of Biochemistry, University of Zurich, Zurich, Switzerland.
authors.forename: Birgit
authors.initials: B
authors.lastname: Dreier
authors.forename: Andreas
authors.initials: A
authors.lastname: Plückthun
mesh_terms: D019151:Peptide Library; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D011506:Proteins