title: Human papillomavirus in vaginal intraepithelial neoplasia.
abstract: There are limited data on the prevalence and distribution of human papillomavirus (HPV) genotypes in vaginal intraepithelial neoplasia (VAIN). We sought to clarify this issue in a series of 450 VAIN cases with a confirmed diagnosis between 1990 and 2006. HPV genotyping was performed using paraffin-embedded specimens and polymerase chain reaction (PCR)-based methods. Multiple HPV types were validated by E6 type-specific PCR and direct sequencing. The HPV genotypes of the vaginal and cervical neoplasms were compared for those with incident VAIN and a history of previous/concomitant cervical neoplasms. Ki-67 was performed for supporting diagnosis of VAIN. Of these 450 VAIN cases (median age, 59 years; range, 19-93), two with missing paraffin blocks and 54 with poor DNA quality were excluded. HPV was detected in 273/394 (69.3%) VAIN, and multiple infections were found in 17.9% of HPV-positive samples. The leading types were HPV16 (35.5%), HPV58 (9.9%), HPV52 (9.9%), HPV39 (8.4%), HPV33 (7.3%) and HPV53 (7.0%). Among the 156 cases with a history of previous cervical neoplasia, 29.0% had concordant HPV genotypes, while synchronous VAIN samples (n = 49) were more likely to harbor concordant genotypes (58.7%) with the concomitant cervical neoplasm (p = 0.0003). Whether those HPV types in the incident VAIN lesions had existed in the vaginal epithelium at the time of the previous cervical neoplasia or a new acquisition needs to be clarified in prospective follow-up studies.
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Angel
authors.initials: A
authors.lastname: Chao
authors.forename: Tse-Ching
authors.initials: TC
authors.lastname: Chen
authors.forename: Chuen
authors.initials: C
authors.lastname: Hsueh
authors.forename: Chu-Chun
authors.initials: CC
authors.lastname: Huang
authors.forename: Jung-Erh
authors.initials: JE
authors.lastname: Yang
authors.forename: Swei
authors.initials: S
authors.lastname: Hsueh
authors.forename: Huei-Jean
authors.initials: HJ
authors.lastname: Huang
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Lin
authors.forename: Yun-Hsin
authors.initials: YH
authors.lastname: Tang
authors.forename: Jui-Der
authors.initials: JD
authors.lastname: Liou
authors.forename: Chee-Jen
authors.initials: CJ
authors.lastname: Chang
authors.forename: Hung-Hsueh
authors.initials: HH
authors.lastname: Chou
authors.forename: Chyong-Huey
authors.initials: CH
authors.lastname: Lai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D002278:Carcinoma in Situ / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D014625:Vaginal Neoplasms / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D019394:Ki-67 Antigen