title: Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole.
abstract: Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography demonstrated that DC and CTC were completely eliminated after 15 min, while TC and CTC were eliminated after 1 h. This system also resulted in a complete loss of inhibition of growth of Escherichia coli and Bacillus subtilis and the green alga Pseudokirchneriella subcapitata with decreasing tetracycline antibiotic concentration. These results suggest that the laccase-HBT system is effective in eliminating tetracycline antibiotics and removing their ecotoxicity.
authors.affiliation: Faculty of Agriculture, Shizuoka University, Ohya 836, Surugaku, Shizuoka 422-8529, Japan.
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Suda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hata
authors.forename: Shingo
authors.initials: S
authors.lastname: Kawai
authors.forename: Hideo
authors.initials: H
authors.lastname: Okamura
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D014230:Triazoles; D008031:Lignin; C011852:1-hydroxybenzotriazole; D042845:Laccase; D013752:Tetracycline