title: Clinical significance of plasma CD146 and P-selectin in patients with type 2 diabetic nephropathy.
abstract: OBJECTIVE
  To investigate the levels of plasma CD146 and P-selectin in patients with type 2 diabetic nephropathy at different stages.
  
  
  METHODS
  A total of 80 patients with type 2 diabetes mellitus were enrolled in the present study. According to 24h urinary albumin excretion ratio and renal function, they were further divided into group of diabetes without microalbuminuria (DN0, n=20), microalbuminuria group (DN1, n=20), macroalbuminuria group (DN2, n=20) and renal insufficiency group (DN3, n=20). Another 20 healthy subjects were enrolled as control group (non-DM). Plasma CD146 and P-selectin were measured by ELISA.
  
  
  RESULTS
  Plasma CD146 and P-selectin were significantly increased in patients with type 2 diabetes with microalbuminuria (DN1) compared with health control (CD146: 415.3±29.0 vs. 243.5±14.7 ng/ml, P<0.05; P-selectin: 66.8±3.4 vs. 45.3±2.7 ng/ml, P<0.001). With the development of diabetic nephropathy, both plasma CD146 and P-selectin level progressively rise, with the highest levels in patients with significant renal insufficiency (DN3: 515.9±36.9 and 81.5±5.1 ng/ml respectively, P<0.001). Moreover, the increase in CD146 is positively co-related to the rise of P-selectin in patients with type 2 diabetes.
  
  
  CONCLUSIONS
  Expression of CD146 and P-selectin in patients with type 2 diabetes is elevated, and they are positively correlated with severity of diabetic nephropathy.
authors.affiliation: Department of Nephrology and Rheumatology, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Xing
authors.forename: Niansong
authors.initials: N
authors.lastname: Wang
authors.forename: Limei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D051929:CD146 Antigen / Q000097:blood; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications*; D003928:Diabetic Nephropathies / Q000097:blood* / Q000150:complications*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood*
chemical_list: D051929:CD146 Antigen; C497902:MCAM protein, human; D019007:P-Selectin; C546937:SELP protein, human; D002097:C-Reactive Protein