title: Contact-dependent T cell activation and T cell stopping require talin1.
abstract: T cell-APC contact initiates T cell activation and is maintained by the integrin LFA-1. Talin1, an LFA-1 regulator, localizes to the immune synapse (IS) with unknown roles in T cell activation. In this study, we show that talin1-deficient T cells have defects in contact-dependent T cell stopping and proliferation. Although talin1-deficient T cells did not form stable interactions with APCs, transient contacts were sufficient to induce signaling. In contrast to prior models, LFA-1 polarized to T cell-APC contacts in talin1-deficient T cells, but vinculin and F-actin polarization at the IS was impaired. These results indicate that T cell proliferation requires sustained, talin1-mediated T cell-APC interactions and that talin1 is necessary for F-actin polarization and the stability of the IS.
authors.affiliation: Program in Cellular and Molecular Biology, University of Wisconsin, Madison, WI 53705, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Wernimont
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Wiemer
authors.forename: David A
authors.initials: DA
authors.lastname: Bennin
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Monkley
authors.forename: Thomas
authors.initials: T
authors.lastname: Ludwig
authors.forename: David R
authors.initials: DR
authors.lastname: Critchley
authors.forename: Anna
authors.initials: A
authors.lastname: Huttenlocher
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002450:Cell Communication / Q000235:genetics / Q000276:immunology*; D016764:Cell Polarity / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D054992:Immunological Synapses / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D016608:Talin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D016169:Lymphocyte Function-Associated Antigen-1; D016608:Talin; C496077:Tln1 protein, mouse; D016596:Vinculin
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