title: Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.
abstract: Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Cao
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Itoi
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Honda
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Takeshi
authors.initials: T
authors.lastname: Omasa
mesh_terms: D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D022202:Chromosomes, Artificial, Bacterial; D006224:Cricetinae; D003412:Cricetulus; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*