title: Patterned cell adhesion associated with tissue deformations during dorsal closure in Drosophila.
abstract: Cell shape changes within epithelia require the regulation of adhesive molecules that maintain tissue integrity. How remodelling of cell contacts is achieved while tissue integrity is maintained remains a fundamental question in morphogenesis. Dorsal Closure is a good system to study the dynamics of DE-Cadherin during morphogenesis. It relies on concerted cell shape changes of two epithelial sheets: amnioserosa cell contraction and epidermal cell elongation. To investigate the modulation of DE-Cadherin we performed antibody uptake experiments in live embryos during Dorsal Closure. We found that some antibodies access certain epitopes of the extracellular domain of native DE-Cadherin only in the amnioserosa and epidermal cells attached to the amnioserosa, which has never been observed in fixed DE-Cadherin in Drosophila embryos. These differences correlate with the different cell behaviour of these regions and therefore we suggest that DE-Cadherin exists in different forms that confer different adhesive strengths. We propose this to be a widespread mechanism for the differential modulation of adhesion during morphogenesis.
authors.affiliation: Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Mateus
authors.forename: Alfonso
authors.initials: A
authors.lastname: Martinez Arias
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000276:immunology; D015820:Cadherins / Q000276:immunology / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D029721:Drosophila Proteins / Q000276:immunology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000276:immunology / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D009024:Morphogenesis / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D015820:Cadherins; D029721:Drosophila Proteins; C501889:shg protein, Drosophila
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