title: DNA binding, DNA cleavage and cytotoxicity studies of a new water soluble copper(II) complex: the effect of ligand shape on the mode of binding.
abstract: The interaction of native calf thymus DNA (CT-DNA) with [Cu(ph(2)phen)(phen-dione)Cl]Cl was studied at physiological pH by spectrophotometric, spectrofluorometric, circular dichroism, and viscometric techniques. Considerable hypochromicity and red shift are observed in the UV absorption band of the Cu complex. Binding constants (K(b)) of DNA with the complex were calculated at different temperatures. Thermodynamic parameters, enthalpy and entropy changes were calculated according to Van't Hoff equation, which indicated that reaction is predominantly enthalpically driven. All these results indicate that Cu(II) complex interacts with CT-DNA via intercalative mode. Also, this new complex induced cleavage in pUC18 plasmid DNA as indicated in gel electrophoresis and showed excellent antitumor activity against K562 (human chronic myeloid leukemia) and human T lymphocyte carcinoma-Jurkat cell lines.
authors.affiliation: Department of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Faculty of Science, Razi University, P.O. Box: 67149, Kermanshah, Iran. kashanian s@yahoo.com
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Khodaei
authors.forename: Hamideh
authors.initials: H
authors.lastname: Roshanfekr
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.forename: Ghobad
authors.initials: G
authors.lastname: Mansouri
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000378:metabolism* / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D055664:Electrochemical Techniques; D006801:Humans; D007454:Iodides / Q000737:chemistry; D019169:Jurkat Cells; D020014:K562 Cells; D007700:Kinetics; D008024:Ligands; D009994:Osmolar Concentration; D010957:Plasmids / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014783:Viscosity / Q000187:drug effects; D014867:Water / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007454:Iodides; D008024:Ligands; D014867:Water; D012965:Sodium Chloride; D003300:Copper; D004247:DNA; C427203:calf thymus DNA