title: [Establishment of cellular immunity of enhanced hepatitis B vaccine].
abstract: OBJECTIVE
  To establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary.
  
  
  METHODS
  Immunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-gamma spots forming cells (SFC) of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared.
  
  
  RESULTS
  IFN-gamma SFC was higher in 5microg dose than in 2microg dose after immunization with enhanced hepatitis B vaccine and IFN-gamma SFC was declined after immunization 3 weeks ago. IFN-gamma SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-gamma SFC was higher in enhanced hepatitis B vaccine.
  
  
  CONCLUSIONS
  Established the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.
authors.affiliation: National Vaccine & Serum Institute, Beijing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Ji-lai
authors.initials: JL
authors.lastname: Li
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D005260:Female; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007371:Interferon-gamma