title: A determination of potential α-glucosidase inhibitors from Azuki Beans (Vigna angularis).
abstract: A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH(2)Cl(2), EtOAc and n-BuOH to afford four fractions: CH(2)Cl(2)-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC(50) values of 0.4 mg·mL(-1) and 4.8 mg·mL(-1), respectively. This is the first study of the active compositions of azuki beans against α-glucosidase.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, South Xueyuan Road, Haidian District No.80, Beijing 100081, China; E-Mails:  yaoyang@caas.net.cn  (Y.Y.);  chengxz@caas.net.cn  (X.C.); wanglx@caas.net.cn  (L.W.);  wangsh@caas.net.cn  (S.W.);
authors.forename: Yang
authors.initials: Y
authors.lastname: Yao
authors.forename: Xuzhen
authors.initials: X
authors.lastname: Cheng
authors.forename: Lixia
authors.initials: L
authors.lastname: Wang
authors.forename: Suhua
authors.initials: S
authors.lastname: Wang
authors.forename: Guixing
authors.initials: G
authors.lastname: Ren
mesh_terms: D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D005959:Glucosidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000431:Ethanol; D047310:Apigenin; C032731:vitexin; D005959:Glucosidases; C049772:isovitexin
references.citation: Naturwissenschaften. 1977 Oct;64(10):536-7
references.pmid: 927538
references.citation: J Nat Prod. 1998 Nov;61(11):1413-5
references.pmid: 9834167
references.citation: J Agric Food Chem. 2006 May 31;54(11):3807-14
references.pmid: 16719500
references.citation: Diabetes Res Clin Pract. 1998 Jul;40 Suppl:S27-8
references.pmid: 9740499
references.citation: Drugs. 2005;65(3):385-411
references.pmid: 15669880
references.citation: J Agric Food Chem. 2008 Oct 8;56(19):8869-73
references.pmid: 18767859
references.citation: J Agric Food Chem. 2009 Dec 23;57(24):11463-8
references.pmid: 19938837
references.citation: Biosci Biotechnol Biochem. 2004 Dec;68(12):2421-6
references.pmid: 15618610
references.citation: J Agric Food Chem. 2010 Jan 27;58(2):770-4
references.pmid: 19904935