title: Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C=C double bond migration useful for conjugated fatty acid synthesis.
abstract: Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of cis-9, trans-11-CLA, in lactic acid bacteria.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Kyoto 606-8502, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kishino
authors.forename: Si-Bum
authors.initials: SB
authors.lastname: Park
authors.forename: Michiki
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002384:Catalysis; D003001:Cloning, Molecular; D004798:Enzymes / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D044243:Linoleic Acids, Conjugated; C503589:cis-9, trans-11-conjugated linoleic acid