title: Pseudogenes of rat VDAC1: 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues.
abstract: BLAST analysis of the rat genome revealed the presence of 16 pseudogenes of isoform 1 of the mitochondrial voltage-dependent anion channel (VDAC1). Based on their structural characterization, it was concluded that these pseudogenes were formed by integration of VDAC1 cDNA into the genome, and subsequent rearrangements/mutations. By RT-PCR analysis using carefully designed primers that could not amplify the cDNA of genuine VDAC1, 8 of these 16 pseudogenes showed slight expression in certain tissues, but none of them seemed to encode a functional protein.
authors.affiliation: Institute for Genome Research, University of Tokushima, Kuramoto-cho-3, Tokushima, 770-8503, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ido
authors.forename: Takenori
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Eriko
authors.initials: E
authors.lastname: Obana
authors.forename: Kazuto
authors.initials: K
authors.lastname: Ohkura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D016678:Genome; D009928:Organ Specificity; D011544:Pseudogenes; D051381:Rats / Q000235:genetics* / Q000378:metabolism; D050995:Voltage-Dependent Anion Channel 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C494764:Vdac1 protein, rat; D050995:Voltage-Dependent Anion Channel 1
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