title: Soil bacterial communities of a calcium-supplemented and a reference watershed at the Hubbard Brook Experimental Forest (HBEF), New Hampshire, USA.
abstract: Soil Ca depletion because of acidic deposition-related soil chemistry changes has led to the decline of forest productivity and carbon sequestration in the northeastern USA. In 1999, acidic watershed (WS) 1 at the Hubbard Brook Experimental Forest (HBEF), NH, USA was amended with Ca silicate to restore soil Ca pools. In 2006, soil samples were collected from the Ca-amended (WS1) and reference watershed (WS3) for comparison of bacterial community composition between the two watersheds. The sites were about 125 m apart and were known to have similar stream chemistry and tree populations before Ca amendment. Ca-amended soil had higher Ca and P, and lower Al and acidity as compared with the reference soils. Analysis of bacterial populations by PhyloChip revealed that the bacterial community structure in the Ca-amended and the reference soils was significantly different and that the differences were more pronounced in the mineral soils. Overall, the relative abundance of 300 taxa was significantly affected. Numbers of detectable taxa in families such as Acidobacteriaceae, Comamonadaceae, and Pseudomonadaceae were lower in the Ca-amended soils, while Flavobacteriaceae and Geobacteraceae were higher. The other functionally important groups, e.g. ammonia-oxidizing Nitrosomonadaceae, had lower numbers of taxa in the Ca-amended organic soil but higher in the mineral soil.
authors.affiliation: Department of Biological Sciences, University of New Hampshire, Durham, NH, USA.
authors.forename: Ganapathi
authors.initials: G
authors.lastname: Sridevi
authors.forename: Rakesh
authors.initials: R
authors.lastname: Minocha
authors.forename: Swathi A
authors.initials: SA
authors.lastname: Turlapati
authors.forename: Katherine C
authors.initials: KC
authors.lastname: Goldfarb
authors.forename: Eoin L
authors.initials: EL
authors.lastname: Brodie
authors.forename: Louis S
authors.initials: LS
authors.lastname: Tisa
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Minocha
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D001483:Base Sequence; D044822:Biodiversity; D002118:Calcium / Q000032:analysis*; D058645:Denaturing Gradient Gel Electrophoresis; D017753:Ecosystem; D052918:Environmental Restoration and Remediation / Q000379:methods*; D008969:Molecular Sequence Data; D009513:New Hampshire; D045483:Rivers / Q000737:chemistry; D017640:Silicates / Q000032:analysis; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014197:Trees
chemical_list: D017640:Silicates; D012987:Soil; D002118:Calcium