title: A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices.
abstract: Fraction collection of selected components from a complex mixture plays a critical role in biomedical research, environmental analysis, and biotechnology. Here, we introduce a novel electrophoretic chip device based on a signal processing theorem that allows simultaneous space sampling for fractionation of ssDNA target fragments. Ten parallel extraction channels, which covered 1.5-mm-long sampling ranges, were used to facilitate the capturing of fast-moving fragments. Furthermore, the space sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Fragments of 180, 181, and 182 bases were simultaneously collected, and then the recovered DNA was PCR amplified and assessed by CE analysis. The 181-base target was shown to be isolated in a 70-mm-long separation length within 10 min, in contrast to the >50 min required for the 300-mm-long separation channel in our previous study. This method provides effective combination of time and space, which is a breakthrough in the traditional concept of fraction collection on a chip.
authors.affiliation: Research Institute for Electronic Science, Hokkaido University, Kita-ku, Sapporo, Japan.
authors.forename: Zheyu
authors.initials: Z
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Misato
authors.initials: M
authors.lastname: Sunayama
authors.forename: Ryoko
authors.initials: R
authors.lastname: Araki
authors.forename: Kosei
authors.initials: K
authors.lastname: Ueno
authors.forename: Masumi
authors.initials: M
authors.lastname: Abe
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Misawa
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D016133:Polymerase Chain Reaction; D012815:Signal Processing, Computer-Assisted; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D004247:DNA