title: Nuclei of Tsuga canadensis: role of flavanols in chromatin organization.
abstract: Needle primordia of Tsuga canadensis (hemlock) arising from flank meristems of a shoot apex, form cell lineages consisting of four or eight cells. Within a recently established lineage there is striking uniformity in the pattern of nuclear flavanols. This fact points to an identical transcriptional expression of these flavanols during cell cycling. However two lineages, even if located close together within the same meristem, can be very different in the expression of both cell shape and nuclear flavanol pattern, indicating that epigenetic positional signals are operating in a collective specification of cell lineage development. There is a wide range of nuclear flavanol patterning from a mosaic-like distribution in an activated cell type to a homogenous appearance in silenced cell types. Single cells deriving from lineages are desynchronized because they underlie a signaling network at a higher tissue level which results in stronger epigenetic modifications of their nuclear flavanols. As an extreme case of epigenetic modulation, transient drought conditions caused a drastic reduction of nuclear flavanols. Upon treatment with sucrose or cytokinin, these nuclear flavanols could be fully restored. Analytical determination of the flavanols revealed 3.4 mg/g DW for newly sprouting needles and 19.6 mg/g DW for anthers during meiosis. The roughly 6-fold difference in flavanols is apparently a reflection of the highly diverging organogenetic processes. Collectively, the studies provide strong evidence for combinatorial interplay between cell fate and nuclear flavanols.
authors.affiliation: Unit Fruit Science, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Dürnast 2, D-85354 Freising, Germany; E-Mail:  obstbau@wzw.tum.de.
authors.forename: Walter
authors.initials: W
authors.lastname: Feucht
authors.forename: Markus
authors.initials: M
authors.lastname: Schmid
authors.forename: Dieter
authors.initials: D
authors.lastname: Treutter
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D003583:Cytokinins / Q000494:pharmacology; D044948:Flavonols / Q000494:pharmacology*; D008540:Meiosis / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology; D028225:Tsuga
chemical_list: D002843:Chromatin; D003583:Cytokinins; D044948:Flavonols; D013395:Sucrose
references.citation: Plant Cell. 2002;14 Suppl:S303-25
references.pmid: 12045285
references.citation: Trends Plant Sci. 2008 Mar;13(3):137-44
references.pmid: 18314376
references.citation: J Exp Bot. 2006;57(1):113-24
references.pmid: 16291799
references.citation: Trends Cell Biol. 2005 Jul;15(7):349-55
references.pmid: 15946846
references.citation: Curr Opin Plant Biol. 2008 Feb;11(1):16-22
references.pmid: 18409210
references.citation: Plant J. 2003 Feb;33(3):471-80
references.pmid: 12581305
references.citation: Curr Opin Plant Biol. 2009 Oct;12(5):628-36
references.pmid: 19700365
references.citation: Cell. 2009 Mar 20;136(6):1005-16
references.pmid: 19303845
references.citation: Plant Biol (Stuttg). 2005 Nov;7(6):581-91
references.pmid: 16388461
references.citation: Curr Opin Plant Biol. 2009 Oct;12(5):599-605
references.pmid: 19700366
references.citation: Curr Opin Plant Biol. 2005 Oct;8(5):518-25
references.pmid: 16054432
references.citation: Cell. 2007 Feb 23;128(4):787-800
references.pmid: 17320514
references.citation: Plant Cell Rep. 2004 Jan;22(6):430-6
references.pmid: 14595514
references.citation: Plant Cell. 2000 May;12(5):617-36
references.pmid: 10810139
references.citation: J Biol Chem. 1997 Mar 7;272(10):6799-804
references.pmid: 9045714
references.citation: Cell. 2007 Feb 23;128(4):707-19
references.pmid: 17320508
references.citation: Cell. 2007 Feb 23;128(4):693-705
references.pmid: 17320507
references.citation: Tree Physiol. 2008 Dec;28(12):1783-91
references.pmid: 19193561
references.citation: Biochem Biophys Res Commun. 2005 Jul 22;333(1):101-9
references.pmid: 15950188
references.citation: Development. 2003 Aug;130(16):3735-45
references.pmid: 12835390
references.citation: Biol Chem. 2003 Jul;384(7):997-1006
references.pmid: 12956416
references.citation: Cell. 2002 Feb 22;108(4):489-500
references.pmid: 11909520
references.citation: Science. 2001 Feb 2;291(5505):843-7
references.pmid: 11225636
references.citation: Biochimie. 2005 Jul;87(7):625-35
references.pmid: 15989979
references.citation: Trends Cell Biol. 2009 May;19(5):207-17
references.pmid: 19342239
references.citation: Plant Cell. 2000 Nov;12(11):2087-100
references.pmid: 11090211
references.citation: J Histochem Cytochem. 1981 Aug;29(8):959-68
references.pmid: 6168681
references.citation: Nature. 2000 Jul 6;406(6791):98-101
references.pmid: 10894550
references.citation: Plant J. 2002 Oct;32(2):221-31
references.pmid: 12383087
references.citation: Protoplasma. 1974;79(1):127-43
references.pmid: 4844712
references.citation: Curr Opin Plant Biol. 2005 Dec;8(6):600-5
references.pmid: 16182599
references.citation: Curr Opin Plant Biol. 2005 Feb;8(1):93-102
references.pmid: 15653406
references.citation: Science. 2003 Aug 8;301(5634):798-802
references.pmid: 12907790
references.citation: Cell. 2002 Feb 22;108(4):513-21
references.pmid: 11909522
references.citation: Plant Physiol. 2001 Jan;125(1):112-4
references.pmid: 11154310
references.citation: Science. 2001 Dec 21;294(5551):2539-42
references.pmid: 11598266
references.citation: J Cell Sci. 2000 Sep;113 ( Pt 18):3217-26
references.pmid: 10954420
references.citation: Stain Technol. 1985 Sep;60(5):285-94
references.pmid: 2412317
references.citation: Theor Appl Genet. 2010 May;120(7):1315-33
references.pmid: 20058146
references.citation: Plant Cell. 2002;14 Suppl:S47-59
references.pmid: 12045269
references.citation: Nature. 2002 Feb 14;415(6873):751-4
references.pmid: 11845197