title: Induction of the Escherichia coli cysK gene by genetic and environmental factors.
abstract: Cysteine synthase A encoded by cysK catalyzes the synthesis of cysteine from O-acetylserine. Expression of cysK in Escherichia coli is under the control of CysB, a LysR family transcription factor. Herein we showed that the expression of cysK is regulated by several genetic and environmental factors in addition to CysB: two genetic factors, OmpR and CysE, and lithium. Based on the findings, we constructed the high-level expression system of cysK.
authors.affiliation: Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Koganei, Tokyo, Japan. kanyamam@hosei.ac.jp
authors.forename: Kaneyoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
authors.forename: Gen
authors.initials: G
authors.lastname: Nonaka
authors.forename: Hisao
authors.initials: H
authors.lastname: Ito
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D003547:Cysteine Synthase / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008094:Lithium / Q000378:metabolism; D051050:Serine O-Acetyltransferase / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; D008094:Lithium; C070785:CysE protein, E coli; D051050:Serine O-Acetyltransferase; D003547:Cysteine Synthase