title: Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements.
abstract: Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the -35, -15, -10 and the +1 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein.
authors.affiliation: Institute of Genetics, University of Bayreuth, D-95445 Bayreuth, Germany.
authors.forename: Trang Thi Phuong
authors.initials: TT
authors.lastname: Phan
authors.forename: Hoang Duc
authors.initials: HD
authors.lastname: Nguyen
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schumann
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D015152:Blotting, Northern; D018833:Chaperonins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D007544:Isopropyl Thiogalactoside; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D011994:Recombinant Proteins; D007544:Isopropyl Thiogalactoside; D018833:Chaperonins