title: [Proteolysis of semax analogues with different N-terminal amino acids by aminopeptidases].
abstract: Proteolysis of semax (Met-Glu-His-Phe-Pro-Gly-Pro, Sem) and its analogues ([Ala1]Sem, [Gly1]Sem, [Thr1]Sem, [Trp1]Sem) that are differ from semax in substitution of N-terminal Met residue were studied. It is shown that such replacement changes the rate of peptides degradation by N-aminopeptidases (EC 3.4.11.2, Sigma, Type VI, 9.2 units. Akt. / mg). [Ala1]Sem, [Gly1]Sem and [Thr1]Sem semax analogues proved to be more stable to proteolysis than semax (Sem), and their initial product of proteolysis is His-Phe-Pro-Gly-Pro (Sem-5). For triptophan analogue both Glu-His-Phe-Pro-Gly-Pro (Sem-6) and Sem-5 product are formed in similar quantities. It is found that all investigated analogues can be used as inhibitors in Sem proteolysis.
authors.forename: K V
authors.initials: KV
authors.lastname: Shevchenko
authors.forename: T V
authors.initials: TV
authors.lastname: V'iunova
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Nagaev
authors.forename: L A
authors.initials: LA
authors.lastname: Andreeva
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Alfeeva
authors.forename: N F
authors.initials: NF
authors.lastname: Miasoedov
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D018826:CD13 Antigens / Q000737:chemistry / Q000378:metabolism*; D008566:Membranes / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D059748:Proteolysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D010455:Peptides; C048487:ACTH (4-7), Pro-Gly-Pro-; D000324:Adrenocorticotropic Hormone; D018826:CD13 Antigens