title: Superresolution optical fluctuation imaging (SOFI).
abstract: Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation -imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.
authors.affiliation: Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA. thomasd@chem.ucla.edu
authors.forename: Thomas
authors.initials: T
authors.lastname: Dertinger
authors.forename: Ryan
authors.initials: R
authors.lastname: Colyer
authors.forename: Robert
authors.initials: R
authors.lastname: Vogel
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann
authors.forename: Markus
authors.initials: M
authors.lastname: Sauer
authors.forename: Jörg
authors.initials: J
authors.lastname: Enderlein
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003584:Cytological Techniques / Q000379:methods*; D005347:Fibroblasts / Q000648:ultrastructure; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*