title: Reduction of Seed Dormancy in Echinacea pallida (Nutt.) Nutt. by In-dark Seed Selection and Breeding.
abstract: Strong seed dormancy has been an obstacle for field production of Echinacea species. Previous research on overcoming Echinacea seed dormancy has been extensive and focused on treatment methods, which involve time and expense, and are incompatible with organic production if synthetic chemicals are used. We have attempted to genetically reduce seed dormancy through selection and breeding in Echinacea, by using E. pallida as a model species. Three accessions were used in this study. Nine parent plants of each accession selected from early, in-dark germinated seeds (in-dark plants) or from late, in-light seeds (in-light plants) were planted and grouped by accession and germination treatment method for seed production through a polycross method. Germination tests indicated that these in-dark plants produced seed (in-dark seed) with significantly reduced seed dormancy when tested under light or dark conditions in comparison to the seed of the in-light plants (in-light seed). Among the three accessions, the in-dark seed germinated at much higher rates than did the in-light seed, more than 2× at 25°C under light and up to an 83× increase in darkness, and up to an 8× increase over the corresponding parental seed lots under comparable germination conditions. In addition to these increases in germination, the in-dark seed showed early and synchronized germination as compared to the in-light seed. Since these results were achieved through only one cycle of selection and breeding, they strongly suggest that we have developed a very effective method for modifying seed dormancy in Echinacea.
authors.affiliation: USDA-ARS, North Central Regional Plant Introduction Station, Iowa State University, Ames, IA 50011-1170.
authors.forename: Luping
authors.initials: L
authors.lastname: Qu
authors.forename: Mark P
authors.initials: MP
authors.lastname: Widrlechner
references.citation: Ann Intern Med. 2010 Dec 21;153(12):769-77
references.pmid: 21173411
references.citation: HortScience. 2004 Aug;39(5):1101-1103
references.pmid: 17075609
references.citation: J Exp Bot. 2011 Jan;62(2):627-40
references.pmid: 20978143
references.citation: Plant Cell. 1997 Jul;9(7):1055-1066
references.pmid: 12237375
references.citation: Planta Med. 2010 Feb;76(3):265-72
references.pmid: 19790030
references.citation: Phytomedicine. 2009 Jun;16(6-7):669-78
references.pmid: 19303756
references.citation: New Phytol. 2006;171(3):501-23
references.pmid: 16866955
references.citation: HortScience. 2005 Oct;40(6):1843-1845
references.pmid: 16429595
references.citation: J Ethnopharmacol. 2007 Mar 1;110(1):148-53
references.pmid: 17052874
references.citation: Lancet Infect Dis. 2007 Jul;7(7):473-80
references.pmid: 17597571
references.citation: HortScience. 2005 Oct;40(6):1751-1754
references.pmid: 17160141
references.citation: Holist Nurs Pract. 2010 Mar-Apr;24(2):107-9
references.pmid: 20186021
references.citation: Annu Rev Plant Biol. 2008;59:387-415
references.pmid: 18257711
references.citation: Am J Bot. 2008 Jun;95(6):756-65
references.pmid: 21632401
references.citation: Planta Med. 2008 May;74(6):633-7
references.pmid: 18186015