title: Comparative genomics of Vibrio cholerae from Haiti, Asia, and Africa.
abstract: Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 nonmatched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
authors.affiliation: Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: Aleisha R
authors.initials: AR
authors.lastname: Reimer
authors.forename: Gary
authors.initials: G
authors.lastname: Van Domselaar
authors.forename: Steven
authors.initials: S
authors.lastname: Stroika
authors.forename: Matthew
authors.initials: M
authors.lastname: Walker
authors.forename: Heather
authors.initials: H
authors.lastname: Kent
authors.forename: Cheryl
authors.initials: C
authors.lastname: Tarr
authors.forename: Deborah
authors.initials: D
authors.lastname: Talkington
authors.forename: Lori
authors.initials: L
authors.lastname: Rowe
authors.forename: Melissa
authors.initials: M
authors.lastname: Olsen-Rasmussen
authors.forename: Michael
authors.initials: M
authors.lastname: Frace
authors.forename: Scott
authors.initials: S
authors.lastname: Sammons
authors.forename: Georges Anicet
authors.initials: GA
authors.lastname: Dahourou
authors.forename: Jacques
authors.initials: J
authors.lastname: Boncy
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Smith
authors.forename: Philip
authors.initials: P
authors.lastname: Mabon
authors.forename: Aaron
authors.initials: A
authors.lastname: Petkau
authors.forename: Morag
authors.initials: M
authors.lastname: Graham
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Gilmour
authors.forename: Peter
authors.initials: P
authors.lastname: Gerner-Smidt
mesh_terms: D000349:Africa / Q000453:epidemiology; D000483:Alleles; D001208:Asia / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D002771:Cholera / Q000453:epidemiology; D002772:Cholera Toxin / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D023061:Gene Order; D016680:Genome, Bacterial; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics; D010802:Phylogeny; D039002:Prophages; D017386:Sequence Homology, Amino Acid; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D002772:Cholera Toxin
references.citation: Bioinformatics. 2007 Mar 15;23(6):673-9
references.pmid: 17237039
references.citation: PLoS One. 2008;3(12):e4053
references.pmid: 19115014
references.citation: J Infect. 2008 Sep;57(3):280-1
references.pmid: 18657323
references.citation: Emerg Infect Dis. 2010 Nov;16(11):1804-5
references.pmid: 21029554
references.citation: J Bacteriol. 2010 Jul;192(13):3524-33
references.pmid: 20348258
references.citation: J Clin Microbiol. 2009 May;47(5):1568-71
references.pmid: 19297603
references.citation: Jpn J Med Sci Biol. 1970 Feb;23(1):13-20
references.pmid: 5310994
references.citation: Trends Microbiol. 2010 Jan;18(1):46-54
references.pmid: 19942436
references.citation: Emerg Infect Dis. 2011 Nov;17(11):2122-9
references.pmid: 22099116
references.citation: MMWR Morb Mortal Wkly Rep. 2010 Dec 24;59(50):1637-41
references.pmid: 21178947
references.citation: Methods Mol Biol. 2009;537:113-37
references.pmid: 19378142
references.citation: Bioinformatics. 2010 Dec 15;26(24):3125-6
references.pmid: 20956244
references.citation: Curr Opin Microbiol. 2010 Oct;13(5):646-51
references.pmid: 20851041
references.citation: PLoS One. 2010 Jun 25;5(6):e11147
references.pmid: 20593022
references.citation: N Engl J Med. 2011 Feb 24;364(8):730-9
references.pmid: 21345102
references.citation: N Engl J Med. 2011 Jan 6;364(1):33-42
references.pmid: 21142692
references.citation: BMC Genomics. 2007 Oct 05;8:359
references.pmid: 17919333
references.citation: J Infect Dev Ctries. 2009 Mar 01;3(2):148-51
references.pmid: 19755746
references.citation: Nature. 2000 Aug 3;406(6795):477-83
references.pmid: 10952301
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15442-7
references.pmid: 19720995
references.citation: Nucleic Acids Res. 2003 Apr 15;31(8):2187-95
references.pmid: 12682369
references.citation: Emerg Infect Dis. 2011 Jun;17(6):1124-5
references.pmid: 21749787
references.citation: J Clin Microbiol. 1993 Jan;31(1):22-5
references.pmid: 7678018
references.citation: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references.pmid: 7984417
references.citation: Syst Biol. 2006 Aug;55(4):539-52
references.pmid: 16785212
references.citation: Science. 2011 Jan 28;331(6016):430-4
references.pmid: 21273480
references.citation: BMC Genomics. 2010 Feb 18;11:120
references.pmid: 20167121
references.citation: Jpn J Med Sci Biol. 1977 Oct;30(5):279-82
references.pmid: 599729