title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process.
abstract: BACKGROUND
  This study aimed at verifying the action of multi-walled carbon nanotubes (MWCNT) under the naturally transformable Neisseria meningitidis against two different DNA obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of DNA, causing the escape of the principal vaccination measured worldwide by the capsular switching process.
  
  
  METHODS
  The bacterium receptor strain C2135 was cultivated and had its mutant DNA donor M2 and M6, which received a receptor strain and MWCNT at three different concentrations. The inhibition effect of DNAse on the DNA in contact with nanoparticles was evaluated.
  
  
  RESULTS
  The results indicated an in increase in the transformation capacity of N. meninigtidis in different concentrations of MWCNT when compared with negative control without nanotubes. A final analysis of the interaction between DNA and MWCNT was carried out using Raman Spectroscopy.
  
  
  CONCLUSIONS
  These increases in the transformation capacity mediated by MWCNT, in meningococci, indicate the interaction of these particles with the virulence acquisition of these bacteria, as well as with the increase in the vaccination escape process.
authors.affiliation: LABIOTEC - Biotechnology Laboratory, Department of Biochemistry, Institute of Biology CP6109, University of Campinas - UNICAMP 13083-970, Campinas, SP, Brazil.
authors.forename: Ives B
authors.initials: IB
authors.lastname: Mattos
authors.forename: Danilo A
authors.initials: DA
authors.lastname: Alves
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Hollanda
authors.forename: Helder J
authors.initials: HJ
authors.lastname: Ceragiogli
authors.forename: Vitor
authors.initials: V
authors.lastname: Baranauskas
authors.forename: Marcelo
authors.initials: M
authors.lastname: Lancellotti
mesh_terms: D016667:Bacterial Capsules / Q000235:genetics*; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D037742:Nanotubes, Carbon / Q000009:adverse effects / Q000737:chemistry*; D009345:Neisseria meningitidis / Q000235:genetics*; D013059:Spectrum Analysis, Raman; D014169:Transformation, Bacterial
chemical_list: D037742:Nanotubes, Carbon
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