title: Non-invasive translational Cynomolgus model for studying folliculogenesis and ovulation using color Doppler ultrasonography.
abstract: BACKGROUND
  In women, different events of folliculogenesis can be measured and evaluated using ultrasound (US) technology. The availability of a non-invasive translational non-human primate model to study these processes would represent a major contribution to further advance R&D efforts toward novel therapies in assisted reproduction.
  
  
  METHODS
  In our study, follicular growth and ovulation was measured in six cyclic Cynomolgus monkeys using abdominal Doppler US.
  
  
  RESULTS
  The mean follicular diameter on cycle day -6 (cycle day 0=day of ovulation) was 3.7mm that increased to 6.8mm on cycle day -1. After ovulation, the mean diameter decreased to 4.6mm, confirming ovulation. The mean percentage of follicular size reduction after ovulation was 31%.
  
  
  CONCLUSIONS
  Ultrasonography in combination with color-flow Doppler imaging was shown to be a useful, non-invasive translational method to measure ovarian follicular growth and occurrence and timing of follicular rupture in Cynomolgus monkeys.
authors.affiliation: Women's Health Department, Merck Research Laboratories, Oss, The Netherlands. havandiepen@gmail.com
authors.forename: Harry A
authors.initials: HA
authors.lastname: van Diepen
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Pansier
authors.forename: Paul
authors.initials: P
authors.lastname: Oude Wesselink
authors.forename: Aric
authors.initials: A
authors.lastname: van Drie
authors.forename: Marcel
authors.initials: M
authors.lastname: van Duin
authors.forename: Sabine
authors.initials: S
authors.lastname: Mulders
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D008252:Macaca fascicularis / Q000097:blood / Q000502:physiology*; D023421:Models, Animal; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D018615:Ultrasonography, Doppler, Color
chemical_list: D011374:Progesterone; D004958:Estradiol