title: RNA interference inhibits replication of tick-borne encephalitis virus in vitro.
abstract: Each year, up to 10,000 cases of infections with the flavivirus tick-borne encephalitis (TBE) virus that affect the central nervous system are reported in Europe and Asia. Due to the potentially severe adverse effects of post-exposure prophylaxis with TBE virus hyperimmunoglobulin, TBE can currently only be treated symptomatically. An RNA interference (RNAi) approach to inhibit TBE virus replication was therefore developed. In this study we demonstrate for the first time that small interfering RNAs (siRNAs) targeted at the TBE virus genome reduce the quantity of infectious TBE virus particles, TBE virus genome, and TBE virus protein in vitro by up to 85%. The 50% inhibitory dose (DI(50)) of the shRNA plasmid was only 0.05μg/ml. As RNAi-based therapeutics for other diseases are already being evaluated in phases II and III clinical trials, it is possible that RNAi could become valuable tool for controlling TBE virus infection.
authors.affiliation: Centre for Biological Security 1 (ZBS 1), Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany. AchaziK@rki.de
authors.forename: Katharina
authors.initials: K
authors.lastname: Achazi
authors.forename: Pranav
authors.initials: P
authors.lastname: Patel
authors.forename: Ravish
authors.initials: R
authors.lastname: Paliwal
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Radonić
authors.forename: Matthias
authors.initials: M
authors.lastname: Niedrig
authors.forename: Oliver
authors.initials: O
authors.lastname: Donoso-Mantke
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000032:analysis; D014779:Virus Replication / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D014759:Viral Envelope Proteins