title: Studying the interactions of a platinum(II) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS.
abstract: The interaction of a novel Pt complex, [Pt(dmba)(N9-9AA)(PPh(3))](+)1 (dmba = N,N-dimethylbenzylamine-κN,κC; 9AA = 9-aminoacridine), which exhibits anti-tumor activity, with certain key proteins has been monitored by ESI-MS. Also, the interaction of 1 with a designed double-stranded oligonucleotide containing the GG motif has been followed by mass spectrometry as well as by fluorimetry. The results obtained show the low interaction of 1 with the considered proteins and the absence of covalent interaction with the oligonucleotides, but the fluorimetric data confirm the π-π interaction of 1 with the double-stranded DNA, which is probably the reason of the previously reported activity of 1 in several tumor cell lines.
authors.affiliation: Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193-Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Katia G
authors.initials: KG
authors.lastname: Samper
authors.forename: Consuelo
authors.initials: C
authors.lastname: Vicente
authors.forename: Venancio
authors.initials: V
authors.lastname: Rodríguez
authors.forename: Sílvia
authors.initials: S
authors.lastname: Atrian
authors.forename: Natalia
authors.initials: N
authors.lastname: Cutillas
authors.forename: Mercè
authors.initials: M
authors.lastname: Capdevila
authors.forename: José
authors.initials: J
authors.lastname: Ruiz
authors.forename: Òscar
authors.initials: Ò
authors.lastname: Palacios
mesh_terms: D000585:Aminacrine / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009841:Oligonucleotides; D011506:Proteins; D010984:Platinum; D000585:Aminacrine