title: Prolonged release from PLGA/HAp scaffolds containing drug-loaded PLGA/gelatin composite microspheres.
abstract: Porous scaffolds that can prolong the release of bioactive factors are urgently required in bone tissue engineering. In this study, PLGA/gelatin composite microspheres (PGMs) were carefully designed and prepared by entrapping poly(L: -lactide-co-glycolide) (PLGA) microspheres (PMs) in gelatin matrix. By mixing PGMs with PLGA solution directly, drug-loaded PLGA/carbonated hydroxyapatite (HAp)/PGMs composite scaffolds were successfully fabricated. In vitro release of fluorescein isothiocyanate-dextran (FD70S) as a model drug from the scaffolds as well as PMs and PGMs was studied by immersing samples in phosphate buffered saline (pH = 7.4) at 37°C for 32 days. Compared with PMs, PGMs and PLGA/HAp/PGMs scaffolds exhibited slow and steady release behavior with constant release rate and insignificantly original burst release. The swelling of PGMs, diffusion of drugs, and degradation of polymer dominated the release behaviors synergistically. The PLGA/HAp/PGMs scaffold offers a novel option for sequential or simultaneous release of several drugs in terms of bone regeneration.
authors.affiliation: School of Materials Science and Engineering, and Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, China.
authors.forename: Gongwen
authors.initials: G
authors.lastname: Tang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yuan
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D001861:Bone Regeneration; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry; D015195:Drug Design; D017886:Durapatite / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008863:Microspheres; D010710:Phosphates / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D010710:Phosphates; D011108:Polymers; D012492:Salts; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin; D017886:Durapatite; D016650:Fluorescein-5-isothiocyanate
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