title: Human keratinocytes express AIM2 and respond to dsDNA with IL-1β secretion.
abstract: Keratinocytes have been recognized to actively participate in the skin immune response. It has been shown that keratinocytes express all components that are necessary to form the NLRP3 inflammasome complex including the adapter protein ASC and caspase-1. In this study, we investigated the presence and activity of the recently identified absent in melanoma 2 (AIM2) inflammasome in human keratinocytes. We were able to show that an AIM2 inflammasome is active in human keratinocytes. IL-1 production by keratinocytes plays a pivotal role in inflammatory processes in the skin. Activation of the AIM2 inflammasome in keratinocytes represents another potential trigger factor for the development and maintenance of inflammatory skin diseases.
authors.forename: Verena
authors.initials: V
authors.lastname: Kopfnagel
authors.forename: Miriam
authors.initials: M
authors.lastname: Wittmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Werfel
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism; D061945:Caspase Inhibitors; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D003876:Dermatitis, Atopic / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology; D006801:Humans; D058847:Inflammasomes / Q000187:drug effects / Q000378:metabolism; D020382:Interleukin-18 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010873:Pinocytosis / Q000187:drug effects; D011565:Psoriasis / Q000378:metabolism
chemical_list: C410167:AIM2 protein, human; D000590:Amino Acid Chloromethyl Ketones; D061945:Caspase Inhibitors; D004268:DNA-Binding Proteins; D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta; D009687:Nuclear Proteins; C460579:benzyloxycarbonyltyrosyl-valyl-alanyl-aspartic acid fluoromethyl ketone; D000584:Amiloride; D004247:DNA; D020170:Caspase 1