title: Sequence analysis of 12 genome segments of mud crab reovirus (MCRV).
abstract: Mud crab reovirus (MCRV) is the causative agent of a serious disease with high mortality in cultured mud crab (Scylla serrata). This study sequenced and analyzed 12 genome segments of MCRV. The 12 genome segments had a total length of 24.464 kb, showing a total G+C content of 41.29% and predicted 15 ORFs. Sequence analysis showed that the majority of MCRV genes shared low homology with the counterpart genes of other reoviruses, e.g., the amino acid identity of RNA-dependent RNA polymerase (RdRp) was lower than 13.0% compared to the RdRp sequences of other reoviruses. Nucleotide and amino acid sequences of RdRp and capping enzyme suggested MCRV as a single group. Further genome-based phylogenetical analysis of conserved termini and reovirus polymerase motif indicates that this MCRV belongs to a new genus of the Reoviridae family, tentatively named as Crabreovirus.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People's Republic of China.
authors.forename: Xie-xiong
authors.initials: XX
authors.lastname: Deng
authors.forename: Ling
authors.initials: L
authors.lastname: Lü
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Ou
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Su
authors.forename: Guang
authors.initials: G
authors.lastname: Li
authors.forename: Zhi-xun
authors.initials: ZX
authors.lastname: Guo
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Pei-rui
authors.initials: PR
authors.lastname: Zheng
authors.forename: Yong-gui
authors.initials: YG
authors.lastname: Chen
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: He
authors.forename: Shao-ping
authors.initials: SP
authors.lastname: Weng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral