title: Vimentin and laminin are altered on cheek pouch microvessels of streptozotocin-induced diabetic hamsters.
abstract: OBJECTIVE
  Normal endothelial cells respond to shear stress by elongating and aligning in the direction of fluid flow. Hyperglycemia impairs this response and contributes to microvascular complications, which result in deleterious effects to the endothelium. This work aimed to evaluate cheek pouch microvessel morphological characteristics, reactivity, permeability, and expression of cytoskeleton and extracellular matrix components in hamsters after the induction of diabetes with streptozotocin.
  
  
  METHODS
  Syrian golden hamsters (90-130 g) were injected with streptozotocin (50 mg/kg, i.p.) or vehicle either 6 (the diabetes mellitus 6 group) or 15 (the diabetes mellitus 15 group) days before the experiment. Vascular dimensions and density per area of vessels were determined by morphometric and stereological measurements. Changes in blood flow were measured in response to acetylcholine, and plasma extravasation was measured by the number of leakage sites. Actin, talin, α-smooth muscle actin, vimentin, type IV collagen, and laminin were detected by immunohistochemistry and assessed through a semiquantitative scoring system.
  
  
  RESULTS
  There were no major alterations in the lumen, wall diameters, or densities of the examined vessels. Likewise, vascular reactivity and permeability were not altered by diabetes. The arterioles demonstrated increased immunoreactivity to vimentin and laminin in the diabetes mellitus 6 and diabetes mellitus 15 groups.
  
  
  CONCLUSIONS
  Antibodies against laminin and vimentin inhibit branching morphogenesis in vitro. Therefore, laminin and vimentin participating in the structure of the focal adhesion may play a role in angiogenesis.
  
  
  CONCLUSIONS
  Our results indicated the existence of changes related to cell-matrix interactions, which may contribute to the pathological remodeling that was already underway one week after induction of experimental diabetes.
authors.affiliation: Laboratory of Cellular Ultrastructure and Tissue Biology, Biomedical Center, Institute of Biology, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Jemima Fuentes R
authors.initials: JF
authors.lastname: Silva
authors.forename: Fatima Z G A
authors.initials: FZ
authors.lastname: Cyrino
authors.forename: Marisa M D
authors.initials: MM
authors.lastname: Breitenbach
authors.forename: Eliete
authors.initials: E
authors.lastname: Bouskela
authors.forename: Jorge José
authors.initials: JJ
authors.lastname: Carvalho
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000473:pathology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002610:Cheek / Q000098:blood supply; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D006632:Histamine / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism / Q000648:ultrastructure*; D008297:Male; D008647:Mesocricetus; D055806:Microvessels / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*; D014746:Vimentin / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D007797:Laminin; D014665:Vasodilator Agents; D014746:Vimentin; D006632:Histamine; D000109:Acetylcholine
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