title: Cell adhesion to plasma electrolytic oxidation (PEO) titania coatings, assessed using a centrifuging technique.
abstract: The adhesion of bovine chondrocytes and human osteoblasts to three titania-based coatings, formed by plasma electrolytic oxidation (PEO), was compared to that on uncoated Ti-6Al-4V substrates, and some comparisons were also made with plasma sprayed hydroxyapatite (HA) coatings. This was done using a centrifuge, with accelerations of up to 160,000 g, so as to induce buoyancy forces that created normal or shear stresses at the interface. It is shown that, on all surfaces, it was easier to remove cells under normal loading than under shear loading. Cell adhesion to the PEO coatings was stronger than that on Ti-6Al-4V and similar to that on HA. Cell proliferation rates were relatively high on one of the PEO coatings, which was virtually free of aluminium, but low on the other two, which contained significant levels of aluminium. It is concluded that the Al-free PEO coating offers promise for application to prosthetic implants.
authors.affiliation: Department of Materials Science & Metallurgy, Cambridge University, Pembroke Street, Cambridge CB2 3QZ, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Robinson
authors.forename: A E
authors.initials: AE
authors.lastname: Markaki
authors.forename: C A
authors.initials: CA
authors.lastname: Collier
authors.forename: T W
authors.initials: TW
authors.lastname: Clyne
mesh_terms: D000497:Alloys; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002498:Centrifugation / Q000379:methods*; D019902:Chondrocytes / Q000166:cytology; D004572:Electrolysis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology; D010084:Oxidation-Reduction; D058626:Plasma Gases / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000497:Alloys; D058626:Plasma Gases; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; D014025:Titanium