title: Anti-adhesive functions of CD43 expressed on colon carcinoma cells through the modulation of integrins.
abstract: CD43 has conflicting roles in both pro- and anti-adhesive function in cell-to-cell adhesion in hematopoietic cells. We examined the role of CD43 glycoprotein in a colorectal carcinoma cell line. We expressed human CD43 antigen on HT-29 cells, a colon adenocarcinoma cell line, and compared the adhesion to the extracellular matrix with that of mock-transduced cells in vitro. CD43 expression inhibited the adhesion to extracellular matrix, such as collagen type IV and laminin. As the expression of β1 integrin was downregulated in CD43-expressing HT-29 cells, the anti-adhesive effect of CD43 might be implicated in its expression. Our findings suggest that the anti-adhesive function of CD43 in colon carcinoma cells plays a role in the tumorigenesis and metastasis of colorectal carcinoma cells.
authors.affiliation: Hematologic Malignancies Branch, National Cancer Center, 809 Madu-dong, Ilsan-gu, Goyang, Gyeonggi 411-769, Republic of Korea.
authors.forename: Weon Seo
authors.initials: WS
authors.lastname: Park
authors.forename: Hae Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Geon Kook
authors.initials: GK
authors.lastname: Lee
authors.forename: Hyeon S
authors.initials: HS
authors.lastname: Son
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Bae
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic; D024141:Collagen Type IV / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D051917:Leukosialin / Q000378:metabolism*; D009362:Neoplasm Metastasis
chemical_list: D024141:Collagen Type IV; D016023:Integrins; D007797:Laminin; D051917:Leukosialin