title: Decidualized pseudopregnant rat uterus shows marked reduction in Ang II and Ang-(1-7) levels.
abstract: Previous studies showed that angiotensin (Ang) II and Ang-(1-7) concentrations were reduced in the implantation site at day 7 of pregnancy in Sprague-Dawley rats as compared to the site immediately adjacent to it, which does not have the embryo attached, clearly showing the importance of the blastocyst in the regulation of renin-angiotensin system (RAS).
  
  
  OBJECTIVE
  The objective of this study was to evaluate the regulation of the RAS in the decidualized uterus in the pseudopregnant rat, a model without the presence of a conceptus.
  
  
  METHODS
  Ovariectomized, adult female rats were sensitized for the decidual cell reaction with steroid treatments; decidualization was induced by oil-injection of the right horn; the left horn served as a control. The uterine content of Ang I, Ang II, and Ang-(1-7) was examined in the decidualized and non-decidualized uteri.
  
  
  RESULTS
  Both Ang-(1-7) and Ang II and ACE and ACE2 mRNA were significantly reduced in the decidualized horn as compared to the non-decidualized horn. Immunocytochemical characterization of Ang II, Ang-(1-7), ACE and ACE2 demonstrated that Ang-(1-7), Ang II, and ACE2 polarize to the anti-mesometrial pole with decidualization.
  
  
  CONCLUSIONS
  The decidualization process elicits marked reduction in uterine Ang II and Ang-(1-7) content as compared to the non-decidualized horn. The differential immunocytochemical expression of Ang II and Ang-(1-7) with ACE2, but not ACE in the anti-mesometrial pole of the decidualized horn may favor the formation and action of Ang-(1-7) in the anti-mesometrial pole, an area which plays a role in triggering the decidualization process.
authors.affiliation: Hypertension and Vascular Research Center, Wake Forest School of Medicine, Winston-Salem, NC 27157, United States. bbrosnih@wakehealth.edu
authors.forename: K B
authors.initials: KB
authors.lastname: Brosnihan
authors.forename: M S
authors.initials: MS
authors.lastname: Bharadwaj
authors.forename: L M
authors.initials: LM
authors.lastname: Yamaleyeva
authors.forename: L A A
authors.initials: LA
authors.lastname: Neves
mesh_terms: D000803:Angiotensin I / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000804:Angiotensin II / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D016764:Cell Polarity; D003656:Decidua / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D015536:Down-Regulation; D010064:Embryo Implantation; D005260:Female; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D010446:Peptide Fragments / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011555:Pseudopregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System; D014599:Uterus / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D012333:RNA, Messenger; D000804:Angiotensin II; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; C000705329:Ace2 protein, rat; D000085962:Angiotensin-Converting Enzyme 2; C118790:angiotensin I (1-7)
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