title: Decreased nucleotide excision repair in steatotic livers associates with myeloperoxidase-immunoreactivity.
abstract: Chronic inflammation is characterized by the influx of neutrophils and is associated with an increased production of reactive oxygen species that can damage DNA. Oxidative DNA damage is generally thought to be involved in the increased risk of cancer in inflamed tissues. We previously demonstrated that activated neutrophil mediated oxidative stress results in a reduction in nucleotide excision repair (NER) capacity, which could further enhance mutagenesis. Inflammation and oxidative stress are critical factors in the progression of nonalcoholic fatty liver disease that is linked with enhanced liver cancer risk. In this report, we therefore evaluated the role of neutrophils and the associated oxidative stress in damage recognition and DNA repair in steatotic livers of 35 severely obese subjects with either nonalcoholic steatohepatitis (NASH) (n=17) or steatosis alone (n=18). The neutrophilic influx in liver was assessed by myeloperoxidase (MPO) staining and the amount of oxidative DNA damage by measuring M(1)dG adducts. No differences in M(1)dG adduct levels were observed between patients with or without NASH and also not between individuals with high or low MPO immunoreactivity. However, we found that high expression of MPO in the liver, irrespective of disease status, reduced the damage recognition capacity as determined by staining for histone 2AX phosphorylation (γH2AX). This reduction in γH2AX formation in individuals with high MPO immunoreactivity was paralleled by a significant decrease in NER capacity as assessed by a functional repair assay, and was not related to cell proliferation. Thus, the observed reduction in NER capacity upon hepatic inflammation is associated with and may be a consequence of reduced damage recognition. These findings suggest a novel mechanism of liver cancer development in patients with nonalcoholic fatty liver disease.
authors.affiliation: Department of Toxicology, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Marten A
authors.initials: MA
authors.lastname: Schults
authors.forename: Peter W
authors.initials: PW
authors.lastname: Nagle
authors.forename: Sander S
authors.initials: SS
authors.lastname: Rensen
authors.forename: Roger W
authors.initials: RW
authors.lastname: Godschalk
authors.forename: Armelle
authors.initials: A
authors.lastname: Munnia
authors.forename: Marco
authors.initials: M
authors.lastname: Peluso
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Claessen
authors.forename: Jan W
authors.initials: JW
authors.lastname: Greve
authors.forename: Ann
authors.initials: A
authors.lastname: Driessen
authors.forename: Froukje J
authors.initials: FJ
authors.lastname: Verdam
authors.forename: Wim A
authors.initials: WA
authors.lastname: Buurman
authors.forename: Frederik J
authors.initials: FJ
authors.lastname: van Schooten
authors.forename: Roland K
authors.initials: RK
authors.lastname: Chiu
mesh_terms: D000328:Adult; D004249:DNA Damage; D004260:DNA Repair; D005234:Fatty Liver / Q000235:genetics; D005260:Female; D006657:Histones / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D009765:Obesity / Q000150:complications; D018384:Oxidative Stress / Q000235:genetics; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C468783:H2AX protein, human; D006657:Histones; D017382:Reactive Oxygen Species; D009195:Peroxidase