title: Oceanicaulis stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata.
abstract: A bacterial strain designated GISW-4(T) was isolated from the reef-building coral Stylophora pistillata, collected from seawater off the coast of southern Taiwan, and was characterized in this taxonomic study using a polyphasic approach. Strain GISW-4(T) was Gram-stain-negative, aerobic, beige, rod-shaped, and dimorphic, either non-motile with stalks (or prosthecae), or non-stalked and motile by means of a single polar flagellum. 16S rRNA gene sequence studies showed that the novel strain clustered with Oceanicaulis alexandrii C116-18(T) (98.9 % 16S rRNA gene sequence similarity). Strain GISW-4(T) exhibited optimal growth at 35-40 °C, 1-2 % (w/v) NaCl and at pH 7-9. The predominant cellular fatty acids (>10 %) were C(18 : 0), C(18 : 1)ω7c and C(18 : 1)ω7c 11-methyl. The predominant polar lipids were phosphatidylglycerol, sulfoquinovosyl diacylglycerol and two unknown phospholipids (PL1-2). The major respiratory quinones were ubiquinone Q-10 and Q-9, and the DNA G+C content was 61.6 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain GISW-4(T) from the type strain of O. alexandrii. It is evident from the genotypic, phenotypic and chemotaxonomic data that the isolate should be classified as a novel species of the genus Oceanicaulis. The name proposed for this taxon is Oceanicaulis stylophorae sp. nov., with the type strain GISW-4(T) (= LMG 25723(T) = BCRC 80207(T)).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Research Center for Biodiversity, Academia Sinica, Nangang 115, Taipei, Taiwan, ROC.
authors.forename: Chaolun Allen
authors.initials: CA
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, Tajen University, Yanpu, Pingtung 907, Taiwan, ROC.
authors.forename: Jih-Terng
authors.initials: JT
authors.lastname: Wang
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone