title: Evaluation of hemostatic balance in blood from patients with polycythemia vera by means of thromboelastography: the effect of isovolemic erythrocytapheresis.
abstract: Polycythemia vera (PV) is associated with an increased frequency of thrombotic complications. This study was undertaken to evaluate the hemostatic balance in the blood of PV patients by means of thromboelastography (TEG). The effect of isovolemic erythrocytapheresis (ECP) on the hemostasis of PV patients was also studied. We assessed the coagulation status of 76 PV patients undergoing ECP and 50 of healthy controls. TEG measurements were performed immediately before and after the ECP procedure. Coagulation was triggered by recalcification in freshly collected citrated blood. We recorded clotting time (R), alpha angle, and maximum amplitude (MA) of the clot. The results presented here show that, compared with healthy controls, PV patients demonstrated an increase in alpha angle (p<0.005) and in MA (p=0.14). In the subgroup of PV patients with high (>440 × 10(9)l(-1)) platelet (PLT) count, differences in MA (p<0.01) and alpha angle (p<0.001) were more significant. Following ECP procedure, a significant (p ≤ 0.01) reduction of R time, a rise of alpha angle, and MA were observed, indicating augmentation of a hypercoagulable state. In PV patients, the rise in alpha angle positively correlated (r=0.549) with platelet count but not with the number of erythrocytes and leukocytes. Following ECP, this correlation was reduced (r=0.382). Dilution (with saline) of blood from PV patients and of healthy controls, to a degree similar to that used during the ECP procedure, resulted in reduction of R and rise of the alpha angle. In conclusion, TEG measurements show that the majority of PV patients demonstrate abnormal hemostasis in which a major role is played by platelets rather than plasma factors. The hypercoagulable state in PV patients is significantly augmented following the ECP and may be related to the hemodilution intrinsically included in this procedure. TEG may help to assess the thrombotic risk in individual PV patients.
authors.affiliation: Department of Physical Chemistry, Medical University of Bialystok ,  Bialystok ,  Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rusak
authors.forename: Michal
authors.initials: M
authors.lastname: Ciborowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Uchimiak-Owieczko
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Piszcz
authors.forename: Piotr
authors.initials: P
authors.lastname: Radziwon
authors.forename: Marian
authors.initials: M
authors.lastname: Tomasiak
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001772:Blood Cell Count; D001780:Blood Coagulation Tests; D001781:Blood Component Removal / Q000379:methods*; D016022:Case-Control Studies; D017707:Erythrocyte Transfusion / Q000379:methods*; D005260:Female; D006487:Hemostasis; D006801:Humans; D008297:Male; D008875:Middle Aged; D011087:Polycythemia Vera / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000473:pathology; D012307:Risk Factors; D013916:Thrombelastography; D013927:Thrombosis / Q000097:blood / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D014914:Whole Blood Coagulation Time