title: Tissue repair driven by two different mechanisms of growth factor plasmids VEGF and NGF in mice auricular cartilage: regeneration mediated by administering growth factor plasmids.
abstract: The focus of this study was to compare the role of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in the regeneration of experimental skin and cartilage trauma. The role of VEGF in this process is known since decade; the NGF participation on this process has been first discussed within the spinal cord injury repair. We hypothesized that both VEGF and NGF induce angiogenesis and take part on the repair process. The angiogenesis response and the cartilage regeneration after phVEGF(165) plasmid and rat pcNGF plasmid administration were investigated using BALB/c mice. PhVEGF(165) and pcNFG were injected into the right mice ear and plain vector injection into the left ear the day before trauma. The next day, all mice were ear-punched, resulting in 2-mm diameter puncture through the center of both pinnae. In BALB/c mouse strain, a significantly faster cartilage repair was observed after phVEGF(165) and pcNGF injection into punched ear area in comparison to the control group. It has been shown that the healing process is after VEGF and NGF injection driven differentially. In case of VEGF is the cartilage wound repaired by induction of new chondrocytes differentiation. In the case of NGF, the regeneration is supported by immature leukocytes attracted into the punched area. The leukocytes induct angiogenesis so far indirectly by inflammation. The NGF-induced inflammation environment may be a part of mosaic creating the complete picture of regeneration.
authors.affiliation: Department of Tumor Biology, Third Faculty of Medicine, Charles University Prague, Ruska 87, 100 34 Prague 10, Czech Republic.
authors.forename: Katarina
authors.initials: K
authors.lastname: Kolostova
authors.forename: Oliver
authors.initials: O
authors.lastname: Taltynov
authors.forename: Daniela
authors.initials: D
authors.lastname: Pinterova
authors.forename: Martin
authors.initials: M
authors.lastname: Cegan
authors.forename: Lenka
authors.initials: L
authors.lastname: Ceganova
authors.forename: Marie
authors.initials: M
authors.lastname: Jirkovska
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bobek
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage; D000818:Animals; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology; D004425:Ear Cartilage / Q000293:injuries / Q000502:physiology; D005822:Genetic Vectors; D007249:Inflammation / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000235:genetics; D010957:Plasmids; D051381:Rats; D012867:Skin / Q000293:injuries; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents; D009414:Nerve Growth Factors; D042461:Vascular Endothelial Growth Factor A
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