title: [Analysis of genetic diversity and amino acid sequence of HIV-1tat in CNS and peripheral tissue of a patient with ADC and a patient with non-ADC].
abstract: OBJECTIVE
  To study the diversity of HIV-1 tat gene in CNS and peripheral tissue of a patient with ADC and a patient with non-ADC, so as to research HIV evolution, the mechanism of CNS invasion and the pathogenesis of ADC.
  
  
  METHODS
  The tat gene was amplified with nested PCR from genomic DNA which was extracted from spleen and basal ganglia of one non-ADC patient with a wide range of cerebral artery atherosclerosis and one ADC patient. PCR products were cloned into the PGEM-T vector, after transformation and selection by ampicillin and blue/white spotting. Five of positive clones were sequenced. HIV-1 tat sequences were processed with BioEdit and MEGA4. With the softwares, neighbor-joining tree, p-distances, values of ds/dn, and analysis of amino acid motifs were all done, so as to research the diversity of HIV-1 tat gene in CNS and peripheral tissue.
  
  
  RESULTS
  Gene mutation of HIV-1 tat exist in the two patients, the mutation process of tat isolated from ADC patient suffered more compartmentalization than tat isolated from non-ADC patient, the differences of tat genes between CNS and peripheral tissue in ADC patient were greater than the non-ADC patient. Ds/dn showed that the virus gene mutation played a major role, the body intend to remove harmful non-synonymous mutations.
  
  
  CONCLUSIONS
  The compartmentation of tat gene in CNS and peripheral tissue of the two patients was different, the reason may be related to the pathway of HIV into the CNS, the relationship between HIV gene mutation in CNS and ADC still need more investigation.
authors.affiliation: Department of Laboratory, First Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jian, China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Pu
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Li
authors.forename: Yu-fen
authors.initials: YF
authors.lastname: Yan
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Wen
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Song
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
mesh_terms: D015526:AIDS Dementia Complex / Q000821:virology*; D000328:Adult; D000595:Amino Acid Sequence; D002490:Central Nervous System / Q000821:virology*; D005260:Female; D014644:Genetic Variation; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D017933:Peripheral Nervous System / Q000821:virology*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054322:tat Gene Products, Human Immunodeficiency Virus