title: Structures and anti-HSV-2 activities of neutral polysaccharides from an edible plant, Basella rubra L.
abstract: Four neutral polysaccharides (BRN-1, BRN-2, BRN-3 and BRN-4) were isolated from the hot water extract of the aerial part of Basella rubra L. They were found to consist of a large amount of D-galactose (81.0-92.4%) and small amounts of L-arabinose (5.4-7.8%), D-glucose (2.2-11.0%) and mannose (~2.9%). Linkage analysis revealed that all these neutral polysaccharides might be arabinogalactan type I polysaccharides in different molecular weight and chain length. Among them, only BRN-3 showed antiviral activity against herpes simplex virus type 2 (HSV-2) with 50% inhibitory concentration of 55 μg/mL without showing the cytotoxicity up to 2300 μg/mL. Furthermore, the main antiviral target of BRN-3 was shown to be the inhibition of virus adsorption to host cells. This is the first report on the neutral polysaccharide with anti-HSV-2 activity obtained from B. rubra L.
authors.affiliation: Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Sugitani, Toyama, Japan.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Dong
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mizukoshi
authors.forename: Jung-Bum
authors.initials: JB
authors.lastname: Lee
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002241:Carbohydrates / Q000737:chemistry; D002522:Chlorocebus aethiops; D003124:Colorimetry / Q000379:methods; D004285:Dogs; D019669:Embryophyta / Q000378:metabolism*; D005685:Galactans / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D009980:Influenza A virus / Q000378:metabolism; D008970:Molecular Weight; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*; D013234:Stem Cells; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D002241:Carbohydrates; D005685:Galactans; D010936:Plant Extracts; D011134:Polysaccharides; D005947:Glucose; C005653:arabinogalactan; D005690:Galactose