title: Impact of dietary fiber coatings on behavior of protein-stabilized lipid droplets under simulated gastrointestinal conditions.
abstract: Multilayer emulsions containing lipid droplets coated by lactoferrin (LF) - anionic polysaccharide layers have improved resistance to environmental stresses (such as pH, salt, and temperature), but their behavior within the gastrointestinal tract (GIT) is currently unknown. The objective of this research was therefore to monitor changes in the physicochemical properties and digestibility of these systems under simulated GIT conditions. Primary emulsions (5% corn oil, 0.5% LF) were prepared using a high-pressure homogenizer. Secondary emulsions (5% corn oil, 0.5% LF, 0.5% polysaccharide) were prepared by incorporating alginate, low methoxyl pectin (LMP) or high methoxyl pectin (HMP) into primary emulsions. Emulsions were then subjected to simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) conditions in sequence. LF, LF-LMP and LF-HMP emulsions were stable to droplet aggregation in the stomach but aggregated in the small intestine, whereas LF-alginate emulsions aggregated in both the stomach and small intestine. The presence of a dietary fiber coating around the initial lipid droplets had little influence on the total extent of lipid digestion in SIF, but LF-alginate emulsions had a slower initial digestion rate than the other emulsions. These results suggest that the dietary fiber coatings may become detached in the small intestine, or that they were permeable to digestive enzymes. Pepsin was found to have little influence on the physical stability or digestibility of the emulsions. The knowledge obtained from this study is important for the design of delivery systems for encapsulation and release of lipophilic bioactive ingredients.
authors.affiliation: Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA.
authors.forename: Tanushree
authors.initials: T
authors.lastname: Tokle
authors.forename: Uri
authors.initials: U
authors.lastname: Lesmes
authors.forename: Eric Andrew
authors.initials: EA
authors.lastname: Decker
authors.forename: David Julian
authors.initials: DJ
authors.lastname: McClements
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D003314:Corn Oil / Q000737:chemistry* / Q000378:metabolism; D004043:Dietary Fiber / Q000032:analysis* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000378:metabolism*; D007781:Lactoferrin / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D010316:Particle Size; D013552:Swine
chemical_list: D004043:Dietary Fiber; D004655:Emulsions; D003314:Corn Oil; D007781:Lactoferrin