title: Leishmanicidal and antitumoral activities of endophytic fungi associated with the Antarctic angiosperms Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl.
abstract: A total of 564 isolates of endophytic fungi were recovered from the plants Deschampsia antarctica and Colobanthus quitensis collected from Antarctica. The isolates were screened against parasites Leishmania amazonensis and Trypanosoma cruzi and against the human tumour cell lines. Of the 313 fungal isolates obtained from D. antarctica and 251 from C. quitensis, 25 displayed biological activity. Nineteen extracts displayed leishmanicidal activity, and six inhibited the growth of at least one tumour cell line. These fungi belong to 19 taxa of the genera Alternaria, Antarctomyces, Cadophora, Davidiella, Helgardia, Herpotrichia, Microdochium, Oculimacula, Phaeosphaeria and one unidentified fungus. Extracts of 12 fungal isolates inhibited the proliferation of L. amazonesis at a low IC(50) of between 0.2 and 12.5 μg ml(-1). The fungus Phaeosphaeria herpotrichoides displayed only leishmanicidal activity with an IC(50) of 0.2 μg ml(-1), which is equivalent to the inhibitory value of amphotericin B. The extract of Microdochium phragmitis displayed specific cytotoxic activity against the UACC-62 cell line with an IC(50) value of 12.5 μg ml(-1). Our results indicate that the unique angiosperms living in Antarctica shelter an interesting bioactive fungal community that is able to produce antiprotozoal and antitumoral molecules. These molecules may be used to develop new leishmanicidal and anticancer drugs.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Iara F
authors.initials: IF
authors.lastname: Santiago
authors.forename: Tânia M A
authors.initials: TM
authors.lastname: Alves
authors.forename: Ana
authors.initials: A
authors.lastname: Rabello
authors.forename: Policarpo A
authors.initials: PA
authors.lastname: Sales Junior
authors.forename: Alvaro J
authors.initials: AJ
authors.lastname: Romanha
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Zani
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
authors.forename: Luiz H
authors.initials: LH
authors.lastname: Rosa
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D029748:Caryophyllaceae / Q000382:microbiology*; D045744:Cell Line, Tumor; D060026:Endophytes / Q000737:chemistry / Q000502:physiology*; D005658:Fungi / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D020128:Inhibitory Concentration 50; D007891:Leishmania; D009369:Neoplasms; D006109:Poaceae / Q000382:microbiology*
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