title: Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.
abstract: A dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.4, b = 119.4, c = 70.2 Å, β = 112.0°, and diffracted to 2.0 Å resolution on the BL26B1 beamline at SPring-8. The overall R(merge) was 4.5% and the completeness was 99.8%.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Kita-gun, Kagawa 761-0795, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Shibahara
authors.forename: Takenori
authors.initials: T
authors.lastname: Satomura
authors.forename: Ryushi
authors.initials: R
authors.lastname: Kawakami
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
mesh_terms: D041522:Aeropyrum / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D045022:Lactate Dehydrogenases / Q000737:chemistry*
chemical_list: D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase
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