title: DNA concentration modulation on supported lipid bilayers switched by surface acoustic waves.
abstract: Spatially addressable arrays of molecules embedded in or anchored to supported lipid bilayers are important for on-chip screening and binding assays; however, methods to sort or accumulate components in a fluid membrane on demand are still limited. Here we apply in-plane surface acoustic shear waves (SAWs) to laterally accumulate double-stranded DNA segments electrostatically bound to a cationic supported lipid bilayer. The fluorescently labeled DNA segments are found to segregate into stripe patterns with a spatial frequency corresponding to the periodicity of the standing SAW wave (~10 μm). The DNA molecules are accumulated 10-fold in the regions of SAW antinodes. The superposition of two orthogonal sets of SAW sources creates checkerboard like arrays of DNA demonstrating the potential to generate arrayed fields dynamically. The pattern relaxation time of 0.58 s, which is independent of the segment length, indicates a sorting and relaxation mechanism dominated by lipid diffusion rather than DNA self-diffusion.
authors.affiliation: Center for NanoScience, Ludwig-Maximilians-Universität, Fakultät für Physik, Geschwister Scholl Platz 1, D-80539 München, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hennig
authors.forename: Manuel
authors.initials: M
authors.lastname: Wolff
authors.forename: Jürgen
authors.initials: J
authors.lastname: Neumann
authors.forename: Achim
authors.initials: A
authors.lastname: Wixforth
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Schneider
authors.forename: Joachim O
authors.initials: JO
authors.lastname: Rädler
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004058:Diffusion; D005453:Fluorescence; D056656:Lab-On-A-Chip Devices; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D013016:Sound; D055672:Static Electricity; D014966:Xanthenes / Q000032:analysis
chemical_list: D008051:Lipid Bilayers; D014966:Xanthenes; C034657:Texas red; D004247:DNA