title: [Different expression of TGF-beta1 and collagen during the healing process of wound infected by Pseudomonas aeruginosa].
abstract: OBJECTIVE
  To explore the different expression of TGF-beta1 and collagen during the healing process of wound infected by Pseudomonas aeruginosa (PAO1).
  
  
  METHODS
  24 female Wistar rats were randomly divided into pure wound group (group A) and wound + PAO1 group (group B). The re-epithelial rate, shrinkage rate and neutrophils number on the wounds were observed on the 1st, 3rd, 7th and 10th day after operation. The expression of TGF-beta1 and collage I, Ill was also detected.
  
  
  RESULTS
  On the 7th day, the re-epithelial rate in group A was higher than that in group B, while the shrinkage rate in group A was lower than that in group B. The neutrophils number increased to peak on the 1st day in group B, but on the 3rd day in group A. The TGF-beta1 expression increased after operation in both groups, but it decreased in group B on the 3rd day and re-increased after that. The TGF-beta1 expression was significantly different between the two groups on the 7th day (P < 0.05). The expression of collagen I and III decreased during healing. The expression of collagen III in group A was higher on the 3rd day and was lower on the 7th and 10th day than that in group B, showing a significant difference (P < 0.05).
  
  
  CONCLUSIONS
  PAO1 infection could delay the expression of TGF-beta1 and collagen I, III on wound, which may interfere the healing process of wound.
authors.affiliation: Plastic and Reconstructive Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
authors.forename: Lian-bo
authors.initials: LB
authors.lastname: Zhang
authors.forename: Jian-ning
authors.initials: JN
authors.lastname: Li
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Liu
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D005260:Female; D011552:Pseudomonas Infections / Q000473:pathology*; D011550:Pseudomonas aeruginosa; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation; D013530:Surgical Wound Infection / Q000378:metabolism* / Q000382:microbiology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1