title: Clinical trial for safety evaluation of hyaluronidase as diffusion enhancing adjuvant for infiltration analgesia of skin with lidocaine.
abstract: BACKGROUND
  Infiltration anesthesia of the skin is an analgesic procedure often practiced before minor surgical interventions or punctures. The addition of hyaluronidase is a possible option to improve the effectiveness of the local anesthetic with respect to expansion of effect.
  
  
  OBJECTIVE
  To validate the safety of intracutaneous application of hyaluronidase as a lidocaine adjuvant.
  
  
  METHODS
  The influence of adjuvant hyaluronidase on wound healing was investigated using the suction blister method in a prospective, single-center, placebo-controlled, double-blind, intraindividual comparison study with 20 participants. The target parameters were defined as the time of normalization of transepidermal water loss, hemovascular perfusion, and complete macroscopic epithelization of the wound.
  
  
  RESULTS
  No evidence was found that adjuvant application of hyaluronidase retards wound healing.
  
  
  CONCLUSIONS
  The addition of hyaluronidase to lidocaine in intracutaneous infiltration analgesia does not lead to retardation of wound healing, and no additional relevant risks were observed.
authors.affiliation: Department of Dermatology and Venereology, Martin Luther University Halle-Wittenberg, Ernst-Kromayer-Strasse 5, D-06097 Halle (Saale), Germany. johannes.wohlrab@medizin.uni-halle.de
authors.forename: Johannes
authors.initials: J
authors.lastname: Wohlrab
authors.forename: Rainer
authors.initials: R
authors.lastname: Finke
authors.forename: Walter G
authors.initials: WG
authors.lastname: Franke
authors.forename: Angelika
authors.initials: A
authors.lastname: Wohlrab
mesh_terms: D000277:Adjuvants, Pharmaceutic / Q000008:administration & dosage*; D000328:Adult; D000772:Anesthesia, Local / Q000379:methods; D000779:Anesthetics, Local / Q000008:administration & dosage*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage*; D007271:Injections, Intradermal; D008012:Lidocaine / Q000008:administration & dosage*; D008297:Male; D011446:Prospective Studies; D012867:Skin / Q000187:drug effects / Q000503:physiopathology; D014945:Wound Healing / Q000187:drug effects*; D055815:Young Adult
chemical_list: D000277:Adjuvants, Pharmaceutic; D000779:Anesthetics, Local; D008012:Lidocaine; D006821:Hyaluronoglucosaminidase