title: The cathelicidin-like peptide derived from panda genome is a potential antimicrobial peptide.
abstract: A novel cathelicidin-like antimicrobial peptide was identified by mining genome of panda. This peptide (cathelicidin-AM) was synthesized. It showed potential antimicrobial activities against wide spectrum of microorganisms including Gram-negative and -positive bacteria, and fungi. It had similar antimicrobial abilities against both standard and clinically isolated drug-resistant strains. Cathelicidin-AM could rapidly exert its antibacterial activities. It just took less than 1h to kill all Staphylococcus sciuri at the concentration of 2, 4 or 10 times of minimal inhibitory concentration (MIC) while clindamycin took 6h. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis indicated that cathelicidin-AM killed bacteria by directly affecting bacterial cell wall and membrane. Phylogenetic analysis revealed that the panda cathelicidin had the nearest evolution relationship with dog cathelicidin. The current work provides a novel cathelicidin-like peptide with strong antimicrobial abilities.
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Cunbao
authors.initials: C
authors.lastname: Liu
authors.forename: Keyun
authors.initials: K
authors.lastname: Zhang
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D010455:Peptides; D010802:Phylogeny; D016415:Sequence Alignment; D001503:Ursidae / Q000378:metabolism*; D054804:Cathelicidins
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; D054804:Cathelicidins