title: Genetic analysis of the pnp-deaD genetic region reveals membrane lipoprotein NlpI as an independent participant in cold acclimatization of Salmonella enterica serovar Typhimurium.
abstract: The cold acclimatization response in many bacterial species is a tightly regulated process, which ensures the correct folding of macromolecules. In enterobacteria, this response is in part dependent on polynucleotide phosphorylase, which is encoded by the gene pnp. Based on transcriptional analysis of the pnp locus of Salmonella enterica serovar Typhimurium, we show that pnp and the adjacent membrane lipoprotein nlpI gene form an operon with both genes contributing independently to the cold acclimatization response at 15 °C. Our findings thereby define a new role for NlpI in bacterial cold acclimatization.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Syed Fazle
authors.initials: SF
authors.lastname: Rouf
authors.forename: Naeem
authors.initials: N
authors.lastname: Anwar
authors.forename: Mark O
authors.initials: MO
authors.lastname: Clements
authors.forename: Mikael
authors.initials: M
authors.lastname: Rhen
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003080:Cold Temperature; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins