title: Photon-regulated DNA-enzymatic nanostructures by molecular assembly.
abstract: Future smart nanostructures will have to rely on molecular assembly for unique or advanced desired functions. For example, the evolved ribosome in nature is one example of functional self-assembly of nucleic acids and proteins employed in nature to perform specific tasks. Artificial self-assembled nanodevices have also been developed to mimic key biofunctions, and various nucleic acid- and protein-based functional nanoassemblies have been reported. However, functionally regulating these nanostructures is still a major challenge. Here we report a general approach to fine-tune the catalytic function of DNA-enzymatic nanosized assemblies by taking advantage of the trans-cis isomerization of azobenzene molecules. To the best of our knowledge, this is the first study to precisely modulate the structures and functions of an enzymatic assembly based on light-induced DNA scaffold switching. Via photocontrolled DNA conformational switching, the proximity of multiple enzyme catalytic centers can be adjusted, as well as the catalytic efficiency of cofactor-mediated DNAzymes. We expect that this approach will lead to the advancement of DNA-enzymatic functional nanostructures in future biomedical and analytical applications.
authors.affiliation: Department of Chemistry and Physiology and Functional Genomics, Shands Cancer Center, University of Florida, Gainesville, Florida 32611-7200, United States.
authors.forename: Mingxu
authors.initials: M
authors.lastname: You
authors.forename: Ruo-Wen
authors.initials: RW
authors.lastname: Wang
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Kelong
authors.initials: K
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Peng
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D021881:DNA, Catalytic / Q000737:chemistry* / Q000528:radiation effects*; D008027:Light; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry / Q000528:radiation effects* / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D017785:Photons
chemical_list: D021881:DNA, Catalytic; D004247:DNA
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