title: Cloning, expression, purification, crystallization and preliminary X-ray studies of the C-terminal domain of Rv3262 (FbiB) from Mycobacterium tuberculosis.
abstract: During cofactor F(420) biosynthesis, the enzyme F(420)-γ-glutamyl ligase (FbiB) catalyzes the addition of γ-linked L-glutamate residues to form polyglutamylated F(420) derivatives. In Mycobacterium tuberculosis, Rv3262 (FbiB) consists of two domains: an N-terminal domain from the F(420) ligase superfamily and a C-terminal domain with sequence similarity to nitro-FMN reductase superfamily proteins. To characterize the role of the C-terminal domain of FbiB in polyglutamyl ligation, it has been purified and crystallized in an apo form. The crystals diffracted to 2.0 Å resolution using a synchrotron source and belonged to the tetragonal space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 136.6, c = 101.7 Å, α = β = γ = 90°.
authors.affiliation: Structural Biology Laboratory, School of Biological Sciences, The University of Auckland, Auckland, New Zealand. rais002@aucklanduni.ac.nz
authors.forename: Aisyah M
authors.initials: AM
authors.lastname: Rehan
authors.forename: Ghader
authors.initials: G
authors.lastname: Bashiri
authors.forename: Neil G
authors.initials: NG
authors.lastname: Paterson
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Squire
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D010453:Peptide Synthases / Q000737:chemistry*
chemical_list: D010453:Peptide Synthases
references.citation: Nat Struct Biol. 1995 Dec;2(12):1109-14
references.pmid: 8846223
references.citation: Tuber Lung Dis. 1999;79(4):191-8
references.pmid: 10692986
references.citation: Mol Microbiol. 2010 Sep;77(5):1172-85
references.pmid: 20624223
references.citation: J Mol Biol. 2007 Sep 14;372(2):456-69
references.pmid: 17669425
references.citation: PLoS One. 2010 Dec 29;5(12):e15803
references.pmid: 21209917
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
references.pmid: 20124692
references.citation: J Biol Chem. 2008 Jun 20;283(25):17531-41
references.pmid: 18434308
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: Protein Expr Purif. 2005 May;41(1):207-34
references.pmid: 15915565
references.citation: Nat Rev Microbiol. 2005 Jan;3(1):70-80
references.pmid: 15608701
references.citation: Biochem Biophys Res Commun. 2007 Apr 20;355(4):919-25
references.pmid: 17331467
references.citation: Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9785-90
references.pmid: 12909715
references.citation: Arch Microbiol. 2001 Jul;176(1-2):37-43
references.pmid: 11479701
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
references.pmid: 16369096
references.citation: J Bacteriol. 2010 Nov;192(21):5788-98
references.pmid: 20675471
references.citation: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6333-8
references.pmid: 19325122
references.citation: Protein Expr Purif. 2008 Jan;57(1):81-7
references.pmid: 17949993
references.citation: Biochemistry. 2003 Aug 19;42(32):9771-8
references.pmid: 12911320
references.citation: Biosci Biotechnol Biochem. 2004 Jun;68(6):1345-52
references.pmid: 15215601
references.citation: J Bacteriol. 2003 Aug;185(15):4662-5
references.pmid: 12867481
references.citation: Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1378-85
references.pmid: 16204890
references.citation: Mol Microbiol. 1995 Jun;16(6):1137-56
references.pmid: 8577249
references.citation: J Synchrotron Radiat. 2002 Nov 1;9(Pt 6):401-6
references.pmid: 12409628
references.citation: Protein Expr Purif. 2007 Jul;54(1):38-44
references.pmid: 17376702
references.citation: J Bacteriol. 2002 May;184(9):2420-8
references.pmid: 11948155
references.citation: J Biol Chem. 1990 May 15;265(14):8009-15
references.pmid: 2110564
references.citation: J Bacteriol. 2001 Dec;183(24):7058-66
references.pmid: 11717263