title: Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.
abstract: We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.
authors.affiliation: Department of Biology, Washington University in Saint Louis, Saint Louis, MO 63130, USA.
authors.forename: Grigory
authors.initials: G
authors.lastname: Maksaev
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Haswell
mesh_terms: D000818:Animals; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015640:Ion Channel Gating; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008564:Membrane Potentials; D018613:Microscopy, Confocal; D009865:Oocytes / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D029968:Escherichia coli Proteins; D007473:Ion Channels; C469542:MscS protein, E coli
references.citation: Nat Struct Mol Biol. 2005 Feb;12(2):113-9
references.pmid: 15665866
references.citation: Biophys J. 2008 Apr 15;94(8):3003-13
references.pmid: 18065458
references.citation: Pflugers Arch. 1986 Dec;407(6):577-88
references.pmid: 2432468
references.citation: J Gen Physiol. 1972 Nov;60(5):588-608
references.pmid: 4644327
references.citation: J Physiol. 2007 May 15;581(Pt 1):369-87
references.pmid: 17255168
references.citation: Curr Biol. 2006 Jan 10;16(1):1-11
references.pmid: 16401419
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11652-7
references.pmid: 8876191
references.citation: Biophys J. 2006 Oct 15;91(8):2874-81
references.pmid: 16861270
references.citation: Annu Rev Microbiol. 2010;64:313-29
references.pmid: 20825352
references.citation: Proc Natl Acad Sci U S A. 1987 Apr;84(8):2297-301
references.pmid: 2436228
references.citation: J Biol Chem. 2011 Jan 7;286(1):877-88
references.pmid: 20978126
references.citation: Anesth Analg. 2010 Jan 1;110(1):59-63
references.pmid: 19933531
references.citation: J Gen Physiol. 2008 Jul;132(1):67-83
references.pmid: 18591417
references.citation: Mol Nutr Food Res. 2005 Mar;49(3):228-34
references.pmid: 15704243
references.citation: Nat Struct Mol Biol. 2010 Nov;17(11):1330-6
references.pmid: 21037567
references.citation: Nat Struct Mol Biol. 2010 Apr;17(4):451-8
references.pmid: 20208543
references.citation: J Gen Physiol. 2005 Feb;125(2):143-54
references.pmid: 15657299
references.citation: Biochim Biophys Acta. 2000 May 1;1465(1-2):343-58
references.pmid: 10748264
references.citation: Biophys J. 2005 Apr;88(4):3050-9
references.pmid: 15665126
references.citation: J Gen Physiol. 2004 Aug;124(2):151-61
references.pmid: 15277576
references.citation: J Gen Physiol. 1971 Oct;58(4):413-37
references.pmid: 5112659
references.citation: J Gen Physiol. 2010 Jun;135(6):641-52
references.pmid: 20513760
references.citation: Nature. 2007 Jan 4;445(7123):116-9
references.pmid: 17167423
references.citation: Biochem Soc Trans. 2011 Jun;39(3):733-40
references.pmid: 21599642
references.citation: EMBO J. 1998 Aug 3;17(15):4283-90
references.pmid: 9687497
references.citation: Nature. 2009 May 21;459(7245):379-85
references.pmid: 19458714
references.citation: Eur Biophys J. 2009 Sep;38(7):1013-27
references.pmid: 19424690
references.citation: Methods. 2010 May;51(1):183-9
references.pmid: 19963061
references.citation: J Physiol. 1990 Dec;431:103-22
references.pmid: 1712839
references.citation: Nature. 1971 Sep 17;233(5316):177-82
references.pmid: 4939175
references.citation: Biophys J. 2007 Feb 1;92(3):886-902
references.pmid: 17114233
references.citation: Nat Struct Mol Biol. 2007 Dec;14(12):1141-9
references.pmid: 18037888
references.citation: Methods. 2010 May;51(1):66-74
references.pmid: 20123125
references.citation: Biophys J. 2009 Aug 5;97(3):738-47
references.pmid: 19651032
references.citation: J Biol Chem. 2002 Aug 2;277(31):27682-8
references.pmid: 12015316
references.citation: Annu Rev Biophys. 2010;39:111-37
references.pmid: 20192782
references.citation: Exp Cell Res. 1994 Feb;210(2):260-7
references.pmid: 8299724
references.citation: EMBO J. 1999 Apr 1;18(7):1730-7
references.pmid: 10202137
references.citation: Biophys J. 2002 Jul;83(1):290-8
references.pmid: 12080120
references.citation: Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11919-24
references.pmid: 19581583
references.citation: J Biol Chem. 2010 Aug 27;285(35):27176-27181
references.pmid: 20605796
references.citation: FEBS Lett. 2004 Aug 13;572(1-3):233-7
references.pmid: 15304354
references.citation: J Biol Chem. 2002 Apr 19;277(16):13548-55
references.pmid: 11821433
references.citation: J Phys Condens Matter. 2010 Nov 17;22(45):454120
references.pmid: 21339607
references.citation: Biochim Biophys Acta. 1985 Sep 25;819(1):105-13
references.pmid: 3899175
references.citation: EMBO J. 2002 Oct 15;21(20):5323-30
references.pmid: 12374733
references.citation: J Physiol. 1988 Dec;407:311-28
references.pmid: 2476552
references.citation: J Physiol. 1991 Sep;441:347-66
references.pmid: 1816379
references.citation: Biochemistry. 2007 Jun 12;46(23):6766-73
references.pmid: 17500538
references.citation: J Microbiol. 2010 Jun;48(3):325-30
references.pmid: 20571950
references.citation: Science. 2008 Aug 29;321(5893):1210-4
references.pmid: 18755978
references.citation: Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5883-8
references.pmid: 17389370
references.citation: Nat Rev Drug Discov. 2008 Apr;7(4):358-68
references.pmid: 18356919
references.citation: Trends Cell Biol. 2009 May;19(5):228-35
references.pmid: 19342240
references.citation: Methods Enzymol. 1998;293:504-29
references.pmid: 9711626
references.citation: Nat Cell Biol. 2005 Feb;7(2):179-85
references.pmid: 15665854
references.citation: Science. 2008 Aug 29;321(5893):1179-83
references.pmid: 18755969
references.citation: Microbiol Mol Biol Rev. 2003 Mar;67(1):66-85, table of contents
references.pmid: 12626684
references.citation: Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7462-6
references.pmid: 1380158
references.citation: Science. 2002 Nov 22;298(5598):1582-7
references.pmid: 12446901
references.citation: Comb Chem High Throughput Screen. 2009 Jan;12(1):38-50
references.pmid: 19149490
references.citation: Rev Physiol Biochem Pharmacol. 1983;97:1-67
references.pmid: 6306751