title: Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011.
abstract: A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from otitis externa of a dog. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium haemolyticum (97.2 %), Arcanobacterium hippocoleae (96.5 %) and Arcanobacterium phocae (96.4 %). The presence of the major menaquinone MK-9(H(4)) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile contained the major lipids phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unidentified phospholipid (PL2). Major fatty acids were C(14 : 0), C(16 : 0), C(18 : 0), C(18 : 1)ω9c and C(18 : 2)ω6,9c/anteiso-C(18 : 0) (detected as a summed feature). C(10 : 0) and C(12 : 0) were present in minor amounts. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified in the novel species Arcanobacterium canis sp. nov. The type strain of Arcanobacterium canis is P6775(T) (= CCM 7958(T) = CCUG 61573(T) = CIP 110339(T)). An emended description of the genus Arcanobacterium is also provided.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
authors.forename: Muaz
authors.initials: M
authors.lastname: Hijazin
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Frankfurter Straße 85-91, 35392 Giessen, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
authors.forename: Osama
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Alber
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institute für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: De Gezondheidsdienst voor Dieren (Animal Health Service), Postbus 9, 7400 AA Deventer, The Netherlands.
authors.forename: Abdulwahed Ahmed
authors.initials: AA
authors.lastname: Hassan
authors.affiliation: Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Landesbetrieb Hessisches Landeslabor, Schubert Straße 60, 35392 Giessen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D000818:Animals; D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010032:Otitis Externa / Q000382:microbiology*; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S