title: Pkc1 and actin polymerisation activities play a role in ribosomal gene repression associated with secretion impairment caused by oxidative stress.
abstract: In Saccharomyces cerevisiae, the cell integrity pathway plays a role in the oxidative stress response. In this study, we show that the Pkc1 protein mediates oxidative signalling by helping to downregulate ribosomal gene expression when cells are exposed to hydrogen peroxide. An active actin cytoskeleton is required for this function, because the cells blocked in actin polymerisation were unable to repress ribosomal gene transcription. Following the invertase secretion pattern, we hypothesize that oxidative stress induced by hydrogen peroxide could have affected the latter steps of secretion. This would explain why the Pkc1 function was required to repress ribosomal biogenesis.
authors.affiliation: Prince Felipe Research Centre, Valencia, Spain.
authors.forename: Felipe V
authors.initials: FV
authors.lastname: Mitjana
authors.forename: Mima I
authors.initials: MI
authors.lastname: Petkova
authors.forename: Nuria
authors.initials: N
authors.lastname: Pujol-Carrion
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: de la Torre-Ruiz
mesh_terms: D000199:Actins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D003599:Cytoskeleton / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D058105:Polymerization; D011493:Protein Kinase C / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D055571:Secretory Pathway / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
chemical_list: D000199:Actins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D011493:Protein Kinase C