title: Biomolecular modification of carbon nanotubes for studies of cell adhesion and migration.
abstract: We report a strategy for tailoring and patterning carbon nanotubes (CNTs) for biospecific cell studies. We synthesized a new electroactive hydroquinone terminated pyrene molecule to tailor CNTs. These modified CNTs can be oxidized and chemoselectively reacted with oxyamine tethered ligands to generate various ligand tethered CNTs. A cell adhesive Arg-Gly-Asp peptide (RGD) is immobilized to the CNTs and a new microfluidic patterning method is employed to generate multiplex patterned surfaces for biospecific cell adhesion and migration studies. This work demonstrates the integration of a new functionalization strategy to immobilize a variety of ligands to CNTs for a range of potential drug delivery, tissue imaging and cellular behavior studies and a microfluidic patterning strategy for generating complex high-throughput surfaces for biotechnological and cell based assay applications.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Yousaf
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D055664:Electrochemical Techniques; D005347:Fibroblasts / Q000166:cytology*; D006873:Hydroquinones / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry*; D010084:Oxidation-Reduction; D011721:Pyrenes / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D006873:Hydroquinones; D008024:Ligands; D037742:Nanotubes, Carbon; D009842:Oligopeptides; D011721:Pyrenes; C047981:arginyl-glycyl-aspartic acid; C030984:pyrene; C031927:hydroquinone