title: Transcriptional analysis of equine λ-light chains in the horse breeds Rhenish-German Coldblood and Hanoverian Warmblood.
abstract: The present study analyzed equine λ-light chain genes (IGLV and IGLC) transcribed in the horse breeds Rhenish-German Coldblood (RGC) and Hanoverian Warmblood (HW). Primers were generated for the major expressed IGLV subgroup 8. The significant majority of the sequences represented IGLC6/7. In RGC, IGLC1 and IGLC5 were observed in significant higher frequencies than IGLC4. In HW, significant differences were obtained for the transcription of IGLC1 and IGLC5. IGLC4 was not determined in this breed. Five allotypic IGLC1 variants, four allotypic IGLC5 variants, and three allelic as well as two allotypic IGLC6/7 variants were identified. IGLC1(b, d), IGLC5(c, d), and IGLC6/7(a3, b) were detected in RGC while IGLC1(c) and IGLC5(b) were solely found in HW. Furthermore, 11 out of 144 known IGLV-segments were transcribed of which IGLV15 and IGLV17 were preferred significantly. IGLV25 displayed significant differences in the rearrangement between both breeds. The classified pseudogenes IGLV101ψ and IGLV74ψ were also identified. Rearrangements with IGLC-genes showed significant differences for IGLV15 in both breeds, whereas IGLV25 also revealed significant differences between the breeds. The transcriptional orientation of the functional segments has no influence on the occurrence of the IGLV.
authors.affiliation: Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August University Göttingen Burckhardtweg 2, 37077 Göttingen, Germany. stefanie.hara@t-online.de
authors.forename: Stefanie
authors.initials: S
authors.lastname: Hara
authors.forename: Ulrike S
authors.initials: US
authors.lastname: Diesterbeck
authors.forename: Sven
authors.initials: S
authors.lastname: König
authors.forename: Claus-Peter
authors.initials: CP
authors.lastname: Czerny
mesh_terms: D000483:Alleles; D000818:Animals; D050439:Genes, Immunoglobulin Light Chain / Q000235:genetics; D006736:Horses / Q000235:genetics* / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000235:genetics / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000235:genetics / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity
chemical_list: D007126:Immunoglobulin Allotypes; D007127:Immunoglobulin Constant Regions; D007146:Immunoglobulin lambda-Chains