title: [The Enhanceing effect of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils in tuberculosis patients].
abstract: OBJECTIVE
  To explore the effects of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils or polymorphonuclear cells (PMNs)  in tuberculosis patients.
  
  
  METHODS
  The fresh peripheral blood samples from TB patients and healthy adults were incubated with M.tb labeled with FITC,  and the percentages of phagocytosis of M.tb by PMNs was measured by flow cytometry (FCM). The fresh peripheral blood samples were incubated with DCFH-DA,  and with or without M.tb for different times,  the percentage of activation and the ROS production of PMNs were measured by FCM. Whole blood samples were pretreated with IL-12,  the changes of phagocytosis,  activation and ROS production of PMNs were measured by FCM.
  
  
  RESULTS
  The percentages of phagocytosis by PMNs,  activation and ROS production of PMNs in both TB patients and healthy adults increased dependent on the time of incubation with M.tb. Only the phagocytosis of M.tb by PMNs at 5 min in TB patients of tuberculosis patients (51.82±6.93)% was obviously higher than that in healthy adults (47.20±4.26)%,  (P<0.05). Pretreatment of whole blood with IL-12 before incubation with M.tb,  the percentages of phagocytosis,  activation and ROS production of PMNs in both TB patients and healthy adults increased in dose dependent manner,  but no significant difference was found between both groups.
  
  
  CONCLUSIONS
  The results indicated that the phagocytosis of M.tb and ROS production by PMNs in TB patients were almost the same as that in healthy controls,  except for phagocytosis is higher at early stage. Furthermore,  IL-12 can enhance the responsiveness to the phagocytosis and ROS production of PMNs.
authors.affiliation: Department of Immunology, Bengbu Medical College, Bengbu, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Jiang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Yao
authors.forename: Qi-li
authors.initials: QL
authors.lastname: Jin
authors.forename: Wen-xin
authors.initials: WX
authors.lastname: He
authors.forename: Bai-qing
authors.initials: BQ
authors.lastname: Li
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; D018664:Interleukin-12