title: Increased expression of placental growth factor in patients with temporal lobe epilepsy and a rat model.
abstract: Placental growth factor (PIGF) plays a role in angiogenesis and neuroprotection. It has been suggested that angiogenesis and blood-brain barrier damage are involved in the pathophysiology of epilepsy. In this study, we investigated the PIGF expression in the temporal neocortices of 11 patients with pharmaco-resistant temporal lobe epilepsy (TLE) and 6 non-epileptic controls, using double immunofluorescence labeling, immunohistochemistry and Western blotting. We also assessed PIGF expression pattern in a rat model of TLE induced by lithium chloride-pilocarpine. We found that PIGF expression was significantly elevated in patients with TLE than in control. TLE patients with initial injuries had significantly higher PIGF level than those without initial injuries. In the TLE rat model, PIGF upregulation started at 6h after status epilepticus and maintained at significant high level for up to 60 days. These results suggest that the augmentation of brain PIGF is associated with development of epilepsy.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1st Youyi Road, Chongqing 400016, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.forename: Zonggang
authors.initials: Z
authors.lastname: Yue
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhou
authors.forename: Fenghua
authors.initials: F
authors.lastname: Zhao
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Guojun
authors.initials: G
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D002648:Child; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C000608036:PGF protein, human; C000608038:Pgf protein, rat; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor