title: Decreased expression of V-set and immunoglobulin domain containing 1 (VSIG1) is associated with poor prognosis in primary gastric cancer.
abstract: BACKGROUND
  To date, the significance of altered expression of V-set and immunoglobulin domain containing 1 (VSIG1) in gastric cancer has not yet been elucidated.
  
  
  METHODS
  We examined VSIG1 expression in 30 paired gastric cancer tissues and noncancerous gastric mucosa as well as in 5 gastric cancer cell lines by real-time PCR and Western blotting. In addition, we analyzed VSIG1 expression in 232 gastric adenocarcinoma samples by immunohistochemistry.
  
  
  RESULTS
  VSIG1 expression was significantly reduced at both the mRNA and protein levels in gastric cancer tissues. Immunohistochemistry revealed that VSIG1 expression was completely lost in 126 out of the 232 (54.3%) patient samples and remarkably reduced in another 106 (45.7%) patients. Negative VSIG1 expression was significantly correlated with tumor size (P = 0.007), T (P = 0.023), and M stage (P = 0.037). Importantly, loss of VSIG1 expression was significantly correlated with poor overall survival (OS, P < 0.001) and disease-free survival (DFS, P = 0.006) in gastric cancer patients. Cox regression analyses showed that VSIG1 expression was an independent predictor of OS (P = 0.002) and DFS (P = 0.039).
  
  
  CONCLUSIONS
  Our findings suggest that silencing VSIG1 may play an important role in gastric carcinogenesis and that VSIG1 may serve as a prognostic marker as well as a potential therapeutic target for gastric cancer.
authors.affiliation: State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat-Sen University Cancer Center, Guangzhou, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Pan
authors.forename: Shengping
authors.initials: S
authors.lastname: Li
authors.forename: Jianchuan
authors.initials: J
authors.lastname: Xia
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jugao
authors.initials: J
authors.lastname: Chen
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Lü
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Qiuzhong
authors.initials: Q
authors.lastname: Pan
authors.forename: Qijing
authors.initials: Q
authors.lastname: Wang
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C510166:VSIG1 protein, human