title: Stimulation of the endometrium with high-grade blastocyst culture supernatant (SEHB) can improve pregnancy outcome for couples undergoing intracytoplasmic sperm injection (ICSI): a randomized clinical trial.
abstract: OBJECTIVE
  To evaluate the impact of stimulating the endometrium with high-grade blastocyst culture supernatant (SEHB) perfusion before blastocyst transfer (BT) on implantation rate, pregnancy rate, and pregnancy outcome in ICSI cycles.
  
  
  METHODS
  Ninety-four infertile couples who were referred to the Valiasr department of Imam Hospital complex between January 2010 and March 2011 enrolled in this randomized clinical trial. They were randomly divided into only BT or SEHB groups. Implantation rates, pregnancy rates, abortion, preterm and term delivery rates were compared between the two groups.
  
  
  RESULTS
  Implantation rates and pregnancy rates did not differ significantly between the two groups. The abortion rate was significantly higher in the BT only group while term delivery was significantly higher in SEHB group. Odds ratios of term delivery and abortion were 4.5 (p = 0.001) and 0.3 (p = 0.04), respectively.
  
  
  CONCLUSIONS
  The SEHB application may improve pregnancy outcome in infertile couples who are candidates for the ICSI reproductive method.
authors.affiliation: Department of Endocrinology and Female Infertility, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Ensieh Shahrokhe
authors.initials: ES
authors.lastname: Tehraninejad
authors.forename: Fateme Davari
authors.initials: FD
authors.lastname: Tanha
authors.forename: Mahsa
authors.initials: M
authors.lastname: Ghajarzadeh
authors.forename: Zahra
authors.initials: Z
authors.lastname: Zandieh
authors.forename: Elham
authors.initials: E
authors.lastname: Aziminekoo
authors.forename: Haleh Rahmanpour
authors.initials: HR
authors.lastname: Zanjani
mesh_terms: D000328:Adult; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods*; D004717:Endometrium / Q000502:physiology; D005260:Female; D006801:Humans; D007246:Infertility / Q000628:therapy*; D008297:Male; D010062:Ovulation Induction; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic