title: Immune responses of chickens inoculated with a recombinant fowlpox vaccine coexpressing glycoprotein B of infectious laryngotracheitis virus and chicken IL-18.
abstract: Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes severe and economically significant respiratory disease in poultry worldwide. Herein, the immunogenicity of two recombinant fowlpox viruses (rFPV-gB and rFPV-gB/IL18) containing ILTV glycoprotein B (gB) and chicken interleukin-18 (IL-18) were investigated in a challenge model. One-day-old specific-pathogen-free chickens were vaccinated by wing-web puncture with the two rFPVs and challenged with the virulent ILTV CG strain. There were differences in antibody levels elicited by either rFPV-gB/IL18 or rFPV-gB as determined using ELISA. The ratios of CD4(+) to CD8(+) in chickens immunized with rFPV-gB/IL18 were higher (P < 0.05) than in those immunized with rFPV-gB, and the level of proliferative response of the T cells in the rFPV-gB/IL18-vaccinated group was higher (P < 0.05) than that in the rFPV-gB group. All chickens immunized with rFPV-gB/IL18 were protected (10/10), whereas only eight of 10 of the chickens immunized with the rFPV-gB were protected. The results showed that the protective efficacy of the rFPV-gB vaccine could be enhanced by simultaneous expression of chicken IL-18.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Pei
authors.initials: P
authors.lastname: Cui
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Cui
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Li
authors.forename: Xian-Qin
authors.initials: XQ
authors.lastname: Jiao
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Zheng
authors.forename: Guo
authors.initials: G
authors.lastname: Cheng
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Chao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016516:CD4-CD8 Ratio; D049109:Cell Proliferation; D002645:Chickens; D004337:Drug Carriers / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D005587:Fowlpox virus / Q000235:genetics*; D005822:Genetic Vectors / Q000008:administration & dosage*; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D001352:Herpesvirus 1, Gallid / Q000235:genetics / Q000276:immunology*; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D020382:Interleukin-18; D014614:Vaccines, Synthetic; D014765:Viral Vaccines