title: [Simultaneous determination of six flavonoids in Hyperici Japonici Herba by HPCE-DAD].
abstract: OBJECTIVE
  To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin, isoquercitrin, hyperoside, quercitrin, kaempferol and quercetin in Hyperici Japonici Herba.
  
  
  METHODS
  Based on the mode of capillary zone electrophoresis, 40 mmol x L(-1) borax was used as buffer solution (pH 8.62), uncoated fused silica capillary (56 cm x 64.5 cm x 75 microm) was used, separation voltage was 25 kV, detection wavelength was at 206 nm, column temperature was maintained at 25 degrees C, and sample was injected at 50 mbar, 8 s.
  
  
  RESULTS
  Six flavonoids showed good linearity (r > 0.9953) in the range of the tested concentration, the average recoveries of the method were between 98.8%-102.9%.
  
  
  CONCLUSIONS
  The method is simple, accurate and reproducible, and can be used for quality control of Hyperici Japonici Herba.
authors.affiliation: Pharmnacology College of Nanjing University of Chinese Medicine, Nanjing 210046, China. winner2243@sohu.com
authors.forename: Ke
authors.initials: K
authors.lastname: Han
authors.forename: Xunhang
authors.initials: X
authors.lastname: Liu
authors.forename: Qinan
authors.initials: Q
authors.lastname: Wu
authors.forename: Junsong
authors.initials: J
authors.lastname: Li
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Xingsheng
authors.initials: X
authors.lastname: Fu
authors.forename: Aiping
authors.initials: A
authors.lastname: Lu
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D020902:Hypericum / Q000737:chemistry*; D013696:Temperature
chemical_list: D005419:Flavonoids