title: Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae.
abstract: Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae, and supplementation of fermentation with several amino acids can increase intracellular GSH content. More recently, however, focus has been given to protein as a resource for biofuel and fine chemical production. We demonstrate that expression of a protease on the cell surface of S. cerevisiae enables the direct use of keratin and soy protein as a source of amino acids and that these substrates enhanced intracellular GSH content. Furthermore, fermentation using soy protein also enhanced cell concentration. GSH fermentation from keratin and to a greater extent from soy protein using protease-displaying yeast yielded greater GSH productivity compared to GSH fermentation with amino acid supplementation. This protease-displaying yeast is potentially applicable to a variety of processes for the bio-production of value-added chemicals from proteinaceous biomass resources.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Nada-ku, Kobe, Japan.
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Songhee
authors.initials: S
authors.lastname: Kim
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Naoko
authors.initials: N
authors.lastname: Okai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D018533:Biomass; D005285:Fermentation; D015870:Gene Expression; D005978:Glutathione / Q000302:isolation & purification* / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D060847:Metabolic Engineering; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D059748:Proteolysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D030262:Soybean Proteins; D007633:Keratins; D010447:Peptide Hydrolases; D005978:Glutathione