title: Investigation of six selected bacterial species in endo-periodontal lesions.
abstract: OBJECTIVE
  To investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: Parvimonas micra, Fusobacterium nucleatum, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens and Capnocytophaga sputigena.
  
  
  METHODS
  Forty-six patients presenting with different types of endo-periodontal lesions were investigated. Clinical examinations, periapical radiographs and microbiological sampling from the canal system (endo) and periodontal pockets (perio) were performed. Qualitative and semiquantitative evaluation of bacteria was performed by polymerase chain reaction (PCR) and DNA-DNA hybridization (micro-IDent plus; Hain Lifescience, Germany).
  
  
  RESULTS
  Extremely high bacterial loads in endodontic samples were recorded for P. micra, F. nucleatum and C. sputigena, while periodontal samples were often colonized by the same species, plus C. rectus. Significant association was recorded between F. nucleatum-endo and P. micra-endo (P = 0.03, Fisher's exact test). There was marginal evidence of associations between: (i) C. sputigena-endo and C. sputigena-perio (P = 0.06, Fisher's exact test); (ii) P. micra-endo and P. micra-perio (P = 0.05, Fisher's exact test). Sensitivity to percussion was associated with an increased chance of cases with P. micra-endo (P = 0.03, Pearson chi-square test).
  
  
  CONCLUSIONS
  The findings suggest that F. nucleatum, P. micra and C. sputigena may play a role in the pathogenesis of endo-periodontal lesions.
authors.affiliation: Carol Davila University of Medicine and Pharmacy, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania. Andreea.Didilescu@gmail.com
authors.forename: A C
authors.initials: AC
authors.lastname: Didilescu
authors.forename: D
authors.initials: D
authors.lastname: Rusu
authors.forename: A
authors.initials: A
authors.lastname: Anghel
authors.forename: L
authors.initials: L
authors.lastname: Nica
authors.forename: A
authors.initials: A
authors.lastname: Iliescu
authors.forename: M
authors.initials: M
authors.lastname: Greabu
authors.forename: G
authors.initials: G
authors.lastname: Bancescu
authors.forename: S I
authors.initials: SI
authors.lastname: Stratul
mesh_terms: D016301:Alveolar Bone Loss / Q000382:microbiology; D058491:Bacterial Load; D044883:Campylobacter rectus / Q000302:isolation & purification; D002206:Capnocytophaga / Q000302:isolation & purification; D055113:Chronic Periodontitis / Q000382:microbiology; D003774:Dental Plaque Index; D003786:Dental Pulp Cavity / Q000382:microbiology; D003788:Dental Pulp Diseases / Q000382:microbiology*; D003790:Dental Pulp Necrosis / Q000382:microbiology; D016124:Eikenella corrodens / Q000302:isolation & purification; D005051:Eubacterium / Q000302:isolation & purification; D005674:Fusobacterium Infections / Q000175:diagnosis; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D005884:Gingival Hemorrhage / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010462:Peptostreptococcus / Q000302:isolation & purification; D010485:Periapical Periodontitis / Q000382:microbiology; D017622:Periodontal Attachment Loss / Q000382:microbiology; D010510:Periodontal Diseases / Q000382:microbiology*; D010514:Periodontal Pocket / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016300:Radiography, Bitewing; D014086:Tooth Mobility / Q000382:microbiology; D019553:Tooth, Nonvital / Q000382:microbiology