title: Small interfering RNA targeting Krüppel-like factor 8 inhibits U251             glioblastoma cell growth by inducing apoptosis.
abstract: Small interfering RNAs (siRNAs) are small non-coding RNAs, comprising 21-23 nucleotides that regulate gene expression by transcriptionally repressing their complementary mRNAs. In particular, Krüppel-like factor 8 (KLF8) has been postulated to function as a tumor activator in various cancer cells, but not in glioblastoma. In the present study, we investigated the anti-tumorigenic effect of KLF8 siRNA in glioblastoma cells. U251 human glioblastoma cells were transfected with KLF8 siRNA and assayed for in vitro proliferation, cell cycle and cell apoptosis. The transfection of KLF8 siRNA reduced expression of KLF8 in the glioblastoma cells. KLF8 siRNA also reduced in vitro proliferation and enhanced cell apoptosis. These results indicate that KLF8 siRNA has an anti-tumorigenic effect on glioblastoma cells and suggest the possible use of KLF8 siRNA for the treatment of glioblastoma.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wan
authors.forename: Ji
authors.initials: J
authors.lastname: Zhu
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Sun
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Tang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016195:G2 Phase; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering / Q000378:metabolism*; D012097:Repressor Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D012097:Repressor Proteins