title: Alteration of seed storage protein composition in soybean [Glycine max (L.) Merrill] mutant lines induced by γ-irradiation mutagenesis.
abstract: This study investigated the alteration of seed storage proteins in soybean mutants induced by γ-irradiation. Five soybean cultivars and four landraces were irradiated with 250 Gy of γ rays to induce variability. The seed storage protein profiles of 414 genetic fixed mutants (M(12)-M(20)) having excellent agricultural traits were analyzed by SDS-PAGE. Among the 414 mutants, 58 were identifed as lacking lipoxygenase, 89 lacking the α' subunit, 113 lacking the α subunit, and 40 with an altered β subunit. One hundred and forty-nine mutants lacked the A(3) subunit of glycinin. Fifty-four mutants showed higher trypsin inhibitor (TIA) activity, whereas 139 showed lower TIA activity compared to their original cultivars. The selected mutants with low amounts of antinutritional factors such as trypsin inhibitor, lipoxygenase, and α subunit will constitute genetic resources for improving soybean protein quality.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong, Jeongeup 580-185, Republic of Korea.
authors.forename: Kyung Jun
authors.initials: KJ
authors.lastname: Lee
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Bo-Keun
authors.initials: BK
authors.lastname: Ha
authors.forename: Byung-Moo
authors.initials: BM
authors.lastname: Lee
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Kang
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Kim
mesh_terms: D005720:Gamma Rays; D016296:Mutagenesis; D055314:Seed Storage Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000235:genetics*; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max / Q000235:genetics* / Q000528:radiation effects; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis
chemical_list: D055314:Seed Storage Proteins; D030262:Soybean Proteins; D014360:Trypsin Inhibitor, Kunitz Soybean