title: A wide diversity of cortical GABAergic interneurons derives from the embryonic preoptic area.
abstract: GABA-containing (GABAergic) interneurons comprise a very heterogeneous group of cells that are crucial for cortical function. Different classes of interneurons specialize in targeting specific subcellular domains of excitatory pyramidal cells or other interneurons, which provides cortical circuits with an enormous capability for information processing. As in other regions of the CNS, cortical interneuron diversity is thought to emerge from the genetic specification of different groups of progenitor cells within the subpallium. Most cortical interneurons originate from two main regions, the medial and the caudal ganglionic eminences (MGE and CGE, respectively). In addition, it has been shown that progenitors in the embryonic preoptic area (POA) also produce a small population of cortical GABAergic interneurons. Here, we show that the contribution of the POA to the complement of cortical GABAergic interneurons is larger than previously believed. Using genetic fate mapping and in utero transplantation experiments, we demonstrate that Dbx1-expressing progenitor cells in the POA give rise to a small but highly diverse cohort of cortical interneurons, with some neurochemical and electrophysiological characteristics that were previously attributed to MGE- or CGE-derived interneurons. There are, however, some features that seem to distinguish POA-derived interneurons from MGE- or CGE-derived cells, such as their preferential laminar location. These results indicate that the mechanisms controlling the specification of different classes of cortical interneurons might be more complex than previously expected. Together with earlier findings, our results also suggest that the POA generates nearly 10% of the GABAergic interneurons in the cerebral cortex of the mouse.
authors.affiliation: Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
authors.forename: Diego
authors.initials: D
authors.lastname: Gelman
authors.forename: Amélie
authors.initials: A
authors.lastname: Griveau
authors.forename: Nathalie
authors.initials: N
authors.lastname: Dehorter
authors.forename: Anne
authors.initials: A
authors.lastname: Teissier
authors.forename: Carolina
authors.initials: C
authors.lastname: Varela
authors.forename: Ramón
authors.initials: R
authors.lastname: Pla
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pierani
authors.forename: Oscar
authors.initials: O
authors.lastname: Marín
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins / Q000235:genetics; D001973:Bromodeoxyuridine / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D004558:Electric Stimulation; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D066298:In Vitro Techniques; D007211:Indoles / Q000378:metabolism; D007395:Interneurons / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D018408:Patch-Clamp Techniques; D011301:Preoptic Area / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D013003:Somatosensory Cortex / Q000166:cytology* / Q000254:growth & development; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C103403:Dbx1 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D018398:Homeodomain Proteins; D007211:Indoles; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D022661:RNA, Untranslated; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; C007293:DAPI; D005680:gamma-Aminobutyric Acid; D001973:Bromodeoxyuridine
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