title: Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis.
abstract: A pinkish-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium, strain E92(T), was isolated from the skin of the medical leech Hirudo medicinalis, on R2A agar. 16S rRNA gene sequence analysis of strain E92(T) showed a relatively low 16S rRNA gene sequence similarity (93.0-93.5 %) to representatives of the genus Arcicella and 91.5-92.0 % to members of the genus Flectobacillus. The polar lipid profile was composed of the major compounds phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified aminolipid and an unidentified polar lipid; glycolipids were not detected. The major quinone was menquinone MK-7, and the major compound in the polyamine pattern was spermidine. The predominant fatty acids were C(16 : 1)ω5c and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH (detected as summed feature 3). The isolate did not contain C(14 : 0) or the hydroxyl fatty acid iso-C(17 : 0) 3-OH found in all representatives of the genera Arcicella and Flectobacillus, but did produce C(18 : 1)ω7c and 11-methyl C(18 : 1)ω7c which are not found in these two genera. The DNA G+C content of strain E92(T) was 64.4 mol%. The unique 16S rRNA gene sequence, and specific chemotaxonomic and physiological data revealed that strain E92(T) represents a new genus in the family Cytophagaceae for which we propose the name Pseudarcicella hirudinis gen. nov., sp. nov., with the type strain of the type species as E92(T) (= LMG 26720(T) = CCM 7988(T)).
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Igor
authors.initials: I
authors.lastname: Longaric
authors.affiliation: Marine Microbiology Group, Institut Mediterrani d'Estudis Avançats (CSIC-UIB), C/Miquel Marqués 21, 07190 Esporles, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Rosselló-Móra
authors.affiliation: Biebertaler Blutegelzucht, D-35444 Biebertal, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Galatis
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Lodders
mesh_terms: D000818:Animals; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D047709:Hirudo medicinalis / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7