title: Identification of cortical lamination in awake monkeys by high resolution magnetic resonance imaging.
abstract: Brodmann divided the neocortex into 47 different cortical areas based on histological differences in laminar myeloarchitectonic and cytoarchitectonic defined structure. The ability to do so in vivo with anatomical magnetic resonance (MR) methods in awake subjects would be extremely advantageous for many functional studies. However, due to the limitations of spatial resolution and contrast, this has been difficult to achieve in awake subjects. Here, we report that by using a combination of MR microscopy and novel contrast effects, cortical layers can be delineated in the visual cortex of awake subjects (nonhuman primates) at 4.7 T. We obtained data from 30-min acquisitions at voxel size of 62.5 × 62.5 × 1000 μm(3) (4 nl). Both the phase and magnitude components of the T(2)*-weighted image were used to generate laminar profiles which are believed to reflect variations in myelin and local cell density content across cortical depth. Based on this, we were able to identify six layers characteristic of the striate cortex (V1). These were the stripe of Kaes-Bechterew (in layer II/III), the stripe of Gennari (in layer IV), the inner band of Baillarger (in layer V), as well as three sub-layers within layer IV (IVa, IVb, and IVc). Furthermore, we found that the laminar structure of two extrastriate visual cortex (V2, V4) can also be detected. Following the tradition of Brodmann, this significant improvement in cortical laminar visualization should make it possible to discriminate cortical regions in awake subjects corresponding to differences in myeloarchitecture and cytoarchitecture.
authors.affiliation: Department of Psychology, Vanderbilt University, Nashville, TN 37203, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.forename: Anna W
authors.initials: AW
authors.lastname: Roe
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology*; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging; D014851:Wakefulness / Q000502:physiology*
references.citation: NMR Biomed. 2009 Jun;22(5):480-7
references.pmid: 19123230
references.citation: Cereb Cortex. 1991 Jan-Feb;1(1):1-47
references.pmid: 1822724
references.citation: Prog Neurobiol. 2002 Aug;67(5):393-420
references.pmid: 12234501
references.citation: J Magn Reson Imaging. 2003 Sep;18(3):284-90
references.pmid: 12938122
references.citation: J Comp Neurol. 1996 Feb 05;365(2):232-55
references.pmid: 8822167
references.citation: Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2981-6
references.pmid: 12601170
references.citation: Radiology. 2000 Jan;214(1):217-21
references.pmid: 10644127
references.citation: Magn Reson Imaging. 2008 Sep;26(7):935-42
references.pmid: 18524522
references.citation: Brain Res Bull. 1981 Nov;7(5):519-79
references.pmid: 7317796
references.citation: Hum Brain Mapp. 2005 Dec;26(4):240-50
references.pmid: 15954140
references.citation: Neurotherapeutics. 2007 Jul;4(3):460-84
references.pmid: 17599712
references.citation: Magn Reson Imaging. 2006 May;24(4):381-92
references.pmid: 16677944
references.citation: Neuroimage. 2009 Oct 1;47(4):1252-60
references.pmid: 19520174
references.citation: Magn Reson Imaging. 2010 Oct;28(8):1210-5
references.pmid: 20392587
references.citation: Cereb Cortex. 2011 Sep;21(9):2075-81
references.pmid: 21310782
references.citation: J Neurosci. 1991 Aug;11(8):2622-9
references.pmid: 1714496
references.citation: Neuron. 2010 Dec 9;68(5):1002-13
references.pmid: 21145011
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 10;104(28):11796-801
references.pmid: 17586684
references.citation: Cereb Cortex. 1992 Sep-Oct;2(5):417-24
references.pmid: 1422094
references.citation: Neuroimage. 2008 Feb 15;39(4):1585-99
references.pmid: 18055222
references.citation: Magn Reson Med. 2004 Jan;51(1):22-6
references.pmid: 14705041
references.citation: J Neurocytol. 1974 Nov;3(5):619-29
references.pmid: 4142638
references.citation: J Chem Neuroanat. 2000 Oct;20(1):31-47
references.pmid: 11074342
references.citation: J Comp Neurol. 1981 Oct 1;201(4):519-39
references.pmid: 7287933
references.citation: Neuroimage. 2009 Feb 15;44(4):1259-66
references.pmid: 19027861
references.citation: Neuroimage. 2006 Sep;32(3):1176-84
references.pmid: 16806980
references.citation: Nat Neurosci. 2010 Dec;13(12):1542-8
references.pmid: 21076422
references.citation: Magn Reson Imaging. 2012 Jan;30(1):36-47
references.pmid: 22055855
references.citation: Magn Reson Med. 2002 Oct;48(4):735-8
references.pmid: 12353293
references.citation: Ann N Y Acad Sci. 1992 Mar 31;649:285-301
references.pmid: 1580500
references.citation: Biophys Chem. 1994 May;50(1-2):225-35
references.pmid: 8011936
references.citation: J Cereb Blood Flow Metab. 2007 Jun;27(6):1248-61
references.pmid: 17213863
references.citation: Biochim Biophys Acta. 1982 Feb 2;714(2):265-70
references.pmid: 6275909
references.citation: Magn Reson Imaging. 2007 Jul;25(6):740-7
references.pmid: 17499466
references.citation: Magn Reson Imaging. 2010 Oct;28(8):1183-91
references.pmid: 20456890
references.citation: Hum Brain Mapp. 2005 Mar;24(3):206-15
references.pmid: 15543596
references.citation: Neuroimage. 2006 Oct 1;32(4):1524-37
references.pmid: 16806989
references.citation: Front Hum Neurosci. 2011 Feb 18;5:19
references.pmid: 21373360
references.citation: Magn Reson Med. 2006 Jul;56(1):216-23
references.pmid: 16767762
references.citation: J Neurosci. 1988 Jun;8(6):1831-45
references.pmid: 3385477
references.citation: Magn Reson Med. 2004 Sep;52(3):612-8
references.pmid: 15334582
references.citation: J Vis. 2005 Feb 11;5(2):93-102
references.pmid: 15831070
references.citation: Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3834-9
references.pmid: 20133720
references.citation: J Anat Physiol. 1907 Apr;41(Pt 3):198-207
references.pmid: 17232729
references.citation: Magn Reson Med. 2011 Jul;66(1):24-31
references.pmid: 21574183
references.citation: Magn Reson Med. 1990 Oct;16(1):9-18
references.pmid: 2255240
references.citation: Magn Reson Imaging. 2004 Dec;22(10):1343-59
references.pmid: 15707785