title: Heterogeneity and dynamics of lateral line afferent innervation during development in zebrafish (Danio rerio).
abstract: The lateral line system of larval zebrafish is emerging as a model to study a range of topics in neurobiology, from hair cell regeneration to sensory processing. However, despite numerous studies detailing the patterning and development of lateral line neuromasts, little is known about the organization of their connections to afferent neurons and targets in the hindbrain. We found that as fish grow and neuromasts proliferate over the body surface, the number of afferent neurons increases linearly. The number of afferents innervating certain neuromasts increases over time, while it decreases for other neuromasts. The ratio of afferent neurons to neuromasts differs between the anterior and posterior lateral line system, suggesting potential differences in sensitivity threshold or spatial resolution. A single afferent neuron routinely contacts a group of neuromasts, suggesting that different afferent neurons can convey information about receptive fields along the body. When afferent projections are traced into the hindbrain, where a distinct somatotopy has been previously described, we find that this general organization is absent at the Mauthner cell. We speculate that directional input from the lateral line is less important at an early age, whereas the speed of the escape response is paramount, and that directional responses arise later in development. By quantifying morphological connections in the lateral line system, this study provides a detailed foundation to understand how hydrodynamic information is processed and ultimately translated into appropriate motor behaviors.
authors.affiliation: The Whitney Laboratory for Marine Bioscience, Department of Biology, University of Florida, St. Augustine, Florida 32080, USA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Haehnel
authors.forename: Masashige
authors.initials: M
authors.lastname: Taguchi
authors.forename: James C
authors.initials: JC
authors.lastname: Liao
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology / Q000254:growth & development; D018274:Electroporation; D007814:Larva; D053403:Lateral Line System / Q000166:cytology* / Q000254:growth & development*; D018613:Microscopy, Confocal; D009434:Neural Pathways / Q000166:cytology* / Q000254:growth & development*; D055495:Neurogenesis / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology; D015027:Zebrafish / Q000254:growth & development*
references.citation: Brain Res. 1975 Oct 17;96(2):349-56
references.pmid: 1175018
references.citation: Can J Neurol Sci. 2003 Aug;30(3):189-200
references.pmid: 12945940
references.citation: Brain Res. 1975 Oct 17;96(2):342-8
references.pmid: 169962
references.citation: J Comp Neurol. 2000 May 29;421(2):189-98
references.pmid: 10813781
references.citation: J Cell Biol. 1962 Oct;15:19-27
references.pmid: 13945569
references.citation: Neuron. 2005 Nov 3;48(3):403-15
references.pmid: 16269359
references.citation: Evol Dev. 2009 Jul-Aug;11(4):391-404
references.pmid: 19601973
references.citation: Q Rev Biol. 1951 Sep;26(3):264-80
references.pmid: 14875937
references.citation: Science. 1988 May 13;240(4854):901-5
references.pmid: 3363372
references.citation: Biol Lett. 2010 Jun 23;6(3):402-5
references.pmid: 20181553
references.citation: Exp Neurol. 1997 Nov;148(1):360-6
references.pmid: 9398478
references.citation: J Neurosci. 2008 Aug 20;28(34):8442-53
references.pmid: 18716202
references.citation: Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7558-62
references.pmid: 10377454
references.citation: Genesis. 2006 Mar;44(3):136-42
references.pmid: 16496337
references.citation: Trends Neurosci. 2010 Apr;33(4):183-92
references.pmid: 20153060
references.citation: Mech Dev. 2001 Jul;105(1-2):69-77
references.pmid: 11429283
references.citation: Dev Dyn. 2010 Jul;239(7):2058-65
references.pmid: 20549741
references.citation: PLoS One. 2009;4(2):e4477
references.pmid: 19223970
references.citation: J Neurosci. 1993 Oct;13(10):4101-13
references.pmid: 8410180
references.citation: Genes Dev. 2007 Sep 1;21(17):2118-30
references.pmid: 17785522
references.citation: Development. 2002 Feb;129(3):597-604
references.pmid: 11830561
references.citation: C R Acad Hebd Seances Acad Sci D. 1974 Jul 29;279(5):413-6
references.pmid: 4373185
references.citation: Brain Res. 1990 May 28;517(1-2):88-98
references.pmid: 2376010
references.citation: Development. 2010 May;137(10):1635-43
references.pmid: 20430744
references.citation: Proc Natl Acad Sci U S A. 1987 May;84(9):3064-8
references.pmid: 3495007
references.citation: Development. 2002 Feb;129(3):605-15
references.pmid: 11830562
references.citation: Curr Opin Neurobiol. 2004 Feb;14(1):67-73
references.pmid: 15018940
references.citation: J Physiol. 1970 Feb;206(2):419-36
references.pmid: 5498493
references.citation: Dev Dyn. 2003 Nov;228(3):370-85
references.pmid: 14579376
references.citation: J Comp Neurol. 1985 Mar 15;233(3):377-89
references.pmid: 3980776
references.citation: Neuron. 1996 Dec;17(6):1145-55
references.pmid: 8982162
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21948-53
references.pmid: 19996172
references.citation: Nature. 1979 Oct 25;281(5733):675-7
references.pmid: 45121
references.citation: J Neurosci. 2010 Jun 16;30(24):8234-44
references.pmid: 20554875
references.citation: Brain Behav Evol. 1996;48(2):55-69
references.pmid: 8853873