title: Geranylgeranylacetone protects against myocardial ischemia and reperfusion             injury by inhibiting high-mobility group box 1 protein in rats.
abstract: The high mobility group box 1 (HMGB1) protein plays an important role in myocardial ischemia and reperfusion (I/R) injury. Geranylgeranylacetone (GGA), a heat shock protein 72 inducer, has been reported to reduce myocardial I/R injury. The aim of this study was to investigate the cardioprotective mechanism of GGA during myocardial I/R injury in rats. Anesthetized male rats were treated once with GGA (200 mg/kg, p.o.) 24 h before ischemia, and subjected to ischemia for 30 min, followed by reperfusion for 4 h. Lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA), superoxide dismutase (SOD) activity and infarct size were measured. HMGB1 expression was assessed by immunoblotting. The results showed that pre-treatment with GGA (200 mg/kg) significantly reduced the infarct size and the levels of LDH and CK after 4 h of reperfusion (all P<0.05). GGA also significantly inhibited the increase in MDA levels and the decrease in SOD levels (both P<0.05). Meanwhile, GGA considerably suppressed the expression of HMGB1 induced by I/R. The present study suggests that GGA is capable of attenuating myocardial I/R injury by inhibiting HMGB1 expression.
authors.affiliation: Department of Cardiology, Dongfeng Hospital of Hubei Medical University, Shiyan, Hubei 442008, PR China. wangneng0701@163.com
authors.forename: Neng
authors.initials: N
authors.lastname: Wang
authors.forename: Xinwen
authors.initials: X
authors.lastname: Min
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Li
authors.forename: Peigen
authors.initials: P
authors.lastname: He
authors.forename: Libin
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003402:Creatine Kinase / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects*; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors* / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D017202:Myocardial Ischemia / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D004224:Diterpenes; D024243:HMGB1 Protein; D050884:HSP72 Heat-Shock Proteins; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; C031049:geranylgeranylacetone