title: Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption.
abstract: The Chaihu-Shaoyao drug pair (Bupleuri Radix and Paeoniae Radix Alba) which is a traditional Chinese drug pair, has been widely used for anti-inflammatory purposes. Saikosaponin a (SSA), saikosaponin d (SSD) and paeoniflorin are identified as the main components in the pair. The present study focused on the interaction of the main components based on investigating their intestinal absorption using a four-site perfused rat intestinal model in order to clarify the mechanism of the compatibility of Chaihu-Shaoyao. The concentrations of SSA, SSD and paeoniflorin in the intestinal perfusate were determined by LC/MS or UPLC (Ultra Performance Liquid Chromatography) methods, followed by P*(eff) (effective permeability) and 10% ABS (the percent absorption of 10 cm of intestine) calculations. The results showed that all of the three main components displayed very low permeabilities (P*(eff) < 0.4), which implied their poor absorption in the rat intestine. The absorption levels of SSA and SSD were similar in intestine and higher in ileum than those in other intestinal regions in the decreasing order: colon, jejunum and duodenum. However, there is no significant difference in the absorption of paeoniflorin in the four segments (P < 0.05). The P*(eff) values of paeoniflorin exhibited an almost 2.11-fold or 1.90-fold increase in ileum when it was co-administrated with SSA and SSD, as well as 2.42-, 2.18-fold increase in colon, respectively, whereas the absorptions of SSA and SSD were not influenced by paeoniflorin. In conclusion, SSA and SSD could promote the absorption of paeoniflorin. To some extent this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Material Medica, Jiangsu Province, Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, China. ychen202@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Yuan
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Tan
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology; D001565:Benzoates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D029972:Bupleurum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000033:anatomy & histology; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D039821:Monoterpenes; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D009828:Oleanolic Acid; D012503:Saponins; C015423:peoniflorin; D006027:Glycosides; C025759:saikosaponin D
references.citation: Neurosci Lett. 2009 Jan 16;449(3):215-9
references.pmid: 19007859
references.citation: J Pharmacol Exp Ther. 2003 Mar;304(3):1228-35
references.pmid: 12604700
references.citation: Life Sci. 2009 Mar 13;84(11-12):337-44
references.pmid: 19211027
references.citation: Pharm Res. 2008 Sep;25(9):2190-9
references.pmid: 18459036
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 5;812(1-2):71-84
references.pmid: 15556489
references.citation: J Pharm Sci. 1998 Jul;87(7):886-90
references.pmid: 9649359
references.citation: J Pharm Pharmacol. 2003 Mar;55(3):313-21
references.pmid: 12724036
references.citation: Biomed Chromatogr. 2008 Dec;22(12):1385-92
references.pmid: 18655214
references.citation: Int Immunopharmacol. 2009 Nov;9(12):1437-43
references.pmid: 19733696
references.citation: Molecules. 2009 Oct 09;14(10):3942-51
references.pmid: 19924040
references.citation: Molecules. 2011 Feb 01;16(2):1336-48
references.pmid: 21285919
references.citation: Arzneimittelforschung. 2002;52(9):684-8
references.pmid: 12404883
references.citation: Life Sci. 2004 Jul 23;75(10):1231-42
references.pmid: 15219811
references.citation: Life Sci. 2004 Dec 31;76(7):813-26
references.pmid: 15581913
references.citation: J Ethnopharmacol. 2005 May 13;99(1):61-7
references.pmid: 15848021
references.citation: Eur J Pharm Sci. 2009 Feb 15;36(2-3):320-9
references.pmid: 19028572
references.citation: Arch Pharm Res. 2009 Oct;32(10):1417-23
references.pmid: 19898805
references.citation: J Ethnopharmacol. 2010 Jul 20;130(2):407-13
references.pmid: 20580804
references.citation: Front Pharmacol. 2011 Feb 25;2:10
references.pmid: 21687505
references.citation: J Zhejiang Univ Sci B. 2006 Feb;7(2):99-106
references.pmid: 16421964
references.citation: Neurogastroenterol Motil. 2010 Jan;22(1):e15-26
references.pmid: 19614866