title: A seven-transmembrane receptor that mediates avoidance response to dihydrocaffeic acid, a water-soluble repellent in Caenorhabditis elegans.
abstract: The ability to detect harmful chemicals rapidly is essential for the survival of all animals. In Caenorhabditis elegans (C. elegans), repellents trigger an avoidance response, causing animals to move away from repellents. Dihydrocaffeic acid (DHCA) is a water-soluble repellent and nonflavonoid catecholic compound that can be found in plant products. Using a Xenopus laevis (X. laevis) oocyte expression system, we identified a candidate dihydrocaffeic acid receptor (DCAR), DCAR-1. DCAR-1 is a novel seven-transmembrane protein that is expressed in the ASH avoidance sensory neurons of C. elegans. dcar-1 mutant animals are defective in avoidance response to DHCA, and cell-specific expression of dcar-1 in the ASH neurons of dcar-1 mutant animals rescued the defect in avoidance response to DHCA. Our findings identify DCAR-1 as the first seven-transmembrane receptor required for avoidance of a water-soluble repellent, DHCA, in C. elegans.
authors.affiliation: Department of Molecular Pharmacology and Neurobiology, Yokohama City University, Graduate School of Medicine, Yokohama 236-0004, Japan.
authors.forename: Reina
authors.initials: R
authors.lastname: Aoki
authors.forename: Tatsurou
authors.initials: T
authors.lastname: Yagami
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sasakura
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ogura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ibi
authors.forename: Takeaki
authors.initials: T
authors.lastname: Miyamae
authors.forename: Fumio
authors.initials: F
authors.lastname: Nakamura
authors.forename: Taro
authors.initials: T
authors.lastname: Asakura
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Kanai
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Misu
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Iino
authors.forename: Marina
authors.initials: M
authors.lastname: Ezcurra
authors.forename: William R
authors.initials: WR
authors.lastname: Schafer
authors.forename: Ikue
authors.initials: I
authors.lastname: Mori
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Goshima
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002109:Caffeic Acids / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004924:Escape Reaction / Q000187:drug effects* / Q000502:physiology; D062385:Hydroxybenzoates; D007814:Larva; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D008845:Microinjections / Q000379:methods; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000502:physiology; D014981:Xenopus
chemical_list: D029742:Caenorhabditis elegans Proteins; D002109:Caffeic Acids; D002396:Catechols; D062385:Hydroxybenzoates; D008164:Luminescent Proteins; D043562:Receptors, G-Protein-Coupled; C568999:STR-33 protein, C elegans; C569206:dihydrocaffeic acid receptor 1, C elegans; D015102:3,4-Dihydroxyphenylacetic Acid; C000995:3,4-dihydroxyphenylpropionic acid; C009135:2,3-dihydroxybenzoic acid
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