title: Use of autologous chondrocytes and bioinert perforated chambers to tissue engineer cartilage in vivo.
abstract: OBJECTIVE
  To explore the potential applications of a chamber for in vivo tissue engineering, and to establish a novel model for in vivo tissue-engineered cartilage.
  
  
  METHODS
  Four experimental groups were included in this study: (A) chambers + chondrocytes/collagen gel; (B) chambers + chondrocytes/PLGA gel; (C) chondrocytes/collagen gel alone; and (D) chondrocytes/PLGA gel alone. Groups C and D served as controls. The samples were implanted subcutaneously in the donor rabbit, and the contents were harvested at 8 wk after implantation.
  
  
  RESULTS
  Histologic and immunohistochemical staining and RT-PCR results revealed regenerated cartilage-like tissue in group B and small, irregularly shaped islands of opalescent tissue in group A. In contrast, the control groups displayed vascular invasion and inflammatory reaction, which eventually led to fibrosis and absorption.
  
  
  CONCLUSIONS
  Reproduced cartilages were obtained in an immunocompetent animal model through the use of a bioinert perforated chamber.
authors.affiliation: Institute of Plastic Surgery of People's Liberation Army, Xijing Hospital, Fourth Military Medical University, Xi'an Shaanxi, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Jiang
authors.forename: Jianxue
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Hao
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Diao
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Guo
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005782:Gels / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D011100:Polyglycolic Acid / Q000378:metabolism; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D014182:Transplantation, Autologous
chemical_list: D005782:Gels; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen