title: LC-MS-based metabolomics in the clinical laboratory.
abstract: The analysis of metabolites in human body fluids remains a challenge because of their chemical diversity and dynamic concentration range. Liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) offers a robust, reliable, and economical methodology for quantitative single metabolite analysis and profiling of complete metabolite classes of a biological specimen over a broad dynamic concentration range. The application of LC-MS/MS based metabolomic approaches in clinical applications aims at both, the improvement of diagnostic sensitivity and specificity by profiling a metabolite class instead of a single metabolite analysis, and the identification of new disease specific biomarkers. In the present paper we discuss recent advances in method development for LC-MS/MS analysis of lipids, carbohydrates, amino acids and biogenic amines, vitamins and organic acids with focus on human body fluids. In this context an overview on recent LC-MS/MS based metabolome studies for cancer, diabetes and coronary heart disease is presented.
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Leipzig, Germany.
authors.forename: Susen
authors.initials: S
authors.lastname: Becker
authors.forename: Linda
authors.initials: L
authors.lastname: Kortz
authors.forename: Christin
authors.initials: C
authors.lastname: Helmschrodt
authors.forename: Joachim
authors.initials: J
authors.lastname: Thiery
authors.forename: Uta
authors.initials: U
authors.lastname: Ceglarek
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D002624:Chemistry, Clinical / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000032:analysis; D055432:Metabolomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014815:Vitamins / Q000032:analysis
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines; D002241:Carbohydrates; D008055:Lipids; D014815:Vitamins