title: Application of low magnetic field on inulinase production by Geotrichum candidum under solid state fermentation using leek as substrate.
abstract: This study evaluates the application of low magnetic field (LMF) on inulinase enzyme production by Geotrichum candidum under solid state fermentation (SSF) using leek as potential carbon source. First, the fermentation conditions were optimized using normal magnetic field grown microorganism. Among eight G. candidum isolates, the most effective strain called G. candidum OC-7 was selected to use in further experiments. In the second part of the study, SSF was carried out under different LMFs (4 and 7 mT). The results showed that inulinase activity was strongly affected by LMF application. The highest enzyme activity was obtained as 535.2 U/g of dry substrate (gds) by 7 mT magnetic field grown G. candidum OC-7. On the contrary, the control had only 412.1 U/gds. Consequently, the use of leek presents a great potential as an alternative carbon source for inulinase production and magnetic field treatment could effectively be used in order to enhance the enzyme production.
authors.affiliation: Department of Biology, Ataturk University, Erzurum, Turkey. ozdencanli83@hotmail.com
authors.forename: Ozden
authors.initials: O
authors.lastname: Canli
authors.forename: Esabi Basaran
authors.initials: EB
authors.lastname: Kurbanoglu
mesh_terms: D000704:Analysis of Variance; D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation / Q000528:radiation effects; D005848:Geotrichum / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D060526:Magnetic Fields; D019697:Onions / Q000382:microbiology*
chemical_list: D006026:Glycoside Hydrolases; C023086:inulinase