title: Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column.
abstract: In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25°C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth.
authors.affiliation: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. clarissenobre@deb.uminho.pt
authors.forename: C
authors.initials: C
authors.lastname: Nobre
authors.forename: J A
authors.initials: JA
authors.lastname: Teixeira
authors.forename: L R
authors.initials: LR
authors.lastname: Rodrigues
mesh_terms: D000327:Adsorption; D002606:Charcoal / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry*; D005632:Fructose / Q000096:biosynthesis / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification*; D004718:Saccharomycetales / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; D002606:Charcoal; D005632:Fructose