title: Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy.
abstract: A synchronous fluorescence spectroscopy (SFS) method was developed for determination of pyrene in the gills of exposed fish. The wavelength differences (Δλ) of 50 nm was maintained between excitation and emission wavelengths and it was found to be suitable for the effective determination of pyrene in fish gills; the peak were observed at λ(ex) 334.5 nm. Linear relationships between SFS intensity and the concentration of pyrene in n-hexane solution were established. It was demonstrated that the SFS method was effective, simple, and less expensive, providing an attractive alternative for the rapid analysis of pyrene in fish gills.
authors.affiliation: Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Huangshi Institute of Technology, Huangshi 435003, PR China.
authors.forename: Xianli
authors.initials: X
authors.lastname: Liu
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Jing
authors.forename: Shengfang
authors.initials: S
authors.lastname: Li
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Zou
authors.forename: Xianyou
authors.initials: X
authors.lastname: Xia
authors.forename: Weidong
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes; D005880:Gills / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry; D011721:Pyrenes / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D006586:Hexanes; D011721:Pyrenes; C026385:n-hexane; C030984:pyrene