title: [Extraction, purification technology and antineoplastic effects of solamargine].
abstract: OBJECTIVE
  To extract and purify of solamargine from Solanum nigrum, and to research its antineoplastic effects.
  
  
  METHODS
  S. nigrum was extracted refluently with 80% alcohol, solamargine was purified with silica gel column chromatography and recrystallization, and then conducted its structure identification and purity checks. Screened the effect on human tumor cell groth inhibition in vitro by MTT assay, and researched on the features in mice with H22 liver cancer or Ehrlich ascites tumor of solamargine.
  
  
  RESULTS
  The concent of solamargine reached 97.9%. Solamargine had significantly inhibition on 6 tumor cells in vitro, and it had significantly inhibition on mice with H22 liver cancer or ehrlich ascites tumor in the 2.4 mg x kg(-1) dose of i.v.
  
  
  CONCLUSIONS
  Solamargine have the antineoplastic effect.
authors.affiliation: Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Lianming
authors.initials: L
authors.lastname: Xu
authors.forename: Binjiang
authors.initials: B
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yu'an
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D012991:Solanaceous Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012991:Solanaceous Alkaloids; C026609:beta-solamarine