title: Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics.
abstract: Myogenesis, the formation of skeletal muscle, is a multistep event that commences with myoblast proliferation, followed by cell-cycle arrest, and finally the formation of multinucleated myotubes via fusion of mononucleated myoblasts. Each step is orchestrated by well-documented intracellular factors, such as cytoplasmic signalling molecules and nuclear transcription factors. Regardless, the key step in getting a more comprehensive understanding of the regulation of myogenesis is to explore the extracellular factors that are capable of eliciting the downstream intracellular factors. This could further provide valuable insight into the acute cellular response to extrinsic cues in maintaining normal muscle development. In this paper, we survey the intracellular factors that respond to extracellular cues that are responsible for the cascades of events during myogenesis: myoblast proliferation, cell-cycle arrest of myoblasts, and differentiation of myoblasts into myotubes. This focus on extracellular perspective of muscle development illustrates our mass spectrometry-based proteomic approaches to identify differentially expressed secreted factors during skeletal myogenesis.
authors.affiliation: Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3.
authors.forename: C Y X'avia
authors.initials: CY
authors.lastname: Chan
authors.forename: John C
authors.initials: JC
authors.lastname: McDermott
authors.forename: K W Michael
authors.initials: KW
authors.lastname: Siu
references.citation: FEBS Lett. 2008 Jun 11;582(13):1913-8
references.pmid: 18501712
references.citation: Mol Cell Biol. 1989 Nov;9(11):5022-33
references.pmid: 2601707
references.citation: J Biol Chem. 2000 Nov 3;275(44):34359-64
references.pmid: 10944526
references.citation: Mol Biol Cell. 2005 Mar;16(3):1469-80
references.pmid: 15647380
references.citation: Cancer Biol Ther. 2007 Oct;6(10):1539-45
references.pmid: 18293492
references.citation: Mol Cell Biol. 1997 Feb;17(2):1010-26
references.pmid: 9001254
references.citation: Front Biosci. 2000 Jan 01;5:D121-37
references.pmid: 10702384
references.citation: J Proteome Res. 2005 Sep-Oct;4(5):1661-71
references.pmid: 16212419
references.citation: Amino Acids. 2011 Jul;41(2):387-99
references.pmid: 20617350
references.citation: Lasers Med Sci. 2003;18(2):78-82
references.pmid: 12928816
references.citation: Int J Cancer. 2004 Sep 10;111(4):514-21
references.pmid: 15239128
references.citation: J Cell Biol. 2006 Nov 6;175(3):383-8
references.pmid: 17074887
references.citation: J Exp Med. 2004 Feb 16;199(4):581-92
references.pmid: 14970183
references.citation: Cell. 2008 Jul 25;134(2):353-64
references.pmid: 18662549
references.citation: Mol Cell Biol. 2009 Aug;29(15):4130-43
references.pmid: 19470755
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):552-7
references.pmid: 9892671
references.citation: Mol Cell Proteomics. 2010 Oct;9(10):2173-83
references.pmid: 20525996
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1206-10
references.pmid: 14745025
references.citation: Proteomics. 2010 Mar;10(5):1029-39
references.pmid: 20049865
references.citation: J Biol Chem. 1996 Apr 12;271(15):9009-13
references.pmid: 8621548
references.citation: DNA Cell Biol. 1990 Jun;9(5):303-9
references.pmid: 2372374
references.citation: J Biol Chem. 2004 Oct 1;279(40):41626-33
references.pmid: 15292192
references.citation: Dev Dyn. 2005 Jun;233(2):266-81
references.pmid: 15858817
references.citation: Nat Methods. 2010 May;7(5):383-5
references.pmid: 20364148
references.citation: Proteomics. 2005 Jul;5(11):2960-71
references.pmid: 15986332
references.citation: J Cell Biol. 2002 Apr 1;157(1):137-48
references.pmid: 11927606
references.citation: J Cell Biol. 1997 Jul 14;138(1):203-13
references.pmid: 9214393
references.citation: Dev Biol. 2009 Aug 1;332(1):116-30
references.pmid: 19464283
references.citation: Science. 1996 Jun 7;272(5267):1476-80
references.pmid: 8633239
references.citation: Mol Cell Biol. 2004 Feb;24(4):1809-21
references.pmid: 14749395
references.citation: Mol Vis. 2003 Jun 30;9:277-87
references.pmid: 12835654
references.citation: Nature. 1988 Dec 22-29;336(6201):772-4
references.pmid: 3205305
references.citation: J Cell Biol. 1998 Oct 19;143(2):403-13
references.pmid: 9786951
references.citation: J Biol Chem. 2004 Aug 27;279(35):36795-802
references.pmid: 15194693
references.citation: Cell. 1999 Sep 17;98(6):859-69
references.pmid: 10499802
references.citation: Genes Dev. 2001 Nov 15;15(22):2950-66
references.pmid: 11711431
references.citation: Genes Dev. 1993 Dec;7(12B):2609-17
references.pmid: 8276243
references.citation: Cell Biol Toxicol. 2003 Oct;19(5):285-98
references.pmid: 14703116
references.citation: Mol Cell Biol. 1998 Sep;18(9):4994-9
references.pmid: 9710583
references.citation: Mol Cell Proteomics. 2004 Jul;3(7):729-35
references.pmid: 15102926
references.citation: J Embryol Exp Morphol. 1981 Dec;66:1-26
references.pmid: 7338706
references.citation: Am J Physiol Cell Physiol. 2010 Apr;298(4):C807-16
references.pmid: 20089933
references.citation: J Biol Chem. 1992 Jun 5;267(16):10956-60
references.pmid: 1317849
references.citation: Arch Biochem Biophys. 2007 Sep 1;465(1):11-5
references.pmid: 17543881
references.citation: Clin Cancer Res. 2008 Apr 15;14(8):2326-33
references.pmid: 18413821
references.citation: J Biol Chem. 2000 Jun 23;275(25):18767-76
references.pmid: 10764802
references.citation: Arterioscler Thromb Vasc Biol. 1997 Nov;17(11):2437-47
references.pmid: 9409213
references.citation: Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10
references.pmid: 3022285
references.citation: Cancer Res. 2004 Dec 15;64(24):9018-26
references.pmid: 15604267
references.citation: J Proteome Res. 2007 Feb;6(2):698-710
references.pmid: 17269726
references.citation: Eur J Cell Biol. 1991 Aug;55(2):209-16
references.pmid: 1935986
references.citation: J Cell Biol. 2008 Aug 11;182(3):497-507
references.pmid: 18678706
references.citation: Biochem Biophys Res Commun. 2000 May 19;271(3):630-4
references.pmid: 10814513
references.citation: Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4212-7
references.pmid: 20160094
references.citation: Dev Biol. 1987 Mar;120(1):215-27
references.pmid: 3817290
references.citation: J Biol Chem. 2001 Jun 22;276(25):22468-75
references.pmid: 11309375
references.citation: EMBO J. 1998 Mar 2;17(5):1412-22
references.pmid: 9482738
references.citation: Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):6914-8
references.pmid: 8346196
references.citation: Cell. 1987 Dec 24;51(6):987-1000
references.pmid: 3690668
references.citation: J Biol Chem. 2001 Apr 13;276(15):12201-11
references.pmid: 11152692
references.citation: Development. 1998 Sep;125(17):3437-43
references.pmid: 9693147
references.citation: EMBO J. 2001 Dec 3;20(23):6816-25
references.pmid: 11726517
references.citation: Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9366-73
references.pmid: 8790335
references.citation: Clin Cancer Res. 2007 Jul 1;13(13):3875-82
references.pmid: 17606720
references.citation: J Histochem Cytochem. 2009 Jan;57(1):29-39
references.pmid: 18796407
references.citation: Annu Rev Biochem. 1998;67:609-52
references.pmid: 9759499
references.citation: Matrix Biol. 1998 Apr;17(1):1-19
references.pmid: 9628249
references.citation: Eur J Biochem. 1993 Nov 15;218(1):117-27
references.pmid: 7916692
references.citation: Nat Genet. 1998 Sep;20(1):78-82
references.pmid: 9731537
references.citation: Adv Exp Med Biol. 1993;343:319-26
references.pmid: 8184742
references.citation: Connect Tissue Res. 1993;30(1):11-21
references.pmid: 7512011
references.citation: Cancer Biol Ther. 2010 Oct 15;10(8):811-3
references.pmid: 20953140
references.citation: J Biol Chem. 1995 May 19;270(20):12109-16
references.pmid: 7744859
references.citation: J Cell Sci. 2004 May 1;117(Pt 11):2173-81
references.pmid: 15126619
references.citation: Cell. 1991 Jul 26;66(2):305-15
references.pmid: 1649701
references.citation: Dev Cell. 2004 Dec;7(6):843-54
references.pmid: 15572127
references.citation: EMBO J. 1989 Mar;8(3):701-9
references.pmid: 2721498
references.citation: J Biol Chem. 1993 May 25;268(15):11050-6
references.pmid: 8496166
references.citation: Curr Biol. 1993 Jan;3(1):1-11
references.pmid: 15335872
references.citation: Acta Biomed. 2007;78 Suppl 1:156-206
references.pmid: 17465332
references.citation: Mol Cell Biol. 2000 Jun;20(11):3951-64
references.pmid: 10805738
references.citation: Glia. 2008 Apr 15;56(6):675-85
references.pmid: 18293395
references.citation: Physiol Rev. 2008 Oct;88(4):1379-406
references.pmid: 18923185
references.citation: J Biol Chem. 1999 Jul 2;274(27):18843-6
references.pmid: 10383378
references.citation: Proteins. 2000 Feb 1;38(2):210-25
references.pmid: 10656267
references.citation: Biochem J. 1985 Feb 1;225(3):619-27
references.pmid: 2983679
references.citation: J Biol Chem. 1986 Dec 15;261(35):16509-13
references.pmid: 3465726
references.citation: Proteomics. 2010 Jun;10(11):2165-74
references.pmid: 20340163
references.citation: Circ Res. 2006 May 12;98(9):1194-202
references.pmid: 16574905
references.citation: Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5242-6
references.pmid: 8506372
references.citation: Genes Dev. 2002 Jan 15;16(2):245-56
references.pmid: 11799067
references.citation: Mol Biol Cell. 1998 Nov;9(11):3119-31
references.pmid: 9802901
references.citation: J Biol Chem. 2001 Apr 13;276(15):12212-21
references.pmid: 11152695
references.citation: Cell. 1993 Oct 8;75(1):59-72
references.pmid: 8402901
references.citation: Matrix Biol. 2007 Jul;26(6):431-41
references.pmid: 17509843
references.citation: Development. 1990 Aug;109(4):943-52
references.pmid: 2226207
references.citation: Dev Biol. 1986 May;115(1):140-7
references.pmid: 3699242
references.citation: Cancer Biol Ther. 2010 Oct 15;10(8):796-810
references.pmid: 20686364
references.citation: Mol Cell Proteomics. 2009 May;8(5):959-70
references.pmid: 19151416
references.citation: Mol Cell Biol. 2001 Aug;21(16):5312-20
references.pmid: 11463815
references.citation: Mol Cell Proteomics. 2005 Sep;4(9):1297-310
references.pmid: 15961381
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15603-7
references.pmid: 9861016
references.citation: Mol Cell Biol. 1997 Nov;17(11):6563-73
references.pmid: 9343420
references.citation: FEBS Lett. 2004 Jul 2;569(1-3):1-6
references.pmid: 15225599
references.citation: Proc Natl Acad Sci U S A. 1990 Oct;87(20):7988-92
references.pmid: 2172969
references.citation: J Biol Chem. 1996 May 31;271(22):12699-702
references.pmid: 8663030
references.citation: Cell. 1995 Dec 29;83(7):1125-36
references.pmid: 8548800
references.citation: Cancer Biol Ther. 2009 Jun;8(11):1064-70
references.pmid: 19471113
references.citation: Mol Cell Proteomics. 2004 Dec;3(12):1154-69
references.pmid: 15385600
references.citation: J Biol Chem. 1997 Oct 31;272(44):28089-95
references.pmid: 9346963
references.citation: Cancer Lett. 2009 Apr 8;276(1):88-94
references.pmid: 19081179
references.citation: J Cell Biol. 2000 Dec 11;151(6):1131-40
references.pmid: 11121430
references.citation: J Biol Chem. 2004 May 7;279(19):19391-5
references.pmid: 15044443
references.citation: J Cell Sci. 1995 Sep;108 ( Pt 9):2973-81
references.pmid: 8537437
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7017-21
references.pmid: 8041738
references.citation: BMC Cancer. 2010 Sep 03;10:477
references.pmid: 20815902
references.citation: Exp Cell Res. 1990 Jun;188(2):175-84
references.pmid: 2335185
references.citation: Nat Biotechnol. 1999 Oct;17(10):994-9
references.pmid: 10504701
references.citation: Cell. 1989 Sep 22;58(6):1097-105
references.pmid: 2673543
references.citation: Genes Dev. 1992 Sep;6(9):1783-98
references.pmid: 1516833
references.citation: Mol Cell Biol. 2000 Dec;20(23):8983-95
references.pmid: 11073997
references.citation: Differentiation. 2004 Sep;72(7):341-7
references.pmid: 15554945
references.citation: Biochem Biophys Res Commun. 2001 Nov 30;289(2):507-12
references.pmid: 11716502
references.citation: Curr Opin Genet Dev. 1996 Apr;6(2):176-84
references.pmid: 8722174
references.citation: J Am Soc Mass Spectrom. 2010 Jun;21(6):879-89
references.pmid: 20199872
references.citation: Dev Biol. 1997 May 1;185(1):14-24
references.pmid: 9169046
references.citation: J Biol Chem. 1998 Jan 2;273(1):157-66
references.pmid: 9417060
references.citation: Mol Cell Proteomics. 2002 May;1(5):376-86
references.pmid: 12118079
references.citation: Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11599-604
references.pmid: 12193653
references.citation: Int J Radiat Oncol Biol Phys. 2006 Dec 1;66(5):1461-72
references.pmid: 17084559
references.citation: Nature. 1996 Nov 21;384(6606):266-70
references.pmid: 8918877
references.citation: Cell. 1996 Sep 6;86(5):731-41
references.pmid: 8797820
references.citation: J Proteome Res. 2008 Nov;7(11):4715-26
references.pmid: 18816084
references.citation: Oncogene. 2006 Aug 28;25(38):5244-9
references.pmid: 16936743
references.citation: EMBO J. 2007 Mar 7;26(5):1245-56
references.pmid: 17304211
references.citation: EMBO J. 2005 Mar 9;24(5):974-84
references.pmid: 15719023
references.citation: Oncogene. 1998 Dec 24;17(25):3365-83
references.pmid: 9916999
references.citation: Neuroreport. 1997 Nov 10;8(16):3649-54
references.pmid: 9427343
references.citation: Methods Mol Biol. 2010;662:59-78
references.pmid: 20824466
references.citation: Cell. 1993 Sep 24;74(6):1033-42
references.pmid: 7691411
references.citation: Mol Vis. 2002 Oct 31;8:407-15
references.pmid: 12432342
references.citation: Nat Med. 2007 Feb;13(2):204-10
references.pmid: 17237794
references.citation: Nat Chem Biol. 2005 Oct;1(5):252-62
references.pmid: 16408053
references.citation: Acta Biochim Biophys Sin (Shanghai). 2008 Apr;40(4):349-55
references.pmid: 18401533
references.citation: J Biol Chem. 1999 Feb 19;274(8):5193-200
references.pmid: 9988769
references.citation: Dig Dis Sci. 2010 Dec;55(12):3408-14
references.pmid: 20411424
references.citation: J Biol Chem. 1989 Dec 5;264(34):20805-10
references.pmid: 2584240
references.citation: Int J Radiat Oncol Biol Phys. 2006 Feb 1;64(2):581-91
references.pmid: 16414373
references.citation: Expert Opin Ther Targets. 2010 Jun;14(6):603-10
references.pmid: 20367238
references.citation: J Biol Chem. 1999 Feb 12;274(7):4341-6
references.pmid: 9933636
references.citation: Anat Embryol (Berl). 1995 May;191(5):381-96
references.pmid: 7625610
references.citation: Mol Cell Biol. 2000 May;20(9):3256-65
references.pmid: 10757809
references.citation: Oncogene. 1995 Sep 21;11(6):1199-205
references.pmid: 7566981
references.citation: Cell. 1989 Sep 22;58(6):1085-95
references.pmid: 2673542
references.citation: Cell. 1989 Sep 22;58(6):1193-8
references.pmid: 2673546
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11593-8
references.pmid: 15289617
references.citation: Cancer Res. 2006 Jul 15;66(14):7136-42
references.pmid: 16849559
references.citation: Mol Cell. 1999 Nov;4(5):725-34
references.pmid: 10619020
references.citation: Mol Cell. 1997 Dec;1(1):35-45
references.pmid: 9659901
references.citation: Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10844-8
references.pmid: 7971972
references.citation: J Biol Chem. 2002 Dec 20;277(51):49927-34
references.pmid: 12372839
references.citation: Cell. 1990 Apr 6;61(1):49-59
references.pmid: 2156629
references.citation: Proteomics. 2008 Dec;8(23-24):5108-18
references.pmid: 19016532
references.citation: FEBS Lett. 2005 May 23;579(13):2873-7
references.pmid: 15876430
references.citation: EMBO J. 1997 Jan 15;16(2):369-83
references.pmid: 9029156
references.citation: Trends Cell Biol. 2006 Jan;16(1):36-44
references.pmid: 16325404
references.citation: Dev Biol. 1994 Oct;165(2):537-44
references.pmid: 7958419
references.citation: Biotechnol Appl Biochem. 2009 Jul 06;54(1):11-20
references.pmid: 19250064
references.citation: EMBO J. 2004 Apr 7;23(7):1557-66
references.pmid: 15044954
references.citation: Genes Dev. 1993 Mar;7(3):331-42
references.pmid: 8449399
references.citation: J Anat. 2003 Jan;202(1):59-68
references.pmid: 12587921
references.citation: Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2254-8
references.pmid: 7892257
references.citation: World J Gastroenterol. 2009 Dec 28;15(48):6117-22
references.pmid: 20027687
references.citation: Development. 1992 Feb;114(2):339-53
references.pmid: 1591996
references.citation: Mol Cell Proteomics. 2008 Apr;7(4):672-83
references.pmid: 18045802
references.citation: J Biol Chem. 2008 Nov 21;283(47):32802-11
references.pmid: 18718903
references.citation: J Cell Biol. 1986 Nov;103(5):1799-805
references.pmid: 3465734
references.citation: J Orthop Res. 2010 Aug;28(8):1040-9
references.pmid: 20108312
references.citation: J Biol Chem. 1993 Sep 5;268(25):18633-6
references.pmid: 8360158
references.citation: Cancer Lett. 2000 Aug 1;156(1):27-35
references.pmid: 10840156
references.citation: Proc Natl Acad Sci U S A. 1991 May 1;88(9):3822-6
references.pmid: 1850837
references.citation: Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12358-63
references.pmid: 9770491
references.citation: Mol Cell Biochem. 2003 Apr;246(1-2):3-11
references.pmid: 12841336
references.citation: Mol Cell Proteomics. 2011 Feb;10(2):M110.000679
references.pmid: 20530636
references.citation: J Cell Physiol. 1982 Feb;110(2):190-5
references.pmid: 7040426
references.citation: Nat Genet. 2004 Jul;36(7):738-43
references.pmid: 15208625
references.citation: Hybridoma (Larchmt). 2005 Jun;24(3):141-5
references.pmid: 15943561
references.citation: Mol Cell Biol. 1999 Apr;19(4):3167-76
references.pmid: 10082583