title: Effects of biodegradable Mg-6Zn alloy extracts on cell cycle of intestinal epithelial cells.
abstract: In this study, intestinal epithelial cells (IEC)-6 were cultured in different concentration extracts of Mg-6Zn alloys for different time periods. We studied the indirect effects of Mg-6Zn alloys on cell cycle of IEC-6 cells. The cell cycle of IEC-6 cells was measured using flow cytometry. And, the cell cycle of IEC-6 cells was evaluated by investigating the expression of cyclin D1, CDK4, and P21 using real-time polymerase chain reaction (PCR) and Western blotting tests. It was found that the IEC-6 cells displayed better cell functions in 20% extract of the Mg-6Zn alloy extracts, compared to the 100% or 60% extract. The in vitro results indicated that the conspicuous alkaline environment that is a result of rapid corrosion of Mg-6Zn alloys is disadvantageous to cell cycle of IEC-6 cells.
authors.affiliation: Shanghai Jiaotong University School of Medicine, PR China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianan
authors.initials: J
authors.lastname: Li
authors.forename: Xiaonong
authors.initials: X
authors.lastname: Zhang
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D020341:Absorbable Implants / Q000009:adverse effects*; D000497:Alloys / Q000633:toxicity; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D003343:Corrosion; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008274:Magnesium / Q000633:toxicity*; D008422:Materials Testing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015032:Zinc / Q000633:toxicity*
chemical_list: D000497:Alloys; C530106:Ccnd1 protein, rat; C493958:Cdkn1a protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; C000592758:Mg-6Zn alloy; D012333:RNA, Messenger; D019938:Cyclin D1; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D008274:Magnesium; D015032:Zinc