title: Chemical modifications of algal mannans and xylomannans: effects on antiviral activity.
abstract: The structures of two sulfated xylomannans extracted from the red alga Nemalion helminthoides were determined. These two fractions plus a sulfated mannan, isolated from the same alga and whose structure was previously reported, were subjected to chemical modification. The mannan was oversulfated with SO(3)-pyridine in dimethyl sulfoxide at 60 °C during two and three hours and the xylomannans were subjected to Smith degradation in order to eliminate xylose side-chains. Structural analysis of all derivatives was carried out by methylation analysis and (13)C NMR spectroscopy. Antiviral activity against herpes simplex virus type 1 and 2, and dengue virus type 2 of native and modified mannans and xylomannans was estimated. Anticoagulant effect of the active fractions was also determined.
authors.affiliation: Departamento de Química Orgánica-CIHIDECAR, CONICET-UBA, Facultad de Ciencias Exactas y Naturales, UBA, Pabellón 2, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pérez Recalde
authors.forename: María J
authors.initials: MJ
authors.lastname: Carlucci
authors.forename: Miguel D
authors.initials: MD
authors.lastname: Noseda
authors.forename: María C
authors.initials: MC
authors.lastname: Matulewicz
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D003716:Dengue Virus / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects; D018258:Herpesvirus 2, Human / Q000187:drug effects; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D000461:Rhodophyta / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014994:Xylose / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000925:Anticoagulants; D000998:Antiviral Agents; D008351:Mannans; D013463:Sulfuric Acid Esters; D014994:Xylose