title: Differential effects on p53-mediated cell cycle arrest vs. apoptosis by p90.
abstract: p53 functions as a central node for organizing whether the cell responds to stress with apoptosis or cell cycle arrest; however, the molecular events that lead to apoptotic responses are not completely understood. Here, we identified p90 (also called Coiled-Coil Domain Containing 8) as a unique regulator for p53. p90 has no obvious effects on either the levels of p53 or p53-mediated cell cycle arrest but is specifically required for p53-mediated apoptosis upon DNA damage. Notably, p90 is crucial for Tip60-dependent p53 acetylation at Lys120, therefore facilitating activation of the proapoptotic targets. These studies indicate that p90 is a critical cofactor for p53-mediated apoptosis through promoting Tip60-mediated p53 acetylation.
authors.affiliation: Institute for Cancer Genetics, Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, 1130 St. Nicholas Avenue, New York, NY 10032, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Dai
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.forename: Sung Yun
authors.initials: SY
authors.lastname: Jung
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Aaronson
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics*; D002460:Cell Line; D004249:DNA Damage; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D000074443:Lysine Acetyltransferase 5; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009841:Oligonucleotides / Q000235:genetics; D034622:RNA Interference; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C569196:CCDC8 protein, human; D002352:Carrier Proteins; D046912:Multiprotein Complexes; D009841:Oligonucleotides; D016159:Tumor Suppressor Protein p53; D051548:Histone Acetyltransferases; C098489:KAT5 protein, human; D000074443:Lysine Acetyltransferase 5
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