title: Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter.
abstract: Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para-ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection.
authors.affiliation: The Rockefeller University, Laboratory of Chemical Biology and Microbial Pathogenesis, New York, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Grammel
authors.forename: Paul D
authors.initials: PD
authors.lastname: Dossa
authors.forename: Emma
authors.initials: E
authors.lastname: Taylor-Salmon
authors.forename: Howard C
authors.initials: HC
authors.lastname: Hang
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008718:Methionine-tRNA Ligase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020543:Proteome; D017459:Receptors, Amino Acid / Q000737:chemistry* / Q000378:metabolism; D012486:Salmonella typhimurium / Q000201:enzymology*
chemical_list: D020543:Proteome; D017459:Receptors, Amino Acid; C408243:p-ethynylphenylalanine; D008718:Methionine-tRNA Ligase; D000409:Alanine