title: Tax1-expressing feline 8C cells are useful to monitor the life cycle of human T-cell leukemia virus type I.
abstract: Extremely low infectivity has hampered direct (cell-free) infection studies of human T-cell leukemia virus type I (HTLV-I). In order to break through this barrier, we examined the susceptibility of many kinds of cells to HTLV-I and found a feline kidney cell line, 8C, that is highly susceptible to HTLV-I and produced remarkable amounts of infectious progeny viruses. Tax1 protein encoded by HTLV-I is known as a transcription activator for viral and cellular genes. We found that the 8C cells expressing the Tax1 protein (8C/TaxWT cells) can produce more progeny viruses than 8C cells when the cells were exposed to cell-free HTLV-I. A large number of syncytia were also induced in these cells. Here, we propose 8C/TaxWT cells as a useful tool to study the cell-free HTLV-I infection.
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Jinno-Oue
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Shinagawa
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Tokizawa
authors.affiliation: KAN Research Institute, Chuo-ku, Kobe 650-0047, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Akagi
authors.affiliation: Department of Virology and Preventive Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Hoshino
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D015870:Gene Expression; D016356:Gene Products, tax / Q000235:genetics / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development / Q000502:physiology*; D014773:Virology / Q000379:methods; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
chemical_list: D016356:Gene Products, tax; C514970:tax protein, Human T-lymphotrophic virus 1