title: Induced pluripotent stem cells from hair follicles as a cellular model for neurodevelopmental disorders.
abstract: Disease-specific induced pluripotent stem cells (iPSC) allow unprecedented experimental platforms for basic research as well as high-throughput screening. This may be particularly relevant for neuropsychiatric disorders, in which the affected neuronal cells are not accessible. Keratinocytes isolated from hair follicles are an ideal source of patients' cells for reprogramming, due to their non-invasive accessibility and their common neuroectodermal origin with neurons, which can be important for potential epigenetic memory. From a small number of plucked human hair follicles obtained from two healthy donors we reprogrammed keratinocytes to pluripotent iPSC. We further differentiated these hair follicle-derived iPSC to neural progenitors, forebrain neurons and functional dopaminergic neurons. This study shows that human hair follicle-derived iPSC can be differentiated into various neural lineages, suggesting this experimental system as a promising in vitro model to study normal and pathological neural developments, avoiding the invasiveness of commonly used skin biopsies.
authors.affiliation: INSERM U898, Nice, France.
authors.forename: I
authors.initials: I
authors.lastname: Petit
authors.forename: N Salman
authors.initials: NS
authors.lastname: Kesner
authors.forename: R
authors.initials: R
authors.lastname: Karry
authors.forename: O
authors.initials: O
authors.lastname: Robicsek
authors.forename: E
authors.initials: E
authors.lastname: Aberdam
authors.forename: F J
authors.initials: FJ
authors.lastname: Müller
authors.forename: D
authors.initials: D
authors.lastname: Aberdam
authors.forename: D
authors.initials: D
authors.lastname: Ben-Shachar
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005786:Gene Expression Regulation; D018859:Hair Follicle / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009422:Nervous System Diseases / Q000473:pathology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism