title: Induction of cell cycle arrest by the carbazole alkaloid Clauszoline-I from Clausena vestita D. D. Tao via inhibition of the PKCδ phosphorylation.
abstract: Sixteen carbazole alkaloids from Clausena vestita D. D. Tao were extracted, and their anti-tumor activities were evaluated. Among the extracts, Clauszoline-I exhibited an obvious growth inhibitory activity against several cancer cell lines through its ability to induce cell cycle arrest in the S and G2/M phases. A dramatic morphologic change with decreased F-actin staining and RhoA activity was found in Clauszoline-I treated HepG2 cells, in which the phosphorylation of PKCδ (Ser643) was inhibited. Our results indicated that induction cell cycle arrest by Clauszoline-I might be achieved by decreasing the RhoA activity via the inhibition of PKCδ phosphorylation.
authors.affiliation: Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Sisi
authors.initials: S
authors.lastname: Lin
authors.forename: Cuixian
authors.initials: C
authors.lastname: Li
authors.forename: Chun
authors.initials: C
authors.lastname: Zhou
authors.forename: Shaogui
authors.initials: S
authors.lastname: Wang
authors.forename: Heqing
authors.initials: H
authors.lastname: Huang
authors.forename: Peiqing
authors.initials: P
authors.lastname: Liu
authors.forename: Guan
authors.initials: G
authors.lastname: Ye
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Shen
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002227:Carbazoles / Q000737:chemistry* / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D054700:Clausena / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D051745:Protein Kinase C-delta / Q000737:chemistry / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D002227:Carbazoles; C572360:clauszoline-I; D012694:Serine; D051745:Protein Kinase C-delta; D020742:rhoA GTP-Binding Protein