title: Separation of small inorganic anions using methacrylate-based anion-exchange monolithic column prepared by low temperature UV photo-polymerization.
abstract: A methacrylate-based anion-exchange monolithic column was prepared by a single-step UV photo-copolymerization of [2-(methacryloyloxy)ethyl]-trimethyl ammonium chloride, butylmethacrylate, and ethylene dimethacrylate at a low temperature of -15 °C. The anion-exchange column exhibited good separation efficiency for the small inorganic anions of NO(2)(-), Br(-), NO(3)(-), and I(-) in the isocratic mode. Under the conditions optimized using a mobile phase of 50% ACN containing 100mM of NaCl, the theoretical plate heights of the anions were within the range of 12.2-15.6 μm (N, 64,000-82,000 m(-1); k, 0.2-1.6); and a value of 9.4 μm (N, 110,000 m(-1)) was achieved for t(0). The flow resistance of the column was acceptably low with a permeability of 2.7 × 10(-13) m(2). Fast gradient elution at a flow rate of 32 mm/s resulted in rapid and precise separation of the inorganic anions of IO(3)(-), NO(2)(-), Br(-), NO(3)(-), and I(-) within 20s.
authors.affiliation: Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya 466-8555, Japan.
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Hirano
authors.forename: Shinya
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohtani
mesh_terms: D000097:Acetonitriles; D000838:Anions / Q000032:analysis / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D055668:Photochemical Processes; D058105:Polymerization; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D000097:Acetonitriles; D000838:Anions; D008689:Methacrylates; C032159:acetonitrile