title: Gene delivery system involving Bubble liposomes and ultrasound for the efficient in vivo delivery of genes into mouse tongue tissue.
abstract: Oral squamous cell carcinoma is the most common type of head and neck cancer. Recently, efficient, easy, and minimally invasive gene delivery methods are expected to be developed as cancer gene therapies. However, the optimal method for delivering therapeutic genes into oral tissue for cancer treatment has not been elucidated. Therefore, we hypothesized that the tongue is a good target tissue for gene delivery with Bubble liposomes and ultrasound. To assess this, we attempted to deliver a mixture of plasmid DNA encoding a luciferase or enhanced green fluorescent protein, and Bubble liposomes into murine tongue with or without ultrasound exposure. The ultrasound conditions were 1 MHz, 2 W/cm(2), 60s, and duty cycle: 50%. The time-course of gene expression in the tongue was investigated with a luciferase assay and fluorescent microscopy. Luciferase expression was significantly increased in tongue transfected using Bubble liposomes and ultrasound compared with that of the tongue untreated with ultrasound, and this high level of luciferase activity was maintained for 2 weeks. From these results, Bubble liposomes can be used in combination with ultrasound to efficiently deliver plasmid DNA into the tongue in vivo. This technique is a highly promising approach for gene delivery into oral tissue.
authors.affiliation: Department of Periodontology, Showa University School of Dentistry, 2-1-1 Kitasenzoku, Ohta-ku, Tokyo 145-8515, Japan.
authors.forename: Marika
authors.initials: M
authors.lastname: Sugano
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Negishi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Endo-Takahashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Maruyama
authors.forename: Matsuo
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Aramaki
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000818:Animals; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008081:Liposomes; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D045423:Microbubbles; D008856:Microscopy, Fluorescence; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013997:Time Factors; D014059:Tongue / Q000378:metabolism*; D014465:Ultrasonics
chemical_list: C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D008081:Liposomes; D010714:Phosphatidylethanolamines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D015060:1,2-Dipalmitoylphosphatidylcholine; D011092:Polyethylene Glycols; D049409:Luciferases, Firefly