title: Six alkaloids inhibit secretion of IL-1α, TXB(2), ET-1 and E-selectin in LPS-induced endothelial cells.
abstract: The aim of the research was to investigate the antiendotoxin effects of Sinomenine, Fangchinoline, Stachydrine, Chuanxionggzine, Oxymartrine and Evodiamine. Endothelial cells were challenged with 1 μg/mL LPS for 3 h then treated respectively with six alkaloids at three concentrations (1, 5 and 10 μg/mL). The cells were incubated at 37°C in a cell incubator for 21 h. The supernatants were collected and analyzed the levels of interleukin-1α (IL-1α), thromboxane B(2) (TXB(2)), endothelin-1 (ET-1) and E-selectin by ELISA kits. The results revealed that Sinomenine, Oxymartrine and Evodiamine inhibited the production of IL-1α; Stachydrine, Chuanxionggzine and Evodiamine inhibited the secretion of TXB(2); Sinomenine and Oxymartrine down-regulated ET-1 expression; Fangchinoline and Evodiamine decreased the level of E-selectin. All these changes were significant. Taken together, the data suggested that six alkaloids may effectively reduce inflammatory response via these cytokines.
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Jiangsu Nanjing 210014, PR China. huyiyi6073@sina.com
authors.forename: Yi-Yi
authors.initials: YY
authors.lastname: Hu
authors.forename: Kong-Wang
authors.initials: KW
authors.lastname: He
authors.forename: Rong-li
authors.initials: RL
authors.lastname: Guo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal; D019040:E-Selectin / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000276:immunology; D032086:Evodia / Q000276:immunology; D006801:Humans; D053582:Interleukin-1alpha / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D009019:Morphinans / Q000494:pharmacology*; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology*; D011807:Quinolizines / Q000494:pharmacology*; D013552:Swine; D013929:Thromboxane B2 / Q000378:metabolism; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D019040:E-Selectin; D019332:Endothelin-1; D053582:Interleukin-1alpha; D008070:Lipopolysaccharides; D009019:Morphinans; D011392:Proline; D011719:Pyrazines; D011799:Quinazolines; D011807:Quinolizines; D013929:Thromboxane B2; C009271:sinomenine; C037573:oxymatrine; D000093842:Matrines; C060802:fangchinoline; C049639:evodiamine; C003342:stachydrine; C017953:tetramethylpyrazine