title: Crystallization and preliminary X-ray diffraction analysis of prion protein bound to the Fab fragment of the POM1 antibody.
abstract: Prion diseases are neurodegenerative diseases that are characterized by the conversion of the cellular prion protein PrP(c) to the pathogenic isoform PrP(sc). Several antibodies are known to interact with the cellular prion protein and to inhibit this transition. An antibody Fab fragment, Fab POM1, was produced that recognizes a structural motif of the C-terminal domain of mouse prion protein. To study the mechanism by which Fab POM1 recognizes and binds the prion molecule, the complex between Fab POM1 and the C-terminal domain of mouse prion (residues 120-232) was prepared and crystallized. Crystals of this binary complex belonged to the monoclinic space group C2, with unit-cell parameters a = 83.68, b = 106.9, c = 76.25 Å, β = 95.6°.
authors.affiliation: Department of Biochemistry, School of Molecular and Systems Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2P6, Canada.
authors.forename: Pravas Kumar
authors.initials: PK
authors.lastname: Baral
authors.forename: Barbara
authors.initials: B
authors.lastname: Wieland
authors.forename: Mridula
authors.initials: M
authors.lastname: Swayampakula
authors.forename: Magdalini
authors.initials: M
authors.lastname: Polymenidou
authors.forename: Adriano
authors.initials: A
authors.lastname: Aguzzi
authors.forename: Nat N V
authors.initials: NN
authors.lastname: Kav
authors.forename: Michael N G
authors.initials: MN
authors.lastname: James
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D011328:Prions / Q000737:chemistry* / Q000276:immunology
chemical_list: D007140:Immunoglobulin Fab Fragments; D011328:Prions
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