title: Simultaneous injection effective mixing analysis system for the determination of direct bilirubin in urinary samples.
abstract: A novel simultaneous injection effective mixing analysis system (SIEMA) for determination of direct bilirubin in urine sample was developed. Bilirubin reacts with diazotized sulfanilic acid in the presence of n-octyl-β-d-thioglucoside (OTG) as a solubilizing agent to form OTG-azobilirubin. The flow and chemical variables were investigated. A linear calibration graph for direct bilirubin was obtained over the range of 0-1.0 mg L(-1) (r(2)=0.994) with the limit of detection (3σ) of 4.7 μg L(-1), and the relative standard deviation (RSD) being 1.9% (n=11, 0.5 mg L(-1) of direct bilirubin). The results in healthy adult urine obtained by the proposed approach were found in good agreement with those obtained by the batch-wise diazo method.
authors.affiliation: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan.
authors.forename: Kraingkrai
authors.initials: K
authors.lastname: Ponhong
authors.forename: Norio
authors.initials: N
authors.lastname: Teshima
authors.forename: Kate
authors.initials: K
authors.lastname: Grudpan
authors.forename: Shoji
authors.initials: S
authors.lastname: Motomizu
authors.forename: Tadao
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000328:Adult; D001663:Bilirubin / Q000652:urine*; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006801:Humans; D012680:Sensitivity and Specificity; D012995:Solubility; D013425:Sulfanilic Acids / Q000737:chemistry; D013863:Thioglucosides / Q000737:chemistry; D016482:Urinalysis / Q000295:instrumentation
chemical_list: D013425:Sulfanilic Acids; D013863:Thioglucosides; C042989:n-octyl-beta-D-thioglucopyranoside; D001663:Bilirubin