title: Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species.
abstract: BAC FISH (fluorescence in situ hybridization using bacterial artificial chromosome probes) is a useful cytogenetic technique for physical mapping, chromosome marker screening, and comparative genomics. As a large genomic fragment with repetitive sequences is inserted in each BAC clone, random BAC FISH without adding competitive DNA can unveil complex chromosome organization of the repetitive elements in plants. Here we performed the comparative analysis of the random BAC FISH in monocot plants including species having small chromosomes (rice and asparagus) and those having large chromosomes (hexaploid wheat, onion, and spider lily) in order to understand a whole view of the repetitive element organization in Poales and Asparagales monocots. More unique and less dense dispersed signals of BAC FISH were observed in species with smaller chromosomes in both the Poales and Asparagales species. In the case of large-chromosome species, 75-85% of the BAC clones were detected as dispersed repetitive FISH signals along entire chromosomes. The BAC FISH of Lycoris did not even show localized repetitive patterns (e.g., centromeric localization) of signals.
authors.affiliation: Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan. gsuzuki@cc.osaka-kyoiku.ac.jp
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Ogaki
authors.forename: Nozomi
authors.initials: N
authors.lastname: Hokimoto
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Akie
authors.initials: A
authors.lastname: Kikuchi-Taura
authors.forename: Chiaki
authors.initials: C
authors.lastname: Harada
authors.forename: Ryozo
authors.initials: R
authors.lastname: Okayama
authors.forename: Asami
authors.initials: A
authors.lastname: Tsuru
authors.forename: Misa
authors.initials: M
authors.lastname: Onishi
authors.forename: Naoko
authors.initials: N
authors.lastname: Saito
authors.forename: Geum Sook
authors.initials: GS
authors.lastname: Do
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Takuro
authors.initials: T
authors.lastname: Ito
authors.forename: Akira
authors.initials: A
authors.lastname: Kanno
authors.forename: Maki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Mukai
mesh_terms: D027761:Asparagus Plant / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D059646:Genome Size / Q000235:genetics*; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D031426:Lycoris / Q000235:genetics; D019697:Onions / Q000235:genetics; D012275:Oryza / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D014908:Triticum / Q000235:genetics
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