title: Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples.
abstract: New technologies allow for genome-scale measurement of DNA methylation. In an effort to increase the clinical utility of DNA methylation as a biomarker, we have adapted a commercial bisulfite epigenotyping assay for genome-wide methylation profiling in archival formalin-fixed paraffin-embedded pathology specimens. This chapter takes the reader step by step through a biomarker discovery experiment to identify phenotype-correlated DNA methylation signatures in routine pathology specimens.
authors.affiliation: Cancer Genetics Branch, National Institutes of Health/National Cancer Institute, Bethesda, MD, USA.
authors.forename: J Keith
authors.initials: JK
authors.lastname: Killian
authors.forename: Robert L
authors.initials: RL
authors.lastname: Walker
authors.forename: Sven
authors.initials: S
authors.lastname: Bilke
authors.forename: Yidong
authors.initials: Y
authors.lastname: Chen
authors.forename: Sean
authors.initials: S
authors.lastname: Davis
authors.forename: Robert
authors.initials: R
authors.lastname: Cornelison
authors.forename: William I
authors.initials: WI
authors.lastname: Smith
authors.forename: Paul S
authors.initials: PS
authors.lastname: Meltzer
mesh_terms: D019175:DNA Methylation; D057890:Epigenomics / Q000379:methods*; D005785:Gene Conversion / Q000187:drug effects; D015894:Genome, Human; D006801:Humans; D016296:Mutagenesis / Q000187:drug effects; D016612:Paraffin Embedding; D013431:Sulfates / Q000009:adverse effects; D016707:Tissue Fixation
chemical_list: D013431:Sulfates; C012036:sodium sulfate