title: Analysis of Arabidopsis transcription factor families revealed extensive capacity for cell-to-cell movement as well as discrete trafficking patterns.
abstract: In plants, cell-to-cell communication is pivotal for the orchestration of cell fate determination, organ development, and the integration of whole plant physiology. One of the strategies for intercellular communication uses symplasmic communication channels, called plasmodesmata (PD). These PD establish unique cytoplasmic channels for the intercellular exchange not only of metabolites and small signaling molecules, but also of regulatory proteins and RNAs to allow for local orchestration of development and physiology. A number of non-cell-autonomous transcription factors (NCATFs) have been shown to function in the coordination of specific regulatory networks. To further explore the potential of such NCATFs, a genome-wide screen was performed on the transcription factor (TF) families in Arabidopsis. We here report that, among the 76 TFs examined, 22 were shown to move beyond their sites of transcription in the root apex; these NCATFs belonged to 17 TF families, including homeobox, GRAS, and MYB. Expression studies performed on variously-sized mCherry constructs identified a range of PD size exclusion limits within tissues of the root. In addition, our studies showed that actual protein level was an important factor controlling the range of TF intercellular movement. Interestingly, our studies on CAPRICE movement revealed tissue-specificity with respect to the mode of intercellular trafficking. These findings are discussed with respect to the regulation between cell-autonomous or non-cell-autonomous action.
authors.affiliation: Division of Applied Life Science (BK21/WCU program), Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Yeonggil
authors.initials: Y
authors.lastname: Rim
authors.forename: Lijun
authors.initials: L
authors.lastname: Huang
authors.forename: Hyosub
authors.initials: H
authors.lastname: Chu
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong-Chan
authors.initials: JC
authors.lastname: Hong
authors.forename: William J
authors.initials: WJ
authors.lastname: Lucas
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002450:Cell Communication; D018929:Cell Culture Techniques; D018519:Meristem / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D021381:Protein Transport; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors
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