title: In vitro selection of unnatural cyclic peptide libraries via mRNA display.
abstract: The ribosomal synthesis of drug-like peptides containing unnatural amino acids is possible due to the broad substrate specificity of the ribosome. In this protocol, a reconstituted Escherichia coli ribosomal translation system (PURE) is adapted to incorporate unnatural amino acids into mRNA-displayed peptide libraries, which are used in in vitro selection.
authors.affiliation: Department of Chemistry, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Ma
authors.forename: Matthew C T
authors.initials: MC
authors.lastname: Hartman
mesh_terms: D000596:Amino Acids / Q000235:genetics; D019020:Directed Molecular Evolution / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics
chemical_list: D000596:Amino Acids; D019151:Peptide Library; D010456:Peptides, Cyclic; D012333:RNA, Messenger