title: Integration of nanoparticle cell lysis and microchip PCR for one-step rapid detection of bacteria.
abstract: This paper describes an integrated microchip system as an efficient and cost-effective solution involving Nanotechnology and Lab-on-a-Chip technology for the rapid detection of bacteria. The system is based on using surface-modified gold nanoparticles for efficient cell lysis followed by microchip PCR without having to remove the nanoparticles from the PCR solution. Poly(quaternary ammonium) modified gold nanoparticles are used to provide a novel and efficient cell lysis method without the need to go through time-consuming, expensive and complicated microfabrication processes as most of current cell lysis methods for Lab-on-a-Chip applications do. It also facilitates the integration of cell lysis and PCR by sharing the same reaction chamber as PCR uses. It is integrated with a prototype microchip PCR system consisting of a physical microchip PCR device and an automated temperature control mechanism. The research work explores solutions for the problem of PCR inhibition caused by gold nanoparticles as well as for the problem of non-specific PCR amplification in the integrated microchip system. It also explores the possibility of greatly reducing PCR cycling time to achieve the same result compared to the protocol for a regular PCR machine. The simplicity of the setup makes it easy to be integrated with other Lab-on-a-Chip functional modules to create customized solutions for target applications.
authors.affiliation: Department of Systems Design Engineering, University of Waterloo, Waterloo N2L 3G1, Canada.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wan
authors.forename: John T W
authors.initials: JT
authors.lastname: Yeow
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006046:Gold; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry*; D046208:Microchip Analytical Procedures / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
chemical_list: D006046:Gold