title: Molecular identification of Fusarium species in Gibberella fujikuroi species complex from rice, sugarcane and maize from Peninsular Malaysia.
abstract: The objective of this study was to identify Fusarium species in the Gibberella fujikuroi species complex from rice, sugarcane and maize as most of the Fusarium species in the species complex are found on the three crops. Isolates used were collected from the field and obtained from culture collection. The Fusarium isolates were initially sorted based on morphology and identifications confirmed based on the DNA sequence of the translation elongation factor 1-α (TEF-1α) gene. Based on the closest match of BLAST analysis, five species were recovered, namely, F. sacchari, F. fujikuroi, F. proliferatum, F. andiyazi and F. verticillioides. This is the first report regarding F. andiyazi from rice in Malaysia and Southeast Asia. The phylogenetic tree generated by using the neighbor joining method showed that isolates from the same species were grouped in the same clade. The present study indicated that Fusarium species in the G. fujikuroi species complex are widespread in rice, sugarcane and maize in Peninsular Malaysia. The findings also suggest that the use of morphological characters for identification of Fusarium species in the G. fujikuroi species complex from the three crops will lead to incorrect species designation.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia; E-Mails:  hmsuan@yahoo.com  (H.M.H.);  sallehb@usm.my  (B.S.).
authors.forename: Heng Mei
authors.initials: HM
authors.lastname: Hsuan
authors.forename: Baharuddin
authors.initials: B
authors.lastname: Salleh
authors.forename: Latiffah
authors.initials: L
authors.lastname: Zakaria
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005670:Fusarium / Q000145:classification / Q000235:genetics*; D005874:Gibberella / Q000145:classification / Q000235:genetics; D008296:Malaysia; D012275:Oryza / Q000145:classification / Q000235:genetics; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D031786:Saccharum / Q000145:classification / Q000235:genetics; D003313:Zea mays / Q000145:classification / Q000235:genetics
chemical_list: D020648:Peptide Elongation Factor 1; D010940:Plant Proteins; D004247:DNA
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