title: Combined age- and trauma-related proteomic changes in rat neocortex: a basis for brain vulnerability.
abstract: This proteomic study investigates the widely observed clinical phenomenon, that after comparable brain injuries, geriatric patients fare worse and recover less cognitive and neurologic function than younger victims. Utilizing a rat traumatic brain injury model, sham surgery or a neocortical contusion was induced in 3 age groups. Geriatric (21 months) rats performed worse on behavioral measures than young adults (12-16 weeks) and juveniles (5-6 weeks). Motor coordination and certain cognitive deficits showed age-dependence both before and after injury. Brain proteins were analyzed using silver-stained two-dimensional electrophoresis gels. Spot volume changes (>2-fold change, p<0.01) were identified between age and injury groups using computer-assisted densitometry. Sequences were determined by mass spectrometry of tryptic peptides. The 19 spots identified represented 13 different genes that fell into 4 general age- and injury-dependent expression patterns. Fifteen isoforms changed differentially with respect to both age and injury (p<0.05). Further investigations into the nature and function of these isoforms may yield insights into the vulnerability of older patients and resilience of younger patients in recovery after brain injuries.
authors.affiliation: University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0517, USA.
authors.forename: Neal D
authors.initials: ND
authors.lastname: Mehan
authors.forename: Kenneth I
authors.initials: KI
authors.lastname: Strauss
mesh_terms: D000367:Age Factors; D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D001930:Brain Injuries / Q000150:complications / Q000473:pathology*; D003072:Cognition Disorders / Q000175:diagnosis / Q000209:etiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D018782:Maze Learning / Q000502:physiology; D009043:Motor Activity / Q000502:physiology; D019579:Neocortex / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009422:Nervous System Diseases / Q000209:etiology; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009419:Nerve Tissue Proteins
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