title: Involvement of the putative Ca²⁺-permeable mechanosensitive channels, NtMCA1 and NtMCA2, in Ca²⁺ uptake, Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in tobacco (Nicotiana tabacum) BY-2 cells.
abstract: To gain insight into the cellular functions of the mid1-complementing activity (MCA) family proteins, encoding putative Ca²⁺-permeable mechanosensitive channels, we isolated two MCA homologs of tobacco (Nicotiana tabacum) BY-2 cells, named NtMCA1 and NtMCA2. NtMCA1 and NtMCA2 partially complemented the lethality and Ca²⁺ uptake defects of yeast mutants lacking mechanosensitive Ca²⁺ channel components. Furthermore, in yeast cells overexpressing NtMCA1 and NtMCA2, the hypo-osmotic shock-induced Ca²⁺ influx was enhanced. Overexpression of NtMCA1 or NtMCA2 in BY-2 cells enhanced Ca²⁺ uptake, and significantly alleviated growth inhibition under Ca²⁺ limitation. NtMCA1-overexpressing BY-2 cells showed higher sensitivity to hypo-osmotic shock than control cells, and induced the expression of the touch-inducible gene, NtERF4. We found that both NtMCA1-GFP and NtMCA2-GFP were localized at the plasma membrane and its interface with the cell wall, Hechtian strands, and at the cell plate and perinuclear vesicles of dividing cells. NtMCA2 transcript levels fluctuated during the cell cycle and were highest at the G1 phase. These results suggest that NtMCA1 and NtMCA2 play roles in Ca²⁺-dependent cell proliferation and mechanical stress-induced gene expression in BY-2 cells, by regulating the Ca²⁺ influx through the plasma membrane.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Kurusu
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Masataka
authors.initials: M
authors.lastname: Nakano
authors.forename: Akiko
authors.initials: A
authors.lastname: Takiguchi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ogasawara
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Iida
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hanamata
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Iida
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Kuchitsu
mesh_terms: D001692:Biological Transport / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D040542:Mechanotransduction, Cellular / Q000235:genetics*; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D002118:Calcium
references.citation: Plant Cell Physiol. 2000 Nov;41(11):1187-92
references.pmid: 11092902
references.citation: Plant Cell Physiol. 2011 Nov;52(11):1915-30
references.pmid: 21949028
references.citation: Plant Physiol. 2010 Jun;153(2):678-92
references.pmid: 20357140
references.citation: Mol Cell Biol. 1997 Nov;17(11):6339-47
references.pmid: 9343395
references.citation: Annu Rev Plant Biol. 2010;61:593-620
references.pmid: 20192754
references.citation: J Biol Chem. 2003 Mar 14;278(11):9647-54
references.pmid: 12514173
references.citation: FEBS Lett. 2006 Jan 23;580(2):597-602
references.pmid: 16405971
references.citation: Genetics. 2001 Dec;159(4):1527-38
references.pmid: 11779794
references.citation: Plant Physiol. 2003 Jun;132(2):728-31
references.pmid: 12805601
references.citation: Nature. 1991 Aug 8;352(6335):524-6
references.pmid: 1865907
references.citation: Ann Bot. 2003 Oct;92(4):487-511
references.pmid: 12933363
references.citation: Plant Physiol. 2004 Jun;135(2):1027-39
references.pmid: 15181212
references.citation: Curr Biol. 2006 Jun 6;16(11):1147-53
references.pmid: 16753570
references.citation: Plant Physiol. 2007 May;144(1):402-18
references.pmid: 17337521
references.citation: Plant Physiol. 2008 Feb;146(2):505-14
references.pmid: 18055589
references.citation: Science. 1999 Aug 6;285(5429):882-6
references.pmid: 10436155
references.citation: Protoplasma. 2010 Dec;247(3-4):215-31
references.pmid: 20658253
references.citation: Plant Physiol. 1998 Mar;116(3):879-90
references.pmid: 9501121
references.citation: Oncogene. 2005 Nov 17;24(51):7524-41
references.pmid: 16170375
references.citation: New Phytol. 2005 Feb;165(2):373-89
references.pmid: 15720650
references.citation: J Plant Res. 2011 May;124(3):315-24
references.pmid: 21409543
references.citation: Plant Physiol. 1985 Oct;79(2):568-70
references.pmid: 16664453
references.citation: FEBS Lett. 1997 Dec 15;419(2-3):259-62
references.pmid: 9428646
references.citation: Ann Bot. 2010 Jun;105(7):1081-102
references.pmid: 20410048
references.citation: FEBS Lett. 1997 Jun 9;409(2):166-70
references.pmid: 9202139
references.citation: Plant Cell Physiol. 2005 Jan;46(1):156-65
references.pmid: 15659447
references.citation: Gene. 2003 May 8;309(2):81-9
references.pmid: 12758124
references.citation: J Biol Chem. 1990 Aug 5;265(22):13391-9
references.pmid: 2198292
references.citation: Plant Physiol. 1978 Dec;62(6):949-53
references.pmid: 16660646
references.citation: Gene. 1988 Dec 30;74(2):527-34
references.pmid: 3073106
references.citation: Plant Physiol. 2000 Apr;122(4):1365-77
references.pmid: 10759533
references.citation: Plant Mol Biol. 2006 Dec;62(6):867-80
references.pmid: 16941207
references.citation: Plant Cell. 2002;14 Suppl:S401-17
references.pmid: 12045291
references.citation: Plant Cell Physiol. 2004 Jun;45(6):693-702
references.pmid: 15215504
references.citation: Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6878-82
references.pmid: 1862111
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3639-44
references.pmid: 17360695
references.citation: Plant Physiol. 2010 Mar;152(3):1284-96
references.pmid: 20097794
references.citation: Plant J. 2009 Jan;57(1):55-64
references.pmid: 18778403
references.citation: Plant Sci. 2001 Feb 5;160(3):381-404
references.pmid: 11166425
references.citation: Curr Biol. 2008 May 20;18(10):730-734
references.pmid: 18485707
references.citation: Plant Physiol. 2004 Jan;134(1):59-66
references.pmid: 14657406
references.citation: FEMS Microbiol Lett. 2007 Jun;271(1):48-52
references.pmid: 17391362
references.citation: Plant Cell Physiol. 2001 Jan;42(1):74-84
references.pmid: 11158446
references.citation: J Cell Biol. 1985 May;100(5):1363-8
references.pmid: 3921550
references.citation: J Cell Biol. 1990 Jul;111(1):171-82
references.pmid: 2114409
references.citation: Nature. 2008 Mar 27;452(7186):487-91
references.pmid: 18305484
references.citation: Methods Mol Biol. 1996;53:139-45
references.pmid: 8924976
references.citation: Mol Cell Biol. 1994 Dec;14(12):8259-71
references.pmid: 7526155
references.citation: Trends Plant Sci. 2002 May;7(5):193-5
references.pmid: 11992820
references.citation: Plant Cell Physiol. 2004 Apr;45(4):496-500
references.pmid: 15111725
references.citation: J Plant Growth Regul. 2002 Jun;21(2):71-88
references.pmid: 12016507
references.citation: Trends Cell Biol. 2009 Apr;19(4):180-8
references.pmid: 19285867
references.citation: Mol Cell Biol. 2006 May;26(9):3401-13
references.pmid: 16611984