title: In vitro activity of Paris polyphylla smith against enterovirus 71 and coxsackievirus B3 and its immune modulation.
abstract: Enterovirus 71 (EV71) and coxsackievirus B3 (CVB3) have resulted in severe pathogenesis caused by the host's immune response, including the cytokine cascade. Paris polyphylla Smith is a folk medicinal plant in Asia traditionally prescribed for the reduction of pain and elimination of poisoning. In this study, we investigated the anti-EV71 and CVB3 activity of P. polyphylla Smith as well as its immune modulation. The IC(50) for the P. polyphylla Smith 95% ethanol extract against EV71 and CVB3 were 12.5-23% and 99-156% of that of ribavirin, a positive control. Prevention of viral infection, viral inactivation, and anti-viral replication effects against both EV71 and CVB3 were demonstrated by the extract, the anti-viral replication effect being dominant. The extract significantly increased IL-6 production in both EV71- and CVB3-infected cells. A high correlation was possibly demonstrated between the high amounts of IL-6 induction in the EV71 and CVB3-infected cells and the anti-viral replication activity of the extract. In conclusion, good anti-EV71 and CVB3 activity was observed in the P. polyphylla Smith 95% ethanol extract. The high amounts of IL-6 induction in the virus-infected cells played a key role in the anti-viral activity of the extract.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan. ycwang@nchu.edu.tw
authors.forename: Yuan-Chuen
authors.initials: YC
authors.lastname: Wang
authors.forename: Tsu-Yi
authors.initials: TY
authors.lastname: Yi
authors.forename: Kuei-Hsiang
authors.initials: KH
authors.lastname: Lin
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003384:Coxsackievirus Infections / Q000276:immunology / Q000821:virology*; D016207:Cytokines / Q000276:immunology; D029821:Enterovirus A, Human / Q000187:drug effects* / Q000502:physiology; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D019668:Liliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D016207:Cytokines; D007155:Immunologic Factors; D010936:Plant Extracts