title: Assessing the genotoxic potentials of roxarsone in V79 cells using the alkaline Comet assay and micronucleus test.
abstract: Until recently, knowledge about the genotoxicity of roxarsone in vitro or in vivo was limited. This study assessed the genotoxicity of roxarsone in an in vitro system. Roxarsone was tested for potential genotoxicity on V79 cells by a Comet assay and a micronucleus (MN) test, exposing the cells to roxarsone (1-500 μM) and to sodium arsenite (NaAsO₂, 20 μM) solutions for 3-48 h. Roxarsone was found to be cytotoxic when assessed with a commercial cell counting kit (CCK-8) used to evaluate cell viability, and moderately genotoxic in the Comet assay and micronucleus test used to assess DNA damage. The Comet metrics (percentages TDNA, TL, TM) increased significantly in a time- and concentration-dependent manner in roxarsone-treated samples compared with PBS controls (P<0.05), while the data from samples treated with 20 μM NaAsO₂ were comparable to those from 500 μM roxarsone-treated samples. The MN frequency of V79 cells treated with roxarsone was higher than that in the negative control but lower than the frequency in cells treated with 20 μM NaAsO₂. A dose- and time-dependent response in MN induction was observed at 10, 50, 100 and 500 μM doses of roxarsone after 12-48 h exposure time. The DNA damage in V79 cells treated with 500 μM roxarsone was similar to cells exposed to 20 μM NaAsO₂. The uptake of cells was correlated with the DNA damage caused by roxarsone. This investigation depicts the genotoxic potentials of roxarsone to V79 cells, which could lead to further advanced studies on the genotoxicity of roxarsone.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Yangzhou University, Jiangsu, 225009, PR China. ymzhnet@sina.com.cn
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Chenyun
authors.initials: C
authors.lastname: Hu
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity; D002460:Cell Line; D003049:Coccidiostats / Q000633:toxicity*; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D012406:Roxarsone / Q000633:toxicity*; D017670:Sodium Compounds / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D018053:Arsenites; D003049:Coccidiostats; D009153:Mutagens; D017670:Sodium Compounds; C017947:sodium arsenite; D012406:Roxarsone