title: A simple synthesis of APM ([p-(N-acrylamino)-phenyl]mercuric chloride), a useful tool for the analysis of thiolated biomolecules.
abstract: This study describes two novel synthetic procedures to prepare APM, a useful tool for the analysis and the purification of thiolated biomolecules. The methods developed are technically simple and robust and allowed the first full characterization of pure APM. Moreover, the efficacy of APM, as a biochemical tool, was demonstrated by analysis of tRNA thiolation by APM-PAGE.
authors.affiliation: Laboratoire de Chimie Bio-Organique, Unité de Chimie Organique (UCO), University of Namur (FUNDP), Rue de Bruxelles 61, B-5000 Namur, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Lemau de Talancé
authors.forename: F
authors.initials: F
authors.lastname: Bauer
authors.forename: D
authors.initials: D
authors.lastname: Hermand
authors.forename: S P
authors.initials: SP
authors.lastname: Vincent
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D009941:Organomercury Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010663:Phenylmercury Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012343:RNA, Transfer / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C570580:((p-(N-acrylamino)phenyl)mercuric chloride); D000178:Acrylamides; D009941:Organomercury Compounds; D010663:Phenylmercury Compounds; D013438:Sulfhydryl Compounds; D012343:RNA, Transfer; C008859:phenylmercuric chloride