title: Iodine concentration and signs of apoptosis in the thyroid and pituitary of female rats after different single doses of potassium iodide.
abstract: OBJECTIVE
  The aim of this work was to study the content of iodine as well as the expression of caspase 8 and caspase 32 in the thyroid and anterior pituitary in rats after a single dose of iodide.
  
  
  METHODS
  A total of 49 inbred rat females weighing 250-300 g at the stage of diestrus and/or metestrus were used. Pituitaries and thyroids were dissected from 15 control rats and from the groups of 8 rats each given potassium iodide by gavage in doses of 1, 4, 8 and 25 μg/100 g at 48 h before sacrifice. In two rats of each group the level of iodine in thyroids and pituitaries was estimated in terms of weight percent of iodide in dry tissue (wt % I-2/dry tissue) using the wavelength dispersive spectrometry (WDS) quantitative analysis. The expression of caspase 8 and caspase 32 in thyroids and pituitaries in terms of the percentage of positive immunostained area (% PA) was measured by streptavidin-biotin method using specific polyclonal antibodies. RESULTS; In the thyroids, iodine concentration increased after 1 μg/100 g, but decreased after 8 and 25 μg/100 g, while that in the pituitaries significantly increased after all doses of iodide with the peak after 8 mg/100 g. After the same iodide dose also the peak of caspase 32 and caspase 8 appeared in the pituitary. However, in the thyroid only increased caspase 32 was found together with a decrease of iodine concentration.
  
  
  CONCLUSIONS
  Several interrelations between iodine in the thyroid and pituitary were found. In addition, the signs of apoptosis appeared directly related to the concentration of iodine in the pituitary, but inversely related to iodine concentration in the thyroid.
authors.affiliation: The Territorial Branch of Russian Railways State-Owned Joint Stock Company, Chelyabinsk, Russia. nadyabas@gmail.com
authors.forename: N L
authors.initials: NL
authors.lastname: Basalaeva
authors.forename: G V
authors.initials: GV
authors.lastname: Sychugov
authors.forename: V K
authors.initials: VK
authors.lastname: Strizhikov
authors.forename: E N
authors.initials: EN
authors.lastname: Mikhailova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053181:Caspase 8 / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007455:Iodine / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010902:Pituitary Gland / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D011193:Potassium Iodide / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D011193:Potassium Iodide; D007455:Iodine; C505528:Casp8 protein, rat; D053181:Caspase 8; D020169:Caspases; C554639:caspase 32, rat