title: Response of human bone marrow stromal cells, MG-63, and SaOS-2 to titanium-based dental implant surfaces with different topography and surface energy.
abstract: OBJECTIVE
  Osseointegration is dependent on different parameters of the implant surface like surface roughness and physicochemical properties. In vitro studies using a wide variety of surface parameters and cell lines make it difficult to address the influence of a single parameter. With this study the influence of surface topography and energy on different osteoblast derived cell lines, namely MG-63 and SaOS-2 and of human mesenchymal stromal cells (hMSC) were investigated.
  
  
  METHODS
  Cells were cultured on polished (POL) and sandblasted/hot acid etched (SBA) titanium surfaces which were partly alkaline treated (SBA NaOH). Cell morphology, metabolic activity, tissue non-specific alkaline phosphatase (TNAP) activity and prostaglandin E(2)  (PGE(2) ) formation were determined.
  
  
  RESULTS
  Impaired spreading was found on both SBA surfaces. Proliferation after 4 and 7 days increased on POL compared to both SBA surfaces. TNAP activity of hMSC and MG-63 was increased on POL compared to both SBA surfaces whereas SaOS-2 did not discriminate between the three surfaces. PGE(2)  formation of hMSC and MG-63 was on both SBA surfaces after 2 days significantly higher than on POL.
  
  
  CONCLUSIONS
  The results of this study show that surface roughness has a distinct influence on proliferation and differentiation of osteoblasts. However, variations in physicochemical properties seem to have little influence under the used experimental conditions. It is suggested that more sever and long-lasting modifications of surface chemistry would have an influence on osteoblastic cells.
authors.affiliation: Institute of Physiological Chemistry, University of Technology, Dresden, Germany. ute.hempel@tu-dresden.de
authors.forename: Ute
authors.initials: U
authors.lastname: Hempel
authors.forename: Thomas
authors.initials: T
authors.lastname: Hefti
authors.forename: Peter
authors.initials: P
authors.lastname: Dieter
authors.forename: Falko
authors.initials: F
authors.lastname: Schlottig
mesh_terms: D000134:Acid Etching, Dental; D000469:Alkaline Phosphatase / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D015921:Dental Implants; D017267:Dental Prosthesis Design; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D013194:Staining and Labeling; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D015921:Dental Implants; D004247:DNA; D014025:Titanium; D000469:Alkaline Phosphatase