title: Complete sequence of a cryptic virus from hemp (Cannabis sativa).
abstract: Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus. The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained.
authors.affiliation: Institute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany. angelika.ziegler@jki.bund.de
authors.forename: Angelika
authors.initials: A
authors.lastname: Ziegler
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Matoušek
authors.forename: Gerhard
authors.initials: G
authors.lastname: Steger
authors.forename: Jörg
authors.initials: J
authors.lastname: Schubert
mesh_terms: D002188:Cannabis / Q000821:virology*; D017788:Carlavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral