title: Characterization of supported lipid bilayer disruption by chrysophsin-3 using QCM-D.
abstract: Antimicrobial peptides (AMPs) are naturally occurring polymers that can kill bacteria by destabilizing their membranes. A quartz crystal microbalance with dissipation monitoring (QCM-D) was used to better understand the action of the AMP chrysophsin-3 on supported lipid bilayers (SLB) of phosphatidylcholine. Interaction of the SLB with chrysophsin-3 at 0.05 μM demonstrated changes in frequency (Δf) and energy dissipation (ΔD) that were near zero, indicating little change in the membrane. At higher concentrations of chyrsophsin-3 (0.25-4 μM), decreases in Δf of up to 7 Hz were measured. These negative frequency changes suggest that mass was being added to the SLB, possibly due to peptide insertion into the membrane. At a chrysophsin-3 concentration of 10 μM, there was a net mass loss, which was attributed to pore formation in the membrane. QCM-D can be used to describe a mechanistic relationship between AMP concentration and interaction with a model cell membrane.
authors.affiliation: Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA.
authors.forename: Kathleen F
authors.initials: KF
authors.lastname: Wang
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Nagarajan
authors.forename: Charlene M
authors.initials: CM
authors.lastname: Mello
authors.forename: Terri A
authors.initials: TA
authors.lastname: Camesano
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008958:Models, Molecular; D010713:Phosphatidylcholines / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques; D013816:Thermodynamics
chemical_list: D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C472594:chrysophsin