title: [Study on pharmacokinetics of borneol in rats injected with novel-xingnaojing by GC-FID].
abstract: OBJECTIVE
  To develop a GC-FID method for the determination of borneol concentration in rat plasma and to investigate the pharmacokinetics after injection of novel-Xingnaojing.
  
  
  METHODS
  Novel-Xingnaojing was injected via by caudal vein injection. The blood samples were collected by posterior orbital venous plexus approach at 0.5, 1, 3, 5, 8, 12, 20, 30, 45 min. The drug in plasma was extracted with ethyl acetate and then detected by GC-FID, octadecane was used as the internal standard. The pharmacokinetic parameters were calculated by the software of Kinetica.
  
  
  RESULTS
  The calibration curve was good linear in the range of 1.67-16.67 mg x L(-1). The extraction recoveries of low, medium and high concentration were (92.81 +/- 1.11)%, (85.38 +/- 0.86)% and (84.58 +/- 0.58)%, respectivley. And the RSDs of within-day and between-day were below 3.00%. Plasma concentration of borneol was consistent with the two-compartment open model. The pharmacokinetic parameters were that the t1/2alpha was (1.18 +/- 0.20) min, the t1/2beta was (22.27 +/- 6.85) min, the C(max)(Calc) was (18.76 +/- 2.10) mg x L(-1), the MRT was (23.84 +/- 7.67) min(-1), and the AUC was (100.00 +/- 15.85) mg x min x L(-1).
  
  
  CONCLUSIONS
  The GC-FID method developed can be applied to determination and pharmacokinetics. The borneol in novel-Xingnaojing is distributed and metabolized fast after being administrated.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China. landocean28@163.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Chen
authors.forename: Pengyue
authors.initials: P
authors.lastname: Li
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Zhai
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Dongxue
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D001892:Camphanes / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005410:Flame Ionization / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001892:Camphanes; D004365:Drugs, Chinese Herbal; C022871:isoborneol