title: Tailoring material properties of a nanofibrous extracellular matrix derived hydrogel.
abstract: In the native tissue, the interaction between cells and the extracellular matrix (ECM) is essential for cell migration, proliferation, differentiation, mechanical stability, and signaling. It has been shown that decellularized ECMs can be processed into injectable formulations, thereby allowing for minimally invasive delivery. Upon injection and increase in temperature, these materials self-assemble into porous gels forming a complex network of fibers with nanoscale structure. In this study we aimed to examine and tailor the material properties of a self-assembling ECM hydrogel derived from porcine myocardial tissue, which was developed as a tissue specific injectable scaffold for cardiac tissue engineering. The impact of gelation parameters on ECM hydrogels has not previously been explored. We examined how modulating pH, temperature, ionic strength, and concentration affected the nanoscale architecture, mechanical properties, and gelation kinetics. These material characteristics were assessed using scanning electron microscopy, rheometry, and spectrophotometry, respectively. Since the main component of the myocardial matrix is collagen, many similarities between the ECM hydrogel and collagen gels were observed in terms of the nanofibrous structure and modulation of properties by altering ionic strength. However, variation from collagen gels was noted for the gelation temperature along with varied times and rates of gelation. These discrepancies when compared to collagen are likely due to the presence of other ECM components in the decellularized ECM based hydrogel. These results demonstrate how the material properties of ECM hydrogels could be tailored for future in vitro and in vivo applications.
authors.affiliation: Department of Bioengineering, University of California, San Diego 9500 Gilman Drive MC 0412, La Jolla, CA 92093-0412, USA.
authors.forename: Todd D
authors.initials: TD
authors.lastname: Johnson
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Lin
authors.forename: Karen L
authors.initials: KL
authors.lastname: Christman
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009994:Osmolar Concentration; D012212:Rheology; D013552:Swine; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels
references.citation: J Biomech. 2010 Feb 10;43(3):500-5
references.pmid: 19880123
references.citation: J Am Coll Cardiol. 2006 Sep 5;48(5):907-13
references.pmid: 16949479
references.citation: Biomaterials. 1996 Feb;17(3):291-9
references.pmid: 8745326
references.citation: J Cell Mol Med. 2010 May;14(5):1044-55
references.pmid: 20193036
references.citation: J Biomech Eng. 2002 Apr;124(2):214-22
references.pmid: 12002131
references.citation: Tissue Eng Part B Rev. 2009 Jun;15(2):171-93
references.pmid: 19207040
references.citation: Biophys J. 2008 Mar 15;94(6):2361-73
references.pmid: 18065452
references.citation: Biomaterials. 2003 Oct;24(22):4001-9
references.pmid: 12834595
references.citation: J Biomech. 2003 Oct;36(10):1529-53
references.pmid: 14499302
references.citation: Acta Biomater. 2009 Jan;5(1):1-13
references.pmid: 18938117
references.citation: Biomaterials. 2009 Oct;30(29):5409-16
references.pmid: 19608268
references.citation: Biomaterials. 2006 Jun;27(16):3136-46
references.pmid: 16473404
references.citation: Biomaterials. 1999 Mar;20(6):573-88
references.pmid: 10213360
references.citation: Biophys J. 2009 Feb 18;96(4):1566-85
references.pmid: 19217873
references.citation: Biomaterials. 2003 Nov;24(24):4337-51
references.pmid: 12922147
references.citation: Laryngoscope. 1998 May;108(5):725-31
references.pmid: 9591554
references.citation: Biochem J. 1960 Jun;75:588-98
references.pmid: 13845809
references.citation: Nat Biotechnol. 2005 Jan;23(1):47-55
references.pmid: 15637621
references.citation: Biomaterials. 2008 Apr;29(11):1630-7
references.pmid: 18201760
references.citation: J Endourol. 1994 Apr;8(2):125-30
references.pmid: 8061669
references.citation: Micron. 2007;38(5):513-21
references.pmid: 17045806
references.citation: Acta Biomater. 2011 Mar;7(3):1040-9
references.pmid: 20932943
references.citation: Eur J Cancer. 1995;31A(3):375-80
references.pmid: 7540403
references.citation: Am J Physiol Heart Circ Physiol. 2005 Sep;289(3):H973-4
references.pmid: 16100252
references.citation: Int J Pharm. 2001 Jun 19;221(1-2):1-22
references.pmid: 11397563
references.citation: Biomacromolecules. 2003 May-Jun;4(3):713-22
references.pmid: 12741789
references.citation: Nat Med. 2008 Feb;14(2):213-21
references.pmid: 18193059
references.citation: Biomaterials. 2007 Sep;28(25):3587-93
references.pmid: 17524477
references.citation: Pharmacol Ther. 2005 Feb;105(2):151-63
references.pmid: 15670624
references.citation: Macromol Biosci. 2011 Jun 14;11(6):731-8
references.pmid: 21322109
references.citation: Biomaterials. 1994 Oct;15(12):985-95
references.pmid: 7841296
references.citation: Tissue Eng Part A. 2010 Jun;16(6):2017-27
references.pmid: 20100033
references.citation: J Cardiovasc Transl Res. 2010 Oct;3(5):478-86
references.pmid: 20632221
references.citation: Biopolymers. 2000 Sep;54(3):222-34
references.pmid: 10861383
references.citation: Biomacromolecules. 2008 Apr;9(4):1106-16
references.pmid: 18327908
references.citation: Acta Biomater. 2011 Jan;7(1):1-15
references.pmid: 20619368
references.citation: J Cell Biol. 2004 Sep 13;166(6):877-87
references.pmid: 15364962
references.citation: Adv Drug Deliv Rev. 2007 Dec 10;59(14):1413-33
references.pmid: 17916396
references.citation: J Biomed Mater Res. 2002 Jun 15;60(4):613-21
references.pmid: 11948520
references.citation: FASEB J. 1999;13 Suppl:S3-15
references.pmid: 10352140
references.citation: J Mol Biol. 2008 Mar 7;376(5):1509-22
references.pmid: 18234220
references.citation: J Biol Chem. 1979 Jan 10;254(1):180-6
references.pmid: 758319
references.citation: J Biol Chem. 1978 Sep 25;253(18):6578-85
references.pmid: 28330
references.citation: Bull Exp Biol Med. 2010 Dec;150(1):153-6
references.pmid: 21161075
references.citation: PLoS One. 2010 Sep 27;5(9):e13039
references.pmid: 20885963
references.citation: J Biochem. 1974 Jul;76(1):177-86
references.pmid: 4612027