title: Modified gold surfaces by poly(amidoamine) dendrimers and fructose dehydrogenase for mediated fructose sensing.
abstract: An electrochemical biosensor for detection of fructose in food samples was developed by immobilization of fructose dehydrogenase (FDH) on cysteamine and poly(amidoamine) dendrimers (PAMAM)-modified gold electrode surface. Electrochemical analysis was carried out by using hexacyanoferrate (HCF) as a mediator and the response time was 35s at +300 mV vs. Ag/AgCl. Moreover, some parameters such as pH, enzyme loading and type of PAMAM (Generations 2, 3 and 4) were investigated. Then, the FDH biosensor was calibrated for fructose in the concentration range of 0.25-5.0mM. To evaluate its utility, the FDH biosensor was applied for fructose analysis in real samples. Finally, obtained data were compared with those measured with HPLC as a reference method.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department 35100 Bornova, Izmir, Turkiye.
authors.forename: Kadir
authors.initials: K
authors.lastname: Damar
authors.forename: Dilek
authors.initials: D
authors.lastname: Odaci Demirkol
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000191:economics / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005632:Fructose / Q000032:analysis*; D020576:Gluconobacter / Q000201:enzymology*; D006046:Gold / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D005632:Fructose; D006046:Gold; D002237:Carbohydrate Dehydrogenases; C028761:D-fructose 5-dehydrogenase