title: Purification of a lectin from Arisaema erubescens (Wall.) Schott and its pro-inflammatory effects.
abstract: The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E(2 )(PGE(2)) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 μg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research.
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, 210046, China.
authors.forename: Xian Qiong
authors.initials: XQ
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Hong Li
authors.initials: HL
authors.lastname: Yu
authors.forename: Teng Fei
authors.initials: TF
authors.lastname: Zhao
authors.forename: Yao Zong
authors.initials: YZ
authors.lastname: Pan
authors.forename: Run Jun
authors.initials: RJ
authors.lastname: Shi
mesh_terms: D000818:Animals; D031057:Arisaema / Q000033:anatomy & histology / Q000737:chemistry*; D001212:Asialoglycoproteins / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D015232:Dinoprostone / Q000276:immunology; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004912:Erythrocytes / Q000187:drug effects; D060748:Fetuins / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008663:Metacarpus / Q000473:pathology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000276:immunology; D037121:Plant Lectins / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D001212:Asialoglycoproteins; D060748:Fetuins; D037121:Plant Lectins; D014409:Tumor Necrosis Factor-alpha; C017029:asialofetuin; D009569:Nitric Oxide; D003189:p-Methoxy-N-methylphenethylamine; D015232:Dinoprostone
references.citation: J Immunol. 1990 Feb 15;144(4):1425-31
references.pmid: 2303713
references.citation: Int Arch Allergy Immunol. 1997 Jan;112(1):27-35
references.pmid: 8980461
references.citation: Mem Inst Oswaldo Cruz. 1986 Jul-Sep;81(3):343-5
references.pmid: 3553847
references.citation: J Pathol. 1971 May;104(1):15-29
references.pmid: 4398139
references.citation: Science. 1978 Oct 20;202(4365):320-1
references.pmid: 694537
references.citation: J Clin Invest. 1990 Nov;86(5):1556-64
references.pmid: 2173722
references.citation: Eur J Biochem. 1986 Sep 1;159(2):239-45
references.pmid: 3758061
references.citation: J Biochem Mol Biol. 2005 Sep 30;38(5):526-32
references.pmid: 16202230
references.citation: Inflamm Res. 2010 Apr;59(4):245-54
references.pmid: 19757085
references.citation: Phytochemistry. 1995 Sep;40(2):449-55
references.pmid: 7546557
references.citation: Protein Pept Lett. 2004 Apr;11(2):195-200
references.pmid: 15078209
references.citation: Zhongguo Zhong Yao Za Zhi. 2010 May;35(9):1152-5
references.pmid: 20707072
references.citation: J Asian Nat Prod Res. 2010 Feb;12(2):157-61
references.pmid: 20390760
references.citation: Int J Biochem Cell Biol. 2003 Dec;35(12):1674-81
references.pmid: 12962707
references.citation: Agents Actions. 1988 Jul;24(3-4):377-80
references.pmid: 3051931
references.citation: Int J Biochem Cell Biol. 2005 Sep;37(9):1805-14
references.pmid: 15914076
references.citation: J Pharm Pharmacol. 2005 Mar;57(3):375-81
references.pmid: 15807994
references.citation: Tissue Antigens. 2004 Jul;64(1):1-12
references.pmid: 15191517
references.citation: Antiviral Res. 1992 Jun;18(2):191-207
references.pmid: 1329650
references.citation: Naunyn Schmiedebergs Arch Pharmacol. 2006 Oct;374(1):1-10
references.pmid: 16957941
references.citation: Biochem Biophys Res Commun. 2000 Sep 7;275(3):810-6
references.pmid: 10973803
references.citation: J Pharm Pharmacol. 1979 Sep;31(9):648
references.pmid: 41077
references.citation: Biosci Rep. 2005 Oct-Dec;25(5-6):345-62
references.pmid: 16307381
references.citation: Crit Rev Immunol. 2004;24(3):193-203
references.pmid: 15482254
references.citation: Agents Actions. 1993 Jan;38(1-2):48-54
references.pmid: 8480538
references.citation: Agents Actions. 1990 Jun;30(3-4):344-9
references.pmid: 2201176
references.citation: Peptides. 2009 Oct;30(10):1805-15
references.pmid: 19577602
references.citation: Cell Immunol. 1999 May 25;194(1):98-102
references.pmid: 10357885
references.citation: Immunology. 1991 Aug;73(4):472-7
references.pmid: 1916898
references.citation: Agents Actions. 1985 Oct;17(1):97-103
references.pmid: 4083185
references.citation: Cell. 2001 Feb 23;104(4):487-501
references.pmid: 11239407