title: Silica-modified oligonucleotide-gold nanoparticle conjugate enables closed-tube colorimetric polymerase chain reaction.
abstract: A facile silica coating significantly enhances the thermal stability and polymerase chain reaction (PCR) compatibility of oligonucleotide-gold nanoparticle conjugates, thus enabling colorimetric detection of PCR results in a closed-tube format. This method is specific, sensitive, and generally applicable. Its simplicity, visual readout, and carryover contamination-free features hold promise for point-of-care or on-site DNA testing.
authors.affiliation: Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
authors.forename: Jacky K F
authors.initials: JK
authors.lastname: Wong
authors.forename: Shea Ping
authors.initials: SP
authors.lastname: Yip
authors.forename: Thomas M H
authors.initials: TM
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D006046:Gold; D053768:Metal Nanoparticles; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012822:Silicon Dioxide
chemical_list: D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D006046:Gold; D012822:Silicon Dioxide; D004247:DNA