title: [Effect of ERK1/2 signaling pathway on astragaloside IV protects H9c2 cells against H2O2-induced oxidative injury].
abstract: OBJECTIVE
  To investigate whether Astragaloside IV(AST) protects H9c2 cells against H2O2-induced oxidative injury partly through ERK1/2 signaling pathway.
  
  
  METHODS
  H9c2 cells oxidative injury was induced by 200 tmol/L H2O2 for 6 hours to establish the H2O2-induced injury model of H9c2 cells. The viability of H9c2 cells was detected using MTf method. Activity of lactate dehydrogenase(LDH), total-superoxide dismutase (T-SOD), manganese-superoxide dismutase (Mn-SOD) and content of MDA (malondialdehyde) in the culture medium were detected using colorimetric method. Western blot was performed to exam expression of p-ERK1/2 and ERK1/2 in H9c2 cells respectively.
  
  
  RESULTS
  Under 200 micromol/L H2O2 treatment for 6 hours, the vaibility of H9c2 cells was suitable for the following study. Compared with H2O2 group, the cell viability was increased significantly in AST10 + H2O2 and AST2O + H2O2 groups (P < 0.01). The activity of LDH in the culture medium was decreased significantly (P < 0.01). The activity of T-SOD and Mn-SOD was increased significantly (P < 0.01), the content of MDA was decreased significantly (P < 0.01). Treated with 10 mg/L or 20 mg/L of AST, expression of p-ERK1/2 in H9c2 cells injured from H2O2 was increased significantly (P < 0.01), when PD98059 (inhibitor of ERK1/2) was added, the effects of AST were cancelled.
  
  
  CONCLUSIONS
  AST protects H9c2 cells against H2O2-induced oxidative injury partly through ERK1/2 signaling pathway.
authors.affiliation: Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Peng
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yan-na
authors.initials: YN
authors.lastname: Wu
authors.forename: Jun-qiu
authors.initials: JQ
authors.lastname: Song
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000633:toxicity*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D032386:Myoblasts, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D006861:Hydrogen Peroxide