title: Tandem multimer expression and preparation of hypoglycemic peptide MC6 from Momordica charantia in Escherichia coli.
abstract: Tandem repeat multimers of Momordica charantia (MC) peptide MC6 were designed and the recombinant plasmid containing 10 copies of MC6 gene was constructed to improve the expression level of MC6 in Escherichia coli. Under the selected conditions of cultivation and induction, the expression level of recombinant TrxA-MC6(10) protein was above 25% of total bacteria protein. This fusion protein was purified and cleaved with HCl (13%, w/v). Either the un-cleaved or cleaved recombinant proteins was analyzed pharmacological activity by alloxan-induced diabetic mice and only the cleaved products of the recombinant protein showed significant hypoglycemic effects. The study provides a convenient and economical method for the large-scale production of anti-diabetic medicines for pharmaceutical applications.
authors.affiliation: Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Wang
authors.forename: Huai-Lei
authors.initials: HL
authors.lastname: Song
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Wang
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Bi-Lian
authors.initials: BL
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhi-Bi
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D015870:Gene Expression; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D029777:Momordica charantia / Q000737:chemistry*; D010455:Peptides / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial
chemical_list: D001786:Blood Glucose; D029968:Escherichia coli Proteins; D007004:Hypoglycemic Agents; D010455:Peptides; D011993:Recombinant Fusion Proteins; C517609:TrxA protein, E coli; D013879:Thioredoxins; D000496:Alloxan; D006851:Hydrochloric Acid