title: Isolation and proteomic analysis of platelets by SELDI-TOF MS.
abstract: Many growth factors, leukotrines, and biological ligands are not circulating free in plasma or serum, except in the case of late or disseminated disease. During early tumor growth and angiogenesis, platelets actively and selectively sequester regulators of angiogenesis and, as such, the platelet protein content can be used as a marker of early tumor growth or angiogenesis. With the recent increase in the clinical use of biologic modifiers in cancer and chronic disease therapy, the search for markers of early disease, therapeutic response, and/or recurrence has suggested that analysis of platelet proteins may be more relevant and accurate. We provide a guideline for the proteomic analysis of platelet proteome, placing specific emphasis on angiogenesis regulators, even though other platelet proteins may serve as markers of disease in the future. The analysis of serum/plasma has been fraught with difficulties because of the extraordinarily large number of proteins and because some of the proteins are contained in extraordinarily large amounts, masking the less abundant proteins. Thus, platelets may provide a much more biologically relevant analyte for biomarker discovery.
authors.affiliation: Vascular Biology Program, Children's Hospital Boston, Boston, MA, USA. sdowningIII@verison.net
authors.forename: Sean R
authors.initials: SR
authors.lastname: Downing
authors.forename: Giannoula L
authors.initials: GL
authors.lastname: Klement
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism*; D001800:Blood Specimen Collection; D002469:Cell Separation / Q000379:methods*; D005591:Chemical Fractionation; D006801:Humans; D051379:Mice; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001798:Blood Proteins
references.citation: Br J Cancer. 1999 Feb;79(3-4):451-5
references.pmid: 10027312
references.citation: Cancer Res. 2006 Oct 15;66(20):9804-8
references.pmid: 17047040
references.citation: Oncologist. 1998;3(2):II
references.pmid: 10388096
references.citation: Anticancer Res. 2000 Jan-Feb;20(1B):569-72
references.pmid: 10769727
references.citation: N Engl J Med. 2007 Dec 13;357(24):2482-94
references.pmid: 18077812
references.citation: APMIS. 2002 Sep;110(9):646-50
references.pmid: 12529018
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17041-6
references.pmid: 16286641
references.citation: World J Gastroenterol. 2006 Nov 7;12(41):6689-92
references.pmid: 17075985
references.citation: Dis Mon. 1989 Nov;35(11):721-68
references.pmid: 2680358
references.citation: Clin Chem. 2006 Sep;52(9):1669-74
references.pmid: 16840585
references.citation: Clin Cancer Res. 1997 Dec;3(12 Pt 1):2451-8
references.pmid: 9815646
references.citation: Cancer Epidemiol Biomarkers Prev. 2006 Jun;15(6):1115-9
references.pmid: 16775168
references.citation: Blood. 1985 Jun;65(6):1299-311
references.pmid: 3888311
references.citation: BMC Cancer. 2006 May 24;6:136
references.pmid: 16723013
references.citation: Clin Cancer Res. 1997 Dec;3(12 Pt 1):2187-90
references.pmid: 9815613
references.citation: Mol Cell Proteomics. 2002 Nov;1(11):845-67
references.pmid: 12488461
references.citation: Lung Cancer. 2006 Oct;54(1):87-94
references.pmid: 16876904
references.citation: Circ Res. 2006 Feb 3;98(2):e2-10
references.pmid: 16373597
references.citation: J Natl Cancer Inst. 2006 Sep 6;98(17):1193-203
references.pmid: 16954472
references.citation: Blood. 2008 Feb 1;111(3):1201-7
references.pmid: 17914028
references.citation: J Huazhong Univ Sci Technolog Med Sci. 2006;26(3):311-3
references.pmid: 16961278
references.citation: Vnitr Lek. 2006 Mar;52 Suppl 1:135-8
references.pmid: 16637463
references.citation: Invest New Drugs. 1997;15(1):29-37
references.pmid: 9195287
references.citation: Clin Cancer Res. 2006 Oct 1;12(19):5705-12
references.pmid: 17020974
references.citation: Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7481-6
references.pmid: 11404464
references.citation: Int J Cancer. 2000 Jan 1;85(1):40-5
references.pmid: 10585580
references.citation: N Engl J Med. 1971 Nov 18;285(21):1182-6
references.pmid: 4938153
references.citation: Onkologie. 2006 Nov;29(11):498-9
references.pmid: 17068383
references.citation: Gastroenterology. 2006 May;130(6):1917-9
references.pmid: 16697755
references.citation: Am J Pathol. 1997 Mar;150(3):815-21
references.pmid: 9060819
references.citation: Blood. 2009 Mar 19;113(12):2835-42
references.pmid: 19036702
references.citation: Nat Clin Pract Gastroenterol Hepatol. 2006 Nov;3(11):594-5
references.pmid: 17068491
references.citation: Am J Physiol. 1986 Mar;250(3 Pt 2):H366-71
references.pmid: 3006516