title: X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85β subunit.
abstract: Src-homology 3 (SH3) domains are involved in extensive protein-protein interactions and constitute key elements of intracellular signal transduction. Three-dimensional structures have been reported for SH3 domains of various proteins, including the 85 kDa regulatory subunit (p85) of phosphoinositide 3-kinase. However, all of the latter structures are of p85 isoform α and no crystal structure of the SH3 domain of the equally important isoform β has been reported to date. In this structural communication, the recombinant production, crystallization and X-ray structure determination at 2.0 Å resolution of the SH3 domain of human p85β is described. The structure reveals a compact β-barrel fold very similar to that of p85α. However, binding studies with two classes of proline-rich ligand peptides demonstrate that the ligand-binding specificity differs slightly between the SH3 domains of human p85β and p85α, despite their high structural similarity.
authors.affiliation: Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.forename: Yibei
authors.initials: Y
authors.lastname: Xiao
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ponnusamy
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Tan
authors.forename: Jian
authors.initials: J
authors.lastname: Lei
authors.forename: Rolf
authors.initials: R
authors.lastname: Hilgenfeld
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D021122:Protein Subunits / Q000737:chemistry; D016415:Sequence Alignment; D018909:src Homology Domains
chemical_list: D021122:Protein Subunits; D019869:Phosphatidylinositol 3-Kinases
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