title: Extension of bladder-based organ regeneration platform for tissue engineering of esophagus.
abstract: Recent successes in regenerative medicine and tissue engineering of bladder and bladder-like neo-organs have leveraged regenerative constructs composed of a biodegradable scaffold seeded with a population of smooth muscle cells. We have shown that such smooth muscle cells are isolatable from adipose and other sources alternate to the primary organ. We hypothesize that this regenerative platform is not limited to regeneration of bladder and bladder-like neo-organs, but rather represents a foundational technology platform broadly applicable for regeneration of laminarly organized hollow organs. Using esophagus as an illustrative example in support of this hypothesis, we demonstrate that patch constructs composed of adipose-derived smooth muscle cells seeded on a biodegradable matrix catalyze complete regeneration of the esophageal wall in a rodent model of esophageal injury. By implication, such regenerative constructs may potentially be used to mediate the regeneration of any laminarly organized tubular organ.
authors.affiliation: Tengion Inc., 3929 Westpoint Blvd., Suite G, Winston-Salem, NC 27103, USA. joydeep.basu@tengion.com
authors.forename: Joydeep
authors.initials: J
authors.lastname: Basu
authors.forename: Kim L
authors.initials: KL
authors.lastname: Mihalko
authors.forename: Richard
authors.initials: R
authors.lastname: Payne
authors.forename: Elias
authors.initials: E
authors.lastname: Rivera
authors.forename: Toyin
authors.initials: T
authors.lastname: Knight
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Genheimer
authors.forename: Kelly I
authors.initials: KI
authors.lastname: Guthrie
authors.forename: Namrata
authors.initials: N
authors.lastname: Sangha
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Jayo
authors.forename: Deepak
authors.initials: D
authors.lastname: Jain
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Bertram
authors.forename: John W
authors.initials: JW
authors.lastname: Ludlow
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D004947:Esophagus / Q000502:physiology*; D005260:Female; D032389:Myocytes, Smooth Muscle / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds; D001743:Urinary Bladder / Q000502:physiology*