title: Regulation of reactionary dentin formation by odontoblasts in response to polymicrobial invasion of dentin matrix.
abstract: Odontoblast synthesis of dentin proceeds through discrete but overlapping phases characterized by formation of a patterned organic matrix followed by remodelling and active mineralization. Microbial invasion of dentin in caries triggers an adaptive response by odontoblasts, culminating in formation of a structurally altered reactionary dentin, marked by biochemical and architectonic modifications including diminished tubularity. Scanning electron microscopy of the collagen framework in reactionary dentin revealed a radically modified yet highly organized meshwork as indicated by fractal and lacunarity analyses. Immuno-gold labelling demonstrated increased density and regular spatial distribution of dentin sialoprotein (DSP) in reactionary dentin. DSP contributes putative hydroxyapatite nucleation sites on the collagen scaffold. To further dissect the formation of this altered dentin matrix, the associated enzymatic machinery was investigated. Analysis of extracted dentin matrix indicated increased activity of matrix metalloproteinase-2 (MMP-2) in the reactionary zone referenced to physiologic dentin. Likewise, gene expression analysis of micro-dissected odontoblast layer revealed up-regulation of MMP-2. Parallel up-regulation of tissue inhibitor of metalloproteinase-2 (TIMP-2) and membrane type 1- matrix metalloproteinase (MT1-MMP) was observed in response to caries. Next, modulation of odontoblastic dentinogenic enzyme repertoire was addressed. In the odontoblast layer expression of Toll-like receptors was markedly altered in response to bacterial invasion. In carious teeth TLR-2 and the gene encoding the corresponding adaptor protein MyD88 were down-regulated whereas genes encoding TLR-4 and adaptor proteins TRAM and Mal/TIRAP were up-regulated. TLR-4 signalling mediated by binding of bacterial products has been linked to up-regulation of MMP-2. Further, increased expression of genes encoding components of the TGF-β signalling pathway, namely SMAD-2 and SMAD-4, may explain the increased synthesis of collagen by odontoblasts in caries. These findings indicate a radical adaptive response of odontoblasts to microbial invasion of dentin with resultant synthesis of modified mineralized matrix.
authors.affiliation: Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Sydney, New South Wales, Australia. ncha1431@uni.sydney.edu.au
authors.forename: Nattida
authors.initials: N
authors.lastname: Charadram
authors.forename: Ramin M
authors.initials: RM
authors.lastname: Farahani
authors.forename: Derek
authors.initials: D
authors.lastname: Harty
authors.forename: Catherine
authors.initials: C
authors.lastname: Rathsam
authors.forename: Michael V
authors.initials: MV
authors.lastname: Swain
authors.forename: Neil
authors.initials: N
authors.lastname: Hunter
mesh_terms: D000328:Adult; D001419:Bacteria / Q000378:metabolism / Q000472:pathogenicity; D003731:Dental Caries / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D003804:Dentin / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology* / Q000648:ultrastructure*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005260:Female; D018093:Gelatinases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism* / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D018093:Gelatinases; D053511:Matrix Metalloproteinase 14; D020778:Matrix Metalloproteinase 2; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
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