title: Spaceflight alters the gene expression profile of cervical cancer cells.
abstract: Our previous study revealed that spaceflight induced biological changes in human cervical carcinoma Caski cells. Here, we report that 48A9 cells, which were subcloned from Caski cells, experienced significant growth suppression and exhibited low tumorigenic ability after spaceflight. To further understand the potential mechanism at the transcriptional level, we compared gene expression between 48A9 cells and ground control Caski cells with suppression subtractive hybridization (SSH) and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis (IPA) program. We found 5 genes, SUB1, SGEF, MALAT-1, MYL6, and MT-CO2, to be up-regulated and identified 3 new cDNAs, termed B4, B5, and C4, in 48A9 cells. In addition, we also identified the two most significant gene networks to indicate the function of these genes using the IPA program. To our knowledge, our results show for the first time that spaceflight can reduce the growth of tumor cells, and we also provide a new model for oncogenesis study.
authors.affiliation: Xiangya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Tong
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Zhou
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Ping-Li
authors.initials: PL
authors.lastname: Xie
authors.forename: Jin-Yue
authors.initials: JY
authors.lastname: Hu
authors.forename: Guan-Cheng
authors.initials: GC
authors.lastname: Li
mesh_terms: D015152:Blotting, Northern / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D053263:Gene Regulatory Networks; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D013026:Space Flight; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
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