title: Characterization of a novel Xenopus tropicalis cell line as a model for in vitro studies.
abstract: Cell lines are useful tools to facilitate in vitro studies of many biological and molecular processes. We describe a new permanent fibroblast-type cell line obtained from disaggregated Xenopus tropicalis limb bud. The cell line population doubling time was ~24 h. Its karyotype was genetically stable with a chromosome number of 2n = 21 and a chromosome 10 trisomy. These cells could be readily transfected and expressed transgenes faithfully. We obtained stable transformants using transposon-based gene transfer technology. These cells responded to thyroid hormone and thus can provide a complementary research tool to study thyroid hormone signaling events. In conclusion, this cell line baptized "Speedy" should prove useful to couple in vitro and in vivo biological studies in the X. tropicalis frog model.
authors.affiliation: Institute of Systems and Synthetic Biology, Genopole, CNRS, Université d'Evry Val d'Essonne, Genavenir 3-Genopole campus 3, 1 rue Pierre Fontaine, Evry, France.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Sinzelle
authors.forename: Raphaël
authors.initials: R
authors.lastname: Thuret
authors.forename: Ho-Yon
authors.initials: HY
authors.lastname: Hwang
authors.forename: Bérénice
authors.initials: B
authors.lastname: Herszberg
authors.forename: Elodie
authors.initials: E
authors.lastname: Paillard
authors.forename: Odile J
authors.initials: OJ
authors.lastname: Bronchain
authors.forename: Derek L
authors.initials: DL
authors.lastname: Stemple
authors.forename: Sophie
authors.initials: S
authors.lastname: Dhorne-Pollet
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pollet
mesh_terms: D000818:Animals; D002460:Cell Line; D004251:DNA Transposable Elements; D023061:Gene Order; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D059785:Karyotype; D061251:Primary Cell Culture; D015398:Signal Transduction / Q000187:drug effects; D013963:Thyroid Hormones / Q000494:pharmacology; D014162:Transfection; D019076:Transgenes; D014981:Xenopus / Q000235:genetics* / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D013963:Thyroid Hormones
references.citation: J Biol Chem. 1992 Jan 15;267(2):739-45
references.pmid: 1730665
references.citation: Virology. 1989 Sep;172(1):156-69
references.pmid: 2549707
references.citation: Nucleic Acids Res. 2007;35(12):e87
references.pmid: 17576687
references.citation: Cell. 1997 Nov 14;91(4):501-10
references.pmid: 9390559
references.citation: Experientia. 1992 Jan 15;48(1):87-91
references.pmid: 1737584
references.citation: Methods. 2009 Nov;49(3):287-97
references.pmid: 19615447
references.citation: Mol Ther. 2010 Jun;18(6):1200-9
references.pmid: 20372108
references.citation: Nat Genet. 2009 Jun;41(6):753-61
references.pmid: 19412179
references.citation: Methods Cell Biol. 1991;36:635-54
references.pmid: 1811155
references.citation: Dev Dyn. 2009 Jul;238(7):1727-43
references.pmid: 19517568
references.citation: Virology. 1995 Aug 20;211(2):397-407
references.pmid: 7645244
references.citation: In Vitro. 1981 Apr;17(4):267-74
references.pmid: 7239540
references.citation: Experientia. 1973 Apr 15;29(4):466-7
references.pmid: 4708349
references.citation: In Vitro. 1977 Sep;13(9):557-63
references.pmid: 562840
references.citation: J Biol Chem. 2000 Apr 7;275(14):10484-91
references.pmid: 10744739
references.citation: Exp Cell Res. 1974 Aug;87(2):433-6
references.pmid: 4472348
references.citation: J Mol Biol. 2002 May 17;318(5):1221-35
references.pmid: 12083513
references.citation: In Vitro Cell Dev Biol. 1990 Dec;26(12):1128-34
references.pmid: 1706696
references.citation: Nat Protoc. 2006;1(3):1559-82
references.pmid: 17406449
references.citation: Mob DNA. 2010 Dec 07;1(1):25
references.pmid: 21138556
references.citation: Transgenic Res. 2006 Dec;15(6):751-60
references.pmid: 16957880
references.citation: Dev Dyn. 2007 Oct;236(10):2808-17
references.pmid: 17879322
references.citation: Mol Cell Endocrinol. 1992 Sep;87(1-3):105-13
references.pmid: 1446782
references.citation: Cell. 2005 Aug 12;122(3):473-83
references.pmid: 16096065
references.citation: Prog Clin Biol Res. 1986;226:55-66
references.pmid: 3809206
references.citation: Dev Dyn. 2009 Jun;238(6):1398-46
references.pmid: 19441086
references.citation: Cytogenet Genome Res. 2007;116(1-2):110-2
references.pmid: 17268187
references.citation: Dev Dyn. 2006 Mar;235(3):754-8
references.pmid: 16397894
references.citation: Environ Toxicol Chem. 2005 Mar;24(3):653-64
references.pmid: 15779766
references.citation: BMC Biol. 2008 Sep 15;6:40
references.pmid: 18793381