title: Prognostic significance of CD83 positive tumor-infiltrating dendritic cells and expression of TGF-beta 1 in human gastric cancer.
abstract: OBJECTIVE
  In this study we analyzed the significance of CD1a and CD83 positive tumor infiltrating dendritic cells (TIDCs) and the expression of TGF-β1 in gastric cancer tissue, and their relationship with disease progression and prognosis of patients.
  
  
  METHODS
  The immunohistochemical expression of CD1a, CD83 and TGF-β1, was evaluated in 55 patients with gastric cancer and followed-up for five years.
  
  
  RESULTS
  We found tumor infiltration with CD1a and CD83 positive DCs in all 55 cases and cytoplasmic TGF-β1 immunoreactivity in tumor cells in 76.4% of cases. TGF-β1 expression correlated to low CD83 positive DCs in 100% of the samples (χ2=7.66; p=0.022). Low CD83 positive DCs in tumor border (χ2=15.38; p<0.001) was also observed in 100% of tumors with TGF-β1 expression. The number of CD1a and CD83 positive TIDCs in the tumor border was inversely correlated with positive lymph node metastases (χ2=6.64; p=0.036 and χ2=6.44; p<0.04, respectively). Patients with a low number of tumor infiltrating CD83 positive DCs had shorter survival rates (p=0.022) and patients with TGF-β1 expression had a worse prognosis after surgical therapy (p=0.017).
  
  
  CONCLUSIONS
  Our results suggest that tumor infiltration with DCs may be of great importance in initiating the primary anti-tumor immune response. In patients with resectable gastric cancer, the grade of TIDCs and TGF-β1 expression could be a useful predictor of prognosis.
authors.affiliation: Department of General and Clinical Pathology, Trakia University, Bulgaria. operation@abv.bg
authors.forename: Julian
authors.initials: J
authors.lastname: Ananiev
authors.forename: Maya Vladova
authors.initials: MV
authors.lastname: Gulubova
authors.forename: Irena Manolova
authors.initials: IM
authors.lastname: Manolova
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D018949:Antigens, CD1 / Q000032:analysis*; D016009:Chi-Square Distribution; D003713:Dendritic Cells / Q000737:chemistry*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis; D009367:Neoplasm Staging; D011379:Prognosis; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000601:surgery; D015996:Survival Rate; D016212:Transforming Growth Factor beta / Q000032:analysis*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D016212:Transforming Growth Factor beta; D000097802:CD83 Antigen