title: [STAT5 phosphorylation levels of erythropoietin and thrombopoietin receptors in CD34(+)CD59(-) and CD34(+)CD59(+) bone marrow cells of patients with paroxysmal nocturnal hemoglobinuria].
abstract: OBJECTIVE
  To study the STAT5 phosphorylation levels of erythropoietin receptor (EPOR) and thrombopoietin receptor (TPOR) in CD34(+)CD59(-) and CD34(+)CD59(+) bone marrow cells of the patients with paroxysmal nocturnal hemoglobinuria (PNH).
  
  
  METHODS
  The bone marrow mononuclear cells (BMMNC) were extracted from 23 PNH patients treated at our department from April 2010 to February 2011 and 11 normal controls. The mean fluorescence intensity (MFI) of phosphorylated STAT5 (P-STAT5) in CD34(+)CD59(+) cells and CD34(+)CD59(-) cells with or without the stimulation of 10 U/ml EPO and 50 U/ml TPO were examined by flow cytometry.
  
  
  RESULTS
  (1) Without stimulation, the P-STAT5 MFI in CD34(+)CD59(-) cells of PNH patients was significantly lower than that of CD34(+)CD59(+) cells (31 ± 15 vs 74 ± 47, P < 0.01). And it was 59 ± 23 in normal control CD34(+)CD59(+) cells (P < 0.05). No statistic difference existed between the CD34(+)CD59(+) cells of PNH patients and the normal control CD34(+)CD59(+) cells. (2) Under the stimulations of EPO and TPO, the P-STAT5 MFI was significantly lower in CD34(+)CD59(-) cells of PNH patients than that of CD34(+)CD59(+) cells (49 ± 24 and 51 ± 41 vs 120 ± 82 and 124 ± 87, both P < 0.01). For the normal control CD34(+)CD59(+) cells, they were 79 ± 47 and 98 ± 53 respectively (P < 0.05). No statistic difference existed between the CD34(+)CD59(+) cells of PNH patients and the normal control CD34(+)CD59(+) cells. P-STAT5 MFI was elevated after the stimulations of EPO and TPO. The increments of CD34(+)CD59(+) cells in PNH patients were significantly higher than those of CD34(+)CD59(-) cells (49 ± 11 and 54 ± 43 vs 17 ± 4 and 16 ± 6, both P < 0.01).
  
  
  CONCLUSIONS
  Under the in vitro stimulations of EPO and TPO, the STAT5 phosphorylation levels of EPO and TPO receptors in normally cloned hematopoietic stem cells in PNH patients are obviously superior to those in abnormally cloned counterparts.
authors.affiliation: Department of Hematology & Oncology, Tianjin Medical University General Hospital, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Er-bao
authors.initials: EB
authors.lastname: Ruan
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Hua-quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Li-min
authors.initials: LM
authors.lastname: Xing
authors.forename: Guo-jin
authors.initials: GJ
authors.lastname: Wang
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Zong-hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000378:metabolism*; D018957:CD59 Antigens; D016022:Case-Control Studies; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006457:Hemoglobinuria, Paroxysmal / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010085:Oxidative Phosphorylation; D017467:Receptors, Erythropoietin / Q000378:metabolism*; D053628:Receptors, Thrombopoietin / Q000378:metabolism*; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D018957:CD59 Antigens; D017467:Receptors, Erythropoietin; D053628:Receptors, Thrombopoietin; D050799:STAT5 Transcription Factor