title: Expanding the linear dynamic range for multiple reaction monitoring in quantitative liquid chromatography-tandem mass spectrometry utilizing natural isotopologue transitions.
abstract: We describe a method for expanding the linear dynamic range for multiple reaction monitoring (MRM) in quantitative liquid chromatography/tandem mass spectrometry (LC-MS/MS) using additional transitions for isotopologues. In addition to the regular transition for the highest possible sensitivity, a transition corresponding to the less abundant isotopologue ions was utilized. This decreases saturation at the ion detector; the sensitivity reduction increases the upper dynamic limit. We demonstrated this for a rat plasma assay for a candidate flavor compound; the linear dynamic range increased by an order of magnitude from 3 to 6,000 ng/mL with the regular MRM alone to 3-60,000 ng/mL using additionally the isotopologue transition.
authors.affiliation: Senomyx Inc., 4767 Nexus Centre Drive, San Diego, CA 92121, United States. Hanghui.Liu@senomyx.com
authors.forename: Hanghui
authors.initials: H
authors.lastname: Liu
authors.forename: Lily
authors.initials: L
authors.lastname: Lam
authors.forename: Purnendu K
authors.initials: PK
authors.lastname: Dasgupta
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005421:Flavoring Agents / Q000493:pharmacokinetics*; D016014:Linear Models; D051381:Rats; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005421:Flavoring Agents