title: Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves.
abstract: Junctional adhesion molecule-C (JAM-C) is an adhesion molecule expressed at junctions between adjacent endothelial and epithelial cells and implicated in multiple inflammatory and vascular responses. In addition, we recently reported on the expression of JAM-C in Schwann cells (SCs) and its importance for the integrity and function of peripheral nerves. To investigate the role of JAM-C in neuronal functions further, mice with a specific deletion of JAM-C in SCs (JAM-C SC KO) were generated. Compared to wild-type (WT) controls, JAM-C SC KO mice showed electrophysiological defects, muscular weakness, and hypersensitivity to mechanical stimuli. In addressing the underlying cause of these defects, nerves from JAM-C SC KO mice were found to have morphological defects in the paranodal region, exhibiting increased nodal length as compared to WTs. The study also reports on previously undetected expressions of JAM-C, namely on perineural cells, and in line with nociception defects of the JAM-C SC KO animals, on finely myelinated sensory nerve fibers. Collectively, the generation and characterization of JAM-C SC KO mice has provided unequivocal evidence for the involvement of SC JAM-C in the fine organization of peripheral nerves and in modulating multiple neuronal responses.
authors.affiliation: William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, London EC1M6BQ, UK.
authors.forename: Bartomeu
authors.initials: B
authors.lastname: Colom
authors.forename: Yannick
authors.initials: Y
authors.lastname: Poitelon
authors.forename: Wenlong
authors.initials: W
authors.lastname: Huang
authors.forename: Abigail
authors.initials: A
authors.lastname: Woodfin
authors.forename: Sharon
authors.initials: S
authors.lastname: Averill
authors.forename: Ubaldo
authors.initials: U
authors.lastname: Del Carro
authors.forename: Desirée
authors.initials: D
authors.lastname: Zambroni
authors.forename: Susan D
authors.initials: SD
authors.lastname: Brain
authors.forename: Mauro
authors.initials: M
authors.lastname: Perretti
authors.forename: Amrita
authors.initials: A
authors.lastname: Ahluwalia
authors.forename: John V
authors.initials: JV
authors.lastname: Priestley
authors.forename: Triantafyllos
authors.initials: T
authors.lastname: Chavakis
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof
authors.forename: M Laura
authors.initials: ML
authors.lastname: Feltri
authors.forename: Sussan
authors.initials: S
authors.lastname: Nourshargh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D042783:Endothelial Cells / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D007136:Immunoglobulins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000293:injuries / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D010525:Peripheral Nerves / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D012018:Reflex / Q000502:physiology; D012583:Schwann Cells / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D011984:Sensory Receptor Cells / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C486723:Jam3 protein, mouse; D015740:Calcitonin Gene-Related Peptide
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