title: Human miR-31 targets radixin and inhibits migration and invasion of             glioma cells.
abstract: MicroRNAs (miRNAs) are a novel group of short RNAs, about 20‑22 nucleotide             in length, that regulate gene expression in a post-transcriptional manner by affecting             the stability or translation of mRNAs and play important roles in many biological             processes. Many microRNAs have been implicated in glioblastoma. miR-31 is dysregulated             in several types of cancer including colon, breast, prostate, gastric and lung             cancers. However, the expression and role of miR-31 in glioblastoma are still             unclear. In this study, we performed real-time reverse transcriptase polymerase             chain reaction (RT-PCR) assays on 10 glioblastoma and 7 normal brain tissues.             We found that miR-31 is down-regulated in glioblastoma compared with normal brain             tissues. Ectopic expression of miR-31 inhibited migration and invasion ability             of U251 glioma cells. Expression profiling analysis revealed that miR-31 affected             the cell migration and motility process by regulating migration and invasion related             genes. Finally, we demonstrated that miR-31 targeted radixin predominantly via             inhibition of protein translation instead of degradation of mRNA.
authors.affiliation: Division of Systems Biology, Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Dasong
authors.initials: D
authors.lastname: Hua
authors.forename: Dong
authors.initials: D
authors.lastname: Ding
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Weiyi
authors.initials: W
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.forename: Gregory
authors.initials: G
authors.lastname: Foltz
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003598:Cytoskeletal Proteins; C534612:MIRN31 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; C071776:radixin