title: Glucagon-like peptide 1 receptor plays a critical role in geniposide-regulated insulin secretion in INS-1 cells.
abstract: OBJECTIVE
  To explore the role of the glucagon-like peptide 1 receptor (GLP-1R) in geniposide regulated insulin secretion in rat INS-1 insulinoma cells.
  
  
  METHODS
  Rat INS-1 insulinoma cells were cultured. The content of insulin in the culture medium was measured with ELISA assay. GLP-1R gene in INS-1 cells was knocked down with shRNA interference. The level of GLP-1R protein in INS-1 cells was measured with Western blotting.
  
  
  RESULTS
  Geniposide (0.01-100 μmol/L) increased insulin secretion from INS-1 cells in a concentration-dependent manner. Geniposide (10 μmol/L) enhanced acute insulin secretion in response to both the low (5.5 mmol/L) and moderately high levels (11 mmol/L) of glucose. Blockade of GLP-1R with the GLP-1R antagonist exendin (9-39) (200 nmol/L) or knock-down of GLP-1R with shRNA interference in INS-1 cells decreased the effect of geniposide (10 μmol/L) on insulin secretion stimulated by glucose (5.5 mmol/L).
  
  
  CONCLUSIONS
  Geniposide increases insulin secretion through glucagon-like peptide 1 receptors in rat INS-1 insulinoma cells.
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing, China.
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Guo
authors.forename: Zhi-ning
authors.initials: ZN
authors.lastname: Xia
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Yin
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism; D032062:Gardenia / Q000737:chemistry*; D052216:Glucagon-Like Peptide 1 / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D039823:Iridoids / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats
chemical_list: D007328:Insulin; D039823:Iridoids; D010936:Plant Extracts; D034741:RNA, Small Interfering; C007835:geniposide; D052216:Glucagon-Like Peptide 1
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