title: Visualisation of morphological interaction of diamond and silver nanoparticles with Salmonella Enteritidis and Listeria monocytogenes.
abstract: Currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. The objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with Salmonella Enteritidis (G-) or Listeria monocytogenes (G+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. Diamond nanoparticles (nano-D) were produced by the detonation method. Hydrocolloids of silver nanoparticles (nano-Ag) were produced by electric non-explosive patented method. Hydrocolloids of nanoparticles (200 microl) were added to bacteria suspension (200 microl) in the following order: nano-D + Salmonella E.; nano-D + Listeria monocytogenes; nano-Ag + Salmonella E; nano-Ag + Listeria monocytogenes. Samples were inspected by transmission electron microscopy. Visualisation of nanoparticles and bacteria interaction showed harmful effects of both nanoparticles on bacteria morphology. The most spectacular effect of nano-D were strong links between nano-D packages and the flagella of Salmonella E. Nano-Ag were closely attached to Listeria monocytogenes but not to Salmonella E. There was no evidence of entering nano-Ag inside Listeria monocytogenes but smaller particles were placed inside Salmonella E. The ability of nano-D to attach to the flagella and the ability of nano-Ag to penetrate inside bacteria cells can be utilized to design nano-bacteria vehicles, being carriers for active substances attached to nanoparticles.
authors.affiliation: Warsaw University of Life Sciences (SGGW), 02-786 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Mitura
authors.forename: Stanisław
authors.initials: S
authors.lastname: Mitura
authors.forename: Jacek
authors.initials: J
authors.lastname: Szeliga
authors.forename: Tomasz
authors.initials: T
authors.lastname: Niemiec
authors.forename: Marlena
authors.initials: M
authors.lastname: Rupiewicz
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Sokołowska
mesh_terms: D018130:Diamond; D008089:Listeria monocytogenes / Q000187:drug effects*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D012477:Salmonella enteritidis / Q000187:drug effects*; D012834:Silver
chemical_list: D012834:Silver; D018130:Diamond