title: Compositional analysis and in vivo anti-diabetic activity of wild Algerian Marrubium vulgare L. infusion.
abstract: Marrubium vulgare (Lamiaceae) is a plant traditionally used for the treatment of diabetes in Algeria. Compositional analysis of the aqueous infusion revealed the presence of fifteen metabolites, all belonging to the class of polyphenols. Particularly, seven flavonoids have been detected, together with 5-caffeoylquinic (chlorogenic) acid in small amounts; the extract is dominated by the presence of a series of complex molecules, characterized as verbascoside (acteoside) derivatives. Concerning the anti-diabetic effectiveness a series of in vivo experiments were carried out on albinos Wistar rats. Diabetes was induced in the animals by intra-peritoneal injection of alloxane; they were treated twice a day with aqueous extract from aerial part infusion (100, 200 and 300 mg/kg body weight) and glibenclamide (5mg/kg body weight) for 15 days. Oral administration of 200 and 300 mg/kg body weight of aqueous extract the Marrubium vulgare induced an significant effect antidiabetic and antihyperlipidemic (dose-dependent effect). A decrease in blood glucose by 50% for the dose 100 mg/kg and more than 60% for doses 200 and 300 mg/kg, as well as a significant lowering of total lipids, triglycerides, and total cholesterol levels in treated animals, compared with diabetic controls group (p<0.001), have been observed. Glibenclamide was used as reference and showed similar effects.
authors.affiliation: Faculté des Sciences, Département des Sciences de la Nature et de la Vie, Université de M'Sila, M'Sila, Algeria. aboudjelal2003@yahoo.fr
authors.forename: Amel
authors.initials: A
authors.lastname: Boudjelal
authors.forename: Cherifa
authors.initials: C
authors.lastname: Henchiri
authors.forename: Laura
authors.initials: L
authors.lastname: Siracusa
authors.forename: Madani
authors.initials: M
authors.lastname: Sari
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ruberto
mesh_terms: D000462:Algeria; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D005905:Glyburide / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D008297:Male; D027522:Marrubium / Q000737:chemistry*; D008517:Phytotherapy; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D059808:Polyphenols; D005905:Glyburide