title: Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid.
abstract: Although glutathione S-transferases (GSTs) are thought to play major roles in oxidative stress metabolism, little is known about the regulatory functions of GSTs. We have reported that Arabidopsis (Arabidopsis thaliana) GLUTATHIONE S-TRANSFERASE U17 (AtGSTU17; At1g10370) participates in light signaling and might modulate various aspects of development by affecting glutathione (GSH) pools via a coordinated regulation with phytochrome A. Here, we provide further evidence to support a negative role of AtGSTU17 in drought and salt stress tolerance. When AtGSTU17 was mutated, plants were more tolerant to drought and salt stresses compared with wild-type plants. In addition, atgstu17 accumulated higher levels of GSH and abscisic acid (ABA) and exhibited hyposensitivity to ABA during seed germination, smaller stomatal apertures, a lower transpiration rate, better development of primary and lateral root systems, and longer vegetative growth. To explore how atgstu17 accumulated higher ABA content, we grew wild-type plants in the solution containing GSH and found that they accumulated ABA to a higher extent than plants grown in the absence of GSH, and they also exhibited the atgstu17 phenotypes. Wild-type plants treated with GSH also demonstrated more tolerance to drought and salt stresses. Furthermore, the effect of GSH on root patterning and drought tolerance was confirmed by growing the atgstu17 in solution containing l-buthionine-(S,R)-sulfoximine, a specific inhibitor of GSH biosynthesis. In conclusion, the atgstu17 phenotype can be explained by the combined effect of GSH and ABA. We propose a role of AtGSTU17 in adaptive responses to drought and salt stresses by functioning as a negative component of stress-mediated signal transduction pathways.
authors.affiliation: Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Jui-Hung
authors.initials: JH
authors.lastname: Chen
authors.forename: Han-Wei
authors.initials: HW
authors.lastname: Jiang
authors.forename: En-Jung
authors.initials: EJ
authors.lastname: Hsieh
authors.forename: Hsing-Yu
authors.initials: HY
authors.lastname: Chen
authors.forename: Ching-Te
authors.initials: CT
authors.lastname: Chien
authors.forename: Hsu-Liang
authors.initials: HL
authors.lastname: Hsieh
authors.forename: Tsan-Piao
authors.initials: TP
authors.lastname: Lin
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D055864:Droughts; D055786:Gene Knockout Techniques; D018525:Germination / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism* / Q000494:pharmacology; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D054046:Plant Stomata / Q000187:drug effects; D055049:Salt Tolerance; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D019328:Buthionine Sulfoximine; D000040:Abscisic Acid; D005982:Glutathione Transferase; D005978:Glutathione
references.citation: Plant Physiol. 1994 Sep;106(1):233-239
references.pmid: 12232324
references.citation: Plant Cell. 2008 Apr;20(4):1134-51
references.pmid: 18451323
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9437-42
references.pmid: 11481498
references.citation: Plant Physiol. 1997 Jan;113(1):93-102
references.pmid: 9008391
references.citation: Plant Physiol. 2007 Mar;143(3):1189-202
references.pmid: 17220357
references.citation: Plant J. 2006 Aug;47(3):343-55
references.pmid: 16792696
references.citation: Plant J. 1999 May;18(3):285-92
references.pmid: 10377994
references.citation: Trends Plant Sci. 2006 Sep;11(9):434-9
references.pmid: 16890475
references.citation: Methods Enzymol. 2005;401:169-86
references.pmid: 16399386
references.citation: J Exp Bot. 2009;60(4):1207-18
references.pmid: 19174456
references.citation: J Plant Physiol. 2009 Nov 15;166(17):1878-91
references.pmid: 19615785
references.citation: Plant Cell. 2000 Oct;12(10):1939-50
references.pmid: 11041888
references.citation: Plant Physiol. 2001 Nov;127(3):1147-56
references.pmid: 11706194
references.citation: Trends Plant Sci. 2000 May;5(5):193-8
references.pmid: 10785664
references.citation: Curr Genet. 1996 May;29(6):511-5
references.pmid: 8662189
references.citation: Plant Physiol. 1995 Nov;109(3):1047-57
references.pmid: 8552710
references.citation: Plant J. 2009 Oct;60(2):314-27
references.pmid: 19566594
references.citation: Mol Cell Biol. 2007 Jul;27(14):5214-24
references.pmid: 17485445
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19631-6
references.pmid: 18048328
references.citation: J Exp Bot. 2002 Jan;53(366):33-7
references.pmid: 11741038
references.citation: Plant Physiol. 2009 Nov;151(3):1433-45
references.pmid: 19759343
references.citation: J Plant Physiol. 2008 Sep 8;165(13):1390-403
references.pmid: 18171593
references.citation: J Plant Physiol. 2004 Mar;161(3):347-9
references.pmid: 15077634
references.citation: Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10580-4
references.pmid: 7479844
references.citation: J Exp Bot. 2004 Nov;55(407):2343-51
references.pmid: 15448181
references.citation: Plant Physiol. 1997 May;114(1):177-183
references.pmid: 12223697
references.citation: Plant Physiol. 1999 Mar;119(3):1091-100
references.pmid: 10069848
references.citation: Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15150-5
references.pmid: 17823244
references.citation: Biochem J. 2001 Nov 15;360(Pt 1):1-16
references.pmid: 11695986
references.citation: Plant Physiol. 2010 Dec;154(4):1646-58
references.pmid: 20935176
references.citation: Plant J. 2009 Apr;58(2):299-317
references.pmid: 19170934
references.citation: Mol Plant. 2008 Jan;1(1):42-57
references.pmid: 20031913
references.citation: J Exp Bot. 2008;59(11):2991-3007
references.pmid: 18552355
references.citation: Plant Cell. 2005 Jul;17(7):1866-75
references.pmid: 15987996
references.citation: Plant J. 2009 Apr;58(1):53-68
references.pmid: 19067976
references.citation: FEBS Lett. 2003 Oct 23;553(3):427-32
references.pmid: 14572664
references.citation: Antioxid Redox Signal. 2009 Apr;11(4):861-905
references.pmid: 19239350
references.citation: Plant Cell Physiol. 2000 Nov;41(11):1229-34
references.pmid: 11092907
references.citation: Plant Cell. 2000 Jan;12(1):97-110
references.pmid: 10634910
references.citation: Annu Rev Plant Biol. 2008;59:143-66
references.pmid: 18444899
references.citation: Planta. 2000 Sep;211(4):528-36
references.pmid: 11030552
references.citation: Plant Physiol. 2009 Jul;150(3):1394-410
references.pmid: 19482919
references.citation: Anal Biochem. 1980 Jul 15;106(1):207-12
references.pmid: 7416462
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:127-158
references.pmid: 15012285
references.citation: Mol Cell Proteomics. 2007 Dec;6(12):2058-71
references.pmid: 17848588
references.citation: Plant J. 2001 Feb;25(3):295-303
references.pmid: 11208021
references.citation: Biotechnol Lett. 2010 Aug;32(8):1173-9
references.pmid: 20383560
references.citation: Plant J. 2004 Jan;37(1):104-14
references.pmid: 14675436
references.citation: J Exp Bot. 2007;58(3):439-51
references.pmid: 17158109
references.citation: Plant Physiol. 2006 Nov;142(3):1065-74
references.pmid: 16963523
references.citation: Plant Physiol. 2007 Apr;143(4):1905-17
references.pmid: 17277097