title: Establishment of Epstein-Barr virus growth-transformed lymphoblastoid cell lines.
abstract: Infection of B cells with Epstein-Barr virus (EBV) leads to proliferation and subsequent immortalization, resulting in establishment of lymphoblastoid cell lines (LCL) in vitro. Since LCL are latently infected with EBV, they provide a model system to investigate EBV latency and virus-driven B cell proliferation and tumorigenesis(1). LCL have been used to present antigens in a variety of immunologic assays(2, 3). In addition, LCL can be used to generate human monoclonal antibodies(4, 5) and provide a potentially unlimited source when access to primary biologic materials is limited(6, 7). A variety of methods have been described to generate LCL. Earlier methods have included the use of mitogens such as phytohemagglutinin, lipopolysaccharide(8), and pokeweed mitogen(9) to increase the efficiency of EBV-mediated immortalization. More recently, others have used immunosuppressive agents such as cyclosporin A to inhibit T cell-mediated killing of infected B cells(7, 10-12). The considerable length of time from EBV infection to establishment of cell lines drives the requirement for quicker and more reliable methods for EBV-driven B cell growth transformation. Using a combination of high titer EBV and an immunosuppressive agent, we are able to consistently infect, transform, and generate LCL from B cells in peripheral blood. This method uses a small amount of peripheral blood mononuclear cells that are infected in vitroclusters of cells can be demonstrated. The presence of CD23 with EBV in the presence of FK506, a T cell immunosuppressant. Traditionally, outgrowth of proliferating B cells is monitored by visualization of microscopic clusters of cells about a week after infection with EBV. Clumps of LCL can be seen by the naked eye after several weeks. We describe an assay to determine early if EBV-mediated growth transformation is successful even before microscopic clusters of cells can be demonstrated. The presence of CD23(hi)CD58(+) cells observed as early as three days post-infection indicates a successful outcome.
authors.affiliation: Stony Brook Children's Hospital, State University of New York, USA.
authors.forename: Joyce
authors.initials: J
authors.lastname: Hui-Yuen
authors.forename: Shane
authors.initials: S
authors.lastname: McAllister
authors.forename: Siva
authors.initials: S
authors.lastname: Koganti
authors.forename: Erik
authors.initials: E
authors.lastname: Hill
authors.forename: Sumita
authors.initials: S
authors.lastname: Bhaduri-McIntosh
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology* / Q000821:virology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000502:physiology*; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000502:physiology*; D006801:Humans
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