title: The interaction of bovine serum albumin with doxorubicin-loaded superparamagnetic iron oxide nanoparticles: spectroscope and molecular modelling identification.
abstract: To take a comprehensive evaluation of the bio-safety of doxorubicin-loaded superparamagnetic iron oxide nanoparticles (SPION), the interaction of bovine serum albumin (BSA) with the drug delivery was investigated by multi-spectroscopic techniques and molecular modelling calculation. Ultraviolet absorption and synchronous fluorescence results elucidate that DOX-SPION unfold the framework conformation of BSA, leading to changes in the microenvironment of amide moieties. Circular dichroism (CD) data show that the content of α-helix decreases from 68.62% to 62.76%, which shows the changes of protein's secondary structure quantificationally. Through Stern-Volmer analysis, the quenching mode is determined to be static interaction, forming a stable bioconjugate. The molecular model illustrates that DOX prefers a highly polar binding site at the external region of domains □ of BSA, and the hydrogen bonds are marked. This work elucidates that the drug delivery has deleterious effects on the frame conformation of protein, affecting its physiological function.
authors.affiliation: Shandong University, Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, China -America CRC for Environment & Health, Shandong Province, Jinan 250100, P.R.China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Liu
authors.forename: Fanying
authors.initials: F
authors.lastname: Ji
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002417:Cattle; D002942:Circular Dichroism; D004317:Doxorubicin / Q000008:administration & dosage*; D005290:Ferric Compounds / Q000737:chemistry*; D053768:Metal Nanoparticles; D008958:Models, Molecular; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D005290:Ferric Compounds; C000499:ferric oxide; D012710:Serum Albumin, Bovine; D004317:Doxorubicin