title: MiRNA expression patterns predict survival in glioblastoma.
abstract: BACKGROUND
  In order to define new prognostic subgroups in patients with glioblastoma a miRNA screen (> 1000 miRNAs) from paraffin tissues followed by a bio-mathematical analysis was performed.
  
  
  METHODS
  35 glioblastoma patients treated between 7/2005 - 8/2008 at a single institution with surgery and postoperative radio(chemo)therapy were included in this retrospective analysis. For microarray analysis the febit biochip "Geniom® Biochip MPEA homo-sapiens" was used. Total RNA was isolated from FFPE tissue sections and 1100 different miRNAs were analyzed.
  
  
  RESULTS
  It was possible to define a distinct miRNA expression pattern allowing for a separation of distinct prognostic subgroups. The defined miRNA pattern was significantly associated with early death versus long-term survival (split at 450 days) (p = 0.01). The pattern and the prognostic power were both independent of the MGMT status.
  
  
  CONCLUSIONS
  At present, this is the first dataset defining a prognostic role of miRNA expression patterns in patients with glioblastoma. Having defined such a pattern, a prospective validation of this observation is required.
authors.affiliation: Department of Radiation Oncology, Ludwig-Maximilians-University Munich, Marchioninistr, 15, 81377 Munich, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Niyazi
authors.forename: Franz
authors.initials: F
authors.lastname: Zehentmayr
authors.forename: Olivier M
authors.initials: OM
authors.lastname: Niemöller
authors.forename: Sabina
authors.initials: S
authors.lastname: Eigenbrod
authors.forename: Hans
authors.initials: H
authors.lastname: Kretzschmar
authors.forename: Klaus
authors.initials: K
authors.lastname: Schulze-Osthoff
authors.forename: Jörg-Christian
authors.initials: JC
authors.lastname: Tonn
authors.forename: Mike
authors.initials: M
authors.lastname: Atkinson
authors.forename: Simone
authors.initials: S
authors.lastname: Mörtl
authors.forename: Claus
authors.initials: C
authors.lastname: Belka
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality; D016000:Cluster Analysis; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes
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