title: Caco-2 cell line as a model to evaluate mucoprotective proprieties.
abstract: Physical protection of mucosa surface and reduction of inflammatory processes are currently considered the main strategies in the treatment and prevention of mucosal diseases. However, the majority of models used to verify the activity of new mucoprotective agents are based on limiting instrumental assessment or the sacrifice of experimental animals. In this study, for the first time, some in vitro experimental methods using Caco-2 cell line are proposed as predicting in vivo behaviour and action of mucoprotective agents. To this purpose, hyaluronic acid and natural polysaccharides for their bioadhesive activity, hydrocortisone and natural polyphenols as anti-inflammatory agents have been chosen. The obtained results demonstrated that the techniques (Con A/o-pd assay and Franz cell system) of mucoadhesive evaluation on Caco-2 cells are useful to compare the activity of each experimental sample and to assess the adhesion time to the mucosal cell surface. Moreover, the reduction of intercellular adhesion molecule-1 (ICAM-1) expression in Caco-2 cells can be considered directly correlated to the mucosal anti-inflammatory effect induced by the hydrocortisone and natural polyphenols. In conclusion, the study supported the use of Caco-2 cell as a model to compare and investigate the effect of different active substances on the mucosa and its diseases.
authors.affiliation: Department of Pharmaceutical Sciences, University of Catania, V.le A. Doria 6, 95125 Catania, Italy. luisa.rizza@hotmail.it
authors.forename: L
authors.initials: L
authors.lastname: Rizza
authors.forename: G
authors.initials: G
authors.lastname: Frasca
authors.forename: M
authors.initials: M
authors.lastname: Nicholls
authors.forename: C
authors.initials: C
authors.lastname: Puglia
authors.forename: V
authors.initials: V
authors.lastname: Cardile
mesh_terms: D000268:Adhesiveness; D000826:Animal Testing Alternatives; D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology*; D001667:Binding, Competitive; D018938:Caco-2 Cells; D003208:Concanavalin A / Q000378:metabolism; D019610:Cytoprotection; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D059808:Polyphenols / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D059808:Polyphenols; D003208:Concanavalin A; D018799:Intercellular Adhesion Molecule-1; D006820:Hyaluronic Acid; D006854:Hydrocortisone