title: Selective killing of lung cancer cells using carcinoembryonic antigen promoter and double suicide genes, thymidine kinase and cytosine deaminase (pCEA-TK/CD).
abstract: The application of gene therapy in cancer treatment is limited by non-specific targeting. In the present study, we constructed a recombinant plasmid, containing a carcinoembryonic antigen (CEA) promoter and double suicide genes thymidine kinase (TK) and cytosine deaminase (CD), henceforth referred to as pCEA-TK/CD. Our results showed that the CEA promoter can specifically drive target gene expression in CEA-positive lung cancer cells. In the presence of prodrugs 5-flucytosine and ganciclovir, pCEA-TK/CD transfection decreased inhibitory concentration 50 and increased apoptosis and cyclomorphosis. Our result suggests that gene therapy using pCEA-TK/CD may be a promising new approach for treating lung cancer.
authors.affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangzhou Medical College, No. 151, Yanjiang Road, Guangzhou 510120, Guangdong, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qiu
authors.forename: Gui-Lin
authors.initials: GL
authors.lastname: Peng
authors.forename: Qi-Cai
authors.initials: QC
authors.lastname: Liu
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
authors.forename: Xu-Sen
authors.initials: XS
authors.lastname: Zou
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D043525:Cytosine Deaminase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D005437:Flucytosine / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics* / Q000628:therapy*; D010957:Plasmids / Q000235:genetics; D011355:Prodrugs / Q000493:pharmacokinetics / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D002272:Carcinoembryonic Antigen; D011355:Prodrugs; D005437:Flucytosine; D013937:Thymidine Kinase; D043525:Cytosine Deaminase; D015774:Ganciclovir