title: Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator.
abstract: In vivo optical imaging can reveal the dynamics of large-scale cortical activity, but methods for chronic recording are limited. Here we present a technique for long-term investigation of cortical map dynamics using wide-field ratiometric fluorescence imaging of the genetically encoded calcium indicator (GECI) Yellow Cameleon 3.60. We find that wide-field GECI signals report sensory-evoked activity in anaesthetized mouse somatosensory cortex with high sensitivity and spatiotemporal precision, and furthermore, can be measured repeatedly in separate imaging sessions over multiple weeks. This method opens new possibilities for the longitudinal study of stability and plasticity of cortical sensory representations.
authors.affiliation: Brain Research Institute, Department of Neurophysiology, University of Zurich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Minderer
authors.forename: Wenrui
authors.initials: W
authors.lastname: Liu
authors.forename: Lazar T
authors.initials: LT
authors.lastname: Sumanovski
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kügler
authors.forename: Fritjof
authors.initials: F
authors.lastname: Helmchen
authors.forename: David J
authors.initials: DJ
authors.lastname: Margolis
mesh_terms: D000818:Animals; D001931:Brain Mapping / Q000379:methods; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000229:Dependovirus / Q000235:genetics; D003952:Diagnostic Imaging / Q000379:methods; D005073:Evoked Potentials, Somatosensory / Q000502:physiology*; D005260:Female; D005453:Fluorescence; D008137:Longitudinal Studies; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D013003:Somatosensory Cortex / Q000378:metabolism / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; C495639:yellow cameleon; D002118:Calcium
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