title: cDNA display: rapid stabilization of mRNA display.
abstract: The cDNA display method is a robust in vitro display technology that converts an unstable mRNA-protein fusion (mRNA display) to a stable mRNA/cDNA-protein fusion (cDNA display) whose cDNA is covalently linked to its encoded protein using a well-designed puromycin linker. We provide technical details for preparing cDNA display molecules and for the synthesis of the puromycin linker for the purpose of screening the functional proteins and peptides.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Ueno
authors.forename: Naoto
authors.initials: N
authors.lastname: Nemoto
mesh_terms: D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019020:Directed Molecular Evolution; D011485:Protein Binding; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011691:Puromycin / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D011691:Puromycin