title: [Mechanism of ING4 mediated inhibition of the proliferation and migration of human glioma cell line U251].
abstract: OBJECTIVE
  To investigate the effect of Ad-ING4 on proliferation and migration of glioma cells and explore its probable mechanism.
  
  
  METHODS
  U251 were infected with Ad-ING4. ING4 gene expression was evaluated by RT-PCR. MTT assay was adopted to evaluate the effect of ING4 on proliferation of U251; Boyden chamber assay was used to check the effect of ING4 on the migration of U251. In ING4 transfected U251,  Western blot was used for detecting NGF and TrkA expression;  Pull-down assay was used for detecting active RhoA expression.
  
  
  RESULTS
  ING4 was overexpressed in Ad-ING4 transfected U251 cells. ING4 inhibited proliferation and migration of U251 significantly. Moreover,  overexpression of ING4 result in depression of NGF,  TrkA and active RhoA.
  
  
  CONCLUSIONS
  ING4 mediated inhibition of the proliferation and migration of human glioma cells by down regulating NGF,  TrkA and active RhoA expression.
authors.affiliation: Department of Neurosurgery,  The Affiliated People's Hospital of Jiangsu University,  Zhenjiang  212002,  China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhan
authors.forename: Qiao-yu
authors.initials: QY
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Zhang
authors.forename: Zhi-cheng
authors.initials: ZC
authors.lastname: Yuan
authors.forename: Pei-song
authors.initials: PS
authors.lastname: Lu
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D016543:Central Nervous System Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015536:Down-Regulation; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D018398:Homeodomain Proteins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D020932:Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism; D020917:Receptor, trkA / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000378:metabolism* / Q000494:pharmacology; D020742:rhoA GTP-Binding Protein / Q000187:drug effects / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D025521:Tumor Suppressor Proteins; D020932:Nerve Growth Factor; D020917:Receptor, trkA; D020742:rhoA GTP-Binding Protein