title: A novel in situ probe for oxygen uptake rate measurement in mammalian cell cultures.
abstract: The newly developed in situ oxygen uptake rate (in situ OUR) probe presented in this article is based on the in situ microscope technology platform. It is designed to measure the oxygen uptake rate (OUR) of mammalian cells, an important parameter for metabolic flux analysis, inside a reactor (in situ) and in real-time. The system isolates a known volume of cell culture from the bulk inside the bioreactor, monitors the oxygen consumption over time, and releases the sample again. The sample is mixed during the measurement with a new agitation system to keep the cells in suspension and prevent oxygen concentration gradients. The OUR measurement system also doubles as a standard dissolved oxygen (DO) probe for process monitoring when it is not performing OUR measurements. It can be equipped with two different types of optical sensors (i.e., DO, pH) simultaneously or a conventional polarographic DO-probe (Clark type). This new probe was successfully tested in baby hamster kidney perfusion cell cultures.
authors.affiliation: Leibniz University Hanover, Institute for Technical Chemistry, Callinstr. 3, 30167 Hannover, Germany.
authors.forename: Larissa
authors.initials: L
authors.lastname: Behr
authors.forename: Klaus
authors.initials: K
authors.lastname: Joeris
authors.forename: Mark
authors.initials: M
authors.lastname: Burnett
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002477:Cells / Q000737:chemistry / Q000378:metabolism*; D006224:Cricetinae; D007700:Kinetics; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D010100:Oxygen