title: A gustatory receptor involved in host plant recognition for oviposition of a             swallowtail butterfly.
abstract: Swallowtail butterflies belonging to the family of Papilionidae selectively                 utilize a limited number of plants from a single or a few families. Female                 butterflies lay eggs on their host only when they detect specific chemicals through                 their foreleg chemosensilla while drumming on the leaf surface. Here we show that                 the butterfly, Papilio xuthus, uses a gustatory receptor specific for                 synephrine to select its host in oviposition behaviour. We identify a gustatory                 receptor gene involved in the recognition of an oviposition stimulant, synephrine,                 from the P. xuthus by a combination of in silico, in vitro and                     in vivo approaches. The receptor, PxutGr1, responds specifically to                 synephrine in Sf9 cells. The sensitivity of tarsal taste sensilla to synephrine and                 the oviposition behaviour in response to synephrine are strongly reduced after                 injecting double-stranded RNA of PxutGr1 into pupae. These observations                 indicate that the receptor PxutGr1 represents a key factor in host specialization in                     P. xuthus.
authors.affiliation: JT Biohistory Research Hall, Takatsuki, Osaka, Japan. ozk.kths@gmail.com
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Ozaki
authors.forename: Masasuke
authors.initials: M
authors.lastname: Ryuda
authors.forename: Ayumi
authors.initials: A
authors.lastname: Yamada
authors.forename: Ai
authors.initials: A
authors.lastname: Utoguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishimoto
authors.forename: Delphine
authors.initials: D
authors.lastname: Calas
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marion-Poll
authors.forename: Teiichi
authors.initials: T
authors.lastname: Tanimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002080:Butterflies; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D010058:Oviposition / Q000187:drug effects*; D012330:RNA, Double-Stranded / Q000235:genetics; D013578:Synephrine / Q000494:pharmacology
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; D013578:Synephrine
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