title: Biological functionalization of dental implants with collagen and glycosaminoglycans-A comparative study.
abstract: Biological implant surface coatings are an emerging technology to increase bone formation. Such an approach is of special interest in anatomical regions like the maxilla. In the present study, we hypothesized that the coating of titanium implants with components of the organic extracellular matrix increases bone formation and implant stability compared to an uncoated reference. The implants were coated using collagen-I with either two different concentrations of chondroitin sulfate (CS) or two differentially sulfated hyaluronans. Implant coatings were characterized biochemically and with atomic force microscopy. Histomorphometry was used to assess bone-implant contact (BIC) and bone-volume density (BVD) after 4 and 8 weeks of submerged healing in the maxilla of 20 minipigs. Further, implant stability was measured by resonance frequency analysis (RFA). Implants containing the lower CS concentration had significantly more BIC, compared to the uncoated reference at both times of interest. No significant increase was measured from week 4 to 8. Differences in BVD and RFA were statistically not significant. A higher concentration of CS and the application of sulfated hyaluronans showed no comparable increase in BIC. This study demonstrates a positive effect of a specific collagen-glycosaminoglycan combination on early bone formation in vivo.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University of Technology Dresden, Faculty of Medicine, Fetscherstr. 74, D-01307 Dresden, Germany. stadlinger@gmx.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Stadlinger
authors.forename: Vera
authors.initials: V
authors.lastname: Hintze
authors.forename: Susanne
authors.initials: S
authors.lastname: Bierbaum
authors.forename: Stephanie
authors.initials: S
authors.lastname: Möller
authors.forename: Matthias C
authors.initials: MC
authors.lastname: Schulz
authors.forename: Ronald
authors.initials: R
authors.lastname: Mai
authors.forename: Eberhard
authors.initials: E
authors.lastname: Kuhlisch
authors.forename: Sascha
authors.initials: S
authors.lastname: Heinemann
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.forename: Matthias
authors.initials: M
authors.lastname: Schnabelrauch
authors.forename: Uwe
authors.initials: U
authors.lastname: Eckelt
mesh_terms: D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D015921:Dental Implants; D005260:Female; D008422:Materials Testing; D013552:Swine; D013556:Swine, Miniature
chemical_list: D024042:Collagen Type I; D015921:Dental Implants; D002809:Chondroitin Sulfates