title: Vid30 is required for the association of Vid vesicles and actin patches in the vacuole import and degradation pathway.
abstract: When Saccharomyces cerevisiae is starved of glucose, the gluconeogenic enzymes fructose-1,6-bisphosphatase (FBPase), malate dehydrogenase (MDH2), isocitrate lyase (Icl1) and phosphoenolpyruvate carboxykinase (Pck1) are induced. However, when glucose is added to prolonged starved cells, these enzymes are degraded in the vacuole via the vacuole import and degradation (Vid) pathway. Recent evidence suggests that the Vid pathway merges with the endocytic pathway at actin patches where endocytic vesicles are formed. The convergence of the Vid pathway with the endocytic pathway allows cells to remove intracellular and extracellular proteins simultaneously. However, the genes that regulate this step of the convergence have not been identified previously. Here we show that VID30 plays a critical role for the association of Vid vesicles and actin patches. Vid30 is constitutively expressed and interacts with Vid vesicle proteins Vid24 and Sec28 but not with the cargo protein FBPase. In the absence of SEC28 or VID24, Vid30 association with actin patches was prolonged. In cells lacking the VID30 gene, FBPase and Vid24 were not localized to actin patches, suggesting that Vid30 has a role in the association of Vid vesicles and actin patches. Vid30 contains a LisH and a CTLH domain, both of which are required for FBPase degradation. When these domains were deleted, FBPase trafficking to the vacuole was impaired. We suggest that Vid30 also has a role in the Vid pathway at a later step in a process that is mediated by the LisH and CTLH domains.
authors.affiliation: Department of Cellular and Molecular Physiology, Penn State University, College of Medicine, Hershey, PA, USA.
authors.forename: Abbas A
authors.initials: AA
authors.lastname: Alibhoy
authors.forename: Bennett J
authors.initials: BJ
authors.lastname: Giardina
authors.forename: Danielle D
authors.initials: DD
authors.lastname: Dunton
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Chiang
mesh_terms: D000199:Actins / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D022161:Transport Vesicles / Q000187:drug effects / Q000378:metabolism*; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000199:Actins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; C434473:Vid30 protein, S cerevisiae; D049452:Green Fluorescent Proteins; D006597:Fructose-Bisphosphatase; D005947:Glucose
references.citation: Curr Pharm Des. 2010 Jan;16(1):101-13
references.pmid: 20214621
references.citation: Biochem Biophys Res Commun. 2005 Oct 7;335(4):1185-90
references.pmid: 16112084
references.citation: J Biol Chem. 1996 Apr 26;271(17):9934-41
references.pmid: 8626630
references.citation: PLoS Genet. 2010 Feb 05;6(2):e1000838
references.pmid: 20140187
references.citation: Semin Cell Dev Biol. 2010 Sep;21(7):691-8
references.pmid: 20188203
references.citation: J Bacteriol. 1995 Oct;177(19):5622-7
references.pmid: 7559351
references.citation: Neurosci Lett. 2009 May 1;454(3):203-8
references.pmid: 19429084
references.citation: Revis Biol Celular. 1989;21:305-19
references.pmid: 2561496
references.citation: Dev Cell. 2008 Sep;15(3):344-357
references.pmid: 18804433
references.citation: Biol Rev Camb Philos Soc. 2009 Aug;84(3):431-48
references.pmid: 19659885
references.citation: EMBO J. 2000 May 15;19(10):2161-7
references.pmid: 10811607
references.citation: Open Biochem J. 2010 Jan 13;4:1-8
references.pmid: 20224683
references.citation: Am J Pathol. 1995 Oct;147(4):1142-51
references.pmid: 7573359
references.citation: Gene. 2007 Jul 15;396(2):236-47
references.pmid: 17467196
references.citation: Microbiol Mol Biol Rev. 1998 Jun;62(2):334-61
references.pmid: 9618445
references.citation: J Biol Chem. 2010 Jan 8;285(2):1516-28
references.pmid: 19892709
references.citation: J Cell Biol. 2003 Sep 1;162(5):765-72
references.pmid: 12952930
references.citation: Curr Opin Cell Biol. 2010 Apr;22(2):124-31
references.pmid: 20034776
references.citation: J Biol Chem. 2002 Mar 8;277(10):8248-54
references.pmid: 11773046
references.citation: Curr Genet. 2006 Aug;50(2):101-14
references.pmid: 16741702
references.citation: Mol Biol Cell. 2003 Apr;14(4):1652-63
references.pmid: 12686616
references.citation: Hum Mol Genet. 1997 Feb;6(2):147-55
references.pmid: 9063734
references.citation: J Cell Biol. 1998 Mar 23;140(6):1347-56
references.pmid: 9508768
references.citation: FEBS Lett. 1994 Aug 1;349(2):270-4
references.pmid: 8050580
references.citation: Cell. 2008 Jan 11;132(1):27-42
references.pmid: 18191218
references.citation: Am J Med Genet. 1984 Jul;18(3):509-26
references.pmid: 6476009
references.citation: Arch Biochem Biophys. 1994 Dec;315(2):302-9
references.pmid: 7986072
references.citation: J Biol Chem. 1995 Nov 3;270(44):26446-50
references.pmid: 7592860
references.citation: Nat Rev Mol Cell Biol. 2009 Jul;10(7):458-67
references.pmid: 19491929
references.citation: J Biol Chem. 2004 Nov 19;279(47):49138-50
references.pmid: 15358789
references.citation: J Cell Biol. 1995 Mar;128(5):779-92
references.pmid: 7533169
references.citation: FEBS Lett. 2010 Apr 2;584(7):1417-26
references.pmid: 20096689
references.citation: J Biol Chem. 2007 Mar 2;282(9):6763-72
references.pmid: 17192262
references.citation: J Biol Chem. 2001 Dec 21;276(51):48017-26
references.pmid: 11641409
references.citation: Annu Rev Cell Dev Biol. 2003;19:287-332
references.pmid: 14570572
references.citation: Mol Biol Cell. 2008 Aug;19(8):3323-33
references.pmid: 18508925
references.citation: J Cell Biol. 2000 Jul 10;150(1):65-76
references.pmid: 10893257
references.citation: J Biol Chem. 2001 Aug 3;276(31):28659-66
references.pmid: 11356843
references.citation: Nat Struct Mol Biol. 2005 Apr;12(4):294-303
references.pmid: 15778719
references.citation: Hum Mol Genet. 1997 Feb;6(2):157-64
references.pmid: 9063735
references.citation: J Pathol. 2010 May;221(1):3-12
references.pmid: 20225336
references.citation: J Cell Sci. 2002 Feb 1;115(Pt 3):655-66
references.pmid: 11861771
references.citation: Semin Cell Dev Biol. 2010 Sep;21(7):719-26
references.pmid: 20176123
references.citation: Biochem Biophys Res Commun. 1987 Feb 27;143(1):212-7
references.pmid: 3030316
references.citation: J Biol Chem. 1998 Feb 6;273(6):3381-7
references.pmid: 9452458
references.citation: Curr Opin Microbiol. 1999 Apr;2(2):202-7
references.pmid: 10322167
references.citation: J Cell Biol. 1997 Feb 24;136(4):803-10
references.pmid: 9049246
references.citation: Methods Enzymol. 2009;453:111-44
references.pmid: 19216904
references.citation: J Biol Chem. 2008 Sep 19;283(38):26116-27
references.pmid: 18660504
references.citation: J Biol Chem. 2001 Mar 30;276(13):10398-406
references.pmid: 11134048
references.citation: Curr Opin Cell Biol. 2009 Feb;21(1):20-7
references.pmid: 19186047
references.citation: Autophagy. 2007 Sep-Oct;3(5):417-21
references.pmid: 17534141
references.citation: Brain. 2010 Jan;133(Pt 1):93-104
references.pmid: 20007218
references.citation: Cell. 2003 Nov 14;115(4):475-87
references.pmid: 14622601
references.citation: Eur J Biochem. 1994 Jun 15;222(3):879-84
references.pmid: 8026498
references.citation: J Biol Chem. 2010 Apr 9;285(15):11061-7
references.pmid: 20147746
references.citation: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Oct 1;62(Pt 10):1021-3
references.pmid: 17012802
references.citation: Cell Cycle. 2007 Aug 1;6(15):1837-49
references.pmid: 17671424
references.citation: FEMS Yeast Res. 2008 Mar;8(2):204-16
references.pmid: 17986252
references.citation: Nat Rev Drug Discov. 2007 Apr;6(4):304-12
references.pmid: 17396135
references.citation: Nature. 1991 Mar 28;350(6316):313-8
references.pmid: 1848921
references.citation: J Biol Chem. 1998 Sep 25;273(39):25000-5
references.pmid: 9737955
references.citation: Biol Chem. 2008 Sep;389(9):1143-51
references.pmid: 18713001
references.citation: J Bacteriol. 1971 Aug;107(2):401-5
references.pmid: 4329729
references.citation: Annu Rev Nutr. 2007;27:19-40
references.pmid: 17311494
references.citation: EMBO Rep. 2010 Jan;11(1):3
references.pmid: 20033083
references.citation: Neuropathol Appl Neurobiol. 2010 Apr;36(2):125-32
references.pmid: 20202120
references.citation: Mol Cell Biol. 2010 Feb;30(4):1049-58
references.pmid: 19995911
references.citation: J Biol Chem. 1992 Aug 25;267(24):17458-64
references.pmid: 1324938
references.citation: Annu Rev Genet. 1996;30:405-39
references.pmid: 8982460
references.citation: Nat Rev Mol Cell Biol. 2007 Nov;8(11):931-7
references.pmid: 17712358