title: Expressed sequenced tags profiling of resistant and  susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus.
abstract: Tick resistance in cattle is mainly found in zebu (Bos  indicus) animals, although it is also present in some taurine (B. taurus)  breeds. In order to characterize functional genes involved in tick  resistance/susceptibility in cattle, two cDNA libraries were generated using  skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality  reads from both resistant and susceptible cDNA were assembled into 458  sequences (contigs) and 834 singletons, with a mean size of 447.7 nucleotides.  Assignment of homologous proteins by BLASTX revealed 790 (61.1%) and 300  (23.2%) hits in resistant and susceptible cDNA, respectively; 121 of these hits  matched bovine proteins. A total of 502 (38.9%) unique sequences were found to  have no significant homology with known sequences and were classified as novel  sequences. In general, the most abundant sequences consisted of those coding  for hypothetical proteins whose function had not yet been determined, in  addition to ribosomal proteins, binding proteins and structural proteins, such  as keratin and collagen. The most abundant protein found was collagen type III  alpha, although ribosomal proteins accounted for half of the 40 most frequent  hits. In addition, five matches within the top 40 best hits corresponded to  immune response proteins. These sequences could be used for future studies on  functional genomics of cattle tick resistance as well as for genomic sequencing  projects.
authors.affiliation: Departamento de Zootecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: C S
authors.initials: CS
authors.lastname: Nascimento
authors.forename: M A
authors.initials: MA
authors.lastname: Machado
authors.forename: S E F
authors.initials: SE
authors.lastname: Guimarães
authors.forename: M F
authors.initials: MF
authors.lastname: Martins
authors.forename: J O
authors.initials: JO
authors.lastname: Peixoto
authors.forename: J
authors.initials: J
authors.lastname: Furlong
authors.forename: M C A
authors.initials: MC
authors.lastname: Prata
authors.forename: R S
authors.initials: RS
authors.lastname: Verneque
authors.forename: R L
authors.initials: RL
authors.lastname: Teodoro
authors.forename: P S
authors.initials: PS
authors.lastname: Lopes
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000469:parasitology; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology; D016384:Consensus Sequence / Q000235:genetics; D003433:Crosses, Genetic; D030562:Databases, Protein; D060467:Disease Resistance / Q000235:genetics*; D004198:Disease Susceptibility / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D015723:Gene Library; D008297:Male; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D048494:Rhipicephalus / Q000502:physiology*; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D012333:RNA, Messenger