title: Capillary electrophoretic determination of DNA damage markers: content of 8-hydroxy-2'-deoxyguanosine and 8-nitroguanine in urine.
abstract: A sensitive and low-cost analytical method has been developed to determine 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-nitroguanine (8-NO(2)Gua) based on capillary electrophoresis with amperometric detection (CE-AD) after solid phase extraction (SPE). Under optimized condition, these two markers were well separated from other components coexisting in urine, exhibiting a linear calibration over the concentration range of 0.1-50.0 μg/mL with the detection limits ranging from 0.02 to 0.06 μg/mL. The relative standard deviations (RSDs) were in the range of 0.1-2.1% for peak area, 0.1-1.5% for migration time, respectively. The average recovery and RSD were within the range of 100.0-108.0% and 0.1-1.7%, respectively. It was found that the urinary contents of 8-OHdG and 8-NO(2)Gua in cancer patients were significantly higher than those in healthy ones.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai, China.
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Li
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Zhang
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Li
authors.forename: Qing-Cui
authors.initials: QC
authors.lastname: Chu
authors.forename: Jian-Nong
authors.initials: JN
authors.lastname: Ye
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000652:urine; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: C095838:8-nitroguanine; D015415:Biomarkers; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine