title: Rankl-induced osteoclastogenesis leads to loss of mineralization in a medaka osteoporosis model.
abstract: Osteoclasts are macrophage-related bone resorbing cells of hematopoietic origin. Factors that regulate osteoclastogenesis are of great interest for investigating the pathology and treatment of bone diseases such as osteoporosis. In mammals, receptor activator of NF-κB ligand (Rankl) is a regulator of osteoclast formation and activation: its misexpression causes osteoclast stimulation and osteoporotic bone loss. Here, we report an osteoporotic phenotype that is induced by overexpression of Rankl in the medaka model. We generated transgenic medaka lines that express GFP under control of the cathepsin K promoter in osteoclasts starting at 12 days post-fertilization (dpf), or Rankl together with CFP under control of a bi-directional heat-shock promoter. Using long-term confocal time-lapse imaging of double and triple transgenic larvae, we monitored in vivo formation and activation of osteoclasts, as well as their interaction with osteoblasts. Upon Rankl induction, GFP-positive osteoclasts are first observed in the intervertebral regions and then quickly migrate to the surface of mineralized neural and haemal arches, as well as to the centra of the vertebral bodies. These osteoclasts are TRAP (tartrate-resistant acid phosphatase) and cathepsin K positive, mononuclear and highly mobile with dynamically extending protrusions. They are exclusively found in tight contact with mineralized matrix. Rankl-induced osteoclast formation resulted in severe degradation of the mineralized matrix in vertebral bodies and arches. In conclusion, our in vivo imaging approach confirms a conserved role of Rankl in osteoclastogenesis in teleost fish and provides new insight into the cellular interactions during bone resorption in an animal model that is useful for genetic and chemical screening.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore 117543.
authors.forename: Thuy Thanh
authors.initials: TT
authors.lastname: To
authors.forename: P Eckhard
authors.initials: PE
authors.lastname: Witten
authors.forename: Joerg
authors.initials: J
authors.lastname: Renn
authors.forename: Dipanjan
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Ann
authors.initials: A
authors.lastname: Huysseune
authors.forename: Christoph
authors.initials: C
authors.lastname: Winkler
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001862:Bone Resorption / Q000503:physiopathology*; D056657:Cathepsin K / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D018613:Microscopy, Confocal; D009990:Oryzias; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D010024:Osteoporosis / Q000503:physiopathology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D053245:RANK Ligand / Q000378:metabolism*; D000071681:Tartrate-Resistant Acid Phosphatase; D059008:Time-Lapse Imaging
chemical_list: D007527:Isoenzymes; D053245:RANK Ligand; D049452:Green Fluorescent Proteins; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D056657:Cathepsin K