title: Association of interleukin-10 gene promoter polymorphisms with chronic and aggressive periodontitis.
abstract: OBJECTIVE
  Interleukin-10 gene promoter polymorphisms have been associated with interleukin-10 decreased production, thereby playing a role in the pathogenesis of periodontitis. This study aimed to investigate whether interleukin-10 single nucleotide polymorphisms at positions -1087(G/A) and -597(C/A) are associated with generalised chronic periodontitis and localised aggressive periodontitis.
  
  
  METHODS
  Genomic DNA samples were isolated from 276 unrelated Jordanian participants. Subjects were categorised into 86 periodontally healthy controls, 105 chronic periodontitis patients and 85 localised aggressive periodontitis patients. Genotype frequencies were calculated, and differences were determined using Pearson chi-squared test, and odds ratio and 95% confidence intervals were included.
  
  
  RESULTS
  The frequencies of the -1087A and -597A alleles were significantly more common in chronic periodontitis patients than controls. The A-positive allele genotypes (GA, AA) at position -1087 and A-positive allele genotypes (CA, AA) at position -597 appeared to increase the risk of having chronic periodontitis. No significant differences were observed in the genotype frequencies between localised aggressive periodontitis patients and controls.
  
  
  CONCLUSIONS
  These findings indicate the possible use of interleukin-10 single nucleotide polymorphisms as genetic markers in chronic periodontitis patients and further emphasise the molecular differences between chronic periodontitis and aggressive periodontitis.
authors.affiliation: Klinik für Hautkrankheiten, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, Jena, Germany. Sameh.Jaradat@med.uni-jena.de
authors.forename: S M
authors.initials: SM
authors.lastname: Jaradat
authors.forename: K T
authors.initials: KT
authors.lastname: Ababneh
authors.forename: S A
authors.initials: SA
authors.lastname: Jaradat
authors.forename: M S
authors.initials: MS
authors.lastname: Abbadi
authors.forename: A H
authors.initials: AH
authors.lastname: Taha
authors.forename: J A
authors.initials: JA
authors.lastname: Karasneh
authors.forename: H I
authors.initials: HI
authors.lastname: Haddad
mesh_terms: D000225:Adenine; D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000276:immunology; D000483:Alleles; D055113:Chronic Periodontitis / Q000235:genetics* / Q000276:immunology; D003596:Cytosine; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006147:Guanine; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors
chemical_list: D005819:Genetic Markers; D016753:Interleukin-10; D006147:Guanine; D003596:Cytosine; D000225:Adenine