title: HBV inhibits apoB production via the suppression of MTP expression.
abstract: BACKGROUND
  Liver dominates the production and secretion of apolipoprotein B (apoB) and evidence shows that liver malfunction induced by hepatitis B virus (HBV) infection could lead to apolipoprotein metabolism disorders. The present study was undertaken to assess the effects of HBV on apoB expression.
  
  
  METHODS
  Clinical examination: serum apoB levels in patients with chronic HBV infection and in healthy individuals were measured by immunoturbidimetry using biochemical analyzer Olympus 5400. Cell study: mRNA and protein expression levels of apoB in HepG2 and HepG2.2.15 cells were measured by RT-PCR and Western blot. Alternatively, HBV infectious clone pHBV1.3 or control plasmid pBlue-ks were tranfected into HepG2 cells, and mRNA and protein expression levels of apoB, as well as the microsomal triglyceride transfer protein (MTP) in tranfected HepG2 cells were also measured by RT-PCR and western blot.
  
  
  RESULTS
  Serum apoB level was much lower in chronic HBV patients as compared to healthy individuals (P < 0.05). Expression of apoB mRNA and protein was lower in HepG2.2.15 cells than in HepG2 cells. Similarly, expression of apoB mRNA and protein was lower in pHBV1.3 transfected HepG2 cells than in pBlue-ks transfected HepG2 cells. Expression of MTP mRNA and protein in pHBV1.3 transfected HepG2 cells was reduced in a dose-dependent fashion.
  
  
  CONCLUSIONS
  HBV infection plays an inhibitory effect on apoB expression.
authors.affiliation: Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan, 430071, PR China
authors.forename: Fu-Bing
authors.initials: FB
authors.lastname: Wang
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Zhu
authors.forename: Xinghui
authors.initials: X
authors.lastname: Liu
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Gao
mesh_terms: D001055:Apolipoproteins B / Q000097:blood*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D015870:Gene Expression; D056945:Hep G2 Cells; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D001055:Apolipoproteins B; D002352:Carrier Proteins; D012333:RNA, Messenger; C106092:microsomal triglyceride transfer protein
references.citation: Virol J. 2010 Sep 14;7:228
references.pmid: 20840785
references.citation: J Lipid Res. 2003 Jan;44(1):22-32
references.pmid: 12518019
references.citation: Hepatology. 2010 Sep;52(3):854-63
references.pmid: 20690192
references.citation: Cancer Lett. 2009 Dec 1;286(1):69-79
references.pmid: 19201080
references.citation: Clin Lab Med. 2006 Dec;26(4):733-50
references.pmid: 17110237
references.citation: PLoS One. 2008;3(11):e3771
references.pmid: 19020660
references.citation: J Pathol. 2009 Oct;219(2):253-62
references.pmid: 19621337
references.citation: J Biol Chem. 2002 Aug 30;277(35):31516-25
references.pmid: 12072432
references.citation: Biochem Soc Trans. 2004 Feb;32(Pt 1):59-64
references.pmid: 14748713
references.citation: Biol Cell. 2009 Oct 28;102(1):63-74
references.pmid: 19857204
references.citation: J Intern Med. 2005 Nov;258(5):395-410
references.pmid: 16238675
references.citation: Curr Opin Lipidol. 1999 Aug;10(4):341-6
references.pmid: 10482137
references.citation: J Clin Virol. 2005 Dec;34 Suppl 1:S75-8
references.pmid: 16461228
references.citation: Clin Immunol. 2009 Apr;131(1):92-7
references.pmid: 19081304
references.citation: Gastroenterology. 2004 Feb;126(2):586-97
references.pmid: 14762795
references.citation: Lipids Health Dis. 2011 Aug 29;10:154
references.pmid: 21875437
references.citation: J Lipid Res. 2004 Jul;45(7):1169-96
references.pmid: 15102878
references.citation: Biochemistry. 1997 Oct 21;36(42):13060-7
references.pmid: 9335568
references.citation: Clin Chim Acta. 2009 Mar;401(1-2):124-7
references.pmid: 19083997
references.citation: Antivir Ther. 2010;15 Suppl 3:15-24
references.pmid: 21041900
references.citation: Hepatology. 2009 May;49(5 Suppl):S45-55
references.pmid: 19399792
references.citation: J Virol. 2003 May;77(9):5503-6
references.pmid: 12692252
references.citation: Gastroenterology. 2004 Nov;127(5 Suppl 1):S303-9
references.pmid: 15508098
references.citation: Immunol Cell Biol. 2007 Jan;85(1):16-23
references.pmid: 17130898
references.citation: J Viral Hepat. 2006 Jan;13(1):56-61
references.pmid: 16364083
references.citation: J Biol Chem. 2004 Jul 2;279(27):28106-12
references.pmid: 15123606