title: Transcription analysis of lignocellulolytic enzymes of Penicillium decumbens 114-2 and its catabolite-repression-resistant mutant.
abstract: Penicillium decumbens 114-2 is a fast-growing filamentous fungus which secretes a variety of lignocellulolytic enzymes. Its catabolite-repression-resistant mutant JU-A10 with high secretion capacity of cellulolytic enzymes has been used industrially for biomass hydrolysis. Transcription levels of 6 important lignocellulolytic enzymes genes (cel5A, cel6A, cel7A, cel7B, xyn10A, and xyn11A) from both strains were determined on different carbon sources (glucose, sorbose, lactose, cellobiose, cellulose, and cellulose-wheat bran), by means of a real-time quantitative polymerase chain reaction. For both strains, the 6 genes are coordinately regulated at transcriptional level. Glucose and cellobiose repressed whereas cellulose and cellulose-wheat bran induced expression of 6 genes in both strains. Expression levels of all genes tested in the mutant strain JU-A10 were substantially higher than those in wild-type strain 114-2 on all carbon sources. On glucose repression condition, the mutant JU-A10 appeared obviously derepressed. Lactose was first proved to have an inductive effect on lignocellulolytic enzyme genes expression at lower concentration in Penicillium spp.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wei
authors.forename: Kai
authors.initials: K
authors.lastname: Zheng
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lei
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Qin
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004043:Dietary Fiber / Q000032:analysis; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005947:Glucose / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics; D007785:Lactose / Q000737:chemistry; D008031:Lignin / Q000737:chemistry*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D018749:RNA, Plant / Q000096:biosynthesis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004043:Dietary Fiber; D009005:Monosaccharides; D018749:RNA, Plant; C036909:lignocellulose; D008031:Lignin; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; D005947:Glucose; D007785:Lactose