title: Human leukocyte antigen epitope analysis to assess complement- and non-complement-binding donor-specific antibody repertoire in a pediatric heart transplant recipient.
abstract: This case report summarizes the spectrum of anti-human leukocyte antigen (HLA) antibody reactivity determined by single-allele Luminex immunoglobulin G and C1q binding assays before transplant, during an episode of antibody-mediated rejection (AMR), and following treatment in a sensitized pediatric heart transplant (Tx) recipient. We were able to discriminate between complement- and non-complement-binding epitope-specific antibodies present against a single donor antigen (HLA-A2) during the progression of AMR and its resolution. Our findings illustrate the usefulness of determining antibody specificities against epitopes using various Luminex-based assays.
authors.affiliation: University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA. zeevia@upmc.edu
authors.forename: Adriana
authors.initials: A
authors.lastname: Zeevi
authors.forename: Marilyn
authors.initials: M
authors.lastname: Marrari
authors.forename: Brian
authors.initials: B
authors.lastname: Feingold
authors.forename: Steven
authors.initials: S
authors.lastname: Webber
authors.forename: Rene J
authors.initials: RJ
authors.lastname: Duquesnoy
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015922:Complement C1q / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D013997:Time Factors
chemical_list: D000906:Antibodies; D000939:Epitopes; D015789:HLA-A2 Antigen; D007074:Immunoglobulin G; D015922:Complement C1q