title: Comparative and evolutionary analysis of new variants of ω-gliadin genes from three A-genome diploid wheats.
abstract: A genomic polymerase chain reaction (PCR) cloning strategy was applied to isolate ω-gliadin sequences from three A-genome diploid wheats (Triticum monococcum, T. boeoticum and T. urartu). Amplicon lengths varied from 744 and 1,044 bp, and those of the corresponding deduced mature proteins from 248 to 348 residues. The primary structure of the deduced polypeptides comprised a short N- and C-terminal conserved domain, and a long, variable repetitive domain. A phylogenetic analysis recognised several clades: the first consisted of three T. aestivum sequences; the second and the third two T. boeoticum and six T. monococcum sequences; and the rest four T. urartu and three T. aestivum sequences. Among the functional (non-pseudogene) ARQ/E-type ω-gliadin sequences, two were derived from T. boeoticum and three from T. monococcum; one of the latter sequences appeared to be a chimera originating via illegitimate recombination between the other two T. monococcum sequences. None of the 12 intact ω-gliadin sequences contained any cysteine or methionine residues. We discussed the variation and evolution of A-genome ω-gliadin genes.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100 People's Republic of China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhuang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fanguo
authors.initials: F
authors.lastname: Chen
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D005075:Biological Evolution; D004171:Diploidy; D014644:Genetic Variation; D018745:Genome, Plant; D005903:Gliadin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D050506:Mutant Chimeric Proteins / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D014908:Triticum / Q000145:classification / Q000235:genetics*
chemical_list: D050506:Mutant Chimeric Proteins; D010455:Peptides; D020033:Protein Isoforms; D005903:Gliadin
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