title: Ubiquitous expression of selenoprotein N transcripts in chicken tissues and early developmental expression pattern in skeletal muscles.
abstract: Previous results revealed a ubiquitous expression pattern of selenoprotein N (SelN, SEPN1) in humans, zebrafish, and mouse, suggesting that it plays a potential role during the embryogenesis of these species. However, no information is known about the tissue distribution of SelN and mRNA expression analysis in the muscle tissues during development in birds. We analyzed the mRNA expression of SelN in 26 different tissues of 90-day-old chickens and the expression of SelN in the muscle tissues of 12-day-old chicken embryos and 15-month-old adult chickens by quantitative real-time PCR. The results showed that SelN transcripts were expressed widely in the chicken tissues. Moreover, the expression of SelN mRNA in skeletal muscles was present at a high level in whole embryos and at a lower level in postnatal stages. However, the expression of SelN mRNA in cardiac muscle showed a different expression pattern compared with skeletal muscles. Our data indicate that the expression of the SelN gene in chicken is ubiquitous, suggesting a role of SelN in the development of chick embryo skeletal muscles.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, People's Republic of China. zjl0611@126.com
authors.forename: Jiuli
authors.initials: J
authors.lastname: Zhang
authors.forename: Jinlong
authors.initials: J
authors.lastname: Li
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Rihua
authors.initials: R
authors.lastname: Wang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Jiang
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051140:Selenoproteins / Q000235:genetics*
chemical_list: D009124:Muscle Proteins; D012333:RNA, Messenger; D051140:Selenoproteins