title: Comparison of platelet clumping and complete blood count results with Sysmex XT-2000iV in feline blood sampled on EDTA or EDTA plus CTAD (citrate, theophylline, adenosine and dipyridamole).
abstract: False thrombocytopenia may result from platelet aggregation, especially in feline ethylenediamine tetra-acetic acid (EDTA) blood specimens. Citrate, theophylline, adenosine and dipyridamole (CTAD) was added to 46 feline EDTA specimens to test its anti-aggregation action. Platelet aggregation was estimated from blood films and a complete blood count was performed with a Sysmex XT-2000iV analyser. Platelet aggregation score was >2 in 11/46 EDTA tubes and only in one EDTA+CTAD specimen. The platelet count was higher in all CTAD-supplemented tubes except one, medians measured by cytometry being 225.5 × 10(9)/l and 249.0 × 10(9)/l in EDTA and EDTA+CTAD, respectively (P = 0.007). Adding CTAD had statistically and analytically significant but moderate effects on other blood variables, the most intense variations being observed for reticulocytes (about 3% higher in EDTA specimens) and reticulocyte indexes. Addition of CTAD to EDTA when sampling feline blood is a useful option to reduce platelet clumping.
authors.affiliation: Département des Sciences Cliniques, Ecole Nationale Vétérinaire, 23 chemin des Capelles, 31 076 Toulouse Cedex 3, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Granat
authors.forename: Anne
authors.initials: A
authors.lastname: Geffré
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Braun
authors.forename: Catherine
authors.initials: C
authors.lastname: Trumel
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000662:veterinary*; D002371:Cat Diseases / Q000097:blood*; D002415:Cats / Q000097:blood; D019343:Citric Acid / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry / Q000662:veterinary; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count / Q000295:instrumentation / Q000662:veterinary; D013806:Theophylline / Q000494:pharmacology; D013921:Thrombocytopenia / Q000097:blood / Q000662:veterinary*
chemical_list: D019343:Citric Acid; D004176:Dipyridamole; D004492:Edetic Acid; D013806:Theophylline; D000241:Adenosine
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