title: [Association between polymorphisms of interleukin-6 gene promoter and breast cancer].
abstract: OBJECTIVE
  To study possible association between three single nucleotide polymorphisms (-597G/A,   -572C/G and -174G/C) of interleukin-6 (IL-6) gene promoter and breast cancer.
  
  
  METHODS
  Genomic DNA was isolated from the venous blood leukocytes from 176 unrelated patients with breast cancer and 200 healthy unrelated females (control group). Polymorphisms of -597G/A,   -572C/G and -174G/C,   were genotyped by PCR-restriction fragment length polymorphisms (PCR-RFLP). SPSS 11.5 software was employed for statistical analysis and the association of IL-6 polymorphisms with breast cancer was evaluated by x(2); test.
  
  
  RESULTS
  There was significant differences in both allele and genotype frequencies of -572C/G in case group compared with control group. The allele G of -572C/G was significantly higher in cancer patients than the controls(x(2);=15.438,   P<0.01).
  
  
  CONCLUSIONS
  There is an association between -572C/G polymorphism and breast cancer risk. The females with G allele of -572C/G are susceptible to breast cancer compared with non-carrying females.
authors.affiliation: Department of General Surgery, Hospital of Northwestern Polytechnical University, Xi'an, China. klhe270@nwpu.edu.cn
authors.forename: Kai-liang
authors.initials: KL
authors.lastname: He
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Li
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Lv
authors.forename: Dan-jie
authors.initials: DJ
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012189:Retrospective Studies
chemical_list: D015850:Interleukin-6