title: Biochemical investigation of the formation of three-dimensional networks from DNA-grafted large silica particles.
abstract: DNA is used to rationally build up networks of silica nanoparticles (SiNPs) based on the molecular recognition properties of complementary sequences. Network self-assembly is controlled from DNA covalently grafted at the surface of chemically modified SiNPs. Two strategies are compared, where grafted DNA sequences are designed in a three-strand system using noncomplementary sequences and an extra DNA linker, or in a two-strand approach for direct hybridization. In this paper, both systems are compared in terms of DNA hybridization stability, network size, and three-dimensional organization using a combination of dynamic light scattering and electron microscopy. The observed differences are discussed in terms of hybridization interactions between DNA sequences in particle-free systems through fluorescence, circular dichroism, and UV spectroscopy techniques.
authors.affiliation: UPMC-Université Paris 06, CNRS, Laboratoire de Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcelin Berthelot, F-75005 Paris.
authors.forename: Jiangyu
authors.initials: J
authors.lastname: Wu
authors.forename: Jérémie
authors.initials: J
authors.lastname: Silvent
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
authors.forename: Carole
authors.initials: C
authors.lastname: Aimé
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004586:Electrophoresis; D008854:Microscopy, Electron; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D012822:Silicon Dioxide; D004247:DNA