title: Photodynamic Antimicrobial Chemotherapy (PACT) in combination with antibiotics for treatment of Burkholderia cepacia complex infection.
abstract: This study aimed to determine if Photodynamic Antimicrobial Chemotherapy (PACT) was effective in the treatment of Burkholderia cepacia complex infection and whether a synergistic effect was evident if PACT was used in combination with antibiotics. The susceptibility of both planktonic and biofilm cultures of B. cepacia complex strains to methylene blue (MB) and meso-tetra(n-methyl-4-pyridyl)porphine tetra-tosylate (TMP)-mediated PACT was determined alone and in combination with antibiotics used in the treatment of Cystic Fibrosis pulmonary infection caused by these bacteria. When B. cepacia complex strains were grown planktonically, high levels of kill of were achieved with both TMP and MB-mediated PACT with strain and photosensitizer specific differences apparent. When strains were grown in biofilm, antibiotic treatment alone was bactericidal in 17/36 (47%) strain/antibiotic combinations tested. When antibiotic treatment was combined with PACT, bactericidal activity was apparent for 33/36 (92%) strain/antibiotic combinations. No antagonism was detected between PACT and antibiotic treatment with the combination synergistic for 6/36 (17%) and indifferent for 30/36 (83%) strain/antibiotic combinations. PACT could be a viable treatment option, either alone or in combination with antibiotics for treatment of B. cepacia complex pulmonary infection.
authors.affiliation: CF and Airways Microbiology Research Group, Queen's University Belfast, Belfast, UK.
authors.forename: Corona M
authors.initials: CM
authors.lastname: Cassidy
authors.forename: Ryan F
authors.initials: RF
authors.lastname: Donnelly
authors.forename: J Stuart
authors.initials: JS
authors.lastname: Elborn
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Magee
authors.forename: Michael M
authors.initials: MM
authors.lastname: Tunney
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects / Q000528:radiation effects; D042602:Burkholderia cepacia complex / Q000302:isolation & purification* / Q000502:physiology; D003550:Cystic Fibrosis / Q000188:drug therapy; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; C021096:tetra(4-N-methylpyridyl)porphine; D008751:Methylene Blue