title: Chronic inhibition of the respiratory chain in human fibroblast cultures: differential responses related to subject chronological and biological age.
abstract: Respiratory chain function becomes less efficient with age resulting in increased levels of damaging reactive oxygen species. We compared rotenone-exposed fibroblast strains from young and old subjects and from offspring of nonagenarian siblings and the partners of the offspring. Rotenone increased reactive oxygen species levels, inhibited growth rate, and increased telomere shortening (all p < .05). Non-stressed strains from young subjects showed lower reactive oxygen species levels (p = .031) and higher growth rates (p = .002) than strains from old subjects. Stressed strains from young subjects showed smaller increases in reactive oxygen species levels (p = .014) and larger decreases in growth rate (p < .001) than strains from old subjects. Telomere-shortening rates were not different between groups. Stress-induced decreases in growth rate were larger in strains from offspring than from partners (p = .05). Strains from young and old subjects are differentially affected by chronic inhibition of the respiratory chain. Changed growth rates in strains from offspring resemble those from strains from young subjects.
authors.affiliation: Department of Gerontology and Geriatrics, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Pim
authors.initials: P
authors.lastname: Dekker
authors.forename: Laurens M
authors.initials: LM
authors.lastname: van Baalen
authors.forename: Roeland W
authors.initials: RW
authors.lastname: Dirks
authors.forename: P Eline
authors.initials: PE
authors.lastname: Slagboom
authors.forename: Diana
authors.initials: D
authors.lastname: van Heemst
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: Rudi G J
authors.initials: RG
authors.lastname: Westendorp
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Maier
mesh_terms: D000367:Age Factors; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D001706:Biopsy; D002478:Cells, Cultured; D004579:Electron Transport / Q000187:drug effects*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D016014:Linear Models; D008297:Male; D009426:Netherlands; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000494:pharmacology*; D059506:Telomere Shortening; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D012402:Rotenone; D001616:beta-Galactosidase