title: Hepatic steatosis prevents heme oxygenase-1 induction by isoflurane in the rat liver.
abstract: OBJECTIVE
  To characterize the inductive effects of isoflurane (ISO) on hepatic heme oxygenase-1 (HO-1) in an animal model of hepatic steatosis.
  
  
  METHODS
  Lean (LEAN) and obese (FAT) Zucker rats were randomized into 4 groups: 1: LEAN + pentobarbital sodium (PEN); 2: LEAN + ISO; 3: FAT + PEN; 4: FAT + ISO. The animals were mechanically ventilated for 6 h. In vitro analyses of liver tissue included determination of HO-1 mRNA and protein expression as well as measurement of HO enzyme activity and immunohistochemical analyses.
  
  
  RESULTS
  Compared to PEN treatment, ISO administration profoundly induced hepatic HO-1 mRNA and protein expression and significantly increased HO enzyme activity in lean Zucker rats. In contrast, no difference in HO-1 gene expression was observed after ISO or PEN anesthesia in obese Zucker rats.
  
  
  CONCLUSIONS
  The present study demonstrates that ISO is an inducer of hepatic HO-1 gene expression in non-steatotic organs but failed to upregulate HO-1 in steatotic livers.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Freiburg University Medical Center, D-79106 Freiburg, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Stoll
authors.forename: Christian I
authors.initials: CI
authors.lastname: Schwer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Goebel
authors.forename: Hartmut
authors.initials: H
authors.lastname: Buerkle
authors.forename: Alexander
authors.initials: A
authors.lastname: Hoetzel
authors.forename: Rene
authors.initials: R
authors.lastname: Schmidt
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D004790:Enzyme Induction; D005234:Fatty Liver / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D005982:Glutathione Transferase / Q000097:blood; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D007530:Isoflurane / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D011924:Rats, Zucker
chemical_list: D018685:Anesthetics, Inhalation; D012333:RNA, Messenger; D007530:Isoflurane; D051547:Heme Oxygenase-1; D005982:Glutathione Transferase
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