title: Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil.
abstract: Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving.
authors.affiliation: Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. direk@tropmedres.ac
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: Premjit
authors.initials: P
authors.lastname: Amornchai
authors.forename: Gumphol
authors.initials: G
authors.lastname: Wongsuwan
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
mesh_terms: D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D016957:Burkholderia pseudomallei / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry / Q000191:economics; D012680:Sensitivity and Specificity; D012988:Soil Microbiology
chemical_list: D003470:Culture Media
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