title: A FlAsH-based cross-linker to study protein interactions in living cells.
abstract: As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).
authors.affiliation: Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Rutkowska
authors.forename: Christian H
authors.initials: CH
authors.lastname: Haering
authors.forename: Carsten
authors.initials: C
authors.lastname: Schultz
mesh_terms: D001152:Arsenicals / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D011506:Proteins / Q000737:chemistry*
chemical_list: D001152:Arsenicals; D003432:Cross-Linking Reagents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins