title: Selection of lead antibodies from naive ribosome display antibody libraries.
abstract: A large antibody fragment library (>10(12)) has been generated in ribosome display format. The library was constructed in a two-step process. First, variable (V) genes were isolated from human B cells from a panel of 14 donors and cloned into designated ribosome display vectors to create a gene bank. Second, RD-VH and RD-VL genes from individual immunoglobulin families were combined in vitro resulting in 112 scFv ribosome display sub-libraries. These were subsequently pooled to form a master library.This library was used to isolate a panel of antibodies to the IL4 receptor by three rounds of selections on a soluble target.
authors.affiliation: Department of Antibody Discovery and Protein Engineering, MedImmune Limited, Cambridge, UK. ravnp@medimmune.com
authors.forename: Peter
authors.initials: P
authors.lastname: Ravn
mesh_terms: D019020:Directed Molecular Evolution; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000276:immunology*; D019151:Peptide Library; D012270:Ribosomes / Q000235:genetics*
chemical_list: D007141:Immunoglobulin Fc Fragments; D019151:Peptide Library