title: Impact of solvent/detergent treatment of plasma on transfusion-relevant bacteria.
abstract: BACKGROUND
  A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study.
  
  
  METHODS
  A pool of apheresis plasma from four donors was spiked with eight transfusion-relevant bacteria. Plasma was treated with 1% tri(n-butyl) phosphate and 1% Triton X-45 at 31°C for 90 min and then extracted by oil at 31°C for 70 min. Decomplemented plasma and Phosphate Buffer Saline were used as controls. Bacterial count was determined in samples taken immediately after spiking, or after S/D and oil treatment. Similar experiments were conducted using three individual recovered plasma donations. Bacteria growth inhibition tests were performed using discs soaked with plasma samples whether containing the S/D agents or not.
  
  
  RESULTS
  The mean reduction factors of Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae due to complement during S/D treatment were >8·75, 4·71, and 4·18 log in pooled plasma and >7·42, 2·24 and >6·08 log in individual plasmas, respectively. Bacillus cereus and Bacillus subtilis were inactivated by S/D (>7·04 and 1·60 log in pooled, and >6·06 and 2·39 in individual plasmas, respectively). Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter cloacae did not multiply during S/D treatment of plasma. Growth inhibition tests revealed an inhibition of three gram-negative bacteria by complement and all gram-positive by S/D.
  
  
  CONCLUSIONS
  The S/D treatment of plasma does not alter the bactericidal activity of complement, and inactivates some gram-positive bacteria.
authors.affiliation: Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan.
authors.forename: M-L
authors.initials: ML
authors.lastname: Chou
authors.forename: Y-W
authors.initials: YW
authors.lastname: Wu
authors.forename: C-Y
authors.initials: CY
authors.lastname: Su
authors.forename: L-W
authors.initials: LW
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Burnouf
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001803:Blood Transfusion / Q000592:standards; D003165:Complement System Proteins; D003902:Detergents / Q000494:pharmacology*; D006090:Gram-Negative Bacteria; D006801:Humans; D010949:Plasma / Q000187:drug effects* / Q000382:microbiology; D012997:Solvents / Q000737:chemistry; D065227:Transfusion Reaction
chemical_list: D003902:Detergents; D012997:Solvents; D003165:Complement System Proteins