title: Association of six single nucleotide polymorphisms with gestational diabetes mellitus in a Chinese population.
abstract: BACKGROUND
  To investigate whether the candidate genes that confer susceptibility to type 2 diabetes mellitus are also correlated with gestational diabetes mellitus (GDM) in pregnant Chinese women.
  
  
  RESULTS
  In this study, 1764 unrelated pregnant women were recruited, of which 725 women had GDM and 1039 served as controls. Six single nucleotide polymorphisms (rs7754840 in CDKAL1, rs391300 in SRR, rs2383208 in CDKN2A/2B, rs4402960 in IGF2BP2, rs10830963 in MTNR1B, rs4607517 in GCK) were genotyped using TaqMan allelic discrimination assays. The genotype and allele distributions of each SNP between the GDM cases and controls and the combined effects of alleles for the risk of developing GDM were analyzed. We found that the rs4402960, rs2383208 and rs391300 were statistically associated with GDM (OR = 1.207, 95%CI = 1.029-1.417, p = 0.021; OR = 1.242, 95%CI = 1.077-1.432, p = 0.003; OR = 1.202, 95%CI = 1.020-1.416, P = 0.028, respectively). In addition, the effect was greater under a recessive model in rs391300 (OR = 1.820, 95%CI = 1.226-2.701, p = 0.003). Meanwhile, the joint effect of these three loci indicated an additive effect of multiple alleles on the risk of developing GDM with an OR of 1.196 per allele (p = 1.08×10(-4)). We also found that the risk alleles of rs2383208 (b = -0.085, p = 0.003), rs4402960 (b = -0.057, p = 0.046) and rs10830963 (b = -0.096, p = 0.001) were associated with HOMA-B, while rs7754840 was associated with decrease in insulin AUC during a 100 g OGTT given at the time of GDM diagnosis (b = -0.080, p = 0.007).
  
  
  CONCLUSIONS
  Several risk alleles of type 2 diabetes were associated with GDM in pregnant Chinese women. The effects of these SNPs on GDM might be through the impairment of beta cell function and these risk loci contributed additively to the disease.
authors.affiliation: Key laboratory of Endocrine, Ministry of Health, Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Nie
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Ping
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hu
authors.forename: Liangkun
authors.initials: L
authors.lastname: Ma
authors.forename: Jinsong
authors.initials: J
authors.lastname: Gao
authors.forename: Juntao
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D051360:Cyclin-Dependent Kinase 5 / Q000235:genetics; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D016640:Diabetes, Gestational / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D000081410:Germinal Center Kinases; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D019998:Racemases and Epimerases / Q000235:genetics; D012359:tRNA Methyltransferases; D000099300:Serine Racemase
chemical_list: D051360:Cyclin-Dependent Kinase 5; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D000081410:Germinal Center Kinases; D017346:Protein Serine-Threonine Kinases; D016601:RNA-Binding Proteins; D019998:Racemases and Epimerases; D012359:tRNA Methyltransferases; D000099300:Serine Racemase; C493966:CDKN2B protein, human; C498779:IGF2BP2 protein, human; C525110:CDKAL1 protein, human
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