title: Mongoliicoccus roseus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake.
abstract: Two pink, non-motile, aerobic, alkaliphilic, halotolerant, Gram-negative cocci, designated MIM28(T) and MIM29, were isolated from the surface water of a haloalkaline lake on the Mongolia Plateau. The isolates grew optimally at 30-33 °C, at pH 8-9 and with 3-4 % (w/v) NaCl. The isolates were chemoheterotrophic and could assimilate carbohydrates, organic acids and amino acids. The major respiratory quinone was menaquinone MK-7. The major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The predominant cellular fatty acids were iso-C(15 : 0) (13.8-17.5 %), anteiso-C(15 : 0) (10.5-11.2 %), iso-C(16 : 0) (9.9-13.0 %), C(16 : 0) (4.3-4.6 %), iso-C(17 : 0) (3.8-5.3 %), anteiso-C(17 : 0) (3.7-7.1 %), C(17 : 1)ω6c (4.6-6.4 %), iso-C(17 : 0) 3-OH (4.6-5.8 %), summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c; 4.0-6.4 %) and summed feature 9 (iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl; 10.4-12.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were most closely related to Litoribacter ruber YIM CH208(T) (93.6 % 16S rRNA gene sequence similarity), the genus Echinicola (90.4-92 %) and other members of the family Cyclobacteriaceae (87.8-90 %). The DNA G+C contents of strains MIM28(T) and MIM29 were 62.8 and 62.2 mol%. On the basis of morphology, physiology, fatty acid composition, phylogeny and 16S rRNA gene sequence analysis, the isolates are assigned to a novel species of a new genus, for which we propose the name Mongoliicoccus roseus gen. nov., sp. nov.; the type strain of the type species is MIM28(T) (= ACCC 05511(T) = KCTC 19808(T)).
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.
authors.forename: Ya Peng
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, PR China.
authors.forename: Yong Xia
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.
authors.forename: Yun Xu
authors.initials: YX
authors.lastname: Li
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.
authors.forename: Fu Ying
authors.initials: FY
authors.lastname: Feng
authors.affiliation: Institution for Applied and Environmental Microbiology, Inner Mongolia Agriculture University, Huhhot 010018, PR China.
authors.forename: Hui Rong
authors.initials: HR
authors.lastname: Liu
authors.affiliation: Department of Preventive Medicine Public Health School, Inner Mongolia Medical College, Huhhot 010110, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D008986:Mongolia; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S