title: Anti-inflammatory effect with high intensity focused ultrasound-mediated pulsatile delivery of diclofenac.
abstract: A pulsatile ultrasound controlled drug release platform with diclofenac-loaded alginate microcapsules (fabricated with a home-made electrostatic device, 75% embedded rate) was established to evaluate anti-inflammation efficiency. Better anti-inflammation efficiency was found using the ultrasound system and the drug delivery can be adjusted based on the programmed ultrasound cycle. The results of the in vitro study show that an approx. 30% higher drug release rate was obtained by using continuous ultrasound irradiation (9-Watt, 180 min), and an approx. 16% higher drug release rate was obtained by using pulsatile ultrasound irradiation (9-Watt, 60 min) compared to without ultrasound activation. For the in vivo study, the anti-inflammatory test with carrageenan-induced rat's paw edema shows that diclofenac-loaded microcapsules followed by ultrasound irradiation (9-Watt, 60 min) contributed to an 81% inhibition rate, which was significantly higher than diclofenac only (approx. 60% higher). In addition, because of their heat conducting properties, gold nanoparticles encapsulated in the diclofenac-loaded microcapsules resulted in better drug release efficiency, but tended to depress the anti-inflammation effect.
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Taiwan, ROC.
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Chih-Hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Yung-Sheng
authors.initials: YS
authors.lastname: Lin
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Chen
authors.forename: Keng-Shiang
authors.initials: KS
authors.lastname: Huang
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D002214:Capsules; D002351:Carrageenan; D003692:Delayed-Action Preparations; D004008:Diclofenac / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000494:pharmacology* / Q000627:therapeutic use; D004487:Edema / Q000188:drug therapy; D020723:Glucuronic Acid / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019220:High-Energy Shock Waves; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000464:Alginates; D000893:Anti-Inflammatory Agents; D002214:Capsules; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D004008:Diclofenac; D006046:Gold; D020723:Glucuronic Acid; D002351:Carrageenan