title: Human cumulus cells molecular signature in relation to oocyte nuclear maturity stage.
abstract: The bi-directional communication between the oocyte and the surrounding cumulus cells (CCs) is crucial for the acquisition of oocyte competence. We investigated the transcriptomic profile of human CCs isolated from mature and immature oocytes under stimulated cycle. We used human Genome U133 Plus 2.0 microarrays to perform an extensive analysis of the genes expressed in human CCs obtained from patients undergoing intra-cytoplasmic sperm injection. CC samples were isolated from oocyte at germinal vesicle, stage metaphase I and stage metaphase II. For microarray analysis, we used eight chips for each CC category. Significance analysis of microarray multiclass was used to analyze the microarray data. Validation was performed by RT-qPCR using an independent cohort of CC samples. We identified differentially over-expressed genes between the three CC categories. This study revealed a specific signature of gene expression in CCs issued from MII oocyte compared with germinal vesicle and metaphase I. The CC gene expression profile, which is specific of MII mature oocyte, can be useful as predictors of oocyte quality.
authors.affiliation: CHU Montpellier, Institute for Research in Biotherapy, Hôpital Saint-Eloi, INSERM U1040, Montpellier, France.
authors.forename: Zamalou Gisèle
authors.initials: ZG
authors.lastname: Ouandaogo
authors.forename: Delphine
authors.initials: D
authors.lastname: Haouzi
authors.forename: Said
authors.initials: S
authors.lastname: Assou
authors.forename: Hervé
authors.initials: H
authors.lastname: Dechaud
authors.forename: Issac Jacques
authors.initials: IJ
authors.lastname: Kadoch
authors.forename: John
authors.initials: J
authors.lastname: De Vos
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D002450:Cell Communication; D002453:Cell Cycle; D002467:Cell Nucleus / Q000502:physiology*; D015331:Cohort Studies; D054885:Cumulus Cells / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D009865:Oocytes / Q000166:cytology*; D020554:Sperm Injections, Intracytoplasmic
references.citation: Biol Reprod. 2009 May;80(5):1015-25
references.pmid: 19164180
references.citation: J Assist Reprod Genet. 2011 Jan;28(1):31-40
references.pmid: 20859762
references.citation: Mol Hum Reprod. 2010 Apr;16(4):229-40
references.pmid: 19933312
references.citation: Endocrinology. 2005 Jan;146(1):309-17
references.pmid: 15459113
references.citation: Hum Reprod. 2010 May;25(5):1259-70
references.pmid: 20228394
references.citation: Mol Hum Reprod. 1999 Oct;5(10):920-6
references.pmid: 10508219
references.citation: Hum Reprod. 2005 Jun;20(6):1485-92
references.pmid: 15774546
references.citation: Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8
references.pmid: 9843981
references.citation: Endocrinology. 2010 Mar;151(3):1247-56
references.pmid: 20032053
references.citation: Biol Reprod. 2006 Dec;75(6):868-76
references.pmid: 16943366
references.citation: Mol Hum Reprod. 2010 Aug;16(8):531-8
references.pmid: 20435608
references.citation: Fertil Steril. 2005 Apr;83 Suppl 1:1169-79
references.pmid: 15831290
references.citation: Hum Reprod. 2011 Jun;26(6):1440-9
references.pmid: 21427117
references.citation: Hum Reprod Update. 1998 Mar-Apr;4(2):103-20
references.pmid: 9683349
references.citation: Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10501-6
references.pmid: 10468638
references.citation: Reprod Biol. 2009 Nov;9(3):203-24
references.pmid: 19997475
references.citation: Mol Hum Reprod. 2009 Feb;15(2):89-103
references.pmid: 19141487
references.citation: Hum Reprod. 2009 Nov;24(11):2868-78
references.pmid: 19625307
references.citation: Hum Reprod. 2008 May;23(5):1118-27
references.pmid: 18310048
references.citation: BMC Genomics. 2011 Jan 24;12:57
references.pmid: 21261964
references.citation: J Biol Chem. 1989 Aug 15;264(23):13840-7
references.pmid: 2503506
references.citation: Mol Hum Reprod. 2008 Dec;14(12):711-9
references.pmid: 19028806
references.citation: Biol Reprod. 2003 Mar;68(3):804-11
references.pmid: 12604629
references.citation: Theriogenology. 2011 Jan 15;75(2):362-76
references.pmid: 20965553
references.citation: Hum Reprod. 2007 Dec;22(12):3069-77
references.pmid: 17951581
references.citation: Hum Reprod. 1998 Jun;13(6):1638-44
references.pmid: 9688405
references.citation: Endocrinology. 2008 Jun;149(6):3187-95
references.pmid: 18325989
references.citation: Mol Hum Reprod. 2008 Mar;14(3):157-68
references.pmid: 18204071
references.citation: Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21
references.pmid: 11309499
references.citation: Hum Reprod. 2006 Jul;21(7):1705-19
references.pmid: 16571642
references.citation: Hum Reprod. 2004 Dec;19(12):2869-74
references.pmid: 15471935
references.citation: Biochimie. 2010 Jun;92(6):665-75
references.pmid: 20399827