title: Rhythmic release of prothoracicotropic hormone from the brain of an adult insect during egg development.
abstract: Prothoracicotropic hormone (PTTH) is a brain neurohormone that has been studied for over 80 years. The only known target of PTTH is the prothoracic glands (PGs) of larvae, which synthesize the insect molting hormones (ecdysteroids) and a massive literature exists on this axis. The PGs degenerate around the time of adult emergence, yet presence of PTTH has been reported in the brains of several adult insects. Using an in vitro bioassay system, we confirm that PTTH is present in the adult female brain of Rhodnius prolixus. The material is electrophoretically, immunologically and biologically indistinguishable from larval PTTH. The amount of PTTH in the brain shows a daily rhythm during egg development. We show that brains in vitro release PTTH with a daily rhythm over this period of time. PTTH is released at each scotophase. This is the first report that PTTH is released from the adult brain and functions as a hormone, inviting explanation of its function. Larval PTTH is also known to be released with a daily rhythm, and the clock in the brain controls both larval and adult rhythms. The potential significance of rhythmic PTTH release in female adults is discussed in relation to the regulation of ecdysteroids, egg development and the concept of internal temporal order.
authors.affiliation: Department of Biology, York University, 4700 Keele St., Toronto, ON, Canada, M3J 1P3. xanthev@yorku.ca
authors.forename: Xanthe
authors.initials: X
authors.lastname: Vafopoulou
authors.forename: Michael
authors.initials: M
authors.lastname: Cardinal-Aucoin
authors.forename: Colin G H
authors.initials: CG
authors.lastname: Steel
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology*; D026461:Ecdysteroids / Q000096:biosynthesis; D005260:Female; D047468:Immunoprecipitation; D007301:Insect Hormones / Q000378:metabolism*; D009929:Organ Size; D010053:Ovary / Q000033:anatomy & histology; D010063:Ovum / Q000254:growth & development* / Q000378:metabolism; D012239:Rhodnius / Q000254:growth & development* / Q000378:metabolism*; D014020:Tissue Extracts
chemical_list: D026461:Ecdysteroids; D007301:Insect Hormones; D014020:Tissue Extracts; C009089:prothoracicotropic hormone