title: Dietary CLA combined with palm oil or ovine fat differentially influences fatty acid deposition in tissues of obese Zucker rats.
abstract: The effect of dietary conjugated linoleic acid (CLA) supplementation in combination with fat from vegetable versus animal origin on the fatty acid deposition, including that of individual 18:1 and 18:2 (conjugated and non-conjugated) isomers, in the liver and muscle of obese rats was investigated. For this purpose, 32 male Zucker rats were randomly assigned to one of four diets containing palm oil or ovine fat, supplemented or not with 1% of 1:1 cis(c)9,trans(t)11 and t10,c12 CLA isomers mixture. Total fatty acid content decreased in the liver and muscle of CLA-fed rats. In the liver, CLA increased saturated fatty acids (SFA) in 11.9% and decreased monounsaturated fatty acids (MUFA) in 6.5%. n-3 Polyunsaturated fatty acids (PUFA) relative proportions were increased in 30.6% by CLA when supplemented to the ovine fat diet. In the muscle, CLA did not affect SFA but decreased MUFA and PUFA percentages. The estimation of Δ9-indices 16 and 18 suggested that CLA inhibited the stearoyl-CoA desaturase activity in the liver (a decrease of 13-38%), in particular when supplemented to the ovine fat diet. Concerning CLA supplementation, the t10,c12 isomer percentage was 60-80% higher in the muscle than in the liver. It is of relevance that rats fed ovine fat, containing bio-formed CLA, had more c9,t11 CLA isomer deposited in both tissues than rats fed palm oil plus synthetic CLA. These results highlight the importance to further clarify the biological effects of consuming foods naturally enriched in CLA, alternatively to CLA dietary supplementation.
authors.affiliation: Secção de Bioquímica, CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisbon, Portugal.
authors.forename: Susana V
authors.initials: SV
authors.lastname: Martins
authors.forename: Paula A
authors.initials: PA
authors.lastname: Lopes
authors.forename: Susana P
authors.initials: SP
authors.lastname: Alves
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Alfaia
authors.forename: Matilde F
authors.initials: MF
authors.lastname: Castro
authors.forename: Rui J B
authors.initials: RJ
authors.lastname: Bessa
authors.forename: José A M
authors.initials: JA
authors.lastname: Prates
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D001823:Body Composition / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D044243:Linoleic Acids, Conjugated / Q000378:metabolism / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011924:Rats, Zucker; D034561:Sheep, Domestic; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D044243:Linoleic Acids, Conjugated; D010938:Plant Oils; C503589:cis-9, trans-11-conjugated linoleic acid; C496197:trans-10,cis-12-conjugated linoleic acid; D000073878:Palm Oil; D013230:Stearoyl-CoA Desaturase
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