title: Development of a multilayered polymeric DNA biosensor using radio frequency technology with gold and magnetic nanoparticles.
abstract: This study utilized the radio frequency (RF) technology to develop a multilayered polymeric DNA sensor with the help of gold and magnetic nanoparticles. The flexible polymeric materials, poly (p-xylylene) (Parylene) and polyethylene naphtholate (PEN), were used as substrates to replace the conventional rigid substrates such as glass and silicon wafers. The multilayered polymeric RF biosensor, including the two polymer layers and two copper transmission structure layers, was developed to reduce the total sensor size and further enhance the sensitivity of the biochip in the RF DNA detection. Thioglycolic acid (TGA) was used on the surface of the proposed biochip to form a thiolate-modified sensing surface for DNA hybridization. Gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) were used to immobilize on the surface of the biosensor to enhance overall detection sensitivity. In addition to gold nanoparticles, the magnetic nanoparticles has been demonstrated the applicability for RF DNA detection. The performance of the proposed biosensor was evaluated by the shift of the center frequency of the RF biosensor because the electromagnetic characteristic of the biosensors can be altered by the immobilized multilayer nanoparticles on the biosensor. The experimental results show that the detection limit of the DNA concentration can reach as low as 10 pM, and the largest shift of the center frequency with triple-layer AuNPs and MNPs can approach 0.9 and 0.7 GHz, respectively. Such the achievement implies that the developed biosensor can offer an alternative inexpensive, disposable, and highly sensitive option for application in biomedicine diagnostic systems because the price and size of each biochip can be effectively reduced by using fully polymeric materials and multilayer-detecting structures.
authors.affiliation: Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Cheng-Hao
authors.initials: CH
authors.lastname: Yang
authors.forename: Long-Sheng
authors.initials: LS
authors.lastname: Kuo
authors.forename: Ping-Hei
authors.initials: PH
authors.lastname: Chen
authors.forename: Chii-Rong
authors.initials: CR
authors.lastname: Yang
authors.forename: Zuo-Min
authors.initials: ZM
authors.lastname: Tsai
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation; D011108:Polymers / Q000737:chemistry; D011846:Radio Waves; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D058185:Magnetite Nanoparticles; D011108:Polymers; D006046:Gold; D004247:DNA