title: Mapping local electric fields in proteins at biomimetic interfaces.
abstract: We present a novel approach for determining the strength of the electric field experienced by proteins immobilised on membrane models. It is based on the vibrational Stark effect of a nitrile label introduced at different positions on engineered proteins and monitored by surface enhanced infrared absorption spectroscopy.
authors.affiliation: Technische Universität Berlin, Institut für Chemie, Sekr. PC14, Strasse des 17. Juni 135, D-10623 Berlin, Germany.
authors.forename: Gal
authors.initials: G
authors.lastname: Schkolnik
authors.forename: Tillmann
authors.initials: T
authors.lastname: Utesch
authors.forename: Johannes
authors.initials: J
authors.lastname: Salewski
authors.forename: Katalin
authors.initials: K
authors.lastname: Tenger
authors.forename: Diego
authors.initials: D
authors.lastname: Millo
authors.forename: Anja
authors.initials: A
authors.lastname: Kranich
authors.forename: Ingo
authors.initials: I
authors.lastname: Zebger
authors.forename: Claudia
authors.initials: C
authors.lastname: Schulz
authors.forename: László
authors.initials: L
authors.lastname: Zimányi
authors.forename: Gábor
authors.initials: G
authors.lastname: Rákhely
authors.forename: Maria Andrea
authors.initials: MA
authors.lastname: Mroginski
authors.forename: Peter
authors.initials: P
authors.lastname: Hildebrandt
mesh_terms: D032701:Biomimetics / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D004560:Electricity; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D014732:Vibration
chemical_list: D055767:Immobilized Proteins; D045304:Cytochromes c