title: Synthesis and evaluation of novel analogues of mangiferin as potent antipyretic.
abstract: OBJECTIVE
  To screen different analogues of mangiferin pharmacologically for antipyretic activity.
  
  
  METHODS
  The naturally occurring xanthone glycoside mangiferin was isolated by column chromatography from the ethanolic extract of stem bark of Mangifera indica. Mangiferin was further converted to 5-(N-phenylamino methyleno) mangiferin, 5-(N-p-chlorophenylamino methyleno) mangiferin, 5-(N-2-methyl phenylamino methyleno) mangiferin, 5-(N-p-methoxy phenylamino methyleno) mangiferin, 5-(N, N-diphenylamino methyleno) mangiferin, 5-(N-α-napthylamino methyleno) mangiferin and 5-(N-4-methyl phenylamino methyleno) mangiferin analogues. The synthesized compounds were further screened for antipyretic activity along with mangiferin at a dose level of 100 and 200 mg/kg. Mangiferin and its analogues were characterized by melting point andR(f)value determination and through spectral technique like UV, IR, and NMR spectral analysis.
  
  
  RESULTS
  The antipyretic activity of mangiferin as well as all analogues was found to be more significant in at higher dose ie. 200 mg/kg which was depicted through a decrease in rectal temperature up to 3 h.
  
  
  CONCLUSIONS
  The antipyretic activity of mangiferin and its analogues may be attributed to inhibition in synthesis of TNF-α and anti-oxidant activity associated with amelioration of inflammatory actions of cytokines.
authors.affiliation: Varanasi College of Pharmacy, Uttar Pradesh, Varanasi, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Kant Singh
authors.forename: Saurabh K
authors.initials: SK
authors.lastname: Sinha
authors.forename: S K
authors.initials: SK
authors.lastname: Prasad
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: Bhawani S
authors.initials: BS
authors.lastname: Bithu
authors.forename: S
authors.initials: S
authors.lastname: Sadish Kumar
authors.forename: P
authors.initials: P
authors.lastname: Singh
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D006027:Glycosides / Q000494:pharmacology / Q000627:therapeutic use; D031022:Mangifera / Q000737:chemistry*; D051379:Mice; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012441:Saccharomyces cerevisiae; D016896:Treatment Outcome; D044004:Xanthones / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin