title: Sex differences and synchronous development of steroid receptor coactivator-1 and synaptic proteins in the hippocampus of postnatal female and male C57BL/6 mice.
abstract: The structure and function including synaptic plasticity of the hippocampus are deeply affected by steroids in a sex-dependant manner, these processes are believed to be mediated by steroid receptors though their coactivators. Our previous studies have reported the developmental profiles of steroid receptor coactivator-1 (SRC-1) and PSD-95 in the hippocampus of postnatal female rats and the sex-differences of SRC-1 immunoreactivities in the brain of adult mice. However, whether there are any sex differences about postnatal development of SRC-1 and synaptic proteins in the hippocampus remain unclear. In this study, we investigated the postnatal profile of SRC-1 and key synaptic protein synaptophysin (SYN), PSD-95 and GluR1 in the hippocampus of female and male mice using immunohistochemistry and Western blot. The results showed that in the female hippocampus, the highest levels of SRC-1 were detected at P14, SYN and GluR1 at P30 and PSD-95 at P60; while in the males, the highest levels of SRC-1, SYN and GluR1 were detected at P30, and PSD-95 at P60. Female hippocampus tended to have higher levels of SRC-1, SYN and GluR1 before P30 and PSD-95 before P14; while male hippocampus have higher levels of PSD-95 at P14, P60 and GluR1 at P0. Correlation analysis showed the profiles of SRC-1 were highly correlated with each synaptic protein. The above results showed that in the hippocampus, except some minor sex differences detected at some time-point examined, females and males shared similar postnatal developmental profile and SRC-1 may be deeply involved in the regulation of hippocampal synaptogenesis.
authors.affiliation: Department of Neurobiology, Chongqing Key Laboratory of Neurobiology, Third Military Medical University, Chongqing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Bian
authors.forename: Kongjiang
authors.initials: K
authors.lastname: Zhu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Wenqin
authors.initials: W
authors.lastname: Cai
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D000076122:Disks Large Homolog 4 Protein; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051528:Guanylate Kinases / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics / Q000378:metabolism*; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission; D016708:Synaptophysin; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000076122:Disks Large Homolog 4 Protein; D051528:Guanylate Kinases; D008565:Membrane Proteins; D056920:Nuclear Receptor Coactivator 1; D018091:Receptors, AMPA; D011987:Receptors, Steroid; D016708:Synaptophysin; D033921:Vesicular Transport Proteins; C501447:Dlg4 protein, mouse; C569015:Syp protein, mouse; C538861:Ncoa1 protein, mouse