title: Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011.
abstract: In October 2010, the US Centers for Disease Control and Prevention received reports of cases of severe watery diarrhea in Haiti. The cause was confirmed to be toxigenic Vibrio cholerae, serogroup O1, serotype Ogawa, biotype El Tor. We characterized 122 isolates from Haiti and compared them with isolates from other countries. Antimicrobial drug susceptibility was tested by disk diffusion and broth microdilution. Analyses included identification of rstR and VC2346 genes, sequencing of ctxAB and tcpA genes, and pulsed-field gel electrophoresis with SfiI and NotI enzymes. All isolates were susceptible to doxycycline and azithromycin. One pulsed-field gel electrophoresis pattern predominated, and ctxB sequence of all isolates matched the B-7 allele. We identified the tcpETCIRS allele, which is also present in Bangladesh strain CIRS 101. These data show that the isolates from Haiti are clonally and genetically similar to isolates originating in Africa and southern Asia and that ctxB-7 and tcpET(CIRS) alleles are undergoing global dissemination.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. dft1@cdc.gov
authors.forename: Deborah
authors.initials: D
authors.lastname: Talkington
authors.forename: Cheryl
authors.initials: C
authors.lastname: Bopp
authors.forename: Cheryl
authors.initials: C
authors.lastname: Tarr
authors.forename: Michele B
authors.initials: MB
authors.lastname: Parsons
authors.forename: Georges
authors.initials: G
authors.lastname: Dahourou
authors.forename: Molly
authors.initials: M
authors.lastname: Freeman
authors.forename: Kevin
authors.initials: K
authors.lastname: Joyce
authors.forename: Maryann
authors.initials: M
authors.lastname: Turnsek
authors.forename: Nancy
authors.initials: N
authors.lastname: Garrett
authors.forename: Michael
authors.initials: M
authors.lastname: Humphrys
authors.forename: Gerardo
authors.initials: G
authors.lastname: Gomez
authors.forename: Steven
authors.initials: S
authors.lastname: Stroika
authors.forename: Jacques
authors.initials: J
authors.lastname: Boncy
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ochieng
authors.forename: Joseph
authors.initials: J
authors.lastname: Oundo
authors.forename: John
authors.initials: J
authors.lastname: Klena
authors.forename: Anthony
authors.initials: A
authors.lastname: Smith
authors.forename: Karen
authors.initials: K
authors.lastname: Keddy
authors.forename: Peter
authors.initials: P
authors.lastname: Gerner-Smidt
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques; D002771:Cholera / Q000453:epidemiology; D002772:Cholera Toxin / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006205:Haiti / Q000453:epidemiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors; D002772:Cholera Toxin
references.citation: Infect Immun. 1986 Nov;54(2):415-20
references.pmid: 3021628
references.citation: J Infect. 2008 Sep;57(3):280-1
references.pmid: 18657323
references.citation: OMICS. 2010 Feb;14(1):1-7
references.pmid: 20141327
references.citation: Environ Microbiol. 2006 Mar;8(3):526-634
references.pmid: 16478458
references.citation: J Clin Microbiol. 1992 Aug;30(8):2118-21
references.pmid: 1500520
references.citation: Emerg Infect Dis. 2011 Nov;17(11):2151-4
references.pmid: 22099122
references.citation: Trends Microbiol. 2010 Jan;18(1):46-54
references.pmid: 19942436
references.citation: J Clin Microbiol. 2000 Nov;38(11):4145-51
references.pmid: 11060082
references.citation: MMWR Morb Mortal Wkly Rep. 2010 Nov 19;59(45):1473-9
references.pmid: 21085088
references.citation: Int J Hyg Environ Health. 2006 Nov;209(6):521-6
references.pmid: 16891156
references.citation: J Appl Microbiol. 1997 Dec;83(6):727-36
references.pmid: 9449811
references.citation: Foodborne Pathog Dis. 2006 Spring;3(1):51-8
references.pmid: 16602979
references.citation: N Engl J Med. 2011 Jan 6;364(1):33-42
references.pmid: 21142692
references.citation: Int J Infect Dis. 2010 May;14(5):e384-9
references.pmid: 19781971
references.citation: Microbiol Mol Biol Rev. 1998 Dec;62(4):1301-14
references.pmid: 9841673
references.citation: Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3129-34
references.pmid: 11854465
references.citation: J Bacteriol. 2010 Jul;192(13):3524-33
references.pmid: 20348258
references.citation: Microbiology (Reading). 2002 Jun;148(Pt 6):1655-1666
references.pmid: 12055286
references.citation: Infect Immun. 2002 May;70(5):2441-53
references.pmid: 11953381
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Emerg Infect Dis. 2011 Nov;17(11):2113-21
references.pmid: 22099115
references.citation: Nature. 2000 Aug 3;406(6795):477-83
references.pmid: 10952301
references.citation: Lancet. 1993 Jun 26;341(8861):1661
references.pmid: 8100020
references.citation: Microb Pathog. 1997 Apr;22(4):199-208
references.pmid: 9140915
references.citation: Jpn J Infect Dis. 2006 Aug;59(4):266-9
references.pmid: 16936349
references.citation: J Clin Microbiol. 1993 Jan;31(1):22-5
references.pmid: 7678018