title: Engineered biological entities for drug delivery and gene therapy protein nanoparticles.
abstract: The development of genetic engineering techniques has speeded up the growth of the biotechnological industry, resulting in a significant increase in the number of recombinant protein products on the market. The deep knowledge of protein function, structure, biological interactions, and the possibility to design new polypeptides with desired biological activities have been the main factors involved in the increase of intensive research and preclinical and clinical approaches. Consequently, new biological entities with added value for innovative medicines such as increased stability, improved targeting, and reduced toxicity, among others have been obtained. Proteins are complex nanoparticles with sizes ranging from a few nanometers to a few hundred nanometers when complex supramolecular interactions occur, as for example, in viral capsids. However, even though protein production is a delicate process that imposes the use of sophisticated analytical methods and negative secondary effects have been detected in some cases as immune and inflammatory reactions, the great potential of biodegradable and tunable protein nanoparticles indicates that protein-based biotechnological products are expected to increase in the years to come.
authors.affiliation: Institute for Biotechnology and Biomedicine, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Domingo-Espín
authors.forename: Ugutz
authors.initials: U
authors.lastname: Unzueta
authors.forename: Paolo
authors.initials: P
authors.lastname: Saccardo
authors.forename: Escarlata
authors.initials: E
authors.lastname: Rodríguez-Carmona
authors.forename: José Luís
authors.initials: JL
authors.lastname: Corchero
authors.forename: Esther
authors.initials: E
authors.lastname: Vázquez
authors.forename: Neus
authors.initials: N
authors.lastname: Ferrer-Miralles
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020811:Biomedical Technology; D016503:Drug Delivery Systems / Q000379:methods*; D015316:Genetic Therapy; D006801:Humans; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000627:therapeutic use*; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000627:therapeutic use*
chemical_list: D011506:Proteins
references.citation: Annu Rev Biomed Eng. 2008;10:169-94
references.pmid: 18647114
references.citation: Hepatology. 2002 Nov;36(5):1106-14
references.pmid: 12395320
references.citation: Mol Cell Biol. 1988 Oct;8(10):4048-54
references.pmid: 3054508
references.citation: Appl Microbiol Biotechnol. 2007 Nov;77(1):13-22
references.pmid: 17704915
references.citation: Mol Ther. 2003 Jul;8(1):143-50
references.pmid: 12842437
references.citation: Vaccine. 2008 Mar 28;26(15):1846-54
references.pmid: 18325641
references.citation: Mol Cell Neurosci. 2004 Oct;27(2):85-131
references.pmid: 15485768
references.citation: FEBS Lett. 2002 Jul 3;522(1-3):93-8
references.pmid: 12095625
references.citation: Trends Microbiol. 2010 Sep;18(9):423-30
references.pmid: 20674365
references.citation: AAPS J. 2007 Feb 09;9(1):E18-29
references.pmid: 17408236
references.citation: Curr Protein Pept Sci. 2002 Aug;3(4):399-407
references.pmid: 12370003
references.citation: FEBS Lett. 2004 Jan 30;558(1-3):63-8
references.pmid: 14759517
references.citation: J Neurosci. 2002 Jul 1;22(13):5423-31
references.pmid: 12097494
references.citation: J Biomol Screen. 2011 Jan;16(1):1-14
references.pmid: 21160066
references.citation: Biochim Biophys Acta. 2008 Dec;1786(2):126-38
references.pmid: 18440319
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15739-46
references.pmid: 17023540
references.citation: J Bacteriol. 1993 May;175(10):2871-9
references.pmid: 8491708
references.citation: FEBS Lett. 2003 Jun 5;544(1-3):262-7
references.pmid: 12782328
references.citation: Vaccine. 2008 Aug 18;26(35):4594-601
references.pmid: 18588935
references.citation: Curr Opin Biotechnol. 2007 Dec;18(6):537-45
references.pmid: 18083549
references.citation: Biologicals. 1994 Jun;22(2):127-33
references.pmid: 7917229
references.citation: Int J Cancer. 2007 Dec 15;121(12):2794-800
references.pmid: 17721997
references.citation: Protein Eng Des Sel. 2011 Jan;24(1-2):53-63
references.pmid: 20923881
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4150-5
references.pmid: 18334651
references.citation: Drug Discov Today. 2008 Dec;13(23-24):1067-74
references.pmid: 18801457
references.citation: Annu Rev Biophys Biomol Struct. 2000;29:105-53
references.pmid: 10940245
references.citation: Cancer Res. 2006 Apr 1;66(7):3764-72
references.pmid: 16585203
references.citation: Drug Discov Today. 2007 Jan;12(1-2):54-61
references.pmid: 17198973
references.citation: Appl Microbiol Biotechnol. 2003 Jul;62(1):1-20
references.pmid: 12733003
references.citation: Curr Opin Biotechnol. 1994 Feb;5(2):175-9
references.pmid: 7764796
references.citation: Biochemistry. 1990 Sep 18;29(37):8713-9
references.pmid: 2271551
references.citation: Biomacromolecules. 2009 Dec 14;10(12):3199-206
references.pmid: 19874026
references.citation: J Virol. 1999 May;73(5):4465-9
references.pmid: 10196348
references.citation: Biochem J. 2001 Jun 15;356(Pt 3):867-73
references.pmid: 11389696
references.citation: J Biomed Sci. 2009 Aug 11;16:69
references.pmid: 19671144
references.citation: PLoS One. 2008;3(10):e3570
references.pmid: 18974784
references.citation: Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7934-8
references.pmid: 1518816
references.citation: Biotechnol Bioeng. 2009 Feb 15;102(3):845-55
references.pmid: 18958863
references.citation: Nucleic Acids Res. 1983 Mar 11;11(5):1295-308
references.pmid: 6298741
references.citation: Adv Biochem Eng Biotechnol. 2002;74:183-206
references.pmid: 11991179
references.citation: Eur J Cell Biol. 2001 Nov;80(11):669-77
references.pmid: 11824786
references.citation: Mol Ther. 2002 Apr;5(4):447-54
references.pmid: 11945072
references.citation: Biochemistry. 2006 Jan 31;45(4):1116-27
references.pmid: 16430208
references.citation: Chem Soc Rev. 2006 Nov;35(11):1105-10
references.pmid: 17057839
references.citation: FASEB J. 2007 Sep;21(11):2664-71
references.pmid: 17463227
references.citation: Trends Biotechnol. 2008 May;26(5):267-75
references.pmid: 18358551
references.citation: Immunol Cell Biol. 2002 Apr;80(2):119-30
references.pmid: 11940112
references.citation: J Bacteriol. 1970 Feb;101(2):618-27
references.pmid: 5413830
references.citation: EMBO J. 1994 Sep 1;13(17):4126-36
references.pmid: 8076608
references.citation: Drug Discov Today. 2008 Oct;13(19-20):882-7
references.pmid: 18656548
references.citation: J Biol Chem. 2003 Mar 28;278(13):11411-8
references.pmid: 12519756
references.citation: Viral Immunol. 2007 Sep;20(3):453-60
references.pmid: 17931115
references.citation: J Mol Recognit. 2003 Sep-Oct;16(5):265-71
references.pmid: 14523939
references.citation: Virology. 2009 Feb 20;384(2):389-99
references.pmid: 19157478
references.citation: Trends Biotechnol. 2002 Apr;20(4):173-80
references.pmid: 11906750
references.citation: J Bacteriol. 2004 Feb;186(3):623-30
references.pmid: 14729686
references.citation: J Neurosci Methods. 2003 May 30;125(1-2):113-20
references.pmid: 12763237
references.citation: Biol Chem. 1999 Mar;380(3):381-6
references.pmid: 10223341
references.citation: Cancer Res. 2005 Sep 1;65(17):7840-6
references.pmid: 16140953
references.citation: J Gene Med. 2004 Nov;6(11):1247-56
references.pmid: 15368587
references.citation: Nat Med. 2000 Dec;6(12):1362-7
references.pmid: 11100121
references.citation: Adv Drug Deliv Rev. 2004 Apr 23;56(7):967-85
references.pmid: 15066755
references.citation: Cancer Res. 2004 Apr 15;64(8):2853-7
references.pmid: 15087403
references.citation: J Biomed Biotechnol. 2010;2010:317545
references.pmid: 20414353
references.citation: J Virol Methods. 2000 Oct;90(1):85-90
references.pmid: 11011084
references.citation: Hum Gene Ther. 2005 Sep;16(9):1110-5
references.pmid: 16149909
references.citation: J Gene Med. 2009 Nov;11(11):1020-9
references.pmid: 19757487
references.citation: Cancer Gene Ther. 2003 Jul;10(7):529-39
references.pmid: 12833133
references.citation: J Bacteriol. 2003 Sep;185(17):5086-95
references.pmid: 12923081
references.citation: Clin Cancer Res. 2005 Sep 1;11(17):6317-24
references.pmid: 16144936
references.citation: J Biol Chem. 2002 Sep 6;277(36):32480-9
references.pmid: 12080050
references.citation: Biochem Pharmacol. 2006 Feb 14;71(4):416-25
references.pmid: 16376307
references.citation: FEBS Lett. 1998 Jun 16;429(3):269-73
references.pmid: 9662430
references.citation: Gene Ther. 1998 Apr;5(4):521-30
references.pmid: 9614577
references.citation: J Pharmacol Exp Ther. 1991 Oct;259(1):66-70
references.pmid: 1920136
references.citation: J Drug Target. 2003 Jul;11(6):333-43
references.pmid: 14668054
references.citation: Biochem Pharmacol. 2007 Mar 1;73(5):620-31
references.pmid: 17161827
references.citation: Biochemistry. 1997 Mar 11;36(10):3008-17
references.pmid: 9062132
references.citation: J Mol Biol. 2006 Sep 29;362(4):753-70
references.pmid: 16935304
references.citation: Gene Ther. 1997 Sep;4(9):961-8
references.pmid: 9349433
references.citation: Arch Virol. 2006 Nov;151(11):2133-48
references.pmid: 16791442
references.citation: Gene Ther. 2004 Oct;11 Suppl 1:S10-7
references.pmid: 15454952
references.citation: Curr Drug Deliv. 2004 Apr;1(2):119-27
references.pmid: 16305377
references.citation: Curr Gene Ther. 2006 Aug;6(4):459-80
references.pmid: 16918335
references.citation: Eur J Cancer. 2007 May;43(8):1242-50
references.pmid: 17449238
references.citation: Nat Biotechnol. 2001 Mar;19(3):225-30
references.pmid: 11231554
references.citation: FASEB J. 2000 Oct;14(13):2108-18
references.pmid: 11023995
references.citation: Gene Ther. 2000 Dec;7(24):2122-31
references.pmid: 11223994
references.citation: J Gen Virol. 2001 Nov;82(Pt 11):2791-2797
references.pmid: 11602791
references.citation: Cancer Res. 2001 Mar 1;61(5):1768-75
references.pmid: 11280720
references.citation: Cell. 1991 Feb 8;64(3):615-23
references.pmid: 1991323
references.citation: Protein Sci. 1996 May;5(5):895-903
references.pmid: 8732761
references.citation: Proc Natl Acad Sci U S A. 2004 May 18;101(20):7600-5
references.pmid: 15133147
references.citation: Nat Biotechnol. 2003 Aug;21(8):885-90
references.pmid: 12833071
references.citation: Nature. 1994 Aug 4;370(6488):389-91
references.pmid: 8047147
references.citation: Nat Med. 1999 Jan;5(1):29-33
references.pmid: 9883836
references.citation: Biotechnol Bioeng. 2008 Feb 1;99(2):475-84
references.pmid: 17680664
references.citation: Curr Opin Cell Biol. 1995 Jun;7(3):310-8
references.pmid: 7662359
references.citation: PLoS One. 2008 Jun 25;3(6):e2547
references.pmid: 18575629
references.citation: Curr Opin Biotechnol. 2004 Aug;15(4):291-7
references.pmid: 15296927
references.citation: Nature. 1984 Jan 12-18;307(5947):178-80
references.pmid: 6318124
references.citation: Bioconjug Chem. 2000 Nov-Dec;11(6):901-9
references.pmid: 11087340
references.citation: Biochem Biophys Res Commun. 2010 Jan 29;392(1):9-15
references.pmid: 20043881
references.citation: Pharm Res. 2001 Jan;18(1):67-74
references.pmid: 11336355
references.citation: Methods Mol Biol. 2010;673:223-30
references.pmid: 20835802
references.citation: Eur J Pharm Sci. 2003 Sep;20(1):1-16
references.pmid: 13678788
references.citation: J Bacteriol. 1994 Sep;176(17):5474-82
references.pmid: 8071226
references.citation: J Virol. 2005 Oct;79(20):12999-3006
references.pmid: 16189002
references.citation: Neurobiol Dis. 2010 Jan;37(1):48-57
references.pmid: 19664710
references.citation: Cell Mol Life Sci. 1999 Nov 15;56(7-8):580-603
references.pmid: 11212308
references.citation: Vaccine. 2007 Nov 19;25(47):8036-48
references.pmid: 17936444
references.citation: J Nanobiotechnology. 2006 Feb 13;4:2
references.pmid: 16476163
references.citation: Science. 1999 Sep 3;285(5433):1569-72
references.pmid: 10477521
references.citation: Science. 1998 Jan 16;279(5349):377-80
references.pmid: 9430587
references.citation: Expert Opin Drug Discov. 2010 Feb;5(2):123-34
references.pmid: 22822912
references.citation: Nucleic Acids Res. 2004 Feb 27;32(4):1448-59
references.pmid: 14990750
references.citation: Somat Cell Mol Genet. 2002 Nov;27(1-6):27-47
references.pmid: 12774939
references.citation: Adv Drug Deliv Rev. 2008 Mar 1;60(4-5):517-29
references.pmid: 18037527
references.citation: Glycobiology. 2003 Mar;13(3):179-89
references.pmid: 12626416
references.citation: Mol Ther. 2002 Feb;5(2):104-14
references.pmid: 11829517
references.citation: J Control Release. 2008 Nov 24;132(1):21-5
references.pmid: 18727945
references.citation: Nat Biotechnol. 2003 Oct;21(10):1171-8
references.pmid: 14520402
references.citation: J Biol Chem. 1997 Jun 20;272(25):16010-7
references.pmid: 9188504
references.citation: Gene Ther. 2010 Aug;17(8):1033-41
references.pmid: 20410928
references.citation: J Biomed Sci. 2010 Jun 24;17:51
references.pmid: 20573280
references.citation: Hum Gene Ther. 1999 Jan 20;10(2):319-32
references.pmid: 10022556
references.citation: Expert Opin Biol Ther. 2007 Jul;7(7):997-1007
references.pmid: 17665989
references.citation: Methods. 2006 Sep;40(1):60-5
references.pmid: 16997714
references.citation: FEBS Lett. 2005 Apr 11;579(10):2143-8
references.pmid: 15811332
references.citation: J Clin Invest. 2001 Aug;108(3):415-23
references.pmid: 11489935
references.citation: Trends Biochem Sci. 2004 Nov;29(11):618-25
references.pmid: 15501681
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7509-14
references.pmid: 20308536
references.citation: Science. 2008 Feb 22;319(5866):1083-6
references.pmid: 18292340
references.citation: Proc Natl Acad Sci U S A. 1970 Sep;67(1):37-40
references.pmid: 4318785
references.citation: Virology. 1987 Apr;157(2):440-8
references.pmid: 3029982
references.citation: J Biotechnol. 2010 Oct 15;150(2):224-31
references.pmid: 20797415
references.citation: Chem Biol Drug Des. 2008 Aug;72(2):97-110
references.pmid: 18624812
references.citation: In Vivo. 2006 May-Jun;20(3):319-24
references.pmid: 16724664
references.citation: J Biol Chem. 1998 Apr 10;273(15):8835-41
references.pmid: 9535863
references.citation: Angew Chem Int Ed Engl. 2007;46(18):3184-93
references.pmid: 17348058
references.citation: Nat Struct Mol Biol. 2008 Sep;15(9):939-47
references.pmid: 19172747
references.citation: Curr Opin Biotechnol. 1999 Oct;10(5):411-21
references.pmid: 10508629
references.citation: Biochemistry. 1990 Sep 18;29(37):8720-8
references.pmid: 2271552
references.citation: Adv Drug Deliv Rev. 2006 Dec 1;58(14):1505-22
references.pmid: 17118484
references.citation: Arch Microbiol. 2003 Nov;180(5):353-61
references.pmid: 14504694
references.citation: Cancer Biol Ther. 2011 Jan 15;11(2):263-76
references.pmid: 21088503
references.citation: J Control Release. 2007 Sep 11;122(1):1-9
references.pmid: 17651855
references.citation: Antivir Ther. 2004 Aug;9(4):479-89
references.pmid: 15456078
references.citation: J Invest Dermatol. 2007 Nov;127(11):2497-8
references.pmid: 17934501
references.citation: J Control Release. 2007 Apr 2;118(2):216-24
references.pmid: 17239466
references.citation: Appl Microbiol Biotechnol. 2004 Sep;65(4):363-72
references.pmid: 15480623
references.citation: J Bacteriol. 2002 Mar;184(5):1253-61
references.pmid: 11844753
references.citation: Immunotherapy. 2009 Mar;1(2):303-12
references.pmid: 20635947
references.citation: Mol Ther. 2009 May;17(5):767-77
references.pmid: 19277019
references.citation: Trends Biotechnol. 2004 Jul;22(7):371-7
references.pmid: 15245910
references.citation: J Immunol. 2001 May 1;166(9):5346-55
references.pmid: 11313370
references.citation: Science. 2005 Aug 5;309(5736):936-8
references.pmid: 16081736
references.citation: Mol Cell. 2010 Apr 23;38(2):305-15
references.pmid: 20417607
references.citation: Mol Ther. 2006 Sep;14(3):343-50
references.pmid: 16765648
references.citation: Clin Immunol. 2006 Nov;121(2):159-76
references.pmid: 16920030
references.citation: Biochem Biophys Res Commun. 2005 Mar 25;328(4):1182-7
references.pmid: 15708001
references.citation: Cancer Res. 2002 Apr 1;62(7):2013-8
references.pmid: 11929818
references.citation: Cell. 2006 Feb 24;124(4):729-40
references.pmid: 16497584
references.citation: J Biol Chem. 2008 Jan 4;283(1):529-540
references.pmid: 17974567
references.citation: J Am Chem Soc. 2006 Apr 12;128(14):4516-7
references.pmid: 16594656
references.citation: Trends Microbiol. 2003 Sep;11(9):438-44
references.pmid: 13678860
references.citation: Cell Mol Life Sci. 2010 Mar;67(5):715-26
references.pmid: 19898741
references.citation: Nanomedicine (Lond). 2010 Feb;5(2):259-68
references.pmid: 20148637
references.citation: Science. 1973 Nov 9;182(4112):584-6
references.pmid: 4355679
references.citation: Expert Rev Vaccines. 2010 Oct;9(10):1149-76
references.pmid: 20923267
references.citation: Cell. 2008 Aug 22;134(4):577-86
references.pmid: 18691745
references.citation: J Med Virol. 2002 Dec;68(4):537-43
references.pmid: 12376962
references.citation: Methods Mol Biol. 2010;614:111-24
references.pmid: 20225039
references.citation: Biol Chem. 2003 Feb;384(2):247-55
references.pmid: 12675518
references.citation: J Control Release. 2010 May 21;144(1):39-45
references.pmid: 20117154
references.citation: J Bacteriol. 1995 Dec;177(24):7119-24
references.pmid: 8522518
references.citation: J Infect Dis. 2006 Sep 15;194(6):790-4
references.pmid: 16941345
references.citation: Curr Opin Mol Ther. 2003 Oct;5(5):524-8
references.pmid: 14601522
references.citation: Biomaterials. 2010 Aug;31(22):5805-12
references.pmid: 20452667
references.citation: J Mol Med (Berl). 2007 Feb;85(2):191-201
references.pmid: 17111132
references.citation: Blood. 2001 Apr 15;97(8):2221-9
references.pmid: 11290582
references.citation: Virus Genes. 2007 Jun;34(3):303-14
references.pmid: 16927120
references.citation: Nanomedicine. 2006 Sep;2(3):137-49
references.pmid: 17292136
references.citation: Arch Virol. 2008;153(3):585-9
references.pmid: 18175039
references.citation: Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4086-90
references.pmid: 8171039
references.citation: Circ Res. 2007 Jun 8;100(11):1626-33
references.pmid: 17495229
references.citation: J Biol Chem. 2004 Feb 13;279(7):5555-64
references.pmid: 14607832
references.citation: J Biol Chem. 2003 Jan 3;278(1):585-90
references.pmid: 12411431
references.citation: J Bacteriol. 1999 Sep;181(17):5317-29
references.pmid: 10464203
references.citation: Nanotechnology. 2011 Mar 11;22(10):105708
references.pmid: 21289408
references.citation: Hepatology. 2005 Dec;42(6):1300-9
references.pmid: 16317706
references.citation: Biophys Chem. 2003 Oct 1;106(1):23-9
references.pmid: 14516909
references.citation: Nat Biotechnol. 2010 Jan;28(1):79-82
references.pmid: 20037580
references.citation: BioDrugs. 2008;22(5):315-29
references.pmid: 18778113
references.citation: Methods. 2006 Sep;40(1):98-117
references.pmid: 16997718
references.citation: J Control Release. 2002 Sep 18;83(1):169-82
references.pmid: 12220848
references.citation: FASEB J. 2009 Sep;23(9):2996-3006
references.pmid: 19395475
references.citation: J Biol Chem. 2001 Dec 14;276(50):47550-5
references.pmid: 11600500
references.citation: Biomaterials. 2010 Sep;31(27):7106-14
references.pmid: 20619792
references.citation: J Immunol. 2002 Mar 1;168(5):2393-401
references.pmid: 11859130
references.citation: Biochem Biophys Res Commun. 2000 Nov 19;278(2):455-61
references.pmid: 11097858
references.citation: Curr Opin Mol Ther. 1999 Apr;1(2):197-203
references.pmid: 11715943
references.citation: EMBO J. 1988 Jun;7(6):1605-14
references.pmid: 3168999
references.citation: Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):664-8
references.pmid: 8290579
references.citation: Science. 2010 Jan 1;327(5961):81-4
references.pmid: 20044574
references.citation: EMBO J. 1992 Sep;11(9):3263-9
references.pmid: 1505517
references.citation: PLoS Biol. 2004 Feb;2(2):E36
references.pmid: 14966535
references.citation: EMBO J. 1997 Aug 15;16(16):5139-48
references.pmid: 9305654
references.citation: Int J Cancer. 2009 Jan 1;124(1):150-6
references.pmid: 18839427
references.citation: J Control Release. 2005 Oct 20;107(3):562-70
references.pmid: 16139384
references.citation: J Gene Med. 2005 Dec;7(12):1545-54
references.pmid: 16037993
references.citation: J Biol Chem. 2007 Oct 5;282(40):29323-35
references.pmid: 17675289
references.citation: Vaccine. 2008 Oct 29;26(46):5814-21
references.pmid: 18786589
references.citation: J Biol Chem. 1994 Apr 29;269(17):12918-24
references.pmid: 8175709
references.citation: Proc Natl Acad Sci U S A. 1999 May 11;96(10):5486-91
references.pmid: 10318910
references.citation: Biochemistry. 1987 Jun 2;26(11):2964-72
references.pmid: 2886149
references.citation: Gene Ther. 2004 Apr;11(7):628-35
references.pmid: 14973544
references.citation: Curr Pharm Des. 2009;15(8):893-916
references.pmid: 19275653
references.citation: Methods. 2006 Sep;40(1):66-76
references.pmid: 16997715
references.citation: Plant Physiol. 1992 Oct;100(2):784-93
references.pmid: 16653059
references.citation: Bioconjug Chem. 2005 Nov-Dec;16(6):1572-9
references.pmid: 16287257
references.citation: Cancer Res. 2003 Aug 15;63(16):5046-53
references.pmid: 12941833
references.citation: J Gen Virol. 1999 Jan;80 ( Pt 1):39-46
references.pmid: 9934681
references.citation: Nucleic Acids Res. 2007;35(7):e49
references.pmid: 17341467
references.citation: J Drug Target. 2010 Apr;18(3):157-67
references.pmid: 20064077
references.citation: Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13003-8
references.pmid: 11087855
references.citation: Ultrason Sonochem. 2007 Jul;14(5):661-666
references.pmid: 17208504
references.citation: Adv Drug Deliv Rev. 2001 Dec 3;53(1):75-94
references.pmid: 11733118
references.citation: J Cell Sci. 1999 Jun;112 ( Pt 12):2033-41
references.pmid: 10341220
references.citation: Nat Biotechnol. 2005 Jun;23(6):709-17
references.pmid: 15908939
references.citation: FASEB J. 2001 Jun;15(8):1451-3
references.pmid: 11387254
references.citation: Intervirology. 2002;45(1):24-32
references.pmid: 11937768
references.citation: Nucleic Acids Res. 2009 Aug;37(14):4559-69
references.pmid: 19483097
references.citation: Methods Mol Biol. 2007;399:181-98
references.pmid: 18309933
references.citation: J Biol Chem. 1996 May 3;271(18):10560-8
references.pmid: 8631856
references.citation: Curr Opin Struct Biol. 2000 Apr;10(2):229-35
references.pmid: 10753814
references.citation: J Biotechnol. 2005 Jan 26;115(2):113-28
references.pmid: 15607230
references.citation: J Biol Chem. 1994 Apr 8;269(14):10444-50
references.pmid: 8144628
references.citation: Cancer Res. 2005 Dec 15;65(24):11631-8
references.pmid: 16357174
references.citation: Hypertension. 2001 Feb;37(2 Pt 2):449-55
references.pmid: 11230317
references.citation: Curr Med Chem. 2004 Jun;11(11):1375-89
references.pmid: 15180572
references.citation: J Bacteriol. 2006 Apr;188(8):2865-74
references.pmid: 16585748
references.citation: Brain Res Mol Brain Res. 1999 Jun 8;69(2):249-62
references.pmid: 10366746
references.citation: Adv Drug Deliv Rev. 2004 Sep 22;56(11):1537-63
references.pmid: 15350288
references.citation: J Neurosci. 2004 Aug 4;24(31):6880-8
references.pmid: 15295022
references.citation: Science. 1983 May 13;220(4598):725-7
references.pmid: 6301016
references.citation: Gene Ther. 2001 Jan;8(2):166-71
references.pmid: 11313786
references.citation: Gene Ther. 2006 Sep;13(18):1313-9
references.pmid: 16953249
references.citation: Hum Vaccin. 2008 Jan-Feb;4(1):5-12
references.pmid: 18438104
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12128-33
references.pmid: 18713866
references.citation: J Biol Chem. 2003 Oct 24;278(43):42625-36
references.pmid: 12882958
references.citation: Curr Top Microbiol Immunol. 2009;327:123-41
references.pmid: 19198573
references.citation: J Bacteriol. 2008 Apr;190(8):2966-71
references.pmid: 18296526
references.citation: J Virol Methods. 2002 Aug;105(1):147-57
references.pmid: 12176152
references.citation: Plant Physiol. 1992 Oct;100(2):794-800
references.pmid: 16653060
references.citation: Biotechnol Bioeng. 2007 Aug 15;97(6):1376-86
references.pmid: 17286273
references.citation: Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15889-94
references.pmid: 14673108
references.citation: Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17867-72
references.pmid: 15601762
references.citation: Eur J Nucl Med Mol Imaging. 2007 Feb;34(2):267-73
references.pmid: 16909226
references.citation: Int J Pharm. 1999 Aug 20;185(2):129-88
references.pmid: 10460913
references.citation: Biochem Biophys Res Commun. 2001 Jul 13;285(2):201-6
references.pmid: 11444826
references.citation: Biotechnol Bioeng. 2008 Nov 1;101(4):654-64
references.pmid: 18814295
references.citation: Int J Cancer. 2001 Apr 15;92(2):285-93
references.pmid: 11291058
references.citation: Nature. 2007 Jul 5;448(7149):39-43
references.pmid: 17572664
references.citation: Curr Gene Ther. 2006 Feb;6(1):59-71
references.pmid: 16475946
references.citation: Anticancer Drugs. 2008 Oct;19(9):899-909
references.pmid: 18766004
references.citation: Nat Med. 2010 Mar;16(3):334-8
references.pmid: 20111039
references.citation: Expert Rev Vaccines. 2009 Oct;8(10):1379-98
references.pmid: 19803760
references.citation: Adv Drug Deliv Rev. 2005 Feb 28;57(4):559-77
references.pmid: 15722164
references.citation: Blood. 2005 Feb 15;105(4):1424-30
references.pmid: 15507527
references.citation: Adv Drug Deliv Rev. 2008 May 22;60(8):915-28
references.pmid: 18325628
references.citation: Nat Med. 2002 Jul;8(7):751-5
references.pmid: 12053175
references.citation: Mol Ther. 2007 Sep;15(9):1587-92
references.pmid: 17579573
references.citation: Nat Rev Drug Discov. 2006 Feb;5(2):115-21
references.pmid: 16521330
references.citation: Annu Rev Biophys. 2010;39:185-205
references.pmid: 20192762
references.citation: Science. 1997 Oct 3;278(5335):82-7
references.pmid: 9311930
references.citation: Antiviral Res. 2009 Feb;81(2):113-22
references.pmid: 19007818
references.citation: Curr Pharm Biotechnol. 2005 Feb;6(1):49-55
references.pmid: 15727555
references.citation: FASEB J. 1998 Jan;12(1):67-77
references.pmid: 9438412
references.citation: J Bacteriol. 2008 Feb;190(3):936-45
references.pmid: 17993516
references.citation: Virology. 2005 Mar 30;334(1):17-27
references.pmid: 15749119
references.citation: J Exp Med. 1997 Jul 7;186(1):139-46
references.pmid: 9207008
references.citation: Transl Oncol. 2009 May;2(2):59-64
references.pmid: 19412420
references.citation: Vaccine. 2010 Jul 5;28(30):4771-6
references.pmid: 20470801
references.citation: J Natl Cancer Inst. 2001 Jul 18;93(14):1062-74
references.pmid: 11459867
references.citation: Mol Pharm. 2007 Sep-Oct;4(5):631-51
references.pmid: 17880166
references.citation: Annu Rev Biophys Biomol Struct. 2000;29:1-26
references.pmid: 10940241
references.citation: FEBS Lett. 2001 Jun 15;499(1-2):137-42
references.pmid: 11418128
references.citation: Intervirology. 2002;45(4-6):308-17
references.pmid: 12602348
references.citation: J Bacteriol. 1995 Oct;177(19):5434-9
references.pmid: 7559326
references.citation: Angew Chem Int Ed Engl. 2009;48(22):3924-77
references.pmid: 19415701
references.citation: Genome Biol. 2007;8(9):227
references.pmid: 17903313
references.citation: Nat Med. 1998 Aug;4(8):939-44
references.pmid: 9701247
references.citation: Expert Opin Drug Metab Toxicol. 2011 Feb;7(2):201-11
references.pmid: 21241201
references.citation: J Biol Chem. 1997 Feb 14;272(7):3999-4005
references.pmid: 9020106
references.citation: Virology. 2009 Jan 5;383(1):12-21
references.pmid: 18986663
references.citation: J Biomol NMR. 1997 Dec;10(4):397-401
references.pmid: 9460244
references.citation: Biochem Biophys Res Commun. 2003 May 16;304(4):625-31
references.pmid: 12727199
references.citation: J Biol Chem. 2004 Jun 25;279(26):27735-42
references.pmid: 15069063
references.citation: Protein Sci. 2001 Feb;10(2):434-44
references.pmid: 11266629
references.citation: Curr Opin Biotechnol. 2004 Dec;15(6):513-7
references.pmid: 15560977
references.citation: Bioconjug Chem. 2008 Jan;19(1):70-5
references.pmid: 18001077
references.citation: J Pept Res. 1999 Oct;54(4):311-8
references.pmid: 10532236
references.citation: FEBS Lett. 2003 Feb 27;537(1-3):215-21
references.pmid: 12606060
references.citation: Nat Biotechnol. 1999 Sep;17(9):873-7
references.pmid: 10471928
references.citation: Biomol Eng. 2005 Jun;22(1-3):1-9
references.pmid: 15857778
references.citation: Biophys J. 2005 Jan;88(1):25-36
references.pmid: 15475582
references.citation: Nanomedicine (Lond). 2011 Aug;6(6):1047-61
references.pmid: 21651444
references.citation: J Gene Med. 2004 Feb;6(2):185-94
references.pmid: 14978772
references.citation: Biotechnol Bioeng. 2007 Feb 1;96(2):381-91
references.pmid: 16937408
references.citation: Mol Cell Biol. 1987 Jul;7(7):2451-6
references.pmid: 3614197
references.citation: Drug Discov Today. 2005 Aug 1;10(15):1031-9
references.pmid: 16055019
references.citation: Trends Cell Biol. 1998 Feb;8(2):84-7
references.pmid: 9695814
references.citation: J Neurosci Res. 2007 Dec;85(16):3567-73
references.pmid: 17671991
references.citation: Trends Biotechnol. 2005 Oct;23(10):523-9
references.pmid: 16084615
references.citation: Science. 1999 Oct 1;286(5437):120-3
references.pmid: 10506559
references.citation: Gene Ther. 1996 May;3(5):448-57
references.pmid: 9156807
references.citation: Expert Rev Vaccines. 2010 Mar;9(3):299-307
references.pmid: 20218858
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):91-6
references.pmid: 9874777
references.citation: Biochem Biophys Res Commun. 1993 Jul 30;194(2):876-84
references.pmid: 8343170
references.citation: Gene Ther. 2005 Nov;12(21):1581-90
references.pmid: 16034460
references.citation: Gene Ther. 1996 Dec;3(12):1133-42
references.pmid: 8986440
references.citation: BMC Neurosci. 2006 Apr 25;7:35
references.pmid: 16638118
references.citation: Biomacromolecules. 2003 May-Jun;4(3):472-6
references.pmid: 12741758
references.citation: Biotechnol Bioeng. 2008 Jun 1;100(2):387-96
references.pmid: 18080333
references.citation: J Bacteriol. 1999 Oct;181(19):5967-75
references.pmid: 10498708
references.citation: Bioconjug Chem. 1998 Mar-Apr;9(2):260-7
references.pmid: 9548543