title: Elevated micronuclei frequency in type 2 diabetes with high glycosylated hemoglobin.
abstract: OBJECTIVE
  The role of oxidative damage to DNA due to hyperglycemia is well known. In the current study we have evaluated the induction of micronuclei due to increased glycosylation in type 2 diabetes.
  
  
  METHODS
  Forty-nine subjects divided into two groups of normoglycemic controls and type 2 diabetic cases were recruited in the study. Whole blood was cultured and micronuclei were scored in all the cases. This was correlated with age, sex, blood glucose levels and glycosylated hemoglobin.
  
  
  RESULTS
  Age and sex matched diabetic patients had an increased micronuclei frequency in response to elevated glycosylation of hemoglobin (R(2)=0.229, p=0.037) compared to normoglycemic subjects.
  
  
  CONCLUSIONS
  The increased glycosylation seems to induce oxidative damage in the DNA of the diabetic patients, which manifests as an increased micronuclei frequency. This has a potential to be used as a biomarker for subsequent diabetic complications.
authors.affiliation: Cytogenetics and Molecular Genetics Section, Pathology Unit, Medical Division, BARC Hospital, Anushaktinagar, Mumbai 400094, India. sureshs@barc.gov.in
authors.forename: Suresh K G
authors.initials: SK
authors.lastname: Shettigar
authors.forename: C
authors.initials: C
authors.lastname: Shailaja
authors.forename: Ratnakar K
authors.initials: RK
authors.lastname: Kulkarni
mesh_terms: D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D006943:Hyperglycemia / Q000235:genetics* / Q000378:metabolism; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin