title: Levels of some antioxidant molecules and lipid peroxidation during in vivo transformation of the progenetic metacercaria of Clinostomum complanatum to ovigerous adult worms.
abstract: The levels of oxidative stress markers are an important indicator of the physiological state of the parasite and its host. In the present study levels of lipid peroxidation, glutathione S transferase, glutathione, superoxide dismutase and catalase were determined in the Clinostomum complanatum progenetic metacercaria, obtained from the fish peritoneum (a hypoxic habitat). The in vivo transformed ovigerous adult worms were obtained from the aerobic environment of the buccopharyngeal region of experimentally infected chickens. Levels of antioxidant molecules were also determined in the blood of experimentally infected chickens. An increase in the levels of lipid peroxidation, and a significant decrease in the levels of glutathione S transferase, glutathione, superoxide dismutase and catalase was observed in the infected host as compared to the controls. In the ovigerous worms, the levels of lipid peroxidation, glutathione S transferase, glutathione, superoxide dismutase were found to be significantly less than the levels observed in the progenetic metacercaria. Since the establishment of worm in the buccal cavity of the avian host would lead to its exposure to oxygen and the haematophagous nature of the parasite also exposes it to the free radicals in the host blood, the progenetic metacercaria has evolved to produce excess free radical scavenging molecules reserved to combat the oxidative stress encountered within the microhabitat of the definitive host.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors.forename: Asim
authors.initials: A
authors.lastname: Rizvi
authors.forename: Shirin
authors.initials: S
authors.lastname: Hasan
authors.forename: Maroof
authors.initials: M
authors.lastname: Alam
authors.forename: Atif
authors.initials: A
authors.lastname: Zafar
authors.forename: Tasneem
authors.initials: T
authors.lastname: Fatima
authors.forename: P A A
authors.initials: PA
authors.lastname: Shareef
authors.forename: Naheed
authors.initials: N
authors.lastname: Banu
authors.forename: M
authors.initials: M
authors.lastname: Saleemuddin
authors.forename: Mohammad K
authors.initials: MK
authors.lastname: Saifullah
authors.forename: S M A
authors.initials: SM
authors.lastname: Abidi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002645:Chickens; D015227:Lipid Peroxidation / Q000502:physiology*; D058488:Metacercariae / Q000502:physiology*; D018384:Oxidative Stress; D011201:Poultry Diseases / Q000469:parasitology; D014200:Trematoda / Q000502:physiology*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
chemical_list: D000975:Antioxidants