title: Design and construction of synthetic gene networks in mammalian cells.
abstract: Advances in the development of molecular tools for the inducible control of transcription, translation, and protein degradation are the basis for the rapidly emerging design and construction of synthetic gene networks in mammalian cells.In this chapter, we describe such tools and how they can be integrated into a synthetic gene network with desired functionality. The network design and construction process is illustrated in the form of a detailed protocol for the implementation of a logic NOR gate based on an inducible promoter combined with an inducible protein degradation system.
authors.affiliation: Faculty of Biology, BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Karlsson
authors.forename: Wilfried
authors.initials: W
authors.lastname: Weber
authors.forename: Martin
authors.initials: M
authors.lastname: Fussenegger
mesh_terms: D015153:Blotting, Western; D018929:Cell Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D044783:F-Box Proteins / Q000378:metabolism; D053263:Gene Regulatory Networks / Q000187:drug effects / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D007210:Indoleacetic Acids / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D015203:Reproducibility of Results; D058615:Synthetic Biology / Q000379:methods*; D013752:Tetracycline / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection
chemical_list: D044783:F-Box Proteins; D007210:Indoleacetic Acids; D016271:Proto-Oncogene Proteins c-myc; D013752:Tetracycline