title: Maspin increases Ku70 acetylation and Bax-mediated cell death in cancer             cells.
abstract: Ku70, a DNA repair protein, was recently identified as a critical anti-apoptotic protein that inhibits Bax translocation to mitochondria. The dissociation of Bax from Ku70 is essential for the apoptotic activity of Bax. Here, we show that maspin, a tumor suppressor protein frequently lost in cancer, regulates this process. Maspin increased cell death in a Ku70 acetylation-dependent manner. Maspin inhibited histone deacetylase 1 (HDAC1) and thus increased the acetylation of Ku70 and the dissociation of Bax from Ku70, which led to the induction of apoptosis. These results reveal maspin as a Ku70-interacting molecule and provide the basis for a new endogenous acetylation-based control mechanism that reduces Ku70-mediated sequestration of Bax from mitochondria.
authors.affiliation: Cancer Genetics Laboratory, Biomedical Research Institute, Samsung                     Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710,                     Republic of Korea.
authors.forename: Sook-Ja
authors.initials: SJ
authors.lastname: Lee
authors.forename: Haerim
authors.initials: H
authors.lastname: Jang
authors.forename: Chaehwa
authors.initials: C
authors.lastname: Park
mesh_terms: D000107:Acetylation; D034961:Antigens, Nuclear / Q000378:metabolism*; D016923:Cell Death; D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D000072200:Ku Autoantigen; D008239:Lysine / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D015843:Serpins / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; C085344:SERPIN-B5; D015843:Serpins; D051028:bcl-2-Associated X Protein; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen; D008239:Lysine