title: Apoptosis induced by ZnPcH1-based photodynamic therapy in Jurkat cells and HEL cells.
abstract: Photodynamic therapy (PDT) can selectively and effectively kill tumor cells, and photosensitization is the key to these anti-tumor effects. In this study, we investigated the killing mechanisms of the photosensitizer ZnPcH1 (a mono-α-substituted zinc(II) phthalocyanine synthesized in China), in the acute lymphoid leukemia cell line Jurkat and the acute erythroleukemia cell line HEL. Results from acridine orange/ethidium bromide fluorescence staining, DNA gel electrophoresis, and Annexin-V(FITC/PI) double-stained flow cytometry analysis indicated that ZnPcH1-PDT induced apoptosis in Jurkat and HEL cells, with Jurkat cells being more sensitive. Following ZnPcH1-PDT treatment, upregulation of p53 and Bax, downregulation of HSP70, Bcl-2 and Akt, and inhibition of the phosphorylation of Akt and GSK3β were observed. Our results establish a theoretical basis for the application of ZnPcH1-PDT in the treatment of acute leukemia.
authors.affiliation: Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou 350001, People's Republic of China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Huang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D019169:Jurkat Cells; D009942:Organometallic Compounds / Q000494:pharmacology*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013194:Staining and Labeling; D017967:Zinc Compounds
chemical_list: D017304:Annexin A5; D051017:Apoptosis Regulatory Proteins; D018840:HSP70 Heat-Shock Proteins; D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3
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