title: Synthesis and NMR elucidation of novel tetrapeptides.
abstract: The synthesis and NMR elucidation of Ala-Val-Pro-Ile and five novel peptide-based derivatives are reported. These peptides mimic the natural second mitochondria-derived activator of caspase (Smac) protein. Purification was achieved using preparative HPLC and the NMR elucidation of all compounds is reported for the first time. A series of overlapping signals were observed in the 1D NMR spectra thus making assignment a difficult task to undertake. The use of 2D NMR techniques with the inclusion of efficient adiabatic symmetrized ROESY proved to be an effective tool in overcoming these difficulties.
authors.affiliation: School of Pharmacy and Pharmacology, University of KwaZulu-Natal, Durban 4001, South Africa.
authors.forename: Maya
authors.initials: M
authors.lastname: Makatini
authors.forename: Thashini
authors.initials: T
authors.lastname: Chetty
authors.forename: Oluseye K
authors.initials: OK
authors.lastname: Onajole
authors.forename: Thavendran
authors.initials: T
authors.lastname: Govender
authors.forename: Patrick
authors.initials: P
authors.lastname: Govender
authors.forename: Glenn E M
authors.initials: GE
authors.lastname: Maguire
authors.forename: Hendrik G
authors.initials: HG
authors.lastname: Kruger
mesh_terms: D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009842:Oligopeptides