title: Interspecies interaction of signal peptide PapR secreted by Bacillus cereus and its effect on production of antimicrobial peptide.
abstract: This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 μM of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.
authors.affiliation: Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Yeo
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Lee
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Cha
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Hahm
mesh_terms: D000595:Amino Acid Sequence; D000898:Antibiosis; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D003470:Culture Media; D005285:Fermentation; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D009154:Mutation; D013025:Glycine max / Q000378:metabolism; D013045:Species Specificity; D015534:Trans-Activators
chemical_list: D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D003470:Culture Media; C466079:PapR protein, Bacillus cereus; D015534:Trans-Activators