title: BJcuL, a lectin purified from Bothrops jararacussu venom, induces apoptosis in human gastric carcinoma cells accompanied by inhibition of cell adhesion and actin cytoskeleton disassembly.
abstract: We show that BJcuL, a lectin purified from Bothrops jararacussu venom, exerts cytotoxic effects to gastric carcinoma cells MKN45 and AGS. This effect was due to the direct interaction with specific glycans on the cells surface and was observed by cell viability decrease, disorganization of actin filaments and apoptosis. In addition, BJcuL was able to reduce tumor cell adhesion to matrigel, what was inhibited by specific carbohydrate or partially inhibited when cells were pre-incubated with matrigel. Our results suggest that BJcuL was able to promote apoptosis in both tumor cells lines and therefore has a prospect for potential use in cancer therapy.
authors.affiliation: Group for Advanced Molecular Investigation, PPGCS/CCBS, PUCPR, 80215-901 Curitiba, Paraná, Brazil.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Nolte
authors.forename: Danusa
authors.initials: D
authors.lastname: de Castro Damasio
authors.forename: Adriana Cristina
authors.initials: AC
authors.lastname: Baréa
authors.forename: Joana
authors.initials: J
authors.lastname: Gomes
authors.forename: Ana
authors.initials: A
authors.lastname: Magalhães
authors.forename: Luciana F C
authors.initials: LF
authors.lastname: Mello Zischler
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Stuelp-Campelo
authors.forename: Selene L
authors.initials: SL
authors.lastname: Elífio-Esposito
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: Celso A
authors.initials: CA
authors.lastname: Reis
authors.forename: Andréa Novais
authors.initials: AN
authors.lastname: Moreno-Amaral
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D017837:Bothrops; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D003435:Crotalid Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D037181:Lectins, C-Type / Q000302:isolation & purification; D013274:Stomach Neoplasms
chemical_list: D000970:Antineoplastic Agents; C498078:BJcuL lectin, Bothrops jararacussu; D003435:Crotalid Venoms; D037181:Lectins, C-Type