title: Isolation and characterization of a new repetitive DNA family recently amplified in the Mesoamerican gene pool of the common bean (Phaseolus vulgaris L., Fabaceae).
abstract: The common bean (Phaseolus vulgaris) is one of the most important crop plants. About 50% of its genome is composed of repetitive sequences, but only a little fraction was isolated and characterized so far. In this paper, a new repetitive DNA family from the species, named PvMeso, was isolated and characterized in both gene pools of P. vulgaris (Andean and Mesoamerican) and related species. Two fragments, 1.7 and 2.3 kb long, were cloned from BAC 255F18, which has previously shown a repetitive pattern. The subclone PvMeso-31 showed a terminal block in chromosome 7. This subclone contains a 1,705 bp long, AT-rich repeat with small internal repeats and shares a 1.2 kb region with PvMeso-47, derived from the 2.3 kb fragment. The presence of this repetitive block was restricted to Mesoamerican accessions of the common bean. In P. acutifolius, P. leptostachyus and Andean P. vulgaris, only a faint, 2.3 kb fragment was visualized in Southern experiments. Moreover, in Mesoamerican accessions, two other fragments (1.7 kb and 3.4 kb) were strongly labelled as well. Taken together, our results indicate that PvMeso is a recently emerged, repeat family initially duplicated in chromosome 11, on ancestral Mesoamerican accession, and later amplified in chromosome 7, after the split of the two major gene pools of the common bean.
authors.affiliation: Department of Botany, Laboratory of Plant Cytogenetics, Federal University of Pernambuco, Recife, PE 50670-420, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Ribeiro
authors.forename: Karla G B
authors.initials: KG
authors.lastname: dos Santos
authors.forename: Artur
authors.initials: A
authors.lastname: Fonsêca
authors.forename: Andrea
authors.initials: A
authors.lastname: Pedrosa-Harand
mesh_terms: D018744:DNA, Plant / Q000737:chemistry*; D005788:Gene Pool; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D018744:DNA, Plant
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