title: Serum resistance in Haemophilus parasuis SC096 strain requires outer membrane protein P2 expression.
abstract: Haemophilus parasuis outer membrane protein P2 (OmpP2), the most abundant protein in the outer membrane, has been identified as an antigenic protein and a potential virulence factor. To study the precise function of OmpP2, an ompP2-deficient mutant (ΔompP2) of a H. parasuis serovar 4 clinical strain SC096 was constructed by a modified natural transformation system. Compared with the wild-type SC096 strain, the ΔompP2 mutant showed a pronounced growth defect and exhibited significantly greater sensitivity to the bactericidal action of porcine and rabbit sera, whereas the complemented strain could restore the growth and serum resistance phenotypes. The results indicated that H. parasuis OmpP2 from SC096 strain is an important surface protein involved in serum resistance.
authors.affiliation: Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Saixiang
authors.initials: S
authors.lastname: Feng
authors.forename: Chenggang
authors.initials: C
authors.lastname: Xu
authors.forename: Suming
authors.initials: S
authors.lastname: Zhou
authors.forename: Yanbing
authors.initials: Y
authors.lastname: He
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001770:Blood Bactericidal Activity; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D044137:Haemophilus parasuis / Q000276:immunology* / Q000502:physiology*; D008969:Molecular Sequence Data; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013552:Swine; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors