title: Successful preimplantation  genetic aneuploidy screening in Turkish patients.
abstract: Preimplantation  genetic diagnosis is a preventive approach for identifying genetic  abnormalities in early stages of reproduction. We used preimplantation genetic  aneuploidy screening in 230 cycles of patients with indications of advanced  maternal age, recurrent implantation failure, recurrent spontaneous abortions,  or severe male factor. Biopsied blastomeres from embryos with six to eight  blastomeres on day 3 were fixed and fluorescence in situ hybridization  was utilized on chromosomes 13, 16, 18, 21, 22, X, and Y. Among 945  morphologically normal embryos, 314 were diagnosed as chromosomally normal.  Trisomy and monosomy were observed in 36% of the cases (18% each). Embryo  transfer was used in 144 cycles, resulting in 41 pregnancies. Thirty-seven  healthy babies were delivered, with a take-home baby rate of 24.2% and an  implantation rate of 22%. We recommend preimplantation genetic aneuploidy  screening as a valuable technique to select normal chromosome embryos in order  to avoid multiple pregnancies due to the multiple embryo transfers that are  normally necessary to ensure pregnancy in poor prognosis in vitro fertilization  patients.
authors.affiliation: Genetic Disease Diagnostics Center, Istanbul, Turkey. nesrinercelen@gmail.com
authors.forename: N
authors.initials: N
authors.lastname: Ercelen
authors.forename: E
authors.initials: E
authors.lastname: Turtar
authors.forename: M
authors.initials: M
authors.lastname: Gultomruk
authors.forename: H
authors.initials: H
authors.lastname: Comert
authors.forename: H
authors.initials: H
authors.lastname: Coskun
authors.forename: R
authors.initials: R
authors.lastname: Mercan
authors.forename: A
authors.initials: A
authors.lastname: Nuhoglu
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001757:Blastomeres / Q000473:pathology*; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008423:Maternal Age; D008875:Middle Aged; D011247:Pregnancy; D011248:Pregnancy Complications / Q000473:pathology; D019836:Preimplantation Diagnosis / Q000379:methods; D014314:Trisomy; D014421:Turkey