title: Functional characterization of the regulators of calcineurin in Candida glabrata.
abstract: The serine-threonine-specific protein phosphatase calcineurin is a key mediator of various stress responses in fungi. Herein, we characterized functions of the endogenous regulators of calcineurin (RCNs), Rcn1 and Rcn2, in the pathogenic fungus Candida glabrata. Rcn1 exerted both inhibitory and stimulatory effects on calcineurin signaling, but Rcn2 displayed only inhibitory activity. Phenotypic analyses of C. glabrata strains lacking either RCNs, calcineurin, or both revealed that calcineurin requires Rcn1, but not Rcn2, for antifungal tolerance in C. glabrata.
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University School of Medicine, Japan. taiga-m@nagasaki-u.ac.jp
authors.forename: Taiga
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Koichi
authors.initials: K
authors.lastname: Izumikawa
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Nagayoshi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Saijo
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yamauchi
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Morinaga
authors.forename: Masafumi
authors.initials: M
authors.lastname: Seki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kakeya
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D019703:Calcineurin / Q000187:drug effects / Q000378:metabolism*; D065095:Calcineurin Inhibitors; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D025141:Drug Resistance, Fungal / Q000235:genetics; D054714:Echinocandins / Q000494:pharmacology; D015725:Fluconazole / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D055666:Lipopeptides / Q000494:pharmacology; D000077551:Micafungin; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D017384:Sequence Deletion; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013312:Stress, Physiological; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D065095:Calcineurin Inhibitors; D054714:Echinocandins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D055666:Lipopeptides; D012331:RNA, Fungal; D012333:RNA, Messenger; D014415:Tunicamycin; D015725:Fluconazole; D019703:Calcineurin; D000077551:Micafungin