title: The non-toxic and biodegradable adjuvant Montanide ISA 720/CpG can replace Freund's in a cancer vaccine targeting ED-B--a prerequisite for clinical development.
abstract: We have recently shown that immunization against the extra domain-B (ED-B) of fibronectin, using Freund's adjuvant, reduces tumor growth in mice by 70%. In the present study we compare the immune response generated against ED-B using the non-toxic and biodegradable adjuvant Montanide ISA 720/CpG with the response elicited by Freund's adjuvant. Montanide ISA 720/CpG induced anti-ED-B antibodies with higher avidity and less variable levels between individuals than Freund's. Moreover, the duration of the immune response was longer and the generation of anti-ED-B antibodies in naïve mice was faster, when Montanide ISA 720/CpG was used. We conclude that it is possible to replace the mineral oil based adjuvant Freund's with an adjuvant acceptable for human use, which is a prerequisite for transfer of the ED-B vaccine to the clinic.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala Biomedical Center, Husargatan 3, SE-751 23 Uppsala, Sweden.
authors.forename: Elisabeth J M
authors.initials: EJ
authors.lastname: Huijbers
authors.forename: Julia
authors.initials: J
authors.lastname: Femel
authors.forename: Karl
authors.initials: K
authors.lastname: Andersson
authors.forename: Hanna
authors.initials: H
authors.lastname: Björkelund
authors.forename: Lars
authors.initials: L
authors.lastname: Hellman
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Olsson
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000009:adverse effects; D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D005353:Fibronectins / Q000037:antagonists & inhibitors* / Q000276:immunology*; D005620:Freund's Adjuvant / Q000008:administration & dosage* / Q000009:adverse effects; D008353:Mannitol / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D051379:Mice; D008810:Mice, Inbred C57BL; D009829:Oleic Acids / Q000008:administration & dosage* / Q000009:adverse effects; D017434:Protein Structure, Tertiary
chemical_list: D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D019496:Cancer Vaccines; D005353:Fibronectins; D009829:Oleic Acids; C001681:mannide monooleate; D008353:Mannitol; D005620:Freund's Adjuvant