title: MMP(-2) expression in skeletal muscle after strength training.
abstract: The aim of this study was to assess the effects of resistance training on ladders (RTL) on MMP(-2) expression and blood lactate concentration [La-]. 30 male (3 months of age), albino rats were divided into 3 groups: sedentary control (SC, n=10), low resistance exercise training (Low-IntRT, n=10) and high-intensive exercise training (High-IntRT, n=10). Animals of High-IntRT were submitted to a progressively increasing overload in relation to body weight until exhaustion, while the Low-IntRT group performed the same exercise regimen with no external load. The program had a frequency of 3 times per week over 8 weeks. MMP(-2) expression of tibialis anterior muscle and [La-] were measured. While there was a significant increase of MMP(-2) (pro-form) in both groups, only High-IntRT significantly increased MMP(-2) in active-form (p<0.05). Both trained groups exhibited an increase in [La-] when compared to controls, however, the increase in [La-] was significantly higher in the High-IntRT compared to Low-IntRT (p<0.05). Strong correlation was found between MMP(-2) (active form) and [La-] in High-IntRT (r=0.91). RTL in using low and high-intensity exercise can serve as a model to demonstrate different responses of MMP(-2) expression in an animal model. It appears active form expression of MMP(-2) is modulated by exercise intensity.
authors.affiliation: UFSCar, Physiotherapy, Sao Carlos, Brazil.
authors.forename: A P L
authors.initials: AP
authors.lastname: Deus
authors.forename: D
authors.initials: D
authors.lastname: Bassi
authors.forename: R P
authors.initials: RP
authors.lastname: Simões
authors.forename: C R
authors.initials: CR
authors.lastname: Oliveira
authors.forename: V
authors.initials: V
authors.lastname: Baldissera
authors.forename: R de Cássia
authors.initials: Rde C
authors.lastname: Marqueti
authors.forename: H S S
authors.initials: HS
authors.lastname: Araujo
authors.forename: R
authors.initials: R
authors.lastname: Arena
authors.forename: A
authors.initials: A
authors.lastname: Borghi-Silva
mesh_terms: D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D019344:Lactic Acid / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats
chemical_list: D019344:Lactic Acid; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat