title: Characterization of intrinsically disordered prostate associated gene (PAGE5) at single residue resolution by NMR spectroscopy.
abstract: BACKGROUND
  The Cancer-Testis antigens (CTA) are proteins expressed in human germ line and certain cancer cells. CTAs form a large gene family, representing 10% of X-chromosomal genes. They have high potential for cancer-specific immunotherapy. However, their biological functions are currently unknown. Prostate associated genes (PAGE) are characterized as CTAs. PAGE5 is one of six proteins belonging to this protein family, also called CT16.
  
  
  RESULTS
  In this study we show, using bioinformatics, chromatographic and solution state NMR spectroscopic methods, that PAGE5 is an intrinsically disordered protein (IDP).
  
  
  CONCLUSIONS
  The study stands out as the first time structural characterization of the PAGE family protein and introduces how solution state NMR spectroscopy can be effectively utilized for identification of molecular recognition regions (MoRF) in IDPs, known often as transiently populated secondary structures.
authors.affiliation: Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Hellman
authors.forename: Helena
authors.initials: H
authors.lastname: Tossavainen
authors.forename: Pekka
authors.initials: P
authors.lastname: Rappu
authors.forename: Jyrki
authors.initials: J
authors.lastname: Heino
authors.forename: Perttu
authors.initials: P
authors.lastname: Permi
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D006801:Humans; D058950:Melanoma-Specific Antigens / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid
chemical_list: D058950:Melanoma-Specific Antigens; C558361:melanoma-derived CT16 cancer testis antigen, human
references.citation: Vaccine. 2007 Sep 27;25 Suppl 2:B61-71
references.pmid: 17916463
references.citation: FEBS Lett. 1997 Dec 15;419(2-3):285-9
references.pmid: 9428652
references.citation: Chemphyschem. 2009 Jul 13;10(9-10):1415-9
references.pmid: 19462392
references.citation: Trends Biochem Sci. 2005 Sep;30(9):484-9
references.pmid: 16054818
references.citation: Biochemistry. 1994 May 17;33(19):5984-6003
references.pmid: 7514039
references.citation: J Biomol NMR. 1995 Jun;5(4):376-82
references.pmid: 7647557
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D816-9
references.pmid: 18838390
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20422-7
references.pmid: 19088187
references.citation: Bioinformatics. 2009 Oct 15;25(20):2745-6
references.pmid: 19717576
references.citation: Curr Opin Chem Biol. 2010 Oct;14(5):652-9
references.pmid: 20822947
references.citation: Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10697-702
references.pmid: 15252201
references.citation: Bioinformatics. 2004 Mar 1;20(4):477-86
references.pmid: 14990443
references.citation: Nat Rev Cancer. 2005 Aug;5(8):615-25
references.pmid: 16034368
references.citation: J Mol Biol. 2004 May 14;338(5):1015-26
references.pmid: 15111064
references.citation: Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10757-62
references.pmid: 9724777
references.citation: J Biomol NMR. 2009 Nov;45(3):301-10
references.pmid: 19768387
references.citation: J Magn Reson. 2004 Oct;170(2):244-51
references.pmid: 15388087
references.citation: J Biomol NMR. 1999 Jan;13(1):83-8
references.pmid: 21080267
references.citation: J Biomol NMR. 1995 Sep;6(2):153-62
references.pmid: 8589604
references.citation: Int J Cancer. 2011 May 15;128(10):2382-92
references.pmid: 20658534
references.citation: J Proteome Res. 2004 Nov-Dec;3(6):1243-53
references.pmid: 15595734
references.citation: Clin Cancer Res. 2007 Nov 1;13(21):6386-95
references.pmid: 17975151
references.citation: Biochemistry. 1996 Feb 27;35(8):2674-86
references.pmid: 8611573
references.citation: Mol Oncol. 2011 Apr;5(2):164-82
references.pmid: 21376678
references.citation: Biophys J. 2010 May 19;98(10):2383-90
references.pmid: 20483348
references.citation: Cancer Biol Ther. 2002 Jul-Aug;1(4):380-7
references.pmid: 12432251
references.citation: Protein Sci. 2006 Dec;15(12):2795-804
references.pmid: 17088319
references.citation: Int J Cancer. 2002 May 20;99(3):361-9
references.pmid: 11992404
references.citation: J Biol Chem. 2011 Apr 22;286(16):13985-94
references.pmid: 21357425
references.citation: J Biomol NMR. 2011 Jun;50(2):157-65
references.pmid: 21604143
references.citation: Bioinformatics. 2005 Aug 15;21(16):3433-4
references.pmid: 15955779
references.citation: Nature. 2005 Mar 17;434(7031):325-37
references.pmid: 15772651
references.citation: J Biomed Biotechnol. 2010;2010:568068
references.pmid: 20011072
references.citation: Int J Cancer. 2002 Apr 1;98(4):485-92
references.pmid: 11920606
references.citation: J Biomol NMR. 2002 May;23(1):47-55
references.pmid: 12061717
references.citation: Magn Reson Chem. 2006 Jul;44 Spec No:S168-76
references.pmid: 16823899
references.citation: J Biomol NMR. 1995 Sep;6(2):135-40
references.pmid: 8589602
references.citation: FEBS Lett. 2005 Jun 13;579(15):3342-5
references.pmid: 15943979
references.citation: Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12837-42
references.pmid: 17652518
references.citation: Trends Biochem Sci. 2008 Jan;33(1):2-8
references.pmid: 18054235
references.citation: J Biol Chem. 1998 Jul 10;273(28):17618-25
references.pmid: 9651357
references.citation: J Biomol NMR. 1998 Feb;11(2):221-6
references.pmid: 9679296
references.citation: J Proteome Res. 2007 Jun;6(6):2351-66
references.pmid: 17488107
references.citation: J Biomol NMR. 2011 Feb;49(2):99-109
references.pmid: 21259120
references.citation: J Biomol NMR. 2010 Jul;47(3):171-81
references.pmid: 20437194