title: Expansins are involved in cell growth mediated by abscisic acid and indole-3-acetic acid under drought stress in wheat.
abstract: Expansin protein is a component of the cell wall generally accepted to be the key regulator of cell wall extension during plant growth. Plant hormones regulate expansin gene expression as well as plant growth during drought stress. However, the relationship between expansin and plant hormone is far from clear. Here, we studied the involvement of expansin in plant cell growth mediated by the hormones indole-3-acetic acid (IAA) and abscisic acid (ABA) under osmotic stress which was induced by polyethylene glycol (PEG)-6000. Wheat coleoptiles from a drought-resistant cultivar HF9703 and a drought-sensitive cultivar 921842 were used to evaluate cell growth and expansin activity. Osmotic stress induced the accumulation of ABA. ABA induced expansin activity mainly by enhancing expansin expression, since ABA induced cell wall basification via decreasing plasma membrane H(+)-ATPase activity, which was unfavorable for expansin activity. Although ABA induced expansin activity and cell wall extension, treatment with exogenous ABA and/or fluridone (FLU, an ABA inhibitor) suggested that ABA was involved in the coleoptile growth inhibition during osmotic stress. IAA application to detached coleoptiles also enhanced coleoptile growth and increased expansin activity, but unlike ABA, IAA-induced expansin activity was mainly due to the decrease of cell wall pH by increasing plasma membrane H(+)-ATPase activity. Compared with drought-sensitive cultivar, the drought-resistant cultivar could maintain greater expansin activity and cell wall extension, which was contributive to its resultant faster growth under water stress.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China.
authors.forename: Mei-rong
authors.initials: MR
authors.lastname: Zhao
authors.forename: Yang-yang
authors.initials: YY
authors.lastname: Han
authors.forename: Ya-nan
authors.initials: YN
authors.lastname: Feng
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D002473:Cell Wall / Q000378:metabolism; D018548:Cotyledon / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D003681:Dehydration; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology; D006540:Herbicides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology*; D009997:Osmotic Pressure / Q000187:drug effects; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011728:Pyridones / Q000494:pharmacology; D018749:RNA, Plant / Q000235:genetics; D013997:Time Factors; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D014867:Water / Q000378:metabolism
chemical_list: D006540:Herbicides; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011728:Pyridones; D018749:RNA, Plant; C095825:expansin protein, plant; D014867:Water; C013351:fluridone; D011092:Polyethylene Glycols; C030737:indoleacetic acid; D000040:Abscisic Acid; D006180:Proton-Translocating ATPases
references.citation: J Integr Plant Biol. 2008 Apr;50(4):427-34
references.pmid: 18713376
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Mol Genet Genomics. 2004 Nov;272(4):433-42
references.pmid: 15480789
references.citation: Plant Cell. 2002;14 Suppl:S165-83
references.pmid: 12045276
references.citation: Plant Mol Biol. 2001 Jul;46(5):581-9
references.pmid: 11516151
references.citation: Plant Physiol. 1992 Feb;98(2):488-95
references.pmid: 16668666
references.citation: Plant Physiol. 2000 Aug;123(4):1399-414
references.pmid: 10938357
references.citation: FEBS Lett. 2004 Feb 13;559(1-3):61-5
references.pmid: 14960308
references.citation: Plant Physiol. 2001 Sep;127(1):315-23
references.pmid: 11553759
references.citation: Planta. 1989 Jan;177(1):121-30
references.pmid: 24212280
references.citation: Nature. 2000 Sep 21;407(6802):321-6
references.pmid: 11014181
references.citation: Plant Physiol. 2001 Aug;126(4):1471-9
references.pmid: 11500546
references.citation: J Plant Physiol. 2011 Jun 15;168(9):960-6
references.pmid: 21316798
references.citation: Plant Physiol. 2001 May;126(1):244-52
references.pmid: 11351087
references.citation: Plant Physiol. 1988 Jan;86(1):28-31
references.pmid: 16665881
references.citation: Plant Physiol. 1994 Jan;104(1):247-254
references.pmid: 12232076
references.citation: Trends Cell Biol. 2006 Aug;16(8):397-402
references.pmid: 16815705
references.citation: Se Pu. 2002 Mar;20(2):148-50
references.pmid: 12541973
references.citation: Mol Genet Genomics. 2005 Dec;274(5):548-56
references.pmid: 16270219
references.citation: J Exp Bot. 2004 Nov;55(407):2343-51
references.pmid: 15448181
references.citation: Plant Physiol. 1995 May;108(1):303-312
references.pmid: 12228475
references.citation: Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9783-8
references.pmid: 10931949
references.citation: Plant Mol Biol. 2002 Jun-Jul;49(3-4):349-56
references.pmid: 12036259
references.citation: Annu Rev Plant Biol. 2002;53:247-73
references.pmid: 12221975
references.citation: Protoplasma. 2011 Apr;248(2):313-23
references.pmid: 20559851
references.citation: Plant Cell. 1992 Nov;4:1425-33
references.pmid: 11538167
references.citation: Plant Physiol. 2003 Dec;133(4):1677-90
references.pmid: 14681531
references.citation: Plant J. 2005 Aug;43(4):597-610
references.pmid: 16098112
references.citation: Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):12186-91
references.pmid: 10518597
references.citation: Plant Cell Rep. 2011 Jan;30(1):27-36
references.pmid: 20976459
references.citation: Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6574-8
references.pmid: 11607483
references.citation: Plant Physiol. 2004 Aug;135(4):2291-300
references.pmid: 15286291
references.citation: Protoplasma. 2008;233(1-2):73-81
references.pmid: 18726548