title: Rapid determination of protein binding constant by a pressure-mediated affinity capillary electrophoresis method.
abstract: A new pressure-mediated affinity capillary electrophoresis method for the rapid and accurate determination of drug-protein binding constants is described. A special combination of pressure and electrophoresis is used to shorten the electrophoresis and the overall analysis time to only a few minutes. At the same time, the suitability of this method is checked against a traditional fluorescence spectroscopy method. The binding constants of bovine serum albumin and a total of eight drugs with different pK(a) have been evaluated and compared with those determined by the fluorescence spectroscopy method and other methods in literature. The results indicate that the P-ACE method is well suited for the determination of binding constants with weak interaction (K(b) <10(5) M(-1)).
authors.affiliation: Tianjin Key Laboratory for Modem Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P R China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: You X
authors.initials: YX
authors.lastname: Li
authors.forename: He N
authors.initials: HN
authors.lastname: Gao
authors.forename: Juan
authors.initials: J
authors.lastname: Bian
authors.forename: James J
authors.initials: JJ
authors.lastname: Bao
mesh_terms: D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism*; D011312:Pressure; D011485:Protein Binding; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine