title: Evaluation of chronic lymphocytic leukemia by oligonucleotide-based microarray analysis uncovers novel aberrations not detected by FISH or cytogenetic analysis.
abstract: BACKGROUND
  Cytogenetic evaluation is a key component of the diagnosis and prognosis of chronic lymphocytic leukemia (CLL). We performed oligonucleotide-based comparative genomic hybridization microarray analysis on 34 samples with CLL and known abnormal karyotypes previously determined by cytogenetics and/or fluorescence in situ hybridization (FISH).
  
  
  RESULTS
  Using a custom designed microarray that targets >1800 genes involved in hematologic disease and other malignancies, we identified additional cryptic aberrations and novel findings in 59% of cases. These included gains and losses of genes associated with cell cycle regulation, apoptosis and susceptibility loci on 3p21.31, 5q35.2q35.3, 10q23.31q23.33, 11q22.3, and 22q11.23.
  
  
  CONCLUSIONS
  Our results show that microarray analysis will detect known aberrations, including microscopic and cryptic alterations. In addition, novel genomic changes will be uncovered that may become important prognostic predictors or treatment targets for CLL in the future.
authors.affiliation: Signature Genomic Laboratories, PerkinElmer Inc,, 2820 North Astor Street, Spokane, WA, 99207, USA. lisa.shaffer@perkinelmer.com.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Kolquist
authors.forename: Roger A
authors.initials: RA
authors.lastname: Schultz
authors.forename: Marilyn L
authors.initials: ML
authors.lastname: Slovak
authors.forename: Lisa D
authors.initials: LD
authors.lastname: McDaniel
authors.forename: Theresa C
authors.initials: TC
authors.lastname: Brown
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Tubbs
authors.forename: James R
authors.initials: JR
authors.lastname: Cook
authors.forename: Karl S
authors.initials: KS
authors.lastname: Theil
authors.forename: Victoria
authors.initials: V
authors.lastname: Cawich
authors.forename: Caitlin
authors.initials: C
authors.lastname: Valentin
authors.forename: Sara
authors.initials: S
authors.lastname: Minier
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Neill
authors.forename: Steve
authors.initials: S
authors.lastname: Byerly
authors.forename: S Annie
authors.initials: SA
authors.lastname: Morton
authors.forename: Trilochan
authors.initials: T
authors.lastname: Sahoo
authors.forename: Blake C
authors.initials: BC
authors.lastname: Ballif
authors.forename: Lisa G
authors.initials: LG
authors.lastname: Shaffer
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2750-5
references.pmid: 17293455
references.citation: J Clin Oncol. 2007 Oct 10;25(29):4648-56
references.pmid: 17925562
references.citation: EMBO J. 1999 Oct 1;18(19):5242-51
references.pmid: 10508158
references.citation: Leukemia. 2009 May;23(5):1011-7
references.pmid: 19158838
references.citation: Leukemia. 2004 Mar;18(3):476-83
references.pmid: 14712287
references.citation: Mol Diagn Ther. 2008;12(5):271-80
references.pmid: 18803425
references.citation: J Mol Diagn. 2009 Jan;11(1):25-34
references.pmid: 19074592
references.citation: J Mol Diagn. 2010 Mar;12(2):184-96
references.pmid: 20075210
references.citation: Cancer Cell. 2010 Jan 19;17(1):3-4
references.pmid: 20129242
references.citation: Blood. 1999 Sep 15;94(6):1840-7
references.pmid: 10477712
references.citation: Int J Cancer. 2010 Apr 1;126(7):1716-23
references.pmid: 19816935
references.citation: Br J Haematol. 2007 Jul;138(2):217-20
references.pmid: 17593029
references.citation: Blood. 2009 Feb 5;113(6):1294-303
references.pmid: 18922857
references.citation: Oncogene. 1999 Oct 14;18(42):5785-94
references.pmid: 10523859
references.citation: Leuk Res. 2009 Sep;33(9):1276-81
references.pmid: 19027161
references.citation: Cell. 1991 Oct 4;67(1):189-96
references.pmid: 1655274
references.citation: Br J Haematol. 2008 Nov;143(4):532-6
references.pmid: 18752589
references.citation: Blood. 2008 Aug 15;112(4):965-74
references.pmid: 18505780
references.citation: Cancer. 1981 Jul 1;48(1):198-206
references.pmid: 7237385
references.citation: Blood. 1999 Jul 15;94(2):448-54
references.pmid: 10397712
references.citation: Br J Haematol. 2010 Feb;148(4):544-50
references.pmid: 19895615
references.citation: Cancer Cell. 2010 Jan 19;17(1):28-40
references.pmid: 20060366
references.citation: Blood Cancer J. 2011 Mar;1(3):e12
references.pmid: 22829125
references.citation: Leukemia. 2011 Mar;25(3):489-97
references.pmid: 21151023
references.citation: Hematology Am Soc Hematol Educ Program. 2004;:163-83
references.pmid: 15561682
references.citation: Carcinogenesis. 2002 Jul;23(7):1139-48
references.pmid: 12117771
references.citation: Hematology Am Soc Hematol Educ Program. 2009;:421-9
references.pmid: 20008228
references.citation: Blood. 2016 Oct 27;128(17):2109
references.pmid: 27789434
references.citation: Blood. 2002 Jun 1;99(11):4216-8
references.pmid: 12010828
references.citation: Am J Hematol. 2008 Jul;83(7):540-6
references.pmid: 18161787
references.citation: Blood. 1999 Sep 15;94(6):1848-54
references.pmid: 10477713
references.citation: N Engl J Med. 2004 Sep 30;351(14):1409-18
references.pmid: 15459302