title: Mussel-inspired anchoring for patterning cells using polydopamine.
abstract: This Article introduces a simple method of cell patterning, inspired by the mussel anchoring protein. Polydopamine (PDA), artificial polymers made from self-polymerization of dopamine (a molecule that resembles mussel-adhesive proteins), has recently been studied for its ability to make modifications on surfaces in aqueous solutions. We explored the interfacial interaction between PDA and poly(ethylene glycol) (PEG) using microcontact printing (μCP). We patterned PDA on several substrates such as glass, polystyrene, and poly(dimethylsiloxane) and realized spatially defined anchoring of mammalian cells as well as bacteria. We applied our system in investigating the relationship between areas of mammalian nuclei and that of the cells. The combination of PDA and PEG enables us to make cell patterns on common laboratorial materials in a mild and convenient fashion.
authors.affiliation: Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing 100080, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Sun
authors.forename: Yunyan
authors.initials: Y
authors.lastname: Xie
authors.forename: Dekai
authors.initials: D
authors.lastname: Ye
authors.forename: Yuyun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Fei
authors.initials: F
authors.lastname: Long
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D032701:Biomimetics / Q000379:methods*; D049872:Bivalvia; D053145:Cell Nucleus Size; D004926:Escherichia coli / Q000166:cytology; D007211:Indoles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011327:Printing / Q000379:methods*; D013212:Staphylococcus epidermidis / Q000166:cytology; D013499:Surface Properties
chemical_list: D007211:Indoles; D008689:Methacrylates; D011108:Polymers; D011137:Polystyrenes; C421283:poly(ethylene glycol)-dimethacrylate; C568283:polydopamine; D011107:Polylysine; D011092:Polyethylene Glycols