title: Interaction between cholesteryl ester transfer protein and hepatic lipase encoding genes and the risk of type 2 diabetes: results from the Telde study.
abstract: OBJECTIVE
  Diabetic dyslipidaemia is common in type 2 diabetes (T2D) and insulin resistance and often precedes the onset of T2D. The Taq1B polymorphism in CETP (B1 and B2 alleles) (rs708272) and the G-250A polymorphism in LIPC (rs2070895) are associated with changes in enzyme activity and lipid concentrations. Our aim was to assess the effects of both polymorphisms on the risk of T2D.
  
  
  RESULTS
  In a case-control study from the population-based Telde cohort, both polymorphisms were analysed by PCR-based methods. Subjects were classified, according to an oral glucose tolerance test, into diabetic (N = 115) and pre-diabetic (N = 116); 226 subjects with normal glucose tolerance, matched for age and gender, were included as controls. Chi-square (comparison between groups) and logistic regression (identification of independent effects) were used for analysis. The B1B1 Taq1B CETP genotype frequency increased with worsening glucose metabolism (42.5%, 46.1% and 54.3% in control, IGR and diabetic group; p = 0.042). This polymorphism was independently associated with an increased risk of diabetes (OR: 1.828; IC 95%: 1.12-2.99; p = 0.016), even after adjusting by confounding variables, whereas the LIPC polymorphism was not. Regarding the interaction between both polymorphisms, in the B1B1 genotype carriers, the absence of the minor (A) allele of the LIPC polymorphism increased the risk of having diabetes.
  
  
  CONCLUSIONS
  The presence of the B1B1 Taq1B CETP genotype contributes to the presence of diabetes, independently of age, sex, BMI and waist. However, among carriers of B1B1, the presence of GG genotype of the -250 LIPC polymorphism increases this risk further.
authors.affiliation: Servicio de Endocrinología, Complejo Hospitalario Universitario Insular Materno-Infantil de Gran Canaria, Las Palmas de Gran Canaria, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: López-Ríos
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Nóvoa
authors.forename: Ricardo
authors.initials: R
authors.lastname: Chirino
authors.forename: Francisco
authors.initials: F
authors.lastname: Varillas
authors.forename: Mauro
authors.initials: M
authors.lastname: Boronat-Cortés
authors.forename: Ana M
authors.initials: AM
authors.lastname: Wägner
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D015331:Cohort Studies; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D017931:DNA Primers; D008049:Lipase
references.citation: Diabet Med. 2006 Feb;23(2):148-55
references.pmid: 16433712
references.citation: Atherosclerosis. 2001 Dec;159(2):451-8
references.pmid: 11730826
references.citation: Eur J Clin Invest. 1999 Feb;29(2):116-28
references.pmid: 10092998
references.citation: Arterioscler Thromb Vasc Biol. 1999 Nov;19(11):2701-7
references.pmid: 10559014
references.citation: Am J Clin Nutr. 2000 Jan;71(1):13-20
references.pmid: 10617941
references.citation: Nutr Metab Cardiovasc Dis. 2008 Feb;18(2):88-95
references.pmid: 17327141
references.citation: Arterioscler Thromb Vasc Biol. 1998 Nov;18(11):1723-9
references.pmid: 9812910
references.citation: Br J Nutr. 2000 Aug;84(2):203-9
references.pmid: 11029971
references.citation: Obesity (Silver Spring). 2008 Apr;16(4):919-22
references.pmid: 18239576
references.citation: J Clin Invest. 1993 Oct;92(4):1650-8
references.pmid: 8408618
references.citation: J Clin Endocrinol Metab. 2004 Aug;89(8):3858-63
references.pmid: 15292318
references.citation: Curr Opin Lipidol. 1999 Aug;10(4):321-7
references.pmid: 10482135
references.citation: Diabet Med. 2007 Sep;24(9):934-45
references.pmid: 17725706
references.citation: J Lipid Res. 2000 Jan;41(1):155-8
references.pmid: 10627514
references.citation: Curr Opin Lipidol. 2004 Aug;15(4):393-8
references.pmid: 15243211
references.citation: Arterioscler Thromb. 1993 Jun;13(6):815-25
references.pmid: 8499401
references.citation: J Clin Invest. 1998 Mar 1;101(5):956-60
references.pmid: 9486964
references.citation: Nutr Metab Cardiovasc Dis. 2011 Jan;21(1):18-24
references.pmid: 19822408
references.citation: J Lipid Res. 1999 Jul;40(7):1294-303
references.pmid: 10393214
references.citation: J Intern Med. 2005 Feb;257(2):185-93
references.pmid: 15656877
references.citation: Clin Genet. 1989 Jan;35(1):49-56
references.pmid: 2564326
references.citation: Arterioscler Thromb. 1993 Aug;13(8):1227-35
references.pmid: 8343498
references.citation: J Am Coll Cardiol. 2003 Jun 4;41(11):1972-82
references.pmid: 12798568
references.citation: Diabetes Care. 2005 Oct;28(10):2388-93
references.pmid: 16186268
references.citation: Atherosclerosis. 2000 Oct;152(2):367-76
references.pmid: 10998464
references.citation: Nutr Metab Cardiovasc Dis. 2006 Jan;16(1):44-53
references.pmid: 16399491
references.citation: Eur J Clin Invest. 1994 Mar;24(3):188-94
references.pmid: 8033953
references.citation: Nucleic Acids Res. 2001 Sep 1;29(17):E88-8
references.pmid: 11522844
references.citation: Curr Opin Lipidol. 2003 Apr;14(2):179-89
references.pmid: 12642787
references.citation: Atherosclerosis. 2011 Jun;216(2):374-80
references.pmid: 21371711
references.citation: Curr Opin Lipidol. 2000 Jun;11(3):277-83
references.pmid: 10882343
references.citation: Arteriosclerosis. 1989 Jul-Aug;9(4):485-92
references.pmid: 2751477
references.citation: J Clin Endocrinol Metab. 2004 May;89(5):2019-23
references.pmid: 15126514
references.citation: J Clin Endocrinol Metab. 2007 Jul;92(7):2680-7
references.pmid: 17440012
references.citation: J Clin Endocrinol Metab. 2008 Jun;93(6):2294-9
references.pmid: 18364377