title: How the concentration of insulin affects the development of preantral follicles in goats.
abstract: This study investigated the effect of adding different insulin concentrations to the culture medium for goat preantral follicle development in vitro. The ovarian fragments were immediately fixed or cultured for 7 days in MEM with insulin (0, 5, 10 ng/ml and 5 or 10 μg/ml). The results showed that, after 7 days of culture, insulin at 10 ng/ml was the best concentration to preserve follicular viability and ultrastructure, resulting in the highest rates of normal follicles. After 7 days, only treatments with 10 ng/ml and 5 μg/ml of insulin increased follicular activation when compared to other concentrations. Regarding follicular and oocyte growth, the presence of 10 ng/ml of insulin promoted a larger diameter than other treatments. In conclusion, this study shows that addition of 10 ng/ml of insulin to the culture medium improved the survival and stimulated growth of goat preantral follicles.
authors.affiliation: Faculty of Veterinary, State University of Ceará, Campus Itaperi, Fortaleza, CE, Brazil. rncvet@gmail.com
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.forename: A M C V
authors.initials: AM
authors.lastname: Alves
authors.forename: L R
authors.initials: LR
authors.lastname: Faustino
authors.forename: K P L
authors.initials: KP
authors.lastname: Oliveira
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D005260:Female; D006041:Goats; D007328:Insulin / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D007328:Insulin