title: An antisense transcript in the human cytomegalovirus UL87 gene region.
abstract: BACKGROUND
  Rapid advances in research on antisense transcripts are gradually changing our comprehension of genomic and gene expression aspects of the Herpesviridae. One such herpesvirus is the human cytomegalovirus (HCMV). Although transcription of the HCMV UL87 gene has not been specifically investigated, cDNA clones of UL87 antisense transcripts were found in HCMV cDNA libraries previously. In this study, the transcription of the UL87 antisense strand was investigated in three clinically isolated HCMV strains.
  
  
  RESULTS
  First, an 800 nucleotides transcript having an antisense orientation to the UL87 gene was found in a late HCMV cDNA library. Then, the UL87 antisense transcript was confirmed by Rapid amplification of cDNA ends (RACE) and Northern blot in three HCMV clinical strains. Two ORFs were predicted in the antisense transcript. The putative protein of ORF 1 showed a high degree of conservation among HCMV and other CMV strains.
  
  
  CONCLUSIONS
  An 800nt antisense transcript in the UL87 gene region exists in HCMV clinical strains.
authors.affiliation: Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning of China, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Mali
authors.initials: M
authors.lastname: Li
authors.forename: Shuang
authors.initials: S
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Ji
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D015152:Blotting, Northern; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D016372:RNA, Antisense / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D016372:RNA, Antisense
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