title: Identification of Escherichia coli genes associated with urinary tract infections.
abstract: Escherichia coli is the most common cause of urinary tract infections (UTIs). E. coli genes epidemiologically associated with UTIs are potentially valuable in developing strategies for treating and/or preventing such infections as well as differentiating uropathogenic E. coli from nonuropathogenic E. coli. To identify E. coli genes associated with UTIs in humans, we combined microarray-based and PCR-based analyses to investigate different E. coli source groups derived from feces of healthy humans and from patients with cystitis, pyelonephritis, or urosepsis. The cjrABC-senB gene cluster, sivH, sisA, sisB, eco274, and fbpB, were identified to be associated with UTIs. Of these, cjrABC-senB, sisA, sisB, and fbpB are known to be involved in urovirulence in the mouse model of ascending UTI. Our results provide evidence to support their roles as urovirulence factors in human UTIs. In addition, the newly identified UTI-associated genes were mainly found in members of phylogenetic groups B2 and/or D.
authors.affiliation: Institute of Molecular Medicine, National Cheng Kung University Medical College, Tainan City, Taiwan.
authors.forename: Bin-Hsu
authors.initials: BH
authors.lastname: Mao
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
authors.forename: Joy
authors.initials: J
authors.lastname: Scaria
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Chang
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Chou
authors.forename: Ni
authors.initials: N
authors.lastname: Tien
authors.forename: Jiunn-Jong
authors.initials: JJ
authors.lastname: Wu
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Tseng
authors.forename: Ming-Cheng
authors.initials: MC
authors.lastname: Wang
authors.forename: Chao-Chin
authors.initials: CC
authors.lastname: Chang
authors.forename: Yuan-Man
authors.initials: YM
authors.lastname: Hsu
authors.forename: Ching-Hao
authors.initials: CH
authors.lastname: Teng
mesh_terms: D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors
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