title: Mutations within enhancer II and BCP regions of hepatitis B virus in relation to advanced liver diseases in patients infected with subgenotype B3 in Indonesia.
abstract: Studies on the characteristics of mutations within the hepatitis B virus (HBV) genome, their roles in the pathogenesis of advanced liver diseases, and the involvement of host properties of HBV-infected individuals have not been conducted in subgenotype B3-infected populations. For addressing this issue, 40 cases with HBV surface antigen (HBsAg)-positive advanced liver diseases, including advanced liver cancer and cirrhosis (male 31, female 9, age 54.4 ± 11.6-year-old), were collected and compared with 109 cases with chronic hepatitis B (male 71, female 38, age 38.0 ± 13.4-year-old). Mutations in enhancer II (Enh II) and basal core promoter (BCP)/precore regions were analyzed by PCR-direct sequencing method. HBV viral load was examined by real-time PCR. For all examined regions, the prevalence of mutation was significantly higher in cases with advanced liver diseases. Multivariate analysis showed that, in patients older than 45 years, C1638T and T1753V mutations constituted independent risk factors for the advancement of liver diseases. The presence of C1638T and T1753V mutations may serve as predictive markers for the progression of liver diseases in Indonesia and other countries, where subgenotype B3 infection is prevalent.
authors.affiliation: Center for Infectious Diseases, Graduate School of Medicine, Kobe University, Kobe, Japan.
authors.forename: Didik Setyo
authors.initials: DS
authors.lastname: Heriyanto
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Yano
authors.forename: Takako
authors.initials: T
authors.lastname: Utsumi
authors.forename: Nungki
authors.initials: N
authors.lastname: Anggorowati
authors.forename: Hanggoro Tri
authors.initials: HT
authors.lastname: Rinonce
authors.forename: Maria Inge
authors.initials: MI
authors.lastname: Lusida
authors.lastname: Soetjipto
authors.forename: Catharina
authors.initials: C
authors.lastname: Triwikatmani
authors.forename: Neneng
authors.initials: N
authors.lastname: Ratnasari
authors.forename: Sutanto
authors.initials: S
authors.lastname: Maduseno
authors.forename: Putut Bayu
authors.initials: PB
authors.lastname: Purnama
authors.forename: Siti
authors.initials: S
authors.lastname: Nurdjanah
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology; D004742:Enhancer Elements, Genetic; D005260:Female; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000150:complications / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007214:Indonesia; D008103:Liver Cirrhosis / Q000150:complications / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load