title: Carbohydrate specificity of chicken and human tandem-repeat-type galectins-8 in composition of cells.
abstract: The network of adhesion/growth-regulatory galectins in chicken (chicken galectin, CG) has only one tandem-repeat-type protein, CG8. Using a cell-based assay and probing galectin reactivity with a panel of fluorescent neoglycoconjugates (glycoprobes), its glycan-binding profile was determined. For internal validation, human galectin-8 (HG8) was tested. In comparison to HG8, CG8 showed a rather similar specificity: both galectins displayed high affinity to blood group ABH antigens as well as to 3'-sialylated and 3'-sulfated lactosamine chains. The most remarkable difference was found to be an ability of HG8 (but not CG8) to bind the disaccharide Galβ1-3GlcNAc (Le(c)) as well as branched and linear oligolactosamines. The glycan-binding profile was shown to be influenced by glycocalix of the cell, where the galectin is anchored. Particularly, glycosidase treatment of galectin-loaded cells led to the change of the profile. Thus, we suppose the involvement of cis-glycans in the interaction of cell-anchored galectins with external glycoconjugates.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Vokhmyanina
authors.forename: E M
authors.initials: EM
authors.lastname: Rapoport
authors.forename: I M
authors.initials: IM
authors.lastname: Ryzhov
authors.forename: E Yu
authors.initials: EY
authors.lastname: Korchagina
authors.forename: G V
authors.initials: GV
authors.lastname: Pazynina
authors.forename: V V
authors.initials: VV
authors.lastname: Severov
authors.forename: H
authors.initials: H
authors.lastname: Kaltner
authors.forename: S
authors.initials: S
authors.lastname: André
authors.forename: H-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D000818:Animals; D001789:Blood Group Antigens / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D002645:Chickens; D004186:Disaccharidases / Q000737:chemistry* / Q000378:metabolism; D004285:Dogs; D037161:Galectins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry; D020080:Tandem Repeat Sequences
chemical_list: D001789:Blood Group Antigens; D037161:Galectins; C091991:LGALS8 protein, human; D011134:Polysaccharides; D004186:Disaccharidases