title: Mass spectrometry-based signal networks elucidation.
abstract: Signalling networks regulate essentially all of the biology of cells and organisms under physiological and pathological states. Analysis of signalling networks by classical biochemical approaches such as antibody-based techniques is limited for large-scale and unbiased studies. Proteomics technique based on mass spectrometry now enables the system-wide characterization of signalling events at the levels of post-translational modifications, protein complex and changes in protein expression. This function can complement the system-side gene expression analysis since the expression of many proteins is regulated by posttranscriptional mechanisms. The application of these technologies provided a quantum leap in our understanding of the molecular properties of signalling networks in recently years.
authors.affiliation: National Center of Biomedical Analysis, Beijing 100850, China. hk@proteomics.cn
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Li
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction