title: Analysis of the volatile compounds of Teucrium flavum L. subsp. flavum (Lamiaceae) by headspace solid-phase microextraction coupled to gas chromatography with flame ionisation and mass spectrometric detection.
abstract: In this study, a headspace solid-phase microextraction (HS-SPME) method, in combination with gas chromatography flame ionisation detector and gas chromatography-mass spectrometry, has been developed for use in the analysis of the volatile compounds of Teucrium flavum L. subsp. flavum, a plant whose particular fragrance is used in the preparation of flavoured wines, bitters and liqueurs, or as a substitute for hops in the flavouring of beer. The tested fibres were 100 µm poly(dimethylsiloxane) (PDMS), the 65 µm PDMS/divinylbenzene (DVB) and 50/30 µm DVB-carboxen-PDMS. The best fibre was found to be PDMS when working in the following conditions: 60°C temperature, 30 min extraction time, 30 mg sample amount, 1 mm sample particle size. The HS-SPME method permitted the identification (95.8-97.8%) of 76 (dry) and 66 (fresh) different volatiles. In addition, we discovered that the presence of water in the sample can enhance the absolute quantity of alcoholic compounds such as 1-octen-3-ol and reduce the presence of esters such as methyl geranate.
authors.affiliation: Scuola di Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, via S. Agostino 1, 62032 Camerino, Italy. gianni.sagratini@unicam.it
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.forename: Tomas
authors.initials: T
authors.lastname: Bílek
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Papa
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
mesh_terms: D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D031367:Teucrium / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000737:chemistry*
chemical_list: D055549:Volatile Organic Compounds