title: In situ synthesis of porous silica nanoparticles for covalent immobilization of enzymes.
abstract: A simple method is used to covalently encapsulate enzymes in silica nanoparticles. The encapsulation is highlighted by the high enzyme loading and porous channels that provide efficient diffusion for small substrate and product molecules while preventing protease degradation.
authors.affiliation: Institute of Nanochemistry and Nanobiology Shanghai University, Shanghai, 200444, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yang
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Zhangmei
authors.initials: Z
authors.lastname: Ye
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Haifang
authors.initials: H
authors.lastname: Wang
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Aoneng
authors.initials: A
authors.lastname: Cao
mesh_terms: D000327:Adsorption; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D012822:Silicon Dioxide; D010447:Peptide Hydrolases