title: Synthetic scaffolds increased resveratrol biosynthesis in engineered yeast cells.
abstract: Resveratrol is a polyphenolic compound produced by a few higher plants when under attack by pathogens such as bacteria or fungi. Besides antioxidant benefits to humans, this health-promoting compound has been reported to extend longevity in yeasts, flies, worms, fishes and obesity mice. Here we utilized the synthetic scaffolds strategy to improve resveratrol production in Saccharomyces cerevisiae. We observed a 5.0-fold improvement over the non-scaffolded control, and a 2.7-fold increase over the previous reported with fusion protein. This work demonstrated the synthetic scaffolds can be used for the optimization of engineered metabolic pathway.
authors.affiliation: Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO 63132, USA.
authors.forename: Yechun
authors.initials: Y
authors.lastname: Wang
authors.forename: Oliver
authors.initials: O
authors.lastname: Yu
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D054731:PDZ Domains; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013267:Stilbenes / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D011993:Recombinant Fusion Proteins; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase; D000077185:Resveratrol