title: Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis.
abstract: How kinetochores regulate microtubule dynamics to ensure proper kinetochore-microtubule interactions is unknown. Here, we studied this during early mitosis in Saccharomyces cerevisiae. When a microtubule shrinks and its plus end reaches a kinetochore bound to its lateral surface, the microtubule end attempts to tether the kinetochore. This process often fails and, responding to this failure, microtubule rescue (conversion from shrinkage to growth) occurs, preventing kinetochore detachment from the microtubule end. This rescue is promoted by Stu2 transfer (ortholog of vertebrate XMAP215/ch-TOG) from the kinetochore to the microtubule end. Meanwhile, microtubule rescue distal to the kinetochore is also promoted by Stu2, which is transported by a kinesin-8 motor Kip3 along the microtubule from the kinetochore. Microtubule extension following rescue facilitates interaction with other widely scattered kinetochores, diminishing long delays in collecting the complete set of kinetochores by microtubules. Thus, kinetochore-dependent microtubule rescue ensures efficient and sustained kinetochore-microtubule interactions in early mitosis.
authors.affiliation: Wellcome Trust Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
authors.forename: Sapan R
authors.initials: SR
authors.lastname: Gandhi
authors.forename: Marek
authors.initials: M
authors.lastname: Gierliński
authors.forename: Akihisa
authors.initials: A
authors.lastname: Mino
authors.forename: Kozo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Etsushi
authors.initials: E
authors.lastname: Kitamura
authors.forename: Lesley
authors.initials: L
authors.lastname: Clayton
authors.forename: Tomoyuki U
authors.initials: TU
authors.lastname: Tanaka
mesh_terms: D002478:Cells, Cultured; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; C109414:STU2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
references.citation: Annu Rev Biophys. 2008;37:465-87
references.pmid: 18573091
references.citation: Genes Dev. 2007 Dec 15;21(24):3319-30
references.pmid: 18079178
references.citation: Biochim Biophys Acta. 2000 Mar 17;1496(1):99-116
references.pmid: 10722880
references.citation: J Cell Sci. 2001 Nov;114(Pt 21):3805-12
references.pmid: 11719547
references.citation: Nat Cell Biol. 2006 Sep;8(9):957-62
references.pmid: 16906145
references.citation: J Cell Sci. 2008 Dec 15;121(Pt 24):4114-23
references.pmid: 19033380
references.citation: Cell. 2008 Jan 11;132(1):79-88
references.pmid: 18191222
references.citation: EMBO J. 2005 Apr 6;24(7):1387-96
references.pmid: 15775959
references.citation: J Cell Biol. 1990 Jan;110(1):81-95
references.pmid: 2295685
references.citation: Cell. 2009 Sep 18;138(6):1174-83
references.pmid: 19766569
references.citation: Nat Cell Biol. 2006 Sep;8(9):913-23
references.pmid: 16906148
references.citation: J Cell Biol. 2001 Apr 16;153(2):435-42
references.pmid: 11309422
references.citation: J Cell Biol. 1997 Dec 1;139(5):1271-80
references.pmid: 9382872
references.citation: Nature. 2005 Nov 17;438(7066):384-8
references.pmid: 16292315
references.citation: J Cell Biol. 2007 Jul 16;178(2):269-81
references.pmid: 17620411
references.citation: EMBO J. 2001 Jul 2;20(13):3389-401
references.pmid: 11432827
references.citation: Int Rev Cytol. 2004;239:179-272
references.pmid: 15464854
references.citation: Nat Cell Biol. 2003 Sep;5(9):812-8
references.pmid: 12894167
references.citation: Curr Opin Cell Biol. 2010 Feb;22(1):57-67
references.pmid: 20061128
references.citation: Mol Biol Cell. 2007 Aug;18(8):3094-104
references.pmid: 17553931
references.citation: Nat Cell Biol. 2001 Jan;3(1):E23-7
references.pmid: 11146646
references.citation: Dev Cell. 2010 Feb 16;18(2):248-59
references.pmid: 20159595
references.citation: Curr Biol. 2011 Feb 8;21(3):207-13
references.pmid: 21256019
references.citation: J Cell Biol. 2006 Mar 27;172(7):1009-22
references.pmid: 16567500
references.citation: Nat Rev Mol Cell Biol. 2008 Apr;9(4):309-22
references.pmid: 18322465
references.citation: J Biol Chem. 2000 Jul 7;275(27):20748-53
references.pmid: 10770946
references.citation: Mol Biol Cell. 2006 Jun;17(6):2789-98
references.pmid: 16571681
references.citation: Dev Cell. 2004 Jun;6(6):815-29
references.pmid: 15177030
references.citation: Genes Cells. 2002 Aug;7(8):781-9
references.pmid: 12167157
references.citation: Nature. 2005 Apr 21;434(7036):987-94
references.pmid: 15846338
references.citation: Dev Cell. 2004 Jun;6(6):831-43
references.pmid: 15177031
references.citation: Curr Biol. 2011 Feb 8;21(3):214-20
references.pmid: 21256022
references.citation: Curr Biol. 2001 Apr 17;11(8):537-49
references.pmid: 11369198
references.citation: EMBO J. 2010 Dec 15;29(24):4070-82
references.pmid: 21102558