title: Induction of antigen-specific immunity by pH-sensitive carbonate apatite as a potent vaccine carrier.
abstract: The ability of carbonate apatite (CO(3)Ap) to enhance antigen-specific immunity was examined in vitro and in vivo to investigate its utility as a vaccine carrier. Murine bone marrow-derived dendritic cells took up ovalbumin (OVA) containing CO(3)Ap more effectively than free OVA. Interestingly, mice immunized with OVA-containing CO(3)Ap produced OVA-specific antibodies more effectively than mice immunized with free OVA. Furthermore, immunization of C57BL/6 mice with OVA-containing CO(3)Ap induced the proliferation and antigen-specific production of IFN-γ by splenocytes more strongly than immunization with free OVA. Moreover, no significant differences were detected in the induction of delayed-type hypersensitivity responses, an immune reaction involving an antigen-specific, cell-mediated immune response between OVA-containing CO(3)Ap and OVA-containing alumina salt (Alum), suggesting that CO(3)Ap induced cell-mediated immune response to the same degree as Alum, which is commonly used for clinical applications. This study is the first to demonstrate the induction of antigen-specific immune responses in vivo by CO(3)Ap.
authors.affiliation: Viral Infectious Diseases Unit, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Hebishima
authors.forename: Seiichi
authors.initials: S
authors.lastname: Tada
authors.forename: Shin-nosuke
authors.initials: SN
authors.lastname: Takeshima
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Yoko
authors.initials: Y
authors.lastname: Aida
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D001031:Apatites / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D006863:Hydrogen-Ion Concentration; D007111:Immunity, Cellular; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D000941:Antigens; D001031:Apatites; D022223:Vaccines, Subunit; C030782:carboapatite; D010047:Ovalbumin