title: Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects.
abstract: Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA(2) was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA(2)s of viperid snake venoms. The Drs-PLA(2) had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC(50) of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC(50) of 25.6nM. In addition, the Drs-PLA(2) inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%.
authors.forename: Suchitra
authors.initials: S
authors.lastname: Khunsap
authors.forename: Narumol
authors.initials: N
authors.lastname: Pakmanee
authors.forename: Orawan
authors.initials: O
authors.lastname: Khow
authors.forename: Lawan
authors.initials: L
authors.lastname: Chanhome
authors.forename: Visith
authors.initials: V
authors.lastname: Sitprija
authors.forename: Montamas
authors.initials: M
authors.lastname: Suntravat
authors.forename: Sara E
authors.initials: SE
authors.lastname: Lucena
authors.forename: John C
authors.initials: JC
authors.lastname: Perez
authors.forename: Elda E
authors.initials: EE
authors.lastname: Sánchez
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