title: The offline combination of thin-layer chromatography and high-performance liquid chromatography with diode array detection and micrOTOF-Q mass spectrometry for the separation and identification of spinochromes from sea urchin (Strongylocentrotus droebachiensis) shells.
abstract: Thin-layer chromatography (TLC) with off-line high-performance liquid chromatography coupled to diode array detection and micrOTOF-Q mass spectrometry (HPLC-DAD-MS) resulted in the successful fractionation, separation and identification of spinochrome pigments from sea urchin (Strongylocentrotus droebachiensis) shells. Two fractions of pigments were separated by TLC and eluted with methanol using a TLC-MS interface. HPLC-DAD-MS analysis of the fractions indicated the presence of six sea urchin pigments: spinochrome monomers B and D, three spinochrome dimers (anhydroethylidene-6,6'-bis(2,3,7-trihydroxynaphthazarin) and its isomer and ethylidene-6,6'-bis(2,3,7-trihydroxynaphthazarin)), and one pigment that was preliminary identified as a spinochrome dimer with the structural formula C(22)H(16)O(16).
authors.affiliation: Saint-Petersburg Institute of Pharmacy, 47/33 Piskarevsky pr., St.-Petersburg, Russia. alexs79@mail.ru
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shikov
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Ossipov
authors.forename: Olli
authors.initials: O
authors.lastname: Martiskainen
authors.forename: Olga N
authors.initials: ON
authors.lastname: Pozharitskaya
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Ivanova
authors.forename: Valery G
authors.initials: VG
authors.lastname: Makarov
mesh_terms: D060105:Animal Shells / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification*; D010860:Pigments, Biological / Q000737:chemistry / Q000302:isolation & purification*; D047269:Strongylocentrotus / Q000737:chemistry*
chemical_list: D009285:Naphthoquinones; D010860:Pigments, Biological; C009304:spinochrome A