title: Age-related decrease of miRNA-92a levels in human CD8+ T-cells correlates with a reduction of naïve T lymphocytes.
abstract: MicroRNA (miR)-17-92a expression plays a crucial role in lymphocyte ontogeny. We therefore set out to determine miR-92a expression levels in peripheral blood lymphocytes from healthy subjects to ascertain any association between these levels and ageing. We found a positive correlation between the miR-92a expression level and the percentages of RO-CD8+CD27+ (P = 0.0046) and CD3+CD8+CD62L+ (P = 0.0011). This suggests that the majority of miR-92a of CD8+ T cells is derived from naïve cells, and the miR-92a expression level in CD8+ T cells declines progressively with age. These results indicate that the age-related attrition of naïve T cells is linked to a reduction of miR-92a in human T -lymphocytes. Therefore, we should careful attention when evaluating human miRNA levels in T lymphocytes to use normal control values.
authors.affiliation: Department of Hematology, Tokyo Medical University, Tokyo, Japan. ohyashik@rr.iij4u.or.jp.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Ohyashiki
authors.forename: Junko H
authors.initials: JH
authors.lastname: Ohyashiki
authors.forename: Ayako
authors.initials: A
authors.lastname: Hirota
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kobayashi
authors.forename: Kazuma
authors.initials: K
authors.lastname: Ohyashiki
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