title: A cytotoxic 4α-methyl steroid from the aerial parts of Cimicifuga foetida L.
abstract: A new 4α-methyl sterol, cimisterol A (1), together with five known compounds (2-6), were isolated from the aerial parts of Cimicifuga foetida L. The new compound's structure was determined with the help of extensive 1D and 2D NMR spectroscopy. Compound 1 exhibited broad-spectrum and potent cytotoxic activities against human HL-60, Jurkat, K562, U937, HepG-2, and SGC-7091 cell lines, with IC(50) values of 7.23, 2.89, 6.88, 3.38, 4.21, and 4.89 μM, respectively. Compound 3 showed moderate to weak activities to all cell lines, except for SGC-7091, having IC(50) values ranging from 13.37 to 17.72 μM. This is the first time a cytotoxic 4α-methyl sterol constituent was discovered from Cimicifuga spp.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Nian
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Su
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Qiu
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D002783:Cholestenones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031885:Cimicifuga / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C579547:3,21-dihydroxy-4-methyl-5,14-ergosta-8,24(28)-dien-7-one; D002783:Cholestenones; D013256:Steroids