title: In-cell click labelling of small molecules to determine subcellular localisation.
abstract: Small molecule fluorometric boron dipyrromethene probes were developed to bind hepatitis C virus-encoded NS5A protein and aid subcellular distribution studies. These molecules did not co-locate with NS5A, therefore alternative 'silent' azide reporters were used to obtain a more relevant picture of their distribution. Following pre-incubation with replicon cells, click chemistry was used to append a fluorophore to the azide that confirmed the co-localisation of the small molecule with the NS5A protein, thus providing greater insight into the antiviral mode of action of this chemotype.
  
  
  BACKGROUND
  The online version of this article (doi:10.1007/s12154-010-0047-1) contains supplementary material, which is available to authorized users.
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