title: Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF.
abstract: In epigenetic signaling pathways, histone tails are heavily modified, resulting in the recruitment of effector molecules that can influence transcription. One such molecule, plant homeodomain finger protein 20 (PHF20), uses a Tudor domain to read dimethyl lysine residues and is a known component of the MOF (male absent on the first) histone acetyltransferase protein complex, suggesting it plays a role in the cross-talk between lysine methylation and histone acetylation. We sought to investigate the biological role of PHF20 by generating a knockout mouse. Without PHF20, mice die shortly after birth and display a wide variety of phenotypes within the skeletal and hematopoietic systems. Mechanistically, PHF20 is not required for maintaining the global H4K16 acetylation levels or locus specific histone acetylation but instead works downstream in transcriptional regulation of MOF target genes.
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Aimee I
authors.initials: AI
authors.lastname: Badeaux
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Yanzhong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Kim
authors.initials: K
authors.lastname: Cardenas
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Vidyasiri
authors.initials: V
authors.lastname: Vemulapalli
authors.affiliation: Division of Biostatistics, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: Kaifu
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Donna
authors.initials: D
authors.lastname: Kusewitt
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957.
authors.forename: Ellen
authors.initials: E
authors.lastname: Richie
authors.affiliation: Division of Biostatistics, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Molecular Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957. Electronic address: mtbedford@mdanderson.org.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Bedford
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D055786:Gene Knockout Techniques; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008297:Male; D051379:Mice; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D018398:Homeodomain Proteins; C571905:PHF20 protein, mouse; D014157:Transcription Factors; D051548:Histone Acetyltransferases; C000622683:Kat8 protein, mouse; D008239:Lysine
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