title: [Differentially expressed urine protein of contrast induced acute kidney injury by two-dimensional differential in-gel electrophoresis and mass spectrometry].
abstract: OBJECTIVE
  To explore the differentially expressed proteins of contrast-induced nephropathy through a comparison of urinary proteome so as to further elucidate the pathogenesis and discover the disease biomarker.
  
  
  METHODS
  The urine samples of 12 patients were collected before and after coronary angiography. Two dimensional electrophoresis was performed after the urine samples were labeled by different dyes. The differences of urine proteome were analyzed by Decyder software and the differentially expressed spots identified by mass spectrometry.
  
  
  RESULTS
  A total of 56 differentially expressed spots were detected. Among them, 39 spots were up-regulated and 17 spots down-regulated. And mannose binding lectin and mannose binding lectin associated serine protease 2, key proteins in complement body activation, were both significantly up-regulated.
  
  
  CONCLUSIONS
  Urine proteomic study methods are constructed based on 2D-DIGE and mass spectrometry. The lectin pathway of complement body may be associated with contrast-induced acute kidney injury.
authors.affiliation: Department of Nephrology, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Ni
authors.forename: Zhen-sheng
authors.initials: ZS
authors.lastname: Xie
authors.forename: Fu-quan
authors.initials: FQ
authors.lastname: Yang
authors.forename: Ben
authors.initials: B
authors.lastname: He
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Liu
authors.forename: Hui-li
authors.initials: HL
authors.lastname: Dai
authors.forename: Yu-cheng
authors.initials: YC
authors.lastname: Yan
authors.forename: Jia-qi
authors.initials: JQ
authors.lastname: Qian
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D011506:Proteins / Q000032:analysis; D011507:Proteinuria / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D011506:Proteins; D020543:Proteome