title: Alcohol-based quorum sensing plays a role in adhesion and sliding motility of the yeast Debaryomyces hansenii.
abstract: The yeast Debaryomyces hansenii was investigated for its production of alcohol-based quorum sensing (QS) molecules including the aromatic alcohols phenylethanol, tyrosol, tryptophol and the aliphatic alcohol farnesol. Debaryomyces hansenii produced phenylethanol and tyrosol, which were primarily detected from the end of exponential phase indicating that they are potential QS molecules in D. hansenii as previously shown for other yeast species. Yields of phenylethanol and tyrosol produced by D. hansenii were, however, lower than those produced by Candida albicans and Saccharomyces cerevisiae and varied with growth conditions such as the availability of aromatic amino acids, ammonium sulphate, NaCl, pH and temperature. Tryptophol was only produced in the presence of tryptophane, whereas farnesol in general was not detectable. Especially, the type strain of D. hansenii (CBS767) had good adhesion and sliding motility abilities, which seemed to be related to a higher hydrophobicity of the cell surface of D. hansenii (CBS767) rather than the ability to form pseudomycelium. Addition of phenylethanol, tyrosol, tryptophol and farnesol was found to influence both adhesion and sliding motility of D. hansenii.
authors.affiliation: Department of Food Science, Food Microbiology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Klaus
authors.initials: K
authors.lastname: Gori
authors.forename: Peter B
authors.initials: PB
authors.lastname: Knudsen
authors.forename: Kristian F
authors.initials: KF
authors.lastname: Nielsen
authors.forename: Nils
authors.initials: N
authors.lastname: Arneborg
authors.forename: Lene
authors.initials: L
authors.lastname: Jespersen
mesh_terms: D000438:Alcohols / Q000302:isolation & purification / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002448:Cell Adhesion / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D055165:Debaryomyces / Q000254:growth & development / Q000502:physiology*; D005204:Farnesol / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000302:isolation & purification / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism; D011137:Polystyrenes; D053038:Quorum Sensing / Q000502:physiology*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D000438:Alcohols; D007211:Indoles; D011137:Polystyrenes; C011867:4-hydroxyphenylethanol; D005204:Farnesol; C005949:tryptophol; D010626:Phenylethyl Alcohol