title: Identification and functional characterization of polo-like kinase 2 autoregulatory sites.
abstract: Polo family kinases play important roles in cellular proliferation as well as neuronal synaptic plasticity. However, the posttranslational regulation of these kinases is not fully understood. Here, we identified several novel Plk2 phosphorylation sites stimulated by Plk2 itself. By site-directed mutagenesis, we uncovered three additional hyperactivating Plk2 mutations as well as a series of residues regulating Plk2 steady-state expression level. Because of the established role of Plk2 in homeostatic negative control of excitatory synaptic strength, these phosphorylation sites could play an important role in the rapid activation, expansion, and prolongation of Plk2 signaling in this process.
authors.affiliation: Department of Pharmacology and Physiology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057-1464, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Rozeboom
authors.forename: D T S
authors.initials: DT
authors.lastname: Pak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D020134:Catalytic Domain / Q000235:genetics; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006706:Homeostasis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014162:Transfection
chemical_list: D004247:DNA; C496126:PLK2 protein, human; D017346:Protein Serine-Threonine Kinases
references.citation: Cell. 2003 Oct 3;115(1):83-95
references.pmid: 14532005
references.citation: Trends Biochem Sci. 1996 Jul;21(7):267-71
references.pmid: 8755249
references.citation: J Biol Chem. 2002 Nov 15;277(46):44115-20
references.pmid: 12207013
references.citation: Oncogene. 2005 Jan 10;24(2):248-59
references.pmid: 15640840
references.citation: J Biol Chem. 2010 Jan 22;285(4):2807-22
references.pmid: 19889641
references.citation: J Biol Chem. 2004 Feb 13;279(7):5405-12
references.pmid: 14645242
references.citation: Mol Cell Biol. 1999 Dec;19(12):8625-32
references.pmid: 10567586
references.citation: FASEB J. 1987 Nov;1(5):349-57
references.pmid: 2824267
references.citation: Mol Cell Biol. 1992 Sep;12(9):4164-9
references.pmid: 1508211
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Biochemistry. 2007 Aug 21;46(33):9551-63
references.pmid: 17655330
references.citation: J Biol Chem. 2009 Nov 13;284(46):32053-65
references.pmid: 19700763
references.citation: J Cell Biol. 1995 Jun;129(6):1617-28
references.pmid: 7790358
references.citation: Science. 2009 Sep 25;325(5948):1682-6
references.pmid: 19779198
references.citation: EMBO J. 2001 May 15;20(10):2367-75
references.pmid: 11350925
references.citation: Oncogene. 2005 Jan 10;24(2):292-8
references.pmid: 15640845
references.citation: Science. 2003 Nov 21;302(5649):1368-73
references.pmid: 14576440
references.citation: PLoS One. 2008 Apr 23;3(4):e1997
references.pmid: 18431482
references.citation: J Biol Chem. 2004 May 14;279(20):20775-85
references.pmid: 14988399
references.citation: Neuron. 2011 Mar 10;69(5):957-73
references.pmid: 21382555
references.citation: Mol Cell Biol. 2005 Mar;25(5):2031-44
references.pmid: 15713655
references.citation: Curr Biol. 1998 Apr 23;8(9):497-507
references.pmid: 9560342
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1847-52
references.pmid: 17264206
references.citation: Neuron. 2008 May 22;58(4):571-83
references.pmid: 18498738
references.citation: Oncogene. 2008 Aug 21;27(36):4900-8
references.pmid: 18427546
references.citation: J Biol Chem. 2002 Jul 12;277(28):25247-56
references.pmid: 11994303
references.citation: J Biol Chem. 1997 Apr 4;272(14):9166-74
references.pmid: 9083047
references.citation: J Biol Chem. 2009 Jan 30;284(5):2598-2602
references.pmid: 19004816
references.citation: Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1984-9
references.pmid: 11854496
references.citation: Mol Cell. 1998 Aug;2(2):163-71
references.pmid: 9734353
references.citation: EMBO J. 1998 Mar 2;17(5):1336-49
references.pmid: 9482731
references.citation: Mol Cell. 2007 Dec 14;28(5):730-8
references.pmid: 18082598
references.citation: Science. 2003 Feb 21;299(5610):1228-31
references.pmid: 12595692
references.citation: Neuron. 2001 Aug 2;31(2):289-303
references.pmid: 11502259
references.citation: Nat Rev Mol Cell Biol. 2009 Apr;10(4):265-75
references.pmid: 19305416
references.citation: EMBO J. 1999 Oct 15;18(20):5528-39
references.pmid: 10523297