title: Expression of the human tumor suppressor p53 induces cell death in Pichia pastoris.
abstract: The human tumor suppressor p53 is known as guardian of genome because of its involvement in many signals related to cell life or death. In this work, we report that human p53 induces cell death in the yeast Pichia pastoris. We showed a growth inhibition effect, which increased with the p53 protein expression level in recombinant Mut(s)  (methanol utilization slow) strain of Pichia. However, no effect of p53 was observed in recombinant strain of Mut(+)  (methanol utilization plus) phenotype. Interestingly, human p53 induces cell death in recombinant strains Mut(s)  with characteristic markers of apoptosis such as DNA fragmentation, exposure of phosphatidylserine, and reactive oxygen species generation. Taken together, our results strongly suggest that human p53 is biologically active in this heterologous context. Thus, we propose that P. pastoris could be a useful tool to better understand the biological function of human p53.
authors.affiliation: Laboratoire de Valorisation de la Biomasse et Production de Protéines chez les Eucaryotes, Centre de Biotechnologie de Sfax, University of Sfax, Sfax, Tunisia.
authors.forename: Salma
authors.initials: S
authors.lastname: Abdelmoula-Souissi
authors.forename: Imed
authors.initials: I
authors.lastname: Mabrouk
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.forename: Raja
authors.initials: R
authors.lastname: Mokdad-Gargouri
mesh_terms: D017209:Apoptosis; D053938:DNA Fragmentation; D015870:Gene Expression; D006801:Humans; D010718:Phosphatidylserines / Q000032:analysis; D010843:Pichia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D010718:Phosphatidylserines; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53