title: Experimental antibacterial and mineralizing calcium phosphate-based treatment for dentin surfaces.
abstract: OBJECTIVE
  This study aimed to determine the efficacy of experimental calcium phosphate-based solutions (sCaP) containing fluoride (F), with and without zinc (Zn) ions on reducing susceptibility to acid dissolution and Streptococcus mutans (S. mutans) colonization of dentin surfaces.
  
  
  METHODS
  Dentin sections were treated with double distilled water (control) and with sCaP solutions differing in pH and in F(-) and/or Zn(2+) ion concentrations. Solutions A (pH 7); B, C, and D (pH 5.5); solution C, twice Zn(2+) and F(-) ion concentration compared to B; solution D is similar to C but without Zn(2+). The dentin surfaces were characterized using scanning electron microscopy (SEM), x-ray diffraction, and Fourier Transform Infrared spectroscopy. Dissolution was determined in acidic buffer. Bacterial (S. mutans) attachment and growth were evaluated using SEM and Bioquant. Statistical analyses applied analysis of variance (ANOVA) and Duncan's multiple Range test.
  
  
  RESULTS
  Compared to control, dentin surfaces treated with sCaP solutions showed: (a) occluded dentin tubules; (b)reduced susceptibility to acid dissolution; and (c) Zn(2+) ions were more effective than F(-) ions in inhibiting bacterial colonization.
  
  
  CONCLUSIONS
  Acidic sCaP containing both F and Zn ions have mineralizing, acid resistance, and antibacterial effects and may be potentially useful as a strategy against dentin caries formation and progression.
authors.affiliation: Guanghua School of Stomatology, Sun Yat-sen University, 56 Ling Yuan Xi Road, Guangzhou 510055, China.
authors.forename: Haijing
authors.initials: H
authors.lastname: Gu
authors.forename: Dindo
authors.initials: D
authors.lastname: Mijares
authors.forename: Ziming
authors.initials: Z
authors.lastname: Zhao
authors.forename: Robert
authors.initials: R
authors.lastname: Boylan
authors.forename: Junqi
authors.initials: J
authors.lastname: Ling
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: LeGeros
mesh_terms: D000143:Acids / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects* / Q000382:microbiology / Q000648:ultrastructure; D005459:Fluorides / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development; D013499:Surface Properties / Q000187:drug effects; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000143:Acids; D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; C020243:calcium phosphate; D015032:Zinc; D005459:Fluorides