title: Detection of bacterial DNA in bile of cats with lymphocytic cholangitis.
abstract: In this study, we have successfully used molecular methods based on the amplification of the 16S ribosomal RNA gene on feline bile samples to show that bile of cats with LC is not sterile. This is probably due to the fact that the inflammatory process in the biliary tree causes dilatations. As a result, bacteria can easily migrate from the intestines via the common bile duct. The diversity of species identified and the presence of Helicobacter spp. DNA in both patients and controls suggests that bacteriobilia is secondary to the disease and is not the cause of LC.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. cmaotte@hotmail.com
authors.forename: C M A
authors.initials: CM
authors.lastname: Otte
authors.forename: O Pérez
authors.initials: OP
authors.lastname: Gutiérrez
authors.forename: R P
authors.initials: RP
authors.lastname: Favier
authors.forename: J
authors.initials: J
authors.lastname: Rothuizen
authors.forename: L C
authors.initials: LC
authors.lastname: Penning
mesh_terms: D000818:Animals; D001646:Bile / Q000382:microbiology*; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D002761:Cholangitis / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D005260:Female; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S