title: Self-microemulsifying smaller molecular volume oil (Capmul MCM) using non-ionic surfactants: a delivery system for poorly water-soluble drug.
abstract: The main purpose of this work is to formulate self-microemulsifying drug delivery system (SMEDDS) using smaller molecular oil with Atorvastatin calcium as a model drug. Solubility of the selected drug was accessed in oils and surfactants. Percent transmittance (%T) test study was performed to identify the efficient self-microemulsifying formulations. Those formulations which showed higher value for %T were evaluated for droplet size, polydispersity index, ζ potential, refractive index and cloud point measurement. Effect of drug loading on droplet size, increasing dilution in different media, thermodynamic stability and in vitro dissolution was performed to observe the performance of the selected formulation. Further cytotoxicity and permeation enhancement studies were carried out on Caco2 cell lines. Of all the oils accessed for drug solubility, Capmul MCM showed higher solubility capacity for Atorvastatin calcium. Capmul MCM was better microemulsified using combination of Tween 20 and Labrasol surfactant. Droplet size was as low as 86.93 nm with polydispersity index and ζ potential at 0.195 ± 0.011 and -7.27 ± 3.11 mV respectively. The selected undiluted formulation showed refractive index values ranging from 1.40 to 1.47 indicating the isotropicity of the formulation. The selected formulation was robust to dilution in different media and thermodynamically stable. Dissolution profile was enhanced for the selected drug as compared to marketed formulation with t85% and DE values at 10 min and 80.15 respectively. Also cytotoxicity measurement showed minimum effect with good permeation enhancing capacity. Thus our study demonstrates the use of smaller molecular oil (Capmul MCM) for developing self-microemulsifying drug delivery system for better in vitro and in vivo performance.
authors.affiliation: Department of Pharmaceutics, AISSMS College of Pharmacy, Kennedy Road, Near RTO, Pune, Maharashtra, India. mithunband@rediffmail.com
authors.forename: Mithun Mohanraor
authors.initials: MM
authors.lastname: Bandivadeka
authors.forename: Shyam Sundar
authors.initials: SS
authors.lastname: Pancholi
authors.forename: Ruchika
authors.initials: R
authors.lastname: Kaul-Ghanekar
authors.forename: Amit
authors.initials: A
authors.lastname: Choudhari
authors.forename: Soumya
authors.initials: S
authors.lastname: Koppikar
mesh_terms: D000069059:Atorvastatin; D018938:Caco-2 Cells; D002210:Caprylates / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D016503:Drug Delivery Systems; D004655:Emulsions; D005989:Glycerides / Q000737:chemistry*; D006538:Heptanoic Acids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D009821:Oils / Q000737:chemistry; D009930:Organic Chemicals / Q000737:chemistry; D010316:Particle Size; D010539:Permeability; D011136:Polysorbates / Q000737:chemistry; D011758:Pyrroles / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D002210:Caprylates; D004655:Emulsions; D005989:Glycerides; D006538:Heptanoic Acids; D009821:Oils; D009930:Organic Chemicals; D011136:Polysorbates; D011758:Pyrroles; D013501:Surface-Active Agents; C440220:Labrasol; D000069059:Atorvastatin; C025343:monooctanoin