title: The solid-state characterization of fusidic acid.
abstract: OBJECTIVE
  The aim of this work was to characterize the solid-state properties of fusidic acid (FA).
  
  
  METHODS
  Solid forms of FA were prepared by solvent-mediated polymorphic transformation of commercial FA (Form III) in acetonitrile (ACN), and methanol:H(2)O (50:50), or generated by solvent recrystallization from dichloromethane (DCM). Polymorphs were characterized using, X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), polarizing hot stage microscopy (HSM), and intrinsic dissolution rate (IDR).
  
  
  RESULTS
  Slurrying commercial FA (Form III) in methanol:H(2)O (50:50), yielded a metastable form (Form IV). This metastable form converts to Form I or back to Form III in ACN and H(2)O, respectively, and Form II upon recrystallization from DCM. IDR of Form IV was 0.092 mg/min/cm(2), and was statistically different (p<0.05) from the IDR of Forms I, II, and III, with IDR of 0.053, 0.043, and 0.045mg/min/cm(2), respectively. The amorphous FA had an IDR of 0.125 mg/min/cm(2), and was significantly higher (p<0.05) than any other solid form. There were no statistical differences in the IDR of Form I, II, or III.
  
  
  CONCLUSIONS
  This work provides evidence for the existence of two previously unreported polymorphic forms of FA (Forms II and IV) and an amorphate.
authors.affiliation: Faculty of Pharmaceutical Science, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Gilchrist
authors.forename: Kevin
authors.initials: K
authors.lastname: Letchford
authors.forename: Helen M
authors.initials: HM
authors.lastname: Burt
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical; D003460:Crystallization; D018360:Crystallography, X-Ray; D005672:Fusidic Acid / Q000737:chemistry*; D006358:Hot Temperature; D007700:Kinetics; D000432:Methanol / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D008859:Microscopy, Polarization; D008958:Models, Molecular; D033362:Powder Diffraction; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013818:Thermogravimetry
chemical_list: D000097:Acetonitriles; D012997:Solvents; D008752:Methylene Chloride; D005672:Fusidic Acid; D000432:Methanol; C032159:acetonitrile