title: Two-temperature hybridization for microarray detection of label-free microRNAs with attomole detection and superior specificity.
abstract: Two is better than one: Two short locked nucleic acid based probes were used to collectively capture and detect microRNAs by a simple two-temperature hybridization process. Intact microRNAs were directly measured down to attomolar concentrations with a high specificity and nearly four orders of magnitude of dynamic range. Single base mismatches in the microRNAs were potently discriminated from the perfectly matched targets.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-806, Korea.
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Yongwon
authors.initials: Y
authors.lastname: Jung
mesh_terms: D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013696:Temperature
chemical_list: D035683:MicroRNAs