title: Queen volatiles as a modulator of Tetragonisca angustula drone behavior.
abstract: Tetragonisca angustula mating occurs during the virgin queen nuptial flight, usually in the presence of a drone congregation area (DCA). The presence of virgin queen pheromone is considered the trigger for DCA establishment, although this has not been demonstrated experimentally. We established meliponaries, in different habitats, with T. angustula virgin queens during the main drone reproduction period. Eight DCAs were observed in urban areas, and all established outside or near colonies containing at least one virgin queen. The accumulation of drones in the DCAs occurred from 08:00 to 18:00 h and over 3-35 days. The number of drones in DCAs ranged from 60 to 2,000. In field trials, drones were attracted to virgin queens and also, unexpectedly, to physogastric queens. Volatiles collected from both virgin and physogastric queens elicited strong electoantennogram (EAG) responses from drones. Virgin and physogastric queen volatiles were qualitatively similar, but quantitatively different, in chemical composition. The queen's abdomen was the principal source of these compounds. Isopropyl hexanoate (IPH), the most abundant compound in virgin queen volatiles and one of the most abundant in physogastric queen volatiles, was identified as one of the compounds that elicited EAG responses and was demonstrated to attract drones in a field test.
authors.affiliation: El Colegio de la Frontera Sur, Unidad Tapachula, Tapachula, Chiapas, Mexico.
authors.forename: Macario M
authors.initials: MM
authors.lastname: Fierro
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Cruz-López
authors.forename: Daniel
authors.initials: D
authors.lastname: Sánchez
authors.forename: Rogel
authors.initials: R
authors.lastname: Villanueva-Gutiérrez
authors.forename: Remy
authors.initials: R
authors.lastname: Vandame
mesh_terms: D000818:Animals; D001516:Bees / Q000737:chemistry* / Q000187:drug effects* / Q000502:physiology; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005260:Female; D008297:Male; D012746:Sexual Abstinence; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D055549:Volatile Organic Compounds / Q000032:analysis / Q000494:pharmacology*
chemical_list: D055549:Volatile Organic Compounds
references.citation: J Chem Ecol. 2006 Feb;32(2):453-71
references.pmid: 16555129
references.citation: Neotrop Entomol. 2012 Apr;41(2):95-104
references.pmid: 23950022
references.citation: Anim Behav. 1972 Feb;20(1):123-32
references.pmid: 4664920
references.citation: Genetics. 1977 Jul;86(3):583-96
references.pmid: 892423