title: Changes of microRNAs-192, 196a and 203 correlate with Barrett's esophagus diagnosis and its progression compared to normal healthy individuals.
abstract: BACKGROUND
  Barrett's esophagus (BE) is a disease with a rising prevalence in western countries probably due to the unhealthy lifestyle. In significant number of cases it develops to esophageal adenocarcinoma. Two decades ago, important gene regulators (microRNAs) were discovered and their attendance in the process of malignant transformation was demonstrated (e.g. miR-192, 196a, 203). Our aim was to select the patients with the increased risk of malignant transformation before the cancer develops.
  
  
  METHODS
  71 patients with BE disease were selected, slides from FFPE blocks were prepared, the lesions were microdissected and a qPCR relative expression analysis for selected microRNAs (generally known to be connected with malignant transformation process) was carried out.
  
  
  RESULTS
  We demonstrated unequivocal statistically significant upregulation of two microRNAs (miR-192, 196a) and downregulation of miR-203 and positive miR-196a correlation with progression from intestinal metaplasia to adenocarcinoma compared to normal individuals.
  
  
  CONCLUSIONS
  We hypothesize that there do exist changes of selected microRNAs which can undoubtedly distinguish the patients with BE from normal healthy individuals.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Hnevotinska 3, Olomouc, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Luzna
authors.forename: Jan
authors.initials: J
authors.lastname: Gregar
authors.forename: Ivo
authors.initials: I
authors.lastname: Uberall
authors.forename: Lenka
authors.initials: L
authors.lastname: Radova
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Prochazka
authors.forename: Jiri
authors.initials: J
authors.lastname: Ehrmann
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000209:etiology / Q000235:genetics; D001471:Barrett Esophagus / Q000150:complications / Q000175:diagnosis* / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D018450:Disease Progression; D015536:Down-Regulation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007422:Intestines / Q000473:pathology*; D061346:Laser Capture Microdissection; D008679:Metaplasia / Q000175:diagnosis / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D012189:Retrospective Studies; D012307:Risk Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D015415:Biomarkers; C546783:MIRN192 microRNA, human; C542543:MIRN196 microRNA, human; C546703:MIRN203 microRNA, human; D035683:MicroRNAs
references.citation: Genome Biol. 2009;10(6):R64
references.pmid: 19531210
references.citation: Semin Radiat Oncol. 2007 Jan;17(1):2-9
references.pmid: 17185192
references.citation: Am J Pathol. 2009 May;174(5):1940-8
references.pmid: 19342367
references.citation: Clin Cancer Res. 2009 Sep 15;15(18):5744-52
references.pmid: 19737949
references.citation: Virchows Arch. 2008 Jan;452(1):1-10
references.pmid: 18040713
references.citation: N Engl J Med. 1976 Aug 26;295(9):476-80
references.pmid: 940579
references.citation: Nucleic Acids Res. 2001 May 1;29(9):e45
references.pmid: 11328886
references.citation: J Thorac Cardiovasc Surg. 2008 Feb;135(2):255-60; discussion 260
references.pmid: 18242245
references.citation: RNA Biol. 2011 Sep-Oct;8(5):706-13
references.pmid: 21712654
references.citation: Gastroenterology. 2010 Mar;138(3):854-69
references.pmid: 20080098
references.citation: Clin Cancer Res. 2011 Aug 15;17(16):5287-98
references.pmid: 21159887
references.citation: Nat Rev Cancer. 2010 Feb;10(2):87-101
references.pmid: 20094044
references.citation: J Thorac Cardiovasc Surg. 1963 Jan;45:13-34
references.pmid: 14011100
references.citation: J Mol Diagn. 2003 Feb;5(1):34-41
references.pmid: 12552078
references.citation: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references.pmid: 12184808
references.citation: Am J Pathol. 2001 Feb;158(2):419-29
references.pmid: 11159180
references.citation: Clin Chem. 2008 Oct;54(10):1716-24
references.pmid: 18719196
references.citation: Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999-3004
references.pmid: 14973191
references.citation: Clin Cancer Res. 2009 Oct 1;15(19):6192-200
references.pmid: 19789312
references.citation: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D109-11
references.pmid: 14681370
references.citation: Exp Oncol. 2006 Dec;28(4):263-9
references.pmid: 17285108
references.citation: Exp Mol Pathol. 2010 Feb;88(1):45-51
references.pmid: 19874819
references.citation: J Dig Dis. 2010 Dec;11(6):323-33
references.pmid: 21091894
references.citation: Biol Cell. 2008 Feb;100(2):71-81
references.pmid: 18199046
references.citation: Am J Gastroenterol. 2005 Jun;100(6):1257-64
references.pmid: 15929754
references.citation: Curr Opin Pharmacol. 2009 Dec;9(6):721-6
references.pmid: 19828375
references.citation: Mayo Clin Proc. 1971 Nov;46(11):728-34
references.pmid: 5128394
references.citation: J Clin Pathol. 2009 Jan;62(1):84-8
references.pmid: 18755714
references.citation: JAMA. 2007 May 2;297(17):1901-8
references.pmid: 17473300
references.citation: Br J Surg. 1950 Oct;38(150):175-82
references.pmid: 14791960
references.citation: Hum Mol Genet. 2007 Apr 15;16 Spec No 1:R106-13
references.pmid: 17613543
references.citation: BMC Mol Biol. 2008 Jan 14;9:3
references.pmid: 18194512
references.citation: Eur J Surg Oncol. 2009 Apr;35(4):339-47
references.pmid: 18644693
references.citation: Acta Chir Scand. 1975;141(7):683-7
references.pmid: 1211042
references.citation: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D140-4
references.pmid: 16381832
references.citation: J Mol Diagn. 2008 May;10(3):203-11
references.pmid: 18403610