title: Novel mutations of PRSS1 gene in patients with pancreatic cancer among Han population.
abstract: BACKGROUND
  A high mortality rate of pancreatic cancer becomes a bottleneck for further treatment with long-term efficacy. It is urgent to find a new mean to predict the early onset of pancreatic cancer accurately. The authors hypothesized that genetic variants of cationic trypsinogen (PRSS1) gene could affect trypsin expression/function and result in abnormal activation of protease activated receptor-2 (PAR-2), then lead to pancreatic cancer. The aim of this study was to elaborate some novel mutations of PRSS1 gene in the patients with pancreatic cancer.
  
  
  METHODS
  Totally 156 patients with pancreatic cancer and 220 unrelated individuals as controls were enrolled in this study. The mutations of PRSS1 gene were analyzed by direct sequencing. K-ras Mutation Detection Kit was used to find the general k-ras gene disorder in the pancreatic cancer tissue. Then the clinical data were collected and analyzed simultaneously.
  
  
  RESULTS
  There were two patients who carried novel mutations which was IVS 3 + 157 G > C of PRSS1 gene in peripheral blood specimens and pancreatic cancer tissue. What's more, it was surprising to find a novel complicated mutation of exon 3 in PRSS1 gene (c.409 A > G and c.416 C > T) in another young patient. The complicated mutation made No. 135 and No. 137 amino acid transfer from Thr to Ala and Thr to Met respectively. No any mutation was found in the normal controls while no mutations of k-ras gene were detected in the three patients.
  
  
  CONCLUSIONS
  Mutations of PRSS1 gene may be an important factor of pancreatic cancer.
authors.affiliation: Department of Anesthesiology, the First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350005, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zeng
authors.forename: Qi-cai
authors.initials: QC
authors.lastname: Liu
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Lin
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Lin
authors.forename: Ze-hao
authors.initials: ZH
authors.lastname: Zhuang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Qi-shui
authors.initials: QS
authors.lastname: Ou
mesh_terms: D000328:Adult; D044466:Asian People; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014357:Trypsin / Q000235:genetics*
chemical_list: C489303:PRSS1 protein, human; D014357:Trypsin