title: Quantitation of amyloid beta peptides in CSF by surface enhanced MALDI-TOF.
abstract: Alzheimer's disease is characterized by the deposition of amyloid plaques in the brain. The major components of these plaques are β-amyloid (Aβ) peptides. The CSF concentration of these peptides can therefore provide a valuable biomarker for potentially predicting the state of disease and/or monitoring the efficacy of a drug aiming to inhibit the formation of amyloid plaques. Although the concentration of a given peptide in CSF can easily be measured by ELISA methods, few methods are able to simultaneously observe and distinguish between various peptides of similar yet slightly different amino acid composition. The Surface Enhanced Laser Desorption/Ionization-Time Of Flight mass spectrometry (SELDI-TOF) technology, a platform combining the use of an antibody and MALDI-TOF, can be used to simultaneously detect and quantitate various Aβ peptides with sensitivities in the picomolar range.
authors.affiliation: Wyeth Research, Collegeville, PA, USA. Takahashi.eddie@gmail.com
authors.forename: Eddie
authors.initials: E
authors.lastname: Takahashi
authors.forename: Anita
authors.initials: A
authors.lastname: Howe
authors.forename: Ole
authors.initials: O
authors.lastname: Vesterqvist
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D019963:Clinical Chemistry Tests / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D040081:Protein Array Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties
chemical_list: D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal