title: Differentiation of induced pluripotent stem cells into dental mesenchymal cells.
abstract: Similar to embryonic stem cells, induced pluripotent stem (iPS) cells can differentiate into various cell types upon appropriate induction, and thus, may be valuable cell sources for regenerative medicine. However, iPS cells have not been reported to differentiate into odontogenic cells for tooth regeneration. Here we demonstrated that neural crest-like cells (NCLC) derived from mouse iPS cells have the potential to differentiate into odontogenic mesenchymal cells. We developed an efficient culture protocol to induce the differentiation of mouse iPS cells into NCLC. We confirmed that the cells exhibited neural crest (NC) cell markers as evidenced by immunocytochemistry, flow cytometry, and real-time reverse transcription-polymerase chain reaction. Further, in recombination cultures of NCLC and mouse dental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.
authors.affiliation: Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University, Yahaba, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Otsu
authors.forename: Ryota
authors.initials: R
authors.lastname: Kishigami
authors.forename: Ai
authors.initials: A
authors.lastname: Oikawa-Sasaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Aya
authors.initials: A
authors.lastname: Yamada
authors.forename: Naoki
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Ishizeki
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Harada
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000378:metabolism; D015870:Gene Expression; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D009432:Neural Crest / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis; D013724:Teratoma / Q000473:pathology; D014083:Tooth Germ / Q000378:metabolism
chemical_list: D015415:Biomarkers