title: The selective cleanup of complex matrices and simultaneous separation of benzo[a]pyrene by solid-phase extraction with MgO microspheres as sorbents.
abstract: A new method for the selective cleanup of complex matrices and simultaneous separation of benzo[a]pyrene (BaP) was developed in this study. This method was based on solid-phase extraction (SPE) using magnesium oxide microspheres as sorbents, and it eliminated interferences from various impurities, such as lipids, sulphur, pigments, halobenzenes, polychlorodibenzo-p-dioxins and polychlorodibenzofurans. Several parameters, including the volume of rinsing and eluting solvents, the type of loading solvents and SPE sorbents, were optimized systematically. The capability for impurity removal was verified by gel permeation chromatography, gas chromatography, and liquid chromatography. Compared to commercial sorbents (silica gel, florisil and alumina), MgO microspheres exhibited excellent performance in the selective isolation of BaP and removal of impurities. The proposed method was applied to detect BaP in complex samples (sediments, soils, fish, and porcine liver). The limit of quantification (LOQ) was 1.04 ngL(-1), and the resulting regression coefficient (r(2)) was greater than 0.999 over a broad concentration range (9.5-7600 ngL(-1)). In contrast to traditional methods, the proposed method can give rise to higher recovery (85.1-100.8%) and better selectivity with simpler operation and less consumption of organic solvents (20-40 mL).
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Su
authors.forename: Peipei
authors.initials: P
authors.lastname: Qi
authors.forename: Xianbo
authors.initials: X
authors.lastname: Lu
authors.forename: Jiping
authors.initials: J
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005399:Fishes; D019015:Geologic Sediments / Q000737:chemistry; D008055:Lipids / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry; D008277:Magnesium Oxide / Q000737:chemistry*; D008863:Microspheres; D010860:Pigments, Biological / Q000302:isolation & purification; D012987:Soil / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D013455:Sulfur / Q000302:isolation & purification; D013552:Swine
chemical_list: D008055:Lipids; D010860:Pigments, Biological; D012987:Soil; D001564:Benzo(a)pyrene; D008277:Magnesium Oxide; D013455:Sulfur