title: Enzymatic routes for the production of mono- and di-glucosylated derivatives of hydroxytyrosol.
abstract: In this work, a new eco-friendly procedure for the synthesis of hydroxytyrosol and tyrosol α-glycosidic derivatives was proposed by using the marine α-glucosidase from Aplysia fasciata, and a commercial tyrosinase from mushroom for the bioconversion of tyrosol glycosidic derivatives into the corresponding hydroxytyrosol products. New hydroxytyrosol mono- and di-saccharide derivatives were synthesized at final concentrations of 9.35 and 10.8 g/l of reaction, respectively, and their antioxidant activity was evaluated by DPPH test. The best antioxidant agent resulted the (3,4-dihydroxyphenyl) ethyl-α-D-glucopyranoside; it showed a radical scavenging activity similar to that of the hydroxytyrosol, together with an increased hydrosolubility. This molecule could be a good response to many food industry demands, always in search of cheap antioxidants with nutritional properties to improve the nutritional value and the quality of foods.
authors.affiliation: Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Trincone
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pagnotta
authors.forename: Annabella
authors.initials: A
authors.lastname: Tramice
mesh_terms: D000818:Animals; D001048:Aplysia / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006027:Glycosides / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D016166:Free Radical Scavengers; D006027:Glycosides; C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; D000520:alpha-Glucosidases; D010626:Phenylethyl Alcohol