title: Determination of acrylamide in starch-based foods by HPLC with pre-column ultraviolet derivatization.
abstract: A new method is developed for the determination of acrylamide in starch-based foods. The method included the extraction of acrylamide with water, defatting with hexane, derivatization with potassium bromate (KBrO(3)) and potassium bromide (KBr), liquid-liquid extraction with ethyl acetate-hexane (4:1), and concentration. The final analyte (2-bromopropenamide, 2-BPA) is analyzed by high-performance liquid chromatography coupled with diode array detection for quantification and by gas chromatography coupled to mass spectrometry for confirmation. The chromatographic analysis is performed on an ODS-3 C(18) column, and good retention and peak response of acrylamide are achieved under the optimal conditions. The limit of detection and quantitation are estimated to be 15 and 50 μg/kg, respectively. The recoveries of acrylamide from the commercial samples are spiked at levels of 50-1000 μg/kg, and range between 89.6 and 102.0%. These results show that this method should be regarded as a new, low-cost, and robust alternative for conventional investigation of acrylamide.
authors.affiliation: Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, China. zmgeng@163.com
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Geng
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Aiming
authors.initials: A
authors.lastname: Liu
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D054879:Halogenation; D059625:Liquid-Liquid Extraction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013213:Starch / Q000737:chemistry*
chemical_list: D020106:Acrylamide; D013213:Starch