title: Parasitic helminth cystatin inhibits DSS-induced intestinal inflammation via IL-10(+)F4/80(+) macrophage recruitment.
abstract: Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-γ and TNF-α in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-α in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10(+)F4/80(+) macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages.
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-813, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Jang
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Cho
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kang
authors.forename: Byoung-Kuk
authors.initials: BK
authors.lastname: Na
authors.forename: Soon Cheol
authors.initials: SC
authors.lastname: Ahn
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D003004:Clonorchis sinensis / Q000201:enzymology*; D003106:Colon / Q000473:pathology; D015891:Cystatins / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D015801:Helminth Proteins / Q000378:metabolism*; D007155:Immunologic Factors / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D016753:Interleukin-10 / Q000032:analysis; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology; D008264:Macrophages / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012720:Severity of Illness Index; D013154:Spleen / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D015891:Cystatins; D016207:Cytokines; D015801:Helminth Proteins; C556062:IL10 protein, mouse; D007155:Immunologic Factors; C036763:monocyte-macrophage differentiation antigen; D016753:Interleukin-10; D016264:Dextran Sulfate
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