title: Salt induces biosynthesis of hemolytically active compounds in the xerotolerant food-borne fungus Wallemia sebi.
abstract: Wallemia sebi is a xerotolerant, ubiquitous, food-borne, mycotoxigenic fungus. An ethanol extract of its mycelium demonstrated a strong hemolytic activity, which was further enhanced at high salt concentrations in the growth medium. Characterization of the extract using gas chromatography-mass spectrometry revealed a mixture of sterols and unsaturated fatty acids, indicating the latter as responsible for the hemolytic activity. The lytic activity of the extract is here studied using red blood cells and artificial small lipid vesicles with various lipid compositions. This shows concentration-dependent hemolysis and preferential activity toward lipid membranes with greater fluidity. The W. sebi lytic activity on mammalian erythrocytes shows its potential involvement in the formation of lesions in subcutaneous infections, in farmer's lung disease, and in consumption of food and feed that are contaminated with food-borne W. sebi.
authors.affiliation: Laboratory for Separation Processes and Product Design, Faculty for Chemistry and Chemical Engineering, University of Maribor, Maribor, Slovenia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Botić
authors.forename: Marjetka K
authors.initials: MK
authors.lastname: Kunčič
authors.forename: Kristina
authors.initials: K
authors.lastname: Sepčić
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Knez
authors.forename: Nina
authors.initials: N
authors.lastname: Gunde-Cimerman
mesh_terms: D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development*; D004912:Erythrocytes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005516:Food Microbiology; D008401:Gas Chromatography-Mass Spectrometry; D006461:Hemolysis; D008560:Membrane Fluidity; D008567:Membranes, Artificial; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D008567:Membranes, Artificial; D012965:Sodium Chloride