title: Irreversible electroporation shows efficacy against pancreatic carcinoma without systemic toxicity in mouse models.
abstract: Pancreatic ductal adenocarcinoma (PDAC) therapies show limited success. Irreversible electroporation (IRE) is an innovative loco-regional therapy in which high-voltage pulses are applied to induce plasma membrane defects leading to cellular death. In the present study we evaluated the feasibility of IRE against PDAC. IRE treatment exhibited significant antitumor effects and prolonged survival in mice with orthotopic xenografts. Extensive tumor necrosis, reduced tumor cell proliferation and disruption of microvessels were observed at different days post-IRE. Animals had transient increases in transaminases, amylase and lipase enzymes that normalized at 24h post-IRE. These results suggest that IRE could be an effective treatment for locally advanced pancreatic tumors.
authors.affiliation: Institut d'Investigacions Biomèdiques August Pi i Sunyer-IDIBAPS, Barcelona, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: José
authors.forename: Luciano
authors.initials: L
authors.lastname: Sobrevals
authors.forename: Antoni
authors.initials: A
authors.lastname: Ivorra
authors.forename: Cristina
authors.initials: C
authors.lastname: Fillat
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000681:Amylases / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000098:blood supply / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053672:Electrochemotherapy / Q000009:adverse effects; D005240:Feasibility Studies; D017930:Genes, Reporter; D006801:Humans; D008049:Lipase / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008819:Mice, Nude; D055806:Microvessels / Q000473:pathology; D009336:Necrosis; D010190:Pancreatic Neoplasms / Q000097:blood / Q000098:blood supply / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D013997:Time Factors; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008049:Lipase; D000681:Amylases