title: Suppression of epidermal growth factor receptor (EGFR) expression by small hairpin RNA inhibits the growth of human nonsmall cell lung cancers bearing wild-type and mutant EGFR.
abstract: In the present study, we have used plasmid-based RNA interference (RNAi) strategy to downregulate the expression of epidermal growth factor receptor (EGFR) in EGFR wild-type (H292) and mutant (H1975) lung tumor models. The targeted knockdown of EGFR by small hairpin RNA not only inhibited growth of H292 xenograft but also inhibited H1975 lung cancer cell and xenograft, which bore L858R/T790M EGFR and was resistant to EGFR tyrosine kinase inhibitors. These data demonstrated that small hairpin RNA was an effective therapy against mutant EGFR-expressing cancer cells and thus considered to be a promising strategy in the treatment of lung cancers.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tian
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.forename: Lei
authors.initials: L
authors.lastname: Dai
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
authors.forename: Guobo
authors.initials: G
authors.lastname: Shen
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Qiaorong
authors.initials: Q
authors.lastname: Huang
authors.forename: Yalin
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongping
authors.initials: Y
authors.lastname: Ma
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Shengyong
authors.initials: S
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lixia
authors.initials: L
authors.lastname: Dai
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Hongxin
authors.initials: H
authors.lastname: Deng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000235:genetics*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage; D005260:Female; D006801:Humans; D008081:Liposomes; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051379:Mice; D009154:Mutation; D009361:Neoplasm Invasiveness; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; D066246:ErbB Receptors; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane