title: Expressed sequence tag analysis of the erythrocytic stage of Plasmodium berghei.
abstract: Rodent malaria parasites, such as Plasmodium berghei, are practical and useful model organisms for human malaria research because of their analogies to the human malaria in terms of structure, physiology, and life cycle. Exploiting the available genetic sequence information, we constructed a cDNA library from the erythrocytic stages of P. berghei and analyzed the expressed sequence tag (EST). A total of 10,040 ESTs were generated and assembled into 2,462 clusters. These EST clusters were compared against public protein databases and 48 putative new transcripts, most of which were hypothetical proteins with unknown function, were identified. Genes encoding ribosomal or membrane proteins and purine nucleotide phosphorylases were highly abundant clusters in P. berghei. Protein domain analyses and the Gene Ontology functional categorization revealed translation/protein folding, metabolism, protein degradation, and multiple family of variant antigens to be mainly prevalent. The presently-collected ESTs and its bioinformatic analysis will be useful resources to identify for drug target and vaccine candidates and validate gene predictions of P. berghei.
authors.affiliation: Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Seok
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Moon
authors.forename: Jung-Yub
authors.initials: JY
authors.lastname: Lee
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Jha
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Kong
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Chung
authors.forename: Yeonchul
authors.initials: Y
authors.lastname: Hong
mesh_terms: D000818:Animals; D019295:Computational Biology; D004912:Erythrocytes / Q000469:parasitology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D010962:Plasmodium berghei / Q000235:genetics* / Q000472:pathogenicity*
references.citation: J Biol Chem. 2002 Feb 1;277(5):3226-31
references.pmid: 11706018
references.citation: J Biol Chem. 2010 Dec 3;285(49):37964-75
references.pmid: 20837488
references.citation: Nature. 2002 Oct 3;419(6906):498-511
references.pmid: 12368864
references.citation: Adv Parasitol. 2003;54:255-80
references.pmid: 14711087
references.citation: Philos Trans R Soc Lond B Biol Sci. 2010 Mar 12;365(1541):749-63
references.pmid: 20124342
references.citation: Gene. 2002 May 29;291(1-2):105-13
references.pmid: 12095684
references.citation: Mol Biochem Parasitol. 2005 Feb;139(2):185-95
references.pmid: 15664653
references.citation: Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W5-9
references.pmid: 18440982
references.citation: Mol Biochem Parasitol. 2005 Nov;144(1):1-9
references.pmid: 16085323
references.citation: Biochem Pharmacol. 1994 Aug 3;48(3):495-503
references.pmid: 7520696
references.citation: Proc Biol Sci. 2002 Feb 22;269(1489):431-6
references.pmid: 11886633
references.citation: Nucleic Acids Res. 2010 Jan;38(Database issue):D211-22
references.pmid: 19920124
references.citation: Traffic. 2008 Feb;9(2):166-75
references.pmid: 17900270
references.citation: Toxicol Lett. 2004 Apr 1;149(1-3):123-31
references.pmid: 15093257
references.citation: J Mol Biol. 1996 Aug 16;261(2):155-72
references.pmid: 8757284
references.citation: J Biomed Sci. 2002 Jan-Feb;9(1):34-40
references.pmid: 11810023
references.citation: Genome Biol. 2000;1(4):REVIEWS1026
references.pmid: 11178253
references.citation: Mol Biochem Parasitol. 1994 Dec;68(2):285-96
references.pmid: 7739674
references.citation: Bioinformatics. 2003 Mar 22;19(5):651-2
references.pmid: 12651724
references.citation: J Biol Chem. 2002 Feb 1;277(5):3219-25
references.pmid: 11707439
references.citation: Science. 2005 Jan 7;307(5706):82-6
references.pmid: 15637271
references.citation: Biochim Biophys Acta. 2001 Dec 12;1541(1-2):34-53
references.pmid: 11750661
references.citation: Genome Res. 2003 Mar;13(3):443-54
references.pmid: 12618375
references.citation: Methods Mol Biol. 2007;396:59-70
references.pmid: 18025686
references.citation: Genome Res. 1998 Mar;8(3):186-94
references.pmid: 9521922
references.citation: Mol Biochem Parasitol. 1994 Jul;66(1):97-104
references.pmid: 7984191
references.citation: Exp Parasitol. 1960 Apr;9:131-6
references.pmid: 13803493
references.citation: J Biol Chem. 2010 Oct 22;285(43):33054-33064
references.pmid: 20702404
references.citation: Nat Rev Microbiol. 2004 Mar;2(3):203-16
references.pmid: 15083156
references.citation: Bioinformatics. 2005 Sep 15;21(18):3674-6
references.pmid: 16081474
references.citation: Eukaryot Cell. 2008 Aug;7(8):1231-7
references.pmid: 18567789
references.citation: Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14548-53
references.pmid: 16983082
references.citation: Infect Disord Drug Targets. 2010 Jun;10(3):217-25
references.pmid: 20334618
references.citation: Genome Res. 1998 Mar;8(3):175-85
references.pmid: 9521921
references.citation: Mol Biochem Parasitol. 2001 Dec;118(2):201-10
references.pmid: 11738710