title: PAN hollow fiber membranes elicit functional hippocampal neuronal network.
abstract: This study focuses on the development of an advanced in vitro biohybrid culture model system based on the use of hollow fibre membranes (HFMs) and hippocampal neurons in order to promote the formation of a high density neuronal network. Polyacrylonitrile (PAN) and modified polyetheretherketone (PEEK-WC) membranes were prepared in hollow fibre configuration. The morphological and metabolic behaviour of hippocampal neurons cultured on PAN HF membranes were compared with those cultured on PEEK-WC HF. The differences of cell behaviour between HFMs were evidenced by the morphometric analysis in terms of axon length and also by the investigation of metabolic activity in terms of neurotrophin secretion. These findings suggested that PAN HFMs induced the in vitro reconstruction of very highly functional and complex neuronal networks. Thus, these biomaterials could potentially be used for the in vitro realization of a functional hippocampal tissue analogue for the study of neurobiological functions and/or neurodegenerative diseases.
authors.affiliation: Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Rende, CS, Italy. s.morelli@itm.cnr.it
authors.forename: Sabrina
authors.initials: S
authors.lastname: Morelli
authors.forename: Antonella
authors.initials: A
authors.lastname: Piscioneri
authors.forename: Simona
authors.initials: S
authors.lastname: Salerno
authors.forename: Franco
authors.initials: F
authors.lastname: Tasselli
authors.forename: Anna
authors.initials: A
authors.lastname: Di Vito
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Giusi
authors.forename: Marcello
authors.initials: M
authors.lastname: Canonaco
authors.forename: Enrico
authors.initials: E
authors.lastname: Drioli
authors.forename: Loredana
authors.initials: L
authors.lastname: De Bartolo
mesh_terms: D000818:Animals; D006224:Cricetinae; D006624:Hippocampus / Q000166:cytology*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009415:Nerve Net
chemical_list: D008567:Membranes, Artificial
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