title: Detection of IL-1β in culture media supernatants of pre-implantation human embryos; its relation with embryo grades and development.
abstract: In this study, we aimed to determine whether human embryos secrete interleukin-1β (IL-1β) into culture media and its correlation with embryo grade and development. Culture media supernatants of 100 embryos obtained from 39 cycles of 38 patients and cultivated individually were collected 2 and 3 days after intracytoplasmic sperm injection (ICSI). IL-1β concentrations of samples were determined with ELISA and compared with embryo grades and blastomere numbers. Embryo grades and the amount of IL-1β they secreted were found not to be correlated (p:0.559). Numbers of blastomeres each embryo had at 2nd and 3rd days were found to be correlated with IL-1β secreted (p:0.00 and p:0.00, respectively). Mean amount of IL-1β secreted by the embryos from ejaculated sperm cycles were found to be significantly higher than that of embryos from TESE cycles (p:0.016). Patient age and etiology of infertility were not correlated with the amount of IL-1β secreted and embryo grade. In conclusion, preimplantation human embryos secrete IL-1β in their media in amounts correlated with their blastomere numbers.
authors.affiliation: Department of Obstetrics and Gynecology, Infertility Research Center, Medical School of Ankara University, Ankara, Turkey. eclamptic2000@yahoo.com
authors.forename: Elif Aylin
authors.initials: EA
authors.lastname: Taşkın
authors.forename: Volkan
authors.initials: V
authors.lastname: Baltacı
authors.forename: Gamze
authors.initials: G
authors.lastname: Cağıran
authors.forename: Ruşen
authors.initials: R
authors.lastname: Aytaç
mesh_terms: D001755:Blastocyst / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D047108:Embryonic Development / Q000502:physiology*; D005307:Fertilization in Vitro; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis* / Q000378:metabolism; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D003470:Culture Media; D053583:Interleukin-1beta