title: The pleiohomeotic functions as a negative regulator of Drosophila even-skipped gene during embryogenesis.
abstract: Polycomb group (PcG) proteins maintain the spatial expression patterns of genes that are involved in cell-fate specification along the anterior-posterior (A/P) axis. This repression requires cis-acting silencers, which are called PcG response elements (PREs). One of the PcG proteins, Pleiohomeotic (Pho), which has a zinc finger DNA binding protein, plays a critical role in recruiting other PcG proteins to bind to PREs. In this study, we characterized the effects of a pho mutation on embryonic segmentation. pho maternal mutant embryos showed various segmental defects including pair-rule gene mutant patterns. Our results indicated that engrailed and even-skipped genes were misexpressed in pho mutant embryos, which caused embryonic segment defects.
authors.affiliation: Department of Biology Education, Seoul National University, Seoul 151-748, Korea.
authors.forename: Su Na
authors.initials: SN
authors.lastname: Kim
authors.forename: Hyun Pyo
authors.initials: HP
authors.lastname: Shim
authors.forename: Bu-Nam
authors.initials: BN
authors.lastname: Jeon
authors.forename: Won-Il
authors.initials: WI
authors.lastname: Choi
authors.forename: Man-Wook
authors.initials: MW
authors.lastname: Hur
authors.forename: Jack R
authors.initials: JR
authors.lastname: Girton
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Sang-Hak
authors.initials: SH
authors.lastname: Jeon
mesh_terms: D000818:Animals; D001756:Blastoderm / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000033:anatomy & histology / Q000378:metabolism; D009154:Mutation; D063146:Polycomb-Group Proteins; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C063459:En protein, Drosophila; D018398:Homeodomain Proteins; D063146:Polycomb-Group Proteins; D014157:Transcription Factors; C052837:eve protein, Drosophila; C113295:pho protein, Drosophila
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