title: Glutamine requirement for aerial mycelium growth in Neurospora crassa.
abstract: Five amino acids are accumulated during vegetative growth of Neurospora crassa, particularly.during the prestationary growth phase. Alanine, glutamine, glutamate, arginine and ornithine.comprised over 80% of the total amino acid pool in the mycelium. Amino acid pools of different amino acid auxotrophs were followed during the partial transformation of a mycelial mat into an aerial mycelium. The mycelial mat under starvation and in direct contact with air rapidly formed aerial mycelium, which produced thereafter a burst of conidia. During this process,glutamine and alanine in the mycelial mat were consumed more rapidly than other amino acids;in the growing aerial mycelium, glutamate and glutamine were particularly accumulated. Of the amino acids that were initially accumulated in the mycelial mat, only a high glutamine pool was required for aerial mycelium growth induced by starvation. This requirement for glutamine could not be satisfied by a mixture of the amino compounds that are synthesized via glutamine amidotransferase reactions. It is proposed that glutamine serves as a nitrogen carrier from the mycelial mat to the growing aerial mycelium.
authors.affiliation: Centro de Investigacibn sobre Fijacibn de Nitrbgeno, Universidad Nacional Autbnoma de Mkxico,Apartado Postal .56.5-A-Cuernavac~M, or., Mexico.
authors.forename: M E
authors.initials: ME
authors.lastname: Cárdenas
authors.forename: W
authors.initials: W
authors.lastname: Hansberg
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018086:Glutamate Dehydrogenase (NADP+) / Q000235:genetics / Q000378:metabolism; D005970:Glutamate Synthase / Q000235:genetics / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005656:Fungal Proteins; D005973:Glutamine; D018698:Glutamic Acid; D005970:Glutamate Synthase; D018086:Glutamate Dehydrogenase (NADP+); D005974:Glutamate-Ammonia Ligase; D000409:Alanine