title: Peripheral CD300a+CD8+ T lymphocytes with a distinct cytotoxic molecular signature increase in pregnant women with chronic chorioamnionitis.
abstract: OBJECTIVE
  CD300a is an immunomodulatory molecule of the immunoglobulin receptor superfamily expressed in the leukocytes of myeloid and lymphoid lineages. However, its biological function on CD8+ T lymphocytes remains largely unknown. This study was conducted to assess the biological significance of CD300a expression in T lymphocytes and to determine whether its expression in peripheral T lymphocytes changes in pregnant women presenting with antifetal rejection.
  
  
  METHODS
  Microarray analysis was performed using total RNA isolated from peripheral CD300a+ and CD300a- T lymphocytes. Flow cytometric analysis of the peripheral blood samples of pregnant women and pathologic examination of the placentas were conducted.
  
  
  RESULTS
  A large number of genes (N = 1245) were differentially expressed between CD300a- and CD300a+ subsets of CD8+ T lymphocytes, which included CCR7, CD244, CX3CR1, GLNY, GZMB, GZMK, IL15, ITGB1, KLRG1, PRF1, and SLAMF7. Gene ontology analysis of differentially expressed genes demonstrated enrichment of biological processes such as immune response, cell death, and signal transduction. CD300a expression in CD8+ T lymphocytes was coupled to a more cytotoxic molecular signature. Of note, the proportion of CD300a+CD8+ T lymphocytes increased in pregnant women with chronic chorioamnionitis (antifetal rejection of the chorioamniotic membranes; P < 0.05).
  
  
  CONCLUSIONS
  The findings of this study strongly suggest an increase in systemic T-lymphocyte-mediated cytotoxicity in pregnant women with chronic chorioamnionitis as a manifestation of maternal antifetal rejection.
authors.affiliation: Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Federica
authors.initials: F
authors.lastname: Tarquini
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
authors.forename: Adi L
authors.initials: AL
authors.lastname: Tarca
authors.forename: Gaurav
authors.initials: G
authors.lastname: Bhatti
authors.forename: JoonHo
authors.initials: J
authors.lastname: Lee
authors.forename: I Birgitta
authors.initials: IB
authors.lastname: Sundell
authors.forename: Pooja
authors.initials: P
authors.lastname: Mittal
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Kusanovic
authors.forename: Sonia S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002821:Chorioamnionitis / Q000097:blood / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C515070:CD300A protein, human; D011971:Receptors, Immunologic
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