title: Research on basis of reverse genetics system of a Sindbis-like virus XJ-160.
abstract: As a Sindbis-like virus (SINLV), XJ-160 virus was isolated from a pooled sample of Anopheles mosquitoes collected in Xinjiang, China, in 1990. Recombinant plasmid pBR-XJ160 is an infectious full-length cDNA clone of XJ-160 virus, from which rescued virus BR-XJ160 can be obtained by transcription in vitro and transfection. The BR-XJ160 virus raised in BHK-21 cells was indistinguishable from the XJ-160 virus in its biological properties, including its plaque morphology, growth kinetics and suckling mouse neurovirulence. On basis of pBR-XJ160, the effects of substitutions within nonstructural protein 1 (nsP1) or nsP2 on the infectivity and pathogenesis of Sindbis virus (SINV) have been investigated. We have also confirmed the essential role of E2 glycoprotein, especially the domain of 145-150 (amino acid) aa, in SINV infection through the interaction with cellular heparan sulfate (HS). In addition, we have developed XJ-160 virus-based vector system, including replicon vector, defective helper (DH) plasmids and the packaging cell lines (PCLs). Here we provide an update of main development in the field concerned with XJ-160 virus.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Viral Disease Control and Prevention, Beijing 100052, China. gdliang@hotmail.com
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wu-yang
authors.forename: Liang
authors.initials: L
authors.lastname: Guo-dong
mesh_terms: D000818:Animals; D000852:Anopheles / Q000821:virology; D002681:China; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010957:Plasmids; D059386:Reverse Genetics / Q000379:methods*; D012845:Sindbis Virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism; D010948:Viral Plaque Assay; D014773:Virology / Q000379:methods*; D014774:Virulence; D019065:Virus Assembly
chemical_list: D018076:DNA, Complementary; D050505:Mutant Proteins; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C064950:glycoprotein E2, Sindbis virus; C104211:nsP1 protein, Sindbis virus; D003546:Cysteine Endopeptidases; C078306:nsP2 proteinase
references.citation: J Gen Virol. 2000 May;81(Pt 5):1347-51
references.pmid: 10769078
references.citation: Emerg Infect Dis. 2008 Mar;14(3):454-60
references.pmid: 18325261
references.citation: J Virol. 2002 Oct;76(20):10128-37
references.pmid: 12239287
references.citation: PLoS One. 2010 Mar 11;5(3):e9656
references.pmid: 20300181
references.citation: Virol J. 2011 Jul 10;8:344
references.pmid: 21740598
references.citation: Annu Rev Entomol. 2004;49:141-74
references.pmid: 14651460
references.citation: Virology. 1990 Oct;178(2):429-34
references.pmid: 2219702
references.citation: J Virol. 2001 Jul;75(14):6303-9
references.pmid: 11413296
references.citation: Virology. 1989 Jun;170(2):385-91
references.pmid: 2728344
references.citation: Mol Ther. 2003 Feb;7(2):202-9
references.pmid: 12597908
references.citation: Arch Virol. 2009;154(2):245-53
references.pmid: 19118404
references.citation: Virol J. 2010 Sep 14;7:225
references.pmid: 20836893
references.citation: Emerg Infect Dis. 2009 Apr;15(4):519-25
references.pmid: 19331726
references.citation: Virology. 1996 Mar 15;217(2):527-31
references.pmid: 8610444
references.citation: Virology. 1991 Sep;184(1):423-7
references.pmid: 1831311
references.citation: Bing Du Xue Bao. 2010 May;26(3):228-33
references.pmid: 20572345
references.citation: Science. 1989 Mar 3;243(4895):1188-91
references.pmid: 2922607
references.citation: Virology. 1997 Feb 3;228(1):74-83
references.pmid: 9024811
references.citation: Microbiol Rev. 1994 Sep;58(3):491-562
references.pmid: 7968923
references.citation: J Virol. 2000 Oct;74(20):9802-7
references.pmid: 11000258
references.citation: Intervirology. 2009;52(2):100-6
references.pmid: 19420962
references.citation: Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):12989-94
references.pmid: 9789028
references.citation: J Virol. 1998 Sep;72(9):7349-56
references.pmid: 9696831
references.citation: Bing Du Xue Bao. 2009 Mar;25(2):143-7
references.pmid: 19678570
references.citation: Virus Res. 2009 Jun;142(1-2):204-7
references.pmid: 19428754
references.citation: J Virol. 1991 Feb;65(2):985-8
references.pmid: 1824787
references.citation: Virology. 1994 Feb;198(2):415-26
references.pmid: 8291226
references.citation: J Virol. 1998 Sep;72(9):7357-66
references.pmid: 9696832
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11371-7
references.pmid: 8876142