title: Expression of the prion-like protein Shadoo in the developing mouse embryo.
abstract: The prion-like protein Shadoo has been suggested to compensate for the lack of PrP in Prnp-knockout mice, explaining their lack of extreme phenotype. In adult mice, both PrP and Shadoo have shown overlapping expression patterns and shared functions. Their expression in the mouse embryo has also been suggested to be complementary, as invalidation of both genes results in embryonic lethality. The developmental expression profile of PrP has been described from post-implantation stages up until birth. However the spatial expression pattern of Shadoo in the developing mouse embryo is not known. We previously described the expression profile of the prion-like protein Shadoo in adult mice using Sprn reporter mice (Sprn-GFP and Sprn-LacZ). Here we used these mice to describe the developmental expression of Shadoo between 10.5 and 14.5 dpc. The observed pattern in specific embryonic cell lineages and in extra-embryonic tissues is consistent with the previously reported phenotype resulting from its knockdown.
authors.affiliation: INRA UMR 1313, Equipe Différenciation et Spécialisation Cellulaires (DISC), Génétique Animale et Biologie Intégrative (GABI), 78350 Jouy-en-Josas, France. rachel.young@jouy.inra.fr
authors.forename: Rachel
authors.initials: R
authors.lastname: Young
authors.forename: Stéphan
authors.initials: S
authors.lastname: Bouet
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Polyte
authors.forename: Sandrine
authors.initials: S
authors.lastname: Le Guillou
authors.forename: Bruno
authors.initials: B
authors.lastname: Passet
authors.forename: Marthe
authors.initials: M
authors.lastname: Vilotte
authors.forename: Johan
authors.initials: J
authors.lastname: Castille
authors.forename: Vincent
authors.initials: V
authors.lastname: Beringue
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Provost
authors.forename: Hubert
authors.initials: H
authors.lastname: Laude
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vilotte
mesh_terms: D000818:Animals; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D058851:GPI-Linked Proteins; D055785:Gene Knockdown Techniques; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000096:biosynthesis / Q000235:genetics; D019076:Transgenes; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; C523856:Sprn protein, mouse; D001616:beta-Galactosidase