title: Controlled gene expression in bifidobacteria by use of a bile-responsive element.
abstract: The promoter activity of the upstream region of the bile-inducible gene betA from Bifidobacterium longum subsp. longum NCC2705 was characterized. DNA fragments were cloned into the reporter vector pMDYAbfB, and the arabinofuranosidase activity was determined under different in vitro conditions. A segment of 469 bp was found to be the smallest operational unit that retains bile inducibility. The reporter activity was strongly affected by the presence of ox gall, cholate, and conjugated cholate, but not by other bile salts and cell-surface-acting compounds. Remarkably, this bile-inducible system was also active in other bifidobacteria containing betA homologs.
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Ruiz
authors.forename: Pablo
authors.initials: P
authors.lastname: Álvarez-Martín
authors.forename: Baltasar
authors.initials: B
authors.lastname: Mayo
authors.forename: Clara G
authors.initials: CG
authors.lastname: de los Reyes-Gavilán
authors.forename: Miguel
authors.initials: M
authors.lastname: Gueimonde
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
mesh_terms: D001644:Bifidobacterium / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D001646:Bile / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression / Q000187:drug effects*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
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