title: The effects of bare metal versus drug-eluting stent implantation on circulating endothelial cells following percutaneous coronary intervention.
abstract: BACKGROUND
  The purpose of this study was to compare the effects of bare metal stents (BMS) and drug-eluting stents (DES) implantation on circulating endothelial cells (CECs) which have been proposed as cellular markers of endothelial dysfunction following percutaneous coronary intervention (PCI). Recently, it has been established that DES further reduce restenosis and revascularization rate compared to bare metal stents in elective procedures. However, its benefits are compromised by the stent-related thrombosis events.
  
  
  METHODS
  22 patients who were candidate of PCI were included in this study. The patients underwent DES implantation (n = 11) or BMS implantation (n = 11). In all patients the numbers of CECs were determined before and a week after stent implantation using flow cytometry and the obtained data were compared within and between groups by paired and unpaired Student's t-test, respectively. CECs were defined as cells negative for CD45 (FITC) and highly double positive for CD146 (PE) and CD34 (PE-Cy5) expression.
  
  
  RESULTS
  There were no significant differences in the baseline levels of CECs between two groups (p = 0.96). Stent implantation led to a significant increase in CECs compared with the preprocedural levels in the BMS group (p = 0.005) whereas there was a significant decrease in CEC numbers in DES group (p < 0.001). One week after stent implantation CECs count in BMS group was significantly higher compared to DES group (p < 0.001).
  
  
  CONCLUSIONS
  The results indicate that patients undergoing DES implantation were subjected to less endothelial injury than patients receiving BMS as indicated by CEC enumeration.
authors.affiliation: Applied Physiology Research Center, Department of Physiology and Department of Cardiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Seyed Mohammad Hashemi
authors.initials: SM
authors.lastname: Jazi
authors.forename: Saeed
authors.initials: S
authors.lastname: Shafiei
authors.forename: Seyed Hamid
authors.initials: SH
authors.lastname: Zarkesh-Esfahani
authors.forename: Saman
authors.initials: S
authors.lastname: Maleki Vareki
authors.forename: Shaghayegh Haghjooy
authors.initials: SH
authors.lastname: Javanmard
references.citation: J Cardiovasc Pharmacol. 2006 Dec;48(6):286-92
references.pmid: 17204907
references.citation: Am J Cardiol. 2011 Jan 15;107(2):210-4
references.pmid: 21129713
references.citation: Annu Rev Physiol. 2011;73:503-14
references.pmid: 20887194
references.citation: Am J Cardiol. 2006 May 1;97(9):1311-6
references.pmid: 16635602
references.citation: J Am Coll Cardiol. 2006 Oct 17;48(8):1538-47
references.pmid: 17045885
references.citation: Circulation. 2007 Feb 27;115(8):1051-8
references.pmid: 17325255
references.citation: Nephrol Dial Transplant. 2002 Oct;17(10):1728-30
references.pmid: 12270976
references.citation: Int Heart J. 2007 Sep;48(5):553-67
references.pmid: 17998765
references.citation: J Am Coll Cardiol. 2007 Oct 2;50(14):1305-9
references.pmid: 17903627
references.citation: J Am Coll Cardiol. 2005 Jul 19;46(2):231-6
references.pmid: 16022947
references.citation: Chest. 2007 Dec;132(6):1920-6
references.pmid: 18079225
references.citation: Clin Chim Acta. 2006 Nov;373(1-2):17-26
references.pmid: 16836991
references.citation: Eur Heart J. 2005 Apr;26(8):804-47
references.pmid: 15769784
references.citation: Eur Heart J. 2006 Jan;27(2):166-70
references.pmid: 16249221
references.citation: Thromb Haemost. 2006 Jul;96(1):45-52
references.pmid: 16807650
references.citation: Atherosclerosis. 2008 Jun;198(2):366-72
references.pmid: 17959181
references.citation: Nat Med. 2002 Feb;8(2):128-35
references.pmid: 11821896
references.citation: Am J Cardiol. 2005 Nov 15;96(10):1384-8
references.pmid: 16275183
references.citation: Circulation. 2006 Feb 21;113(7):e166-286
references.pmid: 16490830
references.citation: Clin Sci (Lond). 2008 Feb;114(4):265-73
references.pmid: 18194135
references.citation: FASEB J. 2005 Feb;19(2):246-8
references.pmid: 15546959
references.citation: J Am Coll Cardiol. 1998 Oct;32(4):1109-17
references.pmid: 9768740
references.citation: Eur J Immunol. 1994 Dec;24(12):3211-5
references.pmid: 7805749