title: Nuclear and chloroplast SSR markers in Paeonia delavayi (Paeoniaceae) and cross-species amplification in P. ludlowii.
abstract: OBJECTIVE
  Microsatellite primers were developed for Paeonia delavayi and P. ludlowii (Paeoniaceae) to study their population genetics and phytogeography.
  
  
  RESULTS
  Nine polymorphic nuclear microsatellite loci were isolated from an enriched library of P. delavayi and primers were designed. The number of alleles per locus ranged from two to 16; the observed and expected heterozygosities ranged from 0.014 to 0.687 and 0.042 to 0.875, respectively. Six polymorphic chloroplast microsatellite loci were identified in P. delavayi and primers were provided. The number of alleles per locus ranged from two to six and the polymorphic information content ranged from 0.08 to 0.716. Both nuclear and chloroplast primers were successfully applicable to P. ludlowii.
  
  
  CONCLUSIONS
  The markers developed here will facilitate analyses of genetic diversity, population genetic structure, phytogeographical patterns, and conservation for P. delavayi and P. ludlowii.
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, China.
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hai-Lu
authors.initials: HL
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Jian-Xiu
authors.initials: JX
authors.lastname: Wang
authors.forename: Shi-Liang
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D029596:Paeonia / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers