title: Nuclear markers of Danube Sturgeons hybridization.
abstract: Acipenseriformes are composed of 25 sturgeon species and two paddlefish species distributed exclusively in the northern hemisphere. The Danube River and the Black Sea were originally inhabited by six sturgeon species but two are extinct and only four are still reproducing currently in the Lower Danube: Huso huso, Acipenser stellatus, A. gueldenstaedtii and A. ruthenus. Sturgeon species hybridize more easily than other fish and the determination of pure species or hybrid status is important for conservation and for breeding in fish farms. This survey demonstrated that morphological determination of this status is not reliable and a molecular tool, based on eight microsatellites genotypes is proposed. This method, based on three successive statistical analyses including Factorial Correspondence Analysis (FCA), Structure assignation and NewHybrids status determination, showed a high efficiency in discriminating pure species specimens from F1, F2 and two kinds of backcross individuals involving three of the four reproducing Lower Danube sturgeon species.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, Splaiul Independentei, Bucharest 91-95, 050095, Romania; E-Mails:  tn_andreea@yahoo.com  (A.D.);  georgescu_se@yahoo.com  (S.E.G.).
authors.forename: Andreea
authors.initials: A
authors.lastname: Dudu
authors.forename: Radu
authors.initials: R
authors.lastname: Suciu
authors.forename: Marian
authors.initials: M
authors.lastname: Paraschiv
authors.forename: Sergiu Emil
authors.initials: SE
authors.lastname: Georgescu
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.forename: Patrick
authors.initials: P
authors.lastname: Berrebi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D005399:Fishes / Q000378:metabolism; D005787:Gene Frequency; D056426:Genetic Loci; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D009693:Nucleic Acid Hybridization
chemical_list: D015415:Biomarkers
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