title: Alloantibodies against MHC class I: a novel mechanism of neonatal pancytopenia linked to vaccination.
abstract: Fetal/neonatal alloimmune thrombocytopenia is a frequent disease in humans where alloantibodies against platelet Ags lead to platelet destruction and hemorrhage. Although a role in the disease for Abs against MHC has been suspected, this has not been formally demonstrated. Since 2007, a hemorrhagic syndrome due to thrombocytopenia and designated as bovine neonatal pancytopenia (BNP) has been recognized in calves in several European countries. An inactivated antiviral vaccine is strongly suspected to be involved in this syndrome because of its highly frequent use in the dams of affected calves. In this study, we show that BNP is an alloimmune disease, as we reproduced the signs by transferring serum Abs from vaccinated BNP dams into healthy neonatal calves. Ab specificity was strongly associated with the presence of allogeneic MHC class I Abs in the dams. MHC class I staining was also observed on Madin-Darby bovine kidney cells, a cell line related to the one used to produce the vaccine Ag. Our report emphatically demonstrates that alloimmunization against MHC class I is associated with a substantial risk of developing cytopenia-associated syndromes in neonates when a cell line of the same species is used to produce an inactivated vaccine injected into the mother.
authors.affiliation: Université de Toulouse, Institut National Polytechnique de Toulouse, Ecole Nationale Vétérinaire de Toulouse, F-31076 Toulouse, France. g.foucras@envt.fr
authors.forename: Gilles
authors.initials: G
authors.lastname: Foucras
authors.forename: Fabien
authors.initials: F
authors.lastname: Corbière
authors.forename: Christian
authors.initials: C
authors.lastname: Tasca
authors.forename: Carole
authors.initials: C
authors.lastname: Pichereaux
authors.forename: Cécile
authors.initials: C
authors.lastname: Caubet
authors.forename: Catherine
authors.initials: C
authors.lastname: Trumel
authors.forename: Caroline
authors.initials: C
authors.lastname: Lacroux
authors.forename: Cyrielle
authors.initials: C
authors.lastname: Franchi
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
authors.forename: François
authors.initials: F
authors.lastname: Schelcher
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D005260:Female; D055785:Gene Knockdown Techniques; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000009:adverse effects; D007518:Isoantibodies / Q000008:administration & dosage / Q000009:adverse effects*; D007970:Leukopenia / Q000276:immunology; D010198:Pancytopenia / Q000276:immunology*; D013921:Thrombocytopenia / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000009:adverse effects
chemical_list: D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D007518:Isoantibodies; D015164:Vaccines, Inactivated; D014765:Viral Vaccines