title: Purification, crystallization and diffraction studies of the methyltransferases BT_2972 and BVU_3255 from antibiotic-resistant pathogens of the genus Bacteroides from the human intestine.
abstract: The methyltransferases BT_2972 and BVU_3255 from two different Bacteroides species that are antibiotic-resistant pathogens from the human intestine were cloned, overexpressed and purified, yielding approximately 120 mg of each protein from 1 l culture. Apo BT_2972 and BVU_3255 and their complexes with S-adenosylmethionine or S-adenosylhomocysteine were crystallized in four different crystal forms using the hanging-drop vapour-diffusion method. These crystals diffracted to resolutions ranging from 2.8 to 2.2 Å. Sequence analysis suggested that the two proteins are homologous small-molecule methyltransferases.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of Singapore.
authors.forename: Veerendra
authors.initials: V
authors.lastname: Kumar
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Mallika
authors.forename: J
authors.initials: J
authors.lastname: Sivaraman
mesh_terms: D001439:Bacteroides / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008780:Methyltransferases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D008780:Methyltransferases
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