title: A database of immunoglobulins with integrated tools: DIGIT.
abstract: The DIGIT (Database of ImmunoGlobulins with Integrated Tools) database (http://biocomputing.it/digit) is an integrated resource storing sequences of annotated immunoglobulin variable domains and enriched with tools for searching and analyzing them. The annotations in the database include information on the type of antigen, the respective germline sequences and on pairing information between light and heavy chains. Other annotations, such as the identification of the complementarity determining regions, assignment of their structural class and identification of mutations with respect to the germline, are computed on the fly and can also be obtained for user-submitted sequences. The system allows customized BLAST searches and automatic building of 3D models of the domains to be performed.
authors.affiliation: Department of Physics, Sapienza University of Rome, P.le A Moro 5-00185, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Chailyan
authors.forename: Anna
authors.initials: A
authors.lastname: Tramontano
authors.forename: Paolo
authors.initials: P
authors.lastname: Marcatili
mesh_terms: D030562:Databases, Protein; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics*; D058977:Molecular Sequence Annotation; D017421:Sequence Analysis; D012984:Software; D018511:Systems Integration; D009626:Terminology as Topic
chemical_list: D007135:Immunoglobulin Variable Region
references.citation: Nature. 1989 Dec 21-28;342(6252):877-83
references.pmid: 2687698
references.citation: Protein Eng. 1990 May;3(6):531-9
references.pmid: 2115164
references.citation: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D781-4
references.pmid: 16381979
references.citation: Methods. 2000 Mar;20(3):267-79
references.pmid: 10694450
references.citation: J Mol Biol. 1987 Aug 20;196(4):901-17
references.pmid: 3681981
references.citation: Proteins. 2011 May;79(5):1513-24
references.pmid: 21365679
references.citation: Bioinformatics. 2008 Sep 1;24(17):1953-4
references.pmid: 18641403
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D26-31
references.pmid: 18940867
references.citation: Proteins. 1992 Jul;13(3):231-45
references.pmid: 1603812
references.citation: J Mol Biol. 1998 Jan 16;275(2):269-94
references.pmid: 9466909
references.citation: FEBS J. 2011 Aug;278(16):2858-66
references.pmid: 21651726
references.citation: J Mol Biol. 1997 Nov 7;273(4):927-48
references.pmid: 9367782
references.citation: Nucleic Acids Res. 2005 Jan 1;33(Database issue):D671-4
references.pmid: 15608286
references.citation: Haematologica. 2010 Oct;95(10):1792-6
references.pmid: 20511668
references.citation: Nucleic Acids Res. 2001 Jan 1;29(1):205-6
references.pmid: 11125092
references.citation: J Clin Invest. 2004 Apr;113(7):1008-16
references.pmid: 15057307
references.citation: J Mol Biol. 2003 Jul 4;330(2):323-32
references.pmid: 12823971
references.citation: J Mol Biol. 1990 Sep 5;215(1):175-82
references.pmid: 2118959
references.citation: Mol Med. 2011;17(11-12):1188-95
references.pmid: 21785810
references.citation: Mol Immunol. 1995 Sep;32(13):1001-10
references.pmid: 7476997
references.citation: Biophys Chem. 1997 Oct;68(1-3):9-16
references.pmid: 9468606