title: Effects of auxins on sorgoleone accumulation and genes for sorgoleone biosynthesis in sorghum roots.
abstract: Sorgoleone is a major component of the hydrophobic root exudate of  Sorghum bicolor  and is of particular interest to plant chemical ecology as well as agriculture. Sorgoleone was evaluated in this study to observe the expression levels of genes involved in its biosynthesis in response to auxins. Sorgoleone content varied widely according to the duration of application and the concentrations of the auxins. When the application time was increased, the sorgoleone content increased accordingly for all concentrations of IBA (1, 3, and 5 mg/L) and at 1 mg/L for both IAA and NAA. In this study, five different sorgoleone biosynthetic genes were observed, namely DES2, DES3, ARS1, ARS2, and OMT3, which are upregulated in response to IAA, IBA, and NAA. Transcript accumulation was apparent for all genes, but particularly for DES2, which increased up to 475-fold and 180-fold following 72 h exposure to NAA and IBA, respectively, compared to no treatment.
authors.affiliation: Department of Crop Science, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Korea.
authors.forename: Md Romij
authors.initials: MR
authors.lastname: Uddin
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Park
authors.forename: Yong Kyoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Jong Yeong
authors.initials: JY
authors.lastname: Pyon
authors.forename: Sang-Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D016227:Benzoquinones; D015870:Gene Expression / Q000187:drug effects*; D006540:Herbicides; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis* / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D045868:Sorghum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D006540:Herbicides; D007210:Indoleacetic Acids; D008055:Lipids; C113738:sorgoleone