title: Expression of p97/VCP and ubiquitin during postnatal development of the degenerating rat retina.
abstract: In this study, we aimed to investigate the distribution pattern of ubiquitin and p97/VCP in the rat retina during postnatal development. Eyeballs from 1-, 4-, 10-, 36- and 72-week-old rats were examined by immunohistochemistry, and protein colocalization was determined by immunofluorescence microscopy. In the 1-week-old rat retina, p97/VCP was strongly expressed in the neuroblast layer, however no ubiquitin immunoreactivity was observed. p97/VCP immunoreactivity was present in the ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS) of the photoreceptor layer, and retinal pigment epithelium in the 4- and 10-week-old rat retinas. p97/VCP immunoreactivity increased significantly in the 10-week-old rat retinas. Ubiquitin was barely seen in the 4-week-old rat retinas, and ubiquitin expression was weak in the GCL and the IPL of the 10-week-old rat retinas. In the 36- and 72-week-old rats, the presence of ubiquitin was remarkable in the IS, INL, IPL and GCL, however, p97/VCP immunoreactivity was significantly decreased. Colocalization of ubiquitin and p97/VCP was also observed in the INL, IS, GCL and ONL of 36- and 72-week-old rat retinas. Our results indicate that p97/VCP immunoreactivity in the retina significantly decreases after rats reach 10 weeks of age, whereas ubiquitin immunoreactivity increases with aging. These results suggest that an altered expression pattern of p97/VCP and ubiquitin in the developing rat retina may associate with age-related retinal degeneration.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey. huseyin.ortak@hotmail.com
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ortak
authors.forename: Sevil
authors.initials: S
authors.lastname: Cayli
authors.forename: Ufuk
authors.initials: U
authors.lastname: Tas
authors.forename: Seda
authors.initials: S
authors.lastname: Ocakli
authors.forename: Erkan
authors.initials: E
authors.lastname: Söğüt
authors.forename: Helin Deniz
authors.initials: HD
authors.lastname: Demir
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D025801:Ubiquitin; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein; C000614180:Vcp protein, rat
references.citation: Acta Histochem. 2012 Jan;114(1):12-7
references.pmid: 21466889
references.citation: Nature. 1997 Jul 3;388(6637):75-8
references.pmid: 9214505
references.citation: Exp Gerontol. 2006 Nov;41(11):1067-79
references.pmid: 17052881
references.citation: Nat Cell Biol. 2001 Dec;3(12):1086-91
references.pmid: 11781570
references.citation: Neuroscience. 2008 Jun 26;154(3):1107-20
references.pmid: 18495355
references.citation: J Neuropathol Exp Neurol. 2011 Jan;70(1):63-8
references.pmid: 21157377
references.citation: Exp Gerontol. 2005 Aug-Sep;40(8-9):622-33
references.pmid: 16125351
references.citation: Mol Vis. 2010 Nov 18;16:2425-37
references.pmid: 21139979
references.citation: J Comp Neurol. 1997 Apr 21;380(4):449-71
references.pmid: 9087525
references.citation: Invest Ophthalmol Vis Sci. 2005 Mar;46(3):1033-8
references.pmid: 15728562
references.citation: Biochim Biophys Acta. 2010 Mar;1803(3):424-34
references.pmid: 20097236
references.citation: Curr Biol. 2005 Dec 6;15(23):R963-4
references.pmid: 16332527
references.citation: Postgrad Med J. 2006 Sep;82(971):581-7
references.pmid: 16954455
references.citation: Prog Retin Eye Res. 2010 May;29(3):169-90
references.pmid: 20206286
references.citation: EMBO J. 2000 May 15;19(10):2181-92
references.pmid: 10811609
references.citation: PLoS Genet. 2010 Aug 26;6(8):
references.pmid: 20865169
references.citation: Invest Ophthalmol Vis Sci. 1984 Feb;25(2):195-200
references.pmid: 6698741
references.citation: Autophagy. 2010 Feb;6(2):217-27
references.pmid: 20104022
references.citation: Eye (Lond). 1987;1 ( Pt 2):282-95
references.pmid: 3653437
references.citation: Genes Cells. 2007 Jul;12(7):889-901
references.pmid: 17584300
references.citation: Histochem Cell Biol. 2010 Jan;133(1):85-93
references.pmid: 19826831
references.citation: J Comp Neurol. 2004 Jun 21;474(2):304-24
references.pmid: 15164429
references.citation: Semin Cell Dev Biol. 2000 Jun;11(3):141-8
references.pmid: 10906270
references.citation: J Biol Chem. 2003 Mar 28;278(13):11579-89
references.pmid: 12509440
references.citation: Curr Opin Cell Biol. 1995 Apr;7(2):215-23
references.pmid: 7612274
references.citation: Mol Cell Biol. 2002 Jan;22(2):626-34
references.pmid: 11756557
references.citation: Mol Cell. 2001 Sep;8(3):499-504
references.pmid: 11583613
references.citation: Cell Death Differ. 2001 Oct;8(10):977-84
references.pmid: 11598795
references.citation: Mol Vis. 2010 Jan 11;16:7-17
references.pmid: 20069063
references.citation: Ageing Res Rev. 2002 Jun;1(3):465-79
references.pmid: 12067598
references.citation: Invest Ophthalmol Vis Sci. 1992 Jan;33(1):1-17
references.pmid: 1730530
references.citation: Invest Ophthalmol Vis Sci. 2009 Jul;50(7):3456-63
references.pmid: 19369235
references.citation: Ageing Res Rev. 2009 Jan;8(1):1-17
references.pmid: 18703168
references.citation: Ann N Y Acad Sci. 2007 Nov;1116:1-28
references.pmid: 18083918
references.citation: Nat Cell Biol. 2001 Aug;3(8):740-4
references.pmid: 11483959
references.citation: Invest Ophthalmol Vis Sci. 1993 Nov;34(12):3278-96
references.pmid: 8225863
references.citation: J Cell Biol. 2010 Sep 20;190(6):965-73
references.pmid: 20855502
references.citation: J Biol Chem. 2008 Sep 5;283(36):24561-70
references.pmid: 18614536
references.citation: Invest Ophthalmol Vis Sci. 1999 Sep;40(10):2398-404
references.pmid: 10476808
references.citation: Exp Biol Med (Maywood). 2011 Mar;236(3):268-76
references.pmid: 21383047