title: Charging of tRNAs using ribozymes and selection of cyclic peptides containing thioethers.
abstract: In vitro selection methods represent a powerful approach toward identifying high-affinity peptide ligands from highly diverse peptide libraries against a desired target. We herein describe a method for the display and selection of cyclic thioether peptide libraries. Reprogramming the initiation event from fMet to an N-chloroacetyl-amino acid by utilizing flexizyme to rapidly and efficiently prepare the aa-tRNA can be effectively used to initiate translation, upon which the thiol group of an inserted cysteine at the C terminus of the designed library spontaneously reacts to yield a nonreducible cyclic thioether peptide readily compatible with any in vitro display methods. Thus, cyclic peptides already in a nonreducible stable form can be selected directly against the target of interest.
authors.affiliation: PeptiDream Inc., Tokyo, Japan.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Reid
authors.forename: Yuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Katoh
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suga
mesh_terms: D019151:Peptide Library; D010456:Peptides, Cyclic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics; D013440:Sulfides / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D010456:Peptides, Cyclic; D016337:RNA, Catalytic; D013440:Sulfides; D012343:RNA, Transfer