title: Attaching and effacing Escherichia coli and Shiga toxin-producing E. coli in children with acute diarrhoea and controls in Teresina/PI, Brazil.
abstract: This 3.5-year prospective study was conducted to ascertain the level of attaching and effacing Escherichia coli (AEEC) associated diarrhoea in children from Teresina, a northeastern state of Brazil. Passed faecal specimens from 400 patients (250 with and 150 without diarrhoea) up to 60 months of age attending from 2004 to 2007 at two public hospitals were investigated. Conventional microbiology methods and PCR were employed. Escherichia coli was isolated from 390 children, 240 of them with diarrhoea. A total of 117 AEEC strains were cultivated from specimens from 63 children, 37 with and 26 without diarrhoea. No association between AEEC and diarrhoea was observed. Atypical enteropathogenic E. coli (a-EPEC) (79.4%) was more commonly found than typical EPEC (t-EPEC). Association between EPEC and EPEC subtypes and diarrhoea was not detected. Mixed infection by t-EPEC and a-EPEC and infection by Shiga toxin-producing E. coli (STEC) were rare. Enteropathogenic E. coli was more common in males and in children aged less than 12 months. Correlation between serotyping and PCR results was 0.19. High resistance rates of AEEC to ampicillin, cephalotin, and trimethoprim-sulfamethoxazole were found. In conclusion, EPEC is very common in children with diarrhoea and controls in the population we studied, with a-EPEC predominating. This diarrhoeagenic E. coli (DEC) pathotype is more common in infant males and is resistant to drugs frequently used in clinical practice.
authors.affiliation: Departamento de Propedêutica Complementar, Faculdade de Medicina, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, 30130-100, Belo Horizonte, MG, Brazil.
authors.forename: Maria do Rosário Conceição Moura
authors.initials: Mdo R
authors.lastname: Nunes
authors.forename: Paula Prazeres
authors.initials: PP
authors.lastname: Magalhães
authors.forename: Antônio da Silva
authors.initials: Ada S
authors.lastname: Macêdo
authors.forename: Roger Teixeira
authors.initials: RT
authors.lastname: Franco
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Penna
authors.forename: Edilberto Nogueira
authors.initials: EN
authors.lastname: Mendes
mesh_terms: D000208:Acute Disease; D018829:Adhesins, Bacterial / Q000235:genetics / Q000302:isolation & purification; D001422:Bacterial Adhesion / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000150:complications / Q000453:epidemiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D011446:Prospective Studies; D022621:Shiga Toxin / Q000302:isolation & purification*
chemical_list: D018829:Adhesins, Bacterial; C104901:BfpA protein, E coli; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli; D036781:Fimbriae Proteins; D022621:Shiga Toxin