title: Differences in the mechanism of inoculation between a semi-persistent and a non-persistent aphid-transmitted plant virus.
abstract: Inoculation of the semi-persistent cauliflower mosaic virus (CaMV, genus Caulimovirus) is associated with successive brief (5-10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. In contrast to non-persistent viruses, there is no evidence for which of the pd subphases (II-1, II-2 and II-3) is involved in the inoculation of CaMV. Experiments were conducted using the electrical penetration graph (EPG) technique to investigate which particular subphases of the pd are associated with the inoculation of CaMV to turnip by its aphid vector Brevicoryne brassicae. In addition, the same aphid species/test plant combination was used to compare the role of the pd subphases in the inoculation of the non-persistent turnip mosaic virus (TuMV, genus Potyvirus). Inoculation of TuMV was found to be related to subphase II-1, confirming earlier results, but CaMV inoculation appeared to be related exclusively to subphase II-2 instead. The mechanism of CaMV inoculation and the possible nature of subphase II-2 are discussed in the scope of our findings.
authors.affiliation: Departamento de Protección Vegetal, Instituto de Ciencias Agrarias, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain.
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Moreno
authors.affiliation: EPG systems, Dillenburg 12, 6703 CJ Wageningen, The Netherlands.|Laboratory of Entomology, Wageningen University, PO Box 8031, 6700 EH Wageningen, The Netherlands.
authors.forename: W Freddy
authors.initials: WF
authors.lastname: Tjallingii
authors.affiliation: Departamento de Protección Vegetal, Instituto de Ciencias Agrarias, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Fernandez-Mata
authors.affiliation: Departamento de Protección Vegetal, Instituto de Ciencias Agrarias, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Fereres
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology / Q000821:virology*; D029688:Brassica napus / Q000821:virology*; D017796:Caulimovirus / Q000472:pathogenicity*; D017800:Potyvirus / Q000472:pathogenicity*; D053586:Virus Internalization