title: Glycan-modifying bacteria-derived soluble factors from Bacteroides thetaiotaomicron and Lactobacillus casei inhibit rotavirus infection in human intestinal cells.
abstract: Rotaviruses attach to intestinal cells in a process that requires glycan recognition. Some bacteria from the gut microflora have been shown to modify cell-surface glycans. In this study, human intestinal cultured cells were incubated with bacteria-derived soluble factors and infected with rotavirus. Results show that only bacterial soluble factors that increase cell-surface galactose namely, those of Bacteroides thetaiotaomicron and Lactobacillus casei were able to efficiently block rotavirus infections. Increasing cell-surface galactose using galactosyltransferase resulted in a similar blockage of rotavirus infections. These results indicate that manipulation of cell-surface intestinal glycans by bacterial soluble factors can prevent rotavirus infection in a species-specific manner, and should now be considered a potential therapeutic approach against rotavirus infection.
authors.affiliation: UPMC Univ Paris 06, ERL INSERM U1057/UMR7203, Faculty of Medicine Pierre et Marie Curie, CHU Saint-Antoine, 27 rue de Chaligny 75012 Paris, France.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Varyukhina
authors.forename: Miguel
authors.initials: M
authors.lastname: Freitas
authors.forename: Sabine
authors.initials: S
authors.lastname: Bardin
authors.forename: Emilie
authors.initials: E
authors.lastname: Robillard
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Tavan
authors.forename: Catherine
authors.initials: C
authors.lastname: Sapin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Grill
authors.forename: Germain
authors.initials: G
authors.lastname: Trugnan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D001439:Bacteroides / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D005700:Galactosyltransferases / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D007422:Intestines / Q000187:drug effects / Q000821:virology; D007780:Lacticaseibacillus casei / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding; D012401:Rotavirus / Q000187:drug effects* / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000188:drug therapy / Q000517:prevention & control*; D012995:Solubility; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011134:Polysaccharides; D005700:Galactosyltransferases; D005690:Galactose