title: Sequential injection immunoassay for environmental measurements.
abstract: Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described.  A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve.  We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems.  For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed.  On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO.  For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed.  These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.
authors.affiliation: Faculty of Agriculture, Saga University, Saga, Japan. nsoh@cc.saga-u.ac.jp
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Soh
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tanaka
authors.forename: Koji
authors.initials: K
authors.lastname: Hirakawa
authors.forename: RuiQi
authors.initials: R
authors.lastname: Zhang
authors.forename: Hizuru
authors.initials: H
authors.lastname: Nakajima
authors.forename: Koji
authors.initials: K
authors.lastname: Nakano
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Imato
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D014819:Vitellogenins / Q000032:analysis* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D014819:Vitellogenins; D014874:Water Pollutants, Chemical