title: Comparison of anti-atherosclerotic effects of two different extracts from leaves of Mallotus furetianus.
abstract: OBJECTIVE
  To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus furetianus by using rat model of atherosclerosis.
  
  
  METHODS
  The air-dried powdered Mallotus furetianus leaves were extracted with ethanol and then evaporated. The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H2O (50:50, 100:0, v/v) and two fractions, Mallotus furetianus A (Mf A) and Mallotus furetianus B (Mf B) were obtained. Rats were divided into control, atherosclerosis and vitamin E, Mf A and Mf B treated groups. Atherosclerotic model was established by administering a loading dose of vitamin D3 and feeding standard diet enriched with 2% cholesterol, 0.5% porcine cholate, 0.2% methimazole, 5% sugar, 10% pork fat. Vitamin E (0.20 g/kg), Mf A (0.053 g/kg), Mf B (0.057 g/kg) (with the potential) were administered to interfere with the development of atherosclerosis. After 9 weeks, rats were sacrificed and the blood lipid as well as composition of bile was examined. In addition, the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.
  
  
  RESULTS
  Atherosclerosis model was successfully established, administration of vitamin E, Mf A and Mf B increased excretion of total bilirubin in bile, decreased triglyeride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) level, enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood, improved histological changes and diminished intima-media thickness ratio of thoracic aorta in atherosclerotic rats. As for the difference in anti-atherosclerotic effects betweenMf A and Mf B, Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.
  
  
  CONCLUSIONS
  The two different extracts, Mf A and Mf B can prevent the development of atherosclerosis, In detail, Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC level in atherosclerotic rats.
authors.affiliation: School of Pharmacy, Hainan Medical College, Haikou 571101, P. R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yueli
authors.forename: Wang
authors.initials: W
authors.lastname: Liqun
authors.forename: Wu
authors.initials: W
authors.lastname: Haitao
authors.forename: Lin
authors.initials: L
authors.lastname: Lianbo
authors.forename: Yi
authors.initials: Y
authors.lastname: Xinan
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology; D050197:Atherosclerosis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D001646:Bile / Q000737:chemistry; D001663:Bilirubin / Q000032:analysis; D016022:Case-Control Studies; D002762:Cholecalciferol; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004195:Disease Models, Animal; D036461:Mallotus Plant / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D010936:Plant Extracts; D014280:Triglycerides; D002762:Cholecalciferol; D002784:Cholesterol; D001663:Bilirubin