title: Effectiveness of AFLPs and retrotransposon-based markers for the identification of Portuguese grapevine cultivars and clones.
abstract: Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.
authors.affiliation: University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), Apartado 1013, 5001-801 Vila Real, Portugal. icastro@utad.pt
authors.forename: Isaura
authors.initials: I
authors.lastname: Castro
authors.forename: Claudio
authors.initials: C
authors.lastname: D'Onofrio
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Martín
authors.forename: Jesús María
authors.initials: JM
authors.lastname: Ortiz
authors.forename: Gabriella
authors.initials: G
authors.lastname: De Lorenzis
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ferreira
authors.forename: Olinda
authors.initials: O
authors.lastname: Pinto-Carnide
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D002999:Clone Cells; D016000:Cluster Analysis; D060146:Ecotype; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011174:Portugal; D018626:Retroelements / Q000235:genetics*; D027843:Vitis / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D018626:Retroelements
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