title: Inhibition of recombinant human carboxylesterase 1 and 2 and monoacylglycerol lipase by chlorpyrifos oxon, paraoxon and methyl paraoxon.
abstract: Oxons are the bioactivated metabolites of organophosphorus insecticides formed via cytochrome P450 monooxygenase-catalyzed desulfuration of the parent compound. Oxons react covalently with the active site serine residue of serine hydrolases, thereby inactivating the enzyme. A number of serine hydrolases other than acetylcholinesterase, the canonical target of oxons, have been reported to react with and be inhibited by oxons. These off-target serine hydrolases include carboxylesterase 1 (CES1), CES2, and monoacylglycerol lipase. Carboxylesterases (CES, EC 3.1.1.1) metabolize a number of xenobiotic and endobiotic compounds containing ester, amide, and thioester bonds and are important in the metabolism of many pharmaceuticals. Monoglyceride lipase (MGL, EC 3.1.1.23) hydrolyzes monoglycerides including the endocannabinoid, 2-arachidonoylglycerol (2-AG). The physiological consequences and toxicity related to the inhibition of off-target serine hydrolases by oxons due to chronic, low level environmental exposures are poorly understood. Here, we determined the potency of inhibition (IC(50) values; 15 min preincubation, enzyme and inhibitor) of recombinant CES1, CES2, and MGL by chlorpyrifos oxon, paraoxon and methyl paraoxon. The order of potency for these three oxons with CES1, CES2, and MGL was chlorpyrifos oxon>paraoxon>methyl paraoxon, although the difference in potency for chlorpyrifos oxon with CES1 and CES2 did not reach statistical significance. We also determined the bimolecular rate constants (k(inact)/K(I)) for the covalent reaction of chlorpyrifos oxon, paraoxon and methyl paraoxon with CES1 and CES2. Consistent with the results for the IC(50) values, the order of reactivity for each of the three oxons with CES1 and CES2 was chlorpyrifos oxon>paraoxon>methyl paraoxon. The bimolecular rate constant for the reaction of chlorpyrifos oxon with MGL was also determined and was less than the values determined for chlorpyrifos oxon with CES1 and CES2 respectively. Together, the results define the kinetics of inhibition of three important hydrolytic enzymes by activated metabolites of widely used agrochemicals.
authors.affiliation: Center for Environmental Health Sciences, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: J Allen
authors.initials: JA
authors.lastname: Crow
authors.forename: Victoria
authors.initials: V
authors.lastname: Bittles
authors.forename: Katye L
authors.initials: KL
authors.lastname: Herring
authors.forename: Abdolsamad
authors.initials: A
authors.lastname: Borazjani
authors.forename: Philip M
authors.initials: PM
authors.lastname: Potter
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Ross
mesh_terms: D043182:Carboxylesterase / Q000037:antagonists & inhibitors*; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D004390:Chlorpyrifos / Q000031:analogs & derivatives* / Q000633:toxicity; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008994:Monoacylglycerol Lipases / Q000037:antagonists & inhibitors*; D010261:Paraoxon / Q000031:analogs & derivatives* / Q000633:toxicity*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors
chemical_list: D007306:Insecticides; D011994:Recombinant Proteins; C009618:O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate; D002265:Carboxylic Ester Hydrolases; C474609:CES1 protein, human; C517204:CES2 protein, human; D043182:Carboxylesterase; D008994:Monoacylglycerol Lipases; D004390:Chlorpyrifos; D010261:Paraoxon; C008480:methylparaoxon
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