title: Effects of cryopreservation and/or decellularization on extracellular matrix of porcine valves.
abstract: The objective of this study was to evaluate the morphology of decellularized and/or cryopreserved porcine pulmonary valves, to determine a solution capable of completely remove the cells without damaging the extracellular matrix. Porcine pulmonary valves were incubated for 24 hs in sodium deoxicholate 1% or sodium dodecyl sulfate 0.1 and 0.3%, with or without associated cryopreservation. Evaluation was done with optical microscopy (Hematoxilin-Eosin, Acetic Orcein and Gomori) and with morphometric analysis. The effectiveness of the solutions was variable, but the best results were obtained with the sodium dodecyl sulfate solution 0.1%.
authors.affiliation: Pontifícia Universidade Católica do Paraná/Cardioprótese Ltda, Curitiba, PR, Brasil.
authors.forename: Luciana Cristina Ferretti de Nazareno
authors.initials: LC
authors.lastname: Wollmann
authors.forename: Carlos A H
authors.initials: CA
authors.lastname: Laurindo
authors.forename: Francisco Diniz Affonso da
authors.initials: FD
authors.lastname: Costa
authors.forename: Andréa Novais
authors.initials: AN
authors.lastname: Moreno
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005109:Extracellular Matrix / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D006350:Heart Valve Prosthesis; D011664:Pulmonary Valve / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012996:Solutions; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D012996:Solutions; D012967:Sodium Dodecyl Sulfate