title: Diverse H. pylori strains, IL-10 promoter polymorphisms with high morbidity of gastric cancer in Hexi area of Gansu Province, China.
abstract: In Hexi area of Gansu Province, people have a higher susceptibility of gastric cancer than people in the rest area of China. There is substantial geographic variation in the incidence of gastric cancer. In this article, the present author explored the roles of H. pylori infection and IL-10 promoter polymorphisms in development of gastric cancer in this area. A total of 304 participants were admitted to our study, and they were divided into two groups: control group and case group. Blood samples from all subjects were collected for gene extraction using DNA extraction kits. IL-10 polymorphisms were determined by SNaPshot Multiplex. To test H. pylori infection and its typing H. pylori antibody Immunoblotting Kits were used. This research suggested that environmental factor played an important role in the pathogenesis of gastric carcinoma in the area, H. pylori infection increased the risk of gastric cancer (OR = 2.612, 95% CI 1.636-4.170) and subject with H. pylori I-type positive was at significantly higher risk for progression to gastric cancer (OR = 4.712, 95% CI 2.656-8.537). For subjects with the ATA/GCC or GCC/GCC haplotype of the IL-10-1082/-819/-592 polymorphism relative to the ATA/ATA haplotype group, the risk of gastric cancer development was significantly increased. It has been demonstrated that the presence of IL-10-819 C alleles and IL-10-592 C alleles was associated with an increased risk for gastric cancer development in H. pylori-infected patients and IL-10 promoter polymorphisms and H. pylori have a synergistic effect on gastric cancer in Hexi population.
authors.affiliation: Key Laboratory of Gastrointestinal Cancer of Gansu Province, The Second Hospital of Lanzhou University, Lanzhou, Gansu Province, People's Republic of China. zengxt2010@lzu.cn
authors.forename: Xiangting
authors.initials: X
authors.lastname: Zeng
authors.forename: Yumin
authors.initials: Y
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Junqiang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000382:microbiology
chemical_list: D016753:Interleukin-10
references.citation: Rev Physiol Biochem Pharmacol. 2004;152:205-20
references.pmid: 15549607
references.citation: J Clin Invest. 2001 Apr;107(7):767-73
references.pmid: 11285290
references.citation: Gut. 1998 Jul;43 Suppl 1:S6-9
references.pmid: 9764031
references.citation: Scand J Gastroenterol. 2002 Aug;37(8):891-8
references.pmid: 12229962
references.citation: J Infect Dis. 2002 Jan 1;185(1):106-9
references.pmid: 11756988
references.citation: Gut. 2004 Aug;53(8):1082-9
references.pmid: 15247172
references.citation: Cancer Lett. 2005 Jul 28;225(2):207-14
references.pmid: 15978325
references.citation: Dig Dis Sci. 2003 Jan;48(1):36-46
references.pmid: 12645788
references.citation: J Gastroenterol. 2004;39(3):220-30
references.pmid: 15064998
references.citation: Dig Dis. 2001;19(2):99-103
references.pmid: 11549817
references.citation: Cancer Causes Control. 2006 Feb;17(1):117-25
references.pmid: 16411061
references.citation: Gastroenterology. 2003 Dec;125(6):1636-44
references.pmid: 14724815
references.citation: Nat Rev Cancer. 2002 Jan;2(1):28-37
references.pmid: 11902583
references.citation: Eur J Immunogenet. 1997 Feb;24(1):1-8
references.pmid: 9043871
references.citation: Intern Med. 2008;47(12):1077-83
references.pmid: 18552463
references.citation: Am J Epidemiol. 2010 Aug 15;172(4):397-406
references.pmid: 20647333
references.citation: Am J Respir Crit Care Med. 2003 Aug 15;168(4):476-80
references.pmid: 12746253
references.citation: Am J Physiol. 1994 Dec;267(6 Pt 1):G966-71
references.pmid: 7810664
references.citation: Scand J Gastroenterol. 2001 Feb;36(2):208-13
references.pmid: 11252415
references.citation: Am J Gastroenterol. 1999 Dec;94(12):3455-9
references.pmid: 10606302
references.citation: Mol Biol Rep. 2009 Jul;36(6):1461-7
references.pmid: 18716896
references.citation: Eur J Epidemiol. 2011 Jan;26(1):23-8
references.pmid: 21086022
references.citation: Nat Rev Microbiol. 2005 Apr;3(4):320-32
references.pmid: 15759043
references.citation: Nat Rev Cancer. 2004 Sep;4(9):688-94
references.pmid: 15343275
references.citation: Cytokine. 2005 Feb 21;29(4):141-52
references.pmid: 15652446
references.citation: Cancer Res. 1992 Dec 15;52(24):6735-40
references.pmid: 1458460
references.citation: Blood. 1999 Dec 1;94(11):3941-6
references.pmid: 10572111
references.citation: Cell. 2002 Sep 20;110(6):673-87
references.pmid: 12297042
references.citation: Cancer Res. 1995 May 15;55(10):2111-5
references.pmid: 7743510
references.citation: CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
references.pmid: 15761078
references.citation: Lancet. 1993 May 29;341(8857):1359-62
references.pmid: 8098787
references.citation: Lancet. 2009 Nov 7;374(9701):1594; author reply 1594-5
references.pmid: 19897123
references.citation: N Engl J Med. 2002 Oct 10;347(15):1175-86
references.pmid: 12374879
references.citation: Helicobacter. 2009 Apr;14(2):81-6
references.pmid: 19298334
references.citation: Lancet. 2003 Oct 11;362(9391):1231-3
references.pmid: 14568748
references.citation: Gastroenterology. 2005 May;128(5):1229-42
references.pmid: 15887107
references.citation: J Gastroenterol Hepatol. 2007 Sep;22(9):1443-9
references.pmid: 17716348
references.citation: World J Gastroenterol. 2006 Jan 21;12(3):354-62
references.pmid: 16489633
references.citation: Gastroenterology. 2003 May;124(5):1193-201
references.pmid: 12730860
references.citation: Curr Opin Cell Biol. 2006 Oct;18(5):516-23
references.pmid: 16919435
references.citation: Lancet. 2003 Jul 26;362(9380):305-15
references.pmid: 12892963
references.citation: Eur J Cancer Prev. 2011 Mar;20(2):132-9
references.pmid: 21160428
references.citation: Ai Zheng. 2008 Jan;27(1):35-40
references.pmid: 18184461