title: Genomic DNA extraction from medicinal plants available in Malaysia using a TriOmic(TM) improved extraction kit.
abstract: DNA extraction  was carried out on 32 medicinal plant samples available in Malaysia using the  TriOmic(TM) extraction kit. Amounts of 0.1 g  flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1(plus) buffer  and followed by RNAse treatment. Then, RY2 buffer was added to the samples and  mixed completely by vortexing before removal of cell debris by centrifugation.  Supernatants were transferred to fresh microcentrifuge tubes and 0.1 volume RY3  buffer was added to each of the transferred supernatant. The mixtures were  applied to spin columns followed by a centrifugation step to remove buffers and  other residues. Washing step was carried out twice by applying 70% ethanol to  the spin columns. Genomic DNA of the samples was recovered by applying 50 μL TE buffer to the membrane of each  spin column, followed by a centrifugation step at room temperature. A  modification of the TriOmic(TM) extraction  procedure was carried out by adding chloroform:isoamyl alcohol (24:1) steps in  the extraction procedure. The genomic DNA extracted from most of the 32 samples  showed an increase of total yield when chloroform:isoamyl alcohol (24:1) steps  were applied in the TriOmicTM extraction  procedure. This preliminary study is very important for molecular studies of  medicinal plants available in Malaysia since the DNA extraction can be  completed in a shorter period of time (within 1 h) compared to manual  extraction, which entails applying phenol, chloroform and ethanol  precipitation, and requires 1-2 days to complete.
authors.affiliation: Department of Cell and Molecular Biology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: A R
authors.initials: AR
authors.lastname: Mohd-Hairul
authors.forename: A B
authors.initials: AB
authors.lastname: Sade
authors.forename: B C
authors.initials: BC
authors.lastname: Yiap
authors.forename: A R
authors.initials: AR
authors.lastname: Raha
mesh_terms: D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D008296:Malaysia; D010946:Plants, Medicinal / Q000737:chemistry*; D011933:Reagent Kits, Diagnostic
chemical_list: D018744:DNA, Plant; D011933:Reagent Kits, Diagnostic