title: Redox remodeling by dendritic cells protects antigen-specific T cells against oxidative stress.
abstract: Microorganisms and microbial products induce the release of reactive oxygen species (ROS) from monocytes and other myeloid cells, which may trigger dysfunction and apoptosis of adjacent lymphocytes. Therefore, T cell-mediated immunity is likely to comprise mechanisms of T cell protection against ROS-inflicted toxicity. The present study aimed to clarify the dynamics of reduced sulfhydryl groups (thiols) in human T cells after presentation of viral and bacterial Ags by dendritic cells (DCs) or B cells. DCs, but not B cells, efficiently triggered intra- and extracellular thiol expression in T cells with corresponding Ag specificity. After interaction with DCs, the Ag-specific T cells acquired the capacity to neutralize exogenous oxygen radicals and resisted ROS-induced apoptosis. Our results imply that DCs provide Ag-specific T cells with antioxidative thiols during Ag presentation, which suggests a novel aspect of DC/T cell cross-talk of relevance to the maintenance of specific immunity in inflamed or infected tissue.
authors.affiliation: Sahlgrenska Cancer Center, University of Gothenburg, 413 90 Gothenburg, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Martner
authors.forename: Johan
authors.initials: J
authors.lastname: Aurelius
authors.forename: Anna
authors.initials: A
authors.lastname: Rydström
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Hellstrand
authors.forename: Fredrik B
authors.initials: FB
authors.lastname: Thorén
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000382:microbiology / Q000821:virology; D002450:Cell Communication / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000382:microbiology / Q000821:virology; D002478:Cells, Cultured; D018920:Coculture Techniques; D003587:Cytomegalovirus / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D004768:Enterotoxins / Q000276:immunology / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006801:Humans; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000276:immunology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology / Q000821:virology
chemical_list: D004768:Enterotoxins; D018984:Epitopes, T-Lymphocyte; D013438:Sulfhydryl Compounds; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal