title: Fabrication of a chitosan/glucose oxidase-poly(anilineboronic acid)-Au(nano)/Au-plated Au electrode for biosensor and biofuel cell.
abstract: Enzyme immobilization is one of the key factors in constructing high-performance enzyme biosensors and biofuel cells (BFCs). Herein, we propose a new protocol for efficient immobilization of a glycoprotein enzyme based on the interaction of the 1, 2- or 1, 3-diols in the glycoprotein with a boronic acid functionalized monomer. Briefly, casting a mixture of glucose oxidase (GOx) and anilineboronic acid (ABA) followed by a NaAuCl(4) solution to an Au-plated Au electrode surface yielded a GOx-poly(ABA) (PABA)-gold nanoparticle (Au(nano)) bionanocomposite, and chitosan (CS) was then cast and air-dried. In the present protocol, the small-sized Au(nano) or Au subnanostructures can form near/on the enzyme molecule, which greatly promotes the electron transfer of enzymatic reaction and enhances the amperometric responses. The thus-prepared CS/GOx-PABA-Au(nano)/Au-plated Au electrode worked well in the first-/second generation biosensing modes and as a bioanode in a monopolar biofuel cell, with analytical or cell-power performance superior to those of most analogues hitherto reported.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.forename: Zou
authors.initials: Z
authors.lastname: Li
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Fu
authors.forename: Cong
authors.initials: C
authors.lastname: Qin
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Su
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Qingji
authors.initials: Q
authors.lastname: Xie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation*; D001897:Boronic Acids / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D001897:Boronic Acids; C416807:polyaniline; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose