title: Identification and phylogenetic characterization of a new subfamily of α-amylase enzymes from marine microorganisms.
abstract: A gene encoding a starch-hydrolyzing enzyme was isolated from a marine metagenomic library and overexpressed in Escherichia coli. The enzyme, designated AmyP, shows very low similarity to full-length sequences of known α-amylases, although a catalytic domain correlated with the α-amylase superfamily was identified. Based on the range of substrate hydrolysis and the product profile, the protein was clearly defined as a saccharifying-type α-amylase. Sequence comparison indicated that AmyP was related to four putative glycosidases previously identified only in bacterial genome sequences. They were all from marine bacteria and formed a new subfamily of glycoside hydrolase GH13. Moreover, this subfamily was closely related to the probable genuine bacterial α-amylases (GH13_19). The results suggested that the subfamily may be an independent clade of ancestral marine bacterial α-amylases.
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, Anhui Province, School of Life Sciences, Anhui University, Hefei 230039 Anhui, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Lei
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004789:Enzyme Activation; D004795:Enzyme Stability; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010802:Phylogeny; D013379:Substrate Specificity; D014871:Water Microbiology; D000516:alpha-Amylases / Q000737:chemistry* / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D000516:alpha-Amylases
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