title: Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells.
abstract: Antigen-IgE-mediated mucosal mast-cell activation is critical in the development of food allergies. Cinnamaldehyde, a major constituent of Cinnamomi cortex, dose-dependently inhibited the antigen-IgE-induced degranulation of mucosal-type bone-marrow derived mast cells (mBMMCs) and RBL-2H3 cells. Cinnamaldehyde also suppressed the elevation of the intracellular Ca(2+) level that is induced by the extracellular Ca(2+) influx in antigen-IgE-stimulated mBMMCs. Furthermore, tyrosine phosphorylation of phospholipase C (PLC) γ1, which is a crucial activation switch for the intracellular Ca(2+) mobilization in mast cells, was attenuated by cinnamaldehyde. Together, our results demonstrated that cinnamaldehyde suppressed the intracellular Ca(2+) mobilization and the degranulation of mucosal mast cells by inhibiting the activity of the IgE receptor-PLCγ-Ca(2+) influx pathway. These findings suggest that cinnamaldehyde may have therapeutic potential in mucosal mast cell-related allergic diseases, such as food allergies.
authors.affiliation: Division of Gastrointestinal Pathophysiology, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kageyama-Yahara
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Katagiri
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.forename: Makoto
authors.initials: M
authors.lastname: Kadowaki
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000941:Antigens / Q000276:immunology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects*; D002478:Cells, Cultured; D027424:Cinnamomum / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D051966:Phospholipase C gamma / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects
chemical_list: D000941:Antigens; D000972:Antineoplastic Agents, Phytogenic; D000171:Acrolein; D051966:Phospholipase C gamma; C012843:cinnamaldehyde; D002118:Calcium