title: Extracellular hsp70 release in canine Steroid Responsive Meningitis-Arteritis.
abstract: The role of extracellular 70 kDa heat shock protein 70 (ehsp70) in central nervous system inflammation is vastly understudied, despite evidence supporting the ability to drive a pro-inflammatory state. We investigated the presence of ehsp70 in cerebrospinal fluid (CSF) and serum of dogs with Steroid Responsive Meningitis-Arteritis (SRMA), with the hypothesis that an ehsp70 response would occur, and might play a role in the pathogenesis of this disease. Samples from 30 dogs acutely affected with SRMA, and 30 dogs treated with corticosteroids and currently in clinical remission from SRMA were compared with normal dogs. Serum and CSF concentrations of ehsp70 were quantified using an enzyme-linked immunosorbent assay. An ehsp70 response occurred in the CSF of dogs with SRMA and this response was attenuated by corticosteroid treatment. There was no correlation between serum and CSF concentrations of ehsp70, supporting local production and release of ehsp70 and not simply leakage from serum. Dogs with SRMA thus represent a powerful spontaneous model by which to study the role of ehsp70 in CNS inflammation.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp. St., Columbus, OH 43210, USA. moore.2204@osu.edu
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Moore
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Kim
authors.forename: Arianna
authors.initials: A
authors.lastname: Maiolini
authors.forename: Andrea
authors.initials: A
authors.lastname: Tipold
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Oglesbee
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000818:Animals; D001167:Arteritis / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000134:cerebrospinal fluid*; D007070:Immunoglobulin A / Q000097:blood / Q000134:cerebrospinal fluid; D007958:Leukocyte Count / Q000662:veterinary; D008581:Meningitis / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*
chemical_list: D000305:Adrenal Cortex Hormones; D018840:HSP70 Heat-Shock Proteins; D007070:Immunoglobulin A
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