title: In vitro effects of some herbs used in Egyptian traditional medicine on viability of protoscolices of hydatid cysts.
abstract: The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 µg/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 µg/ml) were used. Concentration of 2,500 µg/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 µg/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 µg/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 µg/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.
authors.affiliation: Department of Medical Parasitology, Assiut University, Egypt. doaayones@gmail.com
authors.forename: Doaa A
authors.initials: DA
authors.lastname: Yones
authors.forename: Gamal A
authors.initials: GA
authors.lastname: Taher
authors.forename: Zedan Z
authors.initials: ZZ
authors.lastname: Ibraheim
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D002162:Camelus; D002470:Cell Survival / Q000187:drug effects; D004445:Echinococcosis, Pulmonary / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000187:drug effects* / Q000302:isolation & purification; D008168:Lung / Q000469:parasitology; D008519:Medicine, Traditional / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D027543:Salvia officinalis / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry; D013997:Time Factors
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts
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