title: Monoclonal immunoglobulin protein production in two dogs with secretory B-cell lymphoma with mott cell differentiation.
abstract: METHODS
  A 9-year-old castrated male mixed-breed dog and a 7-year-old spayed female Boston Terrier, with clinical histories of a liver mass (dog 1) and bloody vomitus, diarrhea, and weight loss (dog 2), respectively, were referred for further evaluation.
  
  
  RESULTS
  At the time of referral, each dog had differing laboratory abnormalities; however, the serum total protein and globulin concentrations were within reference range in both dogs. Cytologic examination of fine-needle aspirates obtained from affected organs (a liver mass [dog 1] and enlarged submandibular lymph node [dog 2]) revealed 2 main nucleated cell types: atypical lymphoid cells and lesser numbers of Mott cells. With the use of serum immunofixation electrophoresis and serum immunoglobulin quantification, a monoclonal immunoglobulin protein was identified in both dogs and a final diagnosis of secretory B-cell lymphoma with Mott cell differentiation (MCL) was made.
  
  
  RESULTS
  Both dogs received chemotherapy for their disease. The first dog was euthanized 8.5 months after diagnosis because of acute respiratory distress of unknown etiology, and the second was euthanized 7 days after diagnosis for worsening clinical disease and quality of life.
  
  
  CONCLUSIONS
  To our knowledge, this report is the first of a secretory form of MCL in dogs. Findings indicate that in dogs with suspect MCL, even in patients that lack characteristic hyperproteinemia or hyperglobulinemia, serum protein content should be fully evaluated for the presence of a monoclonal immunoglobulin protein. Such an evaluation that uses immunofixation electrophoresis and immunoglobulin quantification will aid in the diagnosis of MCL in dogs.
authors.affiliation: Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. davis.seelig@colostate.edu
authors.forename: Davis M
authors.initials: DM
authors.lastname: Seelig
authors.forename: James A
authors.initials: JA
authors.lastname: Perry
authors.forename: Karen
authors.initials: K
authors.lastname: Zaks
authors.forename: Anne C
authors.initials: AC
authors.lastname: Avery
authors.forename: Paul R
authors.initials: PR
authors.lastname: Avery
mesh_terms: D000818:Animals; D044963:Biopsy, Fine-Needle / Q000662:veterinary; D002454:Cell Differentiation; D004283:Dog Diseases / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D017809:Fatal Outcome; D005260:Female; D007136:Immunoglobulins / Q000378:metabolism*; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008297:Male
chemical_list: D007136:Immunoglobulins