title: Collection of peripheral blood CD34+ progenitor cells from healthy dogs and dogs diagnosed with lymphoproliferative diseases using a Baxter-Fenwal CS-3000 Plus blood cell separator.
abstract: BACKGROUND
  Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter-Fenwal CS-3000 Plus automated blood cell separator has not been reported.
  
  
  OBJECTIVE
  To determine the feasibility and safety of using a CS-3000 Plus blood cell separator with a small volume separation container holder (SVSCH) and small volume collection chamber (SVCC) to harvest canine PBMCs from dogs weighing <50 kg.
  
  
  METHODS
  Eight healthy mongrel dogs and 11 client-owned dogs in clinical remission for lymphoproliferative diseases (LPD).
  
  
  METHODS
  In this prospective study, aphereses were performed using a Baxter-Fenwal CS-3000 Plus blood cell separator, with or without recombinant human granulocyte colony-stimulating factor (rhG-CSF) treatment.
  
  
  RESULTS
  Aphereses from 6 healthy dogs given rhG-CSF yielded an average of 1.1 × 10(7) ± 8.2 × 10(6) CD34+ cells/kg. Aphereses from LPD dogs given rhG-CSF yielded an average of 5.4 × 10(6) ± 3.25 × 10(6) CD34+ cells/kg (P = .17). Higher hematocrit in both groups of dogs receiving rhG-CSF correlated with an increased number of CD34+ cells/kg harvested (healthy, P = .04; LPD, P = .05). Apheresis was well tolerated by all dogs.
  
  
  CONCLUSIONS
  Canine PBMC apheresis using the Baxter-Fenwal CS-3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation.
authors.affiliation: College of Veterinary Medicine Teaching Hospital, North Carolina State University, Raleigh, NC 27606, USA. steven_suter@ncsu.edu
authors.forename: S E
authors.initials: SE
authors.lastname: Suter
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D007937:Leukapheresis / Q000295:instrumentation / Q000662:veterinary*; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D015203:Reproducibility of Results
chemical_list: D018952:Antigens, CD34