title: Protease-activated receptor 2 is involved in Th2 responses against Trichinella spiralis infection.
abstract: In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.
authors.affiliation: Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-870, Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Cho
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kang
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Park
authors.forename: Yun Seong
authors.initials: YS
authors.lastname: Kim
authors.forename: Ki Uk
authors.initials: KU
authors.lastname: Kim
authors.forename: Soon Cheol
authors.initials: SC
authors.lastname: Ahn
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Hak Sun
authors.initials: HS
authors.lastname: Yu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D054413:Chemokine CCL11 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D020869:Gene Expression Profiling; D007073:Immunoglobulin E / Q000097:blood; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D007378:Interleukins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D044464:Receptor, PAR-2 / Q000378:metabolism*; D018418:Th2 Cells / Q000276:immunology*; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D000909:Antibodies, Helminth; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D016207:Cytokines; D018793:Interleukin-13; D015848:Interleukin-5; D007378:Interleukins; C442281:Mydgf protein, mouse; D044464:Receptor, PAR-2; D015847:Interleukin-4; D007073:Immunoglobulin E; D000094632:Thymic Stromal Lymphopoietin
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