title: Na-glutamine co-transporters B(0)AT1 in villus and SN2 in crypts are differentially altered in chronically inflamed rabbit intestine.
abstract: Glutamine is a major nutrient utilized by the intestinal epithelium and is primarily assimilated via Na-glutamine co-transport (NGcT) on the brush border membrane (BBM) of enterocytes. Recently we reported that B(0)AT1 (SLC6A19) mediates glutamine absorption in villus while SN2 (SLC38A5) does the same in crypt cells. However, how B(0)AT1 and SN2 are affected during intestinal inflammation is unknown. In the present study it was shown that during chronic enteritis NGcT was inhibited in villus cells, however, it was stimulated in crypt cells. Our studies also demonstrated that the mechanism of inhibition of NGcT during chronic enteritis was secondary to a reduction in the number of B(0)AT1 co-transporters in the villus cell BBM without a change in the affinity of the co-transporter. In contrast, stimulation of NGcT in crypt cells was secondary to an increase in the affinity of SN2 for glutamine without an alteration in the number of co-transporters. Thus, glutamine assimilation which occurs via distinct transporters in crypt and villus cells is altered in the chronically inflamed intestine.
authors.affiliation: West Virginia University Health Science Centre, Morgantown, WV, USA.
authors.forename: Prosenjit
authors.initials: P
authors.lastname: Saha
authors.forename: Subha
authors.initials: S
authors.lastname: Arthur
authors.forename: Ramesh
authors.initials: R
authors.lastname: Kekuda
authors.forename: Uma
authors.initials: U
authors.lastname: Sundaram
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002908:Chronic Disease; D004751:Enteritis / Q000378:metabolism* / Q000473:pathology; D005973:Glutamine / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011817:Rabbits
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D005973:Glutamine