title: Differential function of lysophosphatidic acid receptors in cell proliferation and migration of neuroblastoma cells.
abstract: Lysophosphatidic acid (LPA) is a bioactive lipid mediator that induces diverse cellular biological effects and interacts with G protein-coupled transmembrane LPA receptors. In the present study, to assess biological roles of LPA receptors in the pathogenesis of tumor cells, each LPA receptor (Lpar1, Lpar2 or Lpar3)-expressing rat neuroblastoma B103 cells (lpa1-1, lpa2-2 or lpa3-3-2 cells, respectively) were used. In cell motility and invasion assay, lpa2-2 and lpa3-3-2 cells showed significant higher intrinsic activity without LPA treatment than LPA receptor-unexpressing AB2-1bf cells. LPA treatment further increased cell motility of these cells, which was suppressed by the pretreatment with inhibitors of Gi, Gq protein, or ROCK. By contrast, lpa1-1 cells markedly decreased intrinsic cell motility and invasion, compared with AB2-1bf cells. Constitutively active mutant Lpar1-expressing cells (lpa1Δ-1) showed significant high motility, comparable with those of lpa2-2 and lpa3-3-2. In soft agar assay, lpa3-3-2 and lpa1Δ-1 cells showed colony formation, but other cells failed. These results suggest that LPA receptors may play different roles in cell proliferation and migration of rat neuroblastoma cells.
authors.affiliation: Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Hayashi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Okabe
authors.forename: Kohei
authors.initials: K
authors.lastname: Kato
authors.forename: Mai
authors.initials: M
authors.lastname: Okumura
authors.forename: Rie
authors.initials: R
authors.lastname: Fukui
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fukushima
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D009361:Neoplasm Invasiveness; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D049368:Receptors, Lysophosphatidic Acid