title: [Histone H3 acetylation of tumor necrosis factor-alpha and cyclooxygenase-2 in patients with type 2 diabetes].
abstract: OBJECTIVE
  To investigate the expression of tumor necrosis factor-alpha (TNF-α) and cyclooxygenase-2 (COX-2) mRNA and evaluate the status of histone H3 acetylation at TNF-α and COX-2 promoter in peripheral blood mononuclear cells (PBMCs) from type 2 diabetics.
  
  
  METHODS
  The PBMCs from 12 type 2 diabetics and 12 healthy controls were isolated by Ficoll-Hypaque density gradient centrifugation. The differential expression of TNF-α and COX-2 mRNA was measured by real-time PCR (polymerase chain reaction). Chromatin immunoprecipitation analysis was used to detect the status of H3 acetylation at TNF-α and COX-2 promoter region.
  
  
  RESULTS
  TNF-α and COX-2 mRNA were overexpressed in PBMCs from Type 2 diabetics as compared with normal controls (2.28 ± 0.09 fold and 2.78 ± 0.26 fold).(P < 0.05). Compared with normal controls, H3 acetylation at the TNF-α (1.54 ± 0.43 vs 0.97 ± 0.39, P = 0.0094) and COX-2 (1.20 ± 0.58 vs 0.64 ± 0.21, P = 0.0161) gene promoter region was elevated in PBMCs from Type 2 diabetic patients.
  
  
  CONCLUSIONS
  Increased H3 acetylation at TNF-α and COX-2 promoter in PBMCs from type 2 diabetics may contribute to the pathogenesis of type 2 diabetes through the elevated expressions of TNF-α and COX-2.
authors.affiliation: Department of Endocrinology, Central South University, Changsha, Hunan, China.
authors.forename: Can
authors.initials: C
authors.lastname: Hou
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Yi-jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Qian-jin
authors.initials: QJ
authors.lastname: Lu
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Zhou
mesh_terms: D000107:Acetylation; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D006657:Histones; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human