title: Isolation and characterization of a novel plasma membrane intrinsic protein gene, LcPIP1, in Leymus chinensis that enhances salt stress tolerance in Saccharomyces cerevisiae.
abstract: A novel plasma membrane intrinsic, LcPIP1, was isolated from Leymus chinensis using RACE method. The LcPIP1 has 288 amino acids with an estimated molecular mass of 30.6 kDa. Semi RT-PCR analysis indicated that the expression level of LcPIP1 was obviously higher in leaf than root. The LcPIP1 was also found to be induced by salt stress. In addition, transformed with the LcPIP1, Saccharomyces cerevisiae could increase tolerance to salt stress. These results indicate that the LcPIP1 gene seems to play a role in resistance against salt stress.
authors.affiliation: College of Life Sciences, Northwest A & F University, Yangling 712100, People's Republic of China.
authors.forename: Pengda
authors.initials: P
authors.lastname: Ma
authors.forename: Jingying
authors.initials: J
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D006109:Poaceae / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride