title: Peptide derived from Pvfp-1 as bioadhesive on bio-inert surface.
abstract: Surface property is one important characteristic of materials, especially for ones that are bio-inert but designed for bio-medical application. In this study, we designed a series of peptides and compared their capacities as bioadhesive to improve the surface bioactivity of bio-inert material. The peptides were designed according to the sequence of Perna viridis foot protein 1 (Pvfp-1), one of the Mfp-1s (mussel foot protein 1) which play key roles in wet adhesion of mussel byssus. And the Teflon (PTFE) was chosen as a model of bio-inert material. With adsorption, adhesion and coating analysis, it was found that peptide C2 (M) (derived from the non-repeating region of Pvfp-1, contains modified DOPA) has superior coating and adhesion abilities especially on the bio-inert surface of PTFE. After coating with peptide C2 (M), the cell adhesion and spreading of osteoblast MC3T3-E1 cells on PTFE were significantly improved compared with those on non-coated surface, and the peptide-coating did not show any cell toxicity. Therefore, peptide C2 (M) is effective for improving the bioactivity of bio-inert PTFE, and could be potentially used as a bioadhesive on other bio-inert materials for biomedical application. Moreover, this study also provided new insights in designing other peptide-based bioadhesive materials.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yabiao
authors.initials: Y
authors.lastname: Yu
authors.forename: Lina
authors.initials: L
authors.lastname: Du
authors.forename: Xiyu
authors.initials: X
authors.lastname: Ding
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D004295:Dihydroxyphenylalanine / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D049883:Perna / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013499:Surface Properties; D014014:Tissue Adhesives / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D020099:Coated Materials, Biocompatible; D010455:Peptides; D014014:Tissue Adhesives; D004295:Dihydroxyphenylalanine; D011138:Polytetrafluoroethylene