title: Bioactive actions of pomegranate fruit extracts on leukemia cell lines in vitro hold promise for new therapeutic agents for leukemia.
abstract: Studies suggest that pomegranates contain bioactive chemicals with potential for treatment and prevention of cancer. Pomegranate juice extracts (PJE) have been shown to inhibit cellular proliferation and tumor growth and induce cell death via apoptosis in a number of cancer cell lines. However, to date, few studies have investigated the potential of PJE in the treatment of leukemia. We investigated the potential effect of PJE on induction of apoptosis and inhibition of cellular proliferation in 8 leukemia cell lines (4 lymphoid and 4 myeloid) and nontumor hematopoietic stem cells (control cells). Apoptosis was assessed by 2 methods: Annexin V-FITC/propidium iodide staining with flow cytometric analysis and 4'-6-diamidino-2-phenylindole (DAPI) morphological assessment. Cell cycle stage was investigated using propidum iodide staining of DNA content and flow cytometric analysis. Live cell counts were also performed using a trypan exclusion assay. PJE significantly induced apoptosis in all cell lines, including nontumor control cells, although lymphoid cells and 2 of the myeloid cell lines were more sensitive. Furthermore, PJE induced cell cycle arrest. These results were confirmed by DAPI analysis and viable cell counts using trypan blue exclusion assay. Our results provide evidence that PJE contain bioactive compounds that could be used in the treatment of leukemia.
authors.affiliation: Biomedical Research Centre, Faculty of Health and Well Being, Sheffield Hallam University, Sheffield, UK.
authors.forename: Haytham
authors.initials: H
authors.lastname: Dahlawi
authors.forename: Nicola
authors.initials: N
authors.lastname: Jordan-Mahy
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Clench
authors.forename: Christine L
authors.initials: CL
authors.lastname: Le Maitre
mesh_terms: D017302:Annexins / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000032:analysis; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D029561:Lythraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D017302:Annexins; D000972:Antineoplastic Agents, Phytogenic; D005456:Fluorescent Dyes; D007211:Indoles; D010936:Plant Extracts; C007293:DAPI