title: [Association between polymorphisms of metabolic genes and telomere length in workers exposed to polycyclic aromatic hydrocarbon].
abstract: OBJECTIVE
  To investigate the association between the polymorphisms of metabolic genes and telomere length of genomic DNA in peripheral blood of workers exposed to polycyclic aromatic hydrocarbons (PAHs).
  
  
  METHODS
  One hundred and forty five coke-oven workers exposed to PAHs and sixty eight non-exposed medical staffs were recruited in this study. Urinary 1-hydroxypyrene (1-OHP) served as the internal exposure dose of PAHs for all subjects. Relative telomere length (RTL) of genomic DNA in peripheral blood was used as telomere length and measured by real-time PCR. Polymorphisms of metabolic genes were detected by PCR-based methods.
  
  
  RESULTS
  Compared with control group, the exposure group shown a decreased RTL (1.10 +/- 0.75 vs 1.43 +/- 1.06, P < 0.05). In the coke-oven workers, after adjusting the sex, age, cigarettes per day and urinary 1-OHP, RTL (1.25 +/- 0.93) of workers with CT genotype at the CYP1A1 3801 T > C was significantly longer than that (0.93 +/- 0.51) of workers with TT genotype (P < 0.05). RTL (0.90 +/- 0.58) of individuals with the Tyr/His genotype at mEH Tyr113His was significantly shorter than that (1.24 +/- 0.90) of individuals with the Tyr/Tyr genotype (P < 0.05). RTL (1.02 +/- 0.64) of individuals with the CT genotype at AHR rs10250822 was significantly shorter than that (1.36 +/- 1.14) of individuals with the CC genotype (P < 0.05). RTL (0.93 +/- 0.54) of individuals with the AT genotype at AHR rs10247158 was significantly shorter than that (1.19 +/- 0.84) of individuals with the AA genotype (P < 0.05).
  
  
  CONCLUSIONS
  The results of present study suggested that PAHs exposure could induce the shorted RTL, CYP1A1, mEH, AHR polymorphisms might influence the change of telomere length of genomic DNA in peripheral blood of workers exposed to PAHs.
authors.affiliation: National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Bin
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Leng
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Duan
authors.forename: Zu-Fei
authors.initials: ZF
authors.lastname: Pan
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Dai
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zheng
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D020641:Polymorphism, Single Nucleotide; D016615:Telomere / Q000235:genetics*; D055815:Young Adult
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D004247:DNA; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1