title: An optimum fermentation model established by genetic algorithm for biotransformation from crude polydatin to resveratrol.
abstract: Natural resveratrol is widely used in medicine, food, and cosmetic because of its pharmacological properties. Due to its low content in plants, this study was conducted to increase the yield of resveratrol by microorganism transformation. Fungi Aspergillus niger AN-2436 was employed in biotransformation to produce resveratrol from polydatin, and genetic algorithm (GA) was used to optimize the fermentation conditions. A transformation ratio of higher than 95% was achieved in the following conditions: culture temperature of 30.3 °C, inoculum size of 20% (v/v), rotating speed of 147 rpm, and cultivation time of 36 h. Compared with the polydatin absorbance under the experimental conditions obtained by single-factor, orthogonal experiments and average absorbance, the GA provides the optimum experimental conditions, under which the largest transformation rate was achieved. The final transformation product obtained was identified as resveratrol, and it was proved by high-performance liquid chromatography, infrared, mass spectrometry, and nuclear magnetic resonance.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chong
authors.forename: Aixia
authors.initials: A
authors.lastname: Yan
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yang
authors.forename: Yangliu
authors.initials: Y
authors.lastname: Cai
authors.forename: Jinchun
authors.initials: J
authors.lastname: Chen
mesh_terms: D000465:Algorithms; D001234:Aspergillus niger / Q000378:metabolism*; D001711:Biotransformation; D005285:Fermentation; D005960:Glucosides / Q000378:metabolism*; D008954:Models, Biological; D017711:Nonlinear Dynamics; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*
chemical_list: D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin