title: Transformation of Solanum tuberosum plastids allows high expression levels of β-glucuronidase both in leaves and microtubers developed in vitro.
abstract: Plastid genome transformation offers an attractive methodology for transgene expression in plants, but for potato, only expression of gfp transgene (besides the selective gene aadA) has been published. We report here successful expression of β-glucuronidase in transplastomic Solanum tuberosum (var. Desiree) plants, with accumulation levels for the recombinant protein of up to 41% of total soluble protein in mature leaves. To our knowledge, this is the highest expression level reported for a heterologous protein in S. tuberosum. Accumulation of the recombinant protein in soil-grown minitubers was very low, as described in previous reports. Interestingly, microtubers developed in vitro showed higher accumulation of β-glucuronidase. As light exposure during their development could be the trigger for this high accumulation, we analyzed the effect of light on β-glucuronidase accumulation in transplastomic tubers. Exposure to light for 8 days increased β-glucuronidase accumulation in soil-grown tubers, acting as a light-inducible expression system for recombinant protein accumulation in tuber plastids. In this paper we show that plastid transformation in potato allows the highest recombinant protein accumulation in foliar tissue described so far for this food crop. We also demonstrate that in tubers high accumulation is possible and depends on light exposure. Because tubers have many advantages as protein storage organs, these results could lead to new recombinant protein production schemes based on potato.
authors.affiliation: Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular-INGEBI-CONICET, Vuelta de Obligado 2490, 2do. Piso, C1428ADN, Ciudad Autónoma de Buenos Aires, Argentina. segretin@dna.uba.ar
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Segretin
authors.forename: Ezequiel Matías
authors.initials: EM
authors.lastname: Lentz
authors.forename: Sonia Alejandra
authors.initials: SA
authors.lastname: Wirth
authors.forename: Mauro Miguel
authors.initials: MM
authors.lastname: Morgenfeld
authors.forename: Fernando Félix
authors.initials: FF
authors.lastname: Bravo-Almonacid
mesh_terms: D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D035281:Plant Tubers / Q000201:enzymology / Q000235:genetics; D030821:Plants, Genetically Modified; D018087:Plastids / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D005966:Glucuronidase
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