title: Direct transcriptional control of a p38 MAPK pathway by the circadian clock in Neurospora crassa.
abstract: MAPK signal transduction pathways are important regulators of stress responses, cellular growth, and differentiation. In Neurospora, the circadian clock controls rhythms in phosphorylation of the p38-like MAPK (OS-2); however, the mechanism for this regulation is not known. We show that the WCC, a transcription factor and clock component, binds to the os-4 MAPKKK promoter in response to light and rhythmically in constant darkness, peaking in the subjective morning. Deletion of the WCC binding sites in the os-4 promoter disrupts both os-4 mRNA and OS-2 phosphorylation rhythms. The clock also indirectly regulates rhythmic expression of the histidyl-phosphotransferase gene, hpt-1, which peaks in the evening. Anti-phase expression of positive (OS-4) and negative (HPT-1) MAPK pathway regulators likely coordinate to enhance rhythmic MAPK activation to prepare cells to respond to osmotic stress during the day in the natural environment. Consistent with this idea, we show that wild type cells have a clock-dependent morning kinetic advantage in glycerol accumulation after salt stress as compared to evening treatment. Thus, circadian transcriptional control of MAPK pathway components leads to striking time-of-day-specific effects on the signaling status and physiological response of the pathway.
authors.affiliation: Program for the Biology of Filamentous Fungi, Interdisciplinary Program in Genetics, Department of Biology, Center for Biological Clocks Research, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Lamb
authors.forename: Charles S
authors.initials: CS
authors.lastname: Goldsmith
authors.forename: Lindsay
authors.initials: L
authors.lastname: Bennett
authors.forename: Katelyn E
authors.initials: KE
authors.lastname: Finch
authors.forename: Deborah
authors.initials: D
authors.lastname: Bell-Pedersen
mesh_terms: D057906:Circadian Clocks / Q000235:genetics; D002940:Circadian Rhythm; D005656:Fungal Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009492:Neurospora crassa / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D014158:Transcription, Genetic; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020928:Mitogen-Activated Protein Kinases; C450461:os-2 protein, Neurospora crassa; D048051:p38 Mitogen-Activated Protein Kinases
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