title: Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia.
abstract: Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia.
authors.affiliation: Microbiological Diseases and Diagnostics Research, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, 2568, Australia.
authors.forename: Sam
authors.initials: S
authors.lastname: Abraham
authors.forename: James
authors.initials: J
authors.lastname: Chin
authors.forename: Huub J M
authors.initials: HJ
authors.lastname: Brouwers
authors.forename: Ren
authors.initials: R
authors.lastname: Zhang
authors.forename: Toni A
authors.initials: TA
authors.lastname: Chapman
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001315:Australia; D015373:Bacterial Typing Techniques / Q000379:methods*; D054307:Enterotoxigenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology*