title: Catecholamine analysis with strong cation exchange column liquid chromatography-peroxyoxalate chemiluminescence reaction detection.
abstract: A liquid chromatography-chemiluminescence detection method was developed and validated for the determination of catecholamines (norepinephrine, epinephrine, and dopamine) in mouse brains. Chromatography was performed on a strong cation exchange column (150 × 2.0-mm id) using an isocratic mobile phase of 65 mM potassium acetate/75 mM potassium phosphate (95:5, pH 3.5) at a flow rate of 0.2 mL/min following post-column fluorescence derivatization of catecholamines with ethylenediamine and peroxyoxalate chemiluminescence reaction detection. The recovery of catecholamines added to mouse brain samples was more than 95.0%, while intra- and inter-day precision of the assay were <4.8%. The validated method was used to determine norepinephrine and dopamine concentrations in mouse brains without prior sample purification.
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. makotot@mol.f.u-tokyo.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004298:Dopamine / Q000032:analysis; D004837:Epinephrine / Q000032:analysis; D004867:Equipment Design; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000032:analysis; D010070:Oxalates / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D002395:Catecholamines; D002412:Cations; D010070:Oxalates; C044622:peroxyoxalate; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine