title: Cytokinesis remnants define first neuronal asymmetry in vivo.
abstract: Polarization of a neuron begins with the appearance of the first neurite, thus defining the ultimate growth axis. Unlike late occurring polarity events (such as axonal growth), very little is known about this fundamental process. We show here that, in Drosophila melanogaster neurons in vivo, the first membrane deformation occurred 3.6 min after precursor division. Clustering of adhesion complex components (Bazooka (Par-3), cadherin-catenin) marked this place by 2.8 min after division; the upstream phosphatidylinositol 4,5-bisphosphate, by 0.7 min after division; and the furrow components RhoA and Aurora kinase, from the time of cytokinesis. Local DE-cadherin inactivation prevented sprout formation, whereas perturbation of division orientation did not alter polarization from the cytokinesis pole. This is, to our knowledge, the first molecular study of initial neuronal polarization in vivo. The mechanisms of polarization seem to be defined at the precursor stage.
authors.affiliation: Vlaams Instituut voor Biotechnologie, Department of Molecular and Developmental Genetics, Campus Gasthuisberg, Leuven, Belgium.
authors.forename: Giulia
authors.initials: G
authors.lastname: Pollarolo
authors.forename: Joachim G
authors.initials: JG
authors.lastname: Schulz
authors.forename: Sebastian
authors.initials: S
authors.lastname: Munck
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Dotti
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D002502:Centrioles / Q000502:physiology; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D006735:Horseradish Peroxidase / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D008164:Luminescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D009154:Mutation / Q000235:genetics; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D011679:Pupa; D012679:Sense Organs / Q000166:cytology / Q000196:embryology; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D051178:alpha Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D008164:Luminescent Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D051178:alpha Catenin; C081439:alpha-Cat protein, Drosophila; D006735:Horseradish Peroxidase
references.citation: Nat Immunol. 2006 Nov;7(11):1143-9
references.pmid: 17053799
references.citation: Curr Biol. 2007 Oct 23;17(20):1735-45
references.pmid: 17935995
references.citation: Annu Rev Neurosci. 2009;32:347-81
references.pmid: 19400726
references.citation: J Cell Biol. 2007 Jul 30;178(3):517-27
references.pmid: 17646397
references.citation: Nature. 1998 May 14;393(6681):178-81
references.pmid: 9603522
references.citation: Development. 1989 Oct;107(2):389-405
references.pmid: 2517255
references.citation: Development. 1996 Jun;122(6):1673-82
references.pmid: 8674407
references.citation: J Biol Chem. 2005 Nov 11;280(45):37901-7
references.pmid: 16162509
references.citation: Nat Cell Biol. 2010 Jun;12(6):533-42
references.pmid: 20453849
references.citation: Nat Cell Biol. 2009 Sep;11(9):1057-68
references.pmid: 19668197
references.citation: J Cell Sci. 2008 Jan 15;121(Pt 2):178-85
references.pmid: 18187450
references.citation: Proc Natl Acad Sci U S A. 2009 May 19;106(20):8284-9
references.pmid: 19429710
references.citation: Curr Biol. 2007 May 15;17(10):834-43
references.pmid: 17475495
references.citation: Curr Biol. 2002 Apr 16;12(8):640-7
references.pmid: 11967150
references.citation: Dev Biol. 1994 Oct;165(2):716-26
references.pmid: 7958432
references.citation: Mol Biol Cell. 2005 Sep;16(9):4061-72
references.pmid: 15975913
references.citation: Development. 2008 Dec;135(23):3829-38
references.pmid: 18948416
references.citation: Mol Biol Cell. 2009 May;20(10):2540-8
references.pmid: 19297522
references.citation: Nature. 2005 Aug 4;436(7051):704-8
references.pmid: 16079847
references.citation: Nat Cell Biol. 2002 Jun;4(6):408-15
references.pmid: 11992112
references.citation: PLoS Biol. 2009 Nov;7(11):e1000240
references.pmid: 19901980
references.citation: Nat Rev Mol Cell Biol. 2003 Nov;4(11):842-54
references.pmid: 14625535
references.citation: PLoS One. 2011;6(9):e24190
references.pmid: 21935383
references.citation: Cell. 2010 May 28;141(5):757-74
references.pmid: 20510924
references.citation: Nat Rev Mol Cell Biol. 2007 Aug;8(8):633-44
references.pmid: 17643125
references.citation: Cell. 1994 Nov 4;79(3):507-13
references.pmid: 7954816
references.citation: Cold Spring Harb Perspect Biol. 2009 Sep;1(3):a001644
references.pmid: 20066106
references.citation: Trends Cell Biol. 2005 Dec;15(12):651-8
references.pmid: 16243528
references.citation: Cell. 2005 Sep 9;122(5):763-73
references.pmid: 16137758
references.citation: Neural Dev. 2007 Apr 30;2:7
references.pmid: 17470283
references.citation: Development. 1999 Aug;126(16):3573-84
references.pmid: 10409503
references.citation: Nat Cell Biol. 2010 Jan;12(1):60-9
references.pmid: 19966783
references.citation: Science. 2003 Dec 5;302(5651):1704-9
references.pmid: 14657486
references.citation: J Cell Biol. 2008 Apr 21;181(2):195-202
references.pmid: 18411311
references.citation: Trends Cell Biol. 2006 Jun;16(6):276-84
references.pmid: 16616849
references.citation: Curr Biol. 2005 Aug 9;15(15):1407-12
references.pmid: 16085494
references.citation: Nat Neurosci. 2004 Feb;7(2):136-44
references.pmid: 14703572
references.citation: Mol Cell. 2007 Dec 14;28(5):886-98
references.pmid: 18082612
references.citation: Microsc Res Tech. 1997 Dec 15;39(6):506-31
references.pmid: 9438251
references.citation: Cell. 2006 Jun 30;125(7):1375-86
references.pmid: 16814722
references.citation: Dev Biol. 1999 Dec 1;216(1):406-22
references.pmid: 10588889
references.citation: Cell. 1998 Oct 16;95(2):225-35
references.pmid: 9790529
references.citation: Mech Dev. 2008 Mar-Apr;125(3-4):325-36
references.pmid: 18083504
references.citation: Curr Biol. 1998 Mar 12;8(6):343-6
references.pmid: 9512420
references.citation: Curr Biol. 2010 Apr 13;20(7):636-42
references.pmid: 20303268
references.citation: Curr Biol. 2005 Aug 9;15(15):1401-6
references.pmid: 16085493
references.citation: J Biol Chem. 2009 Nov 27;284(48):33571-9
references.pmid: 19812038
references.citation: Nature. 2003 Apr 17;422(6933):766-74
references.pmid: 12700771
references.citation: J Cell Biol. 2009 Jun 1;185(5):787-96
references.pmid: 19468069
references.citation: J Cell Biol. 2009 Jun 1;185(5):779-86
references.pmid: 19487453