title: Involvement of gonadotropins in the induction of hypertrophy-hyperplasia in the interstitial tissues of ovaries in neonatally diethylstilbestrol-treated mice.
abstract: Neonatally diethylstilbestrol (DES) treatment causes hypertrophy-hyperplasia in the interstitial tissue of mouse ovaries. To understand the induction mechanism of the hypertrophy, mRNA expression involved in steroidogenesis in the ovary of neonatally DES-treated mice was examined. The expression of StAR and Cyp11a1 was significantly reduced while Cyp19 and Sf-1 were stimulated in the ovary of neonatally DES-treated 3-month-old mice. Expression of those genes was not different between DES- and oil-treated mice after the gonadotropins treatment. Lhb in the pituitary of 3-month-old neonatally DES-treated mice was significantly decreased. Finally, ovaries from DES-treated mice transplanted to neonatally oil-treated hosts had developing follicles at several stages and corpora lutea, whereas grafted ovaries from neonatally oil-treated mice in 3-month-old neonatally DES-treated hosts showed lipid accumulation in the interstitial tissue. Thus, hypertrophy and accumulation of lipid droplets in interstitial cells of neonatally DES-treated mice is caused by impaired steroidogenesis due to the alterations of gonadotropins levels.
authors.affiliation: International Graduate School of Arts and Sciences, Yokohama City University, Yokohama 236-0027, Japan.
authors.forename: Hanako
authors.initials: H
authors.lastname: Kakuta
authors.forename: Masami
authors.initials: M
authors.lastname: Tanaka
authors.forename: Pierre
authors.initials: P
authors.lastname: Chambon
authors.forename: Hajime
authors.initials: H
authors.lastname: Watanabe
authors.forename: Taisen
authors.initials: T
authors.lastname: Iguchi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Sato
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001141:Aromatase / Q000235:genetics; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004054:Diethylstilbestrol / Q000008:administration & dosage / Q000633:toxicity*; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000172:deficiency / Q000187:drug effects / Q000235:genetics; D047629:Estrogen Receptor beta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D037201:Follicle Stimulating Hormone, beta Subunit / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D015292:Glycoprotein Hormones, alpha Subunit / Q000378:metabolism; D006062:Gonadotropins / Q000235:genetics / Q000378:metabolism*; D006965:Hyperplasia; D006984:Hypertrophy; D050356:Lipid Metabolism / Q000187:drug effects; D037101:Luteinizing Hormone, beta Subunit / Q000378:metabolism; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010052:Ovariectomy; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000637:transplantation; D010750:Phosphoproteins / Q000235:genetics; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D054339:Steroidogenic Factor 1 / Q000235:genetics; D013799:Theca Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004968:Estrogens, Non-Steroidal; D037201:Follicle Stimulating Hormone, beta Subunit; D015292:Glycoprotein Hormones, alpha Subunit; D006062:Gonadotropins; D037101:Luteinizing Hormone, beta Subunit; D010750:Phosphoproteins; D012333:RNA, Messenger; D054339:Steroidogenic Factor 1; D000098682:Steroidogenic Acute Regulatory Protein; D004958:Estradiol; D004054:Diethylstilbestrol; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1