title: Functionalized arrays of Raman-enhancing nanoparticles for capture and culture-free analysis of bacteria in human blood.
abstract: Detecting bacteria in clinical samples without using time-consuming culture processes would allow rapid diagnoses. Such a culture-free detection method requires the capture and analysis of bacteria from a body fluid, which are usually of complicated composition. Here we show that coating Ag-nanoparticle arrays with vancomycin (Van) can provide label-free analysis of bacteria via surface-enhanced Raman spectroscopy (SERS), leading to a ~1,000-fold increase in bacteria capture, without introducing significant spectral interference. Bacteria from human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, a Van-coated substrate provides distinctly different SERS spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance tests. Our results represent a critical step towards the creation of SERS-based multifunctional biochips for rapid culture- and label-free detection and drug-resistant testing of microorganisms in clinical samples.
authors.affiliation: Institute of Atomic and Molecular Sciences, Taipei, Taiwan.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Liu
authors.forename: Kun-Tong
authors.initials: KT
authors.lastname: Tsai
authors.forename: Huai-Hsien
authors.initials: HH
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Chen
authors.forename: Yuan-Chun
authors.initials: YC
authors.lastname: Chao
authors.forename: Hsuan-Hao
authors.initials: HH
authors.lastname: Chang
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Juen-Kai
authors.initials: JK
authors.lastname: Wang
authors.forename: Yuh-Lin
authors.initials: YL
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000187:drug effects*; D001769:Blood / Q000382:microbiology*; D016983:Enterococcus / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014640:Vancomycin / Q000737:chemistry
chemical_list: D012834:Silver; D014640:Vancomycin
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