title: (-)-Epigallocatechin-3-gallate: a novel storage medium for avulsed teeth.
abstract: The purpose of the present study was to evaluate the efficacy of (-)-epigallocatechin-3-gallate (EGCG) in maintaining the vitality of human periodontal ligament (PDL) cells when used as a storage medium for avulsed teeth prior to replantation. Thirty freshly extracted single-rooted human teeth with closed apices were randomly assigned to three experimental groups with 10 samples per group and immersed in one of the storage media: EGCG, Hank's balanced salt solution (HBSS), or milk for 2 h. The PDL cells were dissociated by an enzyme treatment with collagenase and trypsin. The cells were then labeled with 0.4% Trypan blue for the determination of viability. The result showed that EGCG group had the highest percentage of cell viability, followed by HBSS and milk group, in descending order.
authors.affiliation: Department of Stomatology, Xianning University, Xianning, China. chenhuangqin79@163.com
authors.forename: Huangqin
authors.initials: H
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D008892:Milk; D019188:Organ Preservation Solutions / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology*; D014084:Tooth Avulsion / Q000628:therapy*
chemical_list: C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D002392:Catechin; C045651:epigallocatechin gallate