title: Adipokinetic hormone-induced antioxidant response in Spodoptera littoralis.
abstract: The antioxidative potential of the Manduca sexta adipokinetic hormone (Manse-AKH) in the last instar larvae of Spodoptera littoralis (Noctuidae, Lepidoptera) was demonstrated after exposure to oxidative stress (OS) elicited by feeding on artificial diet containing tannic acid (TA). Determination of protein carbonyls (PCs) and reduced glutathione (GSH) levels, monitoring of activity of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferases (GSTs), as well as measuring of the mRNA expression of CAT and SOD were used as markers of the OS. Injection of the Manse-AKH (5 pmol per individual) reversed the OS status by mitigation of PCs formation and by stimulation of glutathione-S-transferases (GSTs) activity. The CAT and SOD mRNA expression was significantly suppressed after the Manse-AKH injection while activity of these enzymes was not affected. These results indicate that diminishing of OS after the AKH injection might be a result of activation of specific enzymatic pathway possibly at the post-translational level rather than a direct effect on regulation of antioxidant marker genes at the transcriptional level.
authors.affiliation: Institute of Entomology, Biology Centre, Academy of Sciences, Branišovská 31, České Budějovice 370 05, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Večeřa
authors.forename: Natraj
authors.initials: N
authors.lastname: Krishnan
authors.forename: Axel
authors.initials: A
authors.lastname: Mithöfer
authors.forename: Heiko
authors.initials: H
authors.lastname: Vogel
authors.forename: Dalibor
authors.initials: D
authors.lastname: Kodrík
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004032:Diet; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007301:Insect Hormones / Q000494:pharmacology*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018402:Manduca / Q000737:chemistry; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013634:Tannins / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D007301:Insect Hormones; D019476:Insect Proteins; D009842:Oligopeptides; D013634:Tannins; C044833:adipokinetic hormone; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid