title: Enhanced uridine 5'-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux.
abstract: A whole-cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH(2)PO(4), MgCl(2) and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH(2)PO(4) 22.1 g/L; MgCl(2) 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: An
authors.initials: A
authors.lastname: Li
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xie
authors.forename: Jian
authors.initials: J
authors.lastname: Xiong
authors.forename: Jianxin
authors.initials: J
authors.lastname: Bai
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Chen
authors.forename: Huanqing
authors.initials: H
authors.lastname: Niu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000737:chemistry / Q000378:metabolism; D009963:Orotic Acid / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000096:biosynthesis*
chemical_list: D010710:Phosphates; D015636:Magnesium Chloride; D009963:Orotic Acid; D014542:Uridine Monophosphate; C018279:sodium phosphate