title: Zilpaterol hydrochloride alters abundance of β-adrenergic receptors in bovine muscle cells but has little effect on de novo fatty acid biosynthesis in bovine subcutaneous adipose tissue explants.
abstract: We predicted that zilpaterol hydrochloride (ZH), a β-adrenergic receptor (AR) agonist, would depress mRNA and protein abundance of β-AR in bovine satellite cells. We also predicted that ZH would decrease total lipid synthesis in bovine adipose tissue. Bovine satellite cells isolated from the semimembranosus muscle were plated on tissue culture plates coated with reduced growth factor matrigel or collagen. Real-time quantitative PCR was used to measure specific gene expression after 48 h of ZH exposure in proliferating satellite cells and fused myoblasts. There was no effect of ZH dose on [(3)H]thymidine incorporation into DNA in proliferating myoblasts. Zilpaterol hydrochloride at 1 µM decreased (P < 0.05) β1-AR mRNA, and 0.01 and 1 µM ZH decreased (P < 0.05) β2-AR and β3-AR mRNA in myoblasts. The expression of IGF-I mRNA tended to increase (P = 0.07) with 1 µM ZH. There was no effect (P > 0.10) of ZH on the β-AR or IGF-I gene expression in fused myotube cultures at 192 h or on fusion percentage. The β2-AR antagonist ICI-118, 551 at 0.1 µM attenuated (P < 0.05) the effect of 0.1 µM ZH to reduce expression of β1- and β2-AR mRNA. The combination of 0.01 µM ZH and 0.1 µM ICI-118, 551 caused an increase (P < 0.05) in β1-AR gene expression. There was no effect (P > 0.10) of ICI-118, 551 or ZH on β3-AR or IGF-I. Western blot analysis revealed that the protein content of β2-AR in ZH-treated myotube cultures decreased (P < 0.05) relative to control. Total lipid synthesis from acetate was increased by ZH in bovine subcutaneous adipose tissue explants in the absence of theophylline but was decreased by ZH when theophylline was included in the incubation medium. These data indicate that ZH alters mRNA and protein concentrations of β-AR in satellite cell cultures, which in turn could affect responsiveness of cells to prolonged ZH exposure in vivo. Similar to other β-adrenergic agonists, ZH had only modest effects on lipid metabolism in adipose tissue explants.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA.
authors.forename: E K
authors.initials: EK
authors.lastname: Miller
authors.forename: K Y
authors.initials: KY
authors.lastname: Chung
authors.forename: J P
authors.initials: JP
authors.lastname: Hutcheson
authors.forename: D A
authors.initials: DA
authors.lastname: Yates
authors.forename: S B
authors.initials: SB
authors.lastname: Smith
authors.forename: B J
authors.initials: BJ
authors.lastname: Johnson
mesh_terms: D058672:Adrenergic beta-2 Receptor Antagonists / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects*; D032446:Myoblasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000187:drug effects*; D032496:Satellite Cells, Skeletal Muscle / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D050151:Subcutaneous Fat / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D014297:Trimethylsilyl Compounds / Q000494:pharmacology*
chemical_list: D058672:Adrenergic beta-2 Receptor Antagonists; D005227:Fatty Acids; D011943:Receptors, Adrenergic, beta; D014297:Trimethylsilyl Compounds; C475840:Zilpaterol