title: An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy.
abstract: A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.
authors.affiliation: Graduate School of Engineering, Yokohama National University, Yokohama, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Tomonaga
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Hidaka
authors.forename: Izuru
authors.initials: I
authors.lastname: Kawamura
authors.forename: Takudo
authors.initials: T
authors.lastname: Nishio
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ohsawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Okitsu
authors.forename: Akimori
authors.initials: A
authors.lastname: Wada
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sudo
authors.forename: Naoki
authors.initials: N
authors.lastname: Kamo
authors.forename: Ayyalusamy
authors.initials: A
authors.lastname: Ramamoorthy
authors.forename: Akira
authors.initials: A
authors.lastname: Naito
mesh_terms: D025602:Halorhodopsins / Q000378:metabolism* / Q000528:radiation effects*; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D050505:Mutant Proteins / Q000378:metabolism / Q000528:radiation effects; D025603:Sensory Rhodopsins / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D025602:Halorhodopsins; D050505:Mutant Proteins; D025603:Sensory Rhodopsins; C061508:sensory rhodopsin II protein, archaeal
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