title: Biophysical characterization of glycosaminoglycan-IL-7 interactions using SPR.
abstract: Glycosaminoglycans (GAGs) interact with a number of cytokines and growth factors thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and the regulation of the tissue distribution of cytokines/growth factors. In the present study, we employed surface plasmon resonance (SPR) spectroscopy to dissect the binding interactions between GAGs and murine and human forms of interleukin-7 (IL-7). SPR results revealed that heparin binds with higher affinity to human IL-7 than murine IL-7 through a different kinetic mechanism. The optimal oligosaccharide length of heparin for the interactions to human and murine IL-7 involves a sequence larger than a tetrasaccharide. These results further demonstrate that while IL-7 is principally a heparin/heparan sulfate binding protein, it also interacts with dermatan sulfate, chondroitin sulfates C, D, and E, indicating that this cytokine preferentially interacts with GAGs having a higher degree of sulfation.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. zhangf2@rpi.edu
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Xinle
authors.initials: X
authors.lastname: Liang
authors.forename: Dennis
authors.initials: D
authors.lastname: Pu
authors.forename: Kasim I
authors.initials: KI
authors.lastname: George
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Holland
authors.forename: Scott T R
authors.initials: ST
authors.lastname: Walsh
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000818:Animals; D001703:Biophysics; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D015851:Interleukin-7 / Q000737:chemistry*; D051379:Mice; D020349:Surface Plasmon Resonance
chemical_list: D006025:Glycosaminoglycans; D015851:Interleukin-7
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