title: An efficient vector system to modify cells genetically.
abstract: The transfer of foreign genes into mammalian cells has been essential for understanding the functions of genes and mechanisms of genetic diseases, for the production of coding proteins and for gene therapy applications. Currently, the identification and selection of cells that have received transferred genetic material can be accomplished by methods, including drug selection, reporter enzyme detection and GFP imaging. These methods may confer antibiotic resistance, or be disruptive, or require special equipment. In this study, we labeled genetically modified cells with a cell surface biotinylation tag by co-transfecting cells with BirA, a biotin ligase. The modified cells can be quickly isolated for downstream applications using a simple streptavidin bead method. This system can also be used to screen cells expressing two sets of genes from separate vectors.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Huamin
authors.initials: H
authors.lastname: Han
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: He
authors.forename: Kristy
authors.initials: K
authors.lastname: Ong
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
mesh_terms: D019921:Biotinylation / Q000235:genetics / Q000502:physiology*; D019731:Carbon-Nitrogen Ligases / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000009:adverse effects / Q000235:genetics*; D006801:Humans; D014162:Transfection
chemical_list: D019731:Carbon-Nitrogen Ligases
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