title: Effects of the commercial extract of aronia on oxidative stress in blood platelets isolated from breast cancer patients after the surgery and various phases of the chemotherapy.
abstract: Since the extract from berries of Aronia melanocarpa presents antioxidative properties in plasma and in blood platelets, not only from healthy group, but also from patients with benign breast diseases and in patients with invasive breast cancer before surgery, the aim of our present study was to evaluate the oxidative stress by measuring the level of various biomarkers of this process such as the generation of superoxide anion radicals (O(2)(-·)), the amount of carbonyl groups and 3-nitrotyrosine in proteins or the amount of glutathione in blood platelets isolated from breast cancer patients after the surgery and after various phases of the chemotherapy in the presence of A. melanocarpa extract (Aronox) in vitro. We demonstrated in platelet proteins from patients with invasive breast cancer (after the surgery and after various phases of the chemotherapy) higher level of carbonyl groups than in control healthy group. The level of 3-nitrotyrosine in platelet proteins from patients with invasive breast cancer was also significantly higher than in healthy subject group. We observed an increase of other biomarkers of oxidative stress such as O(2)(-·) and a decrease of GSH in platelets from patients with breast cancer (after the surgery and after various phases of the chemotherapy) compared to the healthy group. In model system in vitro our results showed that the commercial extract from berries of A. melanocarpa due to antioxidant action, significantly reduced the oxidative/nitrative stress in platelets from patients with invasive breast cancer caused by the surgery and various phases of the chemotherapy.
authors.affiliation: Department of General Biochemistry, University of Lodz, Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kedzierska
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.forename: Barbara
authors.initials: B
authors.lastname: Wachowicz
authors.forename: Rafal
authors.initials: R
authors.lastname: Glowacki
authors.forename: Edward
authors.initials: E
authors.lastname: Bald
authors.forename: Urszula
authors.initials: U
authors.lastname: Czernek
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Szydłowska-Pazera
authors.forename: Piotr
authors.initials: P
authors.lastname: Potemski
authors.forename: Janusz
authors.initials: J
authors.lastname: Piekarski
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Jeziorski
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects*; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy*; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy*; D005260:Female; D005638:Fruit / Q000737:chemistry; D005978:Glutathione / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D046936:Photinia / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013481:Superoxides / Q000097:blood; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010636:Phenols; D010936:Plant Extracts; D013481:Superoxides; C002744:3-nitrotyrosine; D014443:Tyrosine; D005978:Glutathione