title: Phenotypic and genetic analyses of 111 clinical and environmental O1, O139, and non-O1/O139 Vibrio cholerae strains from different geographical areas.
abstract: A total of 111 clinical and environmental O1, O139 and non-O1/O139 Vibrio cholerae strains isolated between 1978 and 2008 from different geographical areas were typed using a combination of methods: antibiotic susceptibility, biochemical test, serogroup, serotype, biotype, sequences containing variable numbers of tandem repeats (VNTRs) and virulence genes ctxA and tcpA amplification. As a result of the performed typing work, the strains were organized into four clusters: cluster A1 included clinical O1 Ogawa and O139 serogroup strains (ctxA(+) and tcpA(+)); cluster A2 included clinical non-O1/O139 strains (ctxA(-) and tcpA(-)), as well as environmental O1 Inaba and non-O1/O139 strains (ctxA(-) and tcpA(-)/tcpA(+)); cluster B1 contained two clinical O1 strains and environmental non-O1/O139 strains (ctxA(-) and tcpA(+)/tcpA(-)); cluster B2 contained clinical O1 Inaba and Ogawa strains (ctxA(+) and tcpA(+)). The results of this work illustrate the advantage of combining several typing methods to discriminate between clinical and environmental V. cholerae strains.
authors.affiliation: NBC and Materials Area, Instituto Tecnológico La Marañosa, Dirección General de Armamento y Material, Secretaría de Estado de Defensa, Spanish Ministry of Defence, San Martín de la Vega, Madrid, Spain. rselcan@oc.mde.es
authors.forename: R E
authors.initials: RE
authors.lastname: Sellek
authors.forename: M
authors.initials: M
authors.lastname: Niemcewicz
authors.forename: J S
authors.initials: JS
authors.lastname: Olsen
authors.forename: O
authors.initials: O
authors.lastname: Bassy
authors.forename: P
authors.initials: P
authors.lastname: Lorenzo
authors.forename: L
authors.initials: L
authors.lastname: Martí
authors.forename: A
authors.initials: A
authors.lastname: Roszkowiak
authors.forename: J
authors.initials: J
authors.lastname: Kocik
authors.forename: J C
authors.initials: JC
authors.lastname: Cabria
mesh_terms: D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D018598:Minisatellite Repeats; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D037521:Virulence Factors
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D037521:Virulence Factors