title: [Study on of deferrization mechanism of apoferritin and the effect of spectra variation with holoferritin].
abstract: The deferrization mechanism of apoferritin was established, and the spectra variation of apoferritin was compared with that of holoferritin. Sodium hyposulfite is a strong reducing agent, therefore, was applied to deoxidize holoferritin to release iron ion, and connection of iron of buffer was measured by the 2,2-dipyridyl. Apoferritin was detected by ICP-MS. Holoferritin was found to have no absorption compared with apoferritin by UV analysis, and have no fluorescence emission spectra in contrast with apoferritin by fluorescence analysis.
authors.affiliation: College of Food, Shihezi University, Shihezi 832003, China. skyocean_2004@163.com
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun-Mao
authors.initials: JM
authors.lastname: Tong
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Zhao
mesh_terms: D015082:2,2'-Dipyridyl; D001052:Apoferritins / Q000737:chemistry*; D002021:Buffers; D005293:Ferritins; D007501:Iron; D013057:Spectrum Analysis
chemical_list: D002021:Buffers; D015082:2,2'-Dipyridyl; D005293:Ferritins; D001052:Apoferritins; D007501:Iron