title: Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy.
abstract: Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC-CM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSC-CMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the non-invasive purification of PSC-CMs.
authors.affiliation: NSF Center for Biophotonics Science and Technology, University of California, Davis, 2700 Stockton Blvd, Suite 1400, Sacramento, CA 95817, USA.
authors.forename: Samir
authors.initials: S
authors.lastname: Awasthi
authors.forename: Dennis L
authors.initials: DL
authors.lastname: Matthews
authors.forename: Ronald A
authors.initials: RA
authors.lastname: Li
authors.forename: Nipavan
authors.initials: N
authors.lastname: Chiamvimonvat
authors.forename: Deborah K
authors.initials: DK
authors.lastname: Lieu
authors.forename: James W
authors.initials: JW
authors.lastname: Chan
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D047569:Laser Scanning Cytometry / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D009218:Myosins
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