title: NADPH-diaphorase expression in the rat jejunum after intestinal ischemia/reperfusion.
abstract: The purpose of this study was to analyze the nicotinamide adenine dinucleotide phosphate - diaphorase (NADPH-d) activity in the rat jejunum after a mesenteric ischemia/reperfusion injury. Nitric oxide, synthetised from L-arginine by the enzyme nitric oxide synthase, is a nonadrenergic noncholinergic relaxant neurotransmitter of the intestinal smooth muscle. It plays an important role in the process of plasticity after the ischemia/reperfusion injury. Experimental animals were divided in two groups: the control group and the ischemic/reperfusion group, with different period of the reperfusion. The NADPH-d histochemical method has been used as a marker for the nitric oxide synthase. NADPH-d activity has been rapidly decreased in the neurons of both enteric nervous systems in plexuses of the jejunum after 1 h mesenteric ischemia and 1 h reperfusion. Differences were predominantly detected in the myenteric plexus; they were seen in change of the neuronal shape, in the arrangement of neurons and in intensity of their staining. The NADPH-d positivity was absent in the intestinal crypts. After 1 h ischemia and 24 h reperfusion, the NADPH-d activity was gradually increased, but it was lower in comparison with the control group. On the 30th day following the ischemia/reperfusion there were no changes in NADPH-d positivity compared with the control animals. These results indicated that the jejunal ischemia/reperfusion has affected the neurons of the enteric nervous system of adult rats and resulted in the early decrease of NADPH-d positivity 1 h of the reperfusion insult. The gradual increasing of NADPH-d activity in 24 h following the reperfusion could be considered as a result of the plasticity process. On the 30(th) day after the ischemia/reperfusion all histochemical changes were returned to the control levels.
authors.affiliation: Department of Anatomy, Pavol Jozef Safarik University, Kosice, Slokav Republic. a.bolek@seznam.cz
authors.forename: A
authors.initials: A
authors.lastname: Bolekova
authors.forename: T
authors.initials: T
authors.lastname: Spakovska
authors.forename: D
authors.initials: D
authors.lastname: Kluchova
authors.forename: S
authors.initials: S
authors.lastname: Toth
authors.forename: J
authors.initials: J
authors.lastname: Vesela
mesh_terms: D000818:Animals; D017615:Enteric Nervous System / Q000201:enzymology* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic; D007583:Jejunum / Q000294:innervation* / Q000473:pathology; D008297:Male; D009252:NADPH Dehydrogenase / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009474:Neurons / Q000201:enzymology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology* / Q000473:pathology; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins; D009252:NADPH Dehydrogenase
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