title: Phylogenetic microarray analysis of a microbial community performing reductive dechlorination at a TCE-contaminated site.
abstract: A high-density phylogenetic microarray (PhyloChip) was applied to track bacterial and archaeal populations through different phases of remediation at Ft. Lewis, WA, a trichloroethene (TCE)-contaminated groundwater site. Biostimulation with whey, and bioaugmentation with a Dehalococcoides-containing enrichment culture were strategies implemented to enhance dechlorination. As a measure of species richness, over 1300 operational taxonomic units (OTUs) were detected in DNA from groundwater samples extracted during different stages of treatment and in the bioaugmentation culture. In order to determine active members within the community, 16S rRNA from samples were analyzed by microarray and ∼600 OTUs identified. A cDNA clone library of the expressed 16S rRNA corroborated the observed diversity and activity of some of the phyla. Principle component analysis of the treatment plot samples revealed that the microbial populations were constantly changing during the course of the study. Dynamic analysis of the archaeal population showed significant increases in methanogens at the later stages of treatment that correlated with increases in methane concentrations of over 2 orders of magnitude. Overall, the PhyloChip analyses in this study have provided insights into the microbial ecology and population dynamics at the TCE-contaminated field site useful for understanding the in situ reductive dechlorination processes.
authors.affiliation: Department of Civil and Environmental Engineering, University of California-Berkeley, Berkeley, California, United States of America.
authors.forename: Patrick K H
authors.initials: PK
authors.lastname: Lee
authors.forename: F
authors.initials: F
authors.lastname: Warnecke
authors.forename: Eoin L
authors.initials: EL
authors.lastname: Brodie
authors.forename: Tamzen W
authors.initials: TW
authors.lastname: Macbeth
authors.forename: Mark E
authors.initials: ME
authors.lastname: Conrad
authors.forename: Gary L
authors.initials: GL
authors.lastname: Andersen
authors.forename: Lisa
authors.initials: L
authors.lastname: Alvarez-Cohen
mesh_terms: D001105:Archaea / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014241:Trichloroethylene / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene
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