title: The influence of peroxisome proliferator-activated receptor γ(1) during differentiation of mouse embryonic stem cells to neural cells.
abstract: Peroxisome proliferator activated receptor γ, belongs to PPARs, which exerts various metabolic functions including differentiation process. To testify the importance of PPARγ in neural differentiation of mouse embryonic stem cells (mESCs), its expression level was assessed. Data revealed an elevation in expression level of PPARγ when neural precursors (NPs) are formed upon retinoic acid treatment. Thus, involvement of PPARγ in two stages of neural differentiation of mESCs, during and post-NPs formation was examined by application of its agonist and antagonist. Our results indicated that PPARγ inactivation via treatment with GW9662 during NPs formation, reduced expression of neural precursor and neural (neuronal and astrocytes) markers. However, PPARγ inactivation by antagonist treatment post-NPs formation stage only decreased the expression of mature astrocyte marker (Gfap) suggesting that inactivation of PPARγ by antagonist decreased astrocyte differentiation. Here, we have demonstrated the stage dependent role of PPARγ modulation on neural differentiation of mESCs by retinoic acid treatment for the first time.
authors.affiliation: Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghoochani
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Shabani
authors.forename: Maryam
authors.initials: M
authors.lastname: Peymani
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Karamali
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Karbalaei
authors.forename: Somayeh
authors.initials: S
authors.lastname: Tanhaie
authors.forename: Ahmad
authors.initials: A
authors.lastname: Salamian
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Esmaeili
authors.forename: Sadegh
authors.initials: S
authors.lastname: Valian-Borujeni
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects / Q000235:genetics*; D009474:Neurons / Q000166:cytology; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D047495:PPAR gamma; D045162:Thiazolidinediones; C528900:glial fibrillary astrocytic protein, mouse; D000077154:Rosiglitazone; D014212:Tretinoin