title: EGRI and FOSB gene expressions in cancer stroma are independent prognostic indicators for epithelial ovarian cancer receiving standard therapy.
abstract: Stromal components interact with cancer cells to promote growth and metastasis. The purpose of this study was to identify genes expressed in stroma, which could provide prognostic information in epithelial ovarian cancer (EOC). Seventy-four patients were included. We performed gene expression profiling and confirmed array data using RT-PCR and immunohistochemistry. By microarray analysis, 52 candidate genes associated with progression free survival (PFS) were identified (P < 0.005). Expression of the early growth response 1 (EGR1) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) genes was further analyzed. Array data were confirmed by RT-PCR and multivariate analysis demonstrated that both EGR1 and FOSB expression in cancer stroma, and EGR1 expression in cancer are independent prognostic factors in EOC. Immunohistochemically, EGR1 protein is localized in cancer cells and α-smooth muscle actin positive stromal fibroblasts. The EGR1 and FOSB expression in stromal cells and EGR1 expression in cancer cells are prognostic indicators in EOC.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Kataoka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Tokuzo
authors.initials: T
authors.lastname: Arao
authors.forename: Sadako
authors.initials: S
authors.lastname: Nishimura
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nomura
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Chiyoda
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akahane
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishio
authors.forename: Kazuto
authors.initials: K
authors.lastname: Nishio
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000008:administration & dosage; D014408:Biomarkers, Tumor / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D018450:Disease Progression; D018572:Disease-Free Survival; D051766:Early Growth Response Protein 1 / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D017154:Stromal Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; C064269:FOSB protein, human; D016760:Proto-Oncogene Proteins c-fos