title: Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.
abstract: An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop acid (FA) was identified by HPLC/MS analysis. This strain converted FE by cleavage of the ester bond, but could not further degrade FA. Strain T1 could also efficiently degrade haloxyfop-R-methyl, quizalofop-p-ethyl, cyhalofop-butyl and clodinafop-propargyl. FE hydrolase capable of hydrolysing FE to FA was found in the cell-free extract of strain T1 by zymogram analysis. A novel gene feh encoding FE hydrolase was cloned by shotgun library construction and successfully expressed in Escherichia coli.
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hou
authors.forename: Jian
authors.initials: J
authors.lastname: Tao
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shen
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Jingquan
authors.initials: J
authors.lastname: Li
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Cui
mesh_terms: D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000378:metabolism*; D010802:Phylogeny; D011422:Propionates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology* / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010080:Oxazoles; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D002244:Carbon; C416661:fenoxaprop ethyl; D006867:Hydrolases