title: microRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2.
abstract: Deregulated microRNAs participate in carcinogenesis and cancer progression, but their roles in cancer development remain unclear. In this study, miR-365 expression was found to be downregulated in human colon cancer tissues as compared with that in matched non-neoplastic mucosa tissues, and its downregulation was correlated with cancer progression and poor survival in colon cancer patients. Functional studies revealed that restoration of miR-365 expression inhibited cell cycle progression, promoted 5-fluorouracil-induced apoptosis and repressed tumorigenicity in colon cancer cell lines. Furthermore, bioinformatic prediction and experimental validation were used to identify miR-365 target genes and indicated that the antitumor effects of miR-365 were probably mediated by its targeting and repression of Cyclin D1 and Bcl-2 expression, thus inhibiting cell cycle progression and promoting apoptosis. These results suggest that downregulation of miR-365 in colon cancer may have potential applications in prognosis prediction and gene therapy in colon cancer patients.
authors.affiliation: Department of Molecular Biology, PLA General Hospital, 28 Fuxing Road, Beijing 100853, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Du
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology* / Q000517:prevention & control; D019938:Cyclin D1 / Q000235:genetics*; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D019254:Genes, bcl-2; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology*
chemical_list: C530104:CCND1 protein, human; C546819:MIRN365 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1; D005472:Fluorouracil