title: Preliminary molecular identification of cylindrospermopsin-producing Cyanobacteria in two Polish lakes (Central Europe).
abstract: The presence of toxigenic cyanobacteria capable of biosynthesis of cylindrospermopsin (CYN) was measured in 24 water samples collected from the lakes Bytyńskie (BY) and Bnińskie (BN) in the Western Poland. The study also covered analysis of toxigenicity and production of CYN by the culture of Cylindrospermopsis raciborskii isolated from BY. The cyrJ gene associated with CYN production was identified in 22 water samples collected in the summer seasons of 2006 and 2007. The presence of CYN was confirmed in 16 samples. The homology searches revealed that amplified sequences of four water samples, which were selected from among all the samples, displayed a strong 99% homology to cyrJ gene of Aphanizomenon sp. 10E6. The culture of C. raciborskii did not contain the cyrJ gene nor the CYN. The specificity of C. raciborskii was confirmed by application of a fragment of the rpoC1. These first genetic analyses have shown that Aphanizomenon seems to be the main cyanobacterial genus responsible for the production of CYN in the Polish lakes. The lack of toxigenicity of the isolated C. raciborskii suggests that it is possible that this invasive species does not demonstrate toxigenic activity in Polish water bodies.
authors.affiliation: European Regional Centre for Ecohydrology u/a UNESCO PAS, Łódź, Poland. j.mankiewicz@erce.unesco.lodz.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Mankiewicz-Boczek
authors.forename: Mikołaj
authors.initials: M
authors.lastname: Kokociński
authors.forename: Ilona
authors.initials: I
authors.lastname: Gagała
authors.forename: Jakub
authors.initials: J
authors.lastname: Pawełczyk
authors.forename: Tomasz
authors.initials: T
authors.lastname: Jurczak
authors.forename: Jarosław
authors.initials: J
authors.lastname: Dziadek
mesh_terms: D000470:Alkaloids; D046890:Aphanizomenon / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001427:Bacterial Toxins; D001483:Base Sequence; D000087522:Cyanobacteria Toxins; D046891:Cylindrospermopsis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D011044:Poland; D012621:Seasons; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014498:Uracil / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D004269:DNA, Bacterial; C089595:cylindrospermopsin; D014498:Uracil