title: Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
abstract: A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Tessaro
authors.forename: Sameh S M
authors.initials: SS
authors.lastname: Soliman
authors.forename: Manish N
authors.initials: MN
authors.lastname: Raizada
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D005973:Glutamine / Q000032:analysis*; D049449:Luminescence; D009876:Operon; D010944:Plants / Q000737:chemistry*
chemical_list: D005973:Glutamine
references.citation: PLoS One. 2010 Jan 27;5(1):e8917
references.pmid: 20111724
references.citation: J Neurosci Methods. 2008 Jun 30;171(2):190-6
references.pmid: 18433876
references.citation: Amino Acids. 2007 Feb;32(2):203-12
references.pmid: 17031482
references.citation: Metab Eng. 2007 Jan;9(1):30-8
references.pmid: 17002894
references.citation: Appl Environ Microbiol. 1999 Jun;65(6):2685-90
references.pmid: 10347061
references.citation: Appl Environ Microbiol. 2001 Mar;67(3):1308-17
references.pmid: 11229926
references.citation: J Exp Bot. 2002 Apr;53(370):979-87
references.pmid: 11912240
references.citation: J Appl Microbiol. 1999 Jul;87(1):125-30
references.pmid: 10432593
references.citation: Plant Physiol. 1979 Mar;63(3):567-72
references.pmid: 16660768
references.citation: Mol Syst Biol. 2006;2:2006.0008
references.pmid: 16738554
references.citation: J Bacteriol. 1992 Aug;174(16):5371-81
references.pmid: 1644764
references.citation: Appl Environ Microbiol. 1994 Jun;60(6):1934-41
references.pmid: 16349283
references.citation: J Exp Bot. 2005 Feb;56(412):695-702
references.pmid: 15611144
references.citation: Plant Methods. 2008 Dec 31;4:28
references.pmid: 19117519
references.citation: J Microbiol Methods. 2000 May;40(3):207-12
references.pmid: 10802136
references.citation: Sci STKE. 2002 Jul 09;2002(140):pl10
references.pmid: 12107339
references.citation: Nat Genet. 2010 Dec;42(12):1060-7
references.pmid: 21037569
references.citation: Plant Cell. 2010 Nov;22(11):3509-42
references.pmid: 21081695