title: Phenol degradation activity and reusability of Corynebacterium glutamicum coated with NH(2)-functionalized silica-encapsulated Fe3O4 nanoparticles.
abstract: In this study, a novel method to immobilize and separate Corynebacterium glutamicum for phenol degradation was developed using Fe(3)O(4) nanoparticles (NPs). The Fe(3)O(4) NPs were encapsulated with silica and functionalized with NH(2) groups to enhance their capacity to adsorb on the cell surface. The results showed that the NH(2)-functionalized silica-encapsulated Fe(3)O(4) NPs strongly adsorbed on the cell surface of C. glutamicum during 32 d culture without any interruptions of their normal cell growth. The coated C. glutamicum were easily separated from the culture broth within 2 min by applying an external magnetic field Also, the coated C.glutamicum were able to completely degrade 50 ppm phenol in the culture broth after 8d culture at 30 °C. Concerning reusability, the coated cells could completely degrade phenol during the first 2 cycles, and retain ~60% activity of phenol degradation for the third and four cycles.
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu, Jeonju 561-756, South Korea.
authors.forename: Thai-Hoang
authors.initials: TH
authors.lastname: Le
authors.forename: So Jeong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seung Hyuck
authors.initials: SH
authors.lastname: Bang
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Choi
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000327:Adsorption; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism*; D059027:Recycling / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D005290:Ferric Compounds; D010636:Phenols; C000499:ferric oxide; D012822:Silicon Dioxide; D006859:Hydrogen; D009584:Nitrogen