title: Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
abstract: Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na⁺/H⁺ antiporter is investigated for the first time, by a combination of experimental and computational methodologies. We determined the protein's substrate specificity and pH dependence by fluorescence measurements in everted membrane vesicles. Subsequently, we constructed a model of the protein's structure and validated the model using molecular dynamics simulations. Taken together, better understanding of the Yersinia pestis Na⁺/H⁺ antiporter's structure-function relationship may assist in studies on ion transport, mechanism of action and designing specific blockers of Na⁺/H⁺ antiporter to help in fighting Yersinia pestis -associated infections. We hope that our model will prove useful both from mechanistic and pharmaceutical perspectives.
authors.affiliation: Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Assaf
authors.initials: A
authors.lastname: Ganoth
authors.forename: Raphael
authors.initials: R
authors.lastname: Alhadeff
authors.forename: Dovrat
authors.initials: D
authors.lastname: Kohen
authors.forename: Isaiah T
authors.initials: IT
authors.lastname: Arkin
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010930:Plague / Q000378:metabolism; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D015010:Yersinia pestis / Q000378:metabolism*
chemical_list: D017923:Sodium-Hydrogen Exchangers
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