title: Regulation of compound leaf development in Medicago truncatula by fused compound leaf1, a class M KNOX gene.
abstract: Medicago truncatula is a legume species belonging to the inverted repeat lacking clade (IRLC) with trifoliolate compound leaves. However, the regulatory mechanisms underlying development of trifoliolate leaves in legumes remain largely unknown. Here, we report isolation and characterization of fused compound leaf1 (fcl1) mutants of M. truncatula. Phenotypic analysis suggests that FCL1 plays a positive role in boundary separation and proximal-distal axis development of compound leaves. Map-based cloning indicates that FCL1 encodes a class M KNOX protein that harbors the MEINOX domain but lacks the homeodomain. Yeast two-hybrid assays show that FCL1 interacts with a subset of Arabidopsis thaliana BEL1-like proteins with slightly different substrate specificities from the Arabidopsis homolog KNATM-B. Double mutant analyses with M. truncatula single leaflet1 (sgl1) and palmate-like pentafoliata1 (palm1) leaf mutants show that fcl1 is epistatic to palm1 and sgl1 is epistatic to fcl1 in terms of leaf complexity and that SGL1 and FCL1 act additively and are required for petiole development. Previous studies have shown that the canonical KNOX proteins are not involved in compound leaf development in IRLC legumes. The identification of FCL1 supports the role of a truncated KNOX protein in compound leaf development in M. truncatula.
authors.affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA.
authors.forename: Jianling
authors.initials: J
authors.lastname: Peng
authors.forename: Jianbin
authors.initials: J
authors.lastname: Yu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.forename: Yingqing
authors.initials: Y
authors.lastname: Guo
authors.forename: Guangming
authors.initials: G
authors.lastname: Li
authors.forename: Guihua
authors.initials: G
authors.lastname: Bai
authors.forename: Rujin
authors.initials: R
authors.lastname: Chen
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004843:Epistasis, Genetic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics; D046913:Medicago truncatula / Q000235:genetics* / Q000254:growth & development*; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C097235:BEL1 protein, Arabidopsis; D018398:Homeodomain Proteins; D010940:Plant Proteins; D014157:Transcription Factors
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