title: The altered expression of α1 and β3 subunits of the gamma-aminobutyric acid A receptor is related to the hepatitis C virus infection.
abstract: The modulation of the gamma-aminobutyric acid type A (GABA A) receptors activity was observed in several chronic hepatitis failures, including hepatitis C. The expression of GABA A receptor subunits α1 and β3 was detected in peripheral blood mononuclear cells (PBMCs) originated from healthy donors. The aim of the study was to evaluate if GABA A α1 and β3 expression can also be observed in PBMCs from chronic hepatitis C (CHC) patients and to evaluate a possible association between their expression and the course of hepatitis C virus (HCV) infection. GABA A α1- and β3-specific mRNAs presence and a protein expression in PBMCs from healthy donors and CHC patients were screened by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. In patients, HCV RNA was determined in sera and PBMCs. It was shown that GABA A α1 and β3 expression was significantly different in PBMCs from CHC patients and healthy donors. In comparison to healthy donors, CHC patients were found to present an increase in the expression of GABA A α1 subunit and a decrease in the expression of β3 subunit in their PBMCs. The modulation of α1 and β3 GABA A receptors subunits expression in PBMCs may be associated with ongoing or past HCV infection.
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland. malgorzata.sidorkiewicz@umed.lodz.pl
authors.forename: M
authors.initials: M
authors.lastname: Sidorkiewicz
authors.forename: M
authors.initials: M
authors.lastname: Brocka
authors.forename: M
authors.initials: M
authors.lastname: Bronis
authors.forename: M
authors.initials: M
authors.lastname: Grek
authors.forename: B
authors.initials: B
authors.lastname: Jozwiak
authors.forename: A
authors.initials: A
authors.lastname: Piekarska
authors.forename: J
authors.initials: J
authors.lastname: Bartkowiak
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D019698:Hepatitis C, Chronic / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D021122:Protein Subunits / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000097:blood; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D021122:Protein Subunits; D012367:RNA, Viral; D011963:Receptors, GABA-A
references.citation: Am J Physiol Gastrointest Liver Physiol. 2003 Jul;285(1):G12-9
references.pmid: 12799308
references.citation: J Med Virol. 2006 Sep;78(9):1190-7
references.pmid: 16847959
references.citation: J Hepatol. 2000 Jan;32(1):85-91
references.pmid: 10673071
references.citation: World J Gastroenterol. 2008 May 14;14(18):2789-93
references.pmid: 18473399
references.citation: Gastroenterology. 2008 Mar;134(3):812-22
references.pmid: 18243182
references.citation: Clin Liver Dis. 2000 May;4(2):467-85
references.pmid: 11232201
references.citation: Neurogastroenterol Motil. 2008 Jun;20(6):671-9
references.pmid: 18282171
references.citation: J Gen Virol. 2005 Mar;86(Pt 3):657-666
references.pmid: 15722526
references.citation: J Virol. 2009 Jun;83(12):6211-21
references.pmid: 19357163
references.citation: J Immunol. 2004 Oct 15;173(8):5298-304
references.pmid: 15470076
references.citation: Hepatology. 2010 Sep;52(3):1060-7
references.pmid: 20812357
references.citation: Virus Res. 2009 Oct;145(1):141-4
references.pmid: 19540284
references.citation: Hepatology. 2011 Jan;53(1):53-61
references.pmid: 20967756
references.citation: Trends Mol Med. 2010 Jun;16(6):277-86
references.pmid: 20537953
references.citation: J Immunol. 2002 Sep 15;169(6):3447-58
references.pmid: 12218168
references.citation: Hepatology. 2007 Mar;45(3):735-45
references.pmid: 17326191
references.citation: World J Gastroenterol. 2007 Sep 28;13(36):4839-47
references.pmid: 17828815
references.citation: Hepatology. 2009 May;49(5):1431-41
references.pmid: 19177592
references.citation: Dig Liver Dis. 2007 Sep;39 Suppl 1:S38-45
references.pmid: 17936221
references.citation: J Neuroimmunol. 2008 Dec 15;205(1-2):44-50
references.pmid: 18954912
references.citation: Physiology (Bethesda). 2009 Jun;24:171-85
references.pmid: 19509127
references.citation: Antimicrob Agents Chemother. 2002 Oct;46(10):3280-2
references.pmid: 12234860
references.citation: Lancet. 1983 Oct 8;2(8354):811-4
references.pmid: 6137647
references.citation: Nature. 2008 Jan 24;451(7177):460-4
references.pmid: 18185516
references.citation: J Biol Chem. 2004 Oct 1;279(40):41422-35
references.pmid: 15258161
references.citation: PLoS One. 2011;6(6):e21328
references.pmid: 21712993
references.citation: Am J Physiol Gastrointest Liver Physiol. 2000 Oct;279(4):G733-9
references.pmid: 11005760
references.citation: Hepatol Res. 2008 Nov;38 Suppl 1:S116-21
references.pmid: 19125942
references.citation: Cereb Cortex. 2010 Aug;20(8):1974-84
references.pmid: 20034999
references.citation: Mol Immunol. 2006 Mar;43(9):1432-42
references.pmid: 16213022
references.citation: Gastroenterology. 1993 Jan;104(1):217-21
references.pmid: 8419244
references.citation: J Neurovirol. 2005 Jul;11(3):256-64
references.pmid: 16036805