title: [Effect of dietary fiber in the quantitative expression of butyrate receptor GPR43 in rats colon].
abstract: BACKGROUND
  Short chain fatty acids (SCFA) acetate, propionate and butyrate are the major anions produced by the bacterial fermentation of dietary fiber (DF) in colon. Recently, butyrate has been recently studied because is important to maintain colonic functions and because it has been related with a protective effect in colorectal cancer, which is mainly, explained by its potential to regulate gene expression by inhibiting enzyme histonedeacetylase (HDAC). Several investigationsshown that SCFAreceptor GPR43 is involved insignal transduction mechanisms once they bind to ligands such as butyrate to generate different physiological effects in colonocytes.
  
  
  OBJECTIVE
  Determine if dietary fiber consumption from nopal (Opuntia ficus I.) containing a ratio of soluble-insoluble fiber 40/60, has a direct influence on the quantitative expression of butyrate-specific receptor GPR43.
  
  
  METHODS
  Wistar rats were fed with four different diets formulated at different concentrations of dietary fiber of 0, 5, 15 and 25% of dietary fiber from opuntia, respectively.
  
  
  CONCLUSIONS
  The results shown an increase in the expression of GPR43 (93.1%) when rats was fed with a 5% fiber diet, using β-actin as a reference gene. The results of this investigation will contribute to determinate the relation of diet with intestinal health for the purpose of expanding the knowledge of butyric acid on colonic functions.
authors.affiliation: Programa Institucional de Maestría en Ciencias Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Mexico.
authors.forename: L Y
authors.initials: LY
authors.lastname: Corte Osorio
authors.forename: H E
authors.initials: HE
authors.lastname: Martínez Flores
authors.forename: R
authors.initials: R
authors.lastname: Ortiz Alvarado
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004032:Diet; D004043:Dietary Fiber / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation; D005453:Fluorescence; D008024:Ligands; D029747:Opuntia; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D000199:Actins; D002087:Butyrates; D004043:Dietary Fiber; C518630:Ffar2 protein, rat; D008024:Ligands; D043562:Receptors, G-Protein-Coupled; D012313:RNA