title: [Pulmonary lymphomatoid granulomatosis: an immunohistochemical and gene rearrangement study].
abstract: OBJECTIVE
  To study the immunophenotype and gene rearrangement pattern of pulmonary lymphomatoid granulomatosis.
  
  
  METHODS
  Nine cases of pulmonary lymphomatoid granulomatosis, included 5 cases of open lung biopsy, 3 cases of lobectomy specimen and 1 case of autopsy, were retrospectively analyzed by immunohistochemistry, in-situ hybridization for Epstein-Barr virus-encoded RNA, immunoglobulin and T-cell receptor gene rearrangement studies.
  
  
  RESULTS
  The age of patients ranged from 3 to 59 years. The male-to-female ratio was 3: 6. Histologically, all cases showed lymphocytic infiltration surrounding the blood vessels and in the perivascular areas. Most of these lymphoid cells expressed T-cell marker CD3. There were also variable numbers of CD20-positive B cells. The staining for CD56 was negative. According to the WHO classification, there were 4 cases of grade I , 1 case of grade II and 4 cases of grade III lesions. Six cases had gene rearrangement studies performed and 3 of them demonstrated clonal immunoglobulin gene rearrangement (including 1 of the grade II and 2 of the grade III lesions). No T-cell receptor gene rearrangement was detected.
  
  
  CONCLUSIONS
  Pulmonary lymphomatoid granulomatosis may represent a heterogeneous group of lymphoproliferative disorders. Some of the cases show B-cell immunophenotype and clonal immunoglobulin gene rearrangement, especially the grade II and grade lesions. They are likely of lymphomatous nature.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Rui-e
authors.initials: RE
authors.lastname: Feng
authors.forename: Hong-rui
authors.initials: HR
authors.lastname: Liu
authors.forename: Tong-hua
authors.initials: TH
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Ling
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Shi
authors.forename: Ding-rong
authors.initials: DR
authors.lastname: Zhong
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Cao
mesh_terms: D000328:Adult; D018951:Antigens, CD20 / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008230:Lymphomatoid Granulomatosis / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011013:Pneumonectomy / Q000379:methods; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D018951:Antigens, CD20; D017252:CD3 Complex