title: Internalization by osteoblasts of two Staphylococcus aureus clinical isolates differing in their adhesin gene pattern.
abstract: Staphylococcus aureus is the leading etiologic agent of implant orthopedic infections. Until recently S. aureus was considered a mere extracellular pathogen; it then turned out to be able to invade eukaryotic cells. Adhesion of S. aureus to peri-prosthesis tissues represents the starting of the infection pathogenesis and the first step of the subsequent internalization of S. aureus by host cells. In the present work the experimental observations on two epidemic clinical strains differing in their adhesin pattern demonstrate the crucial role of the fibronectin-binding protein A in the internalization process and suggest that CNA and Bbp adhesins can play a synergistic role by acting in the initial adhesion of S. aureus to osteoblasts, thus favoring the subsequent FnBPA-mediated internalization.
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, Bologna, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Testoni
authors.forename: Lucio
authors.initials: L
authors.lastname: Montanaro
authors.forename: Alessandro
authors.initials: A
authors.lastname: Poggi
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Davide
authors.initials: D
authors.lastname: Campoccia
authors.forename: Carla Renata
authors.initials: CR
authors.lastname: Arciola
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D010006:Osteoblasts / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C048165:adhesin, Staphylococcus aureus; C405490:bone sialoprotein-binding protein, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial