title: Comparative metabolism of warfarin in rats and chickens.
abstract: Warfarin, a coumarin rodenticide, is commonly used worldwide for rodent control, and is often reported as the cause for poisoning accidents in nontarget animals, in particular bird species. However, the metabolism of warfarin in birds is still unclear. In a previous study, we found an unknown warfarin metabolite in chicken cytosolic fractions. In the present study, we aimed to clarify the cytosolic warfarin metabolites in chickens compared with those in rats. The cytosol fractions of both chicken and rat livers showed the metabolic activity of 2 diastereomers and 2 enantiomers of warfarin alcohol. In chicken cytosol, we found that the production level of (S)-warfarin-(S)-alcohol was markedly higher (32-fold) than that in rat cytosol. From the results of the inhibition assay, we finally suggest that aldehyde oxidase may mainly contribute to the warfarin alcohol products in chicken cytosol.
authors.affiliation: Department of Environmental Veterinary Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Saengtienchai
authors.forename: Y
authors.initials: Y
authors.lastname: Ikenaka
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: M
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D051381:Rats; D012378:Rodenticides / Q000493:pharmacokinetics*; D014859:Warfarin / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D012378:Rodenticides; D014859:Warfarin