title: Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis.
abstract: OBJECTIVE
  To evaluate the antimicrobial efficacy of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis.
  
  
  METHODS
  One hundred and six single-rooted human teeth were inoculated with E. faecalis (ATCC 29212) and incubated in an microaerophilic environment for 21 days. Teeth were divided according to the medicaments and period: G1 - Calen (7 days); G2 - Calen (14 days); G3 - Calen/camphorated paramonochlorophenol (CMCP) (7 days); G4 - Calen/CMCP (14 days); G5 - Calen/0.4% chlorhexidine (CHX) (7 days); G6 - Calen/0.4% CHX (14 days); G7 - Calen/1% CHX (7 days); G8 - Calen/1% CHX (14 days); G9 - chemo-mechanical preparation with no medication and G10 - no chemo-mechanical preparation and no medication. Microbial samples were harvested immediately after removal of the intracanal dressing and 7 days later. Data were subjected to anova (P < 0.05).
  
  
  RESULTS
  All medicaments were associated with a reduction in E. faecalis after removal of the dressing (post-medication harvest). However, all specimens were associated with an increase in CFU mL(-1) values after 7 days (final harvest). Calen/CMCP and Calen/CHX had the lowest CFU mL(-1) values (P < 0.05).
  
  
  CONCLUSIONS
  In this laboratory study using extracted teeth all calcium hydroxide-based medicaments were able to significantly reduce the CFU mL(-1) values of E. faecalis in the root canal system.
authors.affiliation: Department of Restorative Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: R K P
authors.initials: RK
authors.lastname: Lima
authors.forename: J M
authors.initials: JM
authors.lastname: Guerreiro-Tanomaru
authors.forename: N B
authors.initials: NB
authors.lastname: Faria-Júnior
authors.forename: M
authors.initials: M
authors.lastname: Tanomaru-Filho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D058491:Bacterial Load / Q000187:drug effects; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002164:Camphor / Q000494:pharmacology; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects; D012387:Root Canal Filling Materials / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004338:Drug Combinations; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C420643:calen; D002164:Camphor; C029804:camphorated parachlorophenol; C010882:chlorhexidine gluconate; D002126:Calcium Hydroxide; D002710:Chlorhexidine