title: The role of speciation in positive Lowenstein-Jensen culture isolates from a high tuberculosis burden country.
abstract: OBJECTIVE
  To determine the need for routine speciation of positive Lowenstein-Jensen mycobacterial cultures in HIV-infected patients suspected of having pulmonary tuberculosis at Mulago Hospital in Kampala, Uganda.
  
  
  METHODS
  Sputum and bronchoalveolar lavage Lowenstein-Jensen mycobacterial culture isolates from consecutive, HIV-infected patients admitted to Mulago Hospital with 2 weeks or more of cough were subjected to IS6110 PCR and rpoB genetic analysis to determine the presence of Mycobacterium tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM).
  
  
  RESULTS
  Eighty (100%) mycobacterial cultures from 65 patients were confirmed to be members of MTBC. Subsequent analysis of the cultures from 54 patients by PCR and sequence analyses to identify co-infection with NTM confirmed the presence of MTBC as well as the presence of Micrococcus luteus (n = 4), Janibacter spp. (n = 1) and six cultures had organisms that could not be identified.
  
  
  CONCLUSIONS
  Presumptive diagnosis of tuberculosis on the basis of a positive Lowenstein-Jensen culture is sufficient in HIV-infected Ugandans suspected of having tuberculosis. Routine molecular confirmation of positive Lowenstein-Jensen cultures is unnecessary in this low resource setting.
authors.affiliation: Department of Medicine, Makerere University College of Health Sciences, Kampala, Uganda.
authors.forename: William
authors.initials: W
authors.lastname: Worodria
authors.forename: Jillian
authors.initials: J
authors.lastname: Anderson
authors.forename: Adithya
authors.initials: A
authors.lastname: Cattamanchi
authors.forename: J Lucian
authors.initials: JL
authors.lastname: Davis
authors.forename: Saskia
authors.initials: S
authors.lastname: den Boon
authors.forename: Alfred
authors.initials: A
authors.lastname: Andama
authors.forename: Samuel D
authors.initials: SD
authors.lastname: Yoo
authors.forename: Moses
authors.initials: M
authors.lastname: Joloba
authors.forename: Laurence
authors.initials: L
authors.lastname: Huang
authors.forename: Midori
authors.initials: M
authors.lastname: Kato-Maeda
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology*; D000328:Adult; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000453:epidemiology* / Q000382:microbiology; D014454:Uganda / Q000453:epidemiology
chemical_list: D017931:DNA Primers
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