title: Proliferation and differentiation of human tenocytes in response to platelet rich plasma: an in vitro and in vivo study.
abstract: Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet-rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma-clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human tenocyte in vitro. The advantage of using PRCR is that the platelet-derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human tenocytes in vitro. The expression of specific tenocyte markers were similar to conventional fetal bovine serum (FBS)-treated tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR-treated tenocytes in vivo as compared to 10% FBS-treated cells. Overall, our feasibility study has indicated that PRCR can induce human tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries.
authors.affiliation: Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford OX3 7LD, UK. goodthing1209@gmail.com
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Qiu
authors.forename: James
authors.initials: J
authors.lastname: Triffitt
authors.forename: Andrew
authors.initials: A
authors.lastname: Carr
authors.forename: Zhidao
authors.initials: Z
authors.lastname: Xia
authors.forename: Afsie
authors.initials: A
authors.lastname: Sabokbar
mesh_terms: D000328:Adult; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003018:Clot Retraction; D024042:Collagen Type I / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D058575:Decorin / Q000235:genetics / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D005260:Female; D005312:Fetal Blood; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000502:physiology*; D013710:Tendons / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D024042:Collagen Type I; D003470:Culture Media; D058575:Decorin; C546878:DCN protein, human; C093504:SCX protein, human