title: Advanced molecular biologic techniques in toxicologic disease.
abstract: The advancement of molecular biologic techniques and their capabilities to answer questions pertaining to mechanisms of pathophysiologic events have greatly expanded over the past few years. In particular, these opportunities have provided researchers and clinicians alike the framework from with which to answer clinical questions not amenable for elucidation using previous, more antiquated methods. Utilizing extremely small molecules, namely microRNA, DNA, protein, and nanoparticles, we discuss the background and utility of these approaches to the progressive, practicing physician. Finally, we consider the application of these tools employed as future bedside point of care tests, aiding in the ultimate goal of unsurpassed patient care.
authors.affiliation: Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA. jeanine.ward@umassmemorial.org
authors.forename: Jeanine
authors.initials: J
authors.lastname: Ward
authors.forename: Gyongyi
authors.initials: G
authors.lastname: Szabo
authors.forename: David
authors.initials: D
authors.lastname: McManus
authors.forename: Edward
authors.initials: E
authors.lastname: Boyer
mesh_terms: D064420:Drug-Related Side Effects and Adverse Reactions; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D055432:Metabolomics; D035683:MicroRNAs / Q000502:physiology; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics; D049490:Systems Biology
chemical_list: D035683:MicroRNAs
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