title: [The study of combining high-risk human papillomavirus types checking and cytologic test in the screening of cervical lesions].
abstract: OBJECTIVE
  To study the relationship between thinprep cytologic test and the types of human papilloma virus (HPV) infection in cervical precancerous lesion screening.
  
  
  METHODS
  To perform high-risk HPV types test in 1375 samples. Choose 256 positive samples to take thinprep cytologic test (TCT) and directed biopsies under colposcopy. Adopting two-channels real time PCR to genotype and quantify eight high risk HPV DNA (high risk types: HPV 16, 18, 45, 31; intermediate risk types: HPV 33, 52, 58, 67).
  
  
  RESULTS
  There are 256 positive samples in High risk HPV DNA test (18.62%). WNL rate for TCT is 16.41% (42/256), ASCUS and above rate for TCT is 83.59% (214/256). There is no statistically significant difference in the viral loads of HPV infection rate between the TCT negative patients and positive patients (P > 0.5). Positive correspondence rate for TCT and biopsy are 92.86% (39/42), 81.36% (48/59), 85.19% (23/27) and 9/10.
  
  
  CONCLUSIONS
  High-risk HPV types checking combined with TCT and biopsy can raise positive rate significantly. It should be used as a reliable method for early diagnosis in cervical cancer and CIN screening.
authors.affiliation: Hafei Women & Children's Healthcare Hospital, Anhui, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Xu
authors.forename: Zheng-xin
authors.initials: ZX
authors.lastname: Xie
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.forename: Wei-qin
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Qi-fa
authors.initials: QF
authors.lastname: Li
mesh_terms: D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003581:Cytodiagnosis / Q000379:methods*; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000821:virology