title: mRNA display-based selections using synthetic peptide and natural protein libraries.
abstract: mRNA display is a powerful in vitro selection technique that can be applied toward the identification of peptides or proteins with desired properties. The physical conjugation between a protein and its own RNA presents unique challenges in manipulating the displayed proteins in an RNase-free environment. This protocol outlines the generation of synthetic peptide and natural proteome libraries as well as the steps required for generation of mRNA-protein fusion libraries, in vitro selection, and regeneration of the selected sequences. The selection procedures for the identification of Ca(2+)-dependent, calmodulin-binding proteins from synthetic peptide and natural proteome libraries are presented.
authors.affiliation: Eshelman School of Pharmacy and Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Steve W
authors.initials: SW
authors.lastname: Cotten
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zou
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Bao-Cheng
authors.initials: BC
authors.lastname: Huang
authors.forename: Rihe
authors.initials: R
authors.lastname: Liu
mesh_terms: D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger
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