title: [Comparative study on effect of icariin and genistein on proliferation and mineralization of osteoblasts in vitro].
abstract: OBJECTIVE
  To investigated the effect of icariin and genistein on proliferation and mineralization of cultured rat osteoblasts (rat calvarial osteoblasts, ROB). And to contrast the pharmacological activity of icariin and genistein.
  
  
  METHODS
  Bone cells were obtained by enzyme digestion from the segregated neonatal SD rat skull, and were cultured in MEM containing 10% FBS which was changed after three days later. Serial subcultivation was proceeded when cells covered with 90% culture dish. The final action concentration of icariin and genistein were both 1 x 10(-5) mol x L(-1). Proliferation was analyzed by MTT on 96-well plates, while differentiation was analyzed on 24-well plates. Under the induced condition, the alkaline phosphatase activity, calcium salt sediment yield and osteocalcin were measured at the 3, 6, 9, 12 d. At 12th day, ALP staining, alizarin red staining and calcified nodule count were preceded. Total RNA was isolated at 0, 6, 12, 24, 48, 72 h. The gene expression of bFGF, IGF-1, Osterix and Runx-2 was analyzed by Real-time RT-PCR.
  
  
  RESULTS
  With the concentration of 1 x 10(-5) mol x L(-1), icariin and genistein have no significant effect on the ROB' s proliferation. The osteogenesis, ALP activity, calcium salt sediment yield and osteocalcin, calcified tubercle amount were significantly increased. And they enhanced the mRNA level of bFGF, IGF-1, Osterix and Runx-2. On the level of osteoblasts, the activity of icariin is stronger than that of genistein.
  
  
  CONCLUSIONS
  When the final concentration of icariin and genistein is 1 x 10(-5) mol x L(-1), they can significantly promoted ROB maturation. And on the level of osteoblasts, the activity of icariin is stronger than that of genistein.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China. mingleiguo8307@163.com
authors.forename: Leiguo
authors.initials: L
authors.lastname: Ming
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
authors.forename: Baofeng
authors.initials: B
authors.lastname: Ge
authors.forename: Huiping
authors.initials: H
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D005419:Flavonoids / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D005419:Flavonoids; C493717:Runx2 protein, rat; C495181:Sp7 protein, rat; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D019833:Genistein; D000469:Alkaline Phosphatase; C056599:icariin