title: Biomolecule-recognition gating membrane using biomolecular cross-linking and polymer phase transition.
abstract: We present for the first time a biomolecule-recognition gating system that responds to small signals of biomolecules by the cooperation of biorecognition cross-linking and polymer phase transition in nanosized pores. The biomolecule-recognition gating membrane immobilizes the stimuli-responsive polymer, including the biomolecule-recognition receptor, onto the pore surface of a porous membrane. The pore state (open/closed) of this gating membrane depends on the formation of specific biorecognition cross-linking in the pores: a specific biomolecule having multibinding sites can be recognized by several receptors and acts as the cross-linker of the grafted polymer, whereas a nonspecific molecule cannot. The pore state can be distinguished by a volume phase transition of the grafted polymer. In the present study, the principle of the proposed system is demonstrated using poly(N-isopropylacrylamide) as the stimuli-responsive polymer and avidin-biotin as a multibindable biomolecule-specific receptor. As a result of the selective response to the specific biomolecule, a clear permeability change of an order of magnitude was achieved. The principle is versatile and can be applied to many combinations of multibindable analyte-specific receptors, including antibody-antigen and lectin-sugar analogues. The new gating system can find wide application in the bioanalytical field and aid the design of novel biodevices.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kuroki
authors.forename: Taichi
authors.initials: T
authors.lastname: Ito
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ohashi
authors.forename: Takanori
authors.initials: T
authors.lastname: Tamaki
authors.forename: Takeo
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D008567:Membranes, Artificial; D058608:Nanopores; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D008567:Membranes, Artificial; D011108:Polymers; D001360:Avidin; C052970:poly-N-isopropylacrylamide; D001710:Biotin