title: Detection of enzymatically generated hydrogen peroxide by metal-based fluorescent probe.
abstract: We developed a metal-based fluorescent probe for H(2)O(2) called MBFh1, which has an iron complex as a reaction site for H(2)O(2) and a 3,7-dihydroxyphenoxazine derivative as the fluorescent reporter unit. The iron complex reacts quickly with H(2)O(2) to form oxidants, and then the oxidants convert the closely appended nonfluorescent 3,7-dihydroxyphenoxazine moiety to resorufin in an intramolecular fashion. The quick response to H(2)O(2) allows us to plot the enzymatic evolution of H(2)O(2). A combination of N-acetyl-3,7-dihydroxyphenoxazine and horseradish peroxidase has been frequently used to detect enzymatically generated H(2)O(2), but this method has interference with phenol derivatives. The use of MBFh1 overcomes this drawback.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hitomi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takeyasu
authors.forename: Takuzo
authors.initials: T
authors.lastname: Funabiki
authors.forename: Masahito
authors.initials: M
authors.lastname: Kodera
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D008670:Metals / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry / Q000378:metabolism; D019800:Phenol / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D008670:Metals; C105363:N-acetyl-3,7-dihydroxyphenoxazine; D010078:Oxazines; D019800:Phenol; C014180:resorufin; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase