title: Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme.
abstract: N-deoxyribosyltransferases are essential enzymes in the nucleotide salvage pathway of lactobacilli. They catalyze the exchange between the purine or pyrimidine bases of 2'-deoxyribonucleosides and free pyrimidine or purine bases. In general, N-deoxyribosyltransferases are referred to as cytoplasmic enzymes, although there is no experimental evidence for this subcellular localization. In this work, the subcellular localization of N-deoxyribosyltransferase II (NTD) from Lactobacillus fermentum was examined by subcellular fractionation, transmission electron microscopy, and fluorescence microscopy. Our results indicate that L. fermentum NTD are distributed not only in the cytoplasm but also on the cell wall surface, and further studies showed that surface-attached NTD can be released into the culture broth and conventional buffers.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Lin
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhang
authors.forename: Fangjie
authors.initials: F
authors.lastname: Zhu
authors.forename: Jingtan
authors.initials: J
authors.lastname: Su
authors.forename: Dong
authors.initials: D
authors.lastname: Fang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Guiyou
authors.initials: G
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
authors.forename: Rongqing
authors.initials: R
authors.lastname: Zhang
authors.forename: Hongzhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D005591:Chemical Fractionation; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D052197:Limosilactobacillus fermentum / Q000737:chemistry* / Q000201:enzymology*; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010430:Pentosyltransferases / Q000032:analysis*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D008565:Membrane Proteins; D010430:Pentosyltransferases; C420226:nucleoside deoxyribosyltransferase II