title: [Effect of genistein on MAPK signal pathway in the collagen-induced arthritis fibroblast-like synoviocytes].
abstract: OBJECTIVE
  To study the effect of genistein (Gen) on MAPK signal pathway in the CIA rat fibroblast-like synoviocytes (FLS).
  
  
  METHODS
  The rat model of collagen-induced arthritis (CIA) was established. The cultured FLS of CIA rats were divided using randomized method. The effects of Gen (at the concentration of 50, 100, and 200 micromol/L, respectively) on the proliferation of FLS in CIA rats using methyl thiazolyl tetrazolium (MTT) assay. Effects of Gen (at the concentration of 50, 100, and 200 pmol/L, respectively) on the expressions of extracellular signal-regulated kinase (ERK) and phosphorylated extracellular signal-regulated kinase (p-ERK) in the FLS of CIA rats were detected.
  
  
  RESULTS
  Gen could inhibit the proliferation of FLS in CIA rats. The FLS proliferation in the high dose Gen group at 72 h was only 1.10+/-0.04, significantly lower than that in the model group (2.12+/-0.03, P<0.01). Besides, after Gen's action on FLS, the expression of p-ERK was down-regulated. It was only 0.34+/-0.02 in the high dose Gen group, significantly lower than that in the model group (2.68+/-0.14, P<0.01). There was no change in the expression of ERK (P>0.05).
  
  
  CONCLUSIONS
  Gen could inhibit the proliferation of FLS in CIA rats. Its mechanism of action was mainly correlated to down-regulating the tyrosine kinase of MAPK signal transduction pathway and inhibiting phosphorylation of ERK.
authors.affiliation: Medical College, Yangzhou University, Jiangsu.
authors.forename: Xue-zeng
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei-gan
authors.initials: WG
authors.lastname: Shen
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism*; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D019833:Genistein; D048049:Extracellular Signal-Regulated MAP Kinases