title: Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives.
abstract: Chlamydomonas reinhardtii has many advantages compared with traditional systems for the molecular farming of recombinant proteins. These include low production costs, rapid scalability at pilot level, absence of human pathogens and the ability to fold and assemble complex proteins accurately. Currently, the successful expression of several proteins with pharmaceutical relevance has been reported from the nuclear and the chloroplastic genome of this alga, demonstrating its usefulness for biotechnological applications. However, several factors affect the level of recombinant protein expression in Chlamydomonas such as enhancer elements, codon dependency, sensitivity to proteases and transformation-associated genotypic modification. The present review outlines a number of strategies to increase protein yields and summarizes recent achievements in algal protein production including biopharmaceuticals such as vaccines, antibodies, hormones and enzymes with implications on health-related approaches. The current status of bioreactor developments for algal culture and the challenges of scale-up and optimization processes are also discussed.
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210 San Luis Potosí, SLP, Mexico. rosales.s@fcq.uaslp.mx
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
authors.forename: Luz María Teresita
authors.initials: LM
authors.lastname: Paz-Maldonado
authors.forename: Ruth Elena
authors.initials: RE
authors.lastname: Soria-Guerra
mesh_terms: D019149:Bioreactors; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004345:Drug Industry / Q000379:methods*; D004798:Enzymes / Q000096:biosynthesis; D006728:Hormones / Q000096:biosynthesis; D006801:Humans; D058086:Microalgae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D058634:Molecular Farming; D046549:Plantibodies / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014612:Vaccines
chemical_list: D004798:Enzymes; D006728:Hormones; D046549:Plantibodies; D011994:Recombinant Proteins; D014612:Vaccines
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