title: A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples.
abstract: A simple, rapid and high-throughput fluorescent polarization immunoassay (FPIA) for simultaneous determination of organophosphorus pesticides (OPs) using a broad-specificity monoclonal antibody was developed. The effects of tracer structure, tracer concentration, antibody dilution, methanol content and matrix effect on FPIA performance were studied. The FPIA can detect 5 OPs simultaneously with a limit of detection below 10 ng mL(-1). The time required for the equilibrium of antibody-antigen interaction was less than 10 min. The recovery from spiked vegetable and environmental samples ranged from 71.3% to 126.8%, with the coefficient of variations ranging from 3.5% to 14.5%. The developed FPIA was applied to samples, followed by confirmation with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis. The developed FPIA demonstrated good accuracy and reproducibility, and is suitable for rapid and high-throughput screening for OP contamination with high-efficiency and low cost.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Lei
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Shen
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Yang
authors.forename: Ksenia A
authors.initials: KA
authors.lastname: Maksimova
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Sun
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D016231:Fluorescence Polarization Immunoassay; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000276:immunology; D010575:Pesticides / Q000032:analysis* / Q000276:immunology; D014675:Vegetables / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D009943:Organophosphorus Compounds; D010575:Pesticides; D014867:Water