title: Control of cell attachment on pH-responsive chitosan surface by precise adjustment of medium pH.
abstract: The purpose of this study is to demonstrate pH-responsive chitosan is able to control cell behavior in response to small changes in environmental pH, which is at useful pH suitable for recovering cultured cells without additional enzymatic treatment and extensive washing steps. HeLa cells attached and spread well on chitosan at pH 6.99 and 7.20. When the pH was increased to 7.65, over 90% of cells would rapidly detached from chitosan surface within 1 h. Similarly, fibronectin adsorbed on chitosan at pH 7.20 also rapidly desorbed after increasing the medium pH. Most importantly and interestingly, medium pH adjustment could be facilitated by altering environment pCO(2). It was found over 80% of HeLa cells could be recovered from chitosan surface within 1 h and the viability of detached cells was more than 95% by transferring the culture plate from incubator to atmospheric condition. Additionally, chitosan substrate could effectively control attachment/detachment of various types of cells including cell lines HaCaT, H1299, NIH-3T3, and primary corneal fibroblasts, indicating the technology described here is easily reproducible and should be promising for controlling rapid fibronectin adsorption/desorption and cell attachment/detachment for tissue engineering applications.
authors.affiliation: Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Chen
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Chung
authors.forename: I-Jong
authors.initials: IJ
authors.lastname: Wang
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D051379:Mice; D013499:Surface Properties / Q000187:drug effects
chemical_list: D003470:Culture Media; D005353:Fibronectins; D048271:Chitosan