title: Development of industrially feasible concentrated 30% and 40% nanoemulsions for intravenous drug delivery.
abstract: Emulsions for parenteral nutrition loaded with drugs are used for optimized drug delivery, but in case of poorly oil soluble drugs, the injection volume can be too large when using commercial 10-20% oil emulsions. To reduce the injection volume, the feasibility of producing injectable, physically stable concentrated emulsions up to 40% oil content was investigated. Emulsions were made from fish oil, stabilized with egg lecithin, using high-pressure homogenization. Emulsions with oil contents of 10%-40% were investigated to assess basic correlations between increasing oil content, applied production pressures, homogenization cycles and resulting bulk droplet size, content of larger particles, zeta potential, viscosity and short-term stability. The observed correlations showed that in high-pressure homogenization, the contribution of the dispersive effect dominated the coalescence effect at low and Optimum production conditions for 30% and 40% nanoemulsions, i.e. 800 bar and 2 -3 homogenization cycles, were established on lab scale. These production conditions are industrially feasible. The obtained droplet sizes (about 200 nm) and the content of larger droplets were comparable to 10% commercial emulsions of parenteral nutrition, being important for in vivo tolerability and organ distribution. Despite the high oil concentration, the viscosity of the nanoemulsions was sufficiently low for injection. The short-term storage study showed physical stability for 1 month. A concentrated nanoemulsion base formulation from regulatory accepted excipients is now available, ready for loading with drugs.
authors.affiliation: Department of Pharmaceutics, Biopharmaceutics & NutriCosmetics, Freie Universität Berlin, Berlin, Germany.
authors.forename: R H
authors.initials: RH
authors.lastname: Müller
authors.forename: D
authors.initials: D
authors.lastname: Harden
authors.forename: C M
authors.initials: CM
authors.lastname: Keck
mesh_terms: D016503:Drug Delivery Systems; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D007275:Injections, Intravenous; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D014783:Viscosity
chemical_list: D004655:Emulsions; D005395:Fish Oils