title: Preparation and characterization of bioactive glass nanoparticles prepared by sol-gel for biomedical applications.
abstract: Bioactive glass nanoparticles (BG-NPs), based on both ternary (SiO(2)-CaO-P(2)O(5)) and binary (SiO(2)-CaO) systems, were prepared via an optimized sol-gel method. The pH of preparation and the effect of heat treatment temperature were evaluated, as well as the effect of suppressing P in the bioactivity ability of the materials. The morphology and composition of the BG-NPs were studied using FTIR, XRD and SEM. The bioactive character of these materials was accessed in vitro by analyzing the ability for apatite formation onto the surface after being immersed in simulated body fluid (SBF). XRD, EDX and SEM were used to confirm the bioactivity of the materials. The BG-NP effect on cell metabolic activity was assessed by seeding L929 cells with their leachables, proving the non-cytotoxicity of the materials. Finally the most bioactive BG-NPs developed (ternary system prepared at pH 11.5 and treated at 700 °C) were successfully combined with chitosan in the production of biomimetic nanocomposite osteoconductive membranes that could have the potential to be used in guided tissue regeneration.
authors.affiliation: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, P-4806-909 Taipas, Guimarães, Portugal. gisela.luz@dep.uminho.pt
authors.forename: Gisela M
authors.initials: GM
authors.lastname: Luz
authors.forename: João F
authors.initials: JF
authors.lastname: Mano
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D044367:Phase Transition; D017553:Phosphorus Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D010087:Oxides; D017553:Phosphorus Compounds; C012500:phosphorus pentoxide; D012822:Silicon Dioxide; D048271:Chitosan; C016538:lime