title: Enrichment of ANME-1 from Eckernförde Bay sediment on thiosulfate, methane and short-chain fatty acids.
abstract: The microorganisms involved in sulfate-dependent anaerobic oxidation of methane (AOM) have not yet been isolated. In an attempt to stimulate the growth of anaerobic methanotrophs and associated sulfate reducing bacteria (SRB), Eckernförde Bay sediment was incubated with different combinations of electron donors and acceptors. The organisms involved in AOM coupled to sulfate reduction (ANME-1, ANME-2, and Desulfosarcina/Desulfococcus) were monitored using specific primers and probes. With thiosulfate as sole electron acceptor and acetate, pyruvate or butyrate as the sole electron donor, ANME-1 became the dominant archaeal species. This finding suggests that ANME-1 archaea are not obligate methanotrophs and that ANME-1 can grow on acetate, pyruvate or butyrate.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Christian G
authors.initials: CG
authors.lastname: Jagersma
authors.forename: Roel J W
authors.initials: RJ
authors.lastname: Meulepas
authors.forename: Peer H A
authors.initials: PH
authors.lastname: Timmers
authors.forename: Agata
authors.initials: A
authors.lastname: Szperl
authors.forename: Piet N L
authors.initials: PN
authors.lastname: Lens
authors.forename: Alfons J M
authors.initials: AJ
authors.lastname: Stams
mesh_terms: D001105:Archaea / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development*; D001454:Baltic States; D061006:Bays / Q000382:microbiology*; D019641:DNA, Archaeal / Q000235:genetics; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D019015:Geologic Sediments / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D008697:Methane / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013440:Sulfides / Q000378:metabolism; D013885:Thiosulfates / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D019641:DNA, Archaeal; D005232:Fatty Acids, Volatile; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D013885:Thiosulfates; D008697:Methane