title: Lack of association of cadherin expression and histopathologic type, metastasis, or patient outcome in oropharyngeal squamous cell carcinoma: a tissue microarray study.
abstract: Altered cadherin expression is important for metastasis in many carcinomas including head and neck squamous cell carcinoma (SCC). We evaluated E- and N-cadherin expression specifically in oropharyngeal SCC and correlated this with clinical and pathologic features. Oropharyngeal SCC patients with clinical follow up information were identified from clinician databases from 1996 through 2007 and tissue microarrays created. Tumors had been previously typed histopathologically as keratinizing, non-keratinizing, or non-keratinizing with maturation, and had known p16 and human papillomavirus status, respectively. Immunohistochemistry was performed on the microarrays, and staining was evaluated for presence and intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong) both visually and also with digital image analysis software. Of 154 cases, E-cadherin was expressed in 152 (98.7%) and N-cadherin in 17 (11.5%). Neither E- nor N-cadherin expression was statistically significantly associated with histopathologic type (P = 0.082 and P = 0.228, respectively). E-cadherin staining intensity was not statistically significantly associated with nodal or distant metastasis, either visually or by image analysis, (P = 0.098 and P = 0.963 respectively) nor was N-cadherin (P = 0.228 and P = 0.935 respectively). Neither E- nor N-cadherin expression was associated with death from disease (P = 0.995; P = 0.964, respectively). E-cadherin is extensively expressed by oropharyngeal SCC, even the non-keratinizing type. Our results suggest that cadherin expression may not be a predictor for nodal or distant metastasis in these tumors. Mechanisms independent of cadherin expression may be important for metastases in oropharyngeal SCC.
authors.affiliation: Department of Pathology and Immunology, Division of Anatomic and Molecular Pathology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: O C
authors.initials: OC
authors.lastname: Ukpo
authors.forename: W L
authors.initials: WL
authors.lastname: Thorstad
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: J S
authors.initials: JS
authors.lastname: Lewis
mesh_terms: D000368:Aged; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality / Q000556:secondary*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000401:mortality / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D009959:Oropharyngeal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D016019:Survival Analysis
chemical_list: D015703:Antigens, CD; C050361:CDH2 protein, human; D015820:Cadherins
references.citation: Auris Nasus Larynx. 2004 Mar;31(1):35-41
references.pmid: 15041052
references.citation: J Craniomaxillofac Surg. 2007 Jan;35(1):1-9
references.pmid: 17296306
references.citation: Cancer Res. 1991 Dec 1;51(23 Pt 1):6328-37
references.pmid: 1933895
references.citation: Clin Cancer Res. 1996 Oct;2(10):1801-8
references.pmid: 9816133
references.citation: Am J Pathol. 1998 Aug;153(2):333-9
references.pmid: 9708792
references.citation: Cancer. 2008 Jul 1;113(1):97-107
references.pmid: 18473353
references.citation: J Cell Biol. 1987 May;104(5):1361-74
references.pmid: 3553211
references.citation: Head Neck. 2008 Nov;30(11):1475-82
references.pmid: 18704970
references.citation: J Cell Biol. 1993 Feb;120(3):757-66
references.pmid: 8425900
references.citation: Histopathology. 2011 Jun;58(7):1117-26
references.pmid: 21438909
references.citation: Acta Otorhinolaryngol Ital. 2009 Jun;29(3):119-26
references.pmid: 20140157
references.citation: Int J Cancer. 2005 May 1;114(5):779-90
references.pmid: 15609307
references.citation: Cancer Res. 2005 Mar 1;65(5):1783-91
references.pmid: 15753375
references.citation: Int J Cancer. 2006 Feb 15;118(4):821-31
references.pmid: 16152579
references.citation: Ann Otol Rhinol Laryngol. 2009 May;118(5):368-73
references.pmid: 19548387
references.citation: Int J Cancer. 1995 Dec 20;64(6):367-70
references.pmid: 8550236
references.citation: Int J Cancer. 1993 Oct 21;55(4):580-5
references.pmid: 8406985
references.citation: Am J Surg Pathol. 2003 Nov;27(11):1463-70
references.pmid: 14576481
references.citation: Laryngoscope. 2001 Oct;111(10):1842-6
references.pmid: 11801955
references.citation: Hepatogastroenterology. 1998 Nov-Dec;45(24):2437-42
references.pmid: 9951940
references.citation: Anticancer Res. 1999 Sep-Oct;19(5C):4401-5
references.pmid: 10650783
references.citation: Head Neck Pathol. 2009 Sep;3(3):186-94
references.pmid: 20596971
references.citation: N Engl J Med. 2010 Jul 1;363(1):24-35
references.pmid: 20530316
references.citation: Head Neck. 2009 Nov;31(11):1393-422
references.pmid: 19787782
references.citation: Anticancer Res. 1998 Nov-Dec;18(6A):4177-80
references.pmid: 9891464
references.citation: J Biol Chem. 1999 Jul 23;274(30):21409-15
references.pmid: 10409703
references.citation: Int J Cancer. 2003 Apr 10;104(3):336-44
references.pmid: 12569557
references.citation: Oncogene. 2004 Jan 15;23(2):350-8
references.pmid: 14724563
references.citation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):339-45
references.pmid: 16504868
references.citation: Mod Pathol. 2010 Feb;23(2):213-24
references.pmid: 19915524
references.citation: Oncol Rep. 2009 May;21(5):1223-33
references.pmid: 19360298