title: Purification and characterization of haloalkaline thermoactive, solvent stable and SDS-induced protease from Bacillus sp.: a potential additive for laundry detergents.
abstract: An extracellular haloalkaline, thermoactive, solvent stable, SDS-induced serine protease was purified and characterized from an alkali-thermo tolerant strain Bacillus sp. SM2014 isolated from reverse osmosis reject. The enzyme was purified to homogeneity with recovery of 54.4% and purity fold of 64. The purified enzyme was composed of single polypeptide of molecular mass about 71 kDa. The enzyme showed optimum activity at alkaline pH 10 and temperature 60°C. The km and Vmax for the enzyme was 0.57 mg/ml and 445.23 U/ml respectively. The enzyme showed novel catalytic ability at high pH (10), temperature (60°C) and salinity (3M). Moreover, the stability of enzyme in organic solvents (50% v/v) of logP ≥ 2 signified the prospective of this enzyme for peptide synthesis. The compatibility of the enzyme with surfactants and various detergent matrices together with wash performance test confirmed its potential applicability in laundry industry.
authors.affiliation: Discipline of Salt Marine and Inorganic Chemicals, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research (CSIR), GB Marg, Bhavnagar, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Jain
authors.forename: Imran
authors.initials: I
authors.lastname: Pancha
authors.forename: Sanjiv K
authors.initials: SK
authors.lastname: Mishra
authors.forename: Anupama
authors.initials: A
authors.lastname: Shrivastav
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects / Q000201:enzymology*; D057886:Bleaching Agents / Q000494:pharmacology; D002364:Caseins / Q000032:analysis; D003470:Culture Media / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D006219:Halogens / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007477:Ions; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000494:pharmacology; D054712:Salinity; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012997:Solvents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
chemical_list: D000468:Alkalies; D057886:Bleaching Agents; D002364:Caseins; D003470:Culture Media; D003902:Detergents; D006219:Halogens; D007477:Ions; D008670:Metals; D011480:Protease Inhibitors; D012997:Solvents; D012967:Sodium Dodecyl Sulfate; D010450:Endopeptidases