title: Next generation sequencing-based analysis of repetitive DNA in the model dioecious [corrected] plant Silene latifolia.
abstract: BACKGROUND
  Silene latifolia is a dioecious [corrected] plant with well distinguished X and Y chromosomes that is used as a model to study sex determination and sex chromosome evolution in plants. However, efficient utilization of this species has been hampered by the lack of large-scale sequencing resources and detailed analysis of its genome composition, especially with respect to repetitive DNA, which makes up the majority of the genome.
  
  
  RESULTS
  We performed low-pass 454 sequencing followed by similarity-based clustering of 454 reads in order to identify and characterize sequences of all major groups of S. latifolia repeats. Illumina sequencing data from male and female genomes were also generated and employed to quantify the genomic proportions of individual repeat families. The majority of identified repeats belonged to LTR-retrotransposons, constituting about 50% of genomic DNA, with Ty3/gypsy elements being more frequent than Ty1/copia. While there were differences between the male and female genome in the abundance of several repeat families, their overall repeat composition was highly similar. Specific localization patterns on sex chromosomes were found for several satellite repeats using in situ hybridization with probes based on k-mer frequency analysis of Illumina sequencing data.
  
  
  CONCLUSIONS
  This study provides comprehensive information about the sequence composition and abundance of repeats representing over 60% of the S. latifolia genome. The results revealed generally low divergence in repeat composition between the sex chromosomes, which is consistent with their relatively recent origin. In addition, the study generated various data resources that are available for future exploration of the S. latifolia genome.
authors.affiliation: Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Plant Molecular Biology, České Budějovice, Czech Republic. macas@umbr.cas.cz
authors.forename: Jiří
authors.initials: J
authors.lastname: Macas
authors.forename: Eduard
authors.initials: E
authors.lastname: Kejnovský
authors.forename: Pavel
authors.initials: P
authors.lastname: Neumann
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Andrea
authors.initials: A
authors.lastname: Koblížková
authors.forename: Boris
authors.initials: B
authors.lastname: Vyskot
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D029751:Silene / Q000235:genetics*
chemical_list: D018744:DNA, Plant
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