title: NY-ESO-1 expression and immunogenicity in prostate cancer patients.
abstract: BACKGROUND
  Prostate cancer is the second leading cause of men cancer-related death. Cancer immunotherapy has been investigated as a treatment which might be instituted at the point of detection of androgen-independent metastatic disease.
  
  
  OBJECTIVE
  to investigate the expression and humoral response against NYESO-1 in patients with prostate cancer (PC) and to analyze the relationship between expression of NY-ESO-1 and clinicopathological features.
  
  
  METHODS
  NY-ESO-1 mRNA in surgically resected PC and benign prostatic hyperplasia (BPH) were examined by reverse transcriptionpolymerase chain reaction. The antibody response to NY-ESO-1 was examined by enzyme-linked Elisa assay using recombinant NYESO-1 protein.
  
  
  RESULTS
  NY-ESO-1 mRNA was detected in 9 of 23 (39%) PC patients. Antibodies against NY-ESO-1 protein were detected in 12 of 23 (52%) sera of PC patients and in 5 of 9 (55%) of NY-ESO-1 expressing tumors. However, no mRNA copy or NY-ESO-1 antibodies were detected in all BPH patients tested.
  
  
  CONCLUSIONS
  The present study has demonstrated the expression of NY-ESO-1mRNA in prostate Cancer patients and NY-ESO-1 antibody production. Our data suggest that NY-ESO-1 could be used as a tumor marker and constitute a good candidate for vaccine-based immunotherapy for hormonal resistant prostate cancer patients.
authors.affiliation: Universite Tunis, El Manar, Tunisie.
authors.forename: Asma
authors.initials: A
authors.lastname: Gati
authors.forename: Nesrine
authors.initials: N
authors.lastname: Lajmi
authors.forename: Amine
authors.initials: A
authors.lastname: Derouiche
authors.forename: Raja
authors.initials: R
authors.lastname: Marrakchi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Chebil
authors.forename: Amel
authors.initials: A
authors.lastname: Benammar-Elgaaied
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000912:Antibodies, Neoplasm / Q000097:blood; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000276:immunology*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger