title: Specificities of Ricinus communis agglutinin 120 interaction with sulfated galactose.
abstract: Lectins are used extensively as research tools to detect and target specific oligosaccharide sequences. Ricinus communis agglutinin I (RCA(120)) recognizes non-reducing terminal β-D-galactose (Galβ) and its specificities of interactions with neutral and sialylated oligosaccharides have been well documented. Here we use carbohydrate arrays of sulfated Galβ-containing oligosaccharide probes, prepared from marine-derived galactans, to investigate their interactions with RCA(120). Our results showed that RCA(120) binding to Galβ1-4 was enhanced by 2-O- or 6-O-sulfation but abolished by 4-O-sulfation. The results were corroborated with competition experiments. Erythrina cristagalli lectin is also a Galβ-binding protein but it cannot accommodate any sulfation on Galβ.
authors.affiliation: Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, and Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Guangli
authors.initials: G
authors.lastname: Yu
authors.forename: Zhangrun
authors.initials: Z
authors.lastname: Han
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Yannan
authors.initials: Y
authors.lastname: Hu
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Jiandong
authors.initials: J
authors.lastname: Wu
authors.forename: Youjing
authors.initials: Y
authors.lastname: Lv
authors.forename: Wengang
authors.initials: W
authors.lastname: Chai
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D005685:Galactans / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D037121:Plant Lectins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism*
chemical_list: D005685:Galactans; D009844:Oligosaccharides; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D013431:Sulfates; C045629:erythrina lectin; D005690:Galactose