title: Study on pharmacokinetics and tissue distribution of pteryxin in mice by ultra-pressure liquid chromatography with tandem mass spectrometry.
abstract: Pteryxin is a coumarin compound naturally occurring in the roots of Radix Peucedani, a commonly used as traditional Chinese medicine for the treatment of certain respiratory diseases and hypertension. An UPLC-MS/MS method was established to quantify pteryxin in mouse plasma and tissue homogenates. Isoimperatorin was used as internal standard (IS). The method was based on protein precipitation with methanol for sample preparation. Pteryxin and IS were separated using a UPLC™ BEH C₁₈ column and eluted with a mobile phase consisting of methanol and water (70:30, v/v) at a flow-rate of 0.2 mL/min. MS/MS detection was carried out by monitoring the fragmentation of m/z 409.3-287.2 for pteryxin and m/z 271.3-185.2 for IS on a triple-quadrupole mass spectrometer. The total run time was only 6 min. The results showed that it had good linearity over a wide concentration range (r > 0.999), and pteryxin was rapidly distributed and then eliminated from mouse plasma (t(½) =1.463 h). The major distribution tissues of pteryxin in mice were liver, and pteryxin was enabled to cross the blood-brain barrier owing to its low polarity. There was no long-term accumulation of pteryxin in mouse tissues.
authors.affiliation: School of Pharmacy, Lanzhou University, 199 Donggangxi Road, Lanzhou 730000, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Ma
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.forename: Tongqiang
authors.initials: T
authors.lastname: Chen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shen
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002087:Butyrates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000097:blood / Q000493:pharmacokinetics*; D011564:Furocoumarins; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D016014:Linear Models; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D000432:Methanol; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D002087:Butyrates; D003374:Coumarins; D011564:Furocoumarins; C055542:isoimperatorin; C019546:pteryxin; C019475:2-methylbutanoic acid; D000432:Methanol