title: Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation.
abstract: The phenomenon of DNA phosphorothioation (DNA sulfur modification) is widespread among prokaryotes and may serve as a mechanism to restrict gene transfer among bacteria. DndE is one of five essential proteins that are required for the DNA phosphorothioation process. However, its exact biochemical role in sulfur modification of DNA remains unclear. In this study, the DndE protein homologue from Salmonella enterica serovar Cerro 87 was overexpressed, purified and crystallized. The crystals of the DndE protein diffracted to 2.7 Å resolution and belonged to space group P3(1)21. These results will facilitate detailed structural analysis of DndE and further elucidation of its biochemical function.
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Fukun
authors.initials: F
authors.lastname: Chen
authors.forename: Kui
authors.initials: K
authors.lastname: Lin
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Leyi
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Shi
authors.forename: Chunyang
authors.initials: C
authors.lastname: Cao
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingdan
authors.initials: J
authors.lastname: Liang
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Geng
authors.initials: G
authors.lastname: Wu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D010766:Phosphorylation; D019779:Salmonella enterica / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D013438:Sulfhydryl Compounds; D004247:DNA
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