title: Corynebacterium epidermidicanis sp. nov., isolated from skin of a dog.
abstract: A Gram-stain-positive, pleomorphic, oxidase-negative, non-motile isolate from the skin of a dog, designated strain 410(T), was subjected to comprehensive taxonomic characterization. Comparison of the 16S rRNA gene sequences revealed that the novel isolate showed highest similarities to the type strains of Corynebacterium humireducens, Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis and Corynebacterium ulcerans (96.1-96.8 %). The quinone system consisted predominantly of MK-8(H(2)) and MK-9(H(2)). The polar lipid profile of strain 410(T) contained the major compounds diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and four unidentified glycolipids. The polyamine pattern was composed of the major amines spermidine and spermine. In the fatty acid profile, predominantly straight-chain, saturated and mono-unsaturated fatty acids were detected (C(18 : 1)ω9c, C(16 : 1)ω7c, C(16 : 0)). These chemotaxonomic traits are in agreement with those reported for representatives of the genus Corynebacterium. Strain 410(T) tested negative for diphtheria toxin. Physiological properties as well as unique traits in the polar lipid profile could be used to distinguish strain 410(T) from the most closely related species. These data suggest that strain 410(T) represents a novel species of the genus Corynebacterium, for which we propose the name Corynebacterium epidermidicanis sp. nov. The type strain is 410(T) (= DSM 45586(T) = LMG 26322(T) = CCUG 60915(T)).
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Alexa
authors.initials: A
authors.lastname: Frischmann
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Knoll
authors.affiliation: Institut für Fleischhygiene, Fleischtechnologie und Lebensmittelwissenschaften, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Friederike
authors.initials: F
authors.lastname: Hilbert
authors.affiliation: Department of Bacteriology, National Institute of Public Health, National Institute of Hygiene, 00-791 Warsaw, Poland.
authors.forename: Aleksandra Anna
authors.initials: AA
authors.lastname: Zasada
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D003352:Corynebacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S