title: Isolation and characterization of a Klebsiella oxytoca strain for simultaneous azo-dye anaerobic reduction and bio-hydrogen production.
abstract: A facultative anaerobic bacteria strain GS-4-08, isolated from an anaerobic sequence batch reactor for synthetic dye wastewater treatment, was investigated for azo-dye decolorization. This bacterium was identified as a member of Klebsiella oxytoca based on Gram staining, morphology characterization and 16S rRNA gene analysis. It exhibited a good capacity of simultaneous decolorization and hydrogen production in the presence of electron donor. The hydrogen production was less affected even at a high Methyl Orange (MO) concentration of 0.5 mM, indicating a superior tolerability of this strain to MO. This efficient bio-hydrogen production from electron donor can not only avoid bacterial inhibition due to accumulation of volatile fatty acids during MO decolorization, but also can recover considerable energy from dye wastewater.
authors.affiliation: Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou 215123, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Michael Hon-Wah
authors.initials: MH
authors.lastname: Lam
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
mesh_terms: D000693:Anaerobiosis; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D007220:Industrial Waste; D041121:Klebsiella oxytoca / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid / Q000379:methods*; D014871:Water Microbiology
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D007220:Industrial Waste; C100258:methyl orange; D006859:Hydrogen