title: The protective role of ascorbic acid in burn-induced testicular damage in rats.
abstract: OBJECTIVE
  To investigate the ability of ascorbic acid to protect the testes from damage in severe burns.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University of Lagos Medical School, Department of Anatomy.
  
  
  METHODS
  28 adult male Wistar rats (250-300 g).
  
  
  METHODS
  Third degree burn was induced on 40% body surface area of rats and they were given ascorbic acid at 4 mg/kg over 8 weeks.
  
  
  METHODS
  Weight of reproductive organs and epididymal sperm parameters were measured. Oxidative status was assayed and a semi-quantitative assessment of histologic changes was also carried out.
  
  
  RESULTS
  Burn caused severe seminiferous tubular damage, especially germ cell loss (p<0.05). This was matched by significant reduction in sperm density and morphology (p<0.05). Burn also increased oxidative stress, with elevated malondialdehyde (MDA) levels (p<0.01) and changes in catalase and superoxide dismutase enzyme levels. Ascorbic acid prevented the changes in all sperm parameters. It normalized MDA levels (p<0.01) and attenuated changes in the levels of catalase and superoxide dismutase. Ascorbic acid treatment also significantly reduced histologic damage to seminiferous tubules.
  
  
  CONCLUSIONS
  This study shows that severe thermal injury causes significant testicular damage and impairs spermatogenesis. It also shows that ascorbic acid protects the testis from such damage and therefore has the potential to be a useful adjunct therapy during treatment of young males with severe burns.
authors.affiliation: Department of Anatomy, Lagos State University College of Medicine, PMB 21266, Ikeja, Lagos, Nigeria. petkutajewo07@yahoo.co.uk
authors.forename: P I
authors.initials: PI
authors.lastname: Jewo
authors.forename: F I
authors.initials: FI
authors.lastname: Duru
authors.forename: I O
authors.initials: IO
authors.lastname: Fadeyibi
authors.forename: L C
authors.initials: LC
authors.lastname: Saalu
authors.forename: C C
authors.initials: CC
authors.lastname: Noronha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D002056:Burns; D002374:Catalase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000473:pathology; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D000975:Antioxidants; D013739:Testosterone; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid