title: Corynebacterium glutamicum whcB, a stationary phase-specific regulatory gene.
abstract: The function of whcB, one of the four whiB homologues of Corynebacterium glutamicum, was assessed. Cells carrying the P(180)-whcB clone, and thus overexpressing the whcB gene, showed retarded growth, probably due to increased sensitivity to oxidants, whereas cells lacking whcB (ΔwhcB) did not. However, growth retardation was not observed in cells with additionally whcE deleted. Furthermore, the ΔwhcE phenotype, characterized by slow growth and sensitivity to oxidants, was reversed in cells carrying P(180)-whcB. Like the whcE gene, which is also known as a whiB homologue, the whcB gene was preferentially expressed in stationary phase. Determination of the genes under regulation of whcB using two-dimensional polyacrylamide gel electrophoresis identified several genes involved in electron transfer reactions that were regulated in cells carrying P(180)-whcB. Collectively, these findings indicate that whcB function requires whcE. Furthermore, whcB and whcE are paralogues but perform distinct regulatory roles during growth under oxidative stress.
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam, Korea.
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Joon-Song
authors.initials: JS
authors.lastname: Park
authors.forename: Hyung-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Younhee
authors.initials: Y
authors.lastname: Kim
authors.forename: Heung-Shick
authors.initials: HS
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D005809:Genes, Regulator; D018384:Oxidative Stress
chemical_list: D001426:Bacterial Proteins