title: A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model.
abstract: A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) targeting secreted antigen 1 of Babesia microti (BmSA1) was developed for detection of B. microti infection. The optimized DAS-ELISA was sensitive enough to detect circulating BmSA1 by day 2 post-infection, in sequential sera of a hamster infected with B. microti. This detection was 4 days earlier than antibody detection by indirect ELISA. The kinetics of circulating BmSA1 coincided with the profile of parasitemia. The specificity of this assay was evaluated using sera from animals experimentally infected with different species of Babesia. The DAS-ELISA had a higher sensitivity than the microscopic examination of Giemsa-stained blood smears for detection of the infection in hamsters. Taken together, these results indicated that BmSA1 could be a potential marker for surveillance of human babesiosis.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Mohamad Alaa
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Honglin
authors.initials: H
authors.lastname: Jia
authors.forename: Gabriel Oluga
authors.initials: GO
authors.lastname: Aboge
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.forename: Shinuo
authors.initials: S
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Longzheng
authors.initials: L
authors.lastname: Yu
authors.forename: Hideo
authors.initials: H
authors.lastname: Ooka
authors.forename: Ketsarin
authors.initials: K
authors.lastname: Kamyingkird
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Masatani
authors.forename: Shoufa
authors.initials: S
authors.lastname: Zhang
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000097:blood / Q000302:isolation & purification*; D041001:Babesia microti / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis; D015153:Blotting, Western; D002417:Cattle; D006224:Cricetinae; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006736:Horses; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008647:Mesocricetus; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera