title: Prevalence and molecular characterization of Mycoplasma ovis in selected free-ranging Brazilian deer populations.
abstract: Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Buffy coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer from Porto Primavera, 10/18 (56%) from Pantanal, and 4/21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 16S rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, 'Candidatus Mycoplasma erythrocervae', and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships.
authors.affiliation: Department of Veterinary Medicine, School of Veterinary Medicine, Universidade Federal do Paraná, Rua dos Funcionários, 1450, Curitiba, PR80035-050, Brazil.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Grazziotin
authors.forename: José Maurício Barbanti
authors.initials: JM
authors.lastname: Duarte
authors.forename: Matias Pablo Juan
authors.initials: MP
authors.lastname: Szabó
authors.forename: Andrea Pires
authors.initials: AP
authors.lastname: Santos
authors.forename: Ana Marcia Sa
authors.initials: AM
authors.lastname: Guimarães
authors.forename: Ahmed
authors.initials: A
authors.lastname: Mohamed
authors.forename: Rafael Felipe da Costa
authors.initials: RF
authors.lastname: Vieira
authors.forename: Ivan Roque
authors.initials: IR
authors.lastname: de Barros Filho
authors.forename: Alexander Welker
authors.initials: AW
authors.lastname: Biondo
authors.forename: Joanne Belle
authors.initials: JB
authors.lastname: Messick
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D001938:Brazil / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D003670:Deer / Q000382:microbiology*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012338:RNA, Ribosomal, 23S / Q000032:analysis; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S