title: Identification of proteins responding to adrenergic receptor subtype-specific hypertrophy in cardiomyocytes by proteomic approaches.
abstract: The individual signaling pathways underlying cardiac hypertrophy, which is induced by either α or β adrenergic receptor (AR), are different. Activation of different AR subtypes couples to different G proteins and induction of specific signaling pathways, which ultimately results in subtype-specific regulation of cardiac function. We present the first proteomics study identifying proteins that are related to AR subtype-specific hypertrophy in cardiomyocytes by comparing the two-dimensional electrophoresis patterns between neonatal rat cardiomyocytes treated by phenylepinephrin (PE) and by isoproterenol (ISO). An improved 2-DE strategy was used in these comparative experiments. Twenty-five differentially expressed proteins in cardiomyocytes treated by PE or treated by ISO were successfully analyzed and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry, especially those that might be responsible to intracellular oxidative stress such as dismutase, peroxiredoxin, and thioredoxin-like protein p46. In addition, induced reactive oxygen species were also found to be AR subtype-specifically relevant to endoplasmic reticulum proteinase ERK1/2 phosphorylation during the development of hypertrophy induced by different AR subtypes. The results will help to better understand the underlying mechanisms of different adrenergic receptor subtype-induced hypertrophy.
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun, PR China. liu_ning@jlu.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Kai-zheng
authors.initials: KZ
authors.lastname: Gong
authors.forename: Yuan-bin
authors.initials: YB
authors.lastname: Cai
authors.forename: Zijian
authors.initials: Z
authors.lastname: Li
mesh_terms: D000111:Acetylcysteine / Q000378:metabolism; D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced / Q000378:metabolism*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D007545:Isoproterenol / Q000737:chemistry; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018384:Oxidative Stress; D010656:Phenylephrine / Q000737:chemistry; D010766:Phosphorylation; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000235:genetics / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D011506:Proteins; D017382:Reactive Oxygen Species; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D010656:Phenylephrine; D007545:Isoproterenol; D000111:Acetylcysteine