title: Two-dimensional LC-MS fractioning and cross-matching of mass spectrometric data for rational identification of bioactive compounds in crude extracts.
abstract: Bioprospecting aims at the identification of biological compounds with novel properties. Identification of such compounds in crude complex biological extracts is a comprehensive challenge. As a large number of extracts must be screened for successful identification of one potential promising lead, rational screening strategies must be developed. Here we report on a novel two stage rational LC-MS strategy of extracts already pre-screened and proven to contain bioactive compound(s). All extracts are initially fractionated using one and the same LC condition with parallel mass spectrometric detection. Fractions containing bioactive compound(s) are then subjected to a second fractional stage using two different chromatographic conditions. Mass detection is also included at this stage, and a cross-matching algorithm for comparison of processed mass chromatograms from the two dimensions was developed. The algorithm reports only masses present in bioactive fractions in both dimensions and enable therefore an efficient identification of potential masses that causes the bioactivity. This mass list can be used to search in natural compound database(s) for a rapid evaluation if the mass belongs to an already identified compound or if it is a potentially new one. This strategy enables thorough screening of several hundred crude extracts in one week on one single instrument.
authors.affiliation: SINTEF Materials and Chemistry, Department of Biotechnology, Trondheim, Norway.
authors.forename: Geir
authors.initials: G
authors.lastname: Klinkenberg
authors.forename: Håvard
authors.initials: H
authors.lastname: Sletta
authors.forename: Espen
authors.initials: E
authors.lastname: Fjærvik
authors.forename: Kolbjørn
authors.initials: K
authors.lastname: Zahlsen
authors.forename: Per
authors.initials: P
authors.lastname: Bruheim
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry*; D001685:Biological Factors / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D001685:Biological Factors