title: [Isolation and identification of a Bacillus amyloliquefaciens YB-3 against Rhizoctonia solani].
abstract: OBJECTIVE
  An antagonistic bacterial strain YB-3 against Rhizoctonia solani was isolated from soils.
  
  
  METHODS
  Antagonistic strains were isolated by a reporter strain method. YB-3 was identified based on morphology observation, physiological and biochemical characterizations, Biolog, G + C content and 16S rDNA sequence analysis. The antagonistic spectrum and the properties of the inhibitor produced by Bacillus amyloliquefaciens YB-3 against plant pathogenic fungi and bacteria were investigated by means of plate two-way cultivation and disc diffusion method.
  
  
  RESULTS
  The strain YB-3 against Rhizoctonia solani was identified as Bacillus amyloliquefaciens. The antagonistic results showed that it had distinctively inhibitive effects on 14 pathogenic fungi and 7 bacteria. In addition, it also had inhibitive effects on strains from genus Bacillus to which YB-3 belongs. Antagonistic properties of B. amyloliquefaciens YB-3 was thermostable, acid resistant, and protease sensitive.
  
  
  CONCLUSIONS
  Bacillus amyloliquefaciens YB-3 was isolated and characterized which had distinctively inhibitive effects on Rhizoctonia solani and had broad-spectrum, highly efficient to plant pathogens.
authors.affiliation: Institute of Bioengineering of Henan University of Technology, Zhengzhou 450001, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Niu
authors.forename: Yuansen
authors.initials: Y
authors.lastname: Hu
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Yan
authors.forename: Haisheng
authors.initials: H
authors.lastname: Wang
mesh_terms: D000898:Antibiosis; D001407:Bacillus / Q000302:isolation & purification* / Q000502:physiology; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D012232:Rhizoctonia / Q000502:physiology*; D012988:Soil Microbiology