title: No role of homologous recombination in dealing with β-lapachone cytotoxicity in yeast.
abstract: β-Lapachone (β-lap) is a promising antitumoral agent. DNA base oxidation and alkylation are among the expected damages by β-lap. Herein, we have explored the role that the homologous recombination pathway (HR), a critical DNA repair process in Saccharomyces cerevisiae, has in the cytotoxic profile of β-lap. We have further compared β-lap to the closely related compound menadione and the well-known alkylating agent methyl methanesulfonate (MMS). Surprisingly, we found that β-lap does not trigger HR, as seen for (i) the mutant sensitivity profiles, (ii) concentration-dependent arrest profiles, (iii) absence of nuclear DNA repair factories, and (iv) frequency of recombination between direct repeats.
authors.affiliation: Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Carretera del Rosario 145, 38010 Santa Cruz de Tenerife, Spain.
authors.forename: Oliver
authors.initials: O
authors.lastname: Quevedo
authors.forename: Jonay
authors.initials: J
authors.lastname: García-Luis
authors.forename: Isabel
authors.initials: I
authors.lastname: Lorenzo-Castrillejo
authors.forename: Félix
authors.initials: F
authors.lastname: Machín
mesh_terms: D000478:Alkylation; D059447:Cell Cycle Checkpoints; D004247:DNA / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D059765:Homologous Recombination / Q000187:drug effects*; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009285:Naphthoquinones / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D024483:Vitamin K 3 / Q000633:toxicity
chemical_list: D009285:Naphthoquinones; C014638:beta-lapachone; D024483:Vitamin K 3; D004247:DNA; D008741:Methyl Methanesulfonate