title: Problem-solving test: submitochondrial localization of proteins.
abstract: Terms to be familiar with before you start to solve the test: mitochondria, outer membrane, inner membrane, intermembrane space, mitochondrial matrix, mitochondrial fraction, cell fractionation by differential centrifugation, pellet, supernatant, detergents, phenol, cytosolic fraction, integral and peripheral membrane proteins, hypotonic solution, SDS-polyacrylamide gel electrophoresis, Western blotting.
authors.affiliation: Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D015153:Blotting, Western; D002458:Cell Fractionation; D002498:Centrifugation; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007038:Hypotonic Solutions; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D008967:Molecular Biology / Q000193:education*; D018794:Problem-Based Learning / Q000379:methods*
chemical_list: D007038:Hypotonic Solutions; D024101:Mitochondrial Proteins