title: Catabolic pathway of gamma-caprolactone in the biocontrol agent Rhodococcus erythropolis.
abstract: Gamma-caprolactone (GCL) is well-known as a food flavor and has been recently described as a biostimulant molecule promoting the growth of bacteria with biocontrol activity against soft-rot pathogens. Among these biocontrol agents, Rhodococcus erythropolis, characterized by a remarkable metabolic versatility, assimilates various γ-butyrolactone molecules with a branched-aliphatic chain, such as GCL. The assimilative pathway of GCL in R. erythropolis was investigated by two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) analysis. This analysis suggests the involvement of the lactonase QsdA in ring-opening, a feature confirmed by heterologous expression in Escherichia coli. According to proteome analysis, the open-chain form of GCL was degraded by β- and ω-oxidation coupled to the Krebs cycle and β-ketoadipate pathway. Ubiquity of qsdA gene among environmental R. erythropolis isolates was verified by PCR. In addition to a previous N-acyl homoserine lactone catabolic function, QsdA may therefore be involved in an intermediate degradative step of cyclic recalcitrant molecules or in synthesis of flavoring lactones.
authors.affiliation: Laboratoire de Microbiologie Signaux et Microenvironnement, Normandie Université, EA 4312 Université de Rouen, IUT Evreux 55 rue Saint-Germain, 27000 Evreux, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Barbey
authors.forename: Alexandre
authors.initials: A
authors.lastname: Crépin
authors.forename: Amélie
authors.initials: A
authors.lastname: Cirou
authors.forename: Aurélie
authors.initials: A
authors.lastname: Budin-Verneuil
authors.forename: Nicole
authors.initials: N
authors.lastname: Orange
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Denis
authors.initials: D
authors.lastname: Faure
authors.forename: Yves
authors.initials: Y
authors.lastname: Dessaux
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Burini
authors.forename: Xavier
authors.initials: X
authors.lastname: Latour
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; D015107:4-Butyrolactone