title: Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour.
abstract: Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.
authors.affiliation: Faculty of Veterinary Medicine, Laboratory of Animal Pathology, University of São Paulo State, Araçatuba, São Paulo, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Stockmann
authors.forename: H F
authors.initials: HF
authors.lastname: Ferrari
authors.forename: A L
authors.initials: AL
authors.lastname: Andrade
authors.forename: T C
authors.initials: TC
authors.lastname: Cardoso
authors.forename: M C R
authors.initials: MC
authors.lastname: Luvizotto
mesh_terms: D000818:Animals; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D005260:Female; D005455:Fluorescent Antibody Technique; D016158:Genes, p53 / Q000235:genetics*; D008297:Male; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014685:Venereal Tumors, Veterinary / Q000235:genetics* / Q000473:pathology
chemical_list: D017931:DNA Primers; D010750:Phosphoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger