title: Effect of herbal preparation on heavy metal (cadmium) induced antioxidant system in female Wistar rats.
abstract: Cadmium is one of the elements found to damage antioxidant systems in mammals. To ameliorate cadmium toxicity and to prevent oxidative stress, natural products may be useful. In Indian ethnobotanical practice, a mixture of 17 herbal products is used to fortify the reproductive system of women after parturition and to reverse ovarian oxidative stress. Oral administration of this extract to rats exposed to cadmium was useful in reversing oxidative stress. Two different doses of cadmium (50 ppm and 200 ppm) were given to Wistar rats aged 45 and 65 days. An herbal extract derived from 17 plants was administered orally every day at a dose level of 200 mg/kg of body weight to the rats exposed to cadmium. A battery of enzymes involved in antioxidant activity in the ovary, including superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione-s-transferase (GST) were measured in the control, cadmium-exposed rats without treatment and in the cadmium-exposed rats treated with herbal extract. The reduction in SOD, catalase, GPx and GST activity after cadmium exposure improved significantly in the rats treated with the herbal extract (p < 0.05). The decrease of antioxidant enzymes due to cadmium exposure was reversed significantly with herbal extract administration. The synergistic effect of each bioactive compound in different herbal extracts requires further study.
authors.affiliation: Department of Zoology, Rani Anna Government College for Women, Tirunelveli, Tamil Nadu, India. dailiah.roopha@gmail.com
authors.forename: P
authors.initials: P
authors.lastname: Dailiah Roopha
authors.forename: C
authors.initials: C
authors.lastname: Padmalatha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D002104:Cadmium; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
references.citation: Phytomedicine. 2002 Mar;9(2):153-60
references.pmid: 11995949
references.citation: Toxicology. 2004 Jul 15;200(1):29-38
references.pmid: 15158561
references.citation: Environ Health Perspect. 1979 Feb;28:169-97
references.pmid: 226355
references.citation: Reprod Toxicol. 2003 Sep-Oct;17(5):553-60
references.pmid: 14555193
references.citation: J Assoc Physicians India. 2004 Oct;52:794-804
references.pmid: 15909857
references.citation: Indian J Physiol Pharmacol. 1996 Apr;40(2):155-8
references.pmid: 9062811
references.citation: Biochemistry. 1999 Aug 3;38(31):9872-80
references.pmid: 10433693
references.citation: Phytother Res. 2000 Aug;14(5):323-8
references.pmid: 10925395
references.citation: Biochem Mol Biol Int. 1997 Jun;42(1):35-44
references.pmid: 9192083
references.citation: Hum Exp Toxicol. 2011 Feb;30(2):91-101
references.pmid: 20203132
references.citation: Indian J Med Res. 1995 Jan;101:31-5
references.pmid: 7883281
references.citation: Biofactors. 2004;21(1-4):293-6
references.pmid: 15630214
references.citation: Eur J Haematol. 1993 Aug;51(2):86-92
references.pmid: 8370423
references.citation: J Environ Pathol Toxicol Oncol. 1998;17(3-4):259-64
references.pmid: 9726799
references.citation: Physiol Res. 2008;57(6):801-811
references.pmid: 18052675
references.citation: Pediatr Pharmacol (New York). 1983;3(1):49-55
references.pmid: 6646878
references.citation: Toxicol Appl Pharmacol. 1985 Aug;80(1):33-42
references.pmid: 4024106
references.citation: J Ethnopharmacol. 2003 Feb;84(2-3):251-8
references.pmid: 12648823
references.citation: Toxicology. 2000 Nov 16;153(1-3):83-104
references.pmid: 11090949
references.citation: Toxicol Appl Pharmacol. 1980 May;53(3):470-80
references.pmid: 6247787
references.citation: Biometals. 1998 Apr;11(2):153-7
references.pmid: 9542068
references.citation: Toxicol Appl Pharmacol. 1987 Jan;87(1):102-10
references.pmid: 3798447
references.citation: Science. 1973 Feb 9;179(4073):588-90
references.pmid: 4686466
references.citation: Indian J Exp Biol. 2000 Jun;38(6):604-6
references.pmid: 11116533
references.citation: Eur J Biochem. 1974 Sep 16;47(3):469-74
references.pmid: 4215654
references.citation: Toxicology. 2002 Sep 30;179(1-2):37-50
references.pmid: 12204541
references.citation: Vet Hum Toxicol. 1999 Apr;41(2):65-7
references.pmid: 10192131
references.citation: Indian J Exp Biol. 2003 Feb;41(2):135-40
references.pmid: 15255605
references.citation: J Biol Chem. 1974 Nov 25;249(22):7130-9
references.pmid: 4436300
references.citation: Physiol Res. 2003;52(5):563-70
references.pmid: 14535831
references.citation: J Ethnopharmacol. 2000 Sep;72(1-2):299-304
references.pmid: 10967486
references.citation: Arch Biochem Biophys. 1997 Oct 15;346(2):171-9
references.pmid: 9343363
references.citation: Biomed Environ Sci. 2008 Feb;21(1):24-9
references.pmid: 18478975
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Cochrane Database Syst Rev. 2000;(2):CD000980
references.pmid: 10796569
references.citation: Anal Biochem. 1972 Jun;47(2):389-94
references.pmid: 4556490