title: A specific probe for two-photon fluorescence lysosomal imaging.
abstract: Lysosomes are vital organelles in physiological processes, as they receive and degrade macromolecules from the secretory and endocytic procedures. Evidences have shown that lysosomes were related to oncogenic activation and cancer progression, so lysosomes targeting and imaging probes make them convenient to be observed. In this study, a lysosome specific probe W-7 was designed and synthesized via convenient one-pot reaction and Heck reaction. This probe was derived from Tröger's base with a dimethylaminomethyl end group. The optical properties of this compound were measured. W-7 also showed two-photon absorption (TPA) effect by using laser excitation at the wavelength of infrared light. In vivo experiment, W-7 showed high specificity and selectivity for lysosomes in living cells (HeLa cells, MRC-5 cells and NRK cells), compared with LT Red, GT Red and MT Red (R=0.96). Two-photon fluorescence images of HeLa cells stained by W-7 were obtained. And high resolution 3D reconstruction of lysosomes in one HeLa cell was provided by using two-photon confocal microscopy. The anantioseparation of racemic W-7 was carried out by chiral-HPLC, and the two enantiomers showed no significant difference in lysosomes imaging.
authors.affiliation: State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Hubei, Wuhan, PR China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wu
authors.forename: Mingliang
authors.initials: M
authors.lastname: Tang
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Deng
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Dong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tan
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000648:ultrastructure*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes