title: Proteomics-based investigation in C2C12 myoblast differentiation.
abstract: Skeletal muscle cell differentiation is a multistage process extensively studied over the years. Even if great improvements have been achieved in defining biological process underlying myogenesis, many molecular mechanisms need still to be clarified.To further highlight this process, we studied cells at undifferentiated, intermediate and highly differentiated stages, and we analyzed, for each condition, morphological and proteomic changes. We also identified the proteins that showed statistical significant changes by a ESI-Q-TOF mass spectrometer. This work provides further evidence of the involvement of particular proteins in skeletal muscle development. Furthermore, the high level of expression of many heat shock proteins, suggests a relationship between differentiation and cellular stress. Intriguingly, the discovery of myogenesis-correlated proteins, known to play a role in apoptosis, suggests a link between differentiation and this type of cell death.
authors.affiliation: Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino "Carlo Bo", Italy.
authors.forename: L
authors.initials: L
authors.lastname: Casadei
authors.forename: L
authors.initials: L
authors.lastname: Vallorani
authors.forename: A M
authors.initials: AM
authors.lastname: Gioacchini
authors.forename: M
authors.initials: M
authors.lastname: Guescini
authors.forename: S
authors.initials: S
authors.lastname: Burattini
authors.forename: A
authors.initials: A
authors.lastname: D'Emilio
authors.forename: L
authors.initials: L
authors.lastname: Biagiotti
authors.forename: E
authors.initials: E
authors.lastname: Falcieri
authors.forename: V
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D024510:Muscle Development / Q000502:physiology; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D040901:Proteomics / Q000379:methods*
chemical_list: D006360:Heat-Shock Proteins
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