title: Function and expression of renal epithelial sodium transporters in rats with thyroid dysfunction.
abstract: Thyroid disorders are accompanied by major changes in renal sodium handling and blood pressure. Sodium transporters play a crucial role in regulating sodium excretion. We determined the function and expression of type 3 Na/H (NHE3) exchanger, type 2 Na+K+2Cl co-transporter (NKCC2) co-transporter, NaCl co-transporter (NCC) cotransporter, and epithelial sodium channel (ENaC) in hypoand hyperthyroid rats at 6 weeks after each thyroid disorder induction. We measured the renal response to functional blockade of the tubular sodium transporters, using acetazolamide to inhibit the activity of NHE3, furosemide for NKCC2, hydrochlorotiazide for NCC, and amiloride for ENaC. Expression of sodium transporters was analyzed by measuring the protein abundance by Western blot. The responsiveness to NHE3 inhibition and NHE3 protein was lower in hypothyroid rats and higher in hyperthyroid rats vs controls. Hypothyroid rats showed greater diuretic and natriuretic responses to NKCC2 and ENaC blockade and higher protein abundance of NKCC2 vs controls. Hyperthyroid rats showed greater protein expression of NKCC2 and NCC vs controls. Groups did not differ in responsiveness to NCC blockade. The expression and activity of ENaC were lower in hyperthyroid rats. In conclusion, reduced NHE3 activity may participate in the low blood pressure of hypothyroid rats and elevated NHE3 activity in the high blood pressure of hyperthyroid rats. These proximal alterations are counter-balanced by functional upregulation of NKCC2 and ENaC in downstream nephron segments of hypothyroid rats and by downregulation of αENaC activity and expression in hyperthyroid rats.
authors.affiliation: Experimental Unit, Service of Nephrology, Department of Physiology, Virgen de las Nieves Hospital, Granada, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Moreno
authors.forename: R
authors.initials: R
authors.lastname: Peréz-Abud
authors.forename: R
authors.initials: R
authors.lastname: Wangensteen
authors.forename: I
authors.initials: I
authors.lastname: Rodríguez Gómez
authors.forename: I
authors.initials: I
authors.lastname: López Merino
authors.forename: A
authors.initials: A
authors.lastname: Osuna
authors.forename: F
authors.initials: F
authors.lastname: Vargas
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D053503:Epithelial Sodium Channels / Q000737:chemistry / Q000378:metabolism*; D006980:Hyperthyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D049992:Sodium Chloride Symporters / Q000737:chemistry / Q000378:metabolism*; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters / Q000737:chemistry / Q000378:metabolism*; D064466:Solute Carrier Family 12, Member 1
chemical_list: D053503:Epithelial Sodium Channels; C578716:Slc12a1 protein, rat; C000613387:Slc9a3 protein, rat; D049992:Sodium Chloride Symporters; D000074083:Sodium-Hydrogen Exchanger 3; D017923:Sodium-Hydrogen Exchangers; D028021:Sodium-Potassium-Chloride Symporters; D064466:Solute Carrier Family 12, Member 1