title: BK virus infection in human immunodeficiency virus-infected patients.
abstract: The aim of this study is to evaluate the prevalence of BK virus (BKV) infection in HIV-positive patients receiving highly active antiretroviral therapy (HAART) in our hospital. The presence of BKV was analysed in urine and plasma samples from 78 non-selected HIV-infected patients. Clinical data were recorded using a pre-established protocol. We used a nested PCR to amplify a specific region of the BKV T-large antigen. Positive samples were quantified using real-time PCR. Mean CD4 count in HIV-infected patients was 472 cells/mm3 and median HIV viral load was <50 copies/mL. BKV viraemia was detected in only 1 HIV-positive patient, but 57.7% (45 out of 78) had BKV viruria, which was more common in patients with CD4 counts>500 cells/mm3 (74.3% vs 25.7%; p=0.007). Viruria was present in 21.7% of healthy controls (5 out of 23 samples, p=0.02). All viral loads were low (<100 copies/mL), and we could not find any association between BKV infection and renal or neurological manifestations. We provide an update on the prevalence of BKV in HIV-infected patients treated with HAART. BKV viruria was more common in HIV-infected patients; however, no role for BKV has been demonstrated in this population.
authors.affiliation: Department of Clinical Microbiology-Infectious Diseases, Hospital General Universitario Gregorio Marañón, Doctor Esquerdo 46, 28007, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Ledesma
authors.forename: P
authors.initials: P
authors.lastname: Muñoz
authors.forename: D
authors.initials: D
authors.lastname: Garcia de Viedma
authors.forename: I
authors.initials: I
authors.lastname: Cabrero
authors.forename: B
authors.initials: B
authors.lastname: Loeches
authors.forename: P
authors.initials: P
authors.lastname: Montilla
authors.forename: P
authors.initials: P
authors.lastname: Gijon
authors.forename: B
authors.initials: B
authors.lastname: Rodriguez-Sanchez
authors.forename: E
authors.initials: E
authors.lastname: Bouza
mesh_terms: D000328:Adult; D000368:Aged; D001739:BK Virus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D011446:Prospective Studies; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000821:virology; D014556:Urine / Q000821:virology; D019562:Viral Load
chemical_list: D004279:DNA, Viral
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