title: Separation of lipid classes by HPLC on a cyanopropyl column.
abstract: A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.
authors.affiliation: Department of Analytical Chemistry, Stockholm University, Svante Arrhenius väg 16, 10691 Stockholm, Sweden.
authors.forename: Petter
authors.initials: P
authors.lastname: Olsson
authors.forename: Jan
authors.initials: J
authors.lastname: Holmbäck
authors.forename: Bengt
authors.initials: B
authors.lastname: Herslöf
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006536:Heptanes / Q000737:chemistry; D006586:Hexanes / Q000737:chemistry; D009795:Octanes / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D014050:Toluene / Q000737:chemistry
chemical_list: D006536:Heptanes; D006586:Hexanes; D009795:Octanes; D010743:Phospholipids; D014050:Toluene; C045798:2,2,4-trimethylpentane
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