title: [Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain].
abstract: OBJECTIVE
  To investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain.
  
  
  METHODS
  The neuropathic pain model was established by chronic constriction injury (CCI) 7 days before electrophysiological-recording. The rat DRG neurons were enzymatically dissociated. Whole-cell patch clamp technique was used to record GABA-activated currents. The changes of currents of injured side and opposite side were expected to compare with control group.
  
  
  RESULTS
  (1) The currents of injured side of CCI group were notablely decreased compared with control group (GABA concentration, 0.1-1000 micromol/L). (2) By the contrast, opposite side currents of CCI group increased significantly compared with those in injured side and control group (GABA concentration, 0.01-1000 micromol/L).
  
  
  CONCLUSIONS
  The data indicates that the chronic constriction injury change both the function of GABAA receptors of injury side and opposite side. The decrease of pre-synaptic inhibition of GABA may be the possible reason of neuropathic pain.
authors.affiliation: The key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University Medical College, Shihezi 832002, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhu
authors.forename: Ke-tao
authors.initials: KT
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Zhong-shuang
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jun-qiang
authors.initials: JQ
authors.lastname: Si
mesh_terms: D000818:Animals; D002469:Cell Separation; D003250:Constriction; D005727:Ganglia, Spinal / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009437:Neuralgia / Q000209:etiology / Q000503:physiopathology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism / Q000502:physiology*; D012584:Sciatic Nerve / Q000293:injuries
chemical_list: D011963:Receptors, GABA-A