title: Changes in protein expression in the sheep abomasum following trickle infection with Teladorsagia circumcincta.
abstract: Continual low-level exposure of sheep to the helminth Teladorsagia circumcincta elicits a temporary protective immunity, where factors in the immune abomasal mucosa prevent penetration of infective larvae, but which is essentially lost within 6 weeks of cessation of parasite challenge. Here, a proteomic approach was used to identify proteins that are differentially regulated in immune compared to naïve sheep, as potential key mediators of immunity. Six naïve sheep and 12 sheep trickle-infected with T. circumcincta were treated with anthelmintic, and the naïve (control) and 6 immune sheep were killed 7 days later. The remaining 6 sheep (immune waning) were killed 42 days after anthelmintic treatment. Abomasal tissue samples were subjected to 2D-gel electrophoresis and densitometric analysis. Selected spots (n=73) were identified by peptide mass fingerprinting and confirmatory Western blotting was carried out for 10 proteins. Spots selectively up-regulated in immune versus control, but not immune waning versus control sheep, included galectin-15 and thioredoxin, which were confirmed by Western blotting. In immune sheep, serum albumin was significantly down-regulated and albumin proteolytic cleavage fragments were increased compared to controls. Unexpectedly, albumin mRNA was relatively highly expressed in control mucosa, down-regulated in immune, and was immunolocalized to mucus-producing epithelial cells. Thus we have identified differential expression of a number of proteins following T. circumcincta trickle infection that may play a role in host protection and inhibition of parasite establishment.
authors.affiliation: The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK. alan.pemberton@ed.ac.uk
authors.forename: Alan D
authors.initials: AD
authors.lastname: Pemberton
authors.forename: Jeremy K
authors.initials: JK
authors.lastname: Brown
authors.forename: Nicky M
authors.initials: NM
authors.lastname: Craig
authors.forename: Judith
authors.initials: J
authors.lastname: Pate
authors.forename: Kevin
authors.initials: K
authors.lastname: McLean
authors.forename: Neil F
authors.initials: NF
authors.lastname: Inglis
authors.forename: David
authors.initials: D
authors.lastname: Knox
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Knight
mesh_terms: D000018:Abomasum / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D056704:Adaptive Immunity; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D037161:Galectins / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D034561:Sheep, Domestic; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism; D014251:Trichostrongyloidea; D014252:Trichostrongyloidiasis / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*
chemical_list: D000871:Anthelmintics; D037161:Galectins; D015801:Helminth Proteins; D012333:RNA, Messenger; D012709:Serum Albumin; D013879:Thioredoxins