title: Immunogenicity of eight Mycobacterium avium subsp. paratuberculosis specific antigens in DNA vaccinated and Map infected mice.
abstract: Mycobacterium avium subsp. paratuberculosis (Map), the etiological agent of chronic enteritis of the small intestine in domestic and wild ruminants, causes substantial losses to livestock industry. Control of this disease is seriously hampered by the lack of adequate diagnostic tools and vaccines. Here we report on the immunogenicity of eight Map specific antigens, i.e. MAP1693c, Ag3, MAP2677c (identified by post-genomic and immunoproteomic analysis of Map secretome) and Ag5, Ag6, MAP1637c, MAP0388 and MAP3743 (identified by bioinformatic in silico screening of the Map genome). Strong, antigen-specific IFN-γ responses were induced in mice vaccinated with plasmid DNA encoding MAP1693c, MAP1637c, MAP0388 and MAP3743. In contrast, T cell responses in Map infected mice were directed preferentially against Ag5 and to a lesser extent against MAP3743. None of the tested DNA vaccines conferred protection against subsequent challenge with Map.
authors.affiliation: Scientific Service Immunology, Scientific Institute of Public Health WIV-ISP (Site Ukkel), 642 Engelandstraat, 1180 Brussels, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Roupie
authors.forename: Sophie
authors.initials: S
authors.lastname: Viart
authors.forename: Baptiste
authors.initials: B
authors.lastname: Leroy
authors.forename: Marta
authors.initials: M
authors.lastname: Romano
authors.forename: Nicolas
authors.initials: N
authors.lastname: Trinchero
authors.forename: Marc
authors.initials: M
authors.lastname: Govaerts
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Letesson
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.forename: Kris
authors.initials: K
authors.lastname: Huygen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010283:Paratuberculosis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007376:Interleukin-2; D019444:Vaccines, DNA; D007371:Interferon-gamma