title: Inter-population comparisons of copper resistance and accumulation in the red seaweed, Gracilariopsis longissima.
abstract: Copper (Cu) resistance and accumulation of five populations of the red seaweed Gracilariopsis longissima collected from sites in south west England (Fal Estuary, Helford Estuary and Chesil Fleet) that differ in their degree of Cu contamination was assessed under controlled laboratory conditions, on two separate occasions (April and October). The effects of a range of Cu concentrations (0-250 μg l(-1)) on relative growth rates was the same for all populations with reductions observable at concentrations as low as 12 μg l(-1) and cessation of growth at 250 μg l(-1). There was no significant difference in the calculated EC(50) values for the April and October samples, with means of 31.1 and 25.8 μg l(-1), respectively. Over the range of concentrations used in this study, copper content increased linearly and the pattern of accumulation was the same for all populations at both time periods. From the linear regressions of the pooled data a concentration factor of 2.25 × 10(3) was calculated. These results imply that G. longissima has an innate tolerance to Cu and that populations have not evolved copper-tolerant ecotypes. In laboratory studies, accumulated Cu was released when transferred to 'clean' seawater with approximately 80% being lost after 8 days, with no significant difference between populations in their response. The results from a 30 days in situ transplantation experiment using two populations from the Fal Estuary provided further evidence for dynamic changes in Cu content in response to changes in Cu bioavailability. The findings in this study are discussed in the context of implications for seaweed biomonitoring.
authors.affiliation: School of Marine Science and Engineering, Plymouth University, Plymouth, UK. mtbrown@plymouth.ac.uk
authors.forename: Murray T
authors.initials: MT
authors.lastname: Brown
authors.forename: James E
authors.initials: JE
authors.lastname: Newman
authors.forename: Taejun
authors.initials: T
authors.lastname: Han
mesh_terms: D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods; D000461:Rhodophyta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012624:Seaweed / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D003300:Copper
references.citation: Aquat Toxicol. 2003 Jul 16;64(2):201-13
references.pmid: 12799112
references.citation: Aquat Toxicol. 2007 Feb 15;81(1):65-72
references.pmid: 17161878
references.citation: Aquat Toxicol. 2008 Jan 31;86(2):176-84
references.pmid: 18083244
references.citation: Environ Monit Assess. 1996 Mar;40(1):41-54
references.pmid: 24198070
references.citation: Mar Environ Res. 2008 Oct;66(4):438-44
references.pmid: 18760467
references.citation: Mar Pollut Bull. 2004 May;48(9-10):902-9
references.pmid: 15111037
references.citation: Nature. 1970 Oct 17;228(5268):288-9
references.pmid: 5479530
references.citation: Aquat Toxicol. 2007 Jul 20;83(3):190-9
references.pmid: 17532484
references.citation: Biol Rev Camb Philos Soc. 1994 May;69(2):147-86
references.pmid: 8054443
references.citation: Environ Pollut. 2008 Dec;156(3):897-904
references.pmid: 18635296
references.citation: Environ Int. 2001 Jul;27(1):43-7
references.pmid: 11488389
references.citation: Chemosphere. 2003 Feb;50(6):795-800
references.pmid: 12688493
references.citation: Environ Pollut. 1992;76(2):89-131
references.pmid: 15091993
references.citation: Chemosphere. 2010 Jan;78(4):397-401
references.pmid: 19962173
references.citation: Environ Pollut. 1987;45(3):173-205
references.pmid: 15092750
references.citation: Environ Pollut. 2006 May;141(1):69-80
references.pmid: 16214279