title: CD8αα and -αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I.
abstract: Bimolecular fluorescence complementation was used to engineer CD8 molecules so that CD8αα and CD8αβ dimers can be independently visualized on the surface of a T cell during antigen recognition. Using this approach, we show that CD8αα is recruited to the immunological synapse almost as well as CD8αβ, but because the kinase Lck associates preferentially with CD8αβ in lipid rafts, CD8αα is the weaker co-receptor. During recognition of the strong CD8αα ligand H2-TL, CD8αα is preferentially recruited. Thus, recruitment of the two CD8 species correlates with their relative binding to the available ligands, rather than with the co-receptor functions of the CD8 species.
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037-1092, USA.
authors.forename: Vasily
authors.initials: V
authors.lastname: Rybakin
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Clamme
authors.forename: Jeanette
authors.initials: J
authors.lastname: Ampudia
authors.forename: Pia P
authors.initials: PP
authors.lastname: Yachi
authors.forename: Nicholas R J
authors.initials: NR
authors.lastname: Gascoigne
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism*; D005453:Fluorescence; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D054992:Immunological Synapses / Q000276:immunology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; C433839:CD8alphabeta antigen; D015395:Histocompatibility Antigens Class I; D008024:Ligands; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; C028388:thymus-leukemia antigens
references.citation: Nature. 1996 Dec 12;384(6609):577-81
references.pmid: 8955273
references.citation: J Immunol. 2009 Aug 15;183(4):2554-64
references.pmid: 19625641
references.citation: Biophys J. 2004 Jun;86(6):3923-39
references.pmid: 15189889
references.citation: J Immunol. 1992 Sep 1;149(5):1613-8
references.pmid: 1506684
references.citation: Mol Cell Biol. 1994 Aug;14(8):5384-91
references.pmid: 8035816
references.citation: Nature. 1990 Aug 2;346(6283):476-80
references.pmid: 2198471
references.citation: J Immunol. 2005 Mar 15;174(6):3501-7
references.pmid: 15749886
references.citation: J Exp Med. 2007 Oct 29;204(11):2747-57
references.pmid: 17954567
references.citation: Biotechniques. 2006 Jan;40(1):61-6
references.pmid: 16454041
references.citation: Immunity. 2006 Aug;25(2):203-11
references.pmid: 16872849
references.citation: J Exp Med. 1999 Nov 15;190(10):1517-26
references.pmid: 10562325
references.citation: Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16916-21
references.pmid: 20837541
references.citation: Nat Immunol. 2005 Aug;6(8):785-92
references.pmid: 15980863
references.citation: Immunity. 2008 Feb;28(2):149-59
references.pmid: 18275828
references.citation: Science. 2001 Nov 30;294(5548):1936-9
references.pmid: 11729321
references.citation: J Immunol. 2002 Nov 15;169(10):5708-14
references.pmid: 12421950
references.citation: J Immunol. 2002 Apr 15;168(8):3881-6
references.pmid: 11937542
references.citation: Proc Natl Acad Sci U S A. 1989 May;86(9):3277-81
references.pmid: 2470098
references.citation: J Exp Med. 2001 Nov 19;194(10):1485-95
references.pmid: 11714755
references.citation: Nat Biotechnol. 2003 May;21(5):539-45
references.pmid: 12692560
references.citation: J Biol Chem. 2003 Jan 31;278(5):3257-64
references.pmid: 12215456
references.citation: J Biol Chem. 1999 Sep 17;274(38):27237-43
references.pmid: 10480942
references.citation: J Immunol. 2000 Aug 15;165(4):2068-76
references.pmid: 10925291
references.citation: J Cell Biol. 2002 Sep 30;158(7):1263-75
references.pmid: 12356870
references.citation: J Immunol. 1987 Dec 1;139(11):3585-9
references.pmid: 2960738
references.citation: J Exp Med. 1996 Dec 1;184(6):2439-44
references.pmid: 8976201
references.citation: Curr Opin Immunol. 1998 Feb;10(1):82-7
references.pmid: 9523116
references.citation: EMBO J. 1998 Sep 15;17(18):5334-48
references.pmid: 9736612
references.citation: Cell. 1988 Oct 21;55(2):301-8
references.pmid: 3262426
references.citation: Immunity. 2003 Feb;18(2):205-15
references.pmid: 12594948
references.citation: Mol Cell. 2002 Apr;9(4):789-98
references.pmid: 11983170
references.citation: Curr Top Microbiol Immunol. 2010;340:171-89
references.pmid: 19960314
references.citation: J Immunol. 2006 Sep 15;177(6):3930-8
references.pmid: 16951356