title: Induced over-expression of the transcription factor OsDREB2A improves drought tolerance in rice.
abstract: The DREB and CBF transcription factors play a critical role in plant development and abiotic stress responses and, therefore, represent attractive targets for a molecular plant breeding approach. In this study, the rice OsDREB2A gene was isolated and expressed under the control of a stress-inducible promoter (4ABRC) to improve the abiotic stress tolerance of japonica rice variety TNG67. T₂ and T₃ transgenic lines over-expressing OsDREB2A were found to have improved survival rates under severe drought and salt stress conditions relative to non-transgenic rice plants or rice plants transformed with the empty vector control. OsDREB2A expression was found to be markedly induced by drought and ABA treatment. The results indicate that the induced over-expression of OsDREB2A driven by the 4ABRC promoter in engineered rice plants may protect cells during stress.
authors.affiliation: Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Cui
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xu
authors.forename: Zhengge
authors.initials: Z
authors.lastname: Zhu
authors.forename: Faping
authors.initials: F
authors.lastname: Duan
authors.forename: Ray
authors.initials: R
authors.lastname: Wu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D005818:Genetic Engineering; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D055049:Salt Tolerance / Q000235:genetics*; D012492:Salts / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C114579:DREB2A protein, Arabidopsis; D010937:Plant Growth Regulators; D012492:Salts; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid