title: Downregulation of EphA2 expression suppresses the growth and metastasis in squamous-cell carcinoma of the head and neck in vitro and in vivo.
abstract: OBJECTIVE
  Our previous study has revealed that EphA2 overexpression is significantly associated with aggressive behavior and poor prognosis in patients with squamous-cell carcinoma of the head and neck (SCCHN). However, the function of EphA2 in tumorigenesis and cervical lymph node metastasis of SCCHN has never been elucidated in vivo.
  
  
  METHODS
  EphA2 was knocked down in SCCHN cell lines. CCK-8 assays, fluorescence-activated cell sorting analysis, invasion and migration assays were performed in vitro. In vivo tumorigenicity assays were performed, and the impact on cervical lymph node metastasis was evaluated.
  
  
  RESULTS
  The present investigation demonstrated that suppression of EphA2 resulted in a significant inhibition of proliferation, migration, invasion of SCCHN cells in vitro and markedly diminished their tumorigenicity and lymph node metastasis in vivo.
  
  
  CONCLUSIONS
  These results suggest that EphA2 plays a critical role in SCCHN growth and metastasis and may be a promising therapeutic target to prevent the progression of SCCHN.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha, 410008, Hunan Province, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Changyun
authors.initials: C
authors.lastname: Yu
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Qiu
authors.forename: Donghai
authors.initials: D
authors.lastname: Huang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016193:G1 Phase / Q000235:genetics; D055785:Gene Knockdown Techniques / Q000379:methods; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D036104:Receptor, EphA2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics*; D016192:Resting Phase, Cell Cycle / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D034741:RNA, Small Interfering; D036104:Receptor, EphA2
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