title: [Cloning, expression, isolation and properties of thymidine kinase herpes simplex virus, strain L2].
abstract: A thymidine kinase UL23 gene (EC 2.7.1.145) from an acyclovir-sensitive strain L2 of herpes simplex virus type 1 was cloned and expressed in E. coli. Enzyme was purified by chromatography to a homogeneous state controlled by PAG electrophoresis. The Michaelis constants for the reactions with thymidine and an acyclovir were determined. It was found that enzyme phosphorilate some modified nucleosides such as d2T, d4T, d2C, 3TC, FLT, BVDU, GCV. A comparison of the purified enzyme properties and properties ofthymidine kinase of other strains of herpes simplex virus, previously published was carried out. It is shown that enzyme is inhibited by acyclovir H-phosphonate.
authors.forename: V N
authors.initials: VN
authors.lastname: Stepanenko
authors.forename: R  S
authors.initials: R
authors.lastname: Esipov
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
authors.forename: V L
authors.initials: VL
authors.lastname: Andronova
authors.forename: G A
authors.initials: GA
authors.lastname: Galegov
authors.forename: M V
authors.initials: MV
authors.lastname: Ias'ko
authors.forename: A V
authors.initials: AV
authors.lastname: Gus'kova
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Skoblov
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Skoblov
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D013937:Thymidine Kinase; D000212:Acyclovir