title: Effect of mosquito mats (pyrethroid-based) vapor inhalation on rat brain cytochrome P450s.
abstract: The effect of transfluthrin (TF) or D-allethrin (DA) pyrethroid (PYR) vapors, often contained as main ingredients in two commercially available mosquito repellent mats, on cytochrome P450 (CYP) enzymes of rat brain and liver was assessed. Immunodetection of CYP2E1 and CYP3A2 proteins revealed their induction in cerebrum and cerebellum, but not in liver microsomes of rats exposed by inhalation to TF or DA. This overexpression of proteins correlated with an increase of their catalytic activities. The specifically increased expression of CYP isoenzymes, due to PYR exposure in the rat brain, could perturb the normal metabolism of endogenous and xenobiotic compounds and leads to increased risks of neurotoxicity by bioactivation, lipid peroxidation and DNA damage.
authors.affiliation: Laboratorio de Toxicología Genética, Instituto Nacional de Pediatría (INP), México DF, México. spolak@cm-uj.krakow.pl
authors.forename: Araceli
authors.initials: A
authors.lastname: Vences-Mejía
authors.forename: Josefina
authors.initials: J
authors.lastname: Gómez-Garduño
authors.forename: Heriberto
authors.initials: H
authors.lastname: Caballero-Ortega
authors.forename: Víctor
authors.initials: V
authors.lastname: Dorado-González
authors.forename: Rosario
authors.initials: R
authors.lastname: Nosti-Palacios
authors.forename: Norma
authors.initials: N
authors.lastname: Labra-Ruíz
authors.forename: J Javier
authors.initials: JJ
authors.lastname: Espinosa-Aguirre
mesh_terms: D000487:Allethrins / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D003521:Cyclopropanes / Q000737:chemistry / Q000633:toxicity; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005464:Fluorobenzenes / Q000737:chemistry / Q000633:toxicity; D019570:Inhalation Exposure; D057148:Insecticide-Treated Bednets / Q000009:adverse effects; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D020258:Neurotoxicity Syndromes / Q000201:enzymology / Q000209:etiology; D011722:Pyrethrins / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014835:Volatilization
chemical_list: D000487:Allethrins; D003521:Cyclopropanes; D005464:Fluorobenzenes; D007306:Insecticides; D008565:Membrane Proteins; D011722:Pyrethrins; D003577:Cytochrome P-450 Enzyme System; D019392:Cytochrome P-450 CYP2E1; C465260:Cyp3a2 protein, rat; D051544:Cytochrome P-450 CYP3A; C560613:transfluthrin