title: Pyranocoumarins isolated from Peucedanum praeruptorum Dunn suppress lipopolysaccharide-induced inflammatory response in murine macrophages through inhibition of NF-κB and STAT3 activation.
abstract: Praeruptorin C, D, and E (PC, PD, and PE) are three pyranocoumarins isolated from the dried root of Peucedanum praeruptorum Dunn of Umbelliferae. In the present study, we investigated the anti-inflammatory effect of these compounds in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Pyranocoumarins significantly inhibited LPS-induced production of nitric oxide, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). The mRNA and protein expressions of inducible nitric oxide synthase, IL-6, and TNF-α were also suppressed by these compounds. Both PD and PE exhibited greater anti-inflammatory activities than PC. Further study showed that pyranocoumarins suppressed the cytoplasmic loss of inhibitor κB-α protein and inhibited the translocation of NF-κB from cytoplasm to nucleus. In addition, pyranocoumarins suppressed LPS-induced STAT3 tyrosine phosphorylation. Taken together, the results suggest that pyranocoumarins may exert anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages through the inhibition of NF-κB and STAT3 activation.
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
authors.forename: Peng-Jiu
authors.initials: PJ
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Zhang
authors.forename: Guang-Fa
authors.initials: GF
authors.lastname: Wang
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Li
authors.forename: Zheng-Guang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Yuan-Xin
authors.initials: YX
authors.lastname: Tian
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Jia-Jie
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Wu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D019661:Apiaceae; D002460:Cell Line; D003374:Coumarins / Q000494:pharmacology; D051550:I-kappa B Kinase / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy*; D018836:Inflammation Mediators; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D039681:Pyranocoumarins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D039681:Pyranocoumarins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; C036455:praeruptorin E; C058808:praeruptorin C; D052247:Nitric Oxide Synthase Type II; D051550:I-kappa B Kinase
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