title: Antinociception of β-D-glucan from Pleurotus pulmonarius is possibly related to protein kinase C inhibition.
abstract: β-D-Glucan, a polysaccharide isolated from an edible mushroom Pleurotus pulmonarius (Fr.) Quel., presented antinociceptive activity in mice. This study evaluated the involvement of transient receptor potential (TRP) channels and protein kinase C (PKC) on antinociceptive effect of a (1→3),(1→6)-linked β-D-glucan (GL) in mice. Intraperitoneal administration of GL potently inhibited nociceptive responses induced by intraplantar injections of capsaicin, cinnamaldehyde, menthol, acidified saline and phorbol myristate acetate (PMA). Moreover, Western blot analysis revealed that GL treatment also prevented PMA-induced PKCɛ activation. Collectively, present results demonstrate that GL could constitute an attractive molecule of interest for the development of new analgesic drugs.
authors.affiliation: Department of Pharmacology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Cristina Setim
authors.initials: CS
authors.lastname: Freitas
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Marcon
authors.forename: Maria Fernanda de Paula
authors.initials: MF
authors.lastname: Werner
authors.forename: Giles Alexander
authors.initials: GA
authors.lastname: Rae
authors.forename: Fhernanda Ribeiro
authors.initials: FR
authors.lastname: Smiderle
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Maria Consuelo Andrade
authors.initials: MC
authors.lastname: Marques
authors.forename: Adair Roberto Soares
authors.initials: AR
authors.lastname: Santos
mesh_terms: D062565:Acid Sensing Ion Channels; D000171:Acrolein / Q000031:analogs & derivatives / Q000494:pharmacology; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005936:Glucans / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008610:Menthol / Q000494:pharmacology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D059225:Nociception / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D015222:Sodium Channels / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D062565:Acid Sensing Ion Channels; D000700:Analgesics; D005936:Glucans; D009419:Nerve Tissue Proteins; D047428:Protein Kinase Inhibitors; D015222:Sodium Channels; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D008610:Menthol; D000171:Acrolein; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002211:Capsaicin; C012843:cinnamaldehyde