title: Dopamine control of LH release in the tench (Tinca tinca).
abstract: Tench (Tinca tinca) is apparently the only known member of the Cyprinidae in which ovulation is stimulated following administration of a low dose of GnRH analogue (GnRHa) without a dopamine inhibitor. This study evaluated LH release effectiveness of the most commonly used GnRHa and clarified whether LH secretion followed by ovulation is subject to inhibitory dopaminergic control in tench. Fish were intraperitoneally injected with three types of GnRHa, GnRHa with dopamine inhibitor metoclopramide (combined treatment), or the dopamine inhibitor metoclopramide alone. LH concentrations at five sampling times (0, 6, 12, 24, and 33 h) together with ovulation success and fecundity index were recorded. The combined treatment triggered an almost immediate LH release peak with a gradual decline, and resulted in a high ovulation rate. In contrast to the combined treatment, an application of GnRHa alone at 10 μg kg(-1) induced gradual increase of LH concentrations with peaks close to ovulation time, and with high ovulation success. Significant differences in LH concentrations at 6 and 12h and no differences in ovulation success were found between the combined and the GnRHa alone treatments. Metoclopramide alone induced a small increase in LH with no ovulation. The study presents clear evidence of dopaminergic control of LH release in tench, with a high ovulation rate obtained after application of GnRHa alone or in combination with dopamine inhibitor.
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic. podhop01@vurh.jcu.cz
authors.forename: Peter
authors.initials: P
authors.lastname: Podhorec
authors.forename: Magdalena
authors.initials: M
authors.lastname: Socha
authors.forename: Miroslawa
authors.initials: M
authors.lastname: Sokolowska-Mikolajczyk
authors.forename: Tomas
authors.initials: T
authors.lastname: Policar
authors.forename: Viktor W
authors.initials: VW
authors.lastname: Svinger
authors.forename: Borek
authors.initials: B
authors.lastname: Drozd
authors.forename: Jan
authors.initials: J
authors.lastname: Kouril
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D004298:Dopamine / Q000502:physiology*; D018492:Dopamine Antagonists / Q000494:pharmacology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000502:physiology*; D008787:Metoclopramide / Q000494:pharmacology; D010060:Ovulation / Q000187:drug effects / Q000502:physiology
chemical_list: D018492:Dopamine Antagonists; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D008787:Metoclopramide; D004298:Dopamine