title: Mechanism of structural transformations induced by antimicrobial peptides in lipid membranes.
abstract: It has long been suggested that pore formation is responsible for the increase in membrane permeability by antimicrobial peptides (AMPs). To better understand the mechanism of AMP activity, the disruption of model membrane by protegrin-1 (PG-1), a cationic antimicrobial peptide, was studied using atomic force microscopy. We present here the direct visualization of the full range of structural transformations in supported lipid bilayer patches induced by PG-1 on zwitterionic 1,2-dimyristoyl-snglycero-phospho-choline (DMPC) membranes. When PG-1 is added to DMPC, the peptide first induces edge instability at low concentrations, then pore-like surface defects at intermediate concentrations, and finally wormlike structures with a specific length scale at high concentrations. The formation of these structures can be understood using a mesophase framework of a binary mixture of lipids and peptides, where PG-1 acts as a line-active agent. Atomistic molecular dynamics simulations on lipid bilayer ribbons with PG-1 molecules placed at the edge or interior positions are carried out to calculate the effect of PG-1 in reducing line tension. Further investigation of the placement of PG-1 and its association with defects in the bilayer is carried out using unbiased assembly of a PG-1 containing bilayer from a random mixture of PG-1, DMPC, and water. A generalized model of AMP induced structural transformations is also presented in this work. This article is part of a Special Issue entitled: Membrane protein structure and function.
authors.affiliation: Department of Physics, The University of Chicago, Chicago, IL, USA.
authors.forename: Kin Lok H
authors.initials: KL
authors.lastname: Lam
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Ting Ann
authors.initials: TA
authors.lastname: Siaw
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Chapman
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Waring
authors.forename: James T
authors.initials: JT
authors.lastname: Kindt
authors.forename: Ka Yee C
authors.initials: KY
authors.lastname: Lee
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017433:Protein Structure, Secondary
chemical_list: D023181:Antimicrobial Cationic Peptides; D008563:Membrane Lipids; C094880:protegrin-1; D004134:Dimyristoylphosphatidylcholine