title: Effect of ether- and water-soluble fractions of Carica papaya ethanol extract in experimentally induced hyperlipidemia in rats.
abstract: BACKGROUND
  The papaya is the fruit of the plant Carica papaya L. (Caricaceae) used in India. Fruit and latex are both rich in an enzyme called papain. It is used as a folk remedy for contraception and abortion.
  
  
  OBJECTIVE
  The present study explored the anti-hyperlipidemic effect of the ether- and water-soluble fractions of C. papaya ethanol extract in olive oil-induced hyperlipidemic rats. The study also involved chromatographic studies of extract and fractions.
  
  
  METHODS
  Flash chromatography was done for the most active fraction. The extract and fractions were administered orally at doses of 200 and 400 mg/kg body weight in rats. Olive oil (5 mL/kg oral dose) was administered 30 min after treatment. Blood was collected and centrifuged at 3000 rpm for 15-20 min and subjected to biochemical analysis.
  
  
  RESULTS
  The study dose-dependently inhibited the total cholesterol (TC), triglycerides (TG), low-density lipoproteins (LDL) level, and significantly increased high-density lipoprotein (HDL) level. Phytochemical screening revealed the presence of fats in the ether fraction, whereas the water fraction revealed the presence of tannins, alkaloids, glycosides. UV λ(max) was found to be 217 nm with a melting point of 41°C for the isolated component.
  
  
  CONCLUSIONS
  The anti-hyperlipidemic effect was evaluated in olive oil-loaded rats. Acute treatment caused stimulatory effect on HDL level and inhibition in TC and TG elevation induced by olive oil. The extract and water fraction showed protective action by increasing the HDL cholesterol level.
authors.affiliation: VNS Institute of Pharmacy, Neelbud, Bhopal, Madhya Pradesh, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Iyer
authors.forename: B K
authors.initials: BK
authors.lastname: Sharma
authors.forename: U K
authors.initials: UK
authors.lastname: Patil
mesh_terms: D000818:Animals; D000069059:Atorvastatin; D029441:Carica / Q000737:chemistry*; D008076:Cholesterol, HDL / Q000097:blood; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005260:Female; D006538:Heptanoic Acids / Q000627:therapeutic use; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000627:therapeutic use; D006949:Hyperlipidemias / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000960:Hypolipidemic Agents / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D000069463:Olive Oil; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use; D010938:Plant Oils; D011758:Pyrroles / Q000627:therapeutic use; D051381:Rats; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D008076:Cholesterol, HDL; D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D000960:Hypolipidemic Agents; D000069463:Olive Oil; D010936:Plant Extracts; D010938:Plant Oils; D011758:Pyrroles; D012997:Solvents; D014867:Water; D000431:Ethanol; D000069059:Atorvastatin