title: Organization of the capsule biosynthesis gene locus of the oral streptococcus Streptococcus anginosus.
abstract: The capsular polysaccharide (CPS) of the important oral streptococcus Streptococcus anginosus, which causes endocarditis, and the genes for its synthesis have not been clarified. In this study, we investigated the gene locus required for CPS synthesis in S. anginosus. Southern hybridization using the cpsE gene of the well-characterized bacterium S. agalactiae revealed that there is a similar gene in the genome of S. anginosus. By using the colony hybridization technique and inverse PCR, we isolated the CPS synthesis (cps) genes of S. anginosus. This gene cluster consisted of genes containing typical regulatory genes, cpsA-D, and glycosyltransferase genes coding for glucose, rhamnose, N-acetylgalactosamine, and galactofuranose transferases. Furthermore, we confirmed that the cps locus is required for CPS synthesis using a mutant strain with a defective cpsE gene. The cps cluster was found to be located downstream the nrdG gene, which encodes ribonucleoside triphosphate reductase activator, as is the case in other oral streptococci such as S. gordonii and S. sanguinis. However, the location of the gene cluster was different from those of S. pneumonia and S. agalactiae.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsunashima
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Miyake
authors.forename: Makoto
authors.initials: M
authors.lastname: Motono
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D016667:Bacterial Capsules / Q000235:genetics* / Q000378:metabolism*; D023061:Gene Order; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D013292:Streptococcus agalactiae / Q000235:genetics; D034366:Streptococcus anginosus / Q000235:genetics*