title: New Iranian and Australian peach latent mosaic viroid variants and evidence for rapid sequence evolution.
abstract: Peach latent mosaic viroid isolates from peach and plum in Iran have been compared with an Australian isolate from nectarine. Thirteen sequence variants 336-338 nt in size were obtained. All variants clustered phylogenetically with variants reported from several hosts and countries. A total nucleic acid extract, a slightly longer than full-length RT-PCR amplicon, and a recombinant plasmid clone from the Australian isolate were all infectious to, and symptomatic in, mechanically inoculated peach seedlings. The infectious clone generated two progeny viroid molecules, which each showed 10 different mutations compared with the parent clone inoculated 30 days previously.
authors.affiliation: Department of Plant Pathology, Ferdowsi University of Mashhad, Mashhad, Iran. arezou.yazarlou@adelaide.edu.au
authors.forename: Arezou
authors.initials: A
authors.lastname: Yazarlou
authors.forename: Behrooz
authors.initials: B
authors.lastname: Jafarpour
authors.forename: Saeed
authors.initials: S
authors.lastname: Tarighi
authors.forename: Nuredin
authors.initials: N
authors.lastname: Habili
authors.forename: John W
authors.initials: JW
authors.lastname: Randles
mesh_terms: D001315:Australia; D001483:Base Sequence; D019143:Evolution, Molecular; D014644:Genetic Variation; D007492:Iran; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D027861:Prunus / Q000821:virology*; D014772:Viroids / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*