title: Escherichia coli SlyD, more than a Ni(II) reservoir.
abstract: SlyD interacts with HypB and contributes to nickel insertion during [NiFe]-hydrogenase biogenesis. Herein, we provide evidence of SlyD acting as a nickel storage determinant in Escherichia coli and show that this Ni(II) can be mobilized to HypB in vitro even under competitive conditions. Furthermore, SlyD enhances the GTPase activity of HypB, and acceleration of release of Ni(II) from HypB is more pronounced when HypB is GDP-bound. The data support a model in which a HypB-SlyD complex establishes communication between GTP hydrolysis and nickel delivery and provide insight into the role of the HypB-SlyD complex during [NiFe]-hydrogenase biosynthesis.
authors.affiliation: Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3H6.
authors.forename: Harini
authors.initials: H
authors.lastname: Kaluarachchi
authors.forename: Jei Wei
authors.initials: JW
authors.lastname: Zhang
authors.forename: Deborah B
authors.initials: DB
authors.lastname: Zamble
mesh_terms: D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D055162:Biocatalysis; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006864:Hydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008956:Models, Chemical; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D009532:Nickel / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001059:Apoproteins; D002352:Carrier Proteins; D002614:Chelating Agents; D029968:Escherichia coli Proteins; C490324:HypB protein, E coli; D050505:Mutant Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C085606:SlyD protein, E coli; D006153:Guanosine Diphosphate; D009532:Nickel; D006160:Guanosine Triphosphate; C043296:nickel-iron hydrogenase; D006864:Hydrogenase; D019204:GTP-Binding Proteins; D019696:Peptidylprolyl Isomerase
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