title: Generation of continuous packed bed reactor with PVA-alginate blend immobilized Ochrobactrum sp. DGVK1 cells for effective removal of N,N-dimethylformamide from industrial effluents.
abstract: Effective removal of dimethylformamide (DMF), the organic solvent found in industrial effluents of textile and pharma industries, was demonstrated by using free and immobilized cells of Ochrobactrum sp. DGVK1, a soil isolate capable of utilizing DMF as a sole source of carbon, nitrogen. The free cells have efficiently removed DMF from culture media and effluents, only when DMF concentration was less than 1% (v/v). Entrapment of cells either in alginate or in polyvinyl alcohol (PVA) failed to increase tolerance limits. However, the cells of Ochrobactrum sp. DGVK1 entrapped in PVA-alginate mixed matrix tolerated higher concentration of DMF (2.5%, v/v) and effectively removed DMF from industrial effluents. As determined through batch fermentation, these immobilized cells have retained viability and degradability for more than 20 cycles. A continuous packed bed reactor, generated by using PVA-alginate beads, efficiently removed DMF from industrial effluents, even in the presence of certain organic solvents frequently found in effluents along with DMF.
authors.affiliation: Department of Biochemistry, Gulbarga University, Gulbarga 585106, Karnataka, India.
authors.forename: S Sanjeev
authors.initials: SS
authors.lastname: Kumar
authors.forename: M Santosh
authors.initials: MS
authors.lastname: Kumar
authors.forename: D
authors.initials: D
authors.lastname: Siddavattam
authors.forename: T B
authors.initials: TB
authors.lastname: Karegoudar
mesh_terms: D000464:Alginates / Q000737:chemistry*; D004126:Dimethylformamide; D005285:Fermentation; D005559:Formamides / Q000302:isolation & purification*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007220:Industrial Waste; D008855:Microscopy, Electron, Scanning; D042041:Ochrobactrum / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D000464:Alginates; D005559:Formamides; D006603:Hexuronic Acids; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D004126:Dimethylformamide; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol