title: AXY8 encodes an α-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides.
abstract: An Arabidopsis thaliana mutant with an altered structure of its hemicellulose xyloglucan (XyG; axy-8) identified by a forward genetic screen facilitating oligosaccharide mass profiling was characterized. axy8 exhibits increased XyG fucosylation and the occurrence of XyG fragments not present in the wild-type plant. AXY8 was identified to encode an α-fucosidase acting on XyG that was previously designated FUC95A. Green fluorescent protein fusion localization studies and analysis of nascent XyG in microsomal preparations demonstrated that this glycosylhydrolase acts mainly on XyG in the apoplast. Detailed structural analysis of XyG in axy8 gave unique insights into the role of the fucosidase in XyG metabolism in vivo. The genetic evidence indicates that the activity of glycosylhydrolases in the apoplast plays a major role in generating the heterogeneity of XyG side chains in the wall. Furthermore, without the dominant apoplastic glycosylhydrolases, the XyG structure in the wall is mainly composed of XXXG and XXFG subunits.
authors.affiliation: University of California, Berkeley, California, 94720, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Günl
authors.forename: Lutz
authors.initials: L
authors.lastname: Neumetzler
authors.forename: Florian
authors.initials: F
authors.lastname: Kraemer
authors.forename: Amancio
authors.initials: A
authors.lastname: de Souza
authors.forename: Alex
authors.initials: A
authors.lastname: Schultink
authors.forename: Maria
authors.initials: M
authors.lastname: Pena
authors.forename: William S
authors.initials: WS
authors.lastname: York
authors.forename: Markus
authors.initials: M
authors.lastname: Pauly
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D014990:Xylans / Q000737:chemistry / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; C007916:hemicellulose; D005644:alpha-L-Fucosidase; C574397:alpha-L-fucosidase 2, Arabidopsis
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