title: [Genotyping for CYP2C19 with multiplex PCR point mutation screening technology].
abstract: OBJECTIVE
  To establish a multiplex PCR point mutation screening technique for the genotyping of CYP2C19.
  
  
  METHODS
  Deoxyinosine multiplex-polymerase chain reaction (PCR) primers (DMPs) were designed to detect simultaneously CYP2C19*1,*2,*3 alleles in one PCR tube.
  
  
  RESULTS
  The above technique could detect the genotypes of CYP2C19*1, CYP2C19*2 and CYP2C19*3 successfully. And the results were completely consistent with those of DNA sequencing.
  
  
  CONCLUSIONS
  A novel screening technique of multiplex PCR point mutation is successfully established. With the advantages of high specificity, convenient handling, fast completion and low cost, it provides a reasonable and reliable detection method for basic researches and personalized medicine.
authors.affiliation: Department of Biochemistry, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.forename: Jia-chang
authors.initials: JC
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Peng
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Hua-yun
authors.initials: HY
authors.lastname: Chen
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D065731:Cytochrome P-450 CYP2C19; D017931:DNA Primers; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017354:Point Mutation
chemical_list: D017931:DNA Primers; D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19