title: Differential expression of FCRLA in naïve and activated mouse B cells.
abstract: FCRLA is an intracellular B cell protein that belongs to the FcR-like family. Using newly generated FCRLA-specific antibodies, we studied the constitutive expression pattern of mouse FCRLA and monitored changes during an immune response and following in vitro B cell activation. All B cell subpopulations examined expressed FCRLA. However, the level of FCRLA expression is determined by the stage of B cell differentiation. Low expression of FCRLA is characteristic of naïve follicular and marginal zone B cells. High expression was detected in a small fraction of activated B cells scattered along migratory pathways in the lymphoid tissues. FCRLA-bright cells could be subdivided into two subpopulations, with high and low/undetectable level of intracellular immunoglobulins, which phenotypically resemble either plasma or memory B cells. High expression of FCRLA in subset(s) of terminally differentiated B-cells suggests that, being an ER protein, FCRLA may participate in the regulation of immunoglobulin assembly and secretion.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
authors.forename: Evdokiya S
authors.initials: ES
authors.lastname: Reshetnikova
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Mechetina
authors.forename: Olga Y
authors.initials: OY
authors.lastname: Volkova
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Guselnikov
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Chikaev
authors.forename: Dorottya
authors.initials: D
authors.lastname: Kövesdi
authors.forename: Boris
authors.initials: B
authors.lastname: Alabyev
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sármay
authors.forename: Peter D
authors.initials: PD
authors.lastname: Burrows
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Najakshin
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Taranin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D001853:Bone Marrow / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D005260:Female; D007136:Immunoglobulins / Q000276:immunology / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D000906:Antibodies; C524782:Fcrla protein, mouse; D007136:Immunoglobulins; D047908:Intracellular Signaling Peptides and Proteins; D011971:Receptors, Immunologic
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