title: Messenger RNA expression and genetic polymorphisms of cell cycle control genes and chromosomal aberrations in Chinese vinyl chloride monomer-exposed workers.
abstract: OBJECTIVE
  To evaluate the expressions of p53, p21, and CCND1 in the peripheral blood lymphocytes of vinyl chloride monomer (VCM)-exposed workers and potential relationships with their exposures, polymorphisms, and chromosomal aberrations.
  
  
  METHODS
  The study was performed on 77 occupationally VCM-exposed workers and 43 unexposed controls. The quantities of mRNA expression of p53, p21, and CCND1 genes were detected by real-time polymerase chain reaction.
  
  
  RESULTS
  p53 mRNA expression of VCM-low- and high-exposure groups was significantly lower than that of nonexposed group (P < 0.001), but p21 mRNA expression of the two VCM-exposed groups was significantly higher than that of the nonexposed group (P < 0.001). This study did not find the relationship between chromosomal aberrations, genotypes, and the expression of p53, p21, and CCND1.
  
  
  CONCLUSIONS
  Messenger RNA expressions of p53 and p21 are changed with VCM-exposure status.
authors.affiliation: Department of Health Toxicology, Box 80, School of Public Health, Shanxi Medical University, Taiyuan 030001, China. ylqiu@sina.com
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Qiu
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Qiu
authors.forename: Pin
authors.initials: P
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.forename: Zhao-Lin
authors.initials: ZL
authors.lastname: Xia
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D002869:Chromosome Aberrations; D019938:Cyclin D1 / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D014752:Vinyl Chloride / Q000633:toxicity*
chemical_list: C530104:CCND1 protein, human; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D016283:Proto-Oncogene Proteins p21(ras); D014752:Vinyl Chloride