title: Role of hypoxia-inducible-1α in hepatocellular carcinoma cells using             a Tet-on inducible system to regulate its expression in vitro.
abstract: Hypoxia-inducible-1α (HIF-1α) expression was intimately correlated with             apoptosis and proliferation of cancer cells. However, conclusions of different             studies on the effects of HIF-1α expression on cell apoptosis and cell proliferation             of hepatoma cells remain controversial. In view of the current status, we reassess             its roles and possible mechanism in hepatoma cells. In order to acquire more convincing             and reliable results, we used a Tet-on system to stably and effectively regulate             HIF-1α expression in the HepG2 cells in vitro. In our study we not only confirmed             some common conclusions of previous studies, but also acquired some different             and significant results that HIF-1α facilitates cell proliferation and cell cycle             through influencing the expression of cyclin A and cyclin D, and suppresses cell             apoptosis through inducing the expression of survivin and Bcl-2. These results             further enrich our knowledge on the role of HIF-1α expression on cell apoptosis             and cell proliferation of hepatoma cells.
authors.affiliation: Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan 250012, PR China.
authors.forename: Zongquan
authors.initials: Z
authors.lastname: Xu
authors.forename: Enyu
authors.initials: E
authors.lastname: Liu
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Yunguang
authors.initials: Y
authors.lastname: Li
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: He
authors.forename: Chuanzong
authors.initials: C
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Niu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019925:Cyclin A / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D019254:Genes, bcl-2; D056945:Hep G2 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D019925:Cyclin A; D019927:Cyclin E; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D019938:Cyclin D1; D053148:Caspase 3; D004318:Doxycycline