title: Polyclonal diffusion of beta-lactamase-producing Enterococcus faecium.
abstract: We describe here the isolation of 8 beta-lactamase-producing multidrug-resistant Enterococcus faecium isolates in 2010. All strains showed diverse pulsed-field gel electrophoresis (PFGE) profiles, all belonging to the same clonal complex, CC17. By PCR and hybridization experiments, the entire blaZ-blaI-blaR1 operon was found. The beta-lactamase activity was demonstrated at a high inoculum and in the presence of methicillin after overnight incubation.
authors.affiliation: Clinical Microbiology, S. Agostino-Estense Hospital, Baggiovara, Modena, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Sarti
authors.forename: Floriana
authors.initials: F
authors.lastname: Campanile
authors.forename: Carla
authors.initials: C
authors.lastname: Sabia
authors.forename: Maria
authors.initials: M
authors.lastname: Santagati
authors.forename: Raffaele
authors.initials: R
authors.lastname: Gargiulo
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000145:classification* / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D058889:Molecular Typing; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases; D008712:Methicillin
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