title: [Technical strategies in the research of plant salt-responsive proteomics: a review].
abstract: Soil salinization is one of the key factors limiting plant growth and distribution. To explore plant molecular salt-responsive mechanisms is the basis of enhancing plant salt tolerance in virtue of molecular biological techniques, and also, the hotspot of plant physiology and molecular ecology. High throughput proteomics approaches include two-dimensional electrophoresis (2-DE), blue-native/SDS-PAGE (BN/SDS-PAGE), two-dimensional fluorescence difference gel electrophoresis (2D DIGE), liquid chromatography (LC), and various mass spectrometry (MS) techniques, being widely applied in the research of plant salt-response and supplied important information for understanding plant molecular salt-tolerant mechanisms. In this paper, a review was made on the technical strategies applied in the research of plant salt-responsive proteomics.
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China. hrb-zhang@hotmail.com
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Dai
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D054712:Salinity; D055049:Salt Tolerance; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins