title: Synthesis and characterization of CLEA-lysozyme immobilized PS/PSMA nanofiber.
abstract: Using 0.2% glutaraldehyde as the cross-linker, lysozyme was covalently immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The lysozyme capacity of PS/PSMA nanofibers under optimal condition was 57.6 mg/g of nanofibers. Various parameters were used to evaluate the stability of the immobilized CLEA-lysozyme. Compared to free enzyme, the immobilized CLEA-lysozyme exhibited its optimal enzymatic activity at higher temperature and pH. The immobilized CLEA-lysozyme maintained more than 78% of its initial activity during 30 days of storage period. Additionally, the immobilized CLEA-lysozyme presented a high antibacterial activity against Staphylococcus aureus. The durability determinations of such nanofibers showed 90.3% retention of the initial lysozyme activity after 80 consecutive reuses, and 81.2% of bacteriostasis ratio after 10 cycles. The results of this study suggest that CLEA-lysozyme immobilized nanofiber which can stabilize its enzymatic activity through cross-linking immobilization can be beneficial for various antibacterial processes.
authors.affiliation: Departmet of Microbiology, Chungbuk National University 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Kim
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Jihee
authors.initials: J
authors.lastname: Yoon
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D004800:Enzymes, Immobilized / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008298:Maleates / Q000737:chemistry* / Q000494:pharmacology; D009113:Muramidase / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D057139:Nanofibers; D011137:Polystyrenes / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D008298:Maleates; D011137:Polystyrenes; C521900:poly(styrene-co-maleic anhydride); D009113:Muramidase