title: Fatty acids and gene expression responses to bioactive compounds in Atlantic salmon (Salmo salar L.) hepatocytes.
abstract: OBJECTIVE
  Effects of bioactive compounds on expression of genes involved in lipid metabolism and fatty acid composition were investigated in Atlantic salmon (Salmo salar L.) primary hepatocytes.
  
  
  METHODS
  Five treatments were investigated: I) genistein 0.005 mM, II) genistein 0.025 mM, III) lipoic acid 0.2 mM, IV) sesamin/episesamin 0.05 mM, V) sesamin 0.05 mM and compared to controls. The relative expression of genes involved in lipid homeostasis was analysed after 12h and 48h.
  
  
  RESULTS
  Incubation with lipoic acid, sesamin and episesamin/sesamin for 48h had significant effect on all analysed genes involved in lipid uptake, β-oxidation, elongation and desaturation, some effects were detected on the expression of peroxisome prolifertor-activated receptor (PPARs). Also effects on the fatty acid composition were found.
  
  
  CONCLUSIONS
  The strongest effect of bioactive copounds on hepatocyte gene expression was detected after 48 hours.
authors.affiliation: Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Anna Lotta
authors.initials: AL
authors.lastname: Schiller Vestergren
authors.forename: Sofia
authors.initials: S
authors.lastname: Trattner
authors.forename: Jan
authors.initials: J
authors.lastname: Mráz
authors.forename: Bente
authors.initials: B
authors.lastname: Ruyter
authors.forename: Jana
authors.initials: J
authors.lastname: Pickova
mesh_terms: D000818:Animals; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004149:Dioxoles / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D017705:Lignans / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020317:Salmo salar / Q000235:genetics* / Q000378:metabolism*; D008063:Thioctic Acid / Q000494:pharmacology
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D004149:Dioxoles; D005227:Fatty Acids; D007202:Indicators and Reagents; D017705:Lignans; D047492:Peroxisome Proliferator-Activated Receptors; D008063:Thioctic Acid; D019833:Genistein; C054125:sesamin