title: Δγ₁134.5 herpes simplex viruses encoding human cytomegalovirus IRS1 or TRS1 induce interferon regulatory factor 3 phosphorylation and an interferon-stimulated gene response.
abstract: The chimeric herpes simplex viruses (HSV) are Δγ₁34.5 vectors encoding the human cytomegalovirus (HCMV) IRS1 or TRS1 genes. They are capable of late viral protein synthesis and are superior to Δγ₁34.5 HSVs in oncolytic activity. The interferon (IFN) response limits efficient HSV gene expression and replication. HCMV TRS1 and IRS1 restore one γ₁34.5 gene function: evasion of IFN-inducible protein kinase R, allowing late viral protein synthesis. Here we show that, unlike wild-type HSV, the chimeric HSV do not restore another γ₁34.5 function, the suppression of early IFN signaling mediated by IFN regulatory factor 3 (IRF3).
authors.affiliation: Department of Pediatrics, Division of Infectious Disease, The University of Alabama at Birmingham, Birmingham, Alabama, USA. kcassady@peds.uab.edu
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Cassady
authors.forename: Ute
authors.initials: U
authors.lastname: Saunders
authors.forename: Masako
authors.initials: M
authors.lastname: Shimamura
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology / Q000502:physiology; D006561:Herpes Simplex / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology; D050130:Oncolytic Virotherapy / Q000295:instrumentation*; D010766:Phosphorylation; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication
chemical_list: C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; C072216:TRS1 protein, Human herpesvirus 5; D014764:Viral Proteins; D016899:Interferon-beta
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