title: Development and application of a method for the analysis of two trichothecenes: deoxynivalenol and T-2 toxin in meat in China by HPLC-MS/MS.
abstract: A reliable and sensitive method was developed and successfully applied for the determination of deoxynivalenol and T-2 toxin simultaneously in pig dorsal muscle, pig back fat and chicken muscle by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) analysis. Limit of detection of deoxynivalenol and T-2 was 0.02μg/kg and 0.007μg/kg, and limit of quantification of deoxynivalenol and T-2 was 0.07μg/kg and 0.02μg/kg, respectively. Sixty-six meat samples were analyzed and deoxynivalenol was detected in the samples of pig back fat, with concentrations lower than 0.5μg/kg, and T-2 toxin was detected in the samples of pig dorsal muscle, pig back fat and chicken muscle, with concentrations lower than 0.5μg/kg. The results of sample analysis show that only trace residues of deoxynivalenol and T-2 toxin were detected in the samples analyzed.
authors.affiliation: College of Food Science, Southwest University, Chongqing 400716, PR China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Zou
authors.forename: Zhifei
authors.initials: Z
authors.lastname: He
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.forename: Pengfei
authors.initials: P
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Cunxian
authors.initials: C
authors.lastname: Xi
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xianliang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D012680:Sensitivity and Specificity; D013552:Swine; D013605:T-2 Toxin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D054928:Validation Studies as Topic
chemical_list: D014255:Trichothecenes; D013605:T-2 Toxin; C007262:deoxynivalenol