title: Culturing INS-1 cells on CDPGYIGSR-, RGD- and fibronectin surfaces improves insulin secretion and cell proliferation.
abstract: Rat insulinoma cells (INS-1), an immortalized pancreatic beta cell line, were cultured on low-fouling carboxymethyl-dextran (CMD) layers bearing fibronectin, the tripeptide Arg-Gly-Asp (RGD) or CDPGYIGSR, a laminin nonapeptide. INS-1 cells were non-adherent on CMD and RGE but adhered to fibronectin- and peptide-coated CMD surfaces and to tissue culture polystyrene (TCPS). On CMD bearing fibronectin and the peptides, INS-1 cells showed higher glucose-stimulated insulin secretion compared to those on TCPS, bare CMD and RGE. INS-1 cells experienced a net cell growth, with the lowest found after 7 days on CMD and the highest on fibronectin. Similarly, cells on RGD and CDPGYIGSR showed lower net growth rates than those on fibronectin. Expression of E-cadherin and integrins αvβ3 and α5 were similar between the conditions, except for α5 expression on fibronectin, RGD and CDPGYIGSR. Larger numbers of Ki-67-positive cells were found on CDPGYIGSR, TCPS, fibronectin and RGD. Cells in all conditions expressed Pdx1.
authors.affiliation: Laboratoire de bio-ingénierie et de biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, boulevard de l'Université, Sherbrooke, Québec, Canada J1K 2R1.
authors.forename: Carina
authors.initials: C
authors.lastname: Kuehn
authors.forename: Evan A
authors.initials: EA
authors.lastname: Dubiel
authors.forename: Georges
authors.initials: G
authors.lastname: Sabra
authors.forename: Patrick
authors.initials: P
authors.lastname: Vermette
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D016023:Integrins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D051381:Rats; D013499:Surface Properties / Q000187:drug effects
chemical_list: C470965:CDPGYIGSR; D015820:Cadherins; D005353:Fibronectins; D007328:Insulin; D016023:Integrins; D019394:Ki-67 Antigen; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid