title: Identification of a novel single-stranded, circular DNA virus from bovine stool.
abstract: We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem-loop structure was located between the 3' ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families Circoviridae, Nanoviridae and Geminiviridae. BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses.
authors.affiliation: Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Hye Kwon
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Van Giap
authors.initials: VG
authors.lastname: Nguyen
authors.affiliation: Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Dae Sub
authors.initials: DS
authors.lastname: Song
authors.affiliation: Research Unit, Green Cross Veterinary Products, Yong-in 449-903, Republic of Korea.
authors.forename: Hyoung Joon
authors.initials: HJ
authors.lastname: Moon
authors.affiliation: Research Unit, Green Cross Veterinary Products, Yong-in 449-903, Republic of Korea.
authors.forename: Bo Kyu
authors.initials: BK
authors.lastname: Kang
authors.affiliation: Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Bong Kyun
authors.initials: BK
authors.lastname: Park
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D016000:Cluster Analysis; D004267:DNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004270:DNA, Circular / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005243:Feces / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D036022:Capsid Proteins; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase