title: The role of metalloendopeptidases in oropharyngeal carcinomas assessed by tissue microarray.
abstract: The goal of this study was to investigate the expression of some metalloendopeptidases in squamous cell carcinomas of the oropharynx as well as its relation to histological differentiation, staging of disease, and prognosis. Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of EP24.15 and EP24.16 by means of tissue microarrays. Expression of EP24.15 or EP24.16 was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinomas of the oropharynx. In summary, our results support the notion that EP24.15 and EP24.16 are expressed in carcinoma of the oropharynx; however, these do not appear to be suitable biomarkers for histological grading, disease stage or recurrence as depicted by tissue microarrays and immunohistochemistry.
authors.affiliation: Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos-SP, Brazil. daribeiro@unifesp.br
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Fabio D
authors.initials: FD
authors.lastname: Nascimento
authors.forename: Ana Carolina C
authors.initials: AC
authors.lastname: Fracalossi
authors.forename: Juliana
authors.initials: J
authors.lastname: Noguti
authors.forename: Celina T F
authors.initials: CT
authors.lastname: Oshima
authors.forename: Silvia S M
authors.initials: SS
authors.lastname: Ihara
authors.forename: Marcello F
authors.initials: MF
authors.lastname: Franco
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000032:analysis*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009959:Oropharyngeal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D009960:Oropharynx / Q000473:pathology; D011379:Prognosis; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D008666:Metalloendopeptidases