title: Wavelet-based image fusion in multi-view three-dimensional microscopy.
abstract: BACKGROUND
  Multi-view microscopy techniques such as Light-Sheet Fluorescence Microscopy (LSFM) are powerful tools for 3D + time studies of live embryos in developmental biology. The sample is imaged from several points of view, acquiring a set of 3D views that are then combined or fused in order to overcome their individual limitations. Views fusion is still an open problem despite recent contributions in the field.
  
  
  RESULTS
  We developed a wavelet-based multi-view fusion method that, due to wavelet decomposition properties, is able to combine the complementary directional information from all available views into a single volume. Our method is demonstrated on LSFM acquisitions from live sea urchin and zebrafish embryos. The fusion results show improved overall contrast and details when compared with any of the acquired volumes. The proposed method does not need knowledge of the system's point spread function (PSF) and performs better than other existing PSF independent fusion methods.
  
  
  METHODS
  The described method was implemented in Matlab (The Mathworks, Inc., USA) and a graphic user interface was developed in Java. The software, together with two sample datasets, is available at http://www.die.upm.es/im/software/SPIMFusionGUI.zip A public release, free of charge for non-commercial use, is planned after the publication of this article.
authors.affiliation: Biomedical Image Technologies, ETSI Telecomunicación, Universidad Politénica de Madrid, Madrid, Spain. jlrubio@die.upm.es
authors.forename: Jose L
authors.initials: JL
authors.lastname: Rubio-Guivernau
authors.forename: Vasily
authors.initials: V
authors.lastname: Gurchenkov
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Luengo-Oroz
authors.forename: Louise
authors.initials: L
authors.lastname: Duloquin
authors.forename: Paul
authors.initials: P
authors.lastname: Bourgine
authors.forename: Andres
authors.initials: A
authors.lastname: Santos
authors.forename: Nadine
authors.initials: N
authors.lastname: Peyrieras
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Ledesma-Carbayo
mesh_terms: D000818:Animals; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D012617:Sea Urchins / Q000196:embryology*; D012984:Software; D015027:Zebrafish / Q000196:embryology*