title: Evaluation of cytotoxic, genotoxic and inflammatory responses of micro- and nano-particles of granite on human lung fibroblast cell IMR-90.
abstract: Occupational exposure of granite workers is well known to cause lung impairment and silicosis. Toxicological profiles of different size particles of granite dust, however, are not yet understood. Present evaluation of micro- and nano-particles of granite dust as on human lung fibroblast cells IMR-90, revealed that their toxic effects were dose-dependent, and nanoparticles in general were more toxic. In this study we first demonstrated that nanoparticles caused oxidative stress, inflammatory response and genotoxicity, as seen by nearly 2 fold induction of ROS and LPO, mRNA levels of TNF-α and IL-1β, and induction in micronuclei formation. All these were significantly higher when compared with the effect of micro particles. Thus, the study suggests that separate health safety standards would be required for granite particles of different sizes.
authors.affiliation: Fibre Toxicology Division, CSIR - Indian Institute of Toxicology Research, Mahatma Gandhi Marg, P.O. Box 80, Lucknow 226001, India. ahmadi@sify.com
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.forename: Mohd Imran
authors.initials: MI
authors.lastname: Khan
authors.forename: Govil
authors.initials: G
authors.lastname: Patil
authors.forename: L K S
authors.initials: LK
authors.lastname: Chauhan
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005434:Flow Cytometry; D005562:Formazans / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008171:Lung Diseases / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015162:Micronucleus Tests; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size; D012822:Silicon Dioxide / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; D017392:Thiobarbituric Acid Reactive Substances; C007886:granite; C079782:MTT formazan; D012822:Silicon Dioxide; D005978:Glutathione