title: ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase.
abstract: Human ATP-citrate lyase (EC 2.3.3.8) is the cytoplasmic enzyme that catalyzes the production of acetyl-CoA from citrate, CoA and ATP. The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the presence of tartrate, ATP and magnesium ions. The crystals diffracted to 2.3 Å resolution. The structure shows ADP-Mg(2+) bound to the domain that possesses the ATP-grasp fold. The structure demonstrates that this crystal form could be used to investigate the structures of complexes with inhibitors of ATP-citrate lyase that bind at either the citrate- or ATP-binding site.
authors.affiliation: Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Sun
authors.forename: Koto
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Marie E
authors.initials: ME
authors.lastname: Fraser
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000737:chemistry* / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000737:chemistry* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D054730:Protein Interaction Domains and Motifs; D040681:Structural Homology, Protein
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D001275:ATP Citrate (pro-S)-Lyase; D008274:Magnesium
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