title: The attachment of intrinsic and extrinsic, mobilized and immobilized adhesion cells to collagen and fibronectin.
abstract: This study investigated the attachment of intrinsic and extrinsic, mobilized and immobilized adhesion cells to the extracellular matrix. Five New Zealand White rabbit forepaws were dissected to isolate the flexor tendon core, tendon surface and synovial sheath, which were explanted separately. A further 10 animals were subjected to flexor tendon injuries, randomized to either mobilization or immobilization, and adhesions were explanted at 2 weeks. Cell groups were tested for attachment to collagen type-I or fibronectin and morphometric analysis was made. The attachment of intrinsic tendon cells and adhesion cells from mobilized tendons to both matrix proteins was statistically significantly greater than that of extrinsic tendon cells and adhesion cells from immobilized tendons. Adhesion cells from mobilized tendons were statistically significantly more elongated, which may correlate with the deposition of a more organized matrix. Because the synovial sheath cells were least attached to matrix proteins, selective treatments that reduce cell attachment may be used to exclude them, without inhibiting intrinsic tendon healing.
authors.affiliation: RAFT, Mount Vernon Hospital, Northwood, Middlesex, UK. olivier.branford@totalise.co.uk
authors.forename: O A
authors.initials: OA
authors.lastname: Branford
authors.forename: D A
authors.initials: DA
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Rolfe
authors.forename: A O
authors.initials: AO
authors.lastname: Grobbelaar
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000502:physiology*; D007103:Immobilization; D011817:Rabbits; D013708:Tendon Injuries
chemical_list: D005353:Fibronectins; D003094:Collagen