title: Detection of tumour necrosis factor-alpha in dogs with lymphoma(*).
abstract: Tumour necrosis factor-alpha (TNF-α) production by malignant lymphoblasts has been identified in vitro and in vivo in mice and humans, respectively. The goals of this study were (1) to evaluate a novel single-sample TNF-α assay and (2) to determine whether TNF-α is increased in dogs with lymphoma prior to and following treatment. Canine TNF-α was analysed concurrently using the novel Siemens Immulite® single-sample automated ELISA and the previously validated Quantikine® standard ELISA. Serum from dogs with lymphoma and from breed-, age- and gender-matched control dogs was evaluated at two time points. Three of 25 (12%) dogs with lymphoma had detectable TNF-α at diagnosis, whereas none had detectable TNF-α following complete or partial remission. TNF-α was not detectable in control dogs. Despite 91% homology between human and canine TNF-α, the Immulite® automated ELISA failed to detect canine TNF-α. Serum TNF-α appears to have limited value as a tumour marker in dogs with lymphoma.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hofer
authors.forename: T C
authors.initials: TC
authors.lastname: DeFrancesco
authors.forename: L E
authors.initials: LE
authors.lastname: Williams
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood* / Q000473:pathology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008223:Lymphoma / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009657:North Carolina; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha