title: Aluminum induces changes in oxidative burst scavenging enzymes in Coffea arabica L. suspension cells with differential Al tolerance.
abstract: The accumulation of reactive oxygen species (ROS) and concomitant oxidative stress have been considered deleterious consequences of aluminum toxicity. However, several lines of evidence suggest that ROS can function as important signaling molecules in the plant defense system for protection from abiotic stress and the acquisition of tolerance. The role of ROS-scavenging enzymes was assayed in two different coffee cell suspension lines. We treated L2 (Al-sensitive) and LAMt (Al-tolerant) Coffea arabica suspension cells with 100 μM AlCl(3) and observed significant differences in catalase activity between the two cell lines. However, we did not observe any differences in superoxide dismutase or glutathione reductase activity in either cell line following Al treatment. ROS production was diminished in the LAMt cell line. Taken together, these results indicate that aluminum treatment may impair the oxidative stress response in L2 cells but not in LAMt cells. We suggest a possible role for Al-induced oxidative bursts in the signaling pathways that lead to Al resistance and protection from Al toxicity.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico.
authors.forename: José Efraín
authors.initials: JE
authors.lastname: Ramírez-Benítez
authors.forename: J Armando
authors.initials: JA
authors.lastname: Muñoz-Sánchez
authors.forename: Karen M
authors.initials: KM
authors.lastname: Becerril-Chi
authors.forename: María de Lourdes
authors.initials: Mde L
authors.lastname: Miranda-Ham
authors.forename: Lizbeth A
authors.initials: LA
authors.lastname: Castro-Concha
authors.forename: S M Teresa
authors.initials: SM
authors.lastname: Hernández-Sotomayor
mesh_terms: D002374:Catalase / Q000378:metabolism*; D018929:Cell Culture Techniques; D040503:Coffea / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D004361:Drug Tolerance; D005980:Glutathione Reductase / Q000378:metabolism*; D018384:Oxidative Stress; D010940:Plant Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase