title: Angelica sinensis suppresses human lung adenocarcinoma A549 cell metastasis             by regulating MMPs/TIMPs and TGF-β1.
abstract: In this study we investigated the potential effects of Angelica sinensis             on the growth and metastasis in human lung adenocarcinoma A549 cells. In vitro             the Cck-8 assays showed that Angelica sinensis had weak antiproliferative effect             on A549 cells only at high concentration. The cell adhesion assay showed that             Angelica sinensis decreased the adhesive ability of A549 cells in a dose- and             time-dependent manner. Transwell invasion and migration assay showed that Angelica             sinensis reduced the invasive and migratory abilities of A549 cells in a dose-dependent             manner. In vivo the animal experiments showed that Angelica sinensis suppressed             lung metastasis of nude mice at high concentration. Then, we attempted to clarify             the mechanisms of anti-metastatic activities of Angelica sinensis. The results             showed Angelica sinensis inhibited the enzymatic activity of matrix metalloproteinase-2             (MMP-2) and matrix metalloproteinase-9 (MMP-9), it involved the down-regulation             of the expressions of MMP-2 and MMP-9 at both the protein and mRNA levels, which             may be associated with Angelica sinensis suppressing the expression of TGF-β1.             It also involved the increase of the tissue inhibitors of metalloproteinases TIMP-2,             but TIMP-1 decreased upon incubation of A549 cells with Angelica sinensis. The             results suggest that Angelica sinensis might exert anti-growth and anti-metastasis             activity against lung cancer cells through the decrease of MMP-2, MMP-9, TGF-β1             and TIMP-1 and increase of TIMP-2.
authors.affiliation: Department of Radio-Chemotherapy, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Fu-Xiang
authors.initials: FX
authors.lastname: Zhou
authors.forename: Cong-Hua
authors.initials: CH
authors.lastname: Xie
authors.forename: Guang
authors.initials: G
authors.lastname: Han
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Fang
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism* / Q000556:secondary; D000077192:Adenocarcinoma of Lung; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D019715:Tissue Inhibitor of Metalloproteinase-1; D019714:Tissue Inhibitor of Metalloproteinases; D053773:Transforming Growth Factor beta1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020782:Matrix Metalloproteinases