title: Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment.
abstract: The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.
authors.affiliation: Laboratory of Microbial Ecology and Technology, Ghent University, Gent, Belgium.
authors.forename: Joan
authors.initials: J
authors.lastname: Vermeiren
authors.forename: Pieter
authors.initials: P
authors.lastname: Van den Abbeele
authors.forename: Debby
authors.initials: D
authors.lastname: Laukens
authors.forename: Louise Kristine
authors.initials: LK
authors.lastname: Vigsnaes
authors.forename: Martine
authors.initials: M
authors.lastname: De Vos
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.forename: Tom
authors.initials: T
authors.lastname: Van de Wiele
mesh_terms: D000214:Acyl Coenzyme A / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D002087:Butyrates / Q000378:metabolism; D003013:Clostridium / Q000254:growth & development / Q000502:physiology*; D015256:Coenzyme A-Transferases / Q000235:genetics / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000378:metabolism / Q000382:microbiology*; D005051:Eubacterium / Q000254:growth & development / Q000502:physiology*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009077:Mucins / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D002087:Butyrates; D005232:Fatty Acids, Volatile; D009077:Mucins; C024343:butyryl-coenzyme A; D015256:Coenzyme A-Transferases