title: Sensitivity of human dental pulp cells to eighteen chemical agents used for endodontic treatments in dentistry.
abstract: To determine the adverse effects against human dental pulp tissue, the sensitivity of human dental pulp cells (D824 cells) to 18 chemical agents used for endodontic treatments in dentistry was examined. The cytotoxicity, as determined by a decrease in colony-forming ability of cells treated with the chemical agents, increased as the concentration increased. As a quantitative measure of the cytotoxic effect, LC(50), the concentration which induces a 50% lethality, was extrapolated from the concentration-response curves. The rank of the chemical agents according to their cytotoxic effect (LC(50)) was sodium arsenite > formaldehyde > hydrogen peroxide > zinc oxide > thymol ≈ iodoform ≈ eugenol > guaiacol > ethylenediaminetetraacetic acid ≈ iodine > procaine > lidocaine ≈ chloramphenicol ≈ m-cresol > calcium hydroxide ≈ sodium hypochlorite ≈ phenol ≈ p-phenolsulfonic acid. To compare the cytotoxicity and the levels of apoptosis and mRNA expression of five genes related to the function of dental pulp tissue, D824 cells treated with the LC(50) concentrations of chemical agents were assayed by the TUNEL method and quantitative reverse transcription polymerase chain reaction analysis, respectively. The inducibility of apoptotic cells and the level of mRNA expression of the genes varied with the chemical agents, indicating that both effects occurred independent of the rank of cytotoxic effect of the chemical agents. The results not only provide information concerning cytotoxicity of various chemical agents to human dental pulp cells, but also show an insight into the diversity of the pharmacodynamic action of the chemical agents.
authors.affiliation: Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, Japan.
authors.forename: Morio
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Takeo W
authors.initials: TW
authors.lastname: Tsutsui
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Maki
authors.initials: M
authors.lastname: Ohno
authors.forename: Yukari
authors.initials: Y
authors.lastname: Higo
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Inaba
authors.forename: Takeki
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000890:Anti-Infective Agents / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D003114:Colony-Forming Units Assay; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003804:Dentin / Q000187:drug effects / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007928:Lethal Dose 50; D011347:Procollagen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011944:Receptors, Androgen / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012388:Root Canal Irrigants / Q000633:toxicity*; D050916:TRPV Cation Channels / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D000890:Anti-Infective Agents; D024042:Collagen Type I; D047628:Estrogen Receptor alpha; D016326:Extracellular Matrix Proteins; D011347:Procollagen; D012333:RNA, Messenger; D011944:Receptors, Androgen; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D000469:Alkaline Phosphatase
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