title: [Establishment of experimental angiogenic models with applications of quantitative digital image analysis].
abstract: OBJECTIVE
  To establish experimental models for tumor neovascularization and to apply quantitative digital imaging analysis in the study.
  
  
  METHODS
  An endothelial tube formation model was established by human umbilical vein endothelial cells (HUVECs). A vasculogenic mimicry model was established by SGC-7901 gastric cancer cell line. Fertilized eggs were used to establish a chorioallantoic membrane angiogenesis model. Using gene transfection experiment, IRX1 tumor suppressor gene was chosen as a therapeutic target. Image Pro Plus (IPP) analysis software was used for digital vascular images analysis with parameters including points, lines, angles and integral absorbance (IA) for the tubular formation or vasculogenic mimicry.
  
  
  RESULTS
  Digital image analysis by IPP showed that HUVEC tubular formation was significantly inhibited in IRX1 transfectant, compared with controls. The tubular numbers in three groups were 12.80 +/- 3.83, 29.00 +/- 5.34 and 28.20 +/- 4.32 (P<0.01). The connection points of tubules in three groups were 13.20 +/- 2.59, 25.00 +/- 2.24 and 24.60 +/- 3.21 (P<0.01). The tubular lengths of three groups were (821.5 +/- 12.5), (930.9 +/- 13.5) and (948.4 +/- 18.1) microm (P=0.022). The IA values of PAS stain in three groups were 3606 +/- 363, 14 200 +/- 1251 and 15 043 +/- 1220 (P<0.01). In chick chorioallantoic membrane model, the angular numbers of tubules in three groups were 6.41 +/- 2.60, 10.27 +/- 2.65 and 9.18 +/- 1.99 (P<0.01).
  
  
  CONCLUSIONS
  The endothelial tube formation model, vasculogenic mimicry model and chorioallantoic membrane angiogenesis model are useful for gene therapy and drug screening with targeting neoplastic vascularization. Professional image analysis software may greatly facilitate the quantitative analysis of tumor neovascularization.
authors.affiliation: Department of Surgery and Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Jiang
authors.forename: Wei-ren
authors.initials: WR
authors.lastname: Liu
authors.forename: Ying-yan
authors.initials: YY
authors.lastname: Yu
authors.forename: Pei-hua
authors.initials: PH
authors.lastname: Ni
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.forename: Jia-nian
authors.initials: JN
authors.lastname: Zhang
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Bing-ya
authors.initials: BY
authors.lastname: Lu
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D049033:Chorioallantoic Membrane / Q000098:blood supply; D003952:Diagnostic Imaging / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic; D012984:Software; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014162:Transfection
chemical_list: D018398:Homeodomain Proteins; C501591:IRX1 protein, human; D014157:Transcription Factors