title: Drosophila TRPA channel painless inhibits male-male courtship behavior through modulating olfactory sensation.
abstract: The Drosophila melanogaster TRPA family member painless, expressed in a subset of multidendritic neurons embeding in the larval epidermis, is necessary for larval nociception of noxious heat or mechanical stimuli. However, the function of painless in adult flies remains largely unknown. Here we report that mutation of painless leads to a defect in male-male courtship behavior and alteration in olfaction sensitivity in adult flies. Specific downregulation of the expression of the Painless protein in the olfactory projection neurons (PNs) of the antennal lobes (ALs) resulted in a phenotype resembling that found in painless mutant flies, whereas overexpression of Painless in PNs of painless mutant males suppressed male-male courtship behavior. The downregulation of Painless exclusively during adulthood also resulted in male-male courtship behavior. In addition, mutation of the painless gene in flies caused changes in olfaction, suggesting a role for this gene in olfactory processing. These results indicate that functions of painless in the adult central nervous system of Drosophila include modulation of olfactory processing and inhibition of male-male courtship behavior.
authors.affiliation: State Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.forename: Yanmeng
authors.initials: Y
authors.lastname: Guo
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D016133:Polymerase Chain Reaction; D012726:Sexual Behavior, Animal; D012903:Smell
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D007473:Ion Channels; C474592:pain protein, Drosophila
references.citation: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13232-7
references.pmid: 12239352
references.citation: Proc Biol Sci. 2006 Feb 7;273(1584):315-23
references.pmid: 16543174
references.citation: Cell. 2003 Apr 18;113(2):261-73
references.pmid: 12705873
references.citation: J Biol Rhythms. 2005 Jun;20(3):237-44
references.pmid: 15851530
references.citation: Nature. 2004 Jan 15;427(6971):260-5
references.pmid: 14712238
references.citation: J Chem Ecol. 1985 Dec;11(12):1747-56
references.pmid: 24311338
references.citation: Nat Neurosci. 2008 Jun;11(6):676-82
references.pmid: 18469811
references.citation: J Neurobiol. 1997 May;32(5):443-56
references.pmid: 9110257
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14668-73
references.pmid: 18787131
references.citation: Cell. 2006 Mar 24;124(6):1269-82
references.pmid: 16564016
references.citation: Genet Res. 2005 Jun;85(3):183-93
references.pmid: 16174337
references.citation: Genes Brain Behav. 2006 Nov;5(8):602-13
references.pmid: 17081265
references.citation: Genes Brain Behav. 2009 Jul;8(5):546-57
references.pmid: 19531155
references.citation: PLoS One. 2011;6(5):e19770
references.pmid: 21625481
references.citation: J Exp Biol. 2005 Mar;208(Pt 5):891-8
references.pmid: 15755887
references.citation: J Neurosci. 2008 May 21;28(21):5539-46
references.pmid: 18495888
references.citation: Nature. 2007 Jul 12;448(7150):151-6
references.pmid: 17625558
references.citation: J Insect Physiol. 2000 Apr;46(4):439-442
references.pmid: 12770207
references.citation: Annu Rev Neurosci. 2006;29:163-201
references.pmid: 16776583
references.citation: Curr Biol. 2009 Aug 25;19(16):R700-13
references.pmid: 19706282
references.citation: Science. 1997 Jun 6;276(5318):1555-8
references.pmid: 9171057
references.citation: Neuron. 2004 Mar 25;41(6):849-57
references.pmid: 15046718
references.citation: Nature. 2008 Mar 27;452(7186):473-7
references.pmid: 18305480
references.citation: Curr Biol. 2005 Sep 6;15(17):1535-47
references.pmid: 16139208
references.citation: J Comp Physiol A. 1985 Sep;157(2):263-77
references.pmid: 3939242
references.citation: Annu Rev Neurosci. 2007;30:505-33
references.pmid: 17506643
references.citation: Genetics. 1997 Nov;147(3):1107-30
references.pmid: 9383056
references.citation: Behav Genet. 2009 Sep;39(5):554-63
references.pmid: 19618260
references.citation: Cell. 2004 Jun 25;117(7):981-91
references.pmid: 15210117
references.citation: Nature. 2003 Jun 19;423(6942):822-3
references.pmid: 12815418
references.citation: Science. 2003 Dec 5;302(5651):1765-8
references.pmid: 14657498
references.citation: Curr Biol. 2006 May 23;16(10):1034-40
references.pmid: 16647259
references.citation: Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10446-51
references.pmid: 8816820
references.citation: Curr Biol. 2009 Aug 11;19(15):1245-54
references.pmid: 19615904
references.citation: Curr Biol. 2008 Sep 23;18(18):R880-9
references.pmid: 18812089
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13606-11
references.pmid: 19666538
references.citation: PLoS One. 2007 Aug 15;2(7):e661
references.pmid: 17710124