title: [Development and application of the physical hypoxic models of C. elegans].
abstract: OBJECTIVE
  To develop a suitable hypoxic injury model, which is important for revealing pathological molecular mechanism of hypoxia.
  
  
  METHODS
  We focused on C. elegans by treatment with different hypoxic times and systematically observed mortality, movement, Cellular morphology and the related-protein expression of the animals.
  
  
  RESULTS
  We demonstrated that hypoxia (0.2% partial pressure of oxygen) induced morphological cell defects, and then leading to death of C. elegans. The mortality of C. elegans increased along with hypoxic time, while hypoxia-inducible factor (HIF-1) was significantly up-regulated. In addition, by using neuron-specific transgenic wonns with green fluorescent protein--we observed the neuron-specffic injury caused by hypoxic stress.
  
  
  CONCLUSIONS
  We successfully established an effective, convenient physical hypoxic model of C. elegans, which will facilitate the studies of hypoxic pathology and molecular mechanisms of hypoxic response in the future.
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, State Key Laboratory of Medical Neurobiology, Beijing 100850, China.
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Ren
authors.forename: Ji-ye
authors.initials: JY
authors.lastname: Zhang
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.forename: Hu-qi
authors.initials: HQ
authors.lastname: Liu
authors.forename: Cheng-gang
authors.initials: CG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D000860:Hypoxia / Q000503:physiopathology*; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism; D009474:Neurons / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C541178:HIF-1 protein, C elegans; D051793:Hypoxia-Inducible Factor 1; D014157:Transcription Factors