title: Precision-cut slice culture method for rat placenta.
abstract: Primary trophoblasts, placental explants, and cell line cultures are commonly used to investigate placental development, physiology, and pathology, particularly in relation to pregnancy outcomes. Organotypic slice cultures are increasingly used in other systems because they maintain the normal three-dimensional tissue architecture and have all cell types represented. Herein, we demonstrate the utility of the precision-cut placental slice culture model for studying trophoblastic diseases.
authors.affiliation: Department of Medicine, Liver Research Center, Rhode Island Hospital, Providence, RI, USA.
authors.forename: J
authors.initials: J
authors.lastname: Gilligan
authors.forename: M
authors.initials: M
authors.lastname: Tong
authors.forename: L
authors.initials: L
authors.lastname: Longato
authors.forename: S M
authors.initials: SM
authors.lastname: de la Monte
authors.forename: F
authors.initials: F
authors.lastname: Gundogan
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology; D005240:Feasibility Studies; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D009336:Necrosis; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D013997:Time Factors; D046509:Tissue Culture Techniques; D018675:Toxicity Tests
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D018528:ATP-Binding Cassette Transporters; C489249:Abcg2 protein, rat; D012333:RNA, Messenger; D000431:Ethanol
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