title: Functional characterization of a synthetic abscisic acid analog with anti-inflammatory activity on human granulocytes and monocytes.
abstract: The phytohormone abscisic acid (ABA), in addition to regulating several important physiological functions in plants, is also produced and released by human granulocytes and monocytes where it stimulates cell activities involved in the innate immune response. Here we describe the properties of an ABA synthetic analog that competes with the hormone for binding to human granulocyte membranes and to purified recombinant LANCL2 (the human ABA receptor) and inhibits several ABA-triggered inflammatory functions of granulocytes and monocytes in vitro: chemotaxis, phagocytosis, reactive oxygen species production and release of prostaglandin E(2) (PGE(2)) by human granulocytes, release of PGE(2) and of monocyte chemoattractant protein-1 by human monocytes. This observation provides a proof of principle that ABA antagonists may represent a new class of anti-inflammatory agents.
authors.affiliation: Department of Experimental Medicine (DIMES), Section of Biochemistry, University of Genova, Viale Benedetto XV 1, 16132 Genova, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Grozio
authors.forename: Enrico
authors.initials: E
authors.lastname: Millo
authors.forename: Lucrezia
authors.initials: L
authors.lastname: Guida
authors.forename: Tiziana
authors.initials: T
authors.lastname: Vigliarolo
authors.forename: Marta
authors.initials: M
authors.lastname: Bellotti
authors.forename: Annalisa
authors.initials: A
authors.lastname: Salis
authors.forename: Chiara
authors.initials: C
authors.lastname: Fresia
authors.forename: Laura
authors.initials: L
authors.lastname: Sturla
authors.forename: Mirko
authors.initials: M
authors.lastname: Magnone
authors.forename: Andrea
authors.initials: A
authors.lastname: Galatini
authors.forename: Gianluca
authors.initials: G
authors.lastname: Damonte
authors.forename: Antonio
authors.initials: A
authors.lastname: De Flora
authors.forename: Santina
authors.initials: S
authors.lastname: Bruzzone
authors.forename: Luca
authors.initials: L
authors.lastname: Bagnasco
authors.forename: Elena
authors.initials: E
authors.lastname: Zocchi
mesh_terms: D000040:Abscisic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000494:pharmacology*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D006098:Granulocytes / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects*; D009687:Nuclear Proteins / Q000737:chemistry; D010587:Phagocytosis / Q000187:drug effects; D028044:Phosphate-Binding Proteins; D011994:Recombinant Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: C569503:2',3'-dihydro-2',3'-epoxyabscisic acid; D000894:Anti-Inflammatory Agents, Non-Steroidal; C471704:LANCL2 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D011994:Recombinant Proteins; D000040:Abscisic Acid