title: Development of novel nanocarrier-based near-infrared optical probes for in vivo tumor imaging.
abstract: Optical imaging with near-infrared (NIR) fluorescent probes is a useful diagnostic technology for in vivo tumor detection. Our plan was to develop novel NIR fluorophore-micelle complex probes. IC7-1 and IC7-2 were synthesized as novel lipophilic NIR fluorophores, which were encapsulated in an amphiphilic polydepsipeptide micelle "lactosome". The fluorophore-micelle complexes IC7-1 lactosome and IC7-2 lactosome were evaluated as NIR fluorescent probes for in vivo tumor imaging. IC7-1 and IC7-2 were synthesized and then encapsulated in lactosomes. The optical properties of IC7-1, IC7-2, IC7-1 lactosome and IC7-2 lactosome were measured. IC7-1 lactosome and IC7-2 lactosome were administered to tumor-bearing mice, and fluorescence images were acquired for 48 h. IC7-1 and IC7-2 were successfully synthesized in 12% and 6.3% overall yield, and maximum emission wavelengths in chloroform were observed at 858 nm and 897 nm, respectively. Aqueous buffered solutions of IC7-1 lactosome and IC7-2 lactosome showed similar fluorescence spectra in chloroform and higher or comparable quantum yields and higher photostability compared with ICG. Both lactosome probes specifically visualized tumor tissue 6 h post-administration. IC7-1 lactosome and IC7-2 lactosome could be promising NIR probes for in vivo tumor imaging.
authors.affiliation: Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Takashi
authors.initials: T
authors.lastname: Temma
authors.forename: Isao
authors.initials: I
authors.lastname: Hara
authors.forename: Ryo
authors.initials: R
authors.lastname: Yamahara
authors.forename: Ei-ichi
authors.initials: E
authors.lastname: Ozeki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ono
authors.forename: Hideo
authors.initials: H
authors.lastname: Saji
mesh_terms: D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008823:Micelles; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D019265:Spectroscopy, Near-Infrared
chemical_list: D005456:Fluorescent Dyes; D008823:Micelles
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