title: Purification and inflammatory edema induced by two PLA2 (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae).
abstract: The Anch TX-I and II PLA(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on μ-Bondapak C18 column. Both PLA(2) showed a molecular mass of ~14kDa determined by MALDI-TOF mass spectrometry and showed a high catalytic activity (data not showed). Although homologous with mammalian or snake venom group I PLA(2)s, Anch TX-I and II is sufficiently structurally different for the question of its placement into the existing PLA(2) classification scheme to arise. In addition, Anch TX-I and II, despite possessing many common structural features, also differ in some important structural properties. The amino acid sequence of both PLA(2) (Anch TX-I and III) showed high amino acid sequence identity with PLA(2)Rhopilema nomadica and Bunodosoma caissarum Cnidaria and PLA(2) of group III protein isolated from the Mexican lizard Heloderma horridum horridum and Heloderma suspectum. In addition, Anch TX-I and Anch TX-II showed high amino acid sequence identity with PLA(2) from group III also showed significant overall homology to bee Apis dorsata, Bombus terrestris and Bombus pennsylvanicus and PLA(2). We also investigated the in vivo edematogenic activity of Anch TX-I and Anch TX-II in a model of paw and skin edema in rats and observed that both are able to induce dose-dependent edema.
authors.affiliation: Departamento de Farmacologia, Faculdade de Ciências Médicas, CEP 13083-970, Campinas, SP, Brazil.
authors.forename: Elen Cristina Teizem
authors.initials: EC
authors.lastname: Landucci
authors.forename: Queila Cristina
authors.initials: QC
authors.lastname: Dias
authors.forename: Fábio André
authors.initials: FA
authors.lastname: Marangoni
authors.forename: Augusto
authors.initials: A
authors.lastname: Vilca-Quispe
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Valeriano-Zapana
authors.forename: Frank Denis
authors.initials: FD
authors.lastname: Torres-Huaco
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Sergio
authors.initials: S
authors.lastname: Marangoni
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Ponce-Soto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D056148:Chromatography, Reverse-Phase; D004487:Edema / Q000139:chemically induced*; D005528:Foot / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D008978:Mollusk Venoms / Q000201:enzymology*; D054467:Phospholipases A2 / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D059748:Proteolysis; D051381:Rats; D017208:Rats, Wistar; D012615:Sea Anemones / Q000201:enzymology*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D012867:Skin / Q000187:drug effects / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008978:Mollusk Venoms; D054467:Phospholipases A2