title: Ozonated autohemotherapy: protection of kidneys from ischemia in rats subjected to unilateral nephrectomy.
abstract: BACKGROUND
  Ozonated autohemotherapy (OA) has been previously successfully used in the treatment of patients affected by peripheral occlusive arterial disease. OA consists of an intrafemoral reinfusion of autologous blood previously exposed to a mixture of oxygen/ozone (O2/O3). This study analyzes the effects of OA in protecting rat kidney from ischemia and ischemia/reperfusion damage.
  
  
  METHODS
  We performed OA 30 min before the induction of 60 min renal ischemia or at the induction of 60 min postischemic reperfusion in rats subjected to unilateral nephrectomy. In addition, to evidence the possible protection induced by O2/O3 on endothelial functions, the present study analyzes the in vitro effects of O2/O3 on oxygen consumption by human umbilical vein endothelial cells (HUVEC).
  
  
  RESULTS
  1) OA preserves rat kidney functions and architecture, as demonstrated by the improved levels of serum creatinine and blood urea nitrogen and by histology; 2) such protection does not correlate with the increase of plasmatic nitric oxide, but is compatible with a focal renal increase of renal βNADPH-diaphorase; 3) treatment of HUVEC with O2/O3 significantly increases both the rate of oxygen consumption and the mitochondrial activity assessed by confocal microscopy.
  
  
  CONCLUSIONS
  The preservation of the mitochondrial activity of endothelium could in vivo limit the endothelial dysfunction provoked by the Isc or Isc/R processes.
authors.affiliation: Clinical Cardiovascular Biology Laboratory, San Raffaele Scientific Institute, Milano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Foglieni
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fulgenzi
authors.forename: Daniela
authors.initials: D
authors.lastname: Belloni
authors.forename: Clara
authors.initials: C
authors.lastname: Sciorati
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ferrero
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Ferrero
mesh_terms: D000818:Animals; D001804:Blood Transfusion, Autologous / Q000379:methods*; D007261:Infusions, Intra-Arterial; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D009392:Nephrectomy / Q000009:adverse effects*; D010126:Ozone / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000503:physiopathology* / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D010126:Ozone
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