title: Covariation of major and minor viral capsid proteins in norovirus genogroup II genotype 4 strains.
abstract: We report sequence hypervariability in the viral protein 1 (VP1) interaction domain of VP2 in the norovirus (NoV) genogroup II genotype 4 (GII.4) lineage on 3 levels: (i) the global evolution of pandemic/epidemic strains from the mid-1970s through post-2006, (ii) the local emergence of an epidemic strain, and (iii) an immunocompromised patient chronically shedding NoV. When a quantitative yeast two-hybrid assay was used, VP2 was found to interact with VP1 in a time-ordered, strain-dependent manner among 3 NoV GII.4 strains. Our findings suggest that VP1 and VP2 may covary in virus evolution and that sequence hypervariability of VP2 may be functionally driven. Further investigations are warranted.
authors.affiliation: Institute of Digestive Disease, The Chinese University of Hong Kong, People’s Republic of China. martin.chan@cuhk.edu.hk
authors.forename: Martin Chi-Wai
authors.initials: MC
authors.lastname: Chan
authors.forename: Nelson
authors.initials: N
authors.lastname: Lee
authors.forename: Wing-Shan
authors.initials: WS
authors.lastname: Ho
authors.forename: Carmen Oi-Kwan
authors.initials: CO
authors.lastname: Law
authors.forename: Terrence Chi-Kong
authors.initials: TC
authors.lastname: Lau
authors.forename: Stephen Kwok-Wing
authors.initials: SK
authors.lastname: Tsui
authors.forename: Joseph Jao-Yiu
authors.initials: JJ
authors.lastname: Sung
mesh_terms: D017250:Caliciviridae Infections / Q000821:virology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins
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