title: N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes.
abstract: Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP β) and peroxisomal proliferator activated receptor gamma (PPAR γ) expression; we suggested that intracellular GSH content could be responsible for these effects.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires 1428, Argentina; E-Mails:  pablo_calza@hotmail.com  (P.C.);  daianasapochnik@hotmail.com  (D.S.);  solecosen@hotmail.com  (S.C.);  juancalvo@ibyme.conicet.gov.ar  (J.C.C.).
authors.forename: Pablo
authors.initials: P
authors.lastname: Calzadilla
authors.forename: Daiana
authors.initials: D
authors.lastname: Sapochnik
authors.forename: Soledad
authors.initials: S
authors.lastname: Cosentino
authors.forename: Virginia
authors.initials: V
authors.lastname: Diz
authors.forename: Lelia
authors.initials: L
authors.lastname: Dicelio
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Calvo
authors.forename: Liliana N
authors.initials: LN
authors.lastname: Guerra
mesh_terms: D041721:3T3-L1 Cells; D000111:Acetylcysteine / Q000378:metabolism*; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002454:Cell Differentiation; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D015415:Biomarkers; D022782:CCAAT-Enhancer-Binding Protein-beta; D047495:PPAR gamma; D017382:Reactive Oxygen Species; D014280:Triglycerides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D000111:Acetylcysteine
references.citation: J Biol Chem. 1998 Nov 13;273(46):30057-60
references.pmid: 9804754
references.citation: Annu Rev Biochem. 1995;64:345-73
references.pmid: 7574486
references.citation: J Biol Chem. 2005 Feb 11;280(6):4617-26
references.pmid: 15536073
references.citation: Antioxid Redox Signal. 2006 Mar-Apr;8(3-4):243-70
references.pmid: 16677071
references.citation: Nature. 2001 Dec 13;414(6865):813-20
references.pmid: 11742414
references.citation: Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):850-5
references.pmid: 12525691
references.citation: Am J Physiol Endocrinol Metab. 2009 Jun;296(6):E1326-34
references.pmid: 19366877
references.citation: Growth Horm IGF Res. 2010 Jun;20(3):212-9
references.pmid: 20185348
references.citation: J Clin Invest. 2004 Dec;114(12):1752-61
references.pmid: 15599400
references.citation: Free Radic Biol Med. 2008 Aug 1;45(3):231-41
references.pmid: 18482593
references.citation: J Biol Chem. 1969 Nov 25;244(22):6049-55
references.pmid: 5389100
references.citation: Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8852-7
references.pmid: 15184668
references.citation: J Biol Chem. 2009 Apr 17;284(16):10601-9
references.pmid: 19237544
references.citation: Br J Nutr. 2008 Jan;99(1):37-43
references.pmid: 17651528
references.citation: Free Radic Biol Med. 2002 Feb 1;32(3):233-45
references.pmid: 11827749
references.citation: Biofactors. 2009 Jul-Aug;35(4):373-9
references.pmid: 19353690
references.citation: Anal Biochem. 1990 Nov 1;190(2):360-5
references.pmid: 2291479
references.citation: Redox Rep. 2008;13(4):185-91
references.pmid: 18647489
references.citation: Free Radic Biol Med. 2007 Apr 1;42(7):1089-97
references.pmid: 17349935
references.citation: J Biol Chem. 1985 Mar 10;260(5):2646-52
references.pmid: 3882699
references.citation: J Agric Food Chem. 2007 Oct 17;55(21):8404-10
references.pmid: 17880164
references.citation: J Biol Chem. 2004 Sep 24;279(39):40462-9
references.pmid: 15265861
references.citation: J Lab Clin Med. 1967 Jul;70(1):158-69
references.pmid: 6066618
references.citation: Clin Chim Acta. 2005 Feb;352(1-2):115-20
references.pmid: 15653105
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: Methods Enzymol. 2005;401:379-407
references.pmid: 16399399
references.citation: FASEB J. 1995 Aug;9(11):995-1003
references.pmid: 7649415
references.citation: Mol Cell Biol. 2004 Oct;24(19):8671-80
references.pmid: 15367685
references.citation: Proc Natl Acad Sci U S A. 2009 May 26;106(21):8665-70
references.pmid: 19433800
references.citation: Life Sci. 2006 Nov 17;79(25):2405-12
references.pmid: 16952378
references.citation: J Nutr. 2009 Jan;139(1):51-7
references.pmid: 19056650
references.citation: J Biol Chem. 2001 Dec 28;276(52):48662-9
references.pmid: 11598110
references.citation: Prog Lipid Res. 2003 Jul;42(4):318-43
references.pmid: 12689622
references.citation: Free Radic Biol Med. 2009 Nov 15;47(10):1432-9
references.pmid: 19703550
references.citation: Nutr Metab (Lond). 2006 Jul 27;3:30
references.pmid: 16872526
references.citation: Mol Cell Biol. 2010 Aug;30(16):4077-91
references.pmid: 20530198
references.citation: Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12446-50
references.pmid: 11050169
references.citation: Exp Mol Med. 2006 Apr 30;38(2):162-72
references.pmid: 16672770
references.citation: Exp Cell Res. 1991 Oct;196(2):172-6
references.pmid: 1893932
references.citation: IUBMB Life. 2001 Feb;51(2):105-9
references.pmid: 11463161
references.citation: Physiol Rev. 2002 Jan;82(1):47-95
references.pmid: 11773609
references.citation: Science. 1980 Nov 28;210(4473):978-84
references.pmid: 6254151
references.citation: Life Sci. 1985 Apr 22;36(16):1569-75
references.pmid: 3982227
references.citation: Clin Chim Acta. 2003 Dec;338(1-2):123-9
references.pmid: 14637276
references.citation: Cancer Res. 2000 Mar 15;60(6):1604-8
references.pmid: 10749129
references.citation: Free Radic Biol Med. 2006 Jun 1;40(11):1929-38
references.pmid: 16716894
references.citation: J Immunol Methods. 1999 Dec 17;232(1-2):3-14
references.pmid: 10618505
references.citation: Trends Pharmacol Sci. 2006 Dec;27(12):639-45
references.pmid: 17056127
references.citation: Adv Pharmacol. 1997;38:205-27
references.pmid: 8895810
references.citation: Arch Biochem Biophys. 2003 Dec 15;420(2):262-7
references.pmid: 14654065
references.citation: Biocell. 2007 Aug;31(2):237-45
references.pmid: 17902272
references.citation: Biochim Biophys Acta. 2006 Dec;1763(12):1755-66
references.pmid: 17034877