title: A thin-layer chromatography plate prepared from BODIPY-based receptor immobilized SiO2 nanoparticles as a portable chemosensor for Pb2+.
abstract: A new fluorescence receptor based on BODIPY-immobilized silica nanoparticles (BODIPY-SiO(2)) exhibits a high affinity and selectivity for Pb(2+) over competing metal ions in water. An overall emission change of ca. 100-fold at the emission maximum was observed for Pb(2+). The fluorescence receptor BODIPY-SiO(2) can remove 97% and 95% of the initial 100 ppb Pb(2+) from human blood and waste solution, respectively. Experiments show the fluorescence receptor BODIPY-SiO(2) can be a potentially useful and effective agent for the selective separation and rapid removal of Pb(2+)in vivo. We also prepared a portable chemosensor kit by coating a 4 μm thick film of BODIPY-SiO(2) onto a glass substrate. We found that this BODIPY-SiO(2) film detects Pb(2+) ions at pH 7.4 with a sensitivity of 3.2 nM. Finally, we tested the effect of pH on BODIPY-SiO(2) with Pb(2+) ions between pH 3.0 and 11.0. The fluorescence changes of BODIPY-SiO(2) were almost constant between pH 3 and 11. The results imply that the BODIPY-SiO(2) film is applicable as a portable chemosensor for detection of Pb(2+) ions in the environmental field.
authors.affiliation: Department of Chemistry, Research Institute of Natural Science, Gyeongsang National University, Jinju, 660-701, Korea.
authors.forename: Hyunjong
authors.initials: H
authors.lastname: Son
authors.forename: Gyusik
authors.initials: G
authors.lastname: Kang
authors.forename: Jong Hwa
authors.initials: JH
authors.lastname: Jung
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001896:Boron Compounds / Q000737:chemistry*; D002413:Cations, Divalent / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013057:Spectrum Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000302:isolation & purification
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D014874:Water Pollutants, Chemical; D007854:Lead; D012822:Silicon Dioxide