title: Genetic variations in the CYP17A1 and NT5C2 genes are associated with a reduction in visceral and subcutaneous fat areas in Japanese women.
abstract: Visceral fat accumulation has an important role in increasing the morbidity and mortality rates, by increasing the risk of developing several metabolic disorders, such as type 2 diabetes, dyslipidemia and hypertension. New genetic loci that are associated with increased systolic and diastolic blood pressures have been identified by genome-wide association studies in Caucasian populations. This study investigates whether single nucleotide polymorphisms (SNPs) that confer susceptibility to high blood pressure are also associated with visceral fat obesity. We genotyped 1279 Japanese subjects (556 men and 723 women) who underwent computed tomography for measuring the visceral fat area (VFA) and subcutaneous fat area (SFA) at the following SNPs: FGF5 rs16998073, CACNB2 rs11014166, C10orf107 rs1530440, CYP17A1 rs1004467, NT5C2 rs11191548, PLEKHA7 rs381815, ATP2B1 rs2681472 and rs2681492, ARID3B rs6495112, CSK rs1378942, PLCD3 rs12946454, and ZNF652 rs16948048. In an additive model, risk alleles of the CYP17A1 rs1004467 and NT5C2 rs11191548 were found to be significantly associated with reduced SFA (P=0.00011 and 0.0016, respectively). When the analysis was performed separately in men and women, significant associations of rs1004467 (additive model) and rs11191548 (recessive model) with reduced VFA (P=0.0018 and 0.0022, respectively) and SFA (P=0.00039 and 0.00059, respectively) were observed in women, but not in men. Our results suggest that polymorphisms in the CYP17A1 and NT5C2 genes influence a reduction in both visceral and subcutaneous fat mass in Japanese women.
authors.affiliation: EBM Research Center, Kyoto University Graduate School of Medicine, Yoshida-Konoecho, Sakyo-ku, Kyoto, Japan. kikukoh@kuhp.kyoto-u.ac.jp
authors.forename: Kikuko
authors.initials: K
authors.lastname: Hotta
authors.forename: Aya
authors.initials: A
authors.lastname: Kitamoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Kitamoto
authors.forename: Seiho
authors.initials: S
authors.lastname: Mizusawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Teranishi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Matsuo
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakata
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Hyogo
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ochi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Seika
authors.initials: S
authors.lastname: Kamohara
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Miyatake
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kotani
authors.forename: Ryoya
authors.initials: R
authors.lastname: Komatsu
authors.forename: Naoto
authors.initials: N
authors.lastname: Itoh
authors.forename: Ikuo
authors.initials: I
authors.lastname: Mineo
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Masato
authors.initials: M
authors.lastname: Yoneda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Katsuto
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.forename: Takato
authors.initials: T
authors.lastname: Ueno
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hamaguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hanafusa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
authors.forename: Toshiie
authors.initials: T
authors.lastname: Sakata
authors.forename: Kiyoji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuzawa
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sekine
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics*; D050154:Adiposity / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics; D015992:Body Mass Index; D005260:Female; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D050152:Intra-Abdominal Fat / Q000201:enzymology*; D007564:Japan; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics*; D050151:Subcutaneous Fat / Q000201:enzymology*
chemical_list: C501892:CYP17A1 protein, human; D013254:Steroid 17-alpha-Hydroxylase; D015720:5'-Nucleotidase; C508048:NT5C2 protein, human