title: Abundance and composition of epiphytic bacterial and archaeal ammonia oxidizers of marine red and brown macroalgae.
abstract: Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are important for nitrogen cycling in marine ecosystems. Little is known about the diversity and abundance of these organisms on the surface of marine macroalgae, despite the algae's potential importance to create surfaces and local oxygen-rich environments supporting ammonia oxidation at depths with low dissolved oxygen levels. We determined the abundance and composition of the epiphytic bacterial and archaeal ammonia-oxidizing communities on three species of macroalgae, Osmundaria volubilis, Phyllophora crispa, and Laminaria rodriguezii, from the Balearic Islands (western Mediterranean Sea). Quantitative PCR of bacterial and archaeal 16S rRNA and amoA genes was performed. In contrast to what has been shown for most other marine environments, the macroalgae's surfaces were dominated by bacterial amoA genes rather than those from the archaeal counterpart. On the basis of the sequences retrieved from AOB and AOA amoA gene clone libraries from each algal species, the bacterial ammonia-oxidizing communities were related to Nitrosospira spp. and to Nitrosomonas europaea and only 6 out of 15 operational taxonomic units (OTUs) were specific for the host species. Conversely, the AOA diversity was higher (43 OTUs) and algal species specific, with 17 OTUs specific for L. rodriguezii, 3 for O. volubilis, and 9 for P. crispa. Altogether, the results suggest that marine macroalgae may exert an ecological niche for AOB in marine environments, potentially through specific microbe-host interactions.
authors.affiliation: Molecular Microbial Ecology Group, Institute of Aquatic Ecology, Universitat de Girona, Girona, Spain.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Trias
authors.forename: Arantzazu
authors.initials: A
authors.lastname: García-Lledó
authors.forename: Noemí
authors.initials: N
authors.lastname: Sánchez
authors.forename: José Luis
authors.initials: JL
authors.lastname: López-Jurado
authors.forename: Sara
authors.initials: S
authors.lastname: Hallin
authors.forename: Lluís
authors.initials: L
authors.lastname: Bañeras
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007795:Laminaria / Q000382:microbiology*; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000461:Rhodophyta / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase
references.citation: ISME J. 2007 Nov;1(7):660-2
references.pmid: 18043673
references.citation: Arch Microbiol. 1995 Sep;164(3):165-72
references.pmid: 7545384
references.citation: Nature. 2006 Aug 17;442(7104):806-9
references.pmid: 16915287
references.citation: Appl Environ Microbiol. 1980 Sep;40(3):578-86
references.pmid: 16345636
references.citation: Appl Environ Microbiol. 2009 Dec;75(23):7537-41
references.pmid: 19801464
references.citation: Environ Microbiol. 2008 Nov;10(11):2931-41
references.pmid: 18973620
references.citation: Appl Environ Microbiol. 2002 Mar;68(3):1015-24
references.pmid: 11872445
references.citation: Environ Microbiol. 2011 Mar;13(3):655-65
references.pmid: 21078035
references.citation: Environ Microbiol. 2008 Nov;10(11):2942-55
references.pmid: 18363713
references.citation: J Microbiol Methods. 2004 Jun;57(3):399-407
references.pmid: 15134887
references.citation: Environ Microbiol. 2008 May;10(5):1357-64
references.pmid: 18325029
references.citation: Nature. 2005 Sep 22;437(7058):543-6
references.pmid: 16177789
references.citation: Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14683-8
references.pmid: 16186488
references.citation: Appl Microbiol Biotechnol. 2010 Jan;85(3):425-40
references.pmid: 19830422
references.citation: Environ Microbiol. 2008 Nov;10(11):3002-16
references.pmid: 18973621
references.citation: Appl Environ Microbiol. 2008 Apr;74(7):2218-28
references.pmid: 18263752
references.citation: ISME J. 2011 Apr;5(4):590-600
references.pmid: 21048801
references.citation: ISME J. 2009 May;3(5):597-605
references.pmid: 19148144
references.citation: FEMS Microbiol Ecol. 2008 Apr;64(1):65-77
references.pmid: 18328081
references.citation: ISME J. 2010 Feb;4(2):301-11
references.pmid: 19829319
references.citation: Appl Environ Microbiol. 2010 Nov;76(22):7626-34
references.pmid: 20889787
references.citation: FEMS Microbiol Rev. 2009 Sep;33(5):855-69
references.pmid: 19453522
references.citation: Nat Rev Microbiol. 2008 Mar;6(3):245-52
references.pmid: 18274537
references.citation: Adv Microb Physiol. 2010;57:1-41
references.pmid: 21078440
references.citation: Biofouling. 2010;26(3):247-55
references.pmid: 20054721
references.citation: Environ Microbiol. 2007 May;9(5):1162-75
references.pmid: 17472632
references.citation: Nature. 2001 Jan 25;409(6819):507-10
references.pmid: 11206545
references.citation: Appl Environ Microbiol. 1997 Dec;63(12):4704-12
references.pmid: 9406389
references.citation: Appl Environ Microbiol. 2009 May;75(10):3127-36
references.pmid: 19304820
references.citation: Environ Microbiol. 2003 Sep;5(9):787-97
references.pmid: 12919414
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12317-22
references.pmid: 16894176
references.citation: Mol Biol Evol. 2007 Aug;24(8):1596-9
references.pmid: 17488738
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):8228-35
references.pmid: 16332807
references.citation: Proc Natl Acad Sci U S A. 2011 May 17;108(20):8420-5
references.pmid: 21525411
references.citation: ISME J. 2010 Jan;4(1):38-48
references.pmid: 19617876
references.citation: Appl Environ Microbiol. 2000 Dec;66(12):5368-82
references.pmid: 11097916
references.citation: Nucleic Acids Res. 2001 Jan 1;29(1):181-4
references.pmid: 11125085
references.citation: FEMS Microbiol Ecol. 2010 Jul 1;73(1):17-30
references.pmid: 20402776
references.citation: Environ Microbiol. 2008 Apr;10(4):1087-94
references.pmid: 18177367
references.citation: Appl Environ Microbiol. 2007 Sep;73(17):5642-7
references.pmid: 17586663