title: Transmissible gastroenteritis coronavirus RNA-dependent RNA polymerase and nonstructural proteins 2, 3, and 8 are incorporated into viral particles.
abstract: Coronavirus replication and transcription are processes mediated by a protein complex, with the RNA-dependent RNA polymerase (RdRp) as a main component. Proteomic analysis of highly purified transmissible gastroenteritis virus showed the RdRp to be a component of the viral particles. This finding was confirmed by Western blotting, immunofluorescence, and immunoelectron microscopy analyses. Interestingly, the replicase nonstructural proteins 2, 3, and 8 colocalized with the RdRp in the viral factories and were also incorporated into the virions.
authors.affiliation: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain.
authors.forename: Aitor
authors.initials: A
authors.lastname: Nogales
authors.forename: Silvia
authors.initials: S
authors.lastname: Márquez-Jurado
authors.forename: Carmen
authors.initials: C
authors.lastname: Galán
authors.forename: Luis
authors.initials: L
authors.lastname: Enjuanes
authors.forename: Fernando
authors.initials: F
authors.lastname: Almazán
mesh_terms: D000818:Animals; D005761:Gastroenteritis, Transmissible, of Swine / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins; D012324:RNA-Dependent RNA Polymerase
references.citation: J Virol. 2003 Oct;77(19):10515-27
references.pmid: 12970436
references.citation: Arch Virol. 2010;155(1):133-46
references.pmid: 19960211
references.citation: RNA Biol. 2011 Mar-Apr;8(2):237-48
references.pmid: 21378501
references.citation: J Virol. 1995 Sep;69(9):5269-77
references.pmid: 7636969
references.citation: Adv Virus Res. 2006;66:193-292
references.pmid: 16877062
references.citation: J Virol. 2008 Jun;82(11):5279-94
references.pmid: 18367524
references.citation: Proteome Sci. 2010 Jun 09;8:29
references.pmid: 20534109
references.citation: Virology. 2009 Sep 1;391(2):304-14
references.pmid: 19580983
references.citation: J Virol. 1986 Oct;60(1):131-9
references.pmid: 2427744
references.citation: J Virol. 2010 Feb;84(4):2169-75
references.pmid: 19955314
references.citation: J Virol. 1996 Jul;70(7):4773-7
references.pmid: 8676505
references.citation: J Virol. 2005 Jun;79(11):6620-30
references.pmid: 15890900
references.citation: PLoS Biol. 2008 Sep 16;6(9):e226
references.pmid: 18798692
references.citation: J Virol Methods. 2011 Jul;175(1):7-13
references.pmid: 21513742
references.citation: J Gen Virol. 2000 Apr;81(Pt 4):853-79
references.pmid: 10725411
references.citation: Annu Rev Microbiol. 2006;60:211-30
references.pmid: 16712436
references.citation: Virus Res. 1988 Apr;10(1):77-93
references.pmid: 2453977
references.citation: Nucleic Acids Res. 1989 Jun 26;17(12):4847-61
references.pmid: 2526320
references.citation: J Virol. 2002 Apr;76(8):3697-708
references.pmid: 11907209
references.citation: J Virol. 2004 Nov;78(22):12683-8
references.pmid: 15507657
references.citation: Virology. 2007 Nov 25;368(2):296-308
references.pmid: 17692883
references.citation: J Virol. 2006 Jun;80(12):5927-40
references.pmid: 16731931
references.citation: J Gen Virol. 2001 May;82(Pt 5):995-999
references.pmid: 11297674
references.citation: Virology. 1992 May;188(1):168-74
references.pmid: 1373552