title: Intracellular production of recombinant serpins in yeast.
abstract: Yeast are a valuable system for recombinant serpin production due to their ability to synthesize large amounts of heterologous gene products as well as their expression of folding chaperones and lack of endogenous serpin genes. In this chapter, we describe a method for intracellular expression of cytoplasmic serpins in the yeast Pichia pastoris. We also give details on how this system can be exploited to produce polymer-forming mutants of secretory serpins.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia.
authors.forename: Dion
authors.initials: D
authors.lastname: Kaiserman
authors.forename: Corinne
authors.initials: C
authors.lastname: Hitchen
authors.forename: Vita
authors.initials: V
authors.lastname: Levina
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Bottomley
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Bird
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D003470:Culture Media; D003720:Densitometry; D015870:Gene Expression; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D017510:Protein Folding; D021382:Protein Sorting Signals / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D015843:Serpins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D014170:Transformation, Genetic
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D015843:Serpins