title: [A new method of producing biologically active nanocomplexes by non-covalent conjugation of proteins with viral particles].
abstract: Currently, a range of biologically active molecules have been attached to plant and bacterial viras nanoscaffolds, yielding stable nanoparticles that display multiple copies of the desired molecule. In this paper we propose a new method of non-covalent attachment of peptides to the surface of virios. We have demonstrated that this method is efficient in a model system that includes tobacco mosaic virus particles, synthetic polycation (quaternized poly(4-vinylpyridine) carrying ethyl ethyl pendant radicals) and polypeptide of interest. This principle of step-by-step binding to the surface of virions was used for electrostatic association with hydrophilic fragment of influenza virus haemagglutinin.
authors.forename: A A
authors.initials: AA
authors.lastname: Iaroslavov
authors.forename: I V
authors.initials: IV
authors.lastname: Kaplan
authors.forename: T N
authors.initials: TN
authors.lastname: Erokhina
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Morozov
authors.forename: A G
authors.initials: AG
authors.lastname: Solov'ev
authors.forename: A D
authors.initials: AD
authors.lastname: Leshchiner
authors.forename: A A
authors.initials: AA
authors.lastname: Riakhnianskaia
authors.forename: A C
authors.initials: AC
authors.lastname: Malinin
authors.forename: L A
authors.initials: LA
authors.lastname: Stepanova
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
authors.forename: I G
authors.initials: IG
authors.lastname: Atabekov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D009975:Orthomyxoviridae / Q000737:chemistry* / Q000276:immunology; D011073:Polyamines / Q000138:chemical synthesis; D000071228:Polyelectrolytes; D011145:Polyvinyls / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry; D014027:Tobacco Mosaic Virus / Q000737:chemistry; D014771:Virion / Q000737:chemistry*
chemical_list: D006388:Hemagglutinins; D055767:Immobilized Proteins; D011073:Polyamines; D000071228:Polyelectrolytes; D011145:Polyvinyls; D011726:Pyridinium Compounds; C086116:poly(N-ethyl-4-vinylpyridine bromide)