title: Naturally occurring fragments from two distinct regions of the prostatic acid phosphatase form amyloidogenic enhancers of HIV infection.
abstract: Semen is the major vector for HIV-1 transmission. We previously isolated C-proximal fragments of the prostatic acid phosphatase (PAP) from semen which formed amyloid fibrils that potently enhanced HIV infection. Here, we used the same methodology and identified another amyloidogenic peptide. Surprisingly, this peptide is derived from an N-proximal fragment of PAP (PAP85-120) and forms, similar to the C-proximal fragments, positively charged fibrillar structures that increase virion attachment to cells. Our results provide a first example for amyloid formation by fragments of distinct regions of the same precursor and further emphasize the possible importance of amyloidogenic peptides in HIV transmission.
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Arnold
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Schnell
authors.forename: Onofrio
authors.initials: O
authors.lastname: Zirafi
authors.forename: Christina
authors.initials: C
authors.lastname: Stürzel
authors.forename: Christoph
authors.initials: C
authors.lastname: Meier
authors.forename: Tanja
authors.initials: T
authors.lastname: Weil
authors.forename: Ludger
authors.initials: L
authors.lastname: Ständker
authors.forename: Wolf-Georg
authors.initials: WG
authors.lastname: Forssmann
authors.forename: Nadia R
authors.initials: NR
authors.lastname: Roan
authors.forename: Warner C
authors.initials: WC
authors.lastname: Greene
authors.forename: Frank
authors.initials: F
authors.lastname: Kirchhoff
authors.forename: Jan
authors.initials: J
authors.lastname: Münch
mesh_terms: D000135:Acid Phosphatase; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D015658:HIV Infections / Q000201:enzymology* / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012661:Semen / Q000737:chemistry / Q000201:enzymology*; D016415:Sequence Alignment; D053585:Virus Attachment
chemical_list: D000682:Amyloid; D010446:Peptide Fragments; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases
references.citation: J Biol Chem. 2010 Nov 12;285(46):35488-96
references.pmid: 20833717
references.citation: Nature. 2011 Jun 15;475(7354):96-100
references.pmid: 21677644
references.citation: Nat Rev Immunol. 2005 Oct;5(10):783-92
references.pmid: 16200081
references.citation: Biophys J. 2005 Feb;88(2):1344-53
references.pmid: 15574704
references.citation: Cell. 2007 Dec 14;131(6):1059-71
references.pmid: 18083097
references.citation: Retrovirology. 2010 Jun 23;7:55
references.pmid: 20573198
references.citation: J Biol Chem. 2010 Jan 15;285(3):1861-9
references.pmid: 19897482
references.citation: J Immunol. 2010 Dec 15;185(12):7596-604
references.pmid: 21059891
references.citation: Proc Natl Acad Sci U S A. 2009 Jun 2;106(22):9033-8
references.pmid: 19451623
references.citation: Nat Biotechnol. 2002 Nov;20(11):1151-4
references.pmid: 12355096
references.citation: J Virol. 2002 Aug;76(16):8455-9
references.pmid: 12134048
references.citation: FASEB J. 2009 Oct;23(10):3609-18
references.pmid: 19487309
references.citation: Biophys J. 2003 Aug;85(2):1135-44
references.pmid: 12885658
references.citation: J Virol. 2009 Jan;83(1):73-80
references.pmid: 18945786
references.citation: Cell Host Microbe. 2011 Dec 15;10(6):541-50
references.pmid: 22177559