title: An integrated genetic linkage map of cultivated peanut (Arachis hypogaea L.) constructed from two RIL populations.
abstract: Construction and improvement of a genetic map for peanut (Arachis hypogaea L.) continues to be an important task in order to facilitate quantitative trait locus (QTL) analysis and the development of tools for marker-assisted breeding. The objective of this study was to develop a comparative integrated map from two cultivated × cultivated recombinant inbred line (RIL) mapping populations and to apply in mapping Tomato spotted wilt virus (TSWV) resistance trait in peanut. A total of 4,576 simple sequence repeat (SSR) markers from three sources: published SSR markers, newly developed SSR markers from expressed sequence tags (EST) and from bacterial artificial chromosome end-sequences were used for screening polymorphisms. Two cleaved amplified polymorphic sequence markers were also included to differentiate ahFAD2A alleles and ahFAD2B alleles. A total of 324 markers were anchored on this integrated map covering 1,352.1 cM with 21 linkage groups (LGs). Combining information from duplicated loci between LGs and comparing with published diploid maps, seven homoeologous groups were defined and 17 LGs (A1-A10, B1-B4, B7, B8, and B9) were aligned to corresponding A-subgenome or B-subgenome of diploid progenitors. One reciprocal translocation was confirmed in the tetraploid-cultivated peanut genome. Several chromosomal rearrangements were observed by comparing with published cultivated peanut maps. High consistency with cultivated peanut maps derived from different populations may support this integrated map as a reliable reference map for peanut whole genome sequencing assembling. Further two major QTLs for TSWV resistance were identified for each RILs, which illustrated the application of this map.
authors.affiliation: Department of Plant Pathology, University of Georgia, Tifton, GA, USA.
authors.forename: Hongde
authors.initials: H
authors.lastname: Qin
authors.forename: Suping
authors.initials: S
authors.lastname: Feng
authors.forename: Charles
authors.initials: C
authors.lastname: Chen
authors.forename: Yufang
authors.initials: Y
authors.lastname: Guo
authors.forename: Steven
authors.initials: S
authors.lastname: Knapp
authors.forename: Albert
authors.initials: A
authors.lastname: Culbreath
authors.forename: Guohao
authors.initials: G
authors.lastname: He
authors.forename: Ming Li
authors.initials: ML
authors.lastname: Wang
authors.forename: Xinyou
authors.initials: X
authors.lastname: Zhang
authors.forename: C Corley
authors.initials: CC
authors.lastname: Holbrook
authors.forename: Peggy
authors.initials: P
authors.lastname: Ozias-Akins
authors.forename: Baozhu
authors.initials: B
authors.lastname: Guo
mesh_terms: D010367:Arachis / Q000235:genetics* / Q000276:immunology / Q000821:virology; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D008040:Genetic Linkage / Q000235:genetics*; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D017865:Tospovirus / Q000276:immunology / Q000472:pathogenicity*
chemical_list: D018744:DNA, Plant
references.citation: BMC Plant Biol. 2010 Jan 27;10:17
references.pmid: 20105299
references.citation: Genetics. 2004 Jan;166(1):389-417
references.pmid: 15020432
references.citation: Theor Appl Genet. 2005 Oct;111(6):1060-71
references.pmid: 16088397
references.citation: Int J Plant Genomics. 2009;2009:715605
references.pmid: 19584933
references.citation: BMC Plant Biol. 2007 Feb 15;7:7
references.pmid: 17302987
references.citation: BMC Plant Biol. 2009 Aug 22;9:112
references.pmid: 19698131
references.citation: BMC Dev Biol. 2008 Feb 04;8:12
references.pmid: 18248674
references.citation: Mol Gen Genet. 2000 Jun;263(5):796-805
references.pmid: 10905347
references.citation: Genetics. 2001 Oct;159(2):823-37
references.pmid: 11606556
references.citation: Am J Bot. 2004 Sep;91(9):1294-303
references.pmid: 21652361
references.citation: BMC Plant Biol. 2004 Jul 14;4:11
references.pmid: 15253775
references.citation: BMC Plant Biol. 2009 Mar 24;9:35
references.pmid: 19309524
references.citation: Theor Appl Genet. 1993 Nov;87(3):379-84
references.pmid: 24190266
references.citation: Am J Bot. 2007 Dec;94(12):1963-71
references.pmid: 21636391
references.citation: Phytopathology. 2005 May;95(5):506-11
references.pmid: 18943315
references.citation: Nat Genet. 1995 Nov;11(3):241-7
references.pmid: 7581446
references.citation: Theor Appl Genet. 2008 Oct;117(6):883-94
references.pmid: 18604518
references.citation: Genome. 2006 Apr;49(4):336-45
references.pmid: 16699553
references.citation: BMC Plant Biol. 2009 Aug 03;9:103
references.pmid: 19650911
references.citation: Theor Appl Genet. 2004 Apr;108(6):1064-70
references.pmid: 15067392
references.citation: Theor Appl Genet. 2009 Feb;118(4):729-39
references.pmid: 19048225
references.citation: BMC Plant Biol. 2009 Apr 07;9:40
references.pmid: 19351409
references.citation: Genome. 2005 Feb;48(1):1-11
references.pmid: 15729391
references.citation: J Hered. 2002 Jan-Feb;93(1):77-8
references.pmid: 12011185