title: Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication.
abstract: A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the SureFood® PREP Animal system, and real-time PCR was carried out using SureFood® Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label.
authors.affiliation: A&T Food Laboratory, Mega Center No. 29 34045, Istanbul, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Demirhan
authors.forename: Pelin
authors.initials: P
authors.lastname: Ulca
authors.forename: Hamide Z
authors.initials: HZ
authors.lastname: Senyuva
mesh_terms: D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling / Q000379:methods*; D005780:Gelatin / Q000235:genetics; D005858:Germany; D008460:Meat / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*; D014421:Turkey
chemical_list: D017931:DNA Primers; D005780:Gelatin; D004247:DNA; D045303:Cytochromes b