title: Changes in the content of bioactive polyphenolic compounds of olive mill wastewater by the action of exogenous enzymes.
abstract: The aim behind the present research is to develop an enzymatic treatment for olive mill wastewater (OMW) to release high amounts of simple phenolics having high antioxidant value. OMW was hydrolyzed by a mixed enzyme preparation rich in β-glucosidase produced by  Aspergillus niger . This research shows that A. niger β-glucosidase played a major role in the release of simple phenolic compounds from OMW. These compounds were recovered by ethyl acetate extraction and identified by HPLC and LC-MS. The main identified phenolic compound is hydroxytyrosol. The results of enzymatic hydrolysis of OMW under optimum conditions indicated a maximum hydroxytyrosol concentration of 2.9 g L(-1) compared to 0.015 g L(-1) contained in the control (test without added enzyme). The above results prove that OMW is a potential substrate for producing hydroxytyrosol through enzymatic hydrolysis of its glycosides.
authors.affiliation: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional, AUF, Centre de Biotechnologie de Sfax, BP 1177, 3018 Sfax, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Hamza
authors.forename: Sonia
authors.initials: S
authors.lastname: Khoufi
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry*; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D012722:Sewage / Q000737:chemistry*; D014865:Waste Disposal, Fluid / Q000379:methods*; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D007220:Industrial Waste; D010636:Phenols; D012722:Sewage; C005975:3,4-dihydroxyphenylethanol; D001617:beta-Glucosidase; D010626:Phenylethyl Alcohol