title: rROP2(186-533): a novel peptide antigen for detection of IgM antibodies against Toxoplasma gondii.
abstract: Toxoplasma gondii infections are prevalent in a wide range of mammalian hosts including humans. Infection in pregnant women may cause the transmission of parasite to the fetus that makes serious problems. IgM antibodies against Toxoplasma (Toxo-IgM) have been believed to be significant indicators for both recently acquired and congenital toxoplasmosis. So far, however, there has not been any recognized protein of T. gondii that specifically reacts to IgM antibodies. Here, an antigen exclusively for detection of IgM antibodies screened by two-dimensional electrophoresis and mass spectrometry has been reported. The study identified 13 Toxoplasma proteins probed by IgG antibodies and one (rhpotry protein 2 [ROP2]) by IgM antibodies with human sera of Toxo-IgM(-)-IgG(+) and -IgM(+)-IgG(-), respectively, which had been prescreened by Toxo-IgM and -IgG commercial kits from the suspected cases. Following cloning, expression, and purification of the fragment of ROP2(186-533), an enzyme-linked immunosorbent assay with rROP2(186-533) to measure IgM and IgG antibodies was developed. As a result, 100%(48/48) of sera with Toxo-IgM(+)-IgG(-)showed positive Toxo-IgM but none of them (0%) showed positive Toxo-IgG when rROP2(186-533) was used as antigen. Neither Toxo-IgG nor Toxo-IgM antibodies were found when tested with 59 sera of Toxo-IgM(-)-IgG(+). These results indicate that rROP2(186-533) could be used as an antigen that specifically capture Toxo-IgM antibodies and may have a high potential in the serological diagnosis of both acute acquired and congenital toxoplasmosis.
authors.affiliation: Anhui Provincial Key Laboratory of Microbiology and Parasitology, Anhui Medical University, Hefei, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Yihong
authors.initials: Y
authors.lastname: Cai
authors.forename: Aimei
authors.initials: A
authors.lastname: Zhang
authors.forename: Xiucai
authors.initials: X
authors.lastname: Xu
authors.forename: Qingli
authors.initials: Q
authors.lastname: Luo
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Hu
authors.forename: Wenjian
authors.initials: W
authors.lastname: Song
authors.forename: Zhaorong
authors.initials: Z
authors.lastname: Lun
authors.forename: Fangli
authors.initials: F
authors.lastname: Lu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Jilong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008565:Membrane Proteins; D010455:Peptides; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis* / Q000276:immunology; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007075:Immunoglobulin M; D008565:Membrane Proteins; D010455:Peptides; D015800:Protozoan Proteins; C090555:ROP 2 protein, Toxoplasma gondii; D011994:Recombinant Proteins
references.citation: Clin Diagn Lab Immunol. 1998 Sep;5(5):627-31
references.pmid: 9729528
references.citation: J Food Prot. 2010 Jun;73(6):1121-3
references.pmid: 20537271
references.citation: J Clin Pathol. 1989 Dec;42(12):1285-90
references.pmid: 2613922
references.citation: J Clin Microbiol. 1997 Dec;35(12):3112-5
references.pmid: 9399504
references.citation: Mol Cell Proteomics. 2011 Jul;10(7):M110.006916
references.pmid: 21512035
references.citation: Acta Trop. 2010 Oct;116(1):1-8
references.pmid: 20451489
references.citation: J Clin Microbiol. 1997 Jan;35(1):174-8
references.pmid: 8968902
references.citation: Parasitol Res. 2011 Jan;108(1):151-60
references.pmid: 20859748
references.citation: Gynecol Obstet Invest. 1991;31(3):182-4
references.pmid: 2071060
references.citation: J Clin Microbiol. 1993 Jan;31(1):9-15
references.pmid: 8417037
references.citation: Infect Immun. 1996 Sep;64(9):3858-62
references.pmid: 8751939
references.citation: J Cell Biol. 1994 Nov;127(4):947-61
references.pmid: 7962077
references.citation: Lancet. 2004 Jun 12;363(9425):1965-76
references.pmid: 15194258