title: Molecular cloning of a new  wheat calreticulin gene TaCRT1 and expression analysis in plant defense  responses and abiotic stress resistance.
abstract: Calreticulin  proteins play essential roles in regulating various metabolic processes and in  molecular signal transduction in animals and plants. Using homologous PCR, we  screened a cDNA library of the wheat resistance gene Yr5 from a near-isogenic  line in the susceptible common wheat variety Taichung 29, which was inoculated  with an incompatible race CYR32 of Puccinia striiformis. We isolated a novel  full-length cDNA encoding calreticulin protein, which we named TaCRT1. Sequence  analyses indicated that TaCRT1 contains an open reading frame of 1287 bp in  length; it was deduced to encode 428 amino acids. Clustering analysis showed  that TaCRT1 belongs to group III of the calreticulin protein family.  Semi-quantitative RT-PCR was used to analyze expression profiles of the  isolated gene under biotic and abiotic stresses. Expression of TaCRT1 was  suppressed by exogenous application of phytohormones, such as abscisic acid and  methyl jasmonate, and by dehydration; but it was induced by CYR32 infection and  cold treatment. Based on the expression patterns, we propose that TaCRT1 participates  in regulatory processes involved in defense responses and stress resistance in  wheat.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: An
authors.forename: R M
authors.initials: RM
authors.lastname: Lin
authors.forename: F T
authors.initials: FT
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Feng
authors.forename: Y F
authors.initials: YF
authors.lastname: Xu
authors.forename: S C
authors.initials: SC
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D016000:Cluster Analysis; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics* / Q000276:immunology; D014908:Triticum / Q000235:genetics* / Q000276:immunology*
chemical_list: D037282:Calreticulin; D010940:Plant Proteins