title: Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2).
abstract: The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Yongin, South Korea.
authors.forename: Bolormaa
authors.initials: B
authors.lastname: Enkhbaatar
authors.forename: Uyangaa
authors.initials: U
authors.lastname: Temuujin
authors.forename: Ju-Hyeon
authors.initials: JH
authors.lastname: Lim
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Chi
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Chang
authors.forename: Soon-Kwang
authors.initials: SK
authors.lastname: Hong
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006867:Hydrolases; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014990:Xylans / Q000378:metabolism
chemical_list: D005936:Glucans; D011994:Recombinant Proteins; D014990:Xylans; C029353:xyloglucan; D006867:Hydrolases; D006026:Glycoside Hydrolases; C072818:xyloglucan endo(1-4)-beta-D-glucanase
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