title: [Establishment and evaluation of a real-time IAC-PCR for the detection of Salmonella].
abstract: OBJECTIVE
  The aim of this study was to establish a new EvaGreen real-time IAC-PCR for the rapid detection of Salmonella.
  
  
  METHODS
  We used Salmonella genomic comparison analysis to mine Salmonella-specific targets, and Primer Premier 5.0 to design primers which were evaluated by specificity and sensitivity tests.
  
  
  RESULTS
  We obtained a Salmonella-specific gene that encodes putative type III secretion protein (ssaQ), and specific primers (SsaQ6L/SsaQ6R) were designed based on this gene. Then we established IAC-PCR and EvaGreen real-time IAC-PCR assays, which showed 100% inclusivity and 100% exclusivity on all strains tested. Their detection limits of purified Salmonella genomic DNA were 14.9 copies/PCR and 2.76 copies/PCR respectively. Artificial contamination assays showed that Salmonella could be detected after 10 hours and 8 hours enrichment when the original bacterial concentration was 4.2 cfu/10 mL.
  
  
  CONCLUSIONS
  A new EvaGreen real-time IAC-PCR with high specificity and sensitivity was successfully developed for the rapid detection of Salmonella.
authors.affiliation: Joint Sino-US Food Safety Research Center and Bor Luh Food Safety Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China. danielxl2010@gmail.com
authors.forename: Xianlong
authors.initials: X
authors.lastname: Dan
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Lida
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.forename: Chunlei
authors.initials: C
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
mesh_terms: D005516:Food Microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity