title: Formaldehyde catabolism is essential in cells deficient for the Fanconi anemia DNA-repair pathway.
abstract: Metabolism is predicted to generate formaldehyde, a toxic, simple, reactive aldehyde that can damage DNA. Here we report a synthetic lethal interaction in avian cells between ADH5, encoding the main formaldehyde-detoxifying enzyme, and the Fanconi anemia (FA) DNA-repair pathway. These results define a fundamental role for the combined action of formaldehyde catabolism and DNA cross-link repair in vertebrate cell survival.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Rosado
authors.forename: Frédéric
authors.initials: F
authors.lastname: Langevin
authors.forename: Gerry P
authors.initials: GP
authors.lastname: Crossan
authors.forename: Minoru
authors.initials: M
authors.lastname: Takata
authors.forename: Ketan J
authors.initials: KJ
authors.lastname: Patel
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000235:genetics; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics / Q000378:metabolism*; D052218:Fanconi Anemia Complementation Group C Protein / Q000235:genetics / Q000502:physiology; D052240:Fanconi Anemia Complementation Group L Protein / Q000235:genetics / Q000502:physiology; D005557:Formaldehyde / Q000378:metabolism*; D055786:Gene Knockout Techniques; D053858:Metabolic Networks and Pathways
chemical_list: D052218:Fanconi Anemia Complementation Group C Protein; D005557:Formaldehyde; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D052240:Fanconi Anemia Complementation Group L Protein
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