title: Ribosome display for the selection of Sac7d scaffolds.
abstract: Combinatorial libraries of Sac7d have proved to be a valuable source of proteins with favorable biophysical properties and novel ligand specificities, so-called Nanofitins. Thus, Sac7d represents a promising scaffold alternative to antibodies for biotechnological and potentially clinical applications. We describe here the methodology for the construction of a library of Sac7d and its use for selection by ribosome display.
authors.affiliation: Unité de Biotechnologie, Biocatalyse et Biorégulation, UMR6204 CNRS, Université de Nantes, Nantes, France.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mouratou
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Béhar
authors.forename: Lauranne
authors.initials: L
authors.lastname: Paillard-Laurance
authors.forename: Stéphane
authors.initials: S
authors.lastname: Colinet
authors.forename: Frédéric
authors.initials: F
authors.lastname: Pecorari
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D019020:Directed Molecular Evolution / Q000379:methods*; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; D019151:Peptide Library; C095116:Sac7 protein, Sulfolobus