title: Spectroscopic investigation on the interaction of Cr(VI) with bovine serum albumin.
abstract: The interaction of potassium dichromate (Cr(VI)) with bovine serum albumin (BSA) was investigated by fluorescence, synchronous fluorescence, resonance light scattering (RLS), ultraviolet-visible absorption, and circular dichroism (CD) spectroscopies under simulated physiological conditions. The experimental results showed that Cr(VI) could quench the intrinsic fluorescence of BSA following a static quenching process, which indicates the formation of a Cr(VI)-BSA complex. The binding constant (KA) and binding site (n) were measured at different temperatures. The spectroscopic results also revealed that the binding of Cr(VI) to BSA can lead to the loosening of the protein conformation and can change the microenvironment and skeleton of BSA.
authors.affiliation: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, Jinan 250100, People's Republic of China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
authors.forename: Yuening
authors.initials: Y
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: Wansong
authors.initials: W
authors.lastname: Zong
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D008027:Light; D010316:Particle Size; D011192:Potassium Dichromate / Q000737:chemistry*; D011485:Protein Binding; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012710:Serum Albumin, Bovine; D011192:Potassium Dichromate