title: Potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, Burkholderia phytofirmans strain PsJN.
abstract: Burkholderia phytofirmans strain PsJN is a highly effective plant-beneficial endophyte. We have used a combination of capillary electrophoresis and methylation-sensitive amplification length polymorphism (CE-MSAP) analysis to investigate the potato genomic DNA cytosine methylation changes that occur in response to PsJN bacterization. Six weeks after PsJN inoculation, over 6800 loci were identified and assessed in two in vitro grown potato varieties, the strongly-responsive Red Pontiac and the poorly-responsive Superior. Compared to non-bacterized control, bacterized Red Pontiac exhibited little change in the overall cytosine methylation, although methylation polymorphisms did occur. In contrast, poorly-responsive Superior exhibited significantly higher levels of overall cytosine methylation and a decrease in the number of non-methylated sites in the bacterized plants compared to controls. Superior had significantly higher DNA methylation and DNA hyper-methylation than Red Pontiac, suggesting that enhanced DNA loci methylation is involved in the suppression of PsJN-induced plant growth stimulation. Several DNA fragments, corresponding to different open reading frames exhibiting methylation polymorphisms in Red Pontiac or Superior were sequenced. Gene expression analysis of a subset of those genes was carried out using real time PCR. We identified several genes whose transcript levels were either enhanced or decreased in response to PsJN in a variety-specific way, as well as genes that were specifically enhanced in both varieties in response to the endophyte.
authors.affiliation: Institute for Sustainable and Renewable Resources, Institute for Advanced Learning and Research, 150 Slayton Avenue, Danville, VA 24540, USA.
authors.forename: Kedong
authors.initials: K
authors.lastname: Da
authors.forename: Jerzy
authors.initials: J
authors.lastname: Nowak
authors.forename: Barry
authors.initials: B
authors.lastname: Flinn
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D019117:Burkholderia; D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation; D004586:Electrophoresis; D060026:Endophytes; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D016366:Open Reading Frames; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D013045:Species Specificity
chemical_list: D012333:RNA, Messenger; D003596:Cytosine