title: Heterogeneity in the ITS of the ribosomal DNA of Pyrenophora graminea isolates differing in xylanase and amylase production.
abstract: Xylanase and amylase have gained increasing interest because of their various biotechnology applications. In this research, the restriction of PCR-amplified internal transcribed spacers (ITS) of ribosomal DNA (rDNA) was used to confirm the genetic variation among 22 isolates of Pyrenophora graminea differing in their xylanase and amylase production. The fingerprints generated from the six restriction digestions of the rDNA ITS region showed high levels ofintraspecific variation within the P. graminea population. Neighbour-Joining diagram, based on Nei's genetic distances, showed that isolates formed two phylogenetic groups. No apparent association could be observed between xylanase and amylase production and genetic diversity among the twenty-two isolates.
authors.affiliation: Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria, P.O. Box 609.
authors.forename: Y
authors.initials: Y
authors.lastname: Bakri
authors.forename: M I E
authors.initials: MI
authors.lastname: Arabi
authors.forename: M
authors.initials: M
authors.lastname: Jawhar
mesh_terms: D000681:Amylases / Q000096:biosynthesis*; D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004275:DNA, Ribosomal / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D056726:Genetic Association Studies; D014644:Genetic Variation; D010802:Phylogeny
chemical_list: D004275:DNA, Ribosomal; D004262:DNA Restriction Enzymes; D000681:Amylases; D043364:Endo-1,4-beta Xylanases