title: [Association of IL-8 gene polymorphisms with inflammatory bowel disease in Chinese patients].
abstract: OBJECTIVE
  To investigate the association of interleukin 8 (IL-8) gene polymorphisms with the risks of inflammatory bowel disease (IBD).
  
  
  METHODS
  Single nucleotide polymorphisms (SNPs) of IL-8 gene at -845 T/C, -738 T/A, -353 A/T, -251 T/A and +678 T/C were analyzed in 183 IBD patients. They included Crohn's disease (CD, n = 41), ulcerative colitis (UC, n = 142) and healthy controls (n = 160). The methods of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-sequence specific primers (PCR-SSP) were employed.
  
  
  RESULTS
  No association was observed between any of these five SNPs in IL-8 gene with the occurrence of IBD. A specific haplotype AAT (-353 A/T, -251 T/A & +678 T/C) was over-represented in UC cases when compared with controls (31.0% vs 23.7%, P = 0.046). But the distributions of this haplotype did not show significant difference between CD cases and controls.
  
  
  CONCLUSIONS
  Our data support a significant but modest association between the AAT haplotype of IL-8 gene and UC (OR = 1.441, 95%CI 1.007 - 2.063).
authors.affiliation: School of Life Sciences, Wenzhou Medical College, Zhejiang, China.
authors.forename: Wan-dong
authors.initials: WD
authors.lastname: Liang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Li
authors.forename: Ke-shen
authors.initials: KS
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Yang
authors.forename: Yun-tian
authors.initials: YT
authors.lastname: Bi
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000453:epidemiology / Q000235:genetics*; D016209:Interleukin-8 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8