title: Toward the quantification of the 13CO2/12CO2 ratio in exhaled mouse breath with mid-infrared hollow waveguide gas sensors.
abstract: Mouse sepsis models are used to gain insight into the complex processes involved with patients suffering from glucose metabolism disorders. Measuring the expiratory release of (13)CO(2) after administering stable labeled (13)C(6)-glucose enables assessment of the in vivo integrity and functionality of key metabolic processes. In the present study, we demonstrate that Fourier transform infrared spectroscopy operating in the mid-infrared spectral regime (2-20 μm) combined with hollow waveguide gas sensing modules simultaneously serving as a miniaturized gas cell and as a waveguide are capable of quantitatively monitoring (13)CO(2) enrichment levels in low volume mouse breath samples.
authors.affiliation: Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Ulm, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wilk
authors.forename: Felicia
authors.initials: F
authors.lastname: Seichter
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Erhan
authors.initials: E
authors.lastname: Tütüncü
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
authors.forename: Josef A
authors.initials: JA
authors.lastname: Vogt
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wachter
authors.forename: Peter
authors.initials: P
authors.lastname: Radermacher
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001944:Breath Tests / Q000295:instrumentation / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis* / Q000378:metabolism; D045853:Exhalation; D005947:Glucose / Q000378:metabolism; D044882:Glucose Metabolism Disorders / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*
chemical_list: D002245:Carbon Dioxide; D005947:Glucose