title: Proteomic approach in the identification of fertility pattern in seminal plasma of fertile men.
abstract: OBJECTIVE
  To identify a panel of common seminal proteins in human seminal plasma by fertile men that might be involved in successful reproduction.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Five fertile men who conceived within 3 months before the start of the study.
  
  
  METHODS
  None.
  
  
  METHODS
  Proteomic analysis performed by an Ultimate 3000 Nano/Micro-HPLC apparatus equipped with an FLM-3000-Flow manager module and coupled with an LTQ Orbitrap XL hybrid mass spectrometer; gene ontology analysis.
  
  
  RESULTS
  From 919 to 1,487 unique proteins were identified per individual subject sample. Among these proteins, 83 proteins were present in all samples, including some proteins that might be involved in male fertility, such as semenogelin I, semenogelin II, olfactory receptor 5R1, lactoferrin, hCAP18, spindling, and clusterin. The gene ontology annotation analysis provided further information in describing common pattern in male fertility.
  
  
  CONCLUSIONS
  The identification of common seminal plasma proteome in fertile men could provide better insight into the physiology of male fertility and might identify novel markers of male infertility.
authors.affiliation: International Scientific Institute Paolo VI, Università Cattolica del S. Cuore, Rome, Italy. milardid@yahoo.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Milardi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Grande
authors.forename: Federica
authors.initials: F
authors.lastname: Vincenzoni
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
authors.forename: Alfredo
authors.initials: A
authors.lastname: Pontecorvi
authors.forename: Laura
authors.initials: L
authors.lastname: De Marinis
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Riccardo
authors.initials: R
authors.lastname: Marana
mesh_terms: D015415:Biomarkers; D005260:Female; D005298:Fertility / Q000502:physiology*; D012739:Gonadal Steroid Hormones / Q000097:blood; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000503:physiopathology*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D040901:Proteomics / Q000379:methods* / Q000592:standards*; D012016:Reference Values; D012661:Semen / Q000502:physiology*
chemical_list: D015415:Biomarkers; D012739:Gonadal Steroid Hormones