title: Label-free fluorescence method for screening G-quadruplex ligands.
abstract: G-quadruplex ligands can interfere with the telomere structure, telomere elongation/replication, and proliferation of cancer cells. A key element in the development of potent G-quadruplex ligands is the screening of large chemical libraries of potential candidates. Here, we describe a simple fluorescence method for screening of G-quadruplex ligands. The method is based on the ability of G-quadruplex ligands to displace hemin from G-quadruplex-based DNAzyme, resulting in a decrease of its catalytic activity on the fluorescence-developing reaction between p-hydroxyphenylacetic acid and H(2)O(2). The method eliminates the requirement for expensive and time-consuming preparation of labeled DNA. Our method provides a simple, cheap, and sensitive approach to screen G-quadruplex ligands (potential antitumor drugs).
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Fu
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
authors.forename: Yuanfu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D021881:DNA, Catalytic; D005453:Fluorescence; D054856:G-Quadruplexes; D006861:Hydrogen Peroxide; D008024:Ligands; D010648:Phenylacetates; D013050:Spectrometry, Fluorescence / Q000379:methods*; D016615:Telomere
chemical_list: D021881:DNA, Catalytic; D008024:Ligands; D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D006861:Hydrogen Peroxide