title: Differential effects of alpha-particle radiation and x-irradiation on genes associated with apoptosis.
abstract: This study examined differential effects of alpha-(α-) particle radiation and X-rays on apoptosis and associated changes in gene expression. Human monocytic cells were exposed to α-particle radiation and X-rays from 0 to 1.5 Gy. Four days postexposure, cell death was measured by flow cytometry and 84 genes related to apoptosis were analyzed using real-time PCR. On average, 33% of the cells were apoptotic at 1.5 Gy of α-particle radiation. Transcript profiling showed statistical expression of 15 genes at all three doses tested. Cells exposed to X-rays were <5% apoptotic at ~1.5 Gy and induced less than a 2-fold expression in 6 apoptotic genes at the higher doses of radiation. Among these 6 genes, Fas and TNF-α were common to the α-irradiated cells. This data suggests that α-particle radiation initiates cell death by TNF-α and Fas activation and through intermediate signalling mediators that are distinct from X-irradiated cells.
authors.affiliation: Consumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, Canada K1A 0K9.
authors.forename: Vinita
authors.initials: V
authors.lastname: Chauhan
authors.forename: Matthew
authors.initials: M
authors.lastname: Howland
authors.forename: Jeremy
authors.initials: J
authors.lastname: Chen
authors.forename: Barbara
authors.initials: B
authors.lastname: Kutzner
authors.forename: Ruth C
authors.initials: RC
authors.lastname: Wilkins
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