title: Induction of oxidative stress and inhibition of superoxide dismutase expression in rat cerebral cortex and cerebellum by PTU-induced hypothyroidism and its reversal by curcumin.
abstract: The present study was carried out to elucidate the effectiveness of curcumin in ameliorating the expression of superoxide dismutase (SOD) in cerebral cortex and cerebellum of rat brain under 6-propyl-2-thiouracil (PTU)-induced hypothyroidism. Induction of hypothyroidism in adult rats by PTU resulted in augmentation of lipid peroxidation (LPx), an index of oxidative stress in cerebellum but not in cerebral cortex. Curcumin-supplementation to PTU-treated (hypothyroid) rats showed significant reduction in the level of LPx in both the regions of brain. The decreased translated products (SOD1 and SOD2) and the unchanged activity of SOD in cerebral cortex of PTU-treated rats were increased on supplementation of curcumin to the hypothyroid rats. Declined translated products of SOD1 and SOD2 in cerebellum of PTU-treated rats were alleviated on administration of curcumin to hypothyroid rats. On the other hand, the decreased activity of SOD in cerebellum of PTU-treated rats was further declined on administration of curcumin to the hypothyroid rats. Results of the present investigation indicate that curcumin differentially modulates the expression of superoxide dismutase in rat brain cortex and cerebellum under PTU-induced hypothyroidism.
authors.affiliation: Department of Biotechnology, Utkal University, Bhubaneswar, 751004 Odisha, India. jenasrikanta@yahoo.co.in
authors.forename: Srikanta
authors.initials: S
authors.lastname: Jena
authors.forename: Chinmay
authors.initials: C
authors.lastname: Anand
authors.forename: Gagan Bihari Nityananda
authors.initials: GB
authors.lastname: Chainy
authors.forename: Jagneshwar
authors.initials: J
authors.lastname: Dandapat
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D013956:Antithyroid Agents / Q000633:toxicity; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D003474:Curcumin / Q000627:therapeutic use*; D004195:Disease Models, Animal; D007037:Hypothyroidism / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011441:Propylthiouracil / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013956:Antithyroid Agents; D003474:Curcumin; D011441:Propylthiouracil; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D017392:Thiobarbituric Acid Reactive Substances; D000099156:Superoxide Dismutase 2; C000606292:Sod1 protein, rat
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