title: Identification of splice variants, targeted microRNAs and functional single nucleotide polymorphisms of the BOLA-DQA2 gene in dairy cattle.
abstract: Major histocompatibility complex, class II, DQ alpha 2, also named BOLA-DQA2, belongs to the Bovine Leukocyte Antigen (BOLA) class II genes which are involved in the immune response. To explore the variability of the BOLA-DQA2 gene and resistance to mastitis in cows, the splice variants (SV), targeted microRNAs (miRNAs), and single nucleotide polymorphisms (SNPs) were identified in this study. A new SV (BOLA-DQA2-SV1) lacking part of exon 3 (195 bp) and two 3'-untranslated regions (UTR) (52 bp+167 bp) of the BOLA-DQA2 gene was found in the healthy and mastitis-infected mammary gland tissues. Four of 13 new SNPs and multiple nucleotide polymorphisms resulted in amino acid changes in the protein and SNP (c. +1283 C>T) may affect the binding to the seed sequence of bta-miR-2318. Further, we detected the relative expressions of two BOLA-DQA2 transcripts and five candidated microRNAs binding to the 3'-UTR of two transcripts in the mammary gland tissues in dairy cattle by using the quantitative real-time polymerase chain reaction. The result showed that expression of the BOLA-DQA2-SV1 mRNA was significantly upregulated 2.67-fold (p<0.05) in mastitis-infected mammary tissues (n = 5) compared with the healthy mammary gland mammary tissues (n = 5). Except for bta-miR-1777a, miRNA expression (bta-miR-296, miR-2430, and miR-671) was upregulated 1.75 to 2.59-fold (p<0.05), whereas miR-2318 was downregulated in the mastitis cows. Our findings reveal that BOLA-DQA2-SV1 may play an important role in the mastitis resistance in dairy cattle. Whether the SNPs affect the structure of the BOLA-DQA2 gene or association with mastitis resistance is unknown and warrants further investigation.
authors.affiliation: Laboratory of Molecular Genetics and Breeding, Center of Dairy Cattle Research, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Qinlei
authors.initials: Q
authors.lastname: Hou
authors.forename: Jinming
authors.initials: J
authors.lastname: Huang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ju
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Li
authors.forename: Liming
authors.initials: L
authors.lastname: Li
authors.forename: Changfa
authors.initials: C
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Minghai
authors.initials: M
authors.lastname: Hou
authors.forename: Suqin
authors.initials: S
authors.lastname: Hang
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhong
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000473:pathology; D008414:Mastitis, Bovine / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D020413:3' Untranslated Regions; C098096:BoLA-DQA antigen; D000949:Histocompatibility Antigens Class II; D035683:MicroRNAs; D012333:RNA, Messenger
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