title: Photoligation of self-assembled DNA constructs containing anthracene-functionalized 2'-amino-LNA monomers.
abstract: Efficient synthesis of a novel anthracene-functionalized 2'-amino-LNA phosphoramidite derivative is described together with its incorporation into oligodeoxynucleotides. Two DNA strands with the novel 2'-N-anthracenylmethyl-2'-amino-LNA monomers can be effectively cross-linked by photoligation at 366 nm in various types of DNA constructs. Successful application of three differently functionalized 2'-amino-LNA monomers in self-assembled higher ordered structures for simultaneous cross-linking and monitoring of assembly formation is furthermore demonstrated.
authors.affiliation: Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Karol
authors.initials: K
authors.lastname: Pasternak
authors.forename: Anna
authors.initials: A
authors.lastname: Pasternak
authors.forename: Pankaj
authors.initials: P
authors.lastname: Gupta
authors.forename: Rakesh N
authors.initials: RN
authors.lastname: Veedu
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D000873:Anthracenes / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D055668:Photochemical Processes
chemical_list: D000873:Anthracenes; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C527207:amino-LNA; C434331:phosphoramidite; D004247:DNA; C034020:anthracene