title: Prostatic acid phosphatase is expressed in human prostate cancer bone metastases and promotes osteoblast differentiation.
abstract: Prostate cancer (PCa) bone metastases are a major cause of morbidity and mortality. There are no effective therapies for PCa bone metastases that prolong survival. Prostatic acid phosphatase (PAP) is a secretory protein expressed by PCa cells. We demonstrate that PAP is strongly expressed in PCa bone metastases in 7/7 patients, while prostate-specific antigen (PSA) is only weakly expressed. The human PCa cell line VCaP secretes PAP and induces an osteoblastic reaction in bone similar to that seen in human PCa bone metastases. Coculture of MC3T3 mouse preosteoblast cells with VCaP cells induces MC3T3 cell growth and differentiation as measured by alkaline phosphatase secretion, and this effect is inhibited by addition of the PAP-inhibitor, l-tartrate. Taken together, these data indicate that PAP is expressed in PCa bone metastases and may play a causal role in the osteoblastic phase of the disease.
authors.affiliation: Department of Urology, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kirschenbaum
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Shen
authors.initials: S
authors.lastname: Yao
authors.forename: Amanda
authors.initials: A
authors.lastname: Leiter
authors.forename: Alice C
authors.initials: AC
authors.lastname: Levine
mesh_terms: D000135:Acid Phosphatase; D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000556:secondary*; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000201:enzymology* / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D017430:Prostate-Specific Antigen / Q000096:biosynthesis; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*
chemical_list: D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases; D017430:Prostate-Specific Antigen