title: Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip.
abstract: Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a "heart on a chip" that exploits muscular thin film technology--biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film--to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.
authors.affiliation: Disease Biophysics Group, Wyss Institute of Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard University, 29 Oxford St, Pierce Hall Rm 321, Cambridge, MA 02138, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Grosberg
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Alford
authors.forename: Megan L
authors.initials: ML
authors.lastname: McCain
authors.forename: Kevin Kit
authors.initials: KK
authors.lastname: Parker
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004837:Epinephrine / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering
chemical_list: D000180:Acrylic Resins; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; C052970:poly-N-isopropylacrylamide; C013830:baysilon; D004837:Epinephrine
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