title: [The study of anti-tumor activities of DC vaccine loaded with multi-epotipes of survivin].
abstract: OBJECTIVE
  To observe the anti-tumor activity of dendritic cell (DC)vaccine loaded with  multi-epitopes of survivin.
  
  
  METHODS
  The recombinant plasmid pPIRESneo3.0-survivin (4)/Th which include four survivin HLA-A2-restricted CD8(+); CTL epitopes and a CD4(+);Th epitope, pPIRESneo3.0-survivin (4) which include four survivin CD8(+); CTL epitopes, were transfected into human dendritic cells respectively. There were five groups,  which included survivin(4)/Th group,  survivin(4)group,  empty plasmid group,  untransfected group and T lymphocytes group The expression of CD83 and CD86 on the surface of DCs,  the expression of CD4 and CD8a on the surface of T lymphocytes,  the apoptotic rates of MCF-7 cells after treated by DC vaccine were measured by flow cytometry;  IFN-γ levels of all groups were detected by ELISA and the growth inhibition of MCF-7 cells after being treated with DC vaccine was tested by MTT colorimetry.
  
  
  RESULTS
  The results of flow cytometry revealed that high levels CD83 and CD86 were expressed on the surface of DCs;  high levels CD4 and CD8a were expressed on the surface of T lymphocytes;  the IFN-γ levels in survivin(4)/Th group [(66.50±3.34)ng/L]were significantly higher than that in survivin(4)group[(46.10±1.35)ng/L],  empty plasmid group[(25.17±0.32)ng/L],  untransfected group [(25.47±0.95)ng/L]  or T lymphocytes group[(23.73±0.50)ng/L](P<0.05).  The inhibition rate of MCF-7 cells in survivin(4)/Th group was significantly higher than that in survivin(4)group,  empty plasmid group,  untransfected group or T lymphocytes group(P<0.05). The apoptotic rate of MCF-7 cells in survivin(4)/Th group was (10.63±0.29)% after treated by DC vaccine,  which was significantly higher than that in in survivin(4)group,  empty plasmid group,  untransfected group or T lymphocytes group(P<0.05).
  
  
  CONCLUSIONS
  The DCs vaccine loaded with multi- CD8(+); CTL epitopes of survivin has strong anti-tumor effects. CD4(+); Th cells can promote the anti-tumor activity of CD8(+);CTL.
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, China. guomeng62@126.com
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.forename: Xiao-yuan
authors.initials: XY
authors.lastname: Wang
authors.forename: Wen-zhe
authors.initials: WZ
authors.lastname: Song
authors.forename: Xiang-nong
authors.initials: XN
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Zhi-long
authors.initials: ZL
authors.lastname: Huang
mesh_terms: D019496:Cancer Vaccines / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000276:immunology* / Q000378:metabolism; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D019496:Cancer Vaccines; D000939:Epitopes; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D007371:Interferon-gamma