title: Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3,4-dichloroisocoumarin.
abstract: A recombinant lipase (triacylglycerol acylhydrolase; EC 3.1.1.3) from the bacterium Streptomyces rimosus was inhibited by the serine protease inhibitor 3,4-dichloroisocoumarin and crystallized by the hanging-drop vapour-diffusion method at 291 K. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 38.1, b = 78.7, c = 56.6 Å, β = 104.5° and probably two molecules in the asymmetric unit. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation on the XRD beamline of the Elettra synchrotron, Trieste, Italy.
authors.affiliation: Department of Physical Chemistry, Rudjer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia.
authors.forename: Ivana Leščić
authors.initials: IL
authors.lastname: Ašler
authors.forename: Jasenka
authors.initials: J
authors.lastname: Pigac
authors.forename: Dušica
authors.initials: D
authors.lastname: Vujaklija
authors.forename: Marija
authors.initials: M
authors.lastname: Luić
authors.forename: Zoran
authors.initials: Z
authors.lastname: Štefanić
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005110:Extracellular Space / Q000201:enzymology; D049934:Isocoumarins; D008049:Lipase / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D003374:Coumarins; D004791:Enzyme Inhibitors; D049934:Isocoumarins; C046235:3,4-dichloroisocoumarin; D008049:Lipase
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