title: The characterization and comparison of Staphylococcus aureus by antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction, and random amplified polymorphic DNA-polymerase chain reaction.
abstract: Thirty-five of Staphylococcus aureus isolated from food raw materials and workers' hands in a noshery were characterized using antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA (RAPD)-PCR. As a similarity threshold of 90%, ERIC-PCR classified 35 S. aureus isolates into 28 ERIC types with discrimination indexes (D) of 0.984, while RAPD-PCR grouped 35 S. aureus isolates into five clusters (A-E) showing 19 RAPD types with D of 0.949. Four resistance patterns were observed with D of 0.826. A comparison of characterization of S. aureus indicated a clear correlation between ERIC-PCR and RAPD-PCR or resistance patterns in some strains. It was concluded that ERIC-PCR method could be used for genetic diversity of S. aureus and tracing the sources of it from the food chain.
authors.affiliation: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China. yeyw04@mails.gucas.ac.cn
authors.forename: Yingwang
authors.initials: Y
authors.lastname: Ye
authors.forename: Qihuan
authors.initials: Q
authors.lastname: Jiang
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wu
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005516:Food Microbiology; D014644:Genetic Variation; D006225:Hand / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012147:Restaurants; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial