title: New trends in fluorescence in situ hybridization for identification and functional analyses of microbes.
abstract: Fluorescence in situ hybridization (FISH) has become an indispensable tool for rapid and direct single-cell identification of microbes by detecting signature regions in their rRNA molecules. Recent advances in this field include new web-based tools for assisting probe design and optimization of experimental conditions, easy-to-implement signal amplification strategies, innovative multiplexing approaches, and the combination of FISH with transmission electron microscopy or extracellular staining techniques. Further emerging developments focus on sorting FISH-identified cells for subsequent single-cell genomics and on the direct detection of specific genes within single microbial cells by advanced FISH techniques employing various strategies for massive signal amplification.
authors.affiliation: Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria. wagner@microbial-ecology.net
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.forename: Susanne
authors.initials: S
authors.lastname: Haider
mesh_terms: D001105:Archaea / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000639:trends; D015345:Oligonucleotide Probes / Q000235:genetics; D015347:RNA Probes / Q000235:genetics
chemical_list: D017931:DNA Primers; D015345:Oligonucleotide Probes; D015347:RNA Probes