title: Programmed cell death in plants: protective effect of mitochondrial-targeted quinones.
abstract: Ubiquinone or plastoquinone covalently linked to synthetic decyltriphenylphosphonium (DTPP(+)) or rhodamine cations prevent programmed cell death (PCD) in pea leaf epidermis induced by chitosan or CN(-). PCD was monitored by recording the destruction of cell nuclei. CN(-) induced the destruction of nuclei in both epidermal cells (EC) and guard cells (GC), whereas chitosan destroyed nuclei in EC not in GC. The half-maximum concentrations for the protective effects of the quinone derivatives were within the pico- and nanomolar range. The protective effect of the quinones was removed by a protonophoric uncoupler and reduced by tetraphenylphosphonium cations. CN(-)-Induced PCD was accelerated by the tested quinone derivatives at concentrations above 10(-8)-10(-7) M. Unlike plastoquinone linked to the rhodamine cation (SkQR1), DTPP(+) derivatives of quinones suppressed menadione-induced H(2)O(2) generation in the cells. The CN(-)-induced destruction of GC nuclei was prevented by DTPP(+) derivatives in the dark not in the light. SkQR1 inhibited this process both in the dark and in the light, and its effect in the light was similar to that of rhodamine 6G. The data on the protective effect of cationic quinone derivatives indicate that mitochondria are involved in PCD in plants.
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Vasil'ev
authors.forename: E V
authors.initials: EV
authors.lastname: Dzyubinskaya
authors.forename: D B
authors.initials: DB
authors.lastname: Kiselevsky
authors.forename: A A
authors.initials: AA
authors.lastname: Shestak
authors.forename: V D
authors.initials: VD
authors.lastname: Samuilov
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D048271:Chitosan / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology*; D018532:Pisum sativum / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010971:Plastoquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: C534903:10-(6'-plastoquinonyl) decylrhodamine 19; C532038:10-(6'-plastoquinonyl)decyltriphenylphosphonium; D009943:Organophosphorus Compounds; D020011:Protective Agents; D012235:Rhodamines; C570713:methylplastoquinonyldecyltriphenylphosphonium; D014451:Ubiquinone; C429014:mitoquinone; D048271:Chitosan; D010971:Plastoquinone