title: Changing patterns of acute phase proteins and inflammatory mediators in experimental caprine coccidiosis.
abstract: This experiment was conducted to assess the changing patterns and relative values of acute phase proteins and inflammatory cytokines in experimental caprine coccidiosis. Eighteen newborn kids were allocated to 3 equal groups. Two groups, A and B, were inoculated with a single dose of 1×10(3) and1×10(5) sporulated oocysts of Eimeria arloingi, respectively. The third group, C, received distilled water as the control. Blood samples were collected from the jugular vein of each kid in both groups before inoculation and at days 7, 14, 21, 28, 35, and 42 post-inoculation (PI), and the levels of haptoglobin (Hp), serum amyloid A (SAA), TNF-α, and IFN-γ were measured. For histopathological examinations, 2 kids were selected from each group, euthanized, and necropsied on day 42 PI. Mean Hp concentrations in groups A and B (0.34 and 0.68 g/L) at day 7 PI were 3.2 and 6.3 times higher than the levels before inoculation. The mean SAA concentrations in groups A and B (25.6 and 83.5 µg/ml) at day 7 PI were 4.2 and 13.7 times higher than the levels before inoculation. The magnitude and duration of the Hp and SAA responses correlated well with the inoculation doses and the severity of the clinical signs and diarrhea in kids. These results were consistent with the histopathological features, which showed advanced widespread lesions in group B. In both groups, significant correlations were observed for TNF-α and IFN-γ with SAA and Hp, respectively. In conclusion, Hp and SAA can be useful non-specific diagnostic indicators in caprine coccidiosis.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 71345 Shiraz, Iran. hashemnia@shirazu.ac.ir
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemnia
authors.forename: Azizollah
authors.initials: A
authors.lastname: Khodakaram-Tafti
authors.forename: Seyed Mostafa
authors.initials: SM
authors.lastname: Razavi
authors.forename: Saeed
authors.initials: S
authors.lastname: Nazifi
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D001774:Blood Chemical Analysis; D003048:Coccidiosis / Q000276:immunology* / Q000473:pathology*; D004195:Disease Models, Animal; D004539:Eimeria / Q000472:pathogenicity*; D006041:Goats; D006651:Histocytochemistry; D018836:Inflammation Mediators / Q000032:analysis*
chemical_list: D000209:Acute-Phase Proteins; D018836:Inflammation Mediators
references.citation: Biochem J. 1990 Feb 1;265(3):621-36
references.pmid: 1689567
references.citation: Infect Immun. 1992 Sep;60(9):3921-4
references.pmid: 1500201
references.citation: Vet Rec. 1999 Apr 17;144(16):437-41
references.pmid: 10343375
references.citation: Vet Rec. 1991 Feb 16;128(7):147-9
references.pmid: 1903006
references.citation: Vet J. 1998 Jan;155(1):53-61
references.pmid: 9455159
references.citation: Vet J. 2004 Jul;168(1):28-40
references.pmid: 15158206
references.citation: J Vet Med B Infect Dis Vet Public Health. 2005 Dec;52(10):417-27
references.pmid: 16364016
references.citation: J Am Vet Med Assoc. 1991 Jun 1;198(11):1898-901
references.pmid: 1714890
references.citation: Vet Clin North Am Food Anim Pract. 2006 Nov;22(3):613-21
references.pmid: 17071356
references.citation: Immunol Today. 1994 Feb;15(2):74-80
references.pmid: 7512342
references.citation: J Rheumatol Suppl. 1994 Oct;42:9-13
references.pmid: 7529838
references.citation: Vet Rec. 2002 Feb 23;150(8):241-4
references.pmid: 11916025
references.citation: Int J Parasitol. 1998 Jan;28(1):165-79
references.pmid: 9504343
references.citation: Res Vet Sci. 1994 Jul;57(1):125-8
references.pmid: 7973086
references.citation: J Clin Immunol. 1999 Jul;19(4):203-14
references.pmid: 10471974
references.citation: Vet Parasitol. 1998 Jan 31;74(2-4):165-72
references.pmid: 9561704
references.citation: Vet Rec. 2002 Jul 20;151(3):85-9
references.pmid: 12164226
references.citation: Vet Immunol Immunopathol. 1996 Jun 1;51(3-4):277-92
references.pmid: 8792565
references.citation: Res Vet Sci. 1996 Nov;61(3):252-7
references.pmid: 8938857
references.citation: Res Vet Sci. 1994 Jul;57(1):129-32
references.pmid: 7973087
references.citation: Parasitology. 1986 Apr;92 ( Pt 2):279-89
references.pmid: 3714300
references.citation: Vet J. 2009 Nov;182(2):315-9
references.pmid: 18783967
references.citation: Res Vet Sci. 2000 Oct;69(2):153-8
references.pmid: 11020367
references.citation: J Infect Dis. 1998 Apr;177(4):1076-9
references.pmid: 9534986
references.citation: Ann Clin Biochem. 1993 Jul;30 ( Pt 4):373-6
references.pmid: 7691039
references.citation: Am J Vet Res. 1996 May;57(5):646-9
references.pmid: 8723875
references.citation: Parasitol Int. 2001 Mar;50(1):15-26
references.pmid: 11267928
references.citation: Acta Trop. 1992 Dec;52(2-3):121-8
references.pmid: 1283805
references.citation: Vet Q. 1995 Jun;17(2):41-50
references.pmid: 7571278
references.citation: Acta Vet Scand. 1999;40(1):35-46
references.pmid: 10418194
references.citation: J Dairy Res. 1996 Aug;63(3):351-60
references.pmid: 8864931
references.citation: Vet Rec. 2001 Jan 13;148(2):35-41
references.pmid: 11202551
references.citation: J Anim Sci. 1994 Feb;72(2):309-14
references.pmid: 8157515
references.citation: Vet Q. 1994 Mar;16(1):21-3
references.pmid: 8009814
references.citation: J Anim Sci. 1997 May;75(5):1244-55
references.pmid: 9159271
references.citation: J S Afr Vet Assoc. 2000 Mar;71(1):38-42
references.pmid: 10949516