title: Direct maldi-tof mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories.
abstract: We evaluated the reliability of the Bruker Daltonik's MALDI Biotyper system in species-level identification of yeasts directly from blood culture bottles. Identification results were concordant with those of the conventional culture-based method for 95.9% of Candida albicans (187/195) and 86.5% of non-albicans Candida species (128/148). Results were available in 30 min (median), suggesting that this approach is a reliable, time-saving tool for routine identification of Candida species causing bloodstream infection.
authors.affiliation: Istituto di Microbiologia,a Istituto di Clinica delle Malattie Infettive, Rome, Italy. tspanu@rm.unicatt.it
authors.forename: Teresa
authors.initials: T
authors.lastname: Spanu
authors.forename: Brunella
authors.initials: B
authors.lastname: Posteraro
authors.forename: Barbara
authors.initials: B
authors.lastname: Fiori
authors.forename: Tiziana
authors.initials: T
authors.lastname: D'Inzeo
authors.forename: Serena
authors.initials: S
authors.lastname: Campoli
authors.forename: Alberto
authors.initials: A
authors.lastname: Ruggeri
authors.forename: Mario
authors.initials: M
authors.lastname: Tumbarello
authors.forename: Giulia
authors.initials: G
authors.lastname: Canu
authors.forename: Enrico Maria
authors.initials: EM
authors.lastname: Trecarichi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Parisi
authors.forename: Mirella
authors.initials: M
authors.lastname: Tronci
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fadda
mesh_terms: D001769:Blood / Q000382:microbiology; D002175:Candida / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D058387:Candidemia / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
references.citation: Clin Microbiol Infect. 2010 May;16(5):493-500
references.pmid: 19624510
references.citation: PLoS One. 2009 Nov 25;4(11):e8041
references.pmid: 19946369
references.citation: Clin Microbiol Infect. 2011 Sep;17(9):1359-65
references.pmid: 20946411
references.citation: Clin Infect Dis. 2006 Jul 1;43(1):25-31
references.pmid: 16758414
references.citation: Clin Chem. 2011 Jul;57(7):1057-67
references.pmid: 21576270
references.citation: J Clin Microbiol. 2002 Oct;40(10):3551-7
references.pmid: 12354845
references.citation: J Clin Microbiol. 2010 Oct;48(10):3482-6
references.pmid: 20668126
references.citation: Pharmacotherapy. 2010 Apr;30(4):361-8
references.pmid: 20334456
references.citation: Anal Bioanal Chem. 2008 Oct;392(3):439-49
references.pmid: 18690424
references.citation: J Clin Microbiol. 2009 Jan;47(1):247-8
references.pmid: 18987173
references.citation: J Clin Microbiol. 2010 May;48(5):1542-8
references.pmid: 20237092
references.citation: J Clin Microbiol. 2011 Jul;49(7):2528-32
references.pmid: 21543564
references.citation: J Clin Microbiol. 2007 Apr;45(4):1343-6
references.pmid: 17287333
references.citation: Clin Microbiol Infect. 2010 Nov;16(11):1620-5
references.pmid: 20545958
references.citation: J Clin Microbiol. 2007 Jun;45(6):1843-50
references.pmid: 17460052
references.citation: Clin Infect Dis. 2009 Mar 1;48(5):503-35
references.pmid: 19191635
references.citation: J Clin Microbiol. 2009 Sep;47(9):2912-7
references.pmid: 19571014
references.citation: J Clin Microbiol. 2011 Jan;49(1):396-9
references.pmid: 21068282
references.citation: Clin Microbiol Rev. 2007 Jan;20(1):133-63
references.pmid: 17223626
references.citation: Clin Microbiol Infect. 2011 Apr;17(4):546-51
references.pmid: 20456452
references.citation: Diagn Microbiol Infect Dis. 2009 Aug;64(4):402-7
references.pmid: 19446982
references.citation: J Clin Microbiol. 2011 Apr;49(4):1614-6
references.pmid: 21270234
references.citation: Diagn Microbiol Infect Dis. 2007 Jul;58(3):325-31
references.pmid: 17350205
references.citation: PLoS One. 2010 Jan 25;5(1):e8862
references.pmid: 20111603