title: Evaluation of the relative cell surface charge by using microbial adhesion to hydrocarbon.
abstract: A simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference between these two affinities can provide the relative cell surface charge. The application of this method for Staphylococcus aureus and Escherichia coli show that the profile of surface charge evolution as a function ofpH was similar to these obtained by microelectrophoresis method.
authors.affiliation: Laboratoire de valorisation et sécurité des produits agroalimentaires, Faculté de Sciences et Techniques, Université Sultan Moulay Slimane, BP 523 Beni Mellal, Maroc.
authors.forename: Fatima
authors.initials: F
authors.lastname: Hamadi
authors.forename: Hassan
authors.initials: H
authors.lastname: Latrache
authors.forename: Hafida
authors.initials: H
authors.lastname: Zahir
authors.forename: Jamaa
authors.initials: J
authors.lastname: Bengourram
authors.forename: Nourreeddine
authors.initials: N
authors.lastname: Kouider
authors.forename: Abderrahmene
authors.initials: A
authors.lastname: Elghmari
authors.forename: Khalid
authors.initials: K
authors.lastname: Habbari
mesh_terms: D000473:Alkanes / Q000737:chemistry; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D002462:Cell Membrane / Q000737:chemistry; D004560:Electricity; D004926:Escherichia coli / Q000737:chemistry / Q000502:physiology; D006838:Hydrocarbons / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013211:Staphylococcus aureus / Q000737:chemistry / Q000502:physiology; D013499:Surface Properties
chemical_list: D000473:Alkanes; D006838:Hydrocarbons; C007932:n-hexadecane