title: [The study on protective effect of sphingosine-1-phosphate in cardiomyocytes].
abstract: OBJECTIVE
  To explore the mechanism of protective effect of Sphingosine-1-phosphate(S1P) in cultured neonatal rat cardiomyocytes dining simulated hypoxia/reoxygenation.
  
  
  METHODS
  On the basis of culturing neonatal rat cardiomyocytes, the model of hypoxia-reoxygennation was built by using method of Liquid Paraffin covering, the impact of S1P on apoptosis and p-Akt and mitochondrial membrane potential were studied by using method of Propidine Iodide staining and Western blot and Bhodanmine123 staining.
  
  
  RESULTS
  SiP could reduce apoptosis rate (P < 0.01) and stabilize the mitochondrial membrane potential (P < 0.05) and improved the level of p-Akt1 (P < 0.01) in hypoxia/reoxygenation cardiomyocytes significantly. But wonnannin could block these effects of S1P partially.
  
  
  CONCLUSIONS
  SiP can obviously restrain apoptosis in curtured rat neonatal cardiomyocytes during simulated hypoxia/reoxygenation. Stabilization of mitochondrial membrane potential by P13K-AM signaling pathway is likely to play a role in protective action of S1P.
authors.affiliation: Department of Physiology, Luohe Medical College, Luohe 462002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Duo-duo
authors.initials: DD
authors.lastname: Zhang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005260:Female; D008246:Lysophospholipids / Q000494:pharmacology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000166:cytology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D061251:Primary Cell Culture; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D008246:Lysophospholipids; D020011:Protective Agents; C060506:sphingosine 1-phosphate; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D013110:Sphingosine