title: Inhibitory constituents of Nardostachys chinensis on nitric oxide production in RAW 264.7 macrophages.
abstract: The activity-guided fractionation of the MeOH extract of the rhizomes and roots of Nardostachys chinensis led to the isolation of two new sesquiterpenoids, narchinol B (8) and narchinol C (9), along with 10 known compounds, ursolic acid (1), nardosinone (2), pinoresinol (3), desoxo-narchinol A (4), kanshone B (5), epoxyconiferyl alcohol (6), debilon (7), 4α,5-dimethyl-1,3-dioxo-1,2,3,4,4α,5,6,7-octahydronaphthalene (10), p-coumaric acid (11), and isoferulic acid (12). Their structures were determined using spectroscopic techniques, which included 1D- and 2D-NMR. Among the isolates, compounds 2, 4, 5, 8 and 9 showed inhibitory activity against LPS-induced NO production with IC(50) values of 4.6-21.6 μM.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Ji Sang
authors.initials: JS
authors.lastname: Hwang
authors.forename: Seon A
authors.initials: SA
authors.lastname: Lee
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Xiang Hua
authors.initials: XH
authors.lastname: Han
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Chong-Kil
authors.initials: CK
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D015195:Drug Design; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000737:chemistry; D008264:Macrophages / Q000166:cytology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008956:Models, Chemical; D032404:Nardostachys / Q000378:metabolism*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide; D000432:Methanol