title: Capacitive immunosensor for the detection of host cell proteins.
abstract: A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell are immobilized. Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a model system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing a negative influence from non-specific binding to the sensor surface.
authors.affiliation: Department of Biotechnology, Lund University, Box 124, S-22100 Lund, Sweden.
authors.forename: Kosin
authors.initials: K
authors.lastname: Teeparuksapun
authors.forename: Martin
authors.initials: M
authors.lastname: Hedström
authors.forename: Proespichaya
authors.initials: P
authors.lastname: Kanatharana
authors.forename: Panote
authors.initials: P
authors.lastname: Thavarungkul
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002417:Cattle; D025802:Electric Capacitance; D004566:Electrodes; D004867:Equipment Design; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008954:Models, Biological; D011994:Recombinant Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase