title: A transcriptomic analysis reveals the nature of salinity tolerance of a wheat introgression line.
abstract: The bread wheat cultivar Shanrong No.3 (SR3) is a salinity tolerant derivative of an asymmetric somatic hybrid between cultivar Jinan 177 (JN177) and tall wheatgrass (Thinopyrum ponticum). To reveal some of the mechanisms underlying its elevated abiotic stress tolerance, both SR3 and JN177 were exposed to iso-osmotic NaCl and PEG stress, and the resulting gene expression was analysed using a customized microarray. Some genes associated with stress response proved to be more highly expressed in SR3 than in JN177 in non-stressed conditions. Its unsaturated fatty acid and flavonoid synthesis ability was also enhanced, and its pentose phosphate metabolism was more active than in JN177. These alterations in part accounted for the observed shift in the homeostasis related to reactive oxygen species (ROS). The specific down-regulation of certain ion transporters after a 0.5 h exposure to 340 mM NaCl demonstrated that Na(+) uptake occurred rapidly, so that the early phase of salinity stress imposes more than simply an osmotic stress. We discussed the possible effect of the introgression of new genetic materials in wheat genome on stress tolerance.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, Shandong, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.forename: Mengcheng
authors.initials: M
authors.lastname: Wang
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D006823:Hybrid Vigor / Q000235:genetics; D006824:Hybridization, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D006109:Poaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D059467:Transcriptome; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome; D011092:Polyethylene Glycols; D012965:Sodium Chloride
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