title: A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature.
abstract: BACKGROUND
  Rice Xa3/Xa26 disease-resistance gene encodes a leucine-rich repeat (LRR) receptor kinase-type protein against Xanthomonas oryzae pv. oryzae (Xoo) and belongs to a multigene family. However, the functions of most genes in this family are unknown.
  
  
  RESULTS
  Here we report that two orthologs of this family, the NRKe from rice variety Nipponbare and 9RKe from variety 93-11 at the RKe locus, have similar functions although they encode different proteins. This pair of orthologs could not mediate resistance to Xoo, but they were transcriptionally induced by raised temperature. Transcriptional activation of NRKe or 9RKe resulted in the formation of temperature-sensitive lesion mimics, which were spots of dead cells associated with accumulation of superoxides, in different organs of the transgenic plants. These plants were more sensitive to high temperature shock than wild-type controls. Transgenic plants carrying a chimeric protein consisting of the LRR domain of NRKe and the kinase domain of Xa3/Xa26 developed the same lesion mimics as the NRKe-transgenic plants, whereas transgenic plants carrying another chimeric protein consisting of the LRR domain of Xa3/Xa26 and the kinase domain of NRKe were free of lesion mimic. All the transgenic plants carrying a chimeric protein were susceptible to Xoo.
  
  
  CONCLUSIONS
  These results suggest that the RKe locus is involved in rice response to raised temperature. The LRR domain of RKe protein appears to be important to sense increased temperature. The RKe-involved temperature-related pathway and Xa3/Xa26-mediated disease-resistance pathway may partially overlap.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Xianghua
authors.initials: X
authors.lastname: Li
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xiao
authors.forename: Shiping
authors.initials: S
authors.lastname: Wang
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D000090263:Leucine-Rich Repeat Proteins; D005810:Multigene Family; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases; D017422:Sequence Analysis, DNA; D013696:Temperature; D014974:Xanthomonas / Q000472:pathogenicity
chemical_list: D018744:DNA, Plant; D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D011494:Protein Kinases; D020794:Receptor Protein-Tyrosine Kinases; C486360:Xa26 protein, Oryza sativa
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