title: Survival role of superoxide dismutase 1 on human granulosa luteinized cells in vitro.
abstract: OBJECTIVE
  Deleterious effects of free radicals do not only result from the amount of free radicals produced but also are related to the efficiency and to the activities of enzymatic antioxidant systems. We investigated the effect of exogenous superoxide dismutase (SOD1) or Cu-chelating agent diethyldithiocarbamate (DDC) on the apoptosis (caspase-3 activity) of human granulosa luteinized cells (hGLC) in vitro.
  
  
  METHODS
  The effects of SOD1 and DDC were studied using in vitro culture system, caspase-3 and the total SOD activity in hGLCs were measured using AcDEVD-PNA substrate and Beaushap and Fridovich methods, respectively, after 48 h of the culture period.
  
  
  RESULTS
  The activity of SOD1 was the lowest in GLCs treated with 100 µM DDC as compared to control cells and to the cells supplemented with Cu, Zn-SOD or DDC (10 µM). The effect of DDC was associated with elevated caspase-3 activity as compared to control cells.
  
  
  CONCLUSIONS
  It was demonstrated for the first time that the supplementation of cultured hGLCs with Cu2+, Zn2+-SOD (200 U/ml) maintains the viability of hGLCs via caspase-3 suppression.
authors.affiliation: Bulgarian Academy of Sciences, Sophia, Bulgaria.
authors.forename: J
authors.initials: J
authors.lastname: Dineva
authors.forename: I
authors.initials: I
authors.lastname: Vangelov
authors.forename: R
authors.initials: R
authors.lastname: Abrashev
authors.forename: K
authors.initials: K
authors.lastname: Todorova
authors.forename: D
authors.initials: D
authors.lastname: Gulenova
authors.forename: D
authors.initials: D
authors.lastname: Nikolov
authors.forename: I
authors.initials: I
authors.lastname: Rangelov
authors.forename: G
authors.initials: G
authors.lastname: Stamenov
authors.forename: M
authors.initials: M
authors.lastname: Angelova
authors.forename: M
authors.initials: M
authors.lastname: Ivanova
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006107:Granulosa Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology*; D000072105:Superoxide Dismutase-1
chemical_list: D002614:Chelating Agents; D003470:Culture Media; C000606290:SOD1 protein, human; D004050:Ditiocarb; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; C505407:CASP3 protein, human; D053148:Caspase 3