title: Human embryonic stem cell-derived mesenchymal stromal cells.
abstract: Mesenchymal stromal cells (MSCs) originally isolated from marrow have multipotent differentiation potential and favorable immunomodulatory and anti-inflammatory properties that make them very attractive for regenerative cellular therapy. Cells with similar phenotypic characteristics have now been derived from almost all fetal, neonatal, and adult tissues; furthermore, more recently similar cells have also been generated from human embryonic stem cells (ESCs). Generation of MSCs from human ESCs provides an opportunity to study the developmental biology of human mesenchymal lineage generation in vitro. Generation of bone and cartilage from human ESC-derived MSCs and their functional characterization, both in vitro and in vivo, is also an active area of investigation as ESCs could provide an unlimited source of MSCs for potential repair of bone and cartilage defects. MSCs from adult sources are being investigated in numerous Phase I-III clinical trials for a wide variety of indications, mainly based on their immunomodulatory properties. Our group and others have shown MSCs derived from human ESCs possess immunomodulatory properties similar to marrow-derived MSCs. Immunomodulatory properties of ESC-derived MSCs could prove to be highly valuable for their potential clinical applications in the future as derivatives of human ESCs have already entered clinical arena in the context of Phase I clinical trials.
authors.affiliation: Department of Medicine, Division of Hematology/Oncology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin 53705-2275, USA. pxh@medicine.wisc.edu
authors.forename: Peiman
authors.initials: P
authors.lastname: Hematti
mesh_terms: D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D017321:Clinical Trials, Phase I as Topic; D017322:Clinical Trials, Phase II as Topic; D018920:Coculture Techniques / Q000379:methods; D058732:Embryoid Bodies / Q000166:cytology / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D044968:Regenerative Medicine / Q000379:methods / Q000639:trends; D046509:Tissue Culture Techniques; D057170:Translational Research, Biomedical / Q000379:methods / Q000639:trends
references.citation: Lancet. 2004 May 1;363(9419):1439-41
references.pmid: 15121408
references.citation: Cytotherapy. 2007;9(3):283-91
references.pmid: 17464760
references.citation: Am Surg. 1999 Jan;65(1):22-6
references.pmid: 9915526
references.citation: Stem Cells. 2009 Mar;27(3):670-681
references.pmid: 19267325
references.citation: Transplantation. 2005 Jun 15;79(11):1607-14
references.pmid: 15940052
references.citation: Transplantation. 1968 Mar;6(2):230-47
references.pmid: 5654088
references.citation: Stem Cells. 2006 Apr;24(4):835-43
references.pmid: 16253980
references.citation: Transplantation. 2009 May 15;87(9 Suppl):S36-42
references.pmid: 19424002
references.citation: Methods Mol Biol. 2011;690:163-74
references.pmid: 21042992
references.citation: Bone Marrow Transplant. 2002 Aug;30(4):215-22
references.pmid: 12203137
references.citation: Diabetes. 2008 Jul;57(7):1759-67
references.pmid: 18586907
references.citation: J Cell Physiol. 2007 Apr;211(1):27-35
references.pmid: 17226788
references.citation: Blood. 2007 Nov 15;110(10):3499-506
references.pmid: 17664353
references.citation: Exp Hematol. 2007 Jan;35(1):146-54
references.pmid: 17198883
references.citation: Ann Neurol. 2005 Jun;57(6):874-82
references.pmid: 15929052
references.citation: Cytotherapy. 2009;11(5):503-15
references.pmid: 19728189
references.citation: Blood. 2001 Oct 15;98(8):2396-402
references.pmid: 11588036
references.citation: Expert Opin Biol Ther. 2006 Sep;6(9):847-66
references.pmid: 16918253
references.citation: Science. 1998 Nov 6;282(5391):1145-7
references.pmid: 9804556
references.citation: Stem Cells. 2009 Feb;27(2):451-6
references.pmid: 18988708
references.citation: Exp Hematol. 2009 Dec;37(12):1445-53
references.pmid: 19772890
references.citation: Tissue Eng. 2001 Apr;7(2):211-28
references.pmid: 11304456
references.citation: Nature. 2008 Apr 10;452(7188):670
references.pmid: 18401364
references.citation: Stem Cells. 2010 Aug;28(8):1446-55
references.pmid: 20597105
references.citation: Curr Opin Pharmacol. 2009 Jun;9(3):268-73
references.pmid: 19349209
references.citation: Biol Blood Marrow Transplant. 2005 May;11(5):389-98
references.pmid: 15846293
references.citation: Stem Cells. 2004;22(7):1338-45
references.pmid: 15579651
references.citation: Cytotherapy. 2009;11(1):18-25
references.pmid: 19012065
references.citation: Stem Cells. 2005 Oct;23(9):1221-7
references.pmid: 15955827
references.citation: Transplant Rev (Orlando). 2008 Oct;22(4):262-73
references.pmid: 18656340
references.citation: Blood. 2002 May 15;99(10):3838-43
references.pmid: 11986244
references.citation: Trends Biotechnol. 2000 Feb;18(2):53-7
references.pmid: 10652509
references.citation: Stem Cells. 2006 Jun;24(6):1458-66
references.pmid: 16456136
references.citation: Stem Cells. 2004;22(6):972-80
references.pmid: 15536188
references.citation: Handb Exp Pharmacol. 2007;(180):195-218
references.pmid: 17554510
references.citation: Biol Blood Marrow Transplant. 2005 May;11(5):321-34
references.pmid: 15846285
references.citation: Cytotherapy. 2005;7(1):36-45
references.pmid: 16040382
references.citation: Curr Opin Investig Drugs. 2006 May;7(5):473-81
references.pmid: 16729725
references.citation: Am J Cardiol. 2004 Jul 1;94(1):92-5
references.pmid: 15219514
references.citation: Tissue Eng. 2004 Sep-Oct;10(9-10):1518-25
references.pmid: 15588411
references.citation: J Cell Physiol. 2007 Nov;213(2):341-7
references.pmid: 17620285
references.citation: Stem Cells. 2011 Feb;29(2):251-62
references.pmid: 21732483
references.citation: Transplantation. 2003 Oct 27;76(8):1208-13
references.pmid: 14578755
references.citation: Tissue Eng. 2006 Sep;12(9):2695-706
references.pmid: 16995803
references.citation: Stem Cells. 2003;21(2):131-42
references.pmid: 12634409
references.citation: Blood. 2007 Apr 15;109(8):3147-51
references.pmid: 17170129
references.citation: Stem Cells. 2007 Apr;25(4):950-60
references.pmid: 17218402
references.citation: Plast Reconstr Surg. 2008 Mar;121(3):860-877
references.pmid: 18317135
references.citation: Lancet. 2008 May 10;371(9624):1579-86
references.pmid: 18468541
references.citation: Cytotherapy. 2006;8(4):315-7
references.pmid: 16923606
references.citation: Leukemia. 2008 May;22(5):1062-4
references.pmid: 17972956
references.citation: Science. 1999 Apr 2;284(5411):143-7
references.pmid: 10102814
references.citation: Handb Exp Pharmacol. 2007;(180):45-66
references.pmid: 17554504
references.citation: Stem Cells. 2004;22(4):625-34
references.pmid: 15277708
references.citation: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
references.pmid: 11087820
references.citation: Cell Stem Cell. 2009 Jul 2;5(1):54-63
references.pmid: 19570514
references.citation: Curr Opin Hematol. 2006 Nov;13(6):419-25
references.pmid: 17053453
references.citation: Stem Cells. 2006 Aug;24(8):1914-22
references.pmid: 16644919
references.citation: Bone Marrow Transplant. 1995 Oct;16(4):557-64
references.pmid: 8528172
references.citation: PLoS Med. 2005 Jun;2(6):e161
references.pmid: 15971941
references.citation: Methods Cell Biol. 2008;86:15-28
references.pmid: 18442642
references.citation: Nat Med. 2009 Jan;15(1):42-9
references.pmid: 19098906
references.citation: Stem Cells. 2005 Mar;23(3):306-14
references.pmid: 15749925
references.citation: Methods Mol Biol. 2011;690:1-30
references.pmid: 21042982
references.citation: Stem Cells Dev. 2006 Dec;15(6):865-79
references.pmid: 17253949
references.citation: Exp Hematol. 2003 Oct;31(10):890-6
references.pmid: 14550804
references.citation: Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16875-80
references.pmid: 20837529
references.citation: Nat Med. 1999 Mar;5(3):309-13
references.pmid: 10086387
references.citation: Blood. 2003 Apr 15;101(8):2999-3001
references.pmid: 12480709
references.citation: Exp Hematol. 2008 Mar;36(3):350-9
references.pmid: 18179856
references.citation: Tissue Cell. 2005 Aug;37(4):325-34
references.pmid: 15979113
references.citation: Stem Cells. 2007 Feb;25(2):425-36
references.pmid: 17053208
references.citation: Ann Rheum Dis. 2010 Aug;69(8):1423-9
references.pmid: 20650877
references.citation: Cloning Stem Cells. 2003;5(2):149-55
references.pmid: 12930627
references.citation: Invest Ophthalmol Vis Sci. 2004 Dec;45(12):4251-5
references.pmid: 15557428
references.citation: Cell Stem Cell. 2010 Oct 8;7(4):431-42
references.pmid: 20887949
references.citation: Scand J Immunol. 2003 Jan;57(1):11-20
references.pmid: 12542793