title: [Study on alternative methods for the acute oral toxicity in detection of chemicals].
abstract: OBJECTIVE
  To evaluate three alternative methods for LD50 test-Fixed Dose Procedure (FDP), the Acute Toxic Class Method (ATC) and Up and Down Procedure (UDP).
  
  
  METHODS
  Female SD rats (8-12 weeks of age, 160-200 g) were used. Three alternative methods from OECD were applied to assess 22 chemicals (10 cosmetic raw materials and 12 raw materials of personal and home care products). The toxicity ranking for tested chemicals was established according to Globally Harmonized System (GSH). The results LD50 test were compared for the consistency and correlation between alternative methods and traditional test.
  
  
  RESULTS
  For cosmetic raw materials, the concordance rate of the three alternative methods was 80% (8/10); for raw material of personal and home care products, the concordance rates of FDP, ATC and UDP was 91.7% (11/12), 75.0% (9/12) and 83.0% (10/12), respectively. The number of animals required in three alternative methods was significantly lower than that in traditional test (P < 0.05), but the time required in three alternative methods was significantly higher than that in traditional test (P < 0.05).
  
  
  CONCLUSIONS
  High consistency and correlation were found between each alternative method and LD50 test. FDP may be more potential when applied to assess acute oral toxicity of cosmetic raw materials.
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China.
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Tan
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Xing-Fen
authors.initials: XF
authors.lastname: Yang
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Zhou
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Chen
authors.forename: Rui-Yi
authors.initials: RY
authors.lastname: Chen
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003358:Cosmetics / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D015386:Hazardous Substances / Q000633:toxicity*; D007928:Lethal Dose 50; D051381:Rats; D017207:Rats, Sprague-Dawley; D023382:Toxicity Tests, Acute / Q000379:methods*
chemical_list: D003358:Cosmetics; D015386:Hazardous Substances