title: Multiplexed immunoassay of thyroglobulin autoantibodies in patients with differentiated thyroid carcinoma.
abstract: BACKGROUND
  Thyroglobulin (Tg) antibodies (TgAb) screening is recommended in patients with differentiated thyroid carcinoma (DTC) to validate Tg measurements. We compared a new multiplexed and 2 conventional TgAb immunoassays in patients with DTC and healthy controls.
  
  
  METHODS
  TgAb were measured by 2 conventional automated immunoassays and a multiplexed immunoassay in sera from 163 patients with DTC and 64 controls.
  
  
  RESULTS
  No significant differences were found between the different assays when the suggested manufacturer cutoffs were used. The positive rate of multiplexed assay, but not of conventional assays, significantly increased when limits of detection were used as cutoff.
  
  
  CONCLUSIONS
  Further technical evaluations are necessary to explain the very high rate of detectable TgAb values in both patients with DTC and controls by the multiplexed assay. For the moment, we suggest caution in using such methods in the current clinical practice.
authors.affiliation: Department of Nuclear Medicine and Thyroid Centre, Oncology Institute of Southern Switzerland, CH-6500 Bellinzona, Switzerland. luca.giovanella@eoc.ch
authors.forename: Luca
authors.initials: L
authors.lastname: Giovanella
authors.forename: Emanuela
authors.initials: E
authors.lastname: Toffalori
authors.forename: Renato
authors.initials: R
authors.lastname: Tozzoli
authors.forename: Marco
authors.initials: M
authors.lastname: Caputo
authors.forename: Luca
authors.initials: L
authors.lastname: Ceriani
authors.forename: Frederik A
authors.initials: FA
authors.lastname: Verburg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D016022:Case-Control Studies; D015331:Cohort Studies; D016001:Confidence Intervals; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013954:Thyroglobulin / Q000097:blood / Q000276:immunology*; D013964:Thyroid Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D013954:Thyroglobulin