title: [The effect of genetic polymorphism of the exon 2 of the beta-lactoglobulin gene on the milk composition in Chinese Holstein].
abstract: OBJECTIVE
  In order to study the effect of the polymorphism at the exon2 region of the (3-LG allele gene on milk composition and yield.
  
  
  METHODS
  The single-strand conformation polymorphism method (PCR-SSCP) was used to analyze for polymorphism the exon2 region of the 3-LG gene (NCBI accession number: DQ489319) in Chinese Holstein.
  
  
  RESULTS
  Eight SSCP patterns were detected in the fragments: ab, abc, abd, abe, abcd, abce, abde and abcde, and the patterns frequencies as follows: 0.14, 0.10, 0.27, 0.23, 0.05, 0.04, 0.11 and 0.06 (P < 0.05); Six single nucleotide polymorphism (SNP) were detected in this study: sitel C>T, site2 T>C, site3 C>T, site4 C>C, site5 C> A, site6 A>T or C, and the polymorphism infonnation content (PIC) of these SNPs were in median or high polymorphism (PIC > 0.25).
  
  
  CONCLUSIONS
  These SNPs at the exon2 region of the beta-LG gene were remarkably and affected milk performance traits (milk yield, protein and fat contents) in Chinese Holstein.
authors.affiliation: College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Zhou
authors.forename: Nan-qiao
authors.initials: NQ
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Cheng
authors.forename: Xi-wen
authors.initials: XW
authors.lastname: Huang
authors.forename: Ya-fei
authors.initials: YF
authors.lastname: Cai
authors.forename: Gen-lin
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000145:classification / Q000235:genetics*; D002681:China; D005091:Exons; D005260:Female; D007782:Lactoglobulins / Q000235:genetics*; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D007782:Lactoglobulins