title: Acute oxidative stress affects IL-8 and TNF-α expression in IPEC-J2 porcine epithelial cells.
abstract: Reactive oxygen species are implicated in cell and tissue damage in a number of diseases including acute and chronic inflammation of the gut. Effects of H(2)O(2) exposure on non-carcinogenic porcine epithelial cell line, IPEC-J2 cells cultured on collagen-coated membrane inserts were monitored based on transepithelial electrical resistance (TER) change, extent of necrotic cell damage, gene expression of inflammatory cytokines IL-8 and TNF-α. Furthermore, the junction proteins claudin-1 and E-cadherin were also investigated by immunohistochemistry. Peroxide (1mM) increased IL-8 and TNF-α gene expression levels significantly allowing 1 h recovery time without affecting the cellular distribution of junction proteins, TER and cell survival rate. In conclusion, the IPEC-J2 cell line on membrane insert was introduced as a fast and reliable investigation tool for oxidative stimuli-triggered intestinal inflammation and in the future as a screening method for antioxidant and probiotic candidates.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Sciences, Szent István University, Budapest, István u. 2 1078, Hungary. Gere.Erzsebet@aotk.szie.hu
authors.forename: Erzsebet
authors.initials: E
authors.lastname: Paszti-Gere
authors.forename: Edina
authors.initials: E
authors.lastname: Csibrik-Nemeth
authors.forename: Krisztina
authors.initials: K
authors.lastname: Szeker
authors.forename: Rita
authors.initials: R
authors.lastname: Csizinszky
authors.forename: Csaba
authors.initials: C
authors.lastname: Jakab
authors.forename: Peter
authors.initials: P
authors.lastname: Galfi
mesh_terms: D000818:Animals; D015820:Cadherins / Q000096:biosynthesis / Q000378:metabolism; D002460:Cell Line; D062445:Claudin-1; D017097:Electric Impedance; D004847:Epithelial Cells / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000494:pharmacology; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism; D009336:Necrosis; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D015820:Cadherins; D062445:Claudin-1; D015850:Interleukin-6; D016209:Interleukin-8; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha; D006861:Hydrogen Peroxide
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