title: Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins.
abstract: A macroporous boronate affinity monolithic column was prepared and applied to specifically capture glycoproteins using metal-organic gels (MOGs) as a porogenic template. This newly explored application of MOGs has proven to be a more convenient method for the formation of macropores in contrast to traditional porogenic methods. The poly (3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) monolithic columns were synthesized in stainless columns by in situ polymerization. To fabricate the macroporous formation with a uniformed open-channel network, the preparation conditions, such as reaction temperature, the concentration of the MOGs and the ratio of monomers were systematically investigated. The prepared macroporous monoliths were characterized by scanning electron microscope (SEM) and mercury intrusion porosimetry. Furthermore, horseradish peroxidase (HRP) and transferrin (TF) were chosen as test glycoproteins, and the chromatographic analysis demonstrated that the macroporous boronate affinity monoliths exhibited a higher selectivity and better dynamic binding capacity toward glycoproteins compared with non-glycoproteins. The resulted affinity monolithic column was successfully employed to specifically capture TF from a bovine serum sample.
authors.affiliation: College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiwen
authors.initials: X
authors.lastname: He
authors.forename: Langxing
authors.initials: L
authors.lastname: Chen
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002396:Catechols / Q000032:analysis; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000302:isolation & purification; D006873:Hydroquinones / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011312:Pressure; D013696:Temperature; D014168:Transferrin / Q000302:isolation & purification
chemical_list: D000180:Acrylic Resins; D001897:Boronic Acids; D002396:Catechols; D006023:Glycoproteins; D006873:Hydroquinones; D014168:Transferrin; C548049:poly(3-acrylamidophenylboronic acid-co-ethylene dimethacrylate); D006735:Horseradish Peroxidase; C034221:catechol