title: Connecting μ-fluidics to electron microscopy.
abstract: A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.
authors.affiliation: Center for Cellular Imaging and Nano Analytics (C-CINA), Biozentrum, Universität Basel, Basel, Switzerland.
authors.forename: Simon
authors.initials: S
authors.lastname: Kemmerling
authors.forename: Jörg
authors.initials: J
authors.lastname: Ziegler
authors.forename: Gabriel
authors.initials: G
authors.lastname: Schweighauser
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Arnold
authors.forename: Dominic
authors.initials: D
authors.lastname: Giss
authors.forename: Shirley A
authors.initials: SA
authors.lastname: Müller
authors.forename: Philippe
authors.initials: P
authors.lastname: Ringler
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Goldie
authors.forename: Nils
authors.initials: N
authors.lastname: Goedecke
authors.forename: Andreas
authors.initials: A
authors.lastname: Hierlemann
authors.forename: Henning
authors.initials: H
authors.lastname: Stahlberg
authors.forename: Andreas
authors.initials: A
authors.lastname: Engel
authors.forename: Thomas
authors.initials: T
authors.lastname: Braun
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D044085:Microfluidics / Q000295:instrumentation*; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods*; D013194:Staining and Labeling; D049490:Systems Biology / Q000379:methods