title: Identification of non-diphtheriae corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of ≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Adnan A
authors.initials: AA
authors.lastname: Alatoom
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Cazanave
authors.forename: Scott A
authors.initials: SA
authors.lastname: Cunningham
authors.forename: Sherry M
authors.initials: SM
authors.lastname: Ihde
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D003352:Corynebacterium / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000175:diagnosis* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
references.citation: J Clin Microbiol. 2004 Sep;42(9):3925-31
references.pmid: 15364970
references.citation: J Clin Microbiol. 2011 Aug;49(8):2868-73
references.pmid: 21613431
references.citation: J Clin Microbiol. 2004 Sep;42(9):4189-98
references.pmid: 15365010
references.citation: J Clin Microbiol. 1998 Nov;36(11):3399-407
references.pmid: 9774606
references.citation: Cardiovasc Pathol. 2003 Mar-Apr;12(2):98-101
references.pmid: 12684166
references.citation: J Clin Microbiol. 2011 Feb;49(2):693-6
references.pmid: 21106794
references.citation: J Infect. 2007 Feb;54(2):e79-81
references.pmid: 16757031
references.citation: J Clin Microbiol. 2005 Apr;43(4):1934-6
references.pmid: 15815024
references.citation: Eur J Clin Microbiol Infect Dis. 2006 Jun;25(6):349-53
references.pmid: 16767481
references.citation: J Med Microbiol. 2011 Jul;60(Pt 7):1038-1042
references.pmid: 21393451
references.citation: Scand J Infect Dis. 2005;37(2):151-3
references.pmid: 15773035
references.citation: BMC Infect Dis. 2007 Feb 06;7:4
references.pmid: 17284316
references.citation: BMC Genomics. 2010 Feb 05;11:91
references.pmid: 20137072
references.citation: Euro Surveill. 2010 Oct 28;15(43):
references.pmid: 21087580
references.citation: Diagn Microbiol Infect Dis. 2009 Jun;64(2):213-5
references.pmid: 19304438
references.citation: Int J Infect Dis. 2008 Nov;12(6):672-4
references.pmid: 18346922
references.citation: J Clin Microbiol. 2011 Mar;49(3):887-92
references.pmid: 21209160
references.citation: J Clin Microbiol. 2011 Apr;49(4):1614-6
references.pmid: 21270234
references.citation: Diagn Microbiol Infect Dis. 1993 Jul;17(1):23-8
references.pmid: 8359002
references.citation: J Med Microbiol. 2008 Oct;57(Pt 10):1299-1302
references.pmid: 18809563
references.citation: Cases J. 2008 Dec 12;1(1):388
references.pmid: 19077258