title: Newly identified genetic variations in common Escherichia coli MG1655 stock cultures.
abstract: We have recently identified seven mutations in commonly used stocks of the sequenced Escherichia coli strain MG1655 which do not appear in the reference sequence. The mutations are likely to cause loss of function of the glpR and crl genes, which may have serious implications for physiological experiments using the affected strains.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
authors.forename: Lydia
authors.initials: L
authors.lastname: Freddolino
authors.forename: Sasan
authors.initials: S
authors.lastname: Amini
authors.forename: Saeed
authors.initials: S
authors.lastname: Tavazoie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D048168:Escherichia coli K12 / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D014644:Genetic Variation; D005994:Glycerophosphates / Q000378:metabolism; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012808:Sigma Factor / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C101651:GlpR protein, E coli; D005994:Glycerophosphates; D012097:Repressor Proteins; D012808:Sigma Factor; C077481:Crl protein, Bacteria; C029620:alpha-glycerophosphoric acid; D012321:DNA-Directed RNA Polymerases
references.citation: PLoS Genet. 2009 Nov;5(11):e1000713
references.pmid: 19893610
references.citation: Science. 1997 Sep 5;277(5331):1453-62
references.pmid: 9278503
references.citation: J Bacteriol. 2004 Oct;186(19):6437-42
references.pmid: 15375124
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D464-70
references.pmid: 18974181
references.citation: Mol Syst Biol. 2006;2:2006.0007
references.pmid: 16738553
references.citation: Infect Immun. 2011 Jun;79(6):2430-9
references.pmid: 21422176
references.citation: Genome Res. 2011 Aug;21(8):1388-93
references.pmid: 21555365
references.citation: Mol Microbiol. 1992 Jan;6(1):47-57
references.pmid: 1738314
references.citation: J Bacteriol. 2001 Feb;183(4):1459-61
references.pmid: 11157961
references.citation: Appl Environ Microbiol. 2010 Jul;76(13):4158-68
references.pmid: 20435762
references.citation: EMBO J. 2007 Mar 21;26(6):1569-78
references.pmid: 17332743
references.citation: Bioinformatics. 2009 Jul 15;25(14):1754-60
references.pmid: 19451168
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19462-7
references.pmid: 19052235
references.citation: J Bacteriol. 2004 Nov;186(22):7529-37
references.pmid: 15516564
references.citation: J Bacteriol. 2003 Sep;185(18):5611-26
references.pmid: 12949114
references.citation: J Bacteriol. 2006 Nov;188(22):7966-70
references.pmid: 16980472
references.citation: Mol Cell Proteomics. 2011 Oct;10(10):M111.007930
references.pmid: 21719796
references.citation: J Mol Biol. 1987 May 5;195(1):125-42
references.pmid: 2821267
references.citation: J Bacteriol. 1996 Dec;178(23):6790-5
references.pmid: 8955298
references.citation: Bioinformatics. 2009 Aug 15;25(16):2078-9
references.pmid: 19505943
references.citation: J Bacteriol. 1986 Nov;168(2):775-9
references.pmid: 3536860
references.citation: Mol Gen Genet. 1977 Mar 28;152(1):83-91
references.pmid: 325390
references.citation: FEBS Lett. 1994 Apr 18;343(1):65-9
references.pmid: 8163020
references.citation: Nature. 2009 Oct 29;461(7268):1243-7
references.pmid: 19838166