title: Evaluation of the performance of 5 commercialized enzyme immunoassays for the detection of Taenia solium antibodies and for the diagnosis of neurocysticercosis.
abstract: This study aimed to evaluate 5 enzyme immunoassays for detecting human antibodies against Taenia solium in human serum and for the diagnosis of neurocysticercosis (NCC): DRG™, RIDASCREEN™, NOVATECH™, CYPRESS™, and IVD™. A collection of 114 reference serum samples were used. All sera were tested both by ELISA and by an immunoblot method (enzyme-linked immunoelectrotransfer blot [EITB]). When compared with EITB, the Ridascreen™ test had the best positive concordance rate (85.1-91.2%) and the NovaLisa test™ showed the optimal negative concordance rate (93.7-95.6%). All tests had a sensitivity under 72% and a specificity above 60%. The best sensitivity was obtained using Ridascreen™ test (71.4%). An optimal specificity was achieved by the NovaLisa test™. T. solium-positive sera all cross-reacted with E. granulosus positive samples. In the commercial assays evaluated here, the most appropriate ELISA test for screening may be the Ridascreen™ assay. Antibody detection seems to be not appropriate for NCC diagnosis because of its overall lack of sensitivity.
authors.affiliation: Institut Pasteur de Madagascar, BP 1274 Antananarivo 101, Madagascar. jfcarod@yahoo.es
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Carod
authors.forename: Mickaël
authors.initials: M
authors.lastname: Randrianarison
authors.forename: Julien
authors.initials: J
authors.lastname: Razafimahefa
authors.forename: Rondro Mamitiana
authors.initials: RM
authors.lastname: Ramahefarisoa
authors.forename: Mahenintsoa
authors.initials: M
authors.lastname: Rakotondrazaka
authors.forename: Monique
authors.initials: M
authors.lastname: Debruyne
authors.forename: Mélanie
authors.initials: M
authors.lastname: Dautigny
authors.forename: Pierrette
authors.initials: P
authors.lastname: Cazal
authors.forename: Marcellin Lalaoarisoa
authors.initials: ML
authors.lastname: Andriantseheno
authors.forename: Emile Ramarokoto
authors.initials: ER
authors.lastname: Charles
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D020019:Neurocysticercosis / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D041201:Taenia solium / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000909:Antibodies, Helminth