title: Pathogen detection using engineered bacteriophages.
abstract: Bacteriophages, or phages, are bacterial viruses that can infect a broad or narrow range of host organisms. Knowing the host range of a phage allows it to be exploited in targeting various pathogens. Applying phages for the identification of microorganisms related to food and waterborne pathogens and pathogens of clinical significance to humans and animals has a long history, and there has to some extent been a recent revival in these applications as phages have become more extensively integrated into novel detection, identification, and monitoring technologies. Biotechnological and genetic engineering strategies applied to phages are responsible for some of these new methods, but even natural unmodified phages are widely applicable when paired with appropriate innovative detector platforms. This review highlights the use of phages as pathogen detector interfaces to provide the reader with an up-to-date inventory of phage-based biodetection strategies.
authors.affiliation: The University of Tennessee Center for Environmental Biotechnology, 676 Dabney Hall, Knoxville, TN 37996, USA.
authors.forename: Abby E
authors.initials: AE
authors.lastname: Smartt
authors.forename: Tingting
authors.initials: T
authors.lastname: Xu
authors.forename: Patricia
authors.initials: P
authors.lastname: Jegier
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Carswell
authors.forename: Samuel A
authors.initials: SA
authors.lastname: Blount
authors.forename: Gary S
authors.initials: GS
authors.lastname: Sayler
authors.forename: Steven
authors.initials: S
authors.lastname: Ripp
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*; D001424:Bacterial Infections / Q000382:microbiology*; D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005516:Food Microbiology; D005818:Genetic Engineering; D006801:Humans; D014871:Water Microbiology