title: Cyclooxygenase-2 -765G>C polymorphism is associated with C-reactive protein levels in resistant smokers but not in chronic obstructive pulmonary disease patients.
abstract: We sought to investigate whether the serum concentrations of several inflammatory biomarkers are related to the cyclooxygenase-2 (COX2) -765G>C polymorphism in chronic obstructive pulmonary disease (COPD) and a control group of non-COPD smokers. Serum inflammatory markers (CRP, SAA, CXCL8, and sICAM-1) were measured by ELISA in 144 patients with COPD and in 55 control subjects. Genomic DNA was extracted from peripheral blood leukocytes, and the COX2 -765G>C (rs20417) polymorphism was genotyped. After adjustment for age and active smoking, CRP and SAA concentrations were associated with the COX2 polymorphism in controls (p=0.041 and 0.014, respectively) but not in COPD patients. The CXCL8 and sICAM-1 concentrations were not associated with the COX2 polymorphism for either cases or controls. The results of the present study indicate that there is a relationship between the COX2 -765G>C polymorphism and the concentrations of CRP and SAA in non-COPD smokers and that this relationship does not exist in COPD patients.
authors.affiliation: Unidad Médico-Quirúrgica de Enfermedades Respiratorias, Hospital Universitario Virgen del Rocío, Sevilla, Spain. lcampos@separ.es
authors.forename: José Luis
authors.initials: JL
authors.lastname: López-Campos
authors.forename: Ana
authors.initials: A
authors.lastname: Montes-Worboys
authors.forename: Carmen
authors.initials: C
authors.lastname: Calero
authors.forename: Eduardo
authors.initials: E
authors.lastname: Márquez-Martin
authors.forename: Pilar
authors.initials: P
authors.lastname: Cejudo
authors.forename: Francisco
authors.initials: F
authors.lastname: Ortega
authors.forename: Elena
authors.initials: E
authors.lastname: Arellano
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000276:immunology; D012907:Smoking / Q000097:blood / Q000276:immunology*
chemical_list: D015415:Biomarkers; D000685:Serum Amyloid A Protein; D002097:C-Reactive Protein; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human