title: Diluted povidone-iodine inhibits tumor growth through apoptosis-induction             and suppression of SOD activity.
abstract: Povidone-iodine (PVP-I) is widely used in clinical practice as an antiseptic             and flushing agent after surgery to remove a tumor. Our present study was designed             to determine whether diluted PVP-I is cytotoxic to colon cancer cells and ascetic             tumor cells in vitro and to examine its antitumor effects in vivo. In vitro, CT26             and H22 cells treated with different concentrations of diluted PVP-I (0-1.56 µg/ml)             were analyzed using the mononuclear cell direct cytotoxicity assay (MTT) and a             flow cytometry assay. In vivo, Balb/c mice injected in the abdominal cavity with             CT26 cells or H22 cells were treated intraperitoneally with different concentrations             of PVP-I (0-312.5 µg/mouse), cisplatin (40 mg/kg) or 5'-FU (30 mg/kg) or left             untreated. In vitro, the studies demonstrated the antiproliferative and significant             apoptosis-inducing effects of PVP-I in a dose- and time-dependent manner. In vivo,             PVP-I significantly repressed the growth of H22 and CT26 cells in Balb/c mice             compared to controls. To explore the mechanism of the antitumor effect of PVP-I,             the superoxide dismutase (SOD) activity of ascites extracted from a mouse model             and the supernatant of CT26 cells was detected by an SOD kit. The activity of             SOD was significantly inhibited in the experimental groups. Taken together, our             data suggest that PVP-I exhibits a strong inhibitory effect on tumor growth in             colon cancer (CT26) and hepatoma (H22) resulting from apoptosis, both in vitro             and in vivo, suggesting a new potential therapeutic approach after tumor excision             surgery to colon cancer and hepatoma.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Chengdu 610041, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
authors.forename: Ju-Mei
authors.initials: JM
authors.lastname: Zhao
authors.forename: Zi-Chao
authors.initials: ZC
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Luo
authors.forename: Da-Bing
authors.initials: DB
authors.lastname: Yang
authors.forename: Jiao
authors.initials: J
authors.lastname: Tan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Yong-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology*; D011206:Povidone-Iodine / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors*
chemical_list: D000970:Antineoplastic Agents; D011206:Povidone-Iodine; D013482:Superoxide Dismutase