title: Toward a low-cost compact array microscopy platform for detection of tuberculosis.
abstract: This paper describes the development of a microscope array capable of imaging separate fields of view without the need for opto-mechanical scanning components. This microscope array can be integrated with array illuminating optics, a full frame digital single lens reflex (DSLR) camera, and automated algorithms for the detection of Mycobacterium tuberculosis (MTB). The entire array will fit within the area of a typical sputum smear. A custom miniature objective has been designed for this microscope array that has a numerical aperture of 0.5, optical resolution of 0.63 μm, and a field of view that is 0.54 mm in diameter. A single prototype miniature objective of this design has been built, and images are presented demonstrating its imaging performance. Images are sufficiently high quality for diagnostic use. When fully integrated, this device has the potential to significantly improve performance compared to conventional microscopy systems and to enable more effective diagnosis of tuberculosis at the point of care.
authors.affiliation: Department of Bioengineering, Rice University, Houston, TX 77030-1402, USA. brian.mccall@rice.edu
authors.forename: Brian
authors.initials: B
authors.lastname: McCall
authors.forename: Mark
authors.initials: M
authors.lastname: Pierce
authors.forename: Edward A
authors.initials: EA
authors.lastname: Graviss
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.forename: Tomasz
authors.initials: T
authors.lastname: Tkaczyk
mesh_terms: D003365:Costs and Cost Analysis; D004867:Equipment Design; D006801:Humans; D007909:Lenses; D008853:Microscopy / Q000191:economics / Q000295:instrumentation*; D008904:Miniaturization / Q000295:instrumentation; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis*