title: A respiratory syncytial virus isolate enables the testing of virucidal products.
abstract: The respiratory syncytial virus (RSV) is known as a major cause of respiratory infections and nosocomial diseases. Testing this virus is rather difficult due to the problems encountered in producing it at a high titer without using any purification method. A RSV isolate which replicates to high level on a Hep-2 cell line with an infectious titer of at least 10(7)TCID(50)mL(-1) in culture supernatant fluids has been identified. Thanks to this isolate, the virucidal effects of two products, a hand rub solution and a surface disinfectant, were conveniently tested according to the EN 14476:2007-02 procedure.
authors.affiliation: Laboratory of Virology/EA3610, University Lille 2, Faculty of Medecine, CHRU Lille, 59037 Lille, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Thevenin
authors.forename: P E
authors.initials: PE
authors.lastname: Lobert
authors.forename: A
authors.initials: A
authors.lastname: Dewilde
authors.forename: D
authors.initials: D
authors.lastname: Hober
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004202:Disinfectants / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012136:Respiratory Syncytial Viruses / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology; D014776:Virus Cultivation
chemical_list: D000998:Antiviral Agents; D004202:Disinfectants