title: Ripe fruit of Rubus coreanus inhibits mast cell-mediated allergic inflammation.
abstract: In this study, we investigated the effect of a water extract of the ripe fruits of Rubus coreanus Miq. (Rosaceae) (RFRC) on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, rhinitis, asthma and atopic dermatitis. RFRC dose-dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. RFRC reduced the immunoglobulin E (IgE)-mediated local allergic reaction, passive cutaneous anaphylaxis. RFRC attenuated histamine release from rat peritoneal mast cells and human mast cells by the reduction of intracellular calcium. RFRC decreased the phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187 (PMACI)-stimulated expression and secretion of pro-inflammatory cytokines in human mast cells. The inhibitory effect of RFRC on cytokine production was nuclear factor (NF)-κB- and mitogen-activated protein kinase (MAPK)-dependent. In addition, RFRC suppressed the activation of caspase-1. Our findings provide evidence that RFRC inhibits mast cell-derived allergic inflammatory reactions, and for the involvement of calcium, NF-κB, MAPKs and caspase-1 in these effects. Furthermore, in vivo and in vitro anti-allergic inflammatory effects of RFRC provide affirmative proof of a possible therapeutic application of this agent in allergic inflammatory diseases.
authors.affiliation: Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.forename: Phil Hyung
authors.initials: PH
authors.lastname: Choi
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Yoo
authors.forename: Hoon
authors.initials: H
authors.lastname: Jeon
authors.forename: Byeong-Suk
authors.initials: BS
authors.lastname: Chae
authors.forename: Jeong-Suk
authors.initials: JS
authors.lastname: Park
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry*; D003189:p-Methoxy-N-methylphenethylamine / Q000009:adverse effects
chemical_list: D018836:Inflammation Mediators; D016328:NF-kappa B; D010936:Plant Extracts; D003189:p-Methoxy-N-methylphenethylamine; D020928:Mitogen-Activated Protein Kinases; D020170:Caspase 1; D002118:Calcium