title: Interconversion between intestinal stem cell populations in distinct niches.
abstract: Intestinal epithelial stem cell identity and location have been the subject of substantial research. Cells in the +4 niche are slow-cycling and label-retaining, whereas a different stem cell niche located at the crypt base is occupied by crypt base columnar (CBC) cells. CBCs are distinct from +4 cells, and the relationship between them is unknown, though both give rise to all intestinal epithelial lineages. We demonstrate that Hopx, an atypical homeobox protein, is a specific marker of +4 cells. Hopx-expressing cells give rise to CBCs and all mature intestinal epithelial lineages. Conversely, CBCs can give rise to +4 Hopx-positive cells. These findings demonstrate a bidirectional lineage relationship between active and quiescent stem cells in their niches.
authors.affiliation: Department of Cell and Developmental Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Takeda
authors.forename: Rajan
authors.initials: R
authors.lastname: Jain
authors.forename: Matthew R
authors.initials: MR
authors.lastname: LeBoeuf
authors.forename: Qiaohong
authors.initials: Q
authors.lastname: Wang
authors.forename: Min Min
authors.initials: MM
authors.lastname: Lu
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Epstein
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects; D007421:Intestine, Small / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008954:Models, Biological; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D019879:Paneth Cells / Q000166:cytology; D055153:Stem Cell Niche; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D018398:Homeodomain Proteins; D013629:Tamoxifen
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