title: Genetic characterization of Escherichia coli O157:H7 strains isolated from the one-humped camel (Camelus dromedarius) by using microarray DNA technology.
abstract: From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Gharib Avenue, Azadi Street 14155-6453, Tehran, Iran.
authors.forename: Taghi Zahraei
authors.initials: TZ
authors.lastname: Salehi
authors.forename: Alfreda
authors.initials: A
authors.lastname: Tonelli
authors.forename: Alberto
authors.initials: A
authors.lastname: Mazza
authors.forename: Hamid
authors.initials: H
authors.lastname: Staji
authors.forename: Pietro
authors.initials: P
authors.lastname: Badagliacca
authors.forename: Iradj Ashrafi
authors.initials: IA
authors.lastname: Tamai
authors.forename: Reza
authors.initials: R
authors.lastname: Jamshidi
authors.forename: Josée
authors.initials: J
authors.lastname: Harel
authors.forename: Rossella
authors.initials: R
authors.lastname: Lelli
authors.forename: Luke
authors.initials: L
authors.lastname: Masson
mesh_terms: D000818:Animals; D002162:Camelus; D004269:DNA, Bacterial / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
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