title: Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation.
abstract: The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet-rich fibrin matrix (PRFM). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet-embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal-regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.
authors.affiliation: Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA.
authors.forename: Sashwati
authors.initials: S
authors.lastname: Roy
authors.forename: Jason
authors.initials: J
authors.lastname: Driggs
authors.forename: Haytham
authors.initials: H
authors.lastname: Elgharably
authors.forename: Sabyasachi
authors.initials: S
authors.lastname: Biswas
authors.forename: Muna
authors.initials: M
authors.lastname: Findley
authors.forename: Savita
authors.initials: S
authors.lastname: Khanna
authors.forename: Urmila
authors.initials: U
authors.lastname: Gnyawali
authors.forename: Valerie K
authors.initials: VK
authors.lastname: Bergdall
authors.forename: Chandan K
authors.initials: CK
authors.lastname: Sen
mesh_terms: D000328:Adult; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000502:physiology*; D034421:Sus scrofa; D014945:Wound Healing / Q000502:physiology*; D055815:Young Adult
chemical_list: D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D005337:Fibrin
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