title: Echovirus 11 infection induces dramatic changes in the actin cytoskeleton of polarized Caco-2 cells.
abstract: Binding of echovirus 11 strain 207 (EV11-207) to Caco-2 monolayers results in rapid transfer of the virus to tight junctions prior to uptake. Using a confocal microscopy based-method, this study quantified the spatiotemporal distribution of actin during the time course of infection by EV11-207 in Caco-2 polarized cells. It was found that binding of EV11-207 to the apical surface resulted in rapid rearrangement of the actin cytoskeleton, concomitant with transport of the virus particles to tight junctions. By interfering with the actin network dynamics, the virus remained trapped at the cell surface, leading to abortion of infection. In addition, it was observed that at 4 h post-infection, concomitant with the detection of virus replication, actin filament was depolymerized and degraded. Finally, it was shown that the mechanisms leading to loss of actin were independent of viral genome synthesis, indicating a potential role for the viral protein synthesis seen in late infection. These data confirmed a previous study on the requirement for an intact actin cytoskeleton for EV11-207 to infect cells and reinforce the notion of actin cytoskeleton subversion by picornaviruses during infection in polarized epithelial cells.
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.|Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.|Department of Clinical Pathology, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland.
authors.forename: Komla
authors.initials: K
authors.lastname: Sobo
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
authors.forename: Amanda D
authors.initials: AD
authors.lastname: Stuart
authors.affiliation: Department of Clinical Pathology, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland.
authors.forename: Laura
authors.initials: L
authors.lastname: Rubbia-Brandt
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
authors.forename: T David K
authors.initials: TDK
authors.lastname: Brown
authors.affiliation: Department of Clinical Pathology, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: McKee
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D018938:Caco-2 Cells; D029822:Enterovirus B, Human / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D014764:Viral Proteins / Q000096:biosynthesis; D053585:Virus Attachment; D014779:Virus Replication
chemical_list: D014764:Viral Proteins