title: Isolation of human nasoseptal chondrogenic cells: a promise for cartilage engineering.
abstract: In cartilaginous tissues, perichondrium cambium layer may be the source of new cartilage. Human nasal septal perichondrium is considered to be a homogeneous structure in which some authors do not recognize the perichondrium internal zone or the cambium layer as a layer distinct from adjacent cartilage surface. In the present study, we isolated a chondrogenic cell population from human nasal septal cartilage surface zone. Nasoseptal chondrogenic cells were positive for surface markers described for mesenchymal stem cells, with exception of CD146, a perivascular cell marker, which is consistent with their avascular niche in cartilage. Although only Sox-9 was constitutively expressed, they also revealed osteogenic and chondrogenic, but not adipogenic, potentials in vitro, suggesting a more restricted lineage potential compared to mesenchymal stem cells. Interestingly, even in absence of chondrogenic growth factors in the pellet culture system, nasoseptal chondrogenic cells had a capacity to synthesize sulfated glycosaminoglycans, large amounts of collagen type II and to a lesser extent collagen type I. The spontaneous chondrogenic potential of this population of cells indicates that they may be a possible source for cartilage tissue engineering. Besides, the pellet culture system using nasoseptal chondrogenic cells may also be a model for studies of chondrogenesis.
authors.affiliation: Programa de Pós-graduação em Ciências Morfológicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. ronaldojfca@yahoo.com.br
authors.forename: Ronaldo J F C
authors.initials: RJ
authors.lastname: do Amaral
authors.forename: Carolina da S G
authors.initials: Cda S
authors.lastname: Pedrosa
authors.forename: Michele C L
authors.initials: MC
authors.lastname: Kochem
authors.forename: Karina R da
authors.initials: KR
authors.lastname: Silva
authors.forename: Marcelo
authors.initials: M
authors.lastname: Aniceto
authors.forename: Cesar
authors.initials: C
authors.lastname: Claudio-da-Silva
authors.forename: Radovan
authors.initials: R
authors.lastname: Borojevic
authors.forename: Leandra S
authors.initials: LS
authors.lastname: Baptista
mesh_terms: D050156:Adipogenesis; D000328:Adult; D002356:Cartilage / Q000502:physiology*; D018929:Cell Culture Techniques; D019070:Cell Lineage; D002469:Cell Separation / Q000379:methods*; D019902:Chondrocytes / Q000166:cytology* / Q000648:ultrastructure; D020219:Chondrogenesis; D006801:Humans; D009300:Nasal Septum / Q000166:cytology* / Q000648:ultrastructure; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods*