title: Competition for cofactor-dependent DNA binding underlies Hox phenotypic suppression.
abstract: Hox transcription factors exhibit an evolutionarily conserved functional hierarchy, termed phenotypic suppression, in which the activity of posterior Hox proteins dominates over more anterior Hox proteins. Using directly regulated Hox targeted reporter genes in Drosophila, we show that posterior Hox proteins suppress the activities of anterior ones by competing for cofactor-dependent DNA binding. Furthermore, we map a motif in the posterior Hox protein Abdominal-A (AbdA) that is required for phenotypic suppression and facilitates cooperative DNA binding with the Hox cofactor Extradenticle (Exd). Together, these results suggest that Hox-specific motifs endow posterior Hox proteins with the ability to dominate over more anterior ones via a cofactor-dependent DNA-binding mechanism.
authors.affiliation: Department of Neuroscience, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Noro
authors.forename: Katherine
authors.initials: K
authors.lastname: Lelli
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.forename: Richard S
authors.initials: RS
authors.lastname: Mann
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C059627:Scr protein, Drosophila; D014157:Transcription Factors; C066591:abd-A protein, Drosophila
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