title: Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas.
abstract: The performance of femtosecond (fs) laser intrastromal ablation was evaluated with backscattering-mode adaptive-optics multiphoton microscopy in ex vivo chicken corneas. The pulse energy of the fs source used for ablation was set to generate two different ablation patterns within the corneal stroma at a certain depth. Intrastromal patterns were imaged with a custom adaptive-optics multiphoton microscope to determine the accuracy of the procedure and verify the outcomes. This study demonstrates the potential of using fs pulses as surgical and monitoring techniques to systematically investigate intratissue ablation. Further refinement of the experimental system by combining both functions into a single fs laser system would be the basis to establish new techniques capable of monitoring corneal surgery without labeling in real-time. Since the backscattering configuration has also been optimized, future in vivo implementations would also be of interest in clinical environments involving corneal ablation procedures.
authors.forename: Emilio J
authors.initials: EJ
authors.lastname: Gualda
authors.forename: Javier R
authors.initials: JR
authors.lastname: Vázquez de Aldana
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez-García
authors.forename: Pablo
authors.initials: P
authors.lastname: Moreno
authors.forename: Juan
authors.initials: J
authors.lastname: Hernández-Toro
authors.forename: Luis
authors.initials: L
authors.lastname: Roso
authors.forename: Pablo
authors.initials: P
authors.lastname: Artal
authors.forename: Juan M
authors.initials: JM
authors.lastname: Bueno
references.citation: Ophthalmology. 2005 Jan;112(1):44-9
references.pmid: 15629819
references.citation: Opt Express. 2008 Oct 13;16(21):16284-93
references.pmid: 18852734
references.citation: Lasers Surg Med. 2007 Sep;39(8):654-8
references.pmid: 17886278
references.citation: Phys Med Biol. 1997 May;42(5):895-912
references.pmid: 9172266
references.citation: Lasers Surg Med. 1990;10(5):463-8
references.pmid: 2233101
references.citation: Exp Eye Res. 2008 May;86(5):835-43
references.pmid: 18395714
references.citation: Exp Eye Res. 2010 Aug;91(2):308-14
references.pmid: 20558159
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2007 Jun;24(6):1562-8
references.pmid: 17491624
references.citation: Invest Ophthalmol Vis Sci. 2008 Dec;49(12):5332-9
references.pmid: 18641284
references.citation: Opt Express. 2008 Mar 17;16(6):4121-9
references.pmid: 18542509
references.citation: Invest Ophthalmol Vis Sci. 1985 Nov;26(11):1455-64
references.pmid: 4055287
references.citation: J Am Intraocul Implant Soc. 1980 Oct;6(4):352-4
references.pmid: 7440377
references.citation: Invest Ophthalmol Vis Sci. 2010 Feb;51(2):850-6
references.pmid: 19815735
references.citation: Invest Ophthalmol Vis Sci. 2011 Jul 18;52(8):5325-31
references.pmid: 21467175
references.citation: J Refract Surg. 1998 Sep-Oct;14(5):541-8
references.pmid: 9791821
references.citation: J Biomed Opt. 2004 Jul-Aug;9(4):760-6
references.pmid: 15250763
references.citation: J Biomed Opt. 2007 Nov-Dec;12(6):064032
references.pmid: 18163848
references.citation: J Biomed Opt. 2010 Nov-Dec;15(6):066004
references.pmid: 21198178
references.citation: J Biomed Opt. 2001 Jul;6(3):277-86
references.pmid: 11516317
references.citation: Graefes Arch Clin Exp Ophthalmol. 2008 Jun;246(6):897-906
references.pmid: 18030488
references.citation: Opt Express. 2010 Nov 8;18(23):24037-47
references.pmid: 21164751
references.citation: Biomed Opt Express. 2011 Jun 1;2(6):1637-48
references.pmid: 21698025
references.citation: Science. 1990 Apr 6;248(4951):73-6
references.pmid: 2321027
references.citation: J Biomed Opt. 2008 May-Jun;13(3):034019
references.pmid: 18601564
references.citation: J Refract Surg. 1997 Nov-Dec;13(7):653-8
references.pmid: 9427203
references.citation: J Refract Surg. 2003 Jan-Feb;19(1):8-14
references.pmid: 12553599
references.citation: J Biomed Opt. 2009 Jul-Aug;14(4):044012
references.pmid: 19725724
references.citation: J Refract Corneal Surg. 1994 Sep-Oct;10(5):498-510
references.pmid: 7530099
references.citation: Arch Ophthalmol. 1974 Jul;92(1):37-41
references.pmid: 4857748
references.citation: Opt Express. 2005 Jul 25;13(15):5791-7
references.pmid: 19498583
references.citation: Ophthalmology. 1984 Sep;91(9):1017-21
references.pmid: 6548556
references.citation: Cell Tissue Res. 2007 Jun;328(3):515-20
references.pmid: 17468893
references.citation: J Biomed Opt. 2010 Mar-Apr;15(2):026007
references.pmid: 20459252
references.citation: Graefes Arch Clin Exp Ophthalmol. 2000 Jan;238(1):33-9
references.pmid: 10664050
references.citation: Ann Anat. 2006 Sep;188(5):395-409
references.pmid: 16999201
references.citation: Cornea. 2011 Jun;30(6):692-701
references.pmid: 21242785
references.citation: Graefes Arch Clin Exp Ophthalmol. 2008 Jul;246(7):1017-20
references.pmid: 18446357
references.citation: Opt Express. 2008 May 26;16(11):7958-68
references.pmid: 18545505
references.citation: Opt Express. 2004 Sep 6;12(18):4275-81
references.pmid: 19483974
references.citation: Opt Express. 2002 Feb 11;10(3):171-6
references.pmid: 19424346
references.citation: Lasers Surg Med. 2007 Jul;39(6):527-33
references.pmid: 17659583
references.citation: Invest Ophthalmol Vis Sci. 2009 Mar;50(3):1198-204
references.pmid: 18836174