title: Following G-quadruplex formation by its intrinsic fluorescence.
abstract: We characterized and compared the fluorescence properties of various well-defined G-quadruplex structures. The increase of intrinsic fluorescence of G-rich DNA sequences when they form G-quadruplexes can be used to monitor the folding and unfolding of G-quadruplexes as a function of cations and temperature. The temperature-dependent fluorescence spectra of different G-quadruplexes also exhibit characteristic patterns. Thus, the stability and possibly also the structure of G-quadruplexes can be characterized and distinguished by their intrinsic fluorescence spectra.
authors.affiliation: Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore.
authors.forename: Nguyen Thuan
authors.initials: NT
authors.lastname: Dao
authors.forename: Reinhard
authors.initials: R
authors.lastname: Haselsberger
authors.forename: Maria-Elisabeth
authors.initials: ME
authors.lastname: Michel-Beyerle
authors.forename: Anh Tuân
authors.initials: AT
authors.lastname: Phan
mesh_terms: D001483:Base Sequence; D043542:DNA, Z-Form / Q000737:chemistry; D054856:G-Quadruplexes; D005872:Giardia / Q000235:genetics; D006801:Humans; D016813:Oxytricha / Q000235:genetics; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D013997:Time Factors
chemical_list: D043542:DNA, Z-Form