title: A ras GTPase-activating protein-binding protein, TaG3BP, associated with the modulation of male fertility in a thermo-sensitive cytoplasmic male sterile wheat line.
abstract: Hybrid wheat development may contribute to higher, more stable yield and could result in greater food security for much of the world's growing population. YS type thermo-sensitive cytoplasmic male sterile (TCMS) wheat lines were developed for use in hybrid wheat breeding in China. To investigate the molecular mechanism of modulation of male fertility in the YS-type TCMS wheat lines, a ras GTPase-activating protein-binding protein (TaG3BP) was examined. The deduced amino acid sequence encoded by TaG3BP was conserved in the sequenced genomes of Embryophyte. TaG3BP expression in the anthers of YS-type TCMS lines taken at the critical fertility reversion stage of pollen development from male fertile anthers was higher than that from male-sterile anthers, either by quantitative real-time PCR or by western blot analysis. Sequence analysis on the cDNA and genomic DNA of TaG3BP in three kinds of K-type CMS wheat lines and their maintainers indicated that there were no significant difference between the genes or in their 5' flanking sequences. The TaG3BP expression revealed by quantitative real-time RT-PCR was lower in the young spikes of these CMS lines than that of their maintainers. This indicates that TaG3BP expression is associated with the modulation, from male-sterile to fertile, of the TCMS wheat line, and TaG3BP might be a key factor in the pathway responsible for the fertility reversion.
authors.affiliation: College of Agronomy, Northwest A & F University, Shaanxi, 712100, Yangling, China. zhoulinlin1219@yahoo.com.cn
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhou
authors.forename: Guoqi
authors.initials: G
authors.lastname: Song
authors.forename: Beiru
authors.initials: B
authors.lastname: He
authors.forename: Yin-Gang
authors.initials: YG
authors.lastname: Hu
mesh_terms: D001947:Breeding; D002352:Carrier Proteins; D003593:Cytoplasm; D005298:Fertility; D035264:Flowers; D018745:Genome, Plant; D006358:Hot Temperature; D007246:Infertility; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000502:physiology*; D017421:Sequence Analysis; D014908:Triticum; D020703:ras GTPase-Activating Proteins / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; D020703:ras GTPase-Activating Proteins
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