title: High glucose promotes pancreatic cancer cell proliferation via the induction of EGF expression and transactivation of EGFR.
abstract: Multiple lines of evidence suggest that a large portion of pancreatic cancer patients suffer from either hyperglycemia or diabetes, both of which are characterized by high blood glucose level. However, the underlying biological mechanism of this phenomenon is largely unknown. In the present study, we demonstrated that the proliferative ability of two human pancreatic cancer cell lines, BxPC-3 and Panc-1, was upregulated by high glucose in a concentration-dependent manner. Furthermore, the promoting effect of high glucose levels on EGF transcription and secretion but not its receptors in these PC cell lines was detected by using an EGF-neutralizing antibody and RT-PCR. In addition, the EGFR transactivation is induced by high glucose levels in concentration- and time-dependent manners in PC cells in the presence of the EGF-neutralizing antibody. These results suggest that high glucose promotes pancreatic cancer cell proliferation via the induction of EGF expression and transactivation of EGFR. Our findings may provide new insight on the links between high glucose level and PC in terms of the molecular mechanism and reveal a novel therapeutic strategy for PC patients who simultaneously suffer from either diabetes or hyperglycemia.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Ma
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guodong
authors.initials: G
authors.lastname: Ma
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhou
authors.forename: Erxi
authors.initials: E
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003920:Diabetes Mellitus; D004815:Epidermal Growth Factor / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D006943:Hyperglycemia; D010190:Pancreatic Neoplasms / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D015533:Transcriptional Activation
chemical_list: D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D005947:Glucose
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