title: [Structures and bioactivity of polysaccharides from isatidis radix].
abstract: OBJECTIVE
  To investigated the chemical structures and bioactivity of polysaccharides from Isatidis Radix.
  
  
  METHODS
  Polysaccharides were extracted and purified by column chromatograph and their chemical structures were identified by UV, IR, NMR, periodic acid oxadation and Smith degradation method and their stimulation effects to macrophage were evaluated by using MTT method.
  
  
  RESULTS
  Five polysaccharides, polysaccharide A , B, C, D and E were gotten and their molecular weights were 2 000, 1 757.1, 1 34 2.7, 955.6, 11.7 kDa, respectively. Polysaccharide A was composed of arabinose, polysaccharide E was composed of arabinose and galactose, polysaccharides B, C, D were composed of glucose and 1 --> 2, 1 --> 3, 1 --> 4, 1 --> 6 linkages existed in polysaccharides A-E, of A, B, C, D, E were alpha-configurations. Polysaccharides B, C and D showed better bioactivity than polysaccharides A and E with stimulation index (SI) of 5.31, 4.76, 5.17.
  
  
  CONCLUSIONS
  Five polysaccharides are seperated firstly from Isatidis Radix.
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210046, China. he_lw@163.com
authors.forename: Liwei
authors.initials: L
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Honglan
authors.initials: H
authors.lastname: Wang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Chen
authors.forename: Dongsong
authors.initials: D
authors.lastname: Sun
authors.forename: Mingyan
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D031219:Isatis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011134:Polysaccharides