title: Investigation of different apple cultivars by high resolution magic angle spinning NMR. A feasibility study.
abstract: (1)H HR-MAS NMR spectroscopy was applied to apple tissue samples deriving from 3 different cultivars. The NMR data were statistically evaluated by analysis of variance (ANOVA), principal component analysis (PCA), and partial least-squares-discriminant analysis (PLS-DA). The intra-apple variability of the compounds was found to be significantly lower than the inter-apple variability within one cultivar. A clear separation of the three different apple cultivars could be obtained by multivariate analysis. Direct comparison of the NMR spectra obtained from apple tissue (with HR-MAS) and juice (with liquid-state HR NMR) showed distinct differences in some metabolites, which are probably due to changes induced by juice preparation. This preliminary study demonstrates the feasibility of (1)H HR-MAS NMR in combination with multivariate analysis as a tool for future chemometric studies applied to intact fruit tissues, e.g. for investigating compositional changes due to physiological disorders, specific growth or storage conditions.
authors.affiliation: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland. martina.vermathen@ioc.unibe.ch
authors.forename: Martina
authors.initials: M
authors.lastname: Vermathen
authors.forename: Mattia
authors.initials: M
authors.lastname: Marzorati
authors.forename: Daniel
authors.initials: D
authors.lastname: Baumgartner
authors.forename: Claudia
authors.initials: C
authors.lastname: Good
authors.forename: Peter
authors.initials: P
authors.lastname: Vermathen
mesh_terms: D001628:Beverages / Q000032:analysis; D005240:Feasibility Studies; D005638:Fruit / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D027845:Malus / Q000737:chemistry* / Q000145:classification*; D015999:Multivariate Analysis