title: Dietary treatments enriched in olive and safflower oils regulate seric and placental matrix metalloproteinases in maternal diabetes.
abstract: OBJECTIVE
  Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development and function, although related to the pro-inflammatory environment when produced in excess. Previous studies have identified MMP-2 and MMP-9 overactivities in the placenta from diabetic rats. In this study, we aimed to determine whether diets supplemented with olive and safflower oil, enriched in natural PPAR ligands, are able to regulate MMP-2 and MMP-9 activities in the placenta and serum from diabetic rats.
  
  
  METHODS
  Diabetes was induced in rat neonates by streptozotocin administration (90mg/kg s.c.). Control and diabetic rats were fed with 6% olive oil- or 6% safflower oil-supplemented diets from days 0.5-13.5 of gestation.
  
  
  METHODS
  On day 13.5 of gestation, placentas and sera were isolated for further determination of matrix metalloproteinases (MMPs) 2 and 9 activities by zymography. Placental MMP-2 and MMP-9 protein concentration and immunolocalization were also determined.
  
  
  RESULTS
  Sera from diabetic pregnant animals showed MMP-2 and MMP-9 overactivities when compared to controls. Serum MMP-9 activity was significantly decreased when the diabetic animals received the olive and safflower oil dietary treatments. Placentas from diabetic rats showed increased MMP-2 and MMP-9 activities and protein concentrations, and both were decreased when diabetic rats received the olive and safflower dietary treatments.
  
  
  CONCLUSIONS
  This study demonstrates that both olive and safflower oil-supplemented diets were able to prevent MMPs overactivities in the placenta from diabetic rats, and that these beneficial effects are reflected in rat sera.
authors.affiliation: Laboratory of Reproduction and Metabolism, CEFyBO-CONICET, School of Medicine, University of Buenos Aires, Paraguay 2155, 17th floor, C1121ABG Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Martinez
authors.forename: M
authors.initials: M
authors.lastname: Sosa
authors.forename: R
authors.initials: R
authors.lastname: Higa
authors.forename: D
authors.initials: D
authors.lastname: Fornes
authors.forename: E
authors.initials: E
authors.lastname: Capobianco
authors.forename: A
authors.initials: A
authors.lastname: Jawerbaum
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D004792:Enzyme Precursors / Q000378:metabolism; D005260:Female; D008024:Ligands; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000378:metabolism*; D000069463:Olive Oil; D047492:Peroxisome Proliferator-Activated Receptors / Q000819:agonists; D010920:Placenta / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010938:Plant Oils / Q000627:therapeutic use*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000378:metabolism; D011254:Pregnancy in Diabetics / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D021381:Protein Transport; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012450:Safflower Oil / Q000627:therapeutic use*; D013311:Streptozocin
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; D004792:Enzyme Precursors; D008024:Ligands; D000069463:Olive Oil; D047492:Peroxisome Proliferator-Activated Receptors; D010938:Plant Oils; D011257:Pregnancy Proteins; D013311:Streptozocin; D012450:Safflower Oil; C120487:pro-matrix metalloproteinase 9; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9