title: [Effect of salvianolic acid B on TGF-beta1-induced human embryonic lung fibroblast's biological behavior].
abstract: OBJECTIVE
  To investigate the effect of salvianolic acid B (SAB) on the proliferation of human embryonic lung fibroblast MRC-5, and the secretion of procollagen I and endogenous transforming growth factor-beta1, (TGF-beta1).
  
  
  METHODS
  The MRC-5 cells were randomly divided into four groups as follows: the control group: cells cultured with DMEM but with no TGF-beta1, or SAB; the TGF-beta1, group: cell cultured with 10 ng/mL TGF-beta1; the SAB1 group: cell cultured with medium with 10 ng/mL TGF-beta1 and 1 pmol/L SAB; the SAB2 group: cell cultured with medium with 10 ng/mL TGF-beta1, and 10 pmol/L SAB. The proliferation of cells was assayed by MTT incorporation. The concentration of amino-terminal propeptide of type I procollagen (PINP), a marker of collagen synthesis, was measured by radioimmunoassay. The endogenous TGF-beta1, levels were measured using ELISA.
  
  
  RESULTS
  The optical density, procollagen I contents, and endogenous TGF-beta1, levels significantly increased when compared with those of the control group (P<0.05). Compared with the TGF-beta1, group, the optical density was obviously lowered, the procollagen I contents and endogenous TGF-beta1, levels significantly decreased in the SAB1 group and the SAB2 group, and better in the SAB2 group, showing statistical difference (P<0.05).
  
  
  CONCLUSIONS
  SAB could inhibit the proliferation of MRC-5 cells induced by TGF-beta1 and attenuate the roles of secreting collagen and endogenous TGF-beta1. It had the potential of postponing or delaying the progressive developing of pulmonary fibrosis.
authors.affiliation: ICU of Guangzhou Red Cross Hospital, Jinan University, Guangzhou. zhangmincc@163.com
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhi-shun
authors.initials: ZS
authors.lastname: Feng
authors.forename: Zhe-tong
authors.initials: ZT
authors.lastname: Deng
mesh_terms: D001572:Benzofurans / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000196:embryology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D053630:Depsides
chemical_list: D001572:Benzofurans; D024042:Collagen Type I; D053773:Transforming Growth Factor beta1; C076944:salvianolic acid B; D053630:Depsides