title: Immunohistochemical localization of hepatopancreatic phospholipase in gastropods mollusc, Littorina littorea and Buccinum undatum digestive cells.
abstract: BACKGROUND
  Among the digestive enzymes, phospholipase A2 (PLA2) hydrolyzes the essential dietary phospholipids in marine fish and shellfish. However, we know little about the organs that produce PLA2, and the ontogeny of the PLA2-cells. Accordingly, accurate localization of PLA2 in marine snails might afford a better understanding permitting the control of the quality and composition of diets and the mode of digestion of lipid food.
  
  
  RESULTS
  We have previously producted an antiserum reacting specifically with mSDPLA2. It labeled zymogen granules of the hepatopancreatic acinar cells and the secretory materials of certain epithelial cells in the depths of epithelial crypts in the hepatopancreas of snail. To confirm this localization a laser capture microdissection was performed targeting stained cells of hepatopancreas tissue sections. A Western blot analysis revealed a strong signal at the expected size (30 kDa), probably corresponding to the PLA2.
  
  
  CONCLUSIONS
  The present results support the presence of two hepatopancreatic intracellular and extracellular PLA2 in the prosobranchs gastropods molluscs, Littorina littorea and Buccinum undatum and bring insights on their localizations.
authors: Laboratoire de biochimie et de génie enzymatique des lipases, ENIS BPW 1173 Université de Sfax-Tunisia.
authors: Zied
authors: Z
authors: Zarai
authors: Nicholas
authors: N
authors: Boulais
authors: Pascale
authors: P
authors: Marcorelles
authors: Eric
authors: E
authors: Gobin
authors: Sofiane
authors: S
authors: Bezzine
authors: Hafedh
authors: H
authors: Mejdoub
authors: Youssef
authors: Y
authors: Gargouri
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D004064:Digestive System / Q000166:cytology / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology* / Q000648:ultrastructure; D005676:Gravity Suits; D043143:Hepatopancreas / Q000166:cytology / Q000201:enzymology*; D007106:Immune Sera / Q000737:chemistry; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D054467:Phospholipases A2 / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D012908:Snails / Q000033:anatomy & histology* / Q000201:enzymology
chemical_list: D007106:Immune Sera; D054467:Phospholipases A2