title: [Establishing the regeneration system of sunflower].
abstract: In order to establish a high efficient regeneration system of sunflower, we optimized the process of callus induction, differentiation and rooting by screening the optimum genotype, explant materials, hormone and cytokine concentration and additives. The results indicated that hybrid sunflowers were easier to regenerate than selfing ones; The best explant was four days cotyledon. The optimum induction medium was Murashige and Skoog (MS) + 2.0 mg/L 6-benzyladenine (6-BA) + 0.5 mg/L naphthaleneacetic acid (NAA) + 1.0 mg/L kinetin (KT). The maximum rate of callus induction was 100%. The optimum differentiation medium was MS +0.2 mg/L 6-BA + 0.5 mg/L NAA + 0.3 mg/L KT + 0.3 mg/L silver nitrate (AgNO3) + 0.2 g/L active carbon (AC), and the buds differentiation rate was up to 71%. The best rooting culture medium was 1/2 MS + 0.6 mg/L indolebutyric acid (IBA). The highest rooting rate was 77%. The analysis of variance showed that genotype, explants growth time, different kinds and concentration of hormone, AC concentration had a significant effect on sunflower regeneration.
authors: College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
authors: Yuanyuan
authors: Y
authors: Wang
authors: Caifeng
authors: C
authors: Li
authors: Yifei
authors: Y
authors: Zhang
authors: Yeting
authors: Y
authors: Chen
authors: Liying
authors: L
authors: Zhao
authors: Peng
authors: P
authors: Yue
authors: Xiangyong
authors: X
authors: Teng
authors: Nanbo
authors: N
authors: Wang
mesh_terms: D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development; D005838:Genotype; D006368:Helianthus / Q000235:genetics / Q000502:physiology*; D006824:Hybridization, Genetic; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D046509:Tissue Culture Techniques
chemical_list: D010937:Plant Growth Regulators