title: Control of the interferon response in RNAi experiments.
abstract: The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever since the discovery of RNAi in mammals, the interferon response has been a feared problem. While RNAi became an efficient and widespread method for gene silencing in mammals, numerous studies recognized several obstacles, including undesirable activation of the interferon response, which need to be overcome to achieve a specific and robust RNAi effect. The aim of this text is to provide theoretical and practical information for scientists who want to control interferon response and other adverse effects in their RNAi experiments.
authors: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic.
authors: Jana
authors: J
authors: Nejepinska
authors: Matyas
authors: M
authors: Flemr
authors: Petr
authors: P
authors: Svoboda
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005786:Gene Expression Regulation; D020868:Gene Silencing; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007372:Interferons / Q000378:metabolism*; D008969:Molecular Sequence Data; D034622:RNA Interference; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D017931:DNA Primers; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D007372:Interferons