title: Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications.
abstract: In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling chemistry. An epoxy-polyurethane based membrane was used to extend the linear working range (from 0 to 25 mM of substrate) of these biosensors. Both lactate and glucose sensors performed well in the clinically relevant substrate concentration range. Glucose microspikes were further investigated with respect to the effects of substrate transfer by incorporation into a microfluidic system. Data from the microfluidic system revealed that the sensor response is mainly dependent on enzyme kinetics rather than membrane permeability to glucose. The robustness of the sensors was demonstrated by its consistency in performance extending over 48 h.
authors: Institute of Biomedical Engineering & Department of Chemistry, Imperial College London, South Kensington Campus, Exhibition Road, London, SW7 2AZ, UK. jtrzebin@imperial.ac.uk
authors: Jakub
authors: J
authors: Trzebinski
authors: Sanjiv
authors: S
authors: Sharma
authors: Anna Radomska-Botelho
authors: AR
authors: Moniz
authors: Kostis
authors: K
authors: Michelakis
authors: Yangyang
authors: Y
authors: Zhang
authors: Anthony E G
authors: AE
authors: Cass
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055664:Electrochemical Techniques; D004853:Epoxy Resins / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D004853:Epoxy Resins; D008567:Membranes, Artificial; D011140:Polyurethanes; D019344:Lactic Acid; C013830:baysilon; D006899:Mixed Function Oxygenases; D005949:Glucose Oxidase; C021497:lactate 2-monooxygenase; D005947:Glucose