title: Analysis of transcriptional regulation in bone cells.
abstract: Transcription is the process by which the rate of RNA synthesis is regulated. Here, we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays, three commonly used methods for studying gene transcription.
authors: Kennedy Institute of Rheumatology, London, UK. h.jin@imperial.ac.uk
authors: Huilin
authors: H
authors: Jin
authors: Stuart H
authors: SH
authors: Ralston
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D024202:Electrophoretic Mobility Shift Assay / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D048790:Renilla / Q000201:enzymology; D015533:Transcriptional Activation
chemical_list: D008156:Luciferases