title: Effects of systemic versus local administration of corticosteroids on mucosal tolerance.
abstract: Respiratory exposure to allergen induces T cell tolerance and protection against the development of airway hyperactivity in animal models of asthma. Whereas systemic administration of dexamethasone during the delivery of respiratory Ag has been suggested to prevent the development of mucosal tolerance, the effects of local administration of corticosteroids, first-line treatment for patients with bronchial asthma, on mucosal tolerance remain unknown. To analyze the effects of systemic versus local administration of different types of corticosteroids on the development of mucosal tolerance, mice were exposed to respiratory allergen to induce mucosal tolerance with or without systemic or intranasal application of different doses of dexamethasone or prednisolone. After the induction of mucosal tolerance, proliferation of T cells was inhibited in tolerized mice, whereas systemic applications of corticosteroids restored T cell proliferation and secretion of Th2 cytokines. In contrast, inhaled corticosteroids showed no effect on both T cell proliferation and cytokine secretion. In addition, mice systemically treated with corticosteroids showed an increased airway hyperactivity with a significant lung inflammation, but also an increased T effector cells/regulatory T cells ratio in the second lymphoid organs when compared with mice that receive corticosteroids by inhalation. These results demonstrate that local administration of corticosteroids has no effect on the development of immune tolerance in contrast to systemically applied corticosteroids. Furthermore, although different concentrations of corticosteroids are administered to patients, our results demonstrated that the route of administration rather than the doses affects the effect of corticosteroids on respiratory tolerance induction. Considering the broad application of corticosteroids in patients with allergic disease and asthma, the route of administration of steroid substances seems crucial in terms of treatment and potential side effects. These findings may help elucidate the apparently contradicting results of corticosteroid treatment in allergic diseases.
authors: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA.
authors: Jerome
authors: J
authors: Kerzerho
authors: Daniela
authors: D
authors: Wunsch
authors: Natacha
authors: N
authors: Szely
authors: Hellmuth-Alexander
authors: HA
authors: Meyer
authors: Lisa
authors: L
authors: Lurz
authors: Lars
authors: L
authors: Röse
authors: Ulrich
authors: U
authors: Wahn
authors: Omid
authors: O
authors: Akbari
authors: Philippe
authors: P
authors: Stock
mesh_terms: D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000188:drug therapy / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects*; D018928:Immunity, Mucosal / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000305:Adrenal Cortex Hormones; D000485:Allergens