title: Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso.
abstract: The study investigated the prevalence of five major Escherichia coli pathogroups in raw meats and beef intestines sold at the local markets in Ouagadougou, Burkina Faso. One hundred and twenty samples (36 beef, 36 beef intestine, 24 mutton and 24 chicken samples) were purchased from four markets between October 2008 and February 2009. Fifteen virulence genes specific for Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC) were examined using 16-plex PCR for mixed bacterial cultures derived from the samples. One or more diarrheagenic E. coli pathogroup was detected in 51 (43%) of all the 120 samples: in 16 (44%) beef, 19 (53%) beef intestine, 9 (38%) mutton and in 7 (29%) chicken samples. Thirty three (28%) samples were positive for stx(1) and/or stx(2) indicating presence of STEC. EPEC virulence markers (eae, escV and/or ent and/or bfp and/or EHEC-hlyA) were detected in 14 (12%) stx-negative samples. ETEC virulence markers (elt and/or estIb and/or estIa) were detected in 10 (8%) samples and EAEC virulence markers (pic or aggR) in 5 (4%) samples. No EIEC was detected. The results show that in Burkina Faso the microbiological quality of retail meat is alarmingly poor due to the common occurrence of diarrheagenic E. coli bacteria.
authors: Bacteriology Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare (THL), PO Box 30, FI-00271 Helsinki, Finland.
authors: Assèta
authors: A
authors: Kagambèga
authors: Outi
authors: O
authors: Martikainen
authors: Taru
authors: T
authors: Lienemann
authors: Anja
authors: A
authors: Siitonen
authors: Alfred S
authors: AS
authors: Traoré
authors: Nicolas
authors: N
authors: Barro
authors: Kaisa
authors: K
authors: Haukka
mesh_terms: D000818:Animals; D002050:Burkina Faso; D002417:Cattle; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D019649:Food Industry / Q000592:standards; D005516:Food Microbiology; D007422:Intestines / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012756:Sheep; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors