title: Electron detachment dissociation of fluorescently labeled sialylated oligosaccharides.
abstract: We explored the application of electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry to fluorescently labeled sialylated oligosaccharides. Standard sialylated oligosaccharides and a sialylated N-linked glycan released from human transferrin were investigated. EDD yielded extensive glycosidic cleavages and cross-ring cleavages in all cases studied, consistently providing complementary structural information compared with infrared multiphoton dissociation. Neutral losses and satellite ions such as C-2H ions were also observed following EDD. In addition, we examined the influence of different fluorescent labels. The acidic label 2-aminobenzoic acid (2-AA) enhanced signal abundance in negative-ion mode. However, few cross-ring fragments were observed for 2-AA-labeled oligosaccharides. The neutral label 2-aminobenzamide (2-AB) resulted in more cross-ring cleavages compared with 2-AA-labeled species, but not as extensive fragmentation as for native oligosaccharides, likely resulting from altered negative charge locations from introduction of the fluorescent tag.
authors: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
authors: Wen
authors: W
authors: Zhou
authors: Kristina
authors: K
authors: Håkansson
mesh_terms: D004583:Electrons; D005456:Fluorescent Dyes / Q000737:chemistry*; D005583:Fourier Analysis; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014168:Transferrin / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009844:Oligosaccharides; D014168:Transferrin; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D019158:N-Acetylneuraminic Acid