title: Simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma using high performance liquid chromatography coupled with tandem mass spectrometric detection: application to a bioequivalence study.
abstract: A rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma has been developed and validated. Following liquid-liquid extraction, the analytes were separated on a reversed-phase C(18) column (150 mm × 2.0 mm, 3 μm) using formic acid:10 mM ammonium acetate:methanol (0.2:62:38, v/v/v) as mobile phase at a flow rate of 0.2 mL/min and analyzed by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode. The method was linear for all analytes over the following concentration (ng/mL) ranges: codeine 0.08-16; ephedrine 0.8-160; guaiphenesin 80-16,000; chlorpheniramine 0.2-40. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. It is the first time that the validated HPLC-MS/MS method was successfully applied to a bioequivalence study in 6 healthy beagle dogs.
authors: Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, China.
authors: Ziyan
authors: Z
authors: Hu
authors: Qiaogen
authors: Q
authors: Zou
authors: Jixin
authors: J
authors: Tian
authors: Lili
authors: L
authors: Sun
authors: Zunjian
authors: Z
authors: Zhang
mesh_terms: D000818:Animals; D002744:Chlorpheniramine / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000097:blood* / Q000493:pharmacokinetics; D004285:Dogs; D004809:Ephedrine / Q000097:blood* / Q000493:pharmacokinetics; D006140:Guaifenesin / Q000032:analysis* / Q000493:pharmacokinetics; D016018:Least-Squares Analysis; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D002744:Chlorpheniramine; D006140:Guaifenesin; D004809:Ephedrine; D003061:Codeine