title: Mussel-inspired surface modification of poly(L-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells.
abstract: Development of biomaterials to control the fate of stem cells is important for stem cell based regeneration of bone tissue. The objective of this study is to develop functionalized electrospun fibers using a mussel-inspired surface coating to regulate adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs). We prepared poly(L-lactide) (PLLA) fibers coated with polydopamine (PD-PLLA). The morphology, chemical composition, and surface properties of fiber were characterized by SEM, AFM, XPS, Raman spectra and water contact angle measurements. Incubation of fibers in dopamine solution for 1h resulted in formation of polydopamine with only negligible effects on the roughness and hydrophobicity of the fibers. However, PD-PLLA fibers modulated hMSC responses in several aspects. Firstly, adhesion and proliferation of hMSCs cultured on PD-PLLA were significantly enhanced relative to those on PLLA. In addition, the ALP activity of hMSCs cultured on PD-PLLA (1.74±0.14 nmole/DNA/30 min) was significantly higher than on PLLA (0.97±0.07 nmole/DNA/30 min). hMSCs cultured on PD-PLLA showed up-regulation of genes associated with osteogenic differentiation as well as angiogenesis. Furthermore, the calcium deposition from hMSCs cultured on PD-PLLA (41.60±1.74 μg) was significantly greater than that on PLLA (30.15±1.21 μg), which was double-confirmed by alizarin red S staining. Our results suggest that the bio-inspired coating synthetic degradable polymer can be used as a simple technique to render the surface of synthetic biodegradable fibers to be active for directing the specific responses of hMSCs.
authors: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea.
authors: Nae Gyune
authors: NG
authors: Rim
authors: Seok Joo
authors: SJ
authors: Kim
authors: Young Min
authors: YM
authors: Shin
authors: Indong
authors: I
authors: Jun
authors: Dong Woo
authors: DW
authors: Lim
authors: Jung Hwan
authors: JH
authors: Park
authors: Heungsoo
authors: H
authors: Shin
mesh_terms: D000818:Animals; D001483:Base Sequence; D049872:Bivalvia; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D017931:DNA Primers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties
chemical_list: D017931:DNA Primers; D011091:Polyesters; C033616:poly(lactide)