title: Nanoresonator chip-based RNA sensor strategy for detection of circulating tumor cells: response using PCA3 as a prostate cancer marker.
abstract: There is widespread interest in circulating tumor cells (CTCs) in blood. Direct detection of CTCs (often < 1/mL) is complicated by a number of factors, but the presence of ∼10(3) to 10(4) copies of target RNA per CTC, coupled with simple enrichments, can greatly increase detection capability. In this study we used resonance frequency shifts induced by mass-amplifying gold nanoparticles to detect a hybridization sandwich bound to functionalized nanowires. We selected PCA3 RNA as a marker for prostate cancer, optimized antisense binding sites, and defined conditions allowing single nucleotide mismatch discrimination, and used a hybrid resonator integration scheme, which combines elements of top-down fabrication with strengths of bottom-up fabrication, with a view to enable multiplexed sensing. Bound mass calculated from frequency shifts matched mass estimated by counting gold nanoparticles. This represents the first demonstration of use of such nanoresonators, which show promise of both excellent specificity and quantitative sensitivity.
authors: Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
authors: James A
authors: JA
authors: Sioss
authors: Rustom B
authors: RB
authors: Bhiladvala
authors: Weihua
authors: W
authors: Pan
authors: Mingwei
authors: M
authors: Li
authors: Susan
authors: S
authors: Patrick
authors: Ping
authors: P
authors: Xin
authors: Stacey L
authors: SL
authors: Dean
authors: Christine D
authors: CD
authors: Keating
authors: Theresa S
authors: TS
authors: Mayer
authors: Gary A
authors: GA
authors: Clawson
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008297:Male; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*; D012313:RNA / Q000235:genetics*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C479341:prostate cancer antigen 3, human; D012313:RNA