title: Role of rabbit prostate granules on sperm viability and acrosome reaction evaluated with different methods.
abstract: In the present study, the role of rabbit seminal granules was observed. Their influence on motility, capacitation and acrosome reaction, as well as the presence of apoptosis and the morphology of rabbit sperm, were compared in different conditions. Ejaculated sperm from five mature New Zealand White rabbit bucks during three series of collections were studied, comparing raw semen, Percoll-selected sperm and Percoll-selected sperm plus prostate granules. We observed sperm motility kinetic traits by computer-assisted sperm analyzer (CASA) analysis in each sample. Acrosome status was evaluated by FITC-labeled Pisum sativum Agglutinin staining and chlortetracycline fluorescence assay, phosphatidylserine translocation was determined by AnnexinV/Propidium iodide assay and sperm morphology was studied using transmission electron microscopy (TEM). All traits were observed after 30 min incubation at 37 °C in 5% CO(2). Data showed that sperm motility and viability markedly improved in the presence of prostate granules, whereas capacitation, acrosome reaction and phosphatidylserine translocation were lowered. TEM confirmed these results. In conclusion, the role of granules was confirmed in synchronizing sperm capacitation and acrosome reaction with egg availability; indeed, rabbit ovulation occurs only 6 to 10 h after mating.
authors: Department of Biomedical Sciences, Applied Biology Section, University of Siena, Ospedale Le Scotte, 53100 Siena, Italy. giulia.collodel@unisi.it
authors: Giulia
authors: G
authors: Collodel
authors: Raffaella
authors: R
authors: Cardinali
authors: Elena
authors: E
authors: Moretti
authors: Simona
authors: S
authors: Mattioli
authors: Silvia
authors: S
authors: Ruggeri
authors: Cesare
authors: C
authors: Castellini
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000502:physiology*; D008297:Male; D046529:Microscopy, Electron, Transmission; D011467:Prostate / Q000648:ultrastructure*; D011817:Rabbits; D012661:Semen; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*