title: Re-programming DNA-binding specificity in zinc finger proteins for targeting unique address in a genome.
abstract: Recent studies provide a glimpse of future potential therapeutic applications of custom-designed zinc finger proteins in achieving highly specific genomic manipulation. Custom-design of zinc finger proteins with tailor-made specificity is currently limited by the availability of information on recognition helices for all possible DNA targets. However, recent advances suggest that a combination of design and selection method is best suited to identify custom zinc finger DNA-binding proteins for known genome target sites. Design of functionally self-contained zinc finger proteins can be achieved by (a) modular protein engineering and (b) computational prediction. Here, we explore the novel functionality obtained by engineered zinc finger proteins and the computational approaches for prediction of recognition helices of zinc finger proteins that can raise our ability to re-program zinc finger proteins with desired novel DNA-binding specificities.
authors: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology (IIT) Delhi, Hauz Khas New Delhi, 110016 India.
authors: Abhinav
authors: A
authors: Grover
authors: Akshay
authors: A
authors: Pande
authors: Krishna
authors: K
authors: Choudhary
authors: Kriti
authors: K
authors: Gupta
authors: Durai
authors: D
authors: Sundar