title: Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
abstract: We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.
authors: Department of Chemistry and Biochemistry, University of California, Santa Barbara, USA.
authors: Xiaolei
authors: X
authors: Zuo
authors: Fan
authors: F
authors: Xia
authors: Adriana
authors: A
authors: Patterson
authors: H Tom
authors: HT
authors: Soh
authors: Yi
authors: Y
authors: Xiao
authors: Kevin W
authors: KW
authors: Plaxco
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015342:DNA Probes; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D016133:Polymerase Chain Reaction / Q000379:methods*; D019098:Telomerase / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D019098:Telomerase; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III