title: Effects of GABA ligands injected into the nucleus accumbens shell on fear/anxiety-like and feeding behaviours in food-deprived rats.
abstract: In an attempt to establish a relationship between food intake and fear/anxiety-related behaviours, the goal of this study was to investigate the effect of bilateral injections of GABAA (Muscimol, MUS, doses 25 and 50ng/side) and GABAB (Baclofen, BAC, doses 32 and 64ng/side) receptor agonists in the nucleus accumbens shell (AcbSh) on the level of fear/anxiety-like and feeding behaviours in 24h food-deprived rats. The antagonists of GABAA (Bicuculline, BIC, doses 75 and 150ng/side) and GABAB (Saclofen, SAC, doses 1.5 and 3μg/side) were also tested. The results indicated that the total number of risk assessment behaviour decreased after the injection of both doses of GABAA agonist (MUS) into the AcbSh of 24h food-deprived rats exposed to elevated plus maze. Similar results were obtained after treatment with both doses of GABAB (BAC) agonist in the AcbSh. These data indicated that the activation of both GABAA and GABAB receptors within the AcbSh caused anxiolysis in 24h food-deprived rats. In addition, feeding behaviour (food intake, feeding latency and feeding duration) remained unchanged after treatment with both GABA agonists. In contrast, both food intake and feeding duration decreased after injections of both doses of BIC (GABAA antagonist), while the feeding latency remained unchanged after treatment with both GABA antagonists in the AcbSh of 24h food-deprived rats. The treatment with SAC (GABAB antagonist) did not affect feeding behaviour. Collectively, these data suggest that emotional changes evoked by pharmacological manipulation of the GABA neurotransmission in the AcbSh are not linked with changes in food intake.
authors: Department of Physiological Sciences, Centre of Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil. anapfragalopes@gmail.com, marta@ccb.ufsc.br
authors: Ana Paula Fraga
authors: AP
authors: Lopes
authors: Laís
authors: L
authors: Ganzer
authors: Aline Caon
authors: AC
authors: Borges
authors: Larissa
authors: L
authors: Kochenborger
authors: Ana Cláudia
authors: AC
authors: Januário
authors: Moacir Serralvo
authors: MS
authors: Faria
authors: José
authors: J
authors: Marino-Neto
authors: Marta Aparecida
authors: MA
authors: Paschoalini
mesh_terms: D000818:Animals; D001007:Anxiety / Q000523:psychology*; D001418:Baclofen / Q000031:analogs & derivatives / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001640:Bicuculline / Q000494:pharmacology; D004435:Eating / Q000187:drug effects; D005239:Fear / Q000187:drug effects / Q000523:psychology*; D005247:Feeding Behavior / Q000187:drug effects*; D005508:Food Deprivation / Q000502:physiology*; D018682:GABA Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D058785:GABA-A Receptor Agonists / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D058786:GABA-B Receptor Agonists / Q000494:pharmacology; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D009118:Muscimol / Q000494:pharmacology; D009714:Nucleus Accumbens / Q000033:anatomy & histology; D051381:Rats; D017208:Rats, Wistar; D013238:Stereotaxic Techniques
chemical_list: D018682:GABA Agents; D018755:GABA Agonists; D018756:GABA Antagonists; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D058786:GABA-B Receptor Agonists; D058788:GABA-B Receptor Antagonists; D009118:Muscimol; D001418:Baclofen; C080629:saclofen; D001640:Bicuculline