title: De novo expression of podocyte proteins in parietal epithelial cells in experimental aging nephropathy.
abstract: A progressive decrease in podocyte number underlies the development of glomerulosclerosis and reduced kidney function in aging nephropathy. Recent data suggest that under certain disease states, parietal epithelial cells (PECs) begin to express proteins considered specific to podocytes. To determine whether this phenomenon increases in aging kidneys, 4-, 12-, and 20-mo ad libitum-fed and 20-mo calorie-restricted (CR) rats were studied. Single and double immunostaining were performed with antibodies to the PEC protein paired box gene 2 (PAX2) and tight junction protein claudin-1, the podocyte-specific protein Wilms' tumor 1 (WT-1), and the proliferating cell protein (Ki-67). ImageJ software measured Bowman's basement membrane (BBM) length and glomerular tuft area in individual glomeruli from each animal to assess glomerular size. The results showed that in aged ad libitum rats, the decrease in number of podocytes/glomerular tuft area was accompanied by an increase in the number of PECs/BBM length at 12 and 20 mo (P < 0.01 vs. 4 mo). The increase in PEC number was due to proliferation (increase in PAX2/Ki-67 double-positive cells). Aging was accompanied by a progressive increase in the number of glomerular cells double staining for PAX2 and WT-1. In contrast, the control 20-mo-old CR rats had no increase in glomerular size, and podocyte and PEC number were not altered. These results suggest that although the number of PECs and PECs expressing podocyte proteins increase in aging nephropathy, they are likely not sufficient to compensate for the decrease in podocyte number.
authors: Div. of Nephrology, Dept. of Medicine, Univ. of Washington School of Medicine, Seattle, WA 98195-6521, USA.
authors: Jiong
authors: J
authors: Zhang
authors: Kim M
authors: KM
authors: Hansen
authors: Jeffrey W
authors: JW
authors: Pippin
authors: Alice M
authors: AM
authors: Chang
authors: Yoshinori
authors: Y
authors: Taniguchi
authors: Ronald D
authors: RD
authors: Krofft
authors: Scott G
authors: SG
authors: Pickering
authors: Zhi-Hong
authors: ZH
authors: Liu
authors: Christine K
authors: CK
authors: Abrass
authors: Stuart J
authors: SJ
authors: Shankland
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D049109:Cell Proliferation; D062445:Claudin-1; D019394:Ki-67 Antigen / Q000378:metabolism; D007674:Kidney Diseases / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051762:PAX2 Transcription Factor / Q000378:metabolism; D050199:Podocytes / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D025721:WT1 Proteins / Q000378:metabolism
chemical_list: D062445:Claudin-1; C568114:Cldn1 protein, rat; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D051762:PAX2 Transcription Factor; C551986:PAX2 protein, rat; D025721:WT1 Proteins