title: Application of Spiroplasma melliferum proteogenomic profiling for the discovery of virulence factors and pathogenicity mechanisms in host-associated spiroplasmas.
abstract: To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.
authors: Russian Institute of Physico-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow, Russian Federation. alexeev@niifhm.ru
authors: Dmitry
authors: D
authors: Alexeev
authors: Elena
authors: E
authors: Kostrjukova
authors: Alexander
authors: A
authors: Aliper
authors: Anna
authors: A
authors: Popenko
authors: Nikolay
authors: N
authors: Bazaleev
authors: Alexander
authors: A
authors: Tyakht
authors: Oksana
authors: O
authors: Selezneva
authors: Tatyana
authors: T
authors: Akopian
authors: Elena
authors: E
authors: Prichodko
authors: Ilya
authors: I
authors: Kondratov
authors: Mikhail
authors: M
authors: Chukin
authors: Irina
authors: I
authors: Demina
authors: Maria
authors: M
authors: Galyamina
authors: Dmitri
authors: D
authors: Kamashev
authors: Anna
authors: A
authors: Vanyushkina
authors: Valentina
authors: V
authors: Ladygina
authors: Sergei
authors: S
authors: Levitskii
authors: Vasily
authors: V
authors: Lazarev
authors: Vadim
authors: V
authors: Govorun
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003062:Codon; D016680:Genome, Bacterial; D054884:Host-Pathogen Interactions; D007313:Insecta / Q000382:microbiology; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010449:Peptide Mapping; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013149:Spiroplasma / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003062:Codon; D020543:Proteome; D037521:Virulence Factors