title: Electrochemical detection of DNA hybridization using metallacarborane unit.
abstract: The evaluation of novel electrochemically active label for electrochemical detection of DNA hybridization is presented. Metallacarborane units modified with iron, cobalt or chromium were investigated. The value of redox potential and relatively strong current signal facilitate usage of Fe-carborane as marker covalently attached to the ssDNA. In electrochemical genosensor the sequence complementary to UL55 gene was labeled and used as a target for biosensor device. Interactions were investigated using electrochemical and piezoelectric methods. Obtained results confirm usefulness of the designed label in electrochemical detection of DNA hybridization.
authors: Warsaw University of Technology, Faculty of Chemistry, Institute of Biotechnology, Department of Microbioanalytics, Warsaw, Poland. rziolkowski@ch.pw.edu.pl
authors: Robert
authors: R
authors: Ziółkowski
authors: Agnieszka B
authors: AB
authors: Olejniczak
authors: Łukasz
authors: Ł
authors: Górski
authors: Justyna
authors: J
authors: Janusik
authors: Zbigniew J
authors: ZJ
authors: Leśnikowski
authors: Elżbieta
authors: E
authors: Malinowska
mesh_terms: D001880:Boranes / Q000737:chemistry*; D002857:Chromium / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000032:analysis*; D055664:Electrochemical Techniques; D005814:Genes, Viral; D006801:Humans; D007501:Iron / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D001880:Boranes; D015342:DNA Probes; D004277:DNA, Single-Stranded; C546870:UL55 protein, human cytomegalovirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D002857:Chromium; D003035:Cobalt; D007501:Iron