title: [Effect of hypoxia on the proliferation and hypoxia inducible factor-1α expression in human leukemia HL-60 cells].
abstract: OBJECTIVE
  To explore the effects of hypoxia on the proliferation of human leukemia HL-60 cells and the cellular expression of hypoxia inducible factor-1α (HIF-1α).
  
  
  METHODS
  Human acute myeloid leukemia HL-60 cells with exponential growth in routine culture were exposed to 50, 200, 400, 800 µmol/L CoCl(2) to mimic hypoxic conditions. At 24, 48, and 72 h, the cells were collected for morphological observation, MTT assay, and real-time quantitative PCR for HIF-1α mRNA expression.
  
  
  RESULTS
  Compared with the cells without CoCl(2) treatment, the cells with CoCl(2) exposure exhibited obvious morphological changes and a significant growth inhibition which increased with CoCl(2)concentration and exposure time. At low concentrations (50-200 µmol/L), CoCl(2) treatment caused a dose- and time-dependent enhancement of HIF-1α expression in HL-60 cells.
  
  
  CONCLUSIONS
  Hypoxia mimicked by CoCl(2) exposure significantly inhibits the proliferation of HL-60 cells, and at the non-toxic doses, CoCl(2) dose- and time-dependently increases the expression of HIF-1α. The mimicked hypoxic conditions do not cause differentiation of HL-60 cells.
authors: Department of Clinical Laboratory Medicine, Guiyang Medical College, Guiyang 550004, China. zhangyl20011002@yahoo.com.cn
authors: Ya-li
authors: YL
authors: Zhang
authors: Lin
authors: L
authors: Xu
authors: Jian
authors: J
authors: Qiu
authors: Zhi-liang
authors: ZL
authors: Li
authors: Jian-qing
authors: JQ
authors: Wang
authors: Rui
authors: R
authors: Li
authors: Hui
authors: H
authors: Liu
authors: Hong-min
authors: HM
authors: Zhu
mesh_terms: D015687:Cell Hypoxia; D049109:Cell Proliferation; D003035:Cobalt / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D003035:Cobalt; C018021:cobaltous chloride