title: Expression analysis of the type I keratin protein keratin 33A in goat coat hair.
abstract: The coat of a goat, like that of many mammalian species, consists of an outer coat of coarse hairs and an under coat of fine, downy hairs. The coarse guard hairs are produced by primary follicles and the finer cashmere hairs by secondary follicles. We previously reported that hair keratins are components of cashmere hair, and proteomic analysis revealed that their expression is elevated in winter coat hair. To determine detailed characterization, we have cloned keratin 33A gene, a major highly expressed keratin in winter, and then analyzed the expression of goat hair coat. By Western analysis, we detected that keratin 33A protein is expressed only in hair coat among the various goat tissues. Moreover, the expression level in winter has increased in cashmere high-producing Korean native breed, whereas the expression levels between summer and winter had not changed in cashmere low-producing Saanen. In addition, by immunohistochemistry we determined that keratin 33A is localized in the major cortex portion of cashmere fiber. These results confirm that keratin 33A is a structural protein of goat cashmere hair fiber.
authors: Department of Bioproduction, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
authors: Yuta
authors: Y
authors: Seki
authors: Michinari
authors: M
authors: Yokohama
authors: Kenta
authors: K
authors: Wada
authors: Masaru
authors: M
authors: Fujita
authors: Mai
authors: M
authors: Kotani
authors: Yoshio
authors: Y
authors: Nagura
authors: Masako
authors: M
authors: Kanno
authors: Kou
authors: K
authors: Nomura
authors: Takashi
authors: T
authors: Amano
authors: Yoshiaki
authors: Y
authors: Kikkawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D006041:Goats / Q000235:genetics*; D006197:Hair / Q000737:chemistry*; D007150:Immunohistochemistry; D053530:Keratins, Type I / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D012756:Sheep
chemical_list: D053530:Keratins, Type I