title: Transfection of osteoclasts and osteoclast precursors.
abstract: Osteoclasts and their precursors have traditionally been considered difficult cells to transfect using standard approaches. Here, we describe several methods for transfection of mature osteoclasts and their precursors using the Amaxa™ Nucleofector system, lentiviruses, and adenoviruses.
authors: Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK. j.c.crockett@abdn.ac.uk
authors: Julie C
authors: JC
authors: Crockett
authors: David J
authors: DJ
authors: Mellis
authors: Adam
authors: A
authors: Taylor
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D018274:Electroporation / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods; D014162:Transfection