title: Transglutaminase-catalyzed preparation of human elastin-like polypeptide-based three-dimensional matrices for cell encapsulation.
abstract: Elastin is a specialized component of the extracellular matrix and constitutes an interesting model for bio-inspired material design with a high potential for applications in biomedical and biotechnological fields. In this work, we have focused on the set up of a simple, enzymatic method for the preparation of three-dimensional matrices based on an human elastin-like biomimetic polypeptide. The matrices were described and assayed as culture substrate for cell seeding and growth. An advantage of the here-presented method is the use of mild conditions that preserve cell viability. This can be a powerful tool for many applications that require direct embedding of cells in the matrix.
authors: Dipartimento di Scienze della Vita, Università degli Studi di Trieste, via Giorgieri, 1, 34127 Trieste, Italy. abandiera@units.it
authors: Antonella
authors: A
authors: Bandiera
mesh_terms: D001709:Biotechnology; D002384:Catalysis; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003432:Cross-Linking Reagents; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*; D011503:Transglutaminases / Q000378:metabolism*; D033921:Vesicular Transport Proteins
chemical_list: D003432:Cross-Linking Reagents; C475146:MCFD2 protein, human; D010455:Peptides; D033921:Vesicular Transport Proteins; D004549:Elastin; D011503:Transglutaminases