title: [Use of procalcitonine in intensive care units: comparison of semi quantitative PCT-Q Brahms assay with automated PCT-Kryptor assay].
abstract: Procalcitonine (PCT) is recognized as a major and specific biomarker in diagnosis of bacterial infection. Used early in sepsis, it allows immediate administration of antibiotics and monitoring its effectiveness. Confronted on systemic inflammation response syndrom (SIRS), physicians must react quickly and effectively to evaluate bacterial infection and sepsis. The objective of this study was to compare analytical and clinical performances of semi-quantitative PCT-Q assay (Brahms) with quantitative and automated assay such on Kryptor (Brahms). Fifty blood samples of intensive care patients were compared. The analytical performance observed with PCT-Q assay is accurate: linear ratio kappa of 0.912 (95% CI 0.61, 0.97) and a good correlation between these techniques (p < 0.0001) (MedCalc software) were observed. Three discordances were observed and confirm the difficulties of reading for values close to 0.5 ng/mL. For these patients, PCT result showed its interest to discriminate local infection of a sepsis, to stop antibiotherapy with broad spectrum and to consolidate a therapeutic effectiveness in multi-visceral failure context. The semi-quantitative assay seems adapted for a fast and reliable evaluation of PCT in a general-purpose laboratory, not requiring neither dedicated analyzer, nor complex technicality but a control of the visual evaluation of results. It could be used for diagnosis of sepsis without monitoring precisely therapeutic follow-up.
authors: Laboratoire de biologie médicale.
authors: Géraldine
authors: G
authors: Schuch
authors: Catherine
authors: C
authors: Duc-Marchand
authors: Cyrille
authors: C
authors: Venet
authors: Hubert
authors: H
authors: Mann
authors: Anne
authors: A
authors: Tixier
authors: Clara
authors: C
authors: Bionda
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation; D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D002116:Calcitonin / Q000032:analysis* / Q000097:blood; D003124:Colorimetry / Q000379:methods; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data*; D006801:Humans; D007362:Intensive Care Units / Q000706:statistics & numerical data; D057230:Limit of Detection; D011498:Protein Precursors / Q000032:analysis* / Q000097:blood; D012189:Retrospective Studies; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000175:diagnosis; D014552:Urinary Tract Infections / Q000097:blood / Q000175:diagnosis; D054928:Validation Studies as Topic
chemical_list: D011498:Protein Precursors; D002116:Calcitonin