title: Generation of scFv phages specific to Staphylococcus enterotoxin C1 by panning on related antigens.
abstract: A method for generation of highly specific miniantibodies within the phage particle has been developed, and used to produce antibodies against Staphylococcus enterotoxin type C1. Under successive panning of the non-immune phage miniantibody (scFv) library with enterotoxins SE (types A, B, C1, D, E, G, and I) adsorbed on the plate surface, we generated 11 individual phage clones to Staphylococcus enterotoxin type C1. Five of them interacted specifically only with SEC1 and had no cross-reactions with the other enterotoxins.
authors: Pushchino Branch of Shemyakin-Ovchinnikov Institute of Biororganic Chemistry, Moscow, Russia.
authors: Anna V
authors: AV
authors: Lomonosova
authors: Alexander G
authors: AG
authors: Laman
authors: Ksenia K
authors: KK
authors: Fursova
authors: Anna O
authors: AO
authors: Shepelyakovskaya
authors: Yury V
authors: YV
authors: Vertiev
authors: Fedor A
authors: FA
authors: Brovko
authors: Evgeny V
authors: EV
authors: Grishin
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D017104:Bacteriophage M13 / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D004768:Enterotoxins / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D019151:Peptide Library; D057127:Single-Chain Antibodies; C034959:enterotoxin C, staphylococcal