title: The response to rapid infusion of fentanyl in the human brain measured using pulsed arterial spin labelling.
abstract: OBJECTIVE
  We evaluated the sensitivity of pulsed Arterial Spin Labelling (pASL) for the detection of changes in regional cerebral blood perfusion (CBP) during and after intra-venous (i.v.) infusion of an opioid agonist (fentanyl) and an opioid antagonist (naloxone).
  
  
  METHODS
  Twenty-three subjects were scanned four times, receiving i.v. infusion of fentanyl, naloxone, placebo and a second fentanyl administration, in four separate scanning sessions in randomised order. End-tidal CO(2), respiration rate and heart rate were recorded continuously throughout each scan. pASL time series were collected using single shot EPI for 15 min (including 5 min of baseline prior to infusion).
  
  
  RESULTS
  Significant increases in CBP were detected during and after administration of fentanyl, (when compared to placebo and naloxone), in most areas of high concentration of mu-opioid receptors (thalamus, lingual gyrus, para-hippocampal gyrus, and insula); near-significant increases were also observed in the insula. No increases in perfusion were observed during or after naloxone infusion. No correlation was found between regional rCBF changes and end-tidal CO(2), respiration rate or heart rate. Good reliability was found between the first and second fentanyl sessions but the regions of high reliability did not overlap completely with those of highest perfusion change.
  
  
  CONCLUSIONS
  pASL is a suitable method for examining rapid, dynamic effects of opioid administration on brain physiology.
authors: Department of Neuroimaging, Centre for Neuroimaging Sciences, Institute of Psychiatry, King's College London, De Crespigny Park, London SE5 8AF, UK. fernando.zelaya@kcl.ac.uk
authors: Fernando O
authors: FO
authors: Zelaya
authors: Evangelos
authors: E
authors: Zois
authors: Christopher
authors: C
authors: Muller-Pollard
authors: David J
authors: DJ
authors: Lythgoe
authors: Sarah
authors: S
authors: Lee
authors: Caroline
authors: C
authors: Andrews
authors: Trevor
authors: T
authors: Smart
authors: Patricia
authors: P
authors: Conrod
authors: William
authors: W
authors: Vennart
authors: Steven C R
authors: SC
authors: Williams
authors: Mitul A
authors: MA
authors: Mehta
authors: Laurence J
authors: LJ
authors: Reed
mesh_terms: D000328:Adult; D000701:Analgesics, Opioid / Q000008:administration & dosage; D001921:Brain / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology; D002536:Cerebral Arteries / Q000187:drug effects / Q000502:physiology; D002560:Cerebrovascular Circulation; D017352:Echo-Planar Imaging / Q000379:methods*; D005283:Fentanyl / Q000008:administration & dosage*; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D009270:Naloxone / Q000008:administration & dosage; D055420:Perfusion Imaging / Q000379:methods; D012039:Regional Blood Flow / Q000502:physiology*; D015203:Reproducibility of Results; D013113:Spin Labels
chemical_list: D000701:Analgesics, Opioid; D013113:Spin Labels; D009270:Naloxone; D005283:Fentanyl