title: [Screening and identification of marine fungi against bacterial quorum sensing].
abstract: The discovery of quorum sensing (QS) system and its critical role in bacterial virulence have revealed a new way to attack pathogenic bacterium. The pathogenecity of QS deletion mutants decreases significantly. Targeting bacterial QS system is a promising therapeutic approach to control infections and anti-microbial resistance. To obtain natural QS inhibitors from marine organisms, marine fungi (69 strains) were isolated from marine mollusca, and their extracts were screened using improved QSIS2 (Quorum Sensing Inhibitor Selector 2) assay and Chromobacterium violaceum CV026. To improve the efficiency of QSIS2 screening, 2,3,5-triphenyltetrazolium chloride (TTC) staining method was used. Extract from strain QY013 was found to have QS inhibitory activity. Further experiment indicated that pyocyanin in Pseudomonas aeruginosa PAOI and violacein in C. violaceum CV026 were reduced by QY013 extract, without affecting bacterial growth. Morphological and 18S rDNA sequence analysis revealed that strain QY013 was most closely related to Penicillium species. The above results suggest that active constituents from QY013 may be used as novel antimicrobial agents against bacterial infection.
authors: Key Laboratory of Marine Drugs, Ministry of Education, College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China.
authors: Shouliang
authors: S
authors: Yin
authors: Yajing
authors: Y
authors: Chang
authors: Suping
authors: S
authors: Deng
authors: Qingchi
authors: Q
authors: Wang
authors: Wengong
authors: W
authors: Yu
authors: Qianhong
authors: Q
authors: Gong
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D018407:Bacterial Physiological Phenomena; D005658:Fungi / Q000302:isolation & purification / Q000502:physiology; D008386:Marine Biology; D008974:Mollusca / Q000382:microbiology; D010407:Penicillium / Q000302:isolation & purification* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity; D053038:Quorum Sensing / Q000187:drug effects*; D014774:Virulence / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents