title: The effects of oral consumption of selenium nanoparticles on chemotactic and respiratory burst activities of neutrophils in comparison with sodium selenite in sheep.
abstract: The present study was designed to compare the effects of nano-selenium and of sodium selenite on the chemotactic and respiratory burst activities of neutrophils in sheep. Fifteen sheep were randomly divided into three groups. Groups 1 and 2 received selenium nanoparticles (1 mg/kg) or sodium selenite (1 mg/kg) orally, respectively, for ten consecutive days, and the third group was considered as the control. To determine the chemotactic and respiratory burst activities of the neutrophils, the leading front assay and the NBT test were used on heparinized blood samples that were collected at different intervals (days 0, 10th, 20th, and 30th). The results obtained showed that the chemotactic activities in groups 1 and 2 increased significantly on the 10th, 20th, and 30th day, compared to day 0, and on the 20th day in comparison with the 10th day, while in group 2, there was a significant decrease on the 30th day compared to the 20th day. The chemotactic activities in group 1 were significantly higher than in group 2 on the 10th day and in the control group on the 10th, 20th, and 30th day, but the chemotactic activities in group 2 were significantly higher than those in the control group only on the 20th day. On the 30th day into the experiment, the respiratory bursts in groups 1 and 2 were significantly stronger in comparison with those at day 0. Overall, nano-selenium increased the chemotactic and respiratory burst activities more significantly than sodium selenite, which is suggestive of a stronger stimulatory effect of the Se nanoparticles on intracellular activities.
authors: Department of Clinical Science, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
authors: Gholam Ali
authors: GA
authors: Kojouri
authors: Sirous
authors: S
authors: Sadeghian
authors: Abdonnaser
authors: A
authors: Mohebbi
authors: Mohammad Reza
authors: MR
authors: Mokhber Dezfouli
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D053758:Nanoparticles; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011897:Random Allocation; D016897:Respiratory Burst / Q000187:drug effects*; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D012756:Sheep; D018038:Sodium Selenite / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D012643:Selenium; D018038:Sodium Selenite