title: Rv2607 from Mycobacterium tuberculosis is a pyridoxine 5'-phosphate oxidase with unusual substrate specificity.
abstract: Despite intensive effort, the majority of the annotated Mycobacterium tuberculosis genome consists of genes encoding proteins of unknown or poorly understood function. For example, there are seven conserved hypothetical proteins annotated as homologs of pyridoxine 5'-phosphate oxidase (PNPOx), an enzyme that oxidizes pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) to form pyridoxal 5'-phosphate (PLP). We have characterized the function of Rv2607 from Mycobacterium tuberculosis H37Rv and shown that it encodes a PNPOx that oxidizes PNP to PLP. The k(cat) and K(M) for this reaction were 0.01 s(-1) and 360 µM, respectively. Unlike many PNPOx enzymes, Rv2607 does not recognize PMP as a substrate.
authors: National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland, United States of America.
authors: Ellene H
authors: EH
authors: Mashalidis
authors: Tathagata
authors: T
authors: Mukherjee
authors: Paweł
authors: P
authors: Sledź
authors: Dijana
authors: D
authors: Matak-Vinković
authors: Helena
authors: H
authors: Boshoff
authors: Chris
authors: C
authors: Abell
authors: Clifton E
authors: CE
authors: Barry
mesh_terms: D005486:Flavin Mononucleotide / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D010084:Oxidation-Reduction; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000378:metabolism; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011732:Pyridoxal Phosphate; D005486:Flavin Mononucleotide; D011734:Pyridoxaminephosphate Oxidase; C024593:pyridoxine 5-phosphate