title: Urinary stone analysis in a patient with hyperuricemia to determine the mechanism of stone formation.
abstract: In order to determine the mechanism of urinary stone formation in patients with hyperuricemia, we analyzed the crystal components and matrix proteins in a urinary stone from such a patient. Micro-area X-ray spectrometry and infrared (IR) spectroscopy suggested that the outside of the stone was composed of calcium oxalate monohydrate (COM) and the inside of uric acid (UA). Proteomic analysis identified 37 and 14 proteins from the inside and outside of the stone, respectively, as matrix proteins. The proteins that were identified in an ethylenediaminetetraacetic acid (EDTA) fraction were able to bind calcium ions. Thus, calcium-binding proteins may play a significant role in the formation of urinary stones in patients with hyperuricemia.
authors: Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. kikaneko@pharm.teikyo-u.ac.jp
authors: Kiyoko
authors: K
authors: Kaneko
authors: Norifumi
authors: N
authors: Yoshida
authors: Keitaro
authors: K
authors: Okazaki
authors: Tomoyo
authors: T
authors: Yamanobe
authors: Noriko
authors: N
authors: Yamaoka
authors: Makoto
authors: M
authors: Yasuda
authors: Nobuyuki
authors: N
authors: Ogata
authors: Yasukazu
authors: Y
authors: Yamada
authors: Shunya
authors: S
authors: Uchida
authors: Shin
authors: S
authors: Fujimori
mesh_terms: D000293:Adolescent; D000328:Adult; D006801:Humans; D033461:Hyperuricemia / Q000150:complications*; D008297:Male; D013055:Spectrophotometry, Infrared; D014527:Uric Acid / Q000032:analysis; D014545:Urinary Calculi / Q000150:complications*; D014961:X-Ray Diffraction; D055815:Young Adult
chemical_list: D014527:Uric Acid