title: Mechanical stimulation of bone cells using fluid flow.
abstract: This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
authors: Department of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, UK. carmen.huesa@roslin.ed.ac.uk
authors: Carmen
authors: C
authors: Huesa
authors: Astrid D
authors: AD
authors: Bakker
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004867:Equipment Design; D006801:Humans; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009569:Nitric Oxide / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification; D011673:Pulsatile Flow; D012313:RNA / Q000302:isolation & purification; D013314:Stress, Mechanical; D013312:Stress, Physiological
chemical_list: D011506:Proteins; D009569:Nitric Oxide; D012313:RNA