title: Molecular characterization of human adenovirus type 8 (HAdV-8), including a novel genome type detected in Japan.
abstract: Human adenovirus type 8 (HAdV-8) is a common agent of severe epidemic keratoconjunctivitis  (EKC). Twenty-six strains were isolated from sporadic cases of EKC in the southern part of Japan  between 1998 and 1999 and were identified as HAdV-8 by the neutralization method using  type-specific antiserum against HAdV-8. A comparative analysis of different HAdV-8 genome types was  performed using various molecular methods. Restriction enzyme analyses of genomic DNA were  performed with BamHI, HindIII, PstI, SacI, SalI, and SmaI and identified 25 isolates as HAdV-8E and  1 isolate as HAdV-8J, a novel genome type. The genetic relatedness between HAdV-8J and the other  genome types was calculated by pairwise comigrating restriction fragments. The new genome type was  most genetically related to HAdV-8E. In a phylogram of both the hexon and fiber, HAdV-8J formed a  monophyletic cluster with other genome types of HAdV-8. Although HAdV-8J was identified from a  sporadic case of EKC, this strain may cause future outbreaks and thus warrants further monitoring.
authors: Department of Microbiology, BGC Trust Medical College, Chittagong, Bangladesh.
authors: Arun Kumar
authors: AK
authors: Adhikary
authors: Urmila
authors: U
authors: Banik
authors: Nobuhiko
authors: N
authors: Okabe
authors: Tsuguto
authors: T
authors: Fujimoto
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007564:Japan; D007637:Keratoconjunctivitis / Q000821:virology*; D058889:Molecular Typing; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D004279:DNA, Viral