title: Molecular epidemiology of Trichophyton tonsurans strains isolated in Japan between 2006 and 2010 and their susceptibility to oral antimycotics.
abstract: Trichophyton tonsurans has been isolated among judo practitioners, wrestlers, and  sumo wrestlers during an epidemic of tinea corporis and tinea capitis in Japan. A previous study  using restriction fragment length polymorphism (RFLP) analysis of the non-transcribed spacer (NTS)  region of the ribosomal RNA gene revealed that different sources for the causative fungus in  epidemics among judo practitioners and among wrestlers. Many different fungal strains have since  been isolated from practitioners of these sports. The present study evaluated fungal characteristics  of strains newly isolated between July 2006 and December 2010 using this molecular method. PCR-RFLP  analysis using MvaI and AvaI was performed on 263 strains, composed of 186 isolates from judo  practitioners, 32 from wrestlers, 30 from sumo wrestlers, 5 from other sports, 7 from family  members or friends of the sports practitioner patients, and 3 from sporadic (non-epidemic) cases.  Four molecular types, NTS I, II, III, and VII were detected. Of these, NTS I was the most  predominant, occurring in 243 of 263 strains (92.4%). All of the 30 strains isolated from sumo  wrestlers were classified as NTS I, suggesting that the epidemic among sumo wrestlers originated  from an earlier epidemic among judo practitioners. Thirteen strains were classified as NTS II;  all were related to wrestling and were isolated mainly from Chubu and Kansai areas in the central  part of Honshu island. NTS III was detected in 6 strains, and one strain classified as NTS VII was  isolated from a sporadic case of tinea capitis in a Peruvian immigrant. The minimum inhibitory  concentrations (MICs) of terbinafine, itraconazole, fluconazole, and griseofulvin on 10 strains  of NTS I and NTS II and 4 strains of NTS III were examined; there were no differences in MIC  between these molecular types.
authors: Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan.
authors: Kazushi
authors: K
authors: Anzawa
authors: Takashi
authors: T
authors: Mochizuki
authors: Akiko
authors: A
authors: Nishibu
authors: Hiroshi
authors: H
authors: Ishizaki
authors: Katsuhiko
authors: K
authors: Kamei
authors: Yoko
authors: Y
authors: Takahashi
authors: Machiko
authors: M
authors: Fujihiro
authors: Hidekazu
authors: H
authors: Shinoda
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D056352:Athletes; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014005:Tinea / Q000453:epidemiology* / Q000382:microbiology*; D014249:Trichophyton / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal