title: Fluorescence detection of single nucleotide polymorphisms using nucleic acid probe containing tricyclic base-linked acyclonucleoside.
abstract: A reliable and simple method for detecting nucleobase mutations is very important clinically because sequence variations in human DNA cause genetic diseases and genetically influenced traits. A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we developed a method for SNP detection using DNA probes that contained a fluorescent tricyclic base-linked acyclonucleoside N. The type of nucleobases involved in the SNP sites in an RNA target could be determined using four DNA probes containing N. Further, we found that the SNP in the RNA target could be detected by a visible color. Thus, this system would provide a novel and simple method for detecting SNPs in an RNA target.
authors: Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors: Mayumi
authors: M
authors: Hattori
authors: Tokimitsu
authors: T
authors: Ohki
authors: Emiko
authors: E
authors: Yanase
authors: Yoshihito
authors: Y
authors: Ueno
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics; D020137:Base Pair Mismatch; D065729:Cytochrome P-450 CYP2C9; D004247:DNA / Q000235:genetics; D005821:Genetic Techniques; D014644:Genetic Variation; D006801:Humans; D008956:Models, Chemical; D008957:Models, Genetic; D015341:Nucleic Acid Probes / Q000235:genetics*; D009696:Nucleic Acids; D009705:Nucleosides / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D015341:Nucleic Acid Probes; D009696:Nucleic Acids; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D004247:DNA; C450260:CYP2C9 protein, human; D065729:Cytochrome P-450 CYP2C9; D001189:Aryl Hydrocarbon Hydroxylases