title: Influence of sex and estrous cycle on synaptic responses of the medial vestibular nuclei in rats: role of circulating 17β-estradiol.
abstract: We investigated the possible influence of sex and estrous cycle on the synaptic responses of neurons in the medial vestibular nucleus (MVN) and their long-term modifications. In brain stem slices of male and female rats during proestrus (PE) and diestrus (DE), we evaluated the field potential evoked in the MVN by vestibular afferent stimulation. Here we find that in PE females the field potential had a lower threshold and higher amplitude than in DE females and in males and also that the stimulus-response curve was shifted to the left. Such difference is related to the level and cyclic fluctuation of circulating 17β-estradiol (E(2)). This is supported by the exogenous administration of E(2) in DE females and males, with low levels of circulating E(2) that enhanced the field potential amplitude to values close to those of PE females. Sex and estrous cycle also influence the MVN synaptic plasticity. This has been shown by investigating the effect of testosterone (T) on the induction of long-term effects, since T is the precursor for the neural synthesis of E(2) (estrogenic pathway), which is involved in the induction of fast long-term potentiation (LTP), or of 5α-dihydrotestosterone (DHT, androgenic pathway) which mediates slow LTP and long-term depression (LTD). We found that T mostly induced LTD in PE females and no effect in DE females, while it only provoked fast LTP in males. We suggest that high level of circulating E(2) may interfere with the conversion of T, by inhibiting the neural estrogenic pathway and facilitating the androgenic one. On the whole these results demonstrate an influence of circulating E(2) on vestibular synaptic transmission and plasticity that in some cases may contribute to the sex and menstrual cycle dependence of symptoms in human vestibular pathology.
authors: Dipartimento di Medicina Interna, Sezione di Fisiologia Umana, Università di Perugia, Via del Giochetto, I-06126 Perugia, Italy. sgrassi@unipg.it
authors: Silvarosa
authors: S
authors: Grassi
authors: Adele
authors: A
authors: Frondaroli
authors: Mariangela
authors: M
authors: Scarduzio
authors: Cristina V
authors: CV
authors: Dieni
authors: Gabriele
authors: G
authors: Brecchia
authors: Cristiano
authors: C
authors: Boiti
authors: Vito E
authors: VE
authors: Pettorossi
mesh_terms: D000704:Analysis of Variance; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism* / Q000494:pharmacology; D030762:Estrous Cycle / Q000187:drug effects / Q000502:physiology*; D005071:Evoked Potentials / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018408:Patch-Clamp Techniques; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013739:Testosterone / Q000494:pharmacology; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology; D014726:Vestibular Nuclei / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D000728:Androgens; D004967:Estrogens; D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; C062865:2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; D013739:Testosterone; D004958:Estradiol; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine