title: Simultaneous estimation of etoricoxib and thiocolchicoside by RP-HPLC method in combined dosage forms.
abstract: A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of etoricoxib and thiocolchicoside in combined tablet dosage form. Formulation containing etoricoxib and thiocolchicoside is used as analgesic. Chromatography was performed on a 250 mm x 4.6 mm, 5-microm particle size, BDS Hypersil C-18 column with trifluoroacetic acid buffer (pH 2.6) and acetonitrile (75:25, v/v) as a mobile phase. The detection of the combined dosage form was carried out at 220 nm and a flow rate employed was 1.5 mL/min. The retention times were 6.6 and 3.1 min for etoricoxib and thiocolchicoside, respectively. Linearity was obtained in the concentration range 20 to 160 ppm for etoricoxib and in the range 2 to 16 ppm for thiocolchicoside with a correlation coefficient of 0.9918 and 0.9994, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method.
authors: Department of Pharmacy, Barkatullah University, Bhopal M.P. India. sanjivkumar03@gmail.com
authors: Sanjiv
authors: S
authors: Kumar
authors: Amit
authors: A
authors: Joshi
authors: Rahul Singh
authors: RS
authors: Thakur
authors: Anupam K
authors: AK
authors: Pathak
authors: Kamal
authors: K
authors: Shah
mesh_terms: D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D056148:Chromatography, Reverse-Phase / Q000592:standards; D003078:Colchicine / Q000031:analogs & derivatives* / Q000032:analysis; D052246:Cyclooxygenase 2 Inhibitors / Q000032:analysis*; D004338:Drug Combinations; D004339:Drug Compounding; D000077613:Etoricoxib; D006863:Hydrogen-Ion Concentration; D011725:Pyridines / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013450:Sulfones / Q000032:analysis*; D013607:Tablets
chemical_list: D002021:Buffers; D052246:Cyclooxygenase 2 Inhibitors; D004338:Drug Combinations; D011725:Pyridines; D012997:Solvents; D013450:Sulfones; D013607:Tablets; D003078:Colchicine; C004280:thiocolchicoside; D000077613:Etoricoxib