title: Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from Streptomyces antibioticus in batch and repeated batch modes.
abstract: An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3) with various molecular chaperones. The purified recombinant OleD GT catalyzed glycosylation of various flavonoids: apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, 4-methylumbelliferone, naringenin, quercetin and resveratrol with UDP-glucose. 4.6 μg OleD GT was readily immobilized onto 1 mg hybrid nanoparticles of Fe(3)O(4)/silica/NiO on the basis of the affinity between His-tag and NiO nanoparticles with retention of 90% activity. In batch reaction, more than 90% naringenin (20 μM) was converted to its glycoside in 5 h. The immobilized OleD GT was efficiently reused for seven times whilst maintaining >60% of the residual activity in repeated glycosylation of naringenin.
authors: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors: Sung Hee
authors: SH
authors: Choi
authors: Mili
authors: M
authors: Ryu
authors: Yeo Joon
authors: YJ
authors: Yoon
authors: Dong-Myung
authors: DM
authors: Kim
authors: Eun Yeol
authors: EY
authors: Lee
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism*; D015870:Gene Expression; D006031:Glycosylation; D016695:Glycosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013303:Streptomyces antibioticus / Q000201:enzymology* / Q000235:genetics; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005419:Flavonoids; D011994:Recombinant Proteins; D016695:Glycosyltransferases; D014532:Uridine Diphosphate Glucose