title: Filamentous, mixed micelles of triblock copolymers enhance tumor localization of indocyanine green in a murine xenograft model.
abstract: Polymeric micelles formed by the self-assembly of amphiphilic block copolymers can be used to encapsulate hydrophobic drugs for tumor-delivery applications. Filamentous carriers with high aspect ratios offer potential advantages over spherical carriers, including prolonged circulation times. In this work, mixed micelles composed of poly(ethylene oxide)-poly[(R)-3-hydroxybutyrate]-poly(ethylene oxide) (PEO-PHB-PEO) and Pluronic F-127 (PF-127) were used to encapsulate a near-infrared fluorophore. The micelle formulations were assessed for tumor accumulation after tail vein injection to xenograft tumor-bearing mice by noninvasive optical imaging. The mixed micelle formulation that facilitated the highest tumor accumulation was shown by cryo-electron microscopy to be filamentous in structure compared to spherical structures of pure PF-127 micelles. In addition, increased dye loading efficiency and dye stability were attained in this mixed micelle formulation compared to pure PEO-PHB-PEO micelles. Therefore, the optimized PEO-PHB-PEO/PF-127 mixed micelle formulation offers advantages for cancer delivery over micelles formed from the individual copolymer components.
authors: Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.
authors: Tae Hee
authors: TH
authors: Kim
authors: Christopher W
authors: CW
authors: Mount
authors: Benjamin W
authors: BW
authors: Dulken
authors: Jenelyn
authors: J
authors: Ramos
authors: Caroline J
authors: CJ
authors: Fu
authors: Htet A
authors: HA
authors: Khant
authors: Wah
authors: W
authors: Chiu
authors: Wayne R
authors: WR
authors: Gombotz
authors: Suzie H
authors: SH
authors: Pun
mesh_terms: D000818:Animals; D055598:Chemical Phenomena; D003287:Contrast Media / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D004339:Drug Compounding; D004355:Drug Stability; D006801:Humans; D007208:Indocyanine Green / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008297:Male; D008546:Melanoma, Experimental / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D000091402:Prohibitins; D013047:Specific Pathogen-Free Organisms; D019265:Spectroscopy, Near-Infrared; D014018:Tissue Distribution; D051598:Whole Body Imaging; D000094122:Polyhydroxybutyrates
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D007208:Indocyanine Green; D008823:Micelles; D020442:Poloxamer; D011092:Polyethylene Glycols; D000091402:Prohibitins; C000717328:PHB protein, human; C512724:poly(ethylene oxide)-poly(3-hydroxybutyrate)-poly(ethylene oxide); D000094122:Polyhydroxybutyrates