title: Transgene traceability in transgenic mice: a bioanalytical approach for potential gene-doping analysis.
abstract: BACKGROUND
  The World Anti-Doping Agency fears the use of gene doping to enhance athletic performances. Thus, a bioanalytical approach based on end point PCR for detecting markers' of transgenesis traceability was developed.
  
  
  RESULTS
  A few sequences from two different vectors using an animal model were selected and traced in different tissues and at different times. In particular, enhanced green fluorescent protein gene and a construct-specific new marker were targeted in the analysis. To make the developed detection approach open to future routine doping analysis, matrices such as urine and tears as well blood were also tested.
  
  
  CONCLUSIONS
  This study will have impact in evaluating the vector transgenes traceability for the detection of a gene doping event by non-invasive sampling.
authors: Dipartimento di Biologia Evoluzionistica Leo Pardi, Università degli Studi di Firenze, Via Romana 17-19, Florence, Italy.
authors: Patrizia
authors: P
authors: Bogani
authors: Maria Michela
authors: MM
authors: Spiriti
authors: Stefano
authors: S
authors: Lazzarano
authors: Annarosa
authors: A
authors: Arcangeli
authors: Marcello
authors: M
authors: Buiatti
authors: Maria
authors: M
authors: Minunni
mesh_terms: D000818:Animals; D004300:Doping in Sports / Q000517:prevention & control*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018014:Gene Transfer Techniques; D018773:Genes, erbB-1; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D019076:Transgenes
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins