title: RNAi targeting CXCR4 inhibits tumor growth through inducing cell cycle arrest and apoptosis.
abstract: CXC chemokine receptor 4 (CXCR4) is involved in many human malignant tumors and plays an important role in tumor growth and metastasis. To explore the effects of CXCR4 expression on the malignant cells of oral squamous cell carcinoma (OSCC), Tca8113 and SCC-9 cell lines, as well as their xenograft models, of nude mice were used to detect cancer cell proliferation alteration. This study also examined the corresponding molecular mechanism after CXCR4 knockdown using a recombinant lentiviral vector expressing small interference RNA (siRNA) for CXCR4. RNA interference-mediated knockdown of CXCR4 in highly aggressive (Tca8113 and SCC-9) tumor cells significantly inhibited the proliferation of the two cell lines in vitro and in vivo. The expression levels of >1,500 genes involved in cell cycle, apoptosis, and multiple signaling pathways were also altered. These results provide new evidence of CXCR4 as a promising tumor gene therapeutic target.
authors: Department of Head and Neck Oncology Surgery, West China College of Stomatology, Sichuan University, Sichuan, People's Republic of China.
authors: Tao
authors: T
authors: Yu
authors: Yingying
authors: Y
authors: Wu
authors: Yi
authors: Y
authors: Huang
authors: Chaoran
authors: C
authors: Yan
authors: Ying
authors: Y
authors: Liu
authors: Zongsheng
authors: Z
authors: Wang
authors: Xiaoyi
authors: X
authors: Wang
authors: Yuming
authors: Y
authors: Wen
authors: Changmei
authors: C
authors: Wang
authors: Longjiang
authors: L
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016000:Cluster Analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors* / Q000235:genetics; D014161:Transduction, Genetic; D023041:Xenograft Model Antitumor Assays
chemical_list: D019394:Ki-67 Antigen; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019718:Receptors, CXCR4