title: Tailoring of biomimetic high-density lipoprotein nanostructures changes cholesterol binding and efflux.
abstract: Gold nanoparticles (Au NPs) were employed as templates to synthesize spherical, high-density lipoprotein (HDL) biomimics (HDL Au NPs) of different sizes and surface chemistries. The effect of size and surface chemistry on the cholesterol binding properties and the ability of the HDL Au NPs to efflux cholesterol from macrophage cells were measured. Results demonstrate that Au NPs may be utilized as templates to generate nanostructures with different physical characteristics that mimic natural HDL. Furthermore, the properties of the HDL Au NPs may be tailored to modulate the ability to bind cholesterol in solution and efflux cholesterol from macrophages. From the conjugates tested, the optimum size and surface chemistry for preparing functional Au NP-templated HDL biomimics were identified.
authors: Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
authors: Andrea J
authors: AJ
authors: Luthi
authors: Heng
authors: H
authors: Zhang
authors: Dongwoo
authors: D
authors: Kim
authors: David A
authors: DA
authors: Giljohann
authors: Chad A
authors: CA
authors: Mirkin
authors: C Shad
authors: CS
authors: Thaxton
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D004058:Diffusion; D006046:Gold / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D008075:Lipoproteins, HDL; D046911:Macromolecular Substances; D006046:Gold; D002784:Cholesterol