title: Culture of human corneal endothelial cells isolated from corneas with Fuchs endothelial corneal dystrophy.
abstract: The purpose of this study was to assess the feasibility of initiating primary cultures of corneal endothelial cells from patients suffering from Fuchs endothelial corneal dystrophy (FECD; MIM# 1036800). We also evaluated which conditions yielded the best results for culture. Twenty-nine patients undergoing Descemet stripping automated endothelial keratoplasty consented to the use of their excised Descemet's membrane for this study. Out of the 29 specimens, 18 successfully initiated a culture. Cell morphology varied between endothelial (rounded, slightly elongated cells, n = 12) and fibroblastic-like (thin and very elongated cells, n = 6). These differences in cell morphology were also observed with the normal human corneal endothelial cell cultures. The cultures that initially presented an endothelial morphology maintained their shape in subcultures. Clusterin expression was similar in FECD and normal endothelial cells. Transmission electron microscopy of FECD Descemet's membranes showed a high degree of various abnormalities generally found in this disease, such as a thickened Descemet's membrane, presence of a posterior banded layer, presence of a fibrillar layer and striated bodies of various sizes and periodicities. Patient's age was predictive of culture success, all younger FECD donors generating cultures of endothelial morphology. The absence of a fibrillar layer was also a factor associated with greater success. Culture success was not dependent on specimen size, specimen pigmentation, or patient's preoperative central corneal thickness. In conclusion, this paper shows for the first time that central Descemet's membranes of patients suffering from FECD possess proliferative endothelial cells that can be isolated and cultured without viral transduction, opening the way for new in vitro studies of this disease.
authors: Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération: LOEX-Centre de Recherche FRSQ du Centre Hospitalier affilié Universitaire de Québec, Québec, QC, Canada.
authors: Karine
authors: K
authors: Zaniolo
authors: Cristina
authors: C
authors: Bostan
authors: Olivier
authors: O
authors: Rochette Drouin
authors: Alexandre
authors: A
authors: Deschambeault
authors: Marie-Claude
authors: MC
authors: Perron
authors: Isabelle
authors: I
authors: Brunette
authors: Stéphanie
authors: S
authors: Proulx
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D051152:Clusterin / Q000378:metabolism; D003886:Descemet Membrane / Q000648:ultrastructure; D004728:Endothelium, Corneal / Q000378:metabolism / Q000473:pathology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005642:Fuchs' Endothelial Dystrophy / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; C495242:CLU protein, human; D051152:Clusterin; D007633:Keratins