title: Increased proportion of nitric oxide synthase immunoreactive neurons in rat ileal myenteric ganglia after severe acute pancreatitis.
abstract: BACKGROUND
  Severe acute pancreatitis (SAP) remains a potentially life-threatening disease. Gastrointestinal motility disturbance such as intestinal ileus is seen in every case. By now, the mechanisms of pancreatitis-induced ileus are largely unknown. The main purpose of the present study was to observe changes of nitric oxide synthase-immunoreactive (NOS-IR) neurons in ileal myenteric ganglia in SAP rats with gastrointestinal dysmotility, trying to explore underlying nervous mechanisms of pancreatitis-induced ileus.
  
  
  METHODS
  Twenty Sprague Dawley rats were randomly divided into sham operated group and SAP group. SAP was induced by retrograde cholangiopancreatic duct injection of 5% sodium taurocholate. Abdominal X-ray and intestinal transit were performed to detect the existence of paralytic ileus and intestinal dysmotility. Pathological damage of pancreas was evaluated. Double-immunolabeling was employed for the whole-mount preparations of ileal myenteric ganglia. The morphology of NOS-IR neurons were observed and the percentage of NOS-IR neurons was calculated based on the total Hu-immunoreactive neurons. Total RNA of ileum was extracted according to Trizol reagent protocol. Neuronal NOS (nNOS) mRNA expression was evaluated by RT-PCR.
  
  
  RESULTS
  The small intestinal transit index in the SAP group was significantly lower compared with the sham operated group (29.21±3.68% vs 52.48±6.76%, P<0.01). The percentage of NOS-IR neurons in ileal myenteric ganglia in the SAP group was significantly higher than that in the sham operated group (37.5±12.28% vs 26.32±16.15%, P<0.01). nNOS mRNA expression in ileum of SAP group was significantly higher than that in the sham operated group (1.02±0.10 vs 0.70±0.06, P<0.01).
  
  
  CONCLUSIONS
  The increased quantity of NOS-IR neurons in ileal myenteric ganglia and increased nNOS mRNA expression may suggest nNOS over expression as one of the nervous mechanisms of gastrointestinal dysmotility in SAP rat.
authors: Department of Gastroenterology and Hepatology, The Affiliated Hospital of Guilin Medical College, 15 Lequn Street, Guilin 541001, Guangxi Zuang Autonomous Region, People's Republic of China. Lin1955cn@163.com.
authors: Zhong
authors: Z
authors: Lin
authors: Ying
authors: Y
authors: Liu
authors: Qinghua
authors: Q
authors: Zheng
authors: Qinghua
authors: Q
authors: Hu
mesh_terms: D000818:Animals; D005769:Gastrointestinal Motility / Q000502:physiology*; D007082:Ileum / Q000201:enzymology / Q000294:innervation*; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D010195:Pancreatitis / Q000201:enzymology* / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D052248:Nitric Oxide Synthase Type I