title: Immunomodulatory effect of Withania somnifera supplementation diet in the giant freshwater prawn Macrobrachium rosenbergii (de Man) against Aeromonas hydrophila.
abstract: The effect of Withania somnifera extract supplementation diets on innate immune response in giant freshwater prawn Macrobrachium rosenbergii (de Man) against Aeromonas hydrophila was investigated. The bacterial clearance efficiency significantly increased in prawn fed with 0.1% and 1.0% doses of W. somnifera supplementation diet against pathogen from weeks 1-4 as compared to the control. The innate immune parameters such as, phenoloxidase activity, superoxide anion level, superoxide dismutase activity, nitrate, and nitrite concentrations were significantly enhanced in prawn fed with 0.1% and 1.0% doses of W. somnifera supplementation diet from weeks 1-4 against pathogen. The total hemocyte counts (THC) significantly increased in prawn fed with 0.1% and 1.0% doses diet from weeks 1-4 against pathogen as compared to the control. These results strongly suggested that administration of W. somnifera through supplementation diet positively enhances the innate immune system and enhanced survival rate in M. rosenbergii against A. hydrophila infection.
authors: Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea. rhari123@yahoo.com
authors: Ramasamy
authors: R
authors: Harikrishnan
authors: Chellam
authors: C
authors: Balasundaram
authors: Sundaram
authors: S
authors: Jawahar
authors: Moon-Soo
authors: MS
authors: Heo
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology*; D000818:Animals; D001772:Blood Cell Count; D019587:Dietary Supplements; D006434:Hemocytes / Q000737:chemistry; D007113:Immunity, Innate / Q000276:immunology*; D014442:Monophenol Monooxygenase / Q000032:analysis; D009566:Nitrates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D033541:Palaemonidae / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000032:analysis; D016019:Survival Analysis; D032341:Withania / Q000276:immunology
chemical_list: D009566:Nitrates; D009573:Nitrites; D013481:Superoxides; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase