title: Dimethylsulfoxide (DMSO) induces downregulation of heme oxygenase-1 (HO-1) in HL-60 cells: involvement of HO-1 in HL-60 cell differentiation.
abstract: Heme oxygenase-1 (HO-1), an inducible enzyme with broad tissue expression, is wel1-regulated in response to hematopoietic stress and preserves vascular homeostasis. We investigated the involvement of HO-1 in HL-60 cell differentiation. Dimethyl sulfoxide (DMSO) completely decreased HO-1 expression in a time-dependent manner, but clearly induced HL-60 cell differentiation, as evidenced by a marked increase in CD11b expression. Interestingly, zinc protoporphyrin (ZnPP), a strong inhibitor of HO-1, induced HL-60 cell differentiation. In contrast, treatment with cobalt protoporphyrin (CoPP), an activator of HO-1, decreased CD11b expression. Additionally, ZnPP downregulated HO-1 protein expression in HL-60 cells, whereas CoPP induced upregulation. These results suggest that HO-1 might have a negative function in DMSO-induced HL-60 cell differentiation. This study provides the first evidence that HO-1 plays an important role in DMSO-induced HL-60 cell differentiation.
authors: Department of Biochemistry, Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju, Korea.
authors: Eun-Mi
authors: EM
authors: Noh
authors: Dong-Hyu
authors: DH
authors: Cho
authors: Young-Rae
authors: YR
authors: Lee
authors: Young-Ju
authors: YJ
authors: Jeong
authors: Jong-Hyeon
authors: JH
authors: Kim
authors: Hee Suk
authors: HS
authors: Chae
authors: Jinny
authors: J
authors: Park
authors: Won-Seok
authors: WS
authors: Jung
authors: Sung-Joo
authors: SJ
authors: Park
authors: Jong-Suk
authors: JS
authors: Kim
mesh_terms: D039481:CD11b Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects*; D018922:HL-60 Cells; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D011524:Protoporphyrins / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D039481:CD11b Antigen; D011524:Protoporphyrins; C017803:zinc protoporphyrin; C007095:cobaltiprotoporphyrin; D051547:Heme Oxygenase-1; D004121:Dimethyl Sulfoxide