title: In vitro evaluation of cytotoxicity of denture adhesives.
abstract: OBJECTIVE
  The aim of the study is to assess and compare the cytotoxicity of commercially available four denture adhesives ex-vivo.
  
  
  METHODS
  Four commercially available denture adhesives namely Metrodent powder, Fixon powder, Dentiro powder and Fixon cream were selected. Normal saline was used in control group. To evaluate the cytotoxicity of denture adhesives, macrophages were isolated from peritoneal cavity of Swiss albino mice and cell integrity/cell viability method was done by using trypan blue dye.
  
  
  RESULTS
  Viable cells were counted and subjected to statistical analysis. ANOVA, F and 't' test were performed, which showed statistically significant values (P < 0.001). The mean percentage of viable cells was highest in the control group (95%) and lowest in Fixon powder (55.66%), with Dentiro powder the mean percentage of viable cells was 63.66%, with Metrodent powder 67.6% while with Fixon cream it was 69.33%.
  
  
  CONCLUSIONS
  All tested denture adhesives showed varied degree of cytotoxicity that is statistically significant. The degree of toxicity was more in Fixon powder followed by Dentiro powder and Metrodent powder with least in Fixon cream.
authors: Department of Prosthodontics, Kothiwal Dental College and Research Centre, Moradabad, Uttar Pradesh, India. dr.samarthagarwal@yahoo.co.in
authors: Samarth Kumar
authors: SK
authors: Agarwal
authors: G
authors: G
authors: Praveen
authors: Saurabh
authors: S
authors: Gupta
authors: Renu
authors: R
authors: Tandon
authors: Sameer
authors: S
authors: Gupta
mesh_terms: D000269:Adhesives / Q000633:toxicity*; D000818:Animals; D002452:Cell Count; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D003781:Denture Retention; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D014343:Trypan Blue
chemical_list: D000269:Adhesives; D004396:Coloring Agents; D014343:Trypan Blue