title: [Generation of mouse parthenogenetic embryonic stem cells and preliminary study of the differentiation ability to motor neurons].
abstract: In this study, we generated embryonic stem cells from parthenogenetic embryos (PESCs), and induced them to differentiate to motor neurons, which could be an alternative source of histocompatible cells for replacement of therapy and theoretical foundation for studying the relationship of genome imprint and neural differentiation. The parthenogenetic activation rate of B6D2F1 mouse oocytes was 93.26%. We established eight parthenogenetic embryonic stem cell lines and the establishment rate from parthenogenetic embryos was 23.53%. The pluripotency marker Oct4, the cell surface marker SSEA-1, and alkaline phosphatase exhibited in PESCs. Karyotype analysis showed normal 40 chromosomes when examined at passages 10 and 30, which was in accordance with their oocyte origins. Three germinal layers were differentiated in vivo and in vitro. Mouse PESCs, which were treated by tretinoin and sonic hedgehog with extracellular matrix, could generate motor neurons that expressed the specific markers such as HB9 and Olig2.
authors: Department of Histology and Embryology, Harbin Medical University, Harbin, China. geraldon1122@yahoo.com.cn
authors: Zhen-Dong
authors: ZD
authors: Wang
authors: Yuan
authors: Y
authors: Xue
authors: Zhi-Yan
authors: ZY
authors: Shan
authors: Zhong
authors: Z
authors: Zheng
authors: Xue
authors: X
authors: Li
authors: Yan-Shuang
authors: YS
authors: Wu
authors: Rui-Zhen
authors: RZ
authors: Sun
authors: Jian
authors: J
authors: Shi
authors: Ming-Jie
authors: MJ
authors: Li
authors: Zhong-Hua
authors: ZH
authors: Liu
authors: Lei
authors: L
authors: Lei
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009046:Motor Neurons / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D010312:Parthenogenesis
chemical_list: D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse