title: Improvement of the biocatalytic properties of one phenylacetone monooxygenase mutant in hydrophilic organic solvents.
abstract: The presence of different hydrophilic organic solvents or a water soluble polymer such as PEG 4000 led to an enhancement in the enzymatic activity of the M446G mutant of phenylacetone monooxygenase when it is employed in enantioselective sulfoxidations and Baeyer-Villiger reactions. By solvent engineering new substrates were found to be effectively converted by this Baeyer-Villiger monooxygenase. The use of 5% methanol together with the weak anion exchange resin Lewatit MP62 also allows the dynamic kinetic resolution of a set of racemic benzylketones. By this approach (S)-benzylesters could be obtained with high yields and optical purities.
authors: Departamento de Química Orgánica e Inorgánica, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, c/Julián Clavería 8, 33006 Oviedo, Spain. gonzalogonzalo@uniovi.es
authors: Gonzalo
authors: G
authors: de Gonzalo
authors: Cristina
authors: C
authors: Rodríguez
authors: Ana
authors: A
authors: Rioz-Martínez
authors: Vicente
authors: V
authors: Gotor
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives* / Q000378:metabolism; D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D004795:Enzyme Stability; D057927:Hydrophobic and Hydrophilic Interactions; D007475:Ion Exchange Resins; D007536:Isomerism; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012997:Solvents; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D007475:Ion Exchange Resins; D007659:Ketones; D050505:Mutant Proteins; D011994:Recombinant Proteins; D012997:Solvents; D000096:Acetone; D006899:Mixed Function Oxygenases; C008863:1-phenyl-2-propanone