title: N-acyl-homoserine lactones from Enterobacter sakazakii (Cronobacter spp.) and their degradation by Bacillus cereus enzymes.
abstract: A chemical study of acyl-homoserine lactones (acyl-HSLs) produced by Enterobacter sakazakii resulted in the identification of three molecules: (S)-N-heptanoyl-HSL, (S)-N-dodecanoyl-HSL and (S)-N-tetradecanoyl-HSL. Mixed cultures of E. sakazakii and Bacillus cereus depleted E. sakazakii acyl-HSLs, suggesting acyl-HSL degradation by B. cereus hydrolases (hydrolysis of the lactone or amide moiety). The expression of B. cereus acyl-HSL lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransformation of (S)-N-dodecanoyl-HSL into (S)-N-dodecanoyl-homoserine, dodecanoic acid and homoserine in the presence of B. cereus whole cells, using electrospray-mass spectrometry (ESI-MS).
authors: Chemistry Institute, University of Campinas, Campinas, SP, Brazil.
authors: Francisca Diana da Silva
authors: FD
authors: Araújo
authors: Luciana Maria Ramires
authors: LM
authors: Esper
authors: Arnaldo Yoshiteru
authors: AY
authors: Kuaye
authors: Marcelo Palma
authors: MP
authors: Sircili
authors: Anita Jocelyne
authors: AJ
authors: Marsaioli
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism; D001409:Bacillus cereus / Q000201:enzymology*; D018920:Coculture Techniques; D044083:Cronobacter sakazakii / Q000737:chemistry / Q000378:metabolism*; D006714:Homoserine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D054742:Acyl-Butyrolactones; C516096:N-dodecanoyl-DL-homoserine lactone; C503769:N-heptanoyl-homoserine lactone; D006714:Homoserine; D000581:Amidohydrolases; D015107:4-Butyrolactone