title: Spirostanol saponins derivated from the seeds of Trigonella foenum-graecum by β-glucosidase hydrolysis and their inhibitory effects on rat platelet aggregation.
abstract: Nine spirostanol saponins (1-9) and seven mixtures of 25 R and 25 S spirostanol saponin isomers (10-16) were obtained from the seeds of Trigonella foenum-graecum after enzymatic hydrolysis of the furostanol saponin fraction by β-glucosidase. Their structures were determined by NMR and MS spectroscopy. Among them, 1- 4, 6, 8, and 9 were new compounds and five, 11B, 12A, 13B, 14A, and 14B, were new structures observed from seven mixtures. In addition, the inhibitory effects of all saponins on rat platelet aggregation were evaluated.
authors: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, PR China.
authors: Xu
authors: X
authors: Pang
authors: Yue
authors: Y
authors: Cong
authors: He-Shui
authors: HS
authors: Yu
authors: Li-Ping
authors: LP
authors: Kang
authors: Bing
authors: B
authors: Feng
authors: Bing-Xing
authors: BX
authors: Han
authors: Yang
authors: Y
authors: Zhao
authors: Cheng-Qi
authors: CQ
authors: Xiong
authors: Da-Wei
authors: DW
authors: Tan
authors: Wei
authors: W
authors: Song
authors: Bin
authors: B
authors: Liu
authors: Yu-Wen
authors: YW
authors: Cong
authors: Bai-Ping
authors: BP
authors: Ma
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006868:Hydrolysis; D008297:Male; D015394:Molecular Structure; D010936:Plant Extracts / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029910:Trigonella / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012503:Saponins; D013150:Spirostans; D001617:beta-Glucosidase