title: In vitro model of stromal and epithelial immortalized endometriotic cells.
abstract: Endometriosis is a relatively common chronic gynecologic disorder that usually presents with chronic pelvic pain or infertility. It results from implantation of endometrial tissue outside the uterine cavity. Despite its frequency and its impact on quality of life, the understanding of pathogenesis of endometriosis remains incomplete and its treatment remains controversial. In this work, we established a suitable in vitro model system of immortalized human endometriotic cell line taking advantage of the human telomerase reverse transcriptase. The results demonstrate that these cells retain the natural characteristics of endometrial cells in term of phenotype and of functional expression of estrogen and progesterone receptors, without chromosomal abnormalities. In conclusion, these cells are potentially useful as an experimental model to investigate endometriosis biology.
authors: Department of Biochemistry and Biophysics, Second University of Naples, Naples, Italy.
authors: Mariarosaria
authors: M
authors: Boccellino
authors: Lucio
authors: L
authors: Quagliuolo
authors: Annarita
authors: A
authors: Verde
authors: Raffaele
authors: R
authors: La Porta
authors: Stefania
authors: S
authors: Crispi
authors: Maria Teresa
authors: MT
authors: Piccolo
authors: Antonio
authors: A
authors: Vitiello
authors: Alfonso
authors: A
authors: Baldi
authors: Pietro Giulio
authors: PG
authors: Signorile
mesh_terms: D001483:Base Sequence; D002461:Cell Line, Transformed; D017931:DNA Primers; D004715:Endometriosis / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007621:Karyotyping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000473:pathology*
chemical_list: D017931:DNA Primers