title: HPLC method for identification and quantification of benzimidazole derivatives in antiparasitic drugs.
abstract: The subject of the study was to develop a versatile HPLC system for identification and determination of four benzimidazole derivatives in the antiparasitic drugs. The tests covered: Zentel, Panacur, Vermox tablets and Systamex suspension. A satisfactory separation was obtained using the Nucleosil C8 column in the gradient system composed of mobile phase A: 85% orthophosphoric acid / water / acetonitrile in 0.05:75:25, v/v/v ratio, and mobile phase B: 85% orthophosphoric acid / water / acetonitrile in 0.05:50:50, v/v/v ratio. Both phases were adjusted to pH = 4.5 with 15% sodium hydroxide solution. A detection at 288 nm for oxfendazole and 254 nm for albendazole, fenbendazole and mebendazole was applied. The correlation coefficients in the range 0,9997 - 0,9999 proved that the calibration curves were linear. The method was validated in terms of selectivity, accuracy and precision.
authors: Department of Basic and Applied Pharmacy, National Medicines Institute, 30/34 Chełmska St., 00-725 Warszawa, Poland. akulik@il.waw.pl
authors: Anna
authors: A
authors: Kulik
authors: Wanda
authors: W
authors: Białecka
authors: Marzena
authors: M
authors: Podolska
authors: Barbara
authors: B
authors: Kwiatkowska-Puchniarz
authors: Aleksander
authors: A
authors: Mazurek
mesh_terms: D015766:Albendazole / Q000032:analysis; D000978:Antiparkinson Agents / Q000032:analysis*; D001562:Benzimidazoles / Q000032:analysis*; D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D005273:Fenbendazole / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008463:Mebendazole / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D000978:Antiparkinson Agents; D001562:Benzimidazoles; D002021:Buffers; D012997:Solvents; D005273:Fenbendazole; D008463:Mebendazole; D015766:Albendazole; C011030:oxfendazole