title: Determination of L-phenylalanine on-line based on molecularly imprinted polymeric microspheres and flow injection chemiluminescence.
abstract: A novel molecular imprinting-chemiluminescence (MIP-CL) sensor for the determination of L-phenylalanine (Phe) using molecularly imprinted polymer (MIP) as recognition element is reported. The Phe-MIP was synthesized using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile (AIBN) as initiator and the polymers' properties were characterized. Then the synthesized MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of Phe in the range 1.3 × 10(-6) to 5.44 × 10(-4) mol/L with a detection limit of 6.23 × 10(-7) mol/L (3σ), which is lower than that of conventional methods. The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of Phe in samples.
authors: School of Chemistry and Chemical Engineering, University of Jinan, No. 106 Jiwei Road, Jinan 250022, China.
authors: Huamin
authors: H
authors: Qiu
authors: Yulei
authors: Y
authors: Xi
authors: Fuguang
authors: F
authors: Lu
authors: Lulu
authors: L
authors: Fan
authors: Chuannan
authors: C
authors: Luo
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D017022:Flow Injection Analysis; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D009862:Online Systems; D010649:Phenylalanine / Q000032:analysis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D059027:Recycling; D012044:Regression Analysis; D013055:Spectrophotometry, Infrared
chemical_list: D011108:Polymers; D010649:Phenylalanine