title: Relation between the frequency of CD34⁺ bone marrow derived circulating progenitor cells and the number of diseased coronary arteries in patients with myocardial ischemia and diabetes.
abstract: BACKGROUND
  Bone marrow-derived circulating progenitor cells (BM-CPCs) in patients with coronary heart disease are impaired with respect to number and mobilization. However, it is unknown whether the mobilization of BM-CPCs depends on the number of diseased coronary arteries. Therefore, in our study, we analysed the correlation between the diseased coronary arteries and the frequency of CD34/45+ BM-CPCs in peripheral blood (PB) in patients with ischemic heart disease (IHD).
  
  
  METHODS
  The frequency of CD34/45+ BM-CPCs was measured by flow cytometry in 120 patients with coronary 1 vessel (IHD1, n = 40), coronary 2 vessel (IHD2, n = 40), coronary 3 vessel disease (IHD3, n = 40) and in a control group of healthy subjects (n = 40). There was no significant difference of the total number of cardiovascular risk factors between IHD groups, beside diabetes mellitus (DM), which was significantly higher in IHD3 group compared to IHD2 and IHD1 groups.
  
  
  RESULTS
  The frequency of CD34/45+ BM-CPCs was significantly reduced in patients with IHD compared to the control group (CD34/45+; p < 0.001). The frequency of BM-CPCs was impaired in patients with IHD3 compared to IHD1 (CD34/45+; p < 0.001) and to IHD2 (CD34/45+; p = 0.001). But there was no significant difference in frequency of BM-CPCs between the patients with IHD2 and IHD1 (CD34/45+; p = 0.28). In a subgroup we observed a significant negative correlation between levels of hemoglobin AIc (HbAIc) and the frequency of BM-CPCs (CD34/45+; p < 0.001, r = -0.8).
  
  
  CONCLUSIONS
  The frequency of CD34/45+ BM-CPCs in PB is impaired in patients with IHD. This impairment may augment with an increased number of diseased coronary arteries. Moreover, the frequency of CD34/45+ BM-CPCs in ischemic tissue is further impaired by diabetes in patients with IHD.
authors: Department of Internal Medicine, Division of Cardiology, University Hospital Rostock, Germany.
authors: Ilkay
authors: I
authors: Bozdag-Turan
authors: R Goekmen
authors: RG
authors: Turan
authors: C Hakan
authors: CH
authors: Turan
authors: Sophie
authors: S
authors: Ludovicy
authors: Ibrahim
authors: I
authors: Akin
authors: Stephan
authors: S
authors: Kische
authors: Nicole S
authors: NS
authors: Arsoy
authors: Henrik
authors: H
authors: Schneider
authors: Jasmin
authors: J
authors: Ortak
authors: Tim
authors: T
authors: Rehders
authors: Tina
authors: T
authors: Hermann
authors: Liliya
authors: L
authors: Paranskaya
authors: Peter
authors: P
authors: Kohlschein
authors: Manuela
authors: M
authors: Bastian
authors: A Tulga
authors: AT
authors: Ulus
authors: Kurtulus
authors: K
authors: Sahin
authors: Hueseyin
authors: H
authors: Ince
authors: Christoph A
authors: CA
authors: Nienaber
mesh_terms: D000368:Aged; D018952:Antigens, CD34 / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001854:Bone Marrow Cells / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D002465:Cell Movement; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D003920:Diabetes Mellitus / Q000097:blood / Q000276:immunology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005858:Germany; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology*; D012720:Severity of Illness Index; D013234:Stem Cells / Q000276:immunology / Q000473:pathology*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D006442:Glycated Hemoglobin; D017493:Leukocyte Common Antigens; C517652:hemoglobin A1c protein, human; C516769:PTPRC protein, human