title: Effect of plumbagin free alcohol extract of Plumbago zeylanica Linn. root on reproductive system of female Wistar rats.
abstract: OBJECTIVE
  To assess the effect of plumbagin-free alcohol extract (PFAE) of Plumbago zeylanica Linn. (Plumbaginaceae) (P. zeylanica) root, on female reproductive system and fertility of adult female wistar rats.
  
  
  METHODS
  After the oral acute toxicity study, the PFAE was administered at two dose levels to perform the estrous cycle study, anti-implantation and abortifacient activity and hormonal analysis. However, the estrogenic/antiestrogenic activity was evaluated at only one most effective dose.
  
  
  RESULTS
  LD(50) cut-off was 5,000 mg/kg body weight. The extract exhibited significant anti-implantation and abortifacient activity at the tested dose levels (300 and 500 mg/kg, p.o.) (P<0.01). The extract dose-dependently decreased the levels of serum progesterone, follicle stimulating hormone and luteinizing hormone, while a dose-dependent increase was observed in the concentration of serum prolactin. The extract did not show any significant changes in structure and function of uterus when given alone, but when given along with ethinyl estradiol, it exhibited significant antiestrogenic activity in immature overiectomized female rats(P<0.001). Biochemical parameters in the serum/blood and haematological parameters did not show appreciable changes throughout and after the course of investigation. However, all the altered parameters returned to normalcy within 30 days following withdrawal of treatment.
  
  
  CONCLUSIONS
  All findings suggest that the antifertility activity of extract could possibly be through the changes in the implantation site, altered hormonal levels, prolonged estrous cycle and anti-estrogenic activity. Hence, the extract possesses reversible antifertility activity without adverse toxicity in female rats.
authors: School of Pharmacy, Chouksey Engineering College, Bilaspur, India. sand8483@yahoo.com
authors: Gupta
authors: G
authors: Sandeep
authors: Ahirwar
authors: A
authors: Dheeraj
authors: Neeraj Kumar
authors: NK
authors: Sharma
authors: Deenanath
authors: D
authors: Jhade
authors: Ahirwar
authors: A
authors: Bharti
mesh_terms: D000019:Abortifacient Agents / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D020847:Estrogen Receptor Modulators / Q000494:pharmacology*; D030762:Estrous Cycle / Q000187:drug effects*; D005260:Female; D005298:Fertility / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D029606:Plumbaginaceae; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000187:drug effects*
chemical_list: D000019:Abortifacient Agents; D020847:Estrogen Receptor Modulators; D010936:Plant Extracts; D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone