title: Efficient siRNA delivery using novel siRNA-loaded Bubble liposomes and ultrasound.
abstract: Recently, we developed novel polyethyleneglycol (PEG)-modified liposomes (Bubble liposomes; BLs) entrapping an ultrasound (US) imaging gas and reported that the combination of BLs and US was useful for the delivery of siRNA directly into the cytoplasm. However, the results were obtained using a mixture of BLs and naked siRNA. With systemic injections, it is important to control the biodistribution of both BLs and siRNA. In addition, the delivery of siRNA is affected by nuclease degradation after intravenous administration. In this study, we prepared novel siRNA-loaded BLs (si-BLs) using a cationic lipid, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). We demonstrated that siRNA could be loaded onto BLs containing DOTAP and that siRNA-loaded BLs were stable in serum. A specific gene-silencing effect was also achieved by transfection with si-BLs. Thus, the combination of si-BLs with US exposure can be used for delivery of siRNA to a specific tissue via systemic injection.
authors: Department of Drug Delivery and Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
authors: Yoko
authors: Y
authors: Endo-Takahashi
authors: Yoichi
authors: Y
authors: Negishi
authors: Yasuharu
authors: Y
authors: Kato
authors: Ryo
authors: R
authors: Suzuki
authors: Kazuo
authors: K
authors: Maruyama
authors: Yukihiko
authors: Y
authors: Aramaki
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism*; D007267:Injections; D008081:Liposomes; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D045423:Microbubbles; D011092:Polyethylene Glycols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism*; D034622:RNA Interference; D020871:RNA Stability; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics
chemical_list: D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane