title: Effectively and efficiently dissecting the infection of influenza virus by quantum-dot-based single-particle tracking.
abstract: Exploring the virus infection mechanisms is significant for defending against virus infection and providing a basis for studying endocytosis mechanisms. Single-particle tracking technique is a powerful tool to monitor virus infection in real time for obtaining dynamic information. In this study, we reported a quantum-dot-based single-particle tracking technique to efficiently and globally research the virus infection behaviors in individual cells. It was observed that many influenza viruses were moving rapidly, converging to the microtubule organizing center (MTOC), interacting with acidic endosomes, and finally entering the target endosomes for genome release, which provides a vivid portrayal of the five-stage virus infection process. This report settles a long-pending question of how viruses move and interact with acidic endosomes before genome release in the perinuclear region and also finds that influenza virus infection is likely to be a "MTOC rescue" model for genome release. The systemic technique developed in this report is expected to be widely used for studying the mechanisms of virus infection and uncovering the secrets of endocytosis.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine (MOE 985 Innovative Platform), Wuhan University, Wuhan 430072, People's Republic of China.
authors: Shu-Lin
authors: SL
authors: Liu
authors: Zhi-Ling
authors: ZL
authors: Zhang
authors: Zhi-Quan
authors: ZQ
authors: Tian
authors: Hai-Su
authors: HS
authors: Zhao
authors: Haibin
authors: H
authors: Liu
authors: En-Ze
authors: EZ
authors: Sun
authors: Geng Fu
authors: GF
authors: Xiao
authors: Wanpo
authors: W
authors: Zhang
authors: Han-Zhong
authors: HZ
authors: Wang
authors: Dai-Wen
authors: DW
authors: Pang
mesh_terms: D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D053127:Influenza A Virus, H9N2 Subtype / Q000648:ultrastructure*; D007251:Influenza, Human / Q000473:pathology* / Q000821:virology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D045663:Quantum Dots; D014771:Virion / Q000648:ultrastructure