title: The ferulic acid esterases of Chrysosporium lucknowense C1: purification, characterization and their potential application in biorefinery.
abstract: Three ferulic acid esterases from the filamentous fungus Chrysosporium lucknowense C1 were purified and characterized. The enzymes were most active at neutral pH and temperatures up to 45 °C. All enzymes released ferulic acid and p-coumaric acid from a soluble corn fibre fraction. Ferulic acid esterases FaeA1 and FaeA2 could also release complex dehydrodiferulic acids and dehydrotriferulic acids from corn fibre oligomers, but released only 20% of all ferulic acid present in sugar beet pectin oligomers. Ferulic acid esterase FaeB2 released almost no complex ferulic acid oligomers from corn fibre oligomers, but 60% of all ferulic acid from sugar beet pectin oligomers. The ferulic acid esterases were classified based on both, sequence similarity and their activities toward synthetic substrates. The type A ferulic acid esterases FaeA1 and FaeA2 are the first members of the phylogenetic subfamily 5 to be biochemically characterized. Type B ferulic acid esterase FaeB2 is a member of subfamily 6.
authors: Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
authors: S
authors: S
authors: Kühnel
authors: L
authors: L
authors: Pouvreau
authors: M M
authors: MM
authors: Appeldoorn
authors: S W A
authors: SW
authors: Hinz
authors: H A
authors: HA
authors: Schols
authors: H
authors: H
authors: Gruppen
mesh_terms: D056804:Biofuels; D018533:Biomass; D002265:Carboxylic Ester Hydrolases / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002912:Chrysosporium / Q000201:enzymology* / Q000235:genetics; D003373:Coumaric Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D010368:Pectins / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism
chemical_list: D056804:Biofuels; D003373:Coumaric Acids; D005656:Fungal Proteins; D014990:Xylans; D010368:Pectins; C085118:arabinoxylan; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase