title: Gene expression changes in the pituitary gland of rats exposed to electromagnetic pulses.
abstract: OBJECTIVE
  We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).
  
  
  METHODS
  The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis. We then validated and further investigated the reduced expression of two tumorigenesis-related genes, Pten, and Jund, by assessing their mRNA and protein expression by quantitative real-time-PCR, western blotting, and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.
  
  
  RESULTS
  EMP exposure induced genome-wide gene expression changes in the rat pituitary gland. There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.
  
  
  CONCLUSIONS
  EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland. These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
authors: Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China.
authors: YuHong
authors: Y
authors: Qi
authors: Jun
authors: J
authors: Liang
authors: YanPing
authors: Y
authors: Hui
authors: GuiRong
authors: G
authors: Ding
authors: JunYe
authors: J
authors: Liu
authors: XiaoMing
authors: X
authors: Su
authors: GuoZhen
authors: G
authors: Guo
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D015536:Down-Regulation; D055590:Electromagnetic Phenomena; D005260:Female; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C549531:JunD protein, rat; D016755:Proto-Oncogene Proteins c-jun; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat