title: Autofluorescence spectroscopy for evaluating dysplasia in colorectal tissues.
abstract: The aim of this study was to assess the applicability of autofluorescence (AF) spectroscopy as a method for the diagonosis of normal, benign and malignant of dysplasia in colorectal tissues experimentally. By improvement of optical design in laser pulse generator, wavelength-adjustable output was acquired and the optimal wavelength was defined as 380 nm. With 380-nm pulsed laser excitation, AF spectra of normal, benign and malignant colorectal tissues were recorded in the spectra region from 460-570 nm in vitro. The spectral analysis for discrimination among the different types of tissues was carried out using principal component analysis (PCA)-based Neural networks algorithm. The performance of analysis was pretty good with sensitivity, specificity and accuracy found to be 100%,90% and 96.7%, respectively. The AF spectroscopy may serve as an excellent tool for the evaluation of dysplasia in colorectal clinical diagnosis.
authors: Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
authors: Xiang-jian
authors: XJ
authors: Luo
authors: Bo
authors: B
authors: Zhang
authors: Jian-guo
authors: JG
authors: Li
authors: Xiang-an
authors: XA
authors: Luo
authors: Li-fang
authors: LF
authors: Yang
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000236:Adenoma / Q000473:pathology; D018253:Adenoma, Villous / Q000473:pathology; D000465:Algorithms; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D003111:Colonic Polyps / Q000473:pathology; D015179:Colorectal Neoplasms / Q000473:pathology*; D003937:Diagnosis, Differential; D006801:Humans; D006965:Hyperplasia; D007413:Intestinal Mucosa / Q000473:pathology; D053844:Lasers, Solid-State; D008223:Lymphoma / Q000473:pathology; D009361:Neoplasm Invasiveness; D016571:Neural Networks, Computer; D011230:Precancerous Conditions / Q000473:pathology*; D025341:Principal Component Analysis; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*