title: Population-based modeling of the progression of apoptosis in mammalian cell culture.
abstract: The production of biopharmaceuticals from mammalian cell culture is hindered by apoptosis, which is the primary cause of cell death in these cultures. As a tool for optimization of culture yield, this study presents a population-based model describing the progression of apoptosis in a monoclonal antibody (mAb)-producing Chinese hamster ovary (CHO) cell culture. Because mAb production does not cease when apoptosis begins, the model was designed to incorporate subpopulations at various stages in the progression of apoptosis. The model was validated against intracellular measurements of caspase activity as well as cell density, nutrient levels, and toxic metabolites. Since the specific details of apoptotic mechanisms have not been elucidated in this cell line, we employed a model comparison analysis that suggests the most plausible pathways of activation.
authors: Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada.
authors: Mukesh
authors: M
authors: Meshram
authors: Saeideh
authors: S
authors: Naderi
authors: Brendan
authors: B
authors: McConkey
authors: Hector
authors: H
authors: Budman
authors: Jeno
authors: J
authors: Scharer
authors: Brian
authors: B
authors: Ingalls
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D017209:Apoptosis; D001709:Biotechnology / Q000379:methods; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D008954:Models, Biological; D015233:Models, Statistical; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins