title: A retinoic acid responsive Hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field.
abstract: A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.
authors: Molecular and Cellular Animal Embryology group, Life Sciences Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
authors: Nata Y S-G
authors: NY
authors: Diman
authors: Sophie
authors: S
authors: Remacle
authors: Nicolas
authors: N
authors: Bertrand
authors: Jacques J
authors: JJ
authors: Picard
authors: Stéphane
authors: S
authors: Zaffran
authors: René
authors: R
authors: Rezsohazy
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000172:deficiency; D000818:Animals; D004707:Endoderm / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006321:Heart / Q000196:embryology* / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D008297:Male; D051379:Mice; D009432:Neural Crest / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010614:Pharynx / Q000196:embryology*; D015398:Signal Transduction / Q000187:drug effects; D019076:Transgenes / Q000235:genetics; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D018398:Homeodomain Proteins; C080331:Hoxa3 protein, mouse; D014212:Tretinoin; D000445:Aldehyde Oxidoreductases; C470579:RALDH2 protein, mouse