title: HIV-1 Tat induces biochemical changes in the serum of mice.
abstract: The HIV-1 Tat protein is released by infected cells and has numerous biological activities which might contribute either to the impairment of the immune response or to viral dissemination and pathogenesis. To date, the effects of Tat protein on metabolites remain unclear. In this study, a metabolomic study on serum of HIV-1 Tat-induced ICR mice was performed to research the pathologic mechanism of Tat protein by using gas chromatography coupled to mass spectrometry (GC/MS). Clear separations among the HIV-1 Tat-induced mice and the inaTat-induced or control mice were observed by principal component analysis and partial least-squares discriminant analysis based on the GC/MS spectral data. Additionally, 16 significantly changed metabolites in HIV-1 Tat-induced mice were identified that are involved in multiple perturbed metabolic pathways, which contributed to the elucidation of the complex pathogenic mechanism of Tat protein and may shed new lights on future improvement of HIV-1 therapy.
authors: School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors: Wenting
authors: W
authors: Liao
authors: Guangguo
authors: G
authors: Tan
authors: Zhenyu
authors: Z
authors: Zhu
authors: Qiuli
authors: Q
authors: Chen
authors: Ziyang
authors: Z
authors: Lou
authors: Xin
authors: X
authors: Dong
authors: Wei
authors: W
authors: Zhang
authors: Wei
authors: W
authors: Pan
authors: Yifeng
authors: Y
authors: Chai
mesh_terms: D000818:Animals; D016002:Discriminant Analysis; D008401:Gas Chromatography-Mass Spectrometry; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D057809:HEK293 Cells; D015658:HIV Infections / Q000097:blood* / Q000821:virology*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009154:Mutation; D025341:Principal Component Analysis; D011994:Recombinant Proteins; D015533:Transcriptional Activation; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D014364:Tryptophan