title: A proteomic analysis of the functional effects of fatty acids in NIH 3T3 fibroblasts.
abstract: Previous studies have demonstrated that long chain fatty acids influence fibroblast function at sub-lethal concentrations. This study is the first to assess the effects of oleic, linoleic or palmitic acids on protein expression of fibroblasts, as determined by standard proteomic techniques. The fatty acids were not cytotoxic at the concentration used in this work as assessed by membrane integrity, DNA fragmentation and the MTT assay but significantly increased cell proliferation. Subsequently, a proteomic analysis was performed using two dimensional difference gel electrophoresis (2D-DIGE) and MS based identification. Cells treated with 50 μM oleic, linoleic or palmitic acid for 24 h were associated with 24, 22, 16 spots differentially expressed, respectively. Among the identified proteins, α-enolase and far upstream element binding protein 1 (FBP-1) are of importance due to their function in fibroblast-associated diseases. However, modulation of α-enolase and FBP-1 expression by fatty acids was not validated by the Western blot technique.
authors: Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. jumagdalon@ig.com.br
authors: Juliana
authors: J
authors: Magdalon
authors: Elaine
authors: E
authors: Hatanaka
authors: Talita
authors: T
authors: Romanatto
authors: Hosana G
authors: HG
authors: Rodrigues
authors: Wilson Mt
authors: WM
authors: Kuwabara
authors: Caitriona
authors: C
authors: Scaife
authors: Philip
authors: P
authors: Newsholme
authors: Rui
authors: R
authors: Curi
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation; D005227:Fatty Acids / Q000494:pharmacology* / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D041681:NIH 3T3 Cells; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; C489428:Myc protein, mouse; D020543:Proteome; D016271:Proto-Oncogene Proteins c-myc; D006597:Fructose-Bisphosphatase; D010751:Phosphopyruvate Hydratase