title: Poly(L-lactic acid) nanocylinders as nanofibrous structures for macroporous gelatin scaffolds.
abstract: Electrospun Nanofiber sheets have been shown to mimic the structure of extracellular matrix (ECM). Although these nanofibers have shown great potential for use as tissue engineering scaffolds, it is difficult for the electrospun nanofiber based sheets to be shaped into the desired three-dimensional structure. In this study, poly(L-lactic acid) (PLLA), a biodegradable and biocompatible polyester, was electrospun to produce nanofibers that were treated with an amino group containing base in order to fabricate polymeric nanocylinders. The aspect ratio of the PLLA nanocylinders was tunable by varying the aminolysis time and density of the amino group containing base. The effects of changes in nanofibrous morphology of the PLLA nanocylinders/macro-porous gelatin scaffolds on cell adhesion and proliferation were evaluated. The results revealed different cell morphology, adhesion, and proliferation in the nanocylinders composite gelatin scaffold versus gelatin scaffold alone. Confocal laser scanning microscopy observation showed more spreading and a more flattened cell morphology after NIH3T3 cells were cultured on PLLA nanocylinders/gelatin scaffolds for 10 hours and 4 days. These results indicate that the gelatin/PLLA nanocylinder composite is a promising way to fabricate 3D nanofibrous scaffolds that accelerates cell adhesion and proliferation for tissue engineering.
authors: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, 130-701, Republic of Korea.
authors: Jung Bok
authors: JB
authors: Lee
authors: Sung In
authors: SI
authors: Jeong
authors: Min Su
authors: MS
authors: Bae
authors: Dong Nyoung
authors: DN
authors: Heo
authors: Jung Sun
authors: JS
authors: Heo
authors: Yu-Shik
authors: YS
authors: Hwang
authors: Hyeon-Woo
authors: HW
authors: Lee
authors: Il Keun
authors: IK
authors: Kwon
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D057139:Nanofibers; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005780:Gelatin