title: Genetically encoded photocrosslinkers as molecular probes to study G-protein-coupled receptors (GPCRs).
abstract: The genetic code was expanded with orthogonal pairs to introduce photoactivatable amino acids into G-protein-coupled receptors (GPCRs) in a noninvasive manner. In this way the receptor surface could be mapped by searching for specific ligand interaction sites and the complex dynamics could be studied. This method is also useful for probing the structure of GPCR complexes in living cells.
authors: AK Biochemie und Biooranische Chemie, Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103 Leipzig, Germany. beck-sickinger@uni-leipzig.de
authors: Annette G
authors: AG
authors: Beck-Sickinger
authors: Nediljko
authors: N
authors: Budisa
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D055668:Photochemical Processes; D011487:Protein Conformation; D015202:Protein Engineering; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003432:Cross-Linking Reagents; D008024:Ligands; D043562:Receptors, G-Protein-Coupled