title: Immunological features and the ability of inhibitory effects on enzymatic activity of an epitope vaccine composed of cholera toxin B subunit and B cell epitope from Helicobacter pylori urease A subunit.
abstract: Epitope vaccine based on urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In this study, we constructed an epitope vaccine with mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA(183-203)) of H. pylori urease A subunit named CTB-UA. The CTB-UA fusion protein was expressed in Escherichia coli, and the purified protein was used for intraperitoneal immunization experiments in BALB/c mice. The experimental results indicated that anti-CTB-UA antibody could recognize both H. pylori urease A subunit (UreA) and urease B subunit (UreB). Besides, the CTB-UA epitope vaccine had good immunogenicity and immunoreactivity and could induce specific neutralizing antibodies which showed effectively inhibitory effect on the enzymatic activity of H. pylori urease. CTB-UA is a promising molecule to be investigated as H. pylori vaccine antigen candidate.
authors: Biotechnology Center, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing, 210009, China. GUOLETIAN1982@163.com
authors: Le
authors: L
authors: Guo
authors: Xiaokang
authors: X
authors: Li
authors: Feng
authors: F
authors: Tang
authors: Yunmian
authors: Y
authors: He
authors: Yingying
authors: Y
authors: Xing
authors: Xuepeng
authors: X
authors: Deng
authors: Tao
authors: T
authors: Xi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D016481:Helicobacter Infections / Q000517:prevention & control; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D014510:Urease / Q000037:antagonists & inhibitors* / Q000276:immunology*; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D001428:Bacterial Vaccines; D018985:Epitopes, B-Lymphocyte; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D002772:Cholera Toxin; D014510:Urease