title: Numerical fidelity of endoscopic biopsy fragments in the processing sequence of a university surgical pathology laboratory.
abstract: BACKGROUND
  Diagnostic specimens in surgical pathology are, in general, becoming smaller and smaller, as minimally invasive surgical procedures are used to obtain representative tissue. Conservation and effective utilization of small biopsy tissue is therefore crucial in the pathology laboratory.
  
  
  OBJECTIVE
  To identify potential areas where biopsy tissue may be lost in the course of processing in our university-based laboratory.
  
  
  METHODS
  We followed 2934 endoscopic biopsy samples as they moved through our grossing area and histology laboratory by documenting the number of fragments inked and placed within tissue paper at the time of gross assessment, the number of fragments found in each cassette after processing and subsequently embedded in paraffin, and the number of pieces of tissue present on glass slides after staining.
  
  
  RESULTS
  In 805 (27.4%) cases, the number of fragments of tissue noted on glass slides containing 2 levels of the paraffin block differed from the number submitted in the tissue cassette. Of these, most (137, 17%) differed between the number of fragments embedded in paraffin and those identified on glass slides. Loss of tissue fragments occurred in only 7.2% of cases, while 26.3% had gains in tissue fragments.
  
  
  CONCLUSIONS
  Recognition of type(s) and source(s) of variation in biopsy fragment numbers is important in quality control and in the overall practical management of a histology laboratory.
authors: Department of Pathology, University of Pittsburgh Medical Center, Presbyterian Campus, Pennsylvania 15213-2582, USA.
authors: Scott R
authors: SR
authors: Owens
authors: Luke
authors: L
authors: Wiehagen
authors: Christopher
authors: C
authors: Simmons
authors: Alena
authors: A
authors: Sikorova
authors: William
authors: W
authors: Stewart
authors: Susan
authors: S
authors: Kelly
authors: Richard
authors: R
authors: Nestler
authors: Samuel A
authors: SA
authors: Yousem
mesh_terms: D001706:Biopsy / Q000592:standards*; D004724:Endoscopy / Q000592:standards*; D006652:Histological Techniques / Q000592:standards; D006801:Humans; D007753:Laboratories / Q000592:standards*; D010340:Pathology, Surgical / Q000592:standards; D010414:Pennsylvania; D011786:Quality Control; D013048:Specimen Handling / Q000592:standards; D014495:Universities