title: The role of a commercial enzyme immuno assay antigen detection system for diagnosis of C. trachomatis in genital swab samples.
abstract: In the present pilot study, endocervical and urethral swabs collected from 100 patients attending sexually transmitted disease (STD) clinics and regional centre for STD in two referral hospitals in New Delhi were analyzed by enzyme immune assay (EIA), polymerase chain reaction (PCR) and direct fluorescent antibody (DFA) for detection of C. trachomatis. It was found that EIA could detect a very low number of cases (3/100) as against DFA (11/100) and PCR (9/100). Thus, in spite of the widespread availability, lower cost and ease of performance of the enzyme-linked-immunosorbent serologic assay, the present study highlights the need to employ sophisticated diagnostic tools like DFA and PCR for detection of Chlamydia trachomatis in STD patients.
authors: Department of Microbiology, Dr. RP Centre for Ophthalmic Sciences, New Delhi, India.
authors: A
authors: A
authors: Mukherjee
authors: S
authors: S
authors: Sood
authors: M
authors: M
authors: Bala
authors: G
authors: G
authors: Satpathy
authors: N
authors: N
authors: Mahajan
authors: A
authors: A
authors: Kapil
authors: V K
authors: VK
authors: Sharma
mesh_terms: D000293:Adolescent; D000328:Adult; D000946:Antigens, Fungal / Q000032:analysis*; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D005835:Genitalia / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007194:India; D008219:Lymphogranuloma Venereum / Q000175:diagnosis* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000946:Antigens, Fungal