title: RNA-Seq and find: entering the RNA deep field.
abstract: Initial high-throughput RNA sequencing (RNA-Seq) experiments have revealed a complex and dynamic transcriptome, but because it samples transcripts in proportion to their abundances, assessing the extent and nature of low-level transcription using this technique has been difficult. A new assay, RNA CaptureSeq, addresses this limitation of RNA-Seq by enriching for low-level transcripts with cDNA tiling arrays prior to high-throughput sequencing. This approach reveals a plethora of transcripts that have been previously dismissed as 'noise', and hints at single-cell transcription fingerprints that may be crucial in defining cellular function in normal and disease states.
authors: Department of Computer Science, UC Berkeley, Berkeley, CA 94720, USA. lpachter@math.berkeley.edu.
authors: Adam
authors: A
authors: Roberts
authors: Lior
authors: L
authors: Pachter