title: Thickness of multiwalled carbon nanotubes affects their lung toxicity.
abstract: Two samples of highly pure multiwalled carbon nanotubes (MWCNTs) similar in hydrophobicity and surface reactivity were prepared with similar length, <5 μm, but markedly different diameter (9.4 vs 70 nm). The samples were compared for their cytotoxic activity, uptake, and ability to induce oxidative stress (ROS production and intracellular GSH depletion) in vitro in murine alveolar macrophages (MH-S). The in vivo toxicity was evaluated by measuring biochemical (LDH activity and total proteins) and cellular responses in bronchoalveolar lavage (BAL) after intratracheal instillation in rats. Both samples were internalized in MH-S cells. However, thin MWCNTs appeared significantly more toxic than the thicker ones, both in vitro and in vivo, when compared on a mass-dose basis. The data reported herein suggest that the nanotube diameter is an important parameter to be considered in the toxicological assessment of CNTs.
authors: Dipartimento di Chimica, Università degli Studi di Torino , via P. Giuria 7, Torino 10125, Italy.
authors: Ivana
authors: I
authors: Fenoglio
authors: Elisabetta
authors: E
authors: Aldieri
authors: Elena
authors: E
authors: Gazzano
authors: Federico
authors: F
authors: Cesano
authors: Massimiliano
authors: M
authors: Colonna
authors: Domenica
authors: D
authors: Scarano
authors: Gianna
authors: G
authors: Mazzucco
authors: Angelo
authors: A
authors: Attanasio
authors: Yousof
authors: Y
authors: Yakoub
authors: Dominique
authors: D
authors: Lison
authors: Bice
authors: B
authors: Fubini
mesh_terms: D000818:Animals; D001692:Biological Transport; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D007770:L-Lactate Dehydrogenase; D005978:Glutathione