title: Green and efficient production of octyl hydroxyphenylpropionate using an ultrasound-assisted packed-bed bioreactor.
abstract: A solvent-free system to produce octyl hydroxyphenylpropionate (OHPP) from p-hydroxyphenylpropionic acid (HPPA) and octanol using immobilized lipase (Novozym® 435) as a catalyst in an ultrasound-assisted packed-bed bioreactor was investigated. Response-surface methodology (RSM) and a three-level-three-factor Box-Behnken design were employed to evaluate the effects of reaction temperature (x₁), flow rate (x₂) and ultrasonic power (x₃) on the percentage of molar production of OHPP. The results indicate that the reaction temperature and flow rate were the most important variables in optimizing the production of OHPP. Based on a ridge max analysis, the optimum conditions for OHPP synthesis were predicted to consist of a reaction temperature of 65°C, a flow rate of 0.05 ml/min and an ultrasonic power of 1.74 W/cm² with a yield of 99.25%. A reaction was performed under these optimal conditions, and a yield of 99.33 ± 0.1% was obtained.
authors: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors: Chih Chen
authors: CC
authors: Lee
authors: Hsiao Ching
authors: HC
authors: Chen
authors: Hen Yi
authors: HY
authors: Ju
authors: Jiann Hwa
authors: JH
authors: Chen
authors: Chia Hung
authors: CH
authors: Kuo
authors: Yi Lin
authors: YL
authors: Chung
authors: Yung Chuan
authors: YC
authors: Liu
authors: Chwen Jen
authors: CJ
authors: Shieh
mesh_terms: D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000378:metabolism*; D019220:High-Energy Shock Waves; D008049:Lipase / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D010666:Phenylpropionates / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D004800:Enzymes, Immobilized; D010636:Phenols; D010666:Phenylpropionates; D011422:Propionates; C498246:octyl hydroxyphenylpropionate; C008869:phloretic acid; D008049:Lipase