title: Metabolic engineering of Escherichia coli for 1-butanol biosynthesis through the inverted aerobic fatty acid β-oxidation pathway.
abstract: The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as enzymes from other metabolic pathways. In the current study, we demonstrate the inversion of the entire aerobic fatty acid β-oxidation cycle for 1-butanol biosynthesis. The constructed markerless and plasmidless Escherichia coli strain BOX-3 (MG1655 lacI(Q) attB-P(trc-ideal-4)-SD(φ10)-adhE(Glu568Lys) attB-P(trc-ideal-4)-SD(φ10)-atoB attB-P(trc-ideal-4)-SD(φ10)-fadB attB-P(trc-ideal-4)-SD(φ10)-fadE) synthesises 0.3-1 mg 1-butanol/l in the presence of the specific inducer. No 1-butanol production was detected in the absence of the inducer.
authors: Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhniy Pr., 1, Moscow, Russia 117545. gulevich@genetika.ru
authors: Andrey Yu
authors: AY
authors: Gulevich
authors: Alexandra Yu
authors: AY
authors: Skorokhodova
authors: Alexey V
authors: AV
authors: Sukhozhenko
authors: Rustem S
authors: RS
authors: Shakulov
authors: Vladimir G
authors: VG
authors: Debabov
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000332:Aerobiosis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D010084:Oxidation-Reduction
chemical_list: D005227:Fatty Acids; D020001:1-Butanol