title: Quantification of amino acids in a single cell by microchip electrophoresis with chemiluminescence detection.
abstract: Analyzing individual cells allows detecting a minor group of abnormal cells present in a large population of normal cells. This ability can be essential to understanding diseases, such as cancer and diabetes. Microchip electrophoresis (MCE) is the technique of choice for single-cell analysis. However, since the channels in microfluidic devices are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe an MCE method with highly sensitive chemiluminescence detection for simultaneous determination of multiple amino acids present in single cells.
authors: Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA. yiming.liu@jsums.edu
authors: Yi-Ming
authors: YM
authors: Liu
authors: Shulin
authors: S
authors: Zhao
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002138:Calibration; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D049449:Luminescence; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D000596:Amino Acids