title: Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies.
abstract: Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.
authors: The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.
authors: Vardis
authors: V
authors: Ntoukakis
authors: Benjamin
authors: B
authors: Schwessinger
authors: Cécile
authors: C
authors: Segonzac
authors: Cyril
authors: C
authors: Zipfel
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D060406:Brassinosteroids / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D010444:Peptide Elongation Factor Tu / Q000378:metabolism / Q000494:pharmacology; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D011993:Recombinant Fusion Proteins; D005408:Flagellin; D011494:Protein Kinases; C463660:BAK1 protein, Arabidopsis; C411853:FLS2 protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D010444:Peptide Elongation Factor Tu