title: Single-molecule transcript counting of stem-cell markers in the mouse intestine.
abstract: Determining the molecular identities of adult stem cells requires technologies for sensitive transcript detection in tissues. In mouse intestinal crypts, lineage-tracing studies indicated that different genes uniquely mark spatially distinct stem-cell populations, residing either at crypt bases or at position +4, but a detailed analysis of their spatial co-expression has not been feasible. Here we apply three-colour single-molecule fluorescent in situ hybridization to study a comprehensive panel of intestinal stem-cell markers during homeostasis, ageing and regeneration. We find that the expression of all markers overlaps at crypt-base cells. This co-expression includes Lgr5, Bmi1 and mTert, genes previously suggested to mark distinct stem cells. Strikingly, Dcamkl1 tuft cells, distributed throughout the crypt axis, co-express Lgr5 and other stem-cell markers that are otherwise confined to crypt bases. We also detect significant changes in the expression of some of the markers following irradiation, indicating their potential role in the regeneration process. Our approach can enable the sensitive detection of putative stem cells in other tissues and in tumours, guiding complementary functional studies to evaluate their stem-cell properties.
authors: Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors: Shalev
authors: S
authors: Itzkovitz
authors: Anna
authors: A
authors: Lyubimova
authors: Irene C
authors: IC
authors: Blat
authors: Mindy
authors: M
authors: Maynard
authors: Johan
authors: J
authors: van Es
authors: Jacqueline
authors: J
authors: Lees
authors: Tyler
authors: T
authors: Jacks
authors: Hans
authors: H
authors: Clevers
authors: Alexander
authors: A
authors: van Oudenaarden
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006706:Homeostasis / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D007422:Intestines / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; C489421:Bmi1 protein, mouse; C493597:Lgr5 protein, mouse; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D012097:Repressor Proteins; D063150:Polycomb Repressive Complex 1; D019098:Telomerase; C509187:Tert protein, mouse