title: Diagnostic value of markers of muscle degeneration in sporadic inclusion body myositis.
abstract: Sporadic inclusion body myositis (s-IBM) is characterized histologically by the association of concomitant inflammatory and degenerative processes. We evaluated the sensitivity and specificity of different markers of the degenerative process in order to refine the histological diagnosis. We performed an immunohistochemical study with antibodies directed against ubiquitin, amyloid-beta precursor protein (AbetaPP), amyloid-beta (Abeta), SMI-31, SMI-310, Tar-DNA binding protein-43 (TDP-43) and p62 on s-IBM and control muscle biopsies. Based on conventional stains 36 patients with characteristic clinical features of s-IBM were subclassified as presumed definite s-IBM (d s-IBM, n = 17) or possible s-IBM (p s-IBM, n = 19) according to the presence or absence of vacuolated muscle fibers. Immunohistochemically, TDP-43 and p62 were the most sensitive markers, accumulating in all d s-IBM and in 31% and 37%, respectively, of the p s-IBM cases and thus enabling reclassification of these cases as d s-IBM. We recommend using TDP-43 and p62 antibodies in the histological diagnosis workup of s-IBM. The specificity of these markers has to be further validated in prospective series.
authors: Laboratoire de Neuropathologie, Institut de Myologie, Assistance Publique, Hôpitaux de Paris, Hôpital Pitié-Salpêtrière, Université Pierre et Marie Curie, Paris, France. odile.dubourg@psl.aphp.fr
authors: O
authors: O
authors: Dubourg
authors: J
authors: J
authors: Wanschitz
authors: T
authors: T
authors: Maisonobe
authors: A
authors: A
authors: Béhin
authors: Y
authors: Y
authors: Allenbach
authors: S
authors: S
authors: Herson
authors: O
authors: O
authors: Benveniste
mesh_terms: D015415:Biomarkers; D001706:Biopsy; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology; D018979:Myositis, Inclusion Body / Q000175:diagnosis / Q000378:metabolism; D018579:Patient Selection
chemical_list: D015415:Biomarkers; D003598:Cytoskeletal Proteins