title: Real-time optical gating for three-dimensional beating heart imaging.
abstract: We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We also present initial results demonstrating the application of this technique to 3-D structural imaging of the beating embryonic heart.
authors: Durham University, Centre for Advanced Instrumentation, Department of Physics, Durham, United Kingdom. j.m.taylor@durham.ac.uk
authors: Jonathan M
authors: JM
authors: Taylor
authors: Christopher D
authors: CD
authors: Saunter
authors: Gordon D
authors: GD
authors: Love
authors: John M
authors: JM
authors: Girkin
authors: Deborah J
authors: DJ
authors: Henderson
authors: Bill
authors: B
authors: Chaudhry
mesh_terms: D000465:Algorithms; D000818:Animals; D004625:Embryo, Nonmammalian; D006321:Heart / Q000033:anatomy & histology / Q000196:embryology* / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007734:Kymography; D008856:Microscopy, Fluorescence / Q000379:methods; D009200:Myocardial Contraction / Q000502:physiology; D015027:Zebrafish