title: Anticancer effect and feasibility study of hyperthermia treatment of             pancreatic cancer using magnetic nanoparticles.
abstract: We investigated the effect and feasibility of hyperthermia treatment on             subcutaneous pancreatic cancer in female Kunming mice, using a murine pancreatic             cancer cell line (MPC-83) established by us and found in this study to originate             from epithelial pancreatic acinus. Magnetic fluid (MF) with ferromagnetic particles             of about 20 nm in size was used as a heating mediator. MF was injected into the             subcutaneous nodules with subaxillary regions of mice 10 days after tumor transplantation;             homogeneous distribution of magnetic nanoparticles in nodules was easily detected             by X-ray 24 h later. Mice were allocated to four groups as follows: no treatment             (control); MF injection alone; alternating magnetic field (AMF) irradiation alone;             and MF injection and hyperthermia generated by applying AMF (300 kHz, 110 Gs).             The two hyperthermia-treated subgroup tumors reached central temperatures of 47             and 51˚C, respectively, for 30 min; while rectal temperature in both subgroups             remained below 36˚C. Tumor growth was inhibited and survival significantly prolonged             in the hyperthermia group compared with other groups (P<0.05). Tumor cells             near the MF in the hyperthermia group apoptosed or necrosed immediately after             hyperthermia. By day 14, there were no subcutaneous nodules; and residual magnetic             nanoparticles were ingested by phagocytes. Nuclear proliferating cell nuclear             antigen (PCNA) decreased in hyperthermia group tumor cells compared to the other             groups; cytoplasmic heat shock protein 70 (HSP 70) was conspicuously higher immediately             after hyperthermia (P<0.05). This technique had therapeutic potential and provided             a new idea in the treatment of pancreatic cancer.
authors: Department of Biotherapy, The First Affiliated Hospital of Kunming University of Medical Science, Kunming 650032, PR China.
authors: Lufang
authors: L
authors: Wang
authors: Jian
authors: J
authors: Dong
authors: Weiwei
authors: W
authors: Ouyang
authors: Xiaowen
authors: X
authors: Wang
authors: Jintian
authors: J
authors: Tang
mesh_terms: D061354:Acinar Cells / Q000378:metabolism / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006979:Hyperthermia, Induced / Q000379:methods*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D059346:Magnets; D051379:Mice; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013534:Survival; D013696:Temperature; D014018:Tissue Distribution
chemical_list: D018840:HSP70 Heat-Shock Proteins; D058185:Magnetite Nanoparticles; D018809:Proliferating Cell Nuclear Antigen