title: Ultra-sonication-assisted solvent extraction of quercetin glycosides from 'Idared' apple peels.
abstract: Quercetin and quercetin glycosides are physiologically active flavonol molecules that have been attributed numerous health benefits. Recovery of such molecules from plant matrices depends on a variety of factors including polarity of the extraction solvent. Among the solvents of a wide range of dielectric constants, methanol recovered the most quercetin and its glycosides from dehydrated 'Idared' apple peels. When ultra-sonication was employed to facilitate the extraction, exposure of 15 min of ultrasound wavelengths of dehydrated apple peel powder in 80% to 100% (v/v) methanol in 1:50 (w:v) solid to solvent ratio provided the optimum extraction conditions for quercetin and its glycosides. Acidification of extraction solvent with 0.1% (v/v) or higher concentrations of HCl led to hydrolysis of naturally occurring quercetin glycosides into the aglycone as an extraction artifact.
authors: Tree Fruit Bio-product Research Program, Department of Environmental Sciences, Nova Scotia Agricultural College, P.O. Box 550, Truro, Nova Scotia, B2N 5E3, Canada. vrupasinghe@nsac.ca
authors: H P
authors: HP
authors: Vasantha Rupasinghe
authors: Priya
authors: P
authors: Kathirvel
authors: Gwendolyn M
authors: GM
authors: Huber
mesh_terms: D006027:Glycosides / Q000302:isolation & purification*; D006851:Hydrochloric Acid / Q000737:chemistry; D027845:Malus / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification*; D011794:Quercetin / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*; D014866:Waste Products
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D012997:Solvents; D014866:Waste Products; D011794:Quercetin; D006851:Hydrochloric Acid; D000432:Methanol