title: The effect of 2,5-hexanedione on myelin protein zero expression, and its mitigation using Ginkgo biloba extract.
abstract: OBJECTIVE
  To investigate the role of myelin protein zero (P(0)) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury, and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.
  
  
  METHODS
  After 4 weeks of treatment with 2,5-HD at different doses (50, 100, 200, 400 mg/kg) in rats, changes in the levels of P(0) in rat sciatic nerves was investigated, and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.
  
  
  RESULTS
  The blood-nerve barrier (BNB) permeability of the sciatic nerve increased, and the expression of P(0) mRNA and P(0) protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks. Pretreatment with Egb761 protected against BNB interruption, and inhibited P(0) mRNA and protein reduction during 2,5-HD treatment. Pretreatment with Egb761 significantly reduced loss of body weight (P<0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P<0.01). It also reduced the signs of neurotoxicity induced by 2,5-HD.
  
  
  CONCLUSIONS
  2,5-HD inhibited the expression of P(0) in a dose-dependent manner, and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD. Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.
authors: National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors: Lei
authors: L
authors: Zhao
authors: QingJun
authors: Q
authors: Liu
authors: Hong
authors: H
authors: Chen
authors: HuaWei
authors: H
authors: Duan
authors: Ping
authors: P
authors: Bin
authors: Qing
authors: Q
authors: Liu
authors: Yong
authors: Y
authors: Niu
authors: YuFei
authors: Y
authors: Dai
authors: YuXin
authors: Y
authors: Zheng
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity; D005786:Gene Expression Regulation / Q000187:drug effects*; D020441:Ginkgo biloba; D006588:Hexanones / Q000633:toxicity*; D008297:Male; D018993:Myelin P0 Protein / Q000235:genetics / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects; D000096983:Ginkgo Extract
chemical_list: D004785:Environmental Pollutants; D006588:Hexanones; D018993:Myelin P0 Protein; D018696:Neuroprotective Agents; D010936:Plant Extracts; D012333:RNA, Messenger; C560548:Mpz protein, rat; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C011269:2,5-hexanedione