title: A strong promoter, PMagpd, provides a tool for high gene expression in entomopathogenic fungus, Metarhizium acridum.
abstract: A glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter (PMagpd) was obtained from Metarhizium acridum and its active region analyzed by 5'-deletion strategy using β-glucuronidase (GUS) as a reporter. Sequence analysis revealed that typical regulatory elements of PMagpd were included in the 1.7 kb region upstream of the start codon of the Magpd gene. Deletion of the region from -1,691 bp to -1,463 bp, where the gpd box is harbored, did not significantly affect the PMagpd activity. Deletions of the regions upstream of -946 bp and upstream of -684 bp caused a major decrease of GUS activity. Compared with PgpdA (2.2 kb) in Aspergillus nidulans, PMagpd (1.4 kb) had a shorter sequence and significantly higher activity in M. acridum. This study provides an applicable promoter for over-expression of target genes in M. acridum.
authors: Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Chongqing 400030, People's Republic of China. yueqingcao@126.com
authors: Yueqing
authors: Y
authors: Cao
authors: Run
authors: R
authors: Jiao
authors: Yuxian
authors: Y
authors: Xia
mesh_terms: D019677:Artificial Gene Fusion; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D004252:DNA Mutational Analysis; D015870:Gene Expression; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D052981:Metarhizium / Q000201:enzymology / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion
chemical_list: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005966:Glucuronidase