title: Effect of total body X-ray irradiation on lymph node in Tibet minipig.
abstract: The purpose of this study was to determine the time-dose-effect of total body X-ray irradiation on lymphocytes in lymph nodes and peripheral blood in Tibet minipigs. Forty-eight Tibet minipigs were assigned into 6 groups including 5 experimental groups with 9 and the control group with 3. The minipigs in experimental groups were subjected to a total body X-ray irradiation of 2, 5, 8, 11, and 14 Gy respectively. Lymph nodes and peripheral blood samples were collected at 6, 24, and 72 hours after X-ray exposure and received histological microscopy examination and apoptosis analysis. Histology observation showed that the number of lymphocytes decreased within the lymph nodes with the increase of radiation doses and exposure time. The observation of transmission electron microscopy (TEM) showed typical apoptotic cells below 11 Gy while at 14 Gy necrotic cells were dominant. The apoptotic rate of lymphocytes in the lymph nodes was positively correlated with radiation dose in the range of 2-11 Gy, and reached the maximal level (39.4 ± 2.8) at 24 hours after 11 Gy irradiation, followed by a decrease in the apoptotic rate, but still higher than that of the control group. The number of lymphocytes in the peripheral blood samples was decreased significantly by increasing of the radiation dose and exposure time. We conclude that early damage of lymphocytes by total body X-ray irradiation is dose and time dependent below 11 Gy and before 24 hours post irradiation, and that the dosage of irradiation less than 11 Gy induced apoptosis, whereas the dose at 14 Gy resulted in necrosis in lymphocytes of the lymph nodes.
authors: Department of Hemotology, Zhujiang Hospital, Southern Medical University, Guangdong, China.
authors: Shao-Jie
authors: SJ
authors: Wu
authors: Yu-Jue
authors: YJ
authors: Wang
authors: Kun-Yuan
authors: KY
authors: Guo
authors: Chi
authors: C
authors: Chen
authors: Tong-Feng
authors: TF
authors: Zhao
authors: Mao-Ben
authors: MB
authors: Sun
authors: Wei-Wang
authors: WW
authors: Gu
authors: Ying Ying
authors: YY
authors: Gao
authors: Hui Juan
authors: HJ
authors: Han
authors: Fei
authors: F
authors: Zou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001773:Blood Cells / Q000528:radiation effects / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D008198:Lymph Nodes / Q000528:radiation effects*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D023421:Models, Animal; D009336:Necrosis; D011836:Radiation Tolerance; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000009:adverse effects