title: Ultraviolet-B irradiation induces epidermal up-regulation of heparanase expression and activity.
abstract: Heparan sulfate (HS) glycosaminoglycans are abundant components of basement membranes and cell surfaces where they are present associated with specific core-proteins to form proteoglycans, mainly perlecan, glypicans and syndecans. They play many roles such as modulation of cell proliferation and differentiation, cell-matrix adhesion and assembly. It was previously shown that HS content decreases during skin aging. This decrease could be explained either by a decrease of HS synthesis or by an increased activity of its degrading enzyme, heparanase (Hpse-1). Since UV-B irradiation is one of the most important factor for skin photo-damage, we decided to study the effects of UV-B irradiation on heparanase expression and activity in human epidermal keratinocytes. Normal human keratinocytes and reconstructed epidermis were submitted to increasing doses of UV-B. HPSE1 mRNA levels were measured using real time PCR and heparanase enzymatic activity was quantified in human keratinocyte cultures using a microtiter-based assay. Expression and distribution of Hpse-1 were also studied in reconstructed epidermis by immunofluorescence. Both HPSE1 mRNA level and heparanase enzymatic activity were increased after UV-B irradiation of keratinocyte cultures in a time and dose-dependent manner. Protein expression of Hpse-1 was also up-regulated with increasing doses of UV-B in reconstructed epidermis. Increase of Hpse-1 expression and activity in the epidermis after UV-B irradiation could contribute to skin photo-aging.
authors: Université de Reims Champagne-Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, CNRS UMR 6237, 51 rue Cognacq Jay, 51095 Reims cedex, France.
authors: Sandrine
authors: S
authors: Kurdykowski
authors: Solène
authors: S
authors: Mine
authors: Vincent
authors: V
authors: Bardey
authors: Louis
authors: L
authors: Danoux
authors: Christine
authors: C
authors: Jeanmaire
authors: Gilles
authors: G
authors: Pauly
authors: Eva
authors: E
authors: Brabencova
authors: Yanusz
authors: Y
authors: Wegrowski
authors: François-Xavier
authors: FX
authors: Maquart
mesh_terms: D002460:Cell Line; D004817:Epidermis / Q000528:radiation effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays; D015854:Up-Regulation; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D012333:RNA, Messenger; D000099175:Heparanase