title: Cell death forms and HSP70 expression in U87 cells after ionizing radiation and/or chemotherapy.
abstract: BACKGROUND
  Several types of cell death can induce the activation of the immune system by releasing factors of damage.
  
  
  OBJECTIVE
  To identify different cell death modalities using U87 glioblastoma cell line after radio-chemotherapy treatments by analyzing the expression of HSP70 after γ-ray irradiation and temozolomide treatment.
  
  
  METHODS
  U87 cell line was irradiated with 2, 4, 8, 10 and 20 Gy, treated with 200 μM of temozolomide, or subjected to combined treatments of these.
  
  
  RESULTS
  Forty-eight hours after treatment, apoptosis was more prevalent than necrosis, especially after γ-radiation with a somewhat dose-dependent trend. Temozolomide did not seem to induce significant modifications of the pattern of cell death and protein expression. Temozolomide-induced cell death was lower or equal to that obtained with high dose radiotherapy. A low synergism between the two treatments was observed.
  
  
  CONCLUSIONS
  This cell line showed a marked radioresistance to conventional clinical doses, showing attempts to repair the induced damage, while temozolomide treatment showed no cytotoxic effects.
authors: Department of Animal Biology, University of Pavia, Via Ferrata 9, I-27100 Pavia, Italy.
authors: Alessandro
authors: A
authors: Paolini
authors: Francesca
authors: F
authors: Pasi
authors: Angelica
authors: A
authors: Facoetti
authors: Giuliano
authors: G
authors: Mazzini
authors: Franco
authors: F
authors: Corbella
authors: Riccardo
authors: R
authors: Di Liberto
authors: Rosanna
authors: R
authors: Nano
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D059248:Chemoradiotherapy; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005720:Gamma Rays; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D000077204:Temozolomide; D014407:Tumor Cells, Cultured
chemical_list: D018906:Antineoplastic Agents, Alkylating; D018840:HSP70 Heat-Shock Proteins; D003606:Dacarbazine; D000077204:Temozolomide