title: Construction and analysis of full-length and normalized cDNA libraries from citrus.
abstract: We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genes.
authors: Instituto de Biología Molecular y Celular de Plantas-IBMCP, Universidad Politécnica de Valencia-UPV and Consejo Superior de Investigaciones Científicas-CSIC, CPI 8E, Ingeniero Fausto Elio s/n, Valencia 46022, Spain.
authors: M Carmen
authors: MC
authors: Marques
authors: Miguel A
authors: MA
authors: Perez-Amador
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000138:chemical synthesis* / Q000302:isolation & purification; D015723:Gene Library; D005822:Genetic Vectors; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids / Q000235:genetics; D048348:Reverse Transcription; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary