title: Identification of Yersinia ruckeri from diseased salmonid fish by Fourier transform infrared spectroscopy.
abstract: Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM), which mainly affects salmonid fish. Isolates of Y. ruckeri from diseased salmonid fish were obtained over a 6-year period from eight fish farms in the State of Baden-Württemberg, Southwest Germany. The strains were characterized by biochemical methods and Fourier transform infrared spectroscopy (FT-IR) combined with artificial neural network analysis. These methods were complemented by 16S rDNA sequencing for several isolates. The set of strains from these fish farms included sorbitol-positive, gelatinase-positive and non-motile Y. ruckeri. These variants were differentiated with an advanced FT-IR module, which is part of a higher-ranking method including more than 200 well-defined Yersinia strains against a background of more than 1000 other Gram-negative isolates. Validation of the newly constructed method yielded 97.4% of Y. ruckeri identified correctly on the species level. Thus, the FT-IR analysis enables distinction of all Y. ruckeri from other Yersinia species (e.g. fish-borne Y. enterocolitica) and other Enterobacteriaceae typically misidentified because of similar biochemical reaction profiles, especially Hafnia alvei. The differentiation of sorbitol-positive variants of Y. ruckeri using FT-IR was demonstrated.
authors: Chemisches und Veterinäruntersuchungsamt Stuttgart (CVUAS), Fellbach, Germany.
authors: F
authors: F
authors: Wortberg
authors: E
authors: E
authors: Nardy
authors: M
authors: M
authors: Contzen
authors: J
authors: J
authors: Rau
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D005393:Fish Diseases / Q000382:microbiology*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D012487:Salmonidae / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012336:RNA, Ribosomal, 16S