title: [Stx2a-producing enteroaggregative Escherichia coli O104:H4-ST678. Microbiological diagnostic already, for this and other STEC/VTEC serotypes!].
abstract: A Stx2a-producing Escherichia coli (STEC) strain belonging to serotype O104:H4, with virulence features common to the enteroaggregative E. coli pathotype, was reported as the cause of the recent 2011 outbreak in Germany. In addition, the German outbreak strain was found to possess several virulence factors of extra-intestinal pathogenic E. coli and to have acquired resistance to numerous antibiotics, including third-generation cephalosporins, owing to several plasmid-borne genes encoding TEM-1 and CTX-M-15 β-lactamases. There are only a few reports of serotype O104:H4, which is very rare in humans, and has never been detected in animals or food. Once the serotype of the German outbreak strain became known, specific molecular methods were developed for its detection based on conventional and real-time PCR. Data from Galicia suggest that, per year in Spain, STEC O157:H7 is responsible for more than 500 cases of infection, and non-O157 for more than 2,000. A microbiological diagnosis for O104:H4, O157:H7 and other STEC serotypes is required in Spanish hospitals.
authors: Laboratorio de Referencia de E.coli, Departamento de Microbioloxía e Parasitoloxía, Universidade de Santiago de Compostela, Campus de Lugo, España. jorge.blanco@usc.es
authors: Jorge
authors: J
authors: Blanco
mesh_terms: D001431:Bacteriological Techniques; D005075:Biological Evolution; D018553:Cucumis sativus / Q000382:microbiology; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004534:Egypt; D054324:Enterohemorrhagic Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D005243:Feces / Q000382:microbiology; D005506:Food Contamination; D005516:Food Microbiology; D005858:Germany / Q000453:epidemiology; D006801:Humans; D011815:R Factors / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000382:microbiology; D012703:Serotyping; D022641:Shiga Toxin 2 / Q000032:analysis; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D013030:Spain / Q000453:epidemiology; D029910:Trigonella / Q000382:microbiology; D014774:Virulence; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D022641:Shiga Toxin 2; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1