title: [Mutations in precore/basal core promoter regions and viral polymerase in patients with chronic hepatitis B].
abstract: BACKGROUND
  Variations of C gene give rise to precore and basal core mutations, which influence HBeAg expression The antiviral treatment failure is due to the presence of variations in the gene P, which cause mutations in the viral polymerase.
  
  
  METHODS
  We performed genotyping of HBV P gene by sequencing, and gene C in patients with chronic hepatitis B over a period of five years.
  
  
  RESULTS
  A total of 75% of the patients had some mutation in precore or in basal core promoter regions, and 37% demonstrated resistance mutations to antiviral treatment. The most frequent genotypes were A and D.
  
  
  CONCLUSIONS
  The presence of mutations in core/precore regions in patients with chronic hepatitis has led to tighter control of these patients. Detecting for resistance should be done as quickly as possible before there is an increase in viral load.
authors: Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, España.
authors: Ainhoa
authors: A
authors: Gutiérrez
authors: Isabel
authors: I
authors: Viciana
authors: Francisca
authors: F
authors: Rius
authors: Alfonso
authors: A
authors: Pinedo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D015897:Comorbidity; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005814:Genes, Viral; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006513:Hepatitis B e Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D017354:Point Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011446:Prospective Studies; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase