title: Pattern recognition receptors involved in the inflammatory attenuating effects of soybean isoflavone in β-amyloid peptides 1-42 treated rats.
abstract: Pattern recognition receptors (PRRs) play important roles in the inflammatory responses to Alzheimer's disease (AD). Our previous study indicated that soybean isoflavone (SIF) exhibited anti-inflammatory effect in rats treated by β-amyloid peptides1-42 (Aβ1-42). In present study, we further detected the effects of SIF against inflammation caused by Aβ1-42 treatment in rats. Serum inflammatory mediators and neurotrophic factors including transforming growth factor-β (TGF-β), inducible nitric oxide synthase (iNOS), brain-derived neurotrophic factor (BDNF) and S100β were detected by enzyme-like immunosorbent assay (ELISA). Reverse transcription-polymerase chain reaction (RT-PCR) and western blot methods were applied for detecting mRNA and protein expression of interleukin-1β (IL-1β), iNOS, tumor necrosis factor-α (TNF-α), TGF-β, BDNF, S100β, myeloid differentiation factor88 (Myd88), Toll-like receptor2 (TLR2), formyl peptide receptors (FPRs), inhibitor κB kinase (IKK) and inhibitor κB-α (IκB-α) in rat's brain tissue. Our results indicated that SIF could reduce the production of IL-1β, TNF-α and iNOS induced by Aβ1-42 in serum and brain of rats. SIF also significantly reversed Aβ1-42-induced up-regulation of TLR2, FPR, Myd88, IKK and decreased IκB-α mRNA and protein expressions in rats. These results suggested that TLR2 and FPR might involve in the inflammatory process induced by Aβ1-42 treatment, and SIF was an efficiency compound in reversing the inflammation caused by Aβ1-42 treatment.
authors: Department of Nutrition and Food Hygiene, Capital Medical University, Beijing, PR China.
authors: Linhong
authors: L
authors: Yuan
authors: Xin
authors: X
authors: Zhou
authors: Dan
authors: D
authors: Li
authors: Weiwei
authors: W
authors: Ma
authors: Huanling
authors: H
authors: Yu
authors: Yuandi
authors: Y
authors: Xi
authors: Rong
authors: R
authors: Xiao
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D044042:Receptors, Formyl Peptide / Q000096:biosynthesis; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000737:chemistry*; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis
chemical_list: D016229:Amyloid beta-Peptides; D007529:Isoflavones; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D044042:Receptors, Formyl Peptide; D051192:Receptors, Pattern Recognition; C495343:Tlr2 protein, rat; D051195:Toll-Like Receptor 2; C075222:amyloid beta-protein (1-42)