title: Interaction of single viruslike particles with vesicles containing glycosphingolipids.
abstract: Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.
authors: Department of Applied Physics, Chalmers University of Technology, S-412 96 Göteborg, Sweden.
authors: M
authors: M
authors: Bally
authors: A
authors: A
authors: Gunnarsson
authors: L
authors: L
authors: Svensson
authors: G
authors: G
authors: Larson
authors: V P
authors: VP
authors: Zhdanov
authors: F
authors: F
authors: Höök
mesh_terms: D006028:Glycosphingolipids / Q000378:metabolism*; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D013997:Time Factors; D014771:Virion / Q000378:metabolism*
chemical_list: D006028:Glycosphingolipids; D008051:Lipid Bilayers