title: Characterization of long transcribed microsatellites in Helianthus annuus (Asteraceae).
abstract: OBJECTIVE
  Research on the evolutionary role of exonic microsatellites currently lacks an understanding of the evolutionary pressures that promote or limit their expansion. Contrasting levels of variability and genetic structures at anonymous and transcribed microsatellite loci of varying lengths are likely to provide useful insights regarding the relative strength of selection acting on different classes of microsatellites. We have developed primers for long transcribed microsatellites in Helianthus annuus to make these comparisons.
  
  
  RESULTS
  Eight relatively long microsatellites from sequences in the expressed sequence tag database of H. annuus were characterized. A total of 63 individuals from three populations in Kansas were genotyped. The number of alleles per locus ranged from four to 11 with an average observed heterozygosity of 0.723.
  
  
  CONCLUSIONS
  Our study has generated suitable tools for studying the population genetics of long transcribed microsatellites that are potentially influenced by selection.
authors: Department of Biological Sciences, Mississippi State University, Starkville, 39762, USA. sp456@msstate.edu
authors: Sreepriya
authors: S
authors: Pramod
authors: Armed B
authors: AB
authors: Rasberry
authors: Tiffini G
authors: TG
authors: Butler
authors: Mark E
authors: ME
authors: Welch
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D005828:Genetics, Population; D006368:Helianthus / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D059372:Nucleotide Motifs / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers