title: High-salt-tolerance matrix for facile detection of glucose in rat brain microdialysates by MALDI mass spectrometry.
abstract: Due to its strong ultraviolet absorption, high salt tolerance, and little interference in the low molecular weight region, N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) has been applied as a matrix to measure the level of glucose in rat brain microdialysates by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in combination with in vivo microdialysis. By monitoring the ion signals of (glucose + Cl)(-) in the mass spectra, we achieved a low detection limit of ~10 μM for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification. It is concluded that NEDC-assisted laser desorption/ionization (LDI) MS is a fast and general method for sensitive detection of small molecules (such as glucose and amino acids) in high ionic strength solutions.
authors: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100190, China.
authors: Rui
authors: R
authors: Chen
authors: Wenjun
authors: W
authors: Xu
authors: Caiqiao
authors: C
authors: Xiong
authors: Xiaoyu
authors: X
authors: Zhou
authors: Shaoxiang
authors: S
authors: Xiong
authors: Zongxiu
authors: Z
authors: Nie
authors: Lanqun
authors: L
authors: Mao
authors: Yi
authors: Y
authors: Chen
authors: Huan-Cheng
authors: HC
authors: Chang
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D005947:Glucose / Q000032:analysis*; D057230:Limit of Detection; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D012965:Sodium Chloride; D005947:Glucose