title: A comparison of NCO and NCA transfer methods for biological solid-state NMR spectroscopy.
abstract: Three different techniques (adiabatic passage Hartman-Hahn cross-polarization, optimal control designed pulses, and EXPORT) are compared for transferring (15)N magnetization to (13)C in solid-state NMR experiments under magic-angle-spinning conditions. We demonstrate that, in comparison to adiabatic passage Hartman-Hahn cross-polarization, optimal control transfer pulses achieve similar or better transfer efficiencies for uniformly-(13)C,(15)N labeled samples and are generally superior for samples with non-uniform labeling schemes (such as 1,3- and 2-(13)C glycerol labeling). In addition, the optimal control pulses typically use substantially lower average RF field strengths and are more robust with respect to experimental variation and RF inhomogeneity. Consequently, they are better suited for demanding samples.
authors: Department of Chemistry, Lewis & Clark College, 0615 SW Palatine Hill Road, Portland, OR 97219, USA. loening@lclark.edu
authors: Nikolaus M
authors: NM
authors: Loening
authors: Morten
authors: M
authors: Bjerring
authors: Niels Chr
authors: NC
authors: Nielsen
authors: Hartmut
authors: H
authors: Oschkinat
mesh_terms: D000465:Algorithms; D001704:Biopolymers / Q000032:analysis* / Q000737:chemistry*; D002247:Carbon Isotopes / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D001704:Biopolymers; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes