title: Spectroscopic exploration of mode of binding of ctDNA with 3-hydroxyflavone: a contrast to the mode of binding with flavonoids having additional hydroxyl groups.
abstract: Binding interaction of 3-hydroxyflavone (3HF), a bioactive flavonoid, with calf-thymus DNA (ctDNA) has been explored exploiting various experimental techniques. The dual fluorescence of 3HF resulting from the excited state intramolecular proton transfer (ESIPT) is modified remarkably upon binding with the biomacromolecule. The determined binding constant, fluorescence quenching experiment, circular dichroism (CD) study, comparative binding study with the known intercalative binder ethidium bromide and thermometric experiment relating to the helix melting of ctDNA confirm the groove binding of 3HF with the DNA. This is in contrast to two other members of the flavonoid group, namely, fisetin and quercetin, where the bindings are established to be intercalative. The structural difference of 3HF from the other two probes with respect to the absence/presence of the additional hydroxyl groups is ascribed to be responsible for the difference in the mode of binding.
authors: Department of Chemistry, Jadavpur University, Kolkata, India.
authors: Barnali
authors: B
authors: Jana
authors: Sudipta
authors: S
authors: Senapati
authors: Debanjana
authors: D
authors: Ghosh
authors: Debosreeta
authors: D
authors: Bose
authors: Nitin
authors: N
authors: Chattopadhyay
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D005454:Fluorescence Polarization; D009691:Nucleic Acid Denaturation; D014508:Urea / Q000737:chemistry
chemical_list: D005419:Flavonoids; D014508:Urea; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; C041477:3-hydroxyflavone