title: Chemical characterization of exopolysaccharides from the marine fouling diatom amphora coffeaeformis.
abstract: Amphora coffeaeformis cells were grown in batch cultures under continuous illumination at 18°C for 10 d. Algal cells were removed by centrifugation, lyophilized and used for the extraction of exopolysaccharides using either 0.05 M EDTA, 1 M NaOH or 1.5 M NaCl. The 1.5 M NaCl treatment removed most exopolysaccharides. Glucose (81%) was the most abundant monosaccharide in the exopolysaccharides. The chemical composition data suggest that the exopolymers were acidic sulphated polysaccharides containing high concentrations of pyruvate (22%) and uronic acids (18%). These polysaccharides may play an important role in metal complexation and protection from desiccation in A. coffeaeformis.
authors: a      Marine Corrosion and Materials Division   ,  National Institute of Oceanography   ,  Dona Paula ,  Goa ,  403004 ,    India    Fax:
authors: N B
authors: NB
authors: Bhosle
authors: S S
authors: SS
authors: Sawant
authors: A
authors: A
authors: Garg
authors: A B
authors: AB
authors: Wagh
authors: L V
authors: LV
authors: Evans