title: USPIO-enhanced MRI of highly invasive and highly metastasizing transplanted human squamous cell carcinoma: an experimental study.
abstract: OBJECTIVE
  The aim of this study was to investigate the signal intensity characteristics of highly invasive and highly metastasizing transplanted human squamous cell carcinoma using ultra-small super-paramagnetic iron oxide (USPIO)-enhanced MRI and to correlate them with USPIO distribution to tumour components revealed by histological examination.
  
  
  METHODS
  13 nude mice with transplanted human squamous cell carcinoma in the oral cavity were imaged before and 24 hours after intravenous administration of USPIO. The difference in signal intensity between pre-contrast and post-contrast MR images was visually evaluated. For quantitative analysis, signal intensity within a region of interest was measured. Histological findings were correlated with MR findings. The approximate USPIO concentration was evaluated using USPIO phantoms.
  
  
  RESULTS
  Seven tumours had an area showing signal intensity increase on post-contrast T₁ weighted images. Histopathologically, six of those tumours contained a small amount of iron particles in the stroma. The USPIO concentration was presumed low. Two tumours had an area showing signal intensity decrease on post-contrast T₁ and T₂ weighted images. The areas had a large amount of iron particles in the stroma and the USPIO concentration was presumed high. There was a minimal amount of iron particles in tumour parenchymal cells.
  
  
  CONCLUSIONS
  The amount of USPIO accumulation into tumour stroma was considered to affect MR signal intensity. A small amount increases T₁ weighted signal intensity, whereas a large amount decreases T₁ and T₂ weighted intensity. The USPIO accumulation into the tumour parenchyma was not thought to affect MR signal intensity.
authors: Oral and Maxillofacial Radiology, Graduate School, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan. akemi.orad@tmd.ac.jp
authors: A
authors: A
authors: Tetsumura
authors: S
authors: S
authors: Nakamura
authors: N
authors: N
authors: Yoshino
authors: H
authors: H
authors: Watanabe
authors: A
authors: A
authors: Kuribayashi
authors: K
authors: K
authors: Nagumo
authors: N
authors: N
authors: Okada
authors: T
authors: T
authors: Sasaki
authors: T
authors: T
authors: Kurabayashi
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging* / Q000473:pathology / Q000556:secondary; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003238:Connective Tissue / Q000000981:diagnostic imaging / Q000473:pathology; D003287:Contrast Media / Q000008:administration & dosage / Q000592:standards; D003911:Dextrans / Q000008:administration & dosage / Q000592:standards; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007275:Injections, Intravenous; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000008:administration & dosage / Q000592:standards; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation / Q000000981:diagnostic imaging* / Q000473:pathology; D019047:Phantoms, Imaging / Q000592:standards; D011859:Radiography; D012015:Reference Standards; D014062:Tongue Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10