title: Enhanced flavonoid production in Streptomyces venezuelae via metabolic engineering.
abstract: Metabolic engineering of plant-specific phenylpropanoid biosynthesis has attracted an increasing amount of attention recently, owing to the vast potential of flavonoids as nutraceuticals and pharmaceuticals. Recently, we have developed a recombinant Streptomyces venezuelae as a heterologous host for the production of flavonoids. In this study, we successfully improved flavonoid production by expressing two sets of genes predicted to be involved in malonate assimilation. The introduction of matB and matC encoding for malonyl-CoA synthetase and the putative dicarboxylate carrier protein, respectively, from Streptomyces coelicolor into the recombinant S. venezuelae strains expressing flavanone and flavone biosynthetic genes resulted in enhanced production of both flavonoids.
authors: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors: Sung Ryeol
authors: SR
authors: Park
authors: Mi Sun
authors: MS
authors: Ahn
authors: Ah Reum
authors: AR
authors: Han
authors: Je Won
authors: JW
authors: Park
authors: Yeo Joon
authors: YJ
authors: Yoon
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism; D029382:Dicarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000096:biosynthesis*; D015870:Gene Expression; D008314:Malonates / Q000378:metabolism; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029382:Dicarboxylic Acid Transporters; D005419:Flavonoids; D008314:Malonates; D003066:Coenzyme A Ligases; C021533:malonyl-CoA synthetase