title: Myosin VI in PC12 cells plays important roles in cell migration and proliferation but not in catecholamine secretion.
abstract: Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments and is believed to play distinct role(s) than other myosins. We addressed a role of this unique motor in secretory PC12 cells, derived from rat adrenal medulla pheochromocytoma using cell lines with reduced MVI synthesis (produced by means of siRNA). Decrease of MVI expression caused severe changes in cell size and morphology, and profound defects in actin cytoskeleton organization and Golgi structure. Also, significant inhibition of cell migration as well as cell proliferation was observed. Flow cytometric analysis revealed that MVI-deficient cells were arrested in G0/G1 phase of the cell cycle but did not undergo increased senescence as compared with control cells. Also, neither polyploidy nor aneuploidy were detected. Surprisingly, no significant effect on noradrenaline secretion was observed. These data indicate that in PC12 cells MVI is involved in cell migration and proliferation but is not crucial for stimulation-dependent catecholamine release.
authors: Department of Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093, Warsaw, Poland.
authors: Łukasz
authors: Ł
authors: Majewski
authors: Magdalena
authors: M
authors: Sobczak
authors: Anna
authors: A
authors: Wasik
authors: Krzysztof
authors: K
authors: Skowronek
authors: Maria Jolanta
authors: MJ
authors: Rędowicz
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002395:Catecholamines / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D048429:Cell Size; D005434:Flow Cytometry; D006056:Golgi Apparatus / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats
chemical_list: D002395:Catecholamines; C089785:myosin VI; D018995:Myosin Heavy Chains