title: Demineralized bone particle impregnated poly(l-lactide-co-glycolide) scaffold for application in tissue-engineered intervertebral discs.
abstract: Demineralized bone particle (DBP) contains powerful bioactive molecules that facilitate new bone or cartilage growth. We developed hybrid scaffolds of poly(l-lactide-co-glycolide) (PLGA) with various concentrations of DBP (DBP/PLGA), of which phenotypes on intervertebral disc (IVD) cells were investigated. The hybrid scaffold has a cylindrical donut shape with two distinct parts; the inner is for the nucleus pulposus (NP) and the outer is for annulus fibrosus (AF). Rabbit NP and AF cells were seeded into the inner and outer regions of the DBP/PLGA scaffolds separately. Disc cell viability in DBP/PLGA scaffolds was superior to pure PLGA scaffold and increased with increasing DBP concentration. In vitro- and in vivo-formed tissues were characterized by RT-PCR, Safranin-O, Masson's trichrome staining and immunohistochemi- cal staining for type-I and type-II collagen. DBP/PLGA hybrid scaffolds revealed more active expression of disc phenotypes, as characterized by protein and mRNA expression, than the PLGA control. This study provides valuable information for potential disc replacement using DBP and PLGA.
authors: a Department of BIN Fusion Technology, Department of Polymer Nano Science Technology and Polymer Fusion Research Center , Chonbuk National University , 567 Beakje-daero , Deokjin , Jeonju , 561-756 , South Korea.
authors: Soon Hee
authors: SH
authors: Kim
authors: Jeong Eun
authors: JE
authors: Song
authors: Dongwon
authors: D
authors: Lee
authors: Gilson
authors: G
authors: Khang
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique / Q000379:methods*; D018786:Bone Substitutes / Q000737:chemistry; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D005269:Femur / Q000502:physiology; D007403:Intervertebral Disc / Q000166:cytology / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011474:Prosthesis Design; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D018786:Bone Substitutes; D024042:Collagen Type I; D024043:Collagen Type II; D012333:RNA, Messenger; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid