title: Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD.
abstract: The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1h. The detection (S/N=3) and quantification (S/N=10) limits for the ginsenosides ranged 0.02-0.10 ng and 0.1-0.3 ng, respectively. The linear regression coefficients ranged 0.9975-0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08-103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.
authors: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
authors: Sa-Im
authors: SI
authors: Lee
authors: Ha-Jeong
authors: HJ
authors: Kwon
authors: Yong-Moon
authors: YM
authors: Lee
authors: Je-Hyun
authors: JH
authors: Lee
authors: Seon-Pyo
authors: SP
authors: Hong
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods* / Q000706:statistics & numerical data; D003217:Conductometry / Q000379:methods* / Q000706:statistics & numerical data; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D036145:Ginsenosides