title: [Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis].
abstract: OBJECTIVE
  To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth (SGDs) with porous calcium phosphate cement (pCPC).
  
  
  METHODS
  The expression of STRO-1 on SGDs was measured with flow cytometry (FCM); the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days. Combined with pCPC, the adhesion and growth of the compounds were observed with scanning electron microscopy (SEM); the ectopic bone-like tissue formation was observed 8 weeks after the compounds implanted subcutaneously into the nude mice.
  
  
  RESULTS
  On the third day of SGDs compounded with pCPC, SEM verified that the cells adhered closely and tightly with pCPC, protruded pseudopods and secreted matrix. 8 weeks after the compounds implanted in ectopic sites, HE staining confirmed the formation of bone-like tissue; Immunohistochemistry showed the strongly positive expression of OCN protein in the implanted materials.
  
  
  CONCLUSIONS
  SGDs may differentiate into osteoblast and are potential to induce bone matrix formation; combined with pCPC, the compounds may generate bone-like tissue.
authors: Department of Stomatology, Shanghai Jiao Tong University, Shanghai, China. zhwdzw1@126.com
authors: Wei
authors: W
authors: Zhao
authors: Yong-ming
authors: YM
authors: Hao
authors: De-rong
authors: DR
authors: Zou
authors: Xiu-li
authors: XL
authors: Zhang
authors: Jia-yu
authors: JY
authors: Lu
authors: Li
authors: L
authors: Hua
authors: Cun-hua
authors: CH
authors: Cao
mesh_terms: D000818:Animals; D002130:Calcium Phosphates; D003738:Dental Cements; D003739:Dental Cementum; D006041:Goats; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts; D010012:Osteogenesis; D013234:Stem Cells; D014094:Tooth, Deciduous
chemical_list: D002130:Calcium Phosphates; D003738:Dental Cements; C020243:calcium phosphate