title: Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
abstract: We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 × 10(-11) M E2 in a microfluidic format.
authors: Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China. zhaoshulin001@163.com
authors: Shulin
authors: S
authors: Zhao
authors: Jinwen
authors: J
authors: Liu
authors: Yong
authors: Y
authors: Huang
authors: Yi-Ming
authors: YM
authors: Liu
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D004735:Energy Transfer; D004958:Estradiol / Q000032:analysis* / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D044085:Microfluidics / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens; D005456:Fluorescent Dyes; D004958:Estradiol; D008165:Luminol; C031912:6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione; D016650:Fluorescein-5-isothiocyanate