title: Design of a multinuclear Zn(II) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins.
abstract: A Zn(II) complex (Zn(II)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(II)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.
authors: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto, 615-8510, Japan.
authors: Sho-hei
authors: SH
authors: Fujishima
authors: Hiroshi
authors: H
authors: Nonaka
authors: Sho-hei
authors: SH
authors: Uchinomiya
authors: Yoshiyuki Alex
authors: YA
authors: Kawase
authors: Akio
authors: A
authors: Ojida
authors: Itaru
authors: I
authors: Hamachi
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006639:Histidine / Q000032:analysis*; D006801:Humans; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000032:analysis*; D043562:Receptors, G-Protein-Coupled / Q000032:analysis*; D015032:Zinc / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D056831:Coordination Complexes; D005456:Fluorescent Dyes; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D043562:Receptors, G-Protein-Coupled; C085321:cyanine dye 5; D006639:Histidine; D015032:Zinc