title: siRNA-mediated silencing of the gene for heat shock transcription factor 1 causes hypersensitivity to methylmercury in HEK293 cells.
abstract: Methylmercury is a hazardous heavy metal compound that can damage the central nervous system. The mechanisms of methylmercury toxicity and the biological defense mechanisms against it remain unclear. We employed gene knockdown using siRNA to search for transcription factors involved in the manifestation of methylmercury toxicity. We found that the inhibition of expression of the gene for heat shock transcription factor 1 (HSF1) sensitized HEK293 cells to methylmercury.
authors: Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences,Tohoku University, Sendai, Miyagi, Japan.
authors: Gi-Wook
authors: GW
authors: Hwang
authors: Katsunori
authors: K
authors: Ryoke
authors: Jin-Yong
authors: JY
authors: Lee
authors: Tsutomu
authors: T
authors: Takahashi
authors: Akira
authors: A
authors: Naganuma
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity*; D020868:Gene Silencing; D057809:HEK293 Cells; D000076249:Heat Shock Transcription Factors; D006801:Humans; D008767:Methylmercury Compounds / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D004785:Environmental Pollutants; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D008767:Methylmercury Compounds; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors