title: Receptor-independent infection by mutant viruses newly isolated from the neuropathogenic mouse hepatitis virus srr7 detected through a combination of spinoculation and ultraviolet radiation.
abstract: The mouse hepatitis virus (MHV) has a high mutation rate, leading to various  neuropathologies after infection. The srr7 mutant was isolated from the MHV strain cl-2, which  induces characteristic spongiform degeneration in the brain. To investigate outcomes of srr7  infection, we re-cloned srr7(H2) from the viral stock srr7(Mix). During this re-cloning, we  obtained the mutant viruses, Mu-1, Mu-2, and Br-1 which was isolated from the mice brain infected  with srr7(Mix). We examined mutant viruses for infectivity independent of the major MHV receptor  (MHVR), because these mutants exhibited high virulence similar to cl-2, which is MHVR-independent.  To confirm MHVR-independence in vitro, we used a combination of spinoculation and ultraviolet  radiation to detect distinct plaque formation (SpinoPlaque(UV+)) afrer infection of BHK cells,  which do not express MHVR. Using this technique, we found that the unique neuropathologies caused  by infection with the mutant viruses result from infecting neurons, which do not express MHVR.  Infection with the mutant viruses was 100% correlated with SpinoPlaque(UV+) formation. This is  in contrast to infection with srr7, which does not from SpinoPlaque(UV+).
authors: Department of Bioinformatics, Soka University, Tokyo, Japan.
authors: Risa
authors: R
authors: Nomura
authors: Hiromi
authors: H
authors: Kashiwazaki
authors: Masatoshi
authors: M
authors: Kakizaki
authors: Shutoku
authors: S
authors: Matsuyama
authors: Fumihiro
authors: F
authors: Taguchi
authors: Rihito
authors: R
authors: Watanabe
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology; D002494:Central Nervous System Infections / Q000473:pathology* / Q000821:virology*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000472:pathogenicity* / Q000528:radiation effects*; D009154:Mutation; D014466:Ultraviolet Rays; D014774:Virulence