title: Microscale thermophoresis as a sensitive method to quantify protein: nucleic acid interactions in solution.
abstract: Microscale thermophoresis (MST) is a new method that enables the quantitative analysis of molecular interactions in solution at the microliter scale. The technique is based on the thermophoresis of molecules, which provides information about molecule size, charge, and hydration shell. Since at least one of these parameters is typically affected upon binding, the method can be used for the analysis of each kind of biomolecular interaction or modification of proteins or DNA. Quantitative binding parameters are obtained by using a serial dilution of the binding substrate. This section provides a detailed protocol describing the analysis of DNA-protein interactions, using the AT-hook peptides as a model system that bind to short double-stranded DNA.
authors: Biochemistry III, Universität Regensburg, Regensburg, Germany.
authors: Karina
authors: K
authors: Zillner
authors: Moran
authors: M
authors: Jerabek-Willemsen
authors: Stefan
authors: S
authors: Duhr
authors: Dieter
authors: D
authors: Braun
authors: Gernot
authors: G
authors: Längst
authors: Philipp
authors: P
authors: Baaske
mesh_terms: D015342:DNA Probes / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry / Q000379:methods; D007259:Infrared Rays; D007834:Lasers; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D055550:Protein Stability; D013816:Thermodynamics; D017186:Titrimetry
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes