title: Lymphatic vessels and high endothelial venules are increased in the sentinel lymph nodes of patients with oral squamous cell carcinoma before the arrival of tumor cells.
abstract: OBJECTIVE
  To investigate the change of vasculature in the sentinel lymph node (SLN) of patients with oral squamous cell carcinoma.
  
  
  METHODS
  Immunohistochemical staining of SLNs in 58 patients was performed with two monoclonal antibodies (MAb): anti-D2-40 MAb for lymphatic endothelial cells, and anti-MECA-79 MAb for high endothelial venules (HEV). Twelve metastatically involved (m(+)) SLNs, 120 uninvolved (m(-)) SLNs, and 35 non-SLNs (control) were available for analyses. Vessel densities were measured by computer-assisted analyses in the entire region of SLN. Correlations were assessed between vessel density and clinicopathologic variables, including vascular endothelial growth factor C of primary tumor.
  
  
  RESULTS
  Lymphatic vessel density (LVD) in SLNs was higher than that in control LNs [2361.8 μm(2)/high-power field (HPF) (624.3-4758.5) vs. 1621.9 μm(2)/HPF (465.3-3453.5), P = 0.005]. LVD of m(-) SLNs [2662.4 μm(2)/HPF (624.3-4758.5)] and m(±) SLNs [4946.6 μm(2)/HPF (2009.3-8698.8)] were both statistically significantly higher compared to control. HEV densities in m(-) SLNs [14029.7 μm(2)/HPF (10465.7-17927.1)] as well as m(±) SLNs [18258.5 μm(2)/HPF (8408.9-27706.0)] were also significantly higher than those in control [10350.5 μm(2)/HPF (7807.8-12541.1)]. By multivariate analysis, the degree of vascular endothelial growth factor C expression of primary tumor showed significant correlation with LVD of SLNs (odds ratio 9.46, 95% confidence interval 1.73-51.5, P = 0.009), which was not the case in HEV.
  
  
  CONCLUSIONS
  Lymphatic vessels and HEVs were increased in SLNs, regardless of metastatic status of SLNs. Vascular endothelial growth factor C expression of primary tumor may contribute to the premetastatic change within SLNs of oral squamous cell carcinoma.
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Republic of Korea.
authors: Man Ki
authors: MK
authors: Chung
authors: In-Gu
authors: IG
authors: Do
authors: Eunwook
authors: E
authors: Jung
authors: Young-Ik
authors: YI
authors: Son
authors: Han-Sin
authors: HS
authors: Jeong
authors: Chung-Hwan
authors: CH
authors: Baek
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000556:secondary*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000098:blood supply*; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology*; D011230:Precancerous Conditions / Q000473:pathology*; D021701:Sentinel Lymph Node Biopsy; D014699:Venules / Q000473:pathology*; D055815:Young Adult