title: Modulation of gene expression of carotene biosynthesis-related protein by photosynthetic electron transport for the acclimation of intertidal macroalga Ulva fasciata to hypersalinity and excess light.
abstract: A gene (UfCBR) encoding carotene biosynthesis-related (CBR) protein that potentially functions for the dissipation of excessive energy has been cloned from the intertidal green macroalga Ulva fasciata Delile. Hypersalinity and high light ≥300 µmol m(-2)  s(-1)  increased both UfCBR mRNA level and non-photochemical quenching (NPQ). The increase of UfCBR mRNA level and NPQ by high light was inhibited by treatment of photosynthetic electron transport inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea or 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, but not by stigmatellin, an inhibitor that blocks electron transfer from quinol oxidase to iron-sulfur protein in cytochrome b(6) f complex. Treatment of dimethylthiourea, an H(2) O(2)  scavenger, under 1200 µmol m(-2)  s(-1)  condition inhibited H(2) O(2)  accumulation but did not affect UfCBR mRNA level, while treatment of H(2) O(2)  in 150 µmol m(-2)  s(-1)  condition decreased UfCBR mRNA level. Thus, an reactive oxygen species-independent redox control via a more reduced state downstream the cytochrome b(6) f complex is involved in high light up-regulation of UfCBR expression in U. fasciata. The expression of UfCBR in U. fasciata against oxidative stress occurring in high light or high salinity in relation to NPQ is discussed.
authors: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors: Yuan-Ting
authors: YT
authors: Hsu
authors: Tse-Min
authors: TM
authors: Lee
mesh_terms: D000064:Acclimatization; D002338:Carotenoids / Q000096:biosynthesis* / Q000235:genetics; D045346:Cytochrome b6f Complex / Q000235:genetics / Q000378:metabolism; D003991:Dibromothymoquinone / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008027:Light; D010100:Oxygen / Q000378:metabolism; D055668:Photochemical Processes; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011090:Polyenes / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D044445:Ulva / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011090:Polyenes; D012333:RNA, Messenger; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D003991:Dibromothymoquinone; D002338:Carotenoids; D012965:Sodium Chloride; C038983:1,3-dimethylthiourea; D045346:Cytochrome b6f Complex; C041573:stigmatellin; D004237:Diuron; D006861:Hydrogen Peroxide; D013890:Thiourea; D010100:Oxygen