title: Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing             cancer metastasis.
abstract: miRNA globally deregulates human carcinoma. A critical open question is how many                 miRNAs functionally participate in cancer development, particularly in metastasis.                 We systematically evaluate the capability of all known human miRNAs to regulate                 certain metastasis-relevant cell behaviours. To perform the high-throughput screen                 of miRNAs, which regulate cell migration, we developed a novel self-assembled cell                 microarray. Here we show that over 20% of miRNAs have migratory regulation activity                 in diverse cell types, indicating a general involvement of miRNAs in migratory                 regulation. MiR-23b, which is downregulated in human colon cancer samples, potently                 mediates the multiple steps of metastasis, including tumour growth, invasion and                 angiogenesis in vivo. It regulates a cohort of prometastatic targets,                 including FZD7 or MAP3k1. These findings provide new insight into the                 physiological and potential therapeutic importance of miRNAs as a new class of                 functional modulators.
authors: Biomedical Engineering Department, College of Engineering, Peking University, Beijing 100871, China.
authors: Hanshuo
authors: H
authors: Zhang
authors: Yang
authors: Y
authors: Hao
authors: Junyu
authors: J
authors: Yang
authors: Ying
authors: Y
authors: Zhou
authors: Juan
authors: J
authors: Li
authors: Shenyi
authors: S
authors: Yin
authors: Changhong
authors: C
authors: Sun
authors: Ming
authors: M
authors: Ma
authors: Yanyi
authors: Y
authors: Huang
authors: Jianzhong Jeff
authors: JJ
authors: Xi
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D045325:HCT116 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology*; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: C115810:FZD7 protein, human; D051157:Frizzled Receptors; D035683:MicroRNAs; D048728:MAP Kinase Kinase Kinase 1; C482576:MAP3K1 protein, human