title: Participation of calbindin-D28K in nociception: results from calbindin-D28K knockout mice.
abstract: Since calbindin-D(28K) (CB-D(28K))-positive neurons have been related to nociceptive sensory processing, we have hypothesized that altered CB-D(28K) expression could alter nociceptive transmission. We have used +/+ and -/- knockout (KO) mice for CB-D(28k) in different behavioral models of pain and sensory responses at the caudalis subdivision of the trigeminal spinal nucleus in order to understand how this protein may participate in nociception. Behavioral responses to formalin injection in the hind paw or at the whisker pad or in the hind paw glutamate or i.p. acetic acid tests showed an increase of the pain threshold in CB-D(28k) -/- mice. KO mice showed a diminution of the inhibitory activity at Sp5C nucleus and a marked reduction of GABA content. Sp5C neurons from CB-D(28k) -/- mice did not change their spontaneous activity or tactile response after formalin injection in the whisker pad. In contrast, Sp5C neurons increased their spontaneous firing rate and tactile response after formalin injection in their receptive field in CB-D(28k) +/+ mice. The results of this study demonstrate the active role played by CB-D(28k) in nociceptive sensory transmission. The lack of this calcium binding protein, associated to deficient GABAergic neurotransmission, translates into dysfunction of sensory processing of nociceptive stimuli.
authors: Instituto Teófilo Hernando, Department of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, C/ Arzobispo Morcillo, 4, 28029, Madrid, Spain.
authors: Javier
authors: J
authors: Egea
authors: Eduardo
authors: E
authors: Malmierca
authors: Angelo O
authors: AO
authors: Rosa
authors: Laura
authors: L
authors: del Barrio
authors: Pilar
authors: P
authors: Negredo
authors: Angel
authors: A
authors: Nuñez
authors: Manuela G
authors: MG
authors: López
mesh_terms: D000009:Abdominal Muscles / Q000187:drug effects; D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D064092:Calbindin 1; D064026:Calbindins; D005260:Female; D005557:Formaldehyde / Q000009:adverse effects; D005968:Glutamate Decarboxylase / Q000096:biosynthesis; D018698:Glutamic Acid / Q000633:toxicity; D006120:Grooming / Q000187:drug effects; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000187:drug effects; D009474:Neurons / Q000502:physiology*; D059225:Nociception / Q000502:physiology*; D012130:Respiratory Hypersensitivity; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis / Q000502:physiology*; D009435:Synaptic Transmission; D014275:Trigeminal Caudal Nucleus / Q000502:physiology*; D014738:Vibrissae / Q000187:drug effects
chemical_list: C576499:Calb1 protein, mouse; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D005557:Formaldehyde; D018698:Glutamic Acid; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2; D019342:Acetic Acid