title: Ligand-induced changes in T box antiterminator RNA stability.
abstract: The T box antiterminator RNA element is an important component of the T box riboswitch that controls the transcription of vital genes in many Gram-positive bacteria. A series of 1,4-disubstituted 1,2,3-triazoles was screened in a fluorescence-monitored thermal denaturation assay to identify ligands that altered the stability of antiterminator model RNA. Several ligands were identified that significantly increased or decreased the melting temperature (T(m) ) of the RNA. The results indicate that this series of triazole ligands can alter the stability of antiterminator model RNA in a structure-dependent manner.
authors: Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA.
authors: Shu
authors: S
authors: Zhou
authors: George
authors: G
authors: Acquaah-Harrison
authors: Karen D
authors: KD
authors: Jack
authors: Stephen C
authors: SC
authors: Bergmeier
authors: Jennifer V
authors: JV
authors: Hines
mesh_terms: D020121:5' Untranslated Regions; D005456:Fluorescent Dyes / Q000737:chemistry; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D009691:Nucleic Acid Denaturation; D020871:RNA Stability; D012329:RNA, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D058928:Riboswitch; D044366:Transition Temperature / Q000187:drug effects; D014230:Triazoles / Q000494:pharmacology
chemical_list: D020121:5' Untranslated Regions; D005456:Fluorescent Dyes; D008024:Ligands; D012329:RNA, Bacterial; D058928:Riboswitch; D014230:Triazoles