title: SOS1 gene overexpression increased salt tolerance in transgenic tobacco by maintaining a higher K(+)/Na(+) ratio.
abstract: Crop productivity is greatly affected by soil salinity, so improvement in salinity tolerance of crops is a major objective of many studies. We overexpressed the Arabidopsis thaliana SOS1 gene, which encodes a plasma membrane Na(+)/H(+) antiporter, in tobacco (Nicotiana tabacum cv. Xanthi-nc). Compared with nontransgenic plants, seeds from transgenic tobacco had better germination under 120 mM (mmol L(-1)) NaCl stress; chlorophyll loss in the transgenic seedlings treated with 360 mM NaCl was less; transgenic tobacco showed superior growth after irrigation with NaCl solutions; and transgenic seedlings with 150 mM NaCl stress accumulated less Na(+) and more K(+). In addition, roots of SOS1-overexpressing seedlings lost less K(+) instantaneously in response to 50 mM NaCl than control plants. These results showed that the A. thaliana SOS1 gene potentially can improve the salt tolerance of other plant species.
authors: State Key Laboratory of Plant Physiology and Biochemistry, Department of Agronomy, Centre of Crop Chemical Control, College of Agronomy and Biotechnology, China Agricultural University, 2#, Yuanmingyuan Xilu, Haidian District, Beijing 100193, PR China.
authors: Yuesen
authors: Y
authors: Yue
authors: Mingcai
authors: M
authors: Zhang
authors: Jiachang
authors: J
authors: Zhang
authors: Liusheng
authors: L
authors: Duan
authors: Zhaohu
authors: Z
authors: Li
mesh_terms: D016960:Agrobacterium tumefaciens; D017920:Antiporters / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics; D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism*; D020837:SOS1 Protein / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D017920:Antiporters; D020837:SOS1 Protein; D012965:Sodium Chloride; D012964:Sodium; D011188:Potassium