title: Affirmative effects of iloprost on apoptosis during ischemia-reperfusion injury in kidney as a distant organ.
abstract: OBJECTIVE
  Apoptosis and its regulatory mechanisms take part in renal ischemia-reperfusion (I/R) injury which can result in acute renal failure and the inhibition of the caspase is considered as a new therapeutic strategy. In this context, we investigated the antiapoptotic and cytoprotective effects of iloprost, a prostacyclin analog, in kidney as a distant organ.
  
  
  METHODS
  Wistar albino rats were randomized into five groups (n = 12 in each) as sham, ischemia, I/R, iloprost (10 μg kg(-1)), and I/R + iloprost (10 μg kg(-1)). A 4 h reperfusion procedure was carried out after 4 h of ischemia. Caspase-8 was evaluated for death receptor-induced pathways, whereas caspase-9 was evaluated for mitochondria-dependent pathways and caspase-3 was investigated for overall apoptosis. Superoxide dismutase (SOD) enzyme activity and nitrite content as an indicator of nitric oxide (NO) production were also analyzed in kidney tissues.
  
  
  RESULTS
  Caspases-3, -8, and -9 were all significantly elevated in both ischemia and I/R groups compared to the sham group; however, treatment with iloprost reduced caspases-3, -8, and -9. SOD enzyme activity was attenuated by iloprost when compared to ischemic rats. The different effects of NO were found which change according to the present situation in ischemia, I/R, and treatment with iloprost.
  
  
  CONCLUSIONS
  These findings suggested that iloprost prevents apoptosis in both receptor-induced and mitochondria-dependent pathways in renal I/R injury and it may be considered as a cytoprotective agent for apoptosis. Understanding the efficiency of iloprost on the pathways for cell death may lead to an opportunity in the therapeutic approach for renal I/R injury.
authors: Department of Biochemistry, Faculty of Pharmacy, Mersin University, Mersin, Turkey. ncanacankatan@yahoo.com
authors: Necmiye
authors: N
authors: Canacankatan
authors: Nehir
authors: N
authors: Sucu
authors: Barlas
authors: B
authors: Aytacoglu
authors: Oguz E
authors: OE
authors: Gul
authors: Aysegul
authors: A
authors: Gorur
authors: Belma
authors: B
authors: Korkmaz
authors: Seyhan
authors: S
authors: Sahan-Firat
authors: Efsun S
authors: ES
authors: Antmen
authors: Lülüfer
authors: L
authors: Tamer
authors: Lokman
authors: L
authors: Ayaz
authors: Ozden
authors: O
authors: Vezir
authors: Arzu
authors: A
authors: Kanik
authors: Bahar
authors: B
authors: Tunctan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016285:Iloprost / Q000494:pharmacology* / Q000627:therapeutic use; D007668:Kidney / Q000098:blood supply*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology*
chemical_list: D016285:Iloprost