title: Assessing cell size and cell cycle regulation in cells with altered TOR activity.
abstract: Target of rapamycin (TOR) regulates the growth of cells and organisms. Numerous growth-promoting and growth-arresting pathways converge on TOR; TOR acts as an important hub, balancing the pro- and anti-growth signals within a cell. Since it regulates growth at the cellular level, cell size can be used as an indirect readout of TOR activity. Here, we describe methods used to analyze cell size in cell culture and in the Drosophila wing.
authors: Signal Transduction Laboratory, Cancer Research UK London Research Institute, London, UK.
authors: Megan
authors: M
authors: Cully
authors: Julian
authors: J
authors: Downward
mesh_terms: D000818:Animals; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000032:analysis; D048429:Cell Size; D002478:Cells, Cultured; D029721:Drosophila Proteins / Q000032:analysis / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000254:growth & development; D005434:Flow Cytometry / Q000379:methods*; D011494:Protein Kinases / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases; D014921:Wings, Animal / Q000166:cytology* / Q000254:growth & development
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D034741:RNA, Small Interfering; C117726:gig protein, Drosophila; D011494:Protein Kinases; C416303:target of rapamycin protein, Drosophila; D058570:TOR Serine-Threonine Kinases