title: Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds.
abstract: Plant oils can be useful chemical feedstocks such as a source of epoxy fatty acids. High seed-specific expression of a Stokesia laevis epoxygenase (SlEPX) in soybeans only results in 3-7% epoxide levels. SlEPX-transgenic soybean seeds also exhibited other phenotypic alterations, such as altered seed fatty acid profiles, reduced oil accumulation, and variable protein levels. SlEPX-transgenic seeds showed a 2-5% reduction in total oil content and protein levels of 30.9-51.4%. To address these pleiotrophic effects of SlEPX expression on other traits, transgenic soybeans were developed to co-express SlEPX and DGAT (diacylglycerol acyltransferase) genes (VgDGAT1 & 2) isolated from Vernonia galamensis, a high accumulator of epoxy fatty acids. These side effects of SlEPX expression were largely overcome in the DGAT co-expressing soybeans. Total oil and protein contents were restored to the levels in non-transgenic soybeans, indicating that both VgDGAT1 and VgDGAT2 could complement the disrupted phenotypes caused by over-expression of an epoxygenase in soybean seeds.
authors: Department of Plant and Soil Science, University of Kentucky, KY 40546-0312, USA.
authors: Runzhi
authors: R
authors: Li
authors: Keshun
authors: K
authors: Yu
authors: Yongmei
authors: Y
authors: Wu
authors: Mizuki
authors: M
authors: Tateno
authors: Tomoko
authors: T
authors: Hatanaka
authors: David F
authors: DF
authors: Hildebrand
mesh_terms: D051048:Diacylglycerol O-Acyltransferase / Q000096:biosynthesis / Q000235:genetics; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D013025:Glycine max / Q000201:enzymology / Q000378:metabolism; D035741:Vernonia / Q000201:enzymology / Q000235:genetics*
chemical_list: D010938:Plant Oils; D010940:Plant Proteins; D010088:Oxidoreductases; D051048:Diacylglycerol O-Acyltransferase