title: A novel genetically encoded fluorescent protein as a Cu(I) indicator.
abstract: We constructed the copper(I)-binding domain of Mycobacterium tuberculosis (Mtb) CDC 1551 (residues 1-162) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP), and formed a novel genetically encoded fluorescent copper(I) responsive protein (PMtb). The sensitivity and selectivity to copper(I) of the PMtb was sought. The experiments showed that the copper(I)-binding domain of the PMtb was highly sensitive and selective towards copper(I).
authors: Chemistry College, Beijing Normal University, Beijing, China. yanxi@bnu.edu.cn
authors: Xi
authors: X
authors: Yan
authors: Xiang
authors: X
authors: Li
authors: Sha-Sha
authors: SS
authors: Lv
authors: Da-Cheng
authors: DC
authors: He
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D003300:Copper / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D003300:Copper