title: Glycoprotein 340 and sialic acid in minor-gland and whole saliva of children, adolescents, and adults.
abstract: Glycoprotein 340 (gp-340) is a bacterial-binding glycoprotein found in major-gland and minor-gland saliva. Sialic acid, a common terminal structure of salivary glycoproteins, interacts with microorganisms and host ligands, as well as with free radicals. This study investigated the contents of gp-340 and sialic acid in minor-gland saliva and whole saliva of children (3 yr of age), adolescents (14 yr of age), and adults (20-25 yr of age). Labial-gland saliva and buccal-gland saliva were collected on filter paper, and unstimulated whole saliva was collected by draining into a tube. The relative amount of gp-340 and sialic acid was determined by ELISA and by enzyme-linked lectin assay (ELLA), respectively. In minor-gland saliva, no statistically significant differences in gp-340 and sialic acid were seen between the age-groups. Among adults, significantly lower amounts of gp-340 and sialic acid were seen in labial saliva compared with buccal saliva. In whole saliva, the amount of gp-340 was significantly lower among adults compared with children. No differences between genders were seen. Stable content of gp-340 and sialic acid in minor-gland saliva across the age-groups, and a higher content of gp-340 in the whole saliva of the youngest age-group (3-yr-olds) compared with the adult group, may reflect that those components are vital innate factors of immunity in children's saliva.
authors: Departments of Orthodontics Cariology Oral Biology, Faculty of Odontology, Malmö University, Malmö, Sweden. Mikael.Sonesson@mah.se
authors: Mikael
authors: M
authors: Sonesson
authors: Dan
authors: D
authors: Ericson
authors: Bertil
authors: B
authors: Kinnby
authors: Claes
authors: C
authors: Wickström
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000375:Aging / Q000378:metabolism; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000032:analysis* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D012470:Salivary Glands, Minor / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D055815:Young Adult
chemical_list: D011971:Receptors, Immunologic; D012471:Salivary Proteins and Peptides; C116303:glycoprotein 340; D019158:N-Acetylneuraminic Acid