title: Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology.
abstract: We review the use of thin filament-reconstituted muscle fibers in the study of muscle physiology. Thin filament extraction and reconstitution protocol is a powerful technique to study the role of each component of the thin filament. It is also useful for studying the properties of genetically modified molecules such as actin and tropomyosin. We also review the combination of this protocol with sinusoidal analysis, which will provide a solid technique for determining the effect of regulatory proteins on actomyosin interaction and concomitant cross-bridge kinetics. We suggest that thin filament-reconstituted muscle fibers are an ideal system for studying muscle physiology especially when gene modifications of actin or tropomyosin are involved.
authors: Laboratory for Comprehensive Bioimaging, Riken Quantitative Biology Center, OLABB, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan.
authors: Sayaka
authors: S
authors: Higuchi
authors: Yoshikazu
authors: Y
authors: Tsukasaki
authors: Norio
authors: N
authors: Fukuda
authors: Satoshi
authors: S
authors: Kurihara
authors: Hideaki
authors: H
authors: Fujita
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000502:physiology*; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000205:Actomyosin / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000502:physiology; D064211:Connectin; D003599:Cytoskeleton / Q000502:physiology*; D006801:Humans; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000502:physiology*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D054792:Muscle, Striated / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012518:Sarcomeres / Q000378:metabolism / Q000502:physiology; D014335:Tropomyosin / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D064211:Connectin; D009124:Muscle Proteins; C577285:TTN protein, human; D014335:Tropomyosin; D000205:Actomyosin; D011494:Protein Kinases; D002118:Calcium