title: Post-mortem re-cloning of a transgenic red fluorescent protein dog.
abstract: Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors: So Gun
authors: SG
authors: Hong
authors: Ok Jae
authors: OJ
authors: Koo
authors: Hyun Ju
authors: HJ
authors: Oh
authors: Jung Eun
authors: JE
authors: Park
authors: Minjung
authors: M
authors: Kim
authors: Geon-A
authors: GA
authors: Kim
authors: Eun Jung
authors: EJ
authors: Park
authors: Goo
authors: G
authors: Jang
authors: Byeong-Chun
authors: BC
authors: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000235:genetics*; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D013154:Spleen / Q000378:metabolism; D014132:Trachea / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein