title: Detection of NF-κB activity in skeletal muscle cells by electrophoretic mobility shift analysis.
abstract: An electrophoretic mobility shift assay (EMSA) is a common and invaluable technique which can be utilized to study the affinity of proteins to a specific DNA or RNA sequence. These assays are performed in vitro with protein extracts isolated from either cultured cells or isolated tissues. Here, we describe the methodology used to isolate the cytoplasmic and nuclear protein extracts from both cultured cells and tissues and utilize the nuclear protein fraction to assess NF-κB DNA-binding activity by EMSA analysis.
authors: Department of Molecular Virology, Immunology, and Medical Genetics, Human Cancer Genetics Program, Integrated Biomedical Sciences Graduate Program, The Ohio State University, Columbus, OH, USA.
authors: Jason M
authors: JM
authors: Dahlman
authors: Denis C
authors: DC
authors: Guttridge
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D007553:Isotope Labeling; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes
chemical_list: D016328:NF-kappa B; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes