title: Ribosomal route to small-molecule diversity.
abstract: The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in  Escherichia coli . These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.
authors: Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
authors: Ma Diarey B
authors: MD
authors: Tianero
authors: Mohamed S
authors: MS
authors: Donia
authors: Travis S
authors: TS
authors: Young
authors: Peter G
authors: PG
authors: Schultz
authors: Eric W
authors: EW
authors: Schmidt
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D057225:Data Mining; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D009154:Mutation; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000378:metabolism*
chemical_list: D012269:Ribosomal Proteins; D054852:Small Molecule Libraries