title: Assessing protein synthesis by platelets.
abstract: Platelets are anucleate cytoplasts that circulate in the bloodstream for approximately 9-11 days. Because they lack nuclei, platelets were considered incapable of protein synthesis. However, studies over the last decade have revealed that platelets use a variety of translational control pathways to synthesize proteins.A variety of protocols can be employed to assess protein synthesis by platelets. These protocols are scattered throughout the literature and, more often than not, lack critical details. In this chapter, we thoroughly outline methods used in our laboratory to assess protein synthesis by platelets.
authors: Program in Molecular Medicine, Department of Surgery, University of Utah, Salt Lake City, UT, USA.
authors: Hansjörg
authors: H
authors: Schwertz
authors: Jesse W
authors: JW
authors: Rowley
authors: Neal D
authors: ND
authors: Tolley
authors: Robert A
authors: RA
authors: Campbell
authors: Andrew S
authors: AS
authors: Weyrich
mesh_terms: D001681:Biological Assay; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional
chemical_list: D001798:Blood Proteins