title: Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins.
abstract: Development of a simple label-free fluorescence hybridization assay to monitor the depurination activity of toxic ribosome inactivating proteins by using a fluorescent ligand that specifically pseudo base pairs with a cytosine residue opposite an abasic site is described. This method could be potentially implemented in screening platforms for the discovery of small molecules that inhibit the activity of these toxins.
authors: Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, Pune 411021, India.
authors: Arun A
authors: AA
authors: Tanpure
authors: Pooja
authors: P
authors: Patheja
authors: Seergazhi G
authors: SG
authors: Srivatsan
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009287:Naphthyridines / Q000032:analysis; D009693:Nucleic Acid Hybridization / Q000379:methods; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D051381:Rats; D054789:Ribosome Inactivating Proteins, Type 1 / Q000378:metabolism*; D000076985:Saporins
chemical_list: C528376:2-amino-5,6,7-trimethyl-1,8-naphthyridine; D005456:Fluorescent Dyes; D009287:Naphthyridines; D010940:Plant Proteins; D011687:Purines; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; C030985:purine