title: Superparamagnetic iron oxide nanoparticles-loaded polymersome-mediated gene delivery guided by enhanced magnetic resonance signal.
abstract: Positively charged superparamagnetic iron oxide nanoparticle (SPION)-loaded polymersome was prepared in order to deliver genes to the target sites, which was monitored by magnetic resonance imaging (MRI), concomitantly. The transfection efficiency in vitro was tested by treating CT-26 colon cancer cell line with luciferase-expressing plasmids/SPION complex. MRI was also used to check the detectability of SPION in vitro and in vivo. SPION-loaded polymersome, carrying genetic materials, was delivered and then accumulated at the tumor site of the murine colon cancer xenograft model after intravenous injection, possibly through a passive targeting mechanism. Clinical MRI monitored this accumulation. This result indicates that the SPION-loaded polymersomecan be applied to MR image-guided gene therapy.
authors: Department of Biomedical Sciences, Chonnam National University Medical School, The Research Institute of Medical Science, Chonnam National University, Gwangju 501-746, South Korea.
authors: Sang Joon
authors: SJ
authors: Lee
authors: Hyun Jin
authors: HJ
authors: Lee
authors: Myeong-Ju
authors: MJ
authors: Moon
authors: Hieu
authors: H
authors: Vu-Quang
authors: Hwa-Jeong
authors: HJ
authors: Lee
authors: Muthunarayanan
authors: M
authors: Muthiah
authors: Hui-Lian
authors: HL
authors: Che
authors: Seon U
authors: SU
authors: Heo
authors: Hwan Jeong
authors: HJ
authors: Jeong
authors: Yong Yeon
authors: YY
authors: Jeong
authors: In-Kyu
authors: IK
authors: Park
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005290:Ferric Compounds / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053768:Metal Nanoparticles
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide