title: Application of nano-LC-based glycomics towards biomarker discovery.
abstract: The glycome, that is, the glycan components of a biological source, has been widely reported to change with disease states. However, mining the glycome for biomarkers is complicated by glycan structural heterogeneity. Nanoflow LC, or nano-LC, significantly addresses the problem by providing a highly sensitive and quantitative method of separating and profiling glycans. This review summarizes recent advances in analytical technology and methodology that enhance and augment the advantages offered by nano-LC. (e.g., reversed phase, hydrophilic interaction and porous graphitized carbon chromatography, as well as associated derivatization strategies), detectors (e.g., fluorescence and MS), and technology platforms (particularly chip-based nano-LC) are examined in detail, along with their application to biomarker discovery. Particular emphasis is placed on methods and technologies that allow structure-specific glycan profiling.
authors: Department of Chemistry, University of California, Davis, CA 95616, USA.
authors: Serenus
authors: S
authors: Hua
authors: Carlito
authors: C
authors: Lebrilla
authors: Hyun Joo
authors: HJ
authors: An
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D011134:Polysaccharides