title: Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.
abstract: We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation profiles and differentiation potential. Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin.
authors: Stem Cell Transplantation Program, Division of Pediatric Hematology/Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
authors: Kitai
authors: K
authors: Kim
authors: Rui
authors: R
authors: Zhao
authors: Akiko
authors: A
authors: Doi
authors: Kitwa
authors: K
authors: Ng
authors: Juli
authors: J
authors: Unternaehrer
authors: Patrick
authors: P
authors: Cahan
authors: Hongguang
authors: H
authors: Huo
authors: Yuin-Han
authors: YH
authors: Loh
authors: Martin J
authors: MJ
authors: Aryee
authors: M William
authors: MW
authors: Lensch
authors: Hu
authors: H
authors: Li
authors: James J
authors: JJ
authors: Collins
authors: Andrew P
authors: AP
authors: Feinberg
authors: George Q
authors: GQ
authors: Daley
mesh_terms: D002454:Cell Differentiation; D019070:Cell Lineage; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D015894:Genome, Human; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D046228:Microarray Analysis