title: Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5.
abstract: Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced host cell apoptosis has been established, the underlying molecular mechanism is still unclear. We report here that IBDV viral protein 5 (VP5) is a major apoptosis inducer in DF-1 cells by interacting with the voltage-dependent anion channel 2 (VDAC2) in the mitochondrion. We found that in DF-1 cells, VP5-induced apoptosis can be completely abolished by 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS), an inhibitor of VDAC. Furthermore, knockdown of VDAC2 by small interfering RNA markedly inhibits IBDV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome c release, leading to increased IBDV growth in host cells. Thus, VP5-induced apoptosis during IBDV infection is mediated by interacting with VDAC2, a protein that appears to restrict viral replication via induction of cell death.
authors: State Key Laboratory of Agrobiotechnology, Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, and College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors: Zhonghua
authors: Z
authors: Li
authors: Yongqiang
authors: Y
authors: Wang
authors: Yanfei
authors: Y
authors: Xue
authors: Xiaoqi
authors: X
authors: Li
authors: Hong
authors: H
authors: Cao
authors: Shijun J
authors: SJ
authors: Zheng
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D055785:Gene Knockdown Techniques; D006801:Humans; D007243:Infectious bursal disease virus / Q000502:physiology*; D008969:Molecular Sequence Data; D011485:Protein Binding; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication; D050996:Voltage-Dependent Anion Channel 2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D017931:DNA Primers; C518635:VP5 protein, infectious bursal disease virus; D017361:Viral Nonstructural Proteins; D050996:Voltage-Dependent Anion Channel 2