title: Characterization of arsenate transformation and identification of arsenate reductase in a green alga Chlamydomonas reinhardtii.
abstract: Arsenic (As) is a pervasive and ubiquitous environmental toxin that has created catastrophic human health problems world-wide. Chlamydomonas reinhardtii is a unicellular green alga, which exists ubiquitously in freshwater aquatic systems. Arsenic metabolism processes of this alga through arsenate reduction and sequent store and efflux were investigated. When supplied with 10 micromol/L arsenate, arsenic speciation analysis showed that arsenite concentration increased from 5.7 to 15.7 mg/kg dry weight during a 7-day period, accounting for 18%-24% of the total As in alga. When treated with different levels of arsenate (10, 20, 30, 40, 50 micromol/L) for 7 days, the arsenite concentration increased with increasing external arsenate concentrations, the proportion of arsenite was up to 23%-28% of the total As in alga. In efflux experiments, both arsenate and arsenite could be found in the efflux solutions. Additionally, the efflux of arsenate was more than that of arsenite. Furthermore, two arsenate reductase genes of C. reinhardtii (CrACR2s) were cloned and expressed in Escherichia coli strain WC3110 (deltaarsC) for the first time. The abilities of both CrACR2s genes to complement the arsenate-sensitive strain were examined. CrACR2.1 restored arsenate resistance at 0.8 mmol/L. However, CrACR2.2 showed much less ability to complement. The gene products were demonstrated to reduce arsenate to arsenite in vivo. In agreement with the complementation results, CrACR2.1 showed higher reduction ability than CrACR2.2, when treated with 0.4 mmol/L arsenate for 16 hr incubation.
authors: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. xxyin@iue.ac.cn
authors: Xixiang
authors: X
authors: Yin
authors: Lihong
authors: L
authors: Wang
authors: Guilan
authors: G
authors: Duan
authors: Guoxin
authors: G
authors: Sun
mesh_terms: D053502:Arsenate Reductases / Q000378:metabolism*; D001149:Arsenates / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D001149:Arsenates; D053502:Arsenate Reductases; C025657:arsenic acid