title: Low prevalence of human papillomavirus (HPV) in Chinese patients with breast cancer.
abstract: This retrospective study investigated the presence of human papillomavirus (HPV) in Chinese women with breast cancer, and the correlation between HPV infection and carcinogenesis. Tumour and non-cancerous breast tissue samples were obtained from 62 female patients with breast cancer;  normal breast tissue samples were obtained from 46 women without breast cancer. HPV DNA was detected by nested polymerase chain reaction using consensus primers; HPV subtypes were determined by reverse dot blot and pyrosequencing analyses. HPV was found in tumour tissue samples from four of  the 62 patients (6.5%), while no HPV DNA was detected in either the non-cancerous samples from patients with breast cancer or from the normal breast tissue controls. Of the four HPV-positive cases, three were HPV 16 positive (75%) and one was HPV 18 positive (25%). The low frequency of HPV  detected in this study suggests that this infection is not a major risk factor in breast cancer development.
authors: State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: X
authors: X
authors: Mou
authors: L
authors: L
authors: Chen
authors: F
authors: F
authors: Liu
authors: Y
authors: Y
authors: Shen
authors: H
authors: H
authors: Wang
authors: Y
authors: Y
authors: Li
authors: L
authors: L
authors: Yuan
authors: J
authors: J
authors: Lin
authors: J
authors: J
authors: Lin
authors: L
authors: L
authors: Teng
authors: C
authors: C
authors: Xiang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus; D001943:Breast Neoplasms / Q000821:virology*; D016022:Case-Control Studies; D002681:China; D005260:Female; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications*