title: Effects of porcine circovirus type 2 on expression of mRNA associated with endogenous antigen processing and presentation in pulmonary alveolar macrophages and circulating T lymphocytes in piglets.
abstract: Pulmonary alveolar macrophages (PAMs) are the major target cells of porcine circovirus type 2 (PCV2). This study examined cellular immunity and expression of molecules associated with endogenous antigen processing and presentation in porcine PAMs for 28 days following infection with PCV2. The numbers of total T cells and T lymphocyte subpopulations were measured by flow cytometry (FCM). The expression of mRNA of large multifunctional peptidase 7 (LMP7), ubiquitin-specific protease (UBP), heat shock proteins 70 and 90 (HSP70 and HSP90), major histocompatibility class I (MHC-I), β(2)-microglobulin, glucose-regulated protein 94 (GRP94), calnexin and calreticulin of PAMs from PCV2 infected and control pigs were determined by real-time reverse transcriptase PCR. The absolute numbers of total T cells, T helper (Th) cells, cytotoxic T cells and γδ T cells, but not memory/activated Th cells, decreased following PCV2 infection. There was decreased expression of LMP7 mRNA at 3days postinfection (DPI), whereas expression of UBP and calreticulin mRNA was increased at 3 DPI, expression of HSP90 and β(2)-microglobulin mRNA was increased at 3 and 7 DPI and expression of GRP94 mRNA was increased at 14 DPI. PAMs from PCV2-infected piglets had lower surface expression of CD80/CD86 at 7 and 14 DPI and MHC-II at 7 DPI. These findings suggest that there are alterations in cellular immune function and in the endogenous antigen presentation capacity of PAMs in PCV2-infected piglets.
authors: College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing 102206, China.
authors: Jiandong
authors: J
authors: Li
authors: Qi
authors: Q
authors: Yu
authors: Xiaohua
authors: X
authors: Nie
authors: Xinfeng
authors: X
authors: Guo
authors: Qinye
authors: Q
authors: Song
authors: Huanrong
authors: H
authors: Li
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D018173:Circoviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018655:Lymphocyte Count / Q000662:veterinary; D016676:Macrophages, Alveolar / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology* / Q000821:virology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D019562:Viral Load / Q000662:veterinary
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral