title: Primary human osteoblast cultures.
abstract: Osteoblast cultures can be used to investigate the mechanisms of bone formation, to probe the cellular and molecular basis of bone disease, and to screen for potential therapeutic agents that affect bone formation. Here, we describe the methods for establishing and characterising primary human osteoblast cultures.
authors: Bone and Joint Research Group, Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK.
authors: Jane P
authors: JP
authors: Dillon
authors: Victoria J
authors: VJ
authors: Waring-Green
authors: Adam M
authors: AM
authors: Taylor
authors: Peter J M
authors: PJ
authors: Wilson
authors: Mark
authors: M
authors: Birch
authors: Alison
authors: A
authors: Gartland
authors: James A
authors: JA
authors: Gallagher
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis; D012516:Osteosarcoma / Q000378:metabolism