title: Immunohistochemical analysis of mTOR activity in tissues.
abstract: mTOR is a key regulator of cell growth and size, and its activity is often dysregulated in a wide variety of diseases. The mTOR signaling pathway is also a therapeutic target for many diseases, including cancer. Immunohistochemistry is a powerful method to assess mTOR activity in clinical/histological samples, however, care should be taken in choosing the targets for determining mTOR activity due to the complexity of its regulation. This chapter describes the most up-to-date methods for visualizing mTOR activity by immunohistochemistry using commercially available antibodies, including considerations for validating new antibodies for assessing mTOR signaling.
authors: Department of Molecular Carcinogenesis, The University of Texas M. D. Anderson Cancer Center, Smithville, TX, USA.
authors: Jinhee
authors: J
authors: Kim
authors: Nancy
authors: N
authors: Otto
authors: Claudio J
authors: CJ
authors: Conti
authors: Irma B
authors: IB
authors: Gimenz-Conti
authors: Cheryl L
authors: CL
authors: Walker
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D053858:Metabolic Networks and Pathways; D058570:TOR Serine-Threonine Kinases / Q000032:analysis*; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D058570:TOR Serine-Threonine Kinases