title: Proteomic analysis of rat plasma following transient focal cerebral ischemia.
abstract: OBJECTIVE
  This study aimed to identify plasma protein changes in a rat model of ischemic stroke using a proteomic approach.
  
  
  METHODS
  Four male Sprague-Dawley rats (3-6 months old) were subjected to 90 min of left middle cerebral artery occlusion under anesthesia with 1.5% isoflurane in O(2)/air followed by 24-h reperfusion. Blood samples (~100 µl) were collected at baseline, at the end of 90-min middle cerebral artery occlusion and at 24-h postreperfusion. Brain injuries were assessed by MRI at 24-h postreperfusion. Quantitative comparison of global plasma protein expression was performed using 2D differential in-gel electrophoresis. Differentially expressed protein spots were identified using peptide sequencing tandem mass spectrometry.
  
  
  RESULTS
  These rats had clear brain infarction in the left hemisphere detected by MRI. Thirty-three protein spots of plasma samples were differentially expressed following focal cerebral ischemia/reperfusion. These protein spots belonged to eight proteins. Six of them (α2-macroglobulin, complement C3, inter-α- trypsin inhibitor heavy chain H3, serum albumin, haptoglobin and transthyretin), which are a class of acute-phase proteins, changed significantly.
  
  
  CONCLUSIONS
  This study describes the responses of young rats to focal cerebral ischemia and suggests that future studies should use aged animals to better mimic the clinical ischemic stroke setting.
authors: National Institute for Biological Standards & Control, Health Protection Agency, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, UK. ruoli.chen@ndm.ox.ac.uk
authors: Ruoli
authors: R
authors: Chen
authors: Iolanda
authors: I
authors: Vendrell
authors: Carl P C
authors: CP
authors: Chen
authors: Diana
authors: D
authors: Cash
authors: Kim Galley
authors: KG
authors: O'Toole
authors: Steve A
authors: SA
authors: Williams
authors: Christopher
authors: C
authors: Jones
authors: Jane E
authors: JE
authors: Preston
authors: Jun X
authors: JX
authors: Wheeler
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes / Q000737:chemistry; D002546:Ischemic Attack, Transient / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D000209:Acute-Phase Proteins; D005456:Fluorescent Dyes