title: Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells.
abstract: Frutalin is the α-D-galactose-binding lectin isolated from breadfruit seeds. Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified from Pichia pastoris. This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis. Furthermore, the interaction between frutalin and the HeLa cells was investigated by confocal microscopy. Despite having different carbohydrate-binding affinities, native and recombinant frutalin showed an identical magnitude of cytotoxicity on HeLa cells growth (IC₅₀~100 μg/mL) and equally induced cell apoptosis. The interaction studies showed that both lectins were rapidly internalised and targeted to HeLa cell's nucleus. Altogether, these results indicate that frutalin action is not dependent on its sugar-binding properties. This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity.
authors: Institute for Biotechnology and Bioengineering-IBB, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors: Carla
authors: C
authors: Oliveira
authors: Ana
authors: A
authors: Nicolau
authors: José A
authors: JA
authors: Teixeira
authors: Lucília
authors: L
authors: Domingues
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D037161:Galectins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D010843:Pichia / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D037161:Galectins; D011994:Recombinant Proteins; C570811:frutalin