title: Significance of uncoupling protein 3 in mitochondrial function upon mid- and long-term dietary high-fat exposure.
abstract: Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production after short-term (8 weeks) high-fat feeding. After 26 weeks of high-fat feeding, UCP3 knockout mice exhibited reduced mitochondrial function as measured ex vivo in isolated mitochondria. In conclusion, these data suggest that UCP3 may have a role in the protection of mitochondria against lipid-induced mitochondrial dysfunction, but only after long-term exposure to high-fat.
authors: Departments of Human Biology, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center+, Maastricht, The Netherlands.
authors: Miranda
authors: M
authors: Nabben
authors: Joris
authors: J
authors: Hoeks
authors: Esther
authors: E
authors: Moonen-Kornips
authors: Denis
authors: D
authors: van Beurden
authors: Jacob J
authors: JJ
authors: Briedé
authors: Matthijs K C
authors: MK
authors: Hesselink
authors: Jan F C
authors: JF
authors: Glatz
authors: Patrick
authors: P
authors: Schrauwen
mesh_terms: D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D007473:Ion Channels / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D017382:Reactive Oxygen Species; C000604392:UCP3 protein, human; C000604393:Ucp3 protein, mouse; D000071247:Uncoupling Protein 3