title: Combination of integrin-binding peptide and growth factor promotes cell adhesion on electron-beam-fabricated patterns.
abstract: Understanding and controlling cell adhesion on engineered scaffolds is important in biomaterials and tissue engineering. In this report we used an electron-beam (e-beam) lithography technique to fabricate patterns of a cell adhesive integrin ligand combined with a growth factor. Specifically, micron-sized poly(ethylene glycol) (PEG) hydrogels with aminooxy- and styrene sulfonate-functional groups were fabricated. Cell adhesion moieties were introduced using a ketone-functionalized arginine-glycine-aspartic acid (RGD) peptide to modify the O-hydroxylamines by oxime bond formation. Basic fibroblast growth factor (bFGF) was immobilized by electrostatic interaction with the sulfonate groups. Human umbilical vein endothelial cells (HUVECs) formed focal adhesion complexes on RGD- and RGD and bFGF-immobilized patterns as shown by immunostaining of vinculin and actin. In the presence of both bFGF and RGD, cell areas were larger. The data demonstrate confinement of cellular focal adhesions to chemically and physically well-controlled microenvironments created by a combination of e-beam lithography and "click" chemistry techniques. The results also suggest positive implications for addition of growth factors into adhesive patterns for cell-material interactions.
authors: Department of Chemistry and Biochemistry and the California NanoSystems Institute, University of California, Los Angeles, 607 Charles E. Young Drive South, Los Angeles, California 90095, USA.
authors: Christopher M
authors: CM
authors: Kolodziej
authors: Sung Hye
authors: SH
authors: Kim
authors: Rebecca M
authors: RM
authors: Broyer
authors: Sina S
authors: SS
authors: Saxer
authors: Caitlin G
authors: CG
authors: Decker
authors: Heather D
authors: HD
authors: Maynard
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004583:Electrons; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D012825:Silicon / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D016023:Integrins; D008024:Ligands; D008689:Methacrylates; D009842:Oligopeptides; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D005346:Fibroblast Growth Factors; C047981:arginyl-glycyl-aspartic acid; D012825:Silicon