title: Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells.
abstract: Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this in vitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34 μg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.
authors: Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Av. Libertad 5470, CP 3400, Corrientes, Argentina.
authors: Soledad
authors: S
authors: Bustillo
authors: Claudia C
authors: CC
authors: Gay
authors: María E
authors: ME
authors: García Denegri
authors: Luis A
authors: LA
authors: Ponce-Soto
authors: Elisa
authors: E
authors: Bal de Kier Joffé
authors: Ofelia
authors: O
authors: Acosta
authors: Laura C
authors: LC
authors: Leiva
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood; D017837:Bothrops / Q000378:metabolism*; D002460:Cell Line; D003435:Crotalid Venoms / Q000201:enzymology* / Q000494:pharmacology*; D004357:Drug Synergism; D045726:Metalloproteases / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D018482:Muscle, Skeletal / Q000187:drug effects; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology; D054467:Phospholipases A2 / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D054467:Phospholipases A2; D045726:Metalloproteases