title: Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems.
abstract: A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins.
authors: Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
authors: Karolina
authors: K
authors: Corin
authors: Philipp
authors: P
authors: Baaske
authors: Deepali B
authors: DB
authors: Ravel
authors: Junyao
authors: J
authors: Song
authors: Emily
authors: E
authors: Brown
authors: Xiaoqiang
authors: X
authors: Wang
authors: Christoph J
authors: CJ
authors: Wienken
authors: Moran
authors: M
authors: Jerabek-Willemsen
authors: Stefan
authors: S
authors: Duhr
authors: Yuan
authors: Y
authors: Luo
authors: Dieter
authors: D
authors: Braun
authors: Shuguang
authors: S
authors: Zhang
mesh_terms: D000818:Animals; D002474:Cell-Free System; D002942:Circular Dichroism; D003902:Detergents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D017433:Protein Structure, Secondary; D018035:Receptors, Odorant / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016622:Silver Staining; D012995:Solubility; D013696:Temperature
chemical_list: D003902:Detergents; D008024:Ligands; D008055:Lipids; D010455:Peptides; D018035:Receptors, Odorant; D011092:Polyethylene Glycols; C515901:Brij 35