title: Methods for evaluating the Caenorhabditis elegans dauer state: standard dauer-formation assay using synthetic daumones and proteomic analysis of O-GlcNAc modifications.
abstract: The dauer state is a non-feeding, alternative L3 state characterized by a number of distinctive metabolic and morphological changes. There are many naturally occurring dauer-inducing pheromones, termed daumones, that have been suggested by some to exhibit differences in dauer-inducing activity. Here, we have established a standard dauer-formation assay that uses synthetic daumones 1, 2, and 3, the three major daumones. To analyze the proteome of Caenorhabditis elegans in the dauer state, we focused on O-GlcNAc modification, a cytosolic modification of proteins that is known to interact either competitively or synergistically with protein phosphorylation. Protein O-GlcNAc modification is an important biological process in cells that can ensure the timely response to extracellular stimuli, such as daumone, and maintain cellular homeostasis. Establishing a standard method for assaying dauer formation using different synthetic daumones, and using differences in O-GlcNAcylated proteins during the dauer state to analyze the dauer proteome will lead to a better understanding of dauer biology of C. elegans in the context of animal longevity and adaptation under harsh environments.
authors: Yonsei Proteome Research Center, Department of Biochemistry and Biomedical Science, College of Life Science and Biotechnology, World Class University Program, Graduate School, Yonsei University, Seoul, Korea.
authors: Jeeyong
authors: J
authors: Lee
authors: Kwang-Youl
authors: KY
authors: Kim
authors: Hyoe-Jin
authors: HJ
authors: Joo
authors: Heekyeong
authors: H
authors: Kim
authors: Pan-Young
authors: PY
authors: Jeong
authors: Young-Ki
authors: YK
authors: Paik
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism*; D046508:Culture Techniques; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D006031:Glycosylation; D010675:Pheromones / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D029742:Caenorhabditis elegans Proteins; D005227:Fatty Acids; D010675:Pheromones; C496586:daumone, C elegans