title: [Effects of baicalin against oxidative stress injury of SH-SY5Y cells by up-regulating SIRT1].
abstract: This study is to investigate the effect of baicalin (BL) against oxidative injury stress of SH-SY5Y cells induced by H2O2 and the possible mechanism. SH-SY5Y cells were pre-incubated with baicalin (25, 50, and 100 micromol x L(-1)) for 12 h prior to exposure to H2O2 (150 micromol x L(-1)) for 24 h. The viability of SH-SY5Y cells was measured by MTT assay. The contents of LDH and NO were determined. The percentage of apoptotic cells was assessed by flow cytometry (FCM). The content of Caspase-3 was tested by immunofluorescence histochemical method. BL at 50 and 100 micromol x L(-1) separately increased the cell viability and up-regulated SIRT1, reduced the contents of LDH, NO, Caspase-3 and the apoptotic percentage of SH-SY5Y cells. This study results suggest that baicalin could inhibit the H2O2-induced neuronal apoptosis. The further mechanism studies show that baicalin inhibit apoptosis via reducing Caspase-3 expression and up-regulating SIRT1.
authors: Institute of Geriatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors: Hong-yan
authors: HY
authors: Chen
authors: Miao
authors: M
authors: Geng
authors: Ya-zhuo
authors: YZ
authors: Hu
authors: Jian-hua
authors: JH
authors: Wang
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D031363:Scutellaria / Q000737:chemistry; D056564:Sirtuin 1 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D009569:Nitric Oxide; C038044:baicalin; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; C505407:CASP3 protein, human; D053148:Caspase 3; C447939:SIRT1 protein, human; D056564:Sirtuin 1