title: Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (-) in a stirred-tank fermenter.
abstract: The dissolved oxygen tension of 20% of air saturation, pH-shift from 4.0 to 5.5 on day 3, and a moderate shear stress (calculated as an impeller tip speed, V(tip) = 0.926 - 2.161 m/s) were identified to be the key factors in scaling-up the mated fermentation of Blakeslea trispora NRRL 2895 (+) and 2896 (-) for lycopene production from a shake flask to a stirred-tank fermenter. The maximal lycopene production of 183.3 mg/L was obtained in 7.5-L stirred-tank fermenter, and then the mated fermentation process was successfully step-wise scaled-up from 7.5- to 200-L stirred-tank fermenter. The comparability of the fermentation process was well controlled and the lycopene production was maintained during the process scale-up. Furthermore, with the integrated addition of 150 μmol/L abscisic acid on day 3, 0.5 g/L leucine and 0.1 g/L penicillin on day 4, the highest lycopene production of 270.3 mg/L was achieved in the mated fermentation of B. trispora in stirred-tank fermenter.
authors: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, China.
authors: Xiu-Ji
authors: XJ
authors: Liu
authors: Rui-Sang
authors: RS
authors: Liu
authors: Hong-Mei
authors: HM
authors: Li
authors: Ya-Jie
authors: YJ
authors: Tang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D000077276:Lycopene; D009090:Mucorales / Q000254:growth & development*
chemical_list: D003470:Culture Media; D002338:Carotenoids; D000040:Abscisic Acid; D007930:Leucine; D000077276:Lycopene