title: Genetic dissection of axon regeneration via in vivo electroporation of adult             mouse sensory neurons.
abstract: Manipulating gene expression in vivo specifically in neurons with precise                 spatiotemporal control is important to study the function of genes or pathways in                 the nervous system. Although various transgenic approaches or virus-mediated                 transfection methods are available, they are time consuming and/or lack precise                 temporal control. Here we introduce an efficient electroporation approach to                 transfect adult dorsal root ganglion (DRG) neurons in vivo that enables                 manipulation of gene expression in an acute and precise manner. We have applied this                 method to manipulate gene expression in three widely used in vivo models of                 axon injury and regeneration, including dorsal column transection, dorsal root                 rhizotomy and peripheral axotomy. By electroporating DRGs with small interfering                 RNAs against c-jun to specifically deplete c-Jun in adult neurons, we provide                 evidence for the role of c-Jun in regulation of in vivo axon regeneration.                 This method will serve as a powerful tool to genetically dissect axon regeneration                     in vivo.
authors: Department of Orthopaedic Surgery, Johns Hopkins University                     School of Medicine, Baltimore, Maryland                 21287, USA.
authors: Saijilafu
authors: Eun-Mi
authors: EM
authors: Hur
authors: Feng-Quan
authors: FQ
authors: Zhou
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D015153:Blotting, Western; D002478:Cells, Cultured; D018274:Electroporation; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D051379:Mice; D009416:Nerve Regeneration / Q000235:genetics / Q000502:physiology*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D034741:RNA, Small Interfering