title: Osteoclast formation in mouse co-cultures.
abstract: The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D(3) and prostaglandin E(2). This assay is used particularly to analyse osteoblast-osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.
authors: Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.
authors: Cecile
authors: C
authors: Itzstein
authors: Robert J
authors: RJ
authors: van 't Hof
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002117:Calcitriol / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D015232:Dinoprostone / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002117:Calcitriol; D015232:Dinoprostone