title: A newly high alkaline lipase: an ideal choice for application in detergent formulations.
abstract: BACKGROUND
  Bacterial lipases received much attention for their substrate specificity and their ability to function in extreme environments (pH, temperature...). Many staphylococci produced lipases which were released into the culture medium. Reports of thermostable lipases from Staphylococcus sp. and active in alkaline conditions are not previously described.
  
  
  RESULTS
  A newly soil-isolated Staphylococcus sp. strain ESW secretes an induced lipase in the culture medium. The effects of temperature, pH and various components in a detergent on the activity and stability of Staphylococcus sp. lipase (SL1) were studied in a preliminary evaluation for use in detergent formulation solutions. The enzyme was highly active over a wide range of pH from 9.0 to 13.0, with an optimum at pH 12.0. The relative activity at pH 13.0 was about 60% of that obtained at pH 12.0. It exhibited maximal activity at 60°C. This novel lipase, showed extreme stability towards non-ionic and anionic surfactants after pre-incubation for 1 h at 40°C, and relative stability towards oxidizing agents. Additionally, the crude enzyme showed excellent stability and compatibility with various commercial solid and liquid detergents.
  
  
  CONCLUSIONS
  These properties added to the high activity in high alkaline pH make this novel lipase an ideal choice for application in detergent formulations.
authors: Laboratoire des Bioprocédés Environnementaux, Pôle d'Excellence Régional (PER, AUF), Centre de Biotechnologie de Sfax, Université de Sfax, B,P, "1177", Sfax 3018, Tunisia. slimcherif_enis@yahoo.fr
authors: Slim
authors: S
authors: Cherif
authors: Sami
authors: S
authors: Mnif
authors: Fatma
authors: F
authors: Hadrich
authors: Slim
authors: S
authors: Abdelkafi
authors: Sami
authors: S
authors: Sayadi
mesh_terms: D002118:Calcium / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D057075:Enzyme Assays; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D016877:Oxidants / Q000737:chemistry; D012988:Soil Microbiology; D013210:Staphylococcus / Q000201:enzymology* / Q000302:isolation & purification; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry
chemical_list: D003902:Detergents; D016877:Oxidants; D013501:Surface-Active Agents; D014280:Triglycerides; D008049:Lipase; C031627:tripropionin; D002118:Calcium