title: 2-Hydroxy-3-methylanthraquinone from Hedyotis diffusa WILLD induces apoptosis via alteration of Fas/FasL and activation of caspase-8 in human leukemic THP-1 cells.
abstract: OBJECTIVE
  Numerous studies have shown that Hedyotis diffusa WILLD (H. diffusa) could promote apoptosis in several cancer cell lines. However, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood. The current study determines the role of Fas/FasL in THP-1 cell apoptosis induced by 2-hydroxy-3-methylanthraquinone from H. diffusa.
  
  
  METHODS
  THP-1 cells were treated with 2-hydroxy-3-methylanthraquinone from H. diffusa. The effect on the cell viability of THP-1 cells was evaluated using the trypan blue assay, and cell apoptosis was measured by Giemsa staining and flow cytometry. Protein expression was assayed using the Western blot method.
  
  
  RESULTS
  Our results showed that 2-hydroxy-3-methylanthraquinone from H. diffusa induced THP-1 cell apoptosis in a time- and dose-dependent manner. Apoptosis was associated with a more prominent induction expression of Fas/FasL, DR4 and TRAIL in a time-dependent manner. Moreover, treatment of THP-1 cells with 2-hydroxy-3-methylanthraquinone from H. diffusa resulted in activation of caspase-8.
  
  
  CONCLUSIONS
  For the first time, our study suggests that 2-hydroxy-3-methylanthraquinone from H. diffusa is able to enhance apoptosis of THP-1 cells, at least in part, through activation of Fas/FasL, DR4 and TRAIL. Moreover, triggering of caspase-8 activation mediated apoptotic induction.
authors: Department of Geriatrics, Shengjing Hospital of China Medical University Shenyang, PR China. wangjh1@sj-hospital.org
authors: Jia-He
authors: JH
authors: Wang
authors: Lin-Hua
authors: LH
authors: Shu
authors: Li-Li
authors: LL
authors: Yang
authors: Meng
authors: M
authors: Zhang
authors: Ping
authors: P
authors: He
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053181:Caspase 8 / Q000378:metabolism*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D053222:Fas Ligand Protein / Q000378:metabolism*; D005434:Flow Cytometry; D032064:Hedyotis / Q000737:chemistry*; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000473:pathology*; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C571792:2-hydroxy-3-methylanthraquinone; D000880:Anthraquinones; D053222:Fas Ligand Protein; D019014:fas Receptor; D053181:Caspase 8