title: Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: seroprevalence and genotypes.
abstract: Data on the genotypes of Toxoplasma gondii circulating in wildlife are scarce. In the present study, foxes and rodents from two Federal States in Central or Eastern Germany were examined for T. gondii infections. Body fluids were collected at necropsy or fluids were obtained from frozen tissues of naturally exposed red foxes (Vulpes vulpes), voles (Microtus arvalis), shrews (Neomys anomalus) and a striped field mouse (Apodemus agrarius) and tested for T. gondii by serology. DNA isolated from tissues of seropositive foxes and all the rodents was examined by PCR. In the German Federal States of Brandenburg and Saxony-Anhalt 152/204 (74.5%) and 149/176 (84.7%) of foxes, respectively, but none of the rodents (0/72) had antibodies to T. gondii. Only 28/152 (18.4%) and 20/149 (13.4%) of seropositive foxes from Brandenburg and Saxony-Anhalt, respectively, but none of the rodents tested PCR-positive for T. gondii. The complete T. gondii genotype could be determined for twelve samples using nine PCR-restriction fragment length polymorphism (PCR-RFLP) markers (newSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico). In addition to T. gondii clonal type II (Apico II) and type II (Apico I), type III and T. gondii genotypes showing non-canonical allele patterns were observed in foxes. This suggests that, while T. gondii type II prevails in foxes, other genotypes circulate in wildlife. The population structure of T. gondii in Germany may be more diverse than previously thought.
authors: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Seestrasse 55, 16868 Wusterhausen, Germany. daland.herrmann@fli.bund.de
authors: D C
authors: DC
authors: Herrmann
authors: P
authors: P
authors: Maksimov
authors: A
authors: A
authors: Maksimov
authors: A
authors: A
authors: Sutor
authors: S
authors: S
authors: Schwarz
authors: W
authors: W
authors: Jaschke
authors: A
authors: A
authors: Schliephake
authors: N
authors: N
authors: Denzin
authors: F J
authors: FJ
authors: Conraths
authors: G
authors: G
authors: Schares
mesh_terms: D000818:Animals; D003411:Arvicolinae; D005260:Female; D005589:Foxes; D005858:Germany / Q000453:epidemiology; D008297:Male; D051189:Murinae; D012376:Rodent Diseases / Q000453:epidemiology / Q000469:parasitology; D012377:Rodentia; D016036:Seroepidemiologic Studies; D012788:Shrews; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000453:epidemiology*