title: Microcoil-based MR phase imaging and manganese enhanced microscopy of glial tumor neurospheres with direct optical correlation.
abstract: Susceptibility differences among tissues were recently used for highlighting complementary contrast in MRI different from the conventional T(1), T(2), or spin density contrasts. This method, based on the signal phase, previously showed improved image contrast of human or rodent neuroarchitecture in vivo, although direct MR phase imaging of cellular architecture was not available until recently. In this study, we present for the first time the ability of microcoil-based phase MRI to resolve the structure of human glioma neurospheres at significantly improved resolutions (10 × 10 μm(2)) with direct optical image correlation. The manganese chloride property to function as a T(1) contrast agent enabled a closer examination of cell physiology with MRI. Specifically the temporal changes of manganese chloride uptake, retention and release time within and from individual clusters were assessed. The optimal manganese chloride concentration for improved MR signal enhancement was determined while keeping the cellular viability unaffected. The presented results demonstrate the possibilities to reveal structural and functional observation of living glioblastoma human-derived cells. This was achieved through the combination of highly sensitive microcoils, high magnetic field, and methods designed to maximize contrast to noise ratio. The presented approach may provide a powerful multimodal tool that merges structural and functional information of submilimeter biological samples.
authors: Department of Radiology, Medical Physics, University Medical Center, Freiburg, Germany. nicoleta.baxan@uniklinik-freiburg.de
authors: Nicoleta
authors: N
authors: Baxan
authors: Ulf
authors: U
authors: Kahlert
authors: Jaroslaw
authors: J
authors: Maciaczyk
authors: Guido
authors: G
authors: Nikkhah
authors: Jürgen
authors: J
authors: Hennig
authors: Dominik
authors: D
authors: von Elverfeldt
mesh_terms: D003287:Contrast Media; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D008345:Manganese; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008904:Miniaturization; D018874:Spheroids, Cellular / Q000473:pathology*; D013223:Statistics as Topic
chemical_list: D003287:Contrast Media; D008345:Manganese