title: Unpairing and gating: sequence-independent substrate recognition by FEN superfamily nucleases.
abstract: Structure-specific 5'-nucleases form a superfamily of evolutionarily conserved phosphodiesterases that catalyse a precise incision of a diverse range of DNA and RNA substrates in a sequence-independent manner. Superfamily members, such as flap endonucleases, exonuclease 1, DNA repair protein XPG, endonuclease GEN1 and the 5'-3'-exoribonucleases, play key roles in many cellular processes such as DNA replication and repair, recombination, transcription, RNA turnover and RNA interference. In this review, we discuss recent results that highlight the conserved architectures and active sites of the structure-specific 5'-nucleases. Despite substrate diversity, a common gating mechanism for sequence-independent substrate recognition and incision emerges, whereby double nucleotide unpairing of substrates is required to access the active site.
authors: Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield S3 7HF, UK. j.a.grasby@sheffield.ac.uk
authors: Jane A
authors: JA
authors: Grasby
authors: L David
authors: LD
authors: Finger
authors: Susan E
authors: SE
authors: Tsutakawa
authors: John M
authors: JM
authors: Atack
authors: John A
authors: JA
authors: Tainer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004260:DNA Repair; D004720:Endonucleases / Q000737:chemistry / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism; D045585:Flap Endonucleases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D034622:RNA Interference; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004720:Endonucleases; D005090:Exodeoxyribonucleases; D045585:Flap Endonucleases; C028450:exodeoxyribonuclease I