title: Studies on guanidinated N-3-aminopropyl methacrylamide-N-2-hydroxypropyl methacrylamide co-polymers as gene delivery carrier.
abstract: Guanidinated N-3-aminopropyl methacrylamide (APMA)-N-2-hydroxypropyl methacrylamide (HPMA) co-polymers were prepared and evaluated to develop novel non-viral gene transfection carriers. The co-polymers were synthesized via radical co-polymerization of APMA and HPMA followed by  total guanidination of amino groups, which employed guanidinated APMA (GPMA) for increasing cell-penetrating and HPMA as the positive shielding content. The molecular weight of guanidinated APMA-HPMA co-polymers (GPMA-HPMA) was determined by static light scattering. Furthermore,  cytotoxicity and transfection experiments of GPMA-HPMA/pDNA complexes were conducted. A significant decrease of their parent cytotoxicity and an efficient transfection at relative low charge ratios were observed. The cellular distribution of most GPMA-HPMA/pDNA complexes was partially  localized in the nucleus, as indicated by confocal laser scanning microscopy. The guanidination strategy employed may lead to non-viral gene delivery carriers that combine satisfactory transfection efficiency and cytotoxicity, which contribute to their cell-penetrating ability.
authors: Biomaterials and Drug Delivery Laboratories, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, P. R. China.
authors: Zhu
authors: Z
authors: Qin
authors: Wei
authors: W
authors: Liu
authors: Liang
authors: L
authors: Guo
authors: Xinsong
authors: X
authors: Li
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D001692:Biological Transport; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D017930:Genes, Reporter / Q000235:genetics; D019791:Guanidine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010316:Particle Size; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D014162:Transfection / Q000379:methods*
chemical_list: D000178:Acrylamides; D004337:Drug Carriers; D011108:Polymers; D004247:DNA; D019791:Guanidine; C045985:methacrylamide