title: Electropolymerized network of polyamidoamine dendron-coated gold nanoparticles as novel nanostructured electrode surface for biosensor construction.
abstract: Polyfuntionalized gold nanoparticles were prepared by using 2-mercaptoethanesulfonic acid, p-aminothiophenol and cysteamine core polyamidoamine G-4 dendron as capping ligands. The nanoparticles were electropolymerized on a Au electrode surface through the formation of a bisaniline-cross-linked network. The enzyme tyrosinase was further crosslinked on this nanostructured matrix. The enzyme electrode, poised at -100 mV, was used for the amperometric quantification of cathecol. The biosensor showed a linear response from 50 nM to 10 μM cathecol, with a low detection limit of 20 nM and a sensitivity of 1.94 A M(-1) cm(2). The electrode retained 96% and 67% of its initial activity after 16 and 30 days of storage at 4 °C under dry conditions.
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors: Reynaldo
authors: R
authors: Villalonga
authors: Paula
authors: P
authors: Díez
authors: Santiago
authors: S
authors: Casado
authors: Marcos
authors: M
authors: Eguílaz
authors: Paloma
authors: P
authors: Yáñez-Sedeño
authors: José M
authors: JM
authors: Pingarrón
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D050091:Dendrimers / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D006046:Gold; D014442:Monophenol Monooxygenase