title: Characterization of serum phospholipase a(2) activity in three diverse species of west african crocodiles.
abstract: Secretory phospholipase A(2), an enzyme that exhibits substantial immunological activity, was measured in the serum of three species of diverse West African crocodiles. Incubation of different volumes of crocodile serum with bacteria labeled with a fluorescent fatty acid in the sn-2 position of membrane lipids resulted in a volume-dependent liberation of fluorescent probe. Serum from the Nile crocodile (Crocodylus niloticus) exhibited slightly higher activity than that of the slender-snouted crocodile (Mecistops cataphractus) and the African dwarf crocodile (Osteolaemus tetraspis). Product formation was inhibited by BPB, a specific PLA(2) inhibitor, confirming that the activity was a direct result of the presence of serum PLA(2). Kinetic analysis showed that C. niloticus serum produced product more rapidly than M. cataphractus or O. tetraspis. Serum from all three species exhibited temperature-dependent PLA(2) activities but with slightly different thermal profiles. All three crocodilian species showed high levels of activity against eight different species of bacteria.
authors: Department of Chemistry, McNeese State University, 450 Beauregard, Kirkman Hall 221A, Lake Charles, LA 70609, USA.
authors: Mark
authors: M
authors: Merchant
authors: Kate
authors: K
authors: Juneau
authors: Jared
authors: J
authors: Gemillion
authors: Rodolfo
authors: R
authors: Falconi
authors: Aaron
authors: A
authors: Doucet
authors: Matthew H
authors: MH
authors: Shirley