title: Effect of enteral nutrition on dextran sulfate sodium-induced colitis in rats.
abstract: OBJECTIVE
  To study the effect of enteral nutrition (EN) on dextran sulfate sodium (DSS)-induced colitis in rats.
  
  
  METHODS
  Eighty-four Sprague-Dawley rats were divided into 7 groups (12 rats in each group). The blank control group was given ordinary laboratory feed and drinking water. The experimental groups received 5% DSS as drinking water for 7 days. Of the experimental groups, the model control group received ordinary laboratory feed, protein based enteral nutrition (PEN) was fed in the PEN group, while other groups received ordinary laboratory feed plus 5-aminosalicylic acid (5-ASA), methyl-prednisolone, Lactobacillus or glutamine, respectively. On the 8th day, all the rats were sacrificed. Inflammatory scores were assessed from colonic mucosa. Blood culture from inferior vena cava, fecal culture and secretary immunoglobulin-A (S-IgA) levels from colonic contents were determined.
  
  
  RESULTS
  Colon inflammatory scores of Lactobacillus, PEN, glutamine and drug-treated groups were lower than that of the model control group (P < 0.01). The ratios of bacteria translocation in the EN (PEN, Lactobacillus and glutamine) groups were lower than that in the model control group (P < 0.0083). Fecal Lactobacilli in the Lactobacillus and glutamine groups were higher than that in the model control group (P < 0.05). S-IgA levels in colonic contents of the PEN and 5-ASA group were lower than that in the model control group (P < 0.05).
  
  
  CONCLUSIONS
  EN is an effective therapy for treating DDS-induced colitis. EN could alleviate damage, promote the repair of colonic epithelial cells and inhibit bacterial translocation. Lactobacillus and glutamine could also increase the Lactobacilli in colon.
authors: Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing Digestive Disease Center, Beijing, China.
authors: Fang
authors: F
authors: Wang
authors: Hai Ying
authors: HY
authors: Zhao
authors: Shu Tian
authors: ST
authors: Zhang
authors: You Zhe
authors: YZ
authors: Gong
authors: Hai Fang
authors: HF
authors: Zhang
authors: Chuan
authors: C
authors: Zhang
mesh_terms: D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced* / Q000178:diet therapy* / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000382:microbiology; D016264:Dextran Sulfate / Q000009:adverse effects*; D004750:Enteral Nutrition; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005973:Glutamine / Q000494:pharmacology; D007070:Immunoglobulin A / Q000378:metabolism; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification; D008297:Male; D023421:Models, Animal; D019936:Probiotics / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007070:Immunoglobulin A; D005973:Glutamine; D016264:Dextran Sulfate