title: The aqueous garlic, onion and leek extracts release nitric oxide from S-nitrosoglutathione and prolong relaxation of aortic rings.
abstract: Garlic, onion and leek have beneficial effects in treatment of numerous health disorders. The aim of the present study was to investigate underlying molecular mechanisms. To test the potency of the aqueous garlic, onion and leek extracts to release NO from GSNO we have measured NO oxidation product, NO(2)-, by the Griess reagent method. Further, we studied the ability of garlic extract to relax noradrenaline-precontracted rat aortic rings in the presence of GSNO and effects of garlic extract on electrical properties of rat heart intracellular chloride channels. We have observed that: i) garlic, onion and leek extracts released NO from GSNO in the order: garlic > onion > leek; ii) the ability of garlic extract to release NO was pH-dependent (8.0 > 7.4 > 6.0) and potentiated by thiols (Cys >> GSH = N-acetyl-cysteine > oxidized glutathione) at concentration 100 µmol/l; iii) the garlic extract (0.045 mg/ml) prolonged relaxation time of aortic rings induced by GSNO (50 nmol/l) and inhibited intracellular chloride channels. We suggest that NO-releasing properties of the garlic, onion and leek extracts and their interaction with Cys and GSH are involved in NO-signalling pathway which contributes to some of its numerous beneficial biological effects.
authors: Physics and Informatics, Comenius University, Bratislava, Slovak Republic.
authors: Marian
authors: M
authors: Grman
authors: Anton
authors: A
authors: Misak
authors: Sona
authors: S
authors: Cacanyiova
authors: Frantisek
authors: F
authors: Kristek
authors: Zuzana
authors: Z
authors: Tomaskova
authors: Anna
authors: A
authors: Bertova
authors: Karol
authors: K
authors: Ondrias
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology*; D018118:Chloride Channels / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004730:Endothelium, Vascular / Q000473:pathology; D005737:Garlic / Q000378:metabolism*; D005978:Glutathione / Q000737:chemistry; D019803:Glutathione Disulfide / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008297:Male; D009569:Nitric Oxide / Q000737:chemistry*; D009638:Norepinephrine / Q000494:pharmacology; D019697:Onions / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D026422:S-Nitrosoglutathione / Q000378:metabolism*
chemical_list: D018118:Chloride Channels; D008051:Lipid Bilayers; D010936:Plant Extracts; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D005978:Glutathione; D003545:Cysteine; D019803:Glutathione Disulfide; D009638:Norepinephrine