title: Characterization of avian paramyxovirus type 1 from migratory wild birds in chickens.
abstract: Newcastle disease virus (NDV) is one of the most important infectious agents in the poultry industry, and vaccines against it have been widely used for prevention and control. Live vaccines, which can replicate in the respiratory and digestive systems, have been especially needed in areas with outbreaks of viscerotropic velogenic Newcastle disease. Towards the goal of searching for a new live vaccine candidate, avian paramyxovirus type 1 (APMV-1) was isolated from the faeces of wild birds. Three APMV-1 strains thus isolated were characterized in terms of phylogeny, pathogenicity, immunogenicity and tissue tropism, and on the basis of these analyses were classified as lentogenic genotype I NDV. CBU2179, one of the three APMV-1 strains, was selected and was evaluated in terms of its efficacy and safety in specific pathogen-free chickens and commercial broilers. The manufactured trial vaccine from this strain, also called CBU2179, induced similar immune responses to those of VG/GA and B1 commercial vaccines, and provided 100% protection against challenge from viscerotropic velogenic NDV, KJW/49 strain (the official challenge strain in Korea). Also, the CBU2179 virus was re-isolated and persisted as long as or longer than other vaccine strains in both the respiratory and alimentary tracts. Therefore, the CBU2179 strain may represent a good candidate for a live Newcastle disease vaccine to protect chickens against viscerotropic velogenic NDV.
authors: College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
authors: Jong-Bo
authors: JB
authors: Shim
authors: Hyun-Hee
authors: HH
authors: So
authors: Ho-Keun
authors: HK
authors: Won
authors: In-Pil
authors: IP
authors: Mo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D016000:Cluster Analysis; D004372:Ducks / Q000821:virology*; D005243:Feces / Q000821:virology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013047:Specific Pathogen-Free Organisms; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology*
chemical_list: D014765:Viral Vaccines