title: Simultaneous saccharification and fermentation of acid-pretreated rapeseed meal for succinic acid production using Actinobacillus succinogenes.
abstract: Rapeseed meal was evaluated for succinic acid production by simultaneous saccharification and fermentation using Actinobacillus succinogenes ATCC 55618. Diluted sulfuric acid pretreatment and subsequent hydrolysis with pectinase was used to release sugars from rapeseed meal. The effects of culture pH, pectinase loading and yeast extract concentration on succinic acid production were investigated. When simultaneous saccharification and fermentation of diluted acid pretreated rapeseed meal with a dry matter content of 12.5% (w/v) was performed at pH 6.4 and a pectinase loading of 2% (w/w, on dry matter) without supplementation of yeast extract, a succinic acid concentration of 15.5 g/L was obtained at a yield of 12.4 g/100g dry matter. Fed-batch simultaneous saccharification and fermentation was carried out with supplementation of concentrated pretreated rapeseed meal and pectinase at 18 and 28 h to yield a final dry matter content of 20.5% and pectinase loading of 2%, with the succinic acid concentration enhanced to 23.4 g/L at a yield of 11.5 g/100g dry matter and a productivity of 0.33 g/(Lh). This study suggests that rapeseed meal may be an alternative substrate for the efficient production of succinic acid by A. succinogenes without requiring nitrogen source supplementation.
authors: Faculty of Bioresource Sciences, Akita Prefectural University, Kaidobata-Nishi 241-438, Shimoshinjo-Nakano, Akita-shi, Akita 010-0195, Japan.
authors: Kequan
authors: K
authors: Chen
authors: Han
authors: H
authors: Zhang
authors: Yelian
authors: Y
authors: Miao
authors: Ping
authors: P
authors: Wei
authors: Jieyu
authors: J
authors: Chen
mesh_terms: D000188:Actinobacillus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D029741:Brassica rapa / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D011096:Polygalacturonase / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D013464:Sulfuric Acids / Q000494:pharmacology
chemical_list: D003470:Culture Media; D013464:Sulfuric Acids; D019802:Succinic Acid; D011096:Polygalacturonase; C033158:sulfuric acid