title: DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to methylmercury.
abstract: To investigate the adaptive response to the environmental electrophile methylmercury (MeHg), we performed DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to a sub-cytotoxic dose of MeHg (1 µM) for 6 hr. The expression of 15 genes increased 10-fold or more in response to MeHg. Four of these genes are associated with detoxification and excretion of MeHg into the extracellular space, and are regulated by transcription factor Nrf2 through the electrophile response element. Interestingly, Cullin3, a negative regulator of Nrf2, was identified as a down-regulated gene during MeHg exposure.
authors: Doctoral Program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors: Takashi
authors: T
authors: Toyama
authors: Eiko
authors: E
authors: Yoshida
authors: Yasuhiro
authors: Y
authors: Shinkai
authors: Yoshito
authors: Y
authors: Kumagai
mesh_terms: D027182:Amino Acid Transport System y+ / Q000235:genetics; D045744:Cell Line, Tumor; D044842:Cullin Proteins / Q000235:genetics; D015536:Down-Regulation; D004785:Environmental Pollutants / Q000633:toxicity*; D020869:Gene Expression Profiling; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D008767:Methylmercury Compounds / Q000633:toxicity*; D046228:Microarray Analysis; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009447:Neuroblastoma; D015854:Up-Regulation
chemical_list: D027182:Amino Acid Transport System y+; D044842:Cullin Proteins; D004785:Environmental Pollutants; D051547:Heme Oxygenase-1; D008767:Methylmercury Compounds; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016660:NAD(P)H Dehydrogenase (Quinone); D051267:NF-E2-Related Factor 2; C503645:CUL3 protein, human; C495635:NFE2L2 protein, human; C118895:SLC7A11 protein, human; C114462:multidrug resistance-associated protein 3; C496541:HMOX1 protein, human; C447479:NQO1 protein, human