title: Intracellular Ca Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells.
abstract: The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca(2+) mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca(2+) concentration. The increase of intracellular Ca(2+) induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3±2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 µM melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca(2+) mobilization and cellular function in RBL 2H3 cells.
authors: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors: Yeon Hee
authors: YH
authors: Hwang
authors: Ho Sun
authors: HS
authors: Song
authors: Hee Rae
authors: HR
authors: Kim
authors: Myoung Soo
authors: MS
authors: Ko
authors: Jae Min
authors: JM
authors: Jeong
authors: Yong Ho
authors: YH
authors: Kim
authors: Jeong Soo
authors: JS
authors: Ryu
authors: Uy Dong
authors: UD
authors: Sohn
authors: Yoon-Myoung
authors: YM
authors: Gimm
authors: Sung Ho
authors: SH
authors: Myung
authors: Sang Soo
authors: SS
authors: Sim