title: Role of -675 4G/5G in the plasminogen activator inhibitor-1 gene and -308G/A tumor necrosis factor-α gene polymorphisms in obese Argentinean patients.
abstract: OBJECTIVE
  Plasminogen activator inhibitor-1 (PAI-1) and tumor necrosis factor-α (TNF-α) are increased in the circulation of obese persons. Because a direct link between PAI-1 and TNF-α in obesity has been observed, they are candidate genes for the development of obesity. We sought to evaluate the relation between the genotypic and allelic frequencies of the -675 4G/5G PAI-1 and -308 G/A TNF-α polymorphisms and their association with the risk for obesity in an Argentinean population.
  
  
  METHODS
  A group of 110 consecutive obese persons and a group of 111 lean controls were recruited. Polymerase chain reaction was used to determine the frequency of PAI-1 and TNF-α polymorphisms; serum fasting glucose, insulin, and lipid levels were measured by standard methods. Insulin sensitivity was evaluated by using homeostasis model assessment.
  
  
  RESULTS
  The -308 TNF-α and -675 4G/5G PAI-1 genotype distribution did not significantly differ between the groups (p=0.544 and p=0.327, respectively). Homeostasis model assessment was the only positive independent determinant of body mass index (R(2)=0.493; p<0.001).
  
  
  CONCLUSIONS
  The -675 4G/5G PAI-1 and the -308 TNF-α polymorphism variants tested in this study, individually or combined, were not associated with obesity in an Argentinean population.
authors: Hemostasis and Thrombosis Laboratory, Hospital of Infectious Diseases F.J. Muñiz, Buenos Aires, Argentina. sdaperes@yahoo.com.ar
authors: Silvia D Perés
authors: SD
authors: Wingeyer
authors: Mabel N
authors: MN
authors: Graffigna
authors: Susana H
authors: SH
authors: Belli
authors: Jorge
authors: J
authors: Benetucci
authors: Gabriela F
authors: GF
authors: de Larrañaga
mesh_terms: D000328:Adult; D001118:Argentina; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D009765:Obesity / Q000235:genetics*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D017395:Plasminogen Activator Inhibitor 1; D014409:Tumor Necrosis Factor-alpha