title: Nucleolin interacts with US11 protein of herpes simplex virus 1 and is involved in its trafficking.
abstract: Herpes simplex virus type 1 (HSV-1) infection induces profound nucleolar modifications at the functional and organizational levels, including nucleolar invasion by several viral proteins. One of these proteins is US11, which exhibits several different functions and displays both cytoplasmic localization and clear nucleolar localization very similar to that of the major multifunctional nucleolar protein nucleolin. To determine whether US11 interacts with nucleolin, we purified US11 protein partners by coimmunoprecipitations using a tagged protein, Flag-US11. From extracts of cells expressing Flag-US11 protein, we copurified a protein of about 100 kDa that was further identified as nucleolin. In vitro studies have demonstrated that nucleolin interacts with US11 and that the C-terminal domain of US11, which is required for US11 nucleolar accumulation, is sufficient for interaction with nucleolin. This association was confirmed in HSV-1-infected cells. We found an increase in the nucleolar accumulation of US11 in nucleolin-depleted cells, thereby revealing that nucleolin could play a role in US11 nucleocytoplasmic trafficking through one-way directional transport out of the nucleolus. Since nucleolin is required for HSV-1 nuclear egress, the interaction of US11 with nucleolin may participate in the outcome of infection.
authors: Université de Lyon and Université Lyon 1, Lyon, France.
authors: Anna
authors: A
authors: Greco
authors: Loredana
authors: L
authors: Arata
authors: Eric
authors: E
authors: Soler
authors: Xavier
authors: X
authors: Gaume
authors: Yohann
authors: Y
authors: Couté
authors: Sabine
authors: S
authors: Hacot
authors: Aleth
authors: A
authors: Callé
authors: Karine
authors: K
authors: Monier
authors: Alberto L
authors: AL
authors: Epstein
authors: Jean-Charles
authors: JC
authors: Sanchez
authors: Philippe
authors: P
authors: Bouvet
authors: Jean-Jacques
authors: JJ
authors: Diaz
mesh_terms: D026481:Blotting, Far-Western; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D000097796:Nucleolin
chemical_list: D010750:Phosphoproteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D000097796:Nucleolin; C074546:US11 protein, herpesvirus