title: [Inhibition of glutathione-S-transferase by total saponins of Panax notoginseng and its kinetics analysis in liver of mice].
abstract: OBJECTIVE
  To study the inhibition of glutathione-s-transferase by total saponins of Panax notoginseng and its kinetics analysis in liver of mice.
  
  
  METHODS
  Mouse liver cytochyma enzyme was obtained by different velocity centrifugation, the mouse liver glutathione-S-transferase of michaelis constant (Km), maximum velocity (Vmax) and the inhibition of glutathione-S-transferase by total saponins of P. notoginseng of 50% inhibiting concentration (IC50), inhibition constant (KI, KIS), the type of inhibition were calculation by Lineweaver-Burk and the low of semi-effet-probit.
  
  
  RESULTS
  It was found that total saponins of P. notoginseng inhibited the glutathione-S-transferase activity with IC50 of 189.54 mg x L(-1). Kinetics analysis showed the glutathione-S-transferase of Km was 0.4563 mmol x L(-1) and Vmax was 476.19 U x mg(-1) with reduced glutathione (GSH) substrate, 0.1097 mmol x L(-1) (Km) and 400.00 U x mg(-1) (Vmax) with 1-chloro-2,4-dinitrobenzene (CDNB) substrate. Kinetics studies of total saponins of P. notoginseng on glutathione-S-transferase showed the inhibition were belong to mix-type with GSH and CDNB; the inhibition constant was 0.27 mg x L(-1) (KI), 0.68 mg x L(-1) (KIS) with GSH, and 0.21 mg x L(-1) (KI), 0.66 mg x L(-1) (KIS) with CDNB.
  
  
  CONCLUSIONS
  Total saponins of P. notoginseng strongly inhibited the glutathione-S-transferase activity.
authors: Department of Pharmacology, Faculty of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning 530001, China.
authors: Ziming
authors: Z
authors: Yang
authors: Xiufen
authors: X
authors: Yang
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D004791:Enzyme Inhibitors / Q000493:pharmacokinetics*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D053723:Panax notoginseng / Q000737:chemistry*; D012503:Saponins / Q000493:pharmacokinetics*
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D012503:Saponins; D005982:Glutathione Transferase