title: Activation of microbubbles by short-pulsed ultrasound enhances the cytotoxic effect of cis-diamminedichloroplatinum (II) in a canine thyroid adenocarcinoma cell line in vitro.
abstract: Ultrasound targeted microbubble destruction has succeeded in delivering drugs and genes. This study was designed to explore characteristics of ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. Canine thyroid adenocarcinoma cells were exposed to short-pulsed diagnostic ultrasound in the presence of cis-diamminedichloroplatinum (II) (cisplatin) and ultrasound contrast agent Sonazoid(®) microbubbles. The cytotoxic effect of cisplatin was enhanced by short-pulsed diagnostic ultrasound and microbubbles. Incubation time with microbubbles influenced the cytotoxic effect of cisplatin. However, exposure duration did not affect the cytotoxic effect of cisplatin. Therefore, short-pulsed diagnostic ultrasound may activate microbubbles near cells and deliver cisplatin into cells. In addition, activation of microbubbles may be concluded in a short time. Our results suggest that short exposure duration could be potentially sufficient to induce efficient drug delivery by ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound.
authors: Laboratory of Veterinary Internal Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.
authors: Noboru
authors: N
authors: Sasaki
authors: Nobuki
authors: N
authors: Kudo
authors: Kensuke
authors: K
authors: Nakamura
authors: Sue Yee
authors: SY
authors: Lim
authors: Masahiro
authors: M
authors: Murakami
authors: W R Bandula
authors: WR
authors: Kumara
authors: Yu
authors: Y
authors: Tamura
authors: Hiroshi
authors: H
authors: Ohta
authors: Masahiro
authors: M
authors: Yamasaki
authors: Mitsuyoshi
authors: M
authors: Takiguchi
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology* / Q000628:therapy; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D002945:Cisplatin / Q000008:administration & dosage*; D003287:Contrast Media / Q000528:radiation effects / Q000627:therapeutic use; D004285:Dogs; D004357:Drug Synergism; D005290:Ferric Compounds / Q000528:radiation effects* / Q000627:therapeutic use*; D019220:High-Energy Shock Waves; D007501:Iron / Q000528:radiation effects* / Q000627:therapeutic use*; D045423:Microbubbles; D010087:Oxides / Q000528:radiation effects* / Q000627:therapeutic use*; D013964:Thyroid Neoplasms / Q000503:physiopathology* / Q000628:therapy; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D005290:Ferric Compounds; D010087:Oxides; C069727:Sonazoid; D007501:Iron; D002945:Cisplatin