title: Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group.
abstract: Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.
authors: Reproductive Services, Royal Women's Hospital, Grattan Street & Flemington Road, Parkville, Victoria, 3052, Australia. debra.gook@mivf.com.au
authors: Debra A
authors: DA
authors: Gook
authors: David H
authors: DH
authors: Edgar
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D010064:Embryo Implantation / Q000502:physiology*; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D005260:Female; D006801:Humans; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D019946:Propylene Glycol / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D013395:Sucrose; D019946:Propylene Glycol