title: A study in vitro on differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in mice.
abstract: OBJECTIVE
  To investigate the differentiation conditions of bone marrow mesenchymal stem cells (BMSCs) into endometrial epithelial cells and to confirm the effect of 17β-estradiol in this process.
  
  
  METHODS
  BMSCs were cultured alone or co-cultured with endometrial stromal cells (EStCs) in control/differentiation medium (17β-estradiol, growth factors) and were co-cultured with EStCs in different concentrations of 17β-estradiol. Flow cytometry and immunocytochemistry were used to identify the isolated cells. Real-time RT-PCR and immunofluorescence were used to test the expression of epithelial cell markers.
  
  
  RESULTS
  The epithelial markers cytokeratin-7, cytokeratin-18, cytokeratin-19, and epithelial membrane antigen were elevated in real-time RT-PCR (P<0.05), and cytokeratin was strongly positive in immunofluorescence analysis in the differentiated BMSCs. Cytokeratin-7 and cytokeratin-19 expression levels were highest in the 1 × 10⁻⁸ mol/L 17β-estradiol group, as shown in real-time RT-PCR (P<0.05).
  
  
  CONCLUSIONS
  BMSCs could be differentiated in the direction of endometrial epithelial cells in appropriate conditions in vitro: 17β-estradiol may play a key role in stimulating BMSCs' epithelial differentiation in the process of endometriosis.
  
  
  CONCLUSIONS
  Bone marrow mesenchymal stem cells can differentiate in the direction of endometrial epithelial cells in a certain microenvironment and appropriate concentration of 17β-E₂ can facilitate this differentiation.
authors: Obstetrics and Gynecology Hospital, Department of Obstetrics and Gynecology of Shanghai Medical School, and Institute of Biomedical Sciences, Fudan University, Shanghai, PR China.
authors: Wen-Bi
authors: WB
authors: Zhang
authors: Ming-Jun
authors: MJ
authors: Cheng
authors: Yu-Ting
authors: YT
authors: Huang
authors: Wei
authors: W
authors: Jiang
authors: Qing
authors: Q
authors: Cong
authors: Yu-Fang
authors: YF
authors: Zheng
authors: Cong-Jian
authors: CJ
authors: Xu
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism; D009994:Osmolar Concentration; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D055153:Stem Cell Niche
chemical_list: D000943:Antigens, Differentiation; D004967:Estrogens; D018396:Mucin-1; D020033:Protein Isoforms; D004958:Estradiol; D007633:Keratins