title: Probiotic genomic DNA reduces the production of pro-inflammatory cytokine tumor necrosis factor-alpha.
abstract: The effect of Lactobacillus plantarum genomic DNA on lipopolysaccharide (LPS)-induced mitogen-activated protein kinase (MAPK) activation, nuclear factor-kappa B activation, and the expressions of tumor necrosis factor-alpha, interleukin-1 receptor-associated kinase M, and the pattern recognition receptor were examined. Pretreatment of p-gDNA inhibited the phosphorylation of MAPKs and nuclear factor-kappa B, and also inhibited LPS-induced TNF-α production in response to subsequent LPS stimulation. L. plantarum genomic DNA-mediated inhibition of signaling pathway and tumor necrosis factor-alpha was accompanied by the suppression of toll-like receptor (TLR) 2, TLR4, and TLR9 and the induction of interleukin-1 receptor-associated kinase M, a negative regulator of TLR. This study can extend our understanding of the biological function of probiotic genomic DNA as an anti-inflammatory agent.
authors: School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, South Korea.
authors: Chung Hee
authors: CH
authors: Kim
authors: Han Geun
authors: HG
authors: Kim
authors: Joo Yun
authors: JY
authors: Kim
authors: Na Ra
authors: NR
authors: Kim
authors: Bong Jun
authors: BJ
authors: Jung
authors: Ji Hye
authors: JH
authors: Jeong
authors: Dae Kyun
authors: DK
authors: Chung
mesh_terms: D002460:Cell Line; D004247:DNA / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology*; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis; D051217:Toll-Like Receptor 9 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000276:immunology*
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; C495342:TLR2 protein, human; C495345:TLR4 protein, human; C495371:TLR9 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; D004247:DNA; D053592:Interleukin-1 Receptor-Associated Kinases; D020929:Mitogen-Activated Protein Kinase Kinases