title: The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis.
abstract: Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.
authors: Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Osong, Republic of Korea. blackcg96@gmail.com
authors: Myoung-Ro
authors: MR
authors: Lee
authors: Yu-Jung
authors: YJ
authors: Kim
authors: Dae-Won
authors: DW
authors: Kim
authors: Won Gi
authors: WG
authors: Yoo
authors: Shin-Hyeong
authors: SH
authors: Cho
authors: Kwang Yeon
authors: KY
authors: Hwang
authors: Jung-Won
authors: JW
authors: Ju
authors: Won-Ja
authors: WJ
authors: Lee
mesh_terms: D048948:14-3-3 Proteins / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D003001:Cloning, Molecular; D003003:Clonorchiasis / Q000276:immunology / Q000469:parasitology; D003004:Clonorchis sinensis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018260:Gelsolin / Q000276:immunology / Q000302:isolation & purification; D005982:Glutathione Transferase / Q000276:immunology / Q000302:isolation & purification; D015801:Helminth Proteins / Q000276:immunology; D006801:Humans; D043586:Methylmalonyl-CoA Decarboxylase / Q000276:immunology / Q000302:isolation & purification*; D000091402:Prohibitins; D011994:Recombinant Proteins / Q000276:immunology; D012097:Repressor Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D048948:14-3-3 Proteins; D000947:Antigens, Helminth; D015415:Biomarkers; D018260:Gelsolin; D015801:Helminth Proteins; D000091402:Prohibitins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D005982:Glutathione Transferase; D043586:Methylmalonyl-CoA Decarboxylase