title: Antimicrobial and antibiofilm action of Casbane Diterpene from Croton nepetaefolius against oral bacteria.
abstract: OBJECTIVE
  The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis, S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm action was assayed.
  
  
  METHODS
  The minimal inhibitory and bactericidal concentrations (MIC and MBC) of oral Streptococcus were evaluated through microdilution tests. To assay antibiofilm activity, biofilms were generated on 96-wells polystyrene plates under the presence of CD and quantified by a crystal violet technique and colonies forming units counting.
  
  
  RESULTS
  The CD isolated from Croton nepetaefolius showed antimicrobial effect on planktonic forms and biofilms of oral pathogens, with MIC values of 62.5 μg/mL against Streptococcus oralis and values between 125 and 500 μg/mL against S. mutans, S. salivarius, S. sobrinus, S. mitis and S. sanguinis. CD showed an inhibitory effect on S. mutans biofilm formation at 250 μg/mL, and a decrease on viable cell of 94.28% compared to the normal biofilm growth.
  
  
  CONCLUSIONS
  The compound CD can be considered as a promising molecule for the treatment against oral pathogens responsible for dental biofilm.
authors: LIBS, Integrate Biomolecules Laboratory, Federal University of Ceará, Sobral, CE, Brazil.
authors: Nairley
authors: N
authors: Cardoso Sá
authors: Theodora Thays Arruda
authors: TT
authors: Cavalcante
authors: Amanda Ximenes
authors: AX
authors: Araújo
authors: Hélcio Silva
authors: HS
authors: dos Santos
authors: Maria Rose Jane Ribeiro
authors: MR
authors: Albuquerque
authors: Paulo Nogueira
authors: PN
authors: Bandeira
authors: Rodrigo Maranguape Silva
authors: RM
authors: da Cunha
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: Edson Holanda
authors: EH
authors: Teixeira
mesh_terms: D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D015169:Colony Count, Microbial; D029802:Croton; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D005840:Gentian Violet; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013291:Streptococcus / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D004224:Diterpenes; D010936:Plant Extracts; D005840:Gentian Violet