title: Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle.
abstract: The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.
authors: The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, 41470 Gebze, Kocaeli, Turkey. sezen.arat@mam.gov.tr
authors: Sezen
authors: S
authors: Arat
authors: Arzu T
authors: AT
authors: Caputcu
authors: Tolga
authors: T
authors: Akkoc
authors: Serhat
authors: S
authors: Pabuccuoglu
authors: Hakan
authors: H
authors: Sagirkaya
authors: Umut
authors: U
authors: Cirit
authors: Yavuz
authors: Y
authors: Nak
authors: Evren
authors: E
authors: Koban
authors: Haydar
authors: H
authors: Bagis
authors: Kamber
authors: K
authors: Demir
authors: Deniz
authors: D
authors: Nak
authors: Adem
authors: A
authors: Senunver
authors: Ragip
authors: R
authors: Kilicaslan
authors: Bilginer
authors: B
authors: Tuna
authors: Gaye
authors: G
authors: Cetinkaya
authors: Melis
authors: M
authors: Denizci
authors: Ozgur
authors: O
authors: Aslan
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods*; D002356:Cartilage / Q000166:cytology*; D002417:Cattle; D002460:Cell Line; D019976:Cloning, Organism / Q000379:methods*; D015925:Cryopreservation; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006107:Granulosa Cells / Q000166:cytology*; D006239:Haplotypes / Q000235:genetics; D008297:Male; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D016615:Telomere / Q000235:genetics; D014015:Tissue Banks
chemical_list: D004272:DNA, Mitochondrial