title: Harmonising measurements of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA and urine.
abstract: Levels of oxidatively damaged cellular DNA and urinary excretion of damaged 2'-deoxyribonuclosides are widely measured in biomonitoring studies examining the role of oxidative stress induced by environmental exposures, lifestyle factors and development of disease. This has promoted efforts to harmonise measurements of oxidised guanine nucleobases by the variety of analytical approaches for DNA and urinary levels of damage, in multi-laboratory trials that are centred in Europe. The large inter-laboratory variation reported of values of oxidatively damaged DNA is reduced by harmonising assay protocols. Recent attention on optimal conditions for the comet assay may lead to better understanding of the most critical steps in procedure, which generate variation in DNA damage levels between laboratories. Measurements of urinary excretion of oxidatively generated 8-oxo-7,8-dihydro-2'-deoxyguanosine also show large differences between different methods, where chromatographic techniques generally show more reliable results than antibody-based methods. In this case, standardising calibrants is aimed at improving within technique agreement.
authors: Section of Environmental Health, Department of Public Health, University of Copenhagen, Denmark. pemo@sund.ku.dk
authors: Peter
authors: P
authors: Møller
authors: Marcus S
authors: MS
authors: Cooke
authors: Andrew
authors: A
authors: Collins
authors: Ryszard
authors: R
authors: Olinski
authors: Rafal
authors: R
authors: Rozalski
authors: Steffen
authors: S
authors: Loft
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine*; D004247:DNA / Q000737:chemistry* / Q000652:urine*; D004249:DNA Damage; D003839:Deoxyadenosines / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010084:Oxidation-Reduction
chemical_list: D015415:Biomarkers; D003839:Deoxyadenosines; C078251:2'-deoxy-7,8-dihydro-8-oxoadenosine; D004247:DNA