title: The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues.
abstract: The lignin-modifying enzymes (LMEs) play an important role in decomposition of agricultural residues, which contain a certain amount of lignin. In this study, the production of LMEs by three co-cultivated combinations of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora and the respective monocultures was comparatively investigated. Laccase and manganese peroxidases (MnP) were significantly promoted in the co-culture of P. radiata and D. squalens, and corncob was verified to be beneficial for laccase and MnP production. Moreover, laccase production by co-culture of P. radiata and D. squalens with high ratio of glucose to nitrogen was higher than low ratio under carbon- and nitrogen-meager conditions. New laccase isoenzymes measured by Native-PAGE were stimulated by co-cultured P. radiata with D. squalens or C. subvermispora, respectively, growing in the defined medium containing corncob, but the expression of laccase was greatly restrained by the co-culturing of D. squalens with C. subvermispora. This study showed that the synergistic and depressing effects of co-cultivation of P. radiata, D. squalens and C. subvermispora on LMEs were species specific.
authors: Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China.
authors: Ya-Chen
authors: YC
authors: Dong
authors: Wei
authors: W
authors: Wang
authors: Zhong-Ce
authors: ZC
authors: Hu
authors: Ming-Liang
authors: ML
authors: Fu
authors: Qi-He
authors: QH
authors: Chen
mesh_terms: D018920:Coculture Techniques; D055453:Coriolaceae / Q000201:enzymology* / Q000254:growth & development*; D005656:Fungal Proteins / Q000096:biosynthesis*; D042845:Laccase / Q000096:biosynthesis*; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000096:biosynthesis*
chemical_list: D005656:Fungal Proteins; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase