title: Investigation of VUV Photodissociation Propensities Using Peptide Libraries.
abstract: PSD does not usually generate a complete series of y-type ions, particularly at high mass, and this is a limitation for de novo sequencing algorithms. It is demonstrated that b(2) and b(3) ions can be used to help assign high mass x(N-2) and x(N-3) fragments that are found in vacuum ultraviolet (VUV) photofragmentation experiments. In addition, v(N)-type ion fragments with side chain loss from the N-terminal residue often enable confirmation of N-terminal amino acids. Libraries containing several thousand peptides were examined using photodissociation in a MALDI-TOF/TOF instrument. 1345 photodissociation spectra with a high S/N ratio were interpreted.
authors: Department of Chemistry, Indiana University, Bloomington, Indiana 47405, and School of Informatics and Computing, Indiana University, Bloomington, Indiana 47408.
authors: Xiaohui
authors: X
authors: Liu
authors: Yong Fuji
authors: YF
authors: Li
authors: Brian C
authors: BC
authors: Bohrer
authors: Randy J
authors: RJ
authors: Arnold
authors: Predrag
authors: P
authors: Radivojac
authors: Haixu
authors: H
authors: Tang
authors: James P
authors: JP
authors: Reilly