title: MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization.
abstract: The inner membrane of mitochondria is especially protein rich and displays a unique morphology characterized by large invaginations, the mitochondrial cristae, and the inner boundary membrane, which is in proximity to the outer membrane. Mitochondrial inner membrane proteins appear to be not evenly distributed in the inner membrane, but instead organize into functionally distinct subcompartments. It is unknown how the organization of the inner membrane is achieved. We identified MINOS1/MIO10 (C1orf151/YCL057C-A), a conserved mitochondrial inner membrane protein. mio10-mutant yeast cells are affected in growth on nonfermentable carbon sources and exhibit altered mitochondrial morphology. At the ultrastructural level, mutant mitochondria display loss of inner membrane organization. Proteomic analyses reveal MINOS1/Mio10 as a novel constituent of Mitofilin/Fcj1 complexes in human and yeast mitochondria. Thus our analyses reveal new insight into the composition of the mitochondrial inner membrane organizing machinery.
authors: Department of Biochemistry II, University of Göttingen Medical School, D-37073 Göttingen, Germany.
authors: Alwaleed K
authors: AK
authors: Alkhaja
authors: Daniel C
authors: DC
authors: Jans
authors: Miroslav
authors: M
authors: Nikolov
authors: Milena
authors: M
authors: Vukotic
authors: Oleksandr
authors: O
authors: Lytovchenko
authors: Fabian
authors: F
authors: Ludewig
authors: Wolfgang
authors: W
authors: Schliebs
authors: Dietmar
authors: D
authors: Riedel
authors: Henning
authors: H
authors: Urlaub
authors: Stefan
authors: S
authors: Jakobs
authors: Markus
authors: M
authors: Deckers
mesh_terms: D000595:Amino Acid Sequence; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure*; D051336:Mitochondrial Membranes / Q000201:enzymology / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C542160:Fcj1 protein, S cerevisiae; C088548:IMMT protein, human; C573102:MIC10 protein, S cerevisiae; C573118:MINOS1 protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009124:Muscle Proteins; D029701:Saccharomyces cerevisiae Proteins; D006180:Proton-Translocating ATPases