title: Cytogenetic instability of dental pulp stem cell lines.
abstract: Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. The heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.
authors: Department of Plastic Surgery, Federal University of São Paulo, UNIFESP CTCMol, Center of Cellular and Molecular Therapy, São Paulo, Brazil.
authors: Monica Talarico
authors: MT
authors: Duailibi
authors: Leslie Domenici
authors: LD
authors: Kulikowski
authors: Silvio Eduardo
authors: SE
authors: Duailibi
authors: Monica Vannucci Nunes
authors: MV
authors: Lipay
authors: Maria Isabel
authors: MI
authors: Melaragno
authors: Lydia Masako
authors: LM
authors: Ferreira
authors: Joseph Phillip
authors: JP
authors: Vacanti
authors: Pamela Crotty
authors: PC
authors: Yelick
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D003782:Dental Pulp / Q000648:ultrastructure*; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping