title: Detection of superoxide anion radical in a stratum corneum intercellular lipid model using an electrochemical sensor.
abstract: A stratum corneum intercellular lipid model was prepared in a quasi-non-aqueous system. It was found that the detection of the superoxide anion radical (O₂⁻•) generated in the lipid model by ultraviolet (UV) irradiation was possible using an electrochemical O₂⁻• sensor. The use of an electron spin resonance-spin trap method confirmed that the reactive oxygen species generated in the lipid model by UV irradiation was O₂⁻•; the presence of a hydroxyl radical (•OH) was also proven. In addition, a reduction in the electric current in the O₂⁻• sensor was observed in lipid models containing added antioxidants such as d-α-tocopherol and β-carotene. Moreover, there was a correlation between the degree of oxidative degradation of the lipid, which was determined by the thiobarbituric acid method, and the electric current due to the O₂⁻• detected using the O₂⁻• sensor.
authors: L. V. M. C. Inc., Tokyo, Japan.
authors: Teruhisa
authors: T
authors: Kaneko
authors: Chihiro
authors: C
authors: Kaise
authors: Yoko
authors: Y
authors: Kimoto
authors: Shota
authors: S
authors: Suzuki
authors: Takeshi
authors: T
authors: Kondo
authors: Makoto
authors: M
authors: Yuasa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004578:Electron Spin Resonance Spectroscopy; D004817:Epidermis / Q000378:metabolism* / Q000528:radiation effects; D005609:Free Radicals; D015227:Lipid Peroxidation / Q000528:radiation effects; D008055:Lipids / Q000737:chemistry*; D008954:Models, Biological; D019097:Spin Trapping / Q000379:methods*; D013481:Superoxides / Q000032:analysis*; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D008055:Lipids; D013481:Superoxides