title: Infection dynamics of Torque teno sus virus types 1 and 2 in serum and peripheral blood mononuclear cells.
abstract: This study was carried out to investigate the presence of Torque teno sus virus types 1 (TTSuV1) and 2 (TTSuV2) in a longitudinally (14 to 150 days of age) collected paired pooled sera (pSE) and peripheral blood mononuclear cells (pPBMCs) using nested polymerase chain reaction. The detection rate of TTSuV1 in pSE increased from 14 to 90 days of age, but a progressive decline was observed from 120 to 150 days of age, while in pPBMC, a high value was maintained till the end of growing-finishing period. On the contrary, except in PBMCs at 30 days of age, high detection rates of TTSuV2 were found in both pSE and pPBMCs in all sampling ages. The detection rate of TTSuVs between pSE and pPBMCs was positively correlated at all sampling ages except for TTSuV1 at 150 days of age. This is the first study showing the presence of TTSuVs in PBMCs from pigs and describing the in vivo infection dynamics of TTSuV in paired sera and PBMCs during the entire growing and finishing periods of pigs reared in conventional farms.
authors: United Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
authors: Chenga
authors: C
authors: Tshering
authors: Mitsuhiro
authors: M
authors: Takagi
authors: Eisaburo
authors: E
authors: Deguchi
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D004266:DNA Virus Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D007963:Leukocytes, Mononuclear / Q000821:virology; D008137:Longitudinal Studies; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000276:immunology / Q000821:virology*; D022783:Torque teno virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral