title: [Absorption mechanism of oxysophocarpine across Caco-2 cell monolayer mode].
abstract: OBJECTIVE
  To investigate the absorption mechanism of oxysophocarpine across Caco-2 cell monolayer model.
  
  
  METHODS
  The safety concentration of oxysophocarpine in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayers drug transport model was assigned to study the bi-direction transport mechanism of oxysophocarpine by evaluating the influent factors such as time, concentration, pH, P-gp inhibitor of verapamil, on its absorption characterization.
  
  
  RESULTS
  In the Caco-2 cell monolayer model, the transport volume was correlated positively with the time and concentration of oxysophocarpine, and affected by pH value. Verapamil had no influence on its transport since the transport of oxysophocarpine from apical (AP) to basolateral (BL) was similar to the transport from basolateral to apical.
  
  
  CONCLUSIONS
  The intestinal absorption mechanism of oxysophocarpine was deduced as passive transference by Caco-2 cell monolayer model.
authors: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors: Zhiqiang
authors: Z
authors: Feng
authors: Zhiyong
authors: Z
authors: Xie
authors: Qiongfeng
authors: Q
authors: Liao
authors: Xiaojing
authors: X
authors: Tan
authors: Meicun
authors: M
authors: Yao
authors: Lei
authors: L
authors: Zhang
mesh_terms: D000470:Alkaloids / Q000493:pharmacokinetics*; D001692:Biological Transport; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects*; D008954:Models, Biological; D000093842:Matrines
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C046246:oxysophocarpine; D000093842:Matrines