title: A study on apoptosis inducing effects of UVB irradiation in Pseudomonas aeruginosa.
abstract: BACKGROUND
  Pseudomonas aeruginosa is an important bacterial pathogen which causes different infectious diseases such as wound and skin lesion infections. The main goal of this study was to induce eventual apoptotic reactions in ultraviolet-exposed colonies of Pseudomonas aeruginosa.
  
  
  METHODS
  The colonies of Pseudomonas aeruginosa were irradiated by UVB light; then, the DNA molecules of control and UVB-exposed colonies were extracted. Eventually, the extracted DNA molecules mixed in loading dye were run in 1% agarose gel containing ethidium bromide.
  
  
  RESULTS
  No unusual pattern like DNA laddering bands or smear, were detected upon the 1% agarose gel.
  
  
  CONCLUSIONS
  Through the applied protocol in this survey, the UVB radiation is not able to trigger apoptosis pathway in UV light exposed colonies of Pseudomonas aeruginosa. It seems that the cytoprotective property of Heat shock proteins inhibit the inducing effect of UVB light in irradiated colonies of Pseudomonas aeruginosa.
authors: Microbiology Department, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran--Iran.
authors: Payam
authors: P
authors: Behzadi
authors: Elham
authors: E
authors: Behzadi
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D011550:Pseudomonas aeruginosa / Q000528:radiation effects*; D014466:Ultraviolet Rays