title: Mucosal delivery of ACNPV baculovirus driving expression of the Gal-lectin LC3 fragment confers protection against amoebic liver abscess in hamster.
abstract: Mucosal vaccination against amoebiasis using the Gal-lectin of E. histolytica has been proposed as one of the leading strategies for controlling this human disease. However, most mucosal adjuvants used are toxic and the identification of safe delivery systems is necessary. Here, we evaluate the potential of a recombinant Autographa californica baculovirus driving the expression of the LC3 fragment of the Gal-lectin to confer protection against amoebic liver abscess (ALA) in hamsters following oral or nasal immunization. Hamsters immunized by oral route showed complete absence (57.9%) or partial development (21%) of ALA, resulting in some protection in 78.9% of animals when compared with the wild type baculovirus and sham control groups. In contrast, nasal immunization conferred only 21% of protection efficacy. Levels of ALA protection showed lineal correlation with the development of an anti-amoebic cellular immune response evaluated in spleens, but not with the induction of seric IgG anti-amoeba antibodies. These results suggest that baculovirus driving the expression of E. histolytica vaccine candidate antigens is useful for inducing protective cellular and humoral immune responses following oral immunization, and therefore it could be used as a system for mucosal delivery of an anti-amoebic vaccine.
authors: Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. A.P. 70228, México D.F., México.
authors: D M
authors: DM
authors: Meneses-Ruiz
authors: J P
authors: JP
authors: Laclette
authors: H
authors: H
authors: Aguilar-Díaz
authors: J
authors: J
authors: Hernández-Ruiz
authors: A
authors: A
authors: Luz-Madrigal
authors: A
authors: A
authors: Sampieri
authors: L
authors: L
authors: Vaca
authors: J C
authors: JC
authors: Carrero
mesh_terms: D000562:Amebiasis / Q000276:immunology / Q000517:prevention & control; D000656:Amoeba / Q000276:immunology / Q000472:pathogenicity; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000517:prevention & control*; D018411:Spodoptera
chemical_list: D000953:Antigens, Protozoan