title: Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydrolase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg.
abstract: A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.
authors: Departamento de Bioquímica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brasil.
authors: Rita Gabriela Pedrosa Ribeiro
authors: RG
authors: Mendes
authors: Michélia Antônia do Nascimento
authors: MA
authors: Gusmão
authors: Ana Carolina Ribeiro Gomes
authors: AC
authors: Maia
authors: Michelle de Lima
authors: Mde L
authors: Detoni
authors: Gabriane Nascimento
authors: GN
authors: Porcino
authors: Thais Vieira
authors: TV
authors: Soares
authors: Maria Aparecida
authors: MA
authors: Juliano
authors: Luiz
authors: L
authors: Juliano
authors: Paulo Marcos Zech
authors: PM
authors: Coelho
authors: Henrique Leonel
authors: HL
authors: Lenzi
authors: Priscila
authors: P
authors: Faria-Pinto
authors: Eveline Gomes
authors: EG
authors: Vasconcelos
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D001081:Apyrase / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D004527:Egg Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000201:enzymology / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D004527:Egg Proteins; D001081:Apyrase