title: Enhanced bacteremia in human factor H transgenic rats infected by Neisseria meningitidis.
abstract: Neisseria meningitidis binds the complement downregulating protein, factor H (fH), which enables the organism to evade host defenses. Two fH ligands, fHbp and NspA, are known to bind specifically to human fH. We developed a human fH transgenic infant rat model to investigate the effect of human fH on meningococcal bacteremia. At 18 h after intraperitoneal challenge with 560 CFU of group B strain H44/76, all 19 human fH-positive rats had positive blood cultures compared to 0 of 7 human fH-negative control littermates (P < 0.0001). Human fH-positive infant rats also developed bacteremia after challenge with isogenic mutants of H44/76 in which genes encoding fHbp and NspA (ΔfHbp ΔNspA mutant) or the lipooligosaccharide sialyltransferase (Δlst mutant) had been inactivated. A fully encapsulated ΔfHbp ΔNspA Δlst mutant unable to sialylate lipooligosaccharide or bind human fH via the known fH ligands did not cause bacteremia, which argued against global susceptibility to bacteremia resulting from random integration of the transgene into the rat genome. In vitro, the wild-type and ΔfHbp ΔNspA mutant strains were killed by as little as 20% wild-type infant rat serum. The addition of 3 μg of human fH/ml permitted survival of the wild-type strain in up to 60% infant rat serum, whereas ≥33 μg of human fH/ml was required to rescue the ΔfHbp ΔNspA mutant. The ability of meningococci lacking expression of fHbp and NspA to cause invasive disease in human fH transgenic rats and to survive in wild-type infant rat serum supplemented with human fH indicates an additional human fH-dependent mechanism of evasion of innate immunity.
authors: Center for Immunobiology and Vaccine Development, Children's Hospital Oakland Research Institute, Oakland, California, USA.
authors: David M
authors: DM
authors: Vu
authors: Jutamas
authors: J
authors: Shaughnessy
authors: Lisa A
authors: LA
authors: Lewis
authors: Sanjay
authors: S
authors: Ram
authors: Peter A
authors: PA
authors: Rice
authors: Dan M
authors: DM
authors: Granoff
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D016470:Bacteremia / Q000276:immunology* / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D017242:Complement Factor H / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D008589:Meningococcal Infections / Q000235:genetics / Q000276:immunology*; D009345:Neisseria meningitidis / Q000235:genetics*; D051381:Rats
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C105506:NspA protein, Neisseria; C484699:factor H-binding protein, Neisseria meningitidis; D017242:Complement Factor H