title: Hypothalamic Ahi1 mediates feeding behavior through interaction with 5-HT2C receptor.
abstract: It is indicated that there are important molecules interacting with brain nervous systems to regulate feeding and energy balance by influencing the signaling pathways of these systems, but relatively few of the critical players have been identified. In the present study, we provide the evidence for the role of Abelson helper integration site 1 (Ahi1) protein as a mediator of feeding behavior through interaction with serotonin receptor 2C (5-HT(2C)R), known for its critical role in feeding and appetite control. First, we demonstrated the co-localization and interaction between hypothalamic Ahi1 and 5-HT(2C)R. Ahi1 promoted the degradation of 5-HT(2C)R through the lysosomal pathway. Then, we investigated the effects of fasting on the expression of hypothalamic Ahi1 and 5-HT(2C)R. Fasting resulted in an increased Ahi1 expression and a concomitant decreased expression of 5-HT(2C)R. Knockdown of hypothalamic Ahi1 led to a concomitant increased expression of 5-HT(2C)R and a decrease of food intake and body weight. Last, we found that Ahi1 could regulate the expression of neuropeptide Y and proopiomelanocortin. Taken together, our results indicate that Ahi1 mediates feeding behavior by interacting with 5-HT(2C)R to modulate the serotonin signaling pathway.
authors: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors: Hao
authors: H
authors: Wang
authors: Zhenbo
authors: Z
authors: Huang
authors: Liansha
authors: L
authors: Huang
authors: Shaona
authors: S
authors: Niu
authors: Xiurong
authors: X
authors: Rao
authors: Jing
authors: J
authors: Xu
authors: Hui
authors: H
authors: Kong
authors: Jianzhong
authors: J
authors: Yang
authors: Chuan
authors: C
authors: Yang
authors: Donghai
authors: D
authors: Wu
authors: Shihua
authors: S
authors: Li
authors: Xiao-Jiang
authors: XJ
authors: Li
authors: Tonghua
authors: T
authors: Liu
authors: Guoqing
authors: G
authors: Sheng
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001069:Appetite Regulation / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009478:Neuropeptide Y / Q000096:biosynthesis; D059748:Proteolysis; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D044405:Receptor, Serotonin, 5-HT2C / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C465818:Ahi1 protein, mouse; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D011518:Proto-Oncogene Proteins; D044405:Receptor, Serotonin, 5-HT2C; D012701:Serotonin