title: Candida albicans strain-dependent modulation of pro-inflammatory cytokine release by in vitro oral and vaginal mucosal models.
abstract: Candida albicans is a commensal organism at several sites and is a versatile, opportunistic pathogen. The underlying factors of pathogen and host associated with commensalism and pathogenicity in C. albicans are complex and their importance is largely unknown. We aimed to study the responses of oral epithelial (OEM) and vaginal epithelial models (VEM) to infection by oral and vaginal C. albicans strains to obtain evidence of inter-strain differences in pathogenicity and of site-specificity. Following inoculation of models, proinflammatory cytokines IL-1α, IL-1β, IL-6, IL-8 and prostaglandin E2 (PGE2) release were monitored and histological staining undertaken. Striking differences in strain behaviour and epithelial responses were observed. IL-1α, IL-1β and IL-8 release were significantly increased from the OEM in response to denture stomatitis strain NCYC 1467. Increased IL-8 release also followed infection of the OEM with both vaginal strains. Overall the VEM was relatively unresponsive to infection with either oral or vaginal strains under these conditions. Adherence and hyphal development were observed for all strains on both models although extensive, uniform tissue penetration was seen only with stomatitis strain NCYC 1467 on the OEM. Candidal strains were assayed for phospholipase (PL) and secreted aspartyl proteinase (SAP) activities where phospholipase (PL) activity was highest for strain NCYC 1467 although highest SAP activity was observed for vaginal strain NCPF 8112 in this assay. This is the first study to concurrently investigate cytokine production from oral and epithelial models using candidal strains originating from these respective mucosal sites from healthy and disease states. These data demonstrate significant differences in inflammatory responses of host epithelia to individual C. albicans strains.
authors: Institute of Dentistry, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, Turner Street, London E1 2AD, United Kingdom.
authors: Robert A
authors: RA
authors: Whiley
authors: Alan T
authors: AT
authors: Cruchley
authors: Carelyn
authors: C
authors: Gore
authors: Eleni
authors: E
authors: Hagi-Pavli
mesh_terms: D000268:Adhesiveness; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis; D002176:Candida albicans / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D004848:Epithelium / Q000382:microbiology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008954:Models, Biological; D009061:Mouth Mucosa / Q000382:microbiology*; D010740:Phospholipases / Q000096:biosynthesis; D014621:Vagina / Q000382:microbiology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D010740:Phospholipases; D016282:Aspartic Acid Endopeptidases; C086390:SAP1 protein, Candida albicans; D015232:Dinoprostone