title: A BioDVD media with multilayered structure is suitable for analyzing biomolecular interactions.
abstract: A biomolecular interactive analysis with antibody-antigen and aptamer-protein was evaluated on Au-over layers deposited on the BioDVD surface. BioDVD consists of multilayered structures with Au layer on the top and it detects analytes by monitoring the changes in reflected light intensity due to analyte adsorption to the sensor surface, on which functional biomolecules are immobilized to bind specifically to the analytes. The BioDVD sensing instrument is based on a commercial digital versatile disc system, which allows the instrument to be small and inexpensive. The BioDVD platform can be fabricated utilizing mass production techniques with additional functional phase change layers that can serve both to enhance sensitivity by optimization of the interferometric cavity optical properties and also as a possible medium for the storage of test related information.
authors: Nanoelectronics Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibraki 305-8562, Japan.
authors: Subash C B
authors: SC
authors: Gopinath
authors: Penmetcha K R
authors: PK
authors: Kumar
authors: Junji
authors: J
authors: Tominaga
mesh_terms: D000327:Adsorption; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D052158:Aptamers, Peptide / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D016250:Compact Disks; D004867:Equipment Design; D054303:HIV Reverse Transcriptase / Q000032:analysis / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis / Q000378:metabolism; D055767:Immobilized Proteins / Q000032:analysis / Q000378:metabolism; D007118:Immunoassay / Q000295:instrumentation; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology; D025941:Protein Interaction Mapping / Q000295:instrumentation; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D055767:Immobilized Proteins; D054303:HIV Reverse Transcriptase