title: A fully human in vitro capsular bag model to permit intraocular lens evaluation.
abstract: OBJECTIVE
  To establish a fully human in vitro culture model with which to test the putative effects of intraocular lens (IOL) designs in preventing posterior capsule opacification (PCO) after cataract surgery.
  
  
  METHODS
  A sham cataract operation was performed to prepare human capsular bags from donor lenses. In one capsular bag of a donor pair, an intraocular lens (PMMA round-edge IOL or acrylic IOL) was implanted while the other capsular bag remained aphakic. Bags were transferred to a Petri dish and secured anterior-face down using entomological pins. Capsular bags were maintained in Eagle's minimum essential medium supplemented with 2% human serum and 10 ng/mL TGF-β to drive growth and matrix contraction.
  
  
  RESULTS
  In the absence of an IOL, cells appeared within the central posterior capsule at 4.38 ± 0.26 days, whereas in the presence of a PMMA round-edge IOL or an acrylic IOL they appeared at 8 ± 0.41 days and 11 ± 0.7 days, respectively. Immunocytochemical analysis showed an accumulation of cells at the edge of the acrylic IOL and a less evident accumulation with the PMMA round-edge IOL. Moreover, matrix contraction was more prominent in the absence of an IOL but was still apparent, to a lesser degree, in the presence of a PMMA round-edge IOL. The acrylic IOL greatly suppressed matrix contraction.
  
  
  CONCLUSIONS
  The authors have developed a fully human in vitro capsular bag system that relates well to clinical observations and permits the testing of novel intraocular lenses.
authors: School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
authors: Lucy J
authors: LJ
authors: Dawes
authors: Christopher D
authors: CD
authors: Illingworth
authors: I Michael
authors: IM
authors: Wormstone
mesh_terms: D000180:Acrylic Resins; D000199:Actins / Q000378:metabolism; D058442:Capsule Opacification / Q000517:prevention & control*; D049109:Cell Proliferation; D002843:Chromatin / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D019654:Lens Implantation, Intraocular; D007910:Lenses, Intraocular; D008875:Middle Aged; D008954:Models, Biological; D009924:Organ Culture Techniques; D019904:Polymethyl Methacrylate; D057852:Posterior Capsule of the Lens / Q000473:pathology*; D011474:Prosthesis Design; D014019:Tissue Donors; D014746:Vimentin / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D000199:Actins; D002843:Chromatin; D014746:Vimentin; D019904:Polymethyl Methacrylate