title: Stress clamp experiments on multicellular tumor spheroids.
abstract: The precise role of the microenvironment on tumor growth is poorly understood. Whereas the tumor is in constant competition with the surrounding tissue, little is known about the mechanics of this interaction. Using a novel experimental procedure, we study quantitatively the effect of an applied mechanical stress on the long-term growth of a spheroid cell aggregate. We observe that a stress of 10 kPa is sufficient to drastically reduce growth by inhibition of cell proliferation mainly in the core of the spheroid. We compare the results to a simple numerical model developed to describe the role of mechanics in cancer progression.
authors: UMR 168, Institut Curie, Centre de Recherche, 26 rue d'Ulm 75005 Paris, France.
authors: Fabien
authors: F
authors: Montel
authors: Morgan
authors: M
authors: Delarue
authors: Jens
authors: J
authors: Elgeti
authors: Laurent
authors: L
authors: Malaquin
authors: Markus
authors: M
authors: Basan
authors: Thomas
authors: T
authors: Risler
authors: Bernard
authors: B
authors: Cabane
authors: Danijela
authors: D
authors: Vignjevic
authors: Jacques
authors: J
authors: Prost
authors: Giovanni
authors: G
authors: Cappello
authors: Jean-François
authors: JF
authors: Joanny
mesh_terms: D017209:Apoptosis; D049109:Cell Proliferation; D003198:Computer Simulation; D006801:Humans; D008954:Models, Biological; D018874:Spheroids, Cellular / Q000473:pathology*; D013312:Stress, Physiological; D014407:Tumor Cells, Cultured