title: Calcineurin subunit B upregulates β-interferon production by phosphorylation of interferon regulatory factor 3 via Toll-like receptor 4.
abstract: Toll-like receptors (TLRs) function as pattern-reorganization receptors in mammals and play an essential role in innate immunity. After recognizing certain ligands, TLRs activate a cascade of signal pathways to establish a guard environment. For the first time, we report that in vitro treatment with recombinant calcineurin subunit B (CNB) upregulated several TLR-related genes. Calcineurin subunit B interacted with the ectodomain of TLR4 in vitro. On further investigation, phosphorylation of interferon regulatory factor 3 and degradation of IκB-α were observed by CNB stimulation. In addition to pro-inflammatory cytokines, transcription and production of β-interferon were also enhanced after CNB stimulation. Thus, CNB could be further explored as a cancer and virus immunotherapy drug.
authors: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, Beijing, China.
authors: Xinyu
authors: X
authors: Wang
authors: Li
authors: L
authors: Li
authors: Yi
authors: Y
authors: Huang
authors: Qun
authors: Q
authors: Wei
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019703:Calcineurin / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology; D050838:Interferon Regulatory Factor-3 / Q000276:immunology / Q000378:metabolism*; D016899:Interferon-beta / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D060888:Real-Time Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D050838:Interferon Regulatory Factor-3; D051197:Toll-Like Receptor 4; D016899:Interferon-beta; D019703:Calcineurin