title: Determination of taurine in biological samples by high-performance liquid chromatography using 4-fluoro-7-nitrobenzofurazan as a derivatizing agent.
abstract: OBJECTIVE
  A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples, including plasma, brain, and liver.
  
  
  METHODS
  The optimum derivatization reaction temperature was 70 °C, and at this temperature the reaction was complete within 3 min. The derivatized taurine was separated using phosphate buffer (0.02 mol/L, pH 6.0):acetonitrile (84:16, v/v) as the mobile phase at a flow rate of 1.0 mL/min, and a column temperature of 25 °C. The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.
  
  
  RESULTS
  The intra- and the inter-day coefficients of variation for the method were 5.3% and 7.7%, respectively. The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.
  
  
  CONCLUSIONS
  This method can be used to determine the taurine contents in plasma, brain, and liver from normal rats and human plasma.
authors: Beijing Daxing Center for Disease Control and Prevention, Beijing 102600, China.
authors: XiFeng
authors: X
authors: Wang
authors: DeFeng
authors: D
authors: Chi
authors: GuanMin
authors: G
authors: Su
authors: Lin
authors: L
authors: Li
authors: LiHua
authors: L
authors: Shao
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013654:Taurine / Q000032:analysis* / Q000097:blood; D013696:Temperature
chemical_list: D000097:Acetonitriles; D005456:Fluorescent Dyes; D012997:Solvents; D013654:Taurine; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D009327:4-Chloro-7-nitrobenzofurazan; C032159:acetonitrile