title: Determination of cyclobenzaprine in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry and its application in a pharmacokinetic study.
abstract: A sensitive, rapid and simple liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS) method was developed for the quantitative determination of cyclobenzaprine in human plasma, to study the pharmacokinetic behavior of cyclobenzaprine capsule in healthy Chinese volunteers. With escitalopram as the internal standard (IS), sample pretreatment involved a one-step liquid-liquid extraction using saturated sodium carbonate solution and hexane-diethyl ether (3:1, v/v). The separation was performed on an Ultimate XB-CN column (150 × 2.1 mm, 5 µm). Isocratic elution was applied using acetonitrile-water (40:60, v/v) containing 10 m M ammonium acetate and 0.1% formic acid. The detection was carried out on a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode via electrospray ionization. The ion-pairs including m/z 276.2-216.2 for cyclobenzaprine and m/z 325.2-109.1 for IS were used for monitoring. Linear calibration curves were obtained over the range of 0.049-29.81 ng/mL with the lower limit of quantification at 0.049 ng/mL. The intra- and inter-day precision showed ≤ 6.5% relative standard deviation. The established method laid the groundwork for follow-up studies and provided basis for the clinical administration of cyclobenzaprine.
authors: College of Pharmacy, Tongji Medical Colllege, Huazhong University of Science and Technology, Wuhan 430030, China.
authors: Yi
authors: Y
authors: Xiang
authors: Liping
authors: L
authors: Zhou
authors: Zhenyu
authors: Z
authors: Qian
authors: Kelong
authors: K
authors: Peng
authors: Dongyan
authors: D
authors: Li
authors: Xiaomei
authors: X
authors: Chen
authors: Hongliang
authors: H
authors: Jiang
authors: Heng
authors: H
authors: Zheng
mesh_terms: D000639:Amitriptyline / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D015283:Citalopram; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015283:Citalopram; D000639:Amitriptyline; C004704:cyclobenzaprine