title: A single cluster of coregulated genes encodes the biosynthesis of the mycotoxins roquefortine C and meleagrin in Penicillium chrysogenum.
abstract: A single gene cluster of Penicillium chrysogenum contains genes involved in the biosynthesis and secretion of the mycotoxins roquefortine C and meleagrin. Five of these genes have been silenced by RNAi. Pc21g15480 (rds) encodes a nonribosomal cyclodipeptide synthetase for the biosynthesis of both roquefortine C and meleagrin. Pc21g15430 (rpt) encodes a prenyltransferase also required for the biosynthesis of both mycotoxins. Silencing of Pc21g15460 or Pc21g15470 led to a decrease in roquefortine C and meleagrin, whereas silencing of the methyltransferase gene (Pc21g15440; gmt) resulted in accumulation of glandicolin B, indicating that this enzyme catalyzes the conversion of glandicolin B to meleagrin. All these genes are transcriptionally coregulated. Our results prove that roquefortine C and meleagrin derive from a single pathway.
authors: Instituto de Biotecnología de León (INBIOTEC), Parque Científico de La Granja, Avda. Real, 1, 24006 León, Spain.
authors: Carlos
authors: C
authors: García-Estrada
authors: Ricardo V
authors: RV
authors: Ullán
authors: Silvia M
authors: SM
authors: Albillos
authors: María Ángeles
authors: MÁ
authors: Fernández-Bodega
authors: Pawel
authors: P
authors: Durek
authors: Hans
authors: H
authors: von Döhren
authors: Juan F
authors: JF
authors: Martín
mesh_terms: D001665:Binding Sites; D055162:Biocatalysis; D004122:Dimethylallyltranstransferase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000378:metabolism; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D008780:Methyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D009183:Mycotoxins / Q000096:biosynthesis; D010059:Ovomucin / Q000096:biosynthesis*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010408:Penicillium chrysogenum / Q000235:genetics* / Q000378:metabolism; D010879:Piperazines / Q000737:chemistry / Q000378:metabolism; D017368:Protein Prenylation; D017434:Protein Structure, Tertiary; D034622:RNA Interference
chemical_list: D005656:Fungal Proteins; D006576:Heterocyclic Compounds, 4 or More Rings; D007211:Indoles; D009183:Mycotoxins; D010879:Piperazines; D010059:Ovomucin; C060672:meleagrin; C012536:roquefortine; D010088:Oxidoreductases; D008780:Methyltransferases; D004122:Dimethylallyltranstransferase