title: Seeding a human tendon matrix with bone marrow aspirates compared to previously isolated hBMSCs--an in vitro study.
abstract: Injuries of tendons and ligaments give rise to significant morbidity. Tissue engineering offers promising treatment concepts such as seeding a scaffold with human bone marrow stem cells (hBMSCs) to create high-quality tendon replacement tissue. HBMSCs are usually isolated and cultured prior to seeding. Studies evaluating if previous isolation is superior to seeding with bone marrow aspirates have not been published yet. The aim of this study was to compare these two seeding methods in terms of cell viability, proliferation and differentiation. HBMSCs were harvested from the iliac crest during routine trauma surgery. In group A the scaffold (human achilles tendons) was seeded with bone marrow aspirates, while in group B hBMSCs were isolated, harvested and then seeded. Constructs were stimulated in perfusion bioreactors according to established protocols. Mean cell proliferation was significantly increased (p< 0.05) on tendons seeded with bone marrow aspirates. Cell viability, the concentration of alkaline phosphatase in the perfused media and the synthesis of procollagen - III - polypeptide (PIIIP) were not significantly different when comparing the two groups. The synthesis of procollagen-I-polypeptide (PIP) was significantly increased on tendons seeded with previously isolated hBMSCs p < 0.05). The results indicate that seeding a human tendon matrix scaffold with bone marrow aspirates may be equal to seeding with previously isolated hBMSCs. This new seeding method could facilitate and speed up the tissue engineering process.
authors: Department of Trauma Surgery, Hannover Medical School, Hannover, Germany. maxbroese@hotmail.com
authors: Maximilian
authors: M
authors: Broese
authors: Ionel
authors: I
authors: Toma
authors: Carl
authors: C
authors: Haasper
authors: Annika
authors: A
authors: Simon
authors: Maximilian
authors: M
authors: Petri
authors: Stefan
authors: S
authors: Budde
authors: Michael
authors: M
authors: Wehmeier
authors: Christian
authors: C
authors: Krettek
authors: Michael
authors: M
authors: Jagodzinski
mesh_terms: D000125:Achilles Tendon / Q000166:cytology* / Q000502:physiology; D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008875:Middle Aged; D011347:Procollagen / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D036341:Intercellular Signaling Peptides and Proteins; D011347:Procollagen; D000469:Alkaline Phosphatase