title: [Strontium ranelate promotes osteogenic differentiation of rat bone marrow mesenchymal stem cells by increasing bone morphogenetic protein-7 expression].
abstract: OBJECTIVE
  To explore the role of bone morphogenetic protein-7 (BMP-7) in strontium ranelate (Sr)-induced osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
  
  
  METHODS
  BMSCs were isolated from 4-week-old rats and cultured in vitro. The third or fourth passages of BMSCs were examined using alkaline phosphatase kit for changes in ALP activity in response to treatment with different concentrations of Sr. Calcium nodules in the induced cells were detected using alizarin red staining, and the cellular BMP-7 expression was detected by Western blotting.
  
  
  RESULTS
  Within the concentration range of 0.1-3.0 mmol/L, Sr dose-dependently increased ALP activity in rat BMSCs. ALP activity reached the highest level after treatment with 3 mmol/L Sr, which also significantly promoted the formation of calcium nodules. Within the range of 0.1-3.0 mmol/L, Sr dose-dependently enhanced the expression of BMP-7, and its peak expression occurred following 3 mmol/L Sr treatment. Noggin (100 ng/ml), an inhibitor of BMP-7, obviously suppressed Sr-induced over-expression of BMP-7 and reduced ALP activity and calcium nodule formation in the BMSCs.
  
  
  CONCLUSIONS
  Sr promotes osteogenic differentiation of rat BMSCs by increasing the expression of BMP-7.
authors: Southern Medical University, Guangzhou 510515, China. lizheng19850125@163.com
authors: Zheng
authors: Z
authors: Li
authors: Yang
authors: Y
authors: Wang
authors: Xiao-na
authors: XN
authors: Wang
authors: Ai-ping
authors: AP
authors: Lan
authors: Wen
authors: W
authors: Wu
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology*; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009942:Organometallic Compounds / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: C525821:Bmp7 protein, rat; D050071:Bone Density Conservation Agents; D055419:Bone Morphogenetic Protein 7; D009942:Organometallic Compounds; D013876:Thiophenes; C081587:strontium ranelate