title: [Role of CD44(+)/ESA(+) in sorting colonic cancer stem cells].
abstract: OBJECTIVE
  To isolate CD133(+)/CD44(+)/ESA(+) subsets cells from SW480 colon cancer cells, and to observe the tumor formation.
  
  
  METHODS
  CD133(+)/CD44(+)/ESA(+) subsets cells, CD133(-)/CD44(+)/ESA(+) subsets cells and CD133(-)/CD44(-)/ESA(-) subsets cell were sorted by flow cytometry from SW480 colon cancer cells, then three subsets were separately inoculated in five NOD/SCID mice and the growth rates were calculated.
  
  
  RESULTS
  The proportion of CD133(-)/CD44(-)/ESA(-), CD133(-)/CD44(+)/ESA(+) and CD133(+)/CD44(+)/ESA(+) subsets cells in SW480 cells were (86.38±10.23)%,(1.26±0.28)% and(0.38±0.07)%. After inoculation, tumor nodules could be formed three days later in CD133(+)/CD44(+)/ESA(+) group, and they could be formed 9 days later in CD133(-)/CD44(+)/ESA(+) group, while they could be formed 15 days later in CD133(-)/CD44(-)/ESA(-) group. Eighteen days later, tumor sizes in three groups were(13.82±5.04) mm(3), (9.25±4.57) mm(3) and (4.76±3.92) mm(3) respectively, and the differences were statistically significant(P<0.05).
  
  
  CONCLUSIONS
  ESA(+)-CD44(+) is one of the surface markers for colonic cancer stem cells, and CD133(+)-CD44(+)-ESA(+) cells are SW480-like cancer stem cells.
authors: Department of Cancer Surgery, Fujian Medical University, Fuzhou 350001, China. xuefangqingsl@sina.com
authors: Fang-qin
authors: FQ
authors: Xue
authors: Guo-hua
authors: GH
authors: Yang
authors: Ruo-lei
authors: RL
authors: Huang
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000166:cytology*; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D063268:Sialic Acid Binding Ig-like Lectin 3