title: Lack of microsatellite instability in squamous cell vulvar carcinoma.
abstract: Mutator phenotypes with microsatellite instability (MSI) are observed in a subset of solid tumors. The objective of our study was to investigate the occurrence of MSI in vulvar squamous cell carcinoma (VSCC) and a possible relation between MSI and the presence of human papilloma virus (HPV). DNA samples from 44 tissue specimens of the primary VSCC as well as from six metastatic lymph node samples were analysed and compared with matched reference DNA from blood samples. The MSI status was examined by polymerase chain reaction (PCR) using the Bethesda panel of five microsatellite markers. PCR products were analysed by fluorescent capillary electrophoresis. No microsatellite instability was detected in tumor samples or in metastatic lymph nodes from any of the VSCC patients examined. Microsatellite instability seems not to play a major role in the carcinogenesis of VSCC and is probably not associated with the HPV-related genetic background of this neoplasm.
authors: Department Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Centre, Warsaw, Poland.
authors: Mateusz
authors: M
authors: Bujko
authors: Magdalena
authors: M
authors: Kowalewska
authors: Renata
authors: R
authors: Zub
authors: Jakub
authors: J
authors: Radziszewski
authors: Mariusz
authors: M
authors: Bidzinski
authors: Janusz A
authors: JA
authors: Siedlecki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D008207:Lymphatic Metastasis; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D014846:Vulvar Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers