title: Screening for in vitro metabolites of Abelmoschus manihot extract in intestinal bacteria by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
abstract: Abelmoschus manihot has drawn much attention recently due to its potential beneficial health effects after oral administration. However, the metabolic fate of A. manihot in intestinal flora is not well understood. In this paper, we describe a strategy using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) with automated data analysis software (MetaboLynx™) for fast analysis of the metabolic profile of flavonoids from A. manihot in intestinal flora. The human and rat incubated samples collected 72 h in the anaerobic incubator were analyzed by UPLC-Q-TOF MS within 10 min. A total of 14 metabolites were identified in human and rat incubated solution compared with blank samples. The results indicated that hydrolysis, hydroxylation and acetylation were the major metabolic pathways of flavonoids in A. manihot extract in vitro. MS(E) was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the fast structural characterization of metabolites. This work demonstrated the potential of the UPLC-Q-TOF MS approach using Metabolynx for fast and automated identification of metabolites of natural product in intestinal flora.
authors: Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors: Caifu
authors: C
authors: Xue
authors: Shu
authors: S
authors: Jiang
authors: Jianming
authors: J
authors: Guo
authors: Dawei
authors: D
authors: Qian
authors: Jin-ao
authors: JA
authors: Duan
authors: Erxin
authors: E
authors: Shang
mesh_terms: D028483:Abelmoschus / Q000737:chemistry*; D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D006900:Hydroxylation; D007422:Intestines / Q000382:microbiology*; D008297:Male; D013058:Mass Spectrometry; D008745:Methylation; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids