title: Simultaneous analytical method for urinary metabolites of organophosphorus compounds and moth repellents in general population.
abstract: An analytical method was developed for simultaneous measurement of urinary metabolites in the general population exposed to organophosphorus compounds (insecticides, flame retardants and plasticizers) and moth repellents used in Japanese households. Fifteen metabolites, dimethylphosphate, dimethylthiophosphate, diethylphosphate, diethylthiophosphate, di-n-butylphosphate, diphenylphosphate, bis(2-ethylhexyl)phosphate, 2-isopropyl-6-methyl-4-pyrimidinol, 3,5,6-trichloro-2-pyridinol, 3-methyl-4-(methylthio)phenol, 3-methyl-4-nitrophenol, 2,4-dichlorophenol, 2,5-dichlorophenol, 1-naphthol and 2-naphthol, were extracted from hydrolyzed urine by using a sorbent (hydroxylated polystyrene-divinylbenzene copolymers), and then desorbed with methylacetate and acetonitrile, concentrated, and after transformation to their tert-butyldimethylsilyl derivatives, analyzed by gas chromatography/mass spectrometry in the electron impact ionization mode. They could be determined accurately and precisely (quantification limits: 0.8-4 μg/l). The collected urine samples could be stored for up to 1 month at -20°C in a freezer.
authors: Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka, Japan. tsyosida@iph.pref.osaka.jp
authors: Toshiaki
authors: T
authors: Yoshida
authors: Jin
authors: J
authors: Yoshida
mesh_terms: D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000302:isolation & purification; D000818:Animals; D004355:Drug Stability; D004781:Environmental Exposure; D005411:Flame Retardants / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007302:Insect Repellents / Q000652:urine*; D007306:Insecticides / Q000652:urine; D007564:Japan; D016014:Linear Models; D008875:Middle Aged; D009036:Moths; D009943:Organophosphorus Compounds / Q000302:isolation & purification / Q000652:urine*; D010968:Plasticizers / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000393:Air Pollutants; D005411:Flame Retardants; D007302:Insect Repellents; D007306:Insecticides; D009943:Organophosphorus Compounds; D010968:Plasticizers