title: Comprehensive proteomic profiling of aldehyde dehydrogenases in lung adenocarcinoma cell lines.
abstract: We have explored the potential of proteomic profiling to contribute to the delineation of the range of expression and subcellular localization of aldehyde dehydrogenases (ALDHs) in lung adenocarcinoma. In-depth quantitative proteomics was applied to 40 lung adenocarcinoma cell lines resulting in the identification of the known members of the ALDH family. Substantial heterogeneity in the level and occurrence of ALDHs in total lysates and on the cell surface and in their release into the culture media was observed based on mass spectrometry counts. A distinct pattern of expression of ALDHs was observed in cells exhibiting epithelial features relative to cells exhibiting mesenchymal features. Strikingly elevated levels of ALDH1A1 were observed in two cell lines. We also report on the occurrence of an immune response to ALDH1A1 in lung cancer.
authors: Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5-C800, Seattle, WA 98109, USA.
authors: Qing
authors: Q
authors: Zhang
authors: Ayumu
authors: A
authors: Taguchi
authors: Mark
authors: M
authors: Schliekelman
authors: Chee-Hong
authors: CH
authors: Wong
authors: Alice
authors: A
authors: Chin
authors: Rork
authors: R
authors: Kuick
authors: David E
authors: DE
authors: Misek
authors: Samir
authors: S
authors: Hanash