title: Production of Streptoverticillium cinnamoneum transglutaminase and cinnamic acid by recombinant Streptomyces lividans cultured on biomass-derived carbon sources.
abstract: Transglutaminase from Streptoverticillium cinnamoneum (StvcMTG) was produced using recombinant Streptomyces lividans. When grown on glycerol and xylose as sole carbon sources, S. lividans/StvcMTG produced 360 and 530 mg of StvcMTG per liter, respectively. With starch and xylan, the strain produced 230 and 400mg of StvcMTG per liter, respectively. Recombinant S. lividans/encP, which expresses phenylalanine ammonia lyase from Streptomyces maritimus, produced 160 mg/L of cinnamic acid from cellulose. These results show that S. lividans can assimilate various carbon sources and produce useful compounds in desirable quantities.
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors: Shuhei
authors: S
authors: Noda
authors: Takaya
authors: T
authors: Miyazaki
authors: Tsutomu
authors: T
authors: Tanaka
authors: Chiaki
authors: C
authors: Ogino
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D002244:Carbon / Q000378:metabolism*; D002934:Cinnamates / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D002934:Cinnamates; C029010:cinnamic acid; D002244:Carbon; D011503:Transglutaminases