title: Bisphenol A exposure modifies methylation of imprinted genes in mouse oocytes via the estrogen receptor signaling pathway.
abstract: Bisphenol A (BPA), a synthetic additive used to harden polycarbonate plastics and epoxy resin, is ubiquitous in our everyday environment. Many studies have indicated detrimental effects of BPA on the mammalian reproductive abilities. This study is aimed to test the potential effects of BPA on methylation of imprinted genes during oocyte growth and meiotic maturation in CD-1 mice. Our results demonstrated that BPA exposure resulted in hypomethylation of imprinted gene Igf2r and Peg3 during oocyte growth, and enhanced estrogen receptor (ER) expression at the levels of mRNA and protein. The relationship between ER expression and imprinted gene hypomethylation was substantiated using an ER inhibitor, ICI182780. In addition, BPA promoted the primordial to primary follicle transition, thereby speeding up the depletion of the primordial follicle pool, and suppressed the meiotic maturation of oocytes because of abnormal spindle assembling in meiosis I. In conclusion, neonatal exposure to BPA inhibits methylation of imprinted genes during oogenesis via the ER signaling pathway in CD-1 mice.
authors: Laboratory of Germ Cell Biology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, Qingdao Agricultural University, Qingdao, China.
authors: Hu-He
authors: HH
authors: Chao
authors: Xi-Feng
authors: XF
authors: Zhang
authors: Bo
authors: B
authors: Chen
authors: Bo
authors: B
authors: Pan
authors: Lian-Jun
authors: LJ
authors: Zhang
authors: Lan
authors: L
authors: Li
authors: Xiao-Feng
authors: XF
authors: Sun
authors: Qing-Hua
authors: QH
authors: Shi
authors: Wei
authors: W
authors: Shen
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D019175:DNA Methylation / Q000187:drug effects* / Q000235:genetics; D005260:Female; D018392:Genomic Imprinting / Q000187:drug effects / Q000235:genetics*; D051741:Kruppel-Like Transcription Factors / Q000187:drug effects / Q000235:genetics*; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis / Q000187:drug effects / Q000235:genetics; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology; D010636:Phenols / Q000633:toxicity*; D017527:Receptor, IGF Type 2 / Q000187:drug effects / Q000235:genetics*; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D051741:Kruppel-Like Transcription Factors; D010636:Phenols; D017527:Receptor, IGF Type 2; D011960:Receptors, Estrogen; C097901:Peg3 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds