title: Assessment of infection with polyomaviruses BKV, JCV and SV40 in different groups of Cuban individuals.
abstract: We investigated the frequency of BKV, JCV and SV40 reactivation in three groups of Cuban patients by multiplex nested PCR assay of 40 paraffin-embedded colorectal neoplasm tissues, 113 urine samples, and 125 plasma samples from 27 transplant recipients, and cerebrospinal fluid (CSF) from 67 HIV-1-infected individuals with central nervous system (CNS) disorders. None of these polyomaviruses were detected in colorectal neoplasms. JCV DNA was detected in 2 of 67 patients (2.9%) with CNS disorders, but neither BKV nor SV40 was identified. BKV was found in urine from 38.5% and 28.6% of adult and pediatric transplant recipients, respectively. In adult renal transplant recipients, excretion of BKV in urine was significantly associated with episodes of acute rejection (p=0.012) and with excretion of HCMV in urine (p= 0.008). In Cuba, the polyomaviruses studied here could not be related to colorectal neoplasms, and JCV was rarely detected in CSFs of HIV-1-infected individuals, whilst BKV reactivation was found to occur frequently in organ transplant recipients.
authors: STD laboratory, Department of Virology, Institute of Tropical Medicine Pedro Kourí, Autopista Novia del Mediodía, Km 6 ½, La Lisa, Marianao 13, Havana City, Cuba. arielmr@ipk.sld.cu
authors: Pedro A
authors: PA
authors: Martínez
authors: Vivian
authors: V
authors: Kourí
authors: Gustavo
authors: G
authors: Cordero
authors: Consuelo
authors: C
authors: Correa
authors: Yudira
authors: Y
authors: Soto
authors: Virginia
authors: V
authors: Capó
authors: Licet
authors: L
authors: González
authors: Bienvenido
authors: B
authors: Grá
authors: Gregorio
authors: G
authors: Petirena
authors: César
authors: C
authors: Silvério
authors: Norma
authors: N
authors: Hondal
authors: Mabel
authors: M
authors: González
authors: Iliana
authors: I
authors: Alvarez
authors: Elvira
authors: E
authors: Dorticós
authors: Alberto
authors: A
authors: Arencibia
authors: Juan C
authors: JC
authors: Jaime
authors: José
authors: J
authors: Florin
authors: Lourdes
authors: L
authors: Pérez
authors: Diana P
authors: DP
authors: Duran
authors: Juan José
authors: JJ
authors: Marchena
authors: Luis
authors: L
authors: Solar
authors: Nancy
authors: N
authors: Cazorla
authors: Yardelys
authors: Y
authors: Pérez
authors: Yoan
authors: Y
authors: Alemán
authors: Lissette
authors: L
authors: Pérez
authors: Alina
authors: A
authors: Álvarez
mesh_terms: D000328:Adult; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003462:Cuba; D005260:Female; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000821:virology*; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014412:Tumor Virus Infections / Q000821:virology*; D055815:Young Adult