title: Pathogenicity and insect transmission of a begomovirus complex between tomato yellow leaf curl virus and Ageratum yellow vein betasatellite.
abstract: Tomato yellow leaf curl virus (TYLCV) and Ageratum yellow vein betasatellite (AYVB) are members of the genus Begomovirus (family Geminiviridae). TYLCV and AYVB have been found in Japan over the last 15 years, and are associated with tomato leaf curl and the tomato yellow leaf curl diseases (TYLCD). AYVB is also associated with some monopartite begomoviruses. We have cloned both TYLCV and AYVB and demonstrated that TYLCV can trans-replicate with AYVB in Nicotiana benthamiana and tomato plants. A mixed infection of TYLCV and AYVB induced more severe symptoms of upward leaf curl, stunting, vein thickening, and swelling compared with TYLCV infection alone. The symptoms induced by infection of AYVB included a rise in abnormal cell proliferation, and pigmentation around leaf vein tissues. This is the first study to show that a complex of TYLCV and AYVB can be transmitted by vector insects among tomato plants. These results indicate that TYLCV possesses the potential to induce severe TYLCD by associating with AYVB.
authors: Kurume Research Station, National Agricultural Research Center for Kyushu Okinawa Region, 1823 Mii, Kurume, Fukuoka 839-8503, Japan. sued@affrc.go.jp
authors: Shigenori
authors: S
authors: Ueda
authors: Masatoshi
authors: M
authors: Onuki
authors: Masataka
authors: M
authors: Yamashita
authors: Yoichi
authors: Y
authors: Yamato
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D003001:Cloning, Molecular; D004261:DNA Replication; D004276:DNA, Satellite / Q000235:genetics / Q000302:isolation & purification*; D007313:Insecta / Q000821:virology*; D007564:Japan; D018551:Solanum lycopersicum / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
chemical_list: D004276:DNA, Satellite