title: Rapid LC-MS/MS profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes.
abstract: Amino acids extracted from a biological matrix can be resolved and measured using a 6-min per sample method through high-performance liquid chromatography with a short C18 column and rapid gradient using the ion-pairing reagent perfluoroheptanoic acid. LC-tandem mass spectrometry with multiple reaction monitoring (MRM) transitions selective for each compound allows simultaneous quantification of the 20 proteinogenic amino acids and 5 metabolically related compounds. Distinct MRM transitions were also established for selective detection of the isomers leucine/isoleucine and threonine/homoserine.
authors: Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA.
authors: Liping
authors: L
authors: Gu
authors: A Daniel
authors: AD
authors: Jones
authors: Robert L
authors: RL
authors: Last
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055442:Metabolome; D010641:Phenotype; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012015:Reference Standards; D012984:Software; D012996:Solutions
chemical_list: D000596:Amino Acids; D011506:Proteins; D012996:Solutions