title: Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI).
abstract: • Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high-quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition. • Here, we present our current protocols for the detection of small molecules in cryodissected immature barley (Hordeum vulgare) grains and tobacco (Nicotiana tabacum) roots. • Examples of MALDI-MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described. • Finally, we discuss how MALDI-MSI can contribute to applied plant research.
authors: Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
authors: Manuela
authors: M
authors: Peukert
authors: Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
authors: Andrea
authors: A
authors: Matros
authors: Department of Plant Nutrition, Estación Experimental de Aula Dei (CSIC), Av. Montañana 1005, Campus de Aula Dei, E-50059 Zaragoza, Spain.
authors: Giuseppe
authors: G
authors: Lattanzio
authors: Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
authors: Stephanie
authors: S
authors: Kaspar
authors: Department of Plant Nutrition, Estación Experimental de Aula Dei (CSIC), Av. Montañana 1005, Campus de Aula Dei, E-50059 Zaragoza, Spain.
authors: Javier
authors: J
authors: Abadía
authors: Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
authors: Hans-Peter
authors: HP
authors: Mock
mesh_terms: D016616:Cryoultramicrotomy; D001467:Hordeum / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013223:Statistics as Topic; D014026:Nicotiana / Q000378:metabolism*