title: Transglutaminase-mediated modification of ovomucoid: effects on its trypsin inhibitory activity and antigenic properties.
abstract: Hen egg can cause food hypersensitivity in infants and young children, and ovomucoid is the most allergenic factor among proteins contained in egg white. Since proteinase treatment, a well-recognized strategy in reducing food allergenicity, is ineffective when applied to ovomucoid because of its ability to act as trypsin inhibitor, we investigated the possibility of reducing the ovomucoid antiprotease activity and antigenic properties by covalently modifying its structure. The present paper reports data showing the ability of the Gln115 residue of ovomucoid to act as an acyl donor substrate for the enzyme transglutaminase and, as a consequence, to give rise to a covalent monodansylcadaverine conjugate of the protein in the presence of both enzyme and the diamine dansylated derivative. Moreover, we demonstrated that the obtained structural modification of ovomucoid significantly reduced the capability of the protein to inhibit trypsin activity, also having impact on its anti-ovomucoid serum-binding properties.
authors: Department of Food Science, Parco Gussone, 80055 Portici, Naples, Italy.
authors: Raffaele
authors: R
authors: Porta
authors: Concetta Valeria L
authors: CV
authors: Giosafatto
authors: Prospero
authors: P
authors: di Pierro
authors: Angela
authors: A
authors: Sorrentino
authors: Loredana
authors: L
authors: Mariniello
mesh_terms: D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry*; D021181:Egg Hypersensitivity / Q000276:immunology; D007106:Immune Sera / Q000737:chemistry; D010059:Ovomucin / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry; D011485:Protein Binding; D059748:Proteolysis; D013302:Streptomyces / Q000201:enzymology; D011503:Transglutaminases / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology
chemical_list: D000941:Antigens; D001426:Bacterial Proteins; D007106:Immune Sera; D010940:Plant Proteins; D014361:Trypsin Inhibitors; C048826:phaseolin protein, Phaseolus vulgaris; D010059:Ovomucin; D011503:Transglutaminases; D014357:Trypsin