title: Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: assessment of a molecular stepwise approach.
abstract: We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.
authors: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru. nicolas.veland@upch.pe
authors: Nicolas
authors: N
authors: Veland
authors: Andrea K
authors: AK
authors: Boggild
authors: Cristian
authors: C
authors: Valencia
authors: Braulio M
authors: BM
authors: Valencia
authors: Alejandro
authors: A
authors: Llanos-Cuentas
authors: Gert
authors: G
authors: Van der Auwera
authors: Jean-Claude
authors: JC
authors: Dujardin
authors: Jorge
authors: J
authors: Arevalo
mesh_terms: D000465:Algorithms; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D010568:Peru; D016133:Polymerase Chain Reaction / Q000379:methods*