title: Canine ASCT1 and ASCT2 are functional receptors for RD-114 virus in dogs.
abstract: All domestic cats carry an infectious endogenous retrovirus termed RD-114 virus. Recently, we and others found that several live-attenuated vaccines for dogs were contaminated with infectious RD-114 virus. In this study, we confirmed that the RD-114 virus efficiently infected and proliferated well in canine primary kidney cells, as well as three tested canine cell lines. Further, we identified canine ASCT1 and ASCT2, sodium-dependent neutral amino acid transporters, as RD-114 virus receptors. Canine ASCT2 also acts as a functional receptor for simian retrovirus 2, a pathogenic retrovirus that induces immunodeficiency in rhesus macaques. Identification of the canine receptor for RD-114 virus will help in evaluating the risk from vaccines contaminated by the virus.
authors: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Nihonmatsucho, Sakyo-ku, Kyoto 606-8501, Japan.|Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.|Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Rokusuke
authors: R
authors: Yoshikawa
authors: Department of Emerging Infectious Diseases, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
authors: Jiro
authors: J
authors: Yasuda
authors: Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Takeshi
authors: T
authors: Kobayashi
authors: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Nihonmatsucho, Sakyo-ku, Kyoto 606-8501, Japan.|Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Takayuki
authors: T
authors: Miyazawa
mesh_terms: D026962:Amino Acid Transport System ASC / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D020077:Endogenous Retroviruses / Q000502:physiology*; D016093:Mason-Pfizer monkey virus / Q000502:physiology; D011991:Receptors, Virus / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D026962:Amino Acid Transport System ASC; D011991:Receptors, Virus