title: Production of p-acetaminophenol by whole-cell catalysis using Escherichia coli overexpressing bacterial aryl acylamidase.
abstract: Aryl acylamidase (EC 3.5.1.13, AAA) acts on the amide bond between aryl and acyl groups. Whole cells of Escherichia coli overexpressing a novel bacterial AAA synthesized p-acetaminophenol (p-AAP) from p-aminophenol (p-AP, aryl compound) and acetate (acyl donor). Optimum conditions were pH 5.5 and 35°C with 100 mM p-AP and 600 mM sodium acetate in 100 mM sodium phosphate buffer including 1% (v/v) Triton X-100 for 60 h. 13.1 g p-AAP l(-1) was produced with a conversion yield of 87%.
authors: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-713, Korea.
authors: Hyeok-Jin
authors: HJ
authors: Ko
authors: Won-Gi
authors: WG
authors: Bang
authors: Kyoung Heon
authors: KH
authors: Kim
authors: In-Geol
authors: IG
authors: Choi
mesh_terms: D000082:Acetaminophen / Q000378:metabolism*; D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D000627:Aminophenols / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D019346:Sodium Acetate / Q000378:metabolism; D013696:Temperature
chemical_list: D000627:Aminophenols; D000082:Acetaminophen; D019346:Sodium Acetate; D000581:Amidohydrolases; C019732:aryl acylamidase; C026729:4-aminophenol