title: Oral delivery of double-stranded RNAs and siRNAs induces RNAi effects in the potato/tomato psyllid, Bactericerca cockerelli.
abstract: The potato/tomato psyllid, Bactericerca cockerelli (B. cockerelli), and the Asian citrus psyllid, Diaphorina citri (D. citri), are very important plant pests, but they are also vectors of phloem-limited bacteria that are associated with two devastating plant diseases. B. cockerelli is the vector of Candidatus Liberibacter psyllaurous (solanacearum), which is associated with zebra chip disease of potatoes, and D. citri is the vector of Ca. Liberibacter asiaticus, which is associated with the Huanglongbing (citrus greening) disease that currently threatens the entire Florida citrus industry. Here we used EST sequence information from D. citri to identify potential targets for RNA interference in B. cockerelli. We targeted ubiquitously expressed and gut-abundant mRNAs via injection and oral acquisition of double-stranded RNAs and siRNAs and were able to induce mortality in recipient psyllids. We also showed knockdown of target mRNAs, and that oral acquisition resulted primarily in mRNA knockdown in the psyllid gut. Concurrent with gene knockdown was the accumulation of target specific ∼ 21 nucleotide siRNAs for an abundant mRNA for BC-Actin. These results showed that RNAi can be a powerful tool for gene function studies in psyllids, and give support for continued efforts for investigating RNAi approaches as possible tools for psyllid and plant disease control.
authors: Department of Plant Pathology, University of California Davis, Davis, California, United States of America.
authors: Hada
authors: H
authors: Wuriyanghan
authors: Cristina
authors: C
authors: Rosa
authors: Bryce W
authors: BW
authors: Falk
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D001483:Base Sequence; D015536:Down-Regulation / Q000235:genetics; D006430:Hemiptera / Q000235:genetics*; D019476:Insect Proteins / Q000172:deficiency / Q000235:genetics; D018551:Solanum lycopersicum; D008845:Microinjections; D009928:Organ Specificity; D010944:Plants; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D011198:Solanum tuberosum; D059467:Transcriptome
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D034741:RNA, Small Interfering