title: Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe₃O₄ combined with cyclosporin A in murine models.
abstract: OBJECTIVE
  To investigate the effect of magnetic nanoparticles (MNPs) of Fe(3)O(4) combined with cyclosporin A (CsA) on acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in murine models.
  
  
  METHODS
  BALB/c mice preconditioned with total-body irradiation generated aGVHD and then were followed with allo-HSCT from allogeneic C57BL/6. Recipient mice were randomly divided into five groups and then given different supportive care and followed up. The physical signs and median survival time (MST) were recorded, peripheral blood cell counts were assessed, and histological changes of the main tissues were evaluated with hematoxylin-eosin staining. Furthermore, fluorescence polarization immunoassay was used to monitor the concentration of CsA.
  
  
  RESULTS
  The irradiated-only mice developed typical aGVHD, and the typical signs of aGVHD in the skin, liver, and intestine were observed by histopathological examination. Both CsA alone and in combination with Fe(3)O(4) MNPs significantly prolonged the MST of recipient mice compared with both the control and the Fe(3)O(4) MNPs groups. Notably, a combination of CsA with Fe(3)O(4) MNPs can elevate the peripheral white blood cells and alleviate the symptoms of GVHD and the pathological damage after allo-HSCT. In addition, the concentration of CsA was higher in plasma, heart, liver, and spleen of recipient mice with supporting care of the combination of CsA with Fe(3)O(4) MNPs than with CsA alone.
  
  
  CONCLUSIONS
  Taken together, Fe(3)O(4) MNPs may be used as a carrier of immunosuppressive agents to alleviate GVHD after allo-HSCT in murine models.
authors: Department of Hematology, Zhongda Hospital, Medical School, Southeast University, Nanjing, People's Republic of China.
authors: Jian
authors: J
authors: Cheng
authors: Ying
authors: Y
authors: Zhou
authors: Baoan
authors: B
authors: Chen
authors: Jun
authors: J
authors: Wang
authors: Guohua
authors: G
authors: Xia
authors: Nan
authors: N
authors: Jin
authors: Jiahua
authors: J
authors: Ding
authors: Chong
authors: C
authors: Gao
authors: Gouming
authors: G
authors: Chen
authors: Yushan
authors: Y
authors: Miao
authors: Weilan
authors: W
authors: Li
authors: Ziling
authors: Z
authors: Liu
authors: Xuemei
authors: X
authors: Wang
mesh_terms: D000818:Animals; D016572:Cyclosporine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006086:Graft vs Host Disease / Q000188:drug therapy / Q000517:prevention & control*; D018380:Hematopoietic Stem Cell Transplantation; D006651:Histocytochemistry; D007422:Intestines / Q000737:chemistry / Q000187:drug effects; D007958:Leukocyte Count; D007962:Leukocytes; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D012867:Skin / Q000737:chemistry / Q000187:drug effects; D016019:Survival Analysis; D014184:Transplantation, Homologous
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D016572:Cyclosporine