title: Semi-quantitative analysis of influenza samples using the Luminex xTAG(®) respiratory viral panel kit.
abstract: The Luminex xTAG(®) respiratory viral panel (RVP) kit simultaneously detects and identifies multiple respiratory viruses including several subtypes of influenza A using a multiplex nucleic acid amplification test assay platform. The emitted fluorescence signal from the RVP assay provides qualitative information on the presence of a particular viral species in respiratory specimens. However, a quantitative assessment is preferred when monitoring environmental samples for respiratory viruses. In this study, we explored the potential use of the RVP kit as a semi-quantitative screening assay for influenza virus detection. The concentration- response of the RVP assay was modeled using four-parameter logistic (4-PL) fits of mean fluorescence intensity (MFI) versus dilute ranges of the influenza A matrix gene, seasonal influenza vaccine, and 2009 H1N1 influenza vaccine. The goodness of fit of the 4-PL model was evaluated by comparing the copy number determined with the fitted model (observed copy number) with the copy number calculated from the dilution of the matrix DNA or vaccine (expected copy number). For the matrix DNA and 2009 H1N1 vaccine, the 4-PL model provided good fit for the influenza A RVP assay response over factors of 10(3) to 10(4). For seasonal influenza vaccine, the model provided good fit for RVP assay response to influenza A, influenza B, H1, and H3.
authors: Biomonitoring and Health Assessment Branch, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, Cincinnati, Ohio, USA. jps3@cdc.gov
authors: Jerome
authors: J
authors: Smith
authors: Deborah
authors: D
authors: Sammons
authors: Christine
authors: C
authors: Toennis
authors: Mary Ann
authors: MA
authors: Butler
authors: Francoise
authors: F
authors: Blachere
authors: Donald
authors: D
authors: Beezhold
mesh_terms: D005453:Fluorescence; D018628:Gene Dosage; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D009981:Influenza B virus / Q000235:genetics; D007252:Influenza Vaccines / Q000627:therapeutic use; D007251:Influenza, Human / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D016014:Linear Models; D016015:Logistic Models; D060885:Multiplex Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D007252:Influenza Vaccines; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic