title: Evaluation of the magnetic field requirements for nanomagnetic gene transfection.
abstract: The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency.
  
  
  METHODS
  non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays.
  
  
  RESULTS
  Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically.
  
  
  CONCLUSIONS
  In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™.
authors: Institute for Science & Technology in Medicine, Hartshill, Stoke-on-Trent, UK.
authors: A
authors: A
authors: Fouriki
authors: N
authors: N
authors: Farrow
authors: M A
authors: MA
authors: Clements
authors: J
authors: J
authors: Dobson