title: Monitoring of urinary messenger RNA levels for the prediction of flare in systemic lupus erythematosus.
abstract: BACKGROUND
  Systemic lupus erythematosus (SLE) is characterized by disease flares and remission. We hypothesize that in clinically quiescent SLE patients, the mRNA level of target genes in the urinary sediment is an early indicator of disease flare.
  
  
  METHODS
  From a cohort of 134 adult SLE patients prospectively followed for 56 weeks, we identified 19 patients with a single disease flare. The mRNA level of eight pre-defined target genes in their urinary sediment before disease flare was compared to 19 matched controls with no disease flare during the same period.
  
  
  RESULTS
  Urinary mRNA level remained static in the control group during the study period. Before disease flare, there was a significant increase in the mRNA level of monocyte chemotactic protein (MCP)-1 and forkhead box P3 (FOXP3), and decrease in interleukin (IL)-17 and GATA-3, in the urinary sediment. The mRNA level of FOXP3 in urinary sediment increases 8 weeks prior to a flare, which precedes the corresponding change in serum complement and anti-DNA antibody titer, while that of MCP-1, IL-17, and GATA3 began to change 4 weeks prior to a flare. The same pattern of change in urinary mRNA level was observed in patients with mild-to-moderate or severe flare, and those with renal or non-renal flare. The SLE Disease Activity Index (SLEDAI) score at the time of flare significantly correlated with the change in urinary level of IL-17 (r=-0.462, p=0.046) and GATA-3 (r=-0.455, p=0.05), but not MCP-1 or FOXP3, prior to the flare.
  
  
  CONCLUSIONS
  Monitoring of MCP-1, IL-17, GATA-3 and FOXP3 mRNA level in urinary sediment may provide an early clue for detecting disease flare in SLE patients.
authors: Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China. ccszeto@cuhk.edu.hk
authors: Cheuk-Chun
authors: CC
authors: Szeto
authors: Lai-Shan
authors: LS
authors: Tam
authors: Bonnie Ching-Ha
authors: BC
authors: Kwan
authors: Ka-Bik
authors: KB
authors: Lai
authors: Gang
authors: G
authors: Wang
authors: Edmund Kwok-Ming
authors: EK
authors: Li
authors: Kai-Ming
authors: KM
authors: Chow
authors: Fernand Mac-Moune
authors: FM
authors: Lai
authors: Philip Kam-Tao
authors: PK
authors: Li
mesh_terms: D000328:Adult; D016207:Cytokines / Q000652:urine; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000235:genetics / Q000652:urine*; D008297:Male; D012333:RNA, Messenger / Q000652:urine; D014157:Transcription Factors / Q000652:urine
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D014157:Transcription Factors