title: Characterization of invading glioma cells using molecular analysis of leading-edge tissue.
abstract: OBJECTIVE
  We have introduced a method of characterization of invading glioma cells by using molecular analysis of marginal invading tumor cells and molecular profiles of glioma tumor margin.
  
  
  METHODS
  Each of tumor core and marginal tissues was obtained in 22 glioma patients. Tumor core cells and marginal cells from each glial tumor were collected by laser capture microdissection or intraoperative microdissection under the operating microscope. Expression of MMP-2, MMP-9, CD44 and RHAMM mRNA by invading glioma cells compared with tumor core was confirmed by realtime-PCR of twenty-four glioma specimens. Clinical data also were reviewed for invasion and recurrence pattern of the gliomas radiologically and invasive rim pattern microscopically.
  
  
  RESULTS
  Overall results of the molecular analysis showed that relative overexpression of MMP-2, MMP-9 and RHAMM were noted at the invasive edge of human glioma specimens comparing to the tumor core but CD44 was highly expressed in the tumor core comparing to the margin. High marginal expression of MMP-2 and MMP-9 were noted in poorly ill-defined margin on the pathological finding. High marginal expression of CD44 and MMP-2 were demonstrated in the midline cross group on the radiological review, and that of RHAMM and MMP-2 were showed in the aggressive recurrence group. High expression of MMP-2 seems to be involved in the various invasion-related phenomenons.
  
  
  CONCLUSIONS
  Up-regulation of MMP-2, MMP-9, CD44 and RHAMM was noted in invasive edge of gliomas according to the various clinical situations.
authors: Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, the Brain Korea 21 Project, Chonnam National University Hwasun Hospital & Medical School, Gwangju, Korea.
authors: Cheol-Soo
authors: CS
authors: Kim
authors: Shin
authors: S
authors: Jung
authors: Tae-Young
authors: TY
authors: Jung
authors: Woo-Youl
authors: WY
authors: Jang
authors: Heung-Suk
authors: HS
authors: Sun
authors: Hyang-Hwa
authors: HH
authors: Ryu