title: Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells.
abstract: B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.
authors: Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA.
authors: Youngjo
authors: Y
authors: Kim
authors: Alexei A
authors: AA
authors: Sharov
authors: Katie
authors: K
authors: McDole
authors: Melody
authors: M
authors: Cheng
authors: Haiping
authors: H
authors: Hao
authors: Chen-Ming
authors: CM
authors: Fan
authors: Nicholas
authors: N
authors: Gaiano
authors: Minoru S H
authors: MS
authors: Ko
authors: Yixian
authors: Y
authors: Zheng
mesh_terms: D000818:Animals; D049628:Body Size; D001921:Brain / Q000166:cytology / Q000196:embryology; D002453:Cell Cycle; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D047108:Embryonic Development; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D058953:Neural Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology; D034881:Nuclear Lamina / Q000502:physiology; D009929:Organ Size; D038081:Organogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology; D011401:Promoter Regions, Genetic; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000166:cytology
chemical_list: D002843:Chromatin; D034921:Lamin Type B