title: Mucoadhesive alginate/poly (L-lysine)/thiolated alginate microcapsules for oral delivery of Lactobacillus salivarius 29.
abstract: In this study, thiolated alginate was synthesized by introduction of cysteine to alginate to prepare mucoadhesive alginate/poly (L-lysine)/thiolated alginate (APTA) microcapsules for efficient oral delivery of Lactobacillus salivarius 29 (LS29), a novel therapeutic Lactobacillus strain, in vitro and in vivo. About 759 +/- 32.4 microM of cysteine per gram of alginate was introduced by estimation of Ellman's reagent reaction. LS29-loaded APTA microcapsules provided suitable morphology, size, and a high loading content and efficiency. LS29 in LS29-loaded APTA microcapsules were effectively protected from simulated gastric condition (pH 2.0) than that of unprotected LS29. LS29 were released from APTA microcapsules in simulated intestinal condition (pH 7.2) with a time-dependent manner. The in vitro and in vivo mucoadhesion study suggested that APTA microcapsules had remarkably stronger mucoadhesive property and provided a promising delivery system for oral administration of LS29.
authors: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, South Korea.
authors: Mohammad Ariful
authors: MA
authors: Islam
authors: Prati
authors: P
authors: Bajracharya
authors: Sang-Kee
authors: SK
authors: Kang
authors: Cheol-Heui
authors: CH
authors: Yun
authors: Eun-Mi
authors: EM
authors: Kim
authors: Hwan-Jeong
authors: HJ
authors: Jeong
authors: Yun-Jaie
authors: YJ
authors: Choi
authors: Eun-Bae
authors: EB
authors: Kim
authors: Chong-Su
authors: CS
authors: Cho
mesh_terms: D000269:Adhesives; D000284:Administration, Oral; D000464:Alginates / Q000737:chemistry*; D002214:Capsules; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa; D007778:Lactobacillus; D011107:Polylysine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000269:Adhesives; D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D013438:Sulfhydryl Compounds; D011107:Polylysine; D020723:Glucuronic Acid