title: Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate.
abstract: Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
authors: Department of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors: Min-Kyoung
authors: MK
authors: Shin
authors: Myung Hwan
authors: MH
authors: Jung
authors: Won-Jung
authors: WJ
authors: Lee
authors: Pil Son
authors: PS
authors: Choi
authors: Yong-Suk
authors: YS
authors: Jang
authors: Han Sang
authors: HS
authors: Yoo
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000517:prevention & control*; D016977:Actinobacillus pleuropneumoniae; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002772:Cholera Toxin / Q000737:chemistry*; D005260:Female; D006460:Hemolysin Proteins / Q000276:immunology*; D007117:Immunization, Secondary; D051379:Mice; D008813:Mice, Inbred ICR; D030821:Plants, Genetically Modified; D003313:Zea mays / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; C085361:ApxII toxin, bacteria; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D002772:Cholera Toxin