title: Enhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis.
abstract: Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORγt, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORγt, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.
authors: Department of Immunology, Institute of Laboratory Medicine, Jiangsu University, Xuefu Road 301, Zhenjiang 212013, China.
authors: Yan
authors: Y
authors: Shi
authors: Siamak
authors: S
authors: Sandoghchian Shotorbani
authors: Zhaoliang
authors: Z
authors: Su
authors: Yanfang
authors: Y
authors: Liu
authors: Jia
authors: J
authors: Tong
authors: Dong
authors: D
authors: Zheng
authors: Jianguo
authors: J
authors: Chen
authors: Yingzhao
authors: Y
authors: Liu
authors: Yan
authors: Y
authors: Xu
authors: Zhijun
authors: Z
authors: Jiao
authors: Shengjun
authors: S
authors: Wang
authors: Liwei
authors: L
authors: Lu
authors: Xinxiang
authors: X
authors: Huang
authors: Huaxi
authors: H
authors: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005260:Female; D024243:HMGB1 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D024243:HMGB1 Protein; D012333:RNA, Messenger