title: Absence of ocular malignant transformation after sub-retinal delivery of rAAV2/2 or integrating lentiviral vectors in p53-deficient mice.
abstract: Insertional mutagenesis following gene therapy with gammaretroviral vectors can cause the development of lymphoproliferation in children with X-linked severe combined immunodeficiency. In experimental studies, recombinant adeno-associated virus (rAAV) vectors have also been reported to increase susceptibility to carcinogenesis. The possibility of vector-induced transformation in quiescent ocular cells is probably significantly lower than in mitotically active cells, but given the increasing number of clinical applications of rAAV and lentiviral vectors for ocular disease, a specific assessment of their oncogenic potential in the eye is important. In this study, we investigated the effect of rAAV2/2 and integrating HIV-1 vectors upon the incidence of ocular neoplasia in p53 tumour-suppressor gene-knockout (p53(-/-)) mice, which are highly susceptible to intraocular malignant transformation. Subretinal injections of high titre rAAV2/2 or integrating HIV-1 vectors induced no tumours in p53(-/-) or p53(+/-) animals, nor significantly affected their natural longevity. We conclude that any insertional events arising from subretinal delivery of these vectors appear insufficient to cause intraocular malignancy, even in highly susceptible animals. These findings support the continued development of these vectors for ocular applications.
authors: Department of Genetics, Institute of Ophthalmology, London, UK. kambalaggan@yahoo.co.uk
authors: K S
authors: KS
authors: Balaggan
authors: Y
authors: Y
authors: Duran
authors: A
authors: A
authors: Georgiadis
authors: C
authors: C
authors: Thaung
authors: S E
authors: SE
authors: Barker
authors: P K
authors: PK
authors: Buch
authors: A
authors: A
authors: MacNeil
authors: S
authors: S
authors: Robbie
authors: J W B
authors: JW
authors: Bainbridge
authors: A J
authors: AJ
authors: Smith
authors: R R
authors: RR
authors: Ali
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D004596:Electroretinography; D005134:Eye Neoplasms / Q000235:genetics; D055786:Gene Knockout Techniques; D018014:Gene Transfer Techniques / Q000009:adverse effects*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000009:adverse effects*; D049452:Green Fluorescent Proteins; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D012160:Retina; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53; D049452:Green Fluorescent Proteins