title: Differential gene expression in migratory streams of cortical interneurons.
abstract: Cortical interneurons originate in the ganglionic eminences of the subpallium and migrate into the cortex in well-defined tangential streams. At the start of corticogenesis, two streams of migrating neurons are evident: a superficial one at the level of the preplate (PPL), and a deeper one at the level of the intermediate zone (IZ). Currently, little is known about the signalling mechanisms that regulate interneuron migration, and almost nothing is known about the molecules that may be involved in their choice of migratory stream. Here, we performed a microarray analysis, comparing the changes in gene expression between cells migrating in the PPL and those migrating in the IZ at embryonic day 13.5. This analysis identified genes, many of them novel, that were upregulated in one of the two streams. Moreover, polymerase chain reaction, in situ hybridization experiments and immunohistochemistry showed the expression of these genes in interneurons migrating within the PPL or IZ, suggesting that they play a role in their migration and choice of stream.
authors: Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors: Mary
authors: M
authors: Antypa
authors: Clare
authors: C
authors: Faux
authors: Gregor
authors: G
authors: Eichele
authors: John G
authors: JG
authors: Parnavelas
authors: William D
authors: WD
authors: Andrews
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000502:physiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D046228:Microarray Analysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1