title: Conditioned taste aversion dependent regulation of amygdala gene expression.
abstract: The present experiments investigated gene expression in the amygdala following contingent taste/LiCl treatment that supports development of conditioned taste aversion (CTA). The use of whole genome chips and stringent data set filtering led to the identification of 168 genes regulated by CTA compared to non-contingent LiCl treatment that does not support CTA learning. Seventy-six of these genes were eligible for network analysis. Such analysis identified "behavior" as the top biological function, which was represented by 15 of the 76 genes. These genes included several neuropeptides, G protein-coupled receptors, ion channels, kinases, and phosphatases. Subsequent qRT-PCR analyses confirmed changes in mRNA expression for 5 of 7 selected genes. We were able to demonstrate directionally consistent changes in protein level for 3 of these genes; insulin 1, oxytocin, and major histocompatibility complex class I-C. Behavioral analyses demonstrated that blockade of central insulin receptors produced a weaker CTA that was less resistant to extinction. Together, these results support the notion that we have identified downstream genes in the amygdala that contribute to CTA learning.
authors: Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, KY, United States.
authors: Siva K
authors: SK
authors: Panguluri
authors: Nobuyuki
authors: N
authors: Kuwabara
authors: Yi
authors: Y
authors: Kang
authors: Nigel
authors: N
authors: Cooper
authors: Robert F
authors: RF
authors: Lundy
mesh_terms: D000679:Amygdala / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology*; D005108:Extinction, Psychological / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D007328:Insulin / Q000235:genetics / Q000502:physiology*; D018021:Lithium Chloride / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin / Q000037:antagonists & inhibitors / Q000502:physiology*; D013649:Taste / Q000502:physiology*
chemical_list: D007328:Insulin; D010455:Peptides; C532752:S961 peptide; D011972:Receptor, Insulin; D018021:Lithium Chloride