title: Enhanced anthocyanins and resveratrol production in Vitis vinifera cell suspension culture by indanoyl-isoleucine, N-linolenoyl-L-glutamine and insect saliva.
abstract: The effects of two synthetic elicitor indanoyl-isoleucine (In-Ile), N-linolenoyl-L-glutamine (Lin-Gln) and one biotic elicitor insect saliva (from Manduca sexta larvae) on plant cell cultures with respect to the induction of secondary metabolite production were investigated. Stimulated production of secondary metabolites, particularly anthocyanins in plant cells and phenolic acids in culture medium, was studied by using suspension culture of Vitis vinifera L. cv. Gamay Fréaux as a model system. In the treatments with In-Ile, the production of anthocyanins was enhanced 2.6-fold. In-Ile, Lin-Gln and saliva significantly elevated the accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol. The used elicitors did not suppress cell growth. Secondary metabolites were differently responsive to elicitation. 3-O-glucosyl-resveratrol was the predominant phenolic acid in V. vinifera cell culture, and its production was significantly stimulated by saliva, with 7.0-fold of the control level 24 h after treatment. The production of 4-(3,5-dihydroxy-phenyl)-phenol was significantly stimulated by In-Ile with 6.4-fold of the control level 24 h after treatment.
authors: Institute of Food Biotechnology and Food Chemistry, Berlin University of Technology, Königin-Luise Str. 22, 14195 Berlin, Germany. zhenzhen.cai@mailbox.tu-berlin.de
authors: Zhenzhen
authors: Z
authors: Cai
authors: Dietrich
authors: D
authors: Knorr
authors: Iryna
authors: I
authors: Smetanska
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000096:biosynthesis*; D018929:Cell Culture Techniques; D003470:Culture Media; D005524:Food Technology; D005973:Glutamine / Q000031:analogs & derivatives / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007189:Indans / Q000494:pharmacology; D007532:Isoleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D018402:Manduca / Q000737:chemistry; D000077185:Resveratrol; D012463:Saliva / Q000737:chemistry; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D003470:Culture Media; D007189:Indans; D008042:Linolenic Acids; C475661:N-linolenoyl-L-glutamine; D013267:Stilbenes; D007532:Isoleucine; D005973:Glutamine; D000077185:Resveratrol