title: Effects of Angelica acutiloba on mast cell-mediated allergic reactions in vitro and in vivo.
abstract: The root of Angelica acutiloba is a widely used herbal medicine which has been used as a typical therapeutic for allergic diseases in traditional medicine. This study was aimed to investigate the effects of A. acutiloba on allergic reactions in in vitro and in vivo models and its mechanism of action. A. acutiloba was extracted by maceration with 80% ethanol (AAE) and standardized by high-performance liquid chromatography. We investigated the effect of AAE on phorbol-12-myristate-13-acetate plus calcium ionophore A23187 (PMACI)-induced cytokine release; phosphorylation of JNK, ERK, and p38 in human mast cell-1 (HMC-1); and compound 48/80-induced release of histamine in rat peritoneal mast cells (RPMCs). We also investigated the effects on Evans blue (EB) extravasation induced by anti-DNP IgE in rats. Treatment with 1, 10 and 100 μg/ml AAE concentration-dependently inhibited the release of cytokines (tumor necrosis factor-α, interleukin (IL) -6, and IL-8) and phosphorylation of ERK and JNK induced by PMACI in HMC-1 cells, but it did not inhibit the phosphorylation of p38. It also inhibited compound 48/80-induced histamine release in RPMCs. Oral administration of 271 mg/kg AAE inhibited EB extravasation in a passive cutaneous anaphylaxis rat model. In conclusion, AAE inhibited mast cell-derived allergic reactions by inhibiting the release of histamine, the production of pro-inflammatory cytokines, and the phosphorylation of ERK and JNK.
authors: Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors: Kyungjin
authors: K
authors: Lee
authors: Youngjoo
authors: Y
authors: Sohn
authors: Min-Jung
authors: MJ
authors: Lee
authors: Hyun-Sam
authors: HS
authors: Cho
authors: Min-Hee
authors: MH
authors: Jang
authors: Na-Young
authors: NY
authors: Han
authors: Kyu-Won
authors: KW
authors: Shin
authors: Sung-Hoon
authors: SH
authors: Kim
authors: Ik-Hyun
authors: IH
authors: Cho
authors: Youngmin
authors: Y
authors: Bu
authors: Hyuk-Sang
authors: HS
authors: Jung
mesh_terms: D029969:Angelica; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000473:pathology; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D061207:Calcium Ionophores; D002273:Carcinogens; D045424:Complex Mixtures; D016207:Cytokines; D000001:Calcimycin; D048049:Extracellular Signal-Regulated MAP Kinases; D048670:MAP Kinase Kinase 4; D013755:Tetradecanoylphorbol Acetate