title: Effect of O-glycosylation and tyrosine sulfation of leech-derived peptides on binding and inhibitory activity against thrombin.
abstract: Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech Hirudinaria manillensis) is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.
authors: School of Chemistry, The University of Sydney, NSW 2006, Australia.
authors: Yves S Y
authors: YS
authors: Hsieh
authors: Deni
authors: D
authors: Taleski
authors: Brendan L
authors: BL
authors: Wilkinson
authors: Lakshmi C
authors: LC
authors: Wijeyewickrema
authors: Ty E
authors: TE
authors: Adams
authors: Robert N
authors: RN
authors: Pike
authors: Richard J
authors: RJ
authors: Payne
mesh_terms: D001665:Binding Sites / Q000187:drug effects; D006031:Glycosylation; D008968:Molecular Conformation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013917:Thrombin / Q000037:antagonists & inhibitors*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D010455:Peptides; D014443:Tyrosine; D013917:Thrombin