title: Hemolytic effect of polymeric nanoparticles: role of albumin.
abstract: Nanoparticles (NP) have drawn increasing interest from many fields in medicine and are a relatively new class of biomedical products. Because there are concerns about the health effects of nanoparticles, it is important to understand how nanoparticles interact specifically with red blood cells (RBC), a central object in the blood circulation. As numerous studies that have examined NP/RBC interaction concentrated on the hemolytic potential of nanoparticles, we describe an investigation of hemolytic activity of polystyrene nanoparticles (PS-NP) in protein free medium and its modulation by albumin. We found that treatment of RBCs with PS-NP induces hemolysis (dose and particle size dependent) in plasma free medium but not in full plasma or in buffer, which contain albumin. Critical albumin concentration is 0.05% wt. According to our results hemolytic effect of nanoparticles is strongly modulated by protein concentration in the medium.
authors: Department of Biochemistry, Hebrew University, Jerusalem, Israel. gregb@cc.huji.ac.il
authors: G
authors: G
authors: Barshtein
authors: D
authors: D
authors: Arbell
authors: S
authors: S
authors: Yedgar
mesh_terms: D000418:Albumins / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D053758:Nanoparticles; D010316:Particle Size; D011108:Polymers / Q000494:pharmacology*
chemical_list: D000418:Albumins; D011108:Polymers