title: Whole blood leukocytes isolation with microfabricated filter for cell analysis.
abstract: The flow cytometric analysis of leukocytes in whole blood usually requires isolation of leukocytes from other components of whole blood. Density gradient centrifugation and red blood cell lysis are the most commonly used methods to separate leukocytes but come with significant limitations. We report the results of the evaluation of a microfabricated filtration device for blood preparation that separates erythrocytes from leukocytes based on their size and mechanical properties. The microfabricated filter evaluated here requires a rapid and simple procedure and results in high leukocytes recovery without introducing bias among the leukocyte subpopulations. The filter removes erythrocytes, platelets, plasma proteins, and unbound staining reagent. This gentle filtration process produces very clean stained leukocytes for cytometric analysis without any apparent damage to leukocytes.
authors: BD Biosciences, San Jose, California 95131, USA. Liping_Yu@bd.com
authors: Liping
authors: L
authors: Yu
authors: Patrick
authors: P
authors: Warner
authors: Brian
authors: B
authors: Warner
authors: Diether
authors: D
authors: Recktenwald
authors: Douglas
authors: D
authors: Yamanishi
authors: Antonio
authors: A
authors: Guia
authors: Andrea
authors: A
authors: Ghetti
mesh_terms: D017209:Apoptosis / Q000502:physiology; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival / Q000502:physiology; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D046210:Microfluidic Analytical Techniques; D008851:Micropore Filters