title: Role of intracellular proteases in the antibiotic resistance, motility, and biofilm formation of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa possesses complex regulatory networks controlling virulence and survival under adverse conditions, including antibiotic pressure, which are interconnected and share common regulatory proteins. Here, we screen a panel of 13 mutants defective in intracellular proteases and demonstrate that, in addition to the known alterations in Lon and AsrA mutants, mutation of three protease-related proteins PfpI, ClpS, and ClpP differentially affected antibiotic resistance, swarming motility, and biofilm formation.
authors: Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada.
authors: Lucía
authors: L
authors: Fernández
authors: Elena B M
authors: EB
authors: Breidenstein
authors: Diana
authors: D
authors: Song
authors: Robert E W
authors: RE
authors: Hancock
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009068:Movement / Q000187:drug effects*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010450:Endopeptidases