title: Rotor architecture in the yeast and bovine F1-c-ring complexes of F-ATP synthase.
abstract: The F(1)F(O)-ATP synthase is a rotary molecular nanomotor. F(1) is a chemical motor driven by ATP hydrolysis while F(O) is an electrical motor driven by the proton flow. The two stepping motors are mechanically coupled through a common rotary shaft. Up to now, the three available crystal structures of the F(1)c(10) sub-complex of the yeast F(1)F(O)-ATP synthase were isomorphous and then named yF(1)c(10)(I). In this crystal form, significant interactions of the c(10)-ring with the F(1)-head of neighboring molecules affected the overall conformation of the F(1)-c-ring complex. The symmetry axis of the F(1)-head and the inertia axis of the c-ring were tilted near the interface between the F(1)-central stalk and the c-ring rotor, resulting in an unbalanced machine. We have solved a new crystal form of the F(1)c(10) complex, named yF(1)c(10)(II), inhibited by adenylyl-imidodiphosphate (AMP-PNP) and dicyclohexylcarbodiimide (DCCD), at 6.5Å resolution in which the crystal packing has a weaker influence over the conformation of the F(1)-c-ring complex. yF(1)c(10)(II) provides a model of a more efficient generator. yF(1)c(10)(II) and bovine bF(1)c(8) structures share a common rotor architecture with the inertia center of the F(1)-stator close to the rotor axis.
authors: Univ. de Bordeaux, IBGC, UMR 5095, F-33000 Bordeaux, France. marie-france.giraud@ibgc.cnrs.fr
authors: Marie-France
authors: MF
authors: Giraud
authors: Patrick
authors: P
authors: Paumard
authors: Corinne
authors: C
authors: Sanchez
authors: Daniel
authors: D
authors: Brèthes
authors: Jean
authors: J
authors: Velours
authors: Alain
authors: A
authors: Dautant
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D040681:Structural Homology, Protein; D013499:Surface Properties
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D006180:Proton-Translocating ATPases