title: The combination of organoselenium compounds and guanosine prevents glutamate-induced oxidative stress in different regions of rat brains.
abstract: This study was designed to investigate the protective effects of the combination of guanosine and 2 organoselenium compounds (ebselen and diphenyl diselenide) against glutamate-induced oxidative stress in different regions of rat brains. Glutamate caused an increase in reactive oxygen species (ROS) generation and a decrease in [(3)H]-glutamate uptake in striatal, cortical, and hippocampal slices. Guanosine, ebselen, and diphenyl diselenide prevented glutamate-induced ROS production in striatal, cortical and hippocampal slices. The combination of guanosine with organoselenium compounds was more effective against glutamate-induced ROS production than the individual compounds alone. Guanosine prevented [(3)H]-glutamate uptake inhibition in striatal, cortical, and hippocampal slices. Thus, protection against the harmful effects of glutamate is possibly due to the combination of the antioxidant properties of organoselenium compounds and the stimulatory effect of guanosine on glutamate uptake. In conclusion, the combination of antioxidants and glutamatergic system modulators could be considered a potential therapy against the prooxidant effects of glutamate.
authors: Departamento de Química, Programa de Pós-graduação em Ciências Biológicas, Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas,Universidade Federal de Santa Maria, Cep 97105-900, Santa Maria, RS, Brazil.
authors: Cristiane L
authors: CL
authors: Dalla Corte
authors: Luíza L
authors: LL
authors: Bastos
authors: Fernando
authors: F
authors: Dobrachinski
authors: João B T
authors: JB
authors: Rocha
authors: Félix A A
authors: FA
authors: Soares
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001393:Azoles / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D001928:Brain Diseases, Metabolic / Q000188:drug therapy* / Q000503:physiopathology; D004195:Disease Models, Animal; D004357:Drug Synergism; D004359:Drug Therapy, Combination / Q000379:methods; D018698:Glutamic Acid / Q000378:metabolism / Q000502:physiology / Q000633:toxicity*; D006151:Guanosine / Q000494:pharmacology*; D054833:Isoindoles; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity; D009924:Organ Culture Techniques; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001393:Azoles; D001555:Benzene Derivatives; D054833:Isoindoles; D018696:Neuroprotective Agents; D009498:Neurotoxins; D016566:Organoselenium Compounds; D006151:Guanosine; C061132:diphenyldiselenide; D018698:Glutamic Acid; C042986:ebselen