title: Novel genetic tools for Hansenula polymorpha.
abstract: Hansenula polymorpha is an important yeast in industrial biotechnology. In addition, it is extensively used in fundamental research devoted to unravel the principles of peroxisome biology and nitrate assimilation. Here we present an overview of key components of the genetic toolbox for H. polymorpha. In addition, we present new selection markers that we recently implemented in H. polymorpha. We describe novel strategies for the efficient creation of targeted gene deletions and integrations in H. polymorpha. For this, we generated a yku80 mutant, deficient in non-homologous end joining, resulting in strongly enhanced efficiency of gene targeting relative to the parental strain. Finally, we show the implementation of Gateway technology and a single-step PCR strategy to create deletions in H. polymorpha.
authors: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, Groningen, The Netherlands.
authors: Ruchi
authors: R
authors: Saraya
authors: Arjen M
authors: AM
authors: Krikken
authors: Jan A K W
authors: JA
authors: Kiel
authors: Richard J S
authors: RJ
authors: Baerends
authors: Marten
authors: M
authors: Veenhuis
authors: Ida J
authors: IJ
authors: van der Klei
mesh_terms: D001709:Biotechnology / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
chemical_list: D005656:Fungal Proteins