title: A Portable Analyzer for Pouch-Actuated, Immunoassay Cassettes.
abstract: A portable, small footprint, light, general purpose analyzer (processor) to control the flow in immunoassay cassettes and to facilitate the detection of test results is described. The durable analyzer accepts disposable cassettes that contain pouches and reaction chambers for various unit operations such as hydration of dry reagents, stirring, and incubation. The analyzer includes individually controlled, linear actuators to compress the pouches in the cassette, which facilitates the pumping and mixing of sample and reagents, and to close diaphragm-based valves for flow control. The same types of actuators are used to compress pouches and actuate valves. The analyzer also houses a compact OEM scanner/reader to excite fluorescence and detect emission from labels. The analyzer is hydraulically isolated from the cassette, reducing the possibility of cross-contamination. The analyzer facilitates programmable, automated execution of a sequence of operations such as pumping and valving in a timely fashion, reducing the level of expertise required from the operator and the possibility for errors. The analyzer's design is modular and expandable to accommodate cassettes of various complexities and additional functionalities. In this paper, the utility of the analyzer has been demonstrated with the execution of a simple, consecutive, lateral flow assay of a model biological system and the test results were detected with up converting phosphor labels that are excited at infrared frequencies and emit in the visible spectrum.
authors: Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, 220 S. 33rd St., Philadelphia, PA 19104.
authors: Xianbo
authors: X
authors: Qiu
authors: Changchun
authors: C
authors: Liu
authors: Michael G
authors: MG
authors: Mauk
authors: Robert W
authors: RW
authors: Hart
authors: Dafeng
authors: D
authors: Chen
authors: Jing
authors: J
authors: Qiu
authors: Terry
authors: T
authors: Kientz
authors: Jonathan
authors: J
authors: Fiene
authors: Haim H
authors: HH
authors: Bau