title: Highly potent fibrinolytic serine protease from Streptomyces.
abstract: We introduce a highly potent fibrinolytic serine protease from Streptomyces omiyaensis (SOT), which belongs to the trypsin family. The fibrinolytic activity of SOT was examined using in vitro assays and was compared with those of known fibrinolytic enzymes such as plasmin, tissue-type plasminogen activator (t-PA), urokinase, and nattokinase. Compared to other enzymes, SOT showed remarkably higher hydrolytic activity toward mimic peptides of fibrin and plasminogen. The fibrinolytic activity of SOT is about 18-fold higher than that of plasmin, and is comparable to that of t-PA by fibrin plate assays. Furthermore, SOT had some plasminogen activator-like activity. Results show that SOT and nattokinase have very different fibrinolytic and fibrinogenolytic modes, engendering significant synergetic effects of SOT and nattokinase on fibrinolysis. These results suggest that SOT presents important possibilities for application in the therapy of thrombosis.
authors: Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan.
authors: Yoshiko
authors: Y
authors: Uesugi
authors: Hirokazu
authors: H
authors: Usuki
authors: Masaki
authors: M
authors: Iwabuchi
authors: Tadashi
authors: T
authors: Hatanaka
mesh_terms: D005337:Fibrin / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000378:metabolism* / Q000627:therapeutic use; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D057057:Serine Proteases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013381:Subtilisins / Q000378:metabolism; D015912:Thrombolytic Therapy / Q000379:methods*; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
chemical_list: D005343:Fibrinolytic Agents; D005337:Fibrin; D057057:Serine Proteases; D013381:Subtilisins; D010959:Tissue Plasminogen Activator; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator; C053771:nattokinase