title: Allyl isothiocyanate as a potential inducer of paraoxonase-1--studies in cultured hepatocytes and in mice.
abstract: In this study, we tested the ability of structure-related isothiocyanates to induce the antiatherogenic enzyme paraoxonase-1 (PON1) in cultured hepatocytes. Allyl isothiocyanate (AITC), phenylethyl isothiocyanate (PEITC), and sulforaphane (SFN), but not butyl isothiocyanate (BITC) resulted in dose-dependent induction of PON1 transactivation in Huh7 cells in vitro. Induction of PON1 due to AITC was inhibited by the selective peroxisome proliferator-activated receptor γ-antagonist T0070907. AITC was used in a subsequent in vivo study in mice (n = 10 per group, Western-type diet) to test its PON1 inducing activity. Unlike in cultured hepatocytes, AITC supplementation (15 mg/kg body weight) did not increase hepatic PON1 mRNA and protein levels in mice. Thus, it is suggested that AITC may be a potent inducer of PON1 in vitro, but not in mouse liver in vivo.
authors: Devision of Food Science, Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Kiel, Germany. cschrader@foodsci.uni-kiel.de
authors: Charlotte
authors: C
authors: Schrader
authors: Anne-Christin
authors: AC
authors: Graeser
authors: Patricia
authors: P
authors: Huebbe
authors: Anika E
authors: AE
authors: Wagner
authors: Gerald
authors: G
authors: Rimbach
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects*; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C058305:phenethyl isothiocyanate; C004471:allyl isothiocyanate; D043303:Aryldialkylphosphatase; C016766:sulforaphane