title: Establishment of a highly migratory subclone reveals that CD133 contributes to migration and invasion through epithelial-mesenchymal transition in pancreatic cancer.
abstract: Pancreatic cancer is a lethal disease because of invasion and early metastasis. Although CD133, a marker of cancer stem cells (CSCs) in a variety of solid tumors, has been studied in recent decades, its function remains obscure. Recent reports suggest that epithelial-mesenchymal transition (EMT) may be related to the properties of CSCs. In this study, we investigated whether CSC markers are associated with EMT. For Capan1M9, a highly migratory cell subclone established from human pancreatic cancer cell line Capan-1, CD133 expression, migration, and invasion were greater than for the parent cells. In Capan1M9 cells, the EMT-related transcription factors Slug and Snail were up-regulated, and N-cadherin and fibronectin were also substantially increased. In contrast, occludin and desmoplakin were suppressed. Knockdown of endogenous CD133 in the Capan1M9 cells led to Slug suppression and reduction of migration and invasion. Taken together, CD133 has an important role in migration and invasion by facilitating EMT in pancreatic cancer cells.
authors: Division of Cancer and Regenerative Medicine, Frontier Science Research Center, Kagoshima University Graduate School of Medical and Dental Sciences, Japan.
authors: Qiang
authors: Q
authors: Ding
authors: Makoto
authors: M
authors: Yoshimitsu
authors: Taisaku
authors: T
authors: Kuwahata
authors: Koki
authors: K
authors: Maeda
authors: Tomomi
authors: T
authors: Hayashi
authors: Toru
authors: T
authors: Obara
authors: Yumi
authors: Y
authors: Miyazaki
authors: Shyuichiro
authors: S
authors: Matsubara
authors: Shoji
authors: S
authors: Natsugoe
authors: Sonshin
authors: S
authors: Takao
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002469:Cell Separation; D004847:Epithelial Cells / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D010455:Peptides / Q000032:analysis*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides