title: Potential biomarkers in mouse myocardium of doxorubicin-induced cardiomyopathy: a metabonomic method and its application.
abstract: BACKGROUND
  Doxorubicin (DOX) is one of the most potent antitumor agents available; however, its clinical use is limited because of the risk of severe cardiotoxicity. Though numerous studies have ascribed DOX cardiomyopathy to specific cellular pathways, the precise mechanism remains obscure. Sini decoction (SND) is a well-known formula of Traditional Chinese Medicine (TCM) and is considered as efficient agents against DOX-induced cardiomyopathy. However, its action mechanisms are not well known due to its complex components.
  
  
  RESULTS
  A tissue-targeted metabonomic method using gas chromatography-mass spectrometry was developed to characterize the metabolic profile of DOX-induced cardiomyopathy in mice. With Elastic Net for classification and selection of biomarkers, twenty-four metabolites corresponding to DOX-induced cardiomyopathy were screened out, primarily involving glycolysis, lipid metabolism, citrate cycle, and some amino acids metabolism. With these altered metabolic pathways as possible drug targets, we systematically analyzed the protective effect of TCM SND, which showed that SND administration could provide satisfactory effect on DOX-induced cardiomyopathy through partially regulating the perturbed metabolic pathways.
  
  
  CONCLUSIONS
  The results of the present study not only gave rise to a systematic view of the development of DOX-induced cardiomyopathy but also provided the theoretical basis to prevent or modify expected damage.
authors: School of Pharmacy, Second Military Medical University, Shanghai, China.
authors: Guangguo
authors: G
authors: Tan
authors: Ziyang
authors: Z
authors: Lou
authors: Wenting
authors: W
authors: Liao
authors: Zhenyu
authors: Z
authors: Zhu
authors: Xin
authors: X
authors: Dong
authors: Wei
authors: W
authors: Zhang
authors: Wuhong
authors: W
authors: Li
authors: Yifeng
authors: Y
authors: Chai
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D009202:Cardiomyopathies / Q000097:blood / Q000139:chemically induced* / Q000378:metabolism*; D004317:Doxorubicin / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D009206:Myocardium / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; C478756:sini tang; D004317:Doxorubicin