title: Specific hypomethylated CpGs at the IGF2 locus act as an epigenetic biomarker for familial adenomatous polyposis colorectal cancer.
abstract: OBJECTIVE
  The identification of specific biomarkers for colorectal cancer is of primary importance for early diagnosis. The aim of this study was to evaluate if methylation changes at the IGF2/H19 locus could be predictive for individuals at high risk for developing sporadic or hereditary colorectal cancer.
  
  
  METHODS
  Quantitative methylation analysis using pyrosequencing was performed on three differentially methylated regions (DMRs): IGF2 DMR0 and DMR2 and the H19 DMR in DNA samples from sporadic colorectal cancer (n = 26), familial adenomatous polyposis (n = 35) and hereditary nonpolyposis colorectal cancer (n = 19) patients.
  
  
  RESULTS
  We report in this article for the first time, that in sporadic colorectal cancer tumor DNA both the IGF2 DMR0 and DMR2 are hypomethylated, while the H19 DMR retains its monoallelic methylation pattern. In lymphocyte DNA, a striking hypomethylation of nine contiguous correlated CpGs was found in the IGF2 DMR2 but only in familial adenomatous polyposis patients.
  
  
  CONCLUSIONS
  Methylation alterations at the IGF2 locus are more extensive than previously reported and DMR2 hypomethylation in lymphocyte DNA might be a specific epigenetic biomarker for familial adenomatous polyposis patients.
authors: Department of Genetics & Development, Institut Cochin, 24, rue Fbg St Jacques, Inserm U567, CNRS UMR 8104, University Paris Descartes, Paris, France.
authors: Audrey
authors: A
authors: Miroglio
authors: Hélène
authors: H
authors: Jammes
authors: Jörg
authors: J
authors: Tost
authors: Loïc
authors: L
authors: Ponger
authors: Ivo Glynne
authors: IG
authors: Gut
authors: Hafida
authors: H
authors: El Abdalaoui
authors: Joël
authors: J
authors: Coste
authors: Stanislas
authors: S
authors: Chaussade
authors: Paola B
authors: PB
authors: Arimondo
authors: Dominique
authors: D
authors: Lamarque
authors: Luisa
authors: L
authors: Dandolo
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000378:metabolism*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D005602:France; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008214:Lymphocytes / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D013447:Sulfites
chemical_list: D015415:Biomarkers; C035170:IGF2 protein, human; D013447:Sulfites; D007335:Insulin-Like Growth Factor II; C042345:hydrogen sulfite