title: Polyphenols from Sophora yunnanensis, and their inhibitory effects on nitric oxide production.
abstract: A new flavonoid, (2R,3R)-3,7,4'-trihydroxy-3',5'-dimethoxyflavanone, named sophorayunnanol (1), together with eight known polyphenols (2-9), were isolated from the roots of Sophora yunnanensis C.Y.MA (Leguminosae). The structure including absolute stereochemistry of 1 was determined by spectroscopic (high resolution (HR)-MS, 1D- and 2D-NMR, and circular dichroism (CD)) methods. The inhibitory activity of these compounds was examined against nitric oxide (NO) production by lipopolysaccharide (LPS) and interferon (IFN)-γ activated macrophages, RAW264.7 cells. 3,7,3',5'-Tetrahydroxy-4'-methoxyflavone (6) and piceatannol (9) showed potent inhibitory activity against the production of NO with IC(50) values of 14.4±2.5 and 12.9±1.8 µM, respectively. This article is the first report on phytochemical study of S. yunnanensis.
authors: School of Pharmacy, Nihon University, Funabashi, Chiba 274–8555, Japan.
authors: Akihiro
authors: A
authors: Daikonya
authors: Susumu
authors: S
authors: Kitanaka
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000276:immunology; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D029909:Sophora / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D008070:Lipopolysaccharides; D059808:Polyphenols; D009569:Nitric Oxide; D007371:Interferon-gamma