title: Isolated compounds from black bean inhibit the classical pathway of the complement.
abstract: The present study evaluated the anticomplement effects from isolated compounds of black bean in classical pathway complement system. Using column chromatograph, four compounds kaempferol 3-O-β-D-glucopyranoside (1), kaempferol 5-O-β-D-glucopyranoside (2), 3-O-[α-L-rhamnopyranosyl(1-2)-β-D-glucopyranosyl(1-2)-β-D-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (3) and 3-O-[β-D-glucopyranosyl(1-2)-β-D-galactopyranosyl(1-2)-β-D-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (4) were isolated and evaluated for in vitro anticomplement activity. 3 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 98.2 μg/ml. This is the first report of anticomplement activity of isolated compounds from black bean.
authors: Department of Medicinal Biotechnology, College of Natural Resources and Life Science, Dong-A University ,  Busan ,  South Korea.
authors: Hyung-In
authors: HI
authors: Moon
authors: Kyoung-Sook
authors: KS
authors: Kim
authors: Young-Choon
authors: YC
authors: Lee
authors: Jai-Heon
authors: JH
authors: Lee