title: 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum.
abstract: BACKGROUND
  There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable.
  
  
  METHODS
  This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH.
  
  
  RESULTS
  With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration). When assayed manually, the between-laboratory variation for six samples surveyed at five laboratories was 3.80-26.4% (CV) for samples containing about 20-290 U/L LIP activity; when assayed using an automated analyzer, the range was 1.86-4.86% (four laboratories). Interference by >5 mmol/L glycerol and low specificity with post-heparin samples were noted, but in practice these are avoidable. Precision analyzed by automated assay of 49 samples twice in random order produced a covariance of 2.27 U/L, which is comparable to routine methods, and good correlations were obtained with five routine methods.
  
  
  CONCLUSIONS
  Although further studies are required, the DODG method may be likely applicable as one candidate reference method.
authors: Tsukuba University Hospital, Ibaraki, Japan.
authors: Yoshiaki
authors: Y
authors: Iizuka
authors: Shigeru
authors: S
authors: Ueda
authors: Toshiro
authors: T
authors: Hanada
authors: Wataru
authors: W
authors: Tani
authors: Hiroshi
authors: H
authors: Adachi
authors: Riichi
authors: R
authors: Haga
authors: Mari
authors: M
authors: Yamaguchi
authors: Wataru
authors: W
authors: Kurotani
authors: Mitsuo
authors: M
authors: Sekiguchi
authors: Dongchon
authors: D
authors: Kang
authors: Shin-ichi
authors: S
authors: Sakasegawa
mesh_terms: D055162:Biocatalysis; D004075:Diglycerides / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008049:Lipase / Q000097:blood* / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*
chemical_list: D004075:Diglycerides; D008049:Lipase; C013965:diolein