title: Tailoring the trajectory of cell rolling with cytotactic surfaces.
abstract: Cell separation technology is a key tool for biological studies and medical diagnostics that relies primarily on chemical labeling to identify particular phenotypes. An emergent method of sorting cells based on differential rolling on chemically patterned substrates holds potential benefits over existing technologies, but the underlying mechanisms being exploited are not well characterized. In order to better understand cell rolling on complex surfaces, a microfluidic device with chemically patterned stripes of the cell adhesion molecule P-selectin was designed. The behavior of HL-60 cells rolling under flow was analyzed using a high-resolution visual tracking system. This behavior was then correlated to a number of established predictive models. The combination of computational modeling and widely available fabrication techniques described herein represents a crucial step toward the successful development of continuous, label-free methods of cell separation based on rolling adhesion.
authors: Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors: Collin
authors: C
authors: Edington
authors: Hironobu
authors: H
authors: Murata
authors: Richard
authors: R
authors: Koepsel
authors: Jill
authors: J
authors: Andersen
authors: Sungeun
authors: S
authors: Eom
authors: Takeo
authors: T
authors: Kanade
authors: Anna C
authors: AC
authors: Balazs
authors: German
authors: G
authors: Kolmakov
authors: Carsen
authors: C
authors: Kline
authors: Daniel
authors: D
authors: McKeel
authors: Zvi
authors: Z
authors: Liron
authors: Alan J
authors: AJ
authors: Russell
mesh_terms: D002234:Carbodiimides / Q000737:chemistry; D002448:Cell Adhesion; D002465:Cell Movement; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D058948:Cell Tracking / Q000379:methods*; D004123:Dimethylamines / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005434:Flow Cytometry; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D019007:P-Selectin / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C505222:11-mercaptoundecanoic acid; D002234:Carbodiimides; D004123:Dimethylamines; D005227:Fatty Acids; C569266:N-ethyl-N'-(3-(dimethylamino)-propyl)carbodiimide; D019007:P-Selectin; D013438:Sulfhydryl Compounds; D006046:Gold