title: Radioprotection of bone marrow hematopoiesis by CpG-oligodeoxynucleotides administered to mice after total-body irradiation.
abstract: CpG-oligodeoxynucleotide (ODN), a synthetic analog of bacteria DNA, has attracted attention because it activates cells of an adaptive immune system and the innate immune system. In this study, we investigated whether CpG-ODN has radioprotective effects, when administered after total-body irradiation (TBI). Mice were treated with 50 µg CpG-ODN via intraperitoneal injection (i.p) within 30 min, 24 h and 48 h after TBI. Our results showed that the survival rate was enhanced at various levels of TBI. The calculated dose reduction factor (DRF) was 1.2. Bone marrow cell count and bone marrow histological examination indicated that CpG-ODN minimized the bone marrow damage induced by TBI. The data of the white blood cell (WBC) count, exogenous (CFU-S) and endogenous (endoCFU-S) colony forming unit-spleen count demonstrated that CpG-ODN reduced primitive hematopoietic stem cells damage and reconstituted hematopoiesis after TBI. Thus, we suggested that CpG-ODN had the potential to contribute to the improvement of the survival rate and limitation of myelosuppression induced by TBI.
authors: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR.
authors: Chao
authors: C
authors: Zhang
authors: Jing
authors: J
authors: Lin
authors: Jianguo
authors: J
authors: Cui
authors: Bailong
authors: B
authors: Li
authors: Cong
authors: C
authors: Liu
authors: Jichao
authors: J
authors: Wang
authors: Fu
authors: F
authors: Gao
authors: Jianming
authors: J
authors: Cai
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000293:injuries / Q000473:pathology / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D014916:Whole-Body Irradiation / Q000009:adverse effects*
chemical_list: C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D011837:Radiation-Protective Agents