title: Electrochemical detection of dihydromyricetin using a DNA immobilized ethylenediamine/polyglutamic modified electrode.
abstract: A novel voltammetric sensor, based on DNA immobilized on the surface of an ethylenediamine/polyglutamic (En/PGA) modified glassy carbon electrode (GCE), was constructed and used for determination of dihydromyricetin (DMY). The electrochemical behaviour of DMY at this sensor was investigated in pH 3.6 NaAc-HAc buffer solutions by cyclic voltammetry (CV) and differential pulse anodic voltammetry (DPV). The oxidation of DMY is an adsorption-controlled irreversible process. The oxidation mechanism was proposed and discussed. It was found that the modified electrode exhibited a linear voltammetric response for DMY in the range of 4.0 × 10(-8) mol L(-1) to 2 × 10(-6) mol L(-1), with a detection limit of 2 × 10(-8) mol L(-1). The method was also applied successfully to detect DMY in an ampelopsis sample with satisfactory results.
authors: Department of Chemistry, Zhengzhou University, Zhengzhou, 450001, P R China.
authors: Lina
authors: L
authors: Zou
authors: Ying
authors: Y
authors: Xu
authors: Peili
authors: P
authors: Luo
authors: Shusheng
authors: S
authors: Zhang
authors: Baoxian
authors: B
authors: Ye
mesh_terms: D032421:Ampelopsis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D005029:Ethylenediamines / Q000737:chemistry* / Q000378:metabolism; D044948:Flavonols / Q000032:analysis*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005029:Ethylenediamines; D044948:Flavonols; D011099:Polyglutamic Acid; C031234:ethylenediamine; D004247:DNA; C472036:dihydromyricetin