title: Arsenate induces the expression of fungal genes involved in As transport in arbuscular mycorrhiza.
abstract: We utilized the two-compartment system to study the effect of arsenic (As) on the expression of the Glomus intraradices high-affinity phosphate transporter GiPT, and the GiArsA gene, a novel protein with a possible putative role as part of an arsenite efflux pump and similar to ArsA ATPase. Our results show that induction of GiPT expression correlates with As(V) uptake in the extra-radical mycelium of G. intraradices. We showed a time-concerted induction of transcript levels first of GiPT, followed by GiArsA, as well as the location of gene expression using laser microdissection of these two genes not only in the extra-radical mycelium but also in arbuscules. This work represents the first report showing the dissection of the molecular players involved in arbuscular mycorrhizal fungus (AMF)-mediated As tolerance in plants, and suggests that tolerance mediated by AMF may be caused by an As exclusion mechanism, where fungal structures such as the extra-radical mycelium and arbuscules may be playing an important role. Our results extend knowledge of the mechanisms underlying As efflux in arbuscular mycorrhizal fungi and mechanisms related to As tolerance.
authors: Programa de Edafología, Instituto de Recursos Naturales, Colegio de Postgraduados en Ciencias Agrícolas, Campus Montecillo, Carretera México-Texcoco Km 36.5, Texcoco, México 56230, Mexico.
authors: Ma del Carmen A
authors: Mdel C
authors: González-Chávez
authors: María del Pilar
authors: Mdel P
authors: Ortega-Larrocea
authors: Rogelio
authors: R
authors: Carrillo-González
authors: Melina
authors: M
authors: López-Meyer
authors: Beatriz
authors: B
authors: Xoconostle-Cázares
authors: Susana K
authors: SK
authors: Gomez
authors: Maria J
authors: MJ
authors: Harrison
authors: Alejandro Miguel
authors: AM
authors: Figueroa-López
authors: Ignacio E
authors: IE
authors: Maldonado-Mendoza
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001149:Arsenates / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D055137:Glomeromycota / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny
chemical_list: D001149:Arsenates; D005656:Fungal Proteins; D028061:Phosphate Transport Proteins; D000251:Adenosine Triphosphatases; C025657:arsenic acid