title: Two classes of glutathione S-transferase genes with different response profiles to bacterial challenge in Venerupis philippinarum.
abstract: Glutathione S-transferase (GST) is major cytosolic detoxification enzymes involved in many pathological and physiological processes. In the present study, two classes of GSTs (VpGST-1 and VpGST-2) were cloned from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. Sequence alignments and phylogenetic analysis together supported that they belonged to a new member of sigma and pi classes GSTs protein family, respectively. The expression profiles of these two genes under Vibrio anguillarum challenge were investigated by quantitative RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression of both VpGST-1 and VpGST-2 with larger amplitude in VpGST-2, and the feedback speed for VpGST-2 was more rapid than that of VpGST-1. The differences in the response to bacterial challenge indicated that they were functional diversity and probably played cooperative roles in mediating the Vibrio challenge in clam.
authors: Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211, PR China. chli@yic.ac.cn
authors: Chenghua
authors: C
authors: Li
authors: Xiurong
authors: X
authors: Su
authors: Ye
authors: Y
authors: Li
authors: Taiwu
authors: T
authors: Li
authors: Chongjie
authors: C
authors: Sun
authors: Tingting
authors: T
authors: Zhou
authors: Haipeng
authors: H
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014733:Vibrio / Q000502:physiology*
chemical_list: D005982:Glutathione Transferase