title: Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae).
abstract: OBJECTIVE
  We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan.
  
  
  RESULTS
  mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species' entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively.
  
  
  CONCLUSIONS
  The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.
authors: Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Japan. sakaguci@kais.kyoto-u.ac.jp
authors: Shota
authors: S
authors: Sakaguchi
authors: Kentaro
authors: K
authors: Uchiyama
authors: Saneyoshi
authors: S
authors: Ueno
authors: Tokuko
authors: T
authors: Ujino-Ihara
authors: Yoshihiko
authors: Y
authors: Tsumura
authors: Lynda D
authors: LD
authors: Prior
authors: David M J S
authors: DM
authors: Bowman
authors: Michael D
authors: MD
authors: Crisp
authors: Yuji
authors: Y
authors: Isagi
mesh_terms: D000483:Alleles; D001315:Australia; D029779:Cupressaceae / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D020224:Expressed Sequence Tags; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D005843:Geography; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers