title: Overexpression or downregulation of mTOR in mammalian cells.
abstract: This chapter presents an overview of the methods that have been used to overexpress or downregulate the level of mTOR isoforms in mammalian cells. The techniques of transient overexpression, generation of stable cell lines, retroviral- and lentiviral-mediated overexpression or downregulation are discussed.
authors: Department of Structural and Molecular Biology, Institute of Structural and Molecular Biology, University College London, London, UK.
authors: Mahmoud
authors: M
authors: Khalil
authors: Ivan
authors: I
authors: Gout
mesh_terms: D000818:Animals; D002460:Cell Line; D015536:Down-Regulation; D018274:Electroporation; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D000076222:Mechanistic Target of Rapamycin Complex 1; D051379:Mice; D046912:Multiprotein Complexes; D041681:NIH 3T3 Cells; D011506:Proteins / Q000235:genetics*; D058570:TOR Serine-Threonine Kinases; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: C493245:CRTC2 protein, human; D046912:Multiprotein Complexes; D011506:Proteins; D014157:Transcription Factors; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases