title: The role of HE4 in ovarian cancer: inhibiting tumour cell proliferation and metastasis.
abstract: As a promising biomarker, human epididymis protein 4 (HE4) has been widely used for the early detection and differential diagnosis of ovarian cancer. This study evaluated the function of HE4 in the carcinogenesis and progression of ovarian cancer. An enzyme immunometric assay, used  to detect HE4 in the serum of ovarian cancer patients, showed that the protein could discriminate between malignant and benign ovarian tumours with high specificity. An exogenous HE4 gene was transfected into ovarian cancer cell lines and an immortalized ovarian epithelial cell line.  Compared with the controls, HE4 overexpression significantly promoted cell apoptosis and adhesion. Overexpression of HE4 also led to significant inhibition of cell proliferation, migration and invasiveness in vitro, as well as xenograft tumour formation in vivo. This is the first  report to demonstrate the functional importance of HE4 in multiple cellular processes and indicates that HE4 may play a protective role in the progression of ovarian cancer.
authors: Gynecological Oncology Centre, Peking University People's Hospital, Beijing, China.
authors: L
authors: L
authors: Gao
authors: H Y
authors: HY
authors: Cheng
authors: L
authors: L
authors: Dong
authors: X
authors: X
authors: Ye
authors: Y N
authors: YN
authors: Liu
authors: X H
authors: XH
authors: Chang
authors: Y X
authors: YX
authors: Cheng
authors: J
authors: J
authors: Chen
authors: R Q
authors: RQ
authors: Ma
authors: H
authors: H
authors: Cui
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000097:blood* / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden; D000081102:WAP Four-Disulfide Core Domain Protein 2
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; D000081102:WAP Four-Disulfide Core Domain Protein 2; C569698:WFDC2 protein, human