title: No evidence for XMRV nucleic acids, infectious virus or anti-XMRV antibodies in Canadian patients with chronic fatigue syndrome.
abstract: The gammaretroviruses xenotropic murine leukemia virus (MLV)-related virus (XMRV) and MLV have been reported to be more prevalent in plasma and peripheral blood mononuclear cells of chronic fatigue syndrome (CFS) patients than in healthy controls. Here, we report the complex analysis of whole blood and plasma samples from 58 CFS patients and 57 controls from Canada for the presence of XMRV/MLV nucleic acids, infectious virus, and XMRV/MLV-specific antibodies. Multiple techniques were employed, including nested and qRT-PCR, cell culture, and immunoblotting. We found no evidence of XMRV or MLV in humans and conclude that CFS is not associated with these gammaretroviruses.
authors: Blood Systems Research Institute, San Francisco, California, United States of America.
authors: Imke
authors: I
authors: Steffen
authors: D Lorne
authors: DL
authors: Tyrrell
authors: Eleanor
authors: E
authors: Stein
authors: Leilani
authors: L
authors: Montalvo
authors: Tzong-Hae
authors: TH
authors: Lee
authors: Yanchen
authors: Y
authors: Zhou
authors: Kai
authors: K
authors: Lu
authors: William M
authors: WM
authors: Switzer
authors: Shaohua
authors: S
authors: Tang
authors: Hongwei
authors: H
authors: Jia
authors: Darren
authors: D
authors: Hockman
authors: Deanna M
authors: DM
authors: Santer
authors: Michael
authors: M
authors: Logan
authors: Amir
authors: A
authors: Landi
authors: John
authors: J
authors: Law
authors: Michael
authors: M
authors: Houghton
authors: Graham
authors: G
authors: Simmons
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002170:Canada; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics*; D015673:Fatigue Syndrome, Chronic / Q000097:blood / Q000276:immunology* / Q000821:virology*; D005260:Female; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012192:Retroviridae Infections / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000821:virology*; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D004279:DNA, Viral; D012333:RNA, Messenger