title: Activation of the aryl hydrocarbon receptor represses mammosphere formation in MCF-7 cells.
abstract: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor. Recent studies have reported the anti-tumor effects of the AhR in breast cancer. In this study, we investigated the anti-tumor effect of AhR activation based on the cancer stem cell hypothesis. We show that AhR activation suppressed mammosphere formation of MCF-7 cells and decreased the proportion of cells with high ALDH-1 (aldehyde dehydrogenase 1) activity. In addition, we also demonstrate that AhR activation regulates self-renewal signaling by down-regulating Wnt/β-catenin and Notch.
authors: Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
authors: Shuai
authors: S
authors: Zhao
authors: Yuichiro
authors: Y
authors: Kanno
authors: Momoka
authors: M
authors: Nakayama
authors: Minami
authors: M
authors: Makimura
authors: Shiori
authors: S
authors: Ohara
authors: Yoshio
authors: Y
authors: Inouye
mesh_terms: D000080924:Aldehyde Dehydrogenase 1 Family; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008748:Methylcholanthrene / Q000494:pharmacology; D018613:Microscopy, Confocal; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000235:genetics* / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism; D050697:Retinal Dehydrogenase / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism; D019324:beta-Naphthoflavone / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D018336:Receptors, Aryl Hydrocarbon; D051880:Receptors, Notch; D051153:Wnt Proteins; D051176:beta Catenin; D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase