title: The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development.
abstract: Sox17 is a HMG-box transcription factor that has been shown to play important roles in both cardio-vascular development and endoderm formation. To analyze these processes in greater detail, we have generated a Sox17-mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are homozygous viable and faithfully reflect the endogenous Sox17 protein localization. We report that SCF positive cells constitute a subpopulation in the visceral endoderm before gastrulation and time-lapse imaging reveals that SCF monitors the nascent definitive endoderm during epithelialization. After gastrulation, SCF marks the mid- and hindgut endoderm and vascular endothelial network, which can be imaged during establishment in allantois explant cultures. The SCF reporter is downregulated in the endoderm epithelium and upregulated in endothelial cells of the intestine, lung, and pancreas during organogenesis. In summary, the generation of the Sox17-mCherry reporter mouse line allows direct visualization of endoderm and vascular development in culture and the mouse embryo.
authors: Institute of Diabetes and Regeneration, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany.
authors: Ingo
authors: I
authors: Burtscher
authors: Wenke
authors: W
authors: Barkey
authors: Michael
authors: M
authors: Schwarzfischer
authors: Fabian J
authors: FJ
authors: Theis
authors: Heiko
authors: H
authors: Lickert
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D053595:Embryonic Stem Cells; D004707:Endoderm / Q000196:embryology; D004730:Endothelium, Vascular / Q000196:embryology*; D018703:Founder Effect; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D005838:Genotype; D024002:HMGB Proteins / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics*; D051379:Mice; D011993:Recombinant Fusion Proteins; D055760:SOXF Transcription Factors / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D024002:HMGB Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D055760:SOXF Transcription Factors; D000097573:Red Fluorescent Protein; C511606:Sox17 protein, mouse