title: Alloantibodies to human platelet glycoprotein antigens (HPA) and HLA class 1 in a cross section of Nigerian antenatal women.
abstract: The prevalence of antibodies to human platelet antigens (HPA) and human leukocyte antigens (HLA) class 1 antigens among Nigerian pregnant women has not been reported in our country. This study was therefore aimed at screening the obstetric population for evidence of alloimmunization due to human platelet and HLA class 1 antigens. One hundred and forty four (144) pregnant women attending the obstetric clinic of Military Hospital, Port Harcourt, participated in the study. Their sera were tested for antibodies to HPA and HLA class 1 antigens using GTI PakPlus solid phase ELISA Kit. The total prevalence rate of antibody production was 60.5% (87 out of 144). Among the positive samples, 60 had platelet glycoprotein specific antibodies (41.7%) and 27 had HLA class 1 antibodies (18.8%). In 39.6% of the pregnant women, both platelet specific antibodies and HLA class 1 antibodies appeared. The prevalence of platelet specific glycoprotein antibodies were obtained as follows: GP 11b/111a 12 (8.3%), GP 1a/11a 35 (20.8%), GP Ib/IX 18 (12.5%) and GP IV 9 (6.3%). The prevalence of each platelet antibody subgroup was obtained as follows: anti-HPA-1a,-3a,-4a (4.2%), anti-HPA-1b,-3b,-4a (4.2%), anti-HPA-30 5a and anti-GP Ib/IX (12.5% each), anti-HPA-5b (8.3%) and anti-GP IV (6.3%). A high prevalence rate of human platelet arid cytotoxic antibodies has been observed in our obstetric population. There is need to establish platelet serology laboratory for the proper antenatal and postnatal management of pregnant mothers in this region.
authors: Haematology and Blood Transfusion Science Unit, Department of Medical Laboratory Sciences College of Health Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria. zacjerry@yahoo.com
authors: Zaccheaus Awortu
authors: ZA
authors: Jeremiah
authors: Anne Ifeanyi
authors: AI
authors: Atiegoba
authors: Osaro
authors: O
authors: Mgbere
mesh_terms: D000328:Adult; D016824:Antigens, Human Platelet / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000150:complications / Q000453:epidemiology / Q000276:immunology*; D001792:Blood Platelets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005333:Fetus; D015788:HLA-A1 Antigen / Q000097:blood* / Q000276:immunology; D006772:Hospitals, Military; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000097:blood / Q000276:immunology; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D009549:Nigeria / Q000453:epidemiology; D011247:Pregnancy; D015995:Prevalence; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000209:etiology / Q000276:immunology
chemical_list: D016824:Antigens, Human Platelet; D015788:HLA-A1 Antigen; D007126:Immunoglobulin Allotypes; D007518:Isoantibodies