title: [Tissue culture and polyploidy induction of Morinda officinalis].
abstract: OBJECTIVE
  To establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis.
  
  
  METHODS
  Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry.
  
  
  CONCLUSIONS
  The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.
authors: Department of Pharmacy, Zhangzhou Health Vocational College, Zhangzhou 363000, China. lmz575@163.com
authors: Meizhen
authors: M
authors: Lin
authors: Qingzhen
authors: Q
authors: Wu
authors: Song
authors: S
authors: Zheng
authors: Huiqiao
authors: H
authors: Tian
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D032066:Morinda / Q000235:genetics* / Q000254:growth & development*; D011123:Polyploidy; D046509:Tissue Culture Techniques / Q000379:methods*