title: The proteome of normal pancreatic juice.
abstract: OBJECTIVE
  The aims of this study were to characterize the proteome of normal pancreatic juice, to analyze the effect of secretin on the normal proteome, and to compare these results with published data from patients with pancreatic cancer.
  
  
  METHODS
  Paired pancreatic fluid specimens (before and after intravenous secretin stimulation) were obtained during endoscopic pancreatography from 3 patients without significant pancreatic pathology. Proteins were identified and quantified by mass spectrometry-based protein quantification technology. The human RefSeq (NCBI) database was used to compare the data in samples from patients without pancreatic disease with published data from 3 patients with pancreatic cancer.
  
  
  RESULTS
  A total of 285 proteins were identified in normal pancreatic juice. Ninety had sufficient amino acid sequences identified to characterize the protein with a high level of confidence. All 90 proteins were present before and after secretin administration but with altered relative concentrations, usually by 1 to 2 folds, after stimulation. Comparison with 170 published pancreatic cancer proteins yielded an overlap of only 42 proteins.
  
  
  CONCLUSIONS
  Normal pancreatic juice contains multiple proteins related to many biological processes. Secretin alters the concentration but not the spectrum of these proteins. The pancreatic juice proteome of patients without pancreatic disease and that of patients with pancreatic cancer differ markedly.
authors: Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
authors: Courtney J
authors: CJ
authors: Doyle
authors: Kyle
authors: K
authors: Yancey
authors: Henry A
authors: HA
authors: Pitt
authors: Mu
authors: M
authors: Wang
authors: Kerry
authors: K
authors: Bemis
authors: Michele T
authors: MT
authors: Yip-Schneider
authors: Stuart T
authors: ST
authors: Sherman
authors: Keith D
authors: KD
authors: Lillemoe
authors: Michael D
authors: MD
authors: Goggins
authors: C Max
authors: CM
authors: Schmidt
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D002760:Cholangiopancreatography, Endoscopic Retrograde; D002853:Chromatography, Liquid; D019295:Computational Biology; D005260:Female; D006801:Humans; D007196:Indiana; D010182:Pancreatic Diseases / Q000378:metabolism*; D010189:Pancreatic Juice / Q000737:chemistry* / Q000187:drug effects; D010190:Pancreatic Neoplasms / Q000737:chemistry*; D011237:Predictive Value of Tests; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012633:Secretin / Q000008:administration & dosage; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; D012633:Secretin