title: Tension-compression loading with chemical stimulation results in additive increases to functional properties of anatomic meniscal constructs.
abstract: OBJECTIVE
  This study aimed to improve the functional properties of anatomically-shaped meniscus constructs through simultaneous tension and compression mechanical stimulation in conjunction with chemical stimulation.
  
  
  METHODS
  Scaffoldless meniscal constructs were subjected to simultaneous tension and compressive stimulation and chemical stimulation. The temporal aspect of mechanical loading was studied by employing two separate five day stimulation periods. Chemical stimulation consisted of the application of a catabolic GAG-depleting enzyme, chondroitinase ABC (C-ABC), and an anabolic growth factor, TGF-β1. Mechanical and chemical stimulation combinations were studied through a full-factorial experimental design and assessed for histological, biochemical, and biomechanical properties following 4 wks of culture.
  
  
  RESULTS
  Mechanical loading applied from days 10-14 resulted in significant increases in compressive, tensile, and biochemical properties of meniscal constructs. When mechanical and chemical stimuli were combined significant additive increases in collagen per wet weight (4-fold), compressive instantaneous (3-fold) and relaxation (2-fold) moduli, and tensile moduli in the circumferential (4-fold) and radial (6-fold) directions were obtained.
  
  
  CONCLUSIONS
  This study demonstrates that a stimulation regimen of simultaneous tension and compression mechanical stimulation, C-ABC, and TGF-β1 is able to create anatomic meniscus constructs replicating the compressive mechanical properties, and collagen and GAG content of native tissue. In addition, this study significantly advances meniscus tissue engineering by being the first to apply simultaneous tension and compression mechanical stimulation and observe enhancement of tensile and compressive properties following mechanical stimulation.
authors: Department of Biomedical Engineering, University of California Davis, Davis, California, United States of America.
authors: Daniel J
authors: DJ
authors: Huey
authors: Kyriacos A
authors: KA
authors: Athanasiou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D002478:Cells, Cultured; D019765:Chondroitin ABC Lyase / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D019245:Compressive Strength; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008592:Menisci, Tibial / Q000187:drug effects* / Q000502:physiology; D013268:Stimulation, Chemical; D013718:Tensile Strength; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D053773:Transforming Growth Factor beta1; D003094:Collagen; D019765:Chondroitin ABC Lyase