title: Coating and release of an anti-inflammatory hormone from PLGA microspheres for tissue engineering.
abstract: Many biomaterials used in tissue engineering cause a foreign body response in vivo, which left untreated can severely reduce the effectiveness of tissue regeneration. In this study, an anti-inflammatory hormone α-melanocyte stimulating hormone (α-MSH) was physically adsorbed to the surface of biodegradable poly (lactic-co-glycolic) acid (PLGA) microspheres to reduce inflammatory responses to this material. The stability and adsorption isotherm of peptide binding were characterized. The peptide secondary structure was not perturbed by the adsorption and subsequent desorption process. The α-MSH payload was released over 72 h and reduced the expression of the inflammatory cytokine, Tumor necrosis factor-α (TNF-α) in lipopolysaccharide activated RAW 264.7 macrophage cells, indicating that the biological activity of α-MSH was preserved. α-MSH coated PLGA microspheres also appeared to reduce the influx of inflammatory cells in a subcutaneous implantation model in rats. This study demonstrates the potential of α-MSH coatings for anti-inflammatory delivery and this approach may be applied to other tissue engineering applications.
authors: Department of Chemical and Biomolecular Engineering, Particulate Fluids Processing Centre, University of Melbourne, Parkville 3010, Victoria, Australia; Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville 3010, Victoria, Australia.
authors: Dewi P
authors: DP
authors: Go
authors: Jason A
authors: JA
authors: Palmer
authors: Sally L
authors: SL
authors: Gras
authors: Andrea J
authors: AJ
authors: O'Connor
mesh_terms: D000327:Adsorption; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002942:Circular Dichroism; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D051379:Mice; D008863:Microspheres; D009195:Peroxidase / Q000378:metabolism; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary; D058525:Quartz Crystal Microbalance Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D000521:alpha-MSH / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D014409:Tumor Necrosis Factor-alpha; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000521:alpha-MSH; D009195:Peroxidase