title: Association analysis between the -2518MCP-1(A/G) polymorphism and generalized aggressive periodontitis in a Chinese population.
abstract: OBJECTIVE
  It has been suggested that aggressive periodontitis has a genetic basis. Monocyte chemoattractant protein 1 (MCP-1) plays a critical role in the recruitment of monocytes and the development of periodontitis. The -2518MCP-1(A/G) polymorphism has been implicated as a risk or susceptibility factor for a variety of autoimmune conditions and inflammatory diseases. The intent of this investigation was to study whether the -2518MCP-1(A/G) polymorphism is associated with generalized aggressive periodontitis in the Chinese population.
  
  
  METHODS
  One hundred and twenty-four patients with generalized aggressive periodontitis and 94 healthy subjects were included in this case-control study. Genomic DNA was isolated from a peripheral blood sample obtained from each subject. Gene polymorphisms of -2518MCP-1(A/G) were analyzed by a standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. A logistic regression analysis was performed to test the association between the -2518MCP-1(A/G) genotype (alleles) and generalized aggressive periodontitis with adjustment of the major covariates (gender, age and smoking status).
  
  
  RESULTS
  There was no significant association of the -2518MCP-1(A/G) polymorphism with generalized aggressive periodontitis in the unstratified subjects. However, when patients were stratified by gender, the frequency of the G(+) genotype was significantly lower in female patients with generalized aggressive periodontitis compared with female controls (p = 0.036, adjusted odds ratio = 0.3, 95% CI: 0.1-0.9). In female patients with generalized aggressive periodontitis, the probing pocket depth was larger in subjects with the AA genotype than in subjects with the G(+) genotype (5.07 mm vs. 4.30 mm; Z = -2.470, p = 0.014).
  
  
  CONCLUSIONS
  The polymorphisms of -2518MCP-1 may play an important role in determining generalized aggressive periodontitis susceptibility in this cohort of Chinese women.
authors: Department of Periodontology, Peking University, School and Hospital of Stomatology, Beijing, China.
authors: X L
authors: XL
authors: Zhu
authors: H X
authors: HX
authors: Meng
authors: L
authors: L
authors: Zhang
authors: L
authors: L
authors: Xu
authors: Z B
authors: ZB
authors: Chen
authors: D
authors: D
authors: Shi
authors: X H
authors: XH
authors: Feng
authors: X
authors: X
authors: Zhang
mesh_terms: D000225:Adenine; D000328:Adult; D010520:Aggressive Periodontitis / Q000145:classification / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000097:blood / Q000235:genetics*; D002681:China; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006147:Guanine; D006801:Humans; D008297:Male; D017622:Periodontal Attachment Loss / Q000145:classification / Q000235:genetics; D010514:Periodontal Pocket / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D006147:Guanine; D000225:Adenine