title: Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia.
abstract: BACKGROUND
  The purpose here was to detect the association among plasma matrix metalloproteinase-9 (MMP-9) concentration, single nucleotide polymorphisms (SNPs) of MMP-9 gene and community-acquired pneumonia (CAP).
  
  
  METHODS
  The enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were, respectively used to measure the plasma MMP-9 level and its gene polymorphisms.
  
  
  RESULTS
  The level of plasma of MMP-9 was elevated in patients with CAP as compared to that of normal controls and decreased significantly after treatment. Plasma MMP-9 concentration was significantly correlated with white blood cell (WBC) and neutrophil counts in patients with CAP. No significant difference was found in the genotypes distribution of MMP-9 SNPs, rs3918242, rs17576 or rs2274756, between patients with CAP and normal controls. Plasma MMP-9 concentration was not associated with MMP-9 polymorphism. When the cut-off level of the plasma MMP-9 concentration was set to be 105.02 ng/mL, the odds ratio of plasma MMP-9 for CAP risk was 9.86 (95% confident interval: 4.27-22.75). Plasma MMP-9 level may act as a prediction marker for CAP.
  
  
  CONCLUSIONS
  Elevated plasma MMP-9 could be a biological marker for the diagnosis and be a new strategy for target therapy of community-acquired pneumonia.
authors: School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors: Ting-Yen
authors: TY
authors: Chiang
authors: Ling-Yuh
authors: LY
authors: Shyu
authors: Thomas-Chang Yao
authors: TC
authors: Tsao
authors: Ming-Hsien
authors: MH
authors: Chien
authors: Shih-Ming
authors: SM
authors: Tsao
authors: Yuan-Ti
authors: YT
authors: Lee
authors: Shun-Fa
authors: SF
authors: Yang
mesh_terms: D016022:Case-Control Studies; D017714:Community-Acquired Infections / Q000097:blood / Q000201:enzymology / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology; D011014:Pneumonia / Q000097:blood* / Q000201:enzymology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D020780:Matrix Metalloproteinase 9