title: [Determination of main peptide toxins from Amanita pallidorosea with HPLC and their antifungal action on Blastomyces albicans].
abstract: OBJECTIVE
  To detect peptide toxins in Amanita pallidorasea and to study the antifungal activities of peptide toxins against Blastomyces albicans.
  
  
  METHODS
  We separated and identified peptide toxins and determined its contents in the fruiting body, pileus and the mixture of stipe and volva from A. pallidorasea by HPLC and ESI-MS methods. Meanwhile, we detected antifungal activities of the crude toxin and the separated peptide toxins against Blastomyces albicans JLC31680 and JLC31681 by the paper disk method.
  
  
  RESULTS
  We totally got three peptide toxins: alpha-amanitin (alpha-AMA), beta-amanitin (beta-AMA) and phalloidin (PHD). The contents of alpha-AMA, beta-AMA and PHD were 30.3 mg/g, 6.99 mg/g and 9.95 mg/g in fruiting body, and 45.0 mg/g, 11.1 mg/g and 11.3 mg/g in pileus. The contents of alpha-AMA and PHD were 11.7 mg/g and 7.98 mg/g in the mixture of stipe and volva , but the beta-AMA was not detected in this part. The inhibition ratio of the crude toxin and alpha-AMA, beta-AMA and PHD to B. albicans JLC31680 were 11.96%, 32.52%, 23.29% (P<0.01) and 15.46% (P<0.05). The inhibition ratio of the crude toxin and beta-AMA to B. albicans JLC31681 was 10.16% and 11.10% (P < 0.01), while that of alpha-AMA's was 6.89% (P < 0.05).
  
  
  CONCLUSIONS
  A. pallidorasea is a new resource of peptide toxins with antifungal activity.
authors: Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
authors: Yuling
authors: Y
authors: Wang
authors: Haiying
authors: H
authors: Bao
authors: Lu
authors: L
authors: Xu
authors: Tolgor
authors: T
authors: Bau
mesh_terms: D000545:Amanita / Q000378:metabolism*; D000546:Amanitins / Q000032:analysis / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001758:Blastomyces / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D005656:Fungal Proteins / Q000032:analysis* / Q000494:pharmacology*; D009183:Mycotoxins / Q000032:analysis* / Q000494:pharmacology*; D010590:Phalloidine / Q000032:analysis / Q000494:pharmacology
chemical_list: D000546:Amanitins; D000935:Antifungal Agents; D005656:Fungal Proteins; D009183:Mycotoxins; D010590:Phalloidine