title: Suppression of lamin A/C by short hairpin RNAs promotes adipocyte lineage commitment in mesenchymal progenitor cell line, ROB-C26.
abstract: Lamin A/C gene encodes a nuclear membrane protein, and mutations in this gene are associated with diverse degenerative diseases that are linked to premature aging. While lamin A/C is involved in the regulation of tissue homeostasis, the distinct expression patterns are poorly understood in the mesenchymal cells differentiating into adipocytes. Here, we examined the expression of lamin A/C in a rat mesenchymal progenitor cell-line, ROB-C26 (C26). Immunocytochemical analysis showed that lamin A/C was transiently down-regulated in immature adipocytes, but its expression increased with terminal differentiation. To elucidate the role of lamin A/C expression on mesenchymal cell differentiation, lamin A/C expression was suppressed using short hairpin RNA (shRNA) molecules in C26 cells. In the absence of adipogenic stimuli, lamin A/C shRNA decreased alkaline phosphatase (ALP) activity, but induced preadipocyte factor -1 (Pref-1) mRNA expression. In the presence of adipogenic stimuli, lamin A/C knockdown promotes adipocytes differentiation, as assessed by the detection of an increase in Oil Red O staining. RT-PCR analysis showed that lamin A/C shRNA resulted in increased mRNA expression of PPARγ2 and aP2 during adipocyte differentiation. These results suggest that decreased lamin A/C expression levels not only suppress osteoblast phenotypes but also promote adipocyte differentiation in C26 cells.
authors: Department of Anatomy, Nihon University School of Dentistry, Kanda-Surugadai, Chiyodaku, Tokyo, Japan. oomura@dent.nihon-u.ac.jp
authors: Masako
authors: M
authors: Naito
authors: Kazuki
authors: K
authors: Omoteyama
authors: Yoshikazu
authors: Y
authors: Mikami
authors: Minoru
authors: M
authors: Takagi
authors: Tomihisa
authors: T
authors: Takahashi
mesh_terms: D033962:Adaptor Protein Complex 2 / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D005260:Female; D055785:Gene Knockdown Techniques; D034904:Lamin Type A / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D034881:Nuclear Lamina / Q000378:metabolism; D010006:Osteoblasts; D047495:PPAR gamma / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D013234:Stem Cells / Q000378:metabolism
chemical_list: D033962:Adaptor Protein Complex 2; D034904:Lamin Type A; D047495:PPAR gamma; D034741:RNA, Small Interfering; C440308:lamin C; D000469:Alkaline Phosphatase