title: In vivo intra-luteal implants of prostaglandin (PG) E1 or E2 (PGE1, PGE2) prevent luteolysis in cows. II: mRNA for PGF2α, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4 prostanoid receptors in luteal tissue.
abstract: Previously, it was reported that chronic intra-uterine infusion of PGE(1) or PGE(2) every 4h inhibited luteolysis in ewes by altering luteal mRNA for luteinizing hormone (LH) receptors and unoccupied and occupied luteal LH receptors. However, estradiol-17β or PGE(2) given intra-uterine every 8h did not inhibit luteolysis in cows, but infusion of estradiol+PGE(2) inhibited luteolysis. In contrast, intra-luteal implants containing PGE(1) or PGE(2) in Angus or Brahman cows also inhibited the decline in circulating progesterone, mRNA for LH receptors, and loss of unoccupied and occupied receptors for LH to prevent luteolysis. The objective of this experiment was to determine how intra-luteal implants of PGE(1) or PGE(2) alter mRNA for prostanoid receptors and how this could influence luteolysis in Brahman or Angus cows. On day-13 Angus cows received no intra-luteal implant and corpora lutea were retrieved or Angus and Brahman cows received intra-luteal silastic implants containing Vehicle, PGE(1), or PGE(2) and corpora lutea were retrieved on day-19. Corpora lutea slices were analyzed for mRNA for prostanoid receptors (FP, EP1, EP2, EP3 (A-D), EP3A, EP3B, EP3C, EP3D, and EP4) by RT-PCR. Day-13 Angus cow luteal tissue served as pre-luteolytic controls. mRNA for FP receptors decreased in day-19 Vehicle controls compared to day-13 Vehicle controls regardless of breed. PGE(1) and PGE(2) up-regulated FP gene expression on day-19 compared to day-19 Vehicle controls regardless of breed. EP1 mRNA was not altered by any treatment. PGE(1) and PGE(2) down-regulated EP2 and EP4 mRNA compared to day-19 Vehicle controls regardless of breed. PGE(1) or PGE(2) up-regulated mRNA EP3B receptor subtype compared to day-19 Vehicle control cows regardless of breed. The similarities in relative gene expression profiles induced by PGE(1) and PGE(2) support their agonistic effects. We conclude that both PGE(1) and PGE(2) may prevent luteolysis by altering expression of mRNA for prostanoid receptors, which is correlated with changes in luteal mRNA for LH receptors reported previously in these same cows to prevent luteolysis.
authors: Department of HNFAS, University of Hawaii, Honolulu, USA.
authors: Yoshie S
authors: YS
authors: Weems
authors: Phillip J
authors: PJ
authors: Bridges
authors: Myoungkun
authors: M
authors: Jeoung
authors: J Alejandro
authors: JA
authors: Arreguin-Arevalo
authors: Torrance M
authors: TM
authors: Nett
authors: Rhonda C
authors: RC
authors: Vann
authors: Stephen P
authors: SP
authors: Ford
authors: Andrew W
authors: AW
authors: Lewis
authors: Don A
authors: DA
authors: Neuendorff
authors: Thomas H
authors: TH
authors: Welsh
authors: Ronald D
authors: RD
authors: Randel
authors: Charles W
authors: CW
authors: Weems
mesh_terms: D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D003341:Luteolysis / Q000187:drug effects* / Q000235:genetics; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000235:genetics*; D058306:Receptors, Prostaglandin E, EP1 Subtype / Q000235:genetics; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000235:genetics; D058308:Receptors, Prostaglandin E, EP3 Subtype / Q000235:genetics; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; D058306:Receptors, Prostaglandin E, EP1 Subtype; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058308:Receptors, Prostaglandin E, EP3 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; C034671:prostaglandin F2alpha receptor; D000527:Alprostadil; D015232:Dinoprostone