title: Radioligand-binding assay reveals distinct autoantibody preferences for type I interferons in APS I and myasthenia gravis subgroups.
abstract: Patients with autoimmune polyendocrine syndrome type I (APS I) or acquired thymoma-associated myasthenia gravis (MG) surprisingly share several common features, including defective expression of the transcription factor AIRE and autoantibodies against type I interferons. Here, we have adapted and validated the radioligand-binding assay we recently developed against (35)S-Met-interferon-ω, for rapid and specific screening for autoantibodies against interferons-α2 and -α8. We then investigated their potential for diagnosis and for predicting clinical manifestations in patients with APS I and different subgroups of MG. Autoantibodies against interferons-ω, -α2, and -α8 occurred more often in patients with APS I (100%) and MG with thymoma (73%) than in late-onset MG (39%) and early-onset MG (5%). These autoantibodies showed preferences for interferon-ω in APS I and for the interferon-αs in MG, hinting at thymic aberrations in both groups. The exact profile of type I interferon antibodies may indicate MG subtype and may hint at thymoma recurrence.
authors: Institute of Medicine, University of Bergen, 5021 Bergen, Norway.
authors: Liv
authors: L
authors: Hapnes
authors: Nick
authors: N
authors: Willcox
authors: Bergithe E V
authors: BE
authors: Oftedal
authors: Jone F
authors: JF
authors: Owe
authors: Nils Erik
authors: NE
authors: Gilhus
authors: Anthony
authors: A
authors: Meager
authors: Eystein S
authors: ES
authors: Husebye
authors: Anette S Bøe
authors: AS
authors: Wolff
mesh_terms: D000367:Age Factors; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D008297:Male; D009157:Myasthenia Gravis / Q000453:epidemiology / Q000276:immunology*; D016884:Polyendocrinopathies, Autoimmune / Q000453:epidemiology / Q000276:immunology*; D011869:Radioligand Assay; D016036:Seroepidemiologic Studies; D013945:Thymoma / Q000276:immunology; D013953:Thymus Neoplasms / Q000276:immunology
chemical_list: D001323:Autoantibodies; D007370:Interferon Type I