title: Self-interaction, nucleic acid binding, and nucleic acid chaperone activities are unexpectedly retained in the unique ORF1p of zebrafish LINE.
abstract: Long interspersed elements (LINEs) are mobile elements that comprise a large proportion of many eukaryotic genomes. Although some LINE-encoded open reading frame 1 proteins (ORF1ps) were suggested to be required for LINE mobilization through binding to their RNA, their general role is not known. The ZfL2-1 ORF1p, which belongs to the esterase-type ORF1p, is especially interesting because it has no known RNA-binding domain. Here we demonstrate that ZfL2-1 ORF1p has all the canonical activities associated with known ORF1ps, including self-interaction, nucleic acid binding, and nucleic acid chaperone activities. In particular, we showed that its chaperone activity is reversible, suggesting that the chaperone activities of many other ORF1ps are also reversible. From this discovery, we propose that LINE ORF1ps play a general role in LINE integration by forming a complex with LINE RNA and rearranging its conformation.
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa, Japan.
authors: Mitsuhiro
authors: M
authors: Nakamura
authors: Norihiro
authors: N
authors: Okada
authors: Masaki
authors: M
authors: Kajikawa
mesh_terms: D000818:Animals; D004950:Esterases / Q000235:genetics*; D020084:Long Interspersed Nucleotide Elements; D009696:Nucleic Acids / Q000378:metabolism*; D016366:Open Reading Frames; D011485:Protein Binding; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D009696:Nucleic Acids; D029961:Zebrafish Proteins; D004950:Esterases