title: NADH fluorescence as a photobiological metric in 5-aminolevlinic acid (ALA)-photodynamic therapy.
abstract: Photodynamic therapy (PDT) dosimetry is complex as many factors are involved and varied interdependently. Monitoring the biological consequence of PDT such as cell death is the most direct approach to assess treatment efficacy. In this study, we performed 5-aminolevlinic acid (ALA)-PDT in vitro to induce different cell death modes (i.e., slight cell cytotoxicity, apoptosis, and necrosis) by a fixed fluence rate of 10 mW/cm(2) and varied fluences (1, 2, and 6 J/cm(2)). Time course measurements of cell viability, caspase-3 activity, and DNA fragmentation were conducted to determine the mode of cell death. We demonstrated that NADH fluorescence lifetime together with NADH fluorescence intensity permit us to detect apoptosis and differentiate it from necrosis. This feature will be unique in the use of optimizing apoptosis-favored treatments such as metronomic PDT.
authors: Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong St., Sec. 2, Taipei 112, Taiwan.
authors: Guan-Chin
authors: GC
authors: Su
authors: Yau-Huei
authors: YH
authors: Wei
authors: Hsing-Wen
authors: HW
authors: Wang
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005453:Fluorescence; D016193:G1 Phase / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009243:NAD / Q000378:metabolism*; D009336:Necrosis; D018462:Photobiology; D010778:Photochemotherapy; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
chemical_list: D009243:NAD; D000622:Aminolevulinic Acid; D053148:Caspase 3