title: Vitamin C treatment promotes mesenchymal stem cell sheet formation and tissue regeneration by elevating telomerase activity.
abstract: Cell sheet engineering has been developed as an alternative approach to improve mesenchymal stem cell-mediated tissue regeneration. In this study, we found that vitamin C (Vc) was capable of inducing telomerase activity in periodontal ligament stem cells (PDLSCs), leading to the up-regulated expression of extracellular matrix type I collagen, fibronectin, and integrin β1, stem cell markers Oct4, Sox2, and Nanog as well as osteogenic markers RUNX2, ALP, OCN. Under Vc treatment, PDLSCs can form cell sheet structures because of increased cell matrix production. Interestingly, PDLSC sheets demonstrated a significant improvement in tissue regeneration compared with untreated control dissociated PDLSCs and offered an effective treatment for periodontal defects in a swine model. In addition, bone marrow mesenchymal stem cell sheets and umbilical cord mesenchymal stem cell sheets were also well constructed using this method. The development of Vc-mediated mesenchymal stem cell sheets may provide an easy and practical approach for cell-based tissue regeneration.
authors: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
authors: Fulan
authors: F
authors: Wei
authors: Cunye
authors: C
authors: Qu
authors: Tieli
authors: T
authors: Song
authors: Gang
authors: G
authors: Ding
authors: Zhipeng
authors: Z
authors: Fan
authors: Dayong
authors: D
authors: Liu
authors: Yi
authors: Y
authors: Liu
authors: Chunmei
authors: C
authors: Zhang
authors: Songtao
authors: S
authors: Shi
authors: Songlin
authors: S
authors: Wang
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013552:Swine; D013556:Swine, Miniature; D019098:Telomerase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014470:Umbilical Cord
chemical_list: D019098:Telomerase; D001205:Ascorbic Acid