title: An immunostimulatory polysaccharide (SCP-IIa) from the fruit of Schisandra chinensis (Turcz.) Baill.
abstract: A water-soluble polysaccharide named SCP-IIa was isolated from the water extract of the fruit of Schisandra chinensis (Turcz.) Baill by means of ethanol precipitation, deproteination, anion-exchange and gel-permeation chromatography. The molecular weight of SCP-IIa was ascertained via HPLC, and immuno-modulating effect was evaluated using the immunosuppressed model induced by cyclophosphamide. SCP-IIa was a homogeneous form of polysaccharide, with an average molecular weight of approximately 7700 Da. The detected parameters showed that SCP-IIa increased the thymus and spleen indices, as well as the pinocytic activity of the peritoneal macrophages in immunosuppressed mice. The splenocyte proliferation assay showed that SCP-IIa, in combination with Con A or LPS, positively affected splenocyte proliferation. Moreover, the polysaccharide promoted hemolysin formation. The results suggested that SCP-IIa was involved in immunomodulatory effects leading to the exploration for SCP-IIa as a potential immunostimulant.
authors: Department of Basic Medicine, Weifang Medical University, Weifang 261042, China.
authors: Yong
authors: Y
authors: Chen
authors: Jinbao
authors: J
authors: Tang
authors: Xiaoke
authors: X
authors: Wang
authors: Fengxiang
authors: F
authors: Sun
authors: Shujuan
authors: S
authors: Liang
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood; D005638:Fruit / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000097:blood; D007165:Immunosuppression Therapy; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D006460:Hemolysin Proteins; D011134:Polysaccharides