title: A simple yeast-based strategy to identify host cellular processes targeted by bacterial effector proteins.
abstract: Bacterial effector proteins, which are delivered into the host cell via the type III secretion system, play a key role in the pathogenicity of gram-negative bacteria by modulating various host cellular processes to the benefit of the pathogen. To identify cellular processes targeted by bacterial effectors, we developed a simple strategy that uses an array of yeast deletion strains fitted into a single 96-well plate. The array is unique in that it was optimized computationally such that despite the small number of deletion strains, it covers the majority of genes in the yeast synthetic lethal interaction network. The deletion strains in the array are screened for hypersensitivity to the expression of a bacterial effector of interest. The hypersensitive deletion strains are then analyzed for their synthetic lethal interactions to identify potential targets of the bacterial effector. We describe the identification, using this approach, of a cellular process targeted by the Xanthomonas campestris type III effector XopE2. Interestingly, we discover that XopE2 affects the yeast cell wall and the endoplasmic reticulum stress response. More generally, the use of a single 96-well plate makes the screening process accessible to any laboratory and facilitates the analysis of a large number of bacterial effectors in a short period of time. It therefore provides a promising platform for studying the functions and cellular targets of bacterial effectors and other virulence proteins.
authors: Department of Molecular Biology and Ecology of Plants, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Eran
authors: E
authors: Bosis
authors: Dor
authors: D
authors: Salomon
authors: Guido
authors: G
authors: Sessa
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D005804:Genes, Lethal / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D037521:Virulence Factors / Q000494:pharmacology*; D016959:Xanthomonas campestris / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors; D012967:Sodium Dodecyl Sulfate; D002110:Caffeine