title: Live-cell visualization of the trans-cellular mode of monocyte transmigration across the vascular endothelium, and its relationship with endothelial PECAM-1.
abstract: In response to atherogenic stimuli, blood monocytes transmigrate across the vascular endothelium not only through endothelial cell-cell junctions (para-cellular) but also through endothelial cells themselves (trans-cellular). The molecular mechanism of the latter is mostly unknown, because it rarely happens, especially in vitro. Although many reports have recognized trans-cellular migration from snapshot images of leukocytes halfway across the endothelium at non-junctional locations, it often produces a false-positive result, because some leukocytes that initiate trans-cellular migration withdraw and return to the apical endothelial surface. Thus, analyzing the entire process is essential. In this study, complete monocyte trans-cellular migration was successfully captured for live cells, with simultaneous visualization of endothelial PECAM-1. We suggest the possible existence of both PECAM-1-related migration at peri-junctional sites and PECAM-1-unrelated migration at sites remote from junctions. This is the first report to describe the entire process of monocyte trans-cellular migration for live cells and its relationship with endothelial PECAM-1.
authors: First Department of Physiology, Kawasaki Medical School, Kurashiki, Okayama, Japan. khashimo@med.kawasaki-m.ac.jp
authors: Ken
authors: K
authors: Hashimoto
authors: Noriyuki
authors: N
authors: Kataoka
authors: Emi
authors: E
authors: Nakamura
authors: Kimiko
authors: K
authors: Hagihara
authors: Takeaki
authors: T
authors: Okamoto
authors: Hiroaki
authors: H
authors: Kanouchi
authors: Satoshi
authors: S
authors: Mohri
authors: Katsuhiko
authors: K
authors: Tsujioka
authors: Fumihiko
authors: F
authors: Kajiya
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D007365:Intercellular Junctions; D009000:Monocytes / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000502:physiology*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1