title: Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation.
abstract: Marinesco bodies (MBs) are spherical eosinophilic intranuclear inclusions in pigmented neurons in the substantia nigra and locus ceruleus. Previous immunohistochemical studies have shown that MBs are positive for ubiquitin, p62 and SUMO-1, suggesting the involvement of ubiquitination and related proteins in the formation or disaggregation of MBs. However, the involvement is not thoroughly understood. Therefore, we immunohistochemically examined the midbrain from five control subjects ranged from 53 to 84 years old. MBs were positive for various proteins implicated in the ubiquitin-proteasome system (ubiquitin, p62, EDD1, NEDD8, NUB1, SUMO-1 and SUMO-2), aggresome formation (HDAC6) and autophagy (ubiquitin, p62, LC3, GABARAP and GATE-16). These findings suggest that proteins related to ubiquitination, proteasomal degradation and autophagy are involved in the formation or disaggregation of MBs.
authors: Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan. h001008@cc.hirosaki-u.ac.jp
authors: Saori
authors: S
authors: Odagiri
authors: Kunikazu
authors: K
authors: Tanji
authors: Fumiaki
authors: F
authors: Mori
authors: Akiyoshi
authors: A
authors: Kakita
authors: Hitoshi
authors: H
authors: Takahashi
authors: Tetsu
authors: T
authors: Kamitani
authors: Koichi
authors: K
authors: Wakabayashi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001343:Autophagy / Q000502:physiology*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D013378:Substantia Nigra / Q000473:pathology*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex