title: On-chip modulation of evanescent illumination and live-cell imaging with polymer waveguides.
abstract: Imaging of live cells was carried out using evanescent-wave excitation on a polymer waveguide chip. Integrated waveguide-based interferometric light modulators were fabricated in order to demonstrate on-chip control of excitation light, e.g., for time-lapse fluorescence microscopy. When combined with a sensitive high-resolution imaging system, the integrated waveguide-excitation platform provides an ideal method of near-surface studies of live cells, where photobleaching and/or phototoxicity effects are of critical concern.
authors: Department of Physics, Science Institute, University of Iceland, Dunhagi 3,IS-107 Reykjavik, Iceland.
authors: Björn
authors: B
authors: Agnarsson
authors: Asta B
authors: AB
authors: Jonsdottir
authors: Nina B
authors: NB
authors: Arnfinnsdottir
authors: Kristjan
authors: K
authors: Leosson
mesh_terms: D000818:Animals; D002470:Cell Survival; D022001:Focal Adhesions / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007368:Interferometry; D018374:LLC-PK1 Cells; D056656:Lab-On-A-Chip Devices; D008027:Light; D008853:Microscopy; D011108:Polymers / Q000737:chemistry*; D013552:Swine
chemical_list: D011108:Polymers