title: Fast and efficient MCR-based synthesis of clickable rhodamine tags for protein profiling.
abstract: Protein profiling probes are important tools for studying the composition of the proteome and as such have contributed greatly to the understanding of various complex biological processes in higher organisms. For this purpose the application of fluorescently labeled activity or affinity probes is highly desirable. Especially for in vivo detection of low abundant target proteins, otherwise difficult to analyse by standard blotting techniques, fluorescently labeled profiling probes are of high value. Here, a one-pot protocol for the synthesis of activated fluorescent labels (i.e. azide, alkynyl or NHS), based on the Ugi-4-component reaction (Ugi-4CR), is presented. As a result of the peptoidic structure formed, the fluorescent properties of the products are pH insensitive. Moreover, the applicability of these probes, as exemplified by the labeling of model protein BSA, will be discussed.
authors: Leibniz-Institute of Plant Biochemistry, Dept. of Bioorganic Chemistry, Weinberg 3, 06120, Halle, Germany.
authors: Sebastian
authors: S
authors: Brauch
authors: Michael
authors: M
authors: Henze
authors: Bianca
authors: B
authors: Osswald
authors: Kai
authors: K
authors: Naumann
authors: Ludger A
authors: LA
authors: Wessjohann
authors: Sander S
authors: SS
authors: van Berkel
authors: Bernhard
authors: B
authors: Westermann
mesh_terms: D000818:Animals; D002417:Cattle; D060326:Chemistry Techniques, Synthetic / Q000191:economics / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008958:Models, Molecular; D040901:Proteomics; D012235:Rhodamines / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012710:Serum Albumin, Bovine