title: Bio-isoprene production using exogenous MVA pathway and isoprene synthase in Escherichia coli.
abstract: In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiae MVA pathway and isoprene synthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVA pathway and ispS(Pa) gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVA pathway in E. coli has been provided.
authors: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
authors: Jianming
authors: J
authors: Yang
authors: Guang
authors: G
authors: Zhao
authors: Yuanzhang
authors: Y
authors: Sun
authors: Yanning
authors: Y
authors: Zheng
authors: Xinglin
authors: X
authors: Jiang
authors: Wei
authors: W
authors: Liu
authors: Mo
authors: M
authors: Xian
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D002070:Butadienes / Q000302:isolation & purification; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D024861:Genetic Enhancement / Q000379:methods*; D045782:Hemiterpenes / Q000096:biosynthesis* / Q000302:isolation & purification; D010420:Pentanes / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D011994:Recombinant Proteins; C005059:isoprene; D019883:Alkyl and Aryl Transferases; C093854:isoprene synthase