title: Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition.
abstract: Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.
authors: NanoBioLab, Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors: Miriam
authors: M
authors: Colombo
authors: Silvia
authors: S
authors: Sommaruga
authors: Serena
authors: S
authors: Mazzucchelli
authors: Laura
authors: L
authors: Polito
authors: Paolo
authors: P
authors: Verderio
authors: Patrizia
authors: P
authors: Galeffi
authors: Fabio
authors: F
authors: Corsi
authors: Paolo
authors: P
authors: Tortora
authors: Davide
authors: D
authors: Prosperi
mesh_terms: D000480:Alkynes / Q000737:chemistry; D045744:Cell Line, Tumor; D003500:Cyclization; D006801:Humans; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis* / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
chemical_list: D000480:Alkynes; D009589:Nitrogen Oxides; D018719:Erb-b2 Receptor Tyrosine Kinases; D057127:Single-Chain Antibodies; C015388:nitrones