title: Characterization of a weak allele of zebrafish cloche mutant.
abstract: Hematopoiesis is a complicated and dynamic process about which the molecular mechanisms remain poorly understood. Danio rerio (zebrafish) is an excellent vertebrate system for studying hematopoiesis and developmental mechanisms. In the previous study, we isolated and identified a cloche(172) (clo(172)) mutant, a novel allele compared to the original cloche (clo) mutant, through using complementation test and initial mapping. Here, according to whole mount in-situ hybridization, we report that the endothelial cells in clo(172) mutant embryos, although initially developed, failed to form the functional vascular system eventually. In addition, further characterization indicates that the clo(172) mutant exhibited weaker defects instead of completely lost in primitive erythroid cells and definitive hematopoietic cells compared with the clo(s5) mutant. In contrast, primitive myeloid cells were totally lost in clo(172) mutant. Furthermore, these reappeared definitive myeloid cells were demonstrated to initiate from the remaining hematopoietic stem cells (HSCs) in clo(172) mutant, confirmed by the dramatic decrease of lyc in clo(172)runx1(w84x) double mutant. Collectively, the clo(172) mutant is a weak allele compared to the clo(s5) mutant, therefore providing a model for studying the early development of hematopoietic and vascular system, as well as an opportunity to further understand the function of the cloche gene.
authors: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors: Ning
authors: N
authors: Ma
authors: Zhibin
authors: Z
authors: Huang
authors: Xiaohui
authors: X
authors: Chen
authors: Fei
authors: F
authors: He
authors: Kun
authors: K
authors: Wang
authors: Wei
authors: W
authors: Liu
authors: Linfeng
authors: L
authors: Zhao
authors: Xiangmin
authors: X
authors: Xu
authors: Wangjun
authors: W
authors: Liao
authors: Hua
authors: H
authors: Ruan
authors: Shenqiu
authors: S
authors: Luo
authors: Wenqing
authors: W
authors: Zhang
mesh_terms: D000483:Alleles; D000818:Animals; D001808:Blood Vessels / Q000196:embryology / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D004920:Erythropoiesis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D009154:Mutation / Q000235:genetics*; D038042:Myelopoiesis / Q000235:genetics; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins