title: [Pharmacokinetics of amphotericin B in the cerebrospinal fluid during continuous intrathecal administration for treatment of cryptococcal neoformans meningitis].
abstract: OBJECTIVE
  To explore the pharmacokinetics of amphotericin B (AMB) in the cerebrospinal fluid (CSF) during continuous intrathecal administration of AMB for treatment of cryptococcal neoformans meningitis (CNM).
  
  
  METHODS
  The concentration of AMB in the CSF was measured using reversed phase high performance liquid chromatography (RP-HPLC) in 3 patients receiving continuous intrathecal infusion of AMB for CNM.
  
  
  RESULTS
  AMB concentrations in the CSF of the 3 patients exceeded the minimal inhibitory concentration (MIC) of AMB against Cryptococcus neoformans. The concentration-time curve showed that AMB concentration in the CSF underwent obvious variations on the first day of intrathecal infusion and after additional AMB doses, but maintained a stable level (0.61-1.21 µg/ml) on the next day.
  
  
  CONCLUSIONS
  [corrected] Continuous intrathecal administration of AMB can enhance the drug concentration in the CSF and maintain a stable and effective drug level for treatment of CNM.
authors: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Min
authors: M
authors: Fang
authors: Tian-ming
authors: TM
authors: Lv
authors: Yuan
authors: Y
authors: Yan
authors: Shi-ting
authors: ST
authors: Liu
authors: Wen-hui
authors: WH
authors: Gao
authors: Xiao-jia
authors: XJ
authors: Liu
authors: Jia
authors: J
authors: Yin
authors: Li
authors: L
authors: Wang
authors: Yuan-yuan
authors: YY
authors: Wang
mesh_terms: D000293:Adolescent; D000666:Amphotericin B / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D000935:Antifungal Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D002555:Cerebrospinal Fluid / Q000378:metabolism; D003455:Cryptococcus neoformans / Q000302:isolation & purification; D005260:Female; D006801:Humans; D060186:Infusions, Spinal / Q000379:methods*; D008297:Male; D016919:Meningitis, Cryptococcal / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D000666:Amphotericin B