title: Rhythmic interaction between Period1 mRNA and hnRNP Q leads to circadian time-dependent translation.
abstract: The mouse PERIOD1 (mPER1) protein, along with other clock proteins, plays a crucial role in the maintenance of circadian rhythms. mPER1 also provides an important link between the circadian system and the cell cycle system. Here we show that the circadian expression of mPER1 is regulated by rhythmic translational control of mPer1 mRNA together with transcriptional modulation. This time-dependent translation was controlled by an internal ribosomal entry site (IRES) element in the 5' untranslated region (5'-UTR) of mPer1 mRNA along with the trans-acting factor mouse heterogeneous nuclear ribonucleoprotein Q (mhnRNP Q). Knockdown of mhnRNP Q caused a decrease in mPER1 levels and a slight delay in mPER1 expression without changing mRNA levels. The rate of IRES-mediated translation exhibits phase-dependent characteristics through rhythmic interactions between mPer1 mRNA and mhnRNP Q. Here, we demonstrate 5'-UTR-mediated rhythmic mPer1 translation and provide evidence for posttranscriptional regulation of the circadian rhythmicity of core clock genes.
authors: Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Gyeongbuk, Republic of Korea.
authors: Kyung-Ha
authors: KH
authors: Lee
authors: Kyung-Chul
authors: KC
authors: Woo
authors: Do-Yeon
authors: DY
authors: Kim
authors: Tae-Don
authors: TD
authors: Kim
authors: Jaecheon
authors: J
authors: Shin
authors: Sung Mi
authors: SM
authors: Park
authors: Sung Key
authors: SK
authors: Jang
authors: Kyong-Tai
authors: KT
authors: Kim
mesh_terms: D000818:Animals; D057906:Circadian Clocks / Q000235:genetics; D002940:Circadian Rhythm; D005786:Gene Expression Regulation / Q000235:genetics; D055785:Gene Knockdown Techniques; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; C486049:Syncrip protein, mouse