title: [Honokiol-induced apoptosis of human non-Hodgkin lymphoma Raji cells and its  possible  mechanism].
abstract: OBJECTIVE
  To investigate the apoptosis-inducing effect of honokiol on human non-Hodgkin lymphoma Raji cells and the possible mechanism.
  
  
  METHODS
  Raji cells were treated with different concentrations of honokiol, and the proliferation of the cells was detected using MTT assay. Flow cytometry was employed to analyze the cell cycle changes and apoptosis of honokiol-treated cells. Caspase 8 activity in the cells was measured by caspase 8 kit, and RT-PCR was used to detect the expression of apoptosis-related genes Bcl-2, Bad, and Bax.
  
  
  RESULTS
  Honokiol significantly inhibited the growth of Raji cells in a time- and dose-dependent manner, with IC(50) concentration of 17.53, 12.61, and 7.4  µg/ml at 12, 24, 48 h, respectively. Flow cytometry revealed cell cycle arrest at G0/G1 phase following honokiol treatment. The apoptosis rates of Raji cells treated with 7.5 and 15 µg/ml honokiol were significantly higher than that of the control cells [(18.24∓2.53)%, (28.44∓2.48)% vs (4.84∓1.15)%, P<0.01]. Caspase 8 activity in Raji cells was significantly enhanced by honokiol (P<0.05). The mRNA expression of the apoptosis-promoting gene Bad was significantly increased following honokiol treatment (P<0.01), while the expressions of Bcl-2 and Bax remained unchanged.
  
  
  CONCLUSIONS
  Honokiol can induce apoptosis in Raji cells possibly in relation to enhancement of caspase 8 activity and Bad gene expression.
authors: Department of Hematology, Guangdong Second Provincial People's Hospital, Guangzhou 510317, China. cwei61@126.com
authors: Wei
authors: W
authors: Chen
authors: Guan-wen
authors: GW
authors: Lin
authors: Qing
authors: Q
authors: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002051:Burkitt Lymphoma / Q000473:pathology; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D051025:bcl-Associated Death Protein / Q000235:genetics / Q000378:metabolism; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D053181:Caspase 8; D017705:Lignans; D051025:bcl-Associated Death Protein; C494819:BAD protein, human; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C505530:CASP8 protein, human