title: [Effects of damage-regulated autophagy modulator on the radiosensitivity of SGC7901 cell xenografts in nude mice].
abstract: OBJECTIVE
  To investigate the effects of damage-regulated autophagy modulator (DRAM) on radiosensitivity and the related mechanisms of implanted tumors of SGC7901 human gastric carcinoma cells in nude mice.
  
  
  METHODS
  Nude mice were randomly divided into model control group, radiotherapy group, and DRAM treatment group and radiotherapy combined with DRAM treatment group. When volume of transplantation tumor were 1.0 cm(3), radiotherapy, DRAM treatment was given. On days 3, 6 and 9 after treatment, the inhibition rate of tumor growth, pathological changes in tumor specimens, expression levels of P53, proliferating cell nuclear antigen(PCNA), C-myc, Fas-L, as well as apoptosis indexes in tumor samples were observed.
  
  
  RESULTS
  Inhibition rates of tumor in DRAM combined with radiotherapy were 9.3%, 14.1%, 16.7% on day 3, 6 and 9, respectively, all significantly higher than those in the radiotherapy group(5.0%, 8.8%, 6.5%, P<0.05). The expressions of PCNA and C-myc protein were down-regulated, while the expressions of P53 and Fas-L were upregulated.
  
  
  CONCLUSIONS
  Damage-regulated autophagy modulator gene may promote cell apoptosis and inhibit cell growth to enhance the radiosensitivity of transplanted gastric tumor in vivo in nude mice.
authors: Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China. xingcg@126.com.
authors: Kui
authors: K
authors: Zhao
authors: Wei
authors: W
authors: Gong
authors: Bao-song
authors: BS
authors: Zhu
authors: Yong-you
authors: YY
authors: Wu
authors: Xiao-dong
authors: XD
authors: Yang
authors: Yong
authors: Y
authors: Wu
authors: Chun-gen
authors: CG
authors: Xing
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured