title: Down-regulation of P-glycoprotein expression contributes to an increase             in Danshensu accumulation in the cerebral ischemia/reperfusion brain.
abstract: P-glycoprotein (P-gp) plays a key role in the distribution and elimination             of many clinically important therapeutic substances. P-gp inhibition is capable             of increasing the concentration of various drugs in the brain. Danshensu is a             main hydrophilic constituent of Salvia miltiorrhiza Bunge which is used traditionally             to treat cerebrovascular diseases. In the present study, the effects of cerebral             ischemia/reperfusion on the transport of Danshensu in the brain were observed             by assaying the concentration at 15, 30 and 60 min after cerebral ischemia/reperfusion             in the brain of rats. Furthermore, the expression of P-gp in the brain was also             measured. Results showed that Danshensu concentrations in the ischemic hemisphere             significantly increased compared to those in the non-ischemic hemisphere and the             brain of the sham group. P-gp expression was inhibited by cerebral ischemia/reperfusion             to some extent, and down-regulated much more by Danshensu treatment. The findings             indicate that the down-regulation of P-gp expression induced by both cerebral             ischemia/reperfusion and Danshensu treatment contributes to the increase of Danshensu             accumulation in the brain.
authors: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, PR China.
authors: Yating
authors: Y
authors: Chong
authors: Tian
authors: T
authors: Wang
authors: Wenyan
authors: W
authors: Wang
authors: Leiming
authors: L
authors: Zhang
authors: Chong
authors: C
authors: Li
authors: Pengfei
authors: P
authors: Yu
authors: Hongsheng
authors: H
authors: Wang
authors: Fenghua
authors: F
authors: Fu
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism / Q000627:therapeutic use; D007773:Lactates / Q000032:analysis / Q000378:metabolism* / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid