title: Coexistence of blaOXA-48 and aac(6')-Ib-cr genes in Klebsiella pneumoniae isolates from Istanbul, Turkey.
abstract: This study evaluated the presence of carbapenem hydrolysing β-lactamase genes and plasmid-mediated quinolone resistance (PMQR) determinants in 22 Klebsiella pneumoniae isolates collected from the Istanbul Medical Faculty, Turkey, which reduced the susceptibility or resistance  to carbapenem. The VITEK(®) 2 system and E-tests were used to determine the minimum inhibitory concentrations needed to inhibit bacterial growth. Genes were screened by polymerase chain reaction, and gene transferability was evaluated by transconjugation. Strain clonality was  investigated by pulsed-field gel electrophoresis (PFGE). All strains were OXA-48 β-lactamase producers and three (13.6%) were also positive for the aac(6')-Ib-cr gene. Most of the strains harboured other β-lactamase (bla) genes such as bla(TEM), bla(SHV),  bla(CTX-M) and bla(VEB-1). The transconjugants mostly harboured bla(OXA-48) and other β-lactamases separately. PFGE revealed eight pulsotypes among the isolates. The coexistence of bla(OXA-48) and PMQR in K. pneumoniae  isolates may present a significant threat to health, especially in the nosocomial setting.
authors: Department of Microbiology and Clinical Microbiology, Istanbul Medical Faculty, Istanbul University, Millet Street, Capa, Fatih, Istanbul 34093, Turkey. hasannazik01@gmail.com
authors: H
authors: H
authors: Nazik
authors: B
authors: B
authors: Öngen
authors: B
authors: B
authors: Mete
authors: S
authors: S
authors: Aydin
authors: M
authors: M
authors: Yemişen
authors: F M
authors: FM
authors: Keleşoğlu
authors: Y
authors: Y
authors: Ergul
authors: F
authors: F
authors: Tabak
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015780:Carbapenems / Q000494:pharmacology* / Q000627:therapeutic use; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D019450:Genes, MDR; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology / Q000401:mortality; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D015242:Ofloxacin / Q000494:pharmacology*; D010957:Plasmids; D014421:Turkey; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D015242:Ofloxacin; D001618:beta-Lactamases