title: Unravelling global genome organization by 3C-seq.
abstract: Eukaryotic genomes exist in the cell nucleus as an elaborate three-dimensional structure which reflects various nuclear processes such as transcription, DNA replication and repair. Next-generation sequencing (NGS) combined with chromosome conformation capture (3C), referred to as 3C-seq in this article, has recently been applied to the yeast and human genomes, revealing genome-wide views of functional associations among genes and their regulatory elements. Here, we compare the latest genomic approaches such as 3C-seq and ChIA-PET, and provide a condensed overview of how eukaryotic genomes are functionally organized in the nucleus.
authors: The Wistar Institute, Philadelphia, PA, USA.
authors: Hideki
authors: H
authors: Tanizawa
authors: Ken-ichi
authors: K
authors: Noma
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods; D045584:Chromosome Positioning; D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA / Q000379:methods*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D004271:DNA, Fungal