title: Rubella virus-like replicon particles: analysis of encapsidation determinants and non-structural roles of capsid protein in early post-entry replication.
abstract: Rubella virus (RUBV) contains a plus-strand RNA genome with two ORFs, one encoding the non-structural replicase proteins (NS-ORF) and the second encoding the virion structural proteins (SP-ORF). This study describes development and use of a trans-encapsidation system for the assembly of infectious RUBV-like replicon particles (VRPs) containing RUBV replicons (self replicating genomes with the SP-ORF replaced with a reporter gene). First, this system was used to map signals within the RUBV genome that mediate packaging of viral RNA. Mutations within a proposed packaging signal did not significantly affect relative packaging efficiency. The insertion of various fragments derived from the RUBV genome into Sindbis virus replicons revealed that there are several regions within the RUBV genome capable of enhancing encapsidation of heterologous replicon RNAs. Secondly, the trans-encapsidation system was used to analyse the effect of alterations within the capsid protein (CP) on release of VRPs and subsequent initiation of replication in newly infected cells. Deletion of the N-terminal eight amino acids of the CP reduced VRP titre significantly, which could be partially complemented by native CP provided in trans, indicating that this mutation affected an entry or post-entry event in the replication cycle. To test this hypothesis, the trans-encapsidation system was used to demonstrate the rescue of a lethal deletion within P150, one of the virus replicase proteins, by CP contained within the virus particle. This novel finding substantiated the functional role of CP in early post-entry replication.
authors: Institute of Virology, University of Leipzig, Leipzig, Germany.
authors: Claudia
authors: C
authors: Claus
authors: Department of Biology, Georgia State University, Atlanta, GA, USA.
authors: Wen-Pin
authors: WP
authors: Tzeng
authors: Institute of Virology, University of Leipzig, Leipzig, Germany.
authors: U G
authors: UG
authors: Liebert
authors: Department of Biology, Georgia State University, Atlanta, GA, USA.
authors: Teryl K
authors: TK
authors: Frey
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D005816:Genetic Complementation Test; D012367:RNA, Viral / Q000235:genetics; D012093:Replicon; D012412:Rubella virus / Q000502:physiology*; D017384:Sequence Deletion; D012845:Sindbis Virus; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral