title: Vaccination with parenteral toxoid B protects hamsters against lethal challenge with toxin A-negative, toxin B-positive clostridium difficile but does not prevent colonization.
abstract: Toxin A has historically been regarded as the primary virulence determinant in Clostridium difficile infection, but naturally occurring toxin A-negative, toxin B-positive (A-/B+) C. difficile strains are known to be virulent. To determine the role of toxin B in these strains, we immunized hamsters with a toxoid prepared from purified toxin B to determine whether they would be protected from lethal challenge with an A-/B+ strain of C. difficile.
authors: Research Service, Hines VA Hospital, Hines, IL 60141, USA.
authors: Farida
authors: F
authors: Siddiqui
authors: Jennifer R
authors: JR
authors: O'Connor
authors: Kristin
authors: K
authors: Nagaro
authors: Adam
authors: A
authors: Cheknis
authors: Susan P
authors: SP
authors: Sambol
authors: Gayatri
authors: G
authors: Vedantam
authors: Dale N
authors: DN
authors: Gerding
authors: Stuart
authors: S
authors: Johnson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D016360:Clostridioides difficile / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D003015:Clostridium Infections / Q000276:immunology* / Q000382:microbiology; D006224:Cricetinae; D004768:Enterotoxins / Q000378:metabolism*; D014121:Toxoids / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D014121:Toxoids; D037521:Virulence Factors; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile