title: Quantitative characterization of articular cartilage using Mueller matrix imaging and multiphoton microscopy.
abstract: The collagen meshwork in articular cartilage of chicken knee is characterized using Mueller matrix imaging and multiphoton microscopy. Direction and degree of dispersion of the collagen fibers in the superficial layer are found using a Fourier transform image-analysis technique of the second-harmonic generated image. Mueller matrix images are used to acquire structural data from the intermediate layer of articular cartilage where the collagen fibers are too small to be resolved by optical microscopy, providing a powerful multimodal measurement technique. Furthermore, we show that Mueller matrix imaging provides more information about the tissue compared to standard polarization microscopy. The combination of these techniques can find use in improved diagnosis of diseases in articular cartilage, improved histopathology, and additional information for accurate biomechanical modeling of cartilage.
authors: Norwegian University of Science and Technology, Department of Physics, Faculty of Natural Sciences and Technology, Institutt for Fysikk, Trondheim, Norway.
authors: Pål Gunnar
authors: PG
authors: Ellingsen
authors: Magnus Borstad
authors: MB
authors: Lilledahl
authors: Lars Martin Sandvik
authors: LM
authors: Aas
authors: Catharina de Lange
authors: Cde L
authors: Davies
authors: Morten
authors: M
authors: Kildemo
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology* / Q000737:chemistry*; D002645:Chickens; D003952:Diagnostic Imaging / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton; D008859:Microscopy, Polarization / Q000379:methods*; D012815:Signal Processing, Computer-Assisted