title: Multifunctional nanoplatforms for fluorescence imaging and photodynamic therapy developed by post-loading photosensitizer and fluorophore to polyacrylamide nanoparticles.
abstract: We report a novel post-loading approach for constructing a multifunctional biodegradable polyacrylamide (PAA) nanoplatform for tumor-imaging (fluorescence) and photodynamic therapy (PDT). This approach provides an opportunity to post-load the imaging and therapeutic agents at desired concentrations. Among the PAA nanoparticles, a formulation containing the photosensitizer, HPPH [3-(1'-hexyloxyethyl)pyropheophorbide-a], and the cyanine dye in a ratio of 2:1 minimized the undesirable quenching of the HPPH electronic excitation energy because of energy migration within the nanoparticles and/or Förster (fluorescence) resonance energy transfer (FRET) between HPPH and cyanine dye. An excellent tumor-imaging (NIR fluorescence) and phototherapeutic efficacy of the nanoconstruct formulation is demonstrated. Under similar treatment parameters the HPPH in 1% Tween 80/5% aqueous dextrose formulation was less effective than the nanoconstruct containing HPPH and cyanine dye in a ratio of 2 to 1. This is the first example showing the use of the post-loading approach in developing a nanoconstructs for tumor-imaging and therapy.
authors: PDT Center, Buffalo, New York 14263, USA.
authors: Anurag
authors: A
authors: Gupta
authors: Shouyan
authors: S
authors: Wang
authors: Paula
authors: P
authors: Pera
authors: K V R
authors: KV
authors: Rao
authors: Nayan
authors: N
authors: Patel
authors: Tymish Y
authors: TY
authors: Ohulchanskyy
authors: Joseph
authors: J
authors: Missert
authors: Janet
authors: J
authors: Morgan
authors: Yong-Eun
authors: YE
authors: Koo-Lee
authors: Raoul
authors: R
authors: Kopelman
authors: Ravindra K
authors: RK
authors: Pandey
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D003287:Contrast Media / Q000627:therapeutic use; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053769:Nanocapsules / Q000737:chemistry / Q000627:therapeutic use*; D061848:Optical Imaging / Q000379:methods; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D016896:Treatment Outcome; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003287:Contrast Media; D005456:Fluorescent Dyes; D053769:Nanocapsules; D017319:Photosensitizing Agents; D000099166:Polyacrylamides