title: A microfabricated micropillar liquid chromatographic chip monolithically integrated with an electrospray ionization tip.
abstract: We present the first monolithically integrated silicon/glass liquid chromatography-electrospray ionization microchip for mass spectrometry. The microchip is fabricated by bonding a silicon wafer, which has deep reactive ion etched micropillar-filled channels, together with a glass lid. Both the silicon channel and the glass lid have a through-wafer etched sharp tip that produces a stable electrospray. The microchip is also compatible with laser induced fluorescence (LIF) detection, due to the glass lid. Separation of drugs in less than 5 minutes using either SiO(2) (normal phase) or C(18) coated (reversed-phase) pillars with good sensitivity was demonstrated with mass spectrometric detection as well as separation of fluorescent compounds with LIF detection.
authors: University of Helsinki, Division of Pharmaceutical Chemistry, FI-00014, Helsinki, Finland.
authors: Lauri
authors: L
authors: Sainiemi
authors: Teemu
authors: T
authors: Nissilä
authors: Risto
authors: R
authors: Kostiainen
authors: Sami
authors: S
authors: Franssila
authors: Raimo A
authors: RA
authors: Ketola
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005898:Glass / Q000737:chemistry; D055616:Microtechnology; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations; D012822:Silicon Dioxide