title: Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects.
abstract: BACKGROUND
  Platelet-rich fibrin (PRF) by Choukroun's technique is derived from an autogenous preparation of concentrated platelets. Little is known about the effects of PRF on periodontal ligament fibroblasts (PDLFs) and the application of PRF for periodontal regeneration.
  
  
  METHODS
  PDLFs were derived from healthy individuals undergoing extraction for orthodontic reasons. Blood collection was carried out from healthy volunteers. PRF was obtained from a table centrifuge centrifuged at 3000 rpm for 12 minutes. The effects of PRF on PDLFs were determined by measuring the expression of phosphorylated extracellular signal-regulated protein kinase (p-ERK), osteoprotegerin (OPG) and alkaline phosphatase (ALP) activity. Moreover, we retrospectively examined the feasibility and safety of reconstructing the periodontal infrabony defects with PRF in six patients.
  
  
  RESULTS
  PRF was found to increase ERK phosphorylation and OPG in PDLFs in a time-dependent manner (p < 0.05). ALP activity was also significantly upregulated by PRF (p < 0.05). Application of PRF in infrabony defects exhibited pocket reduction and clinical attachment gain after six months. Periapical radiography revealed radiographic defect filled in grafted teeth.
  
  
  CONCLUSIONS
  The enhancement of p-ERK, OPG and ALP expression by PRF may provide benefits for periodontal regeneration. Clinical and radiologic analysis showed that the use of PRF is an effective modality for periodontal infrabony defects.
authors: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan. cyc@csmu.edu.tw
authors: Y-C
authors: YC
authors: Chang
authors: J-H
authors: JH
authors: Zhao
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000601:surgery*; D000704:Analysis of Variance; D001792:Blood Platelets; D001861:Bone Regeneration / Q000187:drug effects; D002478:Cells, Cultured; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005337:Fibrin / Q000494:pharmacology / Q000627:therapeutic use*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D053244:Osteoprotegerin / Q000096:biosynthesis; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D012038:Regeneration / Q000187:drug effects; D012189:Retrospective Studies
chemical_list: D008567:Membranes, Artificial; D053244:Osteoprotegerin; D005337:Fibrin; D048049:Extracellular Signal-Regulated MAP Kinases; D000469:Alkaline Phosphatase