title: P-Rex1 is required for efficient melanoblast migration and melanoma metastasis.
abstract: Metastases are the major cause of death from melanoma, a skin cancer that has the                 fastest rising incidence of any malignancy in the Western world. Molecular pathways                 that drive melanoblast migration in development are believed to underpin the                 movement and ultimately the metastasis of melanoma. Here we show that mice lacking                 P-Rex1, a Rac-specific Rho GTPase guanine nucleotide exchange factor, have a                 melanoblast migration defect during development evidenced by a white belly.                 Moreover, these P-Rex1(-/-) mice are                 resistant to metastasis when crossed to a murine model of melanoma. Mechanistically,                 this is associated with P-Rex1 driving invasion in a Rac-dependent manner. P-Rex1 is                 elevated in the majority of human melanoma cell lines and tumour tissue. We conclude                 that P-Rex1 has an important role in melanoblast migration and cancer progression to                 metastasis in mice and humans.
authors: The Beatson Institute for Cancer Research, Glasgow G61 1BD, UK.
authors: Colin R
authors: CR
authors: Lindsay
authors: Samuel
authors: S
authors: Lawn
authors: Andrew D
authors: AD
authors: Campbell
authors: William J
authors: WJ
authors: Faller
authors: Florian
authors: F
authors: Rambow
authors: Richard L
authors: RL
authors: Mort
authors: Paul
authors: P
authors: Timpson
authors: Ang
authors: A
authors: Li
authors: Patrizia
authors: P
authors: Cammareri
authors: Rachel A
authors: RA
authors: Ridgway
authors: Jennifer P
authors: JP
authors: Morton
authors: Brendan
authors: B
authors: Doyle
authors: Shauna
authors: S
authors: Hegarty
authors: Mairin
authors: M
authors: Rafferty
authors: Ian G
authors: IG
authors: Murphy
authors: Enda W
authors: EW
authors: McDermott
authors: Kieran
authors: K
authors: Sheahan
authors: Katherine
authors: K
authors: Pedone
authors: Alexander J
authors: AJ
authors: Finn
authors: Pamela A
authors: PA
authors: Groben
authors: Nancy E
authors: NE
authors: Thomas
authors: Honglin
authors: H
authors: Hao
authors: Craig
authors: C
authors: Carson
authors: Jim C
authors: JC
authors: Norman
authors: Laura M
authors: LM
authors: Machesky
authors: William M
authors: WM
authors: Gallagher
authors: Ian J
authors: IJ
authors: Jackson
authors: Leon
authors: L
authors: Van Kempen
authors: Friedrich
authors: F
authors: Beermann
authors: Channing
authors: C
authors: Der
authors: Lionel
authors: L
authors: Larue
authors: Heidi C
authors: HC
authors: Welch
authors: Brad W
authors: BW
authors: Ozanne
authors: Owen J
authors: OJ
authors: Sansom
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009362:Neoplasm Metastasis / Q000235:genetics / Q000503:physiopathology*; D046888:Tissue Array Analysis
chemical_list: D020662:Guanine Nucleotide Exchange Factors; C511197:PREX1 protein, mouse