title: Rapid detection of blaIMP-6 by amplification refractory mutation system.
abstract: Klebsiella pneumoniae resistant to almost all ß-lactams except imipenem designated as ISMRK (imipenem-susceptible meropenem-resistant Klebsiella) is emerging in Japan. All ISMRK carries bla(IMP-6) which differs from bla(IMP-1) by only a single nucleotide at position 640. We devised a rapid detection system of bla(IMP-6) by using ARMS PCR.
authors: Project Research Center for Nosocomial Infectious Disease, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8551, Japan.
authors: Shizuo
authors: S
authors: Kayama
authors: Norifumi
authors: N
authors: Shigemoto
authors: Ryuichi
authors: R
authors: Kuwahara
authors: Makoto
authors: M
authors: Onodera
authors: Michiya
authors: M
authors: Yokozaki
authors: Hiroki
authors: H
authors: Ohge
authors: Fuminori
authors: F
authors: Kato
authors: Jyunzo
authors: J
authors: Hisatsune
authors: Motoyuki
authors: M
authors: Sugai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007564:Japan; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015378:Imipenem; D001618:beta-Lactamases