title: Rapid determination of perv copy number from porcine genomic DNA by real-time polymerase chain reaction.
abstract: Here, we report the quantification of porcine endogenous retrovirus (PERV) copy numbers using real time PCR. After generating standard curves using plasmid DNA, copy numbers were determined for PERV pol and for a housekeeping gene, porcine estrogen receptor2 (ER2) with the same amount of genomic DNA. Using this method, we examined 6 pig breeds in Korea including two breeds of miniature pig, one domestic pig from Jeju, and imported pig breeds, Duroc, Landrace, and Yorkshire. All breeds showed PERV copy numbers ranging from 9 to 50. This method will be useful for monitoring of PERVs in a porcine xenograft.
authors: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors: Donghee
authors: D
authors: Lee
authors: Jungeun
authors: J
authors: Lee
authors: Jong-Kwang
authors: JK
authors: Yoon
authors: Na Young
authors: NY
authors: Kim
authors: Gye-Woong
authors: GW
authors: Kim
authors: Chankyu
authors: C
authors: Park
authors: Yu-Kyoung
authors: YK
authors: Oh
authors: Young Bong
authors: YB
authors: Kim
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification*; D018628:Gene Dosage; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013552:Swine; D013556:Swine, Miniature
chemical_list: D004279:DNA, Viral; D004247:DNA