title: Identification of peptide motif that binds to the surface of zirconia.
abstract: A zirconia-binding peptide motif was identified using a peptide phage display system. Yttria stabilized zirconia beads and discs were used as the target. Quartz crystal microbalance was used to monitor the binding of phages to zirconia. Starting from a library of phages displaying random sequences of 12-mer peptides, we repeated cycles of biopanning against zirconia beads. After four cycles of biopanning, we isolated a phage clone Φ#17. DNA sequencing of the corresponding portion of Φ#17 unexpectedly revealed that it displayed a 58-mer peptide (amino acid sequence: WMPSDVDINDPQGGGSRPNLHQPKPAAEAASKKKSENRKVPFYSHSWY-SSMSEDKRGW). We found that Φ#17 had a 300-fold, significantly higher binding affinity for zirconia discs than phages displaying no peptide. In quartz crystal microbalance assay, a rapid increase in energy dissipation was observed from Φ#17 but not from the control phages, indicating that Φ#17 binds to the surface of zirconia via its displayed peptide. We successfully identified a peptide motif that binds zirconia.
authors: Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College.
authors: Kazuhiko
authors: K
authors: Hashimoto
authors: Masao
authors: M
authors: Yoshinari
authors: Kenichi
authors: K
authors: Matsuzaka
authors: Kiyotaka
authors: K
authors: Shiba
authors: Takashi
authors: T
authors: Inoue
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism*; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D015019:Yttrium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015921:Dental Implants; D003764:Dental Materials; D019151:Peptide Library; D010455:Peptides; D015019:Yttrium; D015040:Zirconium; C028541:zirconium oxide; C091417:yttria