title: A method for obtaining Schwann cell cultures from adult rabbit nerve based on "in vitro" pre-degeneration and neuregulin treatment.
abstract: Schwann cells (SCs) are basic elements for cell therapy and tissue engineering in the central and peripheral nervous system. Therefore, the development of a reliable method to obtain SC cultures is required. For possible therapeutic applications the cultures need to produce a sufficiently large number of SCs with a high level of purity in a relatively short period of time. To increase SC yield and purity we pre-degenerated pieces of 1-2 mm of adult rabbit sciatic nerves by incubating them for seven days in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin/streptomycin and NRG1-β1. Following pre-degeneration the nerve pieces were dissociated and then cultured for 6 or 15 days in the same culture medium. After 6 days of culture we obtained around 9.5x10³ cells/mg with approximately 94% SCs (S-100 positive) purity. After 15 days of culture the yield was about 80x10³ cells/mg and the purity was approximately 75%. Pre-degeneration and subsequent culture of small pieces of adult nerve with NRG1-β1 supplemented medium increased the number of SCs and restricted the overgrowth of fibroblast-like cells.
authors: Department of Cell Biology, Histology and Pharmacology, Institute of Neurosciences of Castile and Leon, (INCYL), University of Valladolid, Spain.
authors: I
authors: I
authors: de la Fuente
authors: I
authors: I
authors: Alcalde
authors: O L
authors: OL
authors: Gamboa
authors: M
authors: M
authors: Garrosa
authors: M J
authors: MJ
authors: Gayoso
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D008297:Male; D009410:Nerve Degeneration / Q000473:pathology*; D020890:Neuregulin-1 / Q000494:pharmacology*; D011817:Rabbits; D012583:Schwann Cells / Q000187:drug effects* / Q000473:pathology; D012584:Sciatic Nerve / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D020890:Neuregulin-1; C117467:neuregulin beta