title: Analysis of the biological activity of a novel 24-membered macrolide JBIR-19 in Saccharomyces cerevisiae by the morphological imaging program CalMorph.
abstract: To investigate the biological activity of a novel 24-membered macrolide compound, JBIR-19, isolated from the culture broth of the entomopathogenic fungus Metarhizium sp. fE61, morphological changes in yeast cells were examined using the automated image-processing program CalMorph. Principal components analysis was used to elucidate dynamic changes in the phenotypes, revealing two independent effects of JBIR-19 in yeast cells: bud elongation and increased size of the actin region. Using a fitness assay, we identified the genes required for robust growth in the presence of JBIR-19. Among these were CCW12, YLR111W, and DHH1, which are also involved in abnormal bud morphology. Based on these results and others, we predict intracellular targets of JBIR-19 and its functional interactions.
authors: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.
authors: Shinsuke
authors: S
authors: Ohnuki
authors: Tomohide
authors: T
authors: Kobayashi
authors: Hayato
authors: H
authors: Ogawa
authors: Ikuko
authors: I
authors: Kozone
authors: Jun-Ya
authors: JY
authors: Ueda
authors: Motoki
authors: M
authors: Takagi
authors: Kazuo
authors: K
authors: Shin-Ya
authors: Dai
authors: D
authors: Hirata
authors: Satoru
authors: S
authors: Nogami
authors: Yoshikazu
authors: Y
authors: Ohya
mesh_terms: D000199:Actins / Q000378:metabolism; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018942:Macrolides / Q000378:metabolism / Q000494:pharmacology*; D052981:Metarhizium / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010641:Phenotype; D025341:Principal Component Analysis; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012984:Software
chemical_list: D000199:Actins; D000935:Antifungal Agents; C544244:JBIR-19; D018942:Macrolides; D029701:Saccharomyces cerevisiae Proteins