title: Characterization of the stress associated microRNAs in Glycine max by deep sequencing.
abstract: BACKGROUND
  Plants involved in highly complex and well-coordinated systems have evolved a considerable degree of developmental plasticity, thus minimizing the damage caused by stress. MicroRNAs (miRNAs) have recently emerged as key regulators in gene regulation, developmental processes and stress tolerance in plants.
  
  
  RESULTS
  In this study, soybean miRNAs associated with stress responses (drought, salinity, and alkalinity) have been identified and analyzed in combination with deep sequencing technology and in-depth bioinformatics analysis. One hundred and thirty three conserved miRNAs representing 95 miRNA families were expressed in soybeans under three treatments. In addition, 71, 50, and 45 miRNAs are either uniquely or differently expressed under drought, salinity, and alkalinity, respectively, suggesting that many miRNAs are inducible and are differentially expressed in response to certain stress.
  
  
  CONCLUSIONS
  Our study has important implications for further identification of gene regulation under abiotic stresses and significantly contributes a complete profile of miRNAs in Glycine max.
authors: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin 130118, China. iamwujy@yahoo.com.cn
authors: Haiyan
authors: H
authors: Li
authors: Yuanyuan
authors: Y
authors: Dong
authors: Hailong
authors: H
authors: Yin
authors: Nan
authors: N
authors: Wang
authors: Jing
authors: J
authors: Yang
authors: Xiuming
authors: X
authors: Liu
authors: Yanfang
authors: Y
authors: Wang
authors: Jinyu
authors: J
authors: Wu
authors: Xiaokun
authors: X
authors: Li
mesh_terms: D055864:Droughts; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006863:Hydrogen-Ion Concentration; D035683:MicroRNAs / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D054712:Salinity; D017423:Sequence Analysis, RNA; D013025:Glycine max / Q000235:genetics*; D013312:Stress, Physiological; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant