title: Intranasal administration of live Lactobacillus species facilitates protection against influenza virus infection in mice.
abstract: Influenza virus infections continue to be a significant public health problem. For improved therapies and preventive measures against influenza, there has been an increased tendency in modern medicine involving the use of probiotics. In this study, we compared the protective efficacy of various live and dead Lactobacillus species against challenge with influenza virus in mice according to the administration route and dose. In addition, to understand the underlying mechanism behind this clinical protective effect, we performed immunologic assays including examination of IgA levels and cytokine profiles in the lung. The survival rate of mice receiving intranasal administration of Lactobacillus was higher than after oral administration, and administration of live bacteria was more protective than of dead bacteria. The lung levels of interleukin (IL)-12 and IgA were significantly increased (P<0.05). Conversely, the levels of the pro-inflammatory cytokines tumor necrosis factor-alpha and IL-6 were decreased. Interestingly, there were huge differences in protective effects of various Lactobacillus strains on influenza virus infection. Therefore, for clinical applications, selection of effective strains could be critical and individually optimized application regimens of the selected strains are required.
authors: Avian Disease Laboratory, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors: Ha-Na
authors: HN
authors: Youn
authors: Dong-Hun
authors: DH
authors: Lee
authors: Yu-Na
authors: YN
authors: Lee
authors: Jae-Keun
authors: JK
authors: Park
authors: Seong-Su
authors: SS
authors: Yuk
authors: Si-Yong
authors: SY
authors: Yang
authors: Hyun-Jeong
authors: HJ
authors: Lee
authors: Seo-Hyung
authors: SH
authors: Woo
authors: Hyoung-Moon
authors: HM
authors: Kim
authors: Joong-Bok
authors: JB
authors: Lee
authors: Seung-Yong
authors: SY
authors: Park
authors: In-Soo
authors: IS
authors: Choi
authors: Chang-Seon
authors: CS
authors: Song
mesh_terms: D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D002642:Chick Embryo; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007778:Lactobacillus; D008168:Lung / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000401:mortality / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D007070:Immunoglobulin A