title: A new expression vector for production of enzymes in the yeast Saccharomyces (Lachancea) kluyveri.
abstract: We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.
authors: Department of Biology, Lund University, Lund, Sweden. anna.rasmussen@biol.lu.se
authors: A
authors: A
authors: Rasmussen
authors: Y
authors: Y
authors: Lv
authors: K D
authors: KD
authors: Schnackerz
authors: J
authors: J
authors: Piškur
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004798:Enzymes / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D005822:Genetic Vectors / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D012440:Saccharomyces / Q000201:enzymology*
chemical_list: D004798:Enzymes; D005656:Fungal Proteins