title: Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.
abstract: Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.
authors: Menzies School of Health Research, Charles Darwin University, Darwin, Australia.
authors: Leisha J
authors: LJ
authors: Richardson
authors: Mirjam
authors: M
authors: Kaestli
authors: Mark
authors: M
authors: Mayo
authors: Jolene R
authors: JR
authors: Bowers
authors: Apichai
authors: A
authors: Tuanyok
authors: Jim
authors: J
authors: Schupp
authors: David
authors: D
authors: Engelthaler
authors: David M
authors: DM
authors: Wagner
authors: Paul S
authors: PS
authors: Keim
authors: Bart J
authors: BJ
authors: Currie
mesh_terms: D016957:Burkholderia pseudomallei / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis* / Q000382:microbiology; D057089:Pathology, Molecular / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial