title: The calvarial injection assay.
abstract: This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
authors: Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK. rvanthof@staffmail.ed.ac.uk
authors: Robert J
authors: RJ
authors: van 't Hof
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000627:therapeutic use; D001862:Bone Resorption / Q000188:drug therapy* / Q000473:pathology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007267:Injections; D053582:Interleukin-1alpha / Q000008:administration & dosage* / Q000627:therapeutic use; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D012886:Skull / Q000187:drug effects* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D055396:Bone Morphogenetic Protein 2; D053582:Interleukin-1alpha; D011994:Recombinant Proteins