title: Heart-type fatty acid-binding protein and its relation with morphological changes in rat myocardial damage model induced by isoproterenol.
abstract: We have investigated heart type fatty acid binding protein (H-FABP) rat serum values at different time point following subcutaneous (s.c) isoproterenol (ISO) administration and their correlation with severity of myocardial lesion. Thirty adult, male, Wistar rats were used for this study. Six rats per group were treated with a single dose of either ISO (ISO groups, dose 100 mg/kg, s.c.) at different time point (30', 60', 120', 240') or with saline (control group). Serum H-FABP was determined by enzyme-linked immunosorbent assay (ELISA) and histological analysis was performed by hematoxylin-eosin (HE) method of staining. The first serum H-FABP increase was obtained 30' following ISO administration, but maximal value was reached after 240'. Myocardial histological changes were time-dependent and correlated with serum H-FABP values (p<0.001). The results of the study suggest that H-FABP is sensitive marker for acute rat myocardial injury and its possible inclusion in myocardial injury screening studies in rats.
authors: Department of Medical Biochemistry, Faculty of Medicine, University of Sarajevo, Bosnia and Herzegovina. sabaheta.hasic@mf.unsa.ba
authors: Sabaheta
authors: S
authors: Hasić
authors: Radivoj
authors: R
authors: Jadrić
authors: Esad
authors: E
authors: Cosović
authors: Emina
authors: E
authors: Kiseljaković
authors: Zakira
authors: Z
authors: Mornjaković
authors: Mira
authors: M
authors: Winterhalter-Jadrić
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D009202:Cardiomyopathies / Q000097:blood / Q000139:chemically induced* / Q000473:pathology*; D004195:Disease Models, Animal; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins / Q000097:blood*; D006321:Heart / Q000187:drug effects; D007279:Injections, Subcutaneous; D007545:Isoproterenol / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015415:Biomarkers; C509948:FABP3 protein, rat; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D007545:Isoproterenol