title: Occurrence and molecular characterization of enteropathogenic Escherichia coli serotypes isolated from children with diarrhoea in Najaf, Iraq.
abstract: OBJECTIVE
  Enteropathogenic Escherichia coli (EPEC) are among the most important pathogens infecting children worldwide and are one of the main causes of diarrhoea. The study was carried out to investigate the occurrence of EPEC as a cause of infectious diarrhoea in children younger than 2 years of age and characterize their virulence genes.
  
  
  METHODS
  During the study period, a total of 656 faecal specimens from children with diarrhoea and 54 from healthy children were analyzed. E. coli isolates were serotypically identified with EPEC polyvalent and monovalent antisera. The isolated EPEC were examined for the presence of the attaching and effacing (eaeA), bundle-forming pilus (bfpA), Shiga like toxins (stx₁ and stx₂), enterohaemorrhagic E. coli enterohaemolysin (EHEC hlyA) and EPEC adherence factor (EAF) genes by the PCR assay.
  
  
  RESULTS
  The study has shown that 22 (3.4%) had diarrhoea due to EPEC, while no EPEC isolates were detected in asymptomatic children. The highest number of the EPEC isolated belonging to polyvalent 2. The primers encoding virulence genes were subjected to all the EPEC isolates. Only 9.1%, 27.3%, and 9.1% isolates gave positive re sults with intimin (eaeA), bfbA and (EAF) genes, respectively. None of the isolates were positive for stx₁, stx₂, and hlyA genes. Typical EPEC (eaeA⁺, bfpA⁺) was diagnosed in two isolates, while, atypical EPEC was manifested in four isolates.
  
  
  CONCLUSIONS
  According to the results, the frequency of EPEC isolates in Najaf was lower than what has been suspected and the investigation including the use of molecular technique and serotyping, are necessary to allow precise identification and epidemiological study of these pathogens.
authors: Department of Microbiology, College of Medicine, Kufa University, Iraq Najaf Kufa p.o. Box(18), Iraq. samirh@kuiraq.com
authors: Samer A
authors: SA
authors: Al Hilali
authors: Ali M
authors: AM
authors: Almohana
mesh_terms: D054308:Enteropathogenic Escherichia coli / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D007223:Infant; D007493:Iraq; D016133:Polymerase Chain Reaction; D012703:Serotyping; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors