title: Analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum Ca2+-ATPase contribution.
abstract: Ca(2+) signaling plays an essential role in several functions of cardiac myocytes. Transient rises and reductions of cytosolic Ca(2+), permitted by the sarcoplasmic reticulum Ca(2+) ATPase (SERCA2) and other proteins, control each cycle of contraction and relaxation. Here we provide a practical method for isolation of neonatal rat cardiac myocytes and measurement of Ca(2+) transients in cultured cardiac myocytes, yielding information on kinetic resolution of the transients, variations of cytosolic Ca(2+) concentrations, and adequacy of intracellular Ca(2+) stores. We also provide examples of experimental perturbations that can be used to assess the contribution of SERCA2 to Ca(2+) signaling.
authors: California Pacific Medical Center Research Institute, San Francisco, CA, USA.
authors: Anand Mohan
authors: AM
authors: Prasad
authors: Giuseppe
authors: G
authors: Inesi
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods; D056966:Excitation Contraction Coupling / Q000502:physiology; D032383:Myocytes, Cardiac / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D061251:Primary Cell Culture; D011817:Rabbits; D051381:Rats; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium