title: Characterization of the interaction between surfactants and enzymes by fluorescence probe.
abstract: In order to investigate the mechanism of the different stimulatory effects of the biosurfactant rhamnolipid and the chemical surfactant Tween 80 on enzymatic hydrolysis of lignocellulose, the interaction between surfactants and enzymes was analyzed by the fluorescence probe method using pyrene as probe. Based on the evolution law of pyrene fluorescence spectroscopy in the "surfactants-enzymes" systems, the interaction relationship between surfactants and enzymes was analyzed and discussed in this paper. The results show that enzyme molecules bind with rhamnolipid molecules, participate in the formation of rhamnolipid micelles, and increase the inner hydrophobic polarity of micelles, but do not change the properties of rhamnolipid micelles above the CMC (Critical Micelle Concentration). Nevertheless, for Tween 80, enzyme molecules also participate in the forming of micelles, however, they exhibit a stronger interaction with enzymes above the CMC. Both rhamnolipid and Tween 80 bind more strongly with xylanase than cellulase. Considering also previous experimental results, it can be concluded that the interaction between surfactants and enzymes improve enzyme stability and activity, and, therefore, the efficiency of enzymatic hydrolysis of lignocellulose is enhanced. The findings further provide theoretical knowledge about the mechanism of the stimulative effects of surfactants on enzymatic hydrolysis of lignocellulose.
authors: College of Environmental and Energy Engineering, Beijing University of Technology, Beijing, PR China. jialiu@bjut.edu.cn
authors: Jia
authors: J
authors: Liu
authors: Jingang
authors: J
authors: Shi
authors: Jian
authors: J
authors: Li
authors: Xingzhong
authors: X
authors: Yuan
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D011136:Polysorbates / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D006017:Glycolipids; D011136:Polysorbates; D011721:Pyrenes; D013501:Surface-Active Agents; C418382:rhamnolipid; C036909:lignocellulose; D008031:Lignin; C030984:pyrene; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases