title: In vitro myelotoxicity assessment of the emerging mycotoxins Beauvericin, Enniatin b and Moniliformin on human hematopoietic progenitors.
abstract: The aim of this study was to screen potential myelotoxicity of the emerging mycotoxins Beauvericin, Enniatin b and Moniliformin using human hematopoietic progenitor clonogenic assays. Depending on mycotoxins, inhibitory effects on proliferation of white blood cells progenitors (CFU-GM), platelet progenitors (CFU-MK) and red blood cells progenitors (BFU-E) have been detected at various concentrations. Beauvericin was cytotoxic at 32μM, 3.2μM and 6.4μM, had no effect on proliferation in the presence of 0.032μM, 0.16μM and 0.064μM, and the IC(50) was equal to 3.4μM, 0.7μM and 3.7μM for CFU-GM, CFU-MK and BFU-E, respectively. Enniatin b was cytotoxic at 6μM, 1.8μM and 5μM, had no effect on proliferation in the presence of 1μM, 1.1μM and 1.2μM and the IC(50) was equal to 4.4μM, 1.3μM and 3.3μM for CFU-GM, CFU-MK and BFU-E, respectively. Moniliformin was not cytotoxic at tested concentrations for CFU-GM and CFU-MK and cytotoxic at 10μM for BFU-E, had no effect on proliferation in the presence of 5μM, 0.1μM and 0.1μM and the IC(50) was equal to 31μM, 39μM and 4.1μM for CFU-GM, CFU-MK and BFU-E, respectively. Inhibition of the BFU-E differentiation has been observed in the presence of Enniatin b or Moniliformin. For the three mycotoxins, variation of distribution of CFU-MK colonies according to their size has been observed. These in vitro effects may be responsible for in vivo hematological troubles in case of consumption of contaminated commodities. In vivo studies have to be performed to test this hypothesis.
authors: Laboratoire de Toxicologie Alimentaire et Cellulaire, Université Européenne de Bretagne-Université de Bretagne Occidentale (UEB-UBO), 6 Av. Victor Le Gorgeu, CS93837, 29238 Brest Cedex 3, France.
authors: A S
authors: AS
authors: Ficheux
authors: Y
authors: Y
authors: Sibiril
authors: R
authors: R
authors: Le Garrec
authors: D
authors: D
authors: Parent-Massin
mesh_terms: D002478:Cells, Cultured; D003503:Cyclobutanes / Q000737:chemistry / Q000633:toxicity*; D047630:Depsipeptides / Q000737:chemistry / Q000633:toxicity*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D009183:Mycotoxins / Q000737:chemistry / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D003503:Cyclobutanes; D047630:Depsipeptides; D009183:Mycotoxins; C100264:enniatins; C004456:beauvericin; C008539:moniliformin