title: Antimutagenic and free radical scavenger effects of leaf extracts from Accacia salicina.
abstract: BACKGROUND
  Three extracts were prepared from the leaves of Accacia salicina; ethyl acetate (EA), chloroform (Chl) and petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative DNA damage protecting activity.
  
  
  METHODS
  Antioxidant activity of A. salicina extracts was determined by the ability of each extract to protect against plasmid DNA strand scission induced by hydroxyl radicals. An assay for the ability of these extracts to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 and TA 104 strains was conducted. In addition, nonenzymatic methods were employed to evaluate anti-oxidative effects of tested extracts.
  
  
  RESULTS
  These extracts from leaf parts of A. salicina showed no mutagenicity either with or without the metabolic enzyme preparation (S9). The highest protections against methylmethanesulfonate induced mutagenicity were observed with all extracts and especially chloroform extract. This extract exhibited the highest inhibitiory level of the Ames response induced by the indirect mutagen 2- aminoanthracene. All extracts exhibited the highest ability to protect plasmid DNA against hydroxyl radicals induced DNA damages. The ethyl acetate (EA) and chloroform (Chl) extracts showed with high TEAC values radical of 0.95 and 0.81 mM respectively, against the ABTS(.+).
  
  
  CONCLUSIONS
  The present study revealed the antimutagenic and antioxidant potenty of plant extract from Accacia salicina leaves.
authors: Department of Cellular and Molecular Biology, Faculty of Dental Medicine, Rue Avicenne,Monastir, 5000, Tunisia.
authors: Jihed
authors: J
authors: Boubaker
authors: Hedi Ben
authors: HB
authors: Mansour
authors: Kamel
authors: K
authors: Ghedira
authors: Leila
authors: L
authors: Chekir-Ghedira
mesh_terms: D000045:Acacia / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000378:metabolism*; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry; D010957:Plasmids / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts