title: Stem cell heterogeneity of mononucleated cells from murine peripheral blood: molecular analysis.
abstract: The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers. The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell types, that is, adherent and suspension. Following a total culture time of 14 days, adherent cells activated the Cd105 gene while suspension cells activated the Sca-1 gene. Both progenitor markers, Cbfa-1 and Ostf-1, were inactivated in both suspension and adherent cells after 14-day culture compared to cells cultured 3 days in designated differentiation medium. In conclusion, molecular analyses showed that primary mononucleated cells are heterogeneous, consisting of hematopoietic stem cells (suspension) and mesenchymal stem cells (adherent) while both cells contained no progenitor cells.
authors: Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 43600 Bangi, Malaysia.
authors: Muhammad Dain
authors: MD
authors: Yazid
authors: Shahrul Hisham
authors: SH
authors: Zainal Ariffin
authors: Sahidan
authors: S
authors: Senafi
authors: Zaidah
authors: Z
authors: Zainal Ariffin
authors: Rohaya
authors: R
authors: Megat Abdul Wahab
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics; D003470:Culture Media; D000071063:Endoglin; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000950:Antigens, Ly; D003470:Culture Media; D000071063:Endoglin; C502004:Eng protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; C074650:Ly6a protein, mouse; D008565:Membrane Proteins