title: Parallelized STED fluorescence nanoscopy.
abstract: We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.
authors: German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
authors: Pit
authors: P
authors: Bingen
authors: Matthias
authors: M
authors: Reuss
authors: Johann
authors: J
authors: Engelhardt
authors: Stefan W
authors: SW
authors: Hell
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity