title: Detoxifying response in juvenile tench fed by selenium diet.
abstract: The effects of a selenium (Se) diet (1.0 mg Se kg⁻¹) were investigated on growth, accumulation and antioxidant response in juvenile Tinca tinca at three endpoints (0, 4 and 8 weeks). Growth and condition factor (K>1.5) for both control (0.25 mg Se kg⁻¹) and Se tench were not significantly affected. Se exposed fish exhibited the highest Se level in the kidney and the liver after 4 weeks. By feeding more Se the accumulation capacity of tench did not increase and a plateau, mainly for the liver, was thus reached. Se level remained almost constant in the muscle if compared to own control and for each endpoint. Superoxide dismutase activity in both tissues was not affected by Se supplementation and the higher catalase level in the kidney might support the hypothesis that the enzyme was adequate to remove the hydrogen peroxide production following Se exposure. However, supplemented diet with higher Se level could be critical for tench, as it may cause a lowering of glutathione peroxidase and glutathione reductase activities facilitating the onset of oxidative damage. The enhancement of thiol level and glutathione S-transferase activity, mainly in the liver, could be the signals of the only protection against the oxidative damage induced by Se.
authors: Laboratory of Ecotoxicology, Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy.
authors: Nicole
authors: N
authors: Pacini
authors: Maria Cesarina
authors: MC
authors: Abete
authors: Ambrosius Josef Martin
authors: AJ
authors: Dörr
authors: Marino
authors: M
authors: Prearo
authors: Mauro
authors: M
authors: Natali
authors: Antonia Concetta
authors: AC
authors: Elia
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003530:Cyprinidae / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism*; D004032:Diet; D019587:Dietary Supplements; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage* / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016877:Oxidants; D013438:Sulfhydryl Compounds; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D012643:Selenium