title: Yeast two-hybrid screens: improvement of array-based screening results by N- and C-terminally tagged fusion proteins.
abstract: Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.
authors: Max-von-Pettenkofer-Institut, Lehrstuhl Virologie, Ludwig-Maximilians-Universität-LMU, München, Germany.
authors: Thorsten
authors: T
authors: Stellberger
authors: Roman
authors: R
authors: Häuser
authors: Peter
authors: P
authors: Uetz
authors: Albrecht von
authors: Av
authors: Brunn
mesh_terms: D003001:Cloning, Molecular; D014645:Herpesvirus 3, Human; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000254:growth & development
chemical_list: D011993:Recombinant Fusion Proteins; D014764:Viral Proteins