title: Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.
abstract: In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.
authors: Department of Molecular Pediatrics, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, Münich 80337, Germany. soeren.gersting@med.lmu.de
authors: Søren W
authors: SW
authors: Gersting
authors: Amelie S
authors: AS
authors: Lotz-Havla
authors: Ania C
authors: AC
authors: Muntau
mesh_terms: D002478:Cells, Cultured; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins