title: Glutathione S-transferase mu gene variants and colorectal cancer development--use of sequence-specific probes for an Iranian population.
abstract: BACKGROUND
  Enzymes of the glutathione S-transferase (GST) family are encoded by a set of polymorphic genes as an important part of cellular chemical defense. The aim of this study was to investigate the possible effect of GSTM1 deletion on susceptibility to developing clinical outcome of colorectal cancer in a group of CRC patients from Isfahan province, Iran, in comparison to age and gender matched control group.
  
  
  METHODS
  DNA was extracted from blood of 140 CRC patients and 90 healthy individuals and a set of sequence specific hybridization probes was used for GSTM1 genotyping by real-time PCR in Light-Cycler instrument. Chi-squared test was used to assess the statistical significance of observed differences between the patient and control subjects of different genders and ages. To estimate the risk for overall and stratified analyses, odds ratios (OR) with 95% confidence intervals (CI) computed with logistic regression.
  
  
  RESULTS
  No difference in GSTM1 null genotype frequency was found in CRC patients and controls stratified by gender (p value=0.14). The data were suggested a trend of increasing risk for GSTM1 null genotype in patients over 60 years old compared with controls (p value=0.05). GSTM1 null genotype carried an increase of the odds of developing CRC in patients over 60 years old (OR=2.7; 95% CI: 1.03-7.05). No significant association was found (P>0.05) between the GSTM1 null genotype with tumor site (right, left, rectum) or tumor differentiation (well, moderately).
  
  
  CONCLUSIONS
  Our findings suggest that the GSTM1 null genotype may contribute to colorectal cancer development in people over 60 years old.
authors: Department of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Monireh
authors: M
authors: Aghajany-Nasab
authors: Mojtaba
authors: M
authors: Panjehpour
authors: Siamak Mirab
authors: SM
authors: Samiee
authors: Farzaneh
authors: F
authors: Rahimi
authors: Ahmad
authors: A
authors: Movahedian
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D015342:DNA Probes / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: D015342:DNA Probes; D005982:Glutathione Transferase