title: A potential nanomedicine consisting of heparin-loaded polysaccharide nanocarriers for the treatment of asthma.
abstract: A new nanomedicine consisting of chitosan/carboxymethyl-β-cyclodextrin loaded with unfractioned or low-molecular-weight heparin is described and its potential in asthma treatment is evaluated. The nanoparticles are prepared by ionotropic gelation showing a size that between 221 and 729 nm with a positive zeta potential. The drug association efficiency is higher than 70%. Developed nanosystems are stable in Hank's balanced salt solution at pH = 6.4, releasing the drug slowly. Ex vivo assays show that nanocarriers lead to an improvement of heparin preventing mast cell degranulation. These results agree with the effective cellular internalization of the fluorescently labeled nanocarriers, and suggest these nanomedicines as promising formulations for asthma treatment.
authors: Department of Pharmacy, Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain.
authors: Felipe A
authors: FA
authors: Oyarzun-Ampuero
authors: Jose
authors: J
authors: Brea
authors: Maria I
authors: MI
authors: Loza
authors: Maria J
authors: MJ
authors: Alonso
authors: Dolores
authors: D
authors: Torres
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015550:Cell Degranulation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis*; D004339:Drug Compounding / Q000379:methods; D005260:Female; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry / Q000494:pharmacology*; D006632:Histamine / Q000096:biosynthesis; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008970:Molecular Weight; D050997:Nanomedicine / Q000379:methods*; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D006495:Heparin, Low-Molecular-Weight; D047392:beta-Cyclodextrins; C441457:carboxymethyl-beta-cyclodextrin; D006632:Histamine; D048271:Chitosan