title: Chromatin immunoprecipitation in mouse hippocampal cells and tissues.
abstract: Chromatin immunoprecipitation (ChIP) has been developed for studying protein-DNA interactions and has been extensively used for mapping the localization of posttranslationally modified histones, histone variants, transcription factors, or chromatin modifying enzymes at a given locus or on a genome-wide scale. ChIP methods have been modified and improved over the years to fit a variety of different cell types and tissues. Here, we present a detailed protocol for hippocampal ChIP, of both minced tissue and enzyme-separated hippocampal cells. This protocol enables to study chromatin-protein interactions in a specified population of hippocampal cells, allowing to study chromatin regulation in the central nervous system in a variety of conditions and disorders. Our assay has been developed for histone modifications but is suited for any chromatin binding protein for which specific ChIP-grade antibodies are available.
authors: Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors: Badi Sri
authors: BS
authors: Sailaja
authors: Takumi
authors: T
authors: Takizawa
authors: Eran
authors: E
authors: Meshorer
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D051379:Mice; D011499:Protein Processing, Post-Translational
chemical_list: D002843:Chromatin; D006657:Histones