title: Biodegradation of all-in-one self-etch adhesive systems at the resin-dentin interface.
abstract: This study evaluated the effects of two exogenous enzymes on the resin-dentin interface. Collagenase (Col) and acetylcholinesterase (Ach) were used to simulate salivary enzymes and accelerate the aging process of the bonding interfaces. Four adhesives, Adper Single Bond 2 (SB), Clearfil SE Bond (SE), Clearfil tri-S Bond (S3) and G-Bond (G), were bonded to the dentin surfaces. After storage in water with collagenase or acetylcholinesterase, the specimens were examined using a microtensile bond strength test (MTBS). Nanoleakage patterns were observed with a scanning electron microscope (SEM). The MTBS results demonstrated significantly lower bond strengths in the groups stored with either enzyme than in water. SB exhibited severe degradation after exposure to collagenase, while G showed severe degradation after exposure to acetylcholinesterase. All of the self-etch systems (SE, S3 and G) exhibited water-tree patterns within the adhesive layer when immersed in water for three months. The etch-and-rinse system (SB) showed nanoleakage within the hybrid layer and the adhesive.
authors: Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University.
authors: Sirichan
authors: S
authors: Chiaraputt
authors: Pitchaya
authors: P
authors: Roongrujimek
authors: Vanthana
authors: V
authors: Sattabanasuk
authors: Nurot
authors: N
authors: Panich
authors: Choltacha
authors: C
authors: Harnirattisai
authors: Pisol
authors: P
authors: Senawongse
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D000269:Adhesives / Q000737:chemistry*; D017364:Collagenases / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry*; D003738:Dental Cements / Q000737:chemistry; D045662:Dental Etching; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000601:surgery; D019279:Resin Cements / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D013718:Tensile Strength
chemical_list: D000269:Adhesives; C554641:Adper single bond 2; C438313:Clearfil SE Bond; C515604:Clearfil Tri-S Bond; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; D019279:Resin Cements; D012117:Resins, Synthetic; D000110:Acetylcholinesterase; D017364:Collagenases