title: Origin of initial burst in activity for Trichoderma reesei endo-glucanases hydrolyzing insoluble cellulose.
abstract: The kinetics of cellulose hydrolysis have long been described by an initial fast hydrolysis rate, tapering rapidly off, leading to a process that takes days rather than hours to complete. This behavior has been mainly attributed to the action of cellobiohydrolases and often linked to the processive mechanism of this exo-acting group of enzymes. The initial kinetics of endo-glucanases (EGs) is far less investigated, partly due to a limited availability of quantitative assay technologies. We have used isothermal calorimetry to monitor the early time course of the hydrolysis of insoluble cellulose by the three main EGs from Trichoderma reesei (Tr): TrCel7B (formerly EG I), TrCel5A (EG II), and TrCel12A (EG III). These endo-glucanases show a distinctive initial burst with a maximal rate that is about 5-fold higher than the rate after 5 min of hydrolysis. The burst is particularly conspicuous for TrCel7B, which reaches a maximal turnover of about 20 s(-1) at 30 °C and conducts about 1200 catalytic cycles per enzyme molecule in the initial fast phase. For TrCel5A and TrCel12A the extent of the burst is 2-300 cycles per enzyme molecule. The availability of continuous data on EG activity allows an analysis of the mechanisms underlying the initial kinetics, and it is suggested that the slowdown is linked to transient inactivation of enzyme on the cellulose surface. We propose, therefore, that the frequency of structures on the substrate surface that cause transient inactivation determine the extent of the burst phase.
authors: Roskilde University, NSM, Biomaterials, 1 Universitetsvej, DK-4000 Roskilde, Denmark.
authors: Leigh
authors: L
authors: Murphy
authors: Nicolaj
authors: N
authors: Cruys-Bagger
authors: Heidi Delcomyn
authors: HD
authors: Damgaard
authors: Martin J
authors: MJ
authors: Baumann
authors: Søren Nymand
authors: SN
authors: Olsen
authors: Kim
authors: K
authors: Borch
authors: Søren Flensted
authors: SF
authors: Lassen
authors: Matt
authors: M
authors: Sweeney
authors: Hirosuke
authors: H
authors: Tatsumi
authors: Peter
authors: P
authors: Westh
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006868:Hydrolysis; D012995:Solubility; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase