title: Individual bioaerosol particle discrimination by multi-photon excited fluorescence.
abstract: Femtosecond laser induced multi-photon excited fluorescence (MPEF) from individual airborne particles is tested for the first time for discriminating bioaerosols. The fluorescence spectra, analysed in 32 channels, exhibit a composite character originating from simultaneous two-photon and three-photon excitation at 790 nm. Simulants of bacteria aggregates (clusters of dyed polystyrene microspheres) and different pollen particles (Ragweed, Pecan, Mulberry) are clearly discriminated by their MPEF spectra. This demonstration experiment opens the way to more sophisticated spectroscopic schemes like pump-probe and coherent control.
authors: Université de Genève, GAP-Biophotonics, Rue de l’Ecole de Médecine 20, 1211 Geneva 4, Switzerland. Denis.Kiselev@unige.ch
authors: Denis
authors: D
authors: Kiselev
authors: Luigi
authors: L
authors: Bonacina
authors: Jean-Pierre
authors: JP
authors: Wolf
mesh_terms: D000336:Aerosols / Q000032:analysis*; D000388:Air / Q000032:analysis*; D000393:Air Pollutants / Q000032:analysis*; D001688:Biological Products / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D052638:Particulate Matter / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D001688:Biological Products; D052638:Particulate Matter