title: Medium optimization for enhanced co-production of two bioactive metabolites in the same fermentation by a statistical approach.
abstract: This paper describes improved optimization method that combines the one-factor-at-a-time method (OFAT), Plackett-Burman design, and the response surface method (RSM), which were used to optimize the medium for the production of fumigaclavine C (FC) and helvolic acid (HA) from endophytic Aspergillus fumigatus CY018 simultaneously. The ideal carbon and nitrogen sources for the two compounds were assessed initially via the one-factor-at-a-time method. Three key cultivation factors (pH, phosphate, and inoculum size) were chosen based on the results of Plackett-Burman design, and subsequently optimized by the central composite design. The two metabolites were amply afforded when the cultivation was carried out with the inoculum size of 2.45% at pH 4.2 and 28°C for 19 days in the medium containing (g/l): mannitol 50, sodium succinate 5.4, NaNO₃ 2, MgSO₄·7H₂O 0.3, FeSO₄·7H₂O 0.01, and KH₂PO₄ 0.67. The highest yields of FC and HA achieved herein were 17.26 and 16.88 mg/l. This work might be the first endeavor leading to the improved simultaneous production of two complex active metabolites with a single strain.
authors: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors: Qi
authors: Q
authors: Wu
authors: Yong-Chun
authors: YC
authors: Song
authors: Hao
authors: H
authors: Xu
authors: Ye
authors: Y
authors: Guo
authors: Jing
authors: J
authors: Li
authors: Ren-Xiang
authors: RX
authors: Tan
mesh_terms: D000465:Algorithms; D000818:Animals; D001232:Aspergillus fumigatus / Q000737:chemistry*; D001688:Biological Products / Q000378:metabolism; D003470:Culture Media; D004876:Ergot Alkaloids / Q000096:biosynthesis* / Q000737:chemistry; D005285:Fermentation; D005672:Fusidic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D026121:Indole Alkaloids / Q000737:chemistry; D008956:Models, Chemical; D015394:Molecular Structure; D009584:Nitrogen / Q000378:metabolism
chemical_list: D001688:Biological Products; D003470:Culture Media; D004876:Ergot Alkaloids; D026121:Indole Alkaloids; C492995:fumigaclavine C; D005672:Fusidic Acid; C011267:helvolic acid; D009584:Nitrogen