title: Involvement of SHP2 in focal adhesion, migration and differentiation of neural stem cells.
abstract: OBJECTIVE
  SHP2 (Src-homology-2 domain-containing protein tyrosine phosphatase) plays an important role in cell adhesion, migration and cell signaling. However, its role in focal adhesion, differentiation and migration of neural stem cells is still unclear.
  
  
  METHODS
  In this study, rat neurospheres were cultured in suspension and differentiated neural stem cells were cultured on collagen-coated surfaces.
  
  
  RESULTS
  The results showed that p-SHP2 co-localized with focal adhesion kinase (FAK) and paxillin in neurospheres and in differentiated neural precursor cells, astrocytes, neurons, and oligodendrocytes. Suppression of SHP2 activity by PTP4 or siRNA-mediated SHP2 silencing caused reduction in the cell migration and neurite outgrowth, and thinning of glial cell processes. Differentiation-induced activation of FAK, Src, paxillin, ERK1/2, and RhoA was decreased by SHP2 inactivation.
  
  
  CONCLUSIONS
  These results indicate that SHP2 is recruited in focal adhesions of neural stem cells and regulates focal adhesion formation. SHP2-mediated regulation of neural differentiation and migration may be related to formation of focal adhesions and RhoA and ERK1/2 activation.
authors: Department of Anatomy, Faculty of Medicine, Chung Shan Medical University, Taichung, Taiwan, ROC.
authors: Yuahn-Sieh
authors: YS
authors: Huang
authors: Cheng-Yi
authors: CY
authors: Cheng
authors: Sheau-Huei
authors: SH
authors: Chueh
authors: Dueng-Yuan
authors: DY
authors: Hueng
authors: Yu-Fen
authors: YF
authors: Huang
authors: Chun-Ming
authors: CM
authors: Chu
authors: Sheng-Tang
authors: ST
authors: Wu
authors: Ming-Cheng
authors: MC
authors: Tai
authors: Chang-Min
authors: CM
authors: Liang
authors: Mei-Hsiu
authors: MH
authors: Liao
authors: Chia-Chieh
authors: CC
authors: Chen
authors: Lie-Hang
authors: LH
authors: Shen
authors: Kuo-Hsing
authors: KH
authors: Ma
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D022001:Focal Adhesions / Q000378:metabolism; D007150:Immunohistochemistry; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11