title: Antibacterial effect and cytocompatibility of nano-structured TiO2 film containing Cl.
abstract: The aim of this study was to investigate the antibacterial effect and cytocompatibility of a nano-structured TiO2 film that contained Cl and had been coated onto commercially pure titanium. First, we prepared nano-structured TiO2 by anodization with hydrofluoric acid. Then, to confer an antibacterial effect, we performed a second anodization with NaCl solutions of different concentrations (0.5 M, 1 M, 2 M). The morphology, composition, and wettability of the surface were investigated by SEM, EDS, and a video contact angle measuring system. The antibacterial effect was evaluated by film adhesion method. And cytotoxicity was determined by the viability of MG-63 cells in a MTT assay. The SEM and EDS results showed that the TiO2 nano-structure containing Cl had successfully formed after the second anodization. The contact angle analysis showed that the anodized titanium had a hydrophilic character. The results of this in vitro investigation demonstrated that the TiO2 nano-structure film anodized in 1 M NaCl had an antibacterial effect and good cell compatibility.
authors: BK21, Department and Research Institute of Dental Biomaterials and Bioengineering, and Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University.
authors: Min-Kyung
authors: MK
authors: Kang
authors: Seung-Kyun
authors: SK
authors: Moon
authors: Kwang-Mahn
authors: KM
authors: Kim
authors: Kyoung-Nam
authors: KN
authors: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015921:Dental Implants; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D010006:Osteoblasts / Q000187:drug effects; D012965:Sodium Chloride / Q000737:chemistry; D013295:Streptococcus mutans / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D017155:Wettability
chemical_list: D000900:Anti-Bacterial Agents; D015921:Dental Implants; C009495:titanium dioxide; D012965:Sodium Chloride; D014025:Titanium