title: Effects of two chronic stress models on ligature-induced periodontitis in Wistar rats.
abstract: OBJECTIVE
  The present study aims at comparing the effects of physical and variable chronic stress on ligature-induced periodontitis in rats.
  
  
  METHODS
  Forty-eight adult Wistar rats were randomly assigned to four groups (n=12): physical stress, variable stress, positive control and negative control. The models of physical stress were immobilization and immobilization associated with exposure to cold. The models of variable stress were exposure to intermittent light, 24h isolation, oral cavity examination, crowded environment, smell of blood and noise. After 10 days of physical or variable stress animals underwent experimental induction of periodontal disease in one oral side. Positive control also underwent experimental induction of periodontal disease on the 10th day. Negative control did not receive any type of intervention. At the end of the experimental period (60 days), all animals were euthanized. After routine laboratory processing, images of the histological sections were digitised and submitted to histometric measurement using two parameters: histologic attachment loss and bone loss.
  
  
  RESULTS
  Histological attachment loss and bone loss were greater (p<0.05) in the physical stress group than in the other groups (variable stress, positive and negative control groups). On the non-periodontitis side, these same histological parameters did not significantly differ amongst groups.
  
  
  CONCLUSIONS
  Physical stress negatively modulated the response pattern to experimentally induced periodontitis in rats.
authors: Dental School, University of Cuiabá, Brazil.
authors: Alex
authors: A
authors: Semenoff-Segundo
authors: Alessandra Nogueira
authors: AN
authors: Porto
authors: Tereza Aparecida Dele Vedove
authors: TA
authors: Semenoff
authors: Jose Roberto
authors: JR
authors: Cortelli
authors: Fernando Oliveira
authors: FO
authors: Costa
authors: Sheila Cavalca
authors: SC
authors: Cortelli
authors: Alvaro Francisco
authors: AF
authors: Bosco
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology; D000704:Analysis of Variance; D000818:Animals; D002908:Chronic Disease; D003080:Cold Temperature; D004195:Disease Models, Animal; D004777:Environment; D007103:Immobilization; D008026:Ligation; D008027:Light; D010518:Periodontitis / Q000209:etiology* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D012903:Smell; D012934:Social Isolation; D013312:Stress, Physiological / Q000502:physiology*; D013315:Stress, Psychological / Q000503:physiopathology*