title: Generation of human osteoclasts from peripheral blood.
abstract: Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.
authors: Nordic Bioscience A/S, Herlev, Denmark. kh@nordicbioscience.com
authors: Kim
authors: K
authors: Henriksen
authors: Morten A
authors: MA
authors: Karsdal
authors: Adam
authors: A
authors: Taylor
authors: Denise
authors: D
authors: Tosh
authors: Fraser P
authors: FP
authors: Coxon
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D058621:Blood Buffy Coat / Q000166:cytology*; D001862:Bone Resorption; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D003094:Collagen / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000032:analysis; D007527:Isoenzymes / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology; D008264:Macrophages / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010012:Osteogenesis; D053245:RANK Ligand / Q000276:immunology; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D039302:Integrin alphaVbeta3; D007527:Isoenzymes; D018950:Lipopolysaccharide Receptors; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase