title: Novel docetaxel-loaded nanoparticles based on PCL-Tween 80 copolymer for cancer treatment.
abstract: BACKGROUND
  The formulation of docetaxel available for clinical use (Taxotere) contains a high concentration of polysorbate 80 (Tween 80). After incorporation of Tween 80 into poly-ɛ-caprolactone (PCL)-Tween 80 copolymer, the relative amount of Tween 80 should be decreased and the advantages of PCL and Tween 80 should be combined.
  
  
  METHODS
  A novel PCL-Tween 80 copolymer was synthesized from ɛ-caprolactone and Tween 80 in the presence of stannous octoate as a catalyst via ring opening polymerization. Two types of nanoparticle formulation were made from commercial PCL and a self-synthesized PCL-Tween 80 copolymer using a modified solvent extraction/evaporation method.
  
  
  RESULTS
  The nanoparticles were found by field emission scanning electron microscopy to have a spherical shape and be 200 nm in diameter. The copolymers could encapsulate 10% of the drug in the nanoparticles and release 34.9% of the encapsulated drug over 28 days. PCL-Tween 80 nanoparticles could be internalized into the cells and had higher cellular uptake than the PCL nanoparticles. The drug-loaded PCL-Tween 80 nanoparticles showed better in vitro cytotoxicity towards C6 cancer cells than commercial Taxotere at the same drug concentration.
  
  
  CONCLUSIONS
  Nanoparticles using PCL-Tween 80 copolymer as drug delivery vehicles may have a promising outcome for cancer patients.
authors: School of Life Sciences, Tsinghua University, Beijing, People's Republic of China.
authors: Yuandong
authors: Y
authors: Ma
authors: Yi
authors: Y
authors: Zheng
authors: Xiaowei
authors: X
authors: Zeng
authors: Liqin
authors: L
authors: Jiang
authors: Hongbo
authors: H
authors: Chen
authors: Ranyi
authors: R
authors: Liu
authors: Laiqiang
authors: L
authors: Huang
authors: Lin
authors: L
authors: Mei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002470:Cell Survival; D003374:Coumarins / Q000737:chemistry; D000077143:Docetaxel; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D043823:Taxoids / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D003374:Coumarins; D053769:Nanocapsules; D011091:Polyesters; D011136:Polysorbates; D043823:Taxoids; D013844:Thiazoles; C517282:coumarin 6; D000077143:Docetaxel; C016240:polycaprolactone