title: Optimization of triplex real time PCR for detecting Staphylococcus aureus mecA, pvl and nuc genes.
abstract: Multiplex polymerase chain reaction (PCR) allows simultaneous detection of two or more genes, using the same reaction conditions, and so it is possible the rapid detection of methicillin resistant Staphylococcus aureus strains (MRSA) in clinical specimens. This study aimed to implement, for the first time in our laboratory, a triplex real time PCR (RT-PCR) technique for detection of genes encoding resistance to oxacillin and synthesis of Panton Valentine leukocidin (pvl), a pathogenicity factor characteristic for community acquired strains (CA-MRSA). The application of this method will permit the epidemiological surveillance of circulating strains and early application of prevention measures.
authors: Gr. T. Popa University of Medicine and Pharmacy, Iaşi, Microbiology Department.
authors: Teodora
authors: T
authors: Vremeră
authors: Luminiţa Smaranda
authors: LS
authors: Iancu
authors: Cătălina
authors: C
authors: Logigan
authors: Eduard
authors: E
authors: Năstase
authors: Egidia
authors: E
authors: Miftode
authors: Cătălina
authors: C
authors: Luncă
authors: Olivia
authors: O
authors: Dorneanu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D007956:Leukocidins / Q000235:genetics*; D008836:Micrococcal Nuclease / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D046915:Penicillin-Binding Proteins; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; C074002:nuc protein, staphylococcus; D008836:Micrococcal Nuclease