title: Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells.
abstract: A linear relationship was found between the antibody production rate (q(mAb)) and the glucose and lactate consumption rate (q(GL)) in Chinese hamster ovary cells. Under a series of q(mAb)-perturbing conditions, q(GL) was determined and a linear relationship between q(mAb) and q(GL) was further established (R(2) = 0.914). Mitochondrial dehydrogenase activity was monitored in all the q(mAb)-perturbing conditions and showed a linear correlation with q(GL) (R(2) = 0.874) as well as with q(mAb). Taken collectively, our results establish that the metabolic parameter, q(GL), is linearly correlated with q (mAb); this finding strengthens our current understanding of process optimization for antibody production.
authors: The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. cathy_chenfei@126.com
authors: Fei
authors: F
authors: Chen
authors: Zhaoyang
authors: Z
authors: Ye
authors: Liang
authors: L
authors: Zhao
authors: Xuping
authors: X
authors: Liu
authors: Li
authors: L
authors: Fan
authors: Wen-Song
authors: WS
authors: Tan
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D019344:Lactic Acid; D010088:Oxidoreductases; D005947:Glucose