title: In vitro effect of adenosine A2A receptor antagonist SCH 442416 on the             expression of glutamine synthetase and glutamate aspartate transporter in rat             retinal Müller cells at elevated hydrostatic pressure.
abstract: The aim of this study was to investigate the effect of an adenosine A2A             receptor antagonist on the expression of glutamine synthetase (GS) and glutamate             aspartate transporter (GLAST) in rat retinal Müller cells at elevated hydrostatic             pressure in vitro. Immunofluorescence staining of GS and GFAP was used for the             identification of Müller cells. The expression of GS and GLAST in different hydrostatic             pressure (0, 20, 40, 60, 80 mmHg/24 h) was examined by real-time PCR and Western             blotting to identify the most suitable pressure. Müller cells treated with 0.1,             1, 10 µM SCH 442416 (A2A receptor antagonist) in the most suitable pressure, and             the levels of GS and GLAST were examined by real-time PCR and Western blotting.             Significantly increased expression of GS and GLAST at 40 mmHg pressure was observed             in Müller cells and treatment with 10 µM SCH 442416 in 40 mmHg pressure further             promoted the expression of GS and GLAST. A2A receptor antagonist increased the             expression of GLAST and GS of Müller cells and accelerated the clearance of extracellular             glutamate.
authors: Department of Ophthalmology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors: Jun
authors: J
authors: Yu
authors: Yisheng
authors: Y
authors: Zhong
authors: Xi
authors: X
authors: Shen
authors: Yu
authors: Y
authors: Cheng
authors: Jin
authors: J
authors: Qi
authors: Jun
authors: J
authors: Wang
mesh_terms: D058917:Adenosine A2 Receptor Antagonists / Q000494:pharmacology*; D027322:Amino Acid Transport System X-AG / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis* / Q000235:genetics; D018698:Glutamic Acid / Q000378:metabolism; D006874:Hydrostatic Pressure; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043705:Receptor, Adenosine A2A / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D058917:Adenosine A2 Receptor Antagonists; D027322:Amino Acid Transport System X-AG; D011720:Pyrazoles; D011743:Pyrimidines; D043705:Receptor, Adenosine A2A; D018698:Glutamic Acid; D005974:Glutamate-Ammonia Ligase; C416650:SCH 442416