title: Cell proliferation of esophageal squamous epithelium in erosive and non-erosive reflux disease.
abstract: OBJECTIVE
  To elucidate cell proliferation in erosive reflux disease (ERD) and non-erosive reflux disease (NERD), we evaluated markers in squamous epithelial cells.
  
  
  METHODS
  Thirty-four consecutive patients with gastroesophageal-reflux-disease-related symptoms (21 NERD and 13 ERD) were evaluated for the enrolment into the study. All patients underwent 24-h pH monitoring, standard endoscopy, and biopsy for histological evaluation. The expression of cyclins D and A was evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) from isolated epithelial cells. In all samples, analysis of the isolated cell population revealed the presence of epithelial cells only.
  
  
  RESULTS
  Real-time RT-PCR showed that, in patients with ERD, the relative expression of cyclin D1 mRNA in esophageal epithelium was strongly decreased in comparison with NERD patients. The mean value of relative expression of cyclin D1 mRNA in NERD patients was 3.44 ± 1.9, whereas in ERD patients, it was 1.32 ± 0.87 (P = 0.011). Real-time RT-PCR showed that, in patients with ERD, relative expression of cyclin A mRNA in esophageal epithelium was decreased in comparison with that in NERD patients (2.31 ± 2.87 vs 0.66 ± 1.11). The mean bromodeoxyuridine labeling index in the NERD patients was 5.42% ± 1.68%, whereas in ERD patients, it was 4.3% ± 1.59%.
  
  
  CONCLUSIONS
  We confirmed reduced epithelial proliferation in ERD compared with NERD patients, and that individuals who develop ERD are characterized by weaker epithelial cell proliferation.
authors: Department of Clinical Medicine, University of Bologna, 40138 Bologna, Italy. carlo.calabrese2@unibo.it
authors: Carlo
authors: C
authors: Calabrese
authors: Lorenzo
authors: L
authors: Montanaro
authors: Giuseppina
authors: G
authors: Liguori
authors: Elisa
authors: E
authors: Brighenti
authors: Mauela
authors: M
authors: Vici
authors: Paolo
authors: P
authors: Gionchetti
authors: Fernando
authors: F
authors: Rizzello
authors: Massimo
authors: M
authors: Campieri
authors: Massimo
authors: M
authors: Derenzini
authors: Davide
authors: D
authors: Trerè
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D049109:Cell Proliferation; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism; D056741:Cyclin D / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology / Q000502:physiology*; D050782:Esophageal pH Monitoring; D004945:Esophagoscopy; D004947:Esophagus / Q000166:cytology* / Q000473:pathology*; D005764:Gastroesophageal Reflux / Q000473:pathology*; D006801:Humans
chemical_list: D015415:Biomarkers; D019925:Cyclin A; D056741:Cyclin D