title: In vitro susceptibility to different topical ophthalmic antibiotics of bacterial isolates from patients with conjunctivitis.
abstract: OBJECTIVE
  To test in vitro the susceptibility to different commercially-available topical antibiotics of isolates from patients with conjunctivitis, evaluated at the Microbiology Service of Turin Ophthalmic Hospital between January 2005-February 2007.
  
  
  METHODS
  Bacterial isolates were cultured using both liquid and solid media. The four most frequent isolates were analyzed and their in vitro susceptibility to levofloxacin, ofloxacin, norfloxacin, lomefloxacin, tobramycin, netilmycin, ampicillin, and chloramphenicol was tested, using the Kirby-Bauer diffusion method and National Committee for Clinical Laboratory Standards (NCCLS) serum standards.
  
  
  RESULTS
  The four most common bacteria isolates were: Staphylococcus aureus (27%), viridians Streptococci (22%), Staphylococcus epidermidis (16%) and Streptococcus pneumoniae (13%). The in vitro susceptibility to levofloxacin was highest (p<0.01) for all bacterial isolates.
  
  
  CONCLUSIONS
  In vitro susceptibility tests showed that levofloxacin had the highest cumulative efficacy against bacterial isolates. Netilmycin showed the highest efficacy against staphylococcus aureus, the most common bacterial isolate in this study.
authors: Department of Diagnosis and Laboratory Analysis, Ophthalmic Hospital, Turin, Italy.
authors: Franco
authors: F
authors: Giardini
authors: Giuseppe
authors: G
authors: Grandi
authors: Ugo
authors: U
authors: De Sanctis
authors: Chiara
authors: C
authors: Eandi
authors: Federica
authors: F
authors: Machetta
authors: Cristina
authors: C
authors: Pollino
authors: Federico Maria
authors: FM
authors: Grignolo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D003234:Conjunctivitis, Bacterial / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007558:Italy; D008826:Microbial Sensitivity Tests; D012189:Retrospective Studies; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000302:isolation & purification; D034363:Viridans Streptococci / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents