title: Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization.
abstract: Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from Triticum aestivum cv. 'Chinese Spring' (CS) × Dasypyrum villosum(Dv) intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from Dv were apparently added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76%, and 1.26% in CS×V58, CS×V59, and CS×V60, respectively). Molecular investigation indicated the presence of Dv DNA in the ILs. AFLP analysis of genomic DNA from the ILs, CS, and Dv detected a total of 120 polymorphic bands, of which 7 (5.8%) were present in some or all the ILs and Dv but were absent in CS. PCR amplification, sequence analysis of amplicons, and Southern blot hybridization confirmed the presence of Dv-specific sequences in each of the ILs. These results indicate cryptic introgression of Dv DNA sequences into the genome of the ILs. Some implications of this finding are discussed.
authors: Dipartimento di Biologia Cellulare e Ambientale, Sezione di Biologia Cellulare e Molecolare, Università di Perugia, Italia. cionini@unipg.it
authors: M E
authors: ME
authors: Caceres
authors: F
authors: F
authors: Pupilli
authors: M
authors: M
authors: Ceccarelli
authors: P
authors: P
authors: Vaccino
authors: V
authors: V
authors: Sarri
authors: C
authors: C
authors: De Pace
authors: P G
authors: PG
authors: Cionini
mesh_terms: D032461:Chromosomes, Plant; D003582:Cytogenetics / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D006824:Hybridization, Genetic; D010641:Phenotype; D017422:Sequence Analysis, DNA / Q000379:methods; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant