title: Efficacy of QCDCR formulated CpG ODN 2007 in Nile tilapia against Streptococcus iniae and identification of upregulated genes.
abstract: The potential of using a QCDCR (quilA:cholesterol:dimethyl dioctadecyl ammonium bromide:carbopol:R1005 glycolipid) formulated CpG oligodeoxynucleotide (ODN), ODN 2007, to confer protection in Nile tilapia against Streptococcus iniae infection was evaluated in this study. At two days post treatment, QCDCR formulated ODN 2007 elicited significant (P<0.05) protection to Nile tilapia, with relative percent survival of 63% compared to fish treated by QCDCR alone. To understand the molecular mechanisms involved in the protective immunity elicited by ODN 2007, suppression subtractive cDNA hybridization technique was used to identify upregulated genes induced by ODN 2007. A total of 69 expressed sequence tags (ESTs) were identified from the subtractive cDNA library. Quantitative PCR revealed that 44 ESTs were significantly (P<0.05) upregulated by ODN 2007, including 29 highly (>10-fold) and 15 moderately (<10-fold) upregulated ESTs. Of all ESTs, putative peroxisomal sarcosine oxidase was upregulated the highest. The 69 ESTs only included six genes that had putative functions related to immunity, of which only two (putative glutaredoxin-1 and carboxypeptidase N catalytic chain) were confirmed to be significantly upregulated. Our results suggest that the protection elicited by ODN 2007 is mainly through innate immune responses directly or indirectly related to immunity.
authors: Aquatic Animal Health Research Unit, USDA-ARS, 990 Wire Road, Auburn, AL 36832, USA. Julia.Pridgeon@ars.usda.gov
authors: Julia W
authors: JW
authors: Pridgeon
authors: Phillip H
authors: PH
authors: Klesius
authors: Xingjiang
authors: X
authors: Mu
authors: Robert J
authors: RJ
authors: Yancey
authors: Michele S
authors: MS
authors: Kievit
authors: Paul J
authors: PJ
authors: Dominowski
mesh_terms: D000818:Animals; D023681:Cichlids / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018899:CpG Islands; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D007109:Immunity / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D050879:Sarcosine Oxidase / Q000235:genetics / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary*; D013291:Streptococcus / Q000276:immunology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D050879:Sarcosine Oxidase