title: Detection of calcium release via ryanodine receptors.
abstract: The ryanodine receptor ion channels (RyRs) release Ca(2+) from the endo/sarcoplasmic reticulum in a variety of nonvertebrate and vertebrate species including flies, crustaceans, birds, fish, and amphibians. They are most abundant in skeletal and cardiac muscle, where in response to an action potential, the release of Ca(2+) ions from the sarcoplasmic reticulum through the RyRs into the cytoplasm leads to muscle contraction (i.e., excitation-contraction coupling). Here, we describe how to determine their cellular location using isoform-specific antibodies, their protein levels using an in vitro ((3)H)ryanodine-binding assay, and their cellular release of Ca(2+) using RyR-specific channel agonists and inhibitors.
authors: Department of Medicine, Division of Pulmonary, Allergy and Critical Care, Duke University, Durham, NC, USA.
authors: Jerry P
authors: JP
authors: Eu
authors: Gerhard
authors: G
authors: Meissner
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D009132:Muscles / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000032:analysis / Q000276:immunology; D021381:Protein Transport; D012433:Ryanodine / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D002118:Calcium