title: Determination of histone acetylation status by chromatin immunoprecipitation.
abstract: Histone acetylation is the most studied posttranslation modification of nucleosomes. Understanding the mechanisms involved in global and promoter-specific histone acetylation will shed light on the control of transcriptional regulation. Chromatin immunoprecipitation is a powerful technique to study protein-DNA interactions in vivo. Proteins and DNA are cross-linked with formaldehyde, cells are lysed, and DNA is sheared by sonication. Protein-DNA complexes are immunoprecipitated with antibodies specific for total and acetylated histones and the relative occupancy of acetylated and total histones at selected loci is assessed by real-time PCR of the purified DNA.
authors: Department of Biological Sciences, St. John's University, Queens, NY, USA.
authors: Luciano
authors: L
authors: Galdieri
authors: John
authors: J
authors: Moon
authors: Ales
authors: A
authors: Vancura
mesh_terms: D000107:Acetylation; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D006657:Histones