title: [Construction and characterization of an ompR gene deletion mutant from Salmonella enteritidis].
abstract: OBJECTIVE
  To investigate the role of ompR gene from Salmonella enteritidis in biofilm formation and virulence.
  
  
  METHODS
  We constructed an ompR mutant of Salmonella enteritidis by suicide plasmid pGMB151. Biofilm forming ability of the mutant was detected by crystal violet assay and scanning electron micrography. Virulence of the mutant was determined by assay of adherence to and invasion of epithelial cells, and mouse challenge experiments.
  
  
  RESULTS
  The ompR mutant was confirmed by RT-PCR and the pattern of outer membrane protein. The mutant did not produce cellulose, curli, and biofilm, and showed similar adherence percentage to and invasion percentage of epithelial cells as wild type strain. In addition, intraperitoneal challenge of bacteria in BALB/c mice revealed that LD50 of the mutant strain was 10(6.67) CFU, while that of the wild type strain was less than 2 CFU.
  
  
  CONCLUSIONS
  These data indicate that the ompR gene is involved in both biofilm formation and virulence in Salmonella enteritidis.
authors: College of Veterinary Medicine, Animal Infectious Disease Laboratory, Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China. www1956@gmail.com
authors: Hongyan
authors: H
authors: Dong
authors: Daxin
authors: D
authors: Peng
authors: Xin'an
authors: X
authors: Jiao
authors: Xiaorong
authors: X
authors: Zhang
authors: Sujuan
authors: S
authors: Chen
authors: Yan
authors: Y
authors: Lu
authors: Shizhong
authors: S
authors: Geng
authors: Xiufan
authors: X
authors: Liu
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012477:Salmonella enteritidis / Q000235:genetics* / Q000472:pathogenicity; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics*; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins