title: One-copper laccase-related enzyme from Marasmius sp.: purification, characterization and bleaching of textile dyes.
abstract: In the culture filtrate of a Marasmius sp. strain isolated in Indonesia during a screening for fungi with the ability to decolorize textile dyes, two laccase-related enzymes (laccase-related enzyme I and II) were detected. Laccase-related enzyme I was purified to homogeneity by ion exchange and hydrophobic interaction chromatography. The native enzyme was shown to have a molecular mass of 53 kDa, an N-terminal amino acid sequence characteristically seen in laccases and an isoelectric point of pH 3.8. The enzyme accepts typical laccase substrates including 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), syringaldazine and guaiacol, but has no tyrosinase activity. The pH optimum is at pH 3.0 for ABTS and at 6.0 for syringaldazine and the enzyme is stable up to pH 10. The UV/vis spectrum of the laccase-related enzyme is non-typical for laccases and metal content analysis revealed that the enzyme contains only a single copper atom per enzyme molecule. This suggests that this enzyme could be related to the group of the so-called "white" laccases, however, no zinc or any other metal ion could be detected in this enzyme, suggesting that the enzyme is a unique laccase-related enzyme. Comparison of the bleaching activity of the whole fungus with that of the isolated laccase-related enzyme showed that this enzyme is the major bleaching enzyme produced by this Marasmius sp. strain and was able to bleach violet, red, orange and yellow dyes in addition to a number of blue dyes.
authors: Allgemeine Biochemie, Technische Universität Dresden, D-01062 Dresden, Germany.
authors: Julia
authors: J
authors: Schückel
authors: Anke
authors: A
authors: Matura
authors: Karl-Heinz
authors: KH
authors: van Pée
mesh_terms: D001673:Biodegradation, Environmental; D057886:Bleaching Agents / Q000378:metabolism*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007214:Indonesia; D007700:Kinetics; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055433:Marasmius / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D013696:Temperature; D013783:Textile Industry / Q000379:methods*; D013784:Textiles
chemical_list: D057886:Bleaching Agents; D004396:Coloring Agents; D003300:Copper; D042845:Laccase