title: [Efficiency of three commercial kits dedicated to DNA and RNA isolation from various clinical and forensic materials using the Janus automated workstation].
abstract: Isolation of genetic material is a crucial stage in molecular biology. Increasing needs for DNA analysis cause continuous improving of genetic material isolation methods toward higher accuracy and output. Automatization in molecular biology is widely seen, especially in clinical and forensic medicine. The objective of this research was optimization of methods for automatic nucleic acid isolation using the Janus automated workstation, Perkin Elmer. The efficiency and purity of isolated DNA was satisfactory. Despite numerous attempts at achieving automatic RNA isolation, we did not succeed in obtaining RNA working in other applications, such as RT-PCR or Real-Time PCR.
authors: Z Zakładu Technik Molekularnych Katedry Medycyny Sadowej Akademii Medycznej, im. Piastów Slaskich we Wrocławiu, Wrocław. malmal@forensic.am.wroc.pl
authors: Małgorzata
authors: M
authors: Małodobra
authors: Anna
authors: A
authors: Jonkisz
authors: Elzbieta
authors: E
authors: Kowalczyk
authors: Arleta
authors: A
authors: Lebioda
authors: Beata
authors: B
authors: Bartnik
authors: Barbara
authors: B
authors: Swiatek
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods; D018732:Forensic Anthropology / Q000379:methods*; D053803:Forensic Genetics; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D011044:Poland; D012313:RNA / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods
chemical_list: D011933:Reagent Kits, Diagnostic; D012313:RNA; D004247:DNA