title: Isolation of primary avian osteocytes.
abstract: Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
authors: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands. c.semeins@acta.nl
authors: Cor M
authors: CM
authors: Semeins
authors: Astrid D
authors: AD
authors: Bakker
authors: Jenneke
authors: J
authors: Klein-Nulend
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D002645:Chickens; D018189:Immunomagnetic Separation / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology; D010011:Osteocytes / Q000166:cytology* / Q000276:immunology; D012886:Skull / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal