title: Potato suberin induces differentiation and secondary metabolism in the genus Streptomyces.
abstract: Bacteria of the genus Streptomyces are soil microorganisms with a saprophytic life cycle. Previous studies have revealed that the phytopathogenic agent S. scabiei undergoes metabolic and morphological modifications in the presence of suberin, a complex plant polymer. This paper investigates morphological changes induced by the presence of potato suberin in five species of the genus Streptomyces, with emphasis on S. scabiei. Streptomyces scabiei, S. acidiscabies, S. avermitilis, S. coelicolor and S. melanosporofaciens were grown both in the presence and absence of suberin. In all species tested, the presence of the plant polymer induced the production of aerial hyphae and enhanced resistance to mechanical lysis. The presence of suberin in liquid minimal medium also induced the synthesis of typical secondary metabolites in S. scabiei and S. acidiscabies (thaxtomin A), S. coelicolor (actinorhodin) and S. melanosporofaciens (geldanamycin). In S. scabiei, the presence of suberin modified the fatty acid composition of the bacterial membrane, which translated into higher membrane fluidity. Moreover, suberin also induced thickening of the bacterial cell wall. The present data indicate that suberin hastens cellular differentiation and triggers the onset of secondary metabolism in the genus Streptomyces.
authors: Centre SÈVE, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.
authors: Sylvain
authors: S
authors: Lerat
authors: Martin
authors: M
authors: Forest
authors: Annie
authors: A
authors: Lauzier
authors: Gilles
authors: G
authors: Grondin
authors: Serge
authors: S
authors: Lacelle
authors: Carole
authors: C
authors: Beaulieu
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007211:Indoles / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D010879:Piperazines / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D011198:Solanum tuberosum / Q000382:microbiology*; D013302:Streptomyces / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D007211:Indoles; D008055:Lipids; D010879:Piperazines; C406691:thaxtomine A; C065875:suberin; C013390:actinorhodin; D000095622:Benzoisochromanequinones