title: Profiling of gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity.
abstract: Obesity-prone (OP) and obesity-resistant (OR) rats with different responses to development of obesity in spite of the same genetic background are useful animal models for searching for markers during the development of obesity. Here, we investigated whether plasma proteins of OP and OR rats may behave in a different way in males and females. We performed a comparative proteomic analysis using 2-DE combined with MALDI-TOF/MS on proteins from OP and OR male and female rats to discover gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity. A total of 29 proteins showing differential expression between the groups were identified by MALDI-TOF/MS and database searches. These proteins were classified into 4 groups according to their regulation patterns in response to diet and gender. 22 proteins showed significant differences between OP and OR rats in males and/or females (Group I, II, and III) and 7 proteins exhibited only a high fat diet (HFD)-responsive difference in male or female rats (Group IV). In conclusion, the proteins negatively (ITIH3, FGG, TUBB5, and ZAG) or positively (Hp, ITIH4, and RBP) correlated with obesity found in this study could be used for selection of new targets for gender specific-medical treatment of obesity.
authors: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk 712-714, Republic of Korea.
authors: Jung-Won
authors: JW
authors: Choi
authors: Hao
authors: H
authors: Liu
authors: Duk Kwon
authors: DK
authors: Choi
authors: Tae Seok
authors: TS
authors: Oh
authors: Rajib
authors: R
authors: Mukherjee
authors: Jong Won
authors: JW
authors: Yun
mesh_terms: D000821:Animal Feed; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D009765:Obesity / Q000209:etiology* / Q000235:genetics*; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; D010455:Peptides