title: A starter kit for point-localization super-resolution imaging.
abstract: Super-resolution fluorescence imaging can be achieved through the localization of single molecules. By using suitable dyes, optical configurations, and software, it is possible to study a wide variety of biological systems. Here, we summarize the different approaches to labeling proteins. We review proven imaging modalities, and the features of freely available software. Finally, we give an overview of some biological applications. We conclude by synthesizing these different technical aspects into recommendations for standards that the field might apply to ensure quality of images and comparability of algorithms and dyes.
authors: Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. suliana.manley@epfl.ch
authors: Suliana
authors: S
authors: Manley
authors: Julia
authors: J
authors: Gunzenhäuser
authors: Nicolas
authors: N
authors: Olivier
mesh_terms: D000465:Algorithms; D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000032:analysis / Q000138:chemical synthesis*; D055668:Photochemical Processes; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012984:Software
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D011506:Proteins