title: The antidepressant-like effect of Hedyosmum brasiliense and its sesquiterpene lactone, podoandin in mice: evidence for the involvement of adrenergic, dopaminergic and serotonergic systems.
abstract: We have recently shown that the ethanol extract of the leaves of Hedyosmum brasiliense exhibits an antidepressant-like effect in the tail suspension and forced swimming tests in mice. The present study investigates the mechanisms involved in the antidepressant-like effect of H. brasiliense extract, together with the antidepressant potential of podoandin, an isolated sesquiterpenoid. H. brasiliense (50mg/kg, i.p.) and podoandin (10mg/kg, i.p.) decreased the immobility time in the forced swimming test, without any accompanying changes in ambulation in the open-field test. The anti-immobility effect of the H. brasiliense extract was prevented by pre-treating the mice with ondansetron, NAN 190, pindolol, prazosin, yohimbine, haloperidol, SCH23390, and sulpiride. On the other hand, pre-treating the mice with: p-chlorophenylalanine (4 consecutive days), ketanserin, naloxone, naltrindole, bicuculline, phaclofen, or l-arginine did not block the antidepressant-like effect of H. brasiliense. In addition, pre-treatment of the animals with methylene blue, NG-nitro-l-arginine or 7-nitroindazole, at subeffective doses, did not cause a synergistic effect with H. brasiliense extract at an effective dose in the forced swimming test. The anti-immobility effect of podoandin was also prevented by pre-treating the mice with NAN-190, ondansetron, prazosin, yohimbine, sulpiride and haloperidol. The results indicate that the antidepressant-like effect of H. brasiliense (and podoandin) is dependent on the serotonergic, noradrenergic and dopaminergic systems, but not on the GABAergic, opioid and oxidonitrergic systems.
authors: Centro de Ciências da Saúde, CCS - Mestrado em Ciências Farmacêuticas, UNIVALI, Itajaí, SC, Brazil.
authors: Ana Elisa
authors: AE
authors: Gonçalves
authors: Cristiani
authors: C
authors: Bürger
authors: Solomon K S
authors: SK
authors: Amoah
authors: Rogério
authors: R
authors: Tolardo
authors: Maique W
authors: MW
authors: Biavatti
authors: Márcia M
authors: MM
authors: de Souza
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D000701:Analgesics, Opioid / Q000378:metabolism; D000818:Animals; D000928:Antidepressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001120:Arginine / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D003508:Cycloheptanes / Q000302:isolation & purification / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism; D004347:Drug Interactions; D004837:Epinephrine / Q000378:metabolism; D029624:Ferns / Q000737:chemistry*; D005473:Fluoxetine / Q000494:pharmacology; D007783:Lactones / Q000302:isolation & purification / Q000494:pharmacology*; D008124:Locomotion / Q000187:drug effects; D008297:Male; D051379:Mice; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D013550:Swimming
chemical_list: D000701:Analgesics, Opioid; D000928:Antidepressive Agents; D003508:Cycloheptanes; D007783:Lactones; D018377:Neurotransmitter Agents; D010936:Plant Extracts; D012717:Sesquiterpenes; D005473:Fluoxetine; C416071:podoandin; D009569:Nitric Oxide; D012701:Serotonin; D001120:Arginine; D006152:Cyclic GMP; D015107:4-Butyrolactone; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine