title: Age-related changes in myosin-V myenteric neurons, CGRP and VIP immunoreactivity in the ileum of rats supplemented with ascorbic acid.
abstract: We examined the effects of ascorbic acid supplementation on myosin-V, calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP) immunoractivities in the myenteric neurons in aging rats. Male rats were divided into groups: young 90-day-old rats (E90), 345-day-old control rats (E345), 428-day-old control rats (E428), 90- to 345-day-old rats treated with ascorbic acid (1 g/L) (EA345), and 90- to 428-day-old rats treated with ascorbic acid (1g/L) (EA428). The quantitative results showed that aging reduced the number of myosin-V-immunoreactive neurons compared with young animals (E90). Ascorbic acid supplementation in the EA345 and EA428 groups increased the average area of myosin-V neurons by 24.6% and 24.1% compared with the E345 and E428 groups, respectively. When all groups were compared, we observed significant differences for the CGRP- and VIP-immunoractive varicosities of nerve fibers from myenteric neurons. Ascorbic acid supplementation had a neurotrophic effect on all neurons studied, suggesting a neuroprotective role.
authors: Department of Morphological Sciences, Statal University of Maringá, PR, Brazil.
authors: A P T
authors: AP
authors: Veit
authors: J N
authors: JN
authors: Zanoni
mesh_terms: D000367:Age Factors; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D019587:Dietary Supplements; D007082:Ileum / Q000294:innervation*; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D024701:Myosin Type V / Q000378:metabolism*; D009412:Nerve Fibers / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D018696:Neuroprotective Agents; D014660:Vasoactive Intestinal Peptide; D024701:Myosin Type V; D015740:Calcitonin Gene-Related Peptide; D001205:Ascorbic Acid