title: Anti-inflammatory activities of aqueous extract of Mesona procumbens in experimental mice.
abstract: BACKGROUND
  Mesona procumbens is consumed as a herbal drink and jelly-type dessert in Taiwan. The aim of this study was to determine the mechanism of anti-inflammatory activities of the aqueous extract of M. procumbens (AMP) using the λ-carrageenin (Carr)-induced mouse paw oedema model. The fingerprint chromatogram of AMP was obtained by high-performance liquid chromatography (HPLC) analysis. To investigate the anti-inflammatory mechanism of AMP, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the level of malondialdehyde (MDA) in paw oedema were monitored. Serum nitric oxide (NO), tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were also evaluated.
  
  
  RESULTS
  The fingerprint chromatogram from HPLC indicated that AMP contained protocatechuic acid, chlorogenic acid, vanillic acid and caffeic acid. In the anti-inflammatory test, AMP decreased paw oedema after Carr administration and increased the CAT, SOD and GPx activities and decreased the MDA level in paw oedema at 5 h after Carr injection. AMP also affected the serum NO, TNF-α and IL-1β levels at 5 h after Carr injection. Western blotting revealed that AMP decreased the expression of Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2).
  
  
  CONCLUSIONS
  Mesona procumbens has the potential to provide a therapeutic approach to inflammation-associated disorders.
authors: School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. gjhuang@mail.cmu.edu.tw
authors: Guan-Jhong
authors: GJ
authors: Huang
authors: Jung-Chun
authors: JC
authors: Liao
authors: Chuan-Sung
authors: CS
authors: Chiu
authors: Shyh-Shyun
authors: SS
authors: Huang
authors: Tsung-Hui
authors: TH
authors: Lin
authors: Jeng-Shyan
authors: JS
authors: Deng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D015153:Blotting, Western; D002109:Caffeic Acids / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan; D002374:Catalase / Q000378:metabolism; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D052246:Cyclooxygenase 2 Inhibitors / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D004487:Edema / Q000188:drug therapy* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D062385:Hydroxybenzoates / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use; D018836:Inflammation Mediators / Q000378:metabolism*; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D052246:Cyclooxygenase 2 Inhibitors; D062385:Hydroxybenzoates; D018836:Inflammation Mediators; D010936:Plant Extracts; D002726:Chlorogenic Acid; C009091:protocatechuic acid; D008315:Malondialdehyde; D002351:Carrageenan; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; C040048:caffeic acid