title: Repertoire of endothelial progenitor cells mobilized by femoral artery ligation: a nonhuman primate study.
abstract: To determine in the baboon model the identities and functional characteristics of endothelial progenitor cells (EPCs) mobilized in response to artery ligation, we collected peripheral blood mononuclear cells (PBMNCs) before and 3 days after a segment of femoral artery was removed. Our goal was to find EPC subpopulations with highly regenerative capacity. We identified 12 subpopulations of putative EPCs that were altered >1.75-fold; two subpopulations (CD146+/CD54-/CD45- at 6.63-fold, and CD146+/UEA-1-/CD45- at 12.21-fold) were dramatically elevated. To investigate the regenerative capacity of putative EPCs, we devised a new assay that maximally resembled their in vivo scenario, we purified CD34+ and CD146+ cells and co-cultured them with basal and mobilized PBMNCs; both cell types took up Dil-LDL, but purified CD146+ cells exhibited accelerated differentiation by increasing expression of CD31 and CD144, and by exhibiting more active cord-like structure formation by comparison to the CD34+ subpopulation in a co-culture with mobilized PBMNCs. We demonstrate that ischaemia due to vascular ligation mobilizes multiple types of cells with distinct roles. Baboon CD146+ cells exhibit higher reparative capacity than CD34+ cells, and thus are a potential source for therapeutic application.
authors: Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX 78245-0549, USA. qshi@txbiomedgenetics.org
authors: Qiang
authors: Q
authors: Shi
authors: Laura A
authors: LA
authors: Cox
authors: Vida
authors: V
authors: Hodara
authors: Xing Li
authors: XL
authors: Wang
authors: John L
authors: JL
authors: VandeBerg
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000302:isolation & purification; D051929:CD146 Antigen / Q000302:isolation & purification; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005263:Femoral Artery / Q000378:metabolism / Q000601:surgery*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling / Q000379:methods; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008026:Ligation; D008297:Male; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010215:Papio; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D051929:CD146 Antigen