title: Multifrequency impedance measurement technique for wireless characterization of microbiological cell cultures.
abstract: An impedance measurement system with probe signal frequencies up to 50 kHz with AC-probe voltages below 30 mV rms was integrated for wireless and battery-free monitoring of microbiological cell cultures. The here presented modular design and the use of state-of-the-art components greatly eases adoptions to a wide range of biotechnological applications without the need of bulky LCR-meters or potentiostats. The device had a power consumption of less than 2.5 mA at a 3.3 V single power supply and worked trouble-free within the humid environment of a cell culture incubator. Measurements on lumped RC-elements showed an error of less than 1% for absolute values and less than 1° regarding the phase of the complex impedance. The performance of sensor devices with interdigitated electrode structures for the measurement of adherent cell cultures was tested in the presence of phosphate-buffered saline solution in the humid atmosphere of an incubator for biological cell cultures.
authors: Nano-Systems, AIT Austrian Institute of Technology GmbH, 1220 Wien, Austria.
authors: J
authors: J
authors: Wissenwasser
authors: M J
authors: MJ
authors: Vellekoop
authors: W
authors: W
authors: Kapferer
authors: G
authors: G
authors: Lepperdinger
authors: R
authors: R
authors: Heer
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D008828:Microbiological Techniques / Q000295:instrumentation*; D059015:Wireless Technology / Q000295:instrumentation*