title: Upregulation of microRNA-210 regulates renal angiogenesis mediated by activation of VEGF signaling pathway under ischemia/perfusion injury in vivo and in vitro.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are endogenous, non-coding, small RNAs that regulate gene expression and function, but little is known about regulation of miRNAs in the kidneys under normal or pathologic conditions. Here, we sought to investigate the potential involvement of miRNAs in renal ischemia/reperfusion (I/R) injury and angiogenesis and to define some of the miRNAs possibly associated with renal angiogenesis.
  
  
  RESULTS
  Male Balb/c mice were subjected to a standard renal I/R. CD31 immunostaining indicated a significant increase of microvessels in the ischemic region. VEGF and VEGFR2 expression were increased in renal I/R at both the mRNA and protein levels which were detected by qRT-PCR and Western blot, respectively. More importantly, 76 microRNAs exhibited more than 2-fold changes using Agilent microRNA microarray, which contains downregulation of 40 miRNAs and upregulation of 36 miRNAs. Upregulation of miR-210 was confirmed by qRT-PCR with prominent changes at 4 and 24 h after reperfusion. Furthermore, overexpression of miR-210 in HUVEC-12 cells enhances VEGF and VEGFR2 expression and promotes angiogenesis on Matrigel in vitro.
  
  
  CONCLUSIONS
  These findings suggest miR-210 may be involved in targeting the VEGF signaling pathway to regulate angiogenesis after renal I/R injury, which provides novel insights into the angiogenesis mechanism of renal I/R injury.
authors: Institute of Urology, Nanchang University, Nanchang, China.
authors: Fen
authors: F
authors: Liu
authors: Yuan-Lei
authors: YL
authors: Lou
authors: Jue
authors: J
authors: Wu
authors: Qiong-Fang
authors: QF
authors: Ruan
authors: An
authors: A
authors: Xie
authors: Fei
authors: F
authors: Guo
authors: Su-Ping
authors: SP
authors: Cui
authors: Zhi-Feng
authors: ZF
authors: Deng
authors: Yang
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000096:biosynthesis*; D009389:Neovascularization, Pathologic / Q000378:metabolism* / Q000503:physiopathology; D011897:Random Allocation; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D015398:Signal Transduction / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism* / Q000502:physiology
chemical_list: C549325:MIRN210 microRNA, mouse; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse