title: Polyphenol contents and antioxidant activity of Brassica nigra (L.) Koch. leaf extract.
abstract: Profound research has been done on the medicinal value of Brassica nigra (BN) seeds, and the leaves of the plant have been investigated in this study. The methanol extracts of the leaves were subjected to several in vitro studies. The antioxidant activity of methanol extract was demonstrated with a wide range of concentration, 10-500 µg mL(-1), and the antioxidant activity increased with the increase in concentration. Total phenol content was found to be 171.73 ± 5.043 gallic acid equivalents and the total flavonoid content 7.45 ± 0.0945 quercetin equivalents. Further quantification and identification of the compounds were done by HPTLC and GC-MS analyses. The predominant phenolic compounds determined by HPTLC were gallic acid, followed by quercetin, ferulic acid, caffeic acid and rutin. The free radical quenching property of BN leaf extract suggests the presence of bioactive natural compounds.
authors: PG & Research Department of Biochemistry, M.G.R. College, Hosur 635 109, India.
authors: R
authors: R
authors: Rajamurugan
authors: N
authors: N
authors: Selvaganabathy
authors: S
authors: S
authors: Kumaravel
authors: Ch
authors: Ch
authors: Ramamurthy
authors: V
authors: V
authors: Sujatha
authors: C
authors: C
authors: Thirunavukkarasu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002109:Caffeic Acids / Q000032:analysis; D003373:Coumaric Acids / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry; D005707:Gallic Acid / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis*; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D059808:Polyphenols; D012431:Rutin; D005707:Gallic Acid; D011794:Quercetin; C004999:ferulic acid; C040048:caffeic acid