title: PRSS1 intron mutations in patients with pancreatic cancer and chronic             pancreatitis.
abstract: Genetic risk factors of chronic pancreatitis (CP) have been identified and a number of studies have found that CP can lead to pancreatic cancer. Therefore, the detection of pancreatitis-associated gene mutations can aid the pancreatic cancer diagnostic process. Mutations in three genes, the cationic trypsinogen (PRSS1) gene, the cystic fibrosis transmembrane conductance regulator (CFTR) gene and the pancreatic secretory trypsin inhibitor (SPINK1) gene, have been identified as risk factors for CP. The aim of this study was to describe specific novel mutations in the intron of the PRSS1 gene in patients with pancreatic cancer and CP. A total of 65 unrelated patients with pancreatic cancer and 29 with CP were reviewed. Mutations and polymorphisms of the PRSS1 gene were analyzed by direct sequencing. Information regarding clinical data and smoking exposure was collected by personal interviews using a structured questionnaire. IVS 3+36 A>G mutation in the PRSS1 gene was found in 2 cases with pancreatic cancer, and these 2 patients were classified as never-smokers. IVS 3+127 T>A and IVS 3+157 G>C double mutations were identified in one patient with CP. All patients were found to have serum trypsin levels lower than that of the normal controls. Therefore, the PRSS1 gene mutation may be a special common cause of pancreatic cancer and CP.
authors: Department of Pathology, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, PR China.
authors: Feng
authors: F
authors: Gao
authors: Qi-Cai
authors: QC
authors: Liu
authors: Sheng
authors: S
authors: Zhang
authors: Ze-Hao
authors: ZH
authors: Zhuang
authors: Cai-Zhu
authors: CZ
authors: Lin
authors: Xin-Hua
authors: XH
authors: Lin
mesh_terms: D015331:Cohort Studies; D005260:Female; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*; D050500:Pancreatitis, Chronic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D012907:Smoking; D014357:Trypsin / Q000097:blood / Q000235:genetics*
chemical_list: C489303:PRSS1 protein, human; D014357:Trypsin