title: Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases.
abstract: In biomass degradation using simultaneous saccharification and fermentation (SSF), there is a need for efficient biomass degrading enzymes that can work at lower temperatures suitable for yeast fermentation. As xylan is an important lignocellulosic biomass constituent, this study aimed at investigating the possible differences in xylan breakdown potential of endoxylanases using eight different endoxylanases at conditions relevant for SSF. Both solubilising and degrading capacities of the endoxylanases were investigated using water-insoluble and water-soluble oat spelt xylan as model substrates for biomass xylan. Results showed that selecting for combinations of endoxylanases that are efficient at solubilising xylan on the one hand and degrading it to large extent on the other hand, coupled to high specific activities, seems the best option for complete xylan breakdown in lignocellulosic biomass conversion using SSF.
authors: Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20 bus 2463, B-3001 Leuven, Belgium.
authors: Sven
authors: S
authors: Cuyvers
authors: Emmie
authors: E
authors: Dornez
authors: Karolien
authors: K
authors: Moers
authors: Annick
authors: A
authors: Pollet
authors: Jan A
authors: JA
authors: Delcour
authors: Christophe M
authors: CM
authors: Courtin
mesh_terms: D018554:Avena; D001426:Bacterial Proteins / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D018533:Biomass; D043364:Endo-1,4-beta Xylanases / Q000145:classification / Q000378:metabolism*; D005285:Fermentation; D019649:Food Industry / Q000379:methods*; D005516:Food Microbiology; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010940:Plant Proteins / Q000378:metabolism; D012995:Solubility; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D010940:Plant Proteins; D014990:Xylans; D014994:Xylose; D043364:Endo-1,4-beta Xylanases