title: Combined effect of cytokine gene polymorphisms on end-stage knee osteoarthritis from Chinese Han population.
abstract: The mechanism of osteoarthritis (OA) is not well understood. Cytokines have been implicated in the episode, and there is increasing evidence that the host's cytokine response is genetically determined. We determined the predictive value of IL-634G./C, ICAM-1 469 K/E and IL-10-1082A/G, -819T/C and -592A/C gene polymorphisms on knee OA. The study included 1007 patients with end-stage knee OA and 910 healthy controls. Genomic DNA was prepared from peripheral blood leukocytes. Genotypes and allele frequencies were determined using restriction fragment length polymorphism analysis of polymerase chain reaction products. No significant difference in the IL-10 promoter allele or haplotype frequencies between end-stage knee OA and controls was found. Patients with end-stage knee OA showed a significantly higher prevalence of IL-6-634G/ICAM-1 469E carrier than that in controls (P = 0.017). Results indicate that IL-6-634G/ICAM-1 469E carrier could be associated with increased susceptibility to end-stage knee OA.
authors: Department of Joint Surgery, Associated Hospital of Qingdao University Medical College, 16 Jiangsu Road, Qingdao, Shandong Province, 266003, People's Republic of China. qyfylcy@163.com
authors: Chengyu
authors: C
authors: Lv
authors: Xiaoyan
authors: X
authors: Xu
authors: Jinqing
authors: J
authors: Wang
authors: Zhongyi
authors: Z
authors: Zhang
authors: Dianliang
authors: D
authors: Zhang
authors: Chuanyou
authors: C
authors: Guo
authors: Chengxin
authors: C
authors: Geng
authors: Yunbo
authors: Y
authors: Sun
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10