title: Mouse and human mesoangioblasts: isolation and characterization from adult skeletal muscles.
abstract: Mesoangioblasts (MABs) are mesoderm-derived stem cells, associated with small vessels and originally described in the mouse embryonic dorsal aorta. Similar though not identical cells have been later identified and characterized from postnatal small vessels of skeletal muscle and heart. They have in common the expression of pericyte markers, the anatomical location, the ability to self-renew in culture, and to differentiate into various types of mesodermal lineages upon proper culture conditions. Currently, the developmental origin of MABs and the relationship with other muscle stem cells are not understood in detail and are the subject of active research. This chapter provides an outline of the latest techniques for isolation and characterization of adult MABs from human and mouse skeletal muscles.
authors: Translational Cardiomyology, Stem Cell Research Institute, Catholic University of Leuven, Leuven, Belgium.
authors: Mattia
authors: M
authors: Quattrocelli
authors: Giacomo
authors: G
authors: Palazzolo
authors: Ilaria
authors: I
authors: Perini
authors: Stefania
authors: S
authors: Crippa
authors: Marco
authors: M
authors: Cassano
authors: Maurilio
authors: M
authors: Sampaolesi
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D018920:Coculture Techniques; D015925:Cryopreservation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation