title: Oxidative DNA damage correlates with carotid artery atherosclerosis in hemodialysis patients.
abstract: Oxidative stress is accepted as a nonclassical cardiovascular risk factor in chronic renal failure patients. The aim of this study was to evaluate the relation between oxidative DNA damage (8-hydroxy-2'-deoxyguanosine/deoxyguanosine [8-OHdG/dG] ratio), oxidative stress biomarkers, antioxidant enzymes, and carotid artery intima-media thickness (CIMT) in hemodialysis (HD) patients. Forty chronic HD patients without known atherosclerotic disease and 48 age- and sex-matched healthy individuals were included in the study. Plasma malondialdehyde (MDA) levels and 8-OHdG/dG ratio were determined as oxidative stress markers. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured as antioxidants. CIMT was assessed by carotid artery ultrasonography. 8-OHdG/dG ratios and MDA levels were higher; SOD and GPx activities were lower in HD patients compared to controls. HD patients had significantly higher CIMT compared to controls (0.61 ± 0.08 vs. 0.42 ± 0.05, p < 0.001). There was a significant positive correlation between CIMT and 8-OHdG/dG ratio (r = 0.57, p < 0.01) and MDA levels (r = 0.41, p < 0.01), while there was a significant negative correlation between CIMT and SOD (r = -0.47, p < 0.01) and GPx levels (r = -0.62, p < 0.01). It is firstly demonstrated that CIMT is positively correlated with oxidative DNA damage in HD patients without known atherosclerotic disease.
authors: Department of Nephrology, Van Yuksek Ihtisas Hospital, Van, Turkey. elifari@gmail.com
authors: Elif
authors: E
authors: Ari
authors: Yuksel
authors: Y
authors: Kaya
authors: Halit
authors: H
authors: Demir
authors: Aysegul
authors: A
authors: Cebi
authors: Hamit Hakan
authors: HH
authors: Alp
authors: Ebubekir
authors: E
authors: Bakan
authors: Dolunay
authors: D
authors: Odabasi
authors: Sıddık
authors: S
authors: Keskin
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D050197:Atherosclerosis / Q000097:blood* / Q000209:etiology; D015415:Biomarkers / Q000378:metabolism; D002340:Carotid Artery Diseases / Q000097:blood* / Q000209:etiology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine