title: Osteogenic capability of autologous rabbit adipose-derived stromal cells in repairing calvarial defects.
abstract: OBJECTIVE
  To evaluate the in vitro and in vivo osteogenic capability of adipose-derived stromal cells (ASCs).
  
  
  METHODS
  ASCs were isolated from New Zealand white rabbits and determined by alkaline phosphatase (ALP) staining, von Kossa staining and alizarin red staining. Some specific markers of osteogenic differentiation, including ALP, osteocalcin (OCN), osteopontin (OPN) were examined by reverse transcription-polymerase chain reaction (RT-PCR). In vivo, demineralized bone matrix (DBM)-ASCs composites were implanted into the rabbit calvarial defects created at each side of the longitudinal midline. After 6 weeks, histologic properties of the transplants were analyzed.
  
  
  RESULTS
  ASCs were successfully induced into osteogenesis. ALP staining, von Kossa staining and alizarin red staining showed positive results. The expressions of ALP, OCN and OPN were detected in ASCs after cultivation in osteogenic medium. Extensive new bone was observed in the defects transplanted with DBM-ASCs composites.
  
  
  CONCLUSIONS
  ASCs have the potential to differentiate into osteogenic lineage and DBM-ASCs constructs are a promising method for regeneration in bone defects.
authors: Department of Orthopaedics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000.
authors: Shao-Wen
authors: SW
authors: Cheng
authors: Zhong-Qin
authors: ZQ
authors: Lin
authors: Wei
authors: W
authors: Wang
authors: Wei
authors: W
authors: Zhang
authors: Dong-Quan
authors: DQ
authors: Kou
authors: Xiao-Zhou
authors: XZ
authors: Ying
authors: Qing-Yu
authors: QY
authors: Chen
authors: Yue
authors: Y
authors: Shen
authors: Xiao-Jie
authors: XJ
authors: Cheng
authors: Lei
authors: L
authors: Peng
authors: Chuan-Zhu
authors: CZ
authors: Lv
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D010012:Osteogenesis; D011817:Rabbits; D017154:Stromal Cells