title: Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation.
abstract: OBJECTIVE
  To investigate the effect of alendronate on the expression of Id genes in osteoblast differentiation.
  
  
  METHODS
  C2C12 cells were treated with alendronate for various concentrations and time periods. For evaluation of alendronate-induced osteoblast differentiation in C2C12 cells, alkaline phosphatase (ALP) activity was measured. The expression of osteoblast differentiation markers such as ALP, type-1 collagen (Col 1), and osteocalcin (OCN), and the expression of Id-1 and Id-2 were measured by RT-PCR. In order to understand the mechanism underlying the regulation of Id genes, the promoter region of the Id-1 gene was identified. Database analysis of the promoter region for Id-1 using known consensus sequences identified several putative response elements, including CCAAT/enhancer-binding protein beta (C/EBPβ).
  
  
  RESULTS
  Alendronate treatment significantly increased not only ALP activity but also the expression of ALP, Col 1, and OCN, Id-1 and Id-2. C/EBPβ and alendronate cooperatively increased the promoter activity and expression of Id-1.
  
  
  CONCLUSIONS
  These results suggest that C/EBPβ-mediated Id-1 transcriptional activation may regulate alendronate-induced osteoblast differentiation of C2C12 cells.
authors: Department of Obstetrics and Gynecology, College of Medicine, Kosin University, Busan, Republic of Korea.
authors: Ae Ra
authors: AR
authors: Kang
authors: Young Rim
authors: YR
authors: Oh
authors: Heung Yeol
authors: HY
authors: Kim
authors: Min Jung
authors: MJ
authors: Park
authors: Bo Sun
authors: BS
authors: Joo
authors: Won Jun
authors: WJ
authors: Choi
authors: Ji Young
authors: JY
authors: Lee
authors: Min Hyung
authors: MH
authors: Jung
authors: Yong Il
authors: YI
authors: Ji
authors: Jong Soon
authors: JS
authors: Choi
mesh_terms: D019386:Alendronate / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects*; D015854:Up-Regulation
chemical_list: D050071:Bone Density Conservation Agents; D051796:Inhibitor of Differentiation Proteins; D000469:Alkaline Phosphatase; D019386:Alendronate