title: Systematic analyses of genes associated with radiosensitizing effect by celecoxib, a specific cyclooxygenase-2 inhibitor.
abstract: To investigate genes regulated by COX-2 or a COX-2 specific inhibitor, celecoxib, in irradiated cancer cells, we analyzed changes in gene expression using complementary DNA microarray following celecoxib or combined celecoxib and ionizing radiation (IR) treatment in a stable COX-2 knockdown A549 (AS) and a mock cell line (AN). Thirty-six genes were differentially expressed by COX-2 knockdown. Celecoxib changed the expressions of 40 and 69 genes in AN and AS cells, respectively. Twenty-seven genes were synchronously regulated by COX-2 and celecoxib. Among these, celecoxib regulated ras homolog gene family B and mitosin protein expression in a COX-2 dependent manner, especially in irradiated cells. In addition, we identified 11 genes that changed by more than 1.5 times the expected additive values after celecoxib and IR treatment. The current study may provide evidence that COX-2 or celecoxib regulates various intracellular functions in addition to their enzymatic activity regulation. We also identified candidate molecules that may be responsible for COX-2-dependent radiosensitization by celecoxib.
authors: Department of Radiation Oncology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul, Korea.
authors: Young-Mee
authors: YM
authors: Kim
authors: You Keun
authors: YK
authors: Shin
authors: Hyun Jung
authors: HJ
authors: Jun
authors: Sun Young
authors: SY
authors: Rha
authors: Hongryull
authors: H
authors: Pyo
mesh_terms: D000068579:Celecoxib; D045744:Cell Line, Tumor; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000172:deficiency / Q000235:genetics; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000528:radiation effects*; D055785:Gene Knockdown Techniques; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000187:drug effects* / Q000235:genetics*; D013449:Sulfonamides / Q000494:pharmacology*; D020743:rhoB GTP-Binding Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D052246:Cyclooxygenase 2 Inhibitors; D008840:Microfilament Proteins; D011720:Pyrazoles; D034741:RNA, Small Interfering; D013449:Sulfonamides; C094772:centromere protein F; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D020743:rhoB GTP-Binding Protein; D000068579:Celecoxib