title: Laser capture microdissection with genome-wide expression profiling displayed gene expression signatures in endometrioid endometrial cancer.
abstract: This research determined genes contributing to the pathogenesis of endometrioid endometrial cancer (EEC). Eight pairs of microdissected EEC samples matched with normal glandular epithelium were analyzed using microarray. Unsupervised analysis identified 162 transcripts (58 up- and 104 down-regulated) that were differentially expressed (p < .01, fold change ≥ 1.5) between both groups. Quantitative real-time polymerase chain reaction (qPCR) validated the genes of interest: SLC7A5, SATB1, H19, and ZAK (p < .05). Pathway analysis revealed genes involved in acid amino transport, translation, and chromatin remodeling (p < .05). Laser capture microdissection (LCM) followed by microarray enabled precise assessment of homogeneous cell population and identified putative genes for endometrial carcinogenesis.
authors: UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Cheras 56000, Kuala Lumpur, Malaysia. norfilza@medic.ukm.my
authors: Norfilza Mohd
authors: NM
authors: Mokhtar
authors: Nurul Hanis
authors: NH
authors: Ramzi
authors: Wong
authors: W
authors: Yin-Ling
authors: Isa Mohamed
authors: IM
authors: Rose
authors: Ahmad Zailani
authors: AZ
authors: Hatta Mohd Dali
authors: Rahman
authors: R
authors: Jamal
mesh_terms: D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D015994:Incidence; D061346:Laser Capture Microdissection / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015854:Up-Regulation