title: Ultrasonic synthetic technique to manufacture a pHEMA nanopolymeric-based vaccine against the H6N2 avian influenza virus: a preliminary investigation.
abstract: This preliminary study investigated the use of poly (2-hydroxyethyl methacrylate) (pHEMA) nanoparticles for the delivery of the deoxyribonucleic acid (DNA) vaccine pCAG-HAk, which expresses the full length hemagglutinin (HA) gene of the avian influenza A/Eurasian coot/Western Australian/2727/1979 (H6N2) virus with a Kozak sequence which is in the form of a pCAGGS vector. The loaded and unloaded nanoparticles were characterized using field-emission scanning electron microscopy. Further characterizations of the nanoparticles were made using atomic force microscopy and dynamic light scattering, which was used to investigate particle size distributions. This preliminary study suggests that using 100 μg of pHEMA nanoparticles as a nanocarrier/adjuvant produced a reduction in virus shedding and improved the immune response to the DNA vaccine pCAG-HAk.
authors: Murdoch Applied Nanotechnology Research Group, Murdoch University, Murdoch, WA, Australia. g.poinern@murdoch.edu.au
authors: Gérrard Eddy Jai
authors: GE
authors: Poinern
authors: Xuan Thi
authors: XT
authors: Le
authors: Songhua
authors: S
authors: Shan
authors: Trevor
authors: T
authors: Ellis
authors: Stan
authors: S
authors: Fenwick
authors: John
authors: J
authors: Edwards
authors: Derek
authors: D
authors: Fawcett
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D000431:Ethanol; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D005585:Influenza in Birds / Q000517:prevention & control; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D019444:Vaccines, DNA / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D017201:Virus Shedding
chemical_list: D004337:Drug Carriers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D019444:Vaccines, DNA; D011102:Polyhydroxyethyl Methacrylate; D000431:Ethanol; D004247:DNA