title: Gene expression in livers of BALB/C and C57BL/6J mice fed a high-fat diet.
abstract: We previously demonstrated that high-fat diet (HFD)-induced hepatic lipid accumulation is more severe in BALB/c mice than in C57BL/6J (B6) mice. To understand the changes in liver metabolism, we studied blood chemistry, gene expression, and histopathological changes of the liver in nine-week HFD-fed BALB/c and B6 mice and one- or four-week HFD-fed BALB/c mice. Serum total cholesterol and triglyceride levels were significantly increased in all HFD-fed groups, and one- and four-week HFD-fed BALB/c groups, respectively. Histopathology revealed that vacuolation of hepatocytes was severe in nine-week HFD-fed BALB/c mice, although it was less severe in the other groups. Microarray analysis of mRNA expression of nine-week HFD-fed BALB/c mice showed up-regulation of genes involved in fatty acid uptake and biosynthesis, such as Cd36, Acaca, Acly, and Fasn. Some changes were observed in the one- and four-week HFD-fed BALB/c groups and the nine-week HFD-fed B6 group, however these changes in mRNA expression were not so marked. In conclusion, the fatty accumulation observed in BALB/c mice may be caused, at least in part, by up-regulation of fatty acid uptake and biosynthesis. Cd36, Acaca, Acly and Fasn may be involved in these metabolic processes.
authors: Safety Research Laboratory, Research Division, Mitsubishi Tanabe Pharma Corporation, Kisarazu, Chiba, Japan. Nishikawa.Satomi@mk.mt-pharma.co.jp
authors: Satomi
authors: S
authors: Nishikawa
authors: Jiro
authors: J
authors: Sugimoto
authors: Miyoko
authors: M
authors: Okada
authors: Tetsuya
authors: T
authors: Sakairi
authors: Shiro
authors: S
authors: Takagi
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size / Q000187:drug effects; D014280:Triglycerides / Q000097:blood; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D014280:Triglycerides; D002784:Cholesterol