title: Analysis of protein networks in resting and collagen receptor (GPVI)-stimulated platelet sub-proteomes.
abstract: Proteomics approaches have made important contributions to the characterisation of platelet regulatory mechanisms. A common problem encountered with this method, however, is the masking of low-abundance (e.g. signalling) proteins in complex mixtures by highly abundant proteins. In this study, subcellular fractionation of washed human platelets either inactivated or stimulated with the glycoprotein (GP) VI collagen receptor agonist, collagen-related peptide, reduced the complexity of the platelet proteome. The majority of proteins identified by tandem mass spectrometry are involved in signalling. The effect of GPVI stimulation on levels of specific proteins in subcellular compartments was compared and analysed using in silico quantification, and protein associations were predicted using STRING (the search tool for recurring instances of neighbouring genes/proteins). Interestingly, we observed that some proteins that were previously unidentified in platelets including teneurin-1 and Van Gogh-like protein 1, translocated to the membrane upon GPVI stimulation. Newly identified proteins may be involved in GPVI signalling nodes of importance for haemostasis and thrombosis.
authors: Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, Berkshire, UK. b.wright@reading.ac.uk
authors: Bernice
authors: B
authors: Wright
authors: Ronald G
authors: RG
authors: Stanley
authors: William J
authors: WJ
authors: Kaiser
authors: Davinia J
authors: DJ
authors: Mills
authors: Jonathan M
authors: JM
authors: Gibbins
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D006801:Humans; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D010980:Platelet Membrane Glycoproteins; D020543:Proteome; C098341:platelet membrane glycoprotein VI; D003094:Collagen