title: Different effects of the TAR structure on HIV-1 and HIV-2 genomic RNA translation.
abstract: The 5'-untranslated region (5'-UTR) of the genomic RNA of human immunodeficiency viruses type-1 (HIV-1) and type-2 (HIV-2) is composed of highly structured RNA motifs essential for viral replication that are expected to interfere with Gag and Gag-Pol translation. Here, we have analyzed and compared the properties by which the viral 5'-UTR drives translation from the genomic RNA of both human immunodeficiency viruses. Our results showed that translation from the HIV-2 gRNA was very poor compared to that of HIV-1. This was rather due to the intrinsic structural motifs in their respective 5'-UTR without involvement of any viral protein. Further investigation pointed to a different role of TAR RNA, which was much inhibitory for HIV-2 translation. Altogether, these data highlight important structural and functional differences between these two human pathogens.
authors: INSERM U758, Lyon F-69364, France.
authors: Ricardo
authors: R
authors: Soto-Rifo
authors: Taran
authors: T
authors: Limousin
authors: Paulina S
authors: PS
authors: Rubilar
authors: Emiliano P
authors: EP
authors: Ricci
authors: Didier
authors: D
authors: Décimo
authors: Olivier
authors: O
authors: Moncorgé
authors: Mary-Anne
authors: MA
authors: Trabaud
authors: Patrice
authors: P
authors: André
authors: Andrea
authors: A
authors: Cimarelli
authors: Théophile
authors: T
authors: Ohlmann
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D002460:Cell Line; D016679:Genome, Viral; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D014176:Protein Biosynthesis; D011533:Proviruses / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry*; D012270:Ribosomes / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000096:biosynthesis / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus