title: Creation of a lipase highly selective for trans fatty acids by protein engineering.
abstract: Sorting out: Protein engineering of lipase CAL-A led to the discovery of mutants with excellent chemoselectivity for the removal of trans and saturated fatty acids from partially hydrogenated vegetable oil. These fatty acids, identified as a major risk factor for human health, can now be removed by enzyme catalysis.
authors: Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald, Germany.
authors: Henrike B
authors: HB
authors: Brundiek
authors: Andrew S
authors: AS
authors: Evitt
authors: Robert
authors: R
authors: Kourist
authors: Uwe T
authors: UT
authors: Bornscheuer
mesh_terms: D002175:Candida / Q000201:enzymology* / Q000235:genetics; D006865:Hydrogenation; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D015202:Protein Engineering; D044242:Trans Fatty Acids / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D044242:Trans Fatty Acids; D008049:Lipase