title: Balanced steady-state free precession fMRI with intravascular susceptibility contrast agent.
abstract: One major challenge in echo planar imaging-based functional MRI (fMRI) is the susceptibility-induced image distortion. In this study, a new cerebral blood volume-weighted fMRI technique using distortion-free balanced steady-state free precession (bSSFP) sequence was proposed and its feasibility was investigated in rat brain at 7 Tesla. After administration of intravascular susceptibility contrast agent (monocrystalline iron oxide nanoparticle [MION] at 15 mg/kg), unilateral visual stimulation was presented using a block-design paradigm. With repetition time/echo time = 3.8/1.9 ms and α = 18°, bSSFP fMRI was performed and compared with the conventional cerebral blood volume-weighted fMRI using post-MION gradient echo and spin echo echo planar imaging. The results showed that post-MION bSSFP fMRI provides comparable sensitivity but with no severe image distortion and signal dropout. Robust negative responses were observed during stimulation and activation patterns were in excellent agreement with known neuroanatomy. Furthermore, the post-MION bSSFP signal was observed to decrease significantly during hypercapnia challenge, indicating its sensitivity to cerebral blood volume changes. These findings demonstrated that post-MION bSSFP fMRI is a promising alternative to conventional cerebral blood volume-weighted fMRI. This technique is particularly suited for fMRI investigation of animal models at high field.
authors: Laboratory of Biomedical Imaging and Signal Processing, Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong SAR, China.
authors: Iris Y
authors: IY
authors: Zhou
authors: Matthew M
authors: MM
authors: Cheung
authors: Condon
authors: C
authors: Lau
authors: Kevin C
authors: KC
authors: Chan
authors: Ed X
authors: EX
authors: Wu
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology*; D003287:Contrast Media / Q000008:administration & dosage; D005071:Evoked Potentials / Q000502:physiology*; D005290:Ferric Compounds / Q000008:administration & dosage*; D007269:Injections, Intra-Arterial; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide