title: Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice.
abstract: Increasing evidence indicates that microRNAs (miRNAs) may be critical players in spermatogenesis. The miRNA expression profiles of THY1(+)-enriched undifferentiated spermatogonia were characterized, and members of Mir-17-92 (Mirc1) and its paralog Mir-106b-25 (Mirc3) clusters are significantly downregulated during retinoic acid-induced spermatogonial differentiation, both in vitro and in vivo. The repression of microRNA clusters Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3) by retinoic acid in turn potentially upregulates the expression of Bim, Kit, Socs3, and Stat3. The male germ cell-specific Mir-17-92 (Mirc1) knockout mice exhibit small testes, a lower number of epididymal sperm, and mild defect in spermatogenesis. Absence of Mir-17-92 (Mirc1) in male germ cells dramatically increases expression of Mir-106b-25 (Mirc3) cluster miRNAs in the germ cells. These results suggest that Mir-17-92 (Mirc1) cluster and Mir-106b-25 (Mirc3) cluster miRNAs possibly functionally cooperate in regulating spermatogonial development.
authors: School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA.
authors: Ming-Han
authors: MH
authors: Tong
authors: Debra Ann
authors: DA
authors: Mitchell
authors: Samantha Dawn
authors: SD
authors: McGowan
authors: Ryan
authors: R
authors: Evanoff
authors: Michael D
authors: MD
authors: Griswold
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D035683:MicroRNAs; C543896:Mirn106 microRNA, mouse; D014212:Tretinoin