title: The actin-specific reagent jasplakinolide induces apoptosis in primary rat parotid acinar cells.
abstract: Jasplakinolide is a reagent that stabilizes and polymerizes actin filaments and is a commonly used tool in cell biology. In primary rat parotid acinar cells, jasplakinolide partially inhibited the release of amylase induced by β-adrenergic receptor activation, as previously reported. However, in confocal microscopic observation with fluorescence conjugated anti-actin antibody, the jasplakinolide-treated cells not only showed decreased fluorescence intensity and aggregation of cortical F-actin but also revealed events characteristic of apoptosis such as cell shrinkage, membrane blebbing and apoptotic body formation. Such characteristic events of apoptosis were confirmed by transmission electron microscopy. The occurrence of apoptosis in jasplakinolide-treated cells was further confirmed by biochemical analysis: a DNA ladder was detected by electrophoresis, and DNA fragmentation was revealed using ELISA with an antibody to single-stranded DNA. Moreover, the degradation of fodrin was detected in jasplakinolide-treated cells by Western blotting, and the K(+) release induced by the fluid secretagogue carbachol was impaired. Taken together, these results demonstrate that jasplakinolide induces apoptosis and suppresses the secretory functions of rat parotid acinar cells.
authors: Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan. fukushima.miwako@nihon-u.ac.jp
authors: Miwako
authors: M
authors: Matsuki-Fukushima
authors: Sadamitsu
authors: S
authors: Hashimoto
authors: Masataka
authors: M
authors: Murakami
authors: Yorimasa
authors: Y
authors: Ogata
authors: Junko
authors: J
authors: Fujita-Yoshigaki
authors: Takanori
authors: T
authors: Narita
authors: Hiroshi
authors: H
authors: Sugiya
mesh_terms: D061354:Acinar Cells / Q000187:drug effects* / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000681:Amylases / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002217:Carbachol / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000378:metabolism; D047630:Depsipeptides / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010306:Parotid Gland / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013049:Spectrin
chemical_list: D000199:Actins; D000681:Amylases; D002217:Carbachol; D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D047630:Depsipeptides; D008840:Microfilament Proteins; D011943:Receptors, Adrenergic, beta; D013049:Spectrin; C057531:jasplakinolide