title: Erythrocyte oxidative damage in rat treated with CCl4: protective role of vanillin.
abstract: Owing to the presence of hemoglobin and polyunsaturated fatty acids, erythrocytes are a convenient model to understand membrane oxidative damage induced by various xenobiotic pro-oxidants. This study investigated the antioxidant activity of vanillin, a naturally occurring food-flavoring agent, against carbon tetrachloride (CCl(4))-induced erythrocyte damages in Wistar albino rats. A single injection of CCl(4) (1 ml/kg, intraperitoneally [i.p.]) caused a significant induction of oxidative damage as evidenced by increased thiobarbituric acid reactive substances, protein carbonyl levels and osmotic fragility accompanied with a significant decrease in Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities. Furthermore, catalase and superoxide dismutase activities were significantly elevated, while glutathione levels, glutathione-S-transferase and glutathione peroxidase activities were markedly reduced in the erythrocytes of CCl(4)-treated rats. Pretreatment of rats with vanillin (150 mg/kg/day, i.p.) for 3 consecutive days before CCl(4) injection protected erythrocytes against the increase of lipid peroxidation and degradation of membrane proteins compared to CCl(4)-treated rats and exhibited marked prevention against CCl(4)-induced oxidative stress, alterations of membrane-bound enzymes as well as erythrocyte osmotic fragility. Our results suggest that vanillin plays a protective and curative role against the harmful effects of CCl(4) on erythrocytes, thus ensuring membrane cell integrity.
authors: Animal Physiology Laboratory, University of Sfax, Sfax, Tunisia. mohamed.makni@gmail.com
authors: Mohamed
authors: M
authors: Makni
authors: Yassine
authors: Y
authors: Chtourou
authors: Hamadi
authors: H
authors: Fetoui
authors: El Mouldi
authors: el M
authors: Garoui
authors: Mohamed
authors: M
authors: Barkallah
authors: Chama
authors: C
authors: Marouani
authors: Choumous
authors: C
authors: Kallel
authors: Najiba
authors: N
authors: Zeghal
mesh_terms: D000251:Adenosine Triphosphatases / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001547:Benzaldehydes / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D006403:Hematologic Tests; D006461:Hemolysis / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009996:Osmotic Fragility / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001547:Benzaldehydes; D008315:Malondialdehyde; C100058:vanillin; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D000251:Adenosine Triphosphatases