title: HLA-class II and class I genotypes among Japanese children with Type 1A diabetes and their families.
abstract: OBJECTIVE
  To determine the HLA-DRB1, DQB1, DPB1, A, C, and B genotypes among Japanese children with autoimmune type 1 diabetes.
  
  
  METHODS
  Four hundred and thirty patients who were GADAb and/or IA-2Ab-positive (Type 1A) were recruited from 37 medical centers as part of a nationwide multicenter collaborative study. DNA samples from 83 siblings of the children with Type 1A diabetes and 149 parent-child trios were also analyzed. A case-control study and a transmission disequilibrium test (TDT) were then performed.
  
  
  RESULTS
  The susceptible and protective DRB1 and DQB1 alleles and haplotypes were confirmed. DPB1 alleles unique to the Japanese population and those common to multiple ethnic groups were also present. A linkage disequilibrium (LD) analysis showed both susceptible and protective haplotypes. The TDT did not reveal any alleles that were transmitted preferentially from the mother or father to children with Type 1A. Homozygosity for DRB1-09:01-DQB1-03:03 and heterozygosity for DRB1-04:05-DQB1-04:01 and DRB1-08:02-DQB1-03:02 were associated with an extremely high risk of Type 1A. A comparison of children with Type 1A and their parents and siblings suggested a dose effect of susceptible DRB1-DQB1 haplotypes and an effect of protective alleles on immunological pathogenesis. DRB1-09:01 appeared to be strongly associated with an early onset in preschool children with Type 1A diabetes.
  
  
  CONCLUSIONS
  This study demonstrated the characteristic association of HLA-class II and class I genes with Type 1A diabetes among Japanese children. A TDT did not reveal the genomic imprinting of HLA-class II and class I genes in Type 1A diabetes.
authors: Department of Pediatrics, Tokyo Women's Medical University Medical Center East, Tokyo, Japan. sghrsgpd@dnh.twmu.ac.jp
authors: Shigetaka
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authors: Sugihara
authors: Tsutomu
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authors: Ogata
authors: Tomoyuki
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authors: Kawamura
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authors: Urakami
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authors: Takemoto
authors: Nobuyuki
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authors: Kikuchi
authors: Noriyuki
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authors: Takubo
authors: Kohji
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authors: Tsubouchi
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authors: Horikawa
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authors: Kobayashi
authors: Yoshihito
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authors: Kasahara
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authors: Kikuchi
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authors: Koike
authors: Takahiro
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authors: Mochizuki
authors: Kanshi
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authors: Minamitani
authors: Ryuzo
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authors: Takaya
authors: Hiroshi
authors: H
authors: Mochizuki
authors: Aki
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authors: Nishii
authors: Ichiro
authors: I
authors: Yokota
authors: Zenro
authors: Z
authors: Kizaki
authors: Tetsuo
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authors: Mori
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authors: Shimura
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authors: Mukai
authors: Nobuo
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authors: Matsuura
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authors: Fujisawa
authors: Kenji
authors: K
authors: Ihara
authors: Kitaro
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authors: Kosaka
authors: Rika
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authors: Kizu
authors: Toshikazu
authors: T
authors: Takahashi
authors: Satoshi
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authors: Matsuo
authors: Keiichi
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authors: Hanaki
authors: Yutaka
authors: Y
authors: Igarashi
authors: Goro
authors: G
authors: Sasaki
authors: Shun
authors: S
authors: Soneda
authors: Shinichi
authors: S
authors: Teno
authors: Susumu
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authors: Kanzaki
authors: Hiroh
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authors: Saji
authors: Katsushi
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authors: Tokunaga
authors: Shin
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authors: Amemiya
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics* / Q000706:statistics & numerical data; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D003922:Diabetes Mellitus, Type 1 / Q000145:classification / Q000208:ethnology / Q000235:genetics*; D005190:Family; D005260:Female; D005805:Genes, MHC Class I / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male