title: Probing the interaction of human serum albumin with norfloxacin in the presence of high-frequency electromagnetic fields: fluorescence spectroscopy and circular dichroism investigations.
abstract: The present study describes an investigation by fluorescence quenching, circular dichroism and UV-visible spectroscopy of the interaction between norfloxacin (NRF) and human serum albumin (HSA) in the presence of electromagnetic fields (EMFs). The results obtained from this study indicated that NRF had a strong ability to quench HSA at λ(ex) = 280 nm. In addition, a slight blue shift occurred, which suggested that the microenvironment of the protein became more hydrophobic after addition of NRF. The interaction between the NRF and HSA, whether in the absence or presence of an EMF, was considered to be a static quenching mechanism. Moreover, synchronous fluorescence demonstrated that the microenvironment around Trp became modified. Data of HSA-NRF in the presence of EMFs between 1 Hz-1 MHz confirmed the results of quenching and blue shifts. Corresponding Stern-Volmer plots were also drawn and the resultant Ksv and kq values were compared. Moreover, the binding parameters, including the number of binding sites, the binding constant and the distance, r, between donor and acceptor, were calculated based on Förster's non-radiative energy transfer theory. According to far and near UV-CD, the formation of the complex caused changes of the secondary and tertiary structures of HSA. The obtained results are significant for patients who are subjected to high-frequency radiation as this was found to reduce the affinity of NRF to HSA.
authors: Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, 9175687119, Iran.
authors: Olga
authors: O
authors: Azimi
authors: Zahra
authors: Z
authors: Emami
authors: Hanieh
authors: H
authors: Salari
authors: Jamshidkhan
authors: J
authors: Chamani
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism / Q000379:methods*; D004574:Electromagnetic Fields; D004735:Energy Transfer; D006801:Humans; D007700:Kinetics; D008027:Light; D009643:Norfloxacin / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D012709:Serum Albumin; D009643:Norfloxacin