title: [Protective effects and mechanism of hyperin on CoCl2-induced PC12 cells].
abstract: OBJECTIVE
  To investigate the neuroprotective effects and mechanism of hyperin on CoCl2-induced hypoxic/ischemic PC12 cells.
  
  
  METHODS
  CoCl2 was used to treat rat pheochromocytoma cell line (PC12) to investigate the protective effects of different concentrations of hyperin.
  
  
  RESULTS
  Hyperin could significantly inhibit CoCl2-induced cytotoxicity and apoptosis on PC12 cells in a dose-dependent manner, by inhibiting reactive oxygen species (ROS) production, increasing mitochondrial membrane potential (MMP), and inactivating caspase-3 and poly ADP-ribose polymerase (PARP).
  
  
  CONCLUSIONS
  Hyperin could inhibit CoCl2-induced cytotoxicity and apoptosis on PC12 cells, show neuroprotective effects on hypoxic/ischemic neural injuries.
authors: Research Studio of Integration of Traditional and Western Medicine, First Hospital, Peking University, Beijing 100034, China.
authors: Kewu
authors: K
authors: Zeng
authors: Xuemei
authors: X
authors: Wang
authors: Hong
authors: H
authors: Fu
authors: Gengxin
authors: G
authors: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002477:Cells / Q000166:cytology* / Q000187:drug effects; D003035:Cobalt / Q000633:toxicity*; D020902:Hypericum / Q000737:chemistry*; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D003035:Cobalt; C021304:hyperoside; D011794:Quercetin; C018021:cobaltous chloride