title: 1,3-Propanediol production from glycerol by a newly isolated Trichococcus strain.
abstract: A coccal bacterium (strain ES5) was isolated from methanogenic bioreactor sludge with glycerol as the sole energy and carbon source. Strain ES5 fermented glycerol to 1,3-propanediol as main product, and lactate, acetate and formate as minor products. The strain was phylogenetically closely related to Trichococcus flocculiformis; the rRNA gene sequence similarity was 99%. However, strain ES5 does not show the typical growth in chains of T. flocculiformis. Moreover, T. flocculiformis does not ferment glycerol. Strain ES5 used a variety of sugars for growth. With these substrates, lactate, acetate and formate were the main products, while 1,3-propanediol was not formed. The optimum growth temperature of strain ES5 ranges from 30-37°C, but like several other Trichoccoccus strains, strain ES5 is able to grow at low temperature (< 10°C). Therefore, strain ES5 may be an appropriate catalyst for the biotechnological production of 1,3-propanediol from glycerol at low ambient temperature.
authors: Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
authors: Antonie H
authors: AH
authors: van Gelder
authors: Rozelin
authors: R
authors: Aydin
authors: M Madalena
authors: MM
authors: Alves
authors: Alfons J M
authors: AJ
authors: Stams
mesh_terms: D000085:Acetates / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002244:Carbon / Q000378:metabolism; D056568:Carnobacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D005561:Formates / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011409:Propylene Glycols / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D013696:Temperature
chemical_list: D000085:Acetates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005561:Formates; D011409:Propylene Glycols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C030544:formic acid; D019344:Lactic Acid; C041787:1,3-propanediol; D002244:Carbon; D005990:Glycerol