title: Masseter muscle fibre changes following reduction of masticatory function.
abstract: This study evaluated histological changes in masseter muscle fibres following reduced masticatory function by injection of botulinum toxin type A (BTX). Sixty 30-day-old Long-Evans male rats were randomly separated into four groups (15 per group): group I BTX masseter, 25U/ml (0.04ml each muscle) BTX was injected in bilateral masseter muscle whilst bilateral temporalis muscles received an equal amount of normal saline; group II BTX temporalis, 25U/ml (0.04ml each muscle) BTX was injected in bilateral temporalis muscle whilst bilateral masseter muscle received an equal amount of normal saline; group III BTX temporalis and masseter, bilateral temporalis and masseter were given 25U/ml (0.04ml each muscle) BTX; group IV normal saline (control), bilateral temporalis and masseter were given normal saline (0.04ml each muscle). After 45 days, the rats were killed, the muscles dissected and mean muscle mass recorded. The superficial masseter muscles were immunohistochemically analysed. Fibre sizes in group III were bigger than those in other groups. There was a small percentage of type IIa fibres in group III. Reduction in muscle fibre size and transition of muscle fibre subtypes from type IIa to IIx or IIb fibres may occur due to reduced masticatory function.
authors: Graduate Institute of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan. cytsai@tmu.edu.tw
authors: C Y
authors: CY
authors: Tsai
authors: Y C
authors: YC
authors: Lin
authors: B
authors: B
authors: Su
authors: L Y
authors: LY
authors: Yang
authors: W C
authors: WC
authors: Chiu
mesh_terms: D000818:Animals; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage / Q000494:pharmacology; D007273:Injections, Intramuscular; D008297:Male; D008406:Masseter Muscle / Q000187:drug effects / Q000473:pathology*; D008409:Mastication / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000473:pathology; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000473:pathology*; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects / Q000473:pathology; D018995:Myosin Heavy Chains / Q000032:analysis; D009465:Neuromuscular Agents / Q000008:administration & dosage / Q000494:pharmacology; D009929:Organ Size; D011897:Random Allocation; D051381:Rats; D013703:Temporal Muscle / Q000187:drug effects / Q000473:pathology
chemical_list: D009465:Neuromuscular Agents; D019274:Botulinum Toxins, Type A; D018995:Myosin Heavy Chains