title: Superoxide dismutase and catalase activities in rat hippocampus pretreated with garcinielliptone FC from Platonia insignis.
abstract: BACKGROUND
  Platonia insignis Mart. (Clusiaceae), commonly known as "bacuri," is a timber and fruit native species of the Brazilian Amazon. Some plants of the Clusiaceae family have their pharmacological properties associated with the presence of xanthone and polycyclic polyprenylated acylphloroglucinols derivatives, which have antioxidant and anticarcinogenic activities.
  
  
  OBJECTIVE
  The aim of this study was to assess the in vivo potential of extracts, fractions, and garcinielliptone FC isolated from of Platonia insignis seeds as a natural antioxidant.
  
  
  METHODS
  Male Wistar rats (250-280 g; 2 months old) were treated with Tween 80 0.05% dissolved in 0.9% saline (i.p, vehicle - control group), ethanol extract (EE), hexane extract (HE), dichloromethane fraction (DMF), ethyl acetate fraction (EAF), and garcinielliptone FC (GFC) isolated from P. insignis at doses 2 mg/kg (i.p.). All groups were observed for 24 h after the treatment. The antioxidant enzymatic activities [superoxide dismutase (SOD) and catalase (CAT)] were measured using spectrophotometric methods.
  
  
  RESULTS
  There were no marked alterations in SOD and CAT activities in rat hippocampus after pretreatment with EE, HE, DMF, EAF, and GFC. However, the pretreatment with GFC induced a significantly increase of 13, 17, 19, and 13% in SOD activities when compared to EE, HE, DMF, or EAF groups, respectively.
  
  
  CONCLUSIONS
  Our findings strongly support the hypothesis that GFC isolated from P. insignis has a significant potential to be used as a natural antioxidant agent probably due to the modulation of enzymatic activity of hippocampal SOD.
authors: Laboratory of Genetic Toxicology, Post-Graduation Program in Genetic and Applied Toxicology, Lutheran University of Brazil, Canoas, RS, Brazil.
authors: Joaquim S
authors: JS
authors: da Costa Júnior
authors: Antonia Amanda C
authors: AA
authors: de Almeida
authors: Jéssica Pereira
authors: JP
authors: Costa
authors: Antonia Maria
authors: AM
authors: das Graças Lopes Citó
authors: Jenifer
authors: J
authors: Saffi
authors: Rivelilson Mendes
authors: RM
authors: de Freitas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism*; D028084:Clusiaceae / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; C573355:garcinielliptone FC; D002374:Catalase; D013482:Superoxide Dismutase