title: Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes.
abstract: The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient sensing and immune function. In CD4+ T cells, mTOR has been shown to play a critical role in regulating effector and regulatory T cell differentiation as well as the decision between full activation versus the induction of anergy. In this chapter, we describe how our group has employed the Cre-lox technology to genetically delete components of the mTOR signaling complex in T cells. This has enabled us to specifically interrogate mTOR function in T cells both in vitro and in vivo. We also describe techniques used to assay immune function and signaling in mTOR-deficient T cells at the single-cell level.
authors: Sidney-Kimmel Comprehensive Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors: Greg M
authors: GM
authors: Delgoffe
authors: Jonathan D
authors: JD
authors: Powell
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016207:Cytokines / Q000032:analysis; D017353:Gene Deletion; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000379:methods*; D020123:Sirolimus / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D058570:TOR Serine-Threonine Kinases / Q000235:genetics*
chemical_list: D016207:Cytokines; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus