title: Echinicola shivajiensis sp. nov., a novel bacterium of the family "Cyclobacteriaceae" isolated from brackish water pond.
abstract: Strain AK12(T), an orange pigmented Gram-negative, rod shaped, non-motile bacterium, was isolated from a mud sample collected from a brackish water pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant fatty acids were iso-C(15:0) (42.7%), iso-C(17:0) 3OH (13.2%), C(16:1ω7c)/C(16:1ω6c) (summed feature 3) (8.0%), iso-C(17:1) I/anteiso-C(17:1) B (summed feature 4) (6.1%) and iso-C(17:1ω9c)/C(16:0) 10-methyl (summed feature 9) (9.4%). Strain AK12(T) contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids as the polar lipids. The G + C content of DNA of the strain AK12(T) was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12(T) was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain AK12(T) clustered with E. vietnamensis and together with E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the current polyphasic study, strain AK12(T) is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12(T) (= MTCC 11083(T) = JCM 17847(T)).
authors: National Institute of Oceanography (CSIR), Regional Centre, P B No. 1913, Dr. Salim Ali Road, Kochi, Kerala, India. tanuku@nio.org
authors: T N R
authors: TN
authors: Srinivas
authors: B
authors: B
authors: Kailash Tryambak
authors: P
authors: P
authors: Anil Kumar
mesh_terms: D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010641:Phenotype; D061005:Ponds / Q000382:microbiology*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D009584:Nitrogen