title: Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5.
abstract: We describe the culture and use of MLO-Y4 cells in studies of gene expression, response to fluid flow, and dendrite growth. We also describe how to use the MLO-A5 cells as a model of osteoblast to osteocyte -differentiation and how to study their mineralization. These studies serve as a beginning point to study osteocyte functions and molecular mechanisms responsible for these functions.
authors: Department of Oral Biology, University of Missouri at Kansas City, Kansas City, USA.
authors: Jennifer
authors: J
authors: Rosser
authors: Lynda F
authors: LF
authors: Bonewald
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression; D057446:Hydrodynamics; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000008:administration & dosage; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering