title: A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology.
abstract: BACKGROUND
  Neonatal alloimmune thrombocytopenia is mostly due to the presence of maternal antibodies against the fetal platelet antigen HPA-1a on the platelet integrin GPIIb-IIIa. Accurate detection of anti-HPA-1a antibodies in the mother is, therefore, critical. Current diagnostic assays rely on the availability of pools of human platelets that vary according to donors and blood centers. There is still no satisfactory standardization of these assays.
  
  
  METHODS
  Peptide aptamer was used to detect and identify HPA-1a-specific antibodies in human serum that do not require human platelets. A peptide aptamer library was screened using an anti-HPA-1a human monoclonal antibody as a bait to isolate an aptamer that mimics the human platelet antigen HPA-1a.
  
  
  RESULTS
  This is the first report in platelet immunology of the use of a peptide aptamer for diagnostic purposes. This assay gives better results than the MAIPA currently in use, detecting around 90% of the expected alloantibodies.
  
  
  CONCLUSIONS
  This assay could help define a standard for the quantitation of anti-HPA antibodies. This report also demonstrates that peptide aptamers can potentially detect a variety of biomarkers in body fluids; this is of particular interest for diagnostic purposes.
authors: Université de Lyon, IBCP, UMR 5086 CNRS-Université Lyon, Lyon, France.
authors: Julien
authors: J
authors: Thibaut
authors: Yves
authors: Y
authors: Mérieux
authors: Dominique
authors: D
authors: Rigal
authors: Germain
authors: G
authors: Gillet
mesh_terms: D000595:Amino Acid Sequence; D000888:Antibodies, Anti-Idiotypic / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000936:Antigen-Antibody Complex; D016824:Antigens, Human Platelet / Q000276:immunology*; D052157:Aptamers, Nucleotide; D001792:Blood Platelets / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay; D047468:Immunoprecipitation; D007231:Infant, Newborn; D039661:Integrin beta3; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology*; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D016824:Antigens, Human Platelet; D052157:Aptamers, Nucleotide; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; D010446:Peptide Fragments; D019039:Platelet Glycoprotein GPIIb-IIIa Complex