title: Effects of electroacupuncture pretreatment on inflammatory response and acute kidney injury in endotoxaemic rats.
abstract: This study investigated the effect of electroacupuncture pretreatment on the lipopolysaccharide (LPS)-induced inflammatory response and on acute kidney injury in adult male pathogen-free Wistar rats. Rats received electroacupuncture at the Zusanli (ST36) and Neiguan (PC6) acupoints,  or electrical stimulation at sham points, for 30 min before stimulation with either 5 mg/kg LPS intravenously or normal saline. Plasma cytokines, plasma nitrite, renal inducible nitric oxide synthase (iNOS) and nuclear factor κB (NF-κB) activity were assessed 240 min after LPS  or normal saline injection. Blood urea nitrogen (BUN), creatinine (Cr) and histopathological score for renal tubular damage were also measured. Electroacupuncture pretreatment significantly decreased LPS-induced plasma tumour necrosis factor-α and interleukin (IL)-1β, increased  plasma IL-10, and decreased plasma nitrite, renal iNOS and NF-κB activity. It also significantly decreased LPS-induced BUN, Cr and the renal histopathological score. These findings suggest that electroacupuncture pretreatment at the ST36 and PC6 acupoints attenuated the LPS-induced inflammatory  response and mitigated acute kidney injury.
authors: Department of Anaesthesiology, The Third Affiliated Hospital, Harbin Medical University, Harbin, China.
authors: G
authors: G
authors: Gu
authors: Z
authors: Z
authors: Zhang
authors: G
authors: G
authors: Wang
authors: F
authors: F
authors: Han
authors: L
authors: L
authors: Han
authors: K
authors: K
authors: Wang
authors: J
authors: J
authors: Liu
authors: W
authors: W
authors: Li
mesh_terms: D015669:Acupuncture Points; D058186:Acute Kidney Injury / Q000097:blood / Q000139:chemically induced* / Q000517:prevention & control; D000818:Animals; D001794:Blood Pressure; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D016207:Cytokines / Q000097:blood; D015671:Electroacupuncture; D019446:Endotoxemia / Q000097:blood / Q000139:chemically induced* / Q000517:prevention & control; D007249:Inflammation / Q000097:blood / Q000139:chemically induced* / Q000517:prevention & control; D007668:Kidney / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009573:Nitrites; D003404:Creatinine; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat