title: Diagnostic use of Burkholderia pseudomallei selective media in a low prevalence setting.
abstract: Routine use of selective media improves diagnosis of Burkholderia pseudomallei, but resources may be limited in endemic developing countries. To maximise yield in the relatively low-prevalence setting of Kuala Lumpur, Malaysia, B. pseudomallei selective agar and broth were compared with routine media for 154 respiratory specimens from patients with community-acquired disease. Selective media detected three additional culture-positive specimens and one additional melioidosis patient, at a consumables cost of US$75. Burkholderia pseudomallei was not isolated from 74 diabetic foot ulcer samples. Following careful local evaluation, focused use of selective media may be cost-effective.
authors: Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors: Baharudin
authors: B
authors: Roesnita
authors: Sun Tee
authors: ST
authors: Tay
authors: Savithri D
authors: SD
authors: Puthucheary
authors: I-Ching
authors: IC
authors: Sam
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods*; D016957:Burkholderia pseudomallei / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D017719:Diabetic Foot / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D008554:Melioidosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D003470:Culture Media; D000362:Agar