title: Intraspinal injection of human umbilical cord blood-derived cells is neuroprotective in a transgenic mouse model of amyotrophic lateral sclerosis.
abstract: BACKGROUND
  Amyotrophic lateral sclerosis (ALS) is characterized by progressive degeneration of motor neurons in the spinal cord, brain stem and motor cortex and has only marginal therapeutic options. Adult stem cells have recently come into the focus of neurological research. While replacement of motor neurons by stem cells currently appears not feasible, there is evidence that non-neuronal cells can be neuroprotective.
  
  
  OBJECTIVE
  Therefore, we evaluated the effects of direct intraspinal administration of human umbilical cord blood cells in a G93A transgenic mouse model of ALS before (day 40) and after symptom onset (day 90).
  
  
  METHODS
  Treatment effects were assessed by survival analysis, behavioral tests, histological and biochemical analyses.
  
  
  RESULTS
  Treatment at early stages increased survival, led to significant improvements in motor performance and significantly reduced motor neuron loss and astrogliosis in the spinal cord. Interestingly females tended to respond better to treatment than males.
  
  
  CONCLUSIONS
  This study confirms the neuroprotective potential of human umbilical cord blood cells and encourages further investigations.
authors: Department of Neurology, Hannover Medical School, Hannover, Germany. Knippenberg.Sarah@mh-hannover.de
authors: Sarah
authors: S
authors: Knippenberg
authors: Nadine
authors: N
authors: Thau
authors: Kerstin
authors: K
authors: Schwabe
authors: Reinhard
authors: R
authors: Dengler
authors: Axel
authors: A
authors: Schambach
authors: Ralf
authors: R
authors: Hass
authors: Susanne
authors: S
authors: Petri
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D000818:Animals; D002470:Cell Survival; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005911:Gliosis / Q000473:pathology / Q000517:prevention & control; D006801:Humans; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009043:Motor Activity; D009046:Motor Neurons / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology*; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D016896:Treatment Outcome
chemical_list: C000606290:SOD1 protein, human; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1