title: Qualitative difference between "bulb" membranes and other vacuolar membranes.
abstract: "Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75.
authors: Molecular Membrane Biology Laboratory, RIKEN Advanced Science Institute, Wako, Japan. chiezo@riken.jp
authors: Chieko
authors: C
authors: Saito
authors: Tomohiro
authors: T
authors: Uemura
authors: Chie
authors: C
authors: Awai
authors: Takashi
authors: T
authors: Ueda
authors: Hiroshi
authors: H
authors: Abe
authors: Akihiko
authors: A
authors: Nakano
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000648:ultrastructure*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000648:ultrastructure*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050765:Qa-SNARE Proteins; C108430:Vam3 protein, Arabidopsis; D049452:Green Fluorescent Proteins; D000217:Acyltransferases; C506684:TIP1 protein, Arabidopsis; C556156:RabG3b protein, Arabidopsis; D020691:rab GTP-Binding Proteins