title: Photoactivated cationic alkyl-substituted porphyrin binding to g4-RNA in the 5'-UTR of KRAS oncogene represses translation.
abstract: The KRAS transcript is characterized by a 192-nt 5'-UTR containing repetitive runs of two-guanines which can fold in several G-quadruplexes. These folded structures have a high affinity for the cationic porphyrin tri-meso(N-methyl-4-pyridyl), meso(N-tetradecyl-4-pyridyl) porphine (TMPyP4-C14), which efficiently penetrates cell membranes. Upon photoactivation TMPyP4-C14 induces a dramatic down-regulation of oncogenic KRAS and cell growth arrest in pancreatic cancer cells.
authors: Department of Medical and Biological Science, University of Udine, 33100 Udine, Italy.
authors: Mariangela
authors: M
authors: Faudale
authors: Susanna
authors: S
authors: Cogoi
authors: Luigi E
authors: LE
authors: Xodo
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D015536:Down-Regulation; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D011905:Genes, ras / Q000235:genetics*; D006147:Guanine / Q000737:chemistry; D006801:Humans; D010777:Photochemistry; D011166:Porphyrins / Q000737:chemistry* / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D012313:RNA / Q000737:chemistry*
chemical_list: D020121:5' Untranslated Regions; D011166:Porphyrins; D006147:Guanine; D012313:RNA; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)