title: Effect of maternal micronutrients (folic acid, vitamin B12) and omega 3 fatty acids on liver fatty acid desaturases and transport proteins in Wistar rats.
abstract: A disturbed fatty acid metabolism increases the risk of adult non-communicable diseases. This study examines the effect of maternal micronutrients on the fatty acid composition, desaturase activity, mRNA levels of fatty acid desaturases and transport proteins in the liver. Pregnant female rats were divided into 6 groups at 2 levels of folic acid both in the presence and absence of vitamin B(12). The vitamin B(12) deficient groups were supplemented with omega 3 fatty acid. An imbalance of maternal micronutrients reduces liver docosahexaenoic acid, increases Δ5 desaturase activity but decreases mRNA levels, decreases Δ6 desaturase activity but not mRNA levels as compared to control. mRNA level of Δ5 desaturase reverts back to the levels of the control group as a result of omega 3 fatty acid supplementation. Our data for the first time indicates that maternal micronutrients differentially alter the activity and expression of fatty acid desaturases in the liver.
authors: Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, India.
authors: Nisha S
authors: NS
authors: Wadhwani
authors: Rupali R
authors: RR
authors: Manglekar
authors: Kamini D
authors: KD
authors: Dangat
authors: Asmita V
authors: AV
authors: Kulkarni
authors: Sadhana R
authors: SR
authors: Joshi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D000090943:Delta-5 Fatty Acid Desaturase; D019587:Dietary Supplements; D000074026:Fatty Acid Binding Protein 3; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D018977:Micronutrients / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014805:Vitamin B 12 / Q000008:administration & dosage / Q000494:pharmacology; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D000090943:Delta-5 Fatty Acid Desaturase; C509948:FABP3 protein, rat; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D018977:Micronutrients; C493570:Slc27a1 protein, rat; D014815:Vitamins; D012313:RNA; D005492:Folic Acid; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase; D014805:Vitamin B 12