title: Molecular characterisation of isoniazid- and rifampicin-resistant  Mycobacterium tuberculosis in Central Tunisia.
abstract: The aim of our study was to genotypically characterise isoniazid (INH) and rifampicin (RMP) resistant Mycobacterium tuberculosis isolates in Sousse, Central Tunisia, using DNA sequencing and multispacer sequence typing (MST). The results show that 27/28 (96.4%) and 1/28 (3.6%)  INH-resistant isolates yielded respectively the kat G S315T and the inh A - 15C → T mutations. Two-thirds of RMP-resistant isolates yielded the rpo B D516V mutation and one sixth yielded either H526D or S531L mutations. Genotyping analysis revealed the multiclonal  spread of drug-resistant isolates in Central Tunisia. Data presented here complete the previously published map of resistant M. tuberculosis isolates and highlight their regional disparity in Tunisia.
authors: Laboratoire de Microbiologie et d'Immunologie, UR02/SP13, CHU Farhat Hached Sousse, Tunisie.
authors: I
authors: I
authors: Ben Kahla
authors: M
authors: M
authors: Marzouk
authors: M
authors: M
authors: Henry
authors: M
authors: M
authors: Bedotto
authors: S
authors: S
authors: Cohen-Bacrie
authors: W
authors: W
authors: Ben Selma
authors: J
authors: J
authors: Boukadida
authors: M
authors: M
authors: Drancourt
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods*; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology; D017422:Sequence Analysis, DNA / Q000379:methods; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology*; D014416:Tunisia / Q000453:epidemiology
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin