title: Transfer of foodborne pathogenic bacteria to non-inoculated beef fillets through meat mincing machine.
abstract: The aim of this study was to evaluate the transfer of pathogens population to non-inoculated beef fillets through meat mincing machine. In this regard, cocktails of mixed strain cultures of each Listeria monocytogenes, Salmonella enterica ser. Typhimurium and Escherichia coli O157:H7 were used for the inoculation of beef fillets. Three different initial inoculum sizes (3, 5, or 7 log CFU/g) were tested. The inoculated beef fillets passed through meat mincing machine and then, six non-inoculated beef fillets passed in sequence through the same mincing machine without sanitation. The population of each pathogen was measured. It was evident that, all non-inoculated beef fillets were contaminated through mincing with all pathogens, regardless the inoculum levels used. This observation can be used to cover knowledge gaps in risk assessments since indicates the potential of pathogen contamination and provides significant insights for the risk estimation related to cross-contamination, aiming thus to food safety enhancement.
authors: Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
authors: O S
authors: OS
authors: Papadopoulou
authors: N G
authors: NG
authors: Chorianopoulos
authors: E N
authors: EN
authors: Gkana
authors: A V
authors: AV
authors: Grounta
authors: K P
authors: KP
authors: Koutsoumanis
authors: G-J E
authors: GJ
authors: Nychas
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D019453:Escherichia coli O157 / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology / Q000379:methods*; D008089:Listeria monocytogenes / Q000302:isolation & purification; D008460:Meat / Q000032:analysis / Q000382:microbiology*; D018570:Risk Assessment; D019779:Salmonella enterica / Q000302:isolation & purification