title: Protein and antigen diversity in the vesicular fluid of Taenia solium cysticerci dissected from naturally infected pigs.
abstract: Cysticercosis caused by Taenia solium is a health threat for humans and pigs living in developing countries, for which there is neither a flawless immunodiagnostic test nor a totally effective vaccine. Suspecting of individual diversity of hosts and parasites as possible sources of the variations of the parasite loads among cysticercotic animals and of the limited success of such immunological applications as well as, we explored and measured both in nine cases of naturally acquired porcine cysticercosis. For this purpose, 2-Dimensional IgG immunoblots were performed by reacting the sera of each cysticercotic pig with the antigens contained in the vesicular fluid (VF) of their own cysticerci. We found an unexpectedly large diversity among the proteins and antigens contained in each of the nine VFs. Also diverse were the serum IgG antibody responses of the nine pigs, as none of their 2D- immunoblot images exhibited the same number of spots and resembled each other in only 6.3% to 65.3% of their features. So large an individual immunological diversity of the cysticercal antigens and of the infected pigs´ IgG antibody response should be taken into account in the design of immunological tools for diagnosis and prevention of cysticercosis and should also be considered as a possibly significant source of diversity in Taenia solium´s infectiveness and pathogenicity.
authors: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, A.P. 70228, México D.F 04510, México.
authors: Marcela
authors: M
authors: Esquivel-Velázquez
authors: Carlos
authors: C
authors: Larralde
authors: Julio
authors: J
authors: Morales
authors: Pedro
authors: P
authors: Ostoa-Saloma
mesh_terms: D000818:Animals; D003551:Cysticercosis / Q000469:parasitology*; D015180:Electrophoresis, Gel, Two-Dimensional; D015801:Helminth Proteins / Q000378:metabolism*; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D013552:Swine; D041201:Taenia solium / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D015801:Helminth Proteins; D020543:Proteome