title: The role of leptin receptor gene polymorphisms in determining the susceptibility and prognosis of NSCLC in Chinese patients.
abstract: OBJECTIVE
  Although the role of genetic polymorphisms of leptin receptor (LEPR) gene in several cancers has been documented, the association between polymorphisms of LEPR gene and lung cancer remains unknown.
  
  
  METHODS
  We recruited 744 patients histologically diagnosed as non-small cell lung cancer (NSCLC) and 832 controls in this study. Polymorphism analysis of LEPR gene was performed by PCR-restriction fragment length polymorphisms.
  
  
  RESULTS
  The Arg/Arg genotype and Arg allele frequency of the Gln223Arg in LEPR gene were significantly prevalent in NSCLC subjects than in controls (P < 0.05). The odd ratio (OR) for NSCLC in Arg/Arg genotype carriers was 3.12 (95% CI: 2.25-4.56, P = 0.0023, with Gln/Gln as reference). There were no significant differences in the genotype distributions and allele frequencies of Lys109Arg and Lys656Asn in LEPR gene between NSCLC cases and controls (All P > 0.05). The Arg/Arg carriers had higher cancer grade and higher TNM stage. Kaplan-Mier curve showed the Arg/Arg carriers had a poor prognosis than those with Gln/Arg and Gln/Gln genotype carriers. Cox proportional hazards regression models showed the hazard ratio (HR) for death associated with Arg/Arg genotype was 3.43 (95% CI: 2.45-5.92, compared with Gln/Gln carriers, P = 0.002). The other two SNPs of LEPR gene did not show this trend in the evaluation of their role in determining the prognosis of NSCLC subjects.
  
  
  CONCLUSIONS
  The results suggest the polymorphisms of Gln223Arg, rather than Lys109Arg and Lys656Asn, may be used as a molecular marker for progression and prognosis of NSCLC.
authors: Second Affiliated Hospital of Shandong University, BeiYuan Road 247, Jinan city, 250033, Shandong province, People's Republic of China. drliyuliang@yahoo.cn
authors: Yuliang
authors: Y
authors: Li
authors: Jianli
authors: J
authors: Geng
authors: Yongzheng
authors: Y
authors: Wang
authors: Qinghua
authors: Q
authors: Lu
authors: Yimeng
authors: Y
authors: Du
authors: Wujie
authors: W
authors: Wang
authors: Zheng
authors: Z
authors: Li
mesh_terms: D044466:Asian People; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D016016:Proportional Hazards Models; D054411:Receptors, Leptin / Q000235:genetics*
chemical_list: D054411:Receptors, Leptin