title: Erythrocyte remodeling in Plasmodium berghei infection: the contribution of SEP family members.
abstract: The malaria parasite Plasmodium largely modifies the infected erythrocyte through the export of proteins to multiple sites within the host cell. This remodeling is crucial for pathology and translocation of virulence factors to the erythrocyte surface. In this study, we investigated localization and export of small exported proteins/early transcribed membrane proteins (SEP/ETRAMPs), conserved within Plasmodium genus. This protein family is characterized by a predicted signal peptide, a short lysine-rich stretch, an internal transmembrane domain and a highly charged C-terminal region of variable length. We show here that members of the rodent Plasmodium berghei family are components of the parasitophorous vacuole membrane (PVM), which surrounds the parasite throughout the erythrocytic cycle. During P. berghei development, vesicle-like structures containing these proteins detach from the PVM en route to the host cytosol. These SEP-containing vesicles remain associated with the infected erythrocyte ghosts most probably anchored to the membrane skeleton. Transgenic lines expressing the green fluorescent protein appended to different portions of sep-coding region allowed us to define motifs required for protein export. The highly charged terminal region appears to be involved in protein-protein interactions.
authors: Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Rome, Italy.
authors: Chiara
authors: C
authors: Currà
authors: Tomasino
authors: T
authors: Pace
authors: Blandine M D
authors: BM
authors: Franke-Fayard
authors: Leonardo
authors: L
authors: Picci
authors: Lucia
authors: L
authors: Bertuccini
authors: Marta
authors: M
authors: Ponzi
mesh_terms: D000818:Animals; D004907:Erythrocyte Deformability / Q000235:genetics; D004910:Erythrocyte Membrane / Q000235:genetics / Q000378:metabolism; D004912:Erythrocytes / Q000502:physiology*; D015151:Immunoblotting; D008288:Malaria / Q000473:pathology*; D051379:Mice; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D010962:Plasmodium berghei; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D046912:Multiprotein Complexes; D015800:Protozoan Proteins