title: Voltage-dependent inactivation of the plasmodial surface anion channel via a cleavable cytoplasmic component.
abstract: Erythrocytes infected with malaria parasites have increased permeability to ions and various nutrient solutes, mediated by a parasite ion channel known as the plasmodial surface anion channel (PSAC). The parasite clag3 gene family encodes PSAC activity, but there may also be additional unidentified components of this channel. Consistent with a lack of clag3 homology to genes of other ion channels, PSAC has a number of unusual functional properties. Here, we report that PSAC exhibits an unusual form of voltage-dependent inactivation. Inactivation was readily detected in the whole-cell patch-clamp configuration after steps to negative membrane potentials. The fraction of current that inactivates, its kinetics, and the rate of recovery were all voltage-dependent, though with a modest effective valence (0.7±0.1 elementary charges). These properties were not affected by solution composition or charge carrier, suggesting inactivation intrinsic to the channel protein. Intriguingly, inactivation was absent in cell-attached recordings and took several minutes to appear after obtaining the whole-cell configuration, suggesting interactions with soluble cytosolic components. Inactivation could also be largely abolished by application of intracellular, but not extracellular protease. The findings implicate inactivation via a charged cytoplasmic channel domain. This domain may be tethered to one or more soluble intracellular components under physiological conditions.
authors: National Institute of Health, Rockville, MD, USA.
authors: Abdulnaser
authors: A
authors: Alkhalil
authors: Liang
authors: L
authors: Hong
authors: Wang
authors: W
authors: Nguitragool
authors: Sanjay A
authors: SA
authors: Desai
mesh_terms: D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials / Q000502:physiology*; D010963:Plasmodium falciparum / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D007473:Ion Channels; C505335:surface anion channel protein, Plasmodium falciparum