title: Osteoblast isolation from murine calvaria and long bones.
abstract: This chapter describes the isolation of primary mouse osteoblasts from adult mouse calvaria and long bones, as well as the process of isolation of bone cells from neonatal mouse calvaria. Osteoblasts from adult mouse bone are obtained as outgrowth from collagenase-treated bone pieces. Isolation of osteoblasts from neonatal calvaria is achieved by sequential enzymatic digestion of the bone matrix. Because of differences in origin and isolation method, each of the primary bone cell cultures described will have their own characteristics.
authors: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands.
authors: Astrid D
authors: AD
authors: Bakker
authors: Jenneke
authors: J
authors: Klein-Nulend
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D012886:Skull / Q000166:cytology* / Q000378:metabolism
chemical_list: D017364:Collagenases