title: Amino acid analysis via LC-MS method after derivatization with quaternary phosphonium.
abstract: (5-N-Succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP) is a highly sensitive and positively charged precolumn derivatization reagent for the analysis of amino acids in liquid chromatography-electrospray ionization-tandem mass spectrometry. The synthesis of this reagent and handling of the derivatization reaction are quite simple. It reacts with amino acids rapidly and with high efficiency. MS/MS analysis revealed that the SPTPP-derivatized amino acids formed strong product ions; thus, highly sensitive and selective detection is possible in the selected reaction monitoring mode. The limits of detection for the SPTPP-derivatized amino acids are in the sub-fmol range. The sensitivities of the derivatized amino acids increased about 500-fold, as compared to those of underivatized amino acids.
authors: Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, and Global COE Program, University of Shizuoka, Shizuoka, Japan.
authors: Shinsuke
authors: S
authors: Inagaki
authors: Toshimasa
authors: T
authors: Toyo'oka
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013388:Succinimides / Q000378:metabolism*; D014443:Tyrosine / Q000032:analysis / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000737:chemistry / Q000378:metabolism
chemical_list: C550832:(5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium; D000596:Amino Acids; D009943:Organophosphorus Compounds; D013388:Succinimides; D014443:Tyrosine; D005680:gamma-Aminobutyric Acid