title: Purification and characterization of a heteromultimeric glycoprotein from Artocarpus heterophyllus latex with an inhibitory effect on human blood coagulation.
abstract: Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XI(a) and α-XII(a).
authors: Department of Pathology, Institute of Medicine and School of Biochemistry, Institute of Science, Suranaree University of Technology, Thailand. jaruwan_siritape@yahoo.com
authors: Jaruwan
authors: J
authors: Siritapetawee
authors: Sompong
authors: S
authors: Thammasirirak
mesh_terms: D000925:Anticoagulants / Q000302:isolation & purification* / Q000494:pharmacology; D031622:Artocarpus / Q000737:chemistry*; D001777:Blood Coagulation; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D015956:Factor XIIa / Q000737:chemistry; D015945:Factor XIa / Q000737:chemistry; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology*; D006031:Glycosylation; D006801:Humans; D007526:Isoelectric Point; D007840:Latex / Q000737:chemistry*; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D055550:Protein Stability; D011517:Prothrombin Time; D015842:Serine Proteinase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013696:Temperature; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D006023:Glycoproteins; D007840:Latex; D015842:Serine Proteinase Inhibitors; D015945:Factor XIa; D015956:Factor XIIa