title: Development of 29 microsatellite markers for Osmanthus fragrans (Oleaceae), a traditional fragrant flowering tree of China.
abstract: OBJECTIVE
  Microsatellite markers were developed for a traditional fragrant flowering tree of China, Osmanthus fragrans, to investigate the genetic diversity of its wild populations and to facilitate the classification and identification of O. fragrans cultivars.
  
  
  RESULTS
  Using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol, 29 primer sets were identified in two wild populations. All primer pairs displayed polymorphism. The number of alleles per locus ranged from two to eight, with a mean of 3.9. The expected and observed heterozygosities ranged from 0.125 to 0.932 and from 0.083 to 0.917, respectively. The transferability of the 29 primer pairs was tested on O. serrulatus, O. delavayi, and O. yunnanensis (three individuals for each species). Eighteen (62.1%), 16 (55.2%), and 21 (72.4%) of them were successfully amplified in O. serrulatus, O. delavayi, and O. yunnanensis, respectively.
  
  
  CONCLUSIONS
  These markers will facilitate further studies on the population genetics of O. fragrans and the classification and identification of O. fragrans cultivars.
authors: Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang, China.
authors: Zhi-Rong
authors: ZR
authors: Zhang
authors: Deng-Mei
authors: DM
authors: Fan
authors: Shi-Quan
authors: SQ
authors: Guo
authors: De-Zhu
authors: DZ
authors: Li
authors: Zhi-Yong
authors: ZY
authors: Zhang
mesh_terms: D002681:China; D035264:Flowers / Q000502:physiology*; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D005821:Genetic Techniques; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D009812:Odorants; D029592:Oleaceae / Q000235:genetics*; D014197:Trees / Q000235:genetics*
chemical_list: D005819:Genetic Markers