title: Isolation, identification and characterization of a novel triazophos-degrading Bacillus sp. (TAP-1).
abstract: A novel triazophos-degrading Bacillus sp., TAP-1, was isolated from sewage sludge in a wastewater treating system of organophosphorus pesticide produced by Funong Group Co. in Jianou, Fujian, southeastern China. The isolate is a gram-positive and rod-shaped bacterium capable of hydrolyzing insecticide triazophos and was identified as a strain of Bacillus using polyphasic taxonomy combined with analysis of the morphological and physio-biochemical properties. TAP-1 could degrade triazophos through co-metabolism. When fed with nutrients such as yeast extract, peptone and glucose, TAP-1 could degrade 98.5% of TAP in the medium (100 mg/l) within 5 days. The optimal pH and temperature for the degradation were 6.5-8 and 32°C, respectively. An enzyme distribution experiment showed that the enzyme responsible for TAP degradation appeared to be intracellular.
authors: Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Mingqiang
authors: M
authors: Tang
authors: Minsheng
authors: M
authors: You
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D008969:Molecular Sequence Data; D063086:Organothiophosphates / Q000378:metabolism*; D010575:Pesticides / Q000378:metabolism*; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D014230:Triazoles / Q000378:metabolism*
chemical_list: D063086:Organothiophosphates; D010575:Pesticides; D012722:Sewage; D014230:Triazoles; C012584:triazophos