title: Dynamics and mechanisms of coupled protein folding and binding reactions.
abstract: Protein folding coupled to binding of a specific ligand is frequently observed in biological processes. In recent years numerous studies have addressed the structural properties of the unfolded proteins in the absence of their ligands. Surprisingly few time-resolved investigations on coupled folding and binding reactions have been published up to date and the dynamics and kinetic mechanisms of these processes are still only poorly understood. Especially, it is still unsolved for most systems which conformation of the protein is recognized by the ligand (conformational selection vs. folding-after-binding) and whether the ligand influences the folding kinetics. Here we review experimental methods, kinetic models and time-resolved experimental studies of coupled folding and binding reactions.
authors: Munich Center for Integrated Protein Science at the Chemistry Department, TU München, Lichtenbergstrasse 4, D-85747 Garching, Germany. t.kiefhaber@tum.de
authors: Thomas
authors: T
authors: Kiefhaber
authors: Annett
authors: A
authors: Bachmann
authors: Kristine Steen
authors: KS
authors: Jensen
mesh_terms: D006801:Humans; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008024:Ligands; D011506:Proteins