title: [Simultaneous determination of 17 preservatives and antioxidants in condiments using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry].
abstract: An ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous determination of 11 preservatives and 6 antioxidants in condiments. The condiment sample with low fat or middle fat was diluted with saturated NaCl solution (adjusting pH to 2 - 3 with phosphoric acid), then extracted with acetonitrile. The extract was purified using liquid-liquid extraction with hexane, and the sample with middle fat was further purified using solid phase extraction with a C8 column. The condiment sample with high fat was diluted with hexane, then dissolved with saturated NaCl solution (adjusting pH to 2 -3 with phosphoric acid), and extracted with acetonitrile. The extract was purified using solid phase extraction with a C8 column. The analysis was performed on a C18 column (150 mm x 2.1 mm, 1.7 microm) using a gradient elution with the mobile phases of 20 mmol/L ammonium acetate buffer and acetonitrile, and determined by tandem mass spectrometry in negtive ESI mode under multiple reaction monitoring (MRM) mode. The method had good linearity (r > or = 0.995 5). The limits of quantification (LOQs) (S/N = 10) of 17 analytes ranged from 0.05 mg/kg to 5 mg/kg. The recoveries ranged from 79.7% to 118% at three spiked levels, the relative standard deviations (RSDs) ranged from 0.57% to 13.1%. The method can be applied for the determination of preservatives and antioxidants in condiments.
authors: Chinese Centre for Disease Control and Prevention, Beijing 100021, China.
authors: Yun
authors: Y
authors: Ling
authors: Xiaogang
authors: X
authors: Chu
authors: Feng
authors: F
authors: Zhang
authors: Qi
authors: Q
authors: Chen
authors: Yunfeng
authors: Y
authors: Zhao
authors: Yongning
authors: Y
authors: Wu
authors: Chenguang
authors: C
authors: Zhang
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003212:Condiments / Q000032:analysis*; D005520:Food Preservatives / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D005520:Food Preservatives