title: Mutually facilitated dispersal between the nonmotile fungus Aspergillus fumigatus and the swarming bacterium Paenibacillus vortex.
abstract: In the heterogeneous environment surrounding plant roots (the rhizosphere), microorganisms both compete and cooperate. Here, we show that two very different inhabitants of the rhizosphere, the nonmotile fungus Aspergillus fumigatus and the swarming bacterium Paenibacillus vortex, can facilitate each other's dispersal. A. fumigatus conidia (nonmotile asexual fungal spores) can be transported by P. vortex swarms over distances of at least 30 cm and at rates of up to 10.8 mm h(-1). Moreover, conidia can be rescued and transported by P. vortex from niches of adverse growth conditions. Potential benefit to the bacteria may be in crossing otherwise impenetrable barriers in the soil: fungal mycelia seem to act as bridges to allow P. vortex to cross air gaps in agar plates. Transport of conidia was inhibited by proteolytic treatment of conidia or the addition of purified P. vortex flagella, suggesting specific contacts between flagella and proteins on the conidial surface. Conidia were transported by P. vortex into locations where antibiotics inhibited bacteria growth, and therefore, growth and sporulation of A. fumigatus were not limited by bacterial competition. Conidia from other fungi, similar in size to those fungi from A. fumigatus, were not transported as efficiently by P. vortex. Conidia from a range of fungi were not transported by another closely related rhizosphere bacterium, Paenibacillus polymyxa, or the more distantly related Proteus mirabilis, despite both being efficient swarmers.
authors: Laboratory of Microbiology, Wageningen University, 6703 HB, Wageningen, The Netherlands. colinutrecht@gmail.com
authors: Colin J
authors: CJ
authors: Ingham
authors: Oren
authors: O
authors: Kalisman
authors: Alin
authors: A
authors: Finkelshtein
authors: Eshel
authors: E
authors: Ben-Jacob
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D008124:Locomotion / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D056507:Paenibacillus / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D058441:Rhizosphere; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents