title: LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang).
abstract: A number of three LC-MS/MS hybrid systems (QTof, TripleTof and QTrap) has been used to profile small metabolites (m/z 100-1000) and to detect the targeted metabolites such as quassinoids, alkaloids, triterpene and biphenylneolignans from the aqueous extracts of Eurycoma longifolia. The metabolite profiles of small molecules showed four significant clusters in the principle component analysis for the aqueous extracts of E. longifolia, which had been collected from different geographical terrains (Perak and Pahang) and processed at different extraction temperatures (35°C and 100°C). A small peptide of leucine (m/z 679) and a new hydroxyl methyl β-carboline propionic acid have been identified to differentiate E. longifolia extracts that prepared at 35°C and 100°C, respectively. From the targeted metabolites identification, it was found that 3,4ɛ-dihydroeurycomanone (quassinoids) and eurylene (squalene-type triterpene) could only be detected in the Pahang extract, whereas canthin-6-one-3N-oxide could only be detected in the Perak extract. Overall, quassinoids were present in the highest concentration, particularly eurycomanone and its derivatives compared to the other groups of metabolites. However, the concentration of canthin-6-one and β-carboline alkaloids was significantly increased when the roots of the plant samples were extracted at 100°C.
authors: Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia. lschua@ibd.utm.my
authors: Lee Suan
authors: LS
authors: Chua
authors: Nor Amaiza Mohd
authors: NA
authors: Amin
authors: Jason Chun Hong
authors: JC
authors: Neo
authors: Ting Hun
authors: TH
authors: Lee
authors: Chew Tin
authors: CT
authors: Lee
authors: Mohamad Roji
authors: MR
authors: Sarmidi
authors: Ramlan Abdul
authors: RA
authors: Aziz
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D035701:Eurycoma / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D017705:Lignans / Q000032:analysis* / Q000737:chemistry; D008296:Malaysia; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D025341:Principal Component Analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D000470:Alkaloids; D017705:Lignans; D010936:Plant Extracts; D014315:Triterpenes