title: Over-expression of pcvA involved in vesicle-vacuolar fusion affects the conidiation and penicillin production in Penicillium chrysogenum.
abstract: Rab GTPase is required for vesicle-vacuolar fusion during the vacuolar biogenesis in fungi. Rab GTPase-encoding gene, pcvA, was cloned from Penicillium chrysogenum: it contained five introns and its predicted protein contained the conserved Rab GTPase domain involved in GTP-binding and hydrolysis. Over-expression of pcvA significantly stimulated the vesicle-vacuolar fusion but repressed the conidiation and decreased conidial tolerance against thermal stress. Penicillin production was decreased in the pcvA over-expressed strain suggesting that pcvA is involved in vesicle-vacuolar fusion participates in the penicillin biosynthesis in P. chrysogenum.
authors: The Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors: Xinxin
authors: X
authors: Xu
authors: Jing
authors: J
authors: Yang
authors: Yang
authors: Y
authors: An
authors: Yuanyuan
authors: Y
authors: Pan
authors: Gang
authors: G
authors: Liu
mesh_terms: D003001:Cloning, Molecular; D022162:Cytoplasmic Vesicles / Q000378:metabolism*; D015870:Gene Expression; D025301:Hyphae / Q000254:growth & development*; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014617:Vacuoles / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010406:Penicillins; D020691:rab GTP-Binding Proteins