title: Novel microsphere-based method for detection and typing of 46 mucosal human papillomavirus types.
abstract: We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.
authors: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors: Vanessa
authors: V
authors: Zubach
authors: Gerry
authors: G
authors: Smart
authors: Samuel
authors: S
authors: Ratnam
authors: Alberto
authors: A
authors: Severini
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D008863:Microspheres; D058889:Molecular Typing / Q000379:methods*; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification* / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral