title: Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheep.
abstract: Bluetongue disease has been causing variable morbidity and mortality in sheep in India and other countries of the world. The experimental infection with Indian BTV-1 strain induced mild to sub-acute infection in native sheep. There were low induction of IL-12, IFN-γ and TNF-α cytokine mRNA expressions determined by real time RT-PCR in draining lymph nodes (DLN), spleen, and PBMCs during the initial stages of infection (8 days post inoculation, DPI) and higher around 15 DPI. The reduced pro-inflammatory cytokine (IFN-γ and TNF-α) responses during the initial stage of infection (8 DPI) was also accompanied by similarly decreased T-cell populations and overt clinical symptoms. Later up regulation of these cytokines and substantial increase in the proportion of CD8(+) T-cells occurred with reduction of clinical signs and disappearance of BTV-1 antigen from tissues as determined by immunohistochemistry and RT-PCR. Thus there is definite involvement of pro-inflammatory cytokines and CD8(+) T cell activity in disease induced by BTV-1 strain.
authors: Pathology Laboratory, Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar 243 122, Bareilly, UP, India. rudragoudavet@gmail.com
authors: R
authors: R
authors: Channappanavar
authors: K P
authors: KP
authors: Singh
authors: R
authors: R
authors: Singh
authors: C S
authors: CS
authors: Umeshappa
authors: S L
authors: SL
authors: Ingale
authors: A B
authors: AB
authors: Pandey
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000276:immunology* / Q000821:virology; D001820:Bluetongue virus / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000276:immunology*; D018664:Interleukin-12 / Q000276:immunology*; D018655:Lymphocyte Count / Q000662:veterinary; D008297:Male; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma