title: Steroid 5α-reductase in adult rat brain after neonatal testosterone administration.
abstract: Testosterone (T) plays an important role in developing brain, dictating sex-specific behavior and physiology. 3α,5α-Reduced neurosteroids also regulate reproductive behavior. The key enzyme in the biosynthesis of these neurosteroids is 5α-reductase (5α-R), expressed as two isozymes, 5α-R1 and 5α-R2. In this study, T and sesame oil (vehicle) were administered during postnatal sexual differentiation of the central nervous system (CNS) and mRNA levels of 5α-R isozymes, were measured using quantitative RT-PCR in prefrontal cortex of male and female rats with different androgenic status at adulthood. Our results indicate that T concentrations during postnatal sexual differentiation of the rat CNS, among other sex-dependent factors, influence brain levels of 5α-R isozymes in adulthood and the pattern of their regulation by androgen hormones.
authors: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Granada, Spain.
authors: Pilar
authors: P
authors: Sánchez
authors: Jesús M
authors: JM
authors: Torres
authors: Raimundo G
authors: RG
authors: del Moral
authors: Juan
authors: J
authors: de Dios Luna
authors: Esperanza
authors: E
authors: Ortega
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D017397:Prefrontal Cortex / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D013739:Testosterone / Q000494:pharmacology / Q000502:physiology*
chemical_list: D007527:Isoenzymes; D013739:Testosterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase