title: Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry.
abstract: Reaction of reactive nitrogen species (RNS), such as peroxynitrite and nitryl chloride with soluble tyrosine and tyrosine residues in proteins produces soluble 3-nitro-tyrosine and 3-nitro-tyrosino-proteins, respectively. Regular proteolysis of 3-nitro-tyrosino-proteins yields soluble 3-nitro-tyrosine. 3-Nitro-tyrosine circulates in plasma and is excreted in the urine. Both circulating and excretory 3-nitro-tyrosine are considered suitable biomarkers of nitrative stress. Tandem mass spectrometry coupled with gas chromatography (GC-MS/MS) or liquid chromatography (LC-MS/MS) is one of the most reliable analytical techniques to determine 3-nitro-tyrosine. Here, we describe protocols for the quantitative determination of soluble 3-nitro-tyrosine in human plasma and urine by GC-MS/MS.
authors: Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany. tsikas.dimitros@mh-hannover.de
authors: Dimitrios
authors: D
authors: Tsikas
authors: Anja
authors: A
authors: Mitschke
authors: Frank-Mathias
authors: FM
authors: Gutzki
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009573:Nitrites / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000737:chemistry; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine
chemical_list: D009573:Nitrites; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine; C445660:nitryl chloride