title: Extraction of nucleic acids from bone.
abstract: Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.
authors: Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK. a.hughes@abdn.ac.uk
authors: Alun
authors: A
authors: Hughes
authors: Tracy L
authors: TL
authors: Stewart
authors: Val
authors: V
authors: Mann
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry* / Q000166:cytology*; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D013053:Spectrophotometry
chemical_list: D012313:RNA; D004247:DNA