title: In vitro photodynamic inactivation of Candida spp. by different doses of low power laser light.
abstract: This study evaluated the efficacy of PDT in photoinactivation of Candida species using methylene blue (MB) and irradiation with a diode laser (660nm, 40mW). Suspensions of Candida species were obtained containing 10(6)cfu/ml, transferred to 96-holes plates and exposed to 03 doses of laser light (60J/cm(2), 120J/cm(2), 180J/cm(2)) in the presence of MB. Additional suspensions were treated with only the MB, the laser light or with 0.85% saline (control groups). After the treatments, 1μl aliquot of the suspensions was plated in duplicate on SDA. The plates were incubated at 37°C for 24-48h and after this period there was the counting of colonies (cfu/ml). The three evaluated doses determined meaningful inactivation of Candida spp. (p<0.05). The 180J/cm(2) dose was the most effective, inactivating 78% of cfu/ml. At a dose of 180J/cm(2)C. albicans was the most susceptible specie. PDT has demonstrated effectiveness in the inactivation of Candida spp.
authors: Department of Social and Clinical Dentistry, School of Dentistry, Federal University of Paraíba, UFPB, PB, Brazil. desasq@hotmail.com
authors: A S
authors: AS
authors: Queiroga
authors: V N
authors: VN
authors: Trajano
authors: E O
authors: EO
authors: Lima
authors: A F M
authors: AF
authors: Ferreira
authors: A S
authors: AS
authors: Queiroga
authors: F A
authors: FA
authors: Limeira
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002175:Candida / Q000502:physiology* / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D008027:Light; D010778:Photochemotherapy / Q000379:methods; D011829:Radiation Dosage; D013242:Sterilization / Q000379:methods*