title: The effectiveness of magnesium oxide combined with tissue conditioners in inhibiting the growth of Candida albicans: an in vitro study.
abstract: BACKGROUND
  The presence of Candida albicans on the fitting surface of the denture is a major causative factor in denture stomatitis. A treatment method is by combining tissue conditioner and antifungal agents.
  
  
  OBJECTIVE
  The main objective of this study is to test the efficacy of magnesium oxide combined with two tissue conditioners (Viscogel and GC Soft), in inhibiting the growth of Candida albicans.
  
  
  METHODS
  Microbiological study was done in the Department of Microbiology, K S Hegde Medical Academy, Nitte University, Mangalore.
  
  
  METHODS
  A total of 154 plates were prepared using Muller Hilton with Glucose and Methylene Blue dye medium and inoculated with 24-hr old standard Candida culture. Plates were divided into control and combination. Test discs with different concentrations of MgO were equidistantly placed in MgO Control, while sterile discs embedded with respective tissue conditioner were equidistantly placed in Viscogel and GC Soft controls. For combination groups, the tissue conditioners were mixed and the discs with MgO (1%, 3%, 5%, and 7%) were embedded in the mix. After 24 h of incubation, inhibition diameters were noted.
  
  
  METHODS
  The data was analysed using Mann Whitney U Test, ANOVA, Tukey HSD test. Results : The inhibition effect of magnesium oxide 1% combined with tissue conditioners (VGC and GCC) is not significant in both the groups. The inhibition effect of MgO 5% and 7% combined with tissue conditioners (VGC and GCC) is very highly significant ( P < 0.001).
  
  
  CONCLUSIONS
  Magnesium oxide in combination with tissue conditioners are effective against Candida albicans; GC soft with magnesium oxide showed a better result than Viscogel with magnesium oxide; Increasing the concentration of magnesium oxide increases the zone of inhibition of Candida albicans.
authors: Department of Prosthodontics and Crown and Bridge, A. B Shetty Memorial Institute of Dental Sciences, Mangalore, Karnataka, India. hemak_19@yahoo.com
authors: Hema
authors: H
authors: Kanathila
authors: Aruna M
authors: AM
authors: Bhat
authors: Prasad D
authors: PD
authors: Krishna
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004396:Coloring Agents; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008277:Magnesium Oxide / Q000008:administration & dosage / Q000494:pharmacology*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008751:Methylene Blue; D008768:Methylmethacrylates / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010795:Phthalic Acids / Q000737:chemistry; D013997:Time Factors; D014016:Tissue Conditioning, Dental / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D001672:Biocompatible Materials; D004396:Coloring Agents; D003470:Culture Media; D008689:Methacrylates; D008768:Methylmethacrylates; D010795:Phthalic Acids; C073909:GC Soft-Liner; D008277:Magnesium Oxide; C009931:poly(ethylmethacrylate); D008751:Methylene Blue