title: Serum metabonomics study of adenine-induced chronic renal failure in rats by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
abstract: An ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF MS) metabonomics approach was employed to study the serum metabolic profiling of adenine-induced chronic renal failure (CRF) rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the CRF and the normal control groups. Potential biomarkers were screened by using S-plot and were identified by the accurate mass, isotopic pattern and MS/MS fragments information obtained from UPLC Q-TOF MS analysis. Significant differences in the serum level of creatinine, amino acids and LysoPCs were observed, indicating the perturbations of amino acid metabolism and phospholipid metabolism in adenine-induced CRF rats. This research proved that metabonomics is a promising tool for disease research.
authors: Department of Traditional Chinese Medicine, the College of Life Sciences, Northwest University, Shaanxi, China. zyy@nwu.edu.cn
authors: Ying-Yong
authors: YY
authors: Zhao
authors: Xian-Long
authors: XL
authors: Cheng
authors: Feng
authors: F
authors: Wei
authors: Xin-Yue
authors: XY
authors: Xiao
authors: Wen-Ji
authors: WJ
authors: Sun
authors: Yongmin
authors: Y
authors: Zhang
authors: Rui-Chao
authors: RC
authors: Lin
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D003404:Creatinine / Q000097:blood* / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000139:chemically induced; D008246:Lysophospholipids / Q000097:blood* / Q000378:metabolism; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014364:Tryptophan / Q000097:blood* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008246:Lysophospholipids; D014364:Tryptophan; D003404:Creatinine; D000225:Adenine