title: Establishment of bipotent progenitor cell clone from rat skeletal muscle.
abstract: The present study describes the isolation, cloning and characterization of adipogenic progenitor cells from rat skeletal muscle. Among the obtained 10 clones, the most highly adipogenic progenitor, 2G11 cells, were further characterized. In addition to their adipogenicity, 2G11 cells retain myogenic potential as revealed by formation of multinucleated myotubes when co-cultured with myoblasts. 2G11 cells were resistant to an inhibitory effect of basic fibroblast growth factor on adipogenesis, while adipogenesis of widely used preadipogenic cell line, 3T3-L1 cells, was suppressed almost completely by the same treatment. In vivo transplantation experiments revealed that 2G11 cells are able to possess both adipogenicity and myogenicity in vivo. These results indicate the presence of bipotent progenitor cells in rat skeletal muscle, and suggest that such cells may contribute to ectopic fat formation in skeletal muscle.
authors: Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan.
authors: Yousuke
authors: Y
authors: Murakami
authors: Erica
authors: E
authors: Yada
authors: Shin-ichi
authors: S
authors: Nakano
authors: Yuko
authors: Y
authors: Miyagoe-Suzuki
authors: Tohru
authors: T
authors: Hosoyama
authors: Takashi
authors: T
authors: Matsuwaki
authors: Keitaro
authors: K
authors: Yamanouchi
authors: Masugi
authors: M
authors: Nishihara
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000502:physiology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D008297:Male; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000502:physiology
chemical_list: D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors