title: Protective effects of paeoniflorin against corticosterone-induced neurotoxicity in PC12 cells.
abstract: Neuroprotection has been proposed as one of the acting mechanisms of antidepressants. Paeoniflorin, a monoterpene glycoside, has been reported to display antidepressant-like effects in animal models of behavioural despair. The present study aimed to examine the protective effect of paeoniflorin treatment on corticosterone-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. Paeoniflorin was shown to elevate cell viability, decrease levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) in corticosterone-treated PC12 cells. Paeoniflorin also reversed the reduced nerve growth factor (NGF) mRNA level caused by corticosterone in PC12 cells. The results suggest that paeoniflorin exerts a neuroprotective effect on corticosterone-induced neurotoxicity in PC12 cells, at least in part, via the inhibition of oxidative stress and the up-regulation of NGF expression. This neuroprotective effect may be one of the action pathways that accounts for the in vivo antidepressant activity of paeoniflorin.
authors: College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou, 310053 Zhejiang, China.
authors: Qing-Qiu
authors: QQ
authors: Mao
authors: Xiao-Ming
authors: XM
authors: Zhong
authors: Feng-Mei
authors: FM
authors: Qiu
authors: Zhao-Yi
authors: ZY
authors: Li
authors: Zhen
authors: Z
authors: Huang
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003345:Corticosterone / Q000009:adverse effects; D005960:Glucosides / Q000494:pharmacology*; D008315:Malondialdehyde / Q000032:analysis; D039821:Monoterpenes; D020932:Nerve Growth Factor / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis; D015854:Up-Regulation / Q000187:drug effects; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D003345:Corticosterone; D005960:Glucosides; D008315:Malondialdehyde; D039821:Monoterpenes; D020932:Nerve Growth Factor; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C015423:peoniflorin; D006027:Glycosides