title: Virulence factors and TEM-type β-lactamases produced by two isolates of an epidemic Klebsiella pneumoniae strain.
abstract: Two Klebsiella pneumoniae isolates of the same strain, identified in Poland, produced either TEM-47 or TEM-68, which differed by the Arg275Leu substitution. They harbored a few virulence factors, including an iron-chelating factor and capsule overproduction, suggesting that these factors were sufficient to enhance their nosocomial potency. TEM-68 and TEM-47 had similar enzymatic activities, but TEM-68 was less susceptible to inhibitors than TEM-47. These results confirm the role of the Arg275Leu substitution in the evolution of TEM enzymes.
authors: CHU Clermont-Ferrand, Centre de Biologie, Laboratoire de Bactériologie Clinique, Clermont-Ferrand, France. frobin@chu-clermontferrand.fr
authors: Frédéric
authors: F
authors: Robin
authors: Claire
authors: C
authors: Hennequin
authors: Marek
authors: M
authors: Gniadkowski
authors: Racha
authors: R
authors: Beyrouthy
authors: Joanna
authors: J
authors: Empel
authors: Lucie
authors: L
authors: Gibold
authors: Richard
authors: R
authors: Bonnet
mesh_terms: D019943:Amino Acid Substitution; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D058872:Epidemics; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011044:Poland / Q000453:epidemiology; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis* / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D037521:Virulence Factors; C117192:beta-lactamase TEM-47; C410344:beta-lactamase TEM-68; D001618:beta-Lactamases