title: Biomimetic CO₂ sequestration using purified carbonic anhydrase from indigenous bacterial strains immobilized on biopolymeric materials.
abstract: The present study deals with immobilization of purified CA and whole cell of Pseudomonas fragi, Micrococcus lylae, and Micrococcus luteus 2 on different biopolymer matrices. Highest enzyme immobilization was achieved with P. fragi CA (89%) on chitosan-KOH beads, while maximum cell immobilization was achieved with M. lylae (75%) on chitosan-NH(4)OH beads. A maximum increase of 1.08-1.18 fold stability between 35 and 55°C was observed for M. lylae immobilized CA. The storage stability was improved by 2.02 folds after immobilization. FTIR spectra confirmed the adsorption of CA on chitosan-KOH beads following hydrophilic interactions. Calcium carbonate precipitation was achieved using chitosan-KOH immobilized P. fragi CA. More than 2 fold increase in sequestration potential was observed for immobilized system as compared to free enzyme. XRD spectra revealed calcite as the dominant phase in biomimetically produced calcium carbonate.
authors: Bacteriology Laboratory, Department of P.G. Studies and Research in Biological Science, Rani Durgavati University, Pachpedi, Jabalpur 482001, Madhya Pradesh, India. anjoo1999@gmail.com
authors: Anjana
authors: A
authors: Sharma
authors: Abhishek
authors: A
authors: Bhattacharya
authors: Ankita
authors: A
authors: Shrivastava
mesh_terms: D000464:Alginates / Q000737:chemistry; D040761:Biomimetic Materials / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002119:Calcium Carbonate / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018914:Cells, Immobilized / Q000201:enzymology*; D048271:Chitosan / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008837:Micrococcus / Q000201:enzymology; D016982:Micrococcus luteus / Q000201:enzymology; D044206:Pseudomonas fragi / Q000201:enzymology*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D002245:Carbon Dioxide; D020723:Glucuronic Acid; D048271:Chitosan; D002256:Carbonic Anhydrases; D002119:Calcium Carbonate