title: Increased expression of calponin-3 in epileptic patients and experimental rats.
abstract: Calponin-3 is an actin-interacting protein and is expressed in the brain. Our previous microarray scan has found an up-regulation of calponin-3 gene CNN3 in the temporal lobe of patients with drug-resistant epilepsy. Here we investigated in epileptic patients the changes of brain and cerebrospinal fluid (CSF) calponin-3 expressions, and assessed calponin-3 expression pattern in a rat model of pilocarpine-induced epilepsy. We showed that in the temporal neocortices of 30 patients with drug-resistant epilepsy, both mRNA and protein level of calponin-3 were significantly increased. In addition, the augmentation of CSF calponin-3 from 126 epileptic patients was closely correlated with disease duration. Moreover, in the cortices of temporal lobes of pilocarpine-treated rats, calponin-3 increased along with the time and maintained at significant high levels for up to 2 months, while the up-regulation of hippocampal calponin-3 only occurred at 24h and 1 week. The elevated calponin-3 suggests that deregulation of actin filament dynamics in axonal and dendritic outgrowth and synaptic rearrangement may contribute to pathophysiology of epilepsy.
authors: Department of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, PR China.
authors: Yanbing
authors: Y
authors: Han
authors: Huan
authors: H
authors: Yin
authors: Yali
authors: Y
authors: Xu
authors: Qiong
authors: Q
authors: Zhu
authors: Jing
authors: J
authors: Luo
authors: Xuefeng
authors: X
authors: Wang
authors: Guojun
authors: G
authors: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002135:Calcium-Binding Proteins / Q000134:cerebrospinal fluid* / Q000235:genetics; D004195:Disease Models, Animal; D004569:Electroencephalography; D004827:Epilepsy / Q000134:cerebrospinal fluid* / Q000139:chemically induced / Q000473:pathology*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008840:Microfilament Proteins / Q000134:cerebrospinal fluid* / Q000235:genetics; D008875:Middle Aged; D010862:Pilocarpine / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012189:Retrospective Studies; D018709:Statistics, Nonparametric; D013997:Time Factors; D055815:Young Adult; D000096985:Calponins
chemical_list: D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D010862:Pilocarpine; D012333:RNA, Messenger; D000096985:Calponins