title: Matrix metalloproteinase activity in stifle synovial fluid of cranial cruciate ligament deficient dogs and effect of postoperative doxycycline treatment.
abstract: This prospective clinical study investigated the activity of matrix metalloproteinases (MMPs) in stifle synovial fluid (SF) of 13 dogs with acute cranial cruciate ligament (CCL) rupture, and the effect of a postoperative doxycycline treatment. MMP-2, 3, 9 and 13 activities were compared with respect to the time of sampling (preoperatively or 1 month after surgical stabilisation) and the type of postoperative adjuvant treatment (doxycycline or not). No significant activity was detected for both MMP-3 and MMP-13. MMP-2 and MMP-9 activities were found to be significantly highly increased in SF of CCL ruptured stifles compared to control stifles of unaffected dogs. No significant effect from surgical stabilisation and postoperative doxycycline treatment on MMP-2 and MMP-9 activities was found, indicating that doxycycline may not be an appropriate postoperative medical treatment after CCL rupture.
authors: Department of Small Animal Surgery, ONIRIS Nantes-Atlantic College of Veterinary Medicine, Food Science and Engineering, Atlanpole-La Chantrerie, BP 40706, 44307 Nantes Cedex 3, France.
authors: M
authors: M
authors: Rabillard
authors: R
authors: R
authors: Danger
authors: I P
authors: IP
authors: Doran
authors: G W
authors: GW
authors: Niebauer
authors: S
authors: S
authors: Brouard
authors: O
authors: O
authors: Gauthier
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery*; D000070598:Anterior Cruciate Ligament Injuries; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004285:Dogs / Q000293:injuries / Q000378:metabolism* / Q000601:surgery; D004318:Doxycycline / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D011184:Postoperative Period; D011446:Prospective Studies; D013264:Stifle / Q000201:enzymology*; D013582:Synovial Fluid / Q000201:enzymology*
chemical_list: D000900:Anti-Bacterial Agents; D020782:Matrix Metalloproteinases; D004318:Doxycycline