title: Maternal inhalation of surface-coated nanosized titanium dioxide (UV-Titan) in C57BL/6 mice: effects in prenatally exposed offspring on hepatic DNA damage and gene expression.
abstract: We investigated effects of maternal pulmonary exposure to titanium dioxide (UV-Titan) on prenatally exposed offspring. Time-mated mice (C57BL/6BomTac) were inhalation exposed (1 h/day to 42 mg UV-Titan/m(3) aerosolised powder or filtered air) during gestation days (GDs) 8-18. We evaluated DNA strand breaks using the comet assay in bronchoalveolar lavage (BAL) cells and livers of the time-mated mice (5 and 26-27 days after inhalation exposure), and in livers of the offspring (post-natal days (PND) 2 and 22). We also analysed hepatic gene expression in newborns using DNA microarrays. UV-Titan exposure did not induce DNA strand breaks in time-mated mice or their offspring. Transcriptional profiling of newborn livers revealed changes in the gene expression related to the retinoic acid signalling pathway in the females, while gene expression in male offspring was unaffected. Changes may be a secondary response to maternal inflammation although no direct link was evident through gene expression analysis.
authors: National Research Centre for the Working Environment, DK- 2100 Copenhagen Ø, Denmark.
authors: Petra
authors: P
authors: Jackson
authors: Sabina
authors: S
authors: Halappanavar
authors: Karin Sørig
authors: KS
authors: Hougaard
authors: Andrew
authors: A
authors: Williams
authors: Anne Mette
authors: AM
authors: Madsen
authors: Jacob Stuart
authors: JS
authors: Lamson
authors: Ole
authors: O
authors: Andersen
authors: Carole
authors: C
authors: Yauk
authors: Håkan
authors: H
authors: Wallin
authors: Ulla
authors: U
authors: Vogel
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D015870:Gene Expression; D019570:Inhalation Exposure; D008099:Liver / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: C009495:titanium dioxide; D014025:Titanium