title: Microsporidial keratitis in patients with hot springs exposure.
abstract: This retrospective study included 10 eyes of 9 patients diagnosed with microsporidial keratitis. All of them were known to contract this disease after taking baths in hot springs. The disease was diagnosed based on detecting microsporidia in corneal scrapings using Gram stain and the modified Kinyoun's acid-fast stain. The specimens from the last six patients were subjected to PCR and then sequencing. All of them revealed that the microorganism identified has a high similarity to Vittaforma corneae. Repeated debridement of the epithelial lesions successfully eradicated the microsporidial infection in all nine patients.
authors: Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
authors: Nai-Wen
authors: NW
authors: Fan
authors: Chih-Chiau
authors: CC
authors: Wu
authors: Te-Li
authors: TL
authors: Chen
authors: Wei-Kuang
authors: WK
authors: Yu
authors: Chien-Pei
authors: CP
authors: Chen
authors: Shui-Mei
authors: SM
authors: Lee
authors: Pei-Yu
authors: PY
authors: Lin
mesh_terms: D000328:Adult; D000368:Aged; D019411:Clinical Laboratory Techniques / Q000379:methods; D003315:Cornea / Q000473:pathology; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005260:Female; D045482:Hot Springs; D006801:Humans; D007634:Keratitis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D008297:Male; D008853:Microscopy; D016881:Microsporidiosis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D021863:Vittaforma / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal