title: Platinum complexes for multi-parametric assays using microarray systems.
abstract: Here, we present a two novel fluorescent dyes ethylenediaminechlorocholylglycinateplatinum(II), [Pt(CG)Cl(en)] complex 1 and bisursodeoxycholate(ethylenediamine)platinum(II), [Pt(UDC)(2)(en)] complex 2 based on well-known cis-platin chemistry. These platinum complexes contain cholylglycinate (CG) and ursodeoxycholate (UDC) as ligands. These compounds enable qualitative detection of double-helix DNA and quantitative detection (from pg to μg). These novel compounds have absorption and emission spectra in a difference range as the common ones (for example: cyanine dyes such as Cy3, Cy5, Cy7,…); therefore, it could allow the multi-parametric detection of DNA arrays, incrementing the capacity of experimental performance per one single array. As a consequence, it will increase the amount of data info obtained per chip. The combination of the intrinsic property of this compounds with the optical properties in different fluorescence channels, can allow introducing a new molecule with a wide range of possible applications in DNA arrays.
authors: Centro de Investigación del Cáncer/IBMCC (USAL/CSIC), Departamento de Medicina and Servicio General de Citometría, University of Salamanca, Salamanca, Spain.
authors: María
authors: M
authors: González
authors: Raquel
authors: R
authors: Bartolomé
authors: Sergio
authors: S
authors: Matarraz
authors: Emilio
authors: E
authors: Rodríguez-Fernández
authors: Juan L
authors: JL
authors: Manzano
authors: Martín
authors: M
authors: Pérez-Andrés
authors: Alberto
authors: A
authors: Orfao
authors: Manuel
authors: M
authors: Fuentes
authors: Julio J
authors: JJ
authors: Criado
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005029:Ethylenediamines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods
chemical_list: D005029:Ethylenediamines; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D009944:Organoplatinum Compounds; D010984:Platinum; C031234:ethylenediamine; D004247:DNA