title: The effect of miR-7 on behavior and global protein expression in glioma cell lines.
abstract: Malignant glioma is a common cancer of the nervous system. Despite recent research efforts in cancer therapy, the prognosis of patients with malignant glioma has remained dismal. MicroRNAs are noncoding RNAs that inhibit the expression of their targets in a sequence-specific manner, and a few have been shown to act as oncogenes or tumor suppressors. Here, we aimed at exploring the precise biological role of microRNA-7 (miR-7) and the global protein changes in glioma cell lines transiently transfected with miR-7. Transfection of miR-7 into glioma cell lines causes inhibition of cell migration and invasion and suppression of tumorigenesis. Moreover, ectopic expression of miR-7 inhibits lung metastases of glioma in vivo. Among 65 protein spots with differential expression separated by 2-DE, 37 proteins were successfully identified by MS/MS analysis. Of those, the 25 downregulated proteins, which include 14-3-3ζ, eukaryotic translation initiation factor 5A (EIF5A), and annexin A4, may be downstream targets of miR-7, a finding that could elucidate some aspects of the behavior of glioma cells at the protein level. In conclusion, the absence of miR-7 function could cause downstream molecules to switch on or off, resulting in glioma development, invasion, and metastases. MiR-7-based gene treatment may be a novel anti-invasion therapeutic strategy for malignant glioma.
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, P R China.
authors: Ze Jun
authors: ZJ
authors: Lu
authors: Sheng Yong
authors: SY
authors: Liu
authors: Yu Qin
authors: YQ
authors: Yao
authors: Yong Jun
authors: YJ
authors: Zhou
authors: Shuang
authors: S
authors: Zhang
authors: Lei
authors: L
authors: Dai
authors: Hong Wei
authors: HW
authors: Tian
authors: Yan
authors: Y
authors: Zhou
authors: Hong Xin
authors: HX
authors: Deng
authors: Jin Liang
authors: JL
authors: Yang
authors: Feng
authors: F
authors: Luo
mesh_terms: D048948:14-3-3 Proteins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D002465:Cell Movement / Q000187:drug effects*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005910:Glioma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010448:Peptide Initiation Factors / Q000096:biosynthesis / Q000235:genetics; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015203:Reproducibility of Results; D014162:Transfection; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D048948:14-3-3 Proteins; D035683:MicroRNAs; D010448:Peptide Initiation Factors; D020543:Proteome; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C549242:MIRN7-1 microRNA, human