title: Evaluation of the brain and kidney renin-angiotensin system and oxidative stress in neonatal handled rats.
abstract: The aim of this study was to test the hypothesis that the renin-angiotensin system (RAS) components, as well as the oxidative stress system, would respond to early environmental changes. Thus, we have evaluated the effects of neonatal handling on both brain and kidney RAS and oxidative stress. Pups were divided into two groups: nonhandled and handled. The procedure consisted of handling them for 1 min/day in the first 10 days of life. On days 1, 5, and 10, animals were killed by decapitation. Blood samples were collected and the brain and kidneys were removed. Renin, AT(1), and AT(2) mRNA expression were evaluated through RT-PCR. Angiotensin II (ANG II) serum concentration was also measured. An increased ANG II concentration, brain and kidney AT(2) mRNA expression were demonstrated. The kidney mRNA AT(1) expression was decreased. There was also a kidney lipid peroxidation increase and a brain superoxide dismutase and catalase decrease. In conclusion, handling in the neonatal period induces the activation of the angiotensinergic system, as well as modulates its mRNA receptor expression. The oxidative stress balance system seems not to be involved.
authors: Faculdade de Biociências e Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre/RS, Brazil.
authors: Daniela L
authors: DL
authors: Rodriguez
authors: Fernanda C
authors: FC
authors: de Mesquita
authors: Débora
authors: D
authors: Attolini
authors: Bruna S
authors: BS
authors: de Borba
authors: Patrícia S
authors: PS
authors: Scherer
authors: Priscilla H
authors: PH
authors: Almeida
authors: Vinícius L
authors: VL
authors: da Costa
authors: Bárbara S
authors: BS
authors: Scherer
authors: Virgínia M
authors: VM
authors: Schmitt
authors: Jarbas R
authors: JR
authors: de Oliveira
authors: Márcio V F
authors: MV
authors: Donadio
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005260:Female; D006234:Handling, Psychological; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000378:metabolism; D012083:Renin / Q000378:metabolism; D012084:Renin-Angiotensin System / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D012083:Renin