title: Enzymatic pretreatment for preparing starch nanocrystals.
abstract: Starch nanocrystals (SNCs) are crystalline platelets resulting from the acid hydrolysis of starch. A limiting factor for their more widespread use is their preparation duration. Therefore, this study investigates the possibility of developing an enzymatic pretreatment of starch to reduce the acid hydrolysis duration. A screening of three types of enzymes, namely, α-amylase, β-amylase, and glucoamylase, is proposed, and the latter was selected for a pretreatment. Compared with the regular kinetics of hydrolysis for preparing SNC, that of pretreated starch was much faster. The extent of hydrolysis normally reached in 24 h was obtained after only 6 h, and the regular final yield (15% after 5 days) was reached in 45 h. AFM and X-ray diffraction measurements confirmed that the obtained nanoparticles were indeed SNC.
authors: LGP2 - Laboratory of Pulp and Paper Sciences, 461 Rue de la Papeterie, BP 65, 38402 Saint Martin d'Hères Cedex, France.
authors: Déborah
authors: D
authors: LeCorre
authors: Elina
authors: E
authors: Vahanian
authors: Alain
authors: A
authors: Dufresne
authors: Julien
authors: J
authors: Bras
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D001467:Hordeum / Q000201:enzymology; D006868:Hydrolysis; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010179:Pancreas / Q000201:enzymology; D010316:Particle Size; D010940:Plant Proteins / Q000737:chemistry; D013213:Starch / Q000737:chemistry* / Q000648:ultrastructure; D013552:Swine; D013997:Time Factors; D003313:Zea mays / Q000737:chemistry; D000516:alpha-Amylases / Q000737:chemistry*; D001614:beta-Amylase / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D013213:Starch; D000516:alpha-Amylases; D001614:beta-Amylase; D005087:Glucan 1,4-alpha-Glucosidase