title: Mechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis.
abstract: An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F)-glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.
authors: Institute of Organic Chemistry, Julius-Maximilians University Würzburg, Würzburg, Germany.
authors: Julian
authors: J
authors: Görl
authors: Malte
authors: M
authors: Timm
authors: Jürgen
authors: J
authors: Seibel
mesh_terms: D019751:Intramolecular Transferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D020560:Proteobacteria / Q000201:enzymology; D014312:Trisaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D014312:Trisaccharides; C026440:glucosylsucrose; D019751:Intramolecular Transferases; C042002:isomaltulose synthase