title: Recent advances in microfluidic technologies for biochemistry and molecular biologys.
abstract: Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.
authors: Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Switzerland.
authors: Soongwon
authors: S
authors: Cho
authors: Dong-Ku
authors: DK
authors: Kang
authors: Jaebum
authors: J
authors: Choo
authors: Andrew J
authors: AJ
authors: de Mello
authors: Soo-Ik
authors: SI
authors: Chang
mesh_terms: D001671:Biochemistry / Q000639:trends*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods* / Q000639:trends*; D008967:Molecular Biology / Q000639:trends*; D040901:Proteomics