title: pH-Uncontrolled lactic acid fermentation with activated carbon as an adsorbent.
abstract: In this paper, we presented a novel process involving activated carbon (AC) as an adsorbent for lactic acid fermentation, separation and oligomerization. It was found that pH has a significant effect on the adsorption of lactic acid on AC. The use of AC for in situ removal of lactic acid successfully decreased the inhibitory effect of lactic acid, resulting in significant increases in both productivity and yield. Acetone was used to desorb lactic acid and it was confirmed that the acetone treatment did not decrease the optical purity of the lactic acid, i.e., the optical purity was as high as 99.5% after desorption. Due to the presence of little materials influencing lactic acid oligomerization, oligomers with an optical purity of above 96% and a weight-average molecular weight (M(w)) of 2400 were obtained in the oligomerization process.
authors: Biotechnology Laboratory, Toyota Central R&D Labs Inc., 41-1 Nagakute-Yokomichi, Aichi 480-1192, Japan. e0788@mosk.tytlabs.co.jp
authors: Min-Tian
authors: MT
authors: Gao
authors: Takashi
authors: T
authors: Shimamura
authors: Nobuhiro
authors: N
authors: Ishida
authors: Haruo
authors: H
authors: Takahashi
mesh_terms: D000096:Acetone; D000327:Adsorption; D000818:Animals; D002417:Cattle / Q000235:genetics; D002606:Charcoal / Q000737:chemistry*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000080864:Lactate Dehydrogenase 5; D019344:Lactic Acid / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D058105:Polymerization; D011401:Promoter Regions, Genetic; D011767:Pyruvate Decarboxylase / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D012997:Solvents
chemical_list: D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; D000096:Acetone; D002606:Charcoal; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; C101504:PDC1 protein, S cerevisiae; D011767:Pyruvate Decarboxylase