title: Establishment and characterization of outer root sheath (ORS) cell line from Jining grey goat.
abstract: A new line of outer root sheath (ORS) cells was established from hair follicles of Jining grey goat by using a mechanical separation combined with enzyme digestion. Cell morphology is described at different phases. The chromosome analysis of ORS cells, identification of the ORS cells and morphological reversion test were detected at the 4th and 40th passages. The ORS cells were healthy and the growth characteristics were stable with a population doubling time of 52 h. Chromosome analysis showed that >58% of cells were diploid. Test for ORS cell line CK19 expression was positive. This newly established ORS cell line not only lays the foundation for further studying on the growth, regeneration, development law of goat hair follicle but also provides a mirror for the research of human hair in medical field.
authors: Shandong Agricultural University, Daizong Road No. 61, Taian 271018, Shandong, People's Republic of China.
authors: Zhifeng
authors: Z
authors: Cui
authors: Yanxia
authors: Y
authors: Hu
authors: Hui
authors: H
authors: Wang
authors: Yongqing
authors: Y
authors: Zeng
authors: Bin
authors: B
authors: Dong
authors: Houshun
authors: H
authors: Zhu
authors: Zhongdian
authors: Z
authors: Dong
authors: Zhiyuan
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D004171:Diploidy; D006041:Goats; D018859:Hair Follicle / Q000166:cytology*; D007621:Karyotyping; D012692:Serial Passage