title: Rodent osteoclast cultures.
abstract: This chapter describes quantitative methods for isolating and culturing rodent osteoclasts on dentine, a bone-like, resorbable substrate. These techniques generate relatively large numbers of osteoclasts and allow the key processes of osteoclast formation and activation to be studied independently. A special focus will be on the role of extracellular pH, a critical factor in the control of osteoclast function.
authors: Department of Cell and Developmental Biology, University College London, London, UK. i.orriss@ucl.ac.uk
authors: Isabel R
authors: IR
authors: Orriss
authors: Timothy R
authors: TR
authors: Arnett
mesh_terms: D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003804:Dentin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D016707:Tissue Fixation; D054457:Tissue Scaffolds / Q000737:chemistry