title: [Enhanced immune response of a novel T-cell immunogen in vaccine for foot-and-mouth disease].
abstract: We investigated the enhanced immune response of a recombinant T cell immunogen as an effective cellular immune adjuvant. The T cell immunogen named TI contained several T cell epitopes from the VP1, VP4, 3A and 3D proteins of foot-and-mouth disease virus (FMDV) and two pan-T helper (T(H)) cell sites to broaden the immunogenicity of the protein. Meanwhile, another fusion protein named OA-VP1 was expressed in bacteria, which contained two VP1 proteins of O and Asia1 type FMDV. Mice were vaccinated with commercially inactivated vaccine or OA-VP1 protein with or without the TI immunogen. The results show that mice inoculated with inactivated vaccine or OA-VP1 protein supplemented with TI immunogen produced significantly higher level of neutralizing antibodies (P < 0.01 or P < 0.05) than the mice only inoculated with inactivated vaccine or OA-VP1 protein by microneutralization assay. An obvious increase in T cell number by flow cytometric analysis and significantly higher concentration of IFN-gamma secreted in culture media of spleen lymphocytes were observed in groups supplemented with TI immunogen (P < 0.01). TI immunogen was an effective stimulator for humoral and cellular immunity and could help improve the immunogenicity of inactivated vaccine or protein subunit vaccine.
authors: National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors: Qing
authors: Q
authors: Zhao
authors: Pu
authors: P
authors: Sun
authors: Zaixin
authors: Z
authors: Liu
authors: Pinghua
authors: P
authors: Li
authors: Huifang
authors: H
authors: Bao
authors: Yimei
authors: Y
authors: Cao
authors: Xingwen
authors: X
authors: Bai
authors: Yuanfang
authors: Y
authors: Fu
authors: Zengjun
authors: Z
authors: Lu
authors: Dong
authors: D
authors: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D007114:Immunization; D051379:Mice; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000276:Adjuvants, Immunologic; D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines