title: Dried blood spots as a sampling technique for the quantitative determination of guanfacine in clinical studies.
abstract: BACKGROUND
  Dried blood spot (DBS) technology was evaluated for the quantitative determination of guanfacine in human blood in clinical studies. A very sensitive DBS assay has been developed using HPLC coupled with an AB Sciex 5500 QTRAP® (Applied Biosystems/MDS Sciex, ON, Canada) MS system (LC-MS/MS) with a linear calibration range of 0.05 to 25 ng/ml. High-resolution MS using an Exactive Orbitrap® (ThermoFisher, LLC., CA, USA) was compared with the QTRAP using extracted exact mass ion current profiles for guanfacine and its stable-isotope-incorporated internal standard. The sample preparation employed liquid-liquid extraction with methyl t-butyl ether of 5 mm punched DBS card disks, followed by reversed-phase HPLC separation coupled with either MS/MS or high-resolution MS. Routine experiments were performed to establish the robustness of the DBS assay, including precision, accuracy, linearity, selectivity, sensitivity and long-term stability of up to 76 days. In addition, several factors that potentially affect quantitation were investigated, including blood volume for DBS spotting, punch size and punch location.
  
  
  RESULTS
  A sensitive research assay with a LLOQ of 0.05 ng/ml was developed and subjected to several components of a method validation common to a regulated bioanalysis procedure employing DBS. This method development and partial validation study determined that spot volume, punch size or punch location do not affect assay accuracy and precision. The DBS approach was successfully applied to a clinical study (a Phase I, randomized, double-blind, placebo-controlled, crossover study to assess the effect of varying multiple oral doses of guanfacine on the pharmacokinetic, pharmacodynamic, safety, and tolerability profiles in healthy adult subjects). The pharmacokinetic profiles for 12 volunteers generated from the DBS assay and from a previously validated plasma assay were compared and were found to be comparable. DBS incurred samples collected from finger prick blood and directly applied to the DBS cards were also analyzed for comparison.
  
  
  CONCLUSIONS
  From a bioanalytical perspective, DBS sample collection and analysis is a potentially viable alternative for guanfacine determination in clinical studies, utilizing approximately 100 µl of blood per subject profile compared with a few millilitres of blood drawn for conventional plasma bioanalysis.
authors: Advion BioServices, Inc., Ithaca, NY, USA.
authors: Yuanyuan
authors: Y
authors: Li
authors: Jack
authors: J
authors: Henion
authors: Richard
authors: R
authors: Abbott
authors: Phillip
authors: P
authors: Wang
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D002986:Clinical Trials as Topic / Q000379:methods; D059788:Dried Blood Spot Testing / Q000379:methods*; D016316:Guanfacine / Q000097:blood*; D006801:Humans; D059625:Liquid-Liquid Extraction; D008297:Male; D013048:Specimen Handling / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000316:Adrenergic alpha-Agonists; D016316:Guanfacine