title: SNP analysis of minimally evolved t(14;18)(q32;q21)-positive follicular lymphomas reveals a common copy-neutral loss of heterozygosity pattern.
abstract: Follicular lymphoma (FL) cases with a t(14;18)(q32;q21) and minimal or no additional karyotypic alterations, such as copy number gains and losses and/or chromosomal rearrangements, may exhibit pathologic features and a clinical behavior similar to those with more complex karyotypes. This study sought to investigate whether the copy-neutral loss of heterozygosity (cnLOH) profiles of these minimally evolved t(14;18)(q32;q21)-positive follicular lymphoma (MEV-FL) cases are similar to or different from the majority of FL cases with more karyotypic alterations. Affymetrix SNP 6.0 array analysis was applied to the tumor genomes of 23 MEV-FL biopsy samples to assess for the presence of cnLOH. These cases carried either a single or no chromosomal abnormality in addition to t(14;18)(q32;q21) as determined by karyotyping. We found that, although these MEV-FL cases had simple karyotypes, they showed very similar cnLOH profiles as compared to cytogenetically complex cases. The most frequent regions affected by cnLOH were 1p (17%), 6p (17%), 12q (13%) and 16p (13%). Our study suggests that cnLOH alterations may serve as important contributors to the pathological and clinical manifestations of FL.
authors: Center for Lymphoid Cancer, British Columbia Cancer Agency, Vancouver, BC, Canada. kjcheungjr@bccancer.bc.ca
authors: K J
authors: KJ
authors: Cheung
authors: S
authors: S
authors: Rogic
authors: S
authors: S
authors: Ben-Neriah
authors: M
authors: M
authors: Boyle
authors: J M
authors: JM
authors: Connors
authors: R D
authors: RD
authors: Gascoyne
authors: D E
authors: DE
authors: Horsman
mesh_terms: D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D056915:DNA Copy Number Variations; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D059785:Karyotype; D007621:Karyotyping / Q000379:methods; D019656:Loss of Heterozygosity; D008224:Lymphoma, Follicular / Q000235:genetics*; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide