title: Levels of 5-hydroxyindol acetic acid and lipid peroxidation in brain after administration of marijuana and nalbuphine in male and female rat.
abstract: We analyzed the effect of marijuana and nalbuphine on levels of 5-hydroxyindol acetic acid and lipid peroxidation in rat brain. Single and repeated dosages of 250 mg/kg marijuana extract or 10 mg/kg nalbuphine were administered to male and female Wistar rats. Animals were sacrificed and brains were obtained to measure the content of 5-hydroxyindol acetic acid, reduced glutathione, thiobarbituric acid reactive substances, total ATPase and Na+/K+ ATPase activities. There was an increase in thiobarbituric acid reactive substances, total ATPase and Na+/K+ ATPase activity in the animals that received a single dose of marijuana and nalbuphine (p=0.001), with a notable decrease in glutathione and 5-hydroxyindol acetic acid levels (p=0.001). Both marijuana and nalbuphine increased levels of oxidative damage biomarkers in rat brain and decreased glutathione and 5-hydroxyindol acetic acid levels which could provoke changes in cellular and biochemical regulations and serotonergic activity in either male or female rats.
authors: Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), Universidad Nacional Autónoma de Mexico.
authors: David
authors: D
authors: Calderón Guzmán
authors: Norma
authors: N
authors: Labra Ruiz
authors: Ernestina
authors: E
authors: Hernández García
authors: Hugo
authors: H
authors: Juárez Olguín
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002188:Cannabis; D005260:Female; D005978:Glutathione / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D009266:Nalbuphine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006897:Hydroxyindoleacetic Acid; D000251:Adenosine Triphosphatases; D005978:Glutathione; D009266:Nalbuphine