title: An assessment of androgenic/anti-androgenic effects of GH transgenic carp by Hershberger assay.
abstract: OBJECTIVE
  To evaluate the androgenic and anti-androgenic effects of GH (growth hormone) transgenic carp in male rats.
  
  
  METHODS
  Hershberger assay was carried out in castrated male SD rats aged 4-5 weeks. Testosterone propionate (TP) (0.4 mg/kg BW) was administrated for a positive control, GH transgenic carp (3.0 g/kg BW)+TP (0.4 mg/kg BW), parental carp (3.0 g/kg BW) + TP (0.4 mg/kg BW), and flutamide (Flu) (3.0 g/kg BW) were used for negative controls, and vehicle was administered orally for a blank control. All groups were administrated for 10 consecutive days. At the end of the test, animals were anesthetized, then weights of accessory sex organ were measured. Serum testosterone (T), luteinizing hormone (LH), and Follicle-Stimulating Hormone (FSH) levels were detected.
  
  
  RESULTS
  The weights ratios of the accessory sex organs and body weights showed no significant differences between the solvent control and the GH transgenic carp-treated groups. Serum concentrations of FSH, LH, and T of the rats treated with GH transgenic carp + TP showed no significant changes, compared with those treated with TP only.
  
  
  CONCLUSIONS
  GH transgenic carp does not have any androgenic agonist or antagonist properties in vivo screening tests.
authors: Beijing Union University, Beijing, China.
authors: YuMei
authors: Y
authors: Liu
authors: WenZhong
authors: W
authors: Zhang
authors: Ling
authors: L
authors: Yong
authors: XiaoHong
authors: X
authors: Zhao
authors: XuDong
authors: X
authors: Jia
authors: Ning
authors: N
authors: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002347:Carps / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000097:blood; D005837:Genitalia, Male / Q000187:drug effects*; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051381:Rats; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone