title: Oxidative effect of methotrexate administration in spinal cord of rabbits.
abstract: OBJECTIVE
  To study the potential effect of Methotrexate (MTX) on lipid peroxidation and activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) two important endogenous anti-oxidant enzymes.
  
  
  METHODS
  Twenty healthy male Newzland rabbits weighting 1500-1800 g were divided into two groups. One group was considered as the MTX groups and the other was considered as control group. Rabbits had free access to food and tap water. Rabbits in MTX group received a single dose of MTX, 20 mg/kg intraperitoneally, a similar volume of saline was administered to control group. After 6 days rabbits were uteinized and spinal tissue excised for biochemical studies.
  
  
  RESULTS
  GPX and SOD activities in spinal tissue in rabbits of MTX group significantly reduced after MTX administration compared to control group(P = 0.002 vs 0.18). An increase in the tissue MDA level was seen in the MTX group, suggesting increased lipid peroxidation. Levels of MDA were significantly higher in MTX group compared to control group (P = 0.002).
  
  
  CONCLUSIONS
  Our data suggests that MTX treatment induces oxidative tissue damage on the spinal tissue, as assessed by increased lipid peroxidation and decreased GPX and SOD levels, so decreasing oxidative stress by anti-oxidant agents may play a key role in attenuating spinal cord injury.
authors: Neurology Research Center, Islamic Azad University of Tabriz, Tabriz City, Iran.
authors: Hormoz
authors: H
authors: Ayromlou
authors: Babak
authors: B
authors: Hajipour
authors: Mohammad Mehdi
authors: MM
authors: Hossenian
authors: Ali
authors: A
authors: Khodadadi
authors: Amir Mansour
authors: AM
authors: Vatankhah
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008727:Methotrexate / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D011817:Rabbits; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D000975:Antioxidants; D016166:Free Radical Scavengers; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008727:Methotrexate