title: Optically mapping multiple bacterial genomes simultaneously in a single run.
abstract: Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.
authors: Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America.
authors: Matthew C
authors: MC
authors: Riley
authors: James Eric
authors: JE
authors: Lee
authors: Emil
authors: E
authors: Lesho
authors: Benjamin C
authors: BC
authors: Kirkup
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D055095:Optics and Photonics / Q000379:methods*; D012760:Shigella / Q000235:genetics*