title: A new β-glucosidase producing yeast for lower-cost cellulosic ethanol production from xylose-extracted corncob residues by simultaneous saccharification and fermentation.
abstract: This study reports a new yeast strain of Clavispora NRRL Y-50464 that is able to utilize cellobiose as sole source of carbon and produce sufficient native β-glucosidase enzyme activity for cellulosic ethanol production using SSF. In addition, this yeast is tolerant to the major inhibitors derived from lignocellulosic biomass pre-treatment such as 2-furaldehyde (furfural) and 5-(hydroxymethyl)-2-furaldehyde (HMF), and converted furfural into furan methanol in less than 12h and HMF into furan-2,5-dimethanol within 24h in the presence of 15 mM each of furfural and HMF. Using xylose-extracted corncob residue as cellulosic feedstock, an ethanol production of 23 g/l was obtained using 25% solids loading at 37 °C by SSF without addition of exogenous β-glucosidase. Development of this yeast aids renewable biofuels development efforts for economic consolidated SSF bio-processing.
authors: Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL 61604, USA. ZLewis.Liu@ars.usda.gov
authors: Z Lewis
authors: ZL
authors: Liu
authors: Scott A
authors: SA
authors: Weber
authors: Michael A
authors: MA
authors: Cotta
authors: Shi-Zhong
authors: SZ
authors: Li
mesh_terms: D000383:Agriculture / Q000191:economics; D001203:Ascomycota / Q000145:classification / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002681:China; D003362:Cost-Benefit Analysis; D000431:Ethanol / Q000191:economics / Q000378:metabolism*; D005285:Fermentation; D007220:Industrial Waste / Q000191:economics / Q000517:prevention & control; D035261:Plant Components, Aerial / Q000382:microbiology*; D013045:Species Specificity; D014994:Xylose / Q000191:economics / Q000378:metabolism*; D003313:Zea mays / Q000191:economics / Q000382:microbiology*; D001617:beta-Glucosidase / Q000191:economics / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D000431:Ethanol; D014994:Xylose; D001617:beta-Glucosidase