title: Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control.
abstract: Secondary skeletal muscle fiber phenotype is dependent upon depolarization from motor neuron innervation. To study the effects of depolarization on muscle fiber type development, several in vivo and in vitro model systems exist. We have developed a relatively simple-to-use in vitro model system in which differentiated muscle cells are directly electrically stimulated at precise frequencies. This allows for single cell analysis as well as biochemical and molecular analyses of the mechanisms that control skeletal muscle phenotype.
authors: Department of Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.
authors: Eric J
authors: EJ
authors: Cavanaugh
authors: Jennifer R
authors: JR
authors: Crew
authors: Joseph X
authors: JX
authors: DiMario
mesh_terms: D002469:Cell Separation / Q000379:methods; D004558:Electric Stimulation / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000502:physiology*; D061251:Primary Cell Culture