title: Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene.
abstract: Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.
authors: National Institute of Crop Science, Rural Development Administration, Suwon 441-857, Korea.
authors: Hyang-Mi
authors: HM
authors: Park
authors: Man-Soo
authors: MS
authors: Choi
authors: Do-Yeon
authors: DY
authors: Kwak
authors: Bong-Choon
authors: BC
authors: Lee
authors: Jong-Hee
authors: JH
authors: Lee
authors: Myeong-Ki
authors: MK
authors: Kim
authors: Yeon-Gyu
authors: YG
authors: Kim
authors: Dong-Bum
authors: DB
authors: Shin
authors: Soon-Ki
authors: SK
authors: Park
authors: Yul-Ho
authors: YH
authors: Kim
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D020868:Gene Silencing; D005814:Genes, Viral; D020022:Genetic Predisposition to Disease; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000517:prevention & control* / Q000821:virology; D030821:Plants, Genetically Modified; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D029364:Tenuivirus / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D018749:RNA, Plant; D034741:RNA, Small Interfering; D017361:Viral Nonstructural Proteins