title: Development and evaluation of lipid microbubbles targeted to alpha(v)beta(3)-integrin via biotin-avidin bridge.
abstract: This work was to report preparation and quality control of targeted microbubbles (MB(t)) via biotin-avidin bridge, specifically verification of the "biotin-avidin-biotin" sandwich structure. (1) Lipid microbubbles filled with sulphur hexafluoride were produced by sonication and compared with commercially available microbubbles. (2) MB(t) were produced via biotin-avidin bridge. Specifically, the "biotin-avidin-biotin" sandwich structure on the surface of MB(t) was verified in vitro using fluorescence. (3) Adhesion of alpha(v)beta(3)-integrin targeted MB(t) (MB(αvβ3)) to human umbilical vein endothelial cells (HUVECs) was tested using the parallel plate flow chamber (PPFC). "Biotin-avidin" is a reliable method to attach molecular probe onto the surface of microbubbles.
authors: Department of Medical Ultrasonics, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors: Wei
authors: W
authors: Wang
authors: Guang-Jian
authors: GJ
authors: Liu
authors: Xiao-Yan
authors: XY
authors: Xie
authors: Zuo-Feng
authors: ZF
authors: Xu
authors: Li-Da
authors: LD
authors: Chen
authors: Guang-Liang
authors: GL
authors: Huang
authors: Lu-Yao
authors: LY
authors: Zhou
authors: Ming-De
authors: MD
authors: Lu
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D002478:Cells, Cultured; D003287:Contrast Media; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement; D039302:Integrin alphaVbeta3 / Q000008:administration & dosage* / Q000737:chemistry; D045423:Microbubbles; D009369:Neoplasms / Q000098:blood supply; D009389:Neovascularization, Pathologic / Q000175:diagnosis
chemical_list: D003287:Contrast Media; D039302:Integrin alphaVbeta3; D001360:Avidin; D001710:Biotin