title: A proteomics approach to the study of the molecular consequence of IgE-mediated cell signalling in RBL-2H3.1 cells and 2D reference map preparation for the RBL-2H3.1 cell line.
abstract: A highly reproducible 2D (two-dimensional) map for the proteome and a pattern of protein phosphorylation of high secretory variant of RBL-2H3 cells (RBL-2H3.1) (a model cell in allergy studies) in resting and treated cells with IgE or IgE+Ag are presented. Major molecular changes were seen in the proteome of 3 h-activated cells with IgE+Ag, especially for proteins of ∼17 kDa compared with the control. We have identified 13 proteins on 11 corresponding spots as up-regulated proteins in response to IgE+Ag activation. Also, protein identification on 55 spots with MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) and ESI-MS (electrospray ionization mass spectrometry) resulted in a reliable 2D reference map and an opportunity for the subsequent use of a 1 min-activated cell map for a phosphoproteomics study.
authors: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. sadroddiny@tums.ac.ir
authors: Esmaeil
authors: E
authors: Sadroddiny
authors: Arthur J G
authors: AJ
authors: Moir
authors: Birgit A
authors: BA
authors: Helm
mesh_terms: D019297:2,4-Dinitrophenol / Q000737:chemistry; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018796:Immunoconjugates / Q000276:immunology / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000276:immunology / Q000494:pharmacology*; D008954:Models, Biological; D010766:Phosphorylation / Q000187:drug effects; D020543:Proteome / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D040901:Proteomics; D012709:Serum Albumin / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018796:Immunoconjugates; D020543:Proteome; D012709:Serum Albumin; D007073:Immunoglobulin E; D019297:2,4-Dinitrophenol