title: Homology models of four Agaricus bisporus tyrosinases.
abstract: Partially purified tyrosinase from the white button mushroom Agaricus bisporus is available commercially and is a widely used experimental model for the study of tyrosinase. The structure of an H(2)L(2) tetrameric form of the mushroom enzyme was recently determined by X-ray crystallography. In this structure the two H subunits originate from the PPO3 gene, and the two L subunits are formed by a protein of unknown function with a lectin-like fold. However, the X-ray structures and oligomeric states of the mushroom PPO1, PPO2, PPO4, and PPO5 gene products remain unknown. Commercial mushroom tyrosinase powder is a mixture containing several or all of these tyrosinases, so knowledge of their structures should provide insight regarding interpretation of experimental data generated using commercial preparations of the enzyme. The PPO3 structure (H-subunit) was used as a template to generate homology models for the structures of the other four tyrosinases, and the resulting structural models were evaluated. Due to the moderate to high percentage of sequence identity (~37-76%) between PPO3 and the other four tyrosinases, the backbone conformations of the predicted structures are very similar to that of PPO3. The alpha carbons of the six copper-coordinating histidines in the active site are positioned properly in the predicted structures, but their side chains are not oriented optimally for copper binding in some cases. Thus, the models are likely to provide an accurate representation of the actual tertiary structures, but they may have limited use in studies involving docking of substrates or inhibitors in the active site. Comparison of the homology models to the structure of molluscan hemocyanin enabled a prediction of the orientation of the enzyme's C-terminal domain over the active site in the latent enzyme.
authors: Department of Chemistry and Physics, Indiana State University, Terre Haute, IN 47809, United States. jinlow@indstate.edu
authors: Jennifer K
authors: JK
authors: Inlow
mesh_terms: D000364:Agaricus / Q000201:enzymology*; D000595:Amino Acid Sequence; D002244:Carbon / Q000737:chemistry; D020134:Catalytic Domain; D004156:Catechol Oxidase / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods; D037102:Lectins / Q000737:chemistry; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D012984:Software; D014965:X-Rays
chemical_list: D037102:Lectins; D059808:Polyphenols; D002244:Carbon; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase