title: Surface-biofunctionalized multicore/shell CdTe@SiO(2) composite particles for immunofluorescence assay.
abstract: Strongly fluorescent multicore/shell structured CdTe@SiO(2) composite particles of ∼ 50 nm were synthesized via the reverse microemulsion method by using CdTe quantum dots co-stabilized by thioglycolic acid and thioglycerol. The optical stability of the CdTe@SiO(2) composite particles in a wide pH range, under prolonged UV irradiation in pure water, or in different types of physiological buffers was systematically investigated. Towards immunofluorescence assay, both poly(ethylene glycol) (PEG) and carboxyl residues were simultaneously grafted on the surface of the silanol-terminated CdTe@SiO(2) composite particles upon further reactions with silane reagents bearing a PEG segment and carboxyl group, respectively, in order to suppress the nonspecific interactions of the silica particles with proteins and meanwhile introduce reactive moieties to the fluorescent particles. Agarose gel electrophoresis, dynamic light scattering and conventional optical spectroscopy were combined to investigate the effectiveness of the surface modifications. Via the surface carboxyl residue, various antibodies were covalently conjugated to the fluorescent particles and the resultant fluorescent probes were used in detecting cancer cells through both direct fluorescent antibody and indirect fluorescent antibody assays, respectively.
authors: Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, People's Republic of China.
authors: Lihong
authors: L
authors: Jing
authors: Yilin
authors: Y
authors: Li
authors: Ke
authors: K
authors: Ding
authors: Ruirui
authors: R
authors: Qiao
authors: Andrey L
authors: AL
authors: Rogach
authors: Mingyuan
authors: M
authors: Gao
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006258:Head and Neck Neoplasms / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D000077195:Squamous Cell Carcinoma of Head and Neck; D013499:Surface Properties; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D013691:Tellurium; C028337:cadmium telluride