title: Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects.
abstract: OBJECTIVE
  To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP).
  
  
  METHODS
  Twenty-five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analysed using multiplex bead immunoassay for: GM-CSF, IFN-γ, IL-10, IL-1β, IL-2, IL-6 and TNF-α. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analysed using the Wilcoxon test and between groups using the Mann-Whitney test.
  
  
  RESULTS
  Significant reductions in GCF GM-CSF, IL-1β and the ratio IL-1β/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups.
  
  
  CONCLUSIONS
  Periodontal therapy improved GCF cytokine profiles by lowering IL-1β and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines.
authors: Department of Periodontology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors: Ana Paula
authors: AP
authors: de Lima Oliveira
authors: Marcelo
authors: M
authors: de Faveri
authors: Lauren Christine
authors: LC
authors: Gursky
authors: Maria Josefa
authors: MJ
authors: Mestnik
authors: Magda
authors: M
authors: Feres
authors: Anne D
authors: AD
authors: Haffajee
authors: Sigmund S
authors: SS
authors: Socransky
authors: Ricardo Palmier
authors: RP
authors: Teles
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D000658:Amoxicillin / Q000627:therapeutic use; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D012534:Dental Scaling; D004311:Double-Blind Method; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000378:metabolism; D016753:Interleukin-10 / Q000032:analysis / Q000378:metabolism; D053583:Interleukin-1beta / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis / Q000378:metabolism; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism; D008297:Male; D008795:Metronidazole / Q000627:therapeutic use; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D016207:Cytokines; D053583:Interleukin-1beta; D007376:Interleukin-2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D008795:Metronidazole; D000658:Amoxicillin; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor