title: Cooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblasts.
abstract: BACKGROUND
  Enhancing osteogenic capabilities of bone matrix for the treatment of fractures and segmental defects using growth factors is an active area of research. Recently, synthetic peptides like AC- 100, TP508 or p-15 corresponding to biologically active sequences of matrix proteins have been proven to stimulate bone formation. The platelet-derived growth factor (PDGF) BB has been identified as an important paracrine factor in early bone healing. We hypothesized that the combined use of PDGF-BB with synthetic peptides could result in an increase in proliferation and calcification of osteoblast-like cells.
  
  
  METHODS
  Osteoblast-like cell cultures were treated with PDGF and synthetic peptides, singly and as combinations, and compared to non-treated control cell cultures. The cultures were evaluated at days 2, 5, and 10 in terms of cell proliferation, calcification and gene expression of alkaline phosphate, collagen I and osteocalcin.
  
  
  RESULTS
  Experimental findings revealed that the addition of PDGF, p-15 and TP508 and combinations of PDGF/AC-100, PDGF/p-15 and PDGF/TP508 resulted in an increase in proliferating osteoblasts, especially in the first 5 days of cultivation. Proliferation did not significantly differ between single factors and factor combinations (p > 0.05). The onset of calcification in osteoblasts occurred earlier and was more distinct compared to the corresponding control or PDGF stimulation alone. Significant difference was found for the combined use of PDGF/p-15 and PDGF/AC-100 (p < 0.05).
  
  
  CONCLUSIONS
  Our findings indicate that PDGF exhibits cooperative effects with synthetic peptides in differentiation and proliferation. These cooperative effects cause a significant early calcification of osteoblast-like cells (p < 0.05). We suggest the combination of synthetic peptides and PDGF as a potential clinical approach for accelerating bone healing or coating osteosynthesis materials.
authors: Department of Trauma, Hand and Reconstructive Surgery, University Hospital Münster, Germany. thomas.vordemvenne@ukmuenster.de
authors: Thomas
authors: T
authors: Vordemvenne
authors: Jürgen R J
authors: JR
authors: Paletta
authors: Rene
authors: R
authors: Hartensuer
authors: Thomas
authors: T
authors: Pap
authors: Michael J
authors: MJ
authors: Raschke
authors: Sabine
authors: S
authors: Ochman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000077214:Becaplermin; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000138:chemical synthesis / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis / Q000502:physiology*; D013917:Thrombin / Q000138:chemical synthesis / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D020574:Proto-Oncogene Proteins c-sis; C407062:cell-binding peptide P-15; D000077214:Becaplermin; D003094:Collagen; C412222:rusalatide acetate; D013917:Thrombin