title: Effects of yiqi chutan tang on the proteome in Lewis lung cancer in mice.
abstract: In order to verify effects of yiqi chutan tang on lung cancer and assess molecular mechanisms involved we focused on size, tumor weight and the numbers of lung metastases and differential expression protein spot information acquired by two-way fluorescence with a tumor difference gel electrophoresis (2D-DIGE) system, and differentially expressed proteins were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-TOF). Differences were finally verified by Western blot and fluorescence quantitative PCR. We found that tumor size, tumor weight in yiqi chutan tang treatment group were significantly less than that in model group (p<0.01), with a tumor growth inhibition rate of 57.2%. For gel diagram analysis of 2D-DIGE system, compared with model group, there were 44 expressed differentially protein spots, of which 6 were up-regulated and 38 were down-regulated. Among these proteins, 37 (30 down-regulated and 7 up-regulated) were successfully identified by MALDI-TOF-TOF. In conclusion, yiqi chutan tang effects on Lewis lung cancer appeared highly related to down-regulated expression of Hspd1, prolyl 4-hydroxylase, protein disulfide-isomerase A3 precursor, EG433182, heat shock protein 5 precursor, heat shock protein 9 and stress-induced phosphoprotein 1.
authors: Oncology Department, First Clinical Medical Institute, Guangzhou University of Chinese Medicine, Guangzhou, PR China.
authors: Shumei
authors: S
authors: Wang
authors: Lizhu
authors: L
authors: Lin
authors: Jinxu
authors: J
authors: Zhou
authors: Shaoquan
authors: S
authors: Xiong
authors: Daihan
authors: D
authors: Zhou
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D004365:Drugs, Chinese Herbal; D009363:Neoplasm Proteins; D020543:Proteome