title: Direct analysis of underivatized amino acids in plant extracts by LC-MS-MS.
abstract: In this chapter, we describe a method for quantification of 20 proteinogenic amino acids as well as 13 (15)N-labeled amino acids by liquid chromatography-mass spectrometry without the need for derivatization and use of organic solvents. Analysis of the underivatized amino acids is performed by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) in the positive ESI mode. Separation is achieved on a strong cation exchange (SCX) column (Luna 5 μm SCX 100 Å) with 30 mM ammonium acetate in water (A) and 5% acetic acid in water (B). Quantification is accomplished by use of d(5)-phenylalanine as internal standard achieving limits of detection of 0.1-3.0 μM. The method was successfully applied for the determination of proteinogenic and (15)N-labeled amino acids in plant extracts.
authors: Institute of Bio- and Geosciences, Forschungszentrum Jülich GmbH, ICG-3, Jülich, Germany. b.thiele@fz-juelich.de
authors: Björn
authors: B
authors: Thiele
authors: Nadine
authors: N
authors: Stein
authors: Marco
authors: M
authors: Oldiges
authors: Diana
authors: D
authors: Hofmann
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D010936:Plant Extracts / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards
chemical_list: D000596:Amino Acids; D010936:Plant Extracts; D012996:Solutions