title: Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice.
abstract: Gene inactivation has become the gold standard for determining gene function in the mouse. Many genes inactivated in the germ line cause early lethality that precludes phenotypic assessment at a later time point. Conditional gene inactivation using Cre recombinase expressed via a tissue specific promoter/enhancer allows phenotypic analyses of selected tissues, but lacks temporal control. Recent development of the tamoxifen-inducible Cre-ER (T2) offers both cell type-specific and temporal control of conditional gene inactivation. As an example, we describe the design and step-wise construction of a Cre-ER (T2) knock-in allele at the Pax7 locus using the recombineering method - Pax7 is selectively expressed in embryonic muscle progenitors and adult muscle stem cells. The resulting Pax7-Cre- ER (T2) (Pax7 (CE)) allele has been successfully applied to embryos and adults for tamoxifen-regulated myogenic lineage tracing and gene inactivation (Nature 460:627-631, 2009; Genesis 48:424-436, 2010).
authors: Department of Embryology, Carnegie Institution of Washington, Baltimore, MD, USA.
authors: Christoph
authors: C
authors: Lepper
authors: Chen-Ming
authors: CM
authors: Fan
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D059765:Homologous Recombination; D019426:Integrases / Q000235:genetics*; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D051763:PAX7 Transcription Factor / Q000235:genetics; D017422:Sequence Analysis, DNA; D013629:Tamoxifen / Q000494:pharmacology*; D014169:Transformation, Bacterial
chemical_list: D051763:PAX7 Transcription Factor; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases