title: Comparative proteomic analysis revealed metabolic changes and the translational regulation of Cry protein synthesis in Bacillus thuringiensis.
abstract: B. thuringiensis strain YBT-1520 is highly toxic to some Lepidopteran pests and is used to control pests in southern part of China. The aim of this work is to uncover the metabolic changes associated with the ICP synthesis. A comparative proteomic analysis on the strain was performed with 2-DE and MALDI-TOF-MS methods with the bacterial culture grown in a modified defined minimal medium. Transcriptional expression of some key enzymes for carbohydrate metabolism was also investigated with qPCR. Seventy-two proteins differentially expressed at least two folds were identified. Significant changes were observed in metabolisms when comparing stationary growth phase to mid-log phase. In late-stationary phase, five major phenomena were observed: 1) the glycolysis cycle was redirected into pentose phosphate pathway and ended in serine formation; 2) the tricarboxylic acid (TCA) cycle was suppressed; 3) poly(3-hydroxybutyrate) (PHB) was considered to be the important energy and carbon supply; 4) collectively increased proteases suggested massive protein degradation to release amino acids, and 5) translation-related factors were up-regulated including EF-Tu, GroEL and GatB, while the negative regulator YfiA was down. The metabolic changes of bacterium during growth shift indicated that the cells acquired energy, amino acid precursors and translation-related factors for the ICP synthesis.
authors: State Key Laboratory of Agricultural Microbiology and National Engineering Research Center for Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province, PR China.
authors: Yuhua
authors: Y
authors: Gong
authors: Mingshun
authors: M
authors: Li
authors: Dong
authors: D
authors: Xu
authors: Hui
authors: H
authors: Wang
authors: Jin
authors: J
authors: He
authors: Dandan
authors: D
authors: Wu
authors: Deju
authors: D
authors: Chen
authors: Ning
authors: N
authors: Qiu
authors: Qiyu
authors: Q
authors: Bao
authors: Ming
authors: M
authors: Sun
authors: Ziniu
authors: Z
authors: Yu
mesh_terms: D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins; D002241:Carbohydrates / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006019:Glycolysis; D019476:Insect Proteins / Q000378:metabolism; D008954:Models, Biological; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013696:Temperature; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; C095416:Cry toxin receptors; D019476:Insect Proteins; D011956:Receptors, Cell Surface