title: The SDF-1 3'a genetic variation of the chemokine SDF-1α (CXCL12) in parallel with its increased circulating levels is associated with susceptibility to MS: a study on Iranian multiple sclerosis patients.
abstract: Immune system-related factors are important in pathogenesis of multiple sclerosis. The CXC chemokine SDF-1α (CXCL12) is involved in the immune responses. Hence, the aim of this study was to investigate the association between serum levels of SDF-1α (CXCL12) and its gene polymorphisms at position +801 with multiple sclerosis. In this experimental study, blood samples were collected from 100 multiple sclerosis patients and 100 healthy controls on EDTA pre-coated tubes. DNA was extracted and DNA samples were analyzed for SDF-1α (CXCL12) polymorphisms using PCR-RLFP in patients and controls. The serum levels of SDF-1α (CXCL12) were measured by ELISA. Demographic data were also collected by a questionnaire which was designed specifically for this study. Our results showed a significant difference between the A/A, A/G, and G/G genotype and A and G alleles of polymorphisms at position +801 of SDF-1α (CXCL12). Our results also showed that serum levels of SDF-1α (CXCL12) were markedly higher in patients than healthy controls, but no association was observed between SDF-1α (CXCL12) polymorphism and its serum levels. The results of this study might suggest the serum levels of SDF-1α (CXCL12) and its polymorphism play an important role in pathogenesis of multiple sclerosis. It is also worth noting that these factors could probably use as pivotal biological markers in the diagnosis of MS.
authors: Department of Neurology, Ali-ebn-Abitaleb Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors: Hossein
authors: H
authors: Azin
authors: Reza
authors: R
authors: Vazirinejad
authors: Behzad Nasiri
authors: BN
authors: Ahmadabadi
authors: Hossein
authors: H
authors: Khorramdelazad
authors: Ebrahim Rezazadeh
authors: ER
authors: Zarandi
authors: Mohammad Kazemi
authors: MK
authors: Arababadi
authors: Mojgan Noroozi
authors: MN
authors: Karimabad
authors: Ali
authors: A
authors: Shamsizadeh
authors: Houshang
authors: H
authors: Rafatpanah
authors: Gholamhossein
authors: G
authors: Hassanshahi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D054377:Chemokine CXCL12 / Q000097:blood* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12