title: Morphological differences in human zygotes and embryos cultured in different media.
abstract: OBJECTIVE
  To compare the effects of four culture media on the quality of human zygotes and embryos.
  
  
  METHODS
  Prospective study analyzing 2289 human embryos cultivated simultaneously in two different culture media: HTF, the default medium, with either Universal IVF, Global or IVF-30 as the secondary media. The sibling oocytes by each patient were randomly divided between the two culture media following intracytoplasmic sperm injection (ICSI). On day 1 the pronuclear stage of zygotes were evaluated and on day 2 embryos were evaluated according to the number of cells, percentage of fragmentation and number of nuclei. Z-test and odds ratios were used in the statistical analysis.
  
  
  RESULTS
  There was a higher percentage (55.2%) of class A1 + A2 zygotes with IVF-30 compared with HTF, Global or Universal IVF media (49.1%, 44.7% and 44.2%, respectively). The percentage of Top embryos was significantly higher with Global (40.2%) compared with HTF (21.3%), IVF-30 (25.0%) or Universal IVF media (11.2%).
  
  
  CONCLUSIONS
  Global medium produced more Top embryos evaluated on day 2 of development.
authors: Campinas Center of Human Reproduction, Campinas, São Paulo, Brazil.
authors: Raquel Di Falco
authors: RD
authors: Cossiello
authors: Alexandros
authors: A
authors: Aggelis
authors: Daniel
authors: D
authors: Faúndes
authors: Carlos A
authors: CA
authors: Petta
mesh_terms: D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000254:growth & development; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D015053:Zygote / Q000166:cytology* / Q000378:metabolism
chemical_list: D003470:Culture Media