title: Whole-genome RT-qPCR microRNA expression profiling.
abstract: MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer. Owing to their small size (20-22 nucleotides), accurate quantification of miRNA expression is particularly challenging. In this chapter, we present different RT-qPCR technologies that enable whole genome miRNA expression quantification.
authors: Center for Medical Genetics, Ghent University, De Pintelaan 185, Ghent 9000, Belgium.
authors: Pieter
authors: P
authors: Mestdagh
authors: Stefaan
authors: S
authors: Derveaux
authors: Jo
authors: J
authors: Vandesompele
mesh_terms: D000465:Algorithms; D003627:Data Interpretation, Statistical; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D015894:Genome, Human; D006801:Humans; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000592:standards; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards
chemical_list: D035683:MicroRNAs