title: Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid.
abstract: OBJECTIVE
  We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens.
  
  
  METHODS
  Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunoglobulins A, G and M. GCF was collected for analysis of MMP-8 levels and for the PCR-analysis of the microorganisms Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Tannerella forsythia.
  
  
  RESULTS
  Periodontitis patients were more often infected by P. gingivalis (p < 0.05), P. intermedia and T. denticola (p = 0.01) than controls. Salivary albumin and protein concentrations were significantly higher in subjects with T. denticola (p < 0.05). MMP-8 levels were significantly higher in subjects with T. denticola (p < 0.001) and T. forsythia (p < 0.01). No corresponding results were found in salivary immunoglobulin concentrations.
  
  
  CONCLUSIONS
  The presence of T. denticola seemed to increase salivary albumin and total protein concentrations, and GCF levels of MMP-8. Both T. denticola and T. forsythia seemed to induce a cascade of host response with increased MMP-8 in GCF.
authors: Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, Huddinge, Sweden.
authors: Maha
authors: M
authors: Yakob
authors: Kirsti
authors: K
authors: Kari
authors: Taina
authors: T
authors: Tervahartiala
authors: Timo
authors: T
authors: Sorsa
authors: Per-Östen
authors: PÖ
authors: Söder
authors: Jukka H
authors: JH
authors: Meurman
authors: Birgitta
authors: B
authors: Söder
mesh_terms: D000418:Albumins / Q000032:analysis; D001439:Bacteroides / Q000502:physiology; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015331:Cohort Studies; D004789:Enzyme Activation; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000201:enzymology* / Q000382:microbiology*; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000378:metabolism*; D008875:Middle Aged; D010518:Periodontitis / Q000201:enzymology* / Q000382:microbiology*; D012463:Saliva / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000378:metabolism*; D012636:Secretory Rate; D048373:Treponema denticola / Q000502:physiology*
chemical_list: D000418:Albumins; D012471:Salivary Proteins and Peptides; D020784:Matrix Metalloproteinase 8