title: Analysis of signalling pathways by western blotting and immunoprecipitation.
abstract: This chapter describes the analysis of signalling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step by step guide to cell culture techniques, protein isolation, purification, measurement, electrophoretic transfer, and detection.
authors: Bone and Cancer Group, Edinburgh Cancer Research UK Centre and Rheumatic Disease Unit, the Centre of Molecular Medicine, University of Edinburgh, Edinburgh, UK. aymen.idris@ed.ac.uk
authors: Aymen I
authors: AI
authors: Idris
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D001842:Bone and Bones / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D010766:Phosphorylation; D017368:Protein Prenylation; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D011506:Proteins