title: Preferential cell response to anisotropic electro-spun fibrous scaffolds under tension-free conditions.
abstract: Anisotropic alignment of collagen fibres in musculoskeletal tissues is responsible for the resistance to mechanical loading, whilst in cornea is responsible for transparency. Herein, we evaluated the response of tenocytes, osteoblasts and corneal fibroblasts to the topographies created through electro-spinning and solvent casting. We also evaluated the influence of topography on mechanical properties. At day 14, human osteoblasts seeded on aligned orientated electro-spun mats exhibited the lowest metabolic activity (P < 0.001). At day 5 and at day 7, no significant difference was observed in metabolic activity of human corneal fibroblasts and bovine tenocytes respectively seeded on different scaffold conformations (P > 0.05). Osteoblasts and corneal fibroblasts aligned parallel to the direction of the aligned orientated electro-spun mats, whilst tenocytes aligned perpendicular to the aligned orientated electro-spun mats. Mechanical evaluation demonstrated that aligned orientated electro-spun fibres exhibited significant higher stress at break values than their random aligned counterparts (P < 0.006) and random orientated electro-spun fibres exhibited significant higher strain at break values than the aligned orientated scaffolds (P < 0.006). While maintaining fibre structure, we also developed a co-deposition method of spraying and electro-spinning, which enables the incorporation of microspheres within the three-dimensional structure of the scaffold.
authors: Network of Excellence for Functional Biomaterials (NFB), National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: A
authors: A
authors: English
authors: A
authors: A
authors: Azeem
authors: D A
authors: DA
authors: Gaspar
authors: K
authors: K
authors: Keane
authors: P
authors: P
authors: Kumar
authors: M
authors: M
authors: Keeney
authors: N
authors: N
authors: Rooney
authors: A
authors: A
authors: Pandit
authors: D I
authors: DI
authors: Zeugolis
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen; D005456:Fluorescent Dyes; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D013500:Surface Tension; D054457:Tissue Scaffolds
chemical_list: D005456:Fluorescent Dyes; D003094:Collagen