title: Hericium erinaceus suppresses LPS-induced pro-inflammation gene activation in RAW264.7 macrophages.
abstract: The aim of this study was to investigate the anti-inflammatory properties of each fraction of Hericium erinaceus (HE). The ethanol extract from HE was partitioned with different solvents in the order of increasing polarity. The treatment with 10-100 μg/mL of each fraction did not reduce RAW 264.7 cell viability except ethyl acetate fraction. Among the various extracts, the chloroform fraction showed the most potent activity against nitric oxide (NO), prostaglandin E(2) (PGE(2)) and reactive oxygen species (ROS). The western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that chloroform fraction from HE (CHE) significantly reduced the protein level of iNOS and cyclooxygenase-2 (COX-2) or mRNA levels of iNOS in lipopolysaccharide-induced macrophages. Furthermore, CHE inhibited the translocation of nuclear factor (NF)-κB p65 subunit, phsophorylation of I-κB, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. Furthermore, the activation of both activator protein-1 (AP-1) and NF κB in the nucleus were abrogated by CHE with luciferase assay. In conclusion, these results indicate that CHE may provide an anti-inflammatory effect by attenuating the generation of excessive NO, PGE(2), and ROS and by suppressing the expression of pro-inflammatory genes through the inhibition of NF-κB and JNK activity.
authors: Medicinal Crops Division, Ginseng and Medicinal Plants Research Institute Rural Development Administration, Eumseong, Republic of Korea.
authors: Young-Ock
authors: YO
authors: Kim
authors: Sang-Won
authors: SW
authors: Lee
authors: Chung-Hun
authors: CH
authors: Oh
authors: Yun-Hee
authors: YH
authors: Rhee
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D045424:Complex Mixtures; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D015232:Dinoprostone