title: Genotypic characterization of Staphylococcus aureus isolated from bovines, humans, and food in Indonesia.
abstract: The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated the prevalence of staphylococcal enterotoxin (SE) genes and other virulence gene determinants by PCR. The identification of S. aureus was based on culturing and biochemical tests, and by amplifying a specific section of the 23S rRNA gene. PCR amplification of the SE genes (sea, seb, sec, see, seg, seh, and sei) singly or in combination was observed. Most isolates of bovine origin harbored hla (84%) and cap5 (74%), while most isolates from humans harbored hla (73%), cap8 (91%), and fnbA (100%). Strains from food sources were positive for hla (100%), cap5 (100%), and cap8 (64%) unlike isolates from humans or bovines. A single enzyme AFLP analysis revealed a correlation between AFLP clusters of some strains and the source of the isolates The genotypic results of the present study might help to better understand the distribution of prevalent S. aureus clones among humans, bovines, and food and will help control S. aureus infections in Indonesia.
authors: Department of Clinical Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia. isrinasalasia@yahoo.com
authors: Siti Isrina Oktavia
authors: SI
authors: Salasia
authors: Syarifudin
authors: S
authors: Tato
authors: Ngalijan
authors: N
authors: Sugiyono
authors: Dwi
authors: D
authors: Ariyanti
authors: Feny
authors: F
authors: Prabawati
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D005260:Female; D005516:Food Microbiology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins