title: [Irbesartan regulates inflammatory gene expressions related to atherosclerosis in EA.hy926 cells].
abstract: OBJECTIVE
  To characterize if irbesartan regulates vascular inflammatory gene expression profiles related to atherosclerosis in EA.hy926 cells.
  
  
  METHODS
  Human umbilical vein endothelial cell line EA.hy926 cultured in vitro was incubated with irbesartan (1×10(-6) mol/L) for 24 h. The total RNA was extracted from the cells for gene expression profiling. The DAVID Gene Functional Classification Tool was used to analyze the disease- and function-related genes in the cells. Real-time quantitative polymerase chain reaction (RT-PCR) was used to verify the genes showing differential expression after irbesartan treatment. The protein levels of angiotensin II type 1 receptor (AT1R) and type 2 receptor (AT2R) were tested by Western blotting.
  
  
  RESULTS
  Compared with the control cells, 56 genes were found to show marked changes following irbesartan treatment, including 39 up-regulated and 17 down-regulated genes. Disease analysis suggested that these genes were related to such diseases as coronary atherosclerosis, myocardial infarction, and colorectal cancer. Eight genes, namely MMP2, PTGS2, PECAM1, SELP, SELL, CYP1A1, MMRN1, and HSPA1A, were involved in atherosclerosis and myocardial infarction. Verification by RT-PCR produced a result consistent with the gene array result. AT1R was down-regulated while AT2R up-regulated in irbesartan-treated cells.
  
  
  CONCLUSIONS
  Irbesartan regulates the inflammatory gene expressions related to atherosclerosis in EA.hy926 cells. These inflammatory factors may promote destabilization of atherosclerotic plaque possibly in relation to AT2R overexpression.
authors: Geriatric Department of Cardiology, Institute of Gerontology and Geriatrics, General Hospital of PLA, Beijing 100853, China. mwhycy@163.com
authors: Cong
authors: C
authors: Ma
authors: Xue-chun
authors: XC
authors: Lu
authors: Li
authors: L
authors: Fan
authors: Yun
authors: Y
authors: Luo
authors: Bo
authors: B
authors: Yang
authors: Yan
authors: Y
authors: Gao
authors: Xian-feng
authors: XF
authors: Liu
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D050197:Atherosclerosis / Q000235:genetics* / Q000473:pathology / Q000517:prevention & control; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D019610:Cytoprotection; D020869:Gene Expression Profiling; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D000077405:Irbesartan; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics; D013777:Tetrazoles / Q000494:pharmacology*
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D001713:Biphenyl Compounds; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D013777:Tetrazoles; D000077405:Irbesartan