title: Overexpression of the human ubiquitin E3 ligase CUL4A alleviates hypoxia-reoxygenation injury in pheochromocytoma (PC12) cells.
abstract: The ubiquitin E3 ligase CUL4A plays important roles in diverse cellular processes including carcinogenesis and proliferation. It has been reported that the expression of CUL4A can be induced by hypoxic-ischemic injury. However, the effect of elevated expression of CUL4A on hypoxia-reoxygenation injury is currently unclear. In this study, human CUL4A (hCUL4A) was expressed in rat pheochromocytoma (PC12) cells using adenoviral vector-mediated gene transfer, and the effects of hCUL4A expression on hypoxia-reoxygenation injury were investigated. In PC12 cells subjected to hypoxia and reoxygenation, we found that hCUL4A suppresses apoptosis and DNA damage by regulating apoptosis-related proteins and cell cycle regulators (Bcl-2, caspase-3, p53 and p27); consequently, hCUL4A promotes cell survival. Taken together, our results reveal the beneficial effects of hCUL4A in PC12 cells upon hypoxia-reoxygenation injury.
authors: Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, 172 Tong Zipo Road, Changsha 410013, China.
authors: Can
authors: C
authors: Tan
authors: Li-Yang
authors: LY
authors: Zhang
authors: Hong
authors: H
authors: Chen
authors: Ling
authors: L
authors: Xiao
authors: Xian-Peng
authors: XP
authors: Liu
authors: Jian-Xiang
authors: JX
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D015687:Cell Hypoxia; D044842:Cullin Proteins / Q000096:biosynthesis* / Q000235:genetics; D004249:DNA Damage; D006801:Humans; D000860:Hypoxia / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; C114234:CUL4A protein, human; D044842:Cullin Proteins; D044767:Ubiquitin-Protein Ligases; D010100:Oxygen