title: Adjuvant effect of liposome-encapsulated natural phosphodiester CpG-DNA.
abstract: Immunostimulatory CpG-DNA targeting TLR9 is one of the most extensively evaluated vaccine adjuvants. Previously, we found that a particular form of natural phosphodiester bond CpG-DNA (PO-ODN) encapsulated in a phosphatidyl-Β-oleoyl- γ-palmitoyl ethanolamine (DOPE) : cholesterol hemisuccinate (CHEMS) (1 : 1 ratio) complex (Lipoplex(O)) is a potent adjuvant. Complexes containing peptide and Lipoplex(O) are extremely useful for B cell epitope screening and antibody production without carriers. Here, we showed that IL-12 production was increased in bone marrow derived dendritic cells in a CpG sequence-dependent manner when PO-ODN was encapsulated in Lipoplex(O), DOTAP or lipofectamine. However, the effects of Lipoplex(O) surpassed those of PO-ODN encapsulated in DOTAP or lipofectamine and also other various forms of liposome-encapsulated CpG-DNA in terms of potency for protein antigen-specific IgG production and Th1- associated IgG2a production. Therefore, Lipoplex(O) may have a unique potent immunoadjuvant activity which can be useful for various applications involving protein antigens as well as peptides.
authors: Departments of Microbiology, College of Medicine, Hallym University, Chuncheon, Korea.
authors: Dongbum
authors: D
authors: Kim
authors: Sanghoon
authors: S
authors: Kwon
authors: Chi-Seok
authors: CS
authors: Ahn
authors: Younghee
authors: Y
authors: Lee
authors: Soo-Young
authors: SY
authors: Choi
authors: Jinseu
authors: J
authors: Park
authors: Hyeok-Yil
authors: HY
authors: Kwon
authors: Hyung-Joo
authors: HJ
authors: Kwon
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002412:Cations; D004247:DNA / Q000276:immunology*; D003713:Dendritic Cells / Q000187:drug effects; D015226:Dinucleoside Phosphates / Q000276:immunology*; D004952:Esters / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis; D008081:Liposomes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D002412:Cations; D004247:DNA; D015226:Dinucleoside Phosphates; D004952:Esters; D007074:Immunoglobulin G; D018664:Interleukin-12; D008081:Liposomes; D009113:Muramidase; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin; C408982:CPG-oligonucleotide; C015772:CpG dinucleotide; C421864:hen egg lysozyme