title: Raman spectroscopic characterization and differentiation of seminal plasma.
abstract: Raman spectroscopy (RS) was applied for the analysis of seminal plasma in order to detect spectral parameters, which might be used for differentiating the normal and abnormal semen samples. Raman spectra of seminal plasma separated from normal and abnormal semen samples, showed a distinct difference in peak ratios between 1449 and 1418 cm(-1) (P < 0.05). More efficient alternative method of using principal component analysis-linear discriminate analysis based on Raman spectroscopic data yielded a diagnostic sensitivity of 73% and specificity of 82%. The results suggest that RS combined with the multivariate analysis method has the potential for differentiating semen samples by examination of the corresponding seminal plasma.
authors: Zufang
authors: Z
authors: Huang
authors: Xiwen
authors: X
authors: Chen
authors: Yanping
authors: Y
authors: Chen
authors: Jinhua
authors: J
authors: Chen
authors: Min
authors: M
authors: Dou
authors: Shangyuan
authors: S
authors: Feng
authors: Haishan
authors: H
authors: Zeng
authors: Rong
authors: R
authors: Chen
mesh_terms: D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D012372:ROC Curve; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*