title: Clinical significance of microRNA-155 expression in human breast cancer.
abstract: BACKGROUND
  The aim of this study is to investigate clinical significance of miR-155 expression in breast cancer.
  
  
  METHODS
  TaqMan real-time RT-PCR was performed to detect miR-155 expression in breast cancer tissues. The correlation of miR-155 expression with clinicopathological factors and prognosis of breast cancer patients was analyzed. Then, the prognostic value of miR-155 expression was analyzed by univariate and multivariate analyses. Moreover, the effect of miR-155 expression on phenotypes of breast cancer cell was determined by antisense technology.
  
  
  RESULTS
  The relative expression of miR-155 was significantly higher in breast cancer tissues than in corresponding nontumor tissues. High miR-155 expression was correlated with higher tumor grade, advanced tumor stage and lymph node metastasis (P = 0.012, 0.001, and 0.003, respectively). Kaplan-Meier survival analysis indicated that the disease-free and overall survival rates of high miR-155 group were significantly lower than those of low miR-155 group (P = 0.038 and 0.029, respectively). Multivariate analysis showed that high miR-155 expression was a poor prognostic factor (P = 0.009). Furthermore, antisense targeting miR-155 could inhibit growth, induce cell arrest in G(0) /G(1) phase, enhance apoptosis, and increase radiosensitivity in breast cancer cells.
  
  
  CONCLUSIONS
  MiR-155 expression might be an independent prognostic factor and a therapeutic target for human breast cancer.
authors: Department of General Surgery, The First Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, China.
authors: Jian
authors: J
authors: Chen
authors: Bing-Chan
authors: BC
authors: Wang
authors: Jin-Hai
authors: JH
authors: Tang
mesh_terms: D017209:Apoptosis; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs