title: Proteomic analysis of colon tissue from interleukin-10 gene-deficient mice fed polyunsaturated Fatty acids with comparison to transcriptomic analysis.
abstract: Inflammatory bowel disease (IBD) is characterized by intestinal inflammation and is believed to involve complex interactions between genetic, immunological, and environmental factors. We measured changes in the proteome associated with bacterially induced intestinal inflammation in the interleukin 10 gene-deficient (Il10(-/-)) mouse model of IBD, established effects of the dietary polyunsaturated fatty acids (PUFAs) n-3 eicosapentaenoic acid (EPA) and n-6 arachidonic acid (AA) on protein expression (using oleic acid as a control fatty acid), and compared these changes with previously observed transcriptome changes in the same model. Ingenuity pathways analysis of proteomics data showed bacterially induced inflammation was associated with reduced expression of proteins from pathways of metabolism and digestion/absorption/excretion of nutrients/ions, and increased expression of cellular stress and immune response proteins. Both PUFA treatments showed anti-inflammatory activity; EPA appeared to act via the PPARα pathway, whereas AA appeared to increase energy metabolism and cytoskeletal organization and reduce cellular stress responses, possibly enabling a more robust response to inflammation. While there was agreement between proteomic and transcriptomic data with respect to pathways, there was limited concordance between individual gene and protein data, reflecting the importance of having both gene and protein data to better understand complex diseases such as IBD.
authors: Biological Chemistry & Bioactives, Food Innovation, The New Zealand Institute for Plant & Food Research Ltd , Ruakura Private Bag 3123, Waikato Mail Centre, Hamilton 3240, New Zealand.
authors: Janine M
authors: JM
authors: Cooney
authors: Matthew P G
authors: MP
authors: Barnett
authors: Diane
authors: D
authors: Brewster
authors: Bianca
authors: B
authors: Knoch
authors: Warren C
authors: WC
authors: McNabb
authors: William A
authors: WA
authors: Laing
authors: Nicole C
authors: NC
authors: Roy
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D016000:Cluster Analysis; D003106:Colon / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000378:metabolism / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D020869:Gene Expression Profiling; D007249:Inflammation; D016753:Interleukin-10 / Q000172:deficiency* / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019301:Oleic Acid / Q000378:metabolism; D011506:Proteins; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D004042:Dietary Fats, Unsaturated; D011506:Proteins; D020543:Proteome; D016753:Interleukin-10; D016718:Arachidonic Acid; D019301:Oleic Acid; D015118:Eicosapentaenoic Acid