title: Effect of DETA-NONOate and papaverine on vasodilation of human internal mammary artery.
abstract: In this study, the relaxatory effect of DETA-NONOate is compared with that of papaverine on isolated human internal mammary artery. We investigated the inhibitory effects of DETA-NONOate and papaverine on phenylephrine-induced contractile response in internal mammary artery segments. The internal mammary artery segments, taken from methodologically matched patients who underwent coronary artery bypass grafting, were prepared, placed in an organ bath, and contracted with phenylephrine (10(-9) to 10(-4) mol/L) to investigate their relaxatory response to DETA-NONOate or papaverine. Phenylephrine dose-response contraction was obtained after 1, 2, and 3 h in segments pre-incubated with DETA-NONOate or papaverine for 30 min. The EC50 that presented for internal mammary artery segments incubated with DETA-NONOate was 3.523 ± 1.2696 × 10(-7) mol/L, and for papaverine was 3.467 ± 1.2145 × 10(-6) mol/L. In segments pre-incubated with DETA-NONOate, the contractile response to phenylephrine was suppressed in the first 2 h post-incubation, compared with control responsive groups (p < 0.05), but this inhibition was revoked after 3 h post-incubation. We showed that DETA-NONOate has a more significant relaxative effect by comparison with papaverine; moreover, continuous and long-lasting nitric oxide production by DETA-NONOate might be of great importance for the outcome from coronary artery bypass grafting, when internal mammary artery is used as a conduit.
authors: a Tehran Heart Center, Tehran University of Medical Sciences, Tehran 1411713138, Iran.
authors: Nastaran
authors: N
authors: Rahimi
authors: Ahmad R
authors: AR
authors: Dehpour
authors: Mehrak
authors: M
authors: Javadi-Paydar
authors: Hamid
authors: H
authors: Sohanaki
authors: Shahram
authors: S
authors: Rabbani
authors: Mohammad
authors: M
authors: Ansari
authors: Seyed Hossein Ahmadi
authors: SH
authors: Tafti
mesh_terms: D000368:Aged; D002316:Cardiotonic Agents / Q000009:adverse effects; D006801:Humans; D008297:Male; D008323:Mammary Arteries / Q000187:drug effects* / Q000502:physiology; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D009603:Nitroso Compounds / Q000494:pharmacology*; D010208:Papaverine / Q000494:pharmacology*; D010656:Phenylephrine / Q000009:adverse effects; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D009603:Nitroso Compounds; D014665:Vasodilator Agents; C094210:2,2'-(hydroxynitrosohydrazono)bis-ethanamine; D010656:Phenylephrine; D009569:Nitric Oxide; D010208:Papaverine