title: In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence.
abstract: Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.
authors: University of Lübeck, Institute of Biomedical Optics, Lübeck, Germany.
authors: Regina
authors: R
authors: Orzekowsky-Schroeder
authors: Antje
authors: A
authors: Klinger
authors: Björn
authors: B
authors: Martensen
authors: Maike
authors: M
authors: Blessenohl
authors: Andreas
authors: A
authors: Gebert
authors: Alfred
authors: A
authors: Vogel
authors: Gereon
authors: G
authors: Hüttmann
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D005260:Female; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000737:chemistry / Q000166:cytology*; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D010581:Peyer's Patches / Q000737:chemistry / Q000166:cytology