title: Estimating the density of fluorescent nanoparticles in the primo vessels in the fourth ventricle and the spinal cord of a rat.
abstract: The primo vascular system is a novel circulatory system forming a network throughout an animal's body. Primo vessels were recently observed in the fourth ventricle of the brain and in the spinal cord of a rat by using fluorescent nanoparticles. In order to quantify the nanoparticles in the primo vessels, we measured the florescence of the nanoparticles and calibrated the measurements by using a reference suspension. We removed the noise due to autofluorescence with the technique of multispectral imaging. The line densities of nanoparticles and the contrast values of their images were, respectively, 0.5 ± 0.5 ng/mm and 0.7 ± 0.5 for primo vessels in the fourth ventricle, and 1.3 ± 0.6 ng/mm and 1.4 ± 0.2 for primo vessels in the spinal cord. The data obtained from and the procedures used in this work could be useful in evaluating the feasibility of using nanoparticles as a contrast agent during MRI or CT imaging of primo vessels in the brain or the spinal cord.
authors: Seoul National University, Nano Primo Research Center, Advanced Institutes of Convergence Technology, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea.
authors: Jaekwan
authors: J
authors: Lim
authors: Jong Hyun
authors: JH
authors: Jung
authors: Sungwoo
authors: S
authors: Lee
authors: Zhendong
authors: Z
authors: Su
authors: Zhou
authors: Z
authors: Qiang
authors: Jin-Myung
authors: JM
authors: Cha
authors: Jin-Kyu
authors: JK
authors: Lee
authors: Kwang-Sup
authors: KS
authors: Soh
mesh_terms: D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D020546:Fourth Ventricle / Q000033:anatomy & histology* / Q000737:chemistry; D008297:Male; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes