title: Identification of DLG5 and SLC22A5 gene polymorphisms in Malaysian patients with Crohn's disease.
abstract: OBJECTIVE
  The aim of this study was to investigate the association of DLG5 and SLC22A5 gene polymorphisms with the onset of Crohn's disease (CD) in a Malaysian cohort.
  
  
  METHODS
  Genomic DNA of 80 CD patients and 100 healthy unrelated control individuals was extracted and analyzed via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on DLG5 (4136 C/A), DLG5_e26 and SLC22A5 (-207 G/C) genetic polymorphisms. Data obtained from the study were then subjected to statistical analysis to test for risk association.
  
  
  RESULTS
  Significant associations of both DLG5 polymorphisms with the development of CD in the Malaysian patients were observed in this study. The homozygous C genotype of the DLG5 polymorphism was significantly related to CD patients (P = 0.0023, OR = 2.5320), while the homozygous A was significant in control individuals (P = 0.0224, OR = 0.4480). In DLG5_e26 polymorphisms, we found a significant distribution of the homozygous insA genotype in CD patients (P = 0.0006, OR = 2.8916), whereas the heterozygous insA/delA genotype was significant in controls (P = 0.0007, OR = 0.3487). We hypothesized that there might be a complex interaction of both alleles, which confered a protective effect against the onset of CD. However, we did not observe any significant correlation of SLC22A5 polymorphisms with this disease.
  
  
  CONCLUSIONS
  In our study, both polymorphisms in the DLG5 gene were found to be associated with CD patients in Malaysia. Therefore, these loci can be potentially used as susceptibility markers in the Malaysian population.
authors: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. khchua@um.edu.my
authors: Kek Heng
authors: KH
authors: Chua
authors: Lay Hoong
authors: LH
authors: Lian
authors: Boon Pin
authors: BP
authors: Kee
authors: Chooi Mei
authors: CM
authors: Thum
authors: Way Seah
authors: WS
authors: Lee
authors: Ida
authors: I
authors: Hilmi
authors: Khean Lee
authors: KL
authors: Goh
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D015331:Cohort Studies; D003424:Crohn Disease / Q000208:ethnology* / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics; D005838:Genotype; D006801:Humans; D008296:Malaysia; D008565:Membrane Proteins / Q000235:genetics*; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D000074058:Solute Carrier Family 22 Member 5; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C114461:DLG5 protein, human; D008565:Membrane Proteins; D027701:Organic Cation Transport Proteins; C492020:SLC22A5 protein, human; D000074058:Solute Carrier Family 22 Member 5; D025521:Tumor Suppressor Proteins