title: Scale-up and integration of alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis, and ethanolic fermentation.
abstract: Alkaline hydrogen peroxide (AHP) has several attractive features as a pretreatment in the lignocellulosic biomass-to-ethanol pipeline. Here, the feasibility of scaling-up the AHP process and integrating it with enzymatic hydrolysis and fermentation was studied. Corn stover (1 kg) was subjected to AHP pretreatment, hydrolyzed enzymatically, and the resulting sugars fermented to ethanol. The AHP pretreatment was performed at 0.125 g H(2) O(2) /g biomass, 22°C, and atmospheric pressure for 48 h with periodic pH readjustment. The enzymatic hydrolysis was performed in the same reactor following pH neutralization of the biomass slurry and without washing. After 48 h, glucose and xylose yields were 75% and 71% of the theoretical maximum. Sterility was maintained during pretreatment and enzymatic hydrolysis without the use of antibiotics. During fermentation using a glucose- and xylose-utilizing strain of Saccharomyces cerevisiae, all of the Glc and 67% of the Xyl were consumed in 120 h. The final ethanol titer was 13.7 g/L. Treatment of the enzymatic hydrolysate with activated carbon prior to fermentation had little effect on Glc fermentation but markedly improved utilization of Xyl, presumably due to the removal of soluble aromatic inhibitors. The results indicate that AHP is readily scalable and can be integrated with enzyme hydrolysis and fermentation. Compared to other leading pretreatments for lignocellulosic biomass, AHP has potential advantages with regard to capital costs, process simplicity, feedstock handling, and compatibility with enzymatic deconstruction and fermentation. Biotechnol. Bioeng. 2012; 109:922-931. © 2011 Wiley Periodicals, Inc.
authors: U.S. Department of Energy Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan 48824, USA.
authors: Goutami
authors: G
authors: Banerjee
authors: Suzana
authors: S
authors: Car
authors: Tongjun
authors: T
authors: Liu
authors: Daniel L
authors: DL
authors: Williams
authors: Sarynna López
authors: SL
authors: Meza
authors: Jonathan D
authors: JD
authors: Walton
authors: David B
authors: DB
authors: Hodge
mesh_terms: D056804:Biofuels; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects; D018547:Plant Stems / Q000187:drug effects; D011096:Polygalacturonase / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology*; D014994:Xylose / Q000096:biosynthesis; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; C036909:lignocellulose; D000431:Ethanol; D012972:Sodium Hydroxide; D008031:Lignin; D014994:Xylose; D006861:Hydrogen Peroxide; D011096:Polygalacturonase; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose