title: Evaluation of cellulases produced from four fungi cultured on furfural residues and microcrystalline cellulose.
abstract: Four fungal strains-Trichoderma viride, Aspergillus niger, Trichoderma koningii, and Trichoderma reesei-were selected for cellulase production using furfural residues and microcrystalline cellulose (MCC) as the substrates. The filter paper activity (FPA) of the supernatant from each fungus was measured, and the performance of the enzymes from different fungal strains was compared. Moreover, the individual activities of the three components of the cellulase system, i.e., β-glucosidase, endoglucanase, and exoglucanase were evaluated. T. koningii showed the highest activity (27.81 FPU/ml) on furfural residues, while T. viride showed an activity of 21.61 FPU/ml on MCC. The FPA of the crude enzyme supernatant from T. koningii was 30% higher on furfural residues than on MCC. T. koningii and T. viride exhibited high stability and productivity and were chosen for cellulases production. The crystallinity index (CrI) of the furfural residues varied after digested by the fungi. The results indicated differences in the functioning of the cellulase system from each fungus. In the case of T. koningii, T. reesei and T. viride, furfural residues supported a better environment for cellulase production than MCC. Moreover, the CrI of the furfural residues decreased, indicating that this material was largely digested by the fungi. Thus, our results suggest that it may be possible to use the cellulases produced from these fungi for the simultaneous saccharification and fermentation of lignocellulosic materials in ethanol production.
authors: Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing, 100083, China.
authors: Hui-Qin
authors: HQ
authors: Liu
authors: Yue
authors: Y
authors: Feng
authors: Dan-Qing
authors: DQ
authors: Zhao
authors: Jian-Xin
authors: JX
authors: Jiang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D044602:Cellulases / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005662:Furaldehyde / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D002482:Cellulose; D005662:Furaldehyde; D044602:Cellulases