title: Alkaloid patterns in Leucojum aestivum shoot culture cultivated at temporary immersion conditions.
abstract: The alkaloid patterns in Leucojum aestivum L. shoot culture cultivated at temporary immersion conditions were investigated using gas chromatography-mass spectrometry. 18 alkaloids were identified, and galanthamine, hamayne and lycorine were dominant. The L. aestivum 80 shoot culture, cultivated at temporary immersion conditions, is a prospective biological matrix for obtaining wide range Amaryllidaceae alkaloids, showing valuable biological and pharmacological activities. The temperature of cultivation influenced enzyme activities, catalyzing phenol oxidative coupling of 4'-O-methylnorbelladine and formation of the different groups Amaryllidaceae alkaloids. Decreasing the temperature of cultivation of L. aestivum 80 shoot culture led to activation of para-ortho' phenol oxidative coupling (formation of galanthamine type alkaloids) and inhibited ortho-para' and para-para' phenol oxidative coupling (formation of lycorine and haemanthamine types alkaloids).
authors: Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria.
authors: Ivan
authors: I
authors: Ivanov
authors: Vasil
authors: V
authors: Georgiev
authors: Strahil
authors: S
authors: Berkov
authors: Atanas
authors: A
authors: Pavlov
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000378:metabolism*; D047151:Amaryllidaceae Alkaloids / Q000302:isolation & purification; D018929:Cell Culture Techniques; D005702:Galantamine / Q000096:biosynthesis / Q000302:isolation & purification; D007101:Immersion; D019668:Liliaceae / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism*; D010617:Phenanthridines / Q000302:isolation & purification; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism
chemical_list: D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; D010937:Plant Growth Regulators; C569334:hamayne; D005702:Galantamine; C015330:lycorine