title: Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria.
abstract: We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance gene mecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.
authors: VTT Technical Research Centre of Finland, Kaitoväylä 1, P.O. Box 1100, 90571, Oulu, Finland. ralph.liedert@vtt.fi
authors: Ralph
authors: R
authors: Liedert
authors: Lotta K
authors: LK
authors: Amundsen
authors: Ari
authors: A
authors: Hokkanen
authors: Minna
authors: M
authors: Mäki
authors: Anne
authors: A
authors: Aittakorpi
authors: Mikko
authors: M
authors: Pakanen
authors: James R
authors: JR
authors: Scherer
authors: Richard A
authors: RA
authors: Mathies
authors: Marika
authors: M
authors: Kurkinen
authors: Sanna
authors: S
authors: Uusitalo
authors: Leena
authors: L
authors: Hakalahti
authors: Tarja K
authors: TK
authors: Nevanen
authors: Harri
authors: H
authors: Siitari
authors: Hans
authors: H
authors: Söderlund
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C487495:MecA protein, Staphylococcus epidermidis; D019904:Polymethyl Methacrylate