title: Development of effective nanobiocatalytic systems through the immobilization of hydrolases on functionalized carbon-based nanomaterials.
abstract: In this study we report the use of functionalized carbon-based nanomaterials, such as amine-functionalized graphene oxide (GO) and multi-walled carbon nanotubes (CNTs), as effective immobilization supports for various lipases and esterases of industrial interest. Structural and biochemical characterization have revealed that the curvature of the nanomaterial affect the immobilization yield, the catalytic behavior and the secondary structure of enzymes. Infrared spectroscopy study indicates that the catalytic behavior of the immobilized enzymes is correlated with their α-helical content. Hydrolases exhibit higher esterification activity (up to 20-fold) when immobilized on CNTs compared to GO. The covalently immobilized enzymes exhibited comparable or even higher activity compared to the physically adsorbed ones, while they presented higher operational stability. The enhanced catalytic behavior observed for most of the hydrolases covalently immobilized on amine-functionalized CNTs indicate that these functionalized nanomaterials are suitable for the development of efficient nanobiocatalytic systems.
authors: Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece.
authors: Ioannis V
authors: IV
authors: Pavlidis
authors: Torge
authors: T
authors: Vorhaben
authors: Theodoros
authors: T
authors: Tsoufis
authors: Petra
authors: P
authors: Rudolf
authors: Uwe T
authors: UT
authors: Bornscheuer
authors: Dimitrios
authors: D
authors: Gournis
authors: Haralambos
authors: H
authors: Stamatis
mesh_terms: D000588:Amines / Q000737:chemistry*; D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism; D004951:Esterification; D006108:Graphite / Q000737:chemistry; D006867:Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D017433:Protein Structure, Secondary; D059027:Recycling; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006108:Graphite; D006867:Hydrolases; D004950:Esterases; D008049:Lipase