title: A novel colorimetric determination of reduced glutathione in A549 cells based on Fe3O4 magnetic nanoparticles as peroxidase mimetics.
abstract: In this paper, a novel and simple colorimetric method for the determination of reduced glutathione (GSH) based on Fe(3)O(4) magnetic nanoparticles (MNPs) as peroxidase mimetics was developed. The Fe(3)O(4) MNPs prepared via a coprecipitation method, which possess intrinsic peroxidase-like activity, were used as a catalyst in the color development reaction of a peroxidase substrate 2,2'-azino-bis(3-thylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS) and H(2)O(2). The existence of GSH can consume H(2)O(2) and cause a color change of the reaction system which can be detected by the naked eye. Accordingly, the GSH can be detected by measuring the wastage of H(2)O(2). A good linear relationship was obtained from 3.0 to 30.0 μM for GSH. Good recoveries ranging from 96.7 to 107% were obtained. Furthermore, it was used to detect GSH in A549 cells.
authors: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000, China.
authors: Yanhua
authors: Y
authors: Ma
authors: Zhenyang
authors: Z
authors: Zhang
authors: Cuiling
authors: C
authors: Ren
authors: Guoyun
authors: G
authors: Liu
authors: Xingguo
authors: X
authors: Chen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry*; D002384:Catalysis; D003124:Colorimetry; D004396:Coloring Agents; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D006397:Hematinics / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008175:Lung Neoplasms / Q000378:metabolism; D008280:Magnetics; D053768:Metal Nanoparticles; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D004396:Coloring Agents; D006397:Hematinics; D006861:Hydrogen Peroxide; D009195:Peroxidase; D005978:Glutathione; D052203:Ferrosoferric Oxide