title: Disodium cromoglycate attenuates hypoxia induced enlargement of end-expiratory lung volume in rats.
abstract: Mechanism responsible for the enlargement of end-expiratory lung volume (EELV) induced by chronic hypoxia remains unclear. The fact that the increase in EELV persists after return to normoxia suggests involvement of morphological changes. Because hypoxia has been also shown to activate lung mast cells, we speculated that they could play in the mechanism increasing EELV similar role as in vessel remodeling in hypoxic pulmonary hypertension (HPH). We, therefore, tested an effect of mast cells degranulation blocker disodium cromoglycate (DSCG) on hypoxia induced EELV enlargement. Ventilatory parameters, EELV and right to left heart weight ratio (RV/LV+S) were measured in male Wistar rats. The experimental group (H+DSCG) was exposed to 3 weeks of normobaric hypoxia and treated with DSCG during the first four days of hypoxia, control group was exposed to hypoxia only (H), two others were kept in normoxia as non-treated (N) and treated (N+DSCG) groups. DSCG treatment significantly attenuated the EELV enlargement (H+DSCG = 6.1+/-0.8; H = 9.2+/-0.9; ml +/-SE) together with the increase in minute ventilation (H + DSCG = 190+/-8; H = 273 +/- 10; ml/min +/- SE) and RV/LV + S (H + DSCG = 0.39 +/- 0.03; H = 0.50 +/- 0.06).
authors: Department of Pathophysiology, Second Faculty of Medicine, Charles University in Prague, Praha, Czech Republic. hana.maxova@lfmotol.cuni.cz
authors: H
authors: H
authors: Maxová
authors: A
authors: A
authors: Hezinová
authors: M
authors: M
authors: Vízek
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology*; D045853:Exhalation / Q000187:drug effects*; D000860:Hypoxia / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology*; D008168:Lung / Q000187:drug effects / Q000503:physiopathology*; D008176:Lung Volume Measurements; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics / Q000187:drug effects*; D013990:Tidal Volume / Q000187:drug effects*
chemical_list: D018927:Anti-Asthmatic Agents; D004205:Cromolyn Sodium