title: Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells.
abstract: We aimed to establish a culture system of human pluripotent stem cells (hPSCs), such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), free from xenogeneic proteins, Matrigel(™) and conditioned medium of mouse embryonic fibroblasts.  The conditioned culture medium consisted of mesenchymal stem cells derived from human bone marrow. We examined surface properties suitable for hPSC culture by using self-assembled monolayers (SAMs) of alkanethiols with four different functional groups: CH(3), OH, COOH and NH(2).  hPSCs neither adhered nor proliferated on surfaces with a water contact angle higher than 40°. Based on this finding, the contact angle of a polystyrene (PSt) culture dish was reduced to less than 40°, and COOH and OH groups were introduced to its surface by oxygen plasma treatment,  making the PSt dish suitable for hPSC culture. This combination of a PSt dish treated with oxygen plasma treatment and conditioned medium of mesenchymal stem cells achieved a long-term maintenance of hPSCs without differentiation.
authors: Department of Rgenerative Materials, Institute for Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Shogo-in, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Yusuke
authors: Y
authors: Ueda
authors: Satoshi
authors: S
authors: Fujita
authors: Tatsuya
authors: T
authors: Nishigaki
authors: Yusuke
authors: Y
authors: Arima
authors: Hiroo
authors: H
authors: Iwata
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003094:Collagen / Q000302:isolation & purification; D017077:Culture Media, Conditioned / Q000737:chemistry*; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007797:Laminin / Q000302:isolation & purification; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D058626:Plasma Gases / Q000737:chemistry; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011509:Proteoglycans / Q000302:isolation & purification; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D058626:Plasma Gases; D011509:Proteoglycans; D013438:Sulfhydryl Compounds; C059485:matrigel; D003094:Collagen