title: Crystal structure of malonyl CoA-Acyl carrier protein transacylase from Xanthomanous oryzae pv. oryzae and its proposed binding with ACP.
abstract: Xanthomonas oryzae pv. oryzae (Xoo) is a plant bacterial pathogen that causes bacterial blight (BB) disease, resulting in serious production losses of rice. The crystal structure of malonyl CoA-acyl carrier protein transacylase (XoMCAT), encoded by the gene fabD (Xoo0880) from Xoo, was determined at 2.3 Å resolution in complex with N-cyclohexyl-2-aminoethansulfonic acid. Malonyl CoA-acyl carrier protein transacylase transfers malonyl group from malonyl CoA to acyl carrier protein (ACP). The transacylation step is essential in fatty acid synthesis. Based on the rationale, XoMCAT has been considered as a target for antibacterial agents against BB. Protein-protein interaction between XoMCAT and ACP was also extensively investigated using computational docking, and the proposed model revealed that ACP bound to the cleft between two XoMCAT subdomains.
authors: Department of Advanced Technology Fusion, Konkuk University, Seoul 143-701, Korea.
authors: Sampath
authors: S
authors: Natarajan
authors: Jin-Kwang
authors: JK
authors: Kim
authors: Tae-Kyun
authors: TK
authors: Jung
authors: Thanh Thi Ngoc
authors: TT
authors: Doan
authors: Ho-Phuong-Thuy
authors: HP
authors: Ngo
authors: Myoung-Ki
authors: MK
authors: Hong
authors: Seunghwan
authors: S
authors: Kim
authors: Viet Pham
authors: VP
authors: Tan
authors: Seok Joon
authors: SJ
authors: Ahn
authors: Sang Hee
authors: SH
authors: Lee
authors: Yesun
authors: Y
authors: Han
authors: Yeh-Jin
authors: YJ
authors: Ahn
authors: Lin-Woo
authors: LW
authors: Kang
mesh_terms: D000213:Acyl Carrier Protein / Q000378:metabolism*; D051080:Acyl-Carrier Protein S-Malonyltransferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D008969:Molecular Sequence Data; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D008316:Malonyl Coenzyme A; D051080:Acyl-Carrier Protein S-Malonyltransferase