title: Inhibition of tumor growth by antibody to ADAMTS1 in mouse xenografts of breast cancer.
abstract: BACKGROUND
  A disintegrin and metalloproteinase with thrombospondin motifs-1 (ADAMTS1), a member of the ADAMTS family of proteases, is involved in the shedding of epidermal growth factor (EGF)-like ligands such as amphiregulin, which activate the EGF receptor. Since ADAMTS1 has been implicated in aggressive breast carcinogenesis, we examined potential antitumor effects of antibody to ADAMTS1 in a mouse model of breast cancer.
  
  
  METHODS
  BALB/c female mice were inoculated with syngenic 4T1 breast cancer cells and treated with anti-ADAMTS1 antibody or control IgG. Tumor volume and weight were evaluated.
  
  
  RESULTS
  Mouse 4T1 cells expressed ADAMTS1 and its substrates amphiregulin and heparin-binding EGF. Treatment with antibody to ADAMTS1 inhibited tumor growth without any adverse effects.
  
  
  CONCLUSIONS
  ADAMTS1 could be a promising molecular target for immunotherapy of breast cancer.
authors: Division of Breast and Endocrine Surgery, Department of Surgery, Nihon University School of Medicine, Itabashi-ku, Tokyo 173-8610, Japan.
authors: Tomohiro
authors: T
authors: Hirano
authors: Kunitaka
authors: K
authors: Hirose
authors: Kenichi
authors: K
authors: Sakurai
authors: Makoto
authors: M
authors: Makishima
authors: Kenji
authors: K
authors: Sasaki
authors: Sadao
authors: S
authors: Amano
mesh_terms: D051722:ADAM Proteins / Q000276:immunology* / Q000378:metabolism; D000071097:ADAMTS1 Protein; D066258:Amphiregulin; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D015152:Blotting, Northern; D066255:EGF Family of Proteins; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: C587848:AREG protein, human; D066258:Amphiregulin; D000911:Antibodies, Monoclonal; C587849:Areg protein, mouse; D066255:EGF Family of Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D066246:ErbB Receptors; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486323:Adamts1 protein, mouse