title: In vitro activities of natural products against oral Candida isolates from denture wearers.
abstract: BACKGROUND
  Candida-associated denture stomatitis is a frequent infectious disease. Treatment of this oral condition is difficult because failures and recurrences are common. The aim of this study was to test the in vitro antifungal activity of pure constituents of essentials oils.
  
  
  METHODS
  Eight terpenic derivatives (carvacrol, farnesol, geraniol, linalool, menthol, menthone, terpinen-4-ol, and α-terpineol), a phenylpropanoid (eugenol), a phenethyl alcohol (tyrosol) and fluconazole were evaluated against 38 Candida isolated from denture-wearers and 10 collection Candida strains by the CLSI M27-A3 broth microdilution method.
  
  
  RESULTS
  Almost all the tested compounds showed antifungal activity with MIC ranges of 0.03-0.25% for eugenol and linalool, 0.03-0.12% for geraniol, 0.06-0.5% for menthol, α-terpineol and terpinen-4-ol, 0.03-0.5% for carvacrol, and 0.06-4% for menthone. These compounds, with the exception of farnesol, menthone and tyrosol, showed important in vitro activities against the fluconazole-resistant and susceptible-dose dependent Candida isolates.
  
  
  CONCLUSIONS
  Carvacrol, eugenol, geraniol, linalool and terpinen-4-ol were very active in vitro against oral Candida isolates. Their fungistatic and fungicidal activities might convert them into promising alternatives for the topic treatment of oral candidiasis and denture stomatitis.
authors: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Bilbao, Spain.
authors: Cristina
authors: C
authors: Marcos-Arias
authors: Elena
authors: E
authors: Eraso
authors: Lucila
authors: L
authors: Madariaga
authors: Guillermo
authors: G
authors: Quindós
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology*; D019554:Dental Restoration Wear / Q000009:adverse effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D013282:Stomatitis, Denture / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D001688:Biological Products; D009822:Oils, Volatile