title: Fluoride-treated bio-resorbable synthetic nonceramic [corrected] hydroxyapatite promotes proliferation and differentiation of human osteoblastic MG-63 cells.
abstract: When resorbable hydroxyapatite (HA) granules, which are used as a bone supplement material, were treated in neutral 4% sodium fluoride (NaF) solution, formation of a reactant resembling calcium fluoride was observed on the surface of the granules. Immediate and slow release of fluoride from fluoridated HA (HA+F) granules was observed after immersion in culture fluid, and the concentration increased over time to 1.25 ± 0.05 ppm F at 0.5 hours, 1.57 ± 0.12 ppm F at 24 hours, and 1.73 ± 0.15 ppm F at 48 hours. On invasion assay, migration of human osteoblast-like MG-63 cells exposed to the released fluoride was confirmed in comparison to the cells incubated with a nonfluoridated control sample (P < .01). In addition, fluoride added to the medium increased MG-63 cell proliferation in a manner dependent on fluoride concentrations up to 2.0 ppm (P < .05). At 5.0 ppm, however, fluoride significantly inhibited cell proliferation (P < .005). Activity of the osteogenic differentiation marker, alkaline phosphatase (ALP), also increased with fluoride after exposure for 1 week, increasing significantly at 1.0 ppm (P < .05). The promotion of MG-63 cell migration and proliferation, as well as increased ALP activity, suggested that fluoride released from the surface of resorbable HA granules, which were fluoridated by prior treatment with neutral 4% NaF solution, can provide a superb method to supply fluoride and promote osteogenic cell differentiation.
authors: Department of Health Science, Kanagawa Dental College, Yokosuka, Japan.
authors: Motofumi
authors: M
authors: Ohno
authors: Kazunari
authors: K
authors: Kimoto
authors: Toshihisa
authors: T
authors: Toyoda
authors: Kazushige
authors: K
authors: Kawata
authors: Hirohisa
authors: H
authors: Arakawa
mesh_terms: D020341:Absorbable Implants; D000469:Alkaline Phosphatase / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D005459:Fluorides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology; D017736:Ion-Selective Electrodes; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D012969:Sodium Fluoride / Q000737:chemistry; D013997:Time Factors
chemical_list: D003470:Culture Media; D003692:Delayed-Action Preparations; D006882:Hydroxyapatites; C116586:fluorohydroxyapatite; D012969:Sodium Fluoride; D017886:Durapatite; D000469:Alkaline Phosphatase; D005459:Fluorides