title: Nine microsatellite markers for the Australian side-necked turtle Chelodina expansa (Chelidae) and cross species amplifications.
abstract: Nine microsatellite DNA loci for the Australian broad-shelled freshwater turtle (Chelodina expansa) are presented. Markers were tailed with 20-mer oligonucleotides for use in four-colour fluorescent multiplex PCRs. The markers show high allelic richness (mean N(A)=10.9, range 2-38) and expected heretozygosity (mean H(E)=0.643; range 0.161-0.963) indicating that they will be valuable for population genetics studies in C. expansa. Cross-species amplification in three Australian freshwater turtle species further highlights the potential utility of these markers, particularly in the side-neck species C. longicollis and C. rugosa.
authors: Australian Rivers Institute, School of Environment, Griffith University, Nathan 4111, Queensland, Australia. o.baggiano@griffith.edu.au
authors: Olivier
authors: O
authors: Baggiano
authors: Daniel James
authors: DJ
authors: Schmidt
authors: Jane Margaret
authors: JM
authors: Hughes
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics; D011793:Queensland; D013045:Species Specificity; D014426:Turtles / Q000235:genetics*
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides