title: Glucans from alkaline extract of a hybrid mushroom (backcross mating between PfloVv12 and Volvariella volvacea): structural characterization and study of immunoenhancing and antioxidant properties.
abstract: Two different glucans (water-soluble PS-I, water-insoluble PS-II) were isolated from the alkaline extract of the fruit bodies of hybrid mushroom. PS-I was found to consist of only (1→6)-linked β-D-glucopyranose. PS-II was composed of terminal, (1→3,4)-linked, and (1→3)-linked β-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:1. PS-I showed macrophages, splenocytes, and thymocytes activation as well as antioxidant property. On the basis of sugar analysis, methylation analysis, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these glucans were established as:
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Ramsankar
authors: R
authors: Sarkar
authors: Chanchal K
authors: CK
authors: Nandan
authors: Sanjoy K
authors: SK
authors: Bhunia
authors: Swatilekha
authors: S
authors: Maiti
authors: Tapas K
authors: TK
authors: Maiti
authors: Samir R
authors: SR
authors: Sikdar
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006824:Hybridization, Genetic; D006863:Hydrogen-Ion Concentration; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010851:Picrates / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics*; D012995:Solubility; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D055367:Volvariella / Q000737:chemistry* / Q000235:genetics*; D014867:Water / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D000975:Antioxidants; D001713:Biphenyl Compounds; D005936:Glucans; D010851:Picrates; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl