title: Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies.
abstract: The technique of single muscle-fiber cultures has already proven valuable in extending knowledge of myogenesis, stem cell heterogeneity, the stem cell niche in skeletal muscle, and satellite cell activation. This report reviews the background of the model and applications, and details the procedures of muscle dissection, fiber digestion and isolation, cleaning the fiber preparation, plating fibers, and extensions of the technique for studying activation from stable quiescence of satellite cells, mRNA expression by in situ hybridization and regulation of satellite cell activation in zebrafish muscle by nitric oxide, hepatocyte growth factor.
authors: Department of Biological Sciences, Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, MB, Canada. judy_anderson@umanitoba.ca
authors: Judy E
authors: JE
authors: Anderson
authors: Ashley C
authors: AC
authors: Wozniak
authors: Wataru
authors: W
authors: Mizunoya
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D017403:In Situ Hybridization / Q000379:methods; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009569:Nitric Oxide / Q000494:pharmacology; D061251:Primary Cell Culture / Q000379:methods*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015027:Zebrafish
chemical_list: D009569:Nitric Oxide; D017228:Hepatocyte Growth Factor; D017364:Collagenases