title: Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide-induced leaf cell death in rice.
abstract: Nitric oxide (NO) is a key redox-active, small molecule involved in various aspects of plant growth and development. Here, we report the identification of an NO accumulation mutant, nitric oxide excess1 (noe1), in rice (Oryza sativa), the isolation of the corresponding gene, and the analysis of its role in NO-mediated leaf cell death. Map-based cloning revealed that NOE1 encoded a rice catalase, OsCATC. Furthermore, noe1 resulted in an increase of hydrogen peroxide (H(2)O(2)) in the leaves, which consequently promoted NO production via the activation of nitrate reductase. The removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants, implicating NO as an important endogenous mediator of H(2)O(2)-induced leaf cell death. Reduction of intracellular S-nitrosothiol (SNO) levels, generated by overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1), which regulates global levels of protein S-nitrosylation, alleviated leaf cell death in noe1 plants. Thus, S-nitrosylation was also involved in light-dependent leaf cell death in noe1. Utilizing the biotin-switch assay, nanoliquid chromatography, and tandem mass spectrometry, S-nitrosylated proteins were identified in both wild-type and noe1 plants. NO targets identified only in noe1 plants included glyceraldehyde 3-phosphate dehydrogenase and thioredoxin, which have been reported to be involved in S-nitrosylation-regulated cell death in animals. Collectively, our data suggest that both NO and SNOs are important mediators in the process of H(2)O(2)-induced leaf cell death in rice.
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Chinese Academy of Sciences, Beijing 100101, China.
authors: Aihong
authors: A
authors: Lin
authors: Yiqin
authors: Y
authors: Wang
authors: Jiuyou
authors: J
authors: Tang
authors: Peng
authors: P
authors: Xue
authors: Chunlai
authors: C
authors: Li
authors: Linchuan
authors: L
authors: Liu
authors: Bin
authors: B
authors: Hu
authors: Fuquan
authors: F
authors: Yang
authors: Gary J
authors: GJ
authors: Loake
authors: Chengcai
authors: C
authors: Chu
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism*; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D026403:S-Nitrosothiols / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D026403:S-Nitrosothiols; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D002374:Catalase; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent