title: Survivin deficiency leads to imparalization of cytokinesis in cancer cells.
abstract: BACKGROUND
  Survivin has been implicated in cancer progression and is known to be over-expressed in a variety of human malignancies. Positive regulation of survivin expression provides a connecting link between cell cycle and tumorigenesis or perhaps tumour maintenance.
  
  
  METHODS
  An experiment was designed to analyse survivin expression in cell lines (MCF 7, Zr751, A549, HepG2) using SDS-PAGE, Western blots, RT-PCR, AGE and heamatoxylin-eosin staining were done.
  
  
  RESULTS
  SDS-PAGE revealed the presence of 16.5 kDa protein. Subsequent western Blot and cytological analysis showed down-regulation of survivin expression in cancer cells.
  
  
  CONCLUSIONS
  Therefore, the study allows the conclusion that survivin is essential for proper chromosome segregation and cytokinesis. It seems reasonable to suspect that abnormal expression or function of survivin might contribute to multinucleated and apoptotic conditions.
authors: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, India.
authors: Raju
authors: R
authors: Vivek
authors: Soundarapandian
authors: S
authors: Kannan
authors: Shanmugam
authors: S
authors: Achiraman
authors: Ramasamy
authors: R
authors: Thirumurugan
authors: Devaraj Sankar
authors: DS
authors: Ganesh
authors: Muthukalingan
authors: M
authors: Krishnan
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048749:Cytokinesis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000172:deficiency* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D000077022:Survivin