title: Enhancement of rutin in Fagopyrum esculentum hairy root cultures by the Arabidopsis transcription factor AtMYB12.
abstract: Common buckwheat (Fagopyrum esculentum Moench) is rich in phenolic compounds and may be useful for the treatment of metabolic syndrome in humans. To improve the production of rutin in buckwheat, we overexpressed the flavonol-specific transcription factor, AtMYB12 using Agrobacterium rhizogenes into hairy root culture systems. This induced the expression of flavonoid biosynthetic genes encoding phenylalanine ammonia lyase, cinnamate 4-hydroxylase, 4-coumarate:CoA ligase, chalcone synthase, chalcone isomerase, flavone 3-hydroxylase, flavonoid 3'-hydroxylase, and flavonol synthase. This led to the accumulation of rutin in buckwheat hairy roots up to 0.9 mg/g dry wt. PAP1 expression, however, did not correlate with the production of rutin.
authors: Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-754, Korea.
authors: Nam Il
authors: NI
authors: Park
authors: Xiaohua
authors: X
authors: Li
authors: Aye Aye
authors: AA
authors: Thwe
authors: Sook Young
authors: SY
authors: Lee
authors: Su Gwan
authors: SG
authors: Kim
authors: Qi
authors: Q
authors: Wu
authors: Sang Un
authors: SU
authors: Park
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D018929:Cell Culture Techniques / Q000379:methods; D019612:Fagopyrum / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D060847:Metabolic Engineering / Q000379:methods*; D000073718:Pancreatitis-Associated Proteins; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D012431:Rutin / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C504539:MYB12 protein, Arabidopsis; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D011994:Recombinant Proteins; D014157:Transcription Factors; D012431:Rutin