title: Metabolite analysis of Cannabis sativa L. by NMR spectroscopy.
abstract: NMR-based metabolomics is an analytical platform, which has been used to classify and analyze Cannabis sativa L. cell suspension cultures and plants. Diverse groups of primary and secondary metabolites were identified by comparing NMR data with reference compounds and/or by structure elucidation using ¹H-NMR, J-resolved, ¹H-¹H COSY, and ¹H-¹³C HMBC spectroscopy. The direct extraction and the extraction by indirect fractionation are two suitable methods for the C. sativa sample preparation. Quantitative analyses could be performed without requiring fractionation or isolation procedures.
authors: Institute of Biological Chemistry, Washington State University, Pullman, WA, USA. i.flores.sanchez@chem.leidenuniv.nl
authors: Isvett Josefina
authors: IJ
authors: Flores-Sanchez
authors: Young Hae
authors: YH
authors: Choi
authors: Robert
authors: R
authors: Verpoorte
mesh_terms: D002188:Cannabis / Q000378:metabolism*; D018929:Cell Culture Techniques; D005591:Chemical Fractionation; D035264:Flowers / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D015999:Multivariate Analysis; D010936:Plant Extracts / Q000302:isolation & purification; D018515:Plant Leaves / Q000378:metabolism*; D025341:Principal Component Analysis
chemical_list: D010936:Plant Extracts