title: Comparison of the cytogenotoxicity induced by five different dental alloys using four in vitro assays.
abstract: The purpose of present investigation was to compare the cyto-genotoxicity induced by five dental alloys in vitro. The cyto-genotoxicity induced by five dental alloy extracts on human B lymphoblast cells was assessed with neutral red uptake (NRU) , CCK-8, comet and micronucleus (CBMN) assays in vitro, respectively. The results of in vitro comet and CBMN assays indicated that DNA damage (% tail DNA) and micronucleus frequencies (MNFs) in all exposure groups did not significantly increase, as compared with the control group. However, the results of NRU and CCK-8 assays demonstrated that there were to some extent differences in the cytotoxicity among 5 dental alloy extracts in vitro. The cytotoxicity may be relevant to the Ni and Be ions released in the alloy extract.
authors: Zhejiang University, Stomatology Hospital of Medical College.
authors: Chen
authors: C
authors: Zhihong
authors: Lu
authors: L
authors: Yezhen
authors: Gu
authors: G
authors: Zhiyuan
authors: Yang
authors: Y
authors: Zhongqiao
authors: Li
authors: L
authors: Xiaoxue
authors: Ruan
authors: R
authors: Yifeng
authors: Fang
authors: F
authors: Weikun
authors: He
authors: H
authors: Jiliang
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects*; D001608:Beryllium / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002857:Chromium / Q000633:toxicity; D003035:Cobalt / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D003722:Dental Alloys / Q000633:toxicity*; D006801:Humans; D008422:Materials Testing; D015162:Micronucleus Tests; D009532:Nickel / Q000633:toxicity
chemical_list: D003722:Dental Alloys; D002857:Chromium; D003035:Cobalt; D009532:Nickel; D001608:Beryllium