title: Safe harbours for the integration of new DNA in the human genome.
abstract: Interactions between newly integrated DNA and the host genome limit the reliability and safety of transgene integration for therapeutic cell engineering and other applications. Although targeted gene delivery has made considerable progress, the question of where to insert foreign sequences in the human genome to maximize safety and efficacy has received little attention. In this Opinion article, we discuss 'genomic safe harbours' - chromosomal locations where therapeutic transgenes can integrate and function in a predictable manner without perturbing endogenous gene activity and promoting cancer.
authors: Center for Cell Engineering, Molecular Pharmacology and Chemistry Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA. m-sadelain@ski.mskcc.org
authors: Michel
authors: M
authors: Sadelain
authors: Eirini P
authors: EP
authors: Papapetrou
authors: Frederic D
authors: FD
authors: Bushman
mesh_terms: D000818:Animals; D060846:Cell Engineering; D000229:Dependovirus / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D015894:Genome, Human; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D019713:Receptors, CCR5 / Q000235:genetics; D016662:Virus Integration
chemical_list: C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; D019713:Receptors, CCR5