title: [Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-fLD after immunoaffinity column with online post-column photochemical derivatization].
abstract: OBJECTIVE
  To develop a method for the simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization.
  
  
  METHODS
  Sample was extracted with MeOH: H2O (80:20) and cleaned up by immunoaffinity column. The toxins were separated by reversed-phase HPLC and the mobile phase was consisted of methanol and 0.5% acetic acid solution with gradient elution. The determination was carried out by fluorescence detector after photochemical derivatization.
  
  
  RESULTS
  The detection limits of aflatoxin G2, G1, B2, B1 and ochratoxin A were 0.02, 0.06, 0.015, 0.03 and 0.25 microg x kg(-1), respectively. The recoveries of analytes were from 76.0% to 103% and the relative standard deviations (RSDs) were below 13%.
  
  
  CONCLUSIONS
  The method is a simple, accurate and can be used to determine the contents of aflatoxin B1, B2, G1, G2 and ochratoxin A in G. uralensis simultaneously.
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China. weiriweihao@126.com
authors: Riwei
authors: R
authors: Wei
authors: Xiaoli
authors: X
authors: Yang
authors: Feng
authors: F
authors: Qiu
authors: Meihua
authors: M
authors: Yang
authors: Jieping
authors: J
authors: Qin
mesh_terms: D000348:Aflatoxins / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D009793:Ochratoxins / Q000737:chemistry* / Q000302:isolation & purification; D055668:Photochemical Processes
chemical_list: D000348:Aflatoxins; D009793:Ochratoxins; C025589:ochratoxin A