title: Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme.
abstract: Sargassum fusiforme (Harv.) Setchel is an ingredient of Chinese herbal medicine that has been applied for thousands of years. This study was set up to evaluate the in vivo and in vitro anti-tumor potential of the polysaccharide (SFPS) from S. fusiforme and the immune response in tumor-bearing mice. SFPS was isolated by hot water extraction and ethanol precipitation. The mice inoculated with A549 cells were orally administrated with SFPS at the doses of 100 and 200mg/kg body weight for 28 days. The effects on the growth of tumor, serum TNF-α level, splenocyte proliferation, production of cytokines from peritoneal macrophages in A549-bearing mice were measured. Meanwhile, the cytotoxicity of SFPS on A549 cell line was also studied. Results showed that SFPS could not only significantly inhibit the growth of A549 lung adenocarcinoma in mice, but also remarkably promote IL-1 and TNF-α production from peritoneal macrophages, serum TNF-α level, and splenocytes proliferation in A549-bearing mice. The results indicate that SFPS has anti-tumor properties in vivo and in vitro, and improves the immune response in tumor-bearing mice. It could act as anti-tumor agent with immunomodulatory activity.
authors: Institute of Glycobiological Engineering/Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou 325035, China. xmchen01@163.com
authors: Xiaoming
authors: X
authors: Chen
authors: Wenjian
authors: W
authors: Nie
authors: Guoqing
authors: G
authors: Yu
authors: Yali
authors: Y
authors: Li
authors: Yunshuang
authors: Y
authors: Hu
authors: Jianxin
authors: J
authors: Lu
authors: Liqin
authors: L
authors: Jin
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000473:pathology; D011134:Polysaccharides / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D023041:Xenograft Model Antitumor Assays
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D016207:Cytokines; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha