title: MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers.
abstract: Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients; 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes.
authors: Institute of Experimental Neurology (INSPE), and Department of Neurology, Division of Neuroscience, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy. martinelli.filippo@hsr.it
authors: Filippo
authors: F
authors: Martinelli-Boneschi
authors: Chiara
authors: C
authors: Fenoglio
authors: Paola
authors: P
authors: Brambilla
authors: Melissa
authors: M
authors: Sorosina
authors: Giacomo
authors: G
authors: Giacalone
authors: Federica
authors: F
authors: Esposito
authors: Maria
authors: M
authors: Serpente
authors: Claudia
authors: C
authors: Cantoni
authors: Elisa
authors: E
authors: Ridolfi
authors: Mariaemma
authors: M
authors: Rodegher
authors: Lucia
authors: L
authors: Moiola
authors: Bruno
authors: B
authors: Colombo
authors: Milena
authors: M
authors: De Riz
authors: Vittorio
authors: V
authors: Martinelli
authors: Elio
authors: E
authors: Scarpini
authors: Giancarlo
authors: G
authors: Comi
authors: Daniela
authors: D
authors: Galimberti
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger