title: Isolation and characterization of microsatellite loci in a clonal herb, Cardamine leucantha (Brassicaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to analyze the clonal diversity and population genetic structure of a clonal plant, Cardamine leucantha.
  
  
  RESULTS
  Microsatellite loci were isolated by using compound simple sequence repeat (SSR) primers, resulting in 25 pairs including 14 useful markers. Samples from 33, 20, and 17 ramets in three populations were identified as belonging to 23, 18, and 17 genotypic individuals, respectively.
  
  
  CONCLUSIONS
  These SSR markers will be used for identifying genets and evaluating genetic diversity of the clonal plant C. leucantha.
authors: Center for Ecological Research, Kyoto University, Hirano, Otsu, Japan. akiwako@ecology.kyoto-u.ac.jp
authors: Kiwako S
authors: KS
authors: Araki
authors: Shingo
authors: S
authors: Kaneko
authors: Yuji
authors: Y
authors: Isagi
authors: Hiroshi
authors: H
authors: Kudoh
mesh_terms: D000483:Alleles; D031217:Cardamine / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D056426:Genetic Loci / Q000235:genetics*; D006579:Heterozygote; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D018744:DNA, Plant