title: [Effects of sodium on rat osteoblast and the role of epithelial sodium channel].
abstract: OBJECTIVE
  To study the effects of sodium on rat osteoblast function and explore the role of epithelial sodium channel (ENaC) in such effects.
  
  
  METHODS
  The proliferation and differentiation of rat osteoblasts were evaluated following treatment with 1×10(-4) mol/L to 1 mol/L Na(+). The mRNA expressions of the osteogenic genes and ENaC-α gene in the treated cells were assessed using RT-PCR.
  
  
  RESULTS
  Within the concentration of 1×10(-4) mol/L to 1 mol/L, Na(+) showed a two-way effect on the osteoblasts: low-concentration Na(+) (1×10(-4) mol/L) significantly promoted osteoblast differen- tiation, while at higher concentrations (0.5 and 1 mol/L), Na(+) produced an opposite effect. Sodium did not significantly affect osteoblast proliferation. Low-concentration Na(+) significantly increased the transcription of Cbfa1, OPN and OC, while high concentrations of Na(+) decreased their transcription. Low-concentration Na(+) also enhanced the mRNA expression of ENaC-α, but high-concentration Na(+) treatment lowered ENaC-α mRNA expression.
  
  
  CONCLUSIONS
  Na(+) displays a direct dose-related effect on osteoblasts by affecting its differentiation, osteogenic gene expression profile, and ENaC-α gene expression, suggesting the involvement of ENaC in Na(+)-mediated functional modulation of rat osteoblasts.
authors: Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, School of Life Science and Biopharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Li
authors: L
authors: Lu
authors: Liang
authors: L
authors: Wu
authors: Jun
authors: J
authors: Chen
authors: Xiao-hui
authors: XH
authors: Lin
authors: Chao
authors: C
authors: Wan
authors: Qing-nan
authors: QN
authors: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D053503:Epithelial Sodium Channels / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D053503:Epithelial Sodium Channels; C440894:Scnn1a protein, rat; D012965:Sodium Chloride