title: Alveolar macrophage dynamics in murine lung regeneration.
abstract: In most mammalian species, the removal of one lung results in dramatic compensatory growth of the remaining lung. To investigate the contribution of alveolar macrophages (AMs) to murine post-pneumonectomy lung growth, we studied bronchoalveolar lavage (BAL)-derived AM on 3, 7, 14 and 21 days after left pneumonectomy. BAL demonstrated a 3.0-fold increase in AM (CD45(+), CD11b(-), CD11c(+), F4/80(+), Gr-1(-)) by 14 days after pneumonectomy. Cell cycle flow cytometry of the BAL-derived cells demonstrated an increase in S + G2 phase cells on days 3 (11.3 ± 2.7%) and 7 (12.1 ± 1.8%) after pneumonectomy. Correspondingly, AM demonstrated increased expression of VEGFR1 and MHC class II between days 3 and 14 after pneumonectomy. To investigate the potential contribution of peripheral blood cells to this AM population, parabiotic mice (wild-type/GFP) underwent left pneumonectomy. Analysis of GFP(+) cells in the post-pneumonectomy lung demonstrated that by day 14, less than 1% of the AM population were derived from the peripheral blood. Finally, AM gene transcription demonstrated a significant shift from decreased transcription of angiogenesis-related genes on day 3 to increased transcription on day 7 after pneumonectomy. The increased number of locally proliferating AM, combined with their growth-related gene transcription, suggests that AM actively participate in compensatory lung growth.
authors: Laboratory of Adaptive and Regenerative Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors: Kenji
authors: K
authors: Chamoto
authors: Barry C
authors: BC
authors: Gibney
authors: Maximilian
authors: M
authors: Ackermann
authors: Grace S
authors: GS
authors: Lee
authors: Miao
authors: M
authors: Lin
authors: Moritz A
authors: MA
authors: Konerding
authors: Akira
authors: A
authors: Tsuda
authors: Steven J
authors: SJ
authors: Mentzer
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage; D002452:Cell Count; D015870:Gene Expression; D005802:Genes, MHC Class II; D008168:Lung / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000235:genetics; D011013:Pneumonectomy / Q000379:methods; D012038:Regeneration; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D040281:Vascular Endothelial Growth Factor Receptor-1