title: Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii.
abstract: Caspase 3c (MrCasp3c) was sequenced from the freshwater giant prawn Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique. MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa. MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236-367 and 378-468 respectively. The quantitative real time PCR analysis revealed a broad expression of MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition, MrCasp3c was expressed in Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of M. rosenbergii.
authors: Centre for Biotechnology in Agriculture Research, Division of Genetics & Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Jesu
authors: J
authors: Arockiaraj
authors: Sarasvathi
authors: S
authors: Easwvaran
authors: Puganeshwaran
authors: P
authors: Vanaraja
authors: Arun
authors: A
authors: Singh
authors: Rofina Yasmin
authors: RY
authors: Othman
authors: Subha
authors: S
authors: Bhassu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053148:Caspase 3 / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D019223:Densovirinae / Q000276:immunology / Q000502:physiology*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D043143:Hepatopancreas / Q000201:enzymology; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000821:virology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011994:Recombinant Proteins; D053148:Caspase 3