title: Chrysosporium lucknowense C1 arabinofuranosidases are selective in releasing arabinose from either single or double substituted xylose residues in arabinoxylans.
abstract: Two novel arabinofuranosidases, Abn7 and Abf3 from Chrysosporium lucknowense (C1), belonging to the glycoside hydrolase family 43 and 51 were purified and characterized. Abn7 is exclusively able to hydrolyze arabinofuranosyl residues at position O-3 of double substituted xylosyl residues in arabinoxylan-derived oligosaccharides, an activity rarely found thus far. Abf3 is able to release arabinose from position O-2 or O-3 of single substituted xyloses. Both enzymes performed optimal at pH 5.0 and 40°C. Combining Abn7 and Abf3 resulted in a synergistic increase in arabinose release from arabinoxylans. This synergistic effect is due to the action of Abf3 on the remaining arabinose residues at position O-2 on single substituted xylosyl residues resulting from the action of Abn7 on double substituted xylosyl residues. Arabinose release was further increased when an endo-1,4-β-xylanase was present during digestion. The efficiency of these arabinohydrolases from C1 on insoluble arabinoxylan substrates is discussed.
authors: Laboratory of Food Chemistry, Wageningen University, PO Box 8129, 6700 EV Wageningen, The Netherlands.
authors: Laurice
authors: L
authors: Pouvreau
authors: Rob
authors: R
authors: Joosten
authors: Sandra W A
authors: SW
authors: Hinz
authors: Harry
authors: H
authors: Gruppen
authors: Henk A
authors: HA
authors: Schols
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D002912:Chrysosporium / Q000145:classification / Q000201:enzymology*; D003001:Cloning, Molecular; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000737:chemistry / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D014990:Xylans; C085118:arabinoxylan; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase