title: Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample.
abstract: We investigate the effects of 1(st) order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1 μm polystyrene beads up to 50 μm deep in the sample.
authors: SUPA, School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ, UK. maria.dienerowitz@glasgow.ac.uk
authors: Maria
authors: M
authors: Dienerowitz
authors: Graham
authors: G
authors: Gibson
authors: Richard
authors: R
authors: Bowman
authors: Miles
authors: M
authors: Padgett
mesh_terms: D016477:Artifacts; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods*; D007909:Lenses; D052898:Optical Tweezers