title: Caleosin-assembled oil bodies as a potential delivery nanocarrier.
abstract: Encapsulation of hydrophobic agents with nanocarriers is challenging. Therefore, we have sought to use nanoscale artificial oil bodies (NOBs) as an alternative delivery carrier. To constitute NOBs, caleosin (Cal), a structural protein of plant seed oil bodies, was first fused with ZH2 (Cal-ZH2). ZH2 is a bivalent anti-HER2/neu affibody with a high affinity towards the HER2/neu receptor. After overproduction in Escherichia coli, insoluble Cal-ZH2 was isolated and used to assemble NOBs in one step. Consequently, resulting NOBs had a zeta potential around -49 mV and ranged in size from 150 to 200 nm. Upon loading with a hydrophobic fluorescence dye, NOBs were found to be selectively internalized into HER2/neu-positive tumor cells. Further analyses showed that more than 90% cells were invaded by dye-loaded NOBs and the cargo dye was released in cells with time. In addition, the in vitro assay revealed the release of the dye from NOBs in a slow and prolong manner. Overall, these results indicate the potential of Cal-based NOBs as a delivery vehicle.
authors: Department of Medical Laboratory Science and Biotechnology, China Medical University, 91 Hsue-Shih Road, Taichung, 40402, Taiwan. cjchiang@mail.cmu.edu.tw
authors: Chung-Jen
authors: CJ
authors: Chiang
authors: Shen-Chuan
authors: SC
authors: Lin
authors: Li-Jen
authors: LJ
authors: Lin
authors: Chih-Jung
authors: CJ
authors: Chen
authors: Yun-Peng
authors: YP
authors: Chao
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004337:Drug Carriers; D004705:Endocytosis; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D049329:Nanostructures; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D002135:Calcium-Binding Proteins; D004337:Drug Carriers; D005456:Fluorescent Dyes; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C402836:caleosin protein, Sesamum indicum