title: Proteomic analysis of rat microglia establishes a high-confidence reference data set of over 3000 proteins.
abstract: Highly aggressively proliferating immortalized (HAPI) microglial cells have been used as an in vitro model for investigating key microglial functions including inflammatory, neurotoxic, and phagocytic activities. Through the use of offline strong cation-exchange fractionation followed by inline reversed-phase chromatographic separation and tandem mass spectrometric analysis on a hybrid linear ion trap-Orbitrap instrument, the HAPI microglial proteome was characterized to a depth of 3006 unique protein groups. Upon bioinformatic analysis of the HAPI proteome data set, enrichment was observed for processes relevant to microglial function including those associated with immune system response. This study marks the most comprehensive reference data set generated to date for the rat microglial proteome.
authors: Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL 33620, USA.
authors: Harris
authors: H
authors: Bell-Temin
authors: David S
authors: DS
authors: Barber
authors: Ping
authors: P
authors: Zhang
authors: Bin
authors: B
authors: Liu
authors: Stanley M
authors: SM
authors: Stevens
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002852:Chromatography, Ion Exchange; D056148:Chromatography, Reverse-Phase; D019295:Computational Biology; D003593:Cytoplasm / Q000737:chemistry; D030562:Databases, Protein; D017628:Microglia / Q000737:chemistry* / Q000502:physiology; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides; D020543:Proteome