title: Measurement of platelet microparticles.
abstract: Platelet microparticles are submicron vesicles that can support thrombin generation on externalized negatively charged phospholipids. Increased numbers of circulating platelet microparticles have been investigated as the basis of hypercoagulability in a variety of prothrombotic conditions. Measurement of platelet microparticles is not standardized and a number of preanalytic considerations can influence accurate analysis. We describe methodology for light scatter-based flow cytometry as well as impedance-based flow cytometry for the enumeration and characterization of platelet microparticles.
authors: Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. jzwicker@bidmc.harvard.edu
authors: Jeffrey I
authors: JI
authors: Zwicker
authors: Romaric
authors: R
authors: Lacroix
authors: Françoise
authors: F
authors: Dignat-George
authors: Barbara C
authors: BC
authors: Furie
authors: Bruce
authors: B
authors: Furie
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D055252:Cell-Derived Microparticles; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans