title: Heptafluorobutyl chloroformate-based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography.
abstract: Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid-liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.
authors: Laboratory of Analytical Biochemistry, Biology Centre, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic. simek@bclab.eu
authors: Petr
authors: P
authors: Simek
authors: Petr
authors: P
authors: Hušek
authors: Helena
authors: H
authors: Zahradníčková
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D053000:Analytic Sample Preparation Methods; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry*; D005561:Formates / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D012015:Reference Standards; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D005466:Fluorocarbons; D005561:Formates; C570844:heptafluorobutyl chloroformate