title: Solution structure of RING finger-like domain of retinoblastoma-binding protein-6 (RBBP6) suggests it functions as a U-box.
abstract: Retinoblastoma-binding protein-6 (RBBP6) plays a facilitating role, through its RING finger-like domain, in the ubiquitination of p53 by Hdm2 that is suggestive of E4-like activity. Although the presence of eight conserved cysteine residues makes it highly probable that the RING finger-like domain coordinates two zinc ions, analysis of the primary sequence suggests an alternative classification as a member of the U-box family, the members of which do not bind zinc ions. We show here that despite binding two zinc ions, the domain adopts a homodimeric structure highly similar to those of a number of U-boxes. Zinc ions could be replaced by cadmium ions without significantly disrupting the structure or the stability of the domain, although the rate of substitution was an order of magnitude slower than any previous measurement, suggesting that the structure is particularly stable, a conclusion supported by the high thermal stability of the domain. A hallmark of U-box-containing proteins is their association with chaperones, with which they cooperate in eliminating irretrievably unfolded proteins by tagging them for degradation by the proteasome. Using a yeast two-hybrid screen, we show that RBBP6 interacts with chaperones Hsp70 and Hsp40 through its N-terminal ubiquitin-like domain. Taken together with the structural similarities to U-box-containing proteins, our data suggest that RBBP6 plays a role in chaperone-mediated ubiquitination and possibly in protein quality control.
authors: Biotechnology Department, University of the Western Cape, Bellville 7535, South Africa.
authors: Mautin A
authors: MA
authors: Kappo
authors: Eiso
authors: E
authors: Ab
authors: Faqeer
authors: F
authors: Hassem
authors: R Andrew
authors: RA
authors: Atkinson
authors: Andrew
authors: A
authors: Faro
authors: Victor
authors: V
authors: Muleya
authors: Takalani
authors: T
authors: Mulaudzi
authors: John O
authors: JO
authors: Poole
authors: Jean M
authors: JM
authors: McKenzie
authors: Moredreck
authors: M
authors: Chibi
authors: Joanna C
authors: JC
authors: Moolman-Smook
authors: D Jasper G
authors: DJ
authors: Rees
authors: David J R
authors: DJ
authors: Pugh
mesh_terms: D002104:Cadmium / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011485:Protein Binding / Q000502:physiology; D054829:RING Finger Domains; D012441:Saccharomyces cerevisiae / Q000235:genetics; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases; D054875:Ubiquitination / Q000502:physiology; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; C098584:RBBP6 protein, human; D044767:Ubiquitin-Protein Ligases; D015032:Zinc