title: Characterization of a novel type of HMW subunit of glutenin from Australopyrum retrofractum.
abstract: The Triticeae species Australopyrum retrofractum (genome WW) produces a single high molecular weight glutenin subunit (HMW-GS) in its endosperm. However, degenerate PCR amplification of its genome DNA revealed the presence of two related HMW-GS sequences, each consisting of an open reading frame. One of these (Glu-W1-2) has not previously been reported. Here, we sequenced Glu-W1-2 and showed that it encodes the same type of HMW-GS as Glu-W1-1, although its overall product length was much shorter, because the number of certain repetitive motifs was lower in its central region. Both A. retrofractum HMW-GSs have a unique repetitive motif, which differentiates them from other known x- and y-type subunits present in Triticeae species. We suggest that A. retrofractum must have diverged from the main Triticeae lineage prior to the Glu-1 duplication event which led to the evolution of the x- and y-type genes.
authors: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan 250100, PR China.
authors: Fei
authors: F
authors: Li
authors: Xilong
authors: X
authors: Jiang
authors: Yanfeng
authors: Y
authors: Wei
authors: Guangmin
authors: G
authors: Xia
authors: Shuwei
authors: S
authors: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019143:Evolution, Molecular; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021122:Protein Subunits / Q000737:chemistry; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics
chemical_list: D021122:Protein Subunits; D005983:Glutens; C037302:glutenin