title: New vessel formation in peritumoral area of squamous cell carcinoma of the head and neck.
abstract: BACKGROUND
  This study was conducted to investigate the dynamic process of new vessel formation, fundamental for tumor growth and metastasis, in head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  We used immunohistochemistry, confocal laser-scanning microscopy, and reverse transcriptase-polymerase chain reaction to study endothelial cell and concomitant pericyte development with markers CD133, CD34, VEGFR-2, CD31, vWF, and STRO-1 in tumor and peritumoral tissues of 18 patients with HNSCC.
  
  
  RESULTS
  Highly compressed and structurally abnormal vessels with barely any activity of new vessel formation were found in tumor tissue, whereas the adjacent peritumoral tissue vessels showed a normal architecture with tight endothelial cell-pericyte interaction and a high activity of angiogenesis. Endothelial precursor cells expressing CD133/VEGFR-2 could be incorporated into these newly formed vessels, forming cell clusters from which a thin endothelial lining could emanate.
  
  
  CONCLUSIONS
  These data show a high activity of new vessel formation in the peritumoral stroma of HNSCC, with endothelial precursor cells being incorporated into these structures.
authors: Department of Transfusion Medicine, Medical University of Vienna, Vienna, Austria.
authors: David
authors: D
authors: Hollemann
authors: Genya
authors: G
authors: Yanagida
authors: Beate M
authors: BM
authors: Rüger
authors: Csilla
authors: C
authors: Neuchrist
authors: Michael B
authors: MB
authors: Fischer
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000098:blood supply* / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000098:blood supply* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D010455:Peptides / Q000378:metabolism; D020286:Pericytes / Q000378:metabolism / Q000473:pathology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D000954:Antigens, Surface; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C525591:STRO-1 antigen, human; D012313:RNA; D040301:Vascular Endothelial Growth Factor Receptor-2