title: Target molecule imaging on tissue specimens by fluorescent metal nanoprobes.
abstract: In this paper, fluorescence metal nanoshells (FMNs) were synthesized for target molecule detection on tissue specimens by fluorescence imaging method. FMNs were made with 40 nm silica spherical cores and 10 nm silver shells. Ru(bpy)(3)(2+) complexes were encapsulated in the silica cores for fluorescence properties. Avidin molecules were covalently bound on FMNs and formed avidin-Ag complexes could be site-specially conjugated on bone tissue specimens. Fluorescence intensity and lifetime images were recorded on a time-resolved confocal microscope. Imaging measurements showed that the emissions by avidin-FMN complexes could be distinctly isolated as individuals from the cellular backgrounds on lifetime images even when the tissues were stained with additional organic dyes. This observation demonstrates that the metal nanoprobes can be used for single target molecule detection on tissues during fluorescence imaging measurements.
authors: University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201, USA. jian@cfs.biomet.umaryland.edu
authors: Jian
authors: J
authors: Zhang
authors: Yi
authors: Y
authors: Fu
authors: Xuehong
authors: X
authors: Xu
authors: Joseph R
authors: JR
authors: Lakowicz
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry; D001842:Bone and Bones / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006651:Histocytochemistry; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D057145:Nanoshells / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D010316:Particle Size; D012428:Ruthenium / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D009942:Organometallic Compounds; D001360:Avidin; D012834:Silver; D012822:Silicon Dioxide; D012428:Ruthenium