title: Sites of ubiquitin attachment in Saccharomyces cerevisiae.
abstract: Sites of ubiquitin modification have been identified by mass spectrometry based on the increase in molecular mass of a tryptic peptide carrying two additional glycine residues from the ubiquitin moiety. However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae.
authors: Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.
authors: Lea M
authors: LM
authors: Starita
authors: Russell S
authors: RS
authors: Lo
authors: Jimmy K
authors: JK
authors: Eng
authors: Priska D
authors: PD
authors: von Haller
authors: Stanley
authors: S
authors: Fields
mesh_terms: D000345:Affinity Labels / Q000378:metabolism; D001665:Binding Sites; D003545:Cysteine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D000345:Affinity Labels; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D006639:Histidine; D044763:Ubiquitin-Conjugating Enzymes; D003545:Cysteine