title: Differential modifying effects of food additive butylated hydroxytoluene toward radiation and 4-nitro-quinoline 1-oxide-induced genotoxicity in yeast.
abstract: The modifying effect of butylated hydroxytoluene (BHT) on 60Co gamma radiation and 4-nitro-quinoline 1-oxide-induced gene conversion and back mutation frequencies was investigated using diploid yeast Saccharomyces cerevisiae D7. Cells were exposed to 100 or 400 Gy in the presence of 0.025-0.25 mM BHT. BHT exhibited radioprotection and significantly reduced radiation-induced gene conversion and back mutation frequencies as well as cell killing. In another set of experiments, cells were simultaneously treated with 0.025-0.1 mM BHT and 0.5 μM 4-NQO. BHT significantly enhanced 4-NQO-induced gene conversion and back mutation frequencies. BHT post-treatment did not modify radiation-induced genetic events but enhanced 4-NQO-induced back mutation frequencies, indicating its potential to act as a tumor-promoting agent with 4-NQO.
authors: Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Mumbai, India. kba@barc.gov.in
authors: Kshiti B
authors: KB
authors: Anjaria
authors: Nagesh N
authors: NN
authors: Bhat
authors: Kapil B
authors: KB
authors: Shirsath
authors: B
authors: B
authors: Sreedevi
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005503:Food Additives / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays / Q000009:adverse effects*; D005785:Gene Conversion / Q000187:drug effects; D008954:Models, Biological; D009153:Mutagens / Q000494:pharmacology; D059645:Mutation Rate; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects
chemical_list: D005503:Food Additives; D016166:Free Radical Scavengers; D009153:Mutagens; D002084:Butylated Hydroxytoluene; D015112:4-Nitroquinoline-1-oxide; D004121:Dimethyl Sulfoxide