title: kinITC: a new method for obtaining joint thermodynamic and kinetic data by isothermal titration calorimetry.
abstract: Isothermal titration calorimetry (ITC) is the method of choice for obtaining thermodynamic data on a great variety of systems. Here we show that modern ITC apparatus and new processing methods allow researchers to obtain a complete kinetic description of systems more diverse than previously thought, ranging from simple ligand binding to complex RNA folding. We illustrate these new features with a simple case (HIV-1 reverse transcriptase/inhibitor interaction) and with the more complex case of the folding of a riboswitch triggered by the binding of its ligand. The originality of the new kinITC method lies in its ability to dissect, both thermodynamically and kinetically, the two components: primary ligand binding and subsequent RNA folding. We are not aware of another single method that can yield, in a simple way, such deep insight into a composite process. Our study also rationalizes common observations from daily ITC use.
authors: Architecture et Réactivité de l'ARN, Biophysique et Biologie Structurale, Institut de Biologie Moléculaire et Cellulaire du CNRS, Université de Strasbourg, 15, rue René Descartes, 67084 Strasbourg, France.
authors: Dominique
authors: D
authors: Burnouf
authors: Eric
authors: E
authors: Ennifar
authors: Sondes
authors: S
authors: Guedich
authors: Barbara
authors: B
authors: Puffer
authors: Guillaume
authors: G
authors: Hoffmann
authors: Guillaume
authors: G
authors: Bec
authors: François
authors: F
authors: Disdier
authors: Mireille
authors: M
authors: Baltzinger
authors: Philippe
authors: P
authors: Dumas
mesh_terms: D002151:Calorimetry / Q000379:methods*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D015497:HIV-1 / Q000201:enzymology; D007700:Kinetics; D019829:Nevirapine / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors / Q000378:metabolism; D058928:Riboswitch; D013223:Statistics as Topic / Q000379:methods*; D013816:Thermodynamics; D013835:Thiamine Pyrophosphate / Q000378:metabolism
chemical_list: D018894:Reverse Transcriptase Inhibitors; D058928:Riboswitch; D019829:Nevirapine; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D013835:Thiamine Pyrophosphate