title: Fictive locomotion in the adult decerebrate and spinal mouse in vivo.
abstract: Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion; however, it has currently only been possible to evoke fictive locomotion in mice, using neonatal in vitro preparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mouse in vivo using l-3,4-dihydroxyphenylalanine methyl ester hydrochloride (l-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to those that have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.
authors: University of Copenhagen, Department of Neuroscience and Pharmacology, Panum Institute, Bldg 18.5, Blegdamsvej 3, 200 Copenhagen N, Denmark. claire@sund.ku.dk
authors: C F
authors: CF
authors: Meehan
authors: L
authors: L
authors: Grondahl
authors: J B
authors: JB
authors: Nielsen
authors: H
authors: H
authors: Hultborn
mesh_terms: D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D002415:Cats; D003655:Decerebrate State / Q000503:physiopathology*; D004558:Electric Stimulation; D005260:Female; D000882:Haplorhini; D007980:Levodopa / Q000494:pharmacology; D008124:Locomotion / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology*; D009526:Nialamide / Q000494:pharmacology; D010525:Peripheral Nerves / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D011930:Reaction Time / Q000502:physiology; D013116:Spinal Cord / Q000503:physiopathology*
chemical_list: D008996:Monoamine Oxidase Inhibitors; D007980:Levodopa; D006916:5-Hydroxytryptophan; D009526:Nialamide