title: Consequences of alteration in leucine zipper sequence of melittin in its neutralization of lipopolysaccharide-induced proinflammatory response in macrophage cells and interaction with lipopolysaccharide.
abstract: The bee venom antimicrobial peptide, melittin, besides showing versatile activity against microorganisms also neutralizes lipopolysaccharide (LPS)-induced proinflammatory responses in macrophage cells. However, how the amino acid sequence of melittin contributes in its anti-inflammatory properties is mostly unknown. To determine the importance of the leucine zipper sequence of melittin in its neutralization of LPS-induced inflammatory responses in macrophages and interaction with LPS, anti-inflammatory properties of melittin and its three analogues and their interactions with LPS were studied in detail. Two of these analogues, namely melittin Mut-1 (MM-1) and melittin Mut-2 (MM-2), possess leucine to alanine substitutions in the single and double heptadic leucine residue(s) of melittin, respectively, whereas the third analogue is a scrambled peptide (Mel-SCR) that contains the amino acid composition of melittin with minor rearrangement in its leucine zipper sequence. Although MM-1 partly inhibited the production of proinflammatory cytokines in RAW 264.7 and rat primary macrophage cells in the presence of LPS, MM-2 and Mel-SCR were negligibly active. A progressive decrease in interaction of melittin with LPS, aggregation in LPS, and dissociation of LPS aggregates with alteration in the leucine zipper sequence of melittin was observed. Furthermore, with alteration in the leucine zipper sequence of melittin, these analogues failed to exhibit cellular responses associated with neutralization of LPS-induced inflammatory responses in macrophage cells by melittin. The data indicated a probable important role of the leucine zipper sequence of melittin in neutralizing LPS-induced proinflammatory responses in macrophage cells as well as in its interaction with LPS.
authors: Molecular and Structural Biology Division, Sophisticated Analytical Instrument Facility, Central Drug Research Institute, Council of Scientific and Industrial Research, Chattar Manzil Palace, P. O. Box 173, Lucknow 226001, India.
authors: Raghvendra M
authors: RM
authors: Srivastava
authors: Saurabh
authors: S
authors: Srivastava
authors: Manish
authors: M
authors: Singh
authors: Virendra Kumar
authors: VK
authors: Bajpai
authors: Jimut Kanti
authors: JK
authors: Ghosh
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016350:Leucine Zippers / Q000235:genetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008555:Melitten / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D020125:Mutation, Missense; D051381:Rats
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; C440499:lipopolysaccharide, Escherichia coli O111 B4; D008555:Melitten