title: Alteration in lipid and protein profiles of ovarian cancer: similarity to breast cancer.
abstract: This study was undertaken to evaluate protein and lipid profiles of ovarian cancer tissue samples. Twenty-three frozen ovarian cancer samples and 6 adjacent normal samples were analyzed using histology-directed, matrix-assisted laser desorption/ionization mass spectrometry. Sinapinic acid and 2, 5-dihydroxybenzoic acid/α-cyano-4-hydroxycinnamic acid were manually deposited on areas of each tissue section enriched in epithelial cells to identify protein and lipid profiles respectively, and mass spectra were acquired using a matrix-assisted laser desorption/ionization-time of flight instrument. Protein and lipid profiles classify 11 cancer and 3 adjacent normal samples in 100 random test sets with 92.9% median accuracy. Phosphatidylcholines {32:3} [M + Na] (m/z = 750.66), {34:1} [M + K] (m/z = 798.60), and {36:2} [M + K] (m/z = 824.56) were found to be increased in ovarian cancer. Interestingly, breast cancer-associated changes in lipid and protein profiles were also found in ovarian cancer. Thus, protein and lipid profiles accurately distinguish ovarian cancer from adjacent normal tissue samples. Common cancer-associated alterations in lipid and protein profiles were identified between ovarian and breast cancers.
authors: National Cancer Center, Goyang, Republic of Korea.
authors: Sokbom
authors: S
authors: Kang
authors: Aera
authors: A
authors: Lee
authors: Young Seung
authors: YS
authors: Park
authors: Seok Cheol
authors: SC
authors: Lee
authors: Sang Yoon
authors: SY
authors: Park
authors: Sang Yun
authors: SY
authors: Han
authors: Kwang Pyo
authors: KP
authors: Kim
authors: Young Hwan
authors: YH
authors: Kim
authors: Chong Woo
authors: CW
authors: Yoo
authors: Hark Kyun
authors: HK
authors: Kim
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077216:Carcinoma, Ovarian Epithelial; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D010713:Phosphatidylcholines / Q000032:analysis / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids; D009363:Neoplasm Proteins; D010713:Phosphatidylcholines