title: Phenanthroline-based magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
abstract: We herein report the preparation of PSMN (phenanthroline-based silica coated magnetic nanoparticles), and their applications for protein purification as selective magnetic probes of histidine-tagged proteins in cell lysates. This simple system serves as a useful alternative to existing protocols for his-tagged protein separation and as a versatile agent for transporting and anchoring proteins.
authors: Department of Wood Science and Landscape Architecture, BK21 Program, Bio-energy Research Institute, Chonnam National University, Gwangju, 500-757, S. Korea.
authors: Eun Jin
authors: EJ
authors: Cho
authors: Hyun Joo
authors: HJ
authors: Kim
authors: Younho
authors: Y
authors: Song
authors: In Seong
authors: IS
authors: Choi
authors: Hyeun-Jong
authors: HJ
authors: Bae
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D053758:Nanoparticles; D010618:Phenanthrolines / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D010618:Phenanthrolines; D011506:Proteins; D006639:Histidine