title: Identification of three novel CTL epitopes within nucleocapsid protein of Hantaan virus.
abstract: Hantaan virus (HTNV) is a member of the Hantavirus genus that causes human hemorrhagic fever with renal syndrome (HFRS) in humans. The CTL response seems to play a key role in control of viral infection, but only a few HTNV epitopes recognized by the CTLs have been reported. Herein, we screened a panel of overlapping peptides covering the HTNV nucleocapsid protein by ELISPOT assays for those that can elicit IFN-γ production in vitro. Three novel CD8(+) CTL epitopes, N197-205 (RYRTAVCGL), N245-253 (KLLPDTAAV), and N258-266 (GPATNRDYL), were defined on the nucleocapsid protein and were found to be restricted by various HLA alleles including A11, A24, and B7. The epitopes were highly conserved among the reported HTNV strains and other hantanviruses, including Dobrava-Belgrade virus and Seoul virus, supporting their potential use in vaccine designs.
authors: Center for Experimental Medicine, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou, China. mlwang_2003@yahoo.com
authors: Meiliang
authors: M
authors: Wang
authors: Yong
authors: Y
authors: Zhu
authors: Jiuping
authors: J
authors: Wang
authors: Tongde
authors: T
authors: Lv
authors: Boquan
authors: B
authors: Jin
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry* / Q000276:immunology; D002472:Cell Transformation, Viral; D017124:Conserved Sequence; D058501:Enzyme-Linked Immunospot Assay; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D006680:HLA Antigens / Q000737:chemistry / Q000276:immunology; D018063:Hantaan virus / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D006480:Hemorrhagic Fever with Renal Syndrome / Q000276:immunology / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry* / Q000276:immunology / Q000821:virology; D014758:Viral Core Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D006680:HLA Antigens; D010455:Peptides; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus; D007371:Interferon-gamma