title: White gels: an easy way to preserve methylene blue stained gels.
abstract: Methylene blue can be used as a stain for visualizing nucleic acids in polyacrylamide gel electrophoresis. However, its relatively low sensitivity and reversible binding make it a temporary stain that diffuses from the gel relatively fast. Here we describe a very simple method for fixing methylene blue bands in nucleic acid polyacrylamide gels. The procedure makes the methylene blue stain permanent and increases the visibility of the bands, also contributing to increasing the sensitivity of methylene blue.
authors: Department of Chemistry, Towson University, Towson, MD 21252, USA. asoto@towson.edu
authors: Ana Maria
authors: AM
authors: Soto
authors: David
authors: D
authors: Draper
mesh_terms: D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005782:Gels; D008751:Methylene Blue; D009696:Nucleic Acids / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents; D005782:Gels; D009696:Nucleic Acids; D008751:Methylene Blue