title: Expression pattern of CK7 and CK20 in nasal polyps, at patients with chronic rhinosinusitis with nasal polyposis.
abstract: We investigated the expression of cytokeratins 7 (CK7) and 20 (CK20) in nasal polyps on a group of 106 patients with chronic rhinosinusitis with nasal polyposis (45 women - 42% and 61 men - 58%) who benefited from surgical procedures. Harvested biological material was analyzed in the pathology laboratory through two methods: histopathological and immunohistochemical analysis. Classical histopathological method of processing the tissues initially fixed in 10% formalin was used. The tissues were then processed by paraffin impregnation, sectioned and stained with Hematoxylin-Eosin. The immunohistochemical method was based on soluble immunoenzymatic complexes - LSAB/HRP (labeled Streptavidin Biotin) method. We used DAKO LSAB 2 System HRP (Universal DAKO Labeled Streptavidin Biotin 2 System Horseradish Peroxidase). The expressions of CK7 and CK20 in nasal polyps were analyzed.
authors: Department of ENT, University of Medicine and Pharmacy of Craiova, Romania. mhlmitroi@yahoo.com
authors: Mihaela
authors: M
authors: Mitroi
authors: Alina
authors: A
authors: Căpitănescu
authors: Claudia Valentina
authors: CV
authors: Georgescu
authors: Carmen Aurelia
authors: CA
authors: Mogoantă
authors: Camelia
authors: C
authors: Popescu
authors: Monica
authors: M
authors: Georgescu
authors: G
authors: G
authors: Mitroi
authors: Elena
authors: E
authors: Ioniţă
mesh_terms: D002908:Chronic Disease; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000150:complications / Q000473:pathology; D053554:Keratin-20 / Q000378:metabolism*; D053552:Keratin-7 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications* / Q000378:metabolism / Q000473:pathology*; D012852:Sinusitis / Q000150:complications* / Q000378:metabolism / Q000473:pathology*
chemical_list: D053554:Keratin-20; D053552:Keratin-7