title: [Study on pharmacokinetics of puerarin in rats following different methods of administration of Tongqiao Sanyu prescription].
abstract: OBJECTIVE
  To study pharmacokinetic of puerarin in rats following different methods of administration of Tongqiao Sanyu prescription.
  
  
  METHODS
  Tongqiao Sanyu prescription was administered to rats by caudal vein injection, nasal administration and oral administration. Plasma samples were extracted with methanol and the plasma concentration of puerarin was analyzed by RP-HPLC. The pharmacokinetic parameters and bioavailability were calculated with Kinetica software.
  
  
  RESULTS
  The main pharmacokinetic parameters were as follows: AUC(0-infinity) of caudal vein injection was (787.99 +/- 70.44) mg x min x L(-1); AUC(0-infinity) of nasal administration was (376.56 +/- 93.93) mg x min x L(-1); AUC(0-infinity) and oral administration (The dose was decuple higher than that of caudal vein injection and nasal administration) was (491.18 +/- 110.64) mg x min x L(-1). The absolute bioavailability of puerarin was 47.78% by nasal administration and 6.23% by oral administration.
  
  
  CONCLUSIONS
  The bioavailability of nasal administration is higher than oral administration significantly, this result can provide some scientific foundantion for the method of administration and the reform of dosage form of Tongqiao Sanyu prescription.
authors: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Bejing 100102, China.
authors: Xiaolan
authors: X
authors: Chen
authors: Shouying
authors: S
authors: Du
authors: Yang
authors: Y
authors: Lu
authors: Xuejiao
authors: X
authors: Zhao
authors: Shan
authors: S
authors: Wang
authors: Pengyue
authors: P
authors: Li
authors: Xiao
authors: X
authors: Song
mesh_terms: D000818:Animals; D001682:Biological Availability; D004333:Drug Administration Routes; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D007529:Isoflavones / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D007529:Isoflavones; C033607:puerarin