title: Mass spectrometry for protein quantification in biomarker discovery.
abstract: Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion in genomic and protein bioinformatics. This leads to an increased emphasis on larger scale, faster, and more efficient methods for detecting protein biomarkers in human tissues, cells, and biofluids. Most current proteomic methodologies for biomarker discovery, however, are not highly automated and are generally labor-intensive and expensive. More automation and improved software programs capable of handling a large amount of data are essential to reduce the cost of discovery and to increase throughput. In this chapter, we discuss and describe mass spectrometry-based proteomic methods for quantitative protein analysis.
authors: Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS 4053, Indianapolis, IN 46202, USA. muwang@indiana.edu
authors: Mu
authors: M
authors: Wang
authors: Jinsam
authors: J
authors: You
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003627:Data Interpretation, Statistical; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D059748:Proteolysis; D040901:Proteomics; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D011506:Proteins