title: Interleukin-1β expression by a recombinant porcine reproductive and respiratory syndrome virus.
abstract: The cytokine interleukin-1 beta (IL-1β) is a potent inflammatory mediator in response to infection, and can be used as an immunological adjuvant. In this study, we constructed a recombinant porcine reproductive and respiratory syndrome virus (vP129/swIL1β) expressing swine IL-1β from the separate subgenomic mRNA inserted between the ORF1b and ORF2 genome region. MARC-145 cells infected with vP129/swIL1β secreted 1947 pg of IL-1β per 2 × 10(5)cells at 36 h post-infection. In vitro growth kinetics analysis in MARC-145 cells showed that the vP129/swIL1β virus had a similar replication rate as that of parental virus. We further performed in vivo characterization of the vP129/swIL1β virus in a nursery pig disease model. The vP129/swIL1β infected pigs did not show visible clinical signs, while respiratory distress and lethargy were evident in pigs infected with the parental virus. The expression of various cytokines from peripheral blood mononuclear cells measured by fluorescent microsphere immunoassay showed that IL-1β, IL-4 and IFN-γ expression levels were up-regulated in pigs infected with vP129/swIL1β at 7 and 14 days post-infection. However, no detectable level of IL-1β was found in serum samples from pigs infected with either vP129/swIL1β or parental virus. In summary, this study demonstrated a recombinant PRRSV as a useful tool to study the role of different cytokines in disease progression and immune responses, which represents a new strategy for future therapeutic application and vaccine development.
authors: Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
authors: Steven R
authors: SR
authors: Lawson
authors: Yanhua
authors: Y
authors: Li
authors: John B
authors: JB
authors: Patton
authors: Robert J
authors: RJ
authors: Langenhorst
authors: Zhi
authors: Z
authors: Sun
authors: Zhiyong
authors: Z
authors: Jiang
authors: Jane
authors: J
authors: Christopher-Hennings
authors: Eric A
authors: EA
authors: Nelson
authors: David
authors: D
authors: Knudsen
authors: Ying
authors: Y
authors: Fang
authors: Kyeong-Ok
authors: KO
authors: Chang
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D015870:Gene Expression; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000473:pathology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D019562:Viral Load; D014779:Virus Replication
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D011994:Recombinant Proteins