title: Biological response of endothelial cells to diamond-like carbon-coated NiTi alloy.
abstract: Diamond-like carbon (DLC) coatings were deposited on nearly equiatomic nickel-titanium (NiTi) alloy by arc-enhanced magnetron sputtering. The microstructure, surface morphology, chemical composition, surface free energy, protein adsorbance, and leach amount of Ni ions were assessed by Raman spectroscopy, high-resolution transmission electron microscopy (HR-TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), contact angle measurements, micro BCA™ protein assay kit, and inductively coupled plasma mass spectrometry (ICP-MS). The biological response of the endothelial cells (ECs) was evaluated by cell adhesion, morphology, viability, and expression levels of thrombogenicity-related genes. Our results show that the DLC coatings inhibit the release of Ni ions from the NiTi substrate effectively thus enhancing its biosafety. The easy adhesion, elongated morphology, and high viability of ECs on the DLC coatings suggest fast endothelialization after implantation and so application of DLC coatings improves the surface properties of NiTi in cardiovascular applications. The relationship between the surface characteristics, Ni leaching, and concomitant biological response are discussed in details.
authors: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors: Ruiqiang
authors: R
authors: Hang
authors: Ming
authors: M
authors: Zhang
authors: Shengli
authors: S
authors: Ma
authors: Paul K
authors: PK
authors: Chu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D018130:Diamond / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D007477:Ions; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D013059:Spectrum Analysis, Raman; D013816:Thermodynamics; D014025:Titanium / Q000494:pharmacology*; D019096:Vitronectin / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D007477:Ions; D019096:Vitronectin; C040654:titanium nickelide; D018130:Diamond; D009532:Nickel; D014025:Titanium