title: Effects of an oral insulin nanoparticle administration on hepatic glucose metabolism assessed by ¹³C and ²H isotopomer analysis.
abstract: The purpose of this study was to evaluate hepatic glucose metabolism of diabetic induced rats after a daily oral load of insulin nanoparticles over 2 weeks. After the 2-week treatment, an oral glucose tolerance test was performed with [U-¹³C] glucose and ²H₂O. Plasma glucose ²H and ¹³C enrichments were quantified and the contribution of glycogenolysis and gluconeogenesis to overall glucose production were estimated. Animals with the insulin nanoparticles displayed the lowest glycemia before the oral glucose tolerance test. In all animals, 75% of the total glucose production was from gluconeogenesis and glycogen synthesis was only detected in some animals. Gluconeogenic pathway was an active contributor to hepatic glucose production and the treatment with oral delivered insulin nanoparticles did not alter this contribution, suggesting that under this treatment, protocol hepatic glucose metabolism is not the most relevant target of insulin action but instead a more generalised effect in peripheral tissues.
authors: CBIOS, Faculty of Science and Health Technologies, ULHT, Lisbon, Portugal. catarinapintoreis@gmail.com
authors: Catarina Pinto
authors: CP
authors: Reis
authors: Ronald
authors: R
authors: Neufeld
authors: Francisco
authors: F
authors: Veiga
authors: Isabel V
authors: IV
authors: Figueiredo
authors: John
authors: J
authors: Jones
authors: Ana F
authors: AF
authors: Soares
authors: Patrícia
authors: P
authors: Nunes
authors: Christiane
authors: C
authors: Damgé
authors: Rui A
authors: RA
authors: Carvalho
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002247:Carbon Isotopes; D003903:Deuterium; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002247:Carbon Isotopes; D007328:Insulin; D003903:Deuterium; D005947:Glucose