title: Zernike phase contrast cryo-electron tomography of whole mounted frozen cells.
abstract: Cryo-electron tomography of frozen hydrated cells has provided cell biologists with an indispensable tool for delineating three-dimensional arrangements of cellular ultrastructure. To avoid the damage induced by electron irradiation, images of frozen hydrated biological specimens are generally acquired under low-dose conditions, resulting in weakly contrasted images that are difficult to interpret, and in which ultrastructural details remain ambiguous. Zernike phase contrast transmission electron microscopy can improve contrast, and can also fix a fatal problem related to the inherent low contrast of conventional electron microscopy, namely, image modulation due to the unavoidable setting of deep defocus. In this study, we applied cryo-electron tomography enhanced with a Zernike phase plate, which avoids image modulation by allowing in-focus setting. The Zernike phase contrast cryo-electron tomography has a potential to suppress grainy background generation. Due to the smoother background in comparison with defocus phase contrast cryo-electron tomography, Zernike phase contrast cryo-electron tomography could yield higher visibility for particulate or filamentous ultrastructure inside the cells, and allowed us to clearly recognize membrane protein structures.
authors: Division of Nano-Structure Physiology, Okazaki Institute for Integrative Bioscience, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan.
authors: Yoshiyuki
authors: Y
authors: Fukuda
authors: Kuniaki
authors: K
authors: Nagayama
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy / Q000379:methods; D004173:Dipodomys; D055032:Electron Microscope Tomography / Q000379:methods; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast / Q000379:methods; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D008565:Membrane Proteins