title: Bactericidal effects of antimicrobial agents on epithelial cell-associated Pseudomonas aeruginosa.
abstract: It is not clear whether antipseudomonal agents can kill cell-associated bacteria within a short time. Madin-Darby canine kidney (MDCK) and A549 cells were infected with Pseudomonas aeruginosa ATCC 27853 and PAO1 and the bactericidal activity of ceftazidime, imipenem, meropenem, gentamicin, and ciprofloxacin against the organisms was investigated. In both MDCK and A549 cells, β-lactams could not kill epithelial cell-associated bacteria within 2 h. Gentamicin at concentrations ≤32 μg/ml killed more than 99% of epithelial cell-associated bacteria. Ciprofloxacin at 0.5 μg/ml killed more than 99.9% of MDCK cell-associated bacteria. Ciprofloxacin has the strongest and most rapid bactericidal activity against epithelial cell-associated bacteria, which may be explained by the combination of potent in-vitro bactericidal activity and high penetration ability into epithelial cells.
authors: Department of Clinical Microbiology with Epidemiological Research & Management and Analysis of Infectious Diseases (C-MERMAID), Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8574, Japan. hiraichi@med.tohoku.ac.jp
authors: Yoichi
authors: Y
authors: Hirakata
authors: Hisakazu
authors: H
authors: Yano
authors: Kazuaki
authors: K
authors: Arai
authors: Miho
authors: M
authors: Kitagawa
authors: Masumitsu
authors: M
authors: Hatta
authors: Hiroyuki
authors: H
authors: Kunishima
authors: Mitsuo
authors: M
authors: Kaku
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002939:Ciprofloxacin / Q000494:pharmacology; D004285:Dogs; D004847:Epithelial Cells / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D061985:Madin Darby Canine Kidney Cells; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002939:Ciprofloxacin; D015378:Imipenem