title: Analysis of phenolic compounds by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry in senescent and water-stressed tobacco.
abstract: Evaluation of a significant part of the phenylpropanoid pathway metabolites is facilitated by the fast high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC-MS/MS) analytical method. The technology described was applied in tobacco plants (Nicotiana tabacum L. cv. Wisconsin) to identify 20 phenolic compounds and to detect differences in phenylpropanoid profiles in two types of experiments. In the first one, senescent and non-senescent parts of flowering plants were compared, while in the second, watered plants were compared with water-stressed young plants. The 20 identified phenolic compounds were: seven hydroxycinnamoylquinic acids, seven hydroxycinnamic acid glucosides, one salicylic acid glucoside, two conjugated flavonols with disaccharides, and three hydroxycinnamic acid amides (HCAA) of putrescine. In general, the levels of phenylpropanoid compounds increased under water stress or senescent conditions, with the exception of HCAA, which decreased in senescent samples, and 4-O-p-coumaroylquinic acid and trihydroxycinamic acid-O-glucoside, which did not change in both experiments. The main product in all the samples was 5-O-caffeoylquinic acid (neochlorogenic acid). Another compound, kaempferol-7-O-neohesperidoside, was tentatively identified for the first time in tobacco plants. This method, which can be applied in other plant species, allows a simple and efficient comparative study of metabolite profile variations (qualitative and quantitative) in response to different physiological and/or environmental plant situations.
authors: Departament de Productes Naturals, Biologia Vegetal i Edafologia, Facultat de Farmàcia, Universitat de Barcelona, Avinguda Joan XXIII s/n, Avda. Diagonal, 643, 08028 Barcelona, Catalonia, Spain.
authors: Laura
authors: L
authors: Torras-Claveria
authors: Olga
authors: O
authors: Jáuregui
authors: Carles
authors: C
authors: Codina
authors: Antonio F
authors: AF
authors: Tiburcio
authors: Jaume
authors: J
authors: Bastida
authors: Francesc
authors: F
authors: Viladomat
mesh_terms: D000222:Adaptation, Physiological; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003681:Dehydration / Q000378:metabolism*; D035264:Flowers / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D020005:Propanols / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013312:Stress, Physiological; D053719:Tandem Mass Spectrometry / Q000379:methods; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism*; D014867:Water
chemical_list: D010636:Phenols; D010937:Plant Growth Regulators; D020005:Propanols; D014867:Water; C439395:1-phenylpropanol