title: Distinctive amino acid composition profiles in salivary proteins of the tick Ixodes scapularis.
abstract: Bioinformatic analysis of the amino acid composition of proteins of the tick Ixodes scapularis showed that, in comparison to other secreted proteins, salivary proteins in general have higher frequencies of polar residues and lower frequencies of the non-polar residues leucine and valine. Computer prediction of linear B-cell epitopes showed that polar residues were associated with the presence of high-quality epitopes and that tick salivary proteins included significantly more proteins with predicted high-quality epitopes than did other secreted proteins. The results provided no evidence that salivary proteins as a whole have evolved characteristics minimizing their antigenicity to the vertebrate host. Certain salivary proteins may indeed have evolved low antigenicity, but the I. scapularis sialome include at least some apparently antigenic proteins that might be tested experimentally to determine whether they would be suitable candidates for anti-tick vaccines.
authors: Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA. austin@biol.sc.edu
authors: Austin L
authors: AL
authors: Hughes
authors: Robert
authors: R
authors: Friedman
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D019295:Computational Biology / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000235:genetics; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D018884:Ixodes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology
chemical_list: D000941:Antigens; D018985:Epitopes, B-Lymphocyte; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides