title: The effect of topical negative pressure on wound biofilms using an in vitro wound model.
abstract: Chronic non-healing wounds affect a significant number of patients worldwide. Although the etiologies of these wounds are varied, bacterial infection has been suggested as a major factor responsible for the perpetual inflammation and tissue destruction observed in such wounds. Recent evidence has emerged suggesting that bacterial biofilms in particular may have a significant role in this process. At the same time, topical negative pressure dressing is gaining acceptance as a therapy which promotes healing in recalcitrant wounds. In this study an in vitro Pseudomonas aeruginosa biofilm model was developed to mimic potential surface wound biofilms. Topical negative pressure dressing was applied to the model and the effects of topical negative pressure dressing on the in vitro wound biofilms were examined using both quantitative microbiological counting technique and imaging studies. The results demonstrated a small but statistically significant reduction in biofilm bacteria at 2 weeks when exposed to topical negative pressure. When this was combined with silver impregnated foam, the reduction was far more significant and was observable within 24 hours. Microscopically, it was also noted that topical negative pressure compressed the biofilm architecture with a reduction in thickness and diffusion distance.
authors: Department of Maxillofacial and Plastic Surgery, South Western Sydney Medical School, University of Sydney, Sydney, Australia.
authors: Quan D
authors: QD
authors: Ngo
authors: Karen
authors: K
authors: Vickery
authors: Anand K
authors: AK
authors: Deva
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D006801:Humans; D050296:Microbial Viability; D008954:Models, Biological; D054843:Negative-Pressure Wound Therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D018030:Silver Compounds / Q000494:pharmacology*; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects; D014946:Wound Infection / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D018030:Silver Compounds