title: Detection of BRAF mutations in patients with hairy cell leukemia and related lymphoproliferative disorders.
abstract: Hairy cell leukemia has been shown to be strongly associated with the BRAF V600E mutation. We screened 59 unenriched archived bone marrow aspirate and peripheral blood samples from 51 patients with hairy cell leukemia using high resolution melting analysis and confirmatory Sanger sequencing. The BRAF V600E mutation was detected in 38 samples (from 36 patients). The BRAF V600E mutation was detected in all samples with disease involvement above the limit of sensitivity of the techniques used. Thirty-three of 34 samples from other hematologic malignancies were negative for BRAF mutations. A BRAF K601E mutation was detected in a patient with splenic marginal zone lymphoma. Our data support the recent finding of a disease defining point mutation in hairy cell leukemia. Furthermore, high resolution melting with confirmatory Sanger sequencing are useful methods that can be employed in routine diagnostic laboratories to detect BRAF mutations in patients with hairy cell leukemia and related lymphoproliferative disorders.
authors: Peter MacCallum Cancer Centre, Division of Cancer Medicine, East Melbourne, Victoria, Australia.
authors: Piers A
authors: PA
authors: Blombery
authors: Stephen Q
authors: SQ
authors: Wong
authors: Chelsee A
authors: CA
authors: Hewitt
authors: Alexander
authors: A
authors: Dobrovic
authors: Ellen L
authors: EL
authors: Maxwell
authors: Surender
authors: S
authors: Juneja
authors: George
authors: G
authors: Grigoriadis
authors: David A
authors: DA
authors: Westerman
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis / Q000235:genetics*; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf