title: Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces.
abstract: Antibiotic production and cell differentiation in Streptomyces is stimulated by micromolar levels of Cu(2+) . Here, we knocked out the Sco1/SenC family copper chaperone (ScoC) encoded in the conserved gene cluster 'sco' (the S treptomycescopper utilization) in Streptomyces coelicolor A3(2) and S. griseus. It is known that the Sco1/SenC family incorporates Cu(2+)  into the active centre of cytochrome oxidase (cox). The knockout caused a marked delay in antibiotic production and aerial mycelium formation on solid medium, temporal pH decline in glucose-containing liquid medium, and significant reduction of cox activity in S. coelicolor. The scoC mutant produced two- to threefold higher cellular mass of the wild type exhibiting a marked cox activity in liquid medium supplied with 10 µM CuSO(4) , suggesting that ScoC is involved in not only the construction but also the deactivation of cox. The scoC mutant was defective in the monoamine oxidase activity responsible for cell aggregation and sedimentation. These features were similarly observed with regard to the scoC mutant of S. griseus. The scoC mutant of S. griseus was also defective in the extracellular activity oxidizing N,N'-dimethyl-p-phenylenediamine sulfate. Addition of 10 µM CuSO(4)  repressed the activity of the conserved promoter preceding scoA and caused phenylalanine auxotrophy in some Streptomyces spp. probably because of the repression of pheA; pheA encodes prephenate dehydratase, which is located at the 3' terminus of the putative operon structure. Overall, the evidence indicates that Sco is crucial for the utilization of copper under a low-copper condition and for the activation of the multiple Cu(2+) -containing oxidases that play divergent roles in the complex physiology of Streptomyces.
authors: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Japan.
authors: Masahiro
authors: M
authors: Fujimoto
authors: Akio
authors: A
authors: Yamada
authors: Junpei
authors: J
authors: Kurosawa
authors: Akihiro
authors: A
authors: Kawata
authors: Teruhiko
authors: T
authors: Beppu
authors: Hideaki
authors: H
authors: Takano
authors: Kenji
authors: K
authors: Ueda
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D018533:Biomass; D003300:Copper / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D055786:Gene Knockout Techniques; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D013305:Streptomyces griseus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D018832:Molecular Chaperones; D003300:Copper; D010088:Oxidoreductases; D005947:Glucose