title: Identification of a p-cresol degradation pathway by a GFP-based transposon in Pseudomonas and its dominant expression in colonies.
abstract: In this study, the chromosome-encoded pcuRCAXB genes that are required for p-cresol degradation have been identified by using a newly constructed green fluorescent protein (GFP)-based promoter probe transposon in the long-chain alkylphenol degrader Pseudomonas alkylphenolia. The deduced amino acid sequences of the genes showed the highest identities at the levels of 65-93% compared with those in the databases. The transposon was identified to be inserted in the pcuA gene, with the promoterless gfp gene being under the control of the pcu catabolic gene promoter. The expression of GFP was positively induced by p-cresol and was about 10 times higher by cells grown on agar than those in liquid culture. In addition, phydroxybenzoic acid was detected during p-cresol degradation. These results indicate that P. alkylphenolia additionally possesses a protocatechuate ortho-cleavage route for pcresol degradation that is dominantly expressed in colonies.
authors: Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea.
authors: Ah Ra
authors: AR
authors: Cho
authors: Eun Jin
authors: EJ
authors: Lim
authors: Yaligara
authors: Y
authors: Veeranagouda
authors: Kyoung
authors: K
authors: Lee
mesh_terms: D019677:Artificial Gene Fusion; D003408:Cresols / Q000378:metabolism*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010226:Parabens / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D003408:Cresols; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010226:Parabens; D049452:Green Fluorescent Proteins; C032538:4-cresol; C038193:4-hydroxybenzoic acid