title: [Simultaneous determination of 9 heterocyclic aromatic amine in poultry products by solid-phase extraction-high performance liquid chromatography].
abstract: A method was developed for the simultaneous determination of 9 heterocyclic aromatic amines (HAAs) including 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,4-dimethylimidazo[4, 5-f] quinoline (MeIQ), 2-amino-3, 8-dimethylimidazo [4, 5-f] quinoxaline (MeIQx), 2-amino-3, 4, 8-trimethyl-imidazo [4,5-f quinoxaline (4,8-DiMeIQx), 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP), 3-amino-1 -methyl-5H-pyrido [4, 3-b] indole (Trp-p-2), 3-amino-1, 4-dimethyl-5H-pyrido [4,3-b] indole (Trp-p-1), 9H-pyrido [3,4-b] indole (Norharman), 1-methyl-9H-pyrido 3, 4-b] indole (Harman)) in poultry products using solid-phase extraction-high performance liquid chromatography (SPE-HPLC). The performance of 3 different organic extraction solutions, 2 solid phase extraction (SPE) procedures, three different reversed-phase columns and five different mobile phases were tested for optimizing separation conditions of the 9 HAAs from poultry products. In the end, ethyl acetate was selected as the extraction solution, and the extract was purified with propylsulfonic acid silica (PRS) and C18 SPE columns. The analysis was performed on a TSK-gel ODS-80TM column using a gradient elution with the mobile phases of 0.05 mol/L aqueous acetic acid-ammonium acetate buffer (pH 3.4) and acetonitrile. The results showed that the average recoveries (n = 6) of the 9 HAAs spiked in meat samples at 3 levels ranged from 60.47% to 90.55% with the relative standard deviations (RSDs) between 0.49% and 9.74%, and the limits of detection (LODs, S/N = 3) were in the range of 0.1 - 3.6 microg/kg. The method is simple, rapid, accurate and sensitive enough for the analysis of HAAs in poultry products.
authors: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors: Bin
authors: B
authors: Shao
authors: Zengqi
authors: Z
authors: Peng
authors: Hongsheng
authors: H
authors: Yang
authors: Guanghong
authors: G
authors: Wu
authors: Yao
authors: Y
authors: Yao
authors: Kehui
authors: K
authors: Wan
mesh_terms: D000588:Amines / Q000032:analysis*; D000818:Animals; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D006571:Heterocyclic Compounds / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis; D011202:Poultry Products / Q000032:analysis*; D011810:Quinoxalines / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D000588:Amines; D002273:Carcinogens; D006571:Heterocyclic Compounds; D007093:Imidazoles; D011810:Quinoxalines; C036990:2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; C045421:3,4,8-trimethylimidazo(4,5-f)quinoxalin-2-amine