title: Enhancement of MSC adhesion and therapeutic efficiency in ischemic heart using lentivirus delivery with periostin.
abstract: Many approaches have shown beneficial effects of modified mesenchymal stem cells (MSCs) for treatment of infarcted myocardium, but have primarily focused on enhancing the survival of transplanted MSCs. Here, we show the dual benefits of periostin-overexpressing MSCs (p-MSCs) for infarcted myocardium. P-MSCs led to the marked histological and functional recovery of infarcted myocardium by enhancing survival of MSCs and directly preventing apoptosis of cardiomyocytes. Survival of p-MSCs themselves and cardiomyocytes co-cultured with p-MSCs or treated with the conditioned media from p-MSCs was significantly increased under hypoxic conditions. Decreases in adhesion-related integrins were reversed in cardiomyocytes co-cultured with p-MSCs, followed by increases in p-PI3K and Akt, indicating that periostin activates the PI3K pathway through adhesion-related integrins. When p-MSCs were injected into myocardial infarcted rats, histological pathology and cardiac function were significantly improved compared to MSC-injected controls. Thus, periostin might be a new target of therapeutic treatments using MSCs as carriers for infarcted myocardium.
authors: Cardiology Division, Myongji Hospital, Kwandong University College of Medicine, Goyangsi, Gyeonggido, Republic of Korea.
authors: Yun-Hyeong
authors: YH
authors: Cho
authors: Min-Ji
authors: MJ
authors: Cha
authors: Byeong-Wook
authors: BW
authors: Song
authors: Il-Kwon
authors: IK
authors: Kim
authors: Heesang
authors: H
authors: Song
authors: Woochul
authors: W
authors: Chang
authors: Soyeon
authors: S
authors: Lim
authors: Onju
authors: O
authors: Ham
authors: Se-Yeon
authors: SY
authors: Lee
authors: Eunmi
authors: E
authors: Choi
authors: Hyuck Moon
authors: HM
authors: Kwon
authors: Ki-Chul
authors: KC
authors: Hwang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D018014:Gene Transfer Techniques; D016086:Lentivirus / Q000235:genetics*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017202:Myocardial Ischemia / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015815:Cell Adhesion Molecules; D017077:Culture Media, Conditioned