title: Magnesium used in bioabsorbable stents controls smooth muscle cell proliferation and stimulates endothelial cells in vitro.
abstract: Magnesium-based bioabsorbable cardiovascular stents have been developed to overcome limitations of permanent metallic stents, such as late stent thrombosis. During stent degradation, endothelial and smooth muscle cells will be exposed to locally high magnesium concentrations with yet unknown physiological consequences. Here, we investigated the effects of elevated magnesium concentrations on human coronary artery endothelial and smooth muscle cell (HCAEC, HCASMC) growth and gene expression. In the course of 24 h after incubation with magnesium chloride solutions (1 or 10 mM) intracellular magnesium level in HCASMC raised from 0.55 ± 0.25 mM (1 mM) to 1.38 ± 0.95 mM (10 mM), while no increase was detected in HCAEC. Accordingly, a DNA microarray-based study identified 69 magnesium regulated transcripts in HCAEC, but 2172 magnesium regulated transcripts in HCASMC. Notably, a significant regulation of various growth factors and extracellular matrix components was observed. In contrast, viability and proliferation of HCAEC were increased at concentrations of up to 25 mM magnesium chloride, while in HCASMC viability and proliferation appeared to be unaffected. Taken together, our data indicate that magnesium halts smooth muscle cell proliferation and stimulates endothelial cell proliferation, which might translate into a beneficial effect in the setting of stent associated vascular injury.
authors: University of Rostock, Medical Faculty, Institute for Biomedical Engineering, Rostock, Germany. katrin.sternberg@uni-rostock.de
authors: Katrin
authors: K
authors: Sternberg
authors: Matthias
authors: M
authors: Gratz
authors: Kathleen
authors: K
authors: Koeck
authors: Joerg
authors: J
authors: Mostertz
authors: Robert
authors: R
authors: Begunk
authors: Marian
authors: M
authors: Loebler
authors: Beatrice
authors: B
authors: Semmling
authors: Anne
authors: A
authors: Seidlitz
authors: Petra
authors: P
authors: Hildebrandt
authors: Georg
authors: G
authors: Homuth
authors: Niels
authors: N
authors: Grabow
authors: Conny
authors: C
authors: Tuemmler
authors: Werner
authors: W
authors: Weitschies
authors: Klaus-Peter
authors: KP
authors: Schmitz
authors: Heyo K
authors: HK
authors: Kroemer
mesh_terms: D020341:Absorbable Implants; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008274:Magnesium; D008422:Materials Testing / Q000379:methods; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D015607:Stents
chemical_list: D008274:Magnesium