title: A two-stage enzymatic process for synthesis of extremely pure high oleic glycerol monooleate.
abstract: This paper presents a research interest concentrating on aims to establish a feasible industrial process for enzymatic production of highly pure glycerol monooleate (GMO). The synthesis of high oleic glycerol monooleate by enzymatic glycerolysis of high oleic sunflower oil, using Novozyme 435 as the biocatalyst, in a binary solvent mixture of tert-butanol and tert-pentanol (80/20, v/v), at a lab scale has been studied. A yield of 75.31% monoacylglycerol has been achieved at the first stage. A yield of 93.3% GMO was finally reached after further purification at the second stage. To evaluate the possibility of the process for industrialization, production of GMO was performed at a pilot-plant scale under the correspondingly adjusted conditions. A yield of 68.17% and 93.4% of GMO was obtained, respectively, at the end of the three stages.
authors: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, People's Republic of China.
authors: Qisi
authors: Q
authors: Zhu
authors: Tie
authors: T
authors: Li
authors: Yonghua
authors: Y
authors: Wang
authors: Bo
authors: B
authors: Yang
authors: Yongjun
authors: Y
authors: Ma
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D003470:Culture Media; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides / Q000096:biosynthesis* / Q000302:isolation & purification*; D008049:Lipase / Q000378:metabolism*; D000439:Pentanols; D010938:Plant Oils / Q000378:metabolism*; D012997:Solvents; D000074242:Sunflower Oil; D020002:tert-Butyl Alcohol
chemical_list: D003470:Culture Media; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides; D000439:Pentanols; D010938:Plant Oils; D012997:Solvents; D000074242:Sunflower Oil; C005953:monoolein; C093667:Novozyme 435; D008049:Lipase; D020002:tert-Butyl Alcohol