title: The biosynthesis of the polyether antibiotic nanchangmycin is controlled by two pathway-specific transcriptional activators.
abstract: The nanchangmycin (NAN) produced by Streptomyces nanchangensis NS3226 is a polyether antibiotic resembling monensin in their gene clusters and the chemical structures. They can inhibit gram-positive bacteria and be a growth promoter for ruminants. Within the nanchangmycin gene cluster (nan), we identified that two SARP-family regulatory genes, nanR1 and nanR2, were both required to activate the transcription of all nan polyketide genes. Overexpression of NanR1 and NanR2 in wild-type increase NAN yields by at least three folds. Bioinformatic analysis of the immediate upstream DNA sequence of each nan gene and quantitative real-time RT-PCR analysis of the nan operons identified five putative SARP binding sites. Moreover, deletion of an AraC-family repressor gene nanR4 increased expression of NanR1 and R2 and led to a threefold increase in NAN production.
authors: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
authors: Qing
authors: Q
authors: Yu
authors: Aiqin
authors: A
authors: Du
authors: Tiangang
authors: T
authors: Liu
authors: Zixin
authors: Z
authors: Deng
authors: Xinyi
authors: X
authors: He
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D001665:Binding Sites; D004987:Ethers / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013141:Spiro Compounds / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D004987:Ethers; D013141:Spiro Compounds; C452419:nanchangmycin