title: Point-spread function engineering to reduce the impact of spherical aberration on 3D computational fluorescence microscopy imaging.
abstract: Wavefront encoding (WFE) with different cubic phase mask designs was investigated in engineering 3D point-spread functions (PSF) to reduce their sensitivity to depth-induced spherical aberration (SA) which affects computational complexity in 3D microscopy imaging. The sensitivity of WFE-PSFs to defocus and to SA was evaluated as a function of phase mask parameters using mean-square-error metrics to facilitate the selection of mask designs for extended-depth-of-field (EDOF) microscopy and for computational optical sectioning microscopy (COSM). Further studies on pupil phase contribution and simulated WFE-microscope images evaluated the engineered PSFs and demonstrated SA insensitivity over sample depths of 30 μm. Despite its low sensitivity to SA, the successful WFE design for COSM maintains a high sensitivity to defocus as it is desired for optical sectioning.
authors: Department of Electrical and Computer Engineering, the University of Memphis, Memphis, TN 38152, USA. cpreza@memphis.edu
authors: Shuai
authors: S
authors: Yuan
authors: Chrysanthe
authors: C
authors: Preza