title: A liquid phase based C. elegans behavioral analysis system identifies motor activity loss in a nematode Parkinson's disease model.
abstract: Motor activity of Caenorhabditis elegans is widely used to study the mechanisms ranging from basic neuronal functions to human neurodegenerative diseases. It may also serve as a paradigm to screen for potential therapeutic reagents treating these diseases. Here, we developed an automated, 96-well plate and liquid phase based system that quantifies nematode motor activity in real time. Using this system, we identified an adult-onset, ageing-associated motor activity loss in a transgenic nematode line expressing human pathogenic G2019S mutant LRRK2 (leucine-rich repeat kinase 2), the leading genetic cause of Parkinson's disease characterized by dopaminergic neurodegeneration associated motor deficient mainly in elder citizens. Thus, our system may be used as a platform to screen for potential therapeutic drugs treating Parkinson's disease. It can also be used to monitor motor activity of nematodes in liquid phase at similar scenario.
authors: Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, United States.
authors: Maohua
authors: M
authors: Zheng
authors: Olga
authors: O
authors: Gorelenkova
authors: Jiong
authors: J
authors: Yang
authors: Zhaoyang
authors: Z
authors: Feng
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000978:Antiparkinson Agents / Q000627:therapeutic use; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D001330:Electronic Data Processing / Q000295:instrumentation / Q000379:methods; D018698:Glutamic Acid / Q000235:genetics; D006801:Humans; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D007980:Levodopa / Q000627:therapeutic use; D009043:Motor Activity / Q000235:genetics / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D010300:Parkinson Disease / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012694:Serine / Q000235:genetics; D012984:Software
chemical_list: D000978:Antiparkinson Agents; D029742:Caenorhabditis elegans Proteins; D018698:Glutamic Acid; D012694:Serine; D007980:Levodopa; C495280:LRRK2 protein, human; D000071158:Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; D017346:Protein Serine-Threonine Kinases