title: Solution NMR structure of BT_0084, a conjugative transposon lipoprotein from Bacteroides thetaiotamicron.
abstract: Here we describe the solution NMR structure of the 120 amino acid fragment of BT_0084, without the N-terminal lipoprotein targeting sequence, encoded in a conjugative transposon (CTn) in the genome of Bacteroides thetaiotamicron. BT_0084 belongs to a conserved family of TraQ lipoproteins that are encoded at the end of the tra operon, which contains genes essential for transfer of CTns. The structure belongs to the immunoglobulin superfamily and shares structural similarity, albeit low sequence identity (< 15%), to other proteins involved in pili production for bacterial cell attachment. Although its role in repression of CTn transfer remains to be determined, the structure of BT_0084 reported here represents the first from the Bacteroides TraQ family and should facilitate further understanding of the tra operon-regulated transfer of CTns.
authors: Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056 and the Northeast Structural Genomics Consortium. ramelota@muohio.edu.
authors: Theresa A
authors: TA
authors: Ramelot
authors: Yunhuang
authors: Y
authors: Yang
authors: Rong
authors: R
authors: Xiao
authors: Thomas B
authors: TB
authors: Acton
authors: John K
authors: JK
authors: Everett
authors: Gaetano T
authors: GT
authors: Montelione
authors: Michael A
authors: MA
authors: Kennedy
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001439:Bacteroides / Q000737:chemistry* / Q000235:genetics; D004251:DNA Transposable Elements; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008074:Lipoproteins