title: Dental afferents project onto gustatory neurons in the nucleus of the solitary tract.
abstract: The aim of this study was to investigate the inferior alveolar nerve (IAN) and chorda tympani (CT) projections onto gustatory neurons of the nucleus of the solitary tract (NST) in the rat by immunochemical and electrophysiological techniques. IAN afferents were retrogradely labeled. NST neurons were labeled either by retrograde tracer injection into the parabrachial nucleus (PBN) or by c-Fos mapping after CT activation. NST neurons responding to tastant stimulation were recorded in vivo before and after electrical stimulation of the IAN. Results from the immunolabeling approach showed IAN boutons "en passant" apposed to retrogradely labeled neurons from PBN and to CT-activated neurons in the NST. Recordings of single NST neurons showed that the electrical stimulation of the IAN significantly decreased CT gustatory responses. Analysis of these data provides an anatomical and physiological basis to support trigeminal dental and gustatory interactions within the brainstem.
authors: UFR Odontologie, Université Paris Denis Diderot, 5, rue Garancière, 75006 Paris, France.
authors: A
authors: A
authors: Braud
authors: A
authors: A
authors: Vandenbeuch
authors: F
authors: F
authors: Zerari-Mailly
authors: Y
authors: Y
authors: Boucher
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000033:anatomy & histology / Q000502:physiology; D002814:Chorda Tympani Nerve / Q000033:anatomy & histology* / Q000502:physiology; D003712:Dendrites / Q000502:physiology / Q000648:ultrastructure; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000502:physiology; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008340:Mandibular Nerve / Q000033:anatomy & histology* / Q000502:physiology; D009334:Neck Muscles / Q000294:innervation; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats; D017207:Rats, Sprague-Dawley; D012154:Reticular Formation / Q000033:anatomy & histology / Q000502:physiology; D011984:Sensory Receptor Cells / Q000166:cytology* / Q000502:physiology; D059010:Single-Cell Analysis; D017552:Solitary Nucleus / Q000033:anatomy & histology* / Q000502:physiology; D013649:Taste / Q000502:physiology*; D014059:Tongue / Q000294:innervation
chemical_list: D005456:Fluorescent Dyes; D016760:Proto-Oncogene Proteins c-fos