title: Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation.
abstract: Bone cells of the osteoblastic lineage are responsive to the local mechanical environment. Through integration of a number of possible loading-induced regulatory stimuli, osteocyte, osteoblast, and osteoclast behaviour is organized to fashion a skeletal element of sufficient strength and toughness to resist fracture and crack propagation. Early pre-osteogenic responses had been determined in vivo and this led to the development of bone organ culture models to elucidate other pre-osteogenic responses where osteocytes and osteoblasts retain the natural orientation, connections and attachments to their native extracellular matrix. The application of physiological mechanical loads to bone in these organ culture models generates the regulatory stimuli. As a consequence, these experiments can be used to illustrate the distinctive mechanisms by which osteocytes and osteoblasts respond to mechanical loads and also differences in these responses, suggesting co-ordinated and cooperatively between cell populations. Organ explant cultures are awkward to maintain, and have a limited life, but length of culture times are improving. Monolayer cultures are much easier to maintain and permit the application of a particular mechanical stimulation to be studied in isolation; mainly direct mechanical strain or fluid shear strains. These allow for the response of a single cell type to the applied mechanical stimulation to be monitored precisely.The techniques that can be used to apply mechanical strain to bone and bone cells have not advanced greatly since the first edition. The output from such experiments has, however, increased substantially and their importance is now more broadly accepted. This suggests a growing use of these approaches and an increasing awareness of the importance of the mechanical environment in controlling normal bone cell behaviour. We expand the text to include additions and modifications made to the straining apparatus and update the research cited to support this growing role of cell and organ culture models to analyze responses of bone cells to mechanical stimulation.
authors: Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College Street, London, UK. apitsill@rvc.ac.uk
authors: Andrew A
authors: AA
authors: Pitsillides
authors: Simon C F
authors: SC
authors: Rawlinson
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D002645:Chickens; D004285:Dogs; D004867:Equipment Design; D057446:Hydrodynamics; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology* / Q000378:metabolism; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D051381:Rats; D013314:Stress, Mechanical