title: Development of microsatellite markers for Ophiocordyceps sinensis (Ophiocordycipitaceae) using an ISSR-TAIL-PCR method.
abstract: OBJECTIVE
  Microsatellite primers were developed for Ophiocordyceps sinensis, an endangered medicinal fungus endemic to the Tibetan Plateau, to investigate its genetic diversity and population structure.
  
  
  RESULTS
  An inter-simple sequence repeat (ISSR)-thermal asymmetric interlaced (TAIL)-PCR method was established to develop microsatellite markers. A total of 30 perfect and imperfect microsatellites were identified in 48 individuals of O. sinensis from five provinces within China representing different populations. Seventeen loci were polymorphic with two to four alleles per locus, while 13 were monomorphic.
  
  
  CONCLUSIONS
  The results indicate that the microsatellite markers developed here may be used in studies of population genetics and conservation biology of O. sinensis. Furthermore, the ISSR-TAIL-PCR method is a simple strategy for microsatellite marker development.
authors: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors: Xiao-Liang
authors: XL
authors: Wang
authors: Rui-Heng
authors: RH
authors: Yang
authors: Yi-Jian
authors: YJ
authors: Yao
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D006999:Hypocreales / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D005819:Genetic Markers