title: The human C-type lectin-like receptor CLEC-1 is upregulated by TGF-β and primarily localized in the endoplasmic membrane compartment.
abstract: The orphan receptor CLEC-1 is part of a subfamily of C-type lectin-like receptors, which is encoded in the human natural killer gene complex and comprises several pattern recognition receptors important for innate immune functions. As information on human CLEC-1 is still very limited, we aimed to further characterize this receptor. Similar to another subfamily member, LOX-1, expression of CLEC-1 mRNA was detected in myeloid cells as well as in endothelial cells. CLEC-1 protein displayed N-linked glycosylation and formed dimers. However, in contrast to other members of the subfamily, expression levels were upregulated by transforming growth factor (TGF)-β, but not significantly affected by proinflammatory stimuli. It is intriguing that human CLEC-1 could only be detected intracellularly with a staining pattern resembling endoplasmic reticulum proteins. Neither TGF-β nor inflammatory stimuli could promote significant translocation to the cell surface. These findings are in accordance with a primarily intracellular localization and function of human CLEC-1.
authors: Department of Vascular Biology and Thrombosis Research Immunology Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, Austria. s.sattler@imperial.ac.uk
authors: S
authors: S
authors: Sattler
authors: D
authors: D
authors: Reiche
authors: C
authors: C
authors: Sturtzel
authors: I
authors: I
authors: Karas
authors: S
authors: S
authors: Richter
authors: M L
authors: ML
authors: Kalb
authors: W
authors: W
authors: Gregor
authors: E
authors: E
authors: Hofer
mesh_terms: D001483:Base Sequence; D003713:Dendritic Cells / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D037181:Lectins, C-Type; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta