title: Quantifying the reversible association of thermosensitive nanoparticles.
abstract: Under many conditions, biomolecules and nanoparticles associate by means of attractive bonds, due to hydrophobic attraction. Extracting the microscopic association or dissociation rates from experimental data is complicated by the dissociation events and by the sensitivity of the binding force to temperature (T). Here we introduce a theoretical model that combined with light-scattering experiments allows us to quantify these rates and the reversible binding energy as a function of T. We apply this method to the reversible aggregation of thermoresponsive polystyrene/poly(N-isopropylacrylamide) core-shell nanoparticles, as a model system for biomolecules. We find that the binding energy changes sharply with T, and relate this remarkable switchable behavior to the hydrophobic-hydrophilic transition of the thermosensitive nanoparticles.
authors: Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
authors: Alessio
authors: A
authors: Zaccone
authors: Jerome J
authors: JJ
authors: Crassous
authors: Benjamin
authors: B
authors: Béri
authors: Matthias
authors: M
authors: Ballauff
mesh_terms: D057446:Hydrodynamics; D007700:Kinetics; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics