title: Spectrophotometric determination of bacitracin in bulk drug as dabsyl derivative in a range of visible light.
abstract: A fast spectrophotometric method has been developed for bacitracin identification and determination after condensation reaction with dabsyl chloride. In addition, determination of dye stability of sulfonamide derivative and identification of the molar ratio of reagents was done at various time-points. The developed method has a good linearity with very broad spectrum, correlation coefficient of r = 0.9972, good precision (RSD = 1.54 +/- 0.11%), and recovery at three different levels of concentration was found between 98.33% and 103.47%. Usefulness of the method was demonstrated by positive results obtained during determination of bacitracin concentration in bulk drug.
authors: Department of Inorganic and Analytical Chemistry, Jagiellonian University, Medical College, 9 Medyczna St., 30-688 Kraków, Poland. jankrzek@cm-uj.krakow.pl
authors: Jan
authors: J
authors: Krzek
authors: Joanna
authors: J
authors: Piotrowska
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001414:Bacitracin / Q000032:analysis*; D002138:Calibration; D003116:Color; D004355:Drug Stability; D012015:Reference Standards; D013053:Spectrophotometry / Q000592:standards; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000592:standards; D013997:Time Factors; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D001414:Bacitracin; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D004124:p-Dimethylaminoazobenzene