title: Gentiana asclepiadea protects human cells against oxidation DNA lesions.
abstract: The objectives of this study were to examine whether the methanolic and aqueous extracts from the haulm and flower of Gentiana asclepiadea exhibited free radical scavenging and protective (antigenotoxic) effect against DNA oxidation induced by H(2)O(2) in human lymphocytes and human embryonic kidney cells (HEK 293). All four extracts exhibited high scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals at concentrations 2.5 and 25 mg ml(-1). The level of DNA damage was measured using the alkaline version of single-cell gel electrophoresis (comet assay). Challenge with H(2)O(2) shows that the pre-treatment of the cells with non-genotoxic doses of Gentiana extracts protected human DNA-either eliminated or significantly reduced H(2)O(2) induced DNA damage. The genotoxic activity of H(2)O(2) was most effectively decreased after 30 min of pre-incubation with 0.05 mg ml(-1) (range, 93.5%-96.3% of reduction in lymphocytes) and 0.25 mg ml(-1) (range, 59.5%-71.4% and 52.7%-66.4% of reduction in lymphocytes and HEK 293 cells, respectively) of G. asclepiadea extracts. These results suggest that the tested G. asclepiadea extracts could be considered as an effective natural antioxidant source.
authors: Department of Genetics, Comenius University, Bratislava, Slovakia.
authors: Alexandra
authors: A
authors: Hudecová
authors: Katarína
authors: K
authors: Hašplová
authors: Eva
authors: E
authors: Miadoková
authors: Zuzana
authors: Z
authors: Magdolenová
authors: Alessandra
authors: A
authors: Rinna
authors: Andrew R
authors: AR
authors: Collins
authors: Eliška
authors: E
authors: Gálová
authors: Dagmar
authors: D
authors: Vaculčíková
authors: Fridrich
authors: F
authors: Gregáň
authors: Mária
authors: M
authors: Dušinská
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D030012:Gentiana / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents