title: Dextrose prolotherapy and corticosteroid injection into rat Achilles tendon.
abstract: OBJECTIVE
  To assess the mechanical behavior and the histology of collagen fibers after prolotherapy with 12.5% dextrose into rat Achilles tendons and to compare with those of corticosteroid treatment.
  
  
  METHODS
  Out of 60 adult female Wistar rats (70 tendons), 15 received 12.5% dextrose (group I); 15 were treated with corticosteroid injection (group II); and 15 were given 0.9% saline injection (group III), all into the right Achilles tendon, whereas 13 animals received no injections (group IV). Three doses of each substance (groups I, II, and III) were given at a 5-day interval. Collagen fiber color was quantitatively assessed in three samples from each group and in five samples from the control group using picrosirius red staining under polarized and nonpolarized light. Twelve tendons from each group treated with the test substance and 20 tendons from the control group were submitted to the tensile strength test.
  
  
  RESULTS
  There was no statistical difference across the groups with respect to maximum load at failure (n.s.) and absorbed energy (n.s.). With respect to tendon rupture, there was no difference between the myotendinous and the tendinous regions (n.s.). However, hematoxylin-eosin staining revealed statistical significance in lymphocytic inflammatory infiltrate (P = 0.008) and in parallel fiber orientation (P = 0.003) when comparing groups to the control group, without significance for either neovascularization (n.s.) or the presence of fibroblasts (n.s.). Likewise, there was no significant difference between the percentage of mature (n.s.) and immature (n.s.) fibers.
  
  
  CONCLUSIONS
  Dextrose was not deleterious to the tendinous tissue, as it did not change the mechanical and histological properties of Achilles tendons in rats. The data obtained in this study may help clinicians in their daily work as they suggest that injections of 12.5% dextrose caused no harm to the tendons, although the clinical importance in humans still needs to be defined.
authors: Orthopedic Hospital of Passo Fundo, Av. Sete de Setembro, n. 817, Bairro Centro, Passo Fundo, RS, CEP 99010-121, Brazil. caqm37@hotmail.com
authors: C A Q
authors: CA
authors: Martins
authors: R T
authors: RT
authors: Bertuzzi
authors: R A
authors: RA
authors: Tisot
authors: A F
authors: AF
authors: Michelin
authors: J M
authors: JM
authors: do Prado
authors: A
authors: A
authors: Stroher
authors: M
authors: M
authors: Burigo
mesh_terms: D000125:Achilles Tendon / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001696:Biomechanical Phenomena; D004334:Drug Administration Schedule; D005260:Female; D024022:Fibrillar Collagens / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D007267:Injections; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength; D016474:Weight-Bearing
chemical_list: D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents; D024022:Fibrillar Collagens; D005947:Glucose