title: Transmission electron microscopy of bone.
abstract: This chapter describes procedures to process mineralized tissues obtained from different sources for transmission electron microscopy (TEM). Methods for fixation, resin embedding, staining of semi-thin sections and ultrathin sections are presented. In addition, attention will be paid to processing of cultured bone explants for TEM analysis.
authors: Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands. v.everts@acta.nl
authors: Vincent
authors: V
authors: Everts
authors: Anneke
authors: A
authors: Niehof
authors: Wikky
authors: W
authors: Tigchelaar-Gutter
authors: Wouter
authors: W
authors: Beertsen
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000648:ultrastructure*; D006801:Humans; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008867:Microtomy / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods