title: Efficient conversion of 1,2-butanediol to (R)-2-hydroxybutyric acid using whole cells of Gluconobacter oxydans.
abstract: (R)-2-Hydroxybutyric acid ((R)-2-HBA) is an important building block for azinothricin family of antitumour antibiotics and biodegradable poly(2-hydroxybutyric acid). However, optically active (R)-2-HBA could not be produced through microbial fermentation or chemical synthesis. Several biocatalytic methods have been reported for the production of (R)-2-HBA. Those processes used expensive and scarce substrates and would not be suitable for practical application. In this work, Gluconobacter oxydans DSM 2003 was confirmed to have the ability to produce (R)-2-HBA from 1,2-butanediol, a non-toxic and inexpensive compound that had a great potential for biotechnological processes. Under the optimal conditions, the biocatalytic process produced (R)-2-HBA at a high concentration (18.5 g l(-1)) and a high enantiomeric excess (99.7%). The biocatalysis process introduced in this study may provide a technically and economically interesting route for production of (R)-2-HBA.
authors: State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China.
authors: Chao
authors: C
authors: Gao
authors: Wen
authors: W
authors: Zhang
authors: Yuanli
authors: Y
authors: Huang
authors: Cuiqing
authors: C
authors: Ma
authors: Ping
authors: P
authors: Xu
mesh_terms: D055162:Biocatalysis; D001711:Biotransformation; D002072:Butylene Glycols / Q000378:metabolism*; D020600:Gluconobacter oxydans / Q000166:cytology* / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002072:Butylene Glycols; D006885:Hydroxybutyrates; C031570:2-hydroxybutyric acid