title: Multimodality functional imaging of spontaneous canine tumors using 64Cu-ATSM and 18FDG PET/CT and dynamic contrast enhanced perfusion CT.
abstract: OBJECTIVE
  To compare the distribution and uptake of the hypoxia tracer (64)Cu-diacetyl-bis(N(4)-methylthiosemicarbazone) ((64)Cu-ATSM) PET/CT, FDG PET/CT and dynamic contrast enhanced perfusion CT (DCE-pCT) in spontaneous canine tumors. In addition (64)Cu-ATSM distribution over time was evaluated.
  
  
  METHODS
  Nine spontaneous cancer-bearing dogs were prospectively enrolled. FDG (1h pi.) and (64)Cu-ATSM (3 and 24h pi.) PET/CT were performed over three consecutive days. DCE-pCT was performed on day 2. Tumor uptake of FDG and (64)Cu-ATSM was assessed semi-quantitatively and the distribution of FDG, (64)Cu-ATSM and CT perfusion parameters correlated.
  
  
  RESULTS
  (64)Cu-ATSM distribution on scans performed 24h apart displayed moderate to strong correlation; however, temporal changes were observed. The spatial distribution pattern of (64)Cu-ATSM between scans was moderately to strongly positively correlated to FDG, whereas the correlation of CT perfusion parameters to FDG and to (64)Cu-ATSM yielded more varying results.
  
  
  CONCLUSIONS
  (64)Cu-ATSM uptake was positively correlated to FDG. (64)Cu-ATSM was found to be relatively stable between PET scans performed at different time points, important temporal changes were however observed in hypo-perfused regions. These findings potentially indicate that prolonged uptake periods for (64)Cu-ATSM imaging may be needed. Although a moderate to strong correlation between (64)Cu-ATSM and FDG PET/CT is observed, the two tracers provide different biological information with an overlapping spatial distribution.
authors: Department of Small Animal Clinical Sciences, University of Copenhagen, Frederiksberg, Denmark. aeha@life.ku.dk
authors: Anders E
authors: AE
authors: Hansen
authors: Annemarie T
authors: AT
authors: Kristensen
authors: Ian
authors: I
authors: Law
authors: Fintan J
authors: FJ
authors: McEvoy
authors: Andreas
authors: A
authors: Kjær
authors: Svend A
authors: SA
authors: Engelholm
mesh_terms: D000818:Animals; D003287:Contrast Media; D056831:Coordination Complexes; D003301:Copper Radioisotopes; D004283:Dog Diseases / Q000000981:diagnostic imaging*; D004285:Dogs; D019788:Fluorodeoxyglucose F18; D064847:Multimodal Imaging / Q000379:methods*; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000662:veterinary*; D009942:Organometallic Compounds; D055420:Perfusion Imaging / Q000379:methods*; D049268:Positron-Emission Tomography; D011856:Radiographic Image Enhancement / Q000379:methods*; D019275:Radiopharmaceuticals; D013882:Thiosemicarbazones; D014057:Tomography, X-Ray Computed
chemical_list: D003287:Contrast Media; D056831:Coordination Complexes; D003301:Copper Radioisotopes; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D013882:Thiosemicarbazones; C089548:copper (II) diacetyl-di(N(4)-methylthiosemicarbazone); D019788:Fluorodeoxyglucose F18