title: Chemokine ligand Xenopus CXCLC (XCXCLC) regulates cell movements during early morphogenesis.
abstract: We cloned the gene for the CXC-type chemokine ligand, Xenopus CXCLC (XCXCLC), the transcripts of which were detected at the dorsal midline during the gastrula and neurula stages. XCXCLC overexpression resulted in the attraction of nearby mesodermal cells, and the excess of chemoattractant interfered with convergent and extension movements. The direction of the deep neural plate cells around the notoplate was also controlled by XCXCLC. Fluorescence signals for XCXCLC + enhanced green fluorescent protein derivatives accumulated around the notochord region. These results indicate that XCXCLC attracts adjacent cells to the midline region, so as to ensure accurate lateral-medial directional tissue convergence during gastrulation and neurulation.
authors: ICORP Organ Regeneration Project, Japan Science and Technology Agency (JST), Tokyo 153-8902, Japan.
authors: Toshiyasu
authors: T
authors: Goto
authors: Makoto
authors: M
authors: Asashima
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism*; D054262:Gastrulation; D049452:Green Fluorescent Proteins; D009024:Morphogenesis; D054258:Neural Plate / Q000254:growth & development; D054261:Neurulation; D012333:RNA, Messenger / Q000235:genetics; D019707:Receptors, Chemokine / Q000378:metabolism; D015398:Signal Transduction; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
chemical_list: C571175:CXCLC protein, Xenopus; D019743:Chemokines, CXC; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D029867:Xenopus Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins