title: UXT-V1 facilitates the formation of MAVS antiviral signalosome on mitochondria.
abstract: Virus infection induces the MAVS-TNFR-associated factor (TRAF) 3 signaling axis on mitochondria. It remains to elucidate the corresponding regulatory processes. In this study, we identify UXT-V1 as a novel TRAF3-binding protein. UXT-V1 is critical for the virus-induced activation of NF-κB and IFN regulatory factor 3. Reduction of UXT-V1 impairs the induction of IFN-β and attenuates the host antiviral responses. The N-terminal TRAF-binding motif of UXT-V1 binds to the C-terminal TRAF domain of TRAF3, thus facilitating the interaction between TRAF3 and MAVS. Notably, TRAF3 and TNFR-associated death domain protein are recruited onto mitochondria upon virus infection. These translocations are blocked when knocking down UXT-V1. Thus, UXT-V1 represents a novel integral component of the MAVS signalosome on mitochondria, mediating the innate antiviral signal transduction.
authors: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors: Yuefeng
authors: Y
authors: Huang
authors: Heng
authors: H
authors: Liu
authors: Rui
authors: R
authors: Ge
authors: Yi
authors: Y
authors: Zhou
authors: Xiwen
authors: X
authors: Lou
authors: Chen
authors: C
authors: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020816:Amino Acid Motifs; D000818:Animals; D018797:Cell Cycle Proteins; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014777:Virus Diseases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D050838:Interferon Regulatory Factor-3; D016899:Interferon-beta; D018832:Molecular Chaperones; D016328:NF-kappa B; D009363:Neoplasm Proteins; D048008:TNF Receptor-Associated Factor 3; C512612:MAVS protein, mouse; C494232:IRF3 protein, human; C494233:Irf3 protein, mouse; C505371:MAVS protein, human; C118920:UXT protein, human; C568328:UXT protein, mouse