title: Unmasking a temperature-dependent effect of the P. anserina i-AAA protease on aging and development.
abstract: Different molecular pathways involved in maintaining mitochondrial function are of fundamental importance to control cellular homeostasis. Mitochondrial i-AAA protease is part of such a surveillance system and PaIAP is the putative ortholog in the fungal aging model Podospora anserina. Here we investigated the role of PaIAP in aging and development. Deletion of the gene encoding PaIAP resulted in a specific phenotype. When incubated at 27°C, spore germination and fruiting body formation are not different from that of the corresponding wild-type strain. Unexpectedly, the lifespan of the deletion strain is strongly increased. In contrast, cultivation at an elevated temperature of 37°C leads to impairments in spore germination and fruiting body formation, and to a reduced lifespan. The higher PaIAP abundance in wild-type strains of the fungus grown at elevated temperature and the phenotype of the deletion strain unmasks a temperature-related role of the protein. The protease appears to be part of a molecular system that has evolved to allow survival under changing temperatures as they characteristically occur in nature.
authors: Goethe University, Faculty for Biosciences & Cluster of Excellence Macromolecular Complexes Frankfurt, Institute of Molecular Biosciences, Frankfurt, Germany.
authors: Andrea
authors: A
authors: Weil
authors: Karin
authors: K
authors: Luce
authors: Stefan
authors: S
authors: Dröse
authors: Ilka
authors: I
authors: Wittig
authors: Ulrich
authors: U
authors: Brandt
authors: Heinz D
authors: HD
authors: Osiewacz
mesh_terms: D049069:ATP-Dependent Proteases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000220:Adaptation, Biological / Q000235:genetics / Q000502:physiology; D000375:Aging / Q000502:physiology*; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006706:Homeostasis / Q000502:physiology*; D008136:Longevity / Q000235:genetics / Q000502:physiology; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010101:Oxygen Consumption / Q000502:physiology; D048468:Podospora / Q000201:enzymology* / Q000254:growth & development; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000254:growth & development; D018709:Statistics, Nonparametric; D013696:Temperature
chemical_list: D009841:Oligonucleotides; D049069:ATP-Dependent Proteases