title: Decoding cell lineage from acquired mutations using arbitrary deep sequencing.
abstract: Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.
authors: Department of Pathology, University of Washington School of Medicine, Seattle, Washington, USA.
authors: Cheryl A
authors: CA
authors: Carlson
authors: Arnold
authors: A
authors: Kas
authors: Robert
authors: R
authors: Kirkwood
authors: Laura E
authors: LE
authors: Hays
authors: Bradley D
authors: BD
authors: Preston
authors: Stephen J
authors: SJ
authors: Salipante
authors: Marshall S
authors: MS
authors: Horwitz
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics*; D003198:Computer Simulation; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D051379:Mice; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*