title: Establishment of an embryonic stem cell line from blastocyst stage mouse embryos.
abstract: Embryonic stem cells have the ability to remain undifferentiated and proliferate in vitro while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. The aim of the present study was to establish mouse ES lines from blastocyst stage embryos obtained after CD1/EGFP mice superovulation. We isolated, cultured and determined the characteristics of mouse embryonic stem cells in early passages, which were first described by Evans M and Kaufman M. Therefore, we evaluated the morphological criteria for the approval of ES cells in early expansion stage. Two cell lines were isolated (CDE1 and CDE2) and analyzed. They showed similar characteristics to those reported earlier for blastocyst-derived ES cell lines.
authors: Department of Veterinary Reproduction, Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania. pallemoke@gmail.com
authors: Emoke
authors: E
authors: Páll
authors: I
authors: I
authors: Groza
authors: M
authors: M
authors: Cenariu
authors: Olga
authors: O
authors: Soriţău
authors: Elen
authors: E
authors: Gócza
authors: C
authors: C
authors: Tomuleasa
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line / Q000166:cytology*; D048430:Cell Shape; D003114:Colony-Forming Units Assay; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000201:enzymology; D061252:Feeder Cells / Q000166:cytology; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase