title: The nuclear pore complex mediates binding of the Mig1 repressor to target promoters.
abstract: All eukaryotic cells alter their transcriptional program in response to the sugar glucose. In Saccharomyces cerevisiae, the best-studied downstream effector of this response is the glucose-regulated repressor Mig1. We show here that nuclear pore complexes also contribute to glucose-regulated gene expression. NPCs participate in glucose-responsive repression by physically interacting with Mig1 and mediating its function independently of nucleocytoplasmic transport. Surprisingly, despite its abundant presence in the nucleus of glucose-grown nup120Δ or nup133Δ cells, Mig1 has lost its ability to interact with target promoters. The glucose repression defect in the absence of these nuclear pore components therefore appears to result from the failure of Mig1 to access its consensus recognition sites in genomic DNA. We propose that the NPC contributes to both repression and activation at the level of transcription.
authors: Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi, United States of America.
authors: Nayan J
authors: NJ
authors: Sarma
authors: Thomas D
authors: TD
authors: Buford
authors: Terry
authors: T
authors: Haley
authors: Kellie
authors: K
authors: Barbara-Haley
authors: George M
authors: GM
authors: Santangelo
authors: Kristine A
authors: KA
authors: Willis
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D043324:beta-Fructofuranosidase / Q000235:genetics
chemical_list: D004271:DNA, Fungal; C085976:MIG1 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D021122:Protein Subunits; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase; D005947:Glucose