title: Studying gene expression in bone by in situ hybridization.
abstract: Here, we described a method for carrying out nonradioactive in situ hybridization to detect mRNA transcripts in cryostat sections of mouse bone using the CryoJane(®) Tape-Transfer System and digoxigenin (DIG)-labeled riboprobes.
authors: Musculoskeletal Disease Department, Novartis Institutes for BioMedical Research, Basel, Switzerland. ina.kramer@novartis.com
authors: Ina
authors: I
authors: Kramer
authors: Rishard
authors: R
authors: Salie
authors: Mira
authors: M
authors: Susa
authors: Michaela
authors: M
authors: Kneissel
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D016616:Cryoultramicrotomy / Q000379:methods; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D012333:RNA, Messenger