title: Diffractaic acid, a novel proapoptotic agent, induces with olive oil both apoptosis and antioxidative systems in Ti-implanted rabbits.
abstract: Usnea longissima Ach., a lichen species, is a traditional herbal medicine with anti-detrimental effects. We evaluated the in vivo effects of a major constituent of U. longissima, diffractaic acid, and the main fatty component of the Mediterranean diet, olive oil, against apoptosis, including various caspase activations and oxidative injury in surrounding tissues after titanium implantation in rabbit femurs. Furthermore, we evaluated the underlying molecular mechanisms. In this study, this lichen metabolite and olive oil activated caspase-dependent cell death with apoptotic morphology, which is distinctly different from necrosis. Both orally and locally administered olive oil and diffractaic acid exerted pro-apoptotic induction in tissues surrounding the implants in titanium-implanted rabbits through the activation of initiator caspases (Cas-2, -8 and -9) and executioner caspase (Cas-3). In addition, they displayed strong myeloperoxidase and inducible nitric oxide synthase activities, providing an alleviating effect. Furthermore, administrations of diffractaic acid and olive oil attenuated the Ti-alloy implantation, and decreased superoxide dismutase activity and total glutathione level in peri-implant tissues. These results demonstrate that diffractaic acid and olive oil are involved in the induction of apoptotic cell death both through caspase-dependent cell death and as an antioxidant. Thus, the data suggest that both diffractaic acid and olive oil could be developed as effective proapoptotic agents in various disorders treatments.
authors: Ataturk Univ., Faculty of Pharmacy, Dep. of Biochemistry, Türkiye.
authors: Fehmi
authors: F
authors: Odabasoglu
authors: Omer S
authors: OS
authors: Yildirim
authors: Hayati
authors: H
authors: Aygun
authors: Zekai
authors: Z
authors: Halici
authors: Mesut
authors: M
authors: Halici
authors: Fazli
authors: F
authors: Erdogan
authors: Elif
authors: E
authors: Cadirci
authors: Ahmet
authors: A
authors: Cakir
authors: Zafer
authors: Z
authors: Okumus
authors: Bunyamin
authors: B
authors: Aksakal
authors: Ali
authors: A
authors: Aslan
authors: Deniz
authors: D
authors: Unal
authors: Yasin
authors: Y
authors: Bayir
mesh_terms: D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002452:Cell Count; D005978:Glutathione / Q000378:metabolism; D062385:Hydroxybenzoates / Q000494:pharmacology*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D019736:Prostheses and Implants / Q000009:adverse effects*; D011817:Rabbits; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000009:adverse effects*
chemical_list: D000840:Anisoles; D000975:Antioxidants; D062385:Hydroxybenzoates; D000069463:Olive Oil; D010938:Plant Oils; C093528:diffractaic acid; D014025:Titanium; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D020169:Caspases; D005978:Glutathione