title: Production of resveratrol from p-coumaric acid in recombinant Saccharomyces cerevisiae expressing 4-coumarate:coenzyme A ligase and stilbene synthase genes.
abstract: Resveratrol is a well-known polyphenol present in red wine and exerts antioxidative and anti-carcinogenic effects on the human body. To produce resveratrol in a food-grade yeast, the 4-coumarate:coenzyme A ligase gene (4CL1) from Arabidopsis thaliana and stilbene synthase gene (STS) from Arachis hypogaea were cloned and transformed into Saccharomyces cerevisiae W303-1A. The resveratrol produced was unglycosylated and secreted into the culture medium. A batch culture with 15.3mg/l p-coumaric acid used as precursor resulted in the production of 3.1mg/l resveratrol with 14.4 mol% yield. Deletion of the putative phenyl acrylic acid decarboxylase gene (PAD1) did not enhance resveratrol production.
authors: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
authors: So-Yeon
authors: SY
authors: Shin
authors: Nam Soo
authors: NS
authors: Han
authors: Yong-Cheol
authors: YC
authors: Park
authors: Myoung-Dong
authors: MD
authors: Kim
authors: Jin-Ho
authors: JH
authors: Seo
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics; D010367:Arachis / Q000201:enzymology / Q000235:genetics; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D003470:Culture Media; D006801:Humans; D011422:Propionates; D011995:Recombination, Genetic; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003373:Coumaric Acids; D003470:Culture Media; D011422:Propionates; D013267:Stilbenes; D000217:Acyltransferases; C022674:stilbene synthase; C511052:4CL1 protein, Arabidopsis; D003066:Coenzyme A Ligases; C495469:p-coumaric acid; D000077185:Resveratrol