title: Effect of leaf essential oil of Coccinia indica on egg hatchability and different larval instars of malarial mosquito Anopheles stephensi.
abstract: OBJECTIVE
  To assess the larvicidal and egg hatching inhibition property of the leaf essential oil of Coccinia indica (C. indica) against Anopheles stephensi (An. stephensi).
  
  
  METHODS
  The larvicidal potential of C. indica leaf essential oil was evaluated against 1st, 2nd, 3rd and 4th instars larvae of An. stephensi using WHO protocol. The 24h LC(50) and LC(90) values of the essential oil were determined following probit analysis. The egg hatching inhibition activity was also tested at 10, 20, 40, and 60 mg/L. The IC(50) value of essential oil was determined against eggs of An. stephensi.
  
  
  RESULTS
  The essential oil extracted from C. indica possessed excellent larvicidal and egg hatching inhibition activity against An. stephensi. The bioassays showed LC(50)-LC(90) of 54.3-140.3, 65.5-155.6, 86.8-180.7 and 95.3-192.6 for 1st, 2nd, 3rd, and 4th larval instars, respectively. The 50% egg hatching inhibition concentration (IC(50)) was noted at 16.5 mg/L.
  
  
  CONCLUSIONS
  The present finding suggest that the C. indica leaf essential oil provided an excellent potential for controlling An. stephensi mosquito at earlier stage of their life cycle.
authors: Department of Zoology and Wildlife Biology, AVC College, Tamil Nadu, India. drrajvec@rediffmail.com
authors: Sankaran
authors: S
authors: Rajkumar
authors: Arulsamy
authors: A
authors: Jebanesan
authors: Rajarathinavelu
authors: R
authors: Nagarajan
mesh_terms: D000818:Animals; D000852:Anopheles; D019662:Cucurbitaceae; D005260:Female; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva; D008288:Malaria / Q000517:prevention & control*; D009032:Mosquito Control / Q000379:methods*; D009822:Oils, Volatile / Q000494:pharmacology*; D018515:Plant Leaves; D010938:Plant Oils / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D009822:Oils, Volatile; D010938:Plant Oils