title: The double immunostaining of CD133 and Ki-67 favours a significant co-localization pattern in fibroblastic subtype of meningiomas.
abstract: OBJECTIVE
  A unique molecular and/or cellular marker for meningiomas, the most common intracranial tumours, has not been identified yet.
  
  
  METHODS
  We investigated the co-localization fraction of CD133/Ki-67 in meningioma tissue array slide composed of 80 meningioma tissue samples of various histological variants. CD133 - a cell membrane stem cell marker - was previously proved to be associated with the initiation and progression of intracerebral gliomas and medulloblastomas.
  
  
  RESULTS
  Immunohistochemical co-localization of CD133/Ki-67 was significantly higher in fibroblastic variant than in meningothelial and transitional subtypes. However, since there were only 3 atypical and 1 malignant meningioma spots in the tumour tissue array slide, it is difficult to draw a firm conclusion regarding the actual co-localization percentage and persistence of CD133/Ki-67 in atypical and malignant meningiomas.
  
  
  CONCLUSIONS
  Far higher co-staining percentage of CD133/ Ki-67 in fibroblastic meningioma samples compared to meningothelial subtype, a histological meningioma variant, architectonically resembling the non-neoplastic meningeal cells, gave us the impression that CD133 may play a role in the formation and progression of fibroblastic meningioma variants. The persistency and the validity of this finding need to be verified by further histopathological and molecular research in order to clarify the possible role of CD133 in meningiogenesis.
authors: Department of Neurosurgery, Suleyman Demirel University, Isparta, Turkey. serdarbaki@gmail.com
authors: Baki S
authors: BS
authors: Albayrak
authors: Ozgur
authors: O
authors: Ismailoglu
authors: Gamze
authors: G
authors: Tanriover
authors: Eralp N
authors: EN
authors: Cetinalp
authors: Necdet
authors: N
authors: Demir
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology*; D006023:Glycoproteins / Q000032:analysis*; D006393:Hemangiopericytoma / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008577:Meningeal Neoplasms / Q000737:chemistry / Q000473:pathology*; D008579:Meningioma / Q000737:chemistry / Q000473:pathology*; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D019394:Ki-67 Antigen; C000606023:PROM1 protein, human; D010455:Peptides