title: Spectroscopic, catalytic and binding properties of Bacillus subtilis NO synthase-like protein: comparison with other bacterial and mammalian NO synthases.
abstract: Genome sequencing has shown the presence of genes coding for NO-synthase (NOS)-like proteins in bacteria. The roles and properties of these proteins remain unclear. UV-visible spectroscopy was used to characterize the recombinant NOS-like protein from Bacillus subtilis (bsNOS) in its ferric and ferrous states in the presence of various Fe(III)- and Fe(II)-heme-ligands and of a series of L-arginine (L-arg) analogs. BsNOS exhibited several spectroscopic and binding properties in common with Bacillus anthracis NOS (baNOS) that were clearly different from those of tetrahydrobiopterin (H4B)-free mammalian NOS oxygenase domains (mNOS(oxys)) and of Staphylococcus aureus NOS (saNOS). Interestingly, bsNOS and baNOS that do not contain H4B exhibited properties much closer to those of H4B-containing mNOS(oxys). Moreover, bsNOS was found to efficiently catalyze the oxidation of L-arginine into L-citrulline by H(2)O(2), whereas H4B-free mNOS(oxys) exhibited low activities for this reaction.
authors: Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Université Paris Descartes, UMR 8601 CNRS, Paris, France.
authors: Isabelle
authors: I
authors: Salard-Arnaud
authors: Dennis
authors: D
authors: Stuehr
authors: Jean-Luc
authors: JL
authors: Boucher
authors: Daniel
authors: D
authors: Mansuy
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D055162:Biocatalysis; D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D002956:Citrulline / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D007093:Imidazoles / Q000378:metabolism; D007700:Kinetics; D008322:Mammals / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013045:Species Specificity; D013053:Spectrophotometry
chemical_list: D001426:Bacterial Proteins; D005290:Ferric Compounds; D005296:Ferrous Compounds; D007093:Imidazoles; D011994:Recombinant Proteins; D001708:Biopterins; D002956:Citrulline; D006418:Heme; D001120:Arginine; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; C003402:sapropterin