title: Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage.
abstract: Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. In this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). The GEBd was prepared by fractionated percolation using propylene glycol as solvent. The total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis.
authors: Centro Interdisciplinar de Investigação Bioquímica (CIIB), Universidade de Mogi das Cruzes (UMC), Brazil.
authors: Natalia S S
authors: NS
authors: Guimarães
authors: Joyce C
authors: JC
authors: Mello
authors: Juliana S
authors: JS
authors: Paiva
authors: Paula C P
authors: PC
authors: Bueno
authors: Andresa A
authors: AA
authors: Berretta
authors: Ricardo J
authors: RJ
authors: Torquato
authors: Iseli L
authors: IL
authors: Nantes
authors: Tiago
authors: T
authors: Rodrigues
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D031175:Baccharis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011429:Propolis / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D008055:Lipids; D011429:Propolis; D005978:Glutathione