title: Characterization of the human primary visual cortex and cerebellum proteomes using shotgun mass spectrometry-data-independent analyses.
abstract: We present the first characterization of the human occipital lobe (primary visual cortex) and cerebellum proteomes. Proteins were identified using a combination of gel electrophoresis and data-independent nanoflow liquid chromatography mass spectrometry (nLC-MS(E) ). The resulting data sets comprised 391 and 330 unique proteins in occipital lobe and cerebellum, respectively, present in at least 75% of the analyzed samples with 297 proteins found in common. These proteins have been associated previously with conditions, such as neurological disorder, progressive motor neuropathy, Parkinson's disease and schizophrenia. The unique proteins identified in the occipital lobe included the interesting finding of growth hormone and several members of the Ca²⁺-dependent calmodulin kinase and serine/threonine protein phosphatase families. The complete mapping of these and other brain proteomes may help in the elucidation of neurological processes and identify potential targets for therapeutic strategies.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.forename: Francesca L
authors.initials: FL
authors.lastname: Guest
authors.forename: Corinna
authors.initials: C
authors.lastname: Bauder
authors.forename: Hassan
authors.initials: H
authors.lastname: Rahmoune
authors.forename: Sandra
authors.initials: S
authors.lastname: Pietsch
authors.forename: Sigrum
authors.initials: S
authors.lastname: Roeber
authors.forename: Hans
authors.initials: H
authors.lastname: Kretzschmar
authors.forename: David
authors.initials: D
authors.lastname: Mann
authors.forename: Atik
authors.initials: A
authors.lastname: Baborie
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
mesh_terms: D001931:Brain Mapping; D002531:Cerebellum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D014793:Visual Cortex / Q000737:chemistry*
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome