title: Vitamin E ameliorates ox-LDL-induced foam cells formation through modulating the activities of oxidative stress-induced NF-κB pathway.
abstract: The role of antioxidant supplementation with vitamin E in the prevention of atherosclerosis has been a topic of considerable recent interest. The relevance of vitamin E for macrophage-derived foam cell formation, a hallmark of atherosclerosis, however, has not been unequivocally resolved. Here, we investigated the effect of oxidized LDL (ox-LDL) and vitamin E on lipid accumulation and total cholesterol content in U937 macrophages, reactive oxygen species generation and expression of nuclear factor-κB (NF-κB) signaling pathway. The results showed that the mRNA expression and protein levels of P-selectin were evident in U937 macrophages treated with ox-LDL and vitamin E, which indicating that expression of P-selectin is important in macrophage-derived foam cell formation. Moreover, P-selectin changes in ox-LDL-induced foam cell formation can be mediated by vitamin E through activities of nuclear NF-κB activated by serine phosphorylation of NF-κB inhibitor α, suggesting that activation of NF-κB pathway by macrophages may occur. Taken together, these data suggested that vitamin E can prevent ox-LDL-induced foam cell macrophages formation through modulating the activities of oxidative stress-induced NF-κB pathway.
authors.affiliation: Department of Cardiology, Changzheng Hospital, The Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Huang
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Han
authors.forename: Zong-Gui
authors.initials: ZG
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005487:Foam Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D012694:Serine; D015398:Signal Transduction / Q000187:drug effects*; D014162:Transfection; D020298:U937 Cells; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D020672:I-kappa B Proteins; D008077:Lipoproteins, LDL; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D019007:P-Selectin; D012333:RNA, Messenger; C546937:SELP protein, human; C099252:oxidized low density lipoprotein; D000072000:NF-KappaB Inhibitor alpha; D014810:Vitamin E; D012694:Serine