title: Low molecular weight compounds from Zoanthus sociatus impair insulin secretion via Ca(+2) influx blockade and cause glucose intolerance in vivo.
abstract: Cnidarians comprise a taxon with a high biodiversity of cytolitic, neurotoxic and cardiotoxic compounds, which have not been studied on insulin release. We tested the effect of a crude extract of Zoanthus sociatus (Ellis, 1767) and the low molecular weight fraction of this extract on insulin secretion in isolated rat β-cells and also in a glucose tolerance test in vivo. We observed that the extract inhibited insulin release by reducing the amount secreted by individual β-cells and also by silencing a fraction of the secreting population. This effect coincided with a diminished rise of intracellular Ca(+2) in response to high glucose and high K+ -induced depolarization. Moreover intraperitoneal administration of the low molecular weight fraction produced glucose intolerance in adult rats. The active fraction exhibited molecular weights similar to the neurotoxins described in the phylum. Our results broaden the toxic effects of cnidarian venoms and show evidence of potential modulators of voltage-gated Ca(+2) channels in this group.
authors.affiliation: Instituto de Fisiología Celular, División de Neurociencias, Departamento de Neurodesarrollo y Fisiología, Universidad Nacional Autónoma de México Ciudad Universitaria, Circuito Ext. UNAM, Coyoacán CP04510, México, DF Mexico. cmanlio@email.ifc.unam.mx
authors.forename: Carlos Manlio
authors.initials: CM
authors.lastname: Diaz-Garcia
authors.forename: Carmen
authors.initials: C
authors.lastname: Sanchez-Soto
authors.forename: Deyanira
authors.initials: D
authors.lastname: Fuentes-Silva
authors.forename: Carolina
authors.initials: C
authors.lastname: Leon-Pinzon
authors.forename: Dany
authors.initials: D
authors.lastname: Dominguez-Perez
authors.forename: Carlos
authors.initials: C
authors.lastname: Varela
authors.forename: Adela
authors.initials: A
authors.lastname: Rodriguez-Romero
authors.forename: Olga
authors.initials: O
authors.lastname: Castañeda
authors.forename: Marcia
authors.initials: M
authors.lastname: Hiriart
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002121:Calcium Channel Blockers / Q000378:metabolism*; D002470:Cell Survival; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018149:Glucose Intolerance / Q000139:chemically induced* / Q000473:pathology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D002121:Calcium Channel Blockers; D007328:Insulin