title: Photodynamic hyperthermal therapy with indocyanine green (ICG) induces apoptosis and cell cycle arrest in B16F10 murine melanoma cells.
abstract: We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8515, Japan.
authors.forename: Rozanaliza
authors.initials: R
authors.lastname: Radzi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D017209:Apoptosis; D059447:Cell Cycle Checkpoints / Q000528:radiation effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006358:Hot Temperature; D007208:Indocyanine Green / Q000494:pharmacology*; D008545:Melanoma / Q000628:therapy*; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology
chemical_list: D017319:Photosensitizing Agents; D007208:Indocyanine Green