title: Genetic diversity in the 3'-terminal region of papaya ringspot virus (PRSV-W) isolates from watermelon in Oklahoma.
abstract: The 3'-terminal region (1191 nt) containing part of the NIb gene, complete coat protein (CP) and poly-A tail of 64 papaya ringspot virus (PRSV-W) isolates collected during 2008-2009 from watermelon in commercial fields of four different counties of Oklahoma were cloned and sequenced. Nucleotide and amino acid sequence identities ranged from 95.2-100% and 97.1-100%, respectively, among the Oklahoman PRSV-W isolates. Phylogenetic analysis showed that PRSW-W isolates clustered according to the locations where they were collected within Oklahoma, and each cluster contained two subgroups. All subgroups of Oklahoman PRSV-W isolates were on separate branches when compared to 35 known isolates originating from other parts of the world, including the one reported previously from the USA. This study helps in our understanding about the genetic diversity of PRSV-W isolates infecting cucurbits in Oklahoma.
authors.affiliation: Department of Biological Science, The University of Tulsa, 800 S. Tucker Dr, Tulsa, OK 74104, USA.
authors.forename: Osama A
authors.initials: OA
authors.lastname: Abdalla
authors.forename: Akhtar
authors.initials: A
authors.lastname: Ali
mesh_terms: D029773:Citrullus / Q000821:virology*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009825:Oklahoma; D010802:Phylogeny; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins