title: Influence of drying on the secondary structure of intrinsically disordered and globular proteins.
abstract: Circular dichroism (CD) spectroscopy of five Arabidopsis late embryogenesis abundant (LEA) proteins constituting the plant specific families LEA_5 and LEA_6 showed that they are intrinsically disordered in solution and partially fold during drying. Structural predictions were comparable to these results for hydrated LEA_6, but not for LEA_5 proteins. FTIR spectroscopy showed that verbascose, but not sucrose, strongly affected the structure of the dry proteins. The four investigated globular proteins were only mildly affected by drying in the absence, but strongly in the presence of sugars. These data highlight the larger structural flexibility of disordered compared to globular proteins and the impact of sugars on the structure of both disordered and globular proteins during drying.
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Hundertmark
authors.forename: Antoaneta V
authors.initials: AV
authors.lastname: Popova
authors.forename: Saskia
authors.initials: S
authors.lastname: Rausch
authors.forename: Robert
authors.initials: R
authors.lastname: Seckler
authors.forename: Dirk K
authors.initials: DK
authors.lastname: Hincha
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002942:Circular Dichroism; D003890:Desiccation; D007782:Lactoglobulins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D029681:Arabidopsis Proteins; D002241:Carbohydrates; D007782:Lactoglobulins; D009844:Oligosaccharides; D010940:Plant Proteins; D012996:Solutions; C066246:late embryogenesis abundant protein, plant; D012710:Serum Albumin, Bovine; C003427:thaumatin protein, plant; C570815:verbascose; D012259:Ribonuclease, Pancreatic