title: [The application of xMAP technology in genetic typing of measles virus].
abstract: The genetic typing of measles virus in clinical samples using xMAP technology was applied. The study provided the calculation and application of specific oligonucleotide probes of genotypes D4, D6 and D7 of measles virus. The strain HobO96 genotype A of measles virus as a check sample was used. The technical approaches to the optimization of preparatory work organization and to the process of identification of measles virus genotypes are described. The presence of genotypes D4, D6 and D7 in clinical samples is proved by the sequence analysis. The genetic typing effectiveness of technique of DNA hybridization using xMAP technology on the instrumental base BioPlex (BioRad, USA) is demonstrated.
authors.forename: N L
authors.initials: NL
authors.lastname: Maksimov
authors.forename: A P
authors.initials: AP
authors.lastname: Agafonov
authors.forename: I G
authors.initials: IG
authors.lastname: Drozdov
mesh_terms: D021121:Branched DNA Signal Amplification Assay / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis*; D005838:Genotype; D006801:Humans; D008457:Measles / Q000235:genetics / Q000821:virology; D008459:Measles virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia; D017422:Sequence Analysis, DNA / Q000379:methods; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus