title: Adult cardiac-resident MSC-like stem cells with a proepicardial origin.
abstract: Colony-forming units - fibroblast (CFU-Fs), analogous to those giving rise to bone marrow (BM) mesenchymal stem cells (MSCs), are present in many organs, although the relationship between BM and organ-specific CFU-Fs in homeostasis and tissue repair is unknown. Here we describe a population of adult cardiac-resident CFU-Fs (cCFU-Fs) that occupy a perivascular, adventitial niche and show broad trans-germ layer potency in vitro and in vivo. CRE lineage tracing and embryo analysis demonstrated a proepicardial origin for cCFU-Fs. Furthermore, in BM transplantation chimeras, we found no interchange between BM and cCFU-Fs after aging, myocardial infarction, or BM stem cell mobilization. BM and cardiac and aortic CFU-Fs had distinct CRE lineage signatures, indicating that they arise from different progenitor beds during development. These diverse origins for CFU-Fs suggest an underlying basis for differentiation biases seen in different CFU-F populations, and could also influence their capacity for participating in tissue repair.
authors.affiliation: Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, 2010, Australia.
authors.forename: James J H
authors.initials: JJ
authors.lastname: Chong
authors.forename: Vashe
authors.initials: V
authors.lastname: Chandrakanthan
authors.forename: Munira
authors.initials: M
authors.lastname: Xaymardan
authors.forename: Naisana S
authors.initials: NS
authors.lastname: Asli
authors.forename: Joan
authors.initials: J
authors.lastname: Li
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.forename: Corey
authors.initials: C
authors.lastname: Heffernan
authors.forename: Mary K
authors.initials: MK
authors.lastname: Menon
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Scarlett
authors.forename: Amirsalar
authors.initials: A
authors.lastname: Rashidianfar
authors.forename: Christine
authors.initials: C
authors.lastname: Biben
authors.forename: Hans
authors.initials: H
authors.lastname: Zoellner
authors.forename: Emily K
authors.initials: EK
authors.lastname: Colvin
authors.forename: John E
authors.initials: JE
authors.lastname: Pimanda
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Biankin
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: William T
authors.initials: WT
authors.lastname: Pu
authors.forename: Owen W J
authors.initials: OW
authors.lastname: Prall
authors.forename: Richard P
authors.initials: RP
authors.lastname: Harvey
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006321:Heart / Q000196:embryology / Q000254:growth & development; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D010496:Pericardium / Q000166:cytology*; D018183:Transplantation Chimera
chemical_list: D015415:Biomarkers