title: Imaging, autoradiography, and biodistribution of (188)Re-labeled PEGylated nanoliposome in orthotopic glioma bearing rat model.
abstract: The (188)Re-labeled pegylated nanoliposome (abbreviated as (188)Re-Liposome) was prepared and evaluated for its potential as a theragnostic agent for glioma. (188)Re-BMEDA complex was loaded into the pegylated liposome core with pH 5.5 ammonium sulfate gradient to produce (188)Re-Liposome. Orthotopic Fischer344/F98 glioma tumor-bearing rats were prepared and intravenously injected with (188)Re-Liposome. Biodistribution, pharmacokinetic study, autoradiography (ARG), histopathology, and nano-SPECT/CT imaging were conducted for the animal model. The result showed that (188)Re-Liposome accumulated in the brain tumor of the animal model from 0.28%±0.09% injected dose (ID)/g (n=3) at 1 hour to a maximum of 1.95%±0.35% ID/g (n=3) at 24 hours postinjection. The tumor-to-normal brain uptake ratio (T/N ratio) increased from 3.5 at 1 hour to 32.5 at 24 hours. Both ARG and histopathological images clearly showed corresponding tumor regions with high T/N ratios. Nano-SPECT/CT detected a very clear tumor image from 4 hours till 48 hours. This study reveals the potential of (188)Re-Liposome as a theragnostic agent for brain glioma.
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
authors.forename: Feng-Yun J
authors.initials: FY
authors.lastname: Huang
authors.forename: Te-Wei
authors.initials: TW
authors.lastname: Lee
authors.forename: Chih-Hao K
authors.initials: CH
authors.lastname: Kao
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Chang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhang
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Lee
authors.forename: Wan-Jou
authors.initials: WJ
authors.lastname: Chen
authors.forename: Shu-Chi
authors.initials: SC
authors.lastname: Wang
authors.forename: Jem-Mau
authors.initials: JM
authors.lastname: Lo
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005910:Glioma / Q000000981:diagnostic imaging* / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D053758:Nanoparticles / Q000737:chemistry; D011868:Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D012211:Rhenium / Q000737:chemistry / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed / Q000379:methods; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D008081:Liposomes; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D012211:Rhenium