title: Expression characterization of genes for CMS-C in maize.
abstract: Cytoplasmic male sterility (CMS)-C is one of the most attractive sources of male sterility in the production of hybrid maize. However, the abortion mechanism of CMS-C is currently unknown. The major aim of this work was to characterize the expression of genes and proteins during pollen abortion. The materials assayed included CMS-C line C48-2, its maintainer line N48-2, and fertile F(1) (C48-2 × 18 white). A total of 20 unique genes and 25 proteins were identified by suppression subtractive hybridization and 2-D electrophoresis, respectively. Most of the genes and proteins identified are closely related to energy metabolism, stress responses, molecular chaperones, and cell death, which are generally considered to be essential to pollen development. Based on the function of these identified genes and proteins, reactive oxygen species in isolated mitochondria and DNA fragments were analyzed. The results from this study indicate that the oxidative stress which was associated with the specific expression patterns of some genes may be the physiological cause for the abortion of premature microspores in the maize CMS-C line.
authors.affiliation: Maize Research Institute of Sichuan Agricultural University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education/Key Laboratory of Maize Biology and Genetic Breeding on Southwest, Ministry of Agriculture, Ya'an, 625014, Sichuan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Xiang
authors.forename: Jiazhou
authors.initials: J
authors.lastname: Liu
authors.forename: Tingzhao
authors.initials: T
authors.lastname: Rong
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lu
authors.forename: Qilin
authors.initials: Q
authors.lastname: Tang
authors.forename: Wen
authors.initials: W
authors.lastname: Wen
authors.forename: Moju
authors.initials: M
authors.lastname: Cao
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D051479:Plant Infertility / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism