title: Detection of cytochrome P450 substrates by using a small-molecule droplet array on an NADH-immobilized solid surface.
abstract: Seeing below the surface: A small-molecule droplet array platform on an NADH-immobilized solid surface and a biotinylated acetophenone derivative were developed to identify the substrate candidates for soluble P450 enzymes of interest. This methodology is thought to be easily applicable to other class I P450 systems, including those that use NADPH as cofactor.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takayama
authors.forename: Shunji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriya
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Iwabuchi
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanoh
mesh_terms: D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D009243:NAD; D003577:Cytochrome P-450 Enzyme System