title: Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
abstract: Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons of the substantia nigra pars compacta with unknown aetiology. 6-Hydroxydopamine (6-OHDA) treatment of neuronal cells is an established in vivo model for mimicking the effect of oxidative stress found in PD brains. We examined the effects of 6-OHDA treatment on human neuroblastoma cells (SH-SY5Y) and primary mesencephalic cultures. Using a reverse arbitrarily primed polymerase chain reaction (RAP-PCR) approach we generated reproducible genetic fingerprints of differential expression levels in cell cultures treated with 6-OHDA. Of the resulting sequences, 23 showed considerable homology to known human coding sequences. The results of the RAP-PCR were validated by reverse transcription PCR, real-time PCR and, for selected genes, by Western blot analysis and immunofluorescence. In four cases, [tomoregulin-1 (TMEFF-1), collapsin response mediator protein 1 (CRMP-1), neurexin-1, and phosphoribosylaminoimidazole synthetase (GART)], a down-regulation of mRNA and protein levels was detected. Further studies will be necessary on the physiological role of the identified proteins and their impact on pathways leading to neurodegeneration in PD.
authors.affiliation: Department of Neurology, Philipps-University Marburg, Germany. noelker@staff.uni-marburg.de
authors.forename: Carmen
authors.initials: C
authors.lastname: Noelker
authors.forename: Michael
authors.initials: M
authors.lastname: Schwake
authors.forename: Monika
authors.initials: M
authors.lastname: Balzer-Geldsetzer
authors.forename: Michael
authors.initials: M
authors.lastname: Bacher
authors.forename: Julius
authors.initials: J
authors.lastname: Popp
authors.forename: Juergen
authors.initials: J
authors.lastname: Schlegel
authors.forename: Karla
authors.initials: K
authors.lastname: Eggert
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Oertel
authors.forename: Thomas
authors.initials: T
authors.lastname: Klockgether
authors.forename: Richard C
authors.initials: RC
authors.lastname: Dodel
mesh_terms: D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D006892:Hydroxydopamines; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D020734:Parkinsonian Disorders / Q000139:chemically induced* / Q000378:metabolism*
chemical_list: D006892:Hydroxydopamines; D009419:Nerve Tissue Proteins