title: Cell-free protein synthesis as a promising expression system for recombinant proteins.
abstract: Cell-free protein synthesis (CFPS) has major advantages over traditional cell-based methods in the capability of high-throughput protein synthesis and special protein production. During recent decades, CFPS has become an alternative protein production platform for both fundamental and applied purposes. Using Renilla luciferase as model protein, we describe a typical process of CFPS in wheat germ extract system, including wheat germ extract preparation, expression vector construction, in vitro protein synthesis (transcription/translation), and target protein assay.
authors.affiliation: Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, USA.
authors.forename: Xumeng
authors.initials: X
authors.lastname: Ge
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
mesh_terms: D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics; D049410:Luciferases, Renilla / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*; D014176:Protein Biosynthesis / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry*; D014158:Transcription, Genetic / Q000502:physiology; D014908:Triticum / Q000737:chemistry* / Q000196:embryology
chemical_list: D010936:Plant Extracts; D011994:Recombinant Proteins; D049410:Luciferases, Renilla