title: A surface plasmon resonance sensor for the detection of deoxynivalenol using a molecularly imprinted polymer.
abstract: The aim of the present work was to investigate the feasibility of applying the molecular imprinting polymer technique to the detection of the mycotoxin deoxynivalenol (DON) using a surface plasmon resonance (SPR) transducer. A molecularly imprinted polypyrrole (MIPPy) film was prepared via electropolymerization of pyrrole onto a bare Au chip in the presence of a template DON molecule. Atomic force microscope SPR analysis showed that the MIPPy film was deposited homogeneously on the Au surface, with a thickness of 5 nm. The MIPPy-SPR sensor exhibited a linear response for the detection of DON in the range of 0.1-100 ng/mL (R2 = 0.988). The selectivity efficiency of the MIPPy film for DON and its acetylated analogs 3-ADON and 15-ADON was 100, 19, and 44%, respectively. The limit of detection for DON with the MIPPy-SPR for a standard solution was estimated at >1 ng/mL. These results suggest that the combination of SPR sensing with a MIPPy film as a synthetic receptor can be used to detect DON.
authors.affiliation: Food Safety Research Center, Korea Food Research Institute, Sungnam 463-746, South Korea. swchoi@kfri.re.kr
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Chang
authors.forename: Nari
authors.initials: N
authors.lastname: Lee
authors.forename: Hyang Sook
authors.initials: HS
authors.lastname: Chun
mesh_terms: D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D011108:Polymers; D014255:Trichothecenes; C007262:deoxynivalenol