title: DNA methylation analysis of germ cells by using bisulfite-based sequencing methods.
abstract: Dynamic changes in DNA methylation at the gene-specific and genome-wide level occur during mammalian germ-cell development. However, the details of how the methylation profiles change remain largely unknown. Bisulfite sequencing analysis is a powerful technique to determine the methylation status of DNA at individual cytosine-guanine dinucleotide (CpG) sites and requires only a small amount of DNA for analysis. Here, we introduce two methods for bisulfite-based DNA methylation analyses using small samples such as germ cells: bisulfite Sanger sequencing at a specific locus and high-throughput bisulfite sequencing at the whole genome level.
authors.affiliation: Department of BioScience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Hisato
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kono
mesh_terms: D000818:Animals; D019175:DNA Methylation; D057890:Epigenomics / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005854:Germ Cells / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D013447:Sulfites / Q000737:chemistry*
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite