title: Cultivated single-stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA-binding stains.
abstract: This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.
authors.affiliation: Department of Natural Sciences, Linneaus University, Kalmar, Sweden. k.holmfeldt@gmail.com
authors.forename: Karin
authors.initials: K
authors.lastname: Holmfeldt
authors.forename: Duško
authors.initials: D
authors.lastname: Odić
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Sullivan
authors.forename: Mathias
authors.initials: M
authors.lastname: Middelboe
authors.forename: Lasse
authors.initials: L
authors.lastname: Riemann
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D041963:Bacteroidetes / Q000821:virology*; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000302:isolation & purification*; D008854:Microscopy, Electron; D012623:Seawater / Q000821:virology*; D013194:Staining and Labeling / Q000379:methods; D019562:Viral Load; D014771:Virion / Q000648:ultrastructure; D014773:Virology / Q000379:methods
chemical_list: D004277:DNA, Single-Stranded