title: Capillary electrophoresis of an X-chromosome STR decaplex for kinship deficiency cases.
abstract: During the two last decades, STR markers located on the autosomes have been gaining relevance and have nearly replaced the use of other type of markers in most cases of genetic identification, paternity testing, as well as in other situations of kinship analysis. Nevertheless, in some complex cases, independently of the number of polymorphisms being typed, autosomal markers convey very little information. Depending on the parentage constellation available for analysis, as well as the gender of the subjects, this problem can sometimes be solved by using markers that have different modes of transmission. Therefore, most forensic laboratories are nowadays prepared to analyse lineage markers (Y chromosome and mtDNA) and many have recently set up methods for the analysis of X-STRs. In the present chapter, a method is described for the typing of ten X chromosome-specific markers in a single PCR amplification reaction, followed by capillary electrophoresis separation and fluorescent detection in an ABI Genetic Analyser apparatus. This typing strategy was developed and optimized for the simultaneous amplification of ten X-linked specific STRs well distributed along the chromosome: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08 and DXS7423.
authors.affiliation: IPATIMUP-Institute of Pathology and Molecular Immunology of the University of Porto, Porto, Portugal. lgusmao@ipatimup.pt
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
authors.forename: Cíntia
authors.initials: C
authors.lastname: Alves
authors.forename: Iva
authors.initials: I
authors.lastname: Gomes
authors.forename: Paula
authors.initials: P
authors.lastname: Sánchez-Diz
mesh_terms: D000483:Alleles; D041321:Chromosomes, Human, X / Q000235:genetics*; D041322:Chromosomes, Human, Y / Q000235:genetics; D017931:DNA Primers / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D056426:Genetic Loci / Q000235:genetics; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers