title: SIRT1 and the clock gene machinery in colorectal cancer.
abstract: SIRT1 and the clock genes are involved in carcinogenesis. We evaluated SIRT1 expression in 19 human colorectal cancer (CRC) specimens and clock gene expression in SIRT1-overexpressing CaCo2 and SW480 cells. In CRC, SIRT1 mean expression level was decreased. Compared to CaCo2 cells, SW480 cells displayed lower levels of SIRT1 and PER3 and higher levels of ARNTL1, CLOCK, PER1, PER2, CRY1, TIPIN, and CSNKIE. SIRT1 overexpression induced PER1 upregulation in CaCo2 and downregulation in SW480 cells. SIRT1 expression was heterogeneous in human CRC and in CRC cell lines. These results might have relevant implications for a better understanding of colorectal carcinogenesis.
authors.affiliation: Division and Laboratory of Gastroenterology, IRCCS Casa Sollievo della Sofferenza, Research Hospital, Opera di Padre Pio da Pietrelcina, San Giovanni Rotondo (FG), Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Pazienza
authors.forename: Ada
authors.initials: A
authors.lastname: Piepoli
authors.forename: Anna
authors.initials: A
authors.lastname: Panza
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Valvano
authors.forename: Giorgia
authors.initials: G
authors.lastname: Benegiamo
authors.forename: Manlio
authors.initials: M
authors.lastname: Vinciguerra
authors.forename: Angelo
authors.initials: A
authors.lastname: Andriulli
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Mazzoccoli
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D056926:CLOCK Proteins / Q000096:biosynthesis / Q000235:genetics*; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D056564:Sirtuin 1 / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
chemical_list: D056926:CLOCK Proteins; C447939:SIRT1 protein, human; D056564:Sirtuin 1