title: The effect of acetylated xylan and sugar beet pulp on the expression and secretion of enzymes by Penicillium purpurogenum.
abstract: Sugar beet pulp is a natural carbon source composed mainly of pectin and cellulose, which is utilized and degraded by the ascomycete Penicillium purpurogenum. The fungus also grows on and degrades acetylated xylan which lacks cellulose and pectin. Both carbon sources have been used in our laboratory to grow the fungus and to purify different enzymes secreted to the medium. The enzymes involved in the complex process of degradation of these carbon sources by the fungus have been explored previously under non-denaturing conditions; multienzyme complexes were separated and some subunits identified by Western blots and mass spectrometry. In this work, proteomic profiles show that the secretome is composed of numerous proteins varying in pI and molecular weight. Some enzymes are common to both growth conditions, while others are specific for each carbon source. The results show that the carbon sources utilized exert strong regulatory control over the proteins secreted. This is the first secretome study from a lignocellulolytic Penicillium.
authors.affiliation: Facultad de Ciencias Biológicas, Universidad Andrés Bello, República 217, Santiago, Chile.
authors.forename: Mario
authors.initials: M
authors.lastname: Navarrete
authors.forename: Eduardo
authors.initials: E
authors.lastname: Callegari
authors.forename: Jaime
authors.initials: J
authors.lastname: Eyzaguirre
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry*; D002482:Cellulose / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry; D020869:Gene Expression Profiling; D007526:Isoelectric Point; D008970:Molecular Weight; D010368:Pectins / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D020543:Proteome / Q000032:analysis; D014990:Xylans / Q000378:metabolism*
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D020543:Proteome; D014990:Xylans; D010368:Pectins; D002482:Cellulose