title: Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET.
abstract: Using a single-molecule method, we directly distinguish among oligomers from monomers to tetramers and determine their quaternary structures. Using this method, we found that RecR forms a stable dimer and its oligomeric form is modulated by its own concentration and the interaction with RecO.
authors.affiliation: Department of Physics and School of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Cheolhee
authors.initials: C
authors.lastname: Kim
authors.forename: Jae Yeol
authors.initials: JY
authors.lastname: Kim
authors.forename: Seung Hyeon
authors.initials: SH
authors.lastname: Kim
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Nam Ki
authors.initials: NK
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D034301:Deinococcus; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007834:Lasers; D008958:Models, Molecular; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; D012996:Solutions; C065831:RecR protein, Bacteria