title: The Andes hantavirus NSs protein is expressed from the viral small mRNA by a leaky scanning mechanism.
abstract: The small mRNA (SmRNA) of all Bunyaviridae encodes the nucleocapsid (N) protein. In 4 out of 5 genera in the Bunyaviridae, the smRNA encodes an additional nonstructural protein denominated NSs. In this study, we show that Andes hantavirus (ANDV) SmRNA encodes an NSs protein. Data show that the NSs protein is expressed in the context of an ANDV infection. Additionally, our results suggest that translation initiation from the NSs initiation codon is mediated by ribosomal subunits that have bypassed the upstream N protein initiation codon through a leaky scanning mechanism.
authors.affiliation: Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, Centro de Investigaciones Médicas, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Vera-Otarola
authors.forename: Loretto
authors.initials: L
authors.lastname: Solis
authors.forename: Ricardo
authors.initials: R
authors.lastname: Soto-Rifo
authors.forename: Emiliano P
authors.initials: EP
authors.lastname: Ricci
authors.forename: Karla
authors.initials: K
authors.lastname: Pino
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Tischler
authors.forename: Théophile
authors.initials: T
authors.lastname: Ohlmann
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Darlix
authors.forename: Marcelo
authors.initials: M
authors.lastname: López-Lastra
mesh_terms: D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D006476:Orthohantavirus / Q000235:genetics* / Q000378:metabolism; D018778:Hantavirus Infections / Q000821:virology*; D006801:Humans; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins