title: Long-term preservation, regeneration, and cultivation of Paecilomyces tenuipes (Peck) Samson (Ascomycetes), an entomopathogenic fungus inoculated into the silkworm larva of Bombyx mori.
abstract: Paecilomyces tenuipes reportedly have anticancer and immune activities, along with various other medicinal uses. Cultured products with P. tenuipes are certified for use in food in South Korea, and processed goods containing this fungus have been developed in many countries, particularly South Korea, Japan, and China. Research on mass production technology-procured raw materials for the manufacture of P. tenuipes is very important; however, cultures of the fungus have been unstable. This study identified stable cultivation conditions, focusing on growth inhibition and revitalization. Moisture regulation and preservation of pupae inoculated with P. tenuipes were used to control growth inhibition and revitalization. When inoculated silkworm pupae were dehydrated to 4% moisture and preserved freeze-dried or at -70 degrees C, -20 degrees C, or 4 degrees C, the mycelia in their bodies were able to survive for 14 d. Inoculated silkworm pupae were rehydrated for 3 h and the mycelia within their bodies were recovered at 94.3-96.3%. Silkworm pupae at 4% moisture were able to survive for 135 d at temperatures < 4 degrees C and for 1 y after freeze-drying. Optimal conditions for synnemata induction were 25 degrees C and 100-300 1x.
authors.affiliation: National Academy of Agricultural Science, Department of Agricultural Biology, R.D.A. Suwon, Korea. creative716@korea.kr
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Nam
authors.forename: Chun Ru
authors.initials: CR
authors.lastname: Li
authors.forename: Zeng-Zhi
authors.initials: ZZ
authors.lastname: Li
authors.forename: Mei-Zhen
authors.initials: MZ
authors.lastname: Fan
authors.forename: Seok Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Kwang Gill
authors.initials: KG
authors.lastname: Lee
authors.forename: Joo Hong
authors.initials: JH
authors.lastname: Yeo
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Sang Mi
authors.initials: SM
authors.lastname: Han
authors.forename: Ki Man
authors.initials: KM
authors.lastname: Lee
mesh_terms: D000818:Animals; D012831:Bombyx / Q000382:microbiology*; D048690:Fruiting Bodies, Fungal / Q000254:growth & development* / Q000528:radiation effects; D007814:Larva / Q000382:microbiology; D008027:Light; D025282:Mycelium / Q000254:growth & development*; D010143:Paecilomyces / Q000254:growth & development* / Q000302:isolation & purification / Q000528:radiation effects; D011309:Preservation, Biological / Q000379:methods*; D011679:Pupa / Q000382:microbiology; D013172:Spores, Fungal / Q000254:growth & development; D013696:Temperature