title: Recognition of Hg2+ and Cr3+ in physiological conditions by a rhodamine derivative and its application as a reagent for cell-imaging studies.
abstract: A new rhodamine-based receptor, derivatized with an additional fluorophore (quinoline), was synthesized for selective recognition of Hg(2+) and Cr(3+) in an acetonitrile/HEPES buffer medium of pH 7.3. This reagent could be used as a dual probe and allowed detection of these two ions by monitoring changes in absorption and the fluorescence spectral pattern. In both instances, the extent of the changes was significant enough to allow visual detection. More importantly, the receptor molecule could be used as an imaging reagent for detection of Hg(2+) and Cr(3+) uptake in live human cancer cells (MCF7) using laser confocal microscopic studies. Unlike Hg(ClO(4))(2) or Hg(NO(3))(2) salts, HgCl(2) or HgI(2) failed to induce any visually detectable change in color or fluorescence upon interaction with L(1) under identical experimental conditions. Presumably, the higher covalent nature of Hg(II) in HgCl(2) or HgI(2) accounts for its lower acidity and its inability to open up the spirolactam ring of the reagent L(1). The issue has been addressed on the basis of the single-crystal X-ray structures of L(1)·HgX(2) (X(-) = Cl(-) or I(-)) and results from other spectral studies.
authors.affiliation: Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar 364002, Gujarat, India.
authors.forename: Sukdeb
authors.initials: S
authors.lastname: Saha
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Mahato
authors.forename: Upendar
authors.initials: U
authors.lastname: Reddy G
authors.forename: E
authors.initials: E
authors.lastname: Suresh
authors.forename: Arindam
authors.initials: A
authors.lastname: Chakrabarty
authors.forename: Mithu
authors.initials: M
authors.lastname: Baidya
authors.forename: Sudip K
authors.initials: SK
authors.lastname: Ghosh
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D002857:Chromium / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011804:Quinolines; D012235:Rhodamines; D002857:Chromium; C037219:quinoline; D008628:Mercury