title: Identification of ERp29 as a biomarker for predicting nasopharyngeal             carcinoma response to radiotherapy.
abstract: Radioresistance continues to be a major problem in the treatment of nasopharyngeal             carcinoma (NPC). This study aimed to identify novel proteins associated with NPC             radio-resistance. We used a mass spectrometry driven-proteomic strategy to identify             novel proteins associated with NPC radio-resistance, and differential proteins             were subsequently processed by bio-informatic analysis. As a result, twelve proteins             were identified with aberrant expression in radioresistant (RR) NPC tissues compare             to radiosensitive (RS) NPC tissues. Among these proteins, ERp29, Mn-SOD, HSP27             and GST ω1 were found to be significantly up-regulated in RR NPC tissues, and             ERp29 was selected for further validation. Immunohistochemistry analysis confirmed             that ERp29 was overexpressed in RR NPC tissues compared with RS NPC tissues. To             prove the role of ERp29 in the induction of NPC radioresistance, ERp29 was down-regulated             in the ERp29 enriched NPC cells CNE-1 and 6-10B by specific shRNA. Radiosensitivity             was measured using cell proliferation assay and clonogenic survival assay, and             cell apoptosis was measured using flow cytometric analysis. We found that ERp29             knockdown attenuated CNE-1 and 6-10B cell radioresistance and enhanced cell apoptosis.             These results suggest that ERp29 associates with radioresistance in NPC, and ERp29             could be a potential biomarker for predicting NPC response to radiotherapy.
authors.affiliation: Department of Otolaryngology, Xiangya Hospital, Central South University, Changsha 410008, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Qingping
authors.initials: Q
authors.lastname: Tang
authors.forename: Yaoyun
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Xie
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Lv
authors.forename: Suping
authors.initials: S
authors.lastname: Zhao
authors.forename: Weihong
authors.initials: W
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002681:China; D019295:Computational Biology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D011836:Radiation Tolerance; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C491738:ERP29 protein, human; D006360:Heat-Shock Proteins