title: Effect of varying flow regimes upon elution behaviour, apparent molecular characteristics and hydrodynamic properties of amylopectin isolated from normal corn starch using asymmetrical flow field-flow fractionation.
abstract: A detailed study of the elution behaviour, apparent molecular characteristics and hydrodynamic properties of amylopectin-type fraction (isolated from normal corn starch) in aqueous media employing asymmetrical flow field-flow fractionation (AF4) was undertaken by systematically varying the channel flow (F(ch)), cross flow (F(cr)) and F(cr)/F(ch) ratios. Distributions of apparent molar masses and radii of gyration, mass recoveries and hydrodynamic radii decreased as a function of increasing F(cr) at a fixed F(ch), due to the increase in the retention of amylopectin-type fraction in the AF4 channel. Increased retention of the amylopectin-type fraction in the AF4 channel was also observed at low F(ch) and high F(cr)/F(ch) ratios. Large amylopectin-type molecules/particles (possibly aggregates) eluted at high F(ch), low F(cr) and low F(cr)/F(ch) ratios.
authors.affiliation: NAWI Graz-CePol/MC (Molecular Characteristics), Institute for Chemistry, University of Graz, Graz, Austria. shazia.juna@uni-graz.at
authors.forename: Shazia
authors.initials: S
authors.lastname: Juna
authors.forename: Anton
authors.initials: A
authors.lastname: Huber
mesh_terms: D000687:Amylopectin / Q000737:chemistry* / Q000302:isolation & purification; D000688:Amylose / Q000032:analysis; D031521:Fractionation, Field Flow / Q000379:methods*; D057446:Hydrodynamics; D008872:Microwaves; D008970:Molecular Weight; D011312:Pressure; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000688:Amylose; D000687:Amylopectin