title: The dose-effect of icariin on the proliferation and osteogenic differentiation of human bone mesenchymal stem cells.
abstract: Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10(-9) M to 10(-6) M, but at concentrations above 10(-5) M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.
authors.affiliation: Department of Orthopaedic Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Fan
authors.forename: Liang-Guo
authors.initials: LG
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Long
authors.initials: L
authors.lastname: Bi
authors.forename: Guo-Xian
authors.initials: GX
authors.lastname: Pei
mesh_terms: D000328:Adult; D000880:Anthraquinones / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology*; D002113:Calcification, Physiologic / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D014048:Tolonium Chloride / Q000378:metabolism; D055815:Young Adult
chemical_list: D000880:Anthraquinones; D005419:Flavonoids; D014048:Tolonium Chloride; C010078:alizarin; C056599:icariin