title: [Effects of male worm extraction on ultrastructure of cultured vitelline cells from Schistosoma japonicum].
abstract: OBJECTIVE
  To study the effects of male worm extraction on the proliferation and metabolic activity of cultured vitelline cells from Schistosoma japonicum.
  
  
  METHODS
  The 28-day S. japonicum worms were harvested by perfusion. The male and female of them were isolated after asepsis separately. The vitelline glands of female worms were isolated, and the vitelline cells were harvested by the cold digestion, then they were inoculated with the moist system method on the walls of culture flasks. The cultured vitelline cells were randomly divided into test and control groups. The cells in the control group were cultured in routine media and those in the test group were cultured in routine media containing male worm extraction of the concentration of 100 microg/ml. When cultured for 7 days, the cells in both groups were prepared for observation under a transmission electron microscope.
  
  
  RESULTS
  In the test group, the numbers of mature vitelline cells were more than those in the control group; the cytoplasm and nucleus of mature vitelline cells were homogeneous stain. The nucleolus and rough-surfaced endoplasm reticula were clear, the intervals of vitelline globules were clear and their numbers could be counted. The number of mitochondria was small and the electron density was low; abundant rough-surfaced endoplasm reticula were found in the immature vitelline cells. There were more immature vitelline cells in the control group. The cytoplasm of the cultured vitelline cells took changes of balloon, especially in mature vitelline cells, vitelline globules fused each other, no mitochondria were found; in immature vitelline cells, the space between vitelline globules and the membrane surrounding them broadened gradually and vitelline globules were released and uncovered; rough-surfaced endoplasmic reticula enlarged, space vacuolated and the ribosomes dropped; and the number of lipid increased.
  
  
  CONCLUSIONS
  The S. japonicum male worm extraction can stimulate the development and survival of the cultured vitelline cells.
authors.affiliation: Department of Medical Parasitology and Research Laboratory of Schistosomiasis, School of Basic Medical Science, Wuhan University, Wuhan 430071, China.
authors.forename: Qin-Ping
authors.initials: QP
authors.lastname: Zhong
authors.forename: Jua-Lin
authors.initials: JL
authors.lastname: Li
authors.forename: Zhen-Ping
authors.initials: ZP
authors.lastname: Ming
authors.forename: Ming-Sen
authors.initials: MS
authors.lastname: Jiang
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Dong
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D010063:Ovum / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001685:Biological Factors