title: Wound healing and anti-inflammatory activities of the Michauxia L'Hérit (Campanulaceae) species native to Turkey.
abstract: BACKGROUND
  The leaves of Michauxia species are used for the treatment of wounds in Turkish traditional medicine. In the present study, wound healing, anti-inflammatory and antioxidant activities of the extracts obtained from the root and herb of 5 species of Michauxia collected in different parts of Turkey were evaluated.
  
  
  METHODS
  In vivo incision and excision wound models were used in order to assess the wound healing effects of the methanolic extracts of the plants. Skin samples were also evaluated histopathologically. In vivo inhibitory effect of the extracts on acetic acid-induced increase in capillary permeability was studied for the assessment of anti-inflammatory activity. TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1-picrylhydrazyl) tests were used to evaluate the antioxidant activity.
  
  
  RESULTS
  Noteworthy wound healing activity was observed for the ointment formulation prepared with 1% Michauxia nuda (root) and Michauxia tchihatchewii (herb) extracts. The results of histopathological evaluation supported the outcome of incision and excision wound models. Moreover, the Michauxia nuda (root) exerted remarkable anti-inflammatory effect. The highest antioxidant activity was observed with the ethyl acetate extract of Michauxia tchihatchewii herb.
  
  
  CONCLUSIONS
  The experimental study revealed that Michauxia displays remarkable wound healing and anti-inflammatory and antioxidant activities.
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Tandoğan 06100, Ankara, Turkey.
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Güvenç
authors.forename: Esra Küpeli
authors.initials: EK
authors.lastname: Akkol
authors.forename: M Mesud
authors.initials: MM
authors.lastname: Hürkul
authors.forename: Ipek
authors.initials: I
authors.lastname: Süntar
authors.forename: Hikmet
authors.initials: H
authors.lastname: Keleş
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D029422:Campanulaceae; D002199:Capillary Permeability / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D018517:Plant Roots; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D014421:Turkey; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl; D019342:Acetic Acid