title: Xuhuai goat H-FABP gene clone, subcellular localization of expression products and the preparation of transgenic mice.
abstract: The aim of this study was to clone the heart-type fatty acid binding protein (H-FABP) gene of Xuhuai goat, to explore it bioinformatically, and analyze the subcellular localization using enhanced green fluorescent protein (EGFP). The results showed that the coding sequence (CDS) length of Xuhuai goat H-FABP gene was 402 bp, encoding 133 amino acids (GenBank accession number AY466498.1). The H-FABP cDNA coding sequence was compared with the corresponding region of human, chicken, brown rat, cow, wild boar, donkey, and zebrafish. The similarity were 89%, 76%, 85%, 84%, 93%, 91%, 70%, respectively. For the corresponding amino acid sequences, the similarity were 90%, 79%, 88%, 97%, 95%, 94%, 72%, respectively. This study did not find the signal peptide region in the H-FABP protein; it revealed that H-FABP protein might be a nonsecreted protein. H-FABP expression was detected in vitro by reverse transcription-polymerase chain reaction (RT-PCR), and the EGFP-H-FABP fusion protein was localized to the cytoplasm. The gene could also be transiently and permanently expressed in mice.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Yin
authors.forename: Bi-chun
authors.initials: BC
authors.lastname: Li
authors.forename: Guang-hui
authors.initials: GH
authors.lastname: Wei
authors.forename: Cai-ye
authors.initials: CY
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ya-ni
authors.initials: YN
authors.lastname: Zhang
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Du
authors.forename: Wen-guang
authors.initials: WG
authors.lastname: Cao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006041:Goats; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D015027:Zebrafish
chemical_list: D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins