title: D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants.
abstract: D-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media D-lactate concentrations greater than 5-10mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous D-lactate dehydrogenase (D-LDH) detoxify D-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, D-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on D-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.
authors.affiliation: Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, 40225 Düsseldorf, Germany.
authors.forename: Judith
authors.initials: J
authors.lastname: Wienstroer
authors.forename: Martin K M
authors.initials: MK
authors.lastname: Engqvist
authors.forename: Hans-Henning
authors.initials: HH
authors.lastname: Kunz
authors.forename: Ulf-Ingo
authors.initials: UI
authors.lastname: Flügge
authors.forename: Veronica G
authors.initials: VG
authors.lastname: Maurino
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D017796:Caulimovirus / Q000235:genetics; D003470:Culture Media; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D018525:Germination / Q000235:genetics; D045022:Lactate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D029681:Arabidopsis Proteins; D003470:Culture Media; D005819:Genetic Markers; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase