title: Characterization of novel Leishmania infantum recombinant proteins encoded by genes from five families with distinct capacities for serodiagnosis of canine and human visceral leishmaniasis.
abstract: To expand the available panel of recombinant proteins that can be useful for identifying Leishmania-infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum, cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4-93.0% and 36.4-97.2%) and specificities (76.1-100% and 90.4-97.3%) when tested with serum samples from Leishmania-infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil. ggileno@bahia.fiocruz.br
authors.forename: Geraldo G S
authors.initials: GG
authors.lastname: Oliveira
authors.forename: Franklin B
authors.initials: FB
authors.lastname: Magalhães
authors.forename: Márcia C A
authors.initials: MC
authors.lastname: Teixeira
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Pereira
authors.forename: Cristiane G M
authors.initials: CG
authors.lastname: Pinheiro
authors.forename: Lenita R
authors.initials: LR
authors.lastname: Santos
authors.forename: Marília B
authors.initials: MB
authors.lastname: Nascimento
authors.forename: Cheila N G
authors.initials: CN
authors.lastname: Bedor
authors.forename: Alessandra L
authors.initials: AL
authors.lastname: Albuquerque
authors.forename: Washington L C
authors.initials: WL
authors.lastname: dos-Santos
authors.forename: Yara M
authors.initials: YM
authors.lastname: Gomes
authors.forename: Edson D
authors.initials: ED
authors.lastname: Moreira
authors.forename: Maria E F
authors.initials: ME
authors.lastname: Brito
authors.forename: Lain C
authors.initials: LC
authors.lastname: Pontes de Carvalho
authors.forename: Osvaldo P
authors.initials: OP
authors.lastname: de Melo Neto
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D017125:Genes, Protozoan / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D016547:Kinesins / Q000235:genetics; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000469:parasitology / Q000662:veterinary; D025821:Polyubiquitin / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary
chemical_list: D000953:Antigens, Protozoan; D018840:HSP70 Heat-Shock Proteins; D011994:Recombinant Proteins; D025821:Polyubiquitin; D016547:Kinesins