title: Genus Enhydrobacter Staley et al. 1987 should be recognized as a member of the family Rhodospirillaceae within the class Alphaproteobacteria.
abstract: The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequences of E. aerosaccus-type strain versions NCIMB 12535(T) , ATCC 27094( T) and CCUG 58314(T) were very different from the accessible data (accession no. AJ550856). Phylogenetic analysis of our 16S rRNA sequence data revealed that these organisms were located within the family Rhodospirillaceae. The genera Inquilinus, Oceanibaculum, Skermanella and Nisaea were closely related (sequence similarities were 88.3~87.0%), but Enhydrobacter could be distinguished from these genera by growth characteristics, fatty acid profiles (C(19:0) cyclo ω8c; 38.4% C(18:1) ω7c; 32.2%, and C(16:0) ; 8.9% were major components), in being non-flagellated, and differing in enzymatic activities, including trypsin and β-glucosidase. From these data, we conclude that the genus Enhydrobacter should be recognized as an independent genus of the family Rhodospirillaceae within the class Alphaproteobacteria.
authors.affiliation: Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya 464-8650, Japan. kawamura@dpc.agu.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Nagatoshi
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Takashi
authors.initials: T
authors.lastname: Naka
authors.forename: Asako
authors.initials: A
authors.lastname: Mitani
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kubota
authors.forename: Junko
authors.initials: J
authors.lastname: Tomida
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morita
authors.forename: Jun
authors.initials: J
authors.lastname: Hitomi
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015373:Bacterial Typing Techniques; D004789:Enzyme Activation; D005227:Fatty Acids / Q000378:metabolism; D020459:Genes, rRNA; D044007:Moraxellaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity; D014357:Trypsin / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase; D014357:Trypsin