title: Lentil polyphenol extract prevents angiotensin II-induced hypertension, vascular remodelling and perivascular fibrosis.
abstract: The objective of the study was to investigate whether chronic administration of the Morton lentil polyphenol extract (MLPE), which possesses rich phenolic compounds and a high antioxidant activity, had any protective effects on angiotensin II-induced hypertension. After four weeks of subcutaneous infusion of angiotensin II (200 ng kg(-1) min(-1)) in male SD rats, the water intake and mean artery pressure was significantly increased by 39.8% and 48.3%, respectively, as compared with the control. The media/lumen ratio of the small arteries in the heart and kidneys were increased by 117% and 168% by angiotensin II infusion. The perivascular fibrosis was increased by 65% and 32% in the heart and kidneys, respectively. Levels of the reactive oxygen species in the aorta was enhanced by 115.8%. In another group of rats, which received four weeks of lentil extract administration (1% freeze-dried MLPE in the drinking water), followed by another four weeks of extract administration plus angiotensin II infusion, the angiotensin II-induced enhancement in water intake and mean artery pressures decreased by 12.7% and 8.2%, respectively, as compared with the rats that received angiotensin II infusion alone. The angiotensin II-induced rats showed increases in the media/lumen ratios which were attenuated by 43.6% and 47.2% in the small arteries of heart and kidneys, respectively. Angiotensin II-induced perivascular fibrosis was attenuated by 30% and 26% in the rats that received the extract. Angiotensin II-induced rats showed reactive oxygen species levels in the aorta was reduced by 48.9%. These findings demonstrated that lentil extract attenuated angiotensin II-induced hypertension and associated pathological changes, including remodelling and perivascular fibrosis in the small resistant arteries of heart and kidneys.
authors.affiliation: Department of Food and Cereal Sciences, North Dakota State University, 1320 Albrecht Boulevard, Fargo, ND 58108, USA.
authors.forename: Fanrong
authors.initials: F
authors.lastname: Yao
authors.forename: Chengwen
authors.initials: C
authors.lastname: Sun
authors.forename: Sam K C
authors.initials: SK
authors.lastname: Chang
mesh_terms: D000284:Administration, Oral; D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D001011:Aorta / Q000187:drug effects; D004326:Drinking; D005355:Fibrosis; D006321:Heart / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D007668:Kidney / Q000187:drug effects; D027806:Lens Plant / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D014652:Vascular Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D000804:Angiotensin II