title: Delivery of multiple siRNAs using lipid-coated PLGA nanoparticles for treatment of prostate cancer.
abstract: Nanotechnology can provide a critical advantage in developing strategies for cancer management and treatment by helping to improve the safety and efficacy of novel therapeutic delivery vehicles. This paper reports the fabrication of poly(lactic acid-co-glycolic acid)/siRNA nanoparticles coated with lipids for use as prostate cancer therapeutics made via a unique soft lithography particle molding process called Particle Replication In Nonwetting Templates (PRINT). The PRINT process enables high encapsulation efficiency of siRNA into neutral and monodisperse PLGA particles (32-46% encapsulation efficiency). Lipid-coated PLGA/siRNA PRINT particles were used to deliver therapeutic siRNA in vitro to knockdown genes relevant to prostate cancer.
authors.affiliation: Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
authors.forename: Warefta
authors.initials: W
authors.lastname: Hasan
authors.forename: Kevin
authors.initials: K
authors.lastname: Chu
authors.forename: Anuradha
authors.initials: A
authors.lastname: Gullapalli
authors.forename: Stuart S
authors.initials: SS
authors.lastname: Dunn
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Enlow
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Luft
authors.forename: Shaomin
authors.initials: S
authors.lastname: Tian
authors.forename: Mary E
authors.initials: ME
authors.lastname: Napier
authors.forename: Patrick D
authors.initials: PD
authors.lastname: Pohlhaus
authors.forename: Jason P
authors.initials: JP
authors.lastname: Rolland
authors.forename: Joseph M
authors.initials: JM
authors.lastname: DeSimone
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008297:Male; D053769:Nanocapsules / Q000627:therapeutic use*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000628:therapy*; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use*
chemical_list: D020099:Coated Materials, Biocompatible; D008055:Lipids; D053769:Nanocapsules; D034741:RNA, Small Interfering; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid