title: Biochemical properties of recombinant leucine aminopeptidase II from Bacillus stearothermophilus and potential applications in the hydrolysis of Chinese anchovy (Engraulis japonicus) proteins.
abstract: The effects of various factors on the activity and conformation of recombinant leucine aminopeptidase II (rLAP II) from Bacillus stearothermophilus and its potential utilization in the hydrolysis of anchovy proteins were investigated. The optimal temperature and pH of rLAP II were 55 °C and 8.0 in phosphate buffer, and its activity was strongly stimulated by Co(2+). Conformational studies indicated that maintaining the α-helical structure had a critical effect on rLAP II activity. rLAP II was used to hydrolyze anchovy proteins, and it exhibited high specificity for peptides with molecular weight between 6000 and 1000 Da and positive coordination with endogenous enzymes and commercial Flavourzyme. Its use will enhance protein hydrolysis in species of aquatic animals. rLAP II could potentially be used to remove bitterness in the protein hydrolysis industry.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, Guangdong 510640, PR China.
authors.forename: Fanghua
authors.initials: F
authors.lastname: Wang
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ning
authors.forename: Dongming
authors.initials: D
authors.lastname: Lan
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D029941:Fish Proteins / Q000737:chemistry*; D005399:Fishes; D001411:Geobacillus stearothermophilus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D029941:Fish Proteins; D011994:Recombinant Proteins; D007931:Leucyl Aminopeptidase