title: A hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin in palladium nanoparticles/graphene-chitosan nanocomposite film.
abstract: Thermally two-dimensional lattice graphene (GR) and biocompatibility chitosan (CS) act as a suitable support for the deposition of palladium nanoparticles (PdNPs). A novel hydrogen peroxide (H(2)O(2)) biosensor based on immobilization of hemoglobin (Hb) in thin film of CS containing GR and PdNPs was developed. The surface morphologies of a set of representative membranes were characterized by means of scanning electron microscopy and showed that the PdNPs are of a sphere shape and an average diameter of 50 nm. Under the optimal conditions, the immobilized Hb showed fast and excellent electrocatalytic activity to H(2)O(2) with a small Michaelis-Menten constant of 16 μmol L(-1), a linear range from 2.0 × 10(-6) to 1.1 × 10(-3) mol L(-1), and a detection limit of 6.6 × 10(-7) mol L(-1). The biosensor also exhibited other advantages, good reproducibility, and long-term stability, and PdNPs/GR-CS nanocomposites film would be a promising material in the preparation of third generation biosensor.
authors.affiliation: Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, People's Republic of China.
authors.forename: Aili
authors.initials: A
authors.lastname: Sun
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Sheng
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D010165:Palladium; D010316:Particle Size; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins; D010165:Palladium; D006108:Graphite; D048271:Chitosan; D006861:Hydrogen Peroxide