title: Interaction between serotoninergic-and β-adrenergic receptors signaling pathways in rat femoral artery.
abstract: BACKGROUND
  Coronary heart disease has been widely studied in cardiovascular research. However, patients with peripheral artery disease (PAD) have worst outcomes compared to those with coronary artery disease. Therefore, pharmacological studies using femoral artery are highly relevant for a better understanding of the pathophysiologic responses of the PAD.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the pharmacologic properties of the contractile and relaxing agents in rat femoral artery.
  
  
  METHODS
  Concentration response curves to the contractile phenylephrine (PE) and serotonin (5-HT) and the relaxing agents isoproterenol (ISO) and forskolin were obtained in isolated rat femoral artery. For relaxing responses, tissues were precontracted with PE or 5-HT.
  
  
  RESULTS
  The order rank potency in femoral artery was 5-HT > PE for contractile responses. In tissues precontracted with 5-HT, relaxing responses to isoproterenol was virtually abolished as compared to PE-contracted tissues. Forskolin, a stimulant of adenylyl cyclase, partially restored the relaxing response to ISO in 5-HT-precontracted tissues.
  
  
  CONCLUSIONS
  An interaction between β-adrenergic- and serotoninergic- receptors signaling pathway occurs in femoral artery. Moreover, this study provides a new model to study serotoninergic signaling pathway under normal and pathological conditions which can help understanding clinical outcomes in the PAD.
authors.affiliation: Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil. nadelbin@hotmail.com
authors.forename: Maria Andréia
authors.initials: MA
authors.lastname: Delbin
authors.forename: Alexandre Sérgio
authors.initials: AS
authors.lastname: Silva
authors.forename: Edson
authors.initials: E
authors.lastname: Antunes
authors.forename: Angelina
authors.initials: A
authors.lastname: Zanesco
mesh_terms: D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D005263:Femoral Artery / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D023421:Models, Animal; D058729:Peripheral Arterial Disease / Q000503:physiopathology*; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D011985:Receptors, Serotonin / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D011943:Receptors, Adrenergic, beta; D011985:Receptors, Serotonin; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D005576:Colforsin; D010656:Phenylephrine; D012701:Serotonin; D007545:Isoproterenol