title: Selective protein separation using siliceous materials with a trimethoxysilane-containing glycopolymer.
abstract: A copolymer with α-D-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)-immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.
authors.affiliation: Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Seto
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Ogata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
authors.forename: Yu
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012821:Silanes / Q000737:chemistry*
chemical_list: D011108:Polymers; D011506:Proteins; D012821:Silanes; D008358:Mannose; C071717:trimethoxysilane