title: 16S partial gene mitochondrial DNA and internal transcribed spacers ribosomal DNA as differential markers of Trichuris discolor populations.
abstract: Comparative morphological, biometrical and molecular studies of Trichuris discolor isolated from Bos taurus from Spain and Iran was carried out. Furthermore, Trichuris ovis isolated from B. taurus and Capra hircus from Spain has been, molecularly, analyzed. Morphological studies revealed clear differences between T. ovis and T. discolor isolated from B. taurus but differences were not observed between populations of T. discolor isolated from different geographical regions. Nevertheless, the molecular studies based on the amplification and sequencing of the internal transcribed spacers 1 and 2 ribosomal DNA and 16S partial gene mitochondrial DNA showed clear differences between both populations of T. discolor from Spain and Iran suggesting two cryptic species. Phylogenetic studies corroborated these data. Thus, phylogenetic trees based on ITS1, ITS2 and 16S partial gene sequences showed that individuals of T. discolor from B. taurus from Iran clustered together and separated, with high bootstrap values, of T. discolor isolated from B. taurus from Spain, while populations of T. ovis from B. taurus and C. hircus from Spain clustered together but separated with high bootstrap values of both populations of T. discolor. Furthermore, a comparative phylogenetic study has been carried out with the ITS1and ITS2 sequences of Trichuris species from different hosts. Three clades were observed: the first clustered all the species of Trichuris parasitizing herbivores (T. discolor, T. ovis, Trichuris leporis and Trichuris skrjabini), the second clustered all the species of Trichuris parasitizing omnivores (Trichuris trichiura and Trichuris suis) and finally, the third clustered species of Trichuris parasitizing carnivores (Trichuris muris, Trichuris arvicolae and Trichuris vulpis).
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Prof. García González 2, 41012 Seville, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Callejón
authors.forename: A
authors.initials: A
authors.lastname: Halajian
authors.forename: M
authors.initials: M
authors.lastname: de Rojas
authors.forename: A
authors.initials: A
authors.lastname: Marrugal
authors.forename: D
authors.initials: D
authors.lastname: Guevara
authors.forename: C
authors.initials: C
authors.lastname: Cutillas
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003710:Demography; D005260:Female; D005819:Genetic Markers; D007492:Iran / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013030:Spain / Q000453:epidemiology; D014257:Trichuriasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D014258:Trichuris / Q000235:genetics* / Q000502:physiology
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D012336:RNA, Ribosomal, 16S