title: NMR studies of protein-RNA interactions.
abstract: This chapter describes the preparation of NMR quantities of RNA purified to single-nucleotide resolution for protein-RNA interaction studies. The protocol is easily modified to make nucleotide-specific isotopically labeled RNAs or uniformly labeled RNA fragments for ligation to generate segmentally labeled RNAs.
authors.affiliation: Department of Chemistry, University at Albany SUNY, Albany, NY, USA. ctheimer@albany.edu
authors.forename: Carla A
authors.initials: CA
authors.lastname: Theimer
authors.forename: Nakesha L
authors.initials: NL
authors.lastname: Smith
authors.forename: May
authors.initials: M
authors.lastname: Khanna
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D007553:Isotope Labeling / Q000379:methods; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D058727:RNA, Small Untranslated / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D011506:Proteins; D058727:RNA, Small Untranslated