title: Crystallization and preliminary X-ray crystallographic studies of VibE, a vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase from Vibrio cholerae.
abstract: Vibriobactin synthetases (VibABCDEFH) catalyze the biosynthesis of vibriobactin in the pathogenic bacterium Vibrio cholerae. VibE, a vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase, plays a critical role in the transfer of 2,3-dihydroxybenzoate to the aryl carrier protein domain of holo VibB. Here, the cloning, protein expression and purification, crystallization and preliminary X-ray crystallographic analysis of VibE from V. cholerae are reported. The VibE crystal diffracted to 2.3 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 56.471, b = 45.927, c = 77.014 Å, β = 95.895°. There is one protein molecule in the asymmetric unit, with a corresponding Matthews coefficient of 1.63 Å(3) Da(-1) and solvent content of 24.41%.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Liu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Deyu
authors.initials: D
authors.lastname: Zhu
authors.forename: Tiandi
authors.initials: T
authors.lastname: Wei
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
authors.forename: Sujuan
authors.initials: S
authors.lastname: Xu
mesh_terms: D001430:Bacteriocins / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D001430:Bacteriocins; C053207:vibriocin; D008049:Lipase