title: Detection and characterisation of papillomavirus in skin lesions of giraffe and sable antelope in South Africa.
abstract: Papillomavirus was detected electron microscopically in cutaneous fibropapillomas of a giraffe (Giraffa camelopardalis) and a sable antelope (Hippotragus niger). The virus particles measured 45 nm in diameter. Histopathologically, the lesions showed histopathological features similar to those of equine sarcoid as well as positive immunoperoxidase-staining of tissue sections for papillomavirus antigen. Polymerase chain reaction (PCR) detected bovine papillomavirus (BPV) DNA. Bovine papillomavirus-1 was characterised by real-time PCR in the sable and giraffe, and cloning and sequencing of the PCR product revealed a similarity to BPV-1. As in the 1st giraffe, the lesions from a 2nd giraffe revealed locally malignant pleomorphism, possibly indicating the lesional end-point of papilloma infection. Neither virus particles nor positively staining papillomavirus antigen could be demonstrated in the 2nd giraffe but papillomavirus DNA was detected by real-time PCR which corresponded with BPV-1 and BPV-2.
authors.affiliation: Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. enettevd@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: van Dyk
authors.forename: A M
authors.initials: AM
authors.lastname: Bosman
authors.forename: E
authors.initials: E
authors.lastname: van Wilpe
authors.forename: J H
authors.initials: JH
authors.lastname: Williams
authors.forename: R G
authors.initials: RG
authors.lastname: Bengis
authors.forename: J
authors.initials: J
authors.lastname: van Heerden
authors.forename: E H
authors.initials: EH
authors.lastname: Venter
mesh_terms: D000818:Animals; D000866:Antelopes / Q000821:virology*; D001188:Artiodactyla / Q000821:virology*; D052688:Bovine papillomavirus 1 / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005350:Fibroma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D008297:Male; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012878:Skin Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary; D013019:South Africa
chemical_list: D004279:DNA, Viral