title: Phenotypic characterization and random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida isolated from Korean pigs.
abstract: Pasteurella multocida causes various respiratory disease symptoms in pigs, including atrophic rhinitis and pneumonia. In the present study, 69 strains of P. multocida were isolated from 443 pigs with respiratory clinical symptoms at 182 farms located throughout South Korea from 2009 to 2010. A multiplex capsular PCR typing assay revealed that 69 strains of P. multocida isolated in this study had the biosynthetic locus of the capsules of either serogroup A (47 strains, 68.1%) or serogroup D (22 strains, 31.9%). The 22 strains positive for serogroup D-specific primers were divided into four clusters and the 47 strains positive for serogroup A-specific primers were divided into 12 clusters according to the results of Random Amplified Polymorphic DNA (RAPD) analysis. P. multocida strains in the present study were susceptible to most of the antimicrobial agents used. An analysis of antimicrobial resistance and virulence gene pattern combined with RAPD indicated that a certain P. multocida strain appeared to be genetically identical, implying the persistence of the strain within a single farm.
authors.affiliation: Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, 430-824, South Korea.
authors.forename: Ki-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Jeoung
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Lee
authors.forename: Myoung-Heon
authors.initials: MH
authors.lastname: Lee
authors.forename: Hwan-Won
authors.initials: HW
authors.lastname: Choi
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Chang
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Oh
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: An
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005838:Genotype; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D056910:Republic of Korea / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents