title: Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM.
abstract: The flax rust effector AvrM is a secreted protein of unknown fold that is recognized by the M resistance protein in flax. In order to investigate the structural basis of the AvrM-M interaction and possible virulence-associated functions of AvrM, the C-terminal domains of two different AvrM variants (AvrM-A and avrM) were crystallized. Crystals of native AvrM-A were obtained using pentaerythritol ethoxylate (15/4 EO/OH) as a precipitant and diffracted X-rays to 2.9 Å resolution. Selenomethionine-derivative crystals of similar quality were obtained using PEG 1500 as a precipitant. Both the native and selenomethionine-labelled AvrM-A crystals had symmetry of space group C222(1) with eight molecules in the asymmetric unit. Crystals of avrM had symmetry of space group P2(1)2(1)2(1) and diffracted X-rays to 2.7 Å resolution. Initial AvrM-A phases were calculated using the single-wavelength anomalous dispersion (SAD) method and a partial model was built. Phases for avrM were obtained by molecular replacement using the partial AvrM-A model.
authors.affiliation: School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology), University of Queensland, Brisbane, Queensland, Australia. t.ve@uq.edu.au
authors.forename: Thomas
authors.initials: T
authors.lastname: Ve
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Williams
authors.forename: Anna
authors.initials: A
authors.lastname: Stamp
authors.forename: Eugene
authors.initials: E
authors.lastname: Valkov
authors.forename: Peter N
authors.initials: PN
authors.lastname: Dodds
authors.forename: Peter A
authors.initials: PA
authors.lastname: Anderson
authors.forename: Bostjan
authors.initials: B
authors.lastname: Kobe
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D019597:Flax / Q000737:chemistry*; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D010940:Plant Proteins