title: Simple chemiluminescence aptasensors based on resonance energy transfer.
abstract: In the present work, two aptamer-based probes and related sensor systems were developed with chemiluminescence signaling.  The detection was based on "turning-on" chemiluminescence with switching "off" of the resonance energy transfer after the aptamer's recognition of the target molecule.  In this design, a DNA/aptamer duplex linked a chemiluminescence group and a gold nanoparticle together.  Only low-intensity chemiluminescence was obtained due to the highly efficient resonance energy transfer.  After introducting the target molecule, structure-switching took place with turning off the energy transfer; thus, a restoration and turning on of the chemiluminescence was obtained.  The two designs differed in the chemiluminescence groups, since one was a covalently linked luminol molecule, while the other was a conjugated horseradish peroxidase for the catalysis of further chemiluminescence reactions.  These schemes provided simple and effective sensing toward a model analyte, adenosine.
authors.affiliation: Department of Chemistry, Suzhou University, 199 Ren’ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Yeqian
authors.initials: Y
authors.lastname: Jin
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shi
authors.forename: Mengyun
authors.initials: M
authors.lastname: Zhou
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Tu
authors.forename: Jilin
authors.initials: J
authors.lastname: Yan
mesh_terms: D052157:Aptamers, Nucleotide; D001483:Base Sequence; D015374:Biosensing Techniques; D002384:Catalysis; D017931:DNA Primers; D004735:Energy Transfer; D057230:Limit of Detection; D049449:Luminescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D052157:Aptamers, Nucleotide; D017931:DNA Primers