title: Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain.
abstract: L. acidophilus La-14 produces bacteriocin active against L. monocytogenes ScottA (1600 AU/ml) in MRS broth at 30°C or 37°C. The bacteriocin proved inhibitory to different serological types of Listeria spp. Antimicrobial activity was completely lost after treatment of the cell-free supernatant with proteolytic enzymes. Addition of bacteriocin produced by L. acidophilus La-14 to a 3 h-old culture of L. monocytogenes ScottA repressed cell growth in the following 8h. Treatment of stationary phase cells of L. monocytogenes ScottA (107-108 CFU/ml) by the bacteriocin resulted in growth inhibition. Growth of L. acidophilus La-14 was not inhibited by commercial drugs from different generic groups, including nonsteroidal anti-inflammatory drugs (NSAID) containing diclofenac potassium or ibuprofen arginine. Only one non-antibiotic drug tested, Atlansil (an antiarrhythmic agent), had an inhibitory effect on L. acidophilus La-14 with MIC of 2.5 mg/ml. L. acidophilus La-14 was not affected by drugs containing sodium or potassium diclofenac. L. acidophilus La-14 shows a good resistance to several drugs and may be applied in combination for therapeutic use.
authors.affiliation: Universidade de Sao Paulo, Departamento de Alimentos e Nutriçao Experimental, Sao Paulo, Brasil. slavi310570@abv.bg
authors.forename: Svetoslav Dimitrov
authors.initials: SD
authors.lastname: Todorov
authors.forename: Danielle Nader
authors.initials: DN
authors.lastname: Furtado
authors.forename: Susana Marta Isay
authors.initials: SM
authors.lastname: Saad
authors.forename: Bernadette Dora
authors.initials: BD
authors.lastname: Gombossy de Melo Franco
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D004798:Enzymes / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008828:Microbiological Techniques; D019936:Probiotics / Q000378:metabolism* / Q000627:therapeutic use; D053038:Quorum Sensing / Q000187:drug effects; D013696:Temperature
chemical_list: D000700:Analgesics; D000889:Anti-Arrhythmia Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001430:Bacteriocins; D003902:Detergents; D004798:Enzymes