title: Influence of erythrocyte oxygenation and intravascular ATP on resting and exercising skeletal muscle blood flow in humans with mitochondrial myopathy.
abstract: Oxygen (O₂) extraction is impaired in exercising skeletal muscle of humans with mutations of mitochondrial DNA (mtDNA), but the muscle hemodynamic response to exercise has never been directly investigated. This study sought to examine the extent to which human skeletal muscle perfusion can increase without reductions in blood oxygenation and to determine whether erythrocyte O₂ off-loading and related ATP vascular mechanisms are impaired in humans with mutations of mtDNA. Leg vascular hemodynamic, oxygenation and ATP were investigated in ten patients with mtDNA mutations and ten matched healthy control subjects: 1) at rest during normoxia, hypoxia, hyperoxia and intra-femoral artery ATP infusion, and 2) during passive and dynamic one-legged knee-extensor exercises. At rest, blood flow (LBF), femoral arterial and venous blood oxygenation and plasma ATP were similar in the two groups. During dynamic exercise, LBF and vascular conductance increased 9-10 fold in the patients despite erythrocyte oxygenation and leg O₂ extraction remained unchanged (p<0.01). In the patients, workload-adjusted LBF was 28% to 62% higher during submaximal- and maximal exercises and was associated with augmented plasma ATP. The appropriate hemodynamic adjustments during severe hypoxia and ATP infusion suggest that erythrocyte O₂ off-loading and related ATP vascular mechanisms are intact in patients with mtDNA mutations. Furthermore, greater increase in plasma ATP and LBF at a given metabolic demand in the patients, in concert with unchanged oxyhemoglobin, suggest that erythrocyte O₂ off-loading is not obligatory for the exercise-induced increase in blood flow and intravascular ATP concentration.
authors.affiliation: Department of Neurology, the Neuromuscular Research Unit, Denmark. tina@dysgaard.dk
authors.forename: Tina D
authors.initials: TD
authors.lastname: Jeppesen
authors.forename: John
authors.initials: J
authors.lastname: Vissing
authors.forename: José
authors.initials: J
authors.lastname: González-Alonso
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D000328:Adult; D001783:Blood Flow Velocity; D004272:DNA, Mitochondrial / Q000235:genetics; D004912:Erythrocytes / Q000378:metabolism* / Q000502:physiology; D015444:Exercise / Q000502:physiology; D005260:Female; D006439:Hemodynamics; D006801:Humans; D008297:Male; D008875:Middle Aged; D017240:Mitochondrial Myopathies / Q000378:metabolism / Q000503:physiopathology*; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism / Q000502:physiology*; D010101:Oxygen Consumption; D017354:Point Mutation; D012146:Rest / Q000502:physiology
chemical_list: D004272:DNA, Mitochondrial; D000255:Adenosine Triphosphate