title: A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.
abstract: This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatography. The yield scFv protein reached a purity over 95% after purification. Immunoreactivity assay demonstrated that scFv possessed a special affinity toward CTGF. RT-PCR, western blot, and immunofluorescence experiments showed that increased expression of α-smooth muscle actin induced by TGF-β1 could be suppressed by this scFv antibody through inhibiting the phosphorylation of Akt.
authors.affiliation: Center of Clinical Laboratory Medicine of Zhongda Hospital, Southeast University, Nanjing, China. nationball@163.com
authors.forename: Guoqiu
authors.initials: G
authors.lastname: Wu
authors.forename: Xiyong
authors.initials: X
authors.lastname: Wang
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Deng
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Wu
authors.forename: Xiulei
authors.initials: X
authors.lastname: Xue
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Yan
authors.forename: Xihua
authors.initials: X
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D058628:Myofibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000199:Actins; D057127:Single-Chain Antibodies; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor