title: Development of immortalized hepatocyte-like cells from hMSCs.
abstract: Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies.
authors.affiliation: Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Adisak
authors.initials: A
authors.lastname: Wongkajornsilp
authors.forename: Khanit
authors.initials: K
authors.lastname: Sa-Ngiamsuntorn
authors.forename: Suradej
authors.initials: S
authors.lastname: Hongeng
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D048430:Cell Shape / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction / Q000502:physiology; D005455:Fluorescent Antibody Technique; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: C069077:BMI1 protein, human; D007527:Isoenzymes; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D003577:Cytochrome P-450 Enzyme System; D063150:Polycomb Repressive Complex 1; C509186:TERT protein, human; D019098:Telomerase