title: Early and late calcium waves during wound healing in corneal endothelial cells.
abstract: Immediately after wounding, bovine corneal endothelial cells develop a fast calcium wave that propagates from the wound border to the rest of the monolayer and extinguishes in approximately 5 minutes. One hour after wounding, a late, slow calcium wave (SCW) develops concomitantly to the depolarization of the plasma membrane potential of the border cells. The incorporation of inhibitors of the epithelial sodium channel and of the sodium-calcium exchanger produces inhibition of the membrane depolarization and the SCW, and diminishes the rate of wound healing. The L-type calcium channel blocker nimodipine does not have any effect on the SCW. The reversible inhibition of the fast calcium wave does not affect the SCW and only slightly decreases the velocity of healing. Our results suggest that the SCW is at least partially produced by the coupling of the epithelial sodium channel and the sodium-calcium exchanger functioning in reverse mode. They also suggest that the SCW may play a role in the overall healing process.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. schiffle@mednet.org.uy
authors.forename: Silvia
authors.initials: S
authors.lastname: Chifflet
authors.forename: Cristian
authors.initials: C
authors.lastname: Justet
authors.forename: Julio A
authors.initials: JA
authors.lastname: Hernández
authors.forename: Verónica
authors.initials: V
authors.lastname: Nin
authors.forename: Carlos
authors.initials: C
authors.lastname: Escande
authors.forename: Juan C
authors.initials: JC
authors.lastname: Benech
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000494:pharmacology*; D020013:Calcium Signaling; D002417:Cattle; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology*; D016228:Endothelial Growth Factors; D014945:Wound Healing
chemical_list: D020746:Calcium Channels, L-Type; D016228:Endothelial Growth Factors