title: Stem-loop RT-qPCR for microRNA expression profiling.
abstract: Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan(®) miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR. Increased experimental throughput permits the expression screening of larger number of miRNAs with small amounts of sample. Here, we demonstrate the use of both TaqMan(®) Array Card and OpenArray(®) platforms to accurately determine the level of miRNA gene expression in biological samples.
authors.affiliation: Life Technologies Corporation, Foster City, CA, USA.
authors.forename: James
authors.initials: J
authors.lastname: Hurley
authors.forename: Doug
authors.initials: D
authors.lastname: Roberts
authors.forename: Andrew
authors.initials: A
authors.lastname: Bond
authors.forename: David
authors.initials: D
authors.lastname: Keys
authors.forename: Caifu
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D017931:DNA Primers; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D048348:Reverse Transcription
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D018749:RNA, Plant