title: SigB-dependent tolerance to protein synthesis-inhibiting antibiotics in Listeria monocytogenes EGDe.
abstract: The alternative sigma factor SigB in food-borne pathogen Listeria monocytogenes was determined in this study to be required for tolerance to protein synthesis-inhibiting antibiotics. The minimum inhibitory concentrations of tetracycline HCl and gentamicin sulphate against EGDeΔsigB were two- and fourfold less than those for EGDe, respectively. The ability of EGDeΔsigB to overcome the growth arrest caused by erythromycin and rifampin was also weaker than that of EGDe. The transcription analysis of four genetic loci (known to be induced by rifampin in Bacillus subtili) kat, fri, ropB and rsbU in EGDe and EGDeΔsigB in the absence or presence of rifampin revealed that: (1) expression of kat and fri genes is σ                         (B) dependent, but only the former is inducible by rifampin stress; (2) the transcriptional level of rpoB gene was stable under all the experimental conditions, while that of rsbU in EGDeΔsigB was remarkably higher in the absence of rifampin and significantly increased in EGDe but reduced in EGDeΔsigB after rifampin application, when compared to those in EGDe and EGDeΔsigB control without antibiotic, respectively. These results suggest that complex physiological reactions to tolerance of the antibiotic stress are variably regulated in bacteria, and in contrast to B. subtilis, rsbU in EGDeΔsigB may compensate for the σ                         (B)-dependent genes that are necessary for tolerance to rifampin stress and therefore plays a role in overcoming the antibiotic-triggered growth arrest.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Luo Yu Road 152, Wuhan, People's Republic of China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Yin
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Feng
authors.forename: Junli
authors.initials: J
authors.lastname: Shang
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004361:Drug Tolerance; D004917:Erythromycin / Q000494:pharmacology; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D014176:Protein Biosynthesis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; C480945:SigB protein, Listeria monocytogenes; D012808:Sigma Factor; D004917:Erythromycin; D013752:Tetracycline; D012293:Rifampin