title: [Effects of Mycobacterium tuberculosis Hsp16.3 protein on the autophagy function of mice macrophages].
abstract: OBJECTIVE
  To investisate  the inhibition of Hsp-16.3 on the autophagosomes formation of macrophages.
  
  
  METHODS
  Mouse  RAW264.7 macrophages   were   induced by rapamycin (50 ng/μL) following infection with M.tuberculosis H37Rv strains,  thereafter,  co-incubated with Hsp16.3 protein (25 μg/mL). The effects of Hsp16.3 protein on the autophagosomes formation was observed with   transmission electron microscope. The expression of autophagy-related genes (atg8) for macrophages was detected by Western blotting.
  
  
  RESULTS
  It   was found that rapamycin-induced autophagy of macrophages infected with M.tuberculosis H37Rv enhanced localization of mycobacteria with autophagosomes. Hsp16.3 protein inhibits autophagosome formation and affects M.tuberculosis survival inside infected macrophages. Furthermore,  Hsp16.3 protein significantly increased M.tuberculosis colony forming units (CFU),  and decreased the expression of microtubule-associated protein light chain-3 (LC3) expression level  (P<0.05).
  
  
  CONCLUSIONS
  The  results showed  that Hsp16.3 protein inhibits the formation of autophagosomes by regulating the expression  of LC3 protein.
authors.affiliation: Laboratory Animals Center,  Fourth Military Medical University,  Xi'an 710032,  China.
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Shi
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Feng-feng
authors.initials: FF
authors.lastname: Mao
authors.forename: Cai-qin
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Hai
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology*; D018833:Chaperonins / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis; D010588:Phagosomes / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; C431133:Hsp16.3 protein, Mycobacterium tuberculosis; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D018833:Chaperonins