title: Proteomic analysis of microvesicles derived from human mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) have emerged as a promising means for treating degenerative or incurable diseases. Recent studies have shown that microvesicles (MVs) from MSCs (MSC-MVs) contribute to recovery of damaged tissues in animal disease models. Here, we profiled the MSC-MV proteome to investigate their therapeutic effects. LC-MS/MS analysis of MSC-MVs identified 730 MV proteins. The MSC-MV proteome included five positive and two variable known markers of MSCs, but no negative marker, as well as 43 surface receptors and signaling molecules controlling self-renewal and differentiation of MSCs. Functional enrichment analysis showed that cellular processes represented by the MSC-MV proteins include cell proliferation, adhesion, migration, and morphogenesis. Integration of MSC's self-renewal and differentiation-related genes and the proteome of MSC-conditioned media (MSC-CM) with the MSC-MV proteome revealed potential MV protein candidates that can be associated with the therapeutic effects of MSC-MVs: (1) surface receptors (PDGFRB, EGFR, and PLAUR); (2) signaling molecules (RRAS/NRAS, MAPK1, GNA13/GNG12, CDC42, and VAV2); (3) cell adhesion (FN1, EZR, IQGAP1, CD47, integrins, and LGALS1/LGALS3); and (4) MSC-associated antigens (CD9, CD63, CD81, CD109, CD151, CD248, and CD276). Therefore, the MSC-MV proteome provides a comprehensive basis for understanding the potential of MSC-MVs to affect tissue repair and regeneration.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine , 50 Yonsei-ro, Seodaemoon-gu, Seoul 120-752, Republic of Korea.
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.forename: So Jeong
authors.initials: SJ
authors.lastname: Yun
authors.forename: Seong-Mi
authors.initials: SM
authors.lastname: Choi
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jung
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002478:Cells, Cultured; D022162:Cytoplasmic Vesicles / Q000737:chemistry* / Q000378:metabolism; D055808:Drug Discovery; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D011506:Proteins; D020543:Proteome