title: Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the periplasmic domain of outer membrane protein A from Acinetobacter baumannii.
abstract: Outer membrane protein A from Acinetobacter baumannii (AbOmpA) is a major outer membrane protein and a key player in the bacterial pathogenesis that induces host cell death. AbOmpA is presumed to consist of an N-terminal β-barrel transmembrane domain and a C-terminal periplasmic OmpA-like domain. In this study, the recombinant C-terminal periplasmic domain of AbOmpA was overexpressed in Escherichia coli, purified and crystallized using the vapour-diffusion method. A native diffraction data set was collected to a resolution of 2.0 Å using synchrotron radiation. The space group of the crystal was P2(1), with unit-cell parameters a = 58.24, b = 98.59, c = 97.96 Å, β = 105.92°. The native crystal contained seven or eight molecules per asymmetric unit and had a calculated Matthews coefficient of 2.93 or 2.56 Å(3) Da(-1).
authors.affiliation: Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chungbuk, Republic of Korea.
authors.forename: Jeong Soon
authors.initials: JS
authors.lastname: Park
authors.forename: Woo Cheol
authors.initials: WC
authors.lastname: Lee
authors.forename: Saehae
authors.initials: S
authors.lastname: Choi
authors.forename: Kwon Joo
authors.initials: KJ
authors.lastname: Yeo
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Song
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Han
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Jeon
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Kim
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins