title: [Western blot as a confirming test in the laboratory diagnosis of syphilis].
abstract: Two hundred and ninety-five patients who had been found to have Treponema pallidum antibodies detected by enzyme immunoassay were additionally studied by a Western blot test to confirm their presence. Every four cases were ascertained to be false-positive, false seropositivity being more frequent in the presence of IgM antibody against T. palladium. Spinal fluid analysis provided evidence for the course of neurosyphilis in 5 cases. The diagnosis of congenital syphilis was verified in 2 children who had p15, p17, p45, and 47. The findings demonstrate it necessary to extensively use a Western blot in the health care system.
authors.forename: A I
authors.initials: AI
authors.lastname: Novikov
authors.forename: T I
authors.initials: TI
authors.lastname: Dolgikh
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Novikov
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D015153:Blotting, Western / Q000379:methods*; D057069:Diagnostic Test Approval; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009494:Neurosyphilis / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000276:immunology; D011247:Pregnancy; D013588:Syphilis Serodiagnosis / Q000379:methods*; D013590:Syphilis, Congenital / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D014210:Treponema pallidum / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M