title: Arabidopsis EDS1 connects pathogen effector recognition to cell compartment-specific immune responses.
abstract: Pathogen effectors are intercepted by plant intracellular nucleotide binding-leucine-rich repeat (NB-LRR) receptors. However, processes linking receptor activation to downstream defenses remain obscure. Nucleo-cytoplasmic basal resistance regulator EDS1 (ENHANCED DISEASE SUSCEPTIBILITY1) is indispensible for immunity mediated by TIR (Toll-interleukin-1 receptor)-NB-LRR receptors. We show that Arabidopsis EDS1 molecularly connects TIR-NB-LRR disease resistance protein RPS4 recognition of bacterial effector AvrRps4 to defense pathways. RPS4-EDS1 and AvrRps4-EDS1 complexes are detected inside nuclei of living tobacco cells after transient coexpression and in Arabidopsis soluble leaf extracts after resistance activation. Forced AvrRps4 localization to the host cytoplasm or nucleus reveals cell compartment-specific RPS4-EDS1 defense branches. Although nuclear processes restrict bacterial growth, programmed cell death and transcriptional resistance reinforcement require nucleo-cytoplasmic coordination. Thus, EDS1 behaves as an effector target and activated TIR-NB-LRR signal transducer for defenses across cell compartments.
authors.affiliation: Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions, D-50829 Cologne, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Heidrich
authors.forename: Lennart
authors.initials: L
authors.lastname: Wirthmueller
authors.forename: Céline
authors.initials: C
authors.lastname: Tasset
authors.forename: Cécile
authors.initials: C
authors.lastname: Pouzet
authors.forename: Laurent
authors.initials: L
authors.lastname: Deslandes
authors.forename: Jane E
authors.initials: JE
authors.lastname: Parker
mesh_terms: D017209:Apoptosis; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007113:Immunity, Innate; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C098294:AvrRps4 protein, Pseudomonas syringae; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C097421:rps4 protein, plant