title: Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging.
abstract: We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.
authors.forename: Metasebya
authors.initials: M
authors.lastname: Solomon
authors.forename: Brian R
authors.initials: BR
authors.lastname: White
authors.forename: Ralph E
authors.initials: RE
authors.lastname: Nothdruft
authors.forename: Walter
authors.initials: W
authors.lastname: Akers
authors.forename: Gail
authors.initials: G
authors.lastname: Sudlow
authors.forename: Adam T
authors.initials: AT
authors.lastname: Eggebrecht
authors.forename: Samuel
authors.initials: S
authors.lastname: Achilefu
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Culver