title: Purification, crystallization and preliminary crystallographic analysis of peroxidase from the palm tree Chamaerops excelsa.
abstract: Plant peroxidases are presently used extensively in a wide range of biotechnological applications owing to their high environmental and thermal stability. As part of efforts towards the discovery of appealing new biotechnological enzymes, the peroxidase from leaves of the palm tree Chamaerops excelsa (CEP) was extracted, purified and crystallized in its native form. An X-ray diffraction data set was collected at a synchrotron source and data analysis showed that the CEP crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 70.2, b = 100.7, c = 132.3 Å.
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos-SP, Brazil.
authors.forename: Larissa C
authors.initials: LC
authors.lastname: Textor
authors.forename: Jademilson C
authors.initials: JC
authors.lastname: Santos
authors.forename: Nazaret Hidalgo
authors.initials: NH
authors.lastname: Cuadrado
authors.forename: Manuel G
authors.initials: MG
authors.lastname: Roig
authors.forename: Galina G
authors.initials: GG
authors.lastname: Zhadan
authors.forename: Valery L
authors.initials: VL
authors.lastname: Shnyrov
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D028023:Arecaceae / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D010544:Peroxidases