title: Method for the rapid determination of protein in meats using the CEM Sprint protein analyzer: collaborative study.
abstract: A collaborative study was conducted to determine the protein content of raw and processed meat products by a protein-tagging and colorimetric technique. Meat products were prepared following AOAC Official Method 983.18 and analyzed using CEM Corporation's Sprint Rapid Protein Analyzer. Sprint provides protein results by combining an accurately weighed test portion with a known amount of dye-binding agent. The dye-binding agent binds with the lysine, histidine, and arginine, as well as the n-terminus of the proteins commonly found in raw meat and processed meat products. Results are displayed and reported by the Sprint as a percentage (g/100 g) of protein. Ten blind duplicate study samples were sent to 10 collaborating laboratories in the United States. The within-laboratory (repeatability) relative standard deviation (RSD(r)) ranged from 0.91 to 3.04%, and between-laboratories (reproducibility) relative standard deviation (RSDR) ranged from 1.50 to 3.41% for protein. The method is recommended for Official First Action.
authors.affiliation: CEM Corporation, 3100 Smith Farm Rd, Matthews, NC 28105, USA. cindy.moser@cem.com
authors.forename: Cindy
authors.initials: C
authors.lastname: Moser
authors.forename: Kathy
authors.initials: K
authors.lastname: Herman
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D002645:Chickens; D004044:Dietary Proteins / Q000032:analysis*; D004285:Dogs; D007753:Laboratories; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013552:Swine; D014422:Turkeys
chemical_list: D004044:Dietary Proteins