title: Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells.
abstract: The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described.
authors.affiliation: Developmental Biology Unit, UCL Institute of Child Health, University College London, London, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Gualdoni
authors.forename: Michael
authors.initials: M
authors.lastname: Baron
authors.forename: Jörn
authors.initials: J
authors.lastname: Lakowski
authors.forename: Sarah
authors.initials: S
authors.lastname: Decembrini
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Pearson
authors.forename: Robin R
authors.initials: RR
authors.lastname: Ali
authors.forename: Jane C
authors.initials: JC
authors.lastname: Sowden
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002924:Ciliary Body / Q000166:cytology*; D004622:Embryo, Mammalian / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D012160:Retina / Q000166:cytology*; D018874:Spheroids, Cellular; D013234:Stem Cells / Q000166:cytology*; D019076:Transgenes / Q000235:genetics