title: Validation of a polymerase chain reaction-oligochromatography test for detection of influenza A (H1N1) 2009 virus.
abstract: The outbreak of pandemic influenza A (H1N1) 2009 virus caused the first influenza pandemic disease of the 21st century. In August 2010, the pandemic moved into the post-pandemic period. However, localized outbreaks of various magnitudes continued with a higher rate of disease severity. The aim of this study was to assess a new polymerase chain reaction (PCR)-oligochromatographic assay (Speed-Oligo) in the diagnosis of novel influenza A (H1N1) 2009. A total of 405 nasopharyngeal aspirate specimens from 400 pediatric and adults patients with suspected infection of pandemic influenza A (H1N1) 2009 were analyzed. The sensitivity and specificity values of the Speed-Oligo assay in comparison to reverse transcriptase-PCR assay developed by the Centers for Disease Control and Prevention were 86.5% and 92.2%, respectively. The new assay is simple, rapid, and provides a good sensitivity for detection of influenza A (H1N1) 2009. This assay might be a good alternative to real-time PCR assays for laboratories not equipped with real-time PCR instruments.
authors.affiliation: Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació en Ciènces de la Salut Germans Trias i Pujol, Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08916 Badalona, Spain. loretoffi@gmail.com
authors.forename: Loreto
authors.initials: L
authors.lastname: Fuenzalida
authors.forename: Cristina
authors.initials: C
authors.lastname: Prat
authors.forename: Silvia
authors.initials: S
authors.lastname: Blanco
authors.forename: Albert
authors.initials: A
authors.lastname: Bas
authors.forename: M J
authors.initials: MJ
authors.lastname: Domínguez
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Hernández
authors.forename: Carlos
authors.initials: C
authors.lastname: Rodrigo
authors.forename: Vicente
authors.initials: V
authors.lastname: Ausina
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002845:Chromatography / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D058873:Pandemics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013030:Spain / Q000453:epidemiology; D055815:Young Adult