title: The effect of platelet rich plasma from bone marrow aspirate with added bone morphogenetic protein-2 on the Achilles tendon-bone junction in rabbits.
abstract: BACKGROUND
  To determine if exogenously injected bone marrow derived platelet-rich plasma (PRP) plus bone morphogenetic protein (BMP)-2 could accelerate the healing of bone-tendon junction injuries and increase the junction holding strength during the early regeneration period.
  
  
  METHODS
  A direct injury model of the bone-tendon junction was made using an Achilles tendon-calcaneus bone junction in a rabbit. In the PRP/BMP-2/fibrin group, 0.05 mL of bone marrow derived PRP and 100 ng/mL of BMP-2 both incorporated into 0.1 mL of fibrin glue were injected into Achilles tendon-calcaneus bone junctions. The effect of the intervention was tested by comparing the results of an intervention group to a control group. The results of biomechanical testing, and histological and gross analyses were compared between the 2 groups at the following time points after surgery: 2 weeks, 4 weeks, and 8 weeks.
  
  
  RESULTS
  Histologic examinations showed that woven bone developed in tendon-bone junctions at 2 weeks after surgery in the PRP/BMP-2/fibrin group. Mechanical test results showed no significant difference between the PRP/BMP-2/fibrin and control groups at 2 and 4 weeks after surgery, but the mean maximal load in the PRP/BMP-2/fibrin group was significantly higher than in the control group (p < 0.05) at 8 weeks after surgery.
  
  
  CONCLUSIONS
  Bone marrow derived PRP and BMP-2 in fibrin glue accelerated healing in a rabbit model of tendon-bone junction injury.
authors.affiliation: Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea.
authors.forename: Hak Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyok-Woo
authors.initials: HW
authors.lastname: Nam
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Hur
authors.forename: Misu
authors.initials: M
authors.lastname: Park
authors.forename: Hee Seok
authors.initials: HS
authors.lastname: Yang
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Park
mesh_terms: D000125:Achilles Tendon / Q000293:injuries*; D000818:Animals; D001853:Bone Marrow; D055396:Bone Morphogenetic Protein 2 / Q000627:therapeutic use*; D002111:Calcaneus / Q000293:injuries*; D008297:Male; D053657:Platelet-Rich Plasma; D011817:Rabbits
chemical_list: D055396:Bone Morphogenetic Protein 2