title: Electrical stimulation affects metabolic enzyme phosphorylation, protease activation, and meat tenderization in beef.
abstract: The objective of this study was to investigate the response of sarcoplasmic proteins in bovine LM to low-voltage electrical stimulation (ES; 80 V, 35 s) after dressing and its contribution to meat tenderization at an early postmortem time. Proteome analysis showed that ES resulted in decreased (P < 0.05) phosphorylation of creatine kinase M chain, fructose bisphosphate aldolase C-A, β-enolase, and pyruvate kinase at 3 h postmortem. Zymography indicated an earlier (P < 0.05) activation of μ-calpain in ES muscles. Free lysosomal cathepsin B and L activity increased faster (P < 0.05) in ES muscles up to 24 h. Immunohistochemistry and transmission electron microscopy further indicated that lysosomal enzymes were released at an early postmortem time. Electrical stimulation also induced ultrastructural disruption of sarcomeres. In addition, ES accelerated (P < 0.05) the depletion of ATP, creatine phosphate, and glycogen, as well as a pH decline and the more preferred pH/temperature decline mode. Finally, ES accelerated meat tenderization, resulting in lesser (P < 0.05) shear force values than the control over the testing time. A possible relationship was suggested between a change in the phosphorylation of energy metabolic enzymes and the postmortem tenderization of beef. Our results suggested the possible importance of the activation of μ-calpain, phosphorylation of sarcoplasmic proteins, and release of lysosomal enzymes for ES-induced tenderization of beef muscle.
authors.affiliation: Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. chbli2002@hotmail.com
authors.forename: C B
authors.initials: CB
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: G H
authors.initials: GH
authors.lastname: Zhou
authors.forename: R
authors.initials: R
authors.lastname: Lametsch
authors.forename: P
authors.initials: P
authors.lastname: Ertbjerg
authors.forename: D A
authors.initials: DA
authors.lastname: Brüggemann
authors.forename: H G
authors.initials: HG
authors.lastname: Huang
authors.forename: A H
authors.initials: AH
authors.lastname: Karlsson
authors.forename: M
authors.initials: M
authors.lastname: Hviid
authors.forename: K
authors.initials: K
authors.lastname: Lundström
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004558:Electric Stimulation; D006003:Glycogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D008460:Meat / Q000592:standards*; D018482:Muscle, Skeletal / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D010766:Phosphorylation; D013696:Temperature
chemical_list: D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006003:Glycogen; D010447:Peptide Hydrolases