title: Liquid chromatography-tandem mass spectrometry for the determination of sphingomyelin species from calf brain, ox liver, egg yolk, and krill oil.
abstract: In this study, molecular species of sphingomyelin (SM) in egg yolk, calf brain, ox liver, and krill oil were investigated. Classes of phospholipids (PLs) were purified, identified, and quantified by normal phase semipreparative high-performance liquid chromatography (HPLC) combined with evaporative light scattering detectors (ELSD). For SM molecular species identification, pure SM collected through a flow splitter was loaded to HPLC-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(2)), with 100% methanol containing 5 mM ammonium formate as mobile phase. In addition to classes of PLs, the used approach allowed the determination of profiles of SM species in egg yolk, ox liver, and calf brain, whereas krill oil turned out not to contain any SM. It also allowed the separation and identification of SM subclasses, as well as tentative identification of species with the same molecular mass, including isomers. The results showed that egg yolk contained the highest proportion of (d18:1-16:0)SM (94.1%). The major SM molecular species in ox liver were (d18:1-16:0)SM (25.5%), (d18:1-23:0)SM (19.7%), (d18:1-24:0)SM (13.2%), and (d18:1-22:0)SM (12.5%). Calf brain SM was rich in species such as (d18:1-18:0)SM (40.7%), (d18:1-24:1)SM (17.1%), and (d18:1-20:0)SM (10.8%).
authors.affiliation: Equipe de Chimie Analytique des Molécules Bio-Actives, UMR7178, IPHC-CNRS, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Minjie
authors.initials: M
authors.lastname: Zhao
authors.forename: Saïd
authors.initials: S
authors.lastname: Ennahar
authors.forename: Françoise
authors.initials: F
authors.lastname: Bindler
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004530:Egg Yolk / Q000737:chemistry*; D033201:Euphausiacea / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013109:Sphingomyelins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013109:Sphingomyelins