title: An oral Sindbis virus replicon-based DNA vaccine containing VP2 gene of canine parvovirus delivered by Escherichia coli elicits immune responses in dogs.
abstract: A Sindbis virus replicon-based DNA vaccine containing VP2 gene of canine parvovirus (CPV) was delivered by Escherichia coli to elicit immune responses. The orally immunized dogs developed CPV-specific serum IgG and virus neutralizing antibody responses. The cellular immune responses analyzed using lymphocyte proliferation test and flow cytometry indicated CPV-specific sensitization of both CD3+CD4+ and CD3+CD8+ lymphocytes. This study demonstrated that the oral CPV DNA vaccine delivered by E. coli can be considered as a promising approach for vaccination of dogs against CPV.
authors.affiliation: Indian Veterinary Research Institute, Izatnagar, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Dahiya
authors.forename: M
authors.initials: M
authors.lastname: Saini
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
mesh_terms: D000284:Administration, Oral; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D004285:Dogs; D004926:Escherichia coli / Q000235:genetics; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation / Q000276:immunology; D010322:Parvoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000276:immunology; D012093:Replicon / Q000235:genetics; D012845:Sindbis Virus / Q000235:genetics*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014765:Viral Vaccines