title: [Relationship of NOR-1 with the regulation of inflammation via liver X receptor alpha in Kupffer cells].
abstract: OBJECTIVE
  To investigate the relationship of NOR-1 with the inhibition of inflammatory reaction in mice Kupffer cells (KCs) induced by lipopolysaccharide (LPS) via liver X receptor alpha (LXR alpha).
  
  
  METHODS
  KCs from male KM mice were isolated by density gradient centrifugation, incubated and then randomly assigned to three groups: control group, LPS treated group and LPS+T0901317 treated group.
  
  
  RESULTS
  The mRNA and protein expressions of LXR alpha and NOR-1 in each group were determined by RT-PCR, immunofluorescent assay and western blot, respectively. The densities of TNF alpha and IL-10 in supernatants were evaluated by enzyme linked immunosorbent assay (ELISA). The mRNA and protein expression levels of LXR alpha in LPS + T0901317 group were the highest as compared to the other two groups (0.748+/-0.072 and 1.217+/-0.133 respectively), The mRNA and protein expression levels of NOR-1 in LPS+ T0901317 group were the highest as compared to the other two groups (2.726+/-0.065 and 0.842+/-0.058 respectively). The densities of supernatant TNF alpha in LPS group and IL-10 in LPS+T0901317 group were the highest [(450.89+/-78.52) ng/L and (537.41+/-36.41) ng/L respectively].
  
  
  CONCLUSIONS
  Promoting the expression of LXR alpha in KCs can elevate the NOR-1 expression and then inhibit inflammatory reaction.
authors.affiliation: Department of Hepatobiliary Surgery, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Zhuo-ya
authors.initials: ZY
authors.lastname: Dai
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Si-dong
authors.initials: SD
authors.lastname: Wei
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D007728:Kupffer Cells / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D057093:Orphan Nuclear Receptors / Q000378:metabolism*; D011987:Receptors, Steroid / Q000378:metabolism*; D011988:Receptors, Thyroid Hormone / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D000071518:Liver X Receptors; D009419:Nerve Tissue Proteins; C000605214:Nr1h3 protein, mouse; C490411:Nr4a3 protein, mouse; D057093:Orphan Nuclear Receptors; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10