title: Complexes of nucleolipid liposomes with single-stranded and double-stranded nucleic acids.
abstract: We report on the association of anionic liposomes from POP-Ade:POPC (1-palmitoyl-2-oleoyl-phosphatidyladenosine and 1-palmitoyl-2-oleoyl-phosphatidylcholine, respectively) with single- and double-strand nucleic acids, mediated by Ca(2+) bridging. The structural and dynamical features of such complexes are compared with those displayed when the nucleolipid is replaced by POPG (1-palmitoyl-2-oleoyl-sn-phosphatidyl-glycerol), characterized by the same apolar skeleton and negative charge as POP-Ade, but lacking the nucleic polar head. For single-stranded nucleic acids, we demonstrate that specific interactions drive the formation of complexes with nucleolipid liposomes, while no association is present for POPG-based samples. For double-stranded nucleic acids, Ca(2+) bridging promotes association with both liposomal formulations, but the corresponding complexes have different structural features, in terms of size, overall charge and internal liquid-crystalline structure.
authors.affiliation: CSGI and University of Florence, Department of Chemistry, Firenze, Italy.
authors.forename: Costanza
authors.initials: C
authors.lastname: Montis
authors.forename: Silvia
authors.initials: S
authors.lastname: Milani
authors.forename: Debora
authors.initials: D
authors.lastname: Berti
authors.forename: Piero
authors.initials: P
authors.lastname: Baglioni
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D020404:Glycerophospholipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*
chemical_list: C509561:1-palmitoyl-2-oleoyl-phosphatidyl-3-adenosine; D020404:Glycerophospholipids; D008081:Liposomes; D010713:Phosphatidylcholines; D004247:DNA; D000241:Adenosine; D002118:Calcium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine