title: Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.
abstract: Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis. Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure.
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-CSIC, Villaviciosa, Asturias, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Ruiz
authors.forename: Aldert
authors.initials: A
authors.lastname: Zomer
authors.forename: Mary
authors.initials: M
authors.lastname: O'Connell-Motherway
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001644:Bifidobacterium / Q000235:genetics* / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D023281:Genomics / Q000379:methods; D013294:Lactococcus lactis / Q000235:genetics; D050296:Microbial Viability / Q000187:drug effects; D059467:Transcriptome
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts