title: An unlikely role for the NAT2 genotypes and haplotypes in the oral cancer of south Indians.
abstract: The arylamine N-acetyltransferase 2 (NAT2) enzyme detoxifies a wide spectrum of naturally occurring xenobiotics including carcinogens and drugs. Acetylation catalysed by the NAT2 is an important process in metabolic activation of arylamines to electrophilic intermediates that initiate carcinogenesis. Polymorphism in N-acetyltransferase 2 gene was reported to be associated with the susceptibility of various cancers.
  
  
  OBJECTIVE
  The aim of our study was to determine whether there is any association between the susceptibility to oral cancer amongst the variations of NAT2 genotypes.
  
  
  METHODS
  This study was carried out in 157 patients with oral cancer. The control group consisted of 132 healthy volunteers. The most common polymorphisms rs1799929, rs1799930 and rs1799931 on the NAT2 gene were screened for the genotypes using TaqMan allelic discrimination.
  
  
  RESULTS
  All the three SNPs were polymorphic with minor allele frequency of 0.339, 0.372 and 0.061 for rs1799929, rs1799930 and rs1799931, respectively. None of the polymorphic site deviated from HWE in controls. There were no significant differences in genotype or allele frequencies of three SNPs between controls and cases with oral cancer. Risk of oral cancer development for the carriers of the individual deduced phenotypes was also not statistically significant. Of the 3 studied polymorphisms, 2 were in strong LD and form one haplotype block. None of the haplotype had shown significant association with the oral cancer.
  
  
  CONCLUSIONS
  Our study concludes that the NAT2 genotypes, phenotypes and haplotypes are not involved in the susceptibility to oral cancer in South Indian subjects.
authors.affiliation: Department of Endodontics, Sri Ramachandra Dental College and Hospital, Sri Ramachandra University, Chennai, India.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Balaji
authors.forename: Balaji Singh
authors.initials: BS
authors.lastname: Krishna
authors.forename: L V K S
authors.initials: LV
authors.lastname: Bhaskar
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007194:India; D016015:Logistic Models; D009062:Mouth Neoplasms / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012306:Risk
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human