title: c-Jun induction is independent of early growth response factor during cerebellar granule neuron apoptosis.
abstract: Early growth response factor 1 (Egr-1) is induced during activity deprivation-dependent apoptosis in cerebellar granule neurons and plays a critical role in neuronal apoptosis. It has been proposed that c-Jun lies downstream of the Egr-dependent proapoptotic signaling cascade in cerebellar granule neurons. However, our data show that neither suppressing Egr activity using a dominant-negative mutant nor specifically silencing Egr-1 using small interfering RNA inhibited the activation and expression of c-Jun after activity deprivation in cerebellar granule neurons. Furthermore, two inhibitors, mithramycin A and chromomycin A3, which repressed transcription by selectively displacing GC-rich DNA-binding transcription factors (including those of the Egr family), did not attenuate the activation and expression of c-Jun during neuronal apoptosis. We conclude that c-Jun induction is independent of Egr during apoptosis induced by activity deprivation in cerebellar granule neurons.
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.forename: Bo
authors.initials: B
authors.lastname: Xie
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Mingtao
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002531:Cerebellum / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D034741:RNA, Small Interfering; D048031:JNK Mitogen-Activated Protein Kinases