title: Molecular characterization of sweet potato leaf curl virus (SPLCV) isolates from Korea: phylogenetic relationship and recombination analysis.
abstract: The complete DNA genome of sweet potato leaf curl virus (SPLCV) from samples obtained from eight regions was amplified by PCR and characterized in this study. The DNA genome of one group (SPLCV Korea group 1) consisted of 2828 nucleotides and that of the second group (SPLCV Korea group 2) consisted of 2829 nucleotides. Sequence comparisons showed that the genome sequences of SPLCV Korea isolates were closely related to those of SPLCV Brazil isolates (FJ969834, FJ969835, and FJ969836), SPLCV Japan isolate (AB433788), and SPLCV USA isolate (AF104036) with nucleotide sequence identity values ranging from 96-98%. Analysis of the phylogenetic relationship of SPLCV Korea isolates with other begomoviruses revealed that the majority of SPLCV Korea isolates were clustered with SPLCV Brazil isolates (FJ969834, FJ969835, and FJ969836). Recombination analysis results revealed three recombinations among SPLCV Korea isolates, SPLCV isolates from Brazil and Japan, and ipomoea yellow vein virus (IYVV) Italy isolate.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: E
authors.initials: E
authors.lastname: Choi
authors.forename: H-R
authors.initials: HR
authors.lastname: Kwak
authors.forename: M-K
authors.initials: MK
authors.lastname: Kim
authors.forename: K-Y
authors.initials: KY
authors.lastname: Lee
authors.forename: H-S
authors.initials: HS
authors.lastname: Choi
authors.forename: S
authors.initials: S
authors.lastname: Lee
mesh_terms: D053839:Begomovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016679:Genome, Viral; D027723:Ipomoea batatas / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral