title: Cordycepin inhibits albumin-induced epithelial-mesenchymal transition of renal tubular epithelial cells by reducing reactive oxygen species production.
abstract: Albumin induced epithelial-mesenchymal transition (EMT) of renal tubular cells through reactive oxygen species (ROS) pathway plays an important role in tubulointerstitial fibrosis. Cordycepin (3 -deoxyadenosine), a potential antioxidant, was demonstrated to have various pharmacological effects and could inhibit EMT of some cells. However, the role of cordycepin on albumin-induced EMT in renal tubular cells (HK2) is unclear. In this study, we investigated the effect of cordycepin on albumin-induced EMT of HK2 cells and its mechanisms. HK-2 cells were exposed to bovine serum albumin with or without pretreatment with cordycepin. Results showed that albumin significantly induced EMT formation of HK-2 which associated with NADPH oxidase activation and intracellular ROS overproduction through increased Rac1 activity and expression of NOX4, p22phox and p47phox, while these effects were abolished in that pretreated with cordycepin. In conclusion, cordycepin could ameliorate albumin-induced EMT of HK2 cells by decreasing NADPH oxidase activity and inhibiting ROS production.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Ming
authors.initials: M
authors.lastname: Zhan
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Shaobin
authors.initials: S
authors.lastname: Duan
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D003839:Deoxyadenosines / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D014158:Transcription, Genetic / Q000187:drug effects; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D003839:Deoxyadenosines; D021122:Protein Subunits; C107953:RAC1 protein, human; D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D019255:NADPH Oxidases; D020830:rac1 GTP-Binding Protein; C058120:cordycepin