title: Enhancement of vindoline production in suspension culture of the Catharanthus roseus cell line C20hi by light and methyl jasmonate elicitation.
abstract: The effects of light and methyl jasmonate (MJ) on the transcription of biosynthetic genes as well as the accumulation of vindoline and catharanthine in Catharanthus roseus C20hi cell suspensions were studied. t16h (the gene encoding tabersonine 16-hydroxylase) could be induced by light and MJ, whereas d4h (the gene encoding deacetoxyvindoline 4-hydroxylase) could only be induced by light. Quantification by UPLC-MS showed that light significantly increased vindoline production in C20hi cells by about 0.49 - 5.51-fold more than that in controls, with the highest yield being 75.3 ng/g of dry weight. The biosynthesis of vindoline was further enhanced by combining MJ with light. The accumulation of catharanthine was not improved by either light or MJ elicitation. These results suggested that light and MJ could promote vindoline, but not catharanthine accumulation in C20hi cells.
authors.affiliation: The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Ronghui
authors.initials: R
authors.lastname: Tan
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhao
authors.forename: Zhibi
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001483:Base Sequence; D030008:Catharanthus / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000378:metabolism*; D017931:DNA Primers; D008027:Light; D054883:Oxylipins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D017931:DNA Primers; D054883:Oxylipins; C009667:vindoline; D014747:Vinblastine; C072239:methyl jasmonate