title: Isotopically modified nanoparticles for enhanced detection in bioaccumulation studies.
abstract: This work presents results on synthesis of isotopically enriched (99% (65)Cu) copper oxide nanoparticles and its application in ecotoxicological studies. (65)CuO nanoparticles were synthesized as spheres (7 nm) and rods (7 × 40 nm). Significant differences were observed between the reactivity and dissolution of spherical and rod shaped nanoparticles. The extreme sensitivity of the stable isotope tracing technique developed in this study allowed determining Cu uptake at exposure concentrations equivalent to background Cu concentrations in freshwater systems (0.2-30 μg/L). Without a tracer, detection of newly accumulated Cu was impossible, even at exposure concentrations surpassing some of the most contaminated water systems (>1 mg/L).
authors.affiliation: Natural History Museum, Dept. of Mineralogy, Cromwell Road, London SW7 5BD, United Kingdom. s.misra@nhm.ac.uk
authors.forename: Superb K
authors.initials: SK
authors.lastname: Misra
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Dybowska
authors.forename: Deborah
authors.initials: D
authors.lastname: Berhanu
authors.forename: Marie Noële
authors.initials: MN
authors.lastname: Croteau
authors.forename: Samuel N
authors.initials: SN
authors.lastname: Luoma
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Eugenia
authors.initials: E
authors.lastname: Valsami-Jones
mesh_terms: D000818:Animals; D001692:Biological Transport; D003300:Copper / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007554:Isotopes; D008195:Lymnaea / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D007554:Isotopes; D003300:Copper