title: ABPP methodology: introduction and overview.
abstract: Activity-based protein profiling (ABPP) is emerging as a mature method for chemically interrogating the proteome of a cell. This chapter serves to introduce the reader to ABPP by providing overviews of the general principles of the technique, analytical methods used in ABPP, the classes of enzymes that can be specifically addressed by ABPP probes, and biological applications of ABPP.
authors.affiliation: Department Chemie, Centre for Integrated Protein Science CIPSM, Institute of Advanced Studies, Technische Universität München, Lichtenbergstrasse 4, 85747, Garching, Germany.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Nodwell
authors.forename: Stephan A
authors.initials: SA
authors.lastname: Sieber
mesh_terms: D006801:Humans; D015335:Molecular Probes; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D015335:Molecular Probes; D011506:Proteins; D020543:Proteome