title: An antifungal protein from the culinary-medicinal beech mushroom, Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae).
abstract: An antifungal protein (HM-af) was purified from the culinary-medicinal mushroom Hypsizygus marmoreus. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of HM-af indicated that its molecular mass was 9.5 kDa. The N-terminal amino acid sequence of HM-af showed homology to ribonuclease H from Clostridium thermocellum. HM-af showed the antifungal activity against Flammulina velutipes.
authors.affiliation: Innovation and Joint Research Center, Shizuoka University, Suruga-ku, Shizuoka, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kanako
authors.initials: K
authors.lastname: Umehara
authors.forename: Ai
authors.initials: A
authors.lastname: Tashiro
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Hideo
authors.initials: H
authors.lastname: Dohra
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001681:Biological Assay; D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D055393:Flammulina / Q000187:drug effects* / Q000254:growth & development; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008970:Molecular Weight; D010449:Peptide Mapping; D016914:Ribonuclease H / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000935:Antifungal Agents; D045424:Complex Mixtures; D005656:Fungal Proteins; C569052:HM-af protein, Hypsizygus marmoreus; D016914:Ribonuclease H