title: [Optimized procedure for fluorescence in situ hybridization in rapid prenatal diagnosis of common aneuploidy].
abstract: OBJECTIVE
  To optimize the procedure of fluorescence in situ hybridization (FISH), and evaluate it in rapid prenatal diagnosis of common aneuploidy.
  
  
  METHODS
  Amniotic fluid samples from 300 pregnant women were tested by both interphase FISH and conventional cell culture for karyotyping from September 2009 and September 2010.
  
  
  RESULTS
  Seven cases of trisomy 21, 4 of trisomy 18, 2 of monosomy X, 1 of XXY, 1 of XXX, and 1 of triploidy were detected by FISH in the 300 amniotic fluid samples. It was concordant with the results from conventional karyotype analysis. The concordance rate was 100%.
  
  
  CONCLUSIONS
  Through a technical modification of FISH procedure, the detection accuracy and specificity was not affected but testing cost reduced greatly. It can be used in rapid prenatal diagnosis of common aneuploidy.
authors.affiliation: Department of Obstetrics and Gynecology, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology; D000782:Aneuploidy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D055815:Young Adult