title: Defining function of lipopolysaccharide O-antigen ligase WaaL using chemoenzymatically synthesized substrates.
abstract: The WaaL-mediated ligation of O-antigen onto the core region of the lipid A-core block is an important step in the lipopolysaccharide (LPS) biosynthetic pathway. Although the LPS biosynthesis has been largely characterized, only a limited amount of in vitro biochemical evidence has been established for the ligation reaction. Such limitations have primarily resulted from the barriers in purifying WaaL homologues and obtaining chemically defined substrates. Accordingly, we describe herein a chemical biology approach that enabled the reconstitution of this ligation reaction. The O-antigen repeating unit (O-unit) of Escherichia coli O86 was first enzymatically assembled via sequential enzymatic glycosylation of a chemically synthesized GalNAc-pyrophosphate-undecaprenyl precursor. Subsequent expression of WaaL through use of a chaperone co-expression system then enabled the demonstration of the in vitro ligation between the synthesized donor (O-unit-pyrophosphate-undecaprenyl) and the isolated lipid A-core acceptor. The previously reported ATP and divalent metal cation dependence were not observed using this system. Further analyses of other donor substrates revealed that WaaL possesses a highly relaxed specificity toward both the lipid moiety and the glycan moiety of the donor. Lastly, three conserved amino acid residues identified by sequence alignment were found essential for the WaaL activity. Taken together, the present work represents an in vitro systematic investigation of the WaaL function using a chemical biology approach, providing a system that could facilitate the elucidation of the mechanism of WaaL-catalyzed ligation reaction.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia 30302, USA.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Han
authors.forename: Baolin
authors.initials: B
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhao
authors.forename: Robert
authors.initials: R
authors.lastname: Woodward
authors.forename: Nicholas
authors.initials: N
authors.lastname: Pettit
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Peng G
authors.initials: PG
authors.lastname: Wang
mesh_terms: D019729:Carbon-Oxygen Ligases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D011756:Diphosphates / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation; D019081:O Antigens / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011756:Diphosphates; D029968:Escherichia coli Proteins; D019081:O Antigens; C107241:diphosphoric acid; D019729:Carbon-Oxygen Ligases; C571195:WaaL protein, E coli