title: Enzymatic synthesis of 4-pentulosonate (4-keto-D-pentonate) from D-aldopentose and D-pentonate by two different pathways using membrane enzymes of acetic acid bacteria.
abstract: 4-Keto-D-arabonate (D-threo-pent-4-ulosonate) and 4-keto-D-ribonate (D-erythro-pent-4-ulosonate) were prepared from D-arabinose and D-ribose by two successive reactions of membrane-bound enzymes, D-aldopentose 4-dehydrogenase and 4-keto-D-aldopentose 1-dehydrogenase of Gluconobacter suboxydans IFO 12528. Alternatively, they were prepared from D-arabonate and D-ribonate with another membrane-bound enzyme, D-pentonate 4-dehydrogenase. Analytical data confirmed the chemical structures of the 4-pentulosonates prepared. This is the first report of successful enzymatic synthesis of 4-pentulosonates.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. osao@yamaguchi-u.ac.jp
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Roque A
authors.initials: RA
authors.lastname: Hours
authors.forename: Emiko
authors.initials: E
authors.lastname: Shinagawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Akakabe
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D020576:Gluconobacter / Q000166:cytology* / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D010429:Pentoses / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*
chemical_list: D010429:Pentoses; D013400:Sugar Acids; D010088:Oxidoreductases; D019342:Acetic Acid