title: Applications of differential scanning calorimetry for thermal stability analysis of proteins: qualification of DSC.
abstract: Differential scanning calorimetry (DSC) has been used to characterize protein thermal stability, overall conformation, and domain folding integrity by the biopharmaceutical industry. Recently, there have been increased requests from regulatory agencies for the qualification of characterization methods including DSC. Understanding the method precision can help determine what differences between samples are significant and also establish the acceptance criteria for comparability and other characterization studies. In this study, we identify the parameters for the qualification of DSC for thermal stability analysis of proteins. We use these parameters to assess the precision and sensitivity of DSC and demonstrate that DSC is suitable for protein thermal stability analysis for these purposes. Several molecules from different structural families were studied. The experiments and data analyses were performed by different analysts using different instruments at different sites. The results show that the (apparent) thermal transition midpoint (T(m)) values obtained for the same protein by same and different instruments and/or analysts are quite reproducible, and the profile similarity values obtained for the same protein from the same instrument are also high. DSC is an appropriate method for assessing protein thermal stability and conformational changes.
authors.affiliation: Product Attribute Sciences, Amgen Inc., Thousand Oaks, California 91320, USA. jwen@amgen.com
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.forename: Kelly
authors.initials: K
authors.lastname: Arthur
authors.forename: Letha
authors.initials: L
authors.lastname: Chemmalil
authors.forename: Salman
authors.initials: S
authors.lastname: Muzammil
authors.forename: John
authors.initials: J
authors.lastname: Gabrielson
authors.forename: Yijia
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods*; D011487:Protein Conformation; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D011506:Proteins