title: Development and validation of a reversed-phase column liquid chromatographic method for the determination of five cephalosporins in pharmaceutical preparations.
abstract: A new, simple, rapid, and precise RP-HPLC method has been developed and validated for the determination of five cephalosporins, namely, cefalexin, cefoperazone, ceftriaxone, ceftazidime, and cefepime. The method has been applied successfully for simultaneous determination of cefalexin in a binary mixture with sodium benzoate in a suspension, and cefoperazone in a binary mixture with sulbactam in vials. Chromatographic separation was achieved on a Waters microBondapak C18 column (250 x 4.6 mm id, 10 pm particle size) using the mobile phase monobasic potassium phosphate (50 mM, pH 4.6)-acetonitrile (80 + 20, v/v) with UV detection. A flow rate of 1 mL/min was applied. Linearity, accuracy, and precision were found to be acceptable over the concentration range of 30-300, 3-30, and 15-120 microg/mL for the studied cephalosporins, sodium benzoate, and sulbactam, respectively. The optimized method proved to be specific, robust, and accurate for QC of the cited drugs in their pharmaceutical preparations.
authors.affiliation: Cairo University, Faculty of Pharmacy, Pharmaceutical Chemistry Department, Kasr El-Aini St, Cairo 11562, Egypt. ehabelkady75@yahoo.com
authors.forename: Ehab F
authors.initials: EF
authors.lastname: Elkady
authors.forename: Samah S
authors.initials: SS
authors.lastname: Abbas
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002214:Capsules / Q000032:analysis; D002511:Cephalosporins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D007202:Indicators and Reagents; D057230:Limit of Detection; D019999:Pharmaceutical Solutions / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013407:Sulbactam / Q000032:analysis; D013535:Suspensions / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D002214:Capsules; D002511:Cephalosporins; D004338:Drug Combinations; D007202:Indicators and Reagents; D019999:Pharmaceutical Solutions; D013535:Suspensions; D013407:Sulbactam