title: Alterations in promoter methylation status of tumor suppressor HIC1, SFRP2, and DAPK1 genes in prostate carcinomas.
abstract: Hypermethylated genomic DNA is a common feature in tumoral tissues, although the prevalence of this modification remains poorly understood. We aimed to determine the frequency of five tumor suppressor (TS) genes in prostate cancer and the correlation between promoter hypermethylation of these genes and low and high grade of prostate carcinomas. A total of 30 prostate tumor specimens were investigated for promoter methylation status of TS hypermethylated in cancer 1 (HIC1), death-associated protein kinase 1 (DAPK1), secreted frizzled-related protein 2 (SFRP2), cyclin-dependent kinase inhibitor 2A (p16), and O-6-methylguanine-DNA methyltransferase (MGMT) genes by using bisulfite modifying method. A high frequency of promoter hypermethylation was found in HIC1 (70.9%), SFRP2 (58.3%), and DAPK1 (33.3%) genes in tumor samples that were examined. The current data show high frequency of hypermethylation changes in HIC1, SFRP2, and DAPK1 genes in prostate carcinomas of high Gleason Score (GS).
authors.affiliation: Department of Urology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
authors.forename: Devran
authors.initials: D
authors.lastname: Kilinc
authors.forename: Oztürk
authors.initials: O
authors.lastname: Ozdemir
authors.forename: Semra
authors.initials: S
authors.lastname: Ozdemir
authors.forename: Esat
authors.initials: E
authors.lastname: Korgali
authors.forename: Binnur
authors.initials: B
authors.lastname: Koksal
authors.forename: Atilla
authors.initials: A
authors.lastname: Uslu
authors.forename: Yener E
authors.initials: YE
authors.lastname: Gultekin
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D019175:DNA Methylation; D064550:Death-Associated Protein Kinases; D005838:Genotype; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012307:Risk Factors; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D051017:Apoptosis Regulatory Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004247:DNA; D064550:Death-Associated Protein Kinases; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins; C499808:HIC1 protein, human; C109618:SFRP2 protein, human; D000094742:Secreted Frizzled-Related Proteins; C579096:DAPK1 protein, human