title: Microwave-assisted purge-and-trap extraction device coupled with gas chromatography and mass spectrometry for the determination of five predominant odors in sediment, fish tissues, and algal cells.
abstract: Off-flavors are among the most troublesome compounds in the environment worldwide. The lack of a viable theory for studying the sources, distribution, and effect of odors has necessitated the accurate measurement of odors from environmental compartments. A rapid and flexible microwave-assisted purge-and-trap extraction device for simultaneously determining five predominant odors, namely, dimethyltrisulfide, 2-methylisoborneol, geosmin, β-cyclocitral and β-ionone, from the primary sources and sinks is demonstrated. This instrument facilitates the extraction and concentration of odors from quite different matrices simultaneously. This device is a solvent-free automated system that does not require cleaning and is timesaving. The calibration curves of the five odor compounds showed good linearity in the range of 1-500 ng/L, with correlation coefficients above 0.999 (levels=7) and with residuals ranging from approximately 77% to 104%. The limits of detection (S/N=3) were below 0.15 ng/L in algae sample and 0.07 ng/g in sediment and fish tissue samples. The relative standard deviations were between 2.65% and 7.29% (n=6). Thus the proposed design is ready for rapid translation into a standard analytical tool and is useful for multiple applications in the analysis of off-flavors.
authors.affiliation: Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Chinese Academy of Sciences, Wuhan, Hubei, China.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Deng
authors.forename: Ping
authors.initials: P
authors.lastname: Xie
authors.forename: Min
authors.initials: M
authors.lastname: Qi
authors.forename: Gaodao
authors.initials: G
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000818:Animals; D001892:Camphanes / Q000032:analysis; D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods*; D000460:Chlorophyta / Q000737:chemistry; D004224:Diterpenes / Q000032:analysis; D004867:Equipment Design; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008872:Microwaves; D009284:Naphthols / Q000032:analysis; D045792:Norisoprenoids / Q000032:analysis; D009812:Odorants / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D000447:Aldehydes; D001892:Camphanes; D004224:Diterpenes; D009284:Naphthols; D045792:Norisoprenoids; C005536:2-methylisoborneol; C516118:beta-cyclocitral; C008157:beta-ionone; C001278:geosmin