title: Preparation and investigation of Ulex europaeus agglutinin I-conjugated liposomes as potential oral vaccine carriers.
abstract: We prepared and optimized Ulex europaeus agglutinin I (UEAI)-modified Bovine serum albumin (BSA)-encapsulating liposomes (UEAI-LIP) as oral vaccine carriers and examined the feasibility of inducing systemic and mucosal immune responses by oral administration of UEAILIP. The prepared systems were characterized in vitro for their average size, zeta potential, encapsulation efficiency (EE%) and conjugation efficiency (CE%). In vitro release studies indicated that the presence of UEAI around the optimized liposomes was able to prevent a burst release of loaded BSA and provide sustained release of the encapsulated protein. In vivo immune-stimulating results in KM mice showed that BSA given intramuscularly generated systemic response only but both systemic and mucosal immune responses could be induced simultaneously in the groups in which BSA-loaded liposomes (LIP) and UEAI-LIP were administered intragastrically. Furthermore, the modification of UEAI on the surface of liposomes could further enhance the IgA and IgG levels obviously. In conclusion, this study demonstrated the high potential of lectin-modified liposomes containing the antigen as carriers for oral vaccine.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.
authors.forename: KeXin
authors.initials: K
authors.lastname: Li
authors.forename: DaWei
authors.initials: D
authors.lastname: Chen
authors.forename: XiuLi
authors.initials: X
authors.lastname: Zhao
authors.forename: HaiYang
authors.initials: H
authors.lastname: Hu
authors.forename: ChunRong
authors.initials: C
authors.lastname: Yang
authors.forename: DaHai
authors.initials: D
authors.lastname: Pang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D055598:Chemical Phenomena; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D004339:Drug Compounding; D005240:Feasibility Studies; D005260:Female; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D037121:Plant Lectins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013047:Specific Pathogen-Free Organisms; D013535:Suspensions; D039762:Ulex / Q000378:metabolism; D053835:Unilamellar Liposomes; D014612:Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D037121:Plant Lectins; D013535:Suspensions; C036850:Ulex europaeus lectins; D053835:Unilamellar Liposomes; D014612:Vaccines; D012710:Serum Albumin, Bovine