title: Antioxidant, anti-glycation and anti-inflammatory activities of phenolic constituents from Cordia sinensis.
abstract: Nine compounds have been isolated from the ethyl acetate soluble fraction of C. sinensis, namely protocatechuic acid (1), trans-caffeic acid (2), methyl rosmarinate (3), rosmarinic acid (4), kaempferide-3-O-β-D-glucopyranoside (5), kaempferol-3-O-β-D-glucopyranoside (6), quercetin-3-O-β-D-glucopyranoside (7), kaempferide-3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (8) and kaempferol-3-O-α-L-rhamno-pyranosyl (1→6)-β-D-glucopyranoside (9), all reported for the first time from this species. The structures of these compounds were deduced on the basis of spectroscopic studies, including 1D and 2D NMR techniques. Compounds 1-9 were investigated for biological activity and showed significant anti-inflammatory activity in the carrageen induced rat paw edema test. The antioxidant activities of isolated compounds 1-9 were evaluated by the DPPH radical scavenging test, and compounds 1, 2, 4 and 7-9 exhibited marked scavenging activity compared to the standard BHA. These compounds were further studied for their anti-glycation properties and some compounds showed significant anti-glycation inhibitory activity. The purity of compounds 2-5, 8 and 9 was confirmed by HPLC. The implications of these results for the chemotaxonomic studies of the genus Cordia have also been discussed.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
authors.forename: Nawal
authors.initials: N
authors.lastname: Al-Musayeib
authors.forename: Shagufta
authors.initials: S
authors.lastname: Perveen
authors.forename: Itrat
authors.initials: I
authors.lastname: Fatima
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nasir
authors.forename: Ajaz
authors.initials: A
authors.lastname: Hussain
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D031213:Cordia / Q000737:chemistry* / Q000145:classification; D004487:Edema / Q000188:drug therapy; D006031:Glycosylation / Q000187:drug effects; D020128:Inhibitory Concentration 50; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl