title: Molecular and immunological characterization of Mus a 5 allergen from banana fruit.
abstract: METHODS
  Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed.
  
  
  RESULTS
  The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner.
  
  
  CONCLUSIONS
  Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Aleksic
authors.forename: Milica
authors.initials: M
authors.lastname: Popovic
authors.forename: Rajna
authors.initials: R
authors.lastname: Dimitrijevic
authors.forename: Uros
authors.initials: U
authors.lastname: Andjelkovic
authors.forename: Emilia
authors.initials: E
authors.lastname: Vassilopoulou
authors.forename: Athanassios
authors.initials: A
authors.lastname: Sinaniotis
authors.forename: Marina
authors.initials: M
authors.lastname: Atanaskovic-Markovic
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Arnd
authors.initials: A
authors.lastname: Petersen
authors.forename: Nikolaos G
authors.initials: NG
authors.lastname: Papadopoulos
authors.forename: Marija
authors.initials: M
authors.lastname: Gavrovic-Jankulovic
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D003001:Cloning, Molecular; D044644:Endo-1,3(4)-beta-Glucanase / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D028521:Musa / Q000737:chemistry*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D020033:Protein Isoforms / Q000737:chemistry; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism; D012882:Skin Tests / Q000379:methods; D060149:Tetraspanin 30 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C554586:CD63 protein, human; C427944:ENPP3 protein, human; D010940:Plant Proteins; D020033:Protein Isoforms; D060149:Tetraspanin 30; D007073:Immunoglobulin E; D010727:Phosphoric Diester Hydrolases; D044644:Endo-1,3(4)-beta-Glucanase; D011755:Pyrophosphatases