title: Suppression of nasopharyngeal carcinoma cell by targeting β-catenin signaling pathway.
abstract: OBJECTIVE
  To investigate the role of β-catenin in pathogenesis of nasopharyngeal carcinoma (NPC).
  
  
  METHODS
  Cellular proliferation, apoptosis, matrix penetration assay, and western blotting were employed to determine cell biological changes in NPC cell lines transfected with β-catenin siRNA. Immunohistochemistry staining was used to detect β-catenin and Ki-67 expression in NPC tissue.
  
  
  RESULTS
  β-Catenin was upregulated in NPC cell lines and tissues compared with chronic nasopharyngitis tissue. β-Catenin knockdown dramatically inhibited cellular growth, migration and invasion, but induced apoptosis of NPC cells. Further study showed that downstream genes of β-catenin signaling pathway including cyclin D1, c-Myc, MMP2 and MMP9 expression were suppressed in NPC cell lines transfected with β-catenin siRNA.
  
  
  CONCLUSIONS
  Targeting β-catenin signaling pathway may be a noval strategy for NPC therapy.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Song
authors.forename: Qing Xu
authors.initials: QX
authors.lastname: Yang
authors.forename: Fenfen
authors.initials: F
authors.lastname: Zhang
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Meng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.forename: Anjia
authors.initials: A
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002277:Carcinoma; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D034741:RNA, Small Interfering; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D015854:Up-Regulation; D055815:Young Adult; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D034741:RNA, Small Interfering; D051176:beta Catenin