title: Physiological and biochemical metabolism of germinating broccoli seeds and sprouts.
abstract: Changes in physiological and biochemical metabolism as well as glucoraphanin and sulforaphane contents of germinating broccoli seeds and sprouts were investigated in this study. Sprout length, root length, and fresh weight increased with germination time. Dry weight varied from 2.5 to 3.0 mg per sprout. A rapid increase in respiratory rate of sprouts occurred between 24 and 36 h of germination and then stayed at a high level. HPLC analysis found that glucoraphanin content increased at the early stage (0-12 h) of germination, decreased to a low value of 3.02 mg/g at 48 h, and then reached the highest value of 6.30 mg/g at 72 h of germination. Sulforaphane content decreased dramatically during the first day of germination, then increased slowly, and reached a high value of 3.38 mg/g at 48 h before declining again.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Gu
authors.forename: Qianghui
authors.initials: Q
authors.lastname: Guo
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Chen
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Han
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Gu
mesh_terms: D001937:Brassica / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D018525:Germination; D005961:Glucosinolates / Q000032:analysis / Q000378:metabolism; D007096:Imidoesters / Q000032:analysis / Q000378:metabolism; D017879:Isothiocyanates; D010091:Oximes; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D013454:Sulfoxides; D013861:Thiocyanates / Q000032:analysis / Q000378:metabolism
chemical_list: D005961:Glucosinolates; D007096:Imidoesters; D017879:Isothiocyanates; D010091:Oximes; D010936:Plant Extracts; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane; C119494:glucoraphanin