title: Characterization of a loss-of-function mutant of gibberellin biosynthetic gene LsGA3ox1 in lettuce.
abstract: A previous study generated lettuce (Lactuca sativa) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3ox1 in Tnt6a mutants. Expression analysis suggests that LsGA3ox1 is nearly knocked out in the Tnt6a mutants.
authors.affiliation: Department of Bioresource Engineering, Yamagata University, Tsuruoka, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Umetsu
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sawada
authors.forename: Wataru
authors.initials: W
authors.lastname: Mitsuhashi
authors.forename: Marianne
authors.initials: M
authors.lastname: Mazier
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Toyomasu
mesh_terms: D001483:Base Sequence; D017343:Genes, Plant / Q000235:genetics*; D005875:Gibberellins / Q000096:biosynthesis*; D007438:Introns / Q000235:genetics; D018545:Lactuca / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D018626:Retroelements / Q000235:genetics
chemical_list: D005875:Gibberellins; D018626:Retroelements