title: Magnetic silica nanoparticle cellular uptake and cytotoxicity regulated by electrostatic polyelectrolytes-DNA loading at their surface.
abstract: Magnetic silica nanoparticles show great promise for drug delivery. The major advantages correspond to their magnetic nature and ease of biofunctionalization, which favors their ability to interact with cells and tissues. We have prepared magnetic silica nanoparticles with DNA fragments attached on their previously polyelectrolyte-primed surface. The remarkable feature of these materials is the compromise between the positive charges of the polyelectrolytes and the negative charges of the DNA. This dual-agent formulation dramatically changes the overall cytotoxicity and chemical degradation of the nanoparticles, revealing the key role that surface functionalization plays in regulating the mechanisms involved.
authors.affiliation: Departamento de Física Aplicada, Universidade de Vigo, 36310, Vigo, Spain.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Davila-Ibanez
authors.forename: Veronica
authors.initials: V
authors.lastname: Salgueirino
authors.forename: Vicenta
authors.initials: V
authors.lastname: Martinez-Zorzano
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mariño-Fernández
authors.forename: Andres
authors.initials: A
authors.lastname: García-Lorenzo
authors.forename: Melodie
authors.initials: M
authors.lastname: Maceira-Campos
authors.forename: Monica
authors.initials: M
authors.lastname: Muñoz-Ubeda
authors.forename: Elena
authors.initials: E
authors.lastname: Junquera
authors.forename: Emilio
authors.initials: E
authors.lastname: Aicart
authors.forename: Jose
authors.initials: J
authors.lastname: Rivas
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Rodriguez-Berrocal
authors.forename: Jose L
authors.initials: JL
authors.lastname: Legido
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects*; D004247:DNA / Q000737:chemistry* / Q000494:pharmacology*; D004058:Diffusion; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D004247:DNA