title: Cloning and heterologous expression of the vibrioferrin biosynthetic gene cluster from a marine metagenomic library.
abstract: A biosynthetic gene cluster of siderophore consisting of five open reading frames (ORFs) was cloned by functional screening of a metagenomic library constructed from tidal-flat sediment. Expression of the cloned biosynthetic genes in Escherichia coli led to the production of vibrioferrin, a siderophore originally reported for the marine bacterium Vibrio parahaemolyticus. To the best of our knowledge, this is the first example of heterologous production of a siderophore by biosynthetic genes cloned from a metagenomic library. The cloned cluster was one of the largest of the clusters obtained by functional screening. In this study, we demonstrated and extended the possibility of function-based metagenomic research.
authors.affiliation: Priority Organization for Innovation and Excellence, Kumamoto University, Japan. fujitam@kumamoto-u.ac.jp
authors.forename: Masaki J
authors.initials: MJ
authors.lastname: Fujita
authors.forename: Nobutada
authors.initials: N
authors.lastname: Kimura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sakai
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hanyu
authors.forename: Masami
authors.initials: M
authors.lastname: Otsuka
mesh_terms: D059001:Aquatic Organisms / Q000235:genetics*; D002951:Citrates / Q000096:biosynthesis*; D003001:Cloning, Molecular; D015870:Gene Expression; D015698:Genomic Library; D056186:Metagenomics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011760:Pyrrolidinones; D017262:Siderophores / Q000096:biosynthesis*; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000378:metabolism
chemical_list: D002951:Citrates; D011760:Pyrrolidinones; D017262:Siderophores; C090126:vibrioferrin