title: Generation of mice with conditional null allele for GdX/Ubl4A.
abstract: GdX (also named Ubl4A) is a house-keeping gene located on the X chromosome and encodes a protein harboring an ubiquitin-like domain in human and mouse. Although identified in 1988, the function of GdX remains unknown. To elucidate the role of GdX in vivo, we generated a conditional GdX knockout mouse in which Exon 2 was flanked by two loxP sites. We obtained viable and fertile mice with homozygous GdX(flox/flox) or GdX(flox/Y) allele. Germ-line transmission was confirmed by crossing the mouse bearing conditionally targeted allele with an EIIα-Cre transgenic mouse. GdX was successfully depleted in tissues of EIIα-Cre-GdX-null mice. GdX(-/-) and GdX(-/Y) mice are viable and exhibit normal development compared with wild-type littermates within 6 months during our observation. We also observed that GdX knockout male mice were functionally normal in the reproductive system where Ubl4B was specifically expressed. GdX(flox/flox) and GdX(flox/Y) conditional mice provide a tool for further tissue-specific function analysis of the GdX protein under different conditions.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yangmeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Dianjun
authors.initials: D
authors.lastname: Wang
authors.forename: Fangli
authors.initials: F
authors.lastname: Ren
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fuyu
authors.initials: F
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Hou
authors.forename: Xuan
authors.initials: X
authors.lastname: Cheng
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wang
authors.forename: Baoqing
authors.initials: B
authors.lastname: Jia
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Chang
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D003433:Crosses, Genetic; D005091:Exons; D005260:Female; D018703:Founder Effect; D020043:Genes, Essential; D050172:Genes, X-Linked; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000172:deficiency / Q000235:genetics; D012098:Reproduction; D012730:Sex Chromosomes; D014452:Ubiquitins / Q000172:deficiency / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D014452:Ubiquitins