title: Delineating diseases by IMS-MS profiling of serum N-linked glycans.
abstract: Altered branching and aberrant expression of N-linked glycans is known to be associated with disease states such as cancer. However, the complexity of determining such variations hinders the development of specific glycomic approaches for assessing disease states. Here, we examine a combination of ion mobility spectrometry (IMS) and mass spectrometry (MS) measurements, with principal component analysis (PCA) for characterizing serum N-linked glycans from 81 individuals: 28 with cirrhosis of the liver, 25 with liver cancer, and 28 apparently healthy. Supervised PCA of combined ion-mobility profiles for several, to as many as 10 different mass-to-charge ratios for glycan ions, improves the delineation of diseased states. This extends an earlier study [J. Proteome Res.2008, 7, 1109-1117] of isomers associated with a single glycan (S(1)H(5)N(4)) in which PCA analysis of the IMS profiles appeared to differentiate the liver cancer group from the other samples. Although performed on a limited number of test subjects, the combination of IMS-MS for different combinations of ions and multivariate PCA analysis shows promise for characterizing disease states.
authors.affiliation: Department of Chemistry, Indiana University , Bloomington, Indiana 47405, United States.
authors.forename: Dragan
authors.initials: D
authors.lastname: Isailovic
authors.forename: Manolo D
authors.initials: MD
authors.lastname: Plasencia
authors.forename: Maissa M
authors.initials: MM
authors.lastname: Gaye
authors.forename: Sarah T
authors.initials: ST
authors.lastname: Stokes
authors.forename: Ruwan T
authors.initials: RT
authors.lastname: Kurulugama
authors.forename: Vitara
authors.initials: V
authors.lastname: Pungpapong
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kyselova
authors.forename: Radoslav
authors.initials: R
authors.lastname: Goldman
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: David E
authors.initials: DE
authors.lastname: Clemmer
mesh_terms: D000293:Adolescent; D000328:Adult; D019295:Computational Biology / Q000379:methods; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*; D008113:Liver Neoplasms / Q000097:blood*; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry / Q000145:classification; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides