title: Protection by [6]-shogaol against lipopolysaccharide-induced toxicity in murine astrocytes is related to production of brain-derived neurotrophic factor.
abstract: [6]-Shogaol has beneficial effects in spinal neuronal regeneration, but associated molecules and mechanisms are not identified. Neurotrophic factors, including brain-derived neurotrophic factor (BDNF), are associated with proliferation and differentiation of neuronal cells and exert a neuroprotective effect in neurodegenerative models. We investigated whether treatment with [6]-shogaol increases BDNF expression in lipopolysaccharide (LPS)-treated astrocytes, and examined the effect of [6]-shogaol on neuronal protection. [6]-Shogaol significantly attenuated the cell death induced by LPS. Western blotting showed that [6]-shogaol treatment reduced Bax expression and increased B-cell lymphoma (Bcl)-2 and BclxL expression in LPS-treated cells, consistent with the effects of BDNF treatment. Furthermore, K252a, a blocker of neurotrophic factors, attenuated the cellular protective effects of [6]-shogaol and BDNF. This study provides the first evidence that [6]-shogaol increases the expression of BDNF in LPS-treated astrocytes. Furthermore, these experimental results indicate that production of BDNF in astrocytes might be related to altered cell viability following [6]-shogaol treatment. Thus, the neuroprotective effects of [6]-shogaol is mediated by up-regulation of BDNF.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Sehwan
authors.initials: S
authors.lastname: Shim
authors.forename: Sokho
authors.initials: S
authors.lastname: Kim
authors.forename: Young-Bae
authors.initials: YB
authors.lastname: Kwon
authors.forename: Jungkee
authors.initials: J
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology*; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002396:Catechols; D008070:Lipopolysaccharides; C040115:shogaol