title: [Hepatic lineage differentiation of hepatic progenitor cells by bone morphogenetic protein or leukemia inhibitory factor].
abstract: OBJECTIVE
  To search for the optimal approach for hepatocyte-directed differentiation of hepatic progenitor cells and investigate the molecular mechanism of the hepatic differentiation.
  
  
  METHODS
  Hepatic progenitor cells were infected with recombinant adenovirus which containing human LIF, BMP2 or BMP9 gene. The maturation and differentiation of progenitor cells were examined by PAS staining and ICG uptake methods at 4, 7 and 10 days post infection. The production of Albumin (Alb) was measured by luciferase activity at day 4, 7, 10 and 14.
  
  
  RESULTS
  PAS staining assay revealed that BMP2 and BMP9 enhanced glycogen storage in hepatic progenitor cells most obviously at day 7. The percentages of positive cells were 30% and 45% respectively at 7 days post-infection. Meanwhile, 40% and 30% cells were positive by ICG uptake assay after BMP2 and BMP9 induction. Luciferase activity indicated that BMP9 induced ALB-Luc activity most significantly at day 7. However, less inductive activity was found in LIF-treated group.
  
  
  CONCLUSIONS
  These results indicated tuat hepatic progenitor cells were differentiated into hepatocyte-like cells by BMPs and LIF induction.
authors.affiliation: The Second Affiliated Hospital and the Key Laboratory of Molecular Biology of Infectious Diseases Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Gao
authors.forename: Xue-fei
authors.initials: XF
authors.lastname: Cai
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Shan
authors.forename: Qing-mei
authors.initials: QM
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.forename: Ni
authors.initials: N
authors.lastname: Tang
mesh_terms: D000256:Adenoviridae; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000821:virology
chemical_list: D019485:Bone Morphogenetic Proteins; D053605:Leukemia Inhibitory Factor