title: High concentration of sodium butyrate suppresses osteoblastic differentiation and mineralized nodule formation in ROS17/2.8 cells.
abstract: Periodontitis is a destructive disease that is likely the result of the activities of different microbial complexes, including anaerobic Gram-negative periodontopathic bacteria. Butyric acid (sodium butyrate; BA) is a major metabolic by-product of anaerobic Gram-negative periodontopathic bacteria present in subgingival plaque. This study was undertaken to examine the effect of BA on the expression of osteogenesis-related transcription factors and mineralized nodule formation in osteoblastic ROS17/2.8 cells. The cells were cultured with 0 (control), 10(-5), 10(-4), or 10(-3) M BA for up to 7 days. The gene and protein expression levels of transcription factors such as Runx2, Osterix, Dlx5, Msx2, and AJ18, as well as extracellular matrix proteins such as bone sialoprotein (BSP) and osteocalcin, were examined using real-time PCR and Western blotting, respectively. Mineralized nodule formation was detected by alizarin red staining. The expression of Runx2, Osterix, Dlx5, and Msx2 decreased significantly in the presence of 10(-3 )M BA compared to the control, whereas AJ18 expression increased significantly. Mineralized nodule formation decreased markedly in the presence of 10(-3) M BA. Alkaline phosphatase activity and the expression of bone sialoprotein and osteocalcin decreased significantly in the presence of 10(-3) M BA compared to the control. These results suggest that 10(-3) M BA suppresses osteoblastic differentiation and mineralized nodule formation in ROS17/2.8 cells.
authors.affiliation: Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan. morotan@rio.odn.ne.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Morozumi
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D015153:Blotting, Western; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis / Q000235:genetics; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000096:biosynthesis* / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D016335:Zinc Fingers / Q000187:drug effects
chemical_list: D002087:Butyrates; D050659:Core Binding Factor Alpha 1 Subunit; C088334:Distal-less homeobox proteins; D016326:Extracellular Matrix Proteins; D018398:Homeodomain Proteins; D058574:Integrin-Binding Sialoprotein; C084119:MSX2 protein; D012097:Repressor Proteins; C495181:Sp7 protein, rat; D014157:Transcription Factors; C485806:Zfp354c protein, rat; D015675:Osteocalcin; D000469:Alkaline Phosphatase