title: The current status of alternatives to animal testing and predictive toxicology methods using liver microfluidic biochips.
abstract: In this paper, we will consider new in vitro cell culture platforms and the progress made, based on the microfluidic liver biochips dedicated to pharmacological and toxicological studies. Particular emphasis will be given to recent developments in the microfluidic tools dedicated to cell culture (more particularly liver cell culture), in silico opportunities for Physiologically Based PharmacoKinetic (PBPK) modelling, the challenge of the mechanistic interpretations offered by the approaches resulting from "multi-omics" data (transcriptomics, proteomics, metabolomics, cytomics) and imaging microfluidic platforms. Finally, we will discuss the critical features regarding microfabrication, design and materials, and cell functionality as the key points for the future development of new microfluidic liver biochips.
authors.affiliation: CNRS UMR 6600, Laboratoire de Biomécanique et Bioingénierie, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: Jean Matthieu
authors.initials: JM
authors.lastname: Prot
authors.forename: Eric
authors.initials: E
authors.lastname: Leclerc
mesh_terms: D016475:3T3 Cells; D000818:Animals; D004285:Dogs; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D014116:Toxicology / Q000295:instrumentation / Q000379:methods