title: Short communication: Increased expression of secretory leukocyte protease inhibitor in oral mucosa of Colombian HIV type 1-exposed seronegative individuals.
abstract: The exposure to human immunodeficiency virus type 1 (HIV-1) does not always result in infection. Indeed, there are individuals who have been repeatedly exposed to HIV-1 but do not exhibit clinical or serological evidence of infection; they are known as HIV-exposed seronegative individuals (HESN). To determine if secretory leukocyte protease inhibitor (SLPI), a soluble factor secreted by epithelial cells lining mucosal surfaces that showed anti-HIV activity in vitro, was associated with natural resistance to HIV infection, we measured by real time RT-PCR the expression of SLPI in oral mucosa of a cohort of Colombian HESN, in chronically HIV-1-infected individuals and in healthy controls. The HESN expressed significantly higher levels of SLPI mRNA than healthy controls (p=0.033) and chronically infected subjects (p=0.011). These findings suggest an association between SLPI expression and the natural resistance to HIV-1 infection exhibited by our HESN cohort.
authors.affiliation: Immunovirology Group, University of Antioquia, Medellin, Colombia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Taborda
authors.forename: Wildeman
authors.initials: W
authors.lastname: Zapata-Builes
authors.forename: Carlos
authors.initials: C
authors.lastname: Montoya
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Rugeles
mesh_terms: D000293:Adolescent; D000328:Adult; D003105:Colombia; D004306:Dose-Response Relationship, Immunologic; D004848:Epithelium; D005260:Female; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000276:immunology*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D012309:Risk-Taking; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000276:immunology*; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor