title: Synaptic localisation of agmatinase in rat cerebral cortex revealed by virtual pre-embedding.
abstract: Light microscopic evidence suggested a synaptic role for agmatinase, an enzyme capable of inactivating the putative neurotransmitter and endogenous anti-depressant agmatine. Using electron microscopy and an alternative pre-embedding approach referred to as virtual pre-embedding, agmatinase was localised pre- and postsynaptically, to dendritic spines, spine and non-spine terminals, and dendritic profiles. In dendritic spines, labelling displayed a tendency towards the postsynaptic density. These results further strengthen a synaptic role for agmatine and strongly suggest a regulatory role for synaptically expressed agmatinase.
authors.affiliation: Institute for Integrative Neuroanatomy, Charité-Universitätsmedizin Berlin, Philippstrasse 12, Berlin, Germany.
authors.forename: V I
authors.initials: VI
authors.lastname: Madai
authors.forename: W C
authors.initials: WC
authors.lastname: Poller
authors.forename: D
authors.initials: D
authors.lastname: Peters
authors.forename: J
authors.initials: J
authors.lastname: Berger
authors.forename: K
authors.initials: K
authors.lastname: Paliege
authors.forename: R
authors.initials: R
authors.lastname: Bernard
authors.forename: R W
authors.initials: RW
authors.lastname: Veh
authors.forename: G
authors.initials: G
authors.lastname: Laube
mesh_terms: D000818:Animals; D049229:Dendritic Spines / Q000201:enzymology* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry; D010296:Parietal Lobe / Q000201:enzymology* / Q000648:ultrastructure; D016613:Plastic Embedding; D051381:Rats; D012235:Rhodamines / Q000737:chemistry; D013194:Staining and Labeling; D013569:Synapses / Q000201:enzymology* / Q000648:ultrastructure; D014512:Ureohydrolases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; D014512:Ureohydrolases; C019625:agmatinase