title: Production and characterization of biosurfactant from marine Streptomyces species B3.
abstract: The present study demonstrates the production and properties of a biosurfactant isolated from marine Streptomyces species B3. The production of the biosurfactant was found to be higher in medium containing sucrose and lower in the medium containing glycerol. Yeast extract was the best nitrogen source for the production of the biosurfactant. The isolated biosurfactant reduced the surface tension of water to 29 mN/m. The purified biosurfactant was shown critical micelle concentrations of 110 mg/l. The emulsifying activity and stability of the biosurfactant was investigated at different salinities, pH, and temperature. The biosurfactant was effective at very low concentrations over a wide range of temperature, pH, and salt concentration. The purified biosurfactant was shown strong antimicrobial activity. The biosurfactant was produced from the marine Streptomyces sp. using non-hydrocarbon substrates such as sucrose that was readily available and not required extensive purification procedure. Streptomyces species B3 can be used for microbially enhanced oil recovery process.
authors.affiliation: Department of Pharmaceutical Biotechnology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune 411 038, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Khopade
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Liu
authors.forename: Kakasaheb
authors.initials: K
authors.lastname: Mahadik
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Kokare
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D008823:Micelles; D013302:Streptomyces / Q000737:chemistry*; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D008823:Micelles; D013501:Surface-Active Agents