title: Repair of rabbit femur defects with organic bovine bone cancellous block or cortical granules.
abstract: OBJECTIVE
  To investigate the osseous repair in epiphyseal rabbit femur defects treated with organic bovine bone (OBB) cancellous block or OBB cortical granules.
  
  
  METHODS
  Forty-eight trephined defects were made bilaterally in 30 rabbits. In 18 animals, the defects were filled with OBB cancellous block (experimental group 1) or OBB cortical granules (experimental group 2), and in 12 animals (control group), the defects on both sides were filled only with blood clot. After 30, 90, and 180 days, the femur epiphyses were collected, fixed, radiographed both digitally and conventionally, and then processed for histologic analyses. The newly formed cortical bone was measured in the histologic sections using a digital image analysis system.
  
  
  RESULTS
  Histologically, both experimental groups showed total or partial closure of the defects with woven bone at 30 days, whereas in the control sites, a marked migration of connective tissue into the defect was apparent. At 90 and 180 days, a similar pattern of repair was observed in the experimental groups and the control group. Quantitatively, however, no significant differences between groups (P > .05) were observed throughout the experimental period. A significant reduction in newly formed cortical bone occurred at 90 and 180 days (P < .05) in all groups. Radiographically, no statistically significant differences were seen among the groups (P > .05).
  
  
  CONCLUSIONS
  The study showed minor descriptive differences at the first healing period that were not evident at later periods and that were not shown through histomorphometry and radiographic analysis. The only significant results were related to time.
authors.affiliation: Bauru Dental School, University of São Paulo, Brazil. lucianavb@uol.com.br
authors.forename: Luciana Viti
authors.initials: LV
authors.lastname: Betti
authors.forename: Clovis Monteiro
authors.initials: CM
authors.lastname: Bramante
authors.forename: Tânia Mary
authors.initials: TM
authors.lastname: Cestari
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.forename: Roberto Brandão
authors.initials: RB
authors.lastname: Garcia
authors.forename: Ivaldo Gomes
authors.initials: IG
authors.lastname: de Moraes
authors.forename: Norberti
authors.initials: N
authors.lastname: Bernardineli
authors.forename: Rumio
authors.initials: R
authors.lastname: Taga
mesh_terms: D000818:Animals; D001857:Bone Matrix; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000008:administration & dosage*; D002417:Cattle; D005269:Femur / Q000187:drug effects / Q000601:surgery; D008297:Male; D008903:Minerals / Q000008:administration & dosage*; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits
chemical_list: D018786:Bone Substitutes; D008903:Minerals