title: Determination of blood volume by pulse CO-oximetry.
abstract: The objective of this study was to determine whether changes in carboxyhaemoglobin (COHb) saturation following carbon monoxide (CO) rebreathing can be accurately detected by pulse CO-oximetry in order to determine blood volume. Noninvasive measurements of carboxyhaemoglobin saturation (SpCO) were continuously monitored by pulse CO-oximetry before, during and following 2 min of CO rebreathing. Reproducibility and accuracy of noninvasive blood volume measurements were determined in 16 healthy non-smoking individuals (15 males, age: 28 ± 2 years, body mass index: 25.4 ± 0.6 kg m(-2)) through comparison with blood volume measurements calculated from invasive measurements of COHb saturation. The coefficient of variation for noninvasive blood volume measurements performed on separate days was 15.1% which decreases to 9.1% when measurements were performed on the same day. Changes in COHb saturation and SpCO following CO rebreathing were strongly correlated (r = 0.90, p < 0.01), resulting in a significant correlation between invasive and noninvasive blood volume measurements (r = 0.83, p = 0.02). Changes in SpCO following CO rebreathing can be accurately detected by pulse CO-oximetry, which could potentially provide a simplified, convenient and reproducible method to rapidly determine blood volume in healthy individuals.
authors.affiliation: Division of Cardiovascular Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA. lalande.sophie@mayo.edu
authors.forename: S
authors.initials: S
authors.lastname: Lalande
authors.forename: J W
authors.initials: JW
authors.lastname: Kelsey
authors.forename: M J
authors.initials: MJ
authors.lastname: Joyner
authors.forename: B D
authors.initials: BD
authors.lastname: Johnson
mesh_terms: D000328:Adult; D001784:Blood Gas Analysis / Q000379:methods; D001810:Blood Volume; D001811:Blood Volume Determination / Q000379:methods; D002248:Carbon Monoxide / Q000097:blood*; D002263:Carboxyhemoglobin / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D010092:Oximetry / Q000379:methods*; D055815:Young Adult
chemical_list: D002248:Carbon Monoxide; D002263:Carboxyhemoglobin