title: A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall             survival time in cholangiocarcinoma.
abstract: Trefoil factor 2 (TFF2) is a member of trefoil factor family found to be             overexpressed in many cancers including cholangiocarcinoma (CCA). The majority             of studies have focused on wild-type TFF2 (wtTFF2) expression, but information             regarding alternative splicing variants of TFF2 mRNA has not been reported. In             this study, we aimed to identify and quantify a novel TFF2 splice variant in cholangiocarcinoma             (CCA). Seventy-eight tumors and 15 normal adjacent tissues were quantified for             the expression of the TFF2 splice variant relative to wild-type (wt) TFF2 mRNA             using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR).             The ratio of TFF2 splice variant against wtTFF2 was analyzed for associations             with clinical parameters. We found a novel TFF2 splice variant, exon 2 skipping             (∆EX2TFF2), resulting in a stop codon (TAG) at exon 1. The ∆EX2TFF2/wtTFF2 ratio             in tumors was significantly higher than in normal tissue (P<0.01). Interestingly,             high ∆EX2TFF2/wtTFF2 ratio was significantly associated with good prognosis compared             with low ratio (P=0.017). In contrast, the presence of wtTFF2 protein was associated             with poor survival of CCA patients (P=0.034). This is the first report of a trefoil             factor splice variant and its potential application as a prognostic biomarker             in CCA.
authors.affiliation: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Surasee
authors.initials: S
authors.lastname: Kamlua
authors.forename: Siriporn
authors.initials: S
authors.lastname: Patrakitkomjorn
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.forename: Trevelyan R
authors.initials: TR
authors.lastname: Menheniott
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Giraud
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D016009:Chi-Square Distribution; D018281:Cholangiocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D018570:Risk Assessment; D012307:Risk Factors; D013785:Thailand; D013997:Time Factors; D000071164:Trefoil Factor-2
chemical_list: D014408:Biomarkers, Tumor; D010455:Peptides; D020033:Protein Isoforms; D012333:RNA, Messenger; C000604007:TFF2 protein, human; D000071164:Trefoil Factor-2