title: High-throughput sequencing of RNA silencing-associated small RNAs in olive (Olea europaea L.).
abstract: Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA) regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive.
authors.affiliation: Department of Environmental Biology, Centro de Investigaciones Biológicas, The Spanish National Research Council, Madrid, Spain.
authors.forename: Livia
authors.initials: L
authors.lastname: Donaire
authors.forename: Laia
authors.initials: L
authors.lastname: Pedrola
authors.forename: Raúl de la
authors.initials: Rde L
authors.lastname: Rosa
authors.forename: César
authors.initials: C
authors.lastname: Llave
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D031658:Olea / Q000235:genetics* / Q000254:growth & development; D011110:Polymorphism, Genetic; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013997:Time Factors
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant