title: Magnetic assistance highly sensitive protein assay based on surface-enhanced resonance Raman scattering.
abstract: A simple and effective surface-enhanced Raman scattering (SERS)-based protocol for the detection of protein-small molecule interactions has been developed. We employed silver-coated magnetic particles (AgMNPs), which can provide high SERS activity as a protein carrier to capture a small molecule. Combining magnetic separation and the SERS method for protein detection, highly reproducible SERS spectra of a protein-small molecule complex can be obtained with high sensitivity. This time-saving method employs an external magnetic field to induce the AgMNPs to aggregate to increase the amount of atto610-biotin/avidin complex in a unit area with the SERS enhancement. Because of the contribution of the AgMNP aggregation to the SERS, this protocol has great potential for practical high-throughput detection of the protein-small molecule complex and the antigen-antibody immunocomplex.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Wonjin
authors.initials: W
authors.lastname: Hong
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Guo
authors.forename: Youngjo
authors.initials: Y
authors.lastname: Sa
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Jung
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002417:Cattle; D060526:Magnetic Fields; D011506:Proteins; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D014732:Vibration
chemical_list: D011506:Proteins; D012710:Serum Albumin, Bovine; D012834:Silver