title: Alterations in the expression of translation factors as molecular markers in cadmium-exposed workers.
abstract: Eukaryotic translation initiation factor 3 (eIF3) and elongation factor 1δ (eEF-1δ) are novel cadmium (Cd) responsive proto-oncogenes. This research investigated the expression of these genes in Cd-exposed workers (n = 58), and to evaluate their usefulness as biomarkers of Cd exposure. According to urinary Cd concentration, the subjects were divided into four groups (urinary Cd concentration ≥0.1 μg/g.Cr, ≥1.0 μg/g.Cr, ≥5.0 μg/g.Cr and ≥50.0 μg/g.Cr). Subjects exhibited increased severe health problems with higher urinary Cd concentrations. The eIF3 and eEF-1δ expression in the blood were investigated with real-time PCR. PCR data showed a strong positive correlation between blood eEF-1δ and urinary Cd concentrations (r = 0.788, p < 0.01), and a weak positive correlation between blood eIF3 expression and urinary Cd concentrations (r = 0.569, p < 0.05). These findings, for the first time, demonstrate that the blood eEF-1δ overexpression can be used as a molecular biomarker of Cd-exposed population.
authors.affiliation: School of Public Health, Guangzhou Medical University, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Wei
authors.forename: Yi-xiong
authors.initials: YX
authors.lastname: Lei
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.forename: Chao-cai
authors.initials: CC
authors.lastname: He
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002104:Cadmium / Q000633:toxicity* / Q000652:urine*; D002681:China; D039621:Eukaryotic Initiation Factor-3 / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D009784:Occupational Diseases / Q000097:blood / Q000139:chemically induced* / Q000652:urine; D016273:Occupational Exposure; D020648:Peptide Elongation Factor 1 / Q000097:blood*; D010612:Pharyngitis / Q000097:blood / Q000139:chemically induced* / Q000652:urine
chemical_list: D015415:Biomarkers; D039621:Eukaryotic Initiation Factor-3; D020648:Peptide Elongation Factor 1; D002104:Cadmium