title: Effects of decorin and biglycan on human airway smooth muscle cell adhesion.
abstract: Growth on a decorin matrix results in decreased human airway smooth muscle cell (HASMC) number, by decreasing proliferation and increasing apoptosis. We questioned whether these effects were related to abnormal extracellular matrix (ECM)-cell adhesion. HASMCs were seeded on decorin, biglycan, collagen type I or plastic. Actin organization and focal adhesion formation were assessed by staining for filamentous (F) and globular (G) actin, and vinculin, respectively. Gene expression for focal adhesion proteins, ECM molecules and HASMC receptors was measured. Protein levels for fibronectin, α(2), α(5), α(v) and β(3) integrin subunits and, focal adhesion kinase (FAK) were assessed. F-actin filaments were prominent in cells seeded on collagen I and plastic, less apparent in cells cultured on biglycan and faint in cells on decorin. Vinculin clustering was decreased in cells seeded on decorin and biglycan, as was vinculin gene expression. Compared to cells on plastic, cells on decorin had an increase in fibronectin gene expression. Seeding on decorin caused an increase in α(2) integrin subunit and platelet-derived growth factor receptor A gene expression. There was also an increase in α(2) and α(v) integrin subunit protein. Finally, FAK protein levels in cells seeded on decorin or biglycan were decreased compared to cells seeded on plastic or collagen I. Cells grown on proteoglycan matrices demonstrate evidence of abnormalities during many of the key processes involved in normal cell adhesion. Upregulation of cell surface receptor proteins, such as α(2) integrin subunit, may represent a compensatory mechanism to overcome poor adhesion induced by growth on these matrices.
authors.affiliation: Meakins Christie Laboratories, McGill University, Montreal, Quebec, Canada.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: D'Antoni
authors.forename: Paul-André
authors.initials: PA
authors.lastname: Risse
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferraro
authors.forename: James G
authors.initials: JG
authors.lastname: Martin
authors.forename: Mara S
authors.initials: MS
authors.lastname: Ludwig
mesh_terms: D008841:Actin Cytoskeleton; D000199:Actins / Q000235:genetics / Q000378:metabolism; D058578:Biglycan / Q000494:pharmacology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D058575:Decorin / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism; D039564:Integrin alphaV / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010969:Plastics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D012137:Respiratory System / Q000166:cytology / Q000378:metabolism; D013194:Staining and Labeling; D016596:Vinculin / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D058578:Biglycan; D024042:Collagen Type I; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039421:Integrin alpha2; D039564:Integrin alphaV; D010969:Plastics; C488955:VCL protein, human; D016596:Vinculin; D020796:Receptor, Platelet-Derived Growth Factor alpha; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human