title: UM-SCC-104: a new human papillomavirus-16-positive cancer stem cell-containing head and neck squamous cell carcinoma cell line.
abstract: BACKGROUND
  Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs).
  
  
  METHODS
  Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods.
  
  
  RESULTS
  UM-SCC-104 has a unique genotype, contains HPV-16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(-) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(-) cells did not produce tumors.
  
  
  CONCLUSIONS
  UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs.
authors.affiliation: Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: Alice L
authors.initials: AL
authors.lastname: Tang
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Hauff
authors.forename: John H
authors.initials: JH
authors.lastname: Owen
authors.forename: Martin P
authors.initials: MP
authors.lastname: Graham
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Czerwinski
authors.forename: Jung Je
authors.initials: JJ
authors.lastname: Park
authors.forename: Heather
authors.initials: H
authors.lastname: Walline
authors.forename: Silvana
authors.initials: S
authors.lastname: Papagerakis
authors.forename: Jay
authors.initials: J
authors.lastname: Stoerker
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: McHugh
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Chepeha
authors.forename: Carol R
authors.initials: CR
authors.lastname: Bradford
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Carey
authors.forename: Mark E
authors.initials: ME
authors.lastname: Prince
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology; D012367:RNA, Viral / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D000077195:Squamous Cell Carcinoma of Head and Neck; D014183:Transplantation, Heterologous
chemical_list: D012367:RNA, Viral