title: The effect of royal sun agaricus, Agaricus brasiliensis S. Wasser et al., extract on methyl methanesulfonate caused genotoxicity in Drosophila melanogaster.
abstract: The effect of culinary-medicinal Royal Sun Agaricus (Agaricus brasiliensis) hot water extract on methyl methane sulfonate (MMS) induced mutagenicity/genotoxity in Drosophila melanogaster was studied using a quick and broadly applicable in vivo assay, i.e., the wing somatic mutation and recombination test. We used 2nd instar larvae, trans-heterozygous for the third chromosome recessive markers, i.e., multiple wing hairs (mvh) and flare-3 [flr (3)], and fed them for 24 h with the aqueous extract of A. brasiliensis. For antigenotoxicity studies a 24-h pretreatment with the extract was done, followed by a 48-h treatment of the then 3rd instar larvae with MMS. The frequency of mutations of the wing blade changes (i.e., of the number of wing spots of different sizes) induced in somatic cells was determined as a parameter of genetic changes of the wing imaginal discs. The results showed that A. brasiliensis extract did not cause any genotoxic or mutagenic effects. No antigenotoxic and/or protective effect against the induction of mutations by MMS was observed. Instead, a possible enhanced mitotic recombination frequency by MMS was seen after pretreatment of the larvae with A. brasiliensis extract. Possible mechanisms of action are discussed.
authors.affiliation: Institute of Biological Research, University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade, Serbia. tanjat@ibiss.bg.ac.rs
authors.forename: Tatjana
authors.initials: T
authors.lastname: Savić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Patenković
authors.forename: Marina
authors.initials: M
authors.lastname: Soković
authors.forename: Jasmina
authors.initials: J
authors.lastname: Glamoclija
authors.forename: Marko
authors.initials: M
authors.lastname: Andjelković
authors.forename: Leo J L D
authors.initials: LJ
authors.lastname: van Griensven
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D016587:Antimutagenic Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D001685:Biological Factors / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D055951:Functional Food / Q000032:analysis*; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D014921:Wings, Animal / Q000187:drug effects / Q000254:growth & development
chemical_list: D016587:Antimutagenic Agents; D001685:Biological Factors; D009153:Mutagens; D008741:Methyl Methanesulfonate