title: Gene polymorphisms of angiotensin-converting enzyme and angiotensin II type 1 receptor among chronic kidney disease patients in a Chinese population.
abstract: Chronic kidney disease (CKD) is highly prevalent in Taiwan and an increasing number of patients are affected, with a high risk of progression to end-stage renal disease and huge medical expenses. It has been predicted that the presence of hypertension increases with decreasing renal function due to a decrease in sodium excretion and activation of the renin-angiotensin system (RAS). The aim of this study was to investigate the influence of genetic variants of the RAS gene on CKD. We performed a case control association study and genotyped 135 CKD patients and 270 healthy controls among Han Chinese in Taiwan. All subjects were genotyped for angiotensinogen (AGT-M235T, T174M, A-20C), angiotensin-I converting enzyme (ACE-A2350G) and angiotensin II type 1 receptor (AGTR1-A1166C, C573T, C-521T) polymorphisms of RAS genes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Significant associations were observed in ACE-A2350G and AGTR1-C573T polymorphism between CKD patients and controls. In regard to ACE-A2350G, compared with the AA genotype the GG genotype protected against CKD (adjusted odds ratio [OR] = 0.34; p = 0.01). In regard to AGTR1-C573T, the CT genotype was a risk for CKD compared with the CC genotype (adjusted OR = 1.82; p = 0.03). We conclude that ACE-A2350G and AGTR1-C573T polymorphisms are likely candidate determinants of CKD.
authors.affiliation: School of Public Health, National Defense Medical Center, Taipei, Taiwan, ROC. a131419@gmail.com
authors.forename: Sui-Lung
authors.initials: SL
authors.lastname: Su
authors.forename: Kuo-Cheng
authors.initials: KC
authors.lastname: Lu
authors.forename: Yuh-Feng
authors.initials: YF
authors.lastname: Lin
authors.forename: Yu-Juei
authors.initials: YJ
authors.lastname: Hsu
authors.forename: Pong-Ying
authors.initials: PY
authors.lastname: Lee
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Yang
authors.forename: Sen-Yeong
authors.initials: SY
authors.lastname: Kao
mesh_terms: D000808:Angiotensinogen / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D012084:Renin-Angiotensin System / Q000235:genetics
chemical_list: C544065:AGTR1 protein, human; D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A