title: Interaction of human serum albumin with 10-hydroxycamptothecin: spectroscopic and molecular modeling studies.
abstract: In this work, fluorescence spectroscopy in combination with circular dichroism spectroscopy and molecular modeling was employed to investigate the binding of 10-hydroxycamptothecin (HCPT) to human serum albumin (HSA) under simulative physiological conditions. The experiment results showed that the fluorescence quenching of HSA by HCPT was a result of the formation of HCPT-HSA complex. The corresponding association constants (K                         (a)) between HCPT and HSA at four different temperatures were determined according to the modified Stern-Volmer equation. The results of thermodynamic parameters ΔG, ΔH, and ΔS indicated that hydrogen bonds and van der Waals forces played major roles for HCPT-HSA association. Site marker competitive displacement experiment indicated that the binding of HCPT to HSA primarily took place in sub-domain IIA (site I). Molecular docking study further confirmed the binding mode and the binding site obtained by fluorescence and site marker competitive experiments. The conformational investigation showed that the presence of HCPT decreased the α-helical content of HSA and induced the slight unfolding of the polypeptides of protein, which confirmed some micro-environmental and conformational changes of HSA molecules.
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, 434023, Hubei, People's Republic of China.
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ye-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Yang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yuan
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002166:Camptothecin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D012709:Serum Albumin; C028098:10-hydroxycamptothecin; D002166:Camptothecin