title: Magnetic nanoparticles enhance adenovirus transduction in vitro and in vivo.
abstract: OBJECTIVE
  Adenoviruses are among the most powerful gene delivery systems. Even if they present low potential for oncogenesis, there is still a need for minimizing widespread delivery to avoid deleterious reactions. In this study, we investigated Magnetofection efficiency to concentrate and guide vectors for an improved targeted delivery.
  
  
  METHODS
  Magnetic nanoparticles formulations were complexed to a replication defective Adenovirus and were used to transduce cells both in vitro and in vivo. A new integrated magnetic procedure for cell sorting and genetic modification (i-MICST) was also investigated.
  
  
  RESULTS
  Magnetic nanoparticles enhanced viral transduction efficiency and protein expression in a dose-dependent manner. They accelerated the transduction kinetics and allowed non-permissive cells infection. Magnetofection greatly improved adenovirus-mediated DNA delivery in vivo and provided a magnetic targeting. The i-MICST results established the efficiency of magnetic nanoparticles assisted viral transduction within cell sorting columns.
  
  
  CONCLUSIONS
  The results showed that the combination of Magnetofection and Adenoviruses represents a promising strategy for gene therapy. Recently, a new integrated method to combine clinically approved magnetic cell isolation devices and genetic modification was developed. In this study, we validated that magnetic cell separation and adenoviral transduction can be accomplished in one reliable integrated and safe system.
authors.affiliation: OZ Biosciences, Parc Scientifique de Luminy, 163 Avenue de Luminy case 922, 13288 Marseille cedex 9, France. csapet@ozbiosciences.com
authors.forename: Cédric
authors.initials: C
authors.lastname: Sapet
authors.forename: Christophe
authors.initials: C
authors.lastname: Pellegrino
authors.forename: Nicolas
authors.initials: N
authors.lastname: Laurent
authors.forename: Flavie
authors.initials: F
authors.lastname: Sicard
authors.forename: Olivier
authors.initials: O
authors.lastname: Zelphati
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: D049452:Green Fluorescent Proteins