title: Interaction of 5-Fluorouracil and its derivatives with bovine serum albumin.
abstract: Fluorouracil (5-FU) and its derivatives are the most commonly used drugs to treat many types of cancer. Two dual functional agents, FUPAE and FUPAP, derived from 5-Fluorouracil (5-FU) have shown radiosensitizing activity but unlike their components were not cytotoxic. This study was designed to examine the interaction of BSA with 5-Fluorouracil (5-FU) and two of its derivatives; FUPAE and FUPAP at physiological conditions, using a constant protein concentration and various drug contents. FTIR, UV-Vis spectroscopic methods as well as molecular modelling were used to determine the drugs binding mode, the binding constants and the effects of drug complexation on BSA stability and conformation. Structural analysis showed that 5-Fluorouracil, FUPAE and FUPAP bind BSA via polypeptide polar groups with overall binding constants of K(5-FU-BSA)=3.02(±0.09)×10(3), K(FUPAE-BSA)=1.08(±0.04)×10(4), K(FUPAP-BSA)=1.21(±0.06)×10(4) M(-1). BSA conformation was altered by a major reduction of α-helix from 69% (free BSA) to 34% with 5-FU, 40% with FUPAE, 38% with FUPAP. These results suggest that serum albumins might act as carrier proteins for FUPAE and FUPAP in delivering them to target tissues.
authors.affiliation: Department of Medicinal Chemistry and Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Abdi
authors.forename: Sh
authors.initials: Sh
authors.lastname: Nafisi
authors.forename: F
authors.initials: F
authors.lastname: Manouchehri
authors.forename: M
authors.initials: M
authors.lastname: Bonsaii
authors.forename: A
authors.initials: A
authors.lastname: Khalaj
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D055550:Protein Stability / Q000187:drug effects; D017434:Protein Structure, Tertiary / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D012710:Serum Albumin, Bovine; D005472:Fluorouracil