title: MTP -493G/T gene polymorphism is associated with steatosis in hepatitis C-infected patients.
abstract: The reduction of hepatic microsomal transfer protein (MTP) activity results in fatty liver, worsening hepatic steatosis and fibrosis in chronic hepatitis C (CHC). The G allele of the MTP gene promoter, -493G/T, has been associated with lower transcriptional activity than the T allele. We investigated this association with metabolic and histological variables in patients with CHC. A total of 174 untreated patients with CHC were genotyped for MTP -493G/T by direct sequencing using PCR. All patients were negative for markers of Wilson's disease, hemochromatosis and autoimmune diseases and had current and past daily alcohol intake lower than 100 g/week. The sample distribution was in Hardy-Weinberg equilibrium. Among subjects with genotype 1, 56.8% of the patients with fibrosis grade 3+4 presented at least one G allele versus 34.3% of the patients with fibrosis grade 1+2 (OR = 1.8; 95%CI = 1.3-2.3). Logistic regression analysis with steatosis as the dependent variable identified genotypes GG+GT as independent protective factors against steatosis (OR = 0.4, 95%CI = 0.2-0.8; P = 0.01). The results suggest that the presence of the G allele of MTP -493G/T associated with lower hepatic MTP expression protects against steatosis in our CHC patients.
authors.affiliation: Departamento de Gastroenterologia, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: E R F
authors.initials: ER
authors.lastname: Siqueira
authors.forename: C P M S
authors.initials: CP
authors.lastname: Oliveira
authors.forename: M L
authors.initials: ML
authors.lastname: Correa-Giannella
authors.forename: J T
authors.initials: JT
authors.lastname: Stefano
authors.forename: A M
authors.initials: AM
authors.lastname: Cavaleiro
authors.forename: M A H Z
authors.initials: MA
authors.lastname: Fortes
authors.forename: M T C
authors.initials: MT
authors.lastname: Muniz
authors.forename: F S
authors.initials: FS
authors.lastname: Silva
authors.forename: L M M B
authors.initials: LM
authors.lastname: Pereira
authors.forename: F J
authors.initials: FJ
authors.lastname: Carrilho
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000235:genetics*; D018450:Disease Progression; D005234:Fatty Liver / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D019698:Hepatitis C, Chronic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; C106092:microsomal triglyceride transfer protein