title: [Detection of urine antibodies to Schistosoma japonicum by magnetic particle affinity immunoassay].
abstract: OBJECTIVE
  To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum.
  
  
  METHODS
  The urine antibodies to S. japonicum were detected by magnetic particle affinity immunoassay (MPAIA) in 158 cases of schistosomiasis japonica and 100 health persons, and their serum antibodies to S. japonicum were also detected at the same time.
  
  
  RESULTS
  The sample of urine by MPAIA was 10 microl original urine without any special treatment. The positive rate of urine and serum were 48.10% (76/158)and 88.61% (140/158), respectively. There was difference between the performance of two methods (chi2 = 60.24, P < 0.05). However, both of their specificity were 100% (100/100).
  
  
  CONCLUSIONS
  MPAIA is viable for detection of urine antibodies to S. japonicum, but its sensitivity should be improved.
authors.affiliation: Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Chao-Wei
authors.initials: CW
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Lan-Ying
authors.initials: LY
authors.lastname: Wei
authors.forename: Chang-Fu
authors.initials: CF
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000652:urine; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008280:Magnetics / Q000379:methods*; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth