title: Metaphase FISH on a chip: miniaturized microfluidic device for fluorescence in situ hybridization.
abstract: Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3 5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.
authors.affiliation: DTU-Nanotech, Department of Micro and Nanotechnology, Technical University of Denmark, Kgs Lyngby 2800, Denmark. Indumathi.Vedarethinam@nanotech.dtu.dk
authors.forename: Indumathi
authors.initials: I
authors.lastname: Vedarethinam
authors.forename: Pranjul
authors.initials: P
authors.lastname: Shah
authors.forename: Maria
authors.initials: M
authors.lastname: Dimaki
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Tumer
authors.forename: Niels
authors.initials: N
authors.lastname: Tommerup
authors.forename: Winnie E
authors.initials: WE
authors.lastname: Svendsen
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008677:Metaphase / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D014178:Translocation, Genetic / Q000235:genetics