title: Inactivation of MYO5B promotes invasion and motility in gastric cancer cells.
abstract: BACKGROUND
  Loss of cell polarity and tissue disorganisation are hallmarks of cancer. MYO5B mutations disrupt epithelial cell polarity, suggesting that MYO5B may be involved in tumorigenesis.
  
  
  METHODS
  We analyzed MYO5B expression in 70 gastric cancer tissues by immunohistochemistry using a tissue microarray method. Two related proteins, Rab11a and TfR, were also investigated.
  
  
  RESULTS
  We found that the negative rate of MYO5B was 78.6 and 17.1% in gastric cancer and normal gastric tissues (P < 0.001), respectively. The MYO5B expression had a strong relationship with Rab11a expression (P = 0.002). We also found that inactivation by siRNA against MYO5B promoted the proliferation, invasion and migration of gastric cancer cells.
  
  
  CONCLUSIONS
  The expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis. Therefore, MYO5B may become an important biomarker for gastric cancer in the future.
authors.affiliation: Department of Internal Medicine-Oncology, The First Affiliated Hospital, Zhengzhou University, 1 Western Jianshe Road, Zhengzhou 450052, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Dong
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Dongli
authors.initials: D
authors.lastname: Yue
authors.forename: Linan
authors.initials: L
authors.lastname: Zhu
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Fan
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D016764:Cell Polarity; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009154:Mutation; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D024701:Myosin Type V / Q000235:genetics / Q000378:metabolism; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009367:Neoplasm Staging; D034741:RNA, Small Interfering / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020691:rab GTP-Binding Proteins / Q000378:metabolism; D000099193:rab11 GTP-Binding Proteins
chemical_list: D015703:Antigens, CD; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D034741:RNA, Small Interfering; D011990:Receptors, Transferrin; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C068126:CD71 antigen; C505832:MYO5B protein, human