title: In vitro study of microleakage of fissure sealant with different previous treatments.
abstract: The purpose of this work was to evaluate microleakeage of a sealant after using three different techniques for conditioning the surface to be sealed. Twenty-four caries-free upper and lower premolars were used, which were preserved in distilled water at room temperature. The structural faults were enlarged using a cylindrical conical diamond (ISO 007). Teeth were randomly assigned into three groups of eight. Group I (control) was conditioned with 37% phosphoric acid (Vivadent) for 15 seconds after which the sealant Helioseal F (Vivadent) was applied and cured for 40 seconds. Group II was conditioned in the same way, after which one-step adhesive Te-econom (Vivadent) and the sealant were applied. Group III was conditioned using a self-etching adhesive, Go (SDI), after which the sealant was applied. Adhesive was applied according to the manufacturer's instructions. The samples were thermocycled for 300 cycles between 5 degrees and 55 degreesC and immersed in a 2% methylene blue solution for 48 hs. at standardized temperature of 37 degreesC +/- 1 degree. Then they were rinsed with tap water and ground longitudinally in V-P direction with silica carbide rotatory disks of decreasing grit. The amount of leakage was evaluated under stereoscopic microscope at 40X magnification. The longitudinal penetration of dye into the tooth-sealant interface was scored on a scale of 0 to 3. The results were analyzed by a Kruskal-Wallis non-parametric test. In Group II, 100% of the samples showed low (50%) or no (50%) leakage. Both the other groups had a higher percentage of specimens with high leakage (scores 2 and 3) (P = 0.000). Group II had the best performance, with significant differences (P = 0.0028) compared to the other experimental groups. Marginal leakage was lowest when the tooth was conditioned with phosphoric acid and subsequent application of an adhesive, prior to sealant.
authors.affiliation: Department of Operative Dentistry, Faculty of Dentistry, University of Córdoba, Argentina. urquiamorales@arnet.com.ar
authors.forename: Morales María C
authors.initials: MM
authors.lastname: del Urquía
authors.forename: N
authors.initials: N
authors.lastname: Brasca
authors.forename: M
authors.initials: M
authors.lastname: Girardi
authors.forename: C
authors.initials: C
authors.lastname: Bonnin
authors.forename: M
authors.initials: M
authors.lastname: Ríos
mesh_terms: D003763:Dental Leakage / Q000453:epidemiology; D006801:Humans; D066298:In Vitro Techniques; D010895:Pit and Fissure Sealants
chemical_list: D010895:Pit and Fissure Sealants