title: Can Platelet-rich Plasma Be Used for Skin Rejuvenation? Evaluation of Effects of Platelet-rich Plasma on Human Dermal Fibroblast.
abstract: BACKGROUND
  Autologous platelet-rich plasma has attracted attention in various medical fields recently, including orthopedic, plastic, and dental surgeries and dermatology for its wound healing ability. Further, it has been used clinically in mesotherapy for skin rejuvenation.
  
  
  OBJECTIVE
  In this study, the effects of activated platelet-rich plasma (aPRP) and activated platelet-poor plasma (aPPP) have been investigated on the remodelling of the extracellular matrix, a process that requires activation of dermal fibroblasts, which is essential for rejuvenation of aged skin.
  
  
  METHODS
  Platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared using a double-spin method and then activated with thrombin and calcium chloride. The proliferative effects of aPRP and aPPP were measured by [(3)H]thymidine incorporation assay, and their effects on matrix protein synthesis were assessed by quantifying levels of procollagen type I carboxy-terminal peptide (PIP) by enzyme-linked immunosorbent assay (ELISA). The production of collagen and matrix metalloproteinases (MMP) was studied by Western blotting and reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  Platelet numbers in PRP increased to 9.4-fold over baseline values. aPRP and aPPP both stimulated cell proliferation, with peak proliferation occurring in cells grown in 5% aPRP. Levels of PIP were highest in cells grown in the presence of 5% aPRP. Additionally, aPRP and aPPP increased the expression of type I collagen, MMP-1 protein, and mRNA in human dermal fibroblasts.
  
  
  CONCLUSIONS
  aPRP and aPPP promote tissue remodelling in aged skin and may be used as adjuvant treatment to lasers for skin rejuvenation in cosmetic dermatology.
authors.affiliation: Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Dae Hun
authors.initials: DH
authors.lastname: Kim
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Je
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jeung Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Lee