title: Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds.
abstract: A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g(-1)), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhuotong
authors.initials: Z
authors.lastname: Zeng
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Ya
authors.initials: Y
authors.lastname: Pang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lei
authors.forename: Mengshi
authors.initials: M
authors.lastname: Wu
authors.forename: Pinyun
authors.initials: P
authors.lastname: Ren
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Gengxin
authors.initials: G
authors.lastname: Xie
mesh_terms: D000327:Adsorption / Q000187:drug effects; D001673:Biodegradation, Environmental / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology*; D002733:Chlorophenols / Q000302:isolation & purification; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D042845:Laccase / Q000378:metabolism*; D008280:Magnetics; D009584:Nitrogen / Q000737:chemistry; D010636:Phenols / Q000302:isolation & purification*; D016062:Porosity / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D013696:Temperature; D055454:Trametes / Q000187:drug effects / Q000201:enzymology
chemical_list: D002733:Chlorophenols; D004800:Enzymes, Immobilized; D010636:Phenols; C029107:4-chlorophenol; D002244:Carbon; D042845:Laccase; D009584:Nitrogen