title: Identification of a protein network interacting with TdRF1, a wheat RING ubiquitin ligase with a protective role against cellular dehydration.
abstract: Plants exploit ubiquitination to modulate the proteome with the final aim to ensure environmental adaptation and developmental plasticity. Ubiquitination targets are specifically driven to degradation through the action of E3 ubiquitin ligases. Genetic analyses have indicated wide functions of ubiquitination in plant life; nevertheless, despite the large number of predicted E3s, only a few of them have been characterized so far, and only a few ubiquitination targets are known. In this work, we characterized durum wheat (Triticum durum) RING Finger1 (TdRF1) as a durum wheat nuclear ubiquitin ligase. Moreover, its barley (Hordeum vulgare) homolog was shown to protect cells from dehydration stress. A protein network interacting with TdRF1 has been defined. The transcription factor WHEAT BEL1-TYPE HOMEODOMAIN1 (WBLH1) was degraded in a TdRF1-dependent manner through the 26S proteasome in vivo, the mitogen-activated protein kinase TdWNK5 [for Triticum durum WITH NO LYSINE (K)5] was able to phosphorylate TdRF1 in vitro, and the RING-finger protein WHEAT VIVIPAROUS-INTERACTING PROTEIN2 (WVIP2) was shown to have a strong E3 ligase activity. The genes coding for the TdRF1 interactors were all responsive to cold and/or dehydration stress, and a negative regulative function in dehydration tolerance was observed for the barley homolog of WVIP2. A role in the control of plant development was previously known, or predictable based on homology, for wheat BEL1-type homeodomain1(WBLH1). Thus, TdRF1 E3 ligase might act regulating the response to abiotic stress and remodeling plant development in response to environmental constraints.
authors.affiliation: Agricultural Research Council, Genomics Research Centre, 29017 Fiorenzuola d'Arda, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Guerra
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Mastrangelo
authors.forename: Gema
authors.initials: G
authors.lastname: Lopez-Torrejon
authors.forename: Stephan
authors.initials: S
authors.lastname: Marzin
authors.forename: Patrick
authors.initials: P
authors.lastname: Schweizer
authors.forename: Antonio Michele
authors.initials: AM
authors.lastname: Stanca
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: del Pozo
authors.forename: Luigi
authors.initials: L
authors.lastname: Cattivelli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Mazzucotelli
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D014908:Triticum / Q000166:cytology / Q000201:enzymology*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases