title: Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats.
abstract: The aim of the present study was to investigate the effect of curcumin (Cur) on the activity of ectonucleoside triphosphate diphosphohydrolase (CD39), 5'-nucleotidase (CD73) and adenosine deaminase in platelets of cigarette smoke-exposed rats. For that purpose, we subjected male Wistar rats to a treatment with Cur and cigarette smoke, once a day, 5 days each week, for 30 days. The rats were treated by gavage with Cur or corn oil and then exposed to cigarette smoke. The experimental procedures were divided into two sets of experiments. In the first, the animals were divided into four groups: vehicle (corn oil) or Cur 12·5, 25 or 50 mg·kg(-1) . In the second, the animals were divided into five groups: vehicle (corn oil), smoke, or smoke and Cur 12·5, 25 or 50 mg·kg(-1) . The results showed that treatment with Cur significantly prevented the increased adenosine triphosphate (ATP) (121%) and adenosine monophosphate (AMP) (159%) and the decreased adenosine diphosphate (ADP) (51%) hydrolysis observed in the cigarette smoke-exposed rats Our results suggest that those purinergic enzyme alterations observed in the cigarette smoke-exposed rats could be related to an excessive platelet aggregation and point toward the potential of Cur to modulate purinergic signalling and, consequently, regulate the thrombus formation.
authors.affiliation: Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, RS, Brazil.
authors.forename: Jeandre Augusto
authors.initials: JA
authors.lastname: dos Santos Jaques
authors.forename: Jader Betsch
authors.initials: JB
authors.lastname: Ruchel
authors.forename: Karine Bizzi
authors.initials: KB
authors.lastname: Schlemmer
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Margarete
authors.initials: M
authors.lastname: Bagatini
authors.forename: Viviane do Carmo Gonçalves
authors.initials: Vdo C
authors.lastname: Souza
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Moretto
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Daniela Bitencourt Rosa
authors.initials: DB
authors.lastname: Leal
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001081:Apyrase / Q000235:genetics / Q000378:metabolism*; D001792:Blood Platelets / Q000201:enzymology* / Q000378:metabolism; D030024:Curcuma; D003474:Curcumin / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects
chemical_list: D000227:Adenine Nucleotides; D015703:Antigens, CD; D010936:Plant Extracts; C012596:turmeric extract; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen; D003474:Curcumin