title: Comparative microarray analysis of intestinal lymphocytes following Eimeria acervulina, E. maxima, or E. tenella infection in the chicken.
abstract: Relative expression levels of immune- and non-immune-related mRNAs in chicken intestinal intraepithelial lymphocytes experimentally infected with Eimeria acervulina, E. maxima, or E. tenella were measured using a 10K cDNA microarray. Based on a cutoff of >2.0-fold differential expression compared with uninfected controls, relatively equal numbers of transcripts were altered by the three Eimeria infections at 1, 2, and 3 days post-primary infection. By contrast, E. tenella elicited the greatest number of altered transcripts at 4, 5, and 6 days post-primary infection, and at all time points following secondary infection. When analyzed on the basis of up- or down-regulated transcript levels over the entire 6 day infection periods, approximately equal numbers of up-regulated transcripts were detected following E. tenella primary (1,469) and secondary (1,459) infections, with a greater number of down-regulated mRNAs following secondary (1,063) vs. primary (890) infection. On the contrary, relatively few mRNA were modulated following primary infection with E. acervulina (35 up, 160 down) or E. maxima (65 up, 148 down) compared with secondary infection (E. acervulina, 1,142 up, 1,289 down; E. maxima, 368 up, 1,349 down). With all three coccidia, biological pathway analysis identified the altered transcripts as belonging to the categories of "Disease and Disorder" and "Physiological System Development and Function". Sixteen intracellular signaling pathways were identified from the differentially expressed transcripts following Eimeria infection, with the greatest significance observed following E. acervulina infection. Taken together, this new information will expand our understanding of host-pathogen interactions in avian coccidiosis and contribute to the development of novel disease control strategies.
authors.affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America.
authors.forename: Duk Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Lillehoj
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
authors.forename: Chul Hong
authors.initials: CH
authors.lastname: Kim
authors.forename: Myeong Seon
authors.initials: MS
authors.lastname: Park
authors.forename: Yeong Ho
authors.initials: YH
authors.lastname: Hong
authors.forename: Erik P
authors.initials: EP
authors.lastname: Lillehoj
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000469:parasitology; D003048:Coccidiosis / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks / Q000235:genetics; D007422:Intestines / Q000473:pathology*; D008214:Lymphocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011201:Poultry Diseases / Q000235:genetics* / Q000469:parasitology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
chemical_list: D012333:RNA, Messenger