title: Quartz crystal microbalance detection of DNA single-base mutation based on monobase-coded cadmium tellurium nanoprobe.
abstract: A new method for the detection of point mutation in DNA based on the monobase-coded cadmium tellurium nanoprobes and the quartz crystal microbalance (QCM) technique was reported. A point mutation (single-base, adenine, thymine, cytosine, and guanine, namely, A, T, C and G, mutation in DNA strand, respectively) DNA QCM sensor was fabricated by immobilizing single-base mutation DNA modified magnetic beads onto the electrode surface with an external magnetic field near the electrode. The DNA-modified magnetic beads were obtained from the biotin-avidin affinity reaction of biotinylated DNA and streptavidin-functionalized core/shell Fe(3)O(4)/Au magnetic nanoparticles, followed by a DNA hybridization reaction. Single-base coded CdTe nanoprobes (A-CdTe, T-CdTe, C-CdTe and G-CdTe, respectively) were used as the detection probes. The mutation site in DNA was distinguished by detecting the decreases of the resonance frequency of the piezoelectric quartz crystal when the coded nanoprobe was added to the test system. This proposed detection strategy for point mutation in DNA is proved to be sensitive, simple, repeatable and low-cost, consequently, it has a great potential for single nucleotide polymorphism (SNP) detection.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fanbo
authors.initials: F
authors.lastname: Lin
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Zeng
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D019187:Cadmium Compounds / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D015335:Molecular Probes; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011791:Quartz; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D019187:Cadmium Compounds; D017931:DNA Primers; D015335:Molecular Probes; D011791:Quartz; D004247:DNA; D013691:Tellurium; C028337:cadmium telluride