title: Histopathologic changes in the uterus, cervix and vagina of immature CD-1 mice exposed to low doses of perfluorooctanoic acid (PFOA) in a uterotrophic assay.
abstract: The estrogenic and antiestrogenic potential of perfluorooctanoic acid (PFOA) was assessed using an immature mouse uterotrophic assay and by histologic evaluation of the uterus, cervix and vagina following treatment. Female offspring of CD-1 dams were weaned at 18days old and assigned to groups of equal weight, and received 0, 0.01, 0.1, or 1mg PFOA/kg BW/d by gavage with or without 17-β estradiol (E(2), 500μg/kg/d) from PND 18-20 (n=8/treatment/block). At 24h after the third dose (PND 21), uteri were removed and weighed. Absolute and relative uterine weights were significantly increased in the 0.01mg/kg PFOA only group. Characteristic estrogenic changes were present in all E(2)-treated mice; however, they were minimally visible in the 0.01 PFOA only mice. These data suggest that at a low dose PFOA produces minimal histopathologic changes in the reproductive tract of immature female mice, and does not antagonize the histopathologic effects of E(2).
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Electronic address: dixon@niehs.nih.gov.
authors.forename: Darlene
authors.initials: D
authors.lastname: Dixon
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Casey E
authors.initials: CE
authors.lastname: Reed
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Alicia B
authors.initials: AB
authors.lastname: Moore
authors.affiliation: Toxicology Curriculum, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Gibbs-Flournoy
authors.affiliation: Environmental Media Assessment Group, National Center for Environmental Assessment (NCEA), Office of Research and Development (ORD), U.S. EPA, Research Triangle Park, NC 27711, USA.
authors.forename: Erin P
authors.initials: EP
authors.lastname: Hines
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Wallace
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Jason P
authors.initials: JP
authors.lastname: Stanko
authors.affiliation: Social & Scientific Systems, Inc., Durham, NC 27703, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Laboratory of Reproductive and Developmental Toxicology, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Wendy N
authors.initials: WN
authors.lastname: Jefferson
authors.affiliation: NTP (retired), National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.
authors.forename: Retha R
authors.initials: RR
authors.lastname: Newbold
authors.affiliation: National Toxicology Program (NTP) Laboratories Branch, Division of the NTP, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA. Electronic address: fentonse@niehs.nih.gov.
authors.forename: Suzanne E
authors.initials: SE
authors.lastname: Fenton
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001681:Biological Assay; D002210:Caprylates / Q000633:toxicity*; D002584:Cervix Uteri / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D014599:Uterus / Q000187:drug effects* / Q000473:pathology*; D014621:Vagina / Q000187:drug effects* / Q000473:pathology*
chemical_list: D002210:Caprylates; D052244:Endocrine Disruptors; D005466:Fluorocarbons; D004958:Estradiol; C023036:perfluorooctanoic acid