title: Positional identity of murine mesenchymal stem cells resident in different organs is determined in the postsegmentation mesoderm.
abstract: Although mesenchymal stem cells (MSCs) of distinct tissue origin have a large number of similarities and differences, it has not been determined so far whether tissue-resident MSCs are the progenies of one ancestor cell lineage or the results of parallel cell developmental events. Here we compared the expression levels of 177 genes in murine MSCs derived from adult and juvenile bone marrow and adult adipose tissue, as well as juvenile spleen, thymus, and aorta wall by quantitative real-time polymerase chain reaction and the results were partially validated at protein level. All MSC lines uniformly expressed a large set of genes including well-known mesenchymal markers, such as α-smooth muscle actin, collagen type I α-chain, GATA6, Mohawk, and vimentin. In contrast, pluripotency genes and the early mesodermal marker T-gene were not expressed. On the other hand, different MSC lines consistently expressed distinct patterns of Hox genes determining the positional identity of a given cell population. Moreover, MSCs of different origin expressed a few other transcription factors also reflecting their topological identity and so the body segment or organ to which they normally contributed in vivo: (1) thymus-derived cells specifically expressed Tbx5 and Pitx2; (2) spleen-derived MSCs were characterized with Tlx1 and Nkx2.5; (3) Pitx1 designated femoral bone marrow cells and (4) En2 appeared in aorta wall-derived MSCs. Thus, MSCs exhibited topographic identity and memory even after long-term cultivation in vitro. On the basis of these results, we suggest that postnatal MSCs isolated from different anatomical sites descend from precursor cells developing in the postsegmentation mesoderm.
authors.affiliation: National Blood Service, Stem Cell Biology Unit, Budapest, Hungary.
authors.forename: Bernadett
authors.initials: B
authors.lastname: Sági
authors.forename: Pouneh
authors.initials: P
authors.lastname: Maraghechi
authors.forename: Veronika S
authors.initials: VS
authors.lastname: Urbán
authors.forename: Beáta
authors.initials: B
authors.lastname: Hegyi
authors.forename: Anna
authors.initials: A
authors.lastname: Szigeti
authors.forename: Roberta
authors.initials: R
authors.lastname: Fajka-Boja
authors.forename: Gyöngyi
authors.initials: G
authors.lastname: Kudlik
authors.forename: Katalin
authors.initials: K
authors.lastname: Német
authors.forename: Eva
authors.initials: E
authors.lastname: Monostori
authors.forename: Elen
authors.initials: E
authors.lastname: Gócza
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000254:growth & development; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology; D019070:Cell Lineage / Q000235:genetics; D002478:Cells, Cultured; D016000:Cluster Analysis; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008648:Mesoderm / Q000166:cytology* / Q000254:growth & development; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000254:growth & development; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development; D013997:Time Factors; D000099266:Engrailed 2 Protein
chemical_list: D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051761:Paired Box Transcription Factors; D020825:T-Box Domain Proteins; D000099266:Engrailed 2 Protein; C103172:T-box transcription factor 5; C487378:Tlx1 protein, mouse; C101485:homeobox protein PITX1