title: Crystallization and preliminary X-ray crystallographic analysis of β-ketoacyl-ACP synthase I (XoFabB) from Xanthomonas oryzae pv. oryzae.
abstract: The proteins in the fatty-acid synthesis pathway in bacteria have significant potential as targets for the development of antibacterial agents. An essential elongation step in fatty-acid synthesis is performed by β-ketoacyl-acyl carrier protein synthase I (FabB). The organism Xanthomonas oryzae pv. oryzae (Xoo) causes a destructive bacterial blight disease of rice. The XoFabB protein from Xoo was expressed, purified and crystallized for the three-dimensional structure determination that is essential for the development of specific inhibitors of the enzyme. An XoFabB crystal diffracted to 3.0 Å resolution and belonged to the tetragonal space group P4(1), with unit-cell parameters a = b = 82.2, c = 233.2 Å. Assuming that the crystallographic structure contains four molecules in the asymmetric unit, the corresponding V(M) would be 2.18 Å(3) Da(-1) and the solvent content would be 43.5%. The initial structure was determined by the MOLREP program with an R factor of 44.0% and does contain four monomers in the asymmetric unit.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
authors.forename: Thanh Thi Ngoc
authors.initials: TT
authors.lastname: Doan
authors.forename: Jin Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Qui Khanh
authors.initials: QK
authors.lastname: Mac
authors.forename: Cheolwon
authors.initials: C
authors.lastname: Chung
authors.forename: Natarajan
authors.initials: N
authors.lastname: Sampath
authors.forename: Jeong Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Yeh Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Lin Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry*; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; C078182:beta-ketoacyl-acyl carrier protein synthase I; D015099:3-Oxoacyl-(Acyl-Carrier-Protein) Synthase