title: Anaerosalibacter bizertensis gen. nov., sp. nov., a halotolerant bacterium isolated from sludge.
abstract: A strictly anaerobic, halotolerant and thermotolerant strain, designated C5BEL(T), was isolated in north Tunisia from storage tanks holding waste generated by the recycling of discarded motor oils. Cells of strain C5BEL(T) were Gram-stain-positive, motile by laterally inserted flagella, straight, and spore-forming. Their two major fatty acids were iso-C(15 : 0) and iso-C(15 : 0) dimethyl acetal. Growth was observed at temperatures of 25-55 °C (optimum, 40 °C) and at pH 6-9 (optimum, pH 7.5). The salinity range for growth was 0-100 g l(-1) NaCl (optimum, 5 g l(-1)). Yeast extract was required for growth. Strain C5BEL(T) was heterotrophic, able to use glucose, pyruvate, succinate, yeast extract, bio-trypticase and peptone, but unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content of strain C5BEL(T) was 31.1 mol%. 16S rRNA gene sequence analysis indicated that strain C5BEL(T) was a member of the family Clostridiaceae, class Clostridia, phylum Firmicutes and was most closely related to Sporanaerobacter acetigenes Lup33(T) ( = DSM 13106(T)) (92.4 % similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain C5BEL(T) can be classified as a novel species in a new genus, for which the name Anaerosalibacter bizertensis gen. nov., sp. nov. is proposed. The type strain of the type species is C5BEL(T) ( = DSM 23801(T) = JCM 17239(T)).
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.|Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis (IPT), BP 74, 13 place Pasteur, Belvédère-1002, Tunis, Tunisia.
authors.forename: Raja
authors.initials: R
authors.lastname: Rezgui
authors.affiliation: Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis (IPT), BP 74, 13 place Pasteur, Belvédère-1002, Tunis, Tunisia.
authors.forename: Abderrazak
authors.initials: A
authors.lastname: Maaroufi
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
authors.forename: Zouhaier
authors.initials: Z
authors.lastname: Ben Ali Gam
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Laboratoire de Microbiologie, Groupe des Bioprocédés, Institut Pasteur de Tunis (IPT), BP 74, 13 place Pasteur, Belvédère-1002, Tunis, Tunisia.
authors.forename: Saïd
authors.initials: S
authors.lastname: Ben Hamed
authors.affiliation: Laboratoire de Microbiologie IRD, Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MI0, UM110, 163 avenue de Luminy, case 925, F-13288 Marseille cedex 9, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Labat
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010578:Petroleum / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014416:Tunisia
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012722:Sewage