title: Suppression of microRNA-96 expression inhibits the invasion of hepatocellular             carcinoma cells.
abstract: MicroRNA-96 (miR-96) expression is dysregulated in certain types of cancers.             However, the role of miR-96 in hepatocellular carcinoma (HCC) invasion and metastasis             remains elusive. miR-96 expression was investigated in a number of HCC cell lines             by quantitative reverse transcription‑polymerase chain reaction (RT-PCR). Cell             invasive ability of metastatic HCCLM6 cells transfected with anti-miR-96 oligonucleotides             or miR-96 mimic was determined by Matrigel invasion assay in vitro. In addition,             metastasis-associated protein, osteopontin, was evaluated by Western blotting.             miR-96 expression was significantly increased in highly metastatic HCCLM6 cells.             Decreasing miR-96 expression with anti-miR-96 oligonucleotides led to reduced             migration and invasion of HCCLM6 cells in vitro. In particular, down-regulation             of miR-96 decreased osteopontin expression in HCCLM6 cells. Suppression of miR-96             expression inhibits the invasion of HCC cells, suggesting that miR-96 may be a             therapeutic target for inhibiting HCC invasion and metastasis.
authors.affiliation: Key Laboratory of Carcinogenesis and Cancer Invasion, Liver Cancer Institute and Zhongshan Hospital, Fudan University, Ministry of Education, Shanghai 200032, PR China.
authors.forename: Rong-Xin
authors.initials: RX
authors.lastname: Chen
authors.forename: Yun-Hong
authors.initials: YH
authors.lastname: Xia
authors.forename: Tong-Chun
authors.initials: TC
authors.lastname: Xue
authors.forename: Sheng-Long
authors.initials: SL
authors.lastname: Ye
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000473:pathology; D020319:Oligodeoxyribonucleotides, Antisense / Q000378:metabolism
chemical_list: C541492:MIRN96 microRNA, human; D035683:MicroRNAs; D020319:Oligodeoxyribonucleotides, Antisense