title: Bacterial cellulose production from cotton-based waste textiles: enzymatic saccharification enhanced by ionic liquid pretreatment.
abstract: Cotton-based waste textiles were explored as alternative feedstock for production of bacterial cellulose (BC) by Gluconacetobacter xylinus. The cellulosic fabrics were treated with the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). [AMIM]Cl caused 25% inactivation of cellulase activity at a concentration as low as of 0.02 g/mL and decreased BC production during fermentation when present in concentrations higher than 0.0005 g/mL. Therefore, removal of residual IL by washing with hot water was highly beneficial to enzymatic saccharification as well as BC production. IL-treated fabrics exhibited a 5-7-fold higher enzymatic hydrolysis rate and gave a seven times larger yield of fermentable sugars than untreated fabrics. BC from cotton cloth hydrolysate was obtained at an yield of 10.8 g/L which was 83% higher than that from the culture grown on glucose-based medium. The BC from G. xylinus grown on IL-treated fabric hydrolysate had a 79% higher tensile strength than BC from glucose-based culture medium which suggests that waste cotton pretreated with [AMIM]Cl has potential to serve as a high-quality carbon source for BC production.
authors.affiliation: Group of Microbiological Engineering and Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China. fhong@dhu.edu.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Hong
authors.forename: Xiang
authors.initials: X
authors.lastname: Guo
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.forename: Shi-fen
authors.initials: SF
authors.lastname: Han
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Leif J
authors.initials: LJ
authors.lastname: Jönsson
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis*; D032561:Cotton Fiber / Q000379:methods*; D020576:Gluconobacter / Q000378:metabolism*; D005947:Glucose / Q000096:biosynthesis*; D007220:Industrial Waste / Q000517:prevention & control*; D052578:Ionic Liquids / Q000737:chemistry*
chemical_list: D007220:Industrial Waste; D052578:Ionic Liquids; D002482:Cellulose; D002480:Cellulase; D005947:Glucose