title: A new bacterial co-expression system for over-expressing soluble protein and validating protein-protein interaction.
abstract: Toxic, membrane, and hydrophobic proteins are usually difficult to individually over-express in Escherichia coli because they require a binding-partner protein for folding and stability. To obtain these types of soluble proteins or protein complexes, protein co-expression is used. Such co-expression systems are extremely suitable for the high-throughput validation of protein-protein interactions. In a previous study, we developed a novel co-expression vector, pHEX, which is compatible, and thus can be partnered, with many commercially available E. coli vectors, such as pGEX and pMAL. Either of the vectors allows proteins to be expressed individually as a tagged fusion protein and can be used directly for protein co-purification. This protocol presents the experimental procedure for the co-expression method.
authors.affiliation: Huazhong Agricultural University, Wuhan, China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zeng
authors.forename: Zheng-Guo
authors.initials: ZG
authors.lastname: He
mesh_terms: D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D009841:Oligonucleotides / Q000235:genetics; D017510:Protein Folding; D055550:Protein Stability; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012995:Solubility; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D046912:Multiprotein Complexes; D009841:Oligonucleotides; D011994:Recombinant Proteins