title: Isolation of bluetongue virus serotype 1 from Culicoides vector captured in livestock farms and sequence analysis of the viral genome segment-2.
abstract: Bluetongue virus serotype-1 (BTV-1) was isolated from Culicoides oxystoma vectors captured on livestock farms in two places of Gujarat, India. The viruses were isolated on BHK-21 cells, which produced characteristic BTV-related cytopathic effects between 24 and 48 h post-infection. Virus antigen was demonstrated in infected cells at different passage by a BTV-specific sandwich ELISA. Further, polyacrylamide gel electrophoresis and silver staining of viral genomic RNA revealed ten double-stranded RNA segments characteristic of BTV. Serotype of the isolates was identified by virus neutralization and PCR coupled with sequencing. The isolates were designated as SKN-7 and SKN-8 and their genome segment-2 (VP2) were sequenced. Phylogenetic analyses revealed very close relationship between them although they are not identical. SKN-8 showed closer relationship with a recently isolated BTV-1 from goat. Bluetongue virus was earlier isolated from Culicoides in adjacent state more than 20 years ago, although the serotype of the virus was not determined.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
authors.forename: A I
authors.initials: AI
authors.lastname: Dadawala
authors.forename: S K
authors.initials: SK
authors.lastname: Biswas
authors.forename: W
authors.initials: W
authors.lastname: Rehman
authors.forename: K
authors.initials: K
authors.lastname: Chand
authors.forename: A
authors.initials: A
authors.lastname: De
authors.forename: B S
authors.initials: BS
authors.lastname: Mathapati
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.forename: H C
authors.initials: HC
authors.lastname: Chauhan
authors.forename: B S
authors.initials: BS
authors.lastname: Chandel
authors.forename: B
authors.initials: B
authors.lastname: Mondal
mesh_terms: D000383:Agriculture; D000818:Animals; D001820:Bluetongue virus / Q000145:classification* / Q000302:isolation & purification*; D002460:Cell Line; D002519:Ceratopogonidae / Q000821:virology*; D006224:Cricetinae; D016679:Genome, Viral; D007194:India; D058751:Livestock; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012703:Serotyping
chemical_list: D012367:RNA, Viral