title: Molecular mechanism of active zone organization at vertebrate neuromuscular junctions.
abstract: Organization of presynaptic active zones is essential for development, plasticity, and pathology of the nervous system. Recent studies indicate a trans-synaptic molecular mechanism that organizes the active zones by connecting the pre- and the postsynaptic specialization. The presynaptic component of this trans-synaptic mechanism is comprised of cytosolic active zone proteins bound to the cytosolic domains of voltage-dependent calcium channels (P/Q-, N-, and L-type) on the presynaptic membrane. The postsynaptic component of this mechanism is the synapse organizer (laminin β2) that is expressed by the postsynaptic cell and accumulates specifically on top of the postsynaptic specialization. The pre- and the postsynaptic components interact directly between the extracellular domains of calcium channels and laminin β2 to anchor the presynaptic protein complex in front of the postsynaptic specialization. Hence, the presynaptic calcium channel functions as a scaffolding protein for active zone organization and as an ion-conducting channel for synaptic transmission. In contrast to the requirement of calcium influx for synaptic transmission, the formation of the active zone does not require the calcium influx through the calcium channels. Importantly, the active zones of adult synapses are not stable structures and require maintenance for their integrity. Furthermore, aging or diseases of the central and peripheral nervous system impair the active zones. This review will focus on the molecular mechanisms that organize the presynaptic active zones and summarize recent findings at the neuromuscular junctions and other synapses.
authors.affiliation: Department of Anatomy and Cell Biology, and Kansas Intellectual and Developmental Disabilities Research Center, University of Kansas Medical School, 3901 Rainbow Blvd., MS 3051, HLSIC Rm. 2073, Kansas City, KS 66160, USA. hnishimune@kumc.edu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishimune
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D009469:Neuromuscular Junction / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D057907:Post-Synaptic Density / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D017729:Presynaptic Terminals / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D013570:Synaptic Membranes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*; D014714:Vertebrates / Q000378:metabolism / Q000502:physiology*
chemical_list: D015220:Calcium Channels