title: The use of a flagellar export signal for the secretion of recombinant proteins in Salmonella.
abstract: The flagellum-specific export system is a specialized type III export machinery, which exports external flagellar proteins through the central channel of the flagellar filament. A number of evidence indicates that short segments within the disordered N-terminal region of flagellar axial proteins are recognized by the flagellum-specific export apparatus. Recently, we have demonstrated that the 26-47 segment of Salmonella typhimurium flagellin is capable of mediating flagellar export. N-terminal flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9-40 kDa) facilitating their secreted expression and easy purification from the medium. Certain over-expressed proteins that are easily degraded within the cells are found intact in the medium implying a potential application of this expression system for proteins of high proteolytic susceptibility.
authors.affiliation: Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Veszprém, Hungary. von007@almos.vein.hu
authors.forename: Ferenc
authors.initials: F
authors.lastname: Vonderviszt
authors.forename: Ráchel
authors.initials: R
authors.lastname: Sajó
authors.forename: József
authors.initials: J
authors.lastname: Dobó
authors.forename: Péter
authors.initials: P
authors.lastname: Závodszky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D018274:Electroporation; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D005408:Flagellin