title: Neurochemical properties of aquaporin 1-expressing sensory neurons from the ovine trigeminal ganglion.
abstract: Aims of the present study were to investigate the distribution and morphology of aquaporin 1-immunoreactive (AQP1-IR) neurons in the sensory ganglia of the sheep. Double immunohistochemical staining was applied to figure out whether substance P (SP), calcitonin gene-related peptide (CGRP) and galanin are present in AQP1-bearing primary afferent neurons. The expression of AQP1 was present only in trigeminal ganglion, whereas in nodose ganglion, jugular ganglion as well as C(1) -C(7) dorsal root ganglia no presence of AQP1 was found. In trigeminal ganglion, 15.4 ± 2.3% of Hu C/D-IR neurons (pan-neuronal marker) showed the presence of AQP1. The vast majority of AQP1-IR trigeminal sensory neurons (approximately 69.6 ± 3.3%, n = 5) were classified as middle in size, 28.6 ± 3.0% of AQP1-IR neurons were small and only 1.8 ± 0.6% of AQP1-positive neurons were large in size. Amongst the population of AQP1-IR trigeminal neurons as many as 58.5 ± 3.9% were immunopositive to SP, 30.7 ± 2.3% showed the presence of CGRP and 10.9 ± 0.2% coexpressed galanin. In trigeminal ganglion, SP-IR as well as CGRP-IR (but not galanin-IR) nerve fibres were found in close neighbourhood of AQP1-IR neurons. It is concluded that AQP1 is present in certain neuronal subsets of the ovine trigeminal ganglion; however, the exact role of this water channel has to be elucidated.
authors.affiliation: Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland. mb.arciszewski@wp.pl
authors.forename: M B
authors.initials: MB
authors.lastname: Arciszewski
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005260:Female; D019004:Galanin / Q000032:analysis; D005727:Ganglia, Spinal / Q000378:metabolism; D008297:Male; D009620:Nodose Ganglion / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*; D034561:Sheep, Domestic / Q000378:metabolism*; D013373:Substance P / Q000032:analysis; D012668:Trigeminal Ganglion / Q000378:metabolism*
chemical_list: D051398:Aquaporin 1; D013373:Substance P; D019004:Galanin; D015740:Calcitonin Gene-Related Peptide