title: Surface plasmon resonance based biosensors for exploring the influence of alkaloids on aggregation of amyloid-β peptide.
abstract: The main objective of the presented study was the development of a simple analytical tool for exploring the influence of naturally occurring compounds on the aggregation of amyloid-β peptide (Aβ(40)) in order to find potential anti-neurodegenerative drugs. The gold discs used for surface plasmon resonance (SPR) measurements were modified with thioaliphatic acid. The surface functionalized with carboxylic groups was used for covalent attaching of Aβ(40) probe by creation of amide bonds in the presence of EDC/NHS. The modified SPR gold discs were used for exploring the Aβ(40) aggregation process in the presence of selected alkaloids: arecoline hydrobromide, pseudopelletierine hydrochloride, trigonelline hydrochloride and α-lobeline hydrochloride. The obtained results were discussed with other parameters which govern the phenomenon studied such as lipophilicity/hydrophilicy and Aβ(40)-alkaloid association constants.
authors.affiliation: Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland. b.krazinski@pan.olsztyn.pl
authors.forename: Bartłomiej Emil
authors.initials: BE
authors.lastname: Kraziński
authors.forename: Jerzy
authors.initials: J
authors.lastname: Radecki
authors.forename: Hanna
authors.initials: H
authors.lastname: Radecka
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology; D000544:Alzheimer Disease / Q000175:diagnosis; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry* / Q000187:drug effects; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D020349:Surface Plasmon Resonance
chemical_list: D000470:Alkaloids; D016229:Amyloid beta-Peptides; D006046:Gold