title: A polyclonal antibody against active C-terminal ADAMTS-18 fragment.
abstract: The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.
authors.affiliation: Department of Medical Genetics, School of Medicine, Shanghai Jiao Tong University, PR China.
authors.forename: Suying
authors.initials: S
authors.lastname: Dang
authors.forename: Dawei
authors.initials: D
authors.lastname: Bu
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D051722:ADAM Proteins / Q000276:immunology* / Q000494:pharmacology; D000071096:ADAMTS Proteins; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001760:Bleeding Time / Q000379:methods; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D013927:Thrombosis / Q000188:drug therapy / Q000276:immunology; D014611:Vaccination
chemical_list: D000906:Antibodies; D010446:Peptide Fragments; D011994:Recombinant Proteins; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C510099:ADAMTS18 protein, human