title: A cell culture model of facial palsy resulting from reactivation of latent herpes simplex type 1.
abstract: OBJECTIVE
  Reactivation of herpes simplex virus type 1 (HSV-1) in geniculate ganglion neurons (GGNs) is an etiologic mechanism of Bell's palsy (BP) and delayed facial palsy (DFP) after otologic surgery.
  
  
  BACKGROUND
  Several clinical studies, including temporal bone studies, antibody, titers, and intraoperative studies, suggest that reactivation of HSV-1 from latently infected GGNs may lead to both BP and DFP. However, it is difficult to study these processes in humans or live animals.
  
  
  METHODS
  Primary cultures of GGNs were latently infected with Patton strain HSV-1 expressing a green fluorescent protein-late lytic gene chimera. Four days later, these cultures were treated with trichostatin A (TSA), a known chemical reactivator of HSV-1 in other neurons. Cultures were monitored daily by fluorescent microscopy. Titers of media from lytic, latent, and latent/TSA treated GGN cultures were obtained using plaque assays on Vero cells. RNA was harvested from latently infected GGN cultures and examined for the presence of viral transcripts using reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Latently infected GGN cultures displayed latency-associated transcripts only, whereas lytically infected and reactivated latent cultures produced other viral transcripts, as well. The GGN cultures displayed a reactivation rate of 65% after treatment with TSA. Media from latently infected cultures contained no detectable infectious HSV-1, whereas infectious virus was observed in both lytically and latently infected/TSA-treated culture media.
  
  
  CONCLUSIONS
  We have shown that cultured GGNs can be latently infected with HSV-1, and HSV-1 in these latently infected neurons can be reactivated using TSA, yielding infectious virus. These results have implications for the cause of both BP and DFP.
authors.affiliation: Department of Otolaryngology, New York University School of Medicine, New York, New York 10016, USA.
authors.forename: Maggie A
authors.initials: MA
authors.lastname: Kuhn
authors.forename: Shruti
authors.initials: S
authors.lastname: Nayak
authors.forename: Vladimir
authors.initials: V
authors.lastname: Camarena
authors.forename: Jimmy
authors.initials: J
authors.lastname: Gardner
authors.forename: Angus
authors.initials: A
authors.lastname: Wilson
authors.forename: Ian
authors.initials: I
authors.lastname: Mohr
authors.forename: Moses V
authors.initials: MV
authors.lastname: Chao
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Roehm
mesh_terms: D000818:Animals; D020330:Bell Palsy / Q000209:etiology / Q000517:prevention & control / Q000821:virology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003470:Culture Media; D005158:Facial Paralysis / Q000209:etiology* / Q000517:prevention & control / Q000821:virology*; D005830:Geniculate Ganglion / Q000166:cytology / Q000821:virology; D049452:Green Fluorescent Proteins; D018259:Herpesvirus 1, Human; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000821:virology; D012367:RNA, Viral / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic; D014709:Vero Cells; D014775:Virus Activation; D017735:Virus Latency / Q000235:genetics
chemical_list: D003470:Culture Media; D012367:RNA, Viral; D049452:Green Fluorescent Proteins