title: Anti-inflammatory effect of heme oxygenase-1 toward Porphyromonas gingivalis lipopolysaccharide in macrophages exposed to gomisins A, G, and J.
abstract: Periodontitis, a chronic inflammatory periodontal disease that develops from gingivitis, is caused by periodontal pathogenic bacteria such as Porphyromonas gingivalis. Recent studies have focused on the antioxidant, anti-human immunodeficiency virus, anticarcinogenic, and anti-inflammatory properties of gomisins. However, the anti-inflammatory activities of gomisin plants through heme oxygenase-1 (HO-1) signals remain poorly defined. We found that gomisins' anti-inflammatory activity occurs via the induction of HO-1 expression. Gomisins G and J inhibit the production of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 and also block nuclear factor-κB activation in Raw264.7 cells stimulated with P. gingivalis lipopolysaccharide. Furthermore, pro-inflammatory cytokine production is inhibited through the induction of HO-1 expression. HO-1 expression is induced by all gomisins, but their anti-inflammatory activity via HO-1 signaling is observed with gomisins G and J, and not A. We found that gomisins G and J extracted from Schisandria chinensis can inhibit the P. gingivalis lipopolysaccharide induced-inflammatory responses in Raw264.7 cells.
authors.affiliation: Bio-IT Fusion Technology Research Institute, Pusan National University, Busan, Korea.
authors.forename: Eun Yeon
authors.initials: EY
authors.lastname: Ryu
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Park
authors.forename: Sun Gun
authors.initials: SG
authors.lastname: Kim
authors.forename: Da Jung
authors.initials: DJ
authors.lastname: Park
authors.forename: Jum Soon
authors.initials: JS
authors.lastname: Kang
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Kim
authors.forename: Rajaseker
authors.initials: R
authors.lastname: Seetharaman
authors.forename: Young-Whan
authors.initials: YW
authors.lastname: Choi
authors.forename: Sang-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D034242:Cyclooctanes / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D017705:Lignans / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000472:pathogenicity; D032141:Schisandra / Q000737:chemistry; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D004149:Dioxoles; D051547:Heme Oxygenase-1; D053583:Interleukin-1beta; D015850:Interleukin-6; D017705:Lignans; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D016328:NF-kappa B; D010936:Plant Extracts; D011083:Polycyclic Compounds; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C033585:schizandrol B; C034557:schizandrer A; C496542:Hmox1 protein, mouse; C082091:gomisin J