title: Expression, purification and preliminary crystallographic analysis of Rv2247, the β subunit of acyl-CoA carboxylase (ACCD6) from Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis (Mtb) acyl-CoA carboxylase is involved in the biosynthesis of mycolic acids, which are a key component of the bacillus cell wall. The Mtb genome encodes six acyl-CoA carboxylase β subunits (ACCD1-6), three of which (ACCD4-6) are essential for survival of the pathogen on minimal medium. Mtb ACCD6 has been expressed, purified and crystallized. The two forms of Mtb ACCD6 crystals belonged to space groups P4(1)2(1)2 and P2(1)2(1)2(1) and diffracted to 2.9 and 2.5 Å resolution, respectively, at a synchrotron-radiation source.
authors.affiliation: Department of Biochemistry, School of Molecular and Systems Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Chunying
authors.initials: C
authors.lastname: Niu
authors.forename: Jiang
authors.initials: J
authors.lastname: Yin
authors.forename: Maia M
authors.initials: MM
authors.lastname: Cherney
authors.forename: Michael N G
authors.initials: MN
authors.lastname: James
mesh_terms: D000595:Amino Acid Sequence; D019735:Carbon-Carbon Ligases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D015870:Gene Expression; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D021122:Protein Subunits; D019735:Carbon-Carbon Ligases; C018757:acyl-CoA carboxylase