title: Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein.
abstract: Tomato mosaic virus belongs to the genus Tobamovirus in the alphavirus-like superfamily of positive-strand RNA viruses. The alphavirus-like superfamily includes many plant and animal viruses of agronomical and clinical importance. These viruses encode replication-associated proteins that contain a putative superfamily 1 helicase domain. No three-dimensional structures for this domain have been determined to date. Here, the crystallization and preliminary X-ray diffraction analysis of the 130K helicase domain are reported. Diffraction data were collected and processed to 2.05 and 1.75 Å resolution from native and selenomethionine-labelled crystals, respectively. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 85.8, b = 128.3, c = 40.7 Å.
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xiang
authors.forename: Mayumi
authors.initials: M
authors.lastname: Niiyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Etsuko
authors.initials: E
authors.lastname: Katoh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D020365:RNA Helicases / Q000737:chemistry*; D017898:Tobamovirus / Q000201:enzymology*
chemical_list: D020365:RNA Helicases