title: Phage-chips for novel optically readable tissue engineering assays.
abstract: We report novel phage-based array chips that are optically readable for cell proliferation and morphology assays. Using M13 phages that were engineered to display RGD on its major coat proteins and/or immobilize FGFb on its minor coat proteins, we prepared arrays of phage spot matrices composed of self-assembled nanofibrous network structures. We cultured fibroblasts on the arrays and, using surface plasmon resonance (SPR) spectroscopy, monitored the effects of the biochemical cues displayed by the phage on cell proliferation and morphology. This study demonstrates the utility of engineered phages as promising coating materials for lab-on-a-chip (LOC) platforms, allowing sensitive monitoring of the effects of functional peptides on cell growth. Phage-chips have great potential for use as high-throughput screening systems for biochemical assays and biosensors and the discovery of novel drugs.
authors.affiliation: Department of Bioengineering, and Berkeley Nanoscience and Nanoengineering Institute, University of California, Berkeley, Berkeley, California 94720, USA.
authors.forename: So Young
authors.initials: SY
authors.lastname: Yoo
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Oh
authors.forename: Seung-Wuk
authors.initials: SW
authors.lastname: Lee
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics*; D001710:Biotin / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation; D005346:Fibroblast Growth Factors / Q000737:chemistry / Q000494:pharmacology; D005818:Genetic Engineering; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D016023:Integrins / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000378:metabolism; D055620:Optical Phenomena; D019151:Peptide Library; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D023822:Tissue Engineering / Q000295:instrumentation*
chemical_list: D055767:Immobilized Proteins; D016023:Integrins; D009842:Oligopeptides; D019151:Peptide Library; D005346:Fibroblast Growth Factors; D001710:Biotin; C047981:arginyl-glycyl-aspartic acid