title: Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry.
abstract: This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R(2) (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.
authors.affiliation: School of Electronics and Computer Sciences, University of Southampton, Southampton, SO17 1BJ United Kingdom.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Han
authors.forename: Cees
authors.initials: C
authors.lastname: van Berkel
authors.forename: James
authors.initials: J
authors.lastname: Gwyer
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Capretto
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
mesh_terms: D001772:Blood Cell Count / Q000295:instrumentation*; D017097:Electric Impedance; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology; D044085:Microfluidics; D009000:Monocytes / Q000166:cytology