title: Intrauterine growth restriction leads to changes in sulfur amino acid metabolism, but not global DNA methylation, in Yucatan miniature piglets.
abstract: Intrauterine growth restriction (IUGR), in both animals and humans, has been linked to metabolic syndrome later in life. There has been recent evidence that perturbations in sulfur amino acid metabolism may be involved in this early programming phenomenon. Methionine is the precursor for cellular methylation reactions and for the synthesis of cysteine. It has been suggested that the mechanism behind the "fetal origins" of adult diseases may be epigenetic, involving DNA methylation. Because we have recently demonstrated the fetal origins phenomenon in Yucatan miniature swine, we hypothesized that sulfur amino acid metabolism is altered in IUGR piglets. In this study, metabolites and the activities of sulfur amino acid cycle enzymes were analyzed in liver samples of 3- to 5-day-old runt (IUGR: 0.85±0.13 kg) and large (1.36±0.21 kg) Yucatan miniature pig littermates (n=6 pairs). The IUGR piglets had significantly lower specific and total activities of betaine-homocysteine methyltransferase (BHMT) and cystathionine γ-lyase (CGL) than larger littermates (P<.05). Expression of CGL (but not BHMT) mRNA was also lower in IUGR piglets (P<.05). This low CGL reduced cysteine and taurine concentrations in IUGR pigs and led to an accumulation of hepatic cystathionine, with lower homocysteine concentrations. Methylation index and liver global DNA methylation were unaltered. Reduced prenatal growth in Yucatan miniature piglets impairs their remethylation capacity as well as their ability to remove cystathionine and synthesize cysteine and taurine, which could have important implications on long-term health outcomes of IUGR neonates.
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada.
authors.forename: Dylan S
authors.initials: DS
authors.lastname: MacKay
authors.forename: Julie D
authors.initials: JD
authors.lastname: Brophy
authors.forename: Laura E
authors.initials: LE
authors.lastname: McBreairty
authors.forename: Ross A
authors.initials: RA
authors.lastname: McGowan
authors.forename: Robert F
authors.initials: RF
authors.lastname: Bertolo
mesh_terms: D000603:Amino Acids, Sulfur / Q000378:metabolism*; D000818:Animals; D017849:Animals, Inbred Strains; D000831:Animals, Newborn; D050940:Betaine-Homocysteine S-Methyltransferase / Q000235:genetics / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004195:Disease Models, Animal; D044127:Epigenesis, Genetic; D005317:Fetal Growth Retardation / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008745:Methylation; D009929:Organ Size; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D013654:Taurine / Q000378:metabolism
chemical_list: D000603:Amino Acids, Sulfur; D012333:RNA, Messenger; D013654:Taurine; D050940:Betaine-Homocysteine S-Methyltransferase; D003542:Cystathionine gamma-Lyase