title: A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase using guaiacol as substrate.
abstract: In pH 4.0 succinic acid-sodium hydroxide buffer solution, laccase catalyzed the oxidization of guaiacol substrate to form red particles, which exhibited a strong resonance scattering (RS) peak at 590 nm. Under the chosen conditions, as the laccase increased, the RS intensity (ΔI) increased linearly. The ΔI was proportional to laccase activity in the range of 0.10-1.2 U/mL, with a regression equation of ΔI = 734.0 U(laccase) - 9.7, and a detection limit of 0.05 U/mL. This RS method was applied to the detection of laccase activity in real samples, and the results were agreement with those from spectrophotometry.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.
authors.forename: Caina
authors.initials: C
authors.lastname: Jiang
authors.forename: Shaoming
authors.initials: S
authors.lastname: Ling
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D002384:Catalysis; D006139:Guaiacol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000032:analysis* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D006139:Guaiacol; D042845:Laccase