title: Genomic aberrations in normal appearing mucosa fields distal from oral potentially malignant lesions.
abstract: OBJECTIVE
  Oral fields of visually normal and non-dysplastic mucosa (ODFs) may represent the precursors of oral potentially malignant lesions (OPMLs). Aim of the study was to provide new evidence for the concept of the "field carcinogenesis" model by comparing the ODF and OPML genomic aberration profiles obtained by high resolution DNA flow cytometry (hr DNA-FCM) and array-Comparative Genomic Hybridization (a-CGH). A second aim was to investigate if specific CGH aberrations were associated with DNA aneuploidy.
  
  
  METHODS
  Nineteen patients with single OPMLs were recruited for the study. In parallel with obtaining samples of OPML tissue from 11 leukoplakias without dysplasia (nd-OPMLs) and 8 with dysplasia (d-OPMLs), we also obtained samples from distant ODFs. DNA aneuploid nuclei detected by hr DNA-FCM were physically separated, based on DNA content, from the DNA diploid components with a DNA-FCM-Sorter. These relatively pure subpopulations of epithelial nuclei were then submitted to DNA extraction and a-CGH for a genome-wide analysis of DNA copy number aberrations (CNAs).
  
  
  RESULTS
  The frequencies of DNA aneuploidy (DI ≠ 1) among ODFs and OPMLs were respectively 5.3% and 32%. The DI aneuploid values of ODFs and nd-OPMLs were all near-diploid (DI ≠ 1 and DI ≤ 1.4), while for d-OPMLs were high-aneuploid (DI > 1.4) in 40% of the cases. CNA averages were 1.9 in ODFs and 6.5 in OPMLs. The gain of the chromosomal region 20q13.33-qter was observed in 37% of both ODFs and corresponding OPMLs. Additional common regions included 7p22.2-pter, 11p15.5-pter and 16p13.3-pter where gains were observed. Furthermore, gains of 20q13.31-q13.33 and of 5p13.33-pter and loss of 9p21.3 were detected at high frequency (respectively, at 62.5%, 50% and 50%) only in d-OPMLs. In particular, loss at 9p21.3, gain at 5p13.33-pter and gain of 20q13.31-q13.33 were associated with DNA aneuploidy (p = 0.00004; p = 0.0005; p = 0.01).
  
  
  CONCLUSIONS
  ODFs and OPMLs showed common CNAs in specific chromosomal regions suggesting that they may represent early events of the natural history of oral carcinogenesis according to the field effect cancerization and may contribute to the ODF-OPML transition. In addition, loss at 9p21.3 and gains at 5p13.33-pter and 20q13.31-q13.33 may contribute to DNA aneuploidization.
authors.affiliation: Department of Diagnostic Oncology, Biophysics and Cytometry, National Institute for Cancer Research, Genoa, GE, Italy. walter.giaretti@istge.it
authors.forename: W
authors.initials: W
authors.lastname: Giaretti
authors.forename: M
authors.initials: M
authors.lastname: Maffei
authors.forename: M
authors.initials: M
authors.lastname: Pentenero
authors.forename: P
authors.initials: P
authors.lastname: Scaruffi
authors.forename: A
authors.initials: A
authors.lastname: Donadini
authors.forename: E
authors.initials: E
authors.lastname: Di Nallo
authors.forename: D
authors.initials: D
authors.lastname: Malacarne
authors.forename: R
authors.initials: R
authors.lastname: Marino
authors.forename: U
authors.initials: U
authors.lastname: Familiari
authors.forename: S
authors.initials: S
authors.lastname: Coco
authors.forename: G P
authors.initials: GP
authors.lastname: Tonini
authors.forename: P
authors.initials: P
authors.lastname: Castagnola
authors.forename: S
authors.initials: S
authors.lastname: Gandolfo
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm