title: Green fluorescent protein-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria.
abstract: On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9.
authors.affiliation: Department of Environmental Microbiology/Genetics, University of Technology, Berlin, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Arends
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Schiwon
authors.forename: Türkan
authors.initials: T
authors.lastname: Sakinc
authors.forename: Johannes
authors.initials: J
authors.lastname: Hübner
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Grohmann
mesh_terms: D003227:Conjugation, Genetic; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D013194:Staining and Labeling
chemical_list: D049452:Green Fluorescent Proteins