title: In vitro inhibitory potential of selected Malaysian plants against key enzymes involved in hyperglycemia and hypertension.
abstract: BACKGROUND
  This study was conducted to determine the inhibitory potential of selected Malaysian plants against key enzymes related to type 2 diabetes and hypertension.
  
  
  METHODS
  The samples investigated were pucuk ubi (Manihot esculenta), pucuk betik (Carica papaya), ulam raja (Cosmos caudatus), pegaga (Centella asiatica) and kacang botol (Psophocarpus tetragonolobus). The inhibitory potential of hexane and dichloromethane extracts against the enzymes were determined by using alpha-amylase, alpha-glucosidase and angiotensin I-converting enzyme (ACE) inhibition assay.
  
  
  RESULTS
  In alpha-amylase inhibition assay, the inhibitory potential was highest in pucuk ubi for both hexane (59.22%) and dichloromethane extract (54.15%). Hexane extract of pucuk ubi (95.01%) and dichloromethane extract of kacang botol (38.94%) showed the highest inhibitory potential against alpha-glucosidase, while in ACE inhibition assay, the inhibitory potential was highest in hexane extract of pegaga (48.45%) and dichloromethane extract of pucuk betik (59.77%).
  
  
  CONCLUSIONS
  This study suggests a nutraceutical potential of some of these plants for hyperglycemia and hypertension prevention associated with type 2 diabetes.
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. sploh@medic.upm.edu.my
authors.forename: S P
authors.initials: SP
authors.lastname: Loh
authors.forename: O
authors.initials: O
authors.lastname: Hadira
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D019659:Asteraceae; D029441:Carica; D028041:Centella; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000201:enzymology; D007887:Fabaceae; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D019684:Magnoliopsida; D008296:Malaysia; D002365:Manihot; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal; D016896:Treatment Outcome; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D000516:alpha-Amylases; D007703:Peptidyl-Dipeptidase A