title: Regulation of plasma membrane Ca(2+)-ATPase activity by acetylated tubulin: influence of the lipid environment.
abstract: We demonstrated previously that acetylated tubulin inhibits plasma membrane Ca(2+)-ATPase (PMCA) activity in plasma membrane vesicles (PMVs) of rat brain through a reversible interaction. Dissociation of the PMCA/tubulin complex leads to restoration of ATPase activity. We now report that, when the enzyme is reconstituted in phosphatidylcholine vesicles containing acidic or neutral lipids, tubulin not only loses its inhibitory effect but is also capable of activating PMCA. This alteration of the PMCA-inhibitory effect of tubulin was dependent on concentrations of both lipids and tubulin. Tubulin (300μg/ml) in combination with acidic lipids at concentrations >10%, increased PMCA activity up to 27-fold. The neutral lipid diacylglycerol (DAG), in combination with 50μg/ml tubulin, increased PMCA activity >12-fold, whereas tubulin alone at high concentration (≥300μg/ml) produced only 80% increase. When DAG was generated in situ by phospholipase C incubation of PMVs pre-treated with exogenous tubulin, the inhibitory effect of tubulin on PMCA activity (ATP hydrolysis, and Ca(2+) transport within vesicles) was reversed. These findings indicate that PMCA is activated independently of surrounding lipid composition at low tubulin concentrations (<50μg/ml), whereas PMCA is activated mainly by reconstitution in acidic lipids at high tubulin concentrations. Regulation of PMCA activity by tubulin is thus dependent on both membrane lipid composition and tubulin concentration.
authors.affiliation: Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Argentina.
authors.forename: N E
authors.initials: NE
authors.lastname: Monesterolo
authors.forename: M R
authors.initials: MR
authors.lastname: Amaiden
authors.forename: A N
authors.initials: AN
authors.lastname: Campetelli
authors.forename: V S
authors.initials: VS
authors.lastname: Santander
authors.forename: C A
authors.initials: CA
authors.lastname: Arce
authors.forename: J
authors.initials: J
authors.lastname: Pié
authors.forename: C H
authors.initials: CH
authors.lastname: Casale
mesh_terms: D000107:Acetylation; D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology*; D017136:Ion Transport / Q000502:physiology; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*; D010738:Type C Phospholipases / Q000737:chemistry
chemical_list: D008563:Membrane Lipids; D009419:Nerve Tissue Proteins; D014404:Tubulin; D010738:Type C Phospholipases; D053499:Plasma Membrane Calcium-Transporting ATPases; D002118:Calcium