title: Simultaneous saccharification and fermentation (SSF) of Jatropha curcas shells: utilization of co-products from the biodiesel production process.
abstract: Jatropha curcas has great potential as an oil crop for use in biodiesel applications, and the outer shell is rich in lignocellulose that may be converted to ethanol, giving rise to the concept of a biorefinery. In this study, two dilute pretreatments of 0.5% H(2)SO(4) and 1.0% NaOH were performed on Jatropha shells with subsequent simultaneous saccharification and fermentation (SSF) of the pretreated water-insoluble solids (WIS) to evaluate the effect of inhibitors in the pretreatment slurry. A cellulase loading of 15 FPU/g WIS, complimented with an excess of cellobiase (19.25 U/g), was used for SSF of either the washed WIS or the original slurry to determine the effect of inhibitors. Ethanol and glucose were monitored during SSF of 20 g of pretreated biomass. The unwashed slurry showed to have a positive effect on SSF efficiency for the NaOH-pretreated biomass. Maximum efficiencies of glucan conversion to ethanol in the WIS were 40.43% and 41.03% for the H(2)SO(4)- and NaOH-pretreated biomasses, respectively.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa,Viçosa, MG, Brazil. evanvisser@gmail.com
authors.forename: Evan Michael
authors.initials: EM
authors.lastname: Visser
authors.forename: Delly
authors.initials: D
authors.lastname: Oliveira Filho
authors.forename: Marcos Rogério
authors.initials: MR
authors.lastname: Tótola
authors.forename: Marcio Arêdes
authors.initials: MA
authors.lastname: Martins
authors.forename: Valéria Monteze
authors.initials: VM
authors.lastname: Guimarães
mesh_terms: D056804:Biofuels; D000431:Ethanol / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D031286:Jatropha / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D056804:Biofuels; D013464:Sulfuric Acids; C036909:lignocellulose; D000431:Ethanol; D012972:Sodium Hydroxide; D008031:Lignin; D001617:beta-Glucosidase; D005947:Glucose; C033158:sulfuric acid