title: Asymmetric production of nitric oxide in mouse primary cortical mixed glial cell cultures treated with lipopolysaccharide.
abstract: Activated glial cells produce many toxic molecules, including cytokines and nitric oxide (NO). There is evidence that excess NO production plays a key role in neuronal cell death. Previous research has demonstrated that cortical glial cells from the left and right cortices of the brain secrete cytokines asymmetrically. However, no evidence to date exists about whether glial cell-produced NO is produced asymmetrically as well. The results of this study show that NO production and inducible NO synthase gene expression are both significantly higher in the right hemisphere-derived mixed glial cell compared with cultures derived from the left.
authors.affiliation: Department of Microbiology and Immunology, Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xin
authors.forename: Yun
authors.initials: Y
authors.lastname: Su
authors.forename: Ge-Fei
authors.initials: GF
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Kang-Sheng
authors.initials: KS
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse