title: Modulating protein activity and cellular function by methionine residue oxidation.
abstract: The sulfur-containing amino acid residue methionine (Met) in a peptide/protein is readily oxidized to methionine sulfoxide [Met(O)] by reactive oxygen species both in vitro and in vivo. Methionine residue oxidation by oxidants is found in an accumulating number of important proteins. Met sulfoxidation activates calcium/calmodulin-dependent protein kinase II and the large conductance calcium-activated potassium channels, delays inactivation of the Shaker potassium channel ShC/B and L-type voltage-dependent calcium channels. Sulfoxidation at critical Met residues inhibits fibrillation of atherosclerosis-related apolipoproteins and multiple neurodegenerative disease-related proteins, such as amyloid beta, α-synuclein, prion, and others. Methionine residue oxidation is also correlated with marked changes in cellular activities. Controlled key methionine residue oxidation may be used as an oxi-genetics tool to dissect specific protein function in situ.
authors.affiliation: Institute of Cell Biology, Beijing Normal University, Beijing 100875, China. zjcui@bnu.edu.cn
authors.forename: Zong Jie
authors.initials: ZJ
authors.lastname: Cui
authors.forename: Zong Qiang
authors.initials: ZQ
authors.lastname: Han
authors.forename: Zhi Ying
authors.initials: ZY
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D015221:Potassium Channels / Q000378:metabolism; D011328:Prions / Q000378:metabolism; D055503:Protein Multimerization; D017382:Reactive Oxygen Species / Q000378:metabolism; D051844:alpha-Synuclein / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D001053:Apolipoproteins; D015221:Potassium Channels; D011328:Prions; D017382:Reactive Oxygen Species; D051844:alpha-Synuclein; D008715:Methionine; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; C013111:methionine sulfoxide