title: Selection and characterization of tenascin C targeting peptide.
abstract: Since tenascin C is a factor expressed highly in the tumor-associated matrix, it would be a desirable first step for targeting the tumor-specific microenvironment. In fact, a high level of tenascin C expression has been reported in most solid tumors, including lung cancer, colon cancer and glioblastoma. Therefore, the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration. We isolated a peptide that bound to tenascin C by phage display peptide library selection, and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue. We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration. We propose that the tenascin C targeting peptide may be useful as a specific anti-cancer diagnostic and therapeutic tool for most human solid tumors.
authors.affiliation: National Research Laboratory for RNA Cell Biology, Brain Korea 21 Graduate Program for RNA Biology, Institute of Nanosensor and Biotechnology, and Department of Molecular Biology, Dankook University, Yongin 448-701, Korea. iris1774@dankook.ac.kr
authors.forename: Mee Young
authors.initials: MY
authors.lastname: Kim
authors.forename: Ok Ran
authors.initials: OR
authors.lastname: Kim
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Choi
authors.forename: Heuiran
authors.initials: H
authors.lastname: Lee
authors.forename: Keerang
authors.initials: K
authors.lastname: Park
authors.forename: Choon-Taek
authors.initials: CT
authors.lastname: Lee
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.forename: Sunjoo
authors.initials: S
authors.lastname: Jeong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D019063:Tenascin / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D019151:Peptide Library; D010455:Peptides; D019063:Tenascin