title: Gene expression profiling of embryonic human neural stem cells and dopaminergic neurons from adult human substantia nigra.
abstract: Neural stem cells (NSC) with self-renewal and multipotent properties serve as an ideal cell source for transplantation to treat neurodegenerative insults such as Parkinson's disease. We used Agilent's and Illumina Whole Human Genome Oligonucleotide Microarray to compare the genomic profiles of human embryonic NSC at a single time point in culture, and a multicellular tissue from postmortem adult substantia nigra (SN) which are rich in dopaminergic (DA) neurons. We identified 13525 up-regulated genes in both cell types of which 3737 (27.6%) genes were up-regulated in the hENSC, 4116 (30.4%) genes were up-regulated in the human substantia nigra dopaminergic cells, and 5672 (41.93%) were significantly up-regulated in both cell population. Careful analysis of the data that emerged using DAVID has permitted us to distinguish several genes and pathways that are involved in dopaminergic (DA) differentiation, and to identify the crucial signaling pathways that direct the process of differentiation. The set of genes expressed more highly at hENSC is enriched in molecules known or predicted to be involved in the M phase of the mitotic cell cycle. On the other hand, the genes enriched in SN cells include a different set of functional categories, namely synaptic transmission, central nervous system development, structural constituents of the myelin sheath, the internode region of axons, myelination, cell projection, cell somata, ion transport, and the voltage-gated ion channel complex. Our results were also compared with data from various databases, and between different types of arrays, Agilent versus Illumina. This approach has allowed us to confirm the consistency of our obtained results for a large number of genes that delineate the phenotypical differences of embryonic NSCs, and SN cells.
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. hany800@yahoo.com
authors.forename: Hany E S
authors.initials: HE
authors.lastname: Marei
authors.forename: Asma
authors.initials: A
authors.lastname: Althani
authors.forename: Nahla
authors.initials: N
authors.lastname: Afifi
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Michetti
authors.forename: Mario
authors.initials: M
authors.lastname: Pescatori
authors.forename: Roberto
authors.initials: R
authors.lastname: Pallini
authors.forename: Patricia
authors.initials: P
authors.lastname: Casalbore
authors.forename: Carlo
authors.initials: C
authors.lastname: Cenciarelli
authors.forename: Philip
authors.initials: P
authors.lastname: Schwartz
authors.forename: Abd-Elmaksoud
authors.initials: AE
authors.lastname: Ahmed
mesh_terms: D000328:Adult; D019070:Cell Lineage; D016000:Cluster Analysis; D015925:Cryopreservation; D057225:Data Mining / Q000379:methods; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000378:metabolism*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human; D006801:Humans; D008938:Mitosis; D008954:Models, Biological; D058953:Neural Stem Cells / Q000166:cytology*; D019636:Neurodegenerative Diseases / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction; D013378:Substantia Nigra / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D004298:Dopamine