title: Immunostimulatory effects of gold nanorod and silica-coated gold nanorod on RAW 264.7 mouse macrophages.
abstract: In this study, we have investigated immunostimulatory effects of gold nanorod (Au) and silica-coated gold nanorod (SiAu) concerned with inflammatory mediators such as calcium (Ca), hydrogen peroxide, nitric oxide (NO), various cytokines, prostaglandin E2 (PGE2), transcription factors, and immune response gene in RAW 264.7 mouse macrophages. Both Au and SiAu significantly increased the release of Ca, hydrogen peroxide, NO, IL-1α, IL-1β, IL-6, IL-10, IP-10, MCP-1, MCP-3, TNF-α, RANTES, G-CSF, GM-CSF, LIF, MIP-2, VEGF, and PGE2 with enhancing expression of STAT1, STAT3, c-Fos, and COX-2 mRNA in RAW 264.7 cells. The results suggest that Au and SiAu have immunostimulatory properties to strengthen immune reactions via calcium-transcription factors pathway in macrophage.
authors.affiliation: College of Oriental Medicine, Kyungwon University, Seongnam 461-701, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Wansu
authors.initials: W
authors.lastname: Park
authors.forename: Dong Kee
authors.initials: DK
authors.lastname: Yi
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D006046:Gold / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D043942:Nanotubes; D009569:Nitric Oxide / Q000494:pharmacology; D016877:Oxidants / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects; D012822:Silicon Dioxide / Q000494:pharmacology*; D014157:Transcription Factors / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D018925:Chemokines; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D016877:Oxidants; D014157:Transcription Factors; D009569:Nitric Oxide; D012313:RNA; D006046:Gold; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D015232:Dinoprostone; D002118:Calcium