title: Quantitative high-resolution genomic analysis of single cancer cells.
abstract: During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics.
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Hannemann
authors.forename: Sönke
authors.initials: S
authors.lastname: Meyer-Staeckling
authors.forename: Dirk
authors.initials: D
authors.lastname: Kemming
authors.forename: Iris
authors.initials: I
authors.lastname: Alpers
authors.forename: Simon A
authors.initials: SA
authors.lastname: Joosse
authors.forename: Heike
authors.initials: H
authors.lastname: Pospisil
authors.forename: Stefan
authors.initials: S
authors.lastname: Kurtz
authors.forename: Jennifer
authors.initials: J
authors.lastname: Görndt
authors.forename: Klaus
authors.initials: K
authors.lastname: Püschel
authors.forename: Sabine
authors.initials: S
authors.lastname: Riethdorf
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
authors.forename: Burkhard
authors.initials: B
authors.lastname: Brandt
mesh_terms: D045744:Cell Line, Tumor; D055028:Comparative Genomic Hybridization; D066246:ErbB Receptors / Q000235:genetics; D018740:Genetic Heterogeneity; D023281:Genomics / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D016133:Polymerase Chain Reaction; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors