title: Integrative genome-wide expression and promoter DNA methylation profiling identifies a potential novel panel of ovarian cancer epigenetic biomarkers.
abstract: To identify epigenetic-based biomarkers for diagnosis of ovarian cancer we performed MeDIP-Chip in A2780 and CaOV3 ovarian cancer cell lines. Validation by Sequenom massARRAY methylation analysis confirmed a panel of six gene promoters (ARMCX1, ICAM4, LOC134466, PEG3, PYCARD & SGNE1) where hypermethylation discriminated 27 serous ovarian cancer clinical samples versus 12 normal ovarian surface epithelial cells (OSE) (ROC of 0.98). Notably, CpG sites across the transcription start site of a potential long-intergenic non-coding RNA (lincRNA) gene (LOC134466), was shown to be hypermethylated in 81% of serous EOC and could differentiate tumours from OSE (p<0.05). We propose that this potential biomarker panel holds great promise as a diagnostic test for high-grade (Type II) serous ovarian cancer.
authors.affiliation: Cancer Research Program, The Garvan Institute of Medical Research, 384 Victoria St., Darlinghurst, New South Wales 2010, Australia.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Gloss
authors.forename: Kate I
authors.initials: KI
authors.lastname: Patterson
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Barton
authors.forename: Maria
authors.initials: M
authors.lastname: Gonzalez
authors.forename: James P
authors.initials: JP
authors.lastname: Scurry
authors.forename: Neville F
authors.initials: NF
authors.lastname: Hacker
authors.forename: Robert L
authors.initials: RL
authors.lastname: Sutherland
authors.forename: Philippa M
authors.initials: PM
authors.lastname: O'Brien
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Clark
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D018899:CpG Islands / Q000235:genetics; D018284:Cystadenocarcinoma, Serous / Q000235:genetics; D019175:DNA Methylation; D057890:Epigenomics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics*; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D015415:Biomarkers