title: [Comparative effects of menthol and icilin on the induced contraction of the smooth muscles of the vas deferens of normal and castrated rats].
abstract: Non-specific TRPM8 agonist menthol was shown to inhibit voltage- and agonist-evoked contractions of the smooth muscle (SM) of rat vas deferens. Here we compared the action of menthol with the action of more specific TRPM8 agonist icilin on depolarization- (60 mM KCl), carbachol-(CCh) and noradrenalin-(Nor)-evoked contractions of the SM strips from the prostatic and epididymal portions of the vas deferens of normal and castrated (60-137 days) rats. Inhibitory action of menthol (100 microM) and icilin (10 microM) on the amplitude of KCl-, CCh- and Nor-induced contractions of normal as well as castrated rats was similar consisting about 50%, despite castration per se strongly potentiated CCh- and Nor-evoked contractions compared to the control animals. In the epididymal portion of the control animals menthol suppressed KC 1- and CCh-evoked contractions by 46 +/- 5% and 32 +/- 3% and icilin by only 14 +/- 4% and 6 +/- 7%, respectively, whilst after castration both compounds became virtually ineffective. Considering that TRPM8 may localize in the sarcolemma and sarcoplasmic reticulum (SR) membrane and that menthol can also block voltage-gated calcium channels (VGCCs), our data indicate that in the prostatic portion TRPM8 modulates contractility by primarily decreasing the SR Ca2+ stores content, whilst in the epididymal one by both decreasing the SR filling and supporting Ca2+ entry. Drop in the circulation androgens as a result of castration changes the menthol- and icilin-mediated modulation of the rat vas deferens SM contractility via the decrease of the expression of L-type VGCCs and increase of the expression of TRPM8.
authors.forename: I A
authors.initials: IA
authors.lastname: Vladymyrova
authors.forename: I B
authors.initials: IB
authors.lastname: Filippov
authors.forename: Ie M
authors.initials: IeM
authors.lastname: Kuliieva
authors.forename: A
authors.initials: A
authors.lastname: Iurkevych
authors.forename: R
authors.initials: R
authors.lastname: Skryma
authors.forename: N
authors.initials: N
authors.lastname: Prevarskaia
authors.forename: Ia M
authors.initials: IaM
authors.lastname: Shuba
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008297:Male; D008610:Menthol / Q000494:pharmacology*; D018721:Muscarinic Agonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009919:Orchiectomy; D011189:Potassium Chloride / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D011744:Pyrimidinones / Q000494:pharmacology*; D051381:Rats; D050053:TRPM Cation Channels / Q000819:agonists*; D014649:Vas Deferens / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D000316:Adrenergic alpha-Agonists; D018721:Muscarinic Agonists; D011744:Pyrimidinones; D050053:TRPM Cation Channels; C498947:Trpm8 protein, rat; D008610:Menthol; D011189:Potassium Chloride; C490483:icilin