title: Impact of silicon-based quantum dots on the antioxidative system in white muscle of Carassius auratus gibelio.
abstract: Silicon-based quantum dots were intraperitoneally injected in individuals of Carassius auratus gibelio. Their effects on white muscle were investigated by following their distribution and impact on the antioxidative system. The GSH level significantly increased after 1 and 3 days of exposure by, respectively, 85.3 and 25.4%. Seven days later, GSH levels were similar to control concentrations. MDA concentration rose after three days by 46.9% and remained at the same level after 7 days. Protein thiol levels significantly decreased by 6.7 and 8.1% after 3 and 7 days, whereas advanced oxidation protein products increased by 12.7, respectively, 28.1% in the same time intervals. The protein reactive carbonyl groups were raised only after the first day of exposure and returned to the control level later on. SOD specific activity increased up to 48% after 7 days, while CAT activity increased by 328, 176, and 26% after 1, 3, and 7 days of treatment. GST specific activity was up-regulated by 87, 18, and 9%, while GR activity increased by 68, 34, and 9%. G6PD activity was up-regulated by 12, 22, and 50%, whereas GPx activity raised by 75 and 109% compared to control after, respectively, 1, 3, and 7 days. Our results suggest that oxidative stress induced by silicon-based quantum dots was not strong enough to cause permanent damage in the white muscle of crucian carp.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Loredana
authors.initials: L
authors.lastname: Stanca
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Sorina Nicoleta
authors.initials: SN
authors.lastname: Petrache
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Radu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.|Department of Preclinical Sciences, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 105 Splaiul Independetei, 050097, Bucharest, Romania.
authors.forename: Andreea Iren
authors.initials: AI
authors.lastname: Serban
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Munteanu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Daniela
authors.initials: D
authors.lastname: Teodorescu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Andreea Cristina
authors.initials: AC
authors.lastname: Staicu
authors.affiliation: Laser Department, National Institute of Laser, Plasma and Radiation Physics, 409 Atomistilor, 077125, Bucharest-Magurele, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Sima
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.affiliation: Laser Department, National Institute of Laser, Plasma and Radiation Physics, 409 Atomistilor, 077125, Bucharest-Magurele, Romania.
authors.forename: Constantin
authors.initials: C
authors.lastname: Grigoriu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania.
authors.forename: Otilia
authors.initials: O
authors.lastname: Zarnescu
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independetei, 050095, Bucharest, Romania. dinischiotu@yahoo.com.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D045663:Quantum Dots; D012825:Silicon / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014131:Trace Elements / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D014131:Trace Elements; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D012825:Silicon