title: Identification of cancer stem cell-related microRNAs in hepatocellular carcinoma.
abstract: Cancer Stem cells (CSCs) are the source of many solid tumor types including hepatocellular carcinoma. MicroRNAs (miRNAs) are small noncoding RNAs and have been showed to be associated with hepatic CSCs. Here, we described methods to screen hepatic CSC-related miRNAs, and to validate and examine their expressions and functions in vitro and in vivo, which contribute to the maintenance of stemness and differentiation of hepatic CSCs.
authors.affiliation: Laboratory of Human Carcinogenesis, Bethesda, MD, USA.
authors.forename: Junfang
authors.initials: J
authors.lastname: Ji
authors.forename: Xin Wei
authors.initials: XW
authors.lastname: Wang
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D015197:Carcinogenicity Tests; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000473:pathology; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D035683:MicroRNAs