title: Analysis of the site-specific integration system of the Streptomyces aureofaciens phage μ1/6.
abstract: The bacteriophage μ1/6 integrates its DNA into the chromosome of tetracycline producing strains of Streptomyces                         aureofaciens by a site-specific recombination process. A bioinformatic analysis of the μ1/6 genome revealed that orf5 encodes a putative integrase, a basic protein of 416 amino acids. The μ1/6 integrase was found to belong to the integrase family of site-specific tyrosine recombinases. The phage attachment site (attP) was localized downstream of the int gene. The attachment junctions (attL and attR) were determined, allowing identification of the bacterial attachment site (attB). All attachment sites shared a 46-bp common core sequence within which a site-specific recombination occurs. This core sequence comprises the 3' end of a putative tRNA(Thr) gene (anticodon TGT) which is completely restored in attL after integration of the phage into the host genome. An integration vector containing μ1/6 int-attP region was inserted stably into the S. aureofaciens B96, S. lividans TK24, and S. coelicolor A3. The μ1/6 integrase was shown to be functional in vivo in heterologous Escherichia coli without any other factors encoded by Streptomyces. In vitro recombination assay using purified μ1/6 integrase demonstrated its ability to catalyze integrative recombination in the presence of a crude extract of E. coli cells.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic. jarmila.farkasovska@savba.sk
authors.forename: Jarmila
authors.initials: J
authors.lastname: Farkašovská
authors.forename: Andrej
authors.initials: A
authors.lastname: Godány
mesh_terms: D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000235:genetics / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D042822:Genomic Instability; D019426:Integrases / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013304:Streptomyces aureofaciens / Q000821:virology*; D048372:Streptomyces coelicolor / Q000235:genetics; D048170:Streptomyces lividans / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D016662:Virus Integration
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D014764:Viral Proteins; D019426:Integrases