title: Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation.
abstract: NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial role in myogenesis. Furthermore, inhibition of skNAC expression in myoblasts via specific siRNAs might lead to disturbed incorporation of myosin heavy chain (MyHC) into sarcomeres, however, it has no inhibitory effect on absolute MyHC protein levels. Taken together, our data suggest that skNAC regulates specific aspects of myogenesis.
authors.affiliation: Charité - University Medicine Berlin, Institute of Physiology, Thielallee 71, D-14195 Berlin, Germany.
authors.forename: Felicitas
authors.initials: F
authors.lastname: Berger
authors.forename: Janine
authors.initials: J
authors.lastname: Berkholz
authors.forename: Theresia
authors.initials: T
authors.lastname: Breustedt
authors.forename: Daniela
authors.initials: D
authors.lastname: Ploen
authors.forename: Barbara
authors.initials: B
authors.lastname: Munz
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D051379:Mice; D018832:Molecular Chaperones / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D012038:Regeneration; D012518:Sarcomeres / Q000378:metabolism; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D047428:Protein Kinase Inhibitors; D034741:RNA, Small Interfering; C096099:nascent-polypeptide-associated complex; D003609:Dactinomycin; D003513:Cycloheximide; D048051:p38 Mitogen-Activated Protein Kinases; D018995:Myosin Heavy Chains