title: Rapid detection of dysfunctional high-density lipoproteins using isoelectric focusing-based microfluidic device to diagnose senescence-related disease.
abstract: Recently, we reported elevated levels of advanced glycated end products (AGEs) in human high-density lipoproteins (HDL), with fragmentation of apoA-I in an elderly group, compared with a younger group. More dysfunctional HDL from human plasma was demonstrated in the elderly group, including reconstituted HDL containing glycated apoA-I (gA-I-rHDL) with elevation of AGEs. Based on SDS-PAGE analysis, HDL(3) from the elderly group (E-HDL(3)) exhibited increased multimerization with increased smear band intensity compared to HDL(3) from the younger group (Y-HDL(3)). According to isoelectric focusing gel analysis, gA-I-rHDL and E-HDL(3) showed electromobility to the basic region of pH with a broader band range. In a microfluidic channel, E-HDL(3) had faster mobility with a broader range and a higher isoelectric point (pI, approximately 8.1), whereas Y-HDL(3) showed a narrow band range with a lower pI (approximately 6.9). In conclusion, gA-I-rHDL and E-HDL share several electrophoretic properties with multimerization and faster mobility in microfluidic channels, depending on the isoelectric point. These results can be applied to develop a rapid detection system for modified HDL to predict the extent of aging and aging-related metabolic diseases, such as cardiovascular disease and diabetes.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Wookju
authors.initials: W
authors.lastname: Jang
authors.forename: Jaesool
authors.initials: J
authors.lastname: Shim
authors.forename: Dong-Yeon
authors.initials: DY
authors.lastname: Lee
authors.forename: Prashanta
authors.initials: P
authors.lastname: Dutta
authors.forename: Jae-Ryong
authors.initials: JR
authors.lastname: Kim
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Cho
mesh_terms: D000368:Aged; D000375:Aging / Q000378:metabolism*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D016632:Apolipoprotein A-I / Q000032:analysis / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D002021:Buffers; D003198:Computer Simulation; D004194:Disease; D017127:Glycation End Products, Advanced / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000737:chemistry; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D016632:Apolipoprotein A-I; D015415:Biomarkers; D002021:Buffers; D017127:Glycation End Products, Advanced; D008075:Lipoproteins, HDL