title: [Detecting overexpression level of HER2 gene in NSCLC by real-time quantitative PCR and the 2[-Delta Delta C(T)] method].
abstract: OBJECTIVE
  The evaluation of the expression level of the HER2 gene for the diagnosis and treatment of tumors is usually conducted using immunohistochemical techniques. The aim of the current study is to explore the feasibility of real-time quantitative PCR and the 2[-Delta Delta C(T)] method in detecting the level of HER2 gene overexpression in non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Real-time quantitative PCR and the 2[-Delta Delta C(T)] method were used to detect the level of HER2 gene overexpression in 212 lung cancer and matched non-tumor tissue specimens.
  
  
  RESULTS
  The expression level of HER2 gene in lung cancer tissue was higher than that in the matched non-tumor tissue, with an overexpression rate of 34%.
  
  
  CONCLUSIONS
  Real-time quantitative PCR and the 2[-Delta Delta C(T)] method can be used to detect the level of HER2 gene overexpression in NSCLC.
authors.affiliation: Department of Social Medicine and Health Services Management, School of Public Health, University of South China, Hengyang 421001, China. shuidong_f@hotmail.com
authors.forename: Shuidong
authors.initials: S
authors.lastname: Feng
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Tan
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ling
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Yuan
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human