title: Characterization of a new mouse model for peripheral T cell lymphoma in humans.
abstract: Peripheral T cell lymphomas (PTCLs) are associated with a poor prognosis due to often advanced disease at the time of diagnosis and due to a lack of efficient therapeutic options. Therefore, appropriate animal models of PTCL are vital to improve clinical management of this disease. Here, we describe a monoclonal CD8(+) CD4(-) αβ T cell receptor Vβ2(+) CD28(+) T cell lymphoma line, termed T8-28. T8-28 cells were isolated from an un-manipulated adult BALB/c mouse housed under standard pathogen-free conditions. T8-28 cells induced terminal malignancy upon adoptive transfer into syngeneic BALB/c mice. Despite intracellular expression of the cytotoxic T cell differentiation marker granzyme B, T8-28 cells appeared to be defective with respect to cytotoxic activity as read-out in vitro. Among the protocols tested, only addition of interleukin 2 in vitro could partially compensate for the in vivo micro-milieu in promoting growth of the T8-28 lymphoma cells.
authors.affiliation: University of Würzburg, Institute for Virology and Immunobiology, Würzburg, Germany. niklas.beyersdorf@vim.uni-wuerzburg.de
authors.forename: Niklas
authors.initials: N
authors.lastname: Beyersdorf
authors.forename: Sandra
authors.initials: S
authors.lastname: Werner
authors.forename: Nelli
authors.initials: N
authors.lastname: Wolf
authors.forename: Thomas
authors.initials: T
authors.lastname: Herrmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Kerkau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D018106:CD28 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D045744:Cell Line, Tumor; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D053804:Granzymes / Q000494:pharmacology; D006801:Humans; D016411:Lymphoma, T-Cell, Peripheral / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D016207:Cytokines; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D053804:Granzymes