title: Effects of matrine on proliferation and apoptosis in gallbladder carcinoma cells (GBC-SD).
abstract: Although matrine, a primary active component of dried Sophora flavescens root (ku shen), is known to induce apoptosis in a variety of tumor cells in vitro, the molecular mechanism of such apoptosis remains elusive. This analysis of the cell cycle and apoptosis in matrine-treated human gallbladder carcinoma cells (GBC-SD) showed that matrine can indeed inhibit cell proliferation and induce G1 cell cycle arrest and apoptosis in a dose- and time-dependent manner. An additional western blot analysis of matrine-treated cells also showed caspase-3 and Bcl-2 activation, as well as cyclinE down-regulation. Overall, the results indicate that matrine perturbs gallbladder cancer cell progression during the G1 phase by down-regulating cyclinE and induces apoptosis by decreasing the expression of the antiapoptotic protein Bcl-2 and increasing expression of the proapoptotic protein Bax.
authors.affiliation: Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Fei
authors.forename: Songgang
authors.initials: S
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D019927:Cyclin E / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004789:Enzyme Activation; D059585:G1 Phase Cell Cycle Checkpoints; D005706:Gallbladder Neoplasms / Q000473:pathology; D006801:Humans; D011807:Quinolizines / Q000494:pharmacology*; D029909:Sophora / Q000737:chemistry; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D019927:Cyclin E; D011807:Quinolizines; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; D053148:Caspase 3; D000093842:Matrines