title: Metallization of a genetically engineered polypeptide.
abstract: Recently, well-ordered biological materials have been exploited to pattern inorganic nanoparticles into linear arrays that are of particular interest for nanoelectronic applications. In this work, a de novo designed E. coli-expressed polypeptide (previously shown to form highly rectilinear, β-sheet-containing structures) operates as a template for divalent metal cations. EDX and TEM analysis verify the attachment of platinum ions to the histidine-rich fibril surface, which was designed specifically to facilitate attachment of chemical moieties. Following chemical reduction, TEM further confirms the formation of localized zero-valent metal aggregates with sub-nanometer interparticle spacing.
authors.affiliation: College of Nanoscale Science and Engineering (CNSE), University at Albany, SUNY, Albany, NY 12203, USA. atcarlsen@gmail.com
authors.forename: Autumn
authors.initials: A
authors.lastname: Carlsen
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Higashiya
authors.forename: Natasha I
authors.initials: NI
authors.lastname: Topilina
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Dunn
authors.forename: Robert E
authors.initials: RE
authors.lastname: Geer
authors.forename: Eric T
authors.initials: ET
authors.lastname: Eisenbraun
authors.forename: Alain E
authors.initials: AE
authors.lastname: Kaloyeros
authors.forename: John T
authors.initials: JT
authors.lastname: Welch
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000032:analysis / Q000138:chemical synthesis*; D002413:Cations, Divalent / Q000737:chemistry; D004926:Escherichia coli; D006639:Histidine / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D010984:Platinum / Q000737:chemistry*; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013052:Spectrometry, X-Ray Emission
chemical_list: D001672:Biocompatible Materials; D002413:Cations, Divalent; D010455:Peptides; D011994:Recombinant Proteins; D010984:Platinum; D006639:Histidine