title: In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase.
abstract: Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.
authors.affiliation: Department of Biochemistry, Hacettepe University, Ankara, Turkey.
authors.forename: Berivan
authors.initials: B
authors.lastname: Tandogan
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Güvenç
authors.forename: İhsan
authors.initials: İ
authors.lastname: Çalış
authors.forename: Nuriye Nuray
authors.initials: NN
authors.lastname: Ulusu
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology; D005980:Glutathione Reductase / Q000737:chemistry* / Q000302:isolation & purification; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry; D031365:Sideritis / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D047309:Flavones; D006027:Glycosides; D010636:Phenols; C000722867:isoscutellarein; D005980:Glutathione Reductase