title: Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase.
abstract: Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. MPKs can directly modulate gene expression by the phosphorylation of transcription factors. However, only a few target substrates of MPKs have been isolated. Here, we identified a C(2)H(2)-type zinc finger transcription factor from Arabidopsis, ZAT10, as a substrate of MPKs. Using in vitro and in vivo protein-protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and MPK6. ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. Furthermore, ZAT10 was also phosphorylated by native MPK3 and MPK6 prepared from Arabidopsis plants in an in-gel kinase assay. Mass spectrometry analysis of phosphopeptides was used to determine two MPK phosphorylation sites in ZAT10. These sites were verified by site-directed mutagenesis and in vitro kinase assays.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, 900 Gajwa, Room No. 6-320, Jinju 660-701, Republic of Korea.
authors.forename: Xuan Canh
authors.initials: XC
authors.lastname: Nguyen
authors.forename: Sun Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Lee
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Kim
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Liu
authors.forename: Hay Ju
authors.initials: HJ
authors.lastname: Han
authors.forename: My Hanh Thi
authors.initials: MH
authors.lastname: Hoang
authors.forename: Shin-Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Hong
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Moon
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010748:Phosphopeptides; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D011994:Recombinant Proteins; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; D020928:Mitogen-Activated Protein Kinases; D010748:Phosphopeptides; D011994:Recombinant Proteins; D014157:Transcription Factors; C516241:STZ protein, Arabidopsis; C458516:AtMPK3 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis