title: Production of multi-fiber modifying enzyme from Mamillisphaeria sp. for refining of recycled paper pulp.
abstract: Enzymatic modification of pulp is receiving increasing interest for energy reduction at the refining step of the paper-making process. In this study, the production of a multi-fiber modifying enzyme from Mamillisphaeria sp. BCC8893 was optimized in submerged fermentation using a response-surface methodology. Maximal production was obtained in a complex medium comprising wheat bran, soybean, and rice bran supplemented with yeast extract at pH 6.0 and a harvest time of 7 d, resulting in 9.2 IU/mL of carboxymethyl cellulase (CMCase), 14.9 IU/mL of filter paper activity (FPase), and 242.7 IU/mL of xylanase. Treatment of old corrugated container pulp at 0.2-0.3 IU of CMCase/g of pulp led to reductions in refining energy of 8.5-14.8%. The major physical properties were retained, including tensile and compression strength. Proteomic analysis showed that the enzyme was a complex composite of endo-glucanases, cellobiohydrolases, beta-1,4-xylanases, and beta-glucanases belonging to various glycosyl hydrolase families, suggestive of cooperative enzyme action in fiber modification, providing the basis for refining efficiency.
authors.affiliation: National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.
authors.forename: Thanaporn
authors.initials: T
authors.lastname: Laothanachareon
authors.forename: Parichart
authors.initials: P
authors.lastname: Khonzue
authors.forename: Nakul
authors.initials: N
authors.lastname: Rattanaphan
authors.forename: Phungjai
authors.initials: P
authors.lastname: Tinnasulanon
authors.forename: Saowanee
authors.initials: S
authors.lastname: Apawasin
authors.forename: Atchara
authors.initials: A
authors.lastname: Paemanee
authors.forename: Vasimon
authors.initials: V
authors.lastname: Ruanglek
authors.forename: Sutipa
authors.initials: S
authors.lastname: Tanapongpipat
authors.forename: Verawat
authors.initials: V
authors.lastname: Champreda
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000378:metabolism*; D005285:Fermentation; D055772:Green Chemistry Technology / Q000191:economics / Q000379:methods*; D007101:Immersion; D007221:Industry; D010209:Paper; D040901:Proteomics
chemical_list: D002480:Cellulase; C019826:carboxymethylcellulase; D043364:Endo-1,4-beta Xylanases