title: Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth.
abstract: Aneuploid is recognized as a hallmark of cancer and is caused by chromosome mis-segregation during mitosis. Recent studies have identified shugoshin (SGO-1) as an important player in proper chromosome segregation and the involvement in tumorigenesis. But, little is known about how SGO-1 is involved. The aim of this study was to obtain information about the role of SGO-1 in human lung cancer cells. In our study, real-time PCR and western blotting assay were performed to detect the expression of SGO-1 in nonsmall cell lung cancer (NSCLC) cell lines. Then, we successfully constructed a lentivirus vector mediating RNAi targeting of SGO-1 (LV-SGO-1 siRNA) and proved that it can suppress the expression of SGO-1 gene in H1299 cells. We found that SGO-1 was highly expressed in NSCLC cancer cell lines. RNA interference of SGO-1 by the LV-SGO-1 siRNA construct significantly decreased SGO-1 protein expression and inhibited the growth and ability of forming colonies of H1299 cells with more cells arrested in G2/M phase, but lentivirus vector control had no effect on H1299 cells. Moreover, suppression of SGO-1 by LV-SGO-1 siRNA increased the apoptosis of H1299 cells with down-regulated Bcl-2 and up-regulated Bax. SGO-1 may provide a novel therapeutic target for the treatment of lung cancer since it inhibits the growth and increases the apoptosis of lung cancer cells.
authors.affiliation: Department of Oncology, Tangdu Hospital, Fourth Military Medical University, 1 Xinsi Road, Xi'an, 710038, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Jiayu
authors.initials: J
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.forename: Ningqiang
authors.initials: N
authors.lastname: Ma
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Helong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000628:therapy; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000628:therapy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering; C496644:SGO1 protein, human