title: Proteome of the early embryo-maternal dialogue in the cattle uterus.
abstract: We analyzed embryo-maternal interactions in the bovine uterus on day 8 of development. Proteomic profiles were obtained by two-dimensional difference gel electrophoresis from 8 paired samples of uterine fluid (UF) from the same animal with and without embryos in the uterus. Results were contrasted with UF obtained after artificial insemination. We detected 50 differential protein spots (t test, p < 0.05). Subsequent protein characterization by nano-LC-ESI-MS/MS enabled us to identify 38 proteins, obtaining for first time the earliest evidence of involvement of the down-regulated NFkB system in cattle as a pregnancy signature pathway. Embryos enhanced the embryotrophic ability of UF and decreased uterine protein, while blood progesterone was unaltered. Twinfilin, hepatoma-derived growth factor, and synaptotagmin-binding cytoplasmic RNA interacting protein have not previously been identified in the mammalian uterus. TNFα and IL-1B were localized to embryos by immunocytochemistry, and other proteins were validated by Western blot in UF. Glycosylated-TNFα, IL-1B, insulin, lactotransferrin, nonphosphorylated-peroxiredoxin, albumin, purine nucleoside phosphorylase, HSPA5, and NFkB were down-regulated, while phosphorylated-peroxiredoxin, annexin A4, and nonglycosylated-TNFα were up-regulated. The embryonic signaling agents involved could be TNFα and IL-1B, either alone or in a collective dialogue with other proteins. Such molecules might explain the immune privilege during early bovine development.
authors.affiliation: Centro de Biotecnología Animal - SERIDA Camino de Rioseco , 1225 La Olla - Deva 33394 Gijón, Asturias, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Muñoz
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Corrales
authors.forename: José N
authors.initials: JN
authors.lastname: Caamaño
authors.forename: Carmen
authors.initials: C
authors.lastname: Díez
authors.forename: Beatriz
authors.initials: B
authors.lastname: Trigal
authors.forename: María I
authors.initials: MI
authors.lastname: Mora
authors.forename: David
authors.initials: D
authors.lastname: Martín
authors.forename: Susana
authors.initials: S
authors.lastname: Carrocera
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D015153:Blotting, Western; D001826:Body Fluids / Q000737:chemistry; D002417:Cattle / Q000196:embryology* / Q000378:metabolism*; D002452:Cell Count; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D011247:Pregnancy; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D020543:Proteome; D014409:Tumor Necrosis Factor-alpha