title: Bone turnover markers in peripheral blood and marrow plasma reflect trabecular bone loss but not endocortical expansion in aging mice.
abstract: We examined age-related changes in biochemical markers and regulators of osteoblast and osteoclast activity in C57BL/6 mice to assess their utility in explaining age-related changes in bone. Several recently discovered regulators of osteoclasts and osteoblasts were also measured to assess concordance between their systemic levels versus their levels in marrow plasma, to which bone cells are directly exposed. MicroCT of 6-, 12-, and 24-month-old mice indicated an early age-related loss of trabecular bone volume and surface, followed by endocortical bone loss and periosteal expansion. Trabecular bone loss temporally correlated with reductions in biomarkers of bone formation and resorption in both peripheral blood and bone marrow. Endocortical bone loss and periosteal bone gain were not reflected in these protein biomarkers, but were well correlated with increased expression of osteocalcin, rank, tracp5b, and cathepsinK in RNA extracted from cortical bone. While age-related changes in bone turnover markers remained concordant in blood versus marrow, aging led to divergent changes in blood versus marrow for the bone cell regulators RANKL, OPG, sclerostin, DKK1, and serotonin. Bone expression of runx2 and osterix increased progressively with aging and was associated with an increase in the number of osteoprogenitors and osteoclast precursors. In summary, levels of biochemical markers of bone turnover in blood and bone marrow plasma were predictive of an age-related loss of trabecular surfaces in adult C57BL/6 mice, but did not predict gains in cortical surfaces resulting from cortical expansion. Unlike these turnover markers, a panel of bone cell regulatory proteins exhibited divergent age-related changes in marrow versus peripheral blood, suggesting that their circulating levels may not reflect local levels to which osteoclasts and osteoblasts are directly exposed.
authors.affiliation: Division of Endocrinology, Veterans Affairs Medical Center, and Department of Medicine, University of California, San Francisco, San Francisco, CA 94121, USA. moshah@ucsf.edu
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shahnazari
authors.forename: Denise
authors.initials: D
authors.lastname: Dwyer
authors.forename: Vivian
authors.initials: V
authors.lastname: Chu
authors.forename: Frank
authors.initials: F
authors.lastname: Asuncion
authors.forename: Marina
authors.initials: M
authors.lastname: Stolina
authors.forename: Michael
authors.initials: M
authors.lastname: Ominsky
authors.forename: Paul
authors.initials: P
authors.lastname: Kostenuik
authors.forename: Bernard
authors.initials: B
authors.lastname: Halloran
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001853:Bone Marrow / Q000378:metabolism; D016723:Bone Remodeling / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism*; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D053244:Osteoprotegerin; D053245:RANK Ligand