title: Isolation and culture of astrocytes from the retina and optic nerve.
abstract: Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.
authors.affiliation: Molecular Pharmacology and Biological Chemistry Ward 8-200, Northwestern University, Chicago, IL, USA. t-lukas@northwestern.edu
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Lukas
authors.forename: Ai Ling
authors.initials: AL
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D004210:Dissection / Q000379:methods; D005434:Flow Cytometry; D006801:Humans; D009900:Optic Nerve / Q000166:cytology*; D051381:Rats; D012160:Retina / Q000166:cytology*