title: Transient global cerebral ischemia induces up-regulation of MLTKα in hippocampal CA1 neurons.
abstract: MLTK (mixed-lineage kinase-like mitogen-activated protein triple kinase) is a member of the mitogen-activated protein kinase family and functioned as a mitogen activated kinase kinase kinase. MLTKα, one of the alternatively spliced forms of MLTK, could activate the c-Jun N-terminal kinase pathway, which involved in cellular stress responses and apoptosis. But the role of MLTKα in neural apoptosis was still unclear. Here, we performed a transient global cerebral ischemia model (TGCI) in adult rats and detected the dynamic changes of MLTKα in hippocampal CA1 neurons and brain cortex. We found the MLTKα expression was increased shortly after TGCI and peaked after 8 h. In spatial distribution, MLTKα was widely located in neurons rather than astrocytes and microglia. Moreover, there was a concomitant up-regulation of active caspase-3. Taken together, we hypothesized the up-regulation of MLTKα played an essential role in the apoptosis of hippocampal CA1 neurons.
authors.affiliation: Department of Neurosurgery, Surgical Research Center, Hospital Affiliated to Nantong University, Xisi Road 20#, Nantong, 226001, Jiangsu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Su
authors.forename: Chang-Lai
authors.initials: CL
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Lan-Chun
authors.initials: LC
authors.lastname: Ni
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D002546:Ischemic Attack, Transient / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D020930:MAP Kinase Kinase Kinases / Q000235:genetics* / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D007527:Isoenzymes; D048031:JNK Mitogen-Activated Protein Kinases; D020930:MAP Kinase Kinase Kinases; C428384:MLK-like mitogen-activated protein triple kinase; D053148:Caspase 3