title: Molecular characterization of Bacillus thuringiensis isolated from diverse habitats of India.
abstract: Bacillus thuringiensis (Bt) strains were isolated from 94 samples from different geographical regions. Novel types of crystalline inclusion bodies were observed from some of the isolates. Crystalline inclusions of bipyramidal, spherical and cuboidal morphology were found produced by most of the isolates. Isolate GS12 showed crystal on one side of spore while isolate GM108 formed crystals on both termini of spore. Isolate GN31 produced large sized bipyramidal crystals. SDS-PAGE analysis of the spore crystal suspension showed major protein bands in the range of 29 and 140 kDa. Two new serovars of Bt viz. GS4 and GN24 having H3abce and H3ab serotype respectively were isolated. Toxicity comparable to the reference strain Bacillus thuringiensis subs. kurstaki (Btk) HD-1 was observed for the isolates GM20, GM17 and MP3 against larvae of Helicoverpa armigera. Some of the isolates harboring cry genes like cry1Ac and cry2 did not show any toxicity towards H. armigera while most of the isolates were harboring cry1, cry1Ac and cry2 gene.
authors.affiliation: Ground Floor Laboratory, Department of Microbiology and Biotechnology Center, The MS University of Baroda, Vadodara, Gujarat, India.
authors.forename: Ketan D
authors.initials: KD
authors.lastname: Patel
authors.forename: Chaitanyasinh J
authors.initials: CJ
authors.lastname: Chudasama
authors.forename: Sanjay S
authors.initials: SS
authors.lastname: Ingle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000166:cytology / Q000302:isolation & purification*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D002479:Inclusion Bodies; D007194:India; D007915:Lepidoptera / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis