title: Decrease in calcium-sensing receptor in the progress of diabetic cardiomyopathy.
abstract: To observe the dynamic expression of calcium-sensing receptor (CaSR) in myocardium of diabetic rats and explore its role in diabetic cardiomyopathy (DCM), 40 male Wistar rats were randomly divided into 4 groups including control, diabetic-4 weeks, diabetic-8 weeks and spermine treatment groups (240 μM of spermine in drinking water). The type 2 Diabetes mellitus (DM) models were established by intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) after high-fat and high-sugar diet for one month. The echocardiographic parameters were measured, cardiac morphology was observed by electron microscope and HE staining. The intracellular calcium concentration ([Ca(2+)](i)) was detected by laser-scanning confocal microscope. Western blot analyzed the expression of CaSR, protein kinase C α(PKC-α) and calcium handling regulators, such as phospholamban (PLN), Ca(2+)-ATPase (SERCA), and ryanodine receptor (RyR). Compared with control group, [Ca(2+)](i) and the expression of CaSR, RyR and SERCA/PLN were decreased, while PKC-α and PLN were significantly increased in a time-dependent manner in diabetic groups. Meanwhile diabetic rats displayed abnormal cardiac structure and systolic and diastolic dysfunction, and spermine (CaSR agonist) could prevent or slow its progression. These results indicate that the CaSR expression of myocardium is reduced in the progress of DCM, and its potential mechanism is related to the impaired intracellular calcium homeostasis.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
authors.forename: Shu-zhi
authors.initials: SZ
authors.lastname: Bai
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Li
authors.forename: Guang-wei
authors.initials: GW
authors.lastname: Li
authors.forename: Hong-zhu
authors.initials: HZ
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: He
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Zhang
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Li-na
authors.initials: LN
authors.lastname: Wang
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Yang
authors.forename: Guang-dong
authors.initials: GD
authors.lastname: Yang
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis; D003921:Diabetes Mellitus, Experimental; D058065:Diabetic Cardiomyopathies / Q000378:metabolism*; D006706:Homeostasis; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000032:analysis / Q000378:metabolism*; D013096:Spermine / Q000627:therapeutic use
chemical_list: D044169:Receptors, Calcium-Sensing; D013096:Spermine; D002118:Calcium