title: Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer.
abstract: Bacterial strains 2APBS1(T) and 116-2 were isolated from the subsurface of a nuclear legacy waste site where the sediments are co-contaminated with large amounts of acids, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first member of the genus Rhodanobacter shown to be capable of complete denitrification. Cells of strain 2APBS1(T) and 116-2 were Gram-negative, non-spore-forming rods, 3-5 µm long and 0.25-0.5 µm in diameter. The isolates were facultative anaerobes, and had temperature and pH optima for growth of 30 °C and pH 6.5; they were able to tolerate up to 2.0 % NaCl, although growth improved in its absence. Strains 2APBS1(T) and 116-2 contained fatty acid and quinone (ubiquinone-8; 100 %) profiles that are characteristic features of the genus Rhodanobacter. Although strains 2APBS1(T) and 116-2 shared high 16S rRNA gene sequence similarity with Rhodanobacter thiooxydans LCS2(T) (>99 %), levels of DNA-DNA relatedness between these strains were substantially below the 70 % threshold used to designate novel species. Thus, based on genotypic, phylogenetic, chemotaxonomic and physiological differences, strains 2APBS1(T) and 116-2 are considered to represent a single novel species of the genus Rhodanobacter, for which the name Rhodanobacter denitrificans sp. nov. is proposed. The type strain is 2APBS1(T) ( = DSM 23569(T) = JCM 17641(T)).
authors.affiliation: Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Om
authors.initials: O
authors.lastname: Prakash
authors.affiliation: DNA Services Facility, University of Illinois at Chicago, Chicago, IL, USA.|Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Stefan J
authors.initials: SJ
authors.lastname: Green
authors.affiliation: Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Puja
authors.initials: P
authors.lastname: Jasrotia
authors.affiliation: Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Will A
authors.initials: WA
authors.lastname: Overholt
authors.affiliation: Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Canion
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Watson
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Brooks
authors.affiliation: Earth, Ocean, and Atmospheric Science Department, Florida State University, Tallahassee, FL, USA.
authors.forename: Joel E
authors.initials: JE
authors.lastname: Kostka
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060587:Groundwater / Q000382:microbiology*; D008969:Molecular Sequence Data; D009566:Nitrates; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011850:Radioactive Waste; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014501:Uranium; D014877:Water Pollution, Chemical; D014878:Water Pollution, Radioactive; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D011850:Radioactive Waste; D014451:Ubiquinone; D014501:Uranium; C030778:ubiquinone 8