title: In vitro prevention of cataract by Oyster Mushroom Pleurotus florida extract on isolated goat eye lens.
abstract: OBJECTIVE
  The aim of the present work was to evaluate the in vitro effect of Pleurotus florida extract cataract induced by glucose.
  
  
  METHODS
  Goat eye lenses were divided into four groups. Group I lenses were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lenses were incubated with glucose concentration 55 mM (toxic control). Group III and IV lenses incubated with glucose concentration 55 mM were incubated along with hydroethanolic extract of P. florida 250 μg/ml and 500 μg/ml and subjected to morphological and biochemical evaluation.
  
  
  RESULTS
  Group II lenses showed high amount of malondialdehyde (MDA) soluble and insoluble protein and decreased catalase and glutathione levels, while lenses treated with P. florida extract showed significant (P < 0.05) reduction in MDA, increased level of catalase (P < 0.001), glutathione (P < 0.005) and total and soluble protein.
  
  
  CONCLUSIONS
  Hydroethanolic extract of P. florida showed prevention of in vitro glucose induced cataract. Thus, the goat lens model could be used for testing of various anticataract agents.
authors.affiliation: Department of Pharmacology, Shri Ram Institute of Technology-Pharmacy, Jabalpur, Madhya Pradesh, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Ganeshpurkar
authors.forename: Santosh Singh
authors.initials: SS
authors.lastname: Bhadoriya
authors.forename: Priya
authors.initials: P
authors.lastname: Pardhi
authors.forename: Alok Pal
authors.initials: AP
authors.lastname: Jain
authors.forename: Gopal
authors.initials: G
authors.lastname: Rai