title: Short communication: Colony-forming hematopoietic progenitor cells are not preferentially infected by HIV type 1 subtypes A and D in vivo.
abstract: HIV subtype C has previously been shown to infect hematopoietic progenitor cells (HPCs) at a significantly higher rate than subtype B. To better understand the subtype-specific nature of HPC infection, we examined the prevalence of HPC infection in vivo by HIV-1 subtypes A and D. HIV-1 infection of HPC was examined in 40 individuals, 19 infected with subtype A and 21 with subtype D, using a single colony assay format. DNA from 1177 extracted colonies was tested for integrated viral DNA of the p24 gene. Four colonies were found to be stably infected, three of 462 colonies (0.65%) from HIV-1A-infected individuals (1/19 individuals) and one of 715 colonies (0.14%) from HIV-1D-infected individuals (1/22 individuals). These rates of colony infection were comparable to the rates observed in PBMCs from the same subjects. Additionally, no correlation was observed between cell colony density and circulating viral load or proviral load. Our findings suggest that HIV-1 subtypes A and D do not preferentially infect colony-forming HPCs over mature HIV target cells in vivo.
authors.affiliation: Johns Hopkins Medical Institute, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Mullis
authors.forename: Amy E
authors.initials: AE
authors.lastname: Oliver
authors.forename: Leigh Anne
authors.initials: LA
authors.lastname: Eller
authors.forename: David
authors.initials: D
authors.lastname: Guwatudde
authors.forename: Amy C
authors.initials: AC
authors.lastname: Mueller
authors.forename: Michael A
authors.initials: MA
authors.lastname: Eller
authors.forename: Hannah
authors.initials: H
authors.lastname: Kibuuka
authors.forename: Merlin
authors.initials: M
authors.lastname: Robb
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Redd
mesh_terms: D000595:Amino Acid Sequence; D015704:CD4 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D004279:DNA, Viral; D005260:Female; D015497:HIV-1 / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000821:virology*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008969:Molecular Sequence Data; D019562:Viral Load / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D004279:DNA, Viral; D014764:Viral Proteins