title: Spectrometry researches on interaction and sonodynamic damage of riboflavin (RF) to bovine serum albumin (BSA).
abstract: In this paper, the riboflavin (RF) was used to study the interaction and sonodynamic damage to bovine serum albumin (BSA) by fluorescence and UV-vis spectroscopy. The results showed that the RF could efficiently bind to BSA in aqueous solution. Under ultrasonic irradiation, the RF could obviously damage the BSA. In addition, synchronous fluorescence spectroscopy revealed that the RF showed more accessible to tryptophan (Trp) residues than to tyrosine (Tyr) residues. Also, it damaged Trp residues more seriously than Tyr residues under ultrasonic irradiation. At last, the generation of reactive oxygen species (ROS) in sonodynamic process was estimated by the method of Oxidation-Extraction Spectrometry (OES). And then, several radical scavengers were used to determine the kind of ROS. It was found that at least the singlet oxygen ((1)O(2)) and hydroxyl radicals (*OH) were generated.
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, PR China.
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jushi
authors.initials: J
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Mingming
authors.initials: M
authors.lastname: Zou
authors.forename: Siyu
authors.initials: S
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Yumei
authors.initials: Y
authors.lastname: Kong
authors.forename: Lixin
authors.initials: L
authors.lastname: Xia
mesh_terms: D000818:Animals; D002417:Cattle; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000378:metabolism; D012256:Riboflavin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics
chemical_list: D017382:Reactive Oxygen Species; D012710:Serum Albumin, Bovine; D012256:Riboflavin