title: Impact of bacterial and fungal processes on 14C-hexadecane mineralisation in weathered hydrocarbon contaminated soil.
abstract: In this study, the impact of bacterial and fungal processes on (14)C-hexadecane mineralisation was investigated in weathered hydrocarbon contaminated soil. The extent of (14)C-hexadecane mineralisation varied depending on the bioremediation strategy employed. Under enhanced natural attenuation conditions, (14)C-hexadecane mineralisation after 98 days was 8.5 ± 3.7% compared to <1.2% without nitrogen and phosphorus additions. (14)C-hexadecane mineralisation was further enhanced through Tween 80 amendments (28.9 ± 2.4%) which also promoted the growth of a Phanerochaete chyrsosporium fungal mat. Although fungal growth in weathered hydrocarbon contaminated soil could be promoted through supplementing additional carbon sources (Tween 80, sawdust, compost, pea straw), fungal (14)C-hexadecane mineralisation was negligible when sodium azide was added to soil microcosms to inhibit bacterial activity. In contrast, when fungal activity was inhibited through nystatin additions, (14)C-hexadecane mineralisation ranged from 6.5 ± 0.2 to 35.8 ± 3.8% after 98 days depending on the supplied amendment. Bacteria inhibition with sodium azide resulted in a reduction in bacterial diversity (33-37%) compared to microcosms supplemented with nystatin or microcosms without inhibitory supplements. However, alkB bacterial groups were undetected in sodium azide supplemented microcosms, highlighting the important role of this bacterial group in (14)C-hexadecane mineralisation.
authors.affiliation: School of Biological Sciences, Flinders University, Adelaide, South Australia 5001, Australia.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Adetutu
authors.forename: Andy S
authors.initials: AS
authors.lastname: Ball
authors.forename: John
authors.initials: J
authors.lastname: Weber
authors.forename: Samuel
authors.initials: S
authors.lastname: Aleer
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Dandie
authors.forename: Albert L
authors.initials: AL
authors.lastname: Juhasz
mesh_terms: D000473:Alkanes / Q000737:chemistry*; D000704:Analysis of Variance; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002250:Carbon Radioisotopes / Q000378:metabolism*; D016000:Cluster Analysis; D009584:Nitrogen; D009761:Nystatin / Q000494:pharmacology; D010578:Petroleum / Q000032:analysis*; D020075:Phanerochaete / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010758:Phosphorus; D016133:Polymerase Chain Reaction; D011136:Polysorbates / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019810:Sodium Azide; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D000473:Alkanes; D002250:Carbon Radioisotopes; D010578:Petroleum; D011136:Polysorbates; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D009761:Nystatin; D010758:Phosphorus; D019810:Sodium Azide; C007932:n-hexadecane; D009584:Nitrogen