title: Gelatin-based anionic hydrogel as biocompatible substrate for human keratinocyte growth.
abstract: Ionic hydrogels are biocompatible candidates for skin tissue engineering. Two hydrogels synthesized by crosslinking gelatin with polylysine (positively charged HG1) or polyglutamic acid (negatively charged HG2) were tested using spontaneously immortalized human keratinocytes (HaCaT). HaCaT cells displayed higher adhesion and proliferation onto HG2, forming a continuous and stratified epithelium after 7 days. Moreover HaCaT cells grown onto HG2 showed a decreased Epilysin and Filaggrin expression, while transglutaminase-1 expression was increased. Those data indicate that human keratinocyte can form a stratified epithelium onto HG2 that could therefore be an useful tool for skin tissue engineering.
authors.affiliation: Research Centre for Biocompatibility and Tissue Engineering, Department of Experimental and Clinical Medicine, University of Eastern Piedmont A. Avogadro, Novara, Italy. filippo.reno@med.unipmn.it
authors.forename: Filippo
authors.initials: F
authors.lastname: Renò
authors.forename: Manuela
authors.initials: M
authors.lastname: Rizzi
authors.forename: Mario
authors.initials: M
authors.lastname: Cannas
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D004848:Epithelium / Q000378:metabolism; D000091344:Filaggrin Proteins; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D015603:Keratinocytes / Q000166:cytology*; D011107:Polylysine / Q000737:chemistry; D012867:Skin / Q000378:metabolism; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D003432:Cross-Linking Reagents; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D020100:Hydrogels; D011107:Polylysine; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin