title: [Antibody responses to leucine aminopeptidase in Schistosoma japonicum infection].
abstract: OBJECTIVE
  To investigate the antibody response to leucine aminopeptidase in the different stages of Schistosoma japonicum infection.
  
  
  METHODS
  The expression product of SjLAP was identified by Western blot and further purified by using nickel column. The IgG levels in the response to SjLAP in murine and porcine sera were detected by ELISA at different time points after the infection of S. japonicum.
  
  
  RESULTS
  SjLAP expressed by E. coli was recognized by anti-his monoclonal antibody and S. japonicum-infected mice sera by Western blot. The results of ELISA showed that IgG responses to SjLAP rose gradually and reached the peak at 4 weeks post-infection for pigs (P1) and 6 weeks post-infection for mice (P2). With the appearance of a large number of eggs in the tissue, SjLAP-specific IgG levels were significantly down-regulated ( P1 = 0. 0004, P2 = 0. 0001).
  
  
  CONCLUSIONS
  SjLAP originated from the adult worm might become a potential target for early diagnosis of schistosomiasis japonica.
authors.affiliation: Department of Pathogenic Biology, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Ji
authors.forename: Guan-Ling
authors.initials: GL
authors.lastname: Wu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000917:Antibody Formation; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007931:Leucyl Aminopeptidase / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000201:enzymology / Q000254:growth & development / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D007931:Leucyl Aminopeptidase