title: Heterologous protein expression by Lactococcus lactis.
abstract: This chapter describes the use of Lactococcus lactis as a safe and efficient cell factory to produce heterologous proteins of medical interest. The relevance of the use of this lactic acid bacterium (LAB) is that it is a noncolonizing, nonpathogenic microorganism that can be delivered in vivo at a mucosal level. The use of strains of L. lactis in clinical trials in humans to alleviate inflammatory bowel diseases has opened up the possibility of using this same LAB to target other diseases.Several crucial aspects are addressed in this chapter, such as the expression of heterologous protein, subcellular compartment into which the heterologous protein is located, and description of a standardized protocol to process samples in cell and cell-free fractions to detect the targeted protein expressed by L. lactis.
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
authors.forename: Julio
authors.initials: J
authors.lastname: Villatoro-Hernández
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Odila
authors.initials: O
authors.lastname: Saucedo-Cárdenas
authors.forename: Roberto
authors.initials: R
authors.lastname: Montes-de-Oca-Luna
mesh_terms: D015478:Antigens, Heterophile / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D018929:Cell Culture Techniques / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D018274:Electroporation / Q000379:methods; D013294:Lactococcus lactis / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D015478:Antigens, Heterophile; D011994:Recombinant Proteins