title: Performance of individual Helicobacter pylori antigens in the immunoblot-based detection of H. pylori infection.
abstract: To develop a specific line blot (LB) for supporting ELISA-based serodiagnosis of Helicobacter pylori infection, individual native/recombinant H. pylori antigens were evaluated with respect to their reactivity with both serum IgG and IgA from 156 dyspeptic screening patients (67% H. pylori positive). Of 13 antigens, HP0175, p17, and p19 revealed highest positive likelihood ratios for H. pylori-specific IgG (> 5.0) and were selected as LB substrates, in addition to the established virulence markers VacA and CagA. For validation, the LB was compared to a commercial whole-cell-lysate-based ELISA by parallel (re-)analysis of 156 screening sera, 22 sera from diabetes mellitus patients and 15 sera from follow-up patients after H. pylori eradication. In screening patients, the combined use of IgG ELISA and LB revealed a sensitivity, specificity, and accuracy of 94%, 81%, and 90%, respectively, whereas IgG ELISA alone exhibited a low specificity of 75%. In diabetic and follow-up patients, IgA ELISA exhibited high accuracy of 89% and 93%, respectively, whereas IgG detection was unreliable (accuracy < 80%). In conclusion, using HP0175, p17, p19, CagA, and VacA as LB substrates significantly improves the specificity of anti-H. pylori IgG analysis, providing a reliable tool for (1) confirmation/refutation of ELISA-based screening results and (2) assessment of the CagA/VacA status.
authors.affiliation: AZ Sint-Jan, Laboratory of Microbiology, Bruges, Belgium. Marijke.Reynders@azsintjan.be
authors.forename: Marijke B M L
authors.initials: MB
authors.lastname: Reynders
authors.forename: Veronique Yvette
authors.initials: VY
authors.lastname: Miendje Deyi
authors.forename: Hafid
authors.initials: H
authors.lastname: Dahma
authors.forename: Thomas
authors.initials: T
authors.lastname: Scheper
authors.forename: Merle
authors.initials: M
authors.lastname: Hanke
authors.forename: Marc
authors.initials: M
authors.lastname: Decolvenaer
authors.forename: Anne
authors.initials: A
authors.lastname: Dediste
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D016481:Helicobacter Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014774:Virulence / Q000276:immunology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G