title: Nuclear proteome analysis of benzo(a)pyrene-treated HeLa cells.
abstract: Previously, we employed a proteomics-based 2-D gel electrophoresis assay to show that exposure to 10μM benzo(a)pyrene (BaP) during a 24 h frame can lead to changes in nuclear protein expression and alternative splicing. To further expand our knowledge about the DNA damage response (DDR) induced by BaP, we investigated the nuclear protein expression profiles in HeLa cells treated with different concentrations of BaP (0.1, 1, and 10μM) using this proteomics-based 2-D gel electrophoresis assay. We found 125 differentially expressed proteins in BaP-treated cells compared to control cells. Among them, 79 (63.2%) were down-regulated, 46 (36.8%) were up-regulated; 8 showed changes in the 1μM and 10μM BaP-treated groups, 2 in the 0.1μM and 10μM BaP-treated groups, 4 in the 0.1μM and 1μM BaP-treated groups, and only one showed changes in all three groups. Fifty protein spots were chosen for liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, and of these, 39 were identified, including subunits of the 26S proteasome and Annexin A1. The functions of some identified proteins were further examined and the results showed that they might be involved in BaP-induced DDR. Taken together, these data indicate that proteomics is a valuable approach in the study of environmental chemical-host interactions, and the identified proteins could provide new leads for better understanding BaP-induced mutagenesis and carcinogenesis.
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Chunlan
authors.initials: C
authors.lastname: Yan
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Chen
authors.forename: Huanrong
authors.initials: H
authors.lastname: Li
authors.forename: Guanglin
authors.initials: G
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Penelope J
authors.initials: PJ
authors.lastname: Duerksen-Hughes
authors.forename: Xinqiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D017305:Annexin A1 / Q000032:analysis / Q000235:genetics; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D004187:Disaccharides; D005965:Glucuronates; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics; D020543:Proteome / Q000187:drug effects* / Q000235:genetics
chemical_list: D017305:Annexin A1; D004187:Disaccharides; D005965:Glucuronates; D016328:NF-kappa B; D009687:Nuclear Proteins; D020543:Proteome; D001564:Benzo(a)pyrene; C059554:O-(glucuronic acid 2-sulfate)-(1--4)-O-(2,5)-anhydromannitol 6-sulfate; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease