title: Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo.
abstract: The objective of the study was to determine the effect of carbon nanoparticles produced by different methods on the growth of brain tumor and the development of blood vessels. Glioblastoma multiforme cells were cultured on the chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor. Both types of nanoparticles significantly decreased tumor mass and volume, and vessel area. Quantitative real-time polymerase chain reaction analysis showed downregulated fibroblast growth factor-2 and vascular endothelial growth factor expression at the messenger ribonucleic acid level. The present results demonstrate antiangiogenic activity of carbon nanoparticles, making them potential factors for anticancer therapy.
authors.affiliation: Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wierzbicki
authors.forename: Piotr
authors.initials: P
authors.lastname: Orlowski
authors.forename: Anna
authors.initials: A
authors.lastname: Hotowy
authors.forename: Tomasz
authors.initials: T
authors.lastname: Niemiec
authors.forename: Maciej
authors.initials: M
authors.lastname: Szmidt
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Mitura
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
mesh_terms: D000704:Analysis of Variance; D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply* / Q000188:drug therapy* / Q000473:pathology; D002244:Carbon / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002642:Chick Embryo; D015536:Down-Regulation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000098:blood supply* / Q000188:drug therapy* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D002244:Carbon