title: Seleno-detergent MAD phasing of leukotriene C4 synthase in complex with dodecyl-β-D-selenomaltoside.
abstract: Dodecyl-β-D-selenomaltoside (SeDDM) is a seleno-detergent with a β-glycosidic seleno-ether in place of the ether moiety in dodecyl-β-D-maltoside. Seleno-detergents are candidates for heavy-atom agents in experimental phasing of membrane proteins in protein crystallography. Crystals of a nuclear membrane-embedded enzyme, leukotriene C(4) synthase (LTC(4)S), in complex with SeDDM were prepared and a multiwavelength anomalous diffraction (MAD) experiment was performed. The SeDDM in the LTC(4)S crystal exhibited sufficient anomalous diffraction for determination of the structure using MAD phasing.
authors.affiliation: Structural Biophysics Laboratory, RIKEN SPring-8 Center, Harima Institute, Sayo, Hyogo, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Saino
authors.forename: Hideo
authors.initials: H
authors.lastname: Ago
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ukita
authors.forename: Masashi
authors.initials: M
authors.lastname: Miyano
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods*; D004187:Disaccharides / Q000737:chemistry*; D005982:Glutathione Transferase / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008958:Models, Molecular; D017646:Organosilicon Compounds / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D004187:Disaccharides; D017646:Organosilicon Compounds; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase