title: Chemical screening and identification of high cordycepin containing cultured isolate(s) of medicinal Chinese caterpillar mushroom, Ophiocordyceps sinensis (Berk.) G.H. Sung et al.
abstract: Forty isolates of Ophiocordyceps sinensis collected from Himalayan alpine meadows of Uttarakhand, India, and cultivated on Jhangora (Echinochloa crusgalli) grains were screened to identify the isolate(s) of high cordycepin content. The cultured mycelia were extracted with 50% methanol-chloroform and analyzed by HPTLC using chloroform:methanol (6:1 v/v) as mobile phase and densitometry scanning at 263 nm. Cordycepin varied from 0.002% to 0.029% was detected in twenty-one isolates. Compared to natural O. sinensis (0.004%, 0.006%), cordycepin was determined to be enhanced in twelve cultured samples.
authors.affiliation: Chemistry Division, Forest Research Institute, P.O. New Forest, Dehra Dun 248 006, India.
authors.forename: V K
authors.initials: VK
authors.lastname: Varshney
authors.forename: Amit
authors.initials: A
authors.lastname: Pandey
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: Divyadhara
authors.initials: D
authors.lastname: Rathod
authors.forename: Pooja
authors.initials: P
authors.lastname: Kannojia
mesh_terms: D001685:Biological Factors / Q000032:analysis* / Q000302:isolation & purification; D046508:Culture Techniques; D003839:Deoxyadenosines / Q000032:analysis* / Q000302:isolation & purification; D055951:Functional Food / Q000032:analysis*; D006999:Hypocreales / Q000737:chemistry* / Q000254:growth & development; D007194:India; D025282:Mycelium / Q000737:chemistry* / Q000254:growth & development
chemical_list: D001685:Biological Factors; D003839:Deoxyadenosines; C058120:cordycepin