title: Establishment and characterization of a cell line, EH-GB2, derived from             hepatic metastasis of gallbladder cancer.
abstract: Gallbladder cancer is a fatal neoplasia with an extremely low survival rate.             Liver invasion and metastasis are the most common causes of death; however, the             metastatic mechanism is still unclear, and no effective treatment methods are             available. To provide comprehensive and profound approaches in investigating the             metastatic mechanism and treatment methods, new cell lines derived from liver             metastasis are urgently needed. A hepatic metastasis lesion was obtained from             a 65-year-old patient, and was treated using a primary culture method to establish             a novel gallbladder cancer cell line. Different in vitro/in vivo methods were             used to characterize the phenotypes of this cell line. The gallbladder cancer             cell line was named EH-GB2, with a roughly 48-h doubling time. The cell line represents             stronger colony formation and migration abilities than the control group. The             cells showed complicated chromosomal abnormalities. EH-GB2 cells showed epithelial-to-mesenchymal             transition (EMT) and the mRNA expression levels of E-cadherin and integrin were             decreased, and those of vimentin, Snail, Twist, matrix metalloproteinase-1 (MMP-1)             and MMP-2 were increased in comparison with control cells. The in vivo study demonstrated             that EH-GB2 cells show significant tumorigenicity in nude mice. The EH-GB2 established             gallbladder cancer cell line is useful for future studies of gallbladder cancer             development, progression, metastasis and therapy.
authors.affiliation: Department of First Biliary Surgery, Eastern Hepatobiliary Surgical Hospital and Institute, The Second Military University, Shanghai 200438, PR China.
authors.forename: Jing-Han
authors.initials: JH
authors.lastname: Wang
authors.forename: Lin-Fang
authors.initials: LF
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Jin
authors.forename: Dong-Hao
authors.initials: DH
authors.lastname: Shen
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Qi-Jun
authors.initials: QJ
authors.lastname: Qian
mesh_terms: D000368:Aged; D000818:Animals; D015820:Cadherins / Q000235:genetics; D015197:Carcinogenicity Tests / Q000379:methods; D045744:Cell Line, Tumor / Q000473:pathology*; D002465:Cell Movement / Q000235:genetics; D005706:Gallbladder Neoplasms / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016023:Integrins / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D012908:Snails / Q000235:genetics; D051879:Twist-Related Protein 1 / Q000235:genetics; D014746:Vimentin / Q000235:genetics
chemical_list: D015820:Cadherins; D016023:Integrins; D012333:RNA, Messenger; D051879:Twist-Related Protein 1; D014746:Vimentin; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1