title: Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F2α synthase, AKR1B3.
abstract: Aldo-keto reductase 1B3 (AKR1B3) catalyzes the NADPH-dependent reduction of prostaglandin H(2) (PGH(2)), which is a common intermediate of various prostanoids, to form PGF(2α). AKR1B3 also reduces PGH(2) to PGD(2) in the absence of NADPH. AKR1B3 produced in Escherichia coli was crystallized in complex with NADPH by the sitting-drop vapour-diffusion method. The crystal was tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 107.62, c = 120.76 Å. X-ray diffraction data were collected to 2.4 Å resolution at 100 K using a synchrotron-radiation source.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Takashima
authors.forename: Seika
authors.initials: S
authors.lastname: Hatanaka
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Nanae
authors.initials: N
authors.lastname: Nagata
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Fukunishi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D006911:Hydroxyprostaglandin Dehydrogenases / Q000737:chemistry*; D051379:Mice
chemical_list: D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase