title: Development of a mass sensitive quartz crystal microbalance (QCM)-based DNA biosensor using a 50 MHz electronic oscillator circuit.
abstract: This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
authors.affiliation: Dpto Tecnología Electrónica, Universidad de Vigo, Campus Lagoas Marcosende, Vigo 36310, Spain. skevere@gmail.com
authors.forename: Gonzalo
authors.initials: G
authors.lastname: García-Martinez
authors.forename: Enrique Alonso
authors.initials: EA
authors.lastname: Bustabad
authors.forename: Hubert
authors.initials: H
authors.lastname: Perrot
authors.forename: Claude
authors.initials: C
authors.lastname: Gabrielli
authors.forename: Bogdan
authors.initials: B
authors.lastname: Bucur
authors.forename: Mathieu
authors.initials: M
authors.lastname: Lazerges
authors.forename: Daniel
authors.initials: D
authors.lastname: Rose
authors.forename: Loreto
authors.initials: L
authors.lastname: Rodriguez-Pardo
authors.forename: Jose
authors.initials: J
authors.lastname: Fariña
authors.forename: Chantal
authors.initials: C
authors.lastname: Compère
authors.forename: Antonio Arnau
authors.initials: AA
authors.lastname: Vives
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002138:Calibration; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004581:Electronics; D004867:Equipment Design; D009991:Oscillometry / Q000379:methods; D010825:Physics / Q000379:methods; D058525:Quartz Crystal Microbalance Techniques; D015203:Reproducibility of Results
chemical_list: D004220:Disulfides; D004247:DNA