title: Training increases peroxiredoxin 2 contents in the erythrocytes of overweight/obese men suffering from type 2 diabetes.
abstract: Type 2 diabetes mellitus (T2DM) is associated with an increased release of free radicals which play an important role in the manifestation of diabetes and in the progression of diabetic complications. Peroxiredoxins are thought to be essential components of the erythrocyte antioxidative defense. Therefore, we compared peroxiredoxin isoform contents (PRDX1-6 immuno-histochemial stainings) in the erythrocytes of overweight/obese T2DM men (n = 6) and of BMI-matched non-diabetic male control subjects (n = 6). Only erythrocyte PRDX1 and PRDX2 proteins were detectable using immunohistochemical methods. PRDX1 was significantly increased in T2DM men relative to control subjects (+95.9%, P ≤ 0.05). Furthermore, we studied the influence of a 3-month endurance training program (3 times a week, cycling at 75% maximal heart rate) on erythrocyte PRDX1 and PRDX2 contents in overweight/obese T2DM men (n = 11). Training significantly increased PRDX2 at rest (+96%, P ≤ 0.05). The up-regulation of the peroxiredoxin system may help counteract free radicals in the erythrocytes of T2DM patients.
authors.affiliation: Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany.
authors.forename: David Assadi
authors.initials: DA
authors.lastname: Moghaddam
authors.forename: Anke
authors.initials: A
authors.lastname: Heber
authors.forename: Dario
authors.initials: D
authors.lastname: Capin
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kreutz
authors.forename: David
authors.initials: D
authors.lastname: Opitz
authors.forename: Edward
authors.initials: E
authors.lastname: Lenzen
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
authors.forename: Klara
authors.initials: K
authors.lastname: Brixius
authors.forename: Christian
authors.initials: C
authors.lastname: Brinkmann
mesh_terms: D000328:Adult; D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000534:rehabilitation*; D004912:Erythrocytes / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005260:Female; D005609:Free Radicals / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000534:rehabilitation*; D050177:Overweight / Q000097:blood* / Q000534:rehabilitation*; D018384:Oxidative Stress / Q000502:physiology; D054464:Peroxiredoxins / Q000097:blood*
chemical_list: D005609:Free Radicals; C086065:PRDX2 protein, human; D054464:Peroxiredoxins