title: Addressing diffuse glioma as a systemic brain disease with single-cell analysis.
abstract: OBJECTIVE
  To analyze infiltration patterns of IDH1 mutant diffuse gliomas into the brain by identification of single tumor cells applying an antibody specific to mutant IDH1 R132H protein.
  
  
  METHODS
  Immunohistochemical analysis.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Whole-brain and hemisphere sections of 4 patients diagnosed with diffuse glioma.
  
  
  RESULTS
  Tumor cells were identified in areas that appeared inconspicuous macroscopically and at histological analysis with respect to cellularity, cellular pleomorphism, or mitotic activity in all cases.
  
  
  CONCLUSIONS
  Detection of single tumor cells throughout the brain demonstrates diffuse glioma to represent systemic brain disease.
authors.affiliation: Department of Neuropathology, Institute of Pathology, German Cancer Research Center, Heidelberg, Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Sahm
authors.forename: David
authors.initials: D
authors.lastname: Capper
authors.forename: Astrid
authors.initials: A
authors.lastname: Jeibmann
authors.forename: Antje
authors.initials: A
authors.lastname: Habel
authors.forename: Werner
authors.initials: W
authors.lastname: Paulus
authors.forename: Dirk
authors.initials: D
authors.lastname: Troost
authors.forename: Andreas
authors.initials: A
authors.lastname: von Deimling
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D007839:Functional Laterality; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D008279:Magnetic Resonance Imaging; D008297:Male; D009154:Mutation / Q000235:genetics*; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human