title: MicroRNA-205 functions as a tumor suppressor in human glioblastoma cells             by targeting VEGF-A.
abstract: MicroRNAs (miRNAs) are endogenously small non-coding RNAs which are key             post-transcriptional regulators of gene expression. Deregulation of miRNAs is             common in human tumorigenesis. We report that miRNA-205 is significantly down-regulated             in glioma cell lines and tissue specimens. Ectopic expression of miRNA-205 induces             apoptosis, cell cycle arrest, impairs cell viability, clonability and invasive             properties of glioma cells. We further demonstrate that miRNA-205 can specifically             suppress expression of VEGF-A by directly interacting with the putative miRNA-205             binding site at the 3'-UTR. Identification of VEGF-A as a direct target for miRNA-205             may imply that miRNA-205 is a novel target for glioma therapy. Taken together,             the present study for the first time provides evidence that miRNA-205 is a glioma-specific             tumor suppressor by targeting VEGF-A.
authors.affiliation: Department of Neurosurgery, Shandong Cancer Hospital, Shandong Academy of Medical Sciences, Jinan 250117, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yue
authors.forename: Peiguo
authors.initials: P
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.forename: Qi
authors.initials: Q
authors.lastname: Pang
authors.forename: Rongjie
authors.initials: R
authors.lastname: Tao
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D001665:Binding Sites; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D013997:Time Factors; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; C534425:MIR205, human; C467484:VEGFA protein, human