title: Molecular identification of a polyM-specific alginate lyase from  Pseudomonas  sp. strain KS-408 for degradation of glycosidic linkages between two mannuronates or mannuronate and guluronate in alginate.
abstract: An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingomonas sp. strain A1. The proposed 3-D structure of KS-408 alginate lyase shows that Asn-198, His-199, Arg-246, and Tyr-253 residues are conserved for the catalytic active site. The recombinant KS-408-1F (with signal peptide) and KS-408-2F (without signal peptide) alginate lyases with the (His)(6) tag consist of 393 (44.5 kDa) and 372 (42.4 kDa) amino acids with isoelectric points of 8.64 and 8.46, respectively. The purified recombinant KS-408 alginate lyase was very stable when it was incubated at 40 °C for 30 min. Alginate oligosaccharides produced by the KS-408-2F alginate lyase were purified on a Bio-Gel P2 column and analyzed by thin-layer chromatography, fast-protein liquid chromatography, and electrospray ionization mass spectrometry. (1)H NMR data showed that the KS-408-2F alginate lyase cleaved the glycosidic linkages between two mannuronates (mannuronate-β(1-4)-mannuronate) or mannuronate and guluronate (mannuronate-β(1-4)-guluronate), indicating that the KS-408 alginate lyase is a polyM-specific lyase.
authors.affiliation: Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Korea.
authors.forename: Natania
authors.initials: N
authors.lastname: Kam
authors.forename: Yoo Jung
authors.initials: YJ
authors.lastname: Park
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
authors.forename: Hee Sook
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D020578:Sphingomonas / Q000201:enzymology / Q000235:genetics
chemical_list: D000464:Alginates; D006027:Glycosides; D006603:Hexuronic Acids; D008670:Metals; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; C007896:guluronic acid; D020723:Glucuronic Acid; C008324:mannuronic acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase