title: Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).
abstract: Reverse transcription loop-mediated isothermal amplification (RT-LAMP), which is a visual assay for nucleic acids, is performed in a single step using one tube at 65 °C for 1.5 h. In this study, RT-LAMP was established as a method for the detection of enterovirus 71 (EV71). The detection limit of the assay was approximately 10 copies, and no cross-reactivity was noted with Coxsackievirus A16, echovirus, human rotavirus (HRV) or norovirus. This assay, which offers greater sensitivity at a lower cost compared with the conventional reverse transcription polymerase chain reaction (RT-PCR), was validated using 252 clinical specimens that had been confirmed by laboratory diagnosis using RT-PCR. Both methods produced the same results with 52 positive samples. The RT-LAMP-based assay does not require specialised equipment, and therefore, it can be performed conveniently during an outbreak or under field conditions. In brief, the RT-LAMP-based assay provided a simple, rapid and efficient method for the detection of EV71 nucleic acid under field conditions.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jun-ping
authors.initials: JP
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Wen-zhi
authors.initials: WZ
authors.lastname: Zheng
authors.forename: Li-shu
authors.initials: LS
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D029821:Enterovirus A, Human / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D007223:Infant; D025202:Molecular Diagnostic Techniques / Q000191:economics / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D014773:Virology / Q000191:economics / Q000379:methods*