title: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes.
abstract: We investigated the lateral diffusion of the HIV receptor CD4 at the surface of T lymphocytes at 20°C and 37°C by Single Particle Tracking using Quantum Dots. We found that the receptors presented two major distinct behaviors that were not equally affected by temperature changes. About half of the receptors showed a random diffusion with a diffusion coefficient increasing upon raising the temperature. The other half of the receptors was permanently or transiently confined with unchanged dynamics on raising the temperature. These observations suggest that two distinct subpopulations of CD4 receptors with different environments are present at the surface of living T lymphocytes.
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Mascalchi
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Lamort
authors.forename: Laurence
authors.initials: L
authors.lastname: Salomé
authors.forename: Fabrice
authors.initials: F
authors.lastname: Dumas
mesh_terms: D015704:CD4 Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry* / Q000276:immunology; D006678:HIV / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology
chemical_list: D015704:CD4 Antigens