title: Evaluation of indirect TaSP enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in Egypt.
abstract: The aim of the present study was to evaluate the validity of Theileria annulata surface protein (TaSP)-ELISA, in comparison with traditional microscopic test, for the diagnosis of T. annulata infection among Egyptian baladi cattle (Bos taurus) and water buffaloes (Bubalus bubalis). Molecular confirmation of infection using T. annulata merozoite surface (Tams-1) target amplification by PCR was used as a gold standard. A total of 76 clinically suspected animals including 64 baladi cattle and 12 water buffaloes were investigated in the current study by the three methods. Based on the PCR-confirmed results, the evaluation study revealed higher sensitivity of TaSP-ELISA (72.9% and 75%) as compared to microscopic examination (58.3% and 50%) among cattle and buffaloes, respectively. On the other hand, the specificity of TaSP-ELISA in diagnosis of T. annulata infection was higher (87.5%) in baladi cattle as compared to water buffaloes (37.5%). In conclusion, TaSP-ELISA was shown to be suitable for the diagnosis of T. annulata infection in cattle under field conditions.
authors.affiliation: Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71515, Egypt. amrmohamed2004@yahoo.com
authors.forename: Amr M
authors.initials: AM
authors.lastname: Mohamed
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdel-Rady
authors.forename: Laila S
authors.initials: LS
authors.lastname: Ahmed
authors.forename: Amira
authors.initials: A
authors.lastname: El-Hosary
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000097:blood / Q000469:parasitology*; D002417:Cattle; D004534:Egypt / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D008565:Membrane Proteins / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D016796:Theileria annulata / Q000302:isolation & purification*; D013801:Theileriasis / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D008565:Membrane Proteins