title: Controlled gene expression in primary Lgr5 organoid cultures.
abstract: The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.
authors.affiliation: Hubrecht Institute for Developmental Biology and Stem Cell Research, and University Medical Centre, Utrecht, The Netherlands.
authors.forename: Bon-Kyoung
authors.initials: BK
authors.lastname: Koo
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Stange
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sato
authors.forename: Wouter
authors.initials: W
authors.lastname: Karthaus
authors.forename: Henner F
authors.initials: HF
authors.lastname: Farin
authors.forename: Meritxell
authors.initials: M
authors.lastname: Huch
authors.forename: Johan H
authors.initials: JH
authors.lastname: van Es
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
mesh_terms: D000818:Animals; D015870:Gene Expression; D055785:Gene Knockdown Techniques / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D019426:Integrases / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D051379:Mice; D009940:Organoids / Q000166:cytology* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*; D051880:Receptors, Notch / Q000502:physiology; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells / Q000821:virology; D013629:Tamoxifen / Q000031:analogs & derivatives / Q000494:pharmacology; D014161:Transduction, Genetic / Q000379:methods
chemical_list: C493597:Lgr5 protein, mouse; D043562:Receptors, G-Protein-Coupled; D051880:Receptors, Notch; C485184:enhanced green fluorescent protein; D013629:Tamoxifen; D049452:Green Fluorescent Proteins; C016601:afimoxifene; C045073:Cre recombinase; D019426:Integrases