title: Selection and characterization of PCB-binding DNA aptamers.
abstract: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that resist natural degradation and bioaccumulate in nature. Combined with their toxicity, this leads them to cause cancer and other health hazards. Thus, there is a vital need for rapid and sensitive methods to detect PCB residues in food and in the environment. In this study, PCB-binding DNA aptamers were developed using PCB72 and PCB106 as targets for aptamer selection. Aptamers are synthetic DNA recognition elements which form unique conformations that enable them to bind specifically to their targets. Using in vitro selection techniques and fluorometry, an aptamer that binds with nanomolar affinity to both the PCBs has been developed. It displayed high selectivity to the original target congeners and limited affinity toward other PCB congeners (105, 118, 153, and 169), suggesting general specificity for the basic PCB skeleton with varying affinities for different congeners. This aptamer provides a basis for constructing an affordable, sensitive, and high-throughput assay for the detection of PCBs in food and environmental samples and offers a promising alternative to existing methods of PCB quantitation. This study therefore advances aptamer technology by targeting one of the highly sought-after POPs, for the first time ever recorded.
authors.affiliation: Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. Jaytry.Mehta@ua.ac.be
authors.forename: Jaytry
authors.initials: J
authors.lastname: Mehta
authors.forename: Elsa
authors.initials: E
authors.lastname: Rouah-Martin
authors.forename: Bieke
authors.initials: B
authors.lastname: Van Dorst
authors.forename: Bert
authors.initials: B
authors.lastname: Maes
authors.forename: Wouter
authors.initials: W
authors.lastname: Herrebout
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Scippo
authors.forename: Freddy
authors.initials: F
authors.lastname: Dardenne
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.forename: Johan
authors.initials: J
authors.lastname: Robbens
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D008280:Magnetics; D011078:Polychlorinated Biphenyls / Q000737:chemistry*; D017422:Sequence Analysis, DNA
chemical_list: D052157:Aptamers, Nucleotide; D017931:DNA Primers; D004247:DNA; D011078:Polychlorinated Biphenyls