title: Isolation and purification of oridonin from the whole plant of Isodon rubescens by high-speed counter-current chromatography.
abstract: Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.
authors.affiliation: College of Pharmacy, Harbin University, Daqing 163319, China.
authors.forename: Fa
authors.initials: F
authors.lastname: He
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Bai
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Chunyue
authors.initials: C
authors.lastname: Yu
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.forename: Weili
authors.initials: W
authors.lastname: Yang
authors.forename: Chenghua
authors.initials: C
authors.lastname: Han
mesh_terms: D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D045786:Diterpenes, Kaurane / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D037602:Isodon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C011959:oridonin