title: Bioadhesiveness and efficient mechanotransduction stimuli synergistically provided by bacterial inclusion bodies as scaffolds for tissue engineering.
abstract: BACKGROUND
  Bacterial inclusion bodies (IBs), mechanically stable, submicron protein particles of 50-500 nm dramatically favor mammalian cell spread when used for substrate surface decoration. The mechanisms supporting fast colonization of IB-modified surfaces have not yet been identified.
  
  
  RESULTS
  This study provides evidence of mechanotransduction-mediated stimulation of mammalian cell proliferation on IB-decorated surfaces, as observed by the enhanced phosphorylation of the signal-regulated protein kinase and by the dramatic emission of filopodia in the presence of IBs. Interestingly, the results also show that IBs are highly bioadhesive materials, and that mammalian cell expansion on IBs is synergistically supported by both enhanced adhesion and mechanical stimulation of cell division.
  
  
  CONCLUSIONS
  The extent in which these events influence cell growth depends on the particular cell line response but it is also determined by the genetic background of the IB-producing bacteria, thus opening exciting possibilities for the fine tailoring of protein nanoparticle features that are relevant in tissue engineering.
authors.affiliation: Institute for Biotechnology & Biomedicine, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Seras-Franzoso
authors.forename: César
authors.initials: C
authors.lastname: Díez-Gil
authors.forename: Esther
authors.initials: E
authors.lastname: Vazquez
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Rafael
authors.initials: R
authors.lastname: Cubarsi
authors.forename: Imma
authors.initials: I
authors.lastname: Ratera
authors.forename: Jaume
authors.initials: J
authors.lastname: Veciana
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D006224:Cricetinae; D004926:Escherichia coli / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D036103:Nanotechnology / Q000379:methods; D010766:Phosphorylation; D011554:Pseudopodia / Q000378:metabolism*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000382:microbiology*
chemical_list: D001672:Biocompatible Materials; D048049:Extracellular Signal-Regulated MAP Kinases