title: [The first experience of application of PCR techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients].
abstract: The results comparison is made between PCR polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. From September to December 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. The blood samples for PCR analysis (SeptiFast technique Roche diagnostics, Switzerland) were taken parallel to the inoculation. Among 43 samples 20 (46.5%) were positive. The species structure of identified germs was as follows: Enteroccocus Faecalis - 2, Klebsiella pneumonia - 2, Enteroccocusfaecium - 1, Pseudomonas aeruginosa - 2, coagulase negative Staphilococcus - 11. All cases of seeding of coagulase negative Staphilococcus were interpreted using PCR techniques as negative or contamination. In four cases a full matching of results of compared techniques was obtained. In cases when hemoculture was negative (n = 23) the PCR techniques permitted to detect two positive results (Staphilococcus spp. and Stenotrophomonas maltophilia). In three cases of positive hemoculture the PCR techniques brought negative result and at that in two cases the causative agents (Pseudomonas fluorescens and Ralstonia mannitolilytica) were out of specter of pathogens detected by this method. The PCR techniques in real-time mode permit to reduce significantly time of pathogen investigation and to detect the false positive results conditioned by contamination. This techniques has to be applied in parallel with cultural technique because only this approach provide a possibility to get the clinically needed data related to the sensitivity of causative agent to antibacterial preparations.
authors.forename: D A
authors.initials: DA
authors.lastname: Popov
authors.forename: T Iu
authors.initials: TIu
authors.lastname: Vostrikova
mesh_terms: D000368:Aged; D016470:Bacteremia / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D003030:Coagulase; D016983:Enterococcus / Q000302:isolation & purification; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011183:Postoperative Complications / Q000097:blood*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000302:isolation & purification; D020615:Stenotrophomonas maltophilia / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000302:isolation & purification; D013903:Thoracic Surgery
chemical_list: D003030:Coagulase