title: Effect of Rta protein of Epstein-Barr virus on the cell cycle in HeLa cells.
abstract: Epstein-Barr virus (EBV) replication and transcription activator (Rta) is an immediate-early transcription factor that mediates the switch from latent to lytic infection. DNA viruses often modulate the function of critical cell cycle proteins to maximize the efficiency of virus replication. Here we have examined the effect of Rta on cell cycle progression. Cell cycle analysis revealed that Rta induced HeLa cells in G0/G1-phase to reenter the S-phase. Analysis of the expression pattern of a key set of cell cycle regulators revealed that expression of Rta inhibited the expression of Rb and p53 and induced the expression of E2F1. These findings suggest that Rta plays an active role in redirecting HeLa cell physiology through an Rta-mediated cell cycle transformation.
authors.affiliation: Qilu Children's Hospital of Shandong University, Jinan, PR China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Guo
authors.forename: Xiaobai
authors.initials: X
authors.lastname: Sun
authors.forename: Changjin
authors.initials: C
authors.lastname: Yuan
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhou
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Li
authors.forename: Gao
authors.initials: G
authors.lastname: Jie
authors.forename: Guosheng
authors.initials: G
authors.lastname: Jiang
mesh_terms: D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D002472:Cell Transformation, Viral; D050687:E2F1 Transcription Factor / Q000378:metabolism; D016193:G1 Phase / Q000187:drug effects / Q000502:physiology; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D016160:Retinoblastoma Protein / Q000037:antagonists & inhibitors; D016196:S Phase / Q000187:drug effects / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors; D015854:Up-Regulation
chemical_list: C068382:BRLF1 protein, Human herpesvirus 4; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D017874:Immediate-Early Proteins; D016160:Retinoblastoma Protein; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53