title: Microspread ovarian cell preparations for the analysis of meiotic prophase progression in oocytes with improved recovery by cytospin centrifugation.
abstract: Female fertility is critically influenced by two events affecting oocytes during meiotic prophase progression: meiotic recombination between homologous chromosomes; and a major oocyte loss. It is technically challenging to examine these events, which take place in fetal and neonatal ovaries in mammals. Here, we describe a protocol for the preparation of dissociated ovarian cells and their spread onto histology slides. These preparations are suitable for cytogenetic, quantitative, and FISH analyses.
authors.affiliation: Department of Surgery, McGill University, Royal Victoria Hospital, Montreal, QC, Canada. teruko.taketo@mcgill.ca
authors.forename: Teruko
authors.initials: T
authors.lastname: Taketo
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D005260:Female; D049469:Meiotic Prophase I / Q000502:physiology*; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D010053:Ovary / Q000166:cytology*