title: Inhibition of nuclear factor kappa B activation reduces Coxsackievirus B3 replication in lymphoid cells.
abstract: Interactions between viral replication machineries and host cell metabolism display interesting information how certain viruses capitalize cellular pathways to support progeny production. Among those pathogens, Coxsackievirus B3 (CVB3) has been identified to manipulate intracellular signaling very comprehensively. Next to others, this human pathogenic virus causes acute and chronic forms of myocarditis, pancreatitis, and meningitis. Here, activation of nuclear factor kappa B (NFκB) signaling appears to be involved in successful infection. Viral replication is not restricted to solid organs but involves susceptible immune cells as well. In the present study, p65 phosphorylation as one aspect of NFκB activation and inhibition via BAY 11-7085 administration was analyzed in the context of CVB3 replication in lymphoid cells. During CVB3 infection, an up-regulation of p65 translation is detectable, which is accompanied by noticeable phosphorylation. Inhibition of NFκB signaling reduces viral replication in a dose- and time-dependent manner. Taken together, these results indicate that during CVB3 replication in human and murine lymphoid cells, NFκB signaling is activated and facilitates viral replication. Therefore, antiviral strategies to target such central cellular signaling pathways may represent potential possibilities for the development of new virostatica.
authors.affiliation: Department of Virology and Antiviral Therapy, Jena University Hospital, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Sobotta
authors.forename: Steffi
authors.initials: S
authors.lastname: Wilsky
authors.forename: Nadine
authors.initials: N
authors.lastname: Althof
authors.forename: Nadine
authors.initials: N
authors.lastname: Wiesener
authors.forename: Peter
authors.initials: P
authors.lastname: Wutzler
authors.forename: Andreas
authors.initials: A
authors.lastname: Henke
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000254:growth & development*; D054884:Host-Pathogen Interactions; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*
chemical_list: D016328:NF-kappa B