title: Expression of IL-1Rrp2 by human myelomonocytic cells is unique to DCs and facilitates DC maturation by IL-1F8 and IL-1F9.
abstract: We report for the first time that expression of the novel IL-1 cytokine receptor IL-1Rrp2 (IL-1R6) is unique to DCs within the human myelomonocytic lineage. IL-1Rrp2 was expressed by monocyte-derived dendritic cells (MDDCs) which was dose-dependently increased by IL-4 and correlated with increased numbers of differentiated MDDCs. Human plasmacytoid DCs also express IL-1Rrp2 but the receptor is not expressed by either myeloid DC type 1 (mDC1) or mDC2 cells. We also show that IL-1F8 or IL-1F9 cytokines, which signal through IL-1Rrp2, induce maturation of MDDCs, as measured by increased expression of HLA-DR and CD83 and decreased expression of CD1a. Furthermore, IL-1F8 stimulated increased CD40 and CD80 expression and IL-18 and IL-12 p70 production by MDDCs, which induced proliferation of IFN-γ-producing CD3(+)  lymphocytes (indicative of inflammatory Th1 subsets). IL-1F8 and IL-1F2 were equipotent in their ability to stimulate IL-18 secretion from MDDCs but IL-1F8 was not as potent as IL-1F2 in stimulating secretion of IL-12p70 from MDDCs or inducing lymphocyte proliferation Therefore, IL-1Rrp2 expression by some DC subsets may have an important function in the human immune response in vivo via its role in differentiation of inflammatory Th1 lymphocytes.
authors.affiliation: School of Veterinary Medicine and Science,  University of Nottingham, UK.
authors.forename: Shilla
authors.initials: S
authors.lastname: Mutamba
authors.forename: Asher
authors.initials: A
authors.lastname: Allison
authors.forename: Yashwant
authors.initials: Y
authors.lastname: Mahida
authors.forename: Paul
authors.initials: P
authors.lastname: Barrow
authors.forename: Neil
authors.initials: N
authors.lastname: Foster
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D018949:Antigens, CD1 / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology*; D053727:Interleukin-18 Receptor alpha Subunit / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D008858:Microscopy, Phase-Contrast; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D018417:Th1 Cells / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D007375:Interleukin-1; D053727:Interleukin-18 Receptor alpha Subunit; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D000097802:CD83 Antigen; C419393:CD1a antigen; C508572:IL18R1 protein, human; C508588:IL36B protein, human; C508589:IL36G protein, human