title: Development of a fast ELISA for quantifying polio D-antigen in in-process samples.
abstract: A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol was optimized by minimizing the total incubation time to 1 h, and by replacing the signal reagent 3,3',5,5'-tetramethylbenzidine by a chemiluminogenic signal reagent with a theoretical low intrinsic background and high dynamic range.
authors.affiliation: Unit Vaccinology, CIb, Centre for Infectious Disease Control, RIVM, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Rimko.ten.Have@RIVM.nl
authors.forename: R
authors.initials: R
authors.lastname: ten Have
authors.forename: Y E
authors.initials: YE
authors.lastname: Thomassen
authors.forename: M R J
authors.initials: MR
authors.lastname: Hamzink
authors.forename: W A M
authors.initials: WA
authors.lastname: Bakker
authors.forename: O E M
authors.initials: OE
authors.lastname: Nijst
authors.forename: G
authors.initials: G
authors.lastname: Kersten
authors.forename: G
authors.initials: G
authors.lastname: Zomer
mesh_terms: D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D017955:Poliovirus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral