title: The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism.
abstract: Several phenolic compounds as well as ascorbate can oxidise in certain cell culture media (especially Dulbecco's modified Eagle's medium (DMEM)) to generate hydrogen peroxide. Addition of oxaloacetate decreased the levels of H(2)O(2) detected and the oxaloacetate was depleted. Oxaloacetate was approximately as effective as pyruvate in decreasing H(2)O(2) levels and more effective than α-ketoglutarate. Our data raise important issues to consider when interpreting the behaviour and metabolism of cells in culture (which are both altered by the oxidative stress of cell culture) and their apparent response to addition of autooxidisable compounds such as ascorbate and epigallocatechin gallate.
authors.affiliation: Department of Biochemistry, National University of Singapore, Singapore.
authors.forename: Lee Hua
authors.initials: LH
authors.lastname: Long
authors.forename: Barry
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002477:Cells / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D062907:Oxaloacetic Acid / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D003470:Culture Media; D062907:Oxaloacetic Acid; D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D001205:Ascorbic Acid