title: Protective effects of total alkaloidal extract from Murraya koenigii leaves on experimentally induced dementia.
abstract: Dementia is a syndrome of gradual onset and continuous decline of higher cognitive functioning. It is a common disorder in older persons and has become more prevalent today. The fresh leaves of Murraya koenigii are often added to various dishes in Asian countries due to the delicious taste and flavor that they impart. These leaves have also been proven to have health benefits. In the present study, the effect of total alkaloidal extract from M. koenigii leaves (MKA) on cognitive functions and brain cholinesterase activity in mice were determined. In vitro β-secretase 1 (BACE1) inhibitory activity was also evaluated. The total alkaloidal extract was administered orally in three doses (10, 20 and 30 mg/kg) for 15 days to different groups of young and aged mice. Elevated plus maze and passive avoidance apparatus served as the exteroceptive behavioral models for testing memory. Diazepam-, scopolamine-, and ageing-induced amnesia served as the interoceptive behavioral models. MKA (20 and 30 mg/kg, p.o.) showed significant improvement in memory scores of young and aged mice. Furthermore, the same doses of MKA reversed the amnesia induced by scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). Interestingly, the brain cholinesterase activity was also reduced significantly by total alkaloidal extract of M. koenigii leaves. The IC50 value of MKA against BACE1 was 1.7 μg/mL. In conclusion, this study indicates MKA to be a useful remedy in the management of Alzheimer's disease and dementia.
authors.affiliation: Brain Research Laboratory, Faculty of Pharmacy, Puncak Alam Campus, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia. vasudevan@puncakalam.uitm.edu.my
authors.forename: Vasudevan
authors.initials: V
authors.lastname: Mani
authors.forename: Kalavathy
authors.initials: K
authors.lastname: Ramasamy
authors.forename: Aliya
authors.initials: A
authors.lastname: Ahmad
authors.forename: Milind
authors.initials: M
authors.lastname: Parle
authors.forename: Syed Adnan Ali
authors.initials: SA
authors.lastname: Shah
authors.forename: Abu Bakar Abdul
authors.initials: AB
authors.lastname: Majeed
mesh_terms: D000470:Alkaloids / Q000494:pharmacology / Q000627:therapeutic use*; D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors; D001921:Brain / Q000201:enzymology; D002802:Cholinesterases / Q000187:drug effects; D003704:Dementia / Q000188:drug therapy* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D032101:Murraya / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D002802:Cholinesterases; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509282:Bace1 protein, mouse