title: Circular IGFBP-3 level affected by the gene transfer of different growth hormone isoforms.
abstract: To evaluate whether through gene transfer, which growth hormone (GH) isoform can influence the circulation IGFBP-3 level, mice were injected three times with the human cytomegalovirus (CMV) mediated mammalian expression vector encoding different GH isoform gene. Then blood samples were drawn and the ELISA method was used to determine the circulation concentration of IGFBP-3. It was found that the 22 kDa GH gene could increase IGFBP-3 levels to 1.94 microg/ mL whereas 20 kDa GH, 17 kDa GH and 5 kDa GH genes did not play a role in the circulation expression of IGFBP-3.
authors.affiliation: Department of Biological Science and Engineering, School of Chemical and Biological Engineering, University of Science and Technology Beijing, Beijing, PR China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Li
authors.forename: Guangyu
authors.initials: G
authors.lastname: Chen
authors.forename: Haimin
authors.initials: H
authors.lastname: Jin
authors.forename: Tian
authors.initials: T
authors.lastname: Hua
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.forename: Linhui
authors.initials: L
authors.lastname: Cai
authors.forename: Jieyong
authors.initials: J
authors.lastname: Wang
authors.forename: Yiping
authors.initials: Y
authors.lastname: Xun
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D013006:Growth Hormone / Q000737:chemistry* / Q000235:genetics*; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D015183:Restriction Mapping
chemical_list: C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D013006:Growth Hormone