title: Influence of the Chungkookjang on histamine-induced wheal and flare skin response: a randomized, double-blind, placebo controlled trial.
abstract: BACKGROUND
  Allergic disease is a consequence of exposure to normally innocuous substances that elicit the activation of mast cells. Mast-cell-mediated allergic response is involved in many diseases such as anaphylaxis, urticaria, allergic rhinitis, asthma and allergic dermatitis. The development of food products for the prevention of allergic disease is an important subject in human health. The chungkookjang (CKJ) has been reported to exhibit antiallergic inflammatory activity. Therefore, the aim of the study is to examine the effects of the CKJ to reduce histamine-induced wheal and flare skin responses.
  
  
  METHODS
  A randomized, double-blind, placebo-controlled study in 60 healthy subjects will be carried out. Sixty volunteers (aged 20-80) who gave a written consent before entering the study will be randomized in two groups of thirty subjects each. The skin prick test with histamine solution of 10 mg/ml will be performed on the ventral forearm, 10 cm from the elbow. The subjects will be instructed to take 35 g per day of either the CKJ pills or a placebo pills for a period of 3 months. Diameters of wheal and flare will be assessing 15 minutes after performing the above-mentioned skin prick test. The primary outcome is change in wheal and flare responses. Secondary outcomes will be include change in serum histamine, immunoglobulin E, cytokines (interferon-gamma, interleukin-4, -10, and tumor necrosis factor-alpha), and eosinophil cationic protein.
  
  
  CONCLUSIONS
  This study will show the potential anti-inflammatory properties of the CKJ in their skin activity when histamine is the challenging agent as occurs in the clinical situation. And the present protocol will confirm the efficacy and safety of the CKJ for allergy symptoms, suggesting more basic knowledge to conduct further randomized controlled trials (RCT). If this study will be successfully performed, the CKJ will be an alternative dietary supplemental remedy for allergy patients.
  
  
  BACKGROUND
  NCT01402141.
authors.affiliation: Clinical Trial Center for Functional Foods of Chonbuk National University Hospital, Keumam-dong, Deokjin-gu, Jeonju City 561-712, Republic of Korea.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hee-Joo
authors.initials: HJ
authors.lastname: Kang
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ki-Chan
authors.initials: KC
authors.lastname: Ha
authors.forename: Hyang-Im
authors.initials: HI
authors.lastname: Back
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Eun-Ok
authors.initials: EO
authors.lastname: Park
authors.forename: Min-Gul
authors.initials: MG
authors.lastname: Kim
authors.forename: Seok-Kwon
authors.initials: SK
authors.lastname: Yun
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Chae
authors.forename: Back-Hwan
authors.initials: BH
authors.lastname: Cho
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D002985:Clinical Protocols; D004311:Double-Blind Method; D005260:Female; D006632:Histamine / Q000276:immunology*; D006634:Histamine H1 Antagonists / Q000627:therapeutic use*; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D007529:Isoflavones / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000187:drug effects / Q000276:immunology*; D012882:Skin Tests; D030262:Soybean Proteins / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D018926:Anti-Allergic Agents; D006634:Histamine H1 Antagonists; D007529:Isoflavones; D030262:Soybean Proteins; C525760:chungkookjang; D006632:Histamine