title: Detection of stapylococcal enterotoxin, methicillin-resistant and Panton-Valentine leukocidin genes in coagulase-negative staphylococci isolated from cows and ewes with subclinical mastitis.
abstract: Coagulase-negative staphylococci (CNS) are the most prevalent mastitis pathogens. However, virulence characteristics of CNS have not been well determined. The presence of genes for enterotoxins (sea-sej), toxic shock syndrome toxin-1 (tst), the exfoliative toxins (eta, etb), Panton-Valentine leukocidin (pvl) and mecA of CNS species isolated from cows and ewes with subclinical mastitis was investigated in this study. A total of 121 CNS (81 cows, 40 ewes) representing 18 different Staphylococci species were examined by PCR, and 38.1% (33 cows and 13 ewes) of CNS isolates had one or more se genes. The difference between percentages for SE toxin genes of CNS strains isolated from cows (40.7%) and ewes (32.5%) was not statistically significant (P > 0.05; χ(2) = 0.380). It was found that S. simulans isolates had the highest prevalent se genes. Furthermore, the most common SE gene types was seh-sej. In this study, none of the isolates harbored the toxic shock syndrome toxin gene (tsst) and the exfoliative toxin genes (eta, etb). Five cow (6.17%) and three ewe CNS (7.5%) isolates had mecA gene. Three cow (3.7%) and two ewe CNS (5.0%) isolates had pvl gene. In conclusion, the present study showed that CNS species isolated from cows and ewes could serve as potential reservoir of se, mecA, and pvl genes.
authors.affiliation: Faculty of Veterinary Medicine, Department of Microbiology, Kirikkale University, Kirikkale, Turkey. nilkarakaya@hotmail.com
authors.forename: Nilgün
authors.initials: N
authors.lastname: Unal
authors.forename: Oya Doğu
authors.initials: OD
authors.lastname: Cinar
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002417:Cattle; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D005260:Female; D007956:Leukocidins / Q000235:genetics*; D008414:Mastitis, Bovine / Q000382:microbiology*; D016106:Methicillin Resistance / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; C031818:enterotoxin B, staphylococcal