title: Tripchlorolide induces cell death in lung cancer cells by autophagy.
abstract: It has been demonstrated that triptolide inhibits the growth of several             types of cancer cells in vitro and prevents tumor growth in vivo by inducing apoptosis             and autophagy. Here we showed that Tripchlorolide (T4) significantly suppressed             the proliferation of A549 cells in a dose- and time-dependent manner. This suppressive             effect was diminished when cells were pretreated with 3-Methylamphetamine (3-MA).             After the cells were treated with T4, the LC3 II protein expression was significantly             increased, and autophagosomes were observed by TEM. However, almost no apoptosis             was observed in A549 treated with T4. These results suggest that T4 induces A549             cell death predominantly through the activation of the autophagy pathway instead             of the apoptosis pathway.
authors.affiliation: Department of Respiratory Medicine, Union Hospital, Fujian Medical University, Fuzhou, Fujian 350001, PR China.
authors.forename: Limin
authors.initials: L
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Li
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Chen
authors.forename: Tingyan
authors.initials: T
authors.lastname: Lin
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010616:Phenanthrenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004224:Diterpenes; D010616:Phenanthrenes; C068051:tripchlorolide