title: Cellulosibacter alkalithermophilus gen. nov., sp. nov., an anaerobic alkalithermophilic, cellulolytic-xylanolytic bacterium isolated from soil of a coconut garden.
abstract: An obligately anaerobic, cellulolytic-xylanolytic bacterium, designated strain A6(T), was isolated from soil of a coconut garden in the Bangkuntien district of Bangkok, Thailand. The strain was Gram-stain positive, catalase-negative, endospore-forming, motile and rod-shaped with a cell size of 0.2-0.3×2.0-3.0 µm. Optimal growth of strain A6(T) occurred at pH(55 °C) 9.5, 55 °C. Strain A6(T) fermented various carbohydrates, and the end products from the fermentation of cellobiose were acetate, ethanol, propionate and a small amount of butyrate. The major cellular fatty acids were iso-C(14:0) 3-OH, iso-C(15:0), iso-C(16:0) and C(16:0). The cell-wall peptidoglycan contained meso-diaminopimelic acid. No respiratory quinones were detected. The DNA G+C content was 30.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a new phyletic sublineage within the family Clostridiaceae, with <93.0% 16S rRNA gene sequence similarity to recognized species of this family. On the basis of phenotypic, genotypic and physiological evidence, strain A6(T) represents a novel species of a new genus, for which the name Cellulosibacter alkalithermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is A6(T) ( = TISTR 1915(T) = KCTC 5874(T)).
authors.affiliation: Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.
authors.forename: Amornrat
authors.initials: A
authors.lastname: Watthanalamloet
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
authors.forename: Chakrit
authors.initials: C
authors.lastname: Tachaapaikoon
authors.affiliation: Department of Medical Zoology, College of Medicine, Kyung Hee University, Dongdaemungu, Seoul 130-701, Republic of Korea.
authors.forename: Yun Sik
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Post-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
authors.affiliation: Post-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.affiliation: Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.
authors.forename: Khin Lay
authors.initials: KL
authors.lastname: Kyu
authors.affiliation: Enzyme Technology Laboratory, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D003059:Cocos; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013785:Thailand
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S