title: Continuous bio-catalytic conversion of sugar mixture to acetone-butanol-ethanol by immobilized Clostridium acetobutylicum DSM 792.
abstract: Continuous production of acetone, n-butanol, and ethanol (ABE) was carried out using immobilized cells of Clostridium acetobutylicum DSM 792 using glucose and sugar mixture as a substrate. Among various lignocellulosic materials screened as a support matrix, coconut fibers and wood pulp fibers were found to be promising in batch experiments. With a motive of promoting wood-based bio-refinery concept, wood pulp was used as a cell holding material. Glucose and sugar mixture (glucose, mannose, galactose, arabinose, and xylose) comparable to lignocellulose hydrolysate was used as a substrate for continuous production of ABE. We report the best solvent productivity among wild-type strains using column reactor. The maximum total solvent concentration of 14.32 g L(-1) was obtained at a dilution rate of 0.22 h(-1) with glucose as a substrate compared to 12.64 g L(-1) at 0.5 h(-1) dilution rate with sugar mixture. The maximum solvent productivity (13.66 g L(-1) h(-1)) was obtained at a dilution rate of 1.9 h(-1) with glucose as a substrate whereas solvent productivity (12.14 g L(-1) h(-1)) was obtained at a dilution rate of 1.5 h(-1) with sugar mixture. The immobilized column reactor with wood pulp can become an efficient technology to be integrated with existing pulp mills to convert them into wood-based bio-refineries.
authors.affiliation: Department of Biotechnology and Chemical Technology, Aalto University School of Chemical Engineering, Aalto, Finland.
authors.forename: Shrikant A
authors.initials: SA
authors.lastname: Survase
authors.forename: Adriaan
authors.initials: A
authors.lastname: van Heiningen
authors.forename: Tom
authors.initials: T
authors.lastname: Granström
mesh_terms: D000096:Acetone / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D000440:Butanols / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D009005:Monosaccharides / Q000378:metabolism*
chemical_list: D000440:Butanols; D009005:Monosaccharides; D000096:Acetone; D000431:Ethanol