title: An efficient analytical method for analysis of spirotetramat and its metabolite spirotetramat-enol by HPLC.
abstract: Spirotetramat is a new compound which belongs to the chemical class of ketoenols. As per the available literature analysis of spirotetramat and its metabolites spirotetramat-enol is carried out by high pressure liquid chromatograph with mass spectrometry (HPLC-MS/MS). In this study we have standardized a method where analysis of both the compounds is carried out by HPLC. The extraction and cleanup of spirotetramat and its metabolites spirotetramat-enol was carried out by QuEChERS method. The cleaned up residues were estimated by HPLC equipped with a photo diode array detector at a wavelength of 250 nm. The mobile phase used was acetonitrile: water at a proportion of 40:60. The limit of quantification (LOQ) of the method was 0.05 mg kg(-1) for both spirotetramat and its metabolite spirotetramat-enol. The recoveries of both the compounds at the LOQ level were in the range of 72.72%-86.76% from mango and 74.82% to 86.92% from cabbage.
authors.affiliation: Pesticide Residue Laboratory, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Karnataka, Bangalore 560089, India. Soudamini_mohapatra@rediffmail.com
authors.forename: Soudamini
authors.initials: S
authors.lastname: Mohapatra
authors.forename: M
authors.initials: M
authors.lastname: Deepa
authors.forename: G K
authors.initials: GK
authors.lastname: Jagadish
mesh_terms: D001372:Aza Compounds / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005638:Fruit / Q000737:chemistry; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry; D013141:Spiro Compounds / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D001372:Aza Compounds; D007306:Insecticides; D013141:Spiro Compounds; C570705:spirotetramat