title: Polymorphisms of vascular cell adhesion molecule1 (VCAM1) in polycystic ovary syndrome determined by quantitative real-time polymerase chain reaction and melting curve analysis.
abstract: OBJECTIVE
  Polycystic ovary syndrome (PCOS) is a common endocrinopathy associated with increased risk of obesity, insulin resistance (IR) and type 2 diabetes mellitus. Low-grade chronic inflammation and imbalance between pro- and anti-inflammatory cytokines has been proposed to play a role in the pathogenesis. Vascular cell adhesion molecule1 (VCAM1) is among the parameters reflecting low-grade chronic inflammation whose expression is increased by pro-inflammatory cytokines. This study examined the possible association of T-1591C and T-833C single nucleotide polymorphisms (SNPs) of VCAM1 gene with the occurrence and the clinical/biochemical characteristics of PCOS.
  
  
  METHODS
  We analyzed genotype and allele distributions of the above-mentioned SNPs in DNA from peripheral blood leukocytes of 169 patients with PCOS and 179 healthy women, by a real-time polymerase chain reaction (PCR) method combined with melting curve analysis using fluorescence-labeled hybridization probes.
  
  
  RESULTS
  No significant associations between PCOS and the variant alleles of VCAM1-1591 (OR: 1.09, 95% CI=0.74-1.58) and -833 (OR: 1.42, 95% CI=0.59-3.43) were observed. None of the studied polymorphisms was found to affect IR indices and sVCAM levels significantly. However, PCOS women heterozygous for VCAM1-1591 polymorphism (CT) had significant increased triglyceride and decreased HDL-C in comparison with wild homozygous (TT) ones.
  
  
  CONCLUSIONS
  Although there is no association between -1591 and -833 polymorphisms of VCAM1 gene and susceptibility to PCOS, higher triglyceride and lower HDL-C in VCAM1-1591 CT genotype suspect that heterozygous patients are prone to increased risk for atherosclerosis and cardiovascular disease. In addition, bearing in mind that PCOS is a consequence of interaction between various genetic and environmental factors, the association between heterozygocity of VCAM1-1591 polymorphism and some lipid parameters may depend on the impact of other known or unknown polymorphisms, being in linkage disequilibrium with this locus of VCAM1 gene.
authors.affiliation: Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Çapa 34093, Istanbul, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Kanmaz-Özer
authors.forename: Pervin
authors.initials: P
authors.lastname: Vural
authors.forename: Semra
authors.initials: S
authors.lastname: Doğru-Abbasoğlu
authors.forename: Ali
authors.initials: A
authors.lastname: Gedikbaşı
authors.forename: Esra
authors.initials: E
authors.lastname: Çil
authors.forename: Berrin
authors.initials: B
authors.lastname: Karadağ
authors.forename: Müjdat
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D002648:Child; D008076:Cholesterol, HDL / Q000097:blood; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006579:Heterozygote; D006358:Hot Temperature; D006801:Humans; D015228:Hypertriglyceridemia / Q000235:genetics; D007333:Insulin Resistance; D015810:Linkage Disequilibrium; D009691:Nucleic Acid Denaturation; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014421:Turkey; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry / Q000235:genetics*; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D019010:Vascular Cell Adhesion Molecule-1