title: Recombinant Whirly1 translocates from transplastomic chloroplasts to the nucleus.
abstract: Whirly1 was shown to be dually located in chloroplasts and nucleus of the same cell. To investigate whether the protein translocates from chloroplasts to the nucleus, we inserted a construct encoding an HA-tagged Whirly1 into the plastid genome of tobacco. Although the tagged protein was synthesized in plastids, it was detected in nuclei. Dual location of the protein was confirmed by immunocytological analyses. These results indicate that the plastidial Whirly1 is translocated from the plastid to the nucleus where it affects expression of target genes such as PR1. Our results support a role of Whirly1 in plastid-nucleus communication.
authors.affiliation: Institute of Botany, Christian-Albrechts-University of Kiel, Olshausenstraße 40, 24098 Kiel, Germany.
authors.forename: Rena
authors.initials: R
authors.lastname: Isemer
authors.forename: Maria
authors.initials: M
authors.lastname: Mulisch
authors.forename: Anke
authors.initials: A
authors.lastname: Schäfer
authors.forename: Stefan
authors.initials: S
authors.lastname: Kirchner
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Koop
authors.forename: Karin
authors.initials: K
authors.lastname: Krupinska
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002736:Chloroplasts / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D018087:Plastids / Q000235:genetics; D021381:Protein Transport; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; C503333:Whirly1 protein, Arabidopsis