title: [Diagnostic efficiency of Indirect Hemagglutination Assay Kit for antibody detection of schistosomiasis japonica].
abstract: OBJECTIVE
  To evaluate the clinical diagnostic efficiency of Indirect Hemagglutination Assay Kit (IHA kit) for antibody detection of schistosomiasis japonica.
  
  
  METHODS
  Schistosomiasis examinations by parasitological method were conducted in the schistosomiasis endemic areas in Jiangxi, Anhui and Hubei provinces, respectively. A total of 371 serum samples of schistosomiasis patients and 761 serum samples of non-patients without history of schistosome infection and infested water contact were collected. All the sera were detected by using IHA Kit. The diagnostic efficiency indicators of the kit, including sensitivity, specificity, positive predicted value, negative predicted value etc. were calculated.
  
  
  RESULTS
  The sensitivity and specificity of the kit were 95.1% (95% confidence interval was between 92.9% and 97.3%) and 97.8% (95% confidence interval was between 96.8% and 98.8%), respectively; the positive and negative predicted values were 95.4% and 97.6%, respectively. No statistically significant difference was found in the prevalence of IHA positive detecting by the kit among the groups with different EPG levels (< or = 40, 41-100, > 100). Also, no significant difference was observed in sensitivity and specificity among the different age groups.
  
  
  CONCLUSIONS
  The IHA kit shows a relative high efficiency for diagnosis and can be used for mass screening of schistosomiasis in field sites.
authors.affiliation: Jiangxi Institute of Parasitic Diseases, Nanchang 330046, China.
authors.forename: Nian-Gao
authors.initials: NG
authors.lastname: Chen
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lin
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Liu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zeng
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Liu
authors.forename: Mei-Jiao
authors.initials: MJ
authors.lastname: Huang
authors.forename: Hong-Gen
authors.initials: HG
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002648:Child; D003955:Diagnostic Tests, Routine / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012549:Schistosoma japonicum / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D011933:Reagent Kits, Diagnostic