title: A method for rapid regulation of protein expression in Trypanosoma cruzi.
abstract: Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Jack and Pearl Resnick Campus, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Yan Fen
authors.initials: YF
authors.lastname: Ma
authors.forename: Louis M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Huan
authors.initials: H
authors.lastname: Huang
mesh_terms: D005786:Gene Expression Regulation; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D015800:Protozoan Proteins / Q000096:biosynthesis; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins