title: Protective effects of Eucommia lignans against hypertensive renal injury by inhibiting expression of aldose reductase.
abstract: OBJECTIVE
  To investigate the protective effects and the underlying mechanism of Eucommia lignans against hypertensive renal injury.
  
  
  METHODS
  Ten-week-old Wistar Kyoto rats and age matched spontaneously hypertension rats were used in the study. The SHR were randomly divided into 4 groups (n=7 for each group) and received different treatment for 16 weeks, which including saline, Captopril, Epalrestat and Eucommia lignans, respectively. System blood pressures of the rats were monitored once every 4 weeks. N-Acetyl-β-D-glucosaminidase (NAG) activity and the ratio of albumin and urinary creatinine were chosen as the indices of kidney function. Then the structure and renal collagen type III expression of glomerular basement membrane were observed by microscopy and the renal aldose reductase (AR) expression was measured by immunohistochemistry. In vitro, the proliferation of mesangial cells induced by AngII was assayed by MTT, and the mRNA expression of AR was measured by RT-real-time PCR.
  
  
  RESULTS
  The renal function, evaluated by NAG enzyme activity and the ratio of albumin to urinary creatinine, was significantly ameliorated by Eucommia lignans treatment. Meanwhile, Eucommia lignans decreased both the protein (P<0.05) and the mRNA expressed lever of AR (P<0.05). Eucommia lignans also decreased the high expression of collagen type III in SHR (P<0.05) and inhibited the proliferation of renal mesangial cells induced by AngII (P<0.05).
  
  
  CONCLUSIONS
  Eucommia lignans have protective effects against hypertensive renal injury, and the protective effects may be partly due to inhibition of aldose reductase.
authors.affiliation: Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Kai
authors.initials: K
authors.lastname: Hu
authors.forename: Juan
authors.initials: J
authors.lastname: Gu
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Deng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xian
authors.initials: X
authors.lastname: Jing
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
authors.forename: Qi-Fa
authors.initials: QF
authors.lastname: Ye
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Ouyang
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000419:Albuminuria / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000652:urine; D001794:Blood Pressure / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024061:Collagen Type III / Q000378:metabolism; D003404:Creatinine / Q000652:urine; D004195:Disease Models, Animal; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D031283:Eucommiaceae / Q000737:chemistry; D050533:Glomerular Basement Membrane / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D006973:Hypertension / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D007674:Kidney Diseases / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013997:Time Factors
chemical_list: D000959:Antihypertensive Agents; D015415:Biomarkers; D024061:Collagen Type III; D004791:Enzyme Inhibitors; D017705:Lignans; D010936:Plant Extracts; D012333:RNA, Messenger; D003404:Creatinine; D000449:Aldehyde Reductase; D000118:Acetylglucosaminidase