title: BAG2 is a target of the c-Myc gene and is involved in cellular senescence via the p21(CIP1) pathway.
abstract: Suppression of c-Myc is likely to induce cellular senescence in many tumors with unclear mechanisms. A proteomics survey indicated that high levels of BCL2-associated athanogene 2 (BAG2) were found in response to c-Myc repression in TRE293 cells. This observation led to the investigation into the role of BAG2 in c-Myc-induced senescence. The association of the c-Myc/SP1 complex with the BAG2 promoter verified the role of c-Myc/SP1 in regulating BAG2 transcription. Furthermore, high levels of BAG2 were found to induce p21(CIP1)-dependent senescence and subsequent carcinogenetic arrest, suggesting its possible role as an indirect activator of the p21(CIP1) pathway.
authors.affiliation: Laboratory of Synthetic Biology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Lou
authors.forename: Shangbin
authors.initials: S
authors.lastname: Yang
authors.forename: Shun
authors.initials: S
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shan
authors.forename: Siyuan
authors.initials: S
authors.lastname: Su
authors.forename: Qimin
authors.initials: Q
authors.lastname: Zhan
authors.forename: Ningzhi
authors.initials: N
authors.lastname: Xu
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D016922:Cellular Senescence; D047369:Chromatin Immunoprecipitation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: C492980:BAG2 protein, human; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018832:Molecular Chaperones; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016329:Sp1 Transcription Factor; D008156:Luciferases