title: Increase in pectin deposition by overexpression of an ERF gene in cultured cells of Arabidopsis thaliana.
abstract: Ethylene-responsive transcription factor (ERF) family genes, which are involved in regulation of metabolic pathways and/or are useful for metabolic engineering, were investigated in the cultured cells of Arabidopsis thaliana. The pectin content in the gelatinous precipitates after the ethanol precipitation of extracts derived from calli of a transgenic cell line, A17, overexpressing an ERF gene (At1g44830), increased in comparison with the control. Expression of genes involved in pectin biosynthesis was up-regulated in the A17 calli. Overexpression of the ERF gene coordinately activates the pectin biosynthetic pathway genes and increases the content of pectin. These results therefore will be useful as a genetic resource for engineering pectin biosynthesis in plants.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. tonakano@affrc.go.jp
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Nakano
authors.forename: Yuki
authors.initials: Y
authors.lastname: Naito
authors.forename: Koich
authors.initials: K
authors.lastname: Kakegawa
authors.forename: Namie
authors.initials: N
authors.lastname: Ohtsuki
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Tsujimoto-Inui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shinshi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D010368:Pectins / Q000096:biosynthesis*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; C093680:ethylene-responsive element binding protein; D010368:Pectins