title: Use of phage display for the identification of molecular sensors specific for activated Rho.
abstract: We describe a phage display approach to select active Rho-specific scFv sensors. This in vitro technique allows preserving the antigen conformation stability all along the selection process. We used the GTP locked RhoBQ63L mutant as antigen against the Griffin.1 library composed of a human synthetic V(H) + V(L) scFv cloned in the pHEN2 phagemid vector. The method described here has permitted to identify an scFv that discriminates between the activated and the inactivated form of the Rho subfamily.
authors.affiliation: INSERM UMR 1037, Cancer Research Centre of Toulouse, Claudius Regaud Cancer Institute, University of Toulouse, Toulouse, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Chinestra
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lajoie-Mazenc
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Faye
authors.forename: Gilles
authors.initials: G
authors.lastname: Favre
mesh_terms: D000918:Antibody Specificity; D015374:Biosensing Techniques / Q000379:methods*; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000302:isolation & purification / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; D020741:rho GTP-Binding Proteins