title: [Effect of shRNA Survivin-mediated inhibition of proliferation in A549 cells].
abstract: OBJECTIVE
  Survivin is a member of the inhibitor of apoptosis family of proteins. The Survivin protein is highly expressed in most human tumors, but it is completely absent in terminally differentiated cells. Consequently, Survivin is an ideal target for cancer therapy because cancer cells are targeted and normal cells are left alone. The aim of this study is to construct a lentivirus-shRNA vector, and to disrupt the expression of Survivin in A549 cells. The effect of sh-RNA Survivin on A549 cells was analyzed.
  
  
  METHODS
  Target DNA sequences of Survivin shRNA were designed to obtain recombinant plasmids. After the plasmids were transfected into 293T cells, the virus was collected. Hela cells were used to detect the virus titer. Survivin mRNA and protein expression in the infected A549 cells were detected via reverse transcription polymerase chain reaction and Western blot analysis. The proliferation of A549 cells were detected via 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide and flow cytometry assays.
  
  
  RESULTS
  The recombinants were successfully constructed, and Survivin expression was inhibited. The cells were blocked at the G2/M phase.
  
  
  CONCLUSIONS
  Recombinant lentivirus with shRNA targeting Survivin was successfully constructed. The lentivirus can down-regulate Survivin expression in A549 cells as well as inhibit proliferation, and is hence a potential gene therapy for lung cancer.
authors.affiliation: Tuberculosis Research Institute, the 309th Hospital of Chinese PLA, Beijing 100091, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Wu
authors.forename: Jinhe
authors.initials: J
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dongdong
authors.initials: D
authors.lastname: Jia
authors.forename: Jianqin
authors.initials: J
authors.lastname: Liang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin