title: Chromatographic approaches for determination of low-molecular mass aldehydes in bio-oil.
abstract: HPLC-UV and GC/MS determination of aldehydes in bio-oil were evaluated. HPLC-UV preceded by derivatization with 2,4-dinitrophenylhydrazine allows separation and detection of bio-oil aldehydes, but the derivatization affected the bio-oil stability reducing their quantitative applicability. GC/MS determination of aldehydes was reached by derivatization with o-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride. Two approaches for this reaction were evaluated. The first: "in solution derivatization and head space extraction" and the second: "on fiber derivatization SPME", the latter through an automatic procedure. Both sample treatments allows the quantification of most important aliphatic aldehydes in bio-oil, being the SPME approach more efficient. The aldehyde concentrations in bio-oil were ~2% formaldehyde, ~!0.1% acetaldehyde and ~0.05% propionaldehyde.
authors.affiliation: Departamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tessini
authors.forename: Niels
authors.initials: N
authors.lastname: Müller
authors.forename: Claudia
authors.initials: C
authors.lastname: Mardones
authors.forename: Dietrich
authors.initials: D
authors.lastname: Meier
authors.forename: Alex
authors.initials: A
authors.lastname: Berg
authors.forename: Dietrich
authors.initials: D
authors.lastname: von Baer
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D000704:Analysis of Variance; D056804:Biofuels / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006898:Hydroxylamines / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods; D005466:Fluorocarbons
chemical_list: D000447:Aldehydes; D056804:Biofuels; D006898:Hydroxylamines; D011108:Polymers; C010277:Florox Reagent; D005466:Fluorocarbons