title: Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus.
abstract: Glucokinase/hexokinase catalyzes the phosphorylation of glucose to glucose 6-phosphate, which is the first step of glycolysis. The open reading frame TTHA0299 of the extreme thermophile Thermus thermophilus encodes a putative glucokinase/hexokinase which contains the consensus sequence for proteins from the repressors, open reading frames and sugar kinases family. In this study, the glucokinase/hexokinase from T. thermophilus was purified and crystallized using polyethylene glycol 8000 as a precipitant. Diffraction data were collected and processed to 2.02 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 70.93, b = 138.14, c = 75.16 Å, β = 95.41°.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kashima
authors.forename: Shouhei
authors.initials: S
authors.lastname: Mine
authors.forename: Takashi
authors.initials: T
authors.lastname: Oku
authors.forename: Koichi
authors.initials: K
authors.lastname: Uegaki
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005941:Glucokinase / Q000737:chemistry*; D006593:Hexokinase / Q000737:chemistry*; D016963:Thermus thermophilus / Q000201:enzymology*
chemical_list: D006593:Hexokinase; D005941:Glucokinase