title: Electron transfer mechanism of cytochrome c at the oil/water interface as a biomembrane model.
abstract: The electron transfer (ET) between cytochrome c (Cyt c) in water (W) and 1,1'-dimethylferrocene (DiMFc) in 1,2-dichloroethane (DCE) was studied. The cyclic voltammograms obtained for the interfacial ET under various conditions could be well reproduced by digital simulation based on the ion-transfer (IT) mechanism, in which the ET process occurs not at the DCE/W interface but in the W phase nearest the interface. In this mechanism, the current signal is due to the IT of DiMFc(+) as the reaction product. On the other hand, the measurement of the double-layer capacity showed that Cyt c is adsorbed at the DCE/W interface. However, the contribution from the adsorbed proteins to the overall ET is considered to be small because of the thicker reaction layer in the IT mechanism. These findings would offer a useful suggestion for the behaviors of Cyt c in vivo.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kobe University, Nada, Kobe, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Imai
authors.forename: Takayasu
authors.initials: T
authors.lastname: Sugihara
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Osakai
mesh_terms: D045304:Cytochromes c / Q000737:chemistry*; D055664:Electrochemical Techniques; D004579:Electron Transport; D005025:Ethylene Dichlorides / Q000737:chemistry; D008958:Models, Molecular; D009821:Oils / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D005025:Ethylene Dichlorides; D009821:Oils; D014867:Water; C024565:ethylene dichloride; D045304:Cytochromes c