title: Oceanitalea nanhaiensis gen. nov., sp. nov., an actinobacterium isolated from seawater.
abstract: A Gram-positive, motile, short-rod-shaped bacterium, designated strain JLT1488(T), was isolated from the South China Sea and investigated in a taxonomic study using a polyphasic approach. The peptidoglycan type determined for strain JLT1488(T) was A4α with lysine as the diagnostic cell-wall diamino acid and an interpeptide bridge of L-Lys-L-Glu. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unknown glycolipid and an unknown phospholipid. The only detected menaquinone was MK-8(H(4)), and the major fatty acids were summed feature 8 (C(18 : 1)ω7c/C(18 : 1)ω6c) , C(16 : 0) and summed feature 3 (C(16 : 1)ω7c/C(16 : 1)ω6c); significant amounts of C(12 : 0) 3-OH, C(10 : 0) and C(19 : 0) cyclo ω8c were also present. The G+C content of the genomic DNA was 62.3 mol%. Comparison of the 16S rRNA gene sequence of strain JLT1488(T) with those of related type strains demonstrated that it represented a novel lineage within the family Bogoriellaceae, suborder Micrococcineae, being closely related to species of the genera Georgenia, Bogoriella and Cellulomonas (94.6-96.8 % sequence similarity). These results demonstrate that strain JLT1488(T) is a member of a new genus, for which the name Oceanitalea nanhaiensis gen. nov., sp. nov. is proposed. The type strain of the type species is JLT1488(T) ( = JCM 17755(T) = CGMCC 1.10826(T)).
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yingnan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Qipei
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Keshao
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yongle
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, 450008, PR China.|State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S