title: IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation.
abstract: The mouse is a suitable experimental model to study the biology of mesenchymal stem cells (MSCs), as well as to be used in biocompatibility studies and tissue engineering models. However, the isolation and purification of murine MSCs is far more challenging than their counterparts from other species. In this study, we isolated, expanded and characterized mouse MSCs from bone marrow (BM-MSCs). Additionally, we analyzed the effects of two regulatory molecules, interleukin 17 (IL-17) and basic fibroblast growth factor (bFGF), on BM-MSCs growth and elucidated the signaling pathways involved. The results revealed that IL-17 increased the frequency of colony-forming units fibroblast (CFU-F) as well as the BM-MSCs proliferation in a dose-dependent manner, while bFGF supplementation had no significant effect on CFU-F frequency but induced an increase in cell proliferation. Their combined usage did not produce additive effects on BM-MSCs proliferation and even induced reduction in the number of CFU-F. Also, the involvement of both p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs) signaling in proliferative activity of IL-17 and bFGF on murine BM-MSCs and, moreover, the increased co-activation of a common signaling molecule, p38 MAPK, were demonstrated. Together, the data presented highlighted the role of IL-17 and bFGF in murine BM-MSCs proliferation and pointed to the complexity and specificity of the signaling networks leading to MSCs proliferation in response to different regulatory molecules.
authors.affiliation: Institute for Medical Research, University of Belgrade, Belgrade, Serbia. slavko@imi.bg.ac.rs
authors.forename: Slavko
authors.initials: S
authors.lastname: Mojsilović
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Krstić
authors.forename: Vesna
authors.initials: V
authors.lastname: Ilić
authors.forename: Ivana
authors.initials: I
authors.lastname: Okić-Đorđević
authors.forename: Jelena
authors.initials: J
authors.lastname: Kocić
authors.forename: Drenka
authors.initials: D
authors.lastname: Trivanović
authors.forename: Juan Francisko
authors.initials: JF
authors.lastname: Santibañez
authors.forename: Gordana
authors.initials: G
authors.lastname: Jovčić
authors.forename: Diana
authors.initials: D
authors.lastname: Bugarski
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D020381:Interleukin-17 / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction
chemical_list: D020381:Interleukin-17; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors