title: DNA binding protein A expression and methylation status in hepatocellular             carcinoma and the adjacent tissue.
abstract: We investigated the expression and promoter methylation of dbpA in human             hepatocellular carcinoma (HCC) and examined their correlation with clinicopathological             features. In 96 paired samples of HCC and adjacent non-tumorous liver, and 10             normal liver specimens, dbpA mRNA was quantified by real-time RT-PCR, and promoter             methylation was examined by methylation-specific polymerase chain reaction and             bisulfite sequencing. The results showed that dbpA mRNA expression levels were             higher in HCC compared to corresponding non-tumor tissues (P<0.01) and higher             in non-virus-associated HCC compared to virus-associated cases (P<0.01). dbpA             promoter was methylated in 37.7% of HCC samples and the promoter methylation was             significantly correlated with the low expression of dbpA in non-virus-associated             HCC (P<0.01), but not in virus-associated HCC. Surprisingly, poor prognosis             was more significantly associated with high dbpA expression in non-tumorous liver             (P=0.018) but not with that in HCC. Non-tumorous tissues consist of chronic hepatitis             or liver cirrhosis, and these conditions are the background of hepatocarcinogenesis,             defined as the hypercarcinogenic state. Our results suggest that the high expression             of dbpA in the hypercarcinogenic state is an indicator of poor prognosis.
authors.affiliation: Department of Hepato-Biliary-Pancreatic Surgery, Tokyo Medical and Dental University, Tokyo, Japan. mahmut@bioinfo.tmd.ac.jp
authors.forename: Mahmut
authors.initials: M
authors.lastname: Yasen
authors.forename: Gulanbar
authors.initials: G
authors.lastname: Obulhasim
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kajino
authors.forename: Kaoru
authors.initials: K
authors.lastname: Mogushi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizushima
authors.forename: Shinji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Okio
authors.initials: O
authors.lastname: Hino
authors.forename: Shigeki
authors.initials: S
authors.lastname: Arii
mesh_terms: D000368:Aged; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000235:genetics / Q000473:pathology; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011379:Prognosis; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; C094867:YBX3 protein, human