title: Laplace field microscopy for label-free imaging of dynamic biological structures.
abstract: We present Laplace field microscopy as a method for generating intrinsic contrast of transparent specimens. This technique uses a spatial light modulator to perform the Laplacian of the field in the Fourier plane of a microscope image. The resulting image incorporates phase information and thus renders high contrast images from phase objects. We demonstrate the potential of the method by imaging index-matched beads, unlabeled tissue slices, and dynamic live cells.
authors.affiliation: Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Taewoo
authors.initials: T
authors.lastname: Kim
authors.forename: Gabriel
authors.initials: G
authors.lastname: Popescu
mesh_terms: D004867:Equipment Design; D005583:Fourier Analysis; D006367:HeLa Cells; D006801:Humans; D008857:Microscopy, Interference / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D055096:Optical Devices; D055620:Optical Phenomena