title: Involvement of F-actin in chaperonin-containing t-complex 1 beta regulating mouse mesangial cell functions in a glucose-induction cell model.
abstract: The aim of this study is to investigate the role of chaperonin-containing t-complex polypeptide 1 beta (CCT2) in the regulation of mouse mesangial cell (mMC) contraction, proliferation, and migration with filamentous/globular-(F/G-) actin ratio under high glucose induction. A low CCT2 mMC model induced by treatment of small interference RNA was established. Groups with and without low CCT2 induction examined in normal and high (H) glucose conditions revealed the following major results: (1) low CCT2 or H glucose showed the ability to attenuate F/G-actin ratio; (2) groups with low F/G-actin ratio all showed less cell contraction; (3) suppression of CCT2 may reduce the proliferation and migration which were originally induced by H glucose. In conclusion, CCT2 can be used as a specific regulator for mMC contraction, proliferation, and migration affected by glucose, which mechanism may involve the alteration of F-actin, particularly for cell contraction.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 325, Section 2, Cheng-Kung Road, Neihu 114, Taipei, Taiwan.
authors.forename: Jin-Shuen
authors.initials: JS
authors.lastname: Chen
authors.forename: Li-Chien
authors.initials: LC
authors.lastname: Chang
authors.forename: Chia-Chao
authors.initials: CC
authors.lastname: Wu
authors.forename: Lai-King
authors.initials: LK
authors.lastname: Yeung
authors.forename: Yuh-Feng
authors.initials: YF
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D056404:Chaperonin Containing TCP-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008954:Models, Biological; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D000199:Actins; D034741:RNA, Small Interfering; C533315:Tcp1 protein, mouse; D056404:Chaperonin Containing TCP-1; D005947:Glucose