title: Rapid, non-targeted discovery of biochemical transformation and biomarker candidates in oncovirus-infected cell lines using LAESI mass spectrometry.
abstract: Finding insights into how viruses hijack metabolic processes and biomarkers for viral diseases often require hypotheses about target compounds and/or labelling techniques. Here we present a method based on laser ablation electrospray ionization mass spectrometry to rapidly identify potential protein and metabolite biomarkers of oncovirus infection in B lymphocytes.
authors.affiliation: Department of Chemistry, W. M. Keck Institute of Proteomics Technology and Applications, The George Washington University, Washington DC, USA.
authors.forename: Bindesh
authors.initials: B
authors.lastname: Shrestha
authors.forename: Prabhakar
authors.initials: P
authors.lastname: Sripadi
authors.forename: Callee M
authors.initials: CM
authors.lastname: Walsh
authors.forename: Trust T
authors.initials: TT
authors.lastname: Razunguzwa
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Powell
authors.forename: Kylene
authors.initials: K
authors.lastname: Kehn-Hall
authors.forename: Fatah
authors.initials: F
authors.lastname: Kashanchi
authors.forename: Akos
authors.initials: A
authors.lastname: Vertes
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D019288:Herpesvirus 8, Human; D006801:Humans; D007834:Lasers; D010713:Phosphatidylcholines / Q000378:metabolism*; D012192:Retroviridae Infections / Q000175:diagnosis / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013947:Thymosin / Q000378:metabolism*; D000099201:Thymosin beta(4)
chemical_list: D015415:Biomarkers; D010713:Phosphatidylcholines; D013947:Thymosin; D000099201:Thymosin beta(4)