title: Evaluation of an IgY-based immunomagnetic enzyme-linked immunosorbent assay system for detection of circulating Schistosoma japonicum antigen in serum samples from patients in China.
abstract: We have developed a novel egg yolk antibody (IgY)-coated magnetic beads antigen-capture immunoassay for detection of a circulating antigen of Schistosoma japonicum in serum samples of patients in schistosomiasis-endemic areas of China. This IgY-based immunomagnetic bead enzyme-linked immunosorbent assay (IgY-IMB-ELISA) uses polyclonal IgY-coated magnetic beads as a capture antibody, and a monoclonal IgG as a detection antibody. The sensitivity of the magnetic immunoassay was 100% (40 of 40) in cases of acute infection and 91.5% (107 of 117) in chronic cases of schistosomiasis, and no positive reaction was found in 0 of 49 healthy persons. Cross-reactivity was 3.3% (1 of 33) with clonorchiasis and 0% (0 of 20) with paragonimiasis. There was a significant correlation between ELISA absorbance value and egg count (eggs per gram feces) and a correlation coefficient of 0.88 in a small sample of 14 patients. The results demonstrated that the IgY-IMB-ELISA is a sensitive and specific assay for detection of human schistosomiasis japonica.
authors.affiliation: Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China. leijiahui@hotmail.com
authors.forename: Jia-hui
authors.initials: JH
authors.lastname: Lei
authors.forename: Bing-tao
authors.initials: BT
authors.lastname: Su
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Ji-long
authors.initials: JL
authors.lastname: Shen
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Guan
authors.forename: Zhen-qing
authors.initials: ZQ
authors.lastname: Feng
authors.forename: Yong-long
authors.initials: YL
authors.lastname: Li
authors.forename: Ming-xing
authors.initials: MX
authors.lastname: Xu
authors.forename: Wen-qi
authors.initials: WQ
authors.lastname: Liu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D018189:Immunomagnetic Separation / Q000379:methods*; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth; C016512:IgY; D007136:Immunoglobulins