title: The influence of osteoblast differentiation stage on bone formation in autogenously implanted cell-based poly(lactide-co-glycolide) and calcium phosphate constructs.
abstract: We tested the hypothesis that the osteoblast differentiation status of bone marrow stem cells (BMSCs) combined with a three-dimensional (3D) structure modulates bone formation when autogenously implanted. Rat BMSCs were aspirated, expanded, and seeded into a 3D composite of poly(lactide-co-glycolide) and calcium phosphate (PLGA/CaP) to produce a hybrid biomaterial. Calvarial defects were implanted with (1) scaffold without cells (SC/NC), (2) scaffold and BMSCs (SC+BMSC), (3) scaffold and osteoblasts differentiated for 7 days (SC+OB7), and (4) for 14 days (SC+OB14). After 4 weeks, there was more bone formation in groups combining scaffold and cells, SC+BMSC and SC+OB7. A nonsignificant higher amount of bone formation was observed on SC+OB14 compared with SC/NC. Additionally, more blood vessels were counted within all hybrid biomaterials, without differences among them, than into SC/NC. These findings provide evidences that the cell differentiation status affects in vivo bone formation in autogenously implanted cell-based constructs. Undifferentiated BMSCs or osteoblasts in early stage of differentiation combined with PLGA/CaP scaffold favored bone formation compared with plain scaffold and that one associated with more mature osteoblasts.
authors.affiliation: Cell Culture Laboratory, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
authors.forename: Marcio M
authors.initials: MM
authors.lastname: Beloti
authors.forename: Luciana G
authors.initials: LG
authors.lastname: Sicchieri
authors.forename: Paulo T
authors.initials: PT
authors.lastname: de Oliveira
authors.forename: Adalberto Luiz
authors.initials: AL
authors.lastname: Rosa
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid