title: Clinical trial of allogeneic cultured dermal substitutes for intractable skin ulcers.
abstract: The effect of allogeneic cultured dermal substitute (CDS) on wound healing was evaluated in 9 intractable skin ulcers in 5 patients who had failed to improve despite conventional topical treatment with basic fibroblast growth factor (bFGF) for more than 2 months. In general, the topical application of bFGF is effective in facilitating wound healing. However, skin regeneration was very slow in the present 9 cases. In this study, to improve the condition of these wounds, allogeneic CDS was applied once a week for 2 months. The wound healing process was evaluated, focusing on the reduction ratio of wound size through the granulation tissue formation associated with epithelialization. In all 9 cases, the wound size was successfully decreased after the application of CDS, and ulcers were completely resurfaced in 2 cases. In all cases, except the 2 cases showing complete wound closure, the mean wound size decreased to 33.3% of the original size, i.e., a mean reduction ratio of 33.3%. The present results indicate that allogeneic CDS can promote wound healing of intractable skin ulcers that fail to improve despite treatment with bFGF.
authors.affiliation: Department of Plastic and Aesthetic Surgery, Yokohama Minamikyousai Hospital, 1-21-1 Mutsuurahigashi, Kanazawa-ku, Yokohama, Kanagawa, 236-0037, Japan. ymprs@minamikyousai.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamada
authors.forename: Eiju
authors.initials: E
authors.lastname: Uchinuma
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kuroyanagi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use; D006801:Humans; D016038:Skin Transplantation / Q000379:methods*; D012883:Skin Ulcer / Q000601:surgery*; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous / Q000379:methods*; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*
chemical_list: D016222:Fibroblast Growth Factor 2