title: Isolation and purification of recombinant proteins, antibodies and plasmid DNA with hydroxyapatite chromatography.
abstract: Hydroxyapatite and related stationary phases increasingly play a role in the downstream processing of high-value biological materials, such as recombinant proteins, therapeutic antibodies and pharmaceutical-grade plasmid DNA. Chromatographic hydroxyapatite is an inorganic, ceramic material identical in composition, if not in structure, to calcium phosphate found in human bones and teeth. The interaction of hydroxyapatite with biomacromolecules is complex and highly dynamic, which can make predicting performance difficult, but also allows the design of very selective isolation processes. This review discusses the currently commercially available chromatographic materials, different retention mechanisms supported by these materials and differential exploitation for the design of highly specific isolation procedures. The state of the art of antibody purification by hydroxy- and fluoroapatite is reviewed together with tested routines for method development and implementation. Finally, the isolation of plasmid DNA is discussed, since the purification of DNA therapeutics at a sufficiently large scale is an emerging need in bioprocess development and perhaps the area in bioseparation where apatite chromatography can make its most important contribution to date.
authors.affiliation: University of Bayreuth, 95440 Bayreuth, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Hilbrig
authors.forename: Ruth
authors.initials: R
authors.lastname: Freitag
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D004247:DNA; D017886:Durapatite