title: Evaluation of the cytotoxicity of cigarette smoke condensate by a cellular impedance biosensor.
abstract: In this study, a cytotoxicity assay was developed for profiling the cytotoxicity of cigarette smoke condensates (CSCs) base on a cellular impedance biosensor (CIB). Compared with the traditional in vitro cytotoxicity assays, this CIB-based method offered distinct advantages in real-time kinetic measurement which provided a comprehensive understanding of cellular responses for the entire duration of the experiment and prediction of the potential mechanism of action of a given treatment. The time-dependent cell response profiles provided valid evidences for optimization of cell number per well, cell quality control, and identification of the optimal time points for compound treatment and endpoint assays. According to the time dependent IC50 values, the CIB could provide dynamic information that can be used to identify maximum toxicity of cigarette smoke and reversibility of the toxic effects which are difficult to achieve by the endpoint assays. The comparative IC50 values indicated that the as-developed biosensor offered analytical results in good consistency with the commonly used NRU method. The features of the CIB-based cytotoxicity assay, such as no cell labeling, automatic detection, and easy operation, give this assay potential to become routine setting for evaluating the cytotoxicity of CSCs.
authors.affiliation: China National Tobacco Quality Supervision & Test Center, Zhengzhou 450001, PR China. hunny_ch@163.com
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Cui
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Jiang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Pang
authors.forename: Gang-Ling
authors.initials: GL
authors.lastname: Tang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Hou
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Hu
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D020128:Inhibitory Concentration 50; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
chemical_list: D003470:Culture Media; D012906:Smoke; D004121:Dimethyl Sulfoxide