title: Development of a simple liquid chromatography-tandem mass spectrometry method for multiresidue determination of antifungal drugs in chicken tissues.
abstract: A method involving LC coupled with MS/MS (LC/MS/MS) was designed for simultaneous quantification of 10 antifungal drugs (voriconazole, griseofulvin, clotrimazole, bifonazole, econazole, ketoconazole, itraconazole, miconazole, terconazole, and fluconazole) in the liver and muscles of chickens. Homogenized tissue samples were extracted with acetonitrile and subsequently underwent freezing-delipidation. A Waters Acquity Ultra Performance LC BEH C18 column was used to separate the analytes, coupled with MS/MS using an electrospray ionization source. The accuracy of the method was confirmed with a mean recovery of 71-121%, and acceptable coefficients of variation (4-23%, n = 6). The detection capability of these compounds in two different matrixes was 0.50-2.82 microg/kg. This method can be applied for the screening and confirmation of target antifungal drugs in chicken tissues.
authors.affiliation: Beijing Center for Disease Control and Prevention, Beijing 100013, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
authors.forename: Yongning
authors.initials: Y
authors.lastname: Wu
authors.forename: Hejun
authors.initials: H
authors.lastname: Duan
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000032:analysis*; D002138:Calibration; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D000935:Antifungal Agents; D007202:Indicators and Reagents; D019155:Veterinary Drugs