title: [VEGF gene expression in transfected human multipotent stromal cells].
abstract: Dynamics of VEGF gene expression in transfected multipotent stromal cells from adipose tissue was examined using electroporation and lipofection. Differences in the potency and dynamics of plasmid elimination (up to day 9) between cell cultures were observed. All cultures were divided into fast and slow plasmid-eliminating ones. Interculture differences in VEGF expression were detected. The possibility of a 5-6-fold increase of VEGF expression was shown. There were no differences in transfection potency, plasmid elimination dynamics, and VEGF expression after transfection by both nonviral methods.
authors.forename: S A
authors.initials: SA
authors.lastname: Smirnikhina
authors.forename: A V
authors.initials: AV
authors.lastname: Lavrov
authors.forename: N P
authors.initials: NP
authors.lastname: Bochkov
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002478:Cells, Cultured; D018274:Electroporation; D015870:Gene Expression; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D010714:Phosphatidylethanolamines; D010957:Plasmids; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014161:Transduction, Genetic; D014162:Transfection / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D010714:Phosphatidylethanolamines; D042461:Vascular Endothelial Growth Factor A; C020888:1,2-dielaidoylphosphatidylethanolamine