title: Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material.
abstract: Environmental particulate matter (PM) pollutants adversely affect human health, but the molecular basis is poorly understood. The ion channel transient receptor potential vanilloid-1 (TRPV1) has been implicated as a sensor for environmental PM and a mediator of adverse events in the respiratory tract. The objectives of this study were to determine whether TRPV1 can distinguish chemically and physically unique PM that represents important sources of air pollution; to elucidate the molecular basis of TRPV1 activation by PM; and to ascertain the contributions of TRPV1 to human lung cell and mouse lung tissue responses exposed to an insoluble PM agonist, coal fly ash (CFA1). The major findings of this study are that TRPV1 is activated by some, but not all of the prototype PM materials evaluated, with rank-ordered responses of CFA1 > diesel exhaust PM > crystalline silica; TRP melastatin-8 is also robustly activated by CFA1, whereas other TRP channels expressed by airway sensory neurons and lung epithelial cells that may also be activated by CFA1, including TRPs ankyrin 1 (A1), canonical 4α (C4α), M2, V2, V3, and V4, were either slightly (TRPA1) or not activated by CFA1; activation of TRPV1 by CFA1 occurs via cell surface interactions between the solid components of CFA1 and specific amino acid residues of TRPV1 that are localized in the putative pore-loop region; and activation of TRPV1 by CFA1 is not exclusive in mouse lungs but represents a pathway by which CFA1 affects the expression of selected genes in lung epithelial cells and airway tissue.
authors.affiliation: Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: Cassandra E
authors.initials: CE
authors.lastname: Deering-Rice
authors.forename: Mark E
authors.initials: ME
authors.lastname: Johansen
authors.forename: Jessica K
authors.initials: JK
authors.lastname: Roberts
authors.forename: Karen C
authors.initials: KC
authors.lastname: Thomas
authors.forename: Erin G
authors.initials: EG
authors.lastname: Romero
authors.forename: Jeewoo
authors.initials: J
authors.lastname: Lee
authors.forename: Garold S
authors.initials: GS
authors.lastname: Yost
authors.forename: John M
authors.initials: JM
authors.lastname: Veranth
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Reilly
mesh_terms: D001483:Base Sequence; D002461:Cell Line, Transformed; D060729:Coal Ash / Q000633:toxicity*; D017931:DNA Primers; D020869:Gene Expression Profiling; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000502:physiology*
chemical_list: D060729:Coal Ash; D017931:DNA Primers; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human