title: Heavy metal ion inhibition studies of human, sheep and fish α-carbonic anhydrases.
abstract: Carbonic anhydrases (CAs, EC 4.2.1.1) were purified from sheep kidney (sCA IV), from the liver of the teleost fish Dicentrarchus labrax (dCA) and from human erythrocytes (hCA I and hCA II). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The kinetic parameters of these enzymes were determined for their esterase activity with 4-nitrophenyl acetate as substrate. The following metal ions, Pb(2+), Co(2+), Hg(2+), Cd(2+), Zn(2+), Se(2+), Cu(2+), Al(3+) and Mn(3+) showed inhibitory effects on these enzymes. The tested metal ions inhibited these CAs competitively in the low milimolar/submillimolar range. The susceptibility to various cations inhibitors differs significantly between these vertebrate α-CAs and is probably due to their binding to His64 or the histidine cluster.
authors.affiliation: Chemistry Department, Science Faculty, Atatürk University, Erzurum, Turkey.
authors.forename: Ramazan
authors.initials: R
authors.lastname: Demirdağ
authors.forename: Emrah
authors.initials: E
authors.lastname: Yerlikaya
authors.forename: Murat
authors.initials: M
authors.lastname: Şentürk
authors.forename: Ö İrfan
authors.initials: Öİ
authors.lastname: Küfrevioğlu
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
mesh_terms: D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000302:isolation & purification / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000201:enzymology; D005399:Fishes; D006801:Humans; D007477:Ions / Q000737:chemistry / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D019216:Metals, Heavy / Q000737:chemistry / Q000494:pharmacology*; D012756:Sheep; D013329:Structure-Activity Relationship
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D007477:Ions; D019216:Metals, Heavy; D002256:Carbonic Anhydrases