title: Bioreactor performance and methanogenic population dynamics in a low-temperature (5-18 °C) anaerobic fixed-bed reactor.
abstract: The effect of temperature on the functionality of microbial community structure in a low temperature, anaerobic fixed-bed reactor was studied by decreasing the operating temperature from 18 °C to 5 °C. The reactor was productive within 20 days and produced stable methane content in biogas (above 77%) throughout the trial period. At 17 °C and 15 °C, chemical oxygen demand (COD) removal efficiency and biogas production of reactor were significantly reduced. These might be temperature thresholds when fixed-bed reactors are operated under low temperatures. The methanogen community composition was analyzed using 16S rRNA gene clone library screening and quantitative PCR. At low ambient temperatures, Methanomicrobiales were dominant methanogens, and they preferentially adhered to the carbon fiber carrier. The results indicated that 16S rRNA levels of Methanomicrobiales and Methanosaetaceae in adhering sludge were higher than in deposited sludge, and they all contributed to the efficient performance of the fixed-bed reactor at low operating temperatures.
authors.affiliation: College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.forename: Wanbin
authors.initials: W
authors.lastname: Zhu
authors.forename: Can
authors.initials: C
authors.lastname: Tang
authors.forename: Yali
authors.initials: Y
authors.lastname: Suo
authors.forename: Lijuan
authors.initials: L
authors.lastname: Gao
authors.forename: Xufeng
authors.initials: X
authors.lastname: Yuan
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Wang
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000693:Anaerobiosis / Q000502:physiology; D001421:Bacteria, Anaerobic / Q000145:classification / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D019149:Bioreactors / Q000382:microbiology*; D003080:Cold Temperature; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019605:Euryarchaeota / Q000145:classification* / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D008697:Methane