title: Expression of the novel human gene, UBE2Q1, in breast tumors.
abstract: The novel human gene, designated ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) maps to chromosome 1q21.3. The gene has an open reading frame corresponding to 422 amino acids and contains a RWD domain and an E2 ubiquitin conjugating enzyme domain. Here, we investigated the expression levels of both mRNA and protein of UBE2Q1 gene in cancerous versus normal parts of breast specimens from 26 patients. Real-time PCR data showed that the relative expression level of UBE2Q1 mRNA was significantly greater in cancers than in non-cancerous tissues of breast specimens (Mean ± SEM, 0.064 ± 0.015 for cancers and 0.026 ± 0.01 for noncancerous tissues, P < 0.05 Mann-Whitney test). A rabbit polyclonal antibody was generated against an amino acid sequence predicted from the DNA sequence of UBE2Q1 gene. This antibody was used to perform Western blotting on 21 cases in our cohort of breast specimens. Thus, 13 (61.904%) of the cases showed an increase in the UBE2Q1 immunoreactivity in their cancerous tissues as compared with the corresponding normal tissues. This result along with the real-time PCR data shows that the novel human gene, UBE2Q1, is expressed in human breast and may have implications for pathogenesis of breast cancer.
authors.affiliation: Department of Biochemistry, Shiraz University of Medical Sciences, P.O. Box 1167, Shiraz, Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Seghatoleslam
authors.forename: Mohsen
authors.initials: M
authors.lastname: Nikseresht
authors.forename: Sayed Mohammad
authors.initials: SM
authors.lastname: Shafiee
authors.forename: Ahmad
authors.initials: A
authors.lastname: Monabati
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Namavari
authors.forename: Abdolrassul
authors.initials: A
authors.lastname: Talei
authors.forename: Akbar
authors.initials: A
authors.lastname: Safaei
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Owji
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology; D001940:Breast / Q000201:enzymology / Q000473:pathology; D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000912:Antibodies, Neoplasm; D012333:RNA, Messenger; D044763:Ubiquitin-Conjugating Enzymes