title: Detection of bioactive exometabolites produced by the filamentous marine cyanobacterium Geitlerinema sp.
abstract: Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21 days) at low volumes of culture media (300 mL) required for identification of two selected bioactive compounds: 4,4'-dihydroxybiphenyl and harmane.
authors.affiliation: UFT-Centre for Environmental Research and Sustainable Technology, University of Bremen, Leobener Straße UFT, 28359, Bremen, Germany. uacocjunior@googlemail.com
authors.forename: Nelson H
authors.initials: NH
authors.lastname: Caicedo
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kumirska
authors.forename: Jennifer
authors.initials: J
authors.lastname: Neumann
authors.forename: Stefan
authors.initials: S
authors.lastname: Stolte
authors.forename: Jorg
authors.initials: J
authors.lastname: Thöming
mesh_terms: D001688:Biological Products / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D000458:Cyanobacteria / Q000145:classification / Q000378:metabolism*; D006247:Harmine / Q000031:analogs & derivatives; D009792:Oceans and Seas; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D001688:Biological Products; D001713:Biphenyl Compounds; D006247:Harmine; C005010:harman; C027439:4,4'-dihydroxybiphenyl