title: Biofunctionalized zinc oxide field effect transistors for selective sensing of riboflavin with current modulation.
abstract: Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3' terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels.
authors.affiliation: 711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Labs, Wright Patterson Air Force Base, OH 45433, USA. Joshua.Hagen@wpafb.af.mil
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Hagen
authors.forename: Sang N
authors.initials: SN
authors.lastname: Kim
authors.forename: Burhan
authors.initials: B
authors.lastname: Bayraktaroglu
authors.forename: Kevin
authors.initials: K
authors.lastname: Leedy
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Chávez
authors.forename: Nancy
authors.initials: N
authors.lastname: Kelley-Loughnane
authors.forename: Rajesh R
authors.initials: RR
authors.lastname: Naik
authors.forename: Morley O
authors.initials: MO
authors.lastname: Stone
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D000075163:Metallocenes; D049329:Nanostructures; D012256:Riboflavin / Q000032:analysis*; D014173:Transistors, Electronic; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005296:Ferrous Compounds; D000075163:Metallocenes; D015034:Zinc Oxide; D012256:Riboflavin; C004998:ferrocene