title: Sequence and structural features of binding site residues in protein-protein complexes: comparison with protein-nucleic acid complexes.
abstract: BACKGROUND
  Protein-protein interactions are important for several cellular processes. Understanding the mechanism of protein-protein recognition and predicting the binding sites in protein-protein complexes are long standing goals in molecular and computational biology.
  
  
  METHODS
  We have developed an energy based approach for identifying the binding site residues in protein-protein complexes. The binding site residues have been analyzed with sequence and structure based parameters such as binding propensity, neighboring residues in the vicinity of binding sites, conservation score and conformational switching.
  
  
  RESULTS
  We observed that the binding propensities of amino acid residues are specific for protein-protein complexes. Further, typical dipeptides and tripeptides showed high preference for binding, which is unique to protein-protein complexes. Most of the binding site residues are highly conserved among homologous sequences. Our analysis showed that 7% of residues changed their conformations upon protein-protein complex formation and it is 9.2% and 6.6% in the binding and non-binding sites, respectively. Specifically, the residues Glu, Lys, Leu and Ser changed their conformation from coil to helix/strand and from helix to coil/strand. Leu, Ser, Thr and Val prefer to change their conformation from strand to coil/helix.
  
  
  CONCLUSIONS
  The results obtained in this study will be helpful for understanding and predicting the binding sites in protein-protein complexes.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, Tamilnadu, India. gromiha@iitm.ac.in.
authors.forename: M Michael
authors.initials: MM
authors.lastname: Gromiha
authors.forename: N
authors.initials: N
authors.lastname: Saranya
authors.forename: S
authors.initials: S
authors.lastname: Selvaraj
authors.forename: B
authors.initials: B
authors.lastname: Jayaram
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Fukui