title: Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity.
abstract: Interleukin 4 (IL-4) and IL-13 are critical for responses to parasitic helminthes. We used genetically engineered reporter mice to assess the temporal and spatial production of these cytokines in vivo. In lymph nodes, IL-4, but not IL-13, was made by follicular helper T cells (T(FH) cells). In contrast, tissue type 2 helper T cells (T(H)2 cells) produced both cytokines. There was also divergent production of IL-4 and IL-13 among cells of the innate immune system, whereby basophils produced IL-4, whereas innate helper type 2 cells (Ih2 cells) produced IL-13. IL-13 production by T(H)2 and Ih2 cells was dependent on the transcription factor GATA-3, which was present in large amounts in these cells, and in contrast to the small amount of GATA-3 in T(FH) cells and basophils. The distinct localization and cellular expression of IL-4 and IL-13 explains their unique roles during allergic immunity.
authors.affiliation: Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA.
authors.forename: Hong-Erh
authors.initials: HE
authors.lastname: Liang
authors.forename: R Lee
authors.initials: RL
authors.lastname: Reinhardt
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Bando
authors.forename: Brandon M
authors.initials: BM
authors.lastname: Sullivan
authors.forename: I-Cheng
authors.initials: IC
authors.lastname: Ho
authors.forename: Richard M
authors.initials: RM
authors.lastname: Locksley
mesh_terms: D000818:Animals; D001491:Basophils / Q000276:immunology; D050990:GATA3 Transcription Factor / Q000378:metabolism; D015870:Gene Expression; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology*; D007113:Immunity, Innate; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D021381:Protein Transport; D050800:STAT6 Transcription Factor / Q000378:metabolism; D017206:Strongylida Infections / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D050990:GATA3 Transcription Factor; D018793:Interleukin-13; D050800:STAT6 Transcription Factor; D015847:Interleukin-4