title: Isotope-based quantitation of uptake, release, and metabolism of glutamate and glucose in cultured astrocytes.
abstract: Protocols are described for measurement in primary cultures of astrocytes of unidirectional fluxes of glutamate (influx and efflux), glutamate metabolism to glutamine or CO(2), glucose influx, glycolysis, pyruvate dehydrogenation, oxidative metabolism of glucose, pyruvate carboxylation, glycogen synthesis, and glycogenolysis. References are made to the in vivo situation, and the importance of using metabolically competent cultures is emphasized.
authors.affiliation: College of Basic Medical Sciences, China Medical University, Shenyang, People's Republic of China. leifhertz@xplornet.ca
authors.forename: Leif
authors.initials: L
authors.lastname: Hertz
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002952:Citric Acid Cycle / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology; D007700:Kinetics; D051379:Mice; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D018698:Glutamic Acid; D019289:Pyruvic Acid; D005947:Glucose