title: Resveratrol prevents dexamethasone-induced expression of the muscle atrophy-related ubiquitin ligases atrogin-1 and MuRF1 in cultured myotubes through a SIRT1-dependent mechanism.
abstract: Resveratrol (3,5,4'-trihydroxystilbene) has been ascribed multiple beneficial biological effects but the influence of resveratrol on glucocorticoid-induced muscle atrophy is not known. We examined the effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression, FOXO1 acetylation, protein degradation and atrophy in cultured L6 myotubes. In addition, the role of the deacetylase SIRT1 in the effects of resveratrol was determined by transfecting myotubes with SIRT1 siRNA. The catabolic effects of dexamethasone were prevented by resveratrol and the protective effects of resveratrol on dexamethasone-induced atrogin-1 and MuRF1 expression were abolished in myotubes transfected with SIRT1 siRNA. Results suggest that resveratrol can prevent glucocorticoid-induced muscle wasting and that this effect is at least in part SIRT1-dependent.
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, United States.
authors.forename: Nima
authors.initials: N
authors.lastname: Alamdari
authors.forename: Zaira
authors.initials: Z
authors.lastname: Aversa
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Castillero
authors.forename: Aniket
authors.initials: A
authors.lastname: Gurav
authors.forename: Victoria
authors.initials: V
authors.lastname: Petkova
authors.forename: Steven
authors.initials: S
authors.lastname: Tizio
authors.forename: Per-Olof
authors.initials: PO
authors.lastname: Hasselgren
mesh_terms: D000107:Acetylation; D000818:Animals; D002460:Cell Line; D003907:Dexamethasone / Q000378:metabolism* / Q000494:pharmacology; D051858:Forkhead Transcription Factors / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009124:Muscle Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D000077185:Resveratrol; D044843:SKP Cullin F-Box Protein Ligases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000071161:Forkhead Box Protein O1
chemical_list: D003907:Dexamethasone; D051858:Forkhead Transcription Factors; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D000077185:Resveratrol; D044843:SKP Cullin F-Box Protein Ligases; D056564:Sirtuin 1; D013267:Stilbenes; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1; C486692:Fbxo32 protein, rat; C499086:Trim63 protein, rat; C488368:Sirt1 protein, rat