title: Cytogenetic analysis of de novo CD5-positive diffuse large B-cell lymphoma.
abstract: OBJECTIVE
  De novo CD5-positive diffuse large B cell lymphoma (CD5+DLBL) is a subtype of DLBL with poor clinical outcome. To investigate the cytogenetic pathogenesis of CD5+DLBL, we analyzed the chromosomal findings of 18 patients with CD5+DLBL.
  
  
  METHODS
  Tumor cells were cultured and metaphase was captured by colchicine exposure. Using trypsin-Giemsa banding, the chromosomes were analyzed according to the International System for Human Cytogenetic Nomenclature.
  
  
  RESULTS
  Metaphase was acquired from 12 patients. Normal karyotypes were seen in two patients and abnormal karyotypes in the remaining 10. The numbers of chromosomes ranged from 45 to 90. Gain, loss and rearrangements of various chromosomes were seen. Frequent breakpoints were located at chromosome 1 band p13, 3q27, 6q13, 7q32, 14q32, 18q21 and 19q13. There was no diagnosis-specific abnormality. A relationship between chromosomal findings and clinical outcomes such as involved site, relapse or survival, was not observed.
  
  
  CONCLUSIONS
  Since previous and the present studies on the chromosomal analysis of CD5+DLBL are also contradictory, more detailed comprehensive genetic analysis appears to be needed to elucidate the biological mechanisms of CD5+DLBL.
authors.affiliation: Department of Hematology, Aiseikai Yamashina Hospital, Department of Hematology/Oncology, Kyoto, Japan. hp@aiseikaihp.or.jp
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kaneko
authors.forename: Kazuho
authors.initials: K
authors.lastname: Shimura
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horiike
authors.forename: Junya
authors.initials: J
authors.lastname: Kuroda
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Shohei
authors.initials: S
authors.lastname: Yokota
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishida
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohkawara
authors.forename: Masafumi
authors.initials: M
authors.lastname: Taniwaki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018956:CD5 Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D059785:Karyotype; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016019:Survival Analysis
chemical_list: D018956:CD5 Antigens