title: NCO-sP(EO-stat-PO) coatings on gold sensors--a QCM study of hemocompatibility.
abstract: The reliability of implantable blood sensors is often hampered by unspecific adsorption of plasma proteins and blood cells. This not only leads to a loss of sensor signal over time, but can also result in undesired host vs. graft reactions. Within this study we evaluated the hemocompatibility of isocyanate conjugated star shaped polytheylene oxide-polypropylene oxide co-polymers NCO-sP(EO-stat-PO) when applied to gold surfaces as an auspicious coating material for gold sputtered blood contacting sensors. Quartz crystal microbalance (QCM) sensors were coated with ultrathin NCO-sP(EO-stat-PO) films and compared with uncoated gold sensors. Protein resistance was assessed by QCM measurements with fibrinogen solution and platelet poor plasma (PPP), followed by quantification of fibrinogen adsorption. Hemocompatibility was tested by incubation with human platelet rich plasma (PRP). Thrombin antithrombin-III complex (TAT), β-thromboglobulin (β-TG) and platelet factor 4 (PF4) were used as coagulation activation markers. Furthermore, scanning electron microscopy (SEM) was used to visualize platelet adhesion to the sensor surfaces. Compared to uncoated gold sensors, NCO-sP(EO-stat-PO) coated sensors revealed significant better resistance against protein adsorption, lower TAT generation and a lower amount of adherent platelets. Moreover, coating with ultrathin NCO-sP(EO-stat-PO) films creates a cell resistant hemocompatible surface on gold that increases the chance of prolonged sensor functionality and can easily be modified with specific receptor molecules.
authors.affiliation: Clinical Research Laboratory, Department of Congenital & Pediatric Cardiac Surgery, Children's University Hospital, Tuebingen University, Calwerstr 7/1, 72076 Tuebingen, Germany. stefan.sinn@thg-lab.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Sinn
authors.forename: Mirjam
authors.initials: M
authors.lastname: Eichler
authors.forename: Lothar
authors.initials: L
authors.lastname: Müller
authors.forename: Daniel
authors.initials: D
authors.lastname: Bünger
authors.forename: Jürgen
authors.initials: J
authors.lastname: Groll
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ziemer
authors.forename: Frank
authors.initials: F
authors.lastname: Rupp
authors.forename: Hinnak
authors.initials: H
authors.lastname: Northoff
authors.forename: Jürgen
authors.initials: J
authors.lastname: Geis-Gerstorfer
authors.forename: Frank K
authors.initials: FK
authors.lastname: Gehring
authors.forename: Hans P
authors.initials: HP
authors.lastname: Wendel
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D005340:Fibrinogen / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D010949:Plasma / Q000737:chemistry; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D006046:Gold; D005340:Fibrinogen