title: Targeted detection of 65 kDa heat shock protein gene in endometrial biopsies for reliable diagnosis of genital tuberculosis.
abstract: OBJECTIVE
  To evaluate the clinical utility of PCR compared with other available diagnostic modalities in prompt diagnosis of female genital tuberculosis causing infertility.
  
  
  METHODS
  Prospective case-controlled trial. Premenstrual endometrial biopsy specimens were collected from 150 infertile women of reproductive age group suspected of having genital tuberculosis. All patients underwent diagnostic endoscopy (laparoscopy and hysteroscopy) and the samples obtained were subjected to microscopy, culture by the BACTEC 460 TB System, histopathology and polymerase chain reaction (PCR) for detection of 165 bp region of 65 kDa gene of Mycobacterium tuberculosis. The results were correlated with the laparoscopic findings.
  
  
  RESULTS
  While the laparoscopy/hysteroscopy findings were indicative of tuberculosis in 12.6% of cases, 14.6% of the specimens showed evidence of 65 kDa gene of M. tuberculosis and only 3.33%, 1.33% and 0.66% were positive by culture, smear and histopathology, respectively.
  
  
  CONCLUSIONS
  Since laparoscopy, hysteroscopy other endoscopic procedures are associated with operative risks and may cause flaring of infection, and other conventional laboratory tests including histopathology have poor sensitivity, PCR-based detection of 65 kDa gene of M. tuberculosis in endometrial biopsy specimens could be a promising molecular diagnostic technique compared to conventional methods of diagnosis.
authors.affiliation: IVF and Reproductive Biology Centre, Department of Obstetrics and Gynaecology, Maulana Azad Medical College, New Delhi 110002, India. drsprasad08@gmail.com
authors.forename: Sudha
authors.initials: S
authors.lastname: Prasad
authors.forename: Megha
authors.initials: M
authors.lastname: Singhal
authors.forename: Sanjay S
authors.initials: SS
authors.lastname: Negi
authors.forename: Sunil
authors.initials: S
authors.lastname: Gupta
authors.forename: Supriya
authors.initials: S
authors.lastname: Singh
authors.forename: Devendra S
authors.initials: DS
authors.lastname: Rawat
authors.forename: Arvind
authors.initials: A
authors.lastname: Rai
mesh_terms: D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001706:Biopsy; D016022:Case-Control Studies; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006801:Humans; D007194:India; D007247:Infertility, Female / Q000209:etiology; D025202:Molecular Diagnostic Techniques; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014384:Tuberculosis, Female Genital / Q000175:diagnosis* / Q000382:microbiology* / Q000503:physiopathology; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium