title: [Immunogenic properties and molecular-genetic features of native and inactivated Chlamydia trachomatis MT-2A serovariant D strain].
abstract: AIM. Study of antibody genesis after introduction of native and inactivated Chlamydia trachomatis MT-2A strain into Balb/c mice and analysis of molecular-genetic features of omp A gene that is responsible for its immunogenicity.
  
  
  METHODS
  Chlamydia infection was modeled in Balb/c mice by using human isolated C. trachomatis serovariant D.
  
  
  RESULTS
  After the introduction of this strain, 5 days after the infection of mice A, M and G class antibodies including G class antibodies against chlamydia heat shock protein are induced. Studies of molecular-genetic features of the isolated C. trachomatis MT-2A strain showed presence of 2 nucleotide mutations in ompAgene. A synonymous substitution of cytosine to thymine in position 574 (CD) that does not result in amino acid substitution in the protein was registered. A non-synonymous substitution of thymine to guanine was present in position 843 (VDIII), that is most probably one of the main reasons of the registered higher immunogenicity of C. trachomatis MT-2A serovariant D strain.
  
  
  CONCLUSIONS
  Further studies of C. trachomatis MT-2A serovariant D strain are promising, but do not exclude search of other immunogenic strains for the generation of agents for specific prophylaxis and immunotherapy of chlamydiosis.
authors.forename: N N
authors.initials: NN
authors.lastname: Poleshchuk
authors.forename: L V
authors.initials: LV
authors.lastname: Rubanik
authors.forename: N N
authors.initials: NN
authors.lastname: Kapitulets
authors.forename: S A
authors.initials: SA
authors.lastname: Kostiuk
authors.forename: E V
authors.initials: EV
authors.lastname: Barkovskiĭ
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002690:Chlamydia Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004269:DNA, Bacterial; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D007136:Immunoglobulins; C078167:OMPA outer membrane proteins