title: Cross-validation of the structure of a transiently formed and low populated FF domain folding intermediate determined by relaxation dispersion NMR and CS-Rosetta.
abstract: We have recently reported the atomic resolution structure of a low populated and transiently formed on-pathway folding intermediate of the FF domain from human HYPA/FBP11 [Korzhnev, D. M.; Religa, T. L.; Banachewicz, W.; Fersht, A. R.; Kay, L.E. Science 2011, 329, 1312-1316]. The structure was determined on the basis of backbone chemical shift and bond vector orientation restraints of the invisible intermediate state measured using relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy that were subsequently input into the database structure determination program, CS-Rosetta. As a cross-validation of the structure so produced, we present here the solution structure of a mimic of the folding intermediate that is highly populated in solution, obtained from the wild-type domain by mutagenesis that destabilizes the native state. The relaxation dispersion/CS-Rosetta structures of the intermediate are within 2 Å of those of the mimic, with the nonnative interactions in the intermediate also observed in the mimic. This strongly confirms the structure of the FF domain folding intermediate, in particular, and validates the use of relaxation dispersion derived restraints in structural studies of invisible excited states, in general.
authors.affiliation: Department of Molecular Genetics, The University of Toronto, Toronto, Ontario M5S1A8, Canada.
authors.forename: Julia
authors.initials: J
authors.lastname: Barette
authors.forename: Algirdas
authors.initials: A
authors.lastname: Velyvis
authors.forename: Tomasz L
authors.initials: TL
authors.lastname: Religa
authors.forename: Dmitry M
authors.initials: DM
authors.lastname: Korzhnev
authors.forename: Lewis E
authors.initials: LE
authors.lastname: Kay
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D030562:Databases, Protein; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D002352:Carrier Proteins; C491404:PRPF40A protein, human