title: Biosynthesis of gold nanoparticles using chloroplasts.
abstract: In this paper, a new method of one-pot biosynthesizing of gold nanoparticles (GNPs), using chloroplasts as reductants and stabilizers is reported. The as-prepared GNPs were characterized by ultraviolet visible spectroscopy, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the GNPs was evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against gastric mucous cell line GES-1 and gastric cancer cell line MGC-803. Rhodamine 6G as a Raman probe was used for investigating surface-enhanced Raman spectroscopy (SERS) enhancement of GNPs. The transmission electron microscopy results indicated that the GNPs were spherical in structure and almost 20 nm in diameter. Ultraviolet visible spectroscopy exhibited an absorption peak at 545 nm. The GNPs exhibited high crystallinity, with the (111) plane as the predominant orientation, clarified by X-ray powder diffraction. In addition, a potential mechanism was proposed to interpret the formation process of GNPs, mainly based on the analysis of FTIR results. The FTIR spectrum confirmed that the GNPs were carried with N-H groups. Toxicological assays of as-prepared GNPs revealed that the green GNPs were nontoxic. SERS analysis revealed that the GNPs without any treatment could substantially enhance the Raman signals of rhodamine 6G. The Raman enhancement factor was calculated to be nearly 10(10) orders of magnitude. In conclusion, the GNPs with good biocompatibility and excellent SERS effect were successfully synthesized using chloroplasts. These biogenetic GNPs have great potential for ultrasensitive detection of biomarkers in vitro and in vivo based on SERS.
authors.affiliation: Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China.
authors.forename: Yi Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.forename: Guo
authors.initials: G
authors.lastname: Gao
authors.forename: Yi Fei
authors.initials: YF
authors.lastname: Kong
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhi
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.forename: Xue Qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Da Xiang
authors.initials: DX
authors.lastname: Cui
mesh_terms: D001709:Biotechnology / Q000379:methods*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002736:Chloroplasts / Q000378:metabolism*; D006046:Gold / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D055772:Green Chemistry Technology / Q000379:methods; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D012235:Rhodamines / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D029921:Trifolium / Q000378:metabolism
chemical_list: D012235:Rhodamines; D013778:Tetrazolium Salts; D013844:Thiazoles; C026188:rhodamine 6G; D006046:Gold; C022616:thiazolyl blue