title: Analysis of altered proteins related to blast crisis in chronic myeloid leukemia by proteomic study.
abstract: BACKGROUND
  Chromic myeloid leukemia (CML) blast crisis (BC) and imatinib (IM) resistance is a significant barrier to the effective treatment of the disease.
  
  
  METHODS
  Expression profiles of differential proteins were identified, and new biomarkers or pathways related to BC in CML were screened through proteomic analysis. Total proteins from primary bone marrow cells of CML patients in chronic phase (CP) and BC were separated via two-dimensional (2D) polyacrylamide gel electrophoresis and then analyzed by imagemaster 5.0 software to detect differential protein spots which were already identified by mass spectrometry. Based on the variation of the whole expression profile, some key proteins were picked out for Western blot to confirm the accuracy of proteomics data. Moreover, related signal pathways involving those proteins were investigated.
  
  
  RESULTS
  The result indicated that thirteen protein points between CML-CP and CML-BC were successfully determined. Results from Western blot of RhoA, hnRNPK, ANXA1, PSMB4, and LTA4H were similar to those from 2D polyacrylamide gel electrophoresis. Most of those proteins were involved in the proteosome pathway and the small G-protein pathway.
  
  
  CONCLUSIONS
  A group of proteins associated with BC can be obtained and the result of this study might provide clues for further research.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Jin
authors.forename: Q
authors.initials: Q
authors.lastname: DU
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Yao
authors.forename: B
authors.initials: B
authors.lastname: Huang
authors.forename: N
authors.initials: N
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Luo
authors.forename: X
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D017302:Annexins / Q000378:metabolism; D001752:Blast Crisis / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012261:Ribonucleoproteins / Q000378:metabolism; D055815:Young Adult; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: C121387:ANXA10 protein, human; D017302:Annexins; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012261:Ribonucleoproteins; C066737:RHOA protein, human; C072074:HNRNPK protein, human; C108898:PSMB4 protein, human; D046988:Proteasome Endopeptidase Complex; D020742:rhoA GTP-Binding Protein