title: Effects of Al(III) and nano-Al13 species on malate dehydrogenase activity.
abstract: The effects of different aluminum species on malate dehydrogenase (MDH) activity were investigated by monitoring amperometric i-t curves for the oxidation of NADH at low overpotential using a functionalized multi-wall nanotube (MWNT) modified glass carbon electrode (GCE). The results showed that Al(III) and Al(13) can activate the enzymatic activity of MDH, and the activation reaches maximum levels as the Al(III) and Al(13) concentration increase. Our study also found that the effects of Al(III) and Al(13) on the activity of MDH depended on the pH value and aluminum speciation. Electrochemical and circular dichroism spectra methods were applied to study the effects of nano-sized aluminum compounds on biomolecules.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Material Science, Nanjing Normal University, Nanjing, China. yangxiaodi@njnu.edu.cn
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Cai
authors.forename: Yu
authors.initials: Y
authors.lastname: Peng
authors.forename: Huihui
authors.initials: H
authors.lastname: Li
authors.forename: Rong Fu
authors.initials: RF
authors.lastname: Chen
authors.forename: Ren Fang
authors.initials: RF
authors.lastname: Shen
mesh_terms: D000534:Alum Compounds / Q000737:chemistry; D000535:Aluminum / Q000737:chemistry*; D017607:Aluminum Compounds / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D002942:Circular Dichroism; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005898:Glass; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000378:metabolism*; D009243:NAD / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry; D062907:Oxaloacetic Acid / Q000737:chemistry; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000534:Alum Compounds; D017607:Aluminum Compounds; D009243:NAD; D062907:Oxaloacetic Acid; C041524:aluminum sulfate; D002244:Carbon; D000535:Aluminum; D008291:Malate Dehydrogenase