title: Collagen-immobilized patch for repairing small tympanic membrane perforations: in vitro and in vivo assays.
abstract: Tympanic membrane (TM) perforation is still one of the most common otology complications. New designs of biomaterials, and lately tissue-engineered composites and grafts, have thoroughly revolutionized the management of TM perforation. In this study, we examined a biologically modified collagen-immobilized polydimethyl siloxane patch to repair TM perforation. In vitro potential of the aforementioned patch as a scaffold to support fibroblast cell growth and adhesion was assessed. An in vivo assay of the patch for initiating repair of TM perforations also was investigated. In vitro assay showed that the patch has significantly increased cell adhesion and growth in comparison with unmodified ones (p < 0.05). In vivo study also showed an overall closure rate of TM perforation of 70% and an average gain of 15.75 ± 4.29 dB in air-bone gap. This study shows that the preliminary in vivo evaluation of a modified siloxane patch in humans had promising results and is comparable to existing biomaterial patches.
authors.affiliation: ENT-Head and Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farhadi
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirzadeh
authors.forename: Atefeh
authors.initials: A
authors.lastname: Solouk
authors.forename: Alimohamad
authors.initials: A
authors.lastname: Asghari
authors.forename: Maryam
authors.initials: M
authors.lastname: Jalessi
authors.forename: Hadi
authors.initials: H
authors.lastname: Ghanbari
authors.forename: Parin
authors.initials: P
authors.lastname: Yazdanifard
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D023822:Tissue Engineering / Q000379:methods*; D019737:Transplants; D016896:Treatment Outcome; D014432:Tympanic Membrane / Q000473:pathology* / Q000601:surgery*; D018058:Tympanic Membrane Perforation / Q000601:surgery*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D003094:Collagen