title: Effect of a hyperlipidic diet rich in omegas 3, 6 and 9 on aberrant crypt formation in rat colonic mucosa.
abstract: OBJECTIVE
  To determine whether a hypercaloric and hyperlipidic diet enriched with polyunsaturated fatty acids influences the formation of aberrant crypt foci (ACF) in colonic mucosa of Wistar rats treated with azoxymethane (AOM).
  
  
  METHODS
  At eight weeks of life, the rats were assigned to four groups: Group I-standard diet (STD) not treated with AOM; Group II-hypercaloric and hyperlipidic diet (FED), not treated with AOM; Group III-STD, treated with AOM; Group IV-FED, treated with AOM. At 16 weeks, the animals were injected intraperitoneal with 0.9% saline solution (Group I and II) or AOM at 15 mg/Kg (Groups III and IV) once a week for two weeks. Fifteen weeks later, the animals were euthanized.
  
  
  RESULTS
  FED promoted weight gain in Groups II and IV compared to Groups I and III, respectively. The groups did not differ with regard to the total number of ACF. The Chi-square test revealed no predominance of the presence of foci with <4 crypts. However, foci with ≥5 crypts were proportionally more prevalent in Group III than in Group IV (p=0.043).
  
  
  CONCLUSIONS
  The administration of polyunsaturated fatty acids did not interfere with the formation of aberrant crypt foci, but reduced ACF multiplicity, exercising an attenuating effect on carcinogenesis.
authors.affiliation: Postgraduate Program in Surgery, Federal University of Ceara, Ceara, Brazil. idaliaburlamaqui87@hotmail.com
authors.forename: Idália Maria Brasil
authors.initials: IM
authors.lastname: Burlamaqui
authors.forename: Conceição Aparecida
authors.initials: CA
authors.lastname: Dornelas
authors.forename: José Telmo
authors.initials: JT
authors.lastname: Valença Júnior
authors.forename: Daniel Magalhães Coutinho
authors.initials: DM
authors.lastname: Mota
authors.forename: Francisco José Cabral
authors.initials: FJ
authors.lastname: Mesquita
authors.forename: Lara Burlamaqui
authors.initials: LB
authors.lastname: Veras
authors.forename: Paulo Roberto Leitão
authors.initials: PR
authors.lastname: Vasconcelos
authors.forename: Lusmar Veras
authors.initials: LV
authors.lastname: Rodrigues
mesh_terms: D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity; D003106:Colon / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000187:drug effects; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002273:Carcinogens; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D001397:Azoxymethane