title: Levels of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 °C overnight in Turkey.
abstract: BACKGROUND
  The aim of this study was to assess whether the quantities of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 °C for 24 hours are adequate for their intended purpose.
  
  
  METHODS
  Fresh-frozen plasma separated from whole blood after storage at 4 °C overnight (24 hours from donation) was compared with plasma prepared 8 hours after donation using a standard method. The amounts of factor VIII and factor IX obtained with the two methods were compared.
  
  
  RESULTS
  Compared to the levels of factor VIII and factor IX in plasma prepared within 8 hours of blood collection, the levels in plasma prepared after 24 hours of storage at 4 °C were 25% and 9% lower, respectively. Ninety percent of the factor VIII and 100% of the factor IX levels were above 0.5 IU/mL (standard haematology reference range) after 24 hours of storage.
  
  
  CONCLUSIONS
  These data suggest that there is good retention of coagulation factor activity in plasma produced from whole blood stored at 4 ºC for 24 hours and that such plasma would be an acceptable product for most patients requiring fresh-frozen plasma.
authors.affiliation: Department of Blood Bank, Tepecik Educational and Research Hospital, Izmir, Turkey. nevalagus@yahoo.com
authors.forename: Neval
authors.initials: N
authors.lastname: Agus
authors.forename: Nisel
authors.initials: N
authors.lastname: Yilmaz
authors.forename: Ayfer
authors.initials: A
authors.lastname: Colak
authors.forename: Fatma
authors.initials: F
authors.lastname: Liv
mesh_terms: D001780:Blood Coagulation Tests; D001793:Blood Preservation; D003080:Cold Temperature; D005164:Factor IX / Q000032:analysis* / Q000378:metabolism; D005169:Factor VIII / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D014421:Turkey
chemical_list: D005169:Factor VIII; D005164:Factor IX