title: Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers.
abstract: Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by self-assembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS-ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P < 0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Cheng
authors.forename: Qi Feng
authors.initials: QF
authors.lastname: Dang
authors.forename: Fang Kui
authors.initials: FK
authors.lastname: Ma
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Bum Keun
authors.initials: BK
authors.lastname: Kim
mesh_terms: D000284:Administration, Oral; D000333:Aeromonas / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000941:Antigens / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002347:Carps; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D008027:Light; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D012995:Solubility; D013314:Stress, Mechanical; D014867:Water / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D000941:Antigens; D001672:Biocompatible Materials; D004337:Drug Carriers; C504164:O-carboxymethylchitosan; D014867:Water; D048271:Chitosan