title: An atypical strain associated with congenital toxoplasmosis in Tunisia.
abstract: We report the identification and typing of a congenital toxoplasmosis case in a diabetic pregnant young woman living in Tunis. The Toxoplasma DNA extracted from amniotic fluid was detected by Real Time PCR and subjected to a multilocus genetic characterisation of the strain at markers: 3'SAG2, 5'SAG2, New SAG2, SAG3, GRA6, BTUB, APICO, PK1, KT850 and UPRT1. An atypical genotype of T.gondii with unusual genetic composition was revealed. It is the first time that an atypical strain has been associated with congenital toxoplasmosis in Africa. Atypical strains are associated with severe clinical manifestations so systematic genotyping should be investigated with the amniocentesis.
authors.affiliation: Laboratoire de Parasitologie, Institut Pasteur de Tunis, Tunis Belvedère, Tunisia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Boughattas
authors.forename: Rym Ben
authors.initials: RB
authors.lastname: Abdallah
authors.forename: Emna
authors.initials: E
authors.lastname: Siala
authors.forename: Karim
authors.initials: K
authors.lastname: Aoun
authors.forename: Aida
authors.initials: A
authors.lastname: Bouratbine
mesh_terms: D000328:Adult; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D011247:Pregnancy; D014122:Toxoplasma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000469:parasitology*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis / Q000469:parasitology*; D014416:Tunisia
chemical_list: D016054:DNA, Protozoan