title: Characterization of the third glutathione S-transferase gene involved in enantioselective cleavage of the β-aryl ether by Sphingobium sp. strain SYK-6.
abstract: The glutathione S-transferases, LigF and LigE, of Sphingobium sp. strain SYK-6 respectively play a role in cleavage of the β-aryl ether of (+)-(βS)-α-(2-methoxyphenoxy)-β-hydroxypropiovanillone (MPHPV) and (-)-(βR)-MPHPV. The ligP gene, which showed 59% similarity to ligE at the amino acid level, was isolated from SYK-6. LigP produced in Escherichia coli revealed enantioselectivity for (-)-(βR)-MPHPV, and ligE and ligP alone contributed to the degradation of (-)-(βR)-MPHPV in SYK-6.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tanamura
authors.forename: Tomokuni
authors.initials: T
authors.lastname: Abe
authors.forename: Naofumi
authors.initials: N
authors.lastname: Kamimura
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kasai
authors.forename: Shojiro
authors.initials: S
authors.lastname: Hishiyama
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Shinya
authors.initials: S
authors.lastname: Kajita
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Katayama
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
mesh_terms: D004987:Ethers / Q000737:chemistry*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D006841:Hydrocarbons, Aromatic / Q000737:chemistry* / Q000378:metabolism*; D042301:Sphingomonadaceae / Q000201:enzymology* / Q000235:genetics*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D004987:Ethers; D006841:Hydrocarbons, Aromatic; D005982:Glutathione Transferase