title: The Lymantria dispar IPLB-Ld652Y cell line transcriptome comprises diverse virus-associated transcripts.
abstract: The enhanced viral susceptibility of the gypsy moth (Lymantria dispar)-derived IPLB-Ld652Y cell line has made it a popular in vitro system for studying virus-related phenomena in the Lepidoptera. Using both single-pass EST sequencing and 454-based pyrosequencing, a transcriptomic library of 14,368 putatively unique transcripts (PUTs) was produced comprising 8,476,050 high-quality, informative bases. The gene content of the IPLB-Ld652Y transcriptome was broadly assessed via comparison with the NCBI non-redundant protein database, and more detailed functional annotation was inferred by comparison to the Swiss-Prot subset of UniProtKB. In addition to L. dispar cellular transcripts, a diverse array of both RNA and DNA virus-associated transcripts was identified within the dataset, suggestive of a high level of viral expression and activity in IPLB-Ld652Y cells. These sequence resources will provide a sound basis for developing testable experimental hypotheses by insect virologists, and suggest a number of avenues for potential research.
authors.affiliation: USDA-ARS Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, MD 20705, USA. Michael.Sparks@ars.usda.gov
authors.forename: Michael E
authors.initials: ME
authors.lastname: Sparks
authors.forename: Dawn E
authors.initials: DE
authors.lastname: Gundersen-Rindal
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D019476:Insect Proteins / Q000235:genetics*; D007304:Insect Viruses / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000821:virology*; D059467:Transcriptome; D014779:Virus Replication
chemical_list: D019476:Insect Proteins