title: Scaling up of ethanol production from sugar molasses using yeast immobilized with alginate-based MCM-41 mesoporous zeolite composite carrier.
abstract: Microporous and mesoporous zeolites, including ZSM-5, H-β, H-Y, and MCM-41, were modified with 3-aminopropyl-triethoxysilane (APTES), then inorganic fillers, such as abovementioned zeolites or mesoporous materials, (α-AlOOH or γ-Al(2)O(3)), were mixed with alginate embedded with yeast; and finally these carriers were cross-linked through the double oxirane. The alginate-based immobilized yeast with MCM-41 exhibited much shorter fermentation time and higher ethanol concentration than pure alginate and other composite carriers with the highest cell concentration of 4.8×10(9) cells/mL. The composite carrier maintains the highest ethanol productivity of 6.55 g/L/h for 60 days in continuous fermentation process, implying good operational durability for commercial applications. The reason for the higher bio-catalytical function of the immobilized yeast might lay in the uniformly yeast distribution in the bio-reactor and high yeast cell concentration, which contributed by the improved transmission of fermentation media and combined effects of yeast adsorption by MCM-41 and embedment by alginate.
authors.affiliation: State Key Laboratory of Hollow-fiber Membrane Materials and Membrane Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Chunming
authors.initials: C
authors.lastname: Zheng
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Landong
authors.initials: L
authors.lastname: Li
authors.forename: Naijia
authors.initials: N
authors.lastname: Guan
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D007700:Kinetics; D008965:Molasses / Q000032:analysis*; D010865:Pilot Projects; D016062:Porosity / Q000187:drug effects; D059027:Recycling; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology*; D017641:Zeolites / Q000494:pharmacology*
chemical_list: D000464:Alginates; D002241:Carbohydrates; D006603:Hexuronic Acids; C509968:MCM-41; D017641:Zeolites; D000431:Ethanol; D012822:Silicon Dioxide; D020723:Glucuronic Acid