title: Autoproteolytic fragments are intermediates in the oligomerization/aggregation of the Parkinson's disease protein alpha-synuclein as revealed by ion mobility mass spectrometry.
abstract: Gas-phase protein separation by ion mobility: With its ability to separate the Parkinson's disease protein α-synuclein and its autoproteolytic products-despite the small concentrations of the latter-ion-mobility MS has enabled the characterization of intermediate fragments in in vitro oligomerization-aggregation. In particular, a possible key fragment, the highly aggregating C-terminal fragment, αSyn(72-140), has been revealed.
authors.affiliation: Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Camelia
authors.initials: C
authors.lastname: Vlad
authors.forename: Kathrin
authors.initials: K
authors.lastname: Lindner
authors.forename: Christiaan
authors.initials: C
authors.lastname: Karreman
authors.forename: Stefan
authors.initials: S
authors.lastname: Schildknecht
authors.forename: Marcel
authors.initials: M
authors.lastname: Leist
authors.forename: Nick
authors.initials: N
authors.lastname: Tomczyk
authors.forename: John
authors.initials: J
authors.lastname: Rontree
authors.forename: James
authors.initials: J
authors.lastname: Langridge
authors.forename: Karin
authors.initials: K
authors.lastname: Danzer
authors.forename: Thomas
authors.initials: T
authors.lastname: Ciossek
authors.forename: Alina
authors.initials: A
authors.lastname: Petre
authors.forename: Michael L
authors.initials: ML
authors.lastname: Gross
authors.forename: Bastian
authors.initials: B
authors.lastname: Hengerer
authors.forename: Michael
authors.initials: M
authors.lastname: Przybylski
mesh_terms: D001704:Biopolymers / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010300:Parkinson Disease / Q000378:metabolism*; D059748:Proteolysis; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D001704:Biopolymers; D051844:alpha-Synuclein