title: Anticoagulation of diethyl citrate and its comparison with sodium citrate in an animal model.
abstract: OBJECTIVE
  To improve the side effects caused by sodium citrate (Na(3)Cit), the anticoagulant effects of diethyl citrate (Et(2)Cit) were investigated.
  
  
  METHODS
  The in vitro anticoagulant effects and dissociation capacity of the chelate of Et(2)Cit with calcium ions were compared with those of Na(3)Cit in rabbits.
  
  
  RESULTS
  The activated coagulation time test showed that blood clotting time exceeded 1,200 s when the concentrations of Et(2)Cit and Na(3)Cit were greater than 87.2 and 8.72 mmol/l, respectively. The concentrations of free calcium ions in blood c(Ca(2+)) were reduced when Et(2)Cit was injected into the rabbits.
  
  
  CONCLUSIONS
  Et(2)Cit reduces the concentration of ionized Ca(2+) in blood and has anticoagulant effects. The dissociation of the chelate of Et(2)Cit with Ca(2+) was faster than that of Na(3)Cit with Ca(2+) within 10 min after injection. The recovery speed of blood calcium concentration with Et(2)Cit was more rapid than that with Na(3)Cit. The findings show that Et(2)Cit prevents hypocalcemia.
authors.affiliation: Department of Nephrology, the Second Hospital of Xi'an Jiaotong University, Xi'an, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Xiu-Qiong
authors.initials: XQ
authors.lastname: Yao
authors.forename: Bao-Song
authors.initials: BS
authors.lastname: Gui
authors.forename: Yan
authors.initials: Y
authors.lastname: Ou
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology / Q000627:therapeutic use*; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D002118:Calcium / Q000097:blood*; D002614:Chelating Agents / Q000494:pharmacology / Q000627:therapeutic use; D002951:Citrates / Q000494:pharmacology / Q000627:therapeutic use*; D006996:Hypocalcemia / Q000517:prevention & control*; D008297:Male; D011817:Rabbits; D000077559:Sodium Citrate
chemical_list: D000925:Anticoagulants; D002614:Chelating Agents; D002951:Citrates; D000077559:Sodium Citrate; C574095:diethyl citrate; D002118:Calcium