title: Impact of interleukin-8 gene polymorphisms and environmental factors on oral cancer susceptibility in Taiwan.
abstract: OBJECTIVE
  Interleukin-8 (IL-8), which is an angiogenic chemokine with a high expression level in tumor tissues, plays important roles in developing many human malignancies including oral squamous cell carcinoma (OSCC). This study was designed to examine the association of IL-8 gene polymorphisms with the susceptibility and clinicopathological characteristics of OSCC.
  
  
  METHODS
  A total of 270 patients with OSCC and 350 healthy control subjects were recruited. Four single nucleotide polymorphisms (SNPs) of IL-8 genes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping analysis.
  
  
  RESULTS
  Results showed that four IL-8 SNPs (-251 T/A, +781 C/T, +1633 C/T, and +2767 A/T) were not associated with oral cancer susceptibility as well as clinicopathological parameters. But among 345 smokers, IL-8 polymorphisms carriers with betel quid chewing were found to have a 17.41- to 23.14-fold risk to have oral cancer compared to IL-8 wild-type carriers without betel quid chewing. Among 262 betel quid chewers, IL-8 polymorphisms carriers with smoking have a 10.54- to 20.44-fold risk to have oral cancer compared to those who carried wild type without smoking.
  
  
  CONCLUSIONS
  Our results suggest that the combination of IL-8 gene polymorphisms and environmental carcinogens might be highly related to the risk of oral cancer.
authors.affiliation: School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: C-M
authors.initials: CM
authors.lastname: Liu
authors.forename: C-J
authors.initials: CJ
authors.lastname: Yeh
authors.forename: C-C
authors.initials: CC
authors.lastname: Yu
authors.forename: M-Y
authors.initials: MY
authors.lastname: Chou
authors.forename: C-H
authors.initials: CH
authors.lastname: Lin
authors.forename: L-H
authors.initials: LH
authors.lastname: Wei
authors.forename: C-W
authors.initials: CW
authors.lastname: Lin
authors.forename: S-F
authors.initials: SF
authors.lastname: Yang
authors.forename: M-H
authors.initials: MH
authors.lastname: Chien
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000225:Adenine; D001114:Areca / Q000009:adverse effects; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D016022:Case-Control Studies; D003596:Cytosine; D005260:Female; D059647:Gene-Environment Interaction; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D013941:Thymine
chemical_list: D020413:3' Untranslated Regions; C508606:CXCL8 protein, human; D002273:Carcinogens; D016209:Interleukin-8; D003596:Cytosine; D000225:Adenine; D013941:Thymine