title: Host factors associated with the kinetics of Epstein-Barr virus DNA load in patients with primary Epstein-Barr virus infection.
abstract: The aims of this study were to elucidate the kinetics of Epstein-Barr virus (EBV) DNA load in serially collected peripheral blood mononuclear cells of patients with primary EBV infection, and to determine the correlated host factors. Blood samples were collected from 24 patients with primary EBV infection. EBV DNA copy numbers were measured using real-time polymerase chain reaction. Based on the kinetics of EBV DNA load, the 24 patients were divided into two groups: rapid regression and slow regression. Eighteen of the 24 patients (75%) were included in the slow regression and 6 (25%) in the rapid regression group. No statistically significant differences were observed between the two groups in clinical features and laboratory findings. However, acute phase (3 to 10 days after the onset of the illness) serum samples from six children in the slow regression and four in the rapid regression group revealed significantly higher serum interleukin (IL)-1β (P= 0.018), IL-12 (P= 0.009), tumor necrosis factor-α (P= 0.019), interferon-inducible protein 10, and monokine induced by interferon γ concentrations in the rapid regression than the slow regression group. On the other hand, sera from six children in the slow regression and four in the rapid regression group in the convalescent phase (14 to 21 days after the onset of the illness) showed no statistically significant differences between the two groups in these biomarker concentrations. Based on this, it was concluded that the kinetics of EBV DNA load can be divided to two different patterns after primary EBV infection, and immune response might be associated with viral clearance.
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Nakai
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Ken
authors.initials: K
authors.lastname: Sugata
authors.forename: Hiroko
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Enomoto
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Asano
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohashi
authors.forename: Tomochika
authors.initials: T
authors.lastname: Kato
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000293:Adolescent; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000378:metabolism* / Q000821:virology; D005260:Female; D018628:Gene Dosage; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007223:Infant; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D008297:Male; D019562:Viral Load
chemical_list: D018925:Chemokines; D016207:Cytokines; D004279:DNA, Viral