title: The influence of different submerged cultivation conditions on mycelial biomass and protease production by Lentinus citrinus Walleyn et Rammeloo DPUA 1535 (Agaricomycetideae).
abstract: The influence of different carbon and nitrogen sources, pH of the culture medium, and temperature and period of cultivation on mycelial biomass production and protease activity by Lentinus citrinus DPUA 1535 were investigated in submerged culture. A 2(5) full factorial design with three central points was employed, and the results showed that at a significance level of 95% only nitrogen source and temperature were statistically significant for mycelial biomass production. On the other hand, for protease activity all factors and some interactions were significant, and the temperature and nitrogen source had the most significant effect. The best condition for mycelial biomass production (5.76 mg mL(-1)) and protease activity (32.3 U mL(-1)) was obtained in medium formulated with 0.5% soluble starch, 0.2% gelatin, pH 7.0, 25 degrees C, in 5 days.
authors.affiliation: Culture Collection DPUA/UFAM, Federal University of Amazon, Manaus, AM, Brazil. l_kirsch@hotmail.com
authors.forename: Larissa de Souza
authors.initials: Lde S
authors.lastname: Kirsch
authors.forename: Ana Carolina dos Santos
authors.initials: AC
authors.lastname: Pinto
authors.forename: Tatiana Souza
authors.initials: TS
authors.lastname: Porto
authors.forename: Ana Lúcia Figueiredo
authors.initials: AL
authors.lastname: Porto
authors.forename: Maria Francisca Simas
authors.initials: MF
authors.lastname: Teixeira
mesh_terms: D018533:Biomass; D002244:Carbon; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D020074:Lentinula / Q000201:enzymology* / Q000254:growth & development* / Q000502:physiology; D025282:Mycelium / Q000254:growth & development*; D009584:Nitrogen; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D011134:Polysaccharides / Q000096:biosynthesis; D013696:Temperature
chemical_list: D003470:Culture Media; D011134:Polysaccharides; D002244:Carbon; D010447:Peptide Hydrolases; D009584:Nitrogen