title: PAX8 is expressed in the majority of renal epithelial neoplasms: an immunohistochemical study of 223 cases using a mouse monoclonal antibody.
abstract: OBJECTIVE
  PAX8 is a cell lineage-specific transcription factor which plays a crucial role in the organogenesis of the kidney, thyroid gland and Müllerian duct. A previous study showed that PAX8 is a specific and sensitive marker for both renal and ovarian carcinomas. The purpose of this study is to investigate PAX8 expression using a new monoclonal PAX8 antibody in a larger number of renal epithelial neoplasms including clear cell renal cell carcinoma, papillary renal cell carcinoma, chromophobe renal cell carcinoma and renal oncocytoma.
  
  
  METHODS
  PAX8 immunohistochemical staining was performed on tissue microarrays containing 84 cases of clear cell renal cell carcinoma, 66 cases of chromophobe renal cell carcinoma, 57 cases of papillary renal cell carcinoma and 16 cases of renal oncocytoma.
  
  
  RESULTS
  PAX8 expression was detected in 93% (78/84) of cases of clear cell renal cell carcinoma, 80% (53/66) of cases of chromophobe renal cell carcinoma, 95% (54/57) of cases of papillary renal cell carcinoma and 94% (15/16) of cases of renal oncocytoma.
  
  
  CONCLUSIONS
  PAX8 is expressed in the majority of renal epithelial neoplasms including renal cell carcinomas and oncocytomas and the monoclonal PAX8 antibody is more sensitive than polyclonal antibody to detect chromophobe renal cell carcinoma. These results showed that PAX8 is a valuable marker for nephric neoplasms.
authors.affiliation: Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA.
authors.forename: Yingchuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Arndt
authors.initials: A
authors.lastname: Hartmann
authors.forename: Christine
authors.initials: C
authors.lastname: Stoehr
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zhang
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Tacha
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Montironi
authors.forename: Antonio
authors.initials: A
authors.lastname: Lopez-Beltran
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
mesh_terms: D018249:Adenoma, Oxyphilic / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D001706:Biopsy; D002291:Carcinoma, Papillary / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D004847:Epithelial Cells / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D051379:Mice; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis* / Q000276:immunology; D011237:Predictive Value of Tests; D046888:Tissue Array Analysis
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D051761:Paired Box Transcription Factors