title: Inhibitory activity of α-amylase and α-glucosidase by plant extracts from the Brazilian cerrado.
abstract: Diabetes mellitus is the most common disease in the world. One therapeutic approach for treating diabetes is inhibition of α-amylase and α-glucosidase activities to reduce postprandial blood glucose levels. In vitro tests showed that several plant extracts from Brazilian cerrado species can inhibit the activity of α-amylase and α-glucosidase. The extracts of Eugenia dysenterica, Stryphnodendron adstringens, Pouteria caimito, Pouteria ramiflora, and Pouteria torta showed strong α-amylase and α-glucosidase inhibitory activity. Eugenia dysenterica, P. caimito, P. ramiflora, and P. torta aqueous extracts exerted the highest activity against α-amylase (IC₅₀) values of 14.93, 13.6, 7.08, and 5.67 µg/mL, respectively) and α-glucosidase (IC₅₀ values of 0.46, 2.58, 0.35, and 0.22 µg/mL, respectively). Stryphnodendron adstringens ethanol extract also exhibited inhibitory activity against both enzymes (IC₅₀) 1.86 µg/mL against α-amylase and 0.61 µg/mL against α-glucosidase). The results suggest that the activity of these cerrado plants on α-amylase and α-glucosidase represents a potential tool for development of new strategies for treatment of diabetes.
authors.affiliation: Department of Pharmaceutical Sciences, School of Health Sciences, Campus Darcy Ribeiro, University of Brasília, Brasília, Brazil.
authors.forename: Paula Monteiro de
authors.initials: PM
authors.lastname: Souza
authors.forename: Paloma Michelle de
authors.initials: PM
authors.lastname: Sales
authors.forename: Luiz Alberto
authors.initials: LA
authors.lastname: Simeoni
authors.forename: Elton Clementino
authors.initials: EC
authors.lastname: Silva
authors.forename: Dâmaris
authors.initials: D
authors.lastname: Silveira
authors.forename: Pérola de Oliveira
authors.initials: Pde O
authors.lastname: Magalhães
mesh_terms: D001938:Brazil; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D032132:Pouteria / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases