title: Development and certification of a standard reference material for vitamin D metabolites in human serum.
abstract: The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health's Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D(2), 25(OH)D(3), and 3-epi-25(OH)D(3). Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D(3) proved to be essential for accurate determination of the metabolites.
authors.affiliation: Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States. karen.phinney@nist.gov
authors.forename: Karen W
authors.initials: KW
authors.lastname: Phinney
authors.forename: Mary
authors.initials: M
authors.lastname: Bedner
authors.forename: Susan S-C
authors.initials: SS
authors.lastname: Tai
authors.forename: Veronica V
authors.initials: VV
authors.lastname: Vamathevan
authors.forename: Lane C
authors.initials: LC
authors.lastname: Sander
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Sharpless
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Wise
authors.forename: James H
authors.initials: JH
authors.lastname: Yen
authors.forename: Rosemary L
authors.initials: RL
authors.lastname: Schleicher
authors.forename: Madhulika
authors.initials: M
authors.lastname: Chaudhary-Webb
authors.forename: Christine M
authors.initials: CM
authors.lastname: Pfeiffer
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Betz
authors.forename: Paul M
authors.initials: PM
authors.lastname: Coates
authors.forename: Mary Frances
authors.initials: MF
authors.lastname: Picciano
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000592:standards
chemical_list: D014807:Vitamin D; C104450:25-hydroxyvitamin D