title: Selection of recombinant MVA by rescue of the essential D4R gene.
abstract: Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.
authors.affiliation: Baxter BioScience, Biomedical Research Center, Uferstrasse 15, 2304 Orth an der Donau, Austria.
authors.forename: Patricia S
authors.initials: PS
authors.lastname: Ricci
authors.forename: Birgit
authors.initials: B
authors.lastname: Schäfer
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Kreil
authors.forename: Falko G
authors.initials: FG
authors.lastname: Falkner
authors.forename: Georg W
authors.initials: GW
authors.lastname: Holzer
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004261:DNA Replication; D020043:Genes, Essential; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D011995:Recombination, Genetic; D017384:Sequence Deletion; D051981:Uracil-DNA Glycosidase / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D051981:Uracil-DNA Glycosidase