title: Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development.
abstract: We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).
authors.affiliation: Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, Singapore, Singapore.
authors.forename: Deepak
authors.initials: D
authors.lastname: Choudhury
authors.forename: Danny
authors.initials: D
authors.lastname: van Noort
authors.forename: Ciprian
authors.initials: C
authors.lastname: Iliescu
authors.forename: Baixue
authors.initials: B
authors.lastname: Zheng
authors.forename: Kar-Lai
authors.initials: KL
authors.lastname: Poon
authors.forename: Svetlana
authors.initials: S
authors.lastname: Korzh
authors.forename: Vladimir
authors.initials: V
authors.lastname: Korzh
authors.forename: Hanry
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010477:Perfusion; D015203:Reproducibility of Results; D014635:Valproic Acid / Q000494:pharmacology*; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development*
chemical_list: D014635:Valproic Acid