title: Acute toxicity of manganese in goldfish Carassius auratus is associated with oxidative stress and organ specific antioxidant responses.
abstract: Manganese is a relatively common, yet poorly studied element in freshwater ecosystems, where it can be significantly bioconcentrated. The knowledge about the mechanisms of Mn toxicity on fish health is still limited. The aim of the present study was to assess the potential induction of oxidative stress and the antioxidant response after a 96 h waterborne Mn-exposure (at 0.1 and 1mM) in gill, kidney, liver and brain of goldfish (Carassius auratus). Mn 1mM induced an increase of lipid hydroperoxides, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in all tissues with the exception of SOD inhibition in the brain. Particular response of catalase (CAT) was indicated-its inhibition in the liver and kidney, but activation in the gill. Exposure to Mn 0.1mM provoked most prominent changes in the liver and did not change the indexes in brain. These results strongly suggest that Mn exposure caused a generalized oxidative stress in the fish and revealed an organ specific antioxidant response involving a differential modulation of the SOD, CAT and GPx activities.
authors.affiliation: Department of Environmental Biology and Public Health, Faculty of Experimental Sciences, University of Huelva, Avda. Fuerzas Armadas s/n, 21071 Huelva, Spain. mcvlacerda@terra.com.br
authors.forename: Maria Conceiçao
authors.initials: MC
authors.lastname: Vieira
authors.forename: Rafael
authors.initials: R
authors.lastname: Torronteras
authors.forename: Francisco
authors.initials: F
authors.lastname: Córdoba
authors.forename: Antonio
authors.initials: A
authors.lastname: Canalejo
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D006054:Goldfish / Q000502:physiology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008345:Manganese / Q000378:metabolism / Q000633:toxicity*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D008054:Lipid Peroxides; D014874:Water Pollutants, Chemical; D008345:Manganese; D002374:Catalase; D013482:Superoxide Dismutase