title: A coordination complex system for generic, ultrafast, and sensitive multimode fluorescent staining of biomolecules.
abstract: Gel electrophoresis staining methodologies documented thus far are largely utilized in a biomolecule context-dependent manner. We report herein the development of a generic, ultrafast, and sensitive multimode fluorescent system for the efficient identification of DNA, RNA, and proteins. Interaction between a positively charged, planar ligand-based coordination complex with partner biomolecule leads to aggregation-induced fluorescence quenching and allows for the image contrast generation within one minute. Alternatively, successive reactions of the biomolecule-loaded gel with cation and ligand, in either order of sequence, provide an equally effective staining efficacy. Image contrast reversal is accomplished through a facile washing or photobleaching procedure. The versatility in the applicable target species and signal generation modes provides a hint at the design of novel staining structures and potentially enables the high-throughput readout of biomolecules.
authors.affiliation: Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
authors.forename: Xingqiang
authors.initials: X
authors.lastname: Liu
authors.forename: Lingjuan
authors.initials: L
authors.lastname: Li
authors.forename: Jingjing
authors.initials: J
authors.lastname: Sun
authors.forename: Yishu
authors.initials: Y
authors.lastname: Yan
authors.forename: Xin
authors.initials: X
authors.lastname: Shu
authors.forename: Baoqing
authors.initials: B
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Sha
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.forename: Sha
authors.initials: S
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012313:RNA; D004247:DNA