title: Expression of ESBL-like activity in infrequently encountered members of the family Enterobacteriaceae.
abstract: A collection of 94 unusual members of the Enterobacteriaceae were screened for the presence of extended spectrum β-lactamases (ESBLs) using the MicroScan ESβL plus dried confirmation panel. Presumptively positive strains were then confirmed for the presence of an ESBL by double disk diffusion, E-test strips (AB Biodisk, Solna, Sweden) and PCR for SHV, TEM, and CTX-M2 genes. Of the 18 strains initially positive on the ESβL panel only three strains (Leminorella grimontii, Klebsiella ozaenae, and Kluyvera ascorbata) were positive by confirmation methods. These results suggest laboratories should be cautious regarding the methodology employed in screening for the presence of ESBLs in enteric bacteria. However, it should be noted that of the 94 strains, 29 were found to be resistant to two or more of the antibiotics present in the MicroScan ESβL plus panel indicating that there are potential treatment issues with these organisms despite their lack of ESBLs.
authors.affiliation: Microbial Diseases Laboratory, CA State Department of Public Health, Richmond, CA, USA. Sharon.Abbott@cdph.ca.gov
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Abbott
authors.forename: Janice A
authors.initials: JA
authors.lastname: Lidgard
authors.forename: Wendy K W
authors.initials: WK
authors.lastname: Cheung
authors.forename: Martha N
authors.initials: MN
authors.lastname: Obeso
authors.forename: Zenda L
authors.initials: ZL
authors.lastname: Berrada
authors.forename: J Michael
authors.initials: JM
authors.lastname: Janda
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D015870:Gene Expression; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases