title: [Dependence of protective effect of alpha-tocopherol on its concentration and time of action on PC12 cell under conditions of oxidative stress].
abstract: At the short-term incubation (0.5 and 1.5 h) of cells of the PC12 neuronal line with alpha-tocopherol, its protective effect against the cytotoxic hydrogen peroxide action was increased with rise of its concentration in samples; the protection was practically absent at action of nanomolar antioxidant concentrations, but was well expressed at its micromolar concentrations. These data agree with the concept that alpha-tocopherol increases the cell living activity by reacting directly with free radicals, which leads to formation of the less reactive compounds deprived of non-paired electron. The evidence is obtained that at the long-term action on PC12 cells, alpha-tocopherol not only in micro-, but also in nanomolar concentrations increases statistically significantly the cell living activity under conditions of oxidative stress. As follows from the obtained data, an important role in realization of the alpha-tocopherol protective effect at the long-term incubation with it seems to be played by modulation by this antioxidant of activity of protein kinase activated by extracellular signaling, phosphatidylinosite 3-kinase, and protein kinase C.
authors.forename: T V
authors.initials: TV
authors.lastname: Sokolova
authors.forename: M I
authors.initials: MI
authors.lastname: Rychkova
authors.forename: I V
authors.initials: IV
authors.lastname: Voĭnova
authors.forename: N F
authors.initials: NF
authors.lastname: Avrova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D006836:Hydro-Lyases / Q000032:analysis; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D020011:Protective Agents / Q000378:metabolism* / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D024502:alpha-Tocopherol / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D006861:Hydrogen Peroxide; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases; D006836:Hydro-Lyases; C045581:lactate dehydratase; D024502:alpha-Tocopherol