title: Designing bifunctionalized gold nanoparticle for colorimetric detection of Pb2+ under physiological condition.
abstract: In the present work, a kind of peptide functionalized gold nanoparticle (AuNP) has been synthesized and employed for colorimetric detection of Pb(2+) in both aqueous solution and living cell. The AuNPs are capped by two peptide ligands: glutathione (GSH) and pentapeptide (CALNN). The GSH is used as a functional group for selectively sensing Pb(2+) by coordination reaction, and CALNN is employed as a stabilize ligand for improving the stability of AuNPs under physiological condition, respectively. The AuNP enables to strongly interact with Pb(2+) that leads to distinct color change of solution. Under the optimized molar ratio of GSH to CALNN on the AuNP surface, the colorimetric assay for detecting Pb(2+) in living cell downs to 2.9 fmol Pb(2+)per cell (3 times of standard deviation, 3σ) with linear relationship from 2.9 to 37.7 fmol Pb(2+) per cell. In addition, the method also shows highly selective detection toward Pb(2+) against other common metal ions in both aqueous solution and living cell.
authors.affiliation: Department of Medicinal Chemistry and Pharmaceutical analysis, Guangdong Medical College, Dongwan, Guangdong, PR China.
authors.forename: Derong
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Jine
authors.initials: J
authors.lastname: Wang
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007854:Lead / Q000032:analysis* / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D007854:Lead; D006046:Gold