title: Effect of interleukin-6 (IL-6) on the vascular smooth muscle contraction in abdominal aorta of rats with streptozotocin-induced diabetes.
abstract: Patients with type 1 diabetes are at a risk of hypertension. However, the mechanisms behind the findings are not completely known. The aim of the present study was to investigate involvement of interleukin-6 (IL-6) on the contraction of abdominal aorta in rats with type 1 diabetes. IL-6 levels in the plasma of rats with streptozotocin (STZ)-induced diabetes were determined by ELISA. The abdominal aorta was dissected free of fat and connective tissues and then cut into spiral rings. The endothelium-denuded strip was vertically suspended in tissue chambers containing 5 ml Krebs solution at 37 degrees C and bubbled continuously with 95% O2-5% CO2. The effects of phenylephrine (Phe) on the contractile responses of abdominal aorta were recorded. The effects of IL-6 and anti-rat IL-6 antibody on the Phe-induced response were also examined. Plasma levels of IL-6 increased time-dependently in rats with STZ-induced diabetes. Phe caused concentration-dependent contraction in aortic rings. Phe-induced contractions were higher in vascular strips of STZ-induced diabetic rats than that of control rats. Pretreatment of vascular strips with IL-6 for 1 h did not cause contraction but enhanced the contraction in response to Phe. Treatment of the vascular strips with an anti-IL-6 antibody for 1 h decreased the Phe-induced contractions. These results suggest that IL-6 causes vascular smooth muscle contraction in abdominal aorta of rats with type 1 diabetes.
authors.affiliation: Institute of Physiology, School of Medicine, Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Tang
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhou
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Shan
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Yang
authors.forename: Xin-Wen
authors.initials: XW
authors.lastname: Chang
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.forename: Paulus S
authors.initials: PS
authors.lastname: Wang
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Xie
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D015850:Interleukin-6 / Q000502:physiology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology*; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D015850:Interleukin-6; D010656:Phenylephrine; D013311:Streptozocin