title: [Revealing the genetic determinants of Pks-pathogenicity island in clinical strains of enterobacteria].
abstract: OBJECTIVE
  Detection by PCR the frequency of clbB, clbN, clbA H clbQ genes of Pks-pathogenicity island in clinical strains ofenterobacteria.
  
  
  METHODS
  112 strains various genera and species of enterobacteria, including 16 museum and 96 clinical are investigated. Isolated strains represents Escherichia species (n = 68), Klebsiella (n = 16), Enterobacter (n = 9), Serratia (n = 7) and others minor species of Enterobacteriaceae family (n = 12). Fifty nine strains isolated from urine of urinary tract infection, 26 isolates from intestines of patients with dysbiosis and 11--from children with complications after a liver transplantation. A total bacterial isolates were screened by multiplex PCRforthe presence ofclbB, clbN, clbA and clbQ genes.
  
  
  RESULTS
  Among 41 uropathogenic E.coli it is revealed 15 (36,6%) Pks-positive strains carring all of clbB, clbN, clbA ? clbQ genes, that composed 27,1% from total number of the enterobacteria, isolates from urine. Among 44 clinical isolates of various species of enterobacteria only one Pks-positive strain K. pneumoniae was revealed. Strains enterobacteria, isolated at pyoinflammatory complications after liver transplantation (n = 11) and isolates from intestinal tract in dysbiosis (n = 26), were Pks-negative.
  
  
  CONCLUSIONS
  The clbB, clbN, clbA ? clbQ genes of the Pks-island which have been detected in 36,6 % E. coli urological strains are markers of pathogenicity of clinical isolates of extraintestinal origin and advisable of their detection by PCR.
authors.forename: S V
authors.initials: SV
authors.lastname: Fialkina
authors.forename: V M
authors.initials: VM
authors.lastname: Bondarenko
authors.forename: Iu L
authors.initials: IuL
authors.lastname: Naboka
authors.forename: E A
authors.initials: EA
authors.lastname: Miroshnichenko
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004754:Enterobacter / Q000235:genetics / Q000472:pathogenicity; D004755:Enterobacteriaceae / Q000235:genetics* / Q000472:pathogenicity*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D044404:Genomic Islands / Q000235:genetics*; D006801:Humans; D007709:Klebsiella / Q000235:genetics / Q000472:pathogenicity; D048630:Polyketide Synthases / Q000037:antagonists & inhibitors / Q000235:genetics; D012705:Serratia / Q000235:genetics / Q000472:pathogenicity; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D048630:Polyketide Synthases