title: MicroRNA expression analysis using the Affymetrix Platform.
abstract: Microarrays have been used extensively for messenger RNA expression monitoring. Recently, microarrays have been designed to interrogate expression levels of noncoding RNAs. Here, we describe methods for RNA labeling and the use of a miRNA array to identify and measure microRNA present in RNA samples.
authors.affiliation: Affymetrix, Santa Clara, CA, USA. suzanne_dee@affymetrix.com
authors.forename: Suzanne
authors.initials: S
authors.lastname: Dee
authors.forename: Robert C
authors.initials: RC
authors.lastname: Getts
mesh_terms: D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D011061:Poly A; D011786:Quality Control; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D035683:MicroRNAs; D011061:Poly A; D012313:RNA