title: Development of sensor cells using NF-κB pathway activation for detection of nanoparticle-induced inflammation.
abstract: The increasing use of nanomaterials in consumer and industrial products has aroused concerns regarding their fate in biological systems. An effective detection method to evaluate the safety of bio-nanomaterials is therefore very important. Titanium dioxide (TiO(2)), which is manufactured worldwide in large quantities for use in a wide range of applications, including pigment and cosmetic manufacturing, was once thought to be an inert material, but recently, more and more studies have indicated that TiO(2) nanoparticles (TiO(2) NPs) can cause inflammation and be harmful to humans by causing lung and brain problems. In order to evaluate the safety of TiO(2) NPs for the environment and for humans, sensor cells for inflammation detection were developed, and these were transfected with the Toll-like receptor 4 (TLR4) gene and Nuclear Factor Kappa B (NF-κB) reporter gene. NF-κB as a primary cause of inflammation has received a lot of attention, and it can be activated by a wide variety of external stimuli. Our data show that TiO(2) NPs-induced inflammation can be detected by our sensor cells through NF-κB pathway activation. This may lead to our sensor cells being used for bio-nanomaterial safety evaluation.
authors.affiliation: Cell-Materials Interaction Group, Biomaterials Unit, Nano-Bio Field, National Institute for Materials Science, 1-1, Namiki, Tsukuba, Ibaraki, 305-0044, Japan. chen.peng@nims.go.jp
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Satoshi
authors.initials: S
authors.lastname: Migita
authors.forename: Koki
authors.initials: K
authors.lastname: Kanehira
authors.forename: Shuji
authors.initials: S
authors.lastname: Sonezaki
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Taniguchi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017930:Genes, Reporter; D006801:Humans; D007249:Inflammation / Q000139:chemically induced* / Q000175:diagnosis* / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D053758:Nanoparticles / Q000009:adverse effects*; D010957:Plasmids / Q000235:genetics; D014025:Titanium / Q000009:adverse effects; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014162:Transfection
chemical_list: D016328:NF-kappa B; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; C009495:titanium dioxide; D014025:Titanium