title: [RNA interference of proteasome subunit of PSMbeta7 gene restricts proteasome subunit PSMbeta1 and PSMbeta5 mRNA expression and peptidyl-glutamyl peptide-hydrolyzing proteasome activity in neonatal cardiomyocytes].
abstract: Using small interfering RNA (siRNA) transfection of neonatal cardiomyocytes to inhibit expression of nonproteolytic proteasome beta7 subunit, we observed a significant decrease in beta1 proteolytic subunit mRNA expression. Proteasome peptidyl-glutamyl peptide-hydrolyzing activity decreased to 28% (0.48 +/- 0.2 nM AMC/min) compared to control (1.7 +/- 0.5 nM AMC/min) (P < 0.05). Beta5 Subunit mRNA expression decreased 21 times (P < 0.05) with no changes in its chymotrypsin-like activity. Proteasome trypsin-like activity and activity of another proteolytic enzyme tripeptidyl-peptidase II remained unchanged.
authors.forename: V O
authors.initials: VO
authors.lastname: Kyrychenko
authors.forename: D O
authors.initials: DO
authors.lastname: Pashevin
authors.forename: L V
authors.initials: LV
authors.lastname: Tumanovs'ka
authors.forename: V Ie
authors.initials: VIe
authors.lastname: Dosenko
authors.forename: O O
authors.initials: OO
authors.lastname: Moĭbenko
mesh_terms: D000626:Aminopeptidases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002918:Chymotrypsin / Q000737:chemistry; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D061251:Primary Cell Culture; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D061988:Proteasome Inhibitors; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis*; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D061988:Proteasome Inhibitors; D021122:Protein Subunits; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C048001:tripeptidyl-peptidase 2; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D046988:Proteasome Endopeptidase Complex