title: Proteomic analysis of tumor tissue in CT-26 implanted BALB/C mouse after treatment with ascorbic acid.
abstract: Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression and protein modification. Proteome changes of tumor tissue were investigated after intraperitoneal administration of a high concentration of ascorbic acid in BALB/C mice implanted with CT-26 cancer cells using two-dimensional gel electrophoresis and mass spectrometry. Eighteen protein spots were identified whose expression was different between control and ascorbic acid treatment groups. In particular, eukaryotic translation initiation factor 3 subunit 1, nucleophosmin, latexin, actin-related protein 2/3 complex subunit 5, M2-type pyruvate kinase, vimentin, tumor protein translationally-controlled 1, RAS oncogene family Ran, plastin 3 precursor, ATPase, Rho GDT dissociation inhibitor β, and proteasome activator subunit 2 expression were quantitatively up-regulated. The increase in the level of these proteins was accompanied by an increase in mRNA level. The cytoskeleton protein actin, vimentin, and tumor protein translationally-controlled 1 showed quantitative expression profile differences. A change in actin cytoskeleton distribution, functionally relevant to the proteome result, was observed after treatment with ascorbic acid. These results suggest a previously undefined role of ascorbic acid in the regulation of cytoskeleton remodeling in tumor tissues.
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Sungbuk-ku, Seoul, Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Lee
authors.forename: Gunsup
authors.initials: G
authors.lastname: Lee
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Yeom
authors.forename: Byungjo
authors.initials: B
authors.lastname: Na
authors.forename: Seyeon
authors.initials: S
authors.lastname: Park
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome; D012333:RNA, Messenger; D001205:Ascorbic Acid