title: Galangin induces apoptosis in gastric cancer cells via regulation of ubiquitin carboxy-terminal hydrolase isozyme L1 and glutathione S-transferase P.
abstract: Galangin has been shown to have anti-cancer property against several types of cancer cells. Many studies have described the anti-oxidant and apoptotic effects of galangin. However, the mechanism of galangin-induced apoptosis has not yet been studied for human gastric cancer cells. We investigated galangin-induced apoptosis of human gastric cancer SNU-484 cells. Galangin inhibited proliferation of SNU-484 cells in a dose- and time-dependent manner. The results showed that galangin significantly decreased the viability of SNU-484 cells at 50-200 μM for 24 h and 48 h. Galangin-induced cell death was characterized with the changes in cell morphology, DNA fragmentation, cell cycle, activation of caspase-3/-9, poly (ADP-ribose) polymerase (PARP) cleavage, and expression of MAP kinase such as ERK1/2 and JNK. For identification of proteins potentially involved in apoptosis, a two-dimensional electrophoresis was employed. Proteomic analysis showed that several proteins were associated with anti-cancer properties of galangin. Of particular interest, these proteins included ubiquitin carboxy-terminal hydrolase isozyme L1 (Uch-L1) and glutathione S-transferase P (GSTP), which are involved in apoptosis of SNU-484 cells. Western blot analysis confirmed up-regulation of Uch-L1 and down-regulation of GSTP following galangin treatment. Our results suggest that Uch-L1 and GSTP be involved in galangin-induced apoptosis in human gastric cancer SNU-484 cells.
authors.affiliation: Department of Biological Science, Gachon University of Medicine and Science, 534-2 Yeonsu-dong, Yeonsu-gu, Incheon 406-799, Republic of Korea.
authors.forename: Deuk Ae
authors.initials: DA
authors.lastname: Kim
authors.forename: Young Keul
authors.initials: YK
authors.lastname: Jeon
authors.forename: Myeong Jin
authors.initials: MJ
authors.lastname: Nam
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D020552:Comet Assay; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation; D005419:Flavonoids / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*; D043222:Ubiquitin Thiolesterase / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D007527:Isoenzymes; C037032:galangin; D005982:Glutathione Transferase; D043222:Ubiquitin Thiolesterase