title: Quantitative determination of pregnanes from aerial parts of Caralluma species using HPLC-UV and identification by LC-ESI-TOF.
abstract: An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1-5 and 3-15 microg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LCIMS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.
authors.affiliation: The University of Mississippi, School of Pharmacy, National Center for Natural Products Research, University, MS 38677, USA.
authors.forename: Bharathi
authors.initials: B
authors.lastname: Avula
authors.forename: Yatin J
authors.initials: YJ
authors.lastname: Shukla
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D029062:Apocynaceae / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D019587:Dietary Supplements / Q000032:analysis; D008872:Microwaves; D029747:Opuntia / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis; D011278:Pregnanes / Q000032:analysis*; D012044:Regression Analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics
chemical_list: D010936:Plant Extracts; D011278:Pregnanes