title: Effect of hydrophilicity of room temperature ionic liquids on the electrochemical and electrocatalytic behaviour of choline oxidase.
abstract: In the present report, six different nano-composites contaning the same amine functionalized multi-walled carbon nanotubes (NH(2)-MWCNTs) but different room temperature ionic liquids (RTILs) were prepared. Then, the efficiency of these nano-composites as supporting materials for studying the electrochemistry and electrocatalysis of choline oxidase (ChOx) as a model enzyme were compared. The corresponding cyclic voltammetric and amperometric data showed that the electrocatalytic activity and the electroanalytical performance of immobilized ChOx depends on the degree of hydrophilicity of RTILs used in the applied nano-composite. The higher stability (180 days), higher enzyme loading (6.56 mol cm(-2)), lower detection limit (3.85 μM) and wider linear range (0.005-0.8 mM) was obtained for the most hydrophilic RTIL (1-allyl-3-methylimidazolium bromide).
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Rahimi
authors.forename: Hedayatollah
authors.initials: H
authors.lastname: Ghourchian
authors.forename: Sharareh
authors.initials: S
authors.lastname: Sajjadi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D057927:Hydrophobic and Hydrophilic Interactions; D052578:Ionic Liquids / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013696:Temperature
chemical_list: D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D000429:Alcohol Oxidoreductases; C019888:choline oxidase