title: Isolation and molecular characterization of  Chikungunya virus  from the Andaman and Nicobar archipelago, India: evidence of an East, Central, and South African genotype.
abstract: Chikungunya virus (CHIKV) is an Alphavirus belonging to the family Togaviridae. In 2006, CHIKV infection struck the Andaman and Nicobar archipelago, with an attack rate of 60%. There were more than 10 cases with acute flaccid paralysis simulating the Guillian Barre Syndrome. The majority of the patients presented severe joint pain. The cause for such an explosive nature of the outbreak with increased morbidity was not known. The isolation of CHIKV was attempted and succeeded from nine subjects presenting clinical symptoms of Chikungunya fever. The cDNA of all the isolates was sequenced for partial E1 and nsP1 genes. Sequences were aligned based on the double locus sequence typing concept. The phylogenetic analysis shows that sequences of Andaman isolates grouped with the East, Central, and South African genotype of virus isolates from India, Sri Lanka, and Réunion. The genetic distance between Andaman isolates and the Réunion isolates was very small. The phylogenetic analysis confirmed the origin of the isolates responsible for the first ever confirmed CHIKV outbreak in these islands to be the East, Central, and South African genotype. In this manuscript, we discuss the involvement of the East, Central, and South African strain with the Chikungunya fever outbreak in this archipelago and double locus sequence typing as a first time approach.
authors.affiliation: Regional Medical Research Centre (ICMR), Indian Council of Medical Research, Post Bag-13, Port Blair-744 101, Andaman and Nicobar Islands.
authors.forename: N
authors.initials: N
authors.lastname: Muruganandam
authors.forename: I K
authors.initials: IK
authors.lastname: Chaaithanya
authors.forename: G S
authors.initials: GS
authors.lastname: Senthil
authors.forename: A N
authors.initials: AN
authors.lastname: Shriram
authors.forename: D
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: G S
authors.initials: GS
authors.lastname: Jeevabharathi
authors.forename: A B
authors.initials: AB
authors.lastname: Sudeep
authors.forename: N
authors.initials: N
authors.lastname: Pradeepkumar
authors.forename: P
authors.initials: P
authors.lastname: Vijayachari
mesh_terms: D018354:Alphavirus Infections / Q000821:virology; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D005843:Geography; D006801:Humans; D007194:India; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins