title: [Characteristics of Vibrio cholerae nonO1/nonO139 serogroup strains that caused diseases in population of Rostov region].
abstract: OBJECTIVE
  Genotype characteristic and determination of serological properties of Vibrio cholerae nonO1/nonO139 strains that caused diseases in population of Rostov region from 2000 to 2009.
  
  
  METHODS
  15 clinical strains of V. cholerae nonO1/nonO139 were studied. Serotyping was performed by using a kit of monospecific typing sera against serogroup 02-084 cholera vibrios obtained from Rostov Research Institute for Plague Control, PCR and VNTR-genotyping--by using specific primers described in scientific publications and constructed by us.
  
  
  RESULTS
  Serologic features of strains are very diverse and strains contain various combination of pathogenicity factor genes that seem to be interchangeable. Similar pattern was observed for VNTR-genotyping. Distribution of the examined strains by VNTR-genotyping did not correlate with either PCR-genotyping data or serotyping, or place and time of isolation.
  
  
  CONCLUSIONS
  The data obtained indicates a lack of general source of human infection even in the same location and time period. On the other hand, serological and genotypic features of V. cholerae nonO1/nonO139 may undergo changes in the process of staying in the macro organism or environment due to high plasticity of their genome.
authors.forename: V D
authors.initials: VD
authors.lastname: Kruglikov
authors.forename: E V
authors.initials: EV
authors.lastname: Monakhova
authors.forename: I V
authors.initials: IV
authors.lastname: Arkhangel'skaia
authors.forename: A S
authors.initials: AS
authors.lastname: Vodop'ianov
authors.forename: S A
authors.initials: SA
authors.lastname: Vodop'ianov
authors.forename: E P
authors.initials: EP
authors.lastname: Avdeeva
authors.forename: N V
authors.initials: NV
authors.lastname: Bozhko
authors.forename: L M
authors.initials: LM
authors.lastname: Smolikova
mesh_terms: D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics; D004196:Disease Outbreaks; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D012703:Serotyping; D041242:Vibrio cholerae O139 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D044163:Vibrio cholerae non-O1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors; D002772:Cholera Toxin