title: Application of a molecularly imprinted polymer for the extraction of kukoamine a from potato peels.
abstract: A molecularly imprinted polymer (MIP) for the purification of N(1),N(12)-bis(dihydrocaffeoyl)spermine (kukoamine A) was computationally designed and tested. The properties of the polymer were characterized. The protocol of the solid phase extraction (SPE) of kukoamine A from potato peels was optimized. A HPLC-MS method for the quantification of kukoamine A was developed and used for all optimization studies. The capacity of the MIP in relation to kukoamine A from the potato peels extract was estimated at 54 mg/g of the polymer. The kukoamine A purified from potato extract using MIP was exceptionally pure (≈ 90%). Although the corresponding blank polymer was less selective than the MIP for the extraction of kukoamine A from the potato extract, it was shown that the blank polymer could be effectively used for the purification of the crude synthetic kukoamine (polymer capacity = 80 mg of kukoamine A/g of the adsorbent, kukoamine A purity ≈ 86%). Therefore, selective adsorbents could be computationally designed for other plant products, allowing their purification in quantities that would be sufficient for more detailed studies and potential practical applications.
authors.affiliation: Cranfield Health, Cranfield University, Cranfield, Bedfordshire MK43 0AL, United Kingdom. piletska@cranfield.ac.uk
authors.forename: Elena V
authors.initials: EV
authors.lastname: Piletska
authors.forename: Rosemary
authors.initials: R
authors.lastname: Burns
authors.forename: Leon A
authors.initials: LA
authors.lastname: Terry
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Piletsky
mesh_terms: D054802:Molecular Imprinting; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013096:Spermine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D011108:Polymers; C096274:kukoamine A; D013096:Spermine