title: Quantitative proteomic analysis of membrane proteins involved in astroglial differentiation of neural stem cells by SILAC labeling coupled with LC-MS/MS.
abstract: Membrane proteins play a critical role in the process of neural stem cell self-renewal and differentiation. Here, we apply the SILAC (stable isotope labeling by amino acids in cell culture) approach to quantitatively compare the membrane proteome of the self-renewing and the astroglial differentiating cells. High-resolution analysis on a linear ion trap-Orbitrap instrument (LTQ-Orbitrap) at sub-ppm mass accuracy resulted in confident identification and quantitation of more than 700 distinct membrane proteins during the astroglial differentiation. Of the 735 quantified proteins, seven cell surface proteins display significantly higher expression levels in the undifferentiated state membrane compared to astroglial differentiating membrane. One cell surface protein transferrin receptor protein 1 may serve as a new candidate for NSCs surface markers. Functional clustering of differentially expressed proteins by Ingenuity Pathway Analysis revealed that most of overexpressed membrane proteins in the astroglial differentiation neural stem cells are involved in cellular growth, nervous system development, and energy metabolic pathway. Taken together, this study increases our understanding of the underlying mechanisms that modulate complex biological processes of neural stem cell proliferation and differentiation.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University , Changsha 410081, PR China. caor@hunnu.edu.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Zang
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Xianchun
authors.initials: X
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002461:Cell Line, Transformed; D002853:Chromatography, Liquid; D007553:Isotope Labeling; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D020543:Proteome