title: Frequency of hepatitis C viral RNA in anti-hepatitis C virus non- reactive blood donors with raised alanine aminotransferase.
abstract: The objective of this study was to find out the frequency of HCV RNA in anti-HCV non-reactive blood donors with raised alanine amino transferase (ALT). The study was conducted at Baqai Institute of Haematology, Baqai Medical University, Karachi, in collaboration with Combined Military Hospital, Malir Cantt, Karachi. The demographic data of blood donors was recorded, and anti-HCV, HBsAg and HIV were screened. Four hundred consecutive donors with raised ALT above the reference range were included in study. HCV RNA RT-PCR was performed on 5 sample minipools using Bio-Rad Real time PCR equipment. HCV RNA was detected in 1/400 (0.25%) blood donors. Finding of raised ALT in blood donors warrants further investigations. In case, if raised ALT is unexplained presence of HCV RNA may be suspected.
authors.affiliation: Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi. dr.nadir.ali@gmail.com
authors.forename: Nadir
authors.initials: N
authors.lastname: Ali
authors.forename: Syed Azhar
authors.initials: SA
authors.lastname: Ahmed
authors.lastname: Moinuddin
authors.forename: Susan Fisher
authors.initials: SF
authors.lastname: Hoch
authors.forename: Rashid A
authors.initials: RA
authors.lastname: Chotani
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006526:Hepatitis C / Q000097:blood; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012698:Serologic Tests; D055815:Young Adult
chemical_list: D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D000410:Alanine Transaminase