title: Induction of cytotoxicity of Pelagia noctiluca venom causes reactive oxygen species generation, lipid peroxydation induction and DNA damage in human colon cancer cells.
abstract: BACKGROUND
  The long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity of Pelagia noctiluca venom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects.
  
  
  RESULTS
  Our results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctiluca induced DNA fragmentation. SDS-PAGE analysis of Pelagia noctiluca venom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa.
  
  
  CONCLUSIONS
  Oxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctiluca venom.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Ayed
authors.forename: Manel
authors.initials: M
authors.lastname: Boussabbeh
authors.forename: Wiem
authors.initials: W
authors.lastname: Zakhama
authors.forename: Chayma
authors.initials: C
authors.lastname: Bouaziz
authors.forename: Salwa
authors.initials: S
authors.lastname: Abid
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003064:Cnidarian Venoms / Q000302:isolation & purification / Q000494:pharmacology*; D003110:Colonic Neoplasms; D003603:Cytotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D053938:DNA Fragmentation; D045325:HCT116 Cells; D006801:Humans; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D037401:Scyphozoa / Q000737:chemistry*
chemical_list: D003064:Cnidarian Venoms; D003603:Cytotoxins; D017382:Reactive Oxygen Species; D002374:Catalase