title: The effect of progesterone in the prevention of the chemically induced experimental colitis in rats.
abstract: OBJECTIVE
  To study the effects of progesterone on an experimental colitis model.
  
  
  METHODS
  Wistar albino rats were treated subcutaneously with 2mg/kg once a day during seven days Colitis was induced by intrarectal administration of 5mg trinitrobenzene sulfonic acid (TNBS). Disease activities, macroscopic and microscopic scores were evaluated. To determine the response provoked by progesterone we measured Colonic malondialdehyde (MDA), TNF alfa, IL-6 and Nitric oxide (NO) levels in addition to the MPO (Myeloperoxidase) and caspase-3 activities.
  
  
  RESULTS
  Progesterone ameliorated significantly the macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic MDA levels and caspase-3 activities in group 2 in comparison to the control group. The results of the study revealed a decline in MDA, NO, IL6 and TNF-α levels in the colon tissue and in blood due to progesterone therapy in group 3 when compared to the group 2, a significant improvement. Progesterone treatment was associated with decreased MDA, MPO, TNF alfa and caspase-3 activity.
  
  
  CONCLUSIONS
  Progesterone therapy decreased oxidative damage in the colonic mucosa.
authors.affiliation: Okmeydani Research Hospital General, Surgery Department, Istanbul, Turkey. drkaratepe@yahoo.com
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Karatepe
authors.forename: Merih
authors.initials: M
authors.lastname: Altiok
authors.forename: Muharrem
authors.initials: M
authors.lastname: Battal
authors.forename: Gulcin
authors.initials: G
authors.lastname: Kamali
authors.forename: Ahu
authors.initials: A
authors.lastname: Kemik
authors.forename: Timucin
authors.initials: T
authors.lastname: Aydin
authors.forename: Servet
authors.initials: S
authors.lastname: Karahan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control*; D003106:Colon / Q000737:chemistry / Q000187:drug effects*; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D007413:Intestinal Mucosa / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis; D011374:Progesterone / Q000627:therapeutic use*; D011372:Progestins / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D011372:Progestins; D009569:Nitric Oxide; D011374:Progesterone; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid