title: Moderate or deep local hypothermia does not prevent the onset of ischemia-induced dendritic damage.
abstract: We studied the acute (up to 2 hours after reperfusion) effects of localized cortical hypothermia on ischemia-induced dendritic structural damage. Moderate (31°C) and deep (22°C) hypothermia delays, but does not block the onset of dendritic blebbing or spine loss during global ischemia in mouse in vivo. Hypothermic treatment promoted more consistent recovery of dendritic structure and spines during reperfusion. These results suggest that those using therapeutic hypothermia will need to consider that it does not spare neurons from structural changes that are the result of ischemia, but hypothermia may interact with mechanisms that control the onset of damage and recovery during reperfusion.
authors.affiliation: Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Sherri
authors.initials: S
authors.lastname: Tran
authors.forename: Shangbin
authors.initials: S
authors.lastname: Chen
authors.forename: Ran R
authors.initials: RR
authors.lastname: Liu
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Xie
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001831:Body Temperature; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D003712:Dendrites / Q000473:pathology*; D049229:Dendritic Spines / Q000473:pathology; D004195:Disease Models, Animal; D007036:Hypothermia, Induced / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D020521:Stroke / Q000150:complications* / Q000473:pathology; D013997:Time Factors