title: Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.
abstract: Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å(3) Da(-1); assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å(3) Da(-1).
authors.affiliation: Biomolecular Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Momma
authors.forename: Hee Kwon
authors.initials: HK
authors.lastname: Kang
authors.forename: Kazumi
authors.initials: K
authors.lastname: Funane
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003910:Dextranase / Q000737:chemistry*; D013295:Streptococcus mutans / Q000201:enzymology*
chemical_list: D003910:Dextranase