title: Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.
abstract: In this unit, an efficient method for the synthesis of 2'-tellerium-modified phosphoramidite and its incorporation into oligonucleotide are presented. We choose 5'-O-DMTr-2,2'-anhydro-uridine and -thymidine nucleosides (S.1, S.2) as starting materials due to their easy preparation. The 5'-O-DMTr-2,2'-anhydro-uridine and -thymidine can be converted to the corresponding 2'-tellerium-derivatized nucleosides by treating with the telluride nucleophiles. Subsequently, the 2'-Te-nucleosides can be transformed into 3'-phosphoramidites, which are the building blocks for DNA/RNA synthesis. The DNA synthesis, purification, and applications of oligonucleotides containing 2'-Te-U or 2'-Te-T are described in the protocol.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia, USA.
authors.forename: Sibo
authors.initials: S
authors.lastname: Jiang
authors.forename: Jia
authors.initials: J
authors.lastname: Sheng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D004247:DNA / Q000737:chemistry / Q000235:genetics; D008956:Models, Chemical; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA; D013691:Tellurium