title: Preparation and biological effect of nucleotide-capped CdSe/ZnS quantum dots on Tetrahymena thermophila.
abstract: In this paper, we described the preparation and characterization of different types of modified CdSe/ZnS quantum dots (QDs) and explored the biological effects of QDs with different surface modifications on the whole growth of unicellular protozoan Tetrahymena thermophila BF(5) using a thermal activity monitor air isothermal microcalorimeter. Our results demonstrated that adenosine 5'-monophosphate (AMP) showed stronger interaction with QDs than other types of nucleotide. AMP-QDs could stimulate the growth of T. thermophila while mercaptoacetic acid-capped CdSe/ZnS quantum dots inhibited it. In addition, the population density determination and fluorescence imaging of T. thermophila BF(5) also confirmed the results obtained from microcalorimetry. It is believed that this approach will provide a more convenient methodology for the kinetics and thermodynamics of microorganism when coexisting with QDs in real time, and all of which are very significant to understanding the effect of QDs to organism.
authors.affiliation: College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Ting
authors.initials: T
authors.lastname: Qiu
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry / Q000494:pharmacology; D019187:Cadmium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009711:Nucleotides / Q000737:chemistry* / Q000494:pharmacology; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry* / Q000494:pharmacology; D016808:Tetrahymena thermophila / Q000187:drug effects / Q000254:growth & development*; D013816:Thermodynamics; D013864:Thioglycolates / Q000737:chemistry / Q000494:pharmacology; D017967:Zinc Compounds / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D019187:Cadmium Compounds; D009711:Nucleotides; D018036:Selenium Compounds; D013440:Sulfides; D013864:Thioglycolates; D017967:Zinc Compounds; D000249:Adenosine Monophosphate; C017487:2-mercaptoacetate; C058667:cadmium selenide; C031238:zinc sulfide