title: The dynamic expression of allograft inflammatory factor-1 in hepatic tissues and splenic cells of BALB/c mice with Schistosoma japonicum infection.
abstract: Allograft inflammatory factor-1 (AIF-1) was originally cloned from a rat heart allograft under chronic rejection. Data from many studies suggested an important role of AIF-1 in several inflammatory processes. The aim of this study was to examine the dynamic expression of AIF-1 and its association with the pathogenesis of hepatic schistosomiasis in BALB/c mice infected with S. japonicum. The expression of AIF-1 and tumour necrosis factor-alpha (TNF-α) was determined by enzyme-linked immunosorbent assay, western blot and immunohistochemistry. AIF-1 and TNF-α were overexpressed in hepatic tissues at the early stage of infection, and then diminished with the length of infection. On culturing splenocytes stimulated by soluble egg antigen for 72 h, the expression of AIF-1 in infected mice was suppressed, but TNF-α increased gradually. Our results showed that AIF-1 was overexpressed in the liver of BALB/c mice infected with S. japonicum, and the interaction between AIF-1 and TNF-α or other cytokines played an important role in the pathogenesis and progression of hepatic schistosomiasis.
authors.affiliation: Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Q R
authors.initials: QR
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Guan
authors.forename: D J
authors.initials: DJ
authors.lastname: Yan
authors.forename: D S
authors.initials: DS
authors.lastname: Lei
authors.forename: L
authors.initials: L
authors.lastname: Fu
authors.forename: H S
authors.initials: HS
authors.lastname: Xia
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhu
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
authors.forename: A O
authors.initials: AO
authors.lastname: Niu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000378:metabolism / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000096:biosynthesis*; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D013154:Spleen / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: C491049:Aif1 protein, mouse; D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D014409:Tumor Necrosis Factor-alpha