title: Development of the first sphingomyelin biomimetic stationary phase for immobilized artificial membrane (IAM) chromatography.
abstract: A prototype sphingomyelin stationary phase for Immobilized Artificial Membrane (IAM) chromatography was synthesized by an ultra-short, solid-phase inspired methodology, in which an oxidative release monitoring strategy played a vital role. Evaluated in a proof-of-concept model for blood-brain barrier passage, partial least squares regression demonstrated its potential as an in vitro prediction tool.
authors.affiliation: Pfizer Analytical Research Centre, Department of Organic Chemistry, Ghent University, Krijgslaan 281-S4bis, B-9000 Ghent, Belgium.
authors.forename: Dieter
authors.initials: D
authors.lastname: Verzele
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lynen
authors.forename: Mike
authors.initials: M
authors.lastname: De Vrieze
authors.forename: Adrian G
authors.initials: AG
authors.lastname: Wright
authors.forename: Melissa
authors.initials: M
authors.lastname: Hanna-Brown
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001812:Blood-Brain Barrier / Q000378:metabolism; D002845:Chromatography / Q000379:methods*; D016018:Least-Squares Analysis; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D013109:Sphingomyelins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D013109:Sphingomyelins