title: Peptoniphilus duerdenii sp. nov. and Peptoniphilus koenoeneniae sp. nov., isolated from human clinical specimens.
abstract: Two previously uncharacterized strains of Gram-reaction-positive, anaerobic, coccus-shaped bacteria, designated strains WAL 18896(T) and WAL 18898(T), were recovered from human wound specimens and characterized using phenotypic, chemotaxonomic and molecular taxonomic methods. Comparative 16S rRNA gene sequence analysis and chemotaxonomic and biochemical characteristics demonstrated that these organisms are genotypically and phenotypically distinct and represent previously unidentified sublines within the order Clostridiales in the phylum Firmicutes. Pairwise sequence analysis demonstrated that the novel organisms had 91.9% sequence similarity to each other and were most closely related to members of the genus Peptoniphilus. The major long-chain fatty acids of both strains were C(16:0,) C(18:0), C(18:1)ω9c and C(18:2)ω6,9c. Based on the phenotypic and phylogenetic findings, strains WAL 18896(T) ( = CCUG 56065(T)  = ATCC BAA-1640(T)) and WAL 18898(T) ( = CCUG 56067(T)  = ATCC BAA-1638(T)  = DSM 22616(T)) represent two novel species, for which the names Peptoniphilus duerdenii sp. nov. and Peptoniphilus koenoeneniae sp. nov. are proposed, respectively.
authors.affiliation: Marmara University, Faculty of Medicine, Department of Microbiology, Istanbul, Turkey.|Research Service, VA Medical Center West Los Angeles, CA, USA.
authors.forename: Nurver
authors.initials: N
authors.lastname: Ulger-Toprak
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
authors.affiliation: Research Service, VA Medical Center West Los Angeles, CA, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Summanen
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: O'Neal
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, CA, USA.|Department of Medicine, UCLA School of Medicine, Los Angeles, CA, USA.|Infectious Diseases Section, VA Medical Center, West Los Angeles, CA, USA.
authors.forename: Sydney M
authors.initials: SM
authors.lastname: Finegold
mesh_terms: D000038:Abscess / Q000382:microbiology*; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S