title: High isoprenoid flux Escherichia coli as a host for carotenoids production.
abstract: A noncarotenogenic microbe E. coli was engineered for high production of carotenoids. To increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (dxs, idi and ispDispF) in the isoprenoid pathway were replaced with a strong bacteriophage T5 promoter (P(T5)) by using the λ-Red recombinase system in combination with the Flp/FRT site-specific recombination system for marker excision and P1 transduction for gene trait stacking. The resulting high isoprenoid flux E. coli can be used as a starting strain to produce various carotenoids by introducing heterologous carotenoid genes. In this study, the high isoprenoid flux E. coli was transformed with a plasmid carrying the β-carotene biosynthetic genes from Pantoea stewartii for β-carotene production.
authors.affiliation: DuPont Central Research and Development, Wilmington, DE, USA. wonchul.suh@usa.dupont.com
authors.forename: Wonchul
authors.initials: W
authors.lastname: Suh
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D005818:Genetic Engineering / Q000379:methods; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013729:Terpenes / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D013729:Terpenes; D002338:Carotenoids