title: Altered dendritic cell-natural killer interaction in Kenyan sex workers resistant to HIV-1 infection.
abstract: BACKGROUND
  Natural killer (NK) cells are members of the innate immune system that play an important role in the defense against viral infection. They are also involved in the regulation of adaptive immune responses through cytokine secretion and the interaction with antigen-presenting cells. However, their role in HIV infection is only partially understood.
  
  
  OBJECTIVE
  Here we studied the phenotype and function of NK cells of highly HIV-exposed but seronegative (HESN) uninfected commercial sex workers from Kenya who can be epidemiologically defined as relatively resistant to HIV infection.
  
  
  METHODS
  The purpose of this study was to gain insight into the role of NK cells in mediating resistance to HIV-1. This information can be used to better understand protection from infection which can be used for informing future design of effective prophylactics and therapeutics for HIV.
  
  
  METHODS
  Whole blood samples were collected from study participants and isolated NK cells and dendritic cells were used in assays for phenotyping and cell function.
  
  
  RESULTS
  Activated NK cells from resistant women killed autologous immature dendritic cells more efficiently and also secreted more interferon (IFN)-γ than those of uninfected, susceptible women. Interestingly, NK cells from HIV-resistant women were significantly more effective in inducing secretion of IL-12 in immature dendritic cells.
  
  
  CONCLUSIONS
  These data suggest that an altered NK cell-dendritic cell interaction plays an important role in the protection from infection with HIV-1.
authors.affiliation: The Lautenberg Center for General and Tumor Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel.
authors.forename: Hormas
authors.initials: H
authors.lastname: Ghadially
authors.forename: Yoav
authors.initials: Y
authors.lastname: Keynan
authors.forename: Joshua
authors.initials: J
authors.lastname: Kimani
authors.forename: Makobu
authors.initials: M
authors.lastname: Kimani
authors.forename: T Blake
authors.initials: TB
authors.lastname: Ball
authors.forename: Francis A
authors.initials: FA
authors.lastname: Plummer
authors.forename: Ofer
authors.initials: O
authors.lastname: Mandelboim
authors.forename: Adrienne F A
authors.initials: AF
authors.lastname: Meyers
mesh_terms: D000328:Adult; D017252:CD3 Complex / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015483:HIV Antibodies / Q000276:immunology; D018023:HIV Seronegativity / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D016130:Immunophenotyping; D007630:Kenya / Q000453:epidemiology; D007694:Killer Cells, Natural / Q000276:immunology*; D016273:Occupational Exposure / Q000706:statistics & numerical data; D060386:Sex Workers / Q000706:statistics & numerical data
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D015483:HIV Antibodies