title: Influence of atrial fibrillation on microRNA expression profiles in left and right atria from patients with valvular heart disease.
abstract: Chronic atrial fibrillation (AF) is a complication associated with the dilated atria of patients with valvular heart disease and contributes to worsened pathology. We examined microRNA (miRNA) expression profiles in right and left atrial appendage tissue from valvular heart disease (VHD) patients. Right atrial (RA) appendage from patients undergoing coronary artery bypass grafting and left atrial (LA) appendage from healthy hearts, not used for transplant, were used as controls. There was no detectable effect of chronic AF on miRNA expression in LA tissue, but miRNA expression in RA was strongly influenced by AF, with 47 miRNAs (15 higher, 32 lower) showing differential expression between the AF and control sinus rhythm groups. VHD induced different changes in miRNA expression in LA compared with RA. Fifty-three (12 higher, 41 lower) miRNAs were altered by VHD in LA, compared with 5 (4 higher, 1 lower) in RA tissue. miRNA profiles also differed between VHD-LA and VHD-RA (13 higher, 26 lower). We conclude that VHD and AF influence miRNA expression patterns in LA and RA, but these are affected differently by disease progression and by the development of AF. These findings provide new insights into the progression of VHD.
authors.affiliation: Molecular Cardiology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne 8008, Victoria, Australia.
authors.forename: Nicola
authors.initials: N
authors.lastname: Cooley
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Cowley
authors.forename: Ruby C Y
authors.initials: RC
authors.lastname: Lin
authors.forename: Silvana
authors.initials: S
authors.lastname: Marasco
authors.forename: Chiew
authors.initials: C
authors.lastname: Wong
authors.forename: David M
authors.initials: DM
authors.lastname: Kaye
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Dart
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Woodcock
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D020517:Atrial Appendage / Q000378:metabolism*; D001281:Atrial Fibrillation / Q000209:etiology* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006349:Heart Valve Diseases / Q000150:complications* / Q000503:physiopathology; D006801:Humans; D016014:Linear Models; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014739:Victoria
chemical_list: D035683:MicroRNAs