title: Isolation of hepatic progenitor cells from the galactosamine-treated rat liver.
abstract: Oval cells and small hepatocytes (SHs) are well known as hepatic stem/progenitor cells. However, the relationship between the oval cells and SHs in liver regeneration is not well understood. To resolve this issue, we established a technique to selectively separate oval cells and SHs. In the injured rat liver, oval cells and SHs transiently appear in the initial period of liver regeneration. Thy1(+) and CD44(+) cells are candidates for markers of oval cells and SHs, respectively. In this chapter, the methods for sorting and culture of the cells are described in detail.
authors.affiliation: Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan. nichi@sapmed.ac.jp
authors.forename: Norihisa
authors.initials: N
authors.lastname: Ichinohe
authors.forename: Junko
authors.initials: J
authors.lastname: Kon
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mitaka
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005688:Galactosamine / Q000008:administration & dosage / Q000633:toxicity; D022781:Hepatocytes / Q000166:cytology*; D018960:Hyaluronan Receptors / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000293:injuries*; D008115:Liver Regeneration / Q000502:physiology*; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018960:Hyaluronan Receptors; D018800:Thy-1 Antigens; D005688:Galactosamine