title: Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels.
abstract: In this work, we reported a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor for carcinoembryonic antigen (CEA) on a gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The Ru-silica (Ru(bpy)(3)(2+)-doped silica) capped nanoporous gold (NPG) (Ru-silica@NPG) composite was used as an excellent label with amplification techniques. The NPG was prepared with a simple dealloying strategy, by which silver was dissolved from silver/gold alloys in nitric acid. The primary antibody was immobilized on the AuNPs modified electrode through l-cysteine and glutaraldehyde, and then the antigen and the functionalized Ru-silica@NPG composite labeled secondary antibody were conjugated successively to form a sandwich-type immunocomplex through the specific interaction. The concentrations of CEA were obtained in the range from 1 pg mL(-1) to 10 ng mL(-1) with a detection limit of 0.8 pg mL(-1). The as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan, 250022, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Weiping
authors.initials: W
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000032:analysis; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D053768:Metal Nanoparticles; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D006046:Gold; D012822:Silicon Dioxide; D012428:Ruthenium