title: Identification and characterization of a mycobacterial (2R,3R)-2,3-butanediol dehydrogenase.
abstract: Bacterial strain B-009, capable of using racemic 1,2-propanediol (PD), was identified as a rapid-growing member of the genus Mycobacterium. The strain is phylogenetically related to M. gilvum, but has slightly different physiological characteristics. An NAD(+)-dependent enantioselective alcohol dehydrogenase, which acts on R-PD, was purified from the strain. The enzyme was a homodimer of a peptide coded by a 1047-bp gene (mbd1). A highly conserved sequence for medium-chain dehydrogenase/reductases with a preference for secondary alcohols was found in the gene. Hydroxyacetone was produced from R-PD by an enzymatic reaction, indicating that position 2 of the substrate was oxidized. The enzyme activity was highest for (2R,3R)-2,3-butanediol (R,R-BD), enabling the enzyme to be identified as (2R,3R)-2,3-butanediol dehydrogenase (R,R-BD-DH). A homology search revealed M. gilvum, M. vanbaalenii, and M. semegmatis to have ORFs similar to mbd1, suggesting the widespread distribution of genes encoding R,R-BD-DH among mycobacterial strains.
authors.affiliation: Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University, Japan. mtake@ynu.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Muranushi
authors.forename: Sawako
authors.initials: S
authors.lastname: Inagaki
authors.forename: Takuya
authors.initials: T
authors.lastname: Nakao
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Motomatsu
authors.forename: Ichiro
authors.initials: I
authors.lastname: Suzuki
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Koizumi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D019946:Propylene Glycol / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D019946:Propylene Glycol; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase