title: Crystallization of a 79 kDa fragment of the hook protein FlgE from Campylobacter jejuni.
abstract: A 79 kDa fragment of the bacterial flagellar hook protein FlgE from Campylobacter jejuni was cloned, overexpressed, purified and crystallized. Two different crystal forms were obtained. Synchrotron X-ray diffraction data showed that the first crystal form, which diffracted to 4.9 Å resolution, belonged to the tetragonal crystal system, with space group I4(1)22 and unit-cell parameters a = b = 186.2, c = 386.6 Å, α = β = γ = 90°. The second crystal form diffracted to 2.5 Å resolution and belonged to the monoclinic crystal system, with space group P2(1) and unit-cell parameters a = 75.7, b = 173.8, c = 150.8 Å, α = γ = 90, β = 106.5°. SeMet protein was also overexpressed, purified and crystallized, and a 2.6 Å resolution MAD data set was collected.
authors.affiliation: Trans-membrane Trafficking Unit, Okinawa Institute of Science and Technology, Kunigami-gun, Okinawa, Japan.
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Kido
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Yoon
authors.forename: Fadel A
authors.initials: FA
authors.lastname: Samatey
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D016123:Campylobacter jejuni / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; C097681:FlgE protein, Bacteria