title: Experimental approaches to the study of human primordial germ cells.
abstract: The survival, proliferation, and differentiation of primordial germ cells in the mammalian embryo is regulated by a complex cocktail of growth factors and interactions with surrounding somatic cells, which together form a microenvironment known as the germ cell niche. Extensive insight into the signalling pathways that regulate PGC behaviour has been provided by the study of these cells in rodent models, however little is known about the factors that regulate these processes in human PGCs. In this review, we outline experimental approaches to the culture and manipulation of the first trimester human fetal ovary, and discuss immunohistochemical and stereological approaches to detect changes in human PGC numbers and proliferation in response to treatment with exogenous growth factors.
authors.affiliation: MRC Centre for Reproductive Health, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK. achilds@staffmail.ed.ac.uk
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Childs
authors.forename: Richard A
authors.initials: RA
authors.lastname: Anderson
mesh_terms: D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D004210:Dissection / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D005854:Germ Cells / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009924:Organ Culture Techniques / Q000379:methods; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D011247:Pregnancy; D061251:Primary Cell Culture / Q000379:methods; D012732:Sex Determination Analysis / Q000379:methods