title: Investigating the origins of somatic cell populations in the perinatal mouse ovaries using genetic lineage tracing and immunohistochemistry.
abstract: Genetic lineage tracing (or fate mapping) techniques are designed to permanently label progenitor cells of target tissues, thereby allowing delineation of the progenies of labeled cells during organogenesis. This technology has been widely used in the study of cell migration and lineage specification in various organs and organisms. Here, we describe how to apply the genetic lineage tracing model in combination with immunohistochemistry to identify the potential origins of somatic cell precursors in perinatal mouse ovaries.
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.forename: Melissa
authors.initials: M
authors.lastname: Paczkowski
authors.forename: Manal
authors.initials: M
authors.lastname: Othman
authors.forename: Humphrey Hung-Chang
authors.initials: HH
authors.lastname: Yao
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods; D019070:Cell Lineage / Q000235:genetics*; D060965:Clonal Evolution; D005260:Female; D060005:Genotyping Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009924:Organ Culture Techniques / Q000379:methods; D038081:Organogenesis; D010053:Ovary / Q000196:embryology*; D011247:Pregnancy; D013629:Tamoxifen / Q000008:administration & dosage
chemical_list: D013629:Tamoxifen