title: Statistical optimization of polysaccharide production by submerged cultivation of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W.Curt.: Fr.) P. Karst. MTCC 1039 (Aphyllophoromycetideae).
abstract: Response surface methodology was employed to optimize the concentration of four important cultivation media components such as cottonseed oil cake, glucose, NH4Cl, and MgSO4 for maximum medicinal polysaccharide yield by Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum MTCC 1039 in submerged culture. The second-order polynomial model describing the relationship between media components and polysaccharide yield was fitted in coded units of the variables. The higher value of the coefficient of determination (R2 = 0.953) justified an excellent correlation between media components and polysaccharide yield, and the model fitted well with high statistical reliability and significance. The predicted optimum concentration of the media components was 3.0% cottonseed oil cake, 3.0% glucose, 0.15% NH4Cl, and 0.045% MgSO4, with the maximum predicted polysaccharide yield of 819.76 mg/L. The experimental polysaccharide yield at the predicted optimum media components was 854.29 mg/L, which was 4.22% higher than the predicted yield.
authors.affiliation: Department of Biotechnology, St. Joseph's College of Engineering, Chennai, India. basg2004@gmail.com
authors.forename: Gurunathan
authors.initials: G
authors.lastname: Baskar
authors.forename: Shree Rajesh K Lakshmi Jai
authors.initials: SR
authors.lastname: Sathya
authors.forename: Riswana Begum
authors.initials: RB
authors.lastname: Jinnah
authors.forename: Renganathan
authors.initials: R
authors.lastname: Sahadevan
mesh_terms: D000643:Ammonium Chloride / Q000378:metabolism; D003369:Cottonseed Oil / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D008278:Magnesium Sulfate / Q000378:metabolism; D015233:Models, Statistical; D011134:Polysaccharides / Q000096:biosynthesis*; D020940:Reishi / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D003369:Cottonseed Oil; D003470:Culture Media; D011134:Polysaccharides; D000643:Ammonium Chloride; D008278:Magnesium Sulfate; D005947:Glucose