title: Significance of complement components C1q and C4 bound to circulating immune complexes in juvenile idiopathic arthritis: support for classical complement pathway activation.
abstract: OBJECTIVE
  Immune complexes (ICs) from sera of juvenile idiopathic arthritis (JIA) patients show increased complement opsonisation; however, a definitive role for involvement of the classical or alternative pathway is not entirely clear. To delineate the role of these pathways, we measured activated complement products bound to circulating IC (CICs) in the sera of JIA patients.
  
  
  METHODS
  Sera from 100 JIA patients and 22 healthy children were collected. C1q, C4, C3, C3d, and membrane attack complex (MAC) bound to CICs were measured by enzyme-linked immunosorbent assay. Data was compared to IgM rheumatoid factor (RF), IgG anti-cyclic citrullinated peptide (CCP) antibodies, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) levels.
  
  
  RESULTS
  Mean levels of C1q, C4, and MAC bound to CICs were significantly elevated in JIA patients compared to healthy children. C1q correlated significantly with C4 and MAC bound to CICs and C4 and MAC also demonstrated significant correlation. No significant differences were noted in complement components bound to CICs when evaluating IgM RF, anti-CCP antibody, and CRP positivity. A significant correlation was noted between MAC bound to CICs and ESR. C1q and MAC bound to CICs mean levels were significantly higher in patients with an elevated ESR compared to those with a normal ESR level.
  
  
  CONCLUSIONS
  JIA patients have elevated levels of complement components bound to CICs, particularly from the classical pathway. Moreover, classical pathway components were associated with ESR, a marker of disease activity. MAC bound to CICs also correlated significantly with ESR, further supporting the notion of complement-mediated tissue injury that is triggered by IC-mediated classical pathway activation.
authors.affiliation: Saint Louis University School of Medicine, Saint Louis, MO, USA.
authors.forename: Brooke E
authors.initials: BE
authors.lastname: Gilliam
authors.forename: Melinda R
authors.initials: MR
authors.lastname: Reed
authors.forename: Anil K
authors.initials: AK
authors.lastname: Chauhan
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Dehlendorf
authors.forename: Terry L
authors.initials: TL
authors.lastname: Moore
mesh_terms: D000936:Antigen-Antibody Complex / Q000097:blood / Q000276:immunology*; D001168:Arthritis / Q000097:blood / Q000276:immunology / Q000473:pathology; D001171:Arthritis, Juvenile / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002648:Child; D003167:Complement Activation; D015922:Complement C1q / Q000032:analysis / Q000276:immunology*; D003181:Complement C4 / Q000032:analysis / Q000276:immunology*; D003171:Complement Pathway, Classical / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D011485:Protein Binding
chemical_list: D000936:Antigen-Antibody Complex; D003181:Complement C4; D015922:Complement C1q