title: Cytogenetic in vivo assays in somatic cells.
abstract: Chromosome aberration assays are employed to detect the induction of chromosome breakage (clastogenesis) in somatic and germ cells by direct observation of the chromosomal damage during metaphase analysis, or by indirect observation of chromosomal fragments. Thus, various types of cytogenetic change can be detected such as structural chromosome aberrations (CA), sister chromatid exchanges (SCE), ploidy changes, and micronuclei. Following the induction of the chromosomal damage, most of the aberrations and abnormalities detected by these assays can be detrimental or even lethal to the cell. Their presence, however, indicates a potential to also induce more subtle and therefore transmissible chromosomal damage which survives cell division to produce heritable cytogenetic changes. Usually, induced cytogenetic damage is accompanied by other genotoxic damage such as gene mutations.
authors.affiliation: Safety Assessment, AstraZeneca, Cheshire, UK. Ann.Doherty@astrazeneca.com
authors.forename: Ann T
authors.initials: AT
authors.lastname: Doherty
authors.forename: Adi
authors.initials: A
authors.lastname: Baumgartner
authors.forename: Diana
authors.initials: D
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002503:Centromere / Q000235:genetics; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics; D020732:Cytogenetic Analysis / Q000379:methods*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests / Q000379:methods*; D051381:Rats