title: Effect of progesterone on the in vitro response of peripheral blood mononuclear cells stimulated by Escherichia coli in mares.
abstract: Escherichia coli(E. coli) isolated from the uterus of a Thoroughbred mare with bacterial endometritis was used to evaluate the effect of progesterone (P(4)) on the immune response of mares. Peripheral blood mononuclear cells (PBMCs) were collected from 10 nonpregnant clinically healthy adult mares (range, 4-12 years) during diestrus, four Thoroughbreds and six Hokkaido native horses. Cell proliferation and expression of cytokine mRNA, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-10, of PBMCs stimulated with E. coli and P(4) were examined in vitro. P(4) was shown to have significantly inhibited E. coli induced proliferation and expression of IFN-γ in PBMCs. These results indicate that P(4) inhibits the immune response to E. coli in mares.
authors.affiliation: School of Veterinary Medicine, Kitasato University, 35-1 Higashi 23 Bancho, Towada, Aomori 034-8628, Japan. dv10007j@st.kitasato-u.ac.jp
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Maeda
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Michiko
authors.initials: M
authors.lastname: Tomioka
authors.forename: Taishi
authors.initials: T
authors.lastname: Tanabe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nambo
authors.forename: Haruki
authors.initials: H
authors.lastname: Uematsu
authors.forename: Masa-aki
authors.initials: MA
authors.lastname: Oikawa
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D006736:Horses / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D011374:Progesterone