title: 6-Benzylaminopurine delays senescence and enhances health-promoting compounds of harvested broccoli.
abstract: The effect of 6-benzylaminopurine (6-BA) on the color, antioxidant activity, and contents of total phenols, glucosinolate, and sulforaphane in broccoli florets was investigated. The results showed that 6-BA treatment markedly inhibited the increase of the L* value and malondialdehyde (MDA) content and retarded the decrease of the H value. 6-BA treatment reduced the rate of chlorophyll degradation by regulating the activities of chlorophyllase and Mg-dechelatase. When compared to control florets, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were enhanced in florets treated with 6-BA, whereas the activity of peroxidase (POD) was significantly reduced. The contents of total phenols, glucosinolate, and sulforaphane in broccoli florets were also profoundly increased after treatment with 6-BA. These results indicated that 6-BA could maintain the quality, delay senescence, and improve the nutritional value of broccoli.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Yang
authors.forename: Xuehong
authors.initials: X
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001593:Benzyl Compounds; D001937:Brassica / Q000737:chemistry* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005519:Food Preservation / Q000379:methods*; D007701:Kinetin / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D009753:Nutritive Value; D010544:Peroxidases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011687:Purines
chemical_list: D000975:Antioxidants; D001593:Benzyl Compounds; D004798:Enzymes; D010940:Plant Proteins; D011687:Purines; C421174:magnesium dechelatase; D008315:Malondialdehyde; D010544:Peroxidases; D002374:Catalase; C480551:benzylaminopurine; D007701:Kinetin