title: The LIM domain protein, CRIP2, promotes apoptosis in esophageal squamous cell carcinoma.
abstract: The group 2 LIM domain protein, Cysteine-rich intestinal protein 2 (CRIP2) was found to play an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. Subcellular fractionation studies show that CRIP2 is expressed in the nucleus. Real-time quantitative PCR shows CRIP2 expression is down-regulated in ESCC tissues and cell lines. Functional studies reveal that CRIP2 reduces colony formation, growth, and invasion abilities. Furthermore, over-expression of CRIP2 induces apoptosis through induction of active caspases 3 and 9 proteins. In conclusion, this study shows CRIP2 plays an important role in the development of ESCC.
authors.affiliation: Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, HKSAR, Hong Kong, People's Republic of China.
authors.forename: Paulisally Hau Yi
authors.initials: PH
authors.lastname: Lo
authors.forename: Josephine Mun Yee
authors.initials: JM
authors.lastname: Ko
authors.forename: Zhuo You
authors.initials: ZY
authors.lastname: Yu
authors.forename: Simon
authors.initials: S
authors.lastname: Law
authors.forename: Li Dong
authors.initials: LD
authors.lastname: Wang
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Li
authors.forename: Gopesh
authors.initials: G
authors.lastname: Srivastava
authors.forename: Sai Wah
authors.initials: SW
authors.lastname: Tsao
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Stanbridge
authors.forename: Maria Li
authors.initials: ML
authors.lastname: Lung
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D015854:Up-Regulation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C486176:CRIP2 protein, human; D060588:LIM Domain Proteins; D053148:Caspase 3; D053453:Caspase 9