title: Investigations into matrix components affecting the performance of the official bioassay reference method for quantitation of paralytic shellfish poisoning toxins in oysters.
abstract: Significant differences previously observed in the determination of paralytic shellfish poisoning toxins (PSTs) in oysters using official method AOAC 2005.06 and 959.08 were investigated in detail with regard to possible matrix effects. Method AOAC 2005.06 gave results 2-3 times higher than the mouse bioassay method, 959.08, differences thought to be due to underestimation of PSTs by the mouse bioassay. In order to prove the cause of these large differences, work was conducted here to examine the presence and effects of matrix components on the performance of each of the two assays. A range of oyster, cockle and mussel samples were extracted using the AOAC 959.08 hydrochloric acid (HCl) extraction method and analysed for PSP by both MBA and LC-FLD. In addition, extracts were analysed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for metals as well as being subjected to a range of nutritional testing methods. Whilst there was no evidence for effect of nutritional components on either assay, ICP-MS analysis revealed a relationship between samples exhibiting the largest differences in relative method performance, specifically those with the largest LC-FLD/MBA toxicity ratio, and samples containing the highest concentrations of zinc and manganese. In order to prove the potential effect of the metals on either the LC-FLD and/or MBA assays, HCl extracts of a range of shellfish were subjected to a number of matrix modifications. Firstly, a number of PSP-positive oyster samples were processed to reduce the concentrations of metals within the extracts, without significantly reducing the concentrations of PSTs. Secondly, a range of mussel and cockle extracts, plus a standard solution of saxitoxin di-hydrochloride were spiked at variable concentrations of zinc. All treated and non-treated extracts, plus a number of controls were subjected to ICP-MS, LC-FLD and MBA testing. Results proved the absence of any effect of metals on the performance of the LC-FLD, whilst showing a large suppressive effect of the metals on the MBA. As such, the results show the performance of the official MBA is potentially unsafe for application to the routine monitoring of PSP toxicity in oysters or in any other shellfish found to contain high concentrations of metal ions.
authors.affiliation: Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. andrew.turner@cefas.co.uk
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Turner
authors.forename: Monika
authors.initials: M
authors.lastname: Dhanji-Rapkova
authors.forename: Myriam
authors.initials: M
authors.lastname: Algoet
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Suarez-Isla
authors.forename: Marco
authors.initials: M
authors.lastname: Cordova
authors.forename: Catherine
authors.initials: C
authors.lastname: Caceres
authors.forename: Cory J
authors.initials: CJ
authors.lastname: Murphy
authors.forename: Melanie
authors.initials: M
authors.lastname: Casey
authors.forename: David N
authors.initials: DN
authors.lastname: Lees
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D049873:Cardiidae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D010125:Ostreidae / Q000737:chemistry*; D015203:Reproducibility of Results; D012530:Saxitoxin / Q000032:analysis; D012758:Shellfish; D057096:Shellfish Poisoning / Q000175:diagnosis; D015032:Zinc / Q000032:analysis
chemical_list: D008387:Marine Toxins; D012530:Saxitoxin; D015032:Zinc