title: Rapid identification of YVDD mutants of hepatitis B virus using smart amplification process with competition probe.
abstract: Accurate and timely detection of drug-associated viral mutants is important during antiviral therapy. Combining Smart Amplification Process (SMAP) with competition probe, an assay specifically designed to detect point mutation at codon 204 of the hepatitis B virus (HBV) polymerase gene was developed. This assay was sensitive to detect 20 copies of mutant/reaction and recognize as little as 1% of minor mutants in the viral population. The comparison of direct sequencing and SMAP method on 35 clinical specimens showed the concordance in 88% of the cases. This method provides an efficient alternative for rapid identification of HBV mutation associated with lamivudine resistance.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Lou
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wu
authors.forename: Chen
authors.initials: C
authors.lastname: Feng
authors.forename: Nan-Ping
authors.initials: NP
authors.lastname: Wu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003062:Codon; D017931:DNA Primers / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000821:virology; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology* / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests / Q000191:economics / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D017354:Point Mutation; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D003062:Codon; D017931:DNA Primers; D004279:DNA, Viral; D019259:Lamivudine