title: Pathogen effectors target Arabidopsis EDS1 and alter its interactions with immune regulators.
abstract: Plant resistance proteins detect the presence of specific pathogen effectors and initiate effector-triggered immunity. Few immune regulators downstream of resistance proteins have been identified, none of which are known virulence targets of effectors. We show that Arabidopsis ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1), a positive regulator of basal resistance and of effector-triggered immunity specifically mediated by Toll-interleukin-1 receptor-nucleotide binding-leucine-rich repeat (TIR-NB-LRR) resistance proteins, forms protein complexes with the TIR-NB-LRR disease resistance proteins RPS4 and RPS6 and with the negative immune regulator SRFR1 at a cytoplasmic membrane. Further, the cognate bacterial effectors AvrRps4 and HopA1 disrupt these EDS1 complexes. Tight association of EDS1 with TIR-NB-LRR-mediated immunity may therefore derive mainly from being guarded by TIR-NB-LRR proteins, and activation of this branch of effector-triggered immunity may directly connect to the basal resistance signaling pathway via EDS1.
authors.affiliation: Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Saikat
authors.initials: S
authors.lastname: Bhattacharjee
authors.forename: Morgan K
authors.initials: MK
authors.lastname: Halane
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Walter
authors.initials: W
authors.lastname: Gassmann
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate; D008954:Models, Biological; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C098294:AvrRps4 protein, Pseudomonas syringae; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; D010940:Plant Proteins; C543707:RPS6 protein, Arabidopsis; C482739:SNC1 protein, Arabidopsis; C543708:SRFR1 protein, Arabidopsis; C097421:rps4 protein, plant