title: Acetylation-dependent regulation of mitochondrial ALDH2 activation by SIRT3 mediates acute ethanol-induced eNOS activation.
abstract: Moderate alcohol consumption has beneficial effects on endothelial nitric-oxide synthase (eNOS) activation, which can engender an array of anti-atherogenic actions. Here we show that in human aortic endothelial cells (HAECs), rapid activation of mitochondrial aldehyde dehydrogenase 2 (ALDH2) mediates ethanol-induced eNOS activation by preventing reactive oxygen species (ROS) accumulation. Furthermore, activation of ALDH2 by ethanol is due to its hyperacetylation by SIRT3 inactivation. These data suggest that ethanol-induced eNOS activation in HAECs may be dependent on ALDH2 hyperacetylation by SIRT3 inactivation.
authors.affiliation: Department of Emergency, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xue
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Lujing
authors.initials: L
authors.lastname: Meng
authors.forename: Shujian
authors.initials: S
authors.lastname: Wei
authors.forename: Jiali
authors.initials: J
authors.lastname: Wang
authors.forename: Panpan
authors.initials: P
authors.lastname: Hao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Bian
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D000431:Ethanol / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D056566:Sirtuin 3 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D000431:Ethanol; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C096127:ALDH2 protein, human; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000123:Acetyltransferases; C466093:SIRT3 protein, human; D056566:Sirtuin 3