title: The structure of LsrB from Yersinia pestis complexed with autoinducer-2.
abstract: The crystal structure of LsrB from Yersinia pestis complexed with autoinducer-2 (AI-2; space group P2(1)2(1)2(1), unit-cell parameters a = 40.61, b = 61.03, c = 125.23 Å) has been solved by molecular replacement using the structure of LsrB from Salmonella typhimurium (PDB entry 1tjy) and refined to R = 0.180 (R(free) = 0.213) at 1.75 Å resolution. The electron density for bound AI-2 and the stereochemistry of the AI-2-binding site are consistent with bound AI-2 adopting the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, just as has been observed in the crystal structures of the Salmonella typhimurium and Sinorhizobium meliloti LsrB-AI-2 complexes.
authors.affiliation: Microbiology Department, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Kavanaugh
authors.forename: Lokesh
authors.initials: L
authors.lastname: Gakhar
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Horswill
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry*; D017124:Conserved Sequence; D006714:Homoserine / Q000031:analogs & derivatives* / Q000737:chemistry; D007783:Lactones / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D015010:Yersinia pestis / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine