title: Engineered glucose isomerase from Streptomyces sp. SK is resistant to Ca²⁺ inhibition and Co²⁺ independent.
abstract: The role of two amino acid residues linked to the two catalytic histidines His54 and His220 in kinetics and physicochemical properties of the Streptomyces sp. SK glucose isomerase (SKGI) was investigated by site-directed mutagenesis and molecular modeling. Two single mutations, F53L and G219D, and a double mutation F53L/G219D was introduced into the xylA SKGI gene. The F53L mutation increases the thermostability and the catalytic efficiency and also slightly shifts the optimum pH from 6.5 to 7, but displays a profile being similar to that of the wild-type enzyme concerning the effect of various metal ions. The G219D mutant is resistant to calcium inhibition retaining about 80% of its residual activity in 10 mM Ca²⁺ instead of 10% for the wild-type. This variant is activated by Mn²⁺ ions, but not Co²⁺, as seen for the wild-type enzyme. It does not require the latter for its thermostability, but has its half-life time displaced from 50 to 20 min at 85°C. The double mutation F53L/G219D restores the thermostability as seen for the wild-type enzyme while maintaining the resistance to the calcium inhibition. Molecular modeling suggests that the increase in thermostability is due to new hydrophobic interactions stabilizing α2 helix and that the resistance to calcium inhibition is a result of narrowing the binding site of catalytic ion.
authors.affiliation: Laboratoire de Microorganismes et de Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour Km 6, B.P 1177, 3018 Sfax, Tunisia.
authors.forename: Hajer Ben
authors.initials: HB
authors.lastname: Hlima
authors.forename: Nushin
authors.initials: N
authors.lastname: Aghajari
authors.forename: Mamdouh Ben
authors.initials: MB
authors.lastname: Ali
authors.forename: Richard
authors.initials: R
authors.lastname: Haser
authors.forename: Samir
authors.initials: S
authors.lastname: Bejar
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism; D004795:Enzyme Stability; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D003035:Cobalt; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D002118:Calcium