title: The effect of glucocorticoids on sex steroid synthesis in cultured Taenia crassiceps Wake Forest University (WFU) cysticerci.
abstract: We have shown previously that cultured Taenia crassiceps Wake Forest University (WFU) and Taenia solium cysticerci, as well as the adult worms, synthesize sex steroid hormones from [3H]steroid precursors and that androgens and oestrogens influence the in vitro development of the parasites. Glucocorticoids (GCs) are used to control the inflammation caused by T. solium cysticerci in the brain. These steroids stimulate oestrogen synthesis in several tissues. Since there is no information on the effect of GC on the endocrine function of cysticerci, we investigated the effect of natural and synthetic GCs on the synthesis of oestrogens in cultured T. crassiceps WFU cysticerci. The cysticerci were obtained from the peritoneal cavity of infected female BALB/c mice; the cysts were washed extensively and pre-cultured in Dulbecco's Modified Eagle's Medium (DMEM) plus antibiotics for 5 days. The parasites were further cultured with different doses of corticosterone, dexamethasone or the vehicle for 5 days. [3H]Dehydroepiandrosterone (3H-DHEA) was added to the media and the cysticerci were further incubated for 6 or 24 h. Media were then removed and the steroids ether-extracted. Aliquots of the media were seeded on silica gel plates and developed in solvent systems. Parasites incubated in the presence of 3H-DHEA synthesized [3H]androstenediol, [3H]testosterone and [3H]17β-oestradiol ([3H]17β-E2). The addition of 100 nm or higher corticosterone doses to the media increased [3H]17β-E2 synthesis fourfold after 24 h. Dexamethasone also increased [3H]17β-E2 synthesis. The experiments presented here show for the first time that corticosterone and the synthetic GC dexamethasone modulate the synthesis of oestrogens by cysticerci.
authors.affiliation: Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, IPN, México, DF, México.
authors.forename: L
authors.initials: L
authors.lastname: Hinojosa
authors.forename: R A
authors.initials: RA
authors.lastname: Valdez
authors.forename: V
authors.initials: V
authors.lastname: Salvador
authors.forename: A G
authors.initials: AG
authors.lastname: Rodríguez
authors.forename: K
authors.initials: K
authors.lastname: Willms
authors.forename: M C
authors.initials: MC
authors.lastname: Romano
mesh_terms: D000818:Animals; D005260:Female; D005938:Glucocorticoids / Q000378:metabolism*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D013256:Steroids / Q000378:metabolism*; D013621:Taenia / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D005938:Glucocorticoids; D012739:Gonadal Steroid Hormones; D013256:Steroids