title: The composition of house dust mite is critical for mucosal barrier dysfunction and allergic sensitisation.
abstract: BACKGROUND
  House dust mite (HDM) allergens have been reported to increase airway epithelial permeability, thereby facilitating access of allergens and allergic sensitisation.
  
  
  OBJECTIVE
  The authors aimed to understand which biochemical properties of HDM are critical for epithelial immune and barrier responses as well as T helper 2-driven experimental asthma in vivo.
  
  
  METHODS
  Three commercially available HDM extracts were analysed for endotoxin levels, protease and chitinase activities and effects on transepithelial resistance, junctional proteins and pro-inflammatory cytokine release in the bronchial epithelial cell line 16HBE and normal human bronchial cells. Furthermore, the effects on epithelial remodelling and airway inflammation were investigated in a mouse model.
  
  
  RESULTS
  The different HDM extracts varied extensively in their biochemical properties and induced divergent responses in vitro and in vivo. Importantly, the Greer extract, with the lowest serine protease activity, induced the most pronounced effects on epithelial barrier function and CCL20 release in vitro. In vivo, this extract induced the most profound epithelial E-cadherin delocalisation and increase in CCL20, CCL17 and interleukin 5 levels, accompanied by the most pronounced induction of HDM-specific IgE, goblet cell hyperplasia, eosinophilic inflammation and airway hyper-reactivity.
  
  
  CONCLUSIONS
  This study shows the ability of HDM extracts to alter epithelial immune and barrier responses is related to allergic sensitisation but independent of serine/cysteine protease activity.
authors.affiliation: Laboratory of Allergology and Pulmonary Diseases, Department of Pathology and Medical Biology, University Medical Center Groningen, Hanzeplein 1, NL-9713GZ Groningen, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Post
authors.forename: M C
authors.initials: MC
authors.lastname: Nawijn
authors.forename: T L
authors.initials: TL
authors.lastname: Hackett
authors.forename: M
authors.initials: M
authors.lastname: Baranowska
authors.forename: R
authors.initials: R
authors.lastname: Gras
authors.forename: A J M
authors.initials: AJ
authors.lastname: van Oosterhout
authors.forename: I H
authors.initials: IH
authors.lastname: Heijink
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000276:immunology; D054414:Chemokine CCL17 / Q000276:immunology; D054418:Chemokine CCL20 / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D066298:In Vitro Techniques; D015848:Interleukin-5 / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039981:Pyroglyphidae / Q000276:immunology*; D020545:Respiratory Mucosa / Q000276:immunology* / Q000503:physiopathology
chemical_list: D039741:Antigens, Dermatophagoides; D015415:Biomarkers; C108355:CCL20 protein, human; C493447:CCL20 protein, mouse; D015820:Cadherins; C499538:Ccl17 protein, mouse; D054414:Chemokine CCL17; D054418:Chemokine CCL20; D016207:Cytokines; D015848:Interleukin-5