title: Antioxidant phenolic profile from ethyl acetate fraction of Fructus Ligustri Lucidi with protection against hydrogen peroxide-induced oxidative damage in SH-SY5Y cells.
abstract: In this study, we demonstrated the antioxidant and protective properties of crude extract and fractions from Fructus Ligustri Lucidi (FLL) against hydrogen peroxide (H2O2)-induced oxidative damage in SH-SY5Y cells. The contents of their phytochemical profiles were determined by spectrophotometric methods and high performance liquid chromatography using a photodiode array detector. FLL crude extract possessed appreciable scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl and H2O2. The ethyl acetate (EtOAc) fraction was the most active fraction in scavenging free radicals and H2O2. Following exposure of cells to H2O2, there was a marked decrease in cell survival and intracellular antioxidant enzymes, and then intracellular oxidative stress, the level of lipid peroxidation, and caspase-3 activity were increased. Simultaneous treatment with the EtOAc fraction blocked these H2O2-induced cellular events. Hydroxytyrosol and salidroside are major components of the EtOAc fraction. These results show that the phenolic-enriched EtOAc fraction of FLL contains tyrosol-related derivatives and exerts the protective effects against H2O2 toxicity via its free radical scavenging activity and ability to elevate the levels of antioxidant enzymes.
authors.affiliation: The School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, and Taichung Hospital, Department of Health, No. 91, Hsueh Shih Road, Taichung 40402, Taiwan, ROC.
authors.forename: Heng-Yin
authors.initials: HY
authors.lastname: Ju
authors.forename: Shiu Ching
authors.initials: SC
authors.lastname: Chen
authors.forename: Kuo-Jen
authors.initials: KJ
authors.lastname: Wu
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Kuo
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Hseu
authors.forename: Hui
authors.initials: H
authors.lastname: Ching
authors.forename: Chi-Rei
authors.initials: CR
authors.lastname: Wu
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation; D008516:Medicine, Chinese Traditional; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000085:Acetates; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C007650:ethyl acetate; D006861:Hydrogen Peroxide