title: MALDI mass spectrometric imaging of cardiac tissue following myocardial infarction in a rat coronary artery ligation model.
abstract: Although acute myocardial infarction (MI) is consistently among the top causes of death in the United States, the spatial distribution of lipids and metabolites following MI remains to be elucidated. This work presents the investigation of an in vivo rat model of MI using mass spectrometric imaging (MSI) and multivariate data analysis. MSI was conducted on cardiac tissue following a 24-h left anterior descending coronary artery ligation to analyze multiple compound classes. First, the spatial distribution of a small metabolite, creatine, was used to identify areas of infarcted myocardium. Second, multivariate data analysis and tandem mass spectrometry were used to identify phospholipid (PL) markers of MI. A number of lysophospholipids demonstrated increased ion signal in areas of infarction. In contrast, select intact PLs demonstrated decreased ion signal in the area of infarction. The complementary nature of these two lipid classes suggests increased activity of phospholipase A(2), an enzyme that has been implicated in coronary heart disease and inflammation.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, United States.
authors.forename: Robert F
authors.initials: RF
authors.lastname: Menger
authors.forename: Whitney L
authors.initials: WL
authors.lastname: Stutts
authors.forename: Dhanalakshmi S
authors.initials: DS
authors.lastname: Anbukumar
authors.forename: John A
authors.initials: JA
authors.lastname: Bowden
authors.forename: David A
authors.initials: DA
authors.lastname: Ford
authors.forename: Richard A
authors.initials: RA
authors.lastname: Yost
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000378:metabolism* / Q000473:pathology*; D006321:Heart / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008026:Ligation; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry