title: Immobilization-free screening of aptamers assisted by graphene oxide.
abstract: Graphene oxide (GO) has the ability to separate free short ssDNA in heterogeneous solution. This feature is applied as a label free platform for screening of aptamers that bind to their target with high affinity and specificity. Herein, we report an aptamer selection strategy for Nampt protein based on GO.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, South Korea.
authors.forename: Jee-Woong
authors.initials: JW
authors.lastname: Park
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Tatavarty
authors.forename: Dae Woo
authors.initials: DW
authors.lastname: Kim
authors.forename: Hee-Tae
authors.initials: HT
authors.lastname: Jung
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
mesh_terms: D000327:Adsorption; D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000302:isolation & purification / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D010087:Oxides / Q000737:chemistry*; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D010087:Oxides; D006108:Graphite; D054409:Nicotinamide Phosphoribosyltransferase