title: Engineering hydrophobin DewA to generate surfaces that enhance adhesion of human but not bacterial cells.
abstract: Hydrophobins are fungal proteins with the ability to form immunologically inert membranes of high stability, properties that makes them attractive candidates for orthopaedic implant coatings. Cell adhesion on the surface of such implants is necessary for better integration with the neighbouring tissue; however, hydrophobin surfaces do not mediate cell adhesion. The aim of this project was therefore to investigate whether the class I hydrophobin DewA from Aspergillus nidulans can be functionalized for use on orthopaedic implant surfaces. DewA variants bearing either one RGD sequence or the laminin globular domain LG3 binding motif were engineered. The surfaces of both variants showed significantly increased adhesion of mesenchymal stem cells (MSCs), osteoblasts, fibroblasts and chondrocytes; in contrast, the insertion of binding motifs RGD and LG3 in DewA did not increase Staphylococcus aureus adhesion to the hydrophobin surfaces. Proliferation of MSCs and their osteogenic, chondrogenic and adipogenic differentiation potential were not affected on these surfaces. The engineered surfaces therefore enhanced MSC adhesion without interfering with their functionality or leading to increased risk of bacterial infection.
authors.affiliation: Research Centre for Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, Schlierbacher Landstrasse 200a, 69118 Heidelberg, Germany.
authors.forename: Stephane
authors.initials: S
authors.lastname: Boeuf
authors.forename: Tanja
authors.initials: T
authors.lastname: Throm
authors.forename: Beatrice
authors.initials: B
authors.lastname: Gutt
authors.forename: Timo
authors.initials: T
authors.lastname: Strunk
authors.forename: Marc
authors.initials: M
authors.lastname: Hoffmann
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Seebach
authors.forename: Leonie
authors.initials: L
authors.lastname: Mühlberg
authors.forename: Jan
authors.initials: J
authors.lastname: Brocher
authors.forename: Tobias
authors.initials: T
authors.lastname: Gotterbarm
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wenzel
authors.forename: Reinhard
authors.initials: R
authors.lastname: Fischer
authors.forename: Wiltrud
authors.initials: W
authors.lastname: Richter
mesh_terms: D001233:Aspergillus nidulans / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000166:cytology / Q000254:growth & development*; D013499:Surface Properties
chemical_list: C095254:DEWA protein, Asperigillus nidulans; D005656:Fungal Proteins