title: Blood-based miRNA preparation for noninvasive biomarker development.
abstract: This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The methods described here are recently introduced protocols dedicated to the isolation of total RNAs including small RNAs, e.g., miRNeasy Kit and PAXgene Blood miRNA Kit, or alternatively for the enrichment of low-molecular-weight RNA (LMW RNA) fractions including small RNAs, e.g., using the miRNeasy Kit. Furthermore, modifications of classical RNA purification protocols to facilitate the recovery of small RNAs are highlighted.
authors.affiliation: Life and Medical Sciences (LIMES), Genomics and Immunoregulation, University of Bonn, Bonn, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Debey-Pascher
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Thorsten
authors.initials: T
authors.lastname: Voss
authors.forename: Andrea
authors.initials: A
authors.lastname: Staratschek-Jox
mesh_terms: D015415:Biomarkers / Q000097:blood; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D035683:MicroRNAs / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D011786:Quality Control; D012313:RNA / Q000032:analysis / Q000097:blood / Q000302:isolation & purification
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012313:RNA