title: p53 codon 72 polymorphisms and random amplified polymorphic DNA analysis             of non-melanoma skin cancer through archival formalin-fixed paraffin-embedded             tissue.
abstract: Non-melanoma skin cancer (NMSC) is classified among the ten most frequent             cancers in Malaysia. A common polymorphism at codon 72 of the p53 tumor suppressor             gene and its influence on cancer risk has been studied for different types of             cancer with mixed and inconsistent results with limited published data on the             Malaysian population so far. In the present study, the frequency of p53 codon             72 polymorphism in 60 patients with NMSC was investigated from archival formalin-fixed             paraffin-embedded (FFPE) tissue obtained from Hospital Universiti Kebangsaan Malaysia             (HUKM). Additionally, random amplified polymorhic DNA -polymorphic chain reaction             (RAPD-PCR) was employed for preliminary biomarker development. NMSC FFPE samples             (70%) possess Arg/Arg, 20% with Pro/Pro and 10% with Arg/Pro. In total, there             was no significant difference in the p53 codon 72 genotypes between histological             types of NMSC, gender, race, tumor location and age group. However, there was             an apparent age-associated increase in the Arg/Arg genotype but did not reach             statistical significance (P=0.235). NMSC types and demographic characteristics             did not influence genotype distribution. On the other hand, BCC and SCC distributions             are influenced by age group, race and tumor location.
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia. ykcheah@medic.upm.edu.my
authors.forename: Cheah
authors.initials: C
authors.lastname: Yoke-Kqueen
authors.forename: Nurul-Syakima
authors.initials: NS
authors.lastname: Ab Mutalib
authors.forename: Shiran Mohd
authors.initials: SM
authors.lastname: Sidik
authors.forename: Lee
authors.initials: L
authors.lastname: Learn-Han
authors.forename: Tan
authors.initials: T
authors.lastname: Geok-Chin
mesh_terms: D000328:Adult; D004252:DNA Mutational Analysis; D005260:Female; D005557:Formaldehyde; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008296:Malaysia; D008297:Male; D008545:Melanoma / Q000235:genetics; D008875:Middle Aged; D016612:Paraffin Embedding; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D005557:Formaldehyde