title: Age- and sex-associated plasma proteomic changes in growth hormone receptor gene-disrupted mice.
abstract: Growth hormone receptor gene-disrupted (GHR-/-) mice are dwarf, insulin sensitive, and long lived despite being obese. In order to identify characteristics associated with their increased longevity, we studied age-related plasma proteomic changes in these mice. Male and female GHR-/- mice and their littermate controls were followed longitudinally at 8, 16, and 24 months of ages for plasma proteomic analysis. Relative to control littermates, GHR-/- mice had increased levels of apolipoprotein A-4 and retinol-binding protein-4 and decreased levels of apolipoprotein E, haptoglobin, and mannose-binding protein-C. Female GHR-/- mice showed decreased inflammatory cytokines including interleukin-1β and monocyte chemotactic protein-1. Additionally, sex differences were found in specific isoforms of apolipoprotein E, RBP-4, haptoglobin, albumin, and hemoglobin subunit beta. In conclusion, we find plasma proteomic changes in GHR-/- mice that favor a longer life span as well as sex differences indicative of an improved health span in female mice.
authors.affiliation: Edison Biotechnology Institute, 1 Water Tower Drive, The Ridges, Ohio University, Athens, OH 45701, USA.
authors.forename: Juan
authors.initials: J
authors.lastname: Ding
authors.forename: Darlene E
authors.initials: DE
authors.lastname: Berryman
authors.forename: Adam
authors.initials: A
authors.lastname: Jara
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
mesh_terms: D000367:Age Factors; D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism*; D001823:Body Composition; D002352:Carrier Proteins / Q000235:genetics; D016207:Cytokines / Q000097:blood; D005260:Female; D008136:Longevity / Q000502:physiology*; D008297:Male; D051379:Mice; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D011986:Receptors, Somatotropin / Q000235:genetics*; D054839:Retinol-Binding Proteins, Plasma / Q000378:metabolism; D012737:Sex Factors
chemical_list: D001054:Apolipoproteins A; D002352:Carrier Proteins; D016207:Cytokines; C502487:Rbp4 protein, mouse; D011986:Receptors, Somatotropin; D054839:Retinol-Binding Proteins, Plasma; C022205:apolipoprotein A-IV; C051815:somatotropin-binding protein