title: Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: deletion of gal7.
abstract: MIRAGE is a unique in vivo genome editing technique that exploits the inherent instability of inverted repeats (palindromes) in the Saccharomyces cerevisiae chromosome. As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications that require creation of designer strains of this yeast. In particular, it has various potential applications in metabolic engineering, systems biology, synthetic biology, and molecular genetics.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Nikhil U
authors.initials: NU
authors.lastname: Nair
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D017353:Gene Deletion; D023061:Gene Order; D005818:Genetic Engineering / Q000379:methods*; D059765:Homologous Recombination; D055029:Inverted Repeat Sequences; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C554167:URA3 protein, S cerevisiae