title: Enantioselective determination of triazole fungicide tebuconazole in vegetables, fruits, soil and water by chiral liquid chromatography/tandem mass spectrometry.
abstract: A novel and sensitive method was developed for the determination of tebuconazole enantioselectively using reversed-phase LC-MS/MS. The separation and determination were performed using on an amylose-based chiral stationary phase, a Lux 3u Amylose-2 column (150 mm×2.0 mm), under isocratic conditions at 0.3 mL/min flow rate. A series of chiral stationary phases were investigated and the effect of mobile phase composition on the enantioseparation was discussed. Parameters including the matrix effect, linearity, precision, accuracy and stability were evaluated. Under optimal conditions, the overall mean recoveries for two enantiomers from the soil, tomato, cucumber, pear and apple samples were 79.3-101.1% with 2.8-11.5% intra-day relative standard deviations (RSDs) and 4.1-8.6% inter-day RSDs at 5, 25 and 50 μg/kg levels; the mean enantiomer recoveries from the water samples were 89.6-101.9% with 3.3-10.2% intra-day RSDs and 5.1-7.7% inter-day RSDs at 0.25, 0.5 and 2.5 μg/kg levels. The limits of detection (LODs) for all enantiomers in tomato, cucumber, pear, apple, soil and water were less than 0.6 μg/kg, whereas the limit of quantification (LOQ) did not exceed 2.0 μg/kg. The results indicate that this proposed method is convenient and reliable for the enantioselective determination of tebuconazole enantiomers in foods and environment samples.
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Beijing, P. R. China. yongquan_zheng@yahoo.com.cn
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Kong
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D005659:Fungicides, Industrial / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D012989:Soil Pollutants / Q000032:analysis; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014230:Triazoles / Q000032:analysis* / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D005659:Fungicides, Industrial; D012989:Soil Pollutants; D014230:Triazoles; D014874:Water Pollutants, Chemical; C087114:tebuconazole