title: Homeostatic changes of short-term plasticity of gabaergic synaptic transmission in rat hippocampal cell cultures.
abstract: It is well documented that prolonged alteration of activity in neuronal networks initiates a number of homeostatic mechanisms including compensatory changes of excitatory and inhibitory synaptic strength. We studied whether this also evokes compensatory changes of short-term synaptic transmission. Using patch-clamp technique in hippocampal cell cultures we examined the effects: of prolonged decrease of neuronal firing evoked by sodium channel blocker: tetrodotoxin (TTX) and ionotropic glutamate receptor antagonist - kynurenate; prolonged enhancement ofneuronal firing evoked by antagonist GABAA receptors - bicuculline on short-term depression of GABAergic synaptic transmission evoked by train of stimuli (5 Hz). We found that both TTX and kynurenate treatments enhance depression of GABAergic transmission, while bicuculline treatment does not. We conclude that alteration of depression of GABAergic transmission evoked by the prolonged decrease of neuronal activity may contribute to homeostatic plasticity in hippocampal neuronal networks.
authors.affiliation: A.A. Bogomoletz Institute of Physiology, National Academy of Sciences, Kiev, Ukraine. maksim@biph.kiev.ua
authors.forename: S Y
authors.initials: SY
authors.lastname: Ivanova
authors.forename: M V
authors.initials: MV
authors.lastname: Storozhuk
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D059330:GABAergic Neurons / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D007736:Kynurenic Acid / Q000494:pharmacology; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D026941:Sodium Channel Blockers / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D007736:Kynurenic Acid