title: Evaluation of cell death after treatment with extracorporeal photopheresis.
abstract: The aim of our study is to assess the mortality of leukocytes during extracorporeal photopheresis. Sixty-three photopheresis performed on 13 patients affected by chronic GvHD were evaluated. Samples were analyzed using a FACSCalibur flow cytometer. Apoptosis and necrosis of limphomononuclear cells dramatically increased after the apheretic procedure. We found a further increase of apoptotic and necrotic limphomononuclear cells after treatment with 8-MOP and UVA (p≤0.05). Our data suggested that the immunomodulatory effects of extracorporeal photopheresis, triggered by circulating apoptotic or necrotic cells, could play an important role in the treatment of GvHD with this procedure.
authors.affiliation: Immunohematology Section, Tor Vergata University, Rome, Italy. daniele_nicola2000@yahoo.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Daniele
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Del Proposto
authors.forename: Paola
authors.initials: P
authors.lastname: Cerrone
authors.forename: Silvia
authors.initials: S
authors.lastname: Sinopoli
authors.forename: Lucia
authors.initials: L
authors.lastname: Sansone
authors.forename: Deborah Ilaria
authors.initials: DI
authors.lastname: Gadaleta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lanti
authors.forename: Angelo Salvatore
authors.initials: AS
authors.lastname: Ferraro
authors.forename: Stefano
authors.initials: S
authors.lastname: Spurio
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Scerpa
authors.forename: Francesco
authors.initials: F
authors.lastname: Zinno
authors.forename: Gaspare
authors.initials: G
authors.lastname: Adorno
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Isacchi
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D005260:Female; D006086:Graft vs Host Disease / Q000097:blood / Q000188:drug therapy* / Q000473:pathology*; D006801:Humans; D007962:Leukocytes / Q000473:pathology*; D008297:Male; D008730:Methoxsalen / Q000008:administration & dosage*; D008875:Middle Aged; D009336:Necrosis / Q000097:blood / Q000473:pathology; D017893:Photopheresis / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage*
chemical_list: D017319:Photosensitizing Agents; D008730:Methoxsalen