title: Changes in morphology and activity of transglutaminase following cross-linking and immobilization on a polypropylene microporous membrane.
abstract: Transglutaminase (TGase) was cross-linked with glutaraldehyde, and cross-linked crystalline transglutaminase was immobilized on a polypropylene microporous membrane by UV-induced grafting. Immobilized enzyme activity were calculated to be 0.128 U/cm² polypropylene microporous membrane. The microstructure and enzyme characteristics of free, cross-linked and immobilized transglutaminase were compared. The optimum temperature of free transglutaminase was determined to be approximately 40 °C, while cross-linking and immobilization resulted in an increase to approximately 45 °C and 50 °C. At 60 °C, immobilized, cross-linked and free transglutaminase retained 91.7 ± 1.20%, 63.2 ± 1.05% and 37.9 ± 0.98% maximum activity, respectively. The optimum pH was unaffected by the state of transglutaminase. However, the thermal and pH stabilities of cross-linked and immobilized transglutaminase were shown to increase.
authors.affiliation: Key Laboratory of Food Science and Engineering of Heilongjiang Province, Harbin University of Commerce, Harbin 150076, China.
authors.forename: Yan-Guo
authors.initials: YG
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Qian
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Chun-Ran
authors.initials: CR
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Zhang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Ma
mesh_terms: D003432:Cross-Linking Reagents / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism* / Q000648:ultrastructure*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D011126:Polypropylenes / Q000737:chemistry*; D016062:Porosity; D013696:Temperature; D011503:Transglutaminases / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011126:Polypropylenes; D011503:Transglutaminases