title: Short-term in-vitro culture of goat enriched spermatogonial stem cells using different serum concentrations.
abstract: OBJECTIVE
  To investigate the effect of serum supplementing on short-term culture, fate determination and gene expression of goat spermatogonial stem cells (SSCs).
  
  
  METHODS
  Crude testicular cells were plated over Datura-Stramonium Agglutinin (DSA) for 1 h, and non-adhering cells were cultured in the presence of different serum concentrations (1, 5, 10, and 15%) for 7 days in a highly enriched medium initially developed in mice. Colonies developed in each group were used for the assessment of morphology, immunocytochemistry, and gene expression.
  
  
  RESULTS
  Brief incubation of testicular cells with DSA resulted in a significant increase in the number of cells that expressed the germ cell marker (VASA). The expression of THY1, a specific marker of undifferentiated spermatogonia, was significantly higher in colonies developed in the presence of 1% rather than 5, 10 and 15% serum.
  
  
  CONCLUSIONS
  Goat SSCs could proliferate and maintain in SSC culture media for 1 week at serum concentrations as low as 1%, while higher concentrations had detrimental effects on SSC culture/expansion.
authors.affiliation: Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Bahadorani
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseini
authors.forename: P
authors.initials: P
authors.lastname: Abedi
authors.forename: M
authors.initials: M
authors.lastname: Hajian
authors.forename: S E
authors.initials: SE
authors.lastname: Hosseini
authors.forename: A
authors.initials: A
authors.lastname: Vahdati
authors.forename: H
authors.initials: H
authors.lastname: Baharvand
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000373:Agglutinins / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D003470:Culture Media / Q000737:chemistry*; D013286:Datura stramonium / Q000737:chemistry; D015870:Gene Expression; D006041:Goats / Q000235:genetics*; D008297:Male; D051379:Mice; D044967:Serum / Q000737:chemistry; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D000373:Agglutinins; D003470:Culture Media; D018800:Thy-1 Antigens