title: Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy.
abstract: BACKGROUND
  Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cell cycle and cell division mechanisms. However, 3D imaging of cell division in living spheroids is technically challenging and has never been reported.
  
  
  RESULTS
  Here, we report a major breakthrough based on the engineering of multicellular tumor spheroids expressing an histone H2B fluorescent nuclear reporter protein, and specifically designed sample holders to monitor live cell division dynamics in 3D large spheroids using an home-made selective-plane illumination microscope.
  
  
  CONCLUSIONS
  As illustrated using the antimitotic drug, paclitaxel, this technological advance paves the way for studies of the dynamics of cell divion processes in 3D and more generally for the investigation of tumor cell population biology in integrated system as the spheroid model.
authors.affiliation: Université de Toulouse; ITAV-UMS3039, F-31106 Toulouse, France. bernard.ducommun@itav-recherche.fr.
authors.forename: Corinne
authors.initials: C
authors.lastname: Lorenzo
authors.forename: Céline
authors.initials: C
authors.lastname: Frongia
authors.forename: Raphaël
authors.initials: R
authors.lastname: Jorand
authors.forename: Jérôme
authors.initials: J
authors.lastname: Fehrenbach
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Amina
authors.initials: A
authors.lastname: Maandhui
authors.forename: Guillaume
authors.initials: G
authors.lastname: Gay
authors.forename: Bernard
authors.initials: B
authors.lastname: Ducommun
authors.forename: Valérie
authors.initials: V
authors.lastname: Lobjois