title: Honeycomb micropatterning of proteins on polymer films through the inverse microemulsion approach.
abstract: Here we report the rapid and convenient patterning of proteins on porous polymer film using the inverse microemulsion approach. Following this method, proteins, which were dissolved in water, were transferred into dichloromethane solution of polymers through the formation of inverse microemulsion by mixing the two solutions. The protein-containing microemulsion droplets accumulated automatically into large and stable ones on the surface of organic solution casting on solid substrates, and formed tightly packed microemulsion droplet arrays driven by surface tension. With the evaporation of organic solvent and water, the microemulsion droplet arrays, which act as the template, turn to honeycomb patterned pores bearing proteins in them. The formed protein patterns can be locally applied for the detection of other proteins through specific recognition. The generality and reproducibility for the formation of BSA/PS microporous film and protein patterning by using different polymers and solvents were demonstrated by investigating surfactant addition, polymer and solvent types, and casting volume on the morphology of the microporous films. A preliminary mechanism for the protein patterning is discussed based on the analysis of the experimental results.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Liang
authors.forename: Hang
authors.initials: H
authors.lastname: Sun
authors.forename: Lixin
authors.initials: L
authors.lastname: Wu
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Dang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D002417:Cattle; D004655:Emulsions / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D040081:Protein Array Analysis / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004655:Emulsions; D055767:Immobilized Proteins; D011137:Polystyrenes; D012710:Serum Albumin, Bovine