title: The Shaggy Inc Cap medicinal mushroom, Coprinus comatus (O.F.Mull.: Fr.) Pers. (Agaricomycetideae) substances interfere with H2O2 induction of the NF-kappaB pathway through inhibition of Ikappaalpha phosphorylation in MCF7 breast cancer cells.
abstract: Breast cancer is the most commonly diagnosed cancer among women. Currently, there is no effective therapy for malignant estrogen-independent breast cancer. In our study, we used hydrogen peroxide, a well-known strong oxidative reagent capable of activating the nuclear factor kappa B (NF-kappaB) transcription factor. The IC50 value of the culinary-medicinal Shaggy Inc Cap mushroom Coprinus comatus culture liquid crude extract on MCF7 cell viability was found to be as low as 76 microg/mL, and the IC50 value of C. comatus ethyl acetate extract was only 32 microg/ mL. Our results also showed that both extracts significantly affected IkappaBalpha phosphorylation in a dose-dependent manner. The effect of ethyl acetate extract was comparable to the effect of curcumin, a known NF-kappaB pathway inhibitor, and seemed to be the most active inhibitor of H2O2-dependent IkappaBalpha phosphorylation. In addition, the data obtained showed that only ethyl acetate extract inhibited the activity of IKK complex, at close to 90% as compared to the control of the untreated sample. These results suggest that C. comatus contains potent compounds capable of inhibiting NF-kappaB function and also possibly acts as an antitumor agent.
authors.affiliation: Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences & Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel.
authors.forename: Mikheil D
authors.initials: MD
authors.lastname: Asatiani
authors.forename: Solomon P
authors.initials: SP
authors.lastname: Wasser
authors.forename: Eviatar
authors.initials: E
authors.lastname: Nevo
authors.forename: Nili
authors.initials: N
authors.lastname: Ruimi
authors.forename: Jamal
authors.initials: J
authors.lastname: Mahajna
authors.forename: Abraham Z
authors.initials: AZ
authors.lastname: Reznick
mesh_terms: D000085:Acetates / Q000737:chemistry; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D003302:Coprinus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D051550:I-kappa B Kinase / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D020672:I-kappa B Proteins / Q000187:drug effects* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000085:Acetates; D045424:Complex Mixtures; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; C007650:ethyl acetate; D006861:Hydrogen Peroxide; D051550:I-kappa B Kinase