title: Stepwise motion of a microcantilever driven by the hydrolysis of viral ATPases.
abstract: The biomolecular machines involved in DNA packaging by viruses generate one of the highest mechanical powers observed in nature. One component of the DNA packaging machinery, called the terminase, has been proposed as the molecular motor that converts chemical energy from ATP hydrolysis into mechanical movement of DNA during bacteriophage morphogenesis. However, the conformational changes involved in this energy conversion have never been observed. Here we report a real-time measurement of ATP-induced conformational changes in the terminase of bacteriophage T7 (gp19). The recording of the cantilever bending during its functionalization shows the existence of a gp19 monolayer arrangement confirmed by atomic force microscopy of the immobilized proteins. The ATP hydrolysis of the gp19 terminase generates a stepped motion of the cantilever and points to a mechanical cooperative effect among gp19 oligomers. Furthermore, the effect of ATP can be counteracted by non-hydrolyzable nucleotide analogs.
authors.affiliation: Instituto de Microelectrónica de Madrid (IMM-CNM), CSIC, Madrid, Spain. jmertens@imm.cnm.csic.es
authors.forename: Johann
authors.initials: J
authors.lastname: Mertens
authors.forename: María I
authors.initials: MI
authors.lastname: Daudén
authors.forename: José L
authors.initials: JL
authors.lastname: Carrascosa
authors.forename: Javier
authors.initials: J
authors.lastname: Tamayo
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D017123:Bacteriophage T7 / Q000737:chemistry / Q000201:enzymology*; D042003:DNA Packaging; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D018625:Microscopy, Atomic Force; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D014764:Viral Proteins; D000255:Adenosine Triphosphate; D004706:Endodeoxyribonucleases; C030894:terminase; D000251:Adenosine Triphosphatases