title: Stereoselective bioreduction of 1-(4-methoxyphenyl)ethanone by whole cells of marine-derived fungi.
abstract: Nine strains of marine-derived fungi (Aspergillus sydowii Ce15, A. sydowii Ce19, Aspergillus sclerotiorum CBMAI 849, Bionectria sp. Ce5, Beauveria felina CBMAI 738, Cladosporium cladosporioides CBMAI 857, Mucor racemosus CBMAI 847, Penicillium citrinum CBMAI 1186, and Penicillium miczynskii Gc5) were screened, catalyzing the asymmetric bioreduction of 1-(4-methoxyphenyl)ethanone 1 to its corresponding 1-(4-methoxyphenyl)ethanol 2. A. sydowii Ce15 and Bionectria sp. Ce5 produced the enantiopure (R)-alcohol 2 (>99% ee) in accordance with the anti-Prelog rule and, the fungi B. felina CBMAI 738 (>99% ee) and P. citrinum CBMAI 1186 (69% ee) in accordance with the Prelog rule. Stereoselective bioreduction by whole cells of marine-derived fungi described by us is important for the production of new reductases from marine-derived fungi.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense, 400, CEP 13560-970, CP 780, São Carlos, São Paulo, Brazil.
authors.forename: Lenilson C
authors.initials: LC
authors.lastname: Rocha
authors.forename: Hercules V
authors.initials: HV
authors.lastname: Ferreira
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Luiz
authors.forename: Lara D
authors.initials: LD
authors.lastname: Sette
authors.forename: André L M
authors.initials: AL
authors.lastname: Porto
mesh_terms: D000098:Acetophenones / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000145:classification* / Q000378:metabolism*; D012623:Seawater; D013045:Species Specificity; D013237:Stereoisomerism; D014871:Water Microbiology
chemical_list: C570286:1-(5-bromo-2-hydroxy-4-methoxyphenyl)ethanone; D000098:Acetophenones