title: Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19.
abstract: Retroviral vectors have been employed in clinical trials for gene therapy owing to their relative large packaging capacity, alterable cell tropism, and chromosomal integration for stable transgene expression. However, uncontrollable integrations of transgenes are likely to cause safety issues, such as insertional mutagenesis. A targeted transgene integration system for retroviral vectors, therefore, is a straightforward way to address the insertional mutagenesis issue. Adeno-associated virus (AAV) is the only known virus capable of targeted integration in human cells. In the presence of AAV Rep proteins, plasmids possessing the p5 integration efficiency element (p5IEE) can be integrated into the AAV integration site (AAVS1) in the human genome. In this report, we describe a system that can target the circular DNA derived from non-integrating retroviral vectors to the AAVS1 site by utilizing the Rep/p5IEE integration mechanism. Our results showed that after G418 selection 30% of collected clones had retroviral DNA targeted at the AAVS1 site.
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, Nishi-ku, Fukuoka, Japan.
authors.forename: Shuohao
authors.initials: S
authors.lastname: Huang
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D019426:Integrases / Q000235:genetics*; D016254:Mutagenesis, Insertional; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004270:DNA, Circular; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C068734:rep proteins, Adeno-associated virus 2; D019426:Integrases