title: Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1.
abstract: Potato virus Y (PVY) is the most important viral pathogen of cultivated potato (Solanum tuberosum) from a commercial perspective, causing severe losses in both tuber quality and yield worldwide. Specific accessions of wild potato species exhibit resistance against PVY but efforts to transfer the trait to cultivated material have not yielded widely adopted varieties. Because amino acid substitutions at specific domains of host factor eIF4E-1 often confer resistance to various crops, we sequenced the associated genes expressed in wild potato plants. A novel eIF4E-1 variant, designated here as Eva1, was identified in S.                         chacoense, S. demissum, and S. etuberosum. The protein contains amino acid substitutions at ten different positions when compared to its cultivated potato (S. tuberosum) homolog. In the yeast two-hybrid system, Eva1 failed to bind VPg, a viral protein required for infectivity. Overexpression of the associated cDNA conferred PVY resistance to transgenic potato plants silenced for the native eIF4E-1 gene. Because the gene sources of Eva1 are sexually compatible with potato, the molecular strategies described can be employed to develop 'intragenic' potato cultivars.
authors.affiliation: Simplot Plant Sciences, JR Simplot Company, Boise, ID 83706, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Duan
authors.forename: Craig
authors.initials: C
authors.lastname: Richael
authors.forename: Caius M
authors.initials: CM
authors.lastname: Rommens
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002212:Capsicum / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D060467:Disease Resistance; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000276:immunology / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000821:virology; D017800:Potyvirus / Q000276:immunology / Q000472:pathogenicity*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D032322:Solanum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D012261:Ribonucleoproteins; D017361:Viral Nonstructural Proteins; C102206:genome-linked viral protein, potato virus Y