title: Preparation of Black Hoof medicinal mushroom Phellinus linteus (Berk. et M.A. Curt.) Teng (Aphyllophoromycetideae) beta-glucan sulfate and in vitro tumor cell growth inhibitory activity.
abstract: Polysaccharide beta-glucans were extracted from the medicinal mushroom Phellinus linteus (Hymenochaetaceae, Aphyllophoromycetideae) and subjected to sulfation. Chemical modification of the beta-glucan was confirmed by structural analysis, and its biological properties were compared with those of native beta-glucan. The results of Fourier transform infrared spectroscopy and elemental analysis indicated that successive preparation of the sulfated derivative yielded a degree of substitution of 0.47. Nitric oxide production measured by the bronchoalveolar lavage (BAL) experiments increased 1.5-fold after sulfation. In addition, the introduction of sulfate groups into the beta-glucan chains improved in vitro growth inhibitory activity against SNU-C2A cells. Therefore, sulfated beta-glucan extracted from Ph. linteus may be beneficial for immune support due to its incorporation of functional groups into its polymer structure.
authors.affiliation: Department of Food and Nutrition, Hanyang University, Seoul, Korea.
authors.forename: In Young
authors.initials: IY
authors.lastname: Bae
authors.forename: Ji-Yoon
authors.initials: JY
authors.lastname: Shin
authors.forename: Hyeon Gyu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000276:immunology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis; D000084682:Phellinus; D010936:Plant Extracts; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D013431:Sulfates / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C526145:Phellinus linteus extract; D010936:Plant Extracts; D011134:Polysaccharides; D013431:Sulfates; D047071:beta-Glucans; D009569:Nitric Oxide