title: Polioviral receptor binding ligand: a novel and safe peptide drug carrier from polioviral capsid.
abstract: Cellular uptake enhancement of green fluorescent protein (GFP) into human colon adenocarcinoma (HT-29) and human mouth epidermal carcinoma (KB) cells by a segment of VP1-BC loop polioviral capsid (V), a polioviral receptor binding peptide and HIV-I transactivator of transcription (Tat) was evaluated. HT-29 and KB cells were incubated with various molar concentrations of GFP, V, and Tat mixtures. Both V and Tat showed potent enhancement of GFP uptake into HT-29 and KB cells. In HT-29 cells, the V-GFP, Tat-GFP, and V-Tat-GFP mixtures enhanced the GFP cellular uptake efficiency with the maximum of 3.98-, 4.59-, and 4.08-folds of GFP at 1:3, 1:1/6, and 1/6:1:1/6 molar ratios, respectively. For KB cells, the V-GFP, Tat-GFP, and V-Tat-GFP mixtures enhanced the GFP cellular uptake efficiency with the maximum of 4.05-, 5.09-, and 4.91-folds of GFP at 1:1/6, 1:1, and 1:1:1 molar ratios, respectively. Both V and V-GFP mixtures showed a lower cytotoxicity effect than Tat and Tat-GFP mixture. These studies demonstrated the potential of polioviral capsid, a polioviral receptor binding peptide as a novel, low cytotoxicity carrier for the development of peptide drugs delivery system.
authors.affiliation: Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. pmpti006@chiangmai.ac.th
authors.forename: Jiradej
authors.initials: J
authors.lastname: Manosroi
authors.forename: Warangkana
authors.initials: W
authors.lastname: Lohcharoenkal
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
authors.forename: Rolf G
authors.initials: RG
authors.lastname: Werner
authors.forename: Worapaka
authors.initials: W
authors.lastname: Manosroi
authors.forename: Aranya
authors.initials: A
authors.lastname: Manosroi
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004337:Drug Carriers / Q000008:administration & dosage* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007624:KB Cells; D008024:Ligands; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D004337:Drug Carriers; D008024:Ligands; D010446:Peptide Fragments; D011991:Receptors, Virus; C442540:VP1 protein, Poliovirus; C066100:poliovirus receptor; D049452:Green Fluorescent Proteins