title: Effect of Chinese drugs for activating blood circulation and detoxifying on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome.
abstract: OBJECTIVE
  To observe the regulatory effect of Chinese drugs for activating blood circulation (ABC) and for activating blood circulation and detoxifying (ABCD) on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome.
  
  
  METHODS
  Fifty-four rabbits were randomized into the normal control group, model group, simvastatin group (simvastatin, 0.93 mg/kg per day), ABC group [Xiongshao Capsule, 0.07 g/kg per day], and ABCD group [Xiongshao Capsule, 0.07 g/kg per day, and Huanglian Capsule, 0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high-fat diets, a dose of endoxitin was given by ear vein injection, and a randomized 2-week treatment was initiated. At the end of treatment, blood lipids, circulating endothelial cells, and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases (MMP-9), tissue inhibitors to metalloproteinase (TIMP-1), granule membrane protein-140 (GMP-140), plasminogen activator inhibitor-1 (PAI-1), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and tumor necrosis factor-α(TNF-α) were determined.
  
  
  RESULTS
  Compared with the model group, ABCD group showed decreased serum triglyceride (TG) level, improvement in the pathological change in the aortic arch, and reduction in the number of circulating endothelial cells (4.00 ± 1.41 per 0.9 μL for ABCD group vs 7.83 ± 1.72 per 0.9 μL for the model group). In addition, the levels of serum GMP-140, PAI-1, and IL-6 in ABCD group were also significantly reduced [0.79 ± 0.20 ng/mL, 5.23 ± 1.39 ng/mL, 40.64 ± 10.11 pg/mL for ABCD group vs 1.08 ± 0.31 ng/mL, 7.28 ± 2.01 ng/mL, 54.44 ± 13.56 pg/mL for the model group, respectively, P < 0.05]. A trend showing improvement in the indices of thrombosis, inflammatory reaction, and tissue damage was observed in the ABC group when compared to the model group, but the changes were not statistically significant (P > 0.05).
  
  
  CONCLUSIONS
  Chinese drugs for activating blood circulation and detoxifying have beneficial effects on regulating indices of thrombosis (GMP-140 and PAI-1) and inflammatory reaction (IL-6) in rabbit model with toxic-heat and blood stasis. The effect of the activating blood circulation and detoxifying drugs in regulating the levels of serum GMP-140, PAI-1, and IL-6 was superior to that of the activating blood circulation drugs.
authors.affiliation: Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Yin
authors.forename: Cai-Feng
authors.initials: CF
authors.lastname: Wu
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Ma
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Guo
authors.forename: Ye
authors.initials: Y
authors.lastname: Huang
authors.forename: Da-Zhuo
authors.initials: DZ
authors.lastname: Shi
authors.forename: Ke-Ji
authors.initials: KJ
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D050197:Atherosclerosis / Q000188:drug therapy / Q000473:pathology; D001775:Blood Circulation / Q000187:drug effects*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012680:Sensitivity and Specificity; D019821:Simvastatin / Q000008:administration & dosage*; D018746:Systemic Inflammatory Response Syndrome / Q000188:drug therapy* / Q000473:pathology; D013927:Thrombosis / Q000188:drug therapy* / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; C494417:huanglian; C528128:xiongshao; D019821:Simvastatin