title: Nano-intercalated organophosphorus-hydrolyzing enzymes in organophosphorus antagonism.
abstract: A dendritic poly(2-alkyloxazoline)-based polymer was studied as a new carrier system for the organophosphorus-hydrolyzing recombinant enzymes, organophosphorus acid anhydrolase and organophosphorus hydrolase. Paraoxon (PO) and diisopropylfluorophosphate (DFP) were used as model organophosphorus compounds. Changes in plasma cholinesterase activity were monitored. The cholinesterase activity was proportional to the concentrations of DFP or PO. Plasma cholinesterase activity was higher in animals receiving enzyme and oxime before the organophosphates than in the oxime-only pretreated groups. These studies suggest that cholinesterase activity can serve as an indicator for the in vivo protection by the nano-intercalated organophosphorus acid anhydrolase or organophosphorus hydrolase against organophosphorus intoxications. These studies represent a practical application of polymeric nano-delivery systems as enzyme carriers in drug antidotal therapy.
authors.affiliation: Department of Chemistry, Sam Houston State University, Huntsville, Texas 77341, USA. ixp004@shsu.edu
authors.forename: Ilona
authors.initials: I
authors.lastname: Petrikovics
authors.forename: Melinda
authors.initials: M
authors.lastname: Wales
authors.forename: Marianna
authors.initials: M
authors.lastname: Budai
authors.forename: Jorn C C
authors.initials: JC
authors.lastname: Yu
authors.forename: Mária
authors.initials: M
authors.lastname: Szilasi
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D043303:Aryldialkylphosphatase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000378:metabolism; D002801:Cholinesterase Reactivators / Q000008:administration & dosage / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology / Q000379:methods*; D009943:Organophosphorus Compounds / Q000008:administration & dosage / Q000378:metabolism; D010261:Paraoxon / Q000008:administration & dosage / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D009943:Organophosphorus Compounds; D000110:Acetylcholinesterase; D043303:Aryldialkylphosphatase; D010261:Paraoxon