title: In vitro Enterococcus faecalis biofilm formation on five adhesive systems.
abstract: OBJECTIVE
  To determine the E. faecalis biofilm formation on the surface of five adhesive systems (AS) and its relationship with roughness.
  
  
  METHODS
  The formation of E. faecalis biofilms was tested on the surface of four dual-cure AS: AdheSE DC, Clearfil DC Bond, Futurabond DC and Excite DSC and one light-cure antimicrobial AS, Clearfil Protect Bond, after 24 hours of incubation, using the MBEC high-throughput device.
  
  
  RESULTS
  E. faecalis biofilms grew on all the adhesives. The least growth of biofilm was on Excite DSC, Clearfil Protect Bond, and the control. Futurabond DC resulted in the greatest roughness and biofilm amount. There was a close relationship between the quantity of biofilm and roughness, except for Clearfil Protect Bond, which showed little biofilm but high roughness.
  
  
  CONCLUSIONS
  None of the tested AS prevented E. faecalis biofilm formation, although the least quantity was found on the surface of Clearfil Protect Bond.
authors.affiliation: Department of Preventive and Operative Dentistry, School of Dentistry. University of Granada, Granada, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Baca
authors.forename: Márcia
authors.initials: M
authors.lastname: Furtado-Antunes de Freitas
authors.forename: Carmen-María
authors.initials: CM
authors.lastname: Ferrer-Luque
authors.forename: María-Paloma
authors.initials: MP
authors.lastname: González-Rodríguez
authors.forename: María-Teresa
authors.initials: MT
authors.lastname: Arias-Moliz
mesh_terms: D018441:Biofilms; D003738:Dental Cements; D013293:Enterococcus faecalis / Q000302:isolation & purification* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D003738:Dental Cements