title: Using the scan-x Web site to predict protein post-translational modifications.
abstract: The recent plethora of proteomic mass spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post-translational modification. We describe a protocol to use the scan-x Web site to view predicted acetylation sites in the human proteome and predicted phosphorylation sites in the human, mouse, fly, and yeast proteomes with high specificity. This tool is accessible from virtually any computer with a Web browser. The only requirement is a means of searching for a protein of interest in one of the represented organisms.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Chou
authors.affiliation: Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schwartz
mesh_terms: D000107:Acetylation; D000818:Animals; D004175:Diptera; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism; D020407:Internet; D008745:Methylation; D051379:Mice; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D012440:Saccharomyces; D012984:Software
chemical_list: D005656:Fungal Proteins; D019476:Insect Proteins; D011506:Proteins; D020543:Proteome