title: Efficacy of the Trichophyton ajelloi and Lagenidium giganteum metabolites against mosquitoes after flash chromatography.
abstract: Entomopathogens are significant natural enemies for mosquitoes. We have investigated the adulticidal efficacies of metabolites of Trichophyton ajelloi and Lagenidium giganteum against Culex quinquefasciatus and Aedes aegypti simultaneously. The T. ajelloi was grown on Sabouraud's dextrose broth medium at 25 ± 2°C and relative humidity at 75 ± 5% for 15 days. L. giganteum was grown in peptone yeast extract glucose broth at 25 ± 2°C and relative humidity 75 ± 5% for 15 days. The filtrations of metabolites have been made by using Whatman-1 filter paper then with the flash chromatograph. The bioassays were conducted as per the World Health Organization's methods and protocols (2006). In this significant investigations, the metabolites of T. ajelloi have been found highly susceptible against A. aegypti with LC(99)-7.24 ml after an exposure time of 24 h with a comparison, the LC(99)-66 ml was observed against C. quinquefasciatus after exposure of 24 h. Moreover, the L. giganteum metabolites have shown higher pathogenicity against C. quinquefasciatus with LC(99)-11.3 ml and A. aegypti with LC(99)-15.49 ml. Although, the efficacy in adults could be achieved with higher concentration can be significant also. Their adulticidal activities in different climatic zones are plausible with metabolites which have better LT values of T. ajelloi.
authors.affiliation: Department of Zoology, Environmental and Advanced Parasitology and Vector Control Biotechnology Laboratories, Dayalbagh Educational Institute, Dayalbagh, Agra, 282 005, India. singhgavendra@gmail.com
authors.forename: Gavendra
authors.initials: G
authors.lastname: Singh
authors.forename: Soam
authors.initials: S
authors.lastname: Prakash
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001681:Biological Assay; D002845:Chromatography / Q000379:methods; D003465:Culex / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D005374:Filtration / Q000379:methods; D006801:Humans; D007306:Insecticides / Q000302:isolation & purification* / Q000494:pharmacology*; D044726:Lagenidium / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D009172:Mycology / Q000379:methods; D016019:Survival Analysis; D014249:Trichophyton / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D003470:Culture Media; D007306:Insecticides