title: Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages.
abstract: We have found a novel type of pluripotent stem cells, Multilineage-differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.
authors.affiliation: Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Wakao
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitada
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Mari
authors.initials: M
authors.lastname: Dezawa
mesh_terms: D000328:Adult; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D058625:End Stage Liver Disease / Q000628:therapy*; D005434:Flow Cytometry / Q000379:methods; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3