title: Protein recognition by a self-assembled deep cavitand monolayer on a gold substrate.
abstract: This paper details the first use of a self-folding deep cavitand on a gold surface. A sulfide-footed deep, self-folding cavitand has been synthesized, and its attachment to a cleaned gold surface studied by electrochemical and SPR methods. Complete monolayer formation is possible if the cavitand folding is templated by noncovalent binding of choline or by addition of space-filling thiols to cover any gaps in the cavitand adsorption layer. The cavitand is capable of binding trimethylammonium-tagged guests from an aqueous medium and can be deposited in 2 × 2 microarrays on the surface for characterization by SPR imaging techniques. When biotin-labeled guests are used, the cavitand:guest construct can recognize and immobilize streptavidin proteins from aqueous solution, acting as an effective supramolecular biosensor for monitoring protein recognition.
authors.affiliation: Department of Chemistry, University of California, Riverside, Riverside, California 92521, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Taira
authors.forename: Michael C
authors.initials: MC
authors.lastname: Young
authors.forename: Dariush
authors.initials: D
authors.lastname: Ajami
authors.forename: Julius
authors.initials: J
authors.lastname: Rebek
authors.forename: Quan
authors.initials: Q
authors.lastname: Cheng
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Hooley
mesh_terms: D006046:Gold / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011506:Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D011506:Proteins; D006046:Gold