title: [Mouse-induced pluripotent stem cells have the potential to differentiate into induced primordial germ cells].
abstract: OBJECTIVE
  To investigate whether mouse-induced pluripotent stem (iPS) cell line IP14D-1 has the potential to differentiate into induced primordial germ cells (iPGCs), and to explore the changes in the expression of iPGCs-differentiation associated genes and their possible mechanisms.
  
  
  METHODS
  Undifferentiated IP14D-1 was cultured to proliferate and then differentiated to form 4-, 7- and 9-day-old induced embryoid bodies (iEBs) in vitro, respectively. RT-PCR and immunofluorescence were used to detect the expressions of Lin28, Blimpl, Stra8 and Mvh, as well as the localization of the corresponding protein in iEBs.
  
  
  RESULTS
  The expression of Blimpl was higher than that of Lin28 in the undifferentiated IP14D-1 and mouse embryonic stem cells (mESCs). Mvh and Stra8 as well as mESCs and EBs were also expressed in IP14D-1 and iEBs, but with no significant differences. The expression of Lin28 was gradually increased in the IP14D-1-derived iEBs from 4 to 7 days, but decreased at 9 days, and the expression of Blimp1 was gradually reduced with the prolonged growing time of iEBs.
  
  
  CONCLUSIONS
  A stable system was established for the culture and differentiation of IP14D-1 and IP14D-1-derived iEBs. The expressions of Lin28, Blimp1, Mvh and Stra8 were not significantly different between the undifferentiated IP14D-1 and mESCs, nor were the expressions of Mvh and Stra8 between iEBs and EBs. IP14D-1 and iEBs had the potential to differentiate into iPGCs, which increased in number in the 7-day-old iEBs, and the expression of iPGC-differentiation associated Lin28 became lower in the older iEBs.
authors.affiliation: Key Laboratory of Male Reproduction and Genetics of Guangdong Province, Shenzhen Hospital of Peking University, Shenzhen, Guangdong 518036, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Yan-min
authors.initials: YM
authors.lastname: Zhang
authors.forename: Zheng-yu
authors.initials: ZY
authors.lastname: Qi
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Guang-hui
authors.initials: GH
authors.lastname: Cui
authors.forename: Yao-ting
authors.initials: YT
authors.lastname: Gui
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Cai
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology; D005854:Germ Cells / Q000166:cytology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C