title: Alteration in viability and proliferation activity of mitogen stimulated Jurkat cells.
abstract: The aim of our study was the establishment of mechanisms of alterations in viability and mitogen stimulated T lymphocyte proliferation activity. In PHA-stimulated Jurkat cells along with increased proliferation level the reduction of mitochondrial dehydrogenases activity (by 20%) and rising number of cells with low mitochondrial membrane potential (ΔΨm) was revealed.   It was concluded that interaction of mitogen (PHA) with T cells receptors (TCR) contributes reduction activity of mitochondrial NADH-dehydrogenase (via phospolipasa C-diacilglicerol (PLC-DAG)-induced PKCs (PKCα, PKCθ) activation pathway). Supression activity of I complex (NADH-dehydrogenase) of mitochondrial respiratory chain and reduction of mitochondrial membrane potencial (ΔΨm) is accompanied with intensification of superoxide radicals production. Superoxide radicals as secondary messengers involve in the induction of T cells viability and proliferative activity regulating genes (CD95L and IL-2) and by this way contribute modification of cell proliferation and apoptosis intensity.
authors.affiliation: Tbilisi State Medical University, Tbilisi, Georgia.
authors.forename: G
authors.initials: G
authors.lastname: Lomsadze
authors.forename: N
authors.initials: N
authors.lastname: Gogebashvili
authors.forename: M
authors.initials: M
authors.lastname: Enukidze
authors.forename: M
authors.initials: M
authors.lastname: Machavariani
authors.forename: N
authors.initials: N
authors.lastname: Intskirveli
authors.forename: T
authors.initials: T
authors.lastname: Sanikidze
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D019169:Jurkat Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000201:enzymology; D010835:Phytohemagglutinins / Q000494:pharmacology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D010835:Phytohemagglutinins; D011948:Receptors, Antigen, T-Cell; D013481:Superoxides