title: microRNA-1301-mediated inhibition of tumorigenesis.
abstract: The relatively recent discovery of microRNAs has added a completely new             dimension to the study of the regulation of tumor cells, but how they control             cell behavior remains largely elusive. HepG2 cells were assigned to the miR-1301             group and the control group. RT-PCR, Western blotting, wound healing, the Transwell             chamber migration and MTT assays, and apoptosis detection assays were used to             analyze cell behavior of HepG2 cells after miR-1301 mimic transfection. Our study             showed that miR-1301 was downregulated in HepG2 cells, and that miR-1301 inhibited             migration and invasion of HepG2 cells and promoted cellular apoptosis after transfection             with miR-1301 mimics. In addition, p53 mRNA and p53 protein expression was upregulated,             and Bcl-2 and Bcl-xL mRNA and protein expression was downregulated in the miR-1301             group. These results indicate that miR-1301 may be an inhibitor of tumorigenesis             in HepG2 cells.
authors.affiliation: Fifth Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Fang
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.forename: Guang-Shun
authors.initials: GS
authors.lastname: Yang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C494811:BCL2L1 protein, human; C572423:MIRN1301 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051020:bcl-X Protein