title: 2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves.
abstract: Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is the result of biochemical and physiological processes, such as enhancement of the antioxidant enzymatic system and accumulation of UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke is an attractive species for studying the protein network involved in UV stress response, being characterized by remarkable levels of inducible antioxidants. Proteomic tools can assist the evaluation of the expression patterns of UV-responsive proteins and we applied the difference in-gel electrophoresis (DIGE) technology for monitoring the globe artichoke proteome variation at four time points following an acute UV-C exposure. A total of 145 UV-C-modulated proteins were observed and 119 were identified by LC-MS/MS using a ∼144,000 customized Compositae protein database, which included about 19,000 globe artichoke unigenes. Proteins were Gene Ontology (GO) categorized, visualized on their pathways and their behaviour was discussed. A predicted protein interaction network was produced and highly connected hub-like proteins were highlighted. Most of the proteins differentially modulated were chloroplast located, involved in photosynthesis, sugar metabolisms, protein folding and abiotic stress. The identification of UV-C-responsive proteins may contribute to shed light on the molecular mechanisms underlying plant responses to UV stress.
authors.affiliation: DIVAPRA, University of Turin, Grugliasco, Torino, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Falvo
authors.forename: Mariasole
authors.initials: M
authors.lastname: Di Carli
authors.forename: Angiola
authors.initials: A
authors.lastname: Desiderio
authors.forename: Eugenio
authors.initials: E
authors.lastname: Benvenuto
authors.forename: Andrea
authors.initials: A
authors.lastname: Moglia
authors.forename: Twan
authors.initials: T
authors.lastname: America
authors.forename: Sergio
authors.initials: S
authors.lastname: Lanteri
authors.forename: Alberto
authors.initials: A
authors.lastname: Acquadro
mesh_terms: D031943:Cynara scolymus / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D010940:Plant Proteins / Q000032:analysis* / Q000145:classification*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D010940:Plant Proteins