title: Biochemical characterization of a testis-predominant isoform of N-alpha acetyltransferase.
abstract: N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is a common protein -modification process in eukaryotic cells. Despite its widespread occurrence, the biological significance of this modification process is still unclear. We recently discovered a novel testis-predominant isoform of the catalytic subunit of the enzyme complex. Here, we describe the biochemical characterization of this testis-predominant N-alpha acetyltransferase complex, which includes protein-protein interaction study by co-immunoprecipitation experiment and functional study by N-alpha acetyltransferase assay.
authors.affiliation: Section on Clinical Genomics, Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. panga@mail.nih.gov
authors.forename: Alan Lap-Yin
authors.initials: AL
authors.lastname: Pang
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D016466:CHO Cells; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D057075:Enzyme Assays / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000201:enzymology* / Q000378:metabolism; D014162:Transfection
chemical_list: D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D000123:Acetyltransferases