title: Sesaminol glucosides protect β-amyloid induced apoptotic cell death by regulating redox system in SK-N-SH cells.
abstract: We have investigated the neuroprotective effect of sesaminol glucosides (SG) in SK-N-SH cells. SG prevented apoptotic cell death induced by Aβ₂₅₋₃₅. In parallel, SK-N-SH cells exposed to Aβ₂₅₋₃₅ underwent oxidative stress as shown by the elevated level of intracellular ROS, lipid peroxidation, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation, which were effectively suppressed by SG treatment. Furthermore, SG reversed the activities of catalase and glutathione peroxidase, and restored intracellular GSH levels in Aβ₂₅₋₃₅ challenged SK-N-SH cells. In addition, SG inhibited not only Aβ₂₅₋₃₅-induced apoptotic features including cleavage of poly(ADP-ribose) polymerase, activation of caspase-3, and activation of caspase-9, but also elevated Bax/Bcl-2 ratio in SK-N-SH cells treated with Aβ₂₅₋₃₅. It was also observed that Aβ₂₅₋₃₅ stimulated the phosphorylation of mitogen-activated protein kinases (MAPKs), including extracellular protein regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAP kinase. SG inhibited phosphorylation of the JNK, ERK and p38 MAP kinase. These results suggest that SG has a protective effect against Aβ₂₅₋₃₅-induced neuronal apoptosis, possibly through scavenging oxidative stress and regulating MAPKs signaling pathways.
authors.affiliation: Division of Metabolism and Functionality Research, Korea Food Research Institute, 516 Baekhyun-Dong, Bundang-Gu, Sungnam, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Um
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Ahn
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Tae Youl
authors.initials: TY
authors.lastname: Ha
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D004149:Dioxoles / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000633:toxicity*; D012715:Sesame Oil / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D004149:Dioxoles; D005663:Furans; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C066016:sesaminol; D012715:Sesame Oil