title: Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor.
abstract: Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor.
authors.affiliation: Guangdong Provincial Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Chu
authors.forename: Huhui
authors.initials: H
authors.lastname: Chen
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Ding
authors.forename: Liwen
authors.initials: L
authors.lastname: Jiang
authors.forename: Edward W T
authors.initials: EW
authors.lastname: Tsang
authors.forename: Keqiang
authors.initials: K
authors.lastname: Wu
authors.forename: Shangzhi
authors.initials: S
authors.lastname: Huang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D017302:Annexins / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics / Q000502:physiology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D031653:Nelumbo / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D017302:Annexins; D018076:DNA, Complementary; D010940:Plant Proteins; D011994:Recombinant Proteins