title: Simultaneous determination of diloxanide furoate and metronidazole in presence of diloxanide furoate degradation products.
abstract: Three methods are presented for the simultaneous determination of diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least squares were applied. These two approaches were successfully applied to quantify each drug in the mixture using the information included in, the absorption spectra in the range of 225-320 nm. The second method is TLC-densitometry, in which the binary mixture and degradates were separated on silica gel plates using a chloroform-acetone-glacial acetic acid (9.5 + 0.5 + 0.07, v/v/v) mobile phase and the bands were scanned at 254 nm. The last method is HPLC, in which DLX, MTR, and degradates were separated using the mobile phase acetonitrile-0.05 M dibasic potassium phosphate (25 + 75, v/v), adjusted to pH 4 with orthophosphoric acid, at a flow rate of 1 mL/min, on a C18 analytical column. Detection was at 254 nm. The proposed methods were successfully applied for the analysis of DLX and MTR in pharmaceutical formulations, and the results were statistically compared with a reported spectrophotometric method.
authors.affiliation: Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr El-Aini St., 11562 Cairo, Egypt.
authors.forename: Samah S
authors.initials: SS
authors.lastname: Abbas
authors.forename: Nour E
authors.initials: NE
authors.lastname: Wagieh
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdelkawy
authors.forename: Maha M
authors.initials: MM
authors.lastname: Abdelrahman
mesh_terms: D000563:Amebicides / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003720:Densitometry; D004338:Drug Combinations; D004355:Drug Stability; D004356:Drug Storage; D005663:Furans / Q000032:analysis*; D007202:Indicators and Reagents; D008795:Metronidazole / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000032:analysis
chemical_list: D000563:Amebicides; D004338:Drug Combinations; D005663:Furans; D007202:Indicators and Reagents; D013607:Tablets; D008795:Metronidazole; C004932:diloxanide furoate