title: Fermentation of reactive-membrane-extracted and ammonium-hydroxide-conditioned dilute-acid-pretreated corn stover.
abstract: Acid-pretreated biomass contains various compounds (acetic acid, etc.) that are inhibitory to fermentative microorganisms. Removing or deactivating these compounds using detoxification methods such as overliming or ammonium hydroxide conditioning (AHC) improves sugar-to-ethanol yields. In this study, we treated the liquor fraction of dilute-acid-pretreated corn stover using AHC and a new reactive membrane extraction technique, both separately and in combination, and then the sugars in the treated liquors were fermented to ethanol with the glucose-xylose-fermenting bacterium, Zymomonas mobilis 8b. We performed reactive extraction with mixtures of octanol/Alamine 336 or oleyl alcohol/Alamine 336. The best ethanol yields and rates were achieved for oleyl alcohol-extracted hydrolysates followed by AHC hydrolysates, while octanol-extracted hydrolysates were unfermentable because highly toxic octanol was found in the hydrolysate. Adding olive oil significantly improved yields for octanol-extracted hydrolysate. Additional work is underway to determine if this technology is a cost-effective alternative to traditional hydrolysate conditioning processes.
authors.affiliation: Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523-2370, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Grzenia
authors.forename: S Ranil
authors.initials: SR
authors.lastname: Wickramasinghe
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Schell
mesh_terms: D000143:Acids / Q000737:chemistry / Q000302:isolation & purification; D064753:Ammonium Hydroxide; D005233:Fatty Alcohols / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D006878:Hydroxides / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008567:Membranes, Artificial; D000442:Octanols / Q000737:chemistry; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*; D017355:Zymomonas / Q000378:metabolism
chemical_list: D000143:Acids; D005233:Fatty Alcohols; D006878:Hydroxides; D008567:Membranes, Artificial; D000442:Octanols; D000069463:Olive Oil; D010938:Plant Oils; C010268:oleyl alcohol; D064753:Ammonium Hydroxide