title: The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence.
abstract: Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn(2+) or under oxidative stress in vitro, and significantly decreased virulence.
authors.affiliation: USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
authors.forename: Fany
authors.initials: F
authors.lastname: Reffuveille
authors.affiliation: AgroParisTech, UMR Micalis, F-78350 Jouy-en-Josas, France.|INRA, UMR1319 Micalis, F-78350 Jouy-en-Josas, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Serror
authors.affiliation: Laboratoire de Microbiologie du Froid, Signaux et Micro-Environnement, UPRES EA4312, Université de Rouen, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
authors.affiliation: USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Budin-Verneuil
authors.affiliation: USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
authors.forename: Rabia
authors.initials: R
authors.lastname: Ladjouzi
authors.affiliation: Plateforme Proteogen IFR ICORE 146, Université de Caen, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Bernay
authors.affiliation: USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
authors.forename: Yanick
authors.initials: Y
authors.lastname: Auffray
authors.affiliation: USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Rincé
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000401:mortality; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera; D008345:Manganese / Q000378:metabolism; D016019:Survival Analysis; D014166:Transferases / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D037521:Virulence Factors; D008345:Manganese; D014166:Transferases; C042308:phosphatidylglycerol glyceryl transferase