title: Characterization of a new dog isolate of canine distemper virus from China.
abstract: Canine distemper virus (CDV) is a highly contagious pathogen of dogs. Vaccination is an effective way to protect dogs from CDV infection, but occasionally fails. In the present study, a wild type (wt) CDV, named XJ2, was isolated from a dead vaccinated dog. The hemagglutinin (H) gene of the XJ2 was amplified and analyzed for the molecular characteristics including N-glycosylation sites, phylogenesis, hydrophobicity and epitopes. The data indicated that XJ2 was a genetic variant strain of CDV. CDV-sero-negative dogs were inoculated intranasally with XJ2, developed severe clinical symptoms and died, suggesting high virulence.
authors.affiliation: Department of Veterinary Medicine, Shihezi University, Shihezi, Xinjiang, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Qiao
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Xia
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: Y
authors.initials: Y
authors.lastname: Ren
authors.forename: H
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D002522:Chlorocebus aethiops; D004216:Distemper / Q000401:mortality / Q000517:prevention & control / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004285:Dogs; D014644:Genetic Variation; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014611:Vaccination / Q000662:veterinary; D014709:Vero Cells; D014774:Virulence
chemical_list: D006389:Hemagglutinins, Viral