title: Effects of electric potential treatment of a chromium hexacyanoferrate modified biosensor based on PQQ-dependent glucose dehydrogenase.
abstract: A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The potential treatment of CrHCF, which converts Fe(II) to Fe(III), enables the glucose detection. The amperometric measurement linearity of the biosensor was up to 20 mM (R = 0.9923), and the detection sensitivity was 199.94 nA/mM per cm(2). More importantly, this biosensor remained stable for >270 days.
authors.affiliation: Biomedical Engineering Department, Chung-Yuan Christian University, No. 200, Zhongbei Rd., Zhongli City, Taoyuan County 32023, Taiwan. g9402502@cycu.edu.tw
authors.forename: Ta-Feng
authors.initials: TF
authors.lastname: Tseng
authors.forename: Yang-Li
authors.initials: YL
authors.lastname: Yang
authors.forename: Yuh-Jiuan
authors.initials: YJ
authors.lastname: Lin
authors.forename: Shyh-Liang
authors.initials: SL
authors.lastname: Lou
mesh_terms: D015374:Biosensing Techniques; D017608:Chromium Compounds / Q000737:chemistry*; D004560:Electricity; D005290:Ferric Compounds / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D017608:Chromium Compounds; D005290:Ferric Compounds; D005295:Ferrocyanides; D005296:Ferrous Compounds; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); C020354:hexacyanoferrate II; D005947:Glucose