title: Synapsin peptide fused to E. coli heat-labile toxin B subunit induces regulatory T cells and modulates cytokine balance in experimental autoimmune encephalomyelitis.
abstract: We previously found that the preventive oral administration of a hybrid consisting of the C domain of synapsin and the B subunit of E. coli heat-labile enterotoxin (LTBSC) efficiently suppresses experimental autoimmune encephalomyelitis (EAE) development in rats. We investigated the effect of LTBSC on cytokine expression and on regulatory T (Treg) cells in rats with myelin induced EAE. LTBSC treatment increased the frequency of CD4(+)FoxP3(+) Treg cells in lymph nodes prior to challenge and in the EAE acute stage. LTBSC also up-regulated the expression of anti-inflammatory Th2/Th3 cytokines and diminished myelin basic protein-specific Th1 and Th17 cell responses in lymph nodes. CD4(+)CD25(+) Treg cells from LTBSC treated rats showed stronger suppressive properties than Treg cells from controls in vitro. Our observations indicate that LTBSC is a useful agent for modulating the autoimmune responses in EAE.
authors.affiliation: Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC, UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, X5000HUA Córdoba, Argentina.
authors.forename: M Julia
authors.initials: MJ
authors.lastname: Scerbo
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Bibolini
authors.forename: German A
authors.initials: GA
authors.lastname: Roth
authors.forename: Clara G
authors.initials: CG
authors.lastname: Monferran
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage*; D002417:Cattle; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000276:immunology; D004768:Enterotoxins / Q000008:administration & dosage*; D029968:Escherichia coli Proteins / Q000008:administration & dosage*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D008297:Male; D004676:Myelin Basic Protein / Q000037:antagonists & inhibitors; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016704:Synapsins / Q000235:genetics / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D001427:Bacterial Toxins; D016207:Cytokines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D018836:Inflammation Mediators; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D016704:Synapsins; C027538:heat-labile enterotoxin, E coli