title: Novel cell-penetrating peptides based on α-aminoxy acids.
abstract: The remarkable ability of cell-penetrating peptides (CPPs) to deliver cell-impermeable compounds into living cells makes them attractive transporters for use in biology and medicine. Despite their highly efficient cellular uptake, CPPs consisting of natural amino acids always suffer from degradation and endosomal entrapment, thereby greatly limiting their application in vivo. Here, we describe the preparation of novel CPPs incorporating α-aminoxy acid residues and their cellular uptake behavior. We demonstrate that introducing α-aminoxy acids into the backbones of CPPs enhances their diffuse cytosolic distribution after direct membrane translocation. We also reveal a hybrid peptide, consisting of D-α-aminoxy acids and L-α-amino acids, that achieves efficient diffuse distribution in the cytosol, is stable toward serum, and possesses low cytotoxicity, thus making it a possible vector candidate for in vivo applications. Our results confirm that α-aminoxy acids are useful building blocks when designing novel CPPs possessing favorable properties.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-, Huazhong University of Science and Technology (HUST), Wuhan, China.
authors.forename: Yilong
authors.initials: Y
authors.lastname: Ma
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D014018:Tissue Distribution
chemical_list: D000596:Amino Acids; D057846:Cell-Penetrating Peptides