title: Analysis of the role of RhoGDI1 and isoprenylation in the degradation of RhoGTPases.
abstract: RhoGDI1 is one of the three major regulators of the Rho switch along with RhoGEFs and RhoGAPs. RhoGDI1 extracts prenylated Rho proteins from lipid membranes, sequesters them in the cytosol, and prevents nucleotide exchange or hydrolysis. In addition, RhoGDI1 protects prenylated Rho proteins from degradation. Here, we describe techniques to monitor Rho proteins degradation upon depletion of RhoGDI1 and their dependence upon prenylation for degradation.
authors.affiliation: Institut National de la Santé et de la Recherche Mé dicale Avenir Team, U634, Sophia-Antipolis University, Nice, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Boulter
authors.forename: Rafael
authors.initials: R
authors.lastname: Garcia-Mata
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008148:Lovastatin / Q000494:pharmacology; D017368:Protein Prenylation / Q000187:drug effects / Q000502:physiology*; D034741:RNA, Small Interfering; D014162:Transfection; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
chemical_list: D020730:Guanine Nucleotide Dissociation Inhibitors; D034741:RNA, Small Interfering; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D008148:Lovastatin; D020741:rho GTP-Binding Proteins