title: Yeasts as a tool for heterologous gene expression.
abstract: The yeasts Saccharomyces cerevisiae and Pichia pastoris are attractive hosts for production of human proteins. The main advantages offered by these systems are the well-developed and easily accessible genetic tools, rapid growth, the simple and inexpensive culture media, and many of the cellular and metabolic processes found in higher eukaryotes are conserved in both yeast species. In this chapter, we describe the production of two proteins of therapeutic interest: the human P53 tumor suppressor and the viral HBsAg in P. pastoris and S. cerevisiae using the strong and inducible promoters AOX1 and Gal10/Cyc1, respectively. Besides the production as a goal of both expressions, we also report on an unexpected result that has occurred in S. cerevisiae: The overexpression of human p53 induces yeast cell death with characteristic markers of apoptosis, such as the externalization of phosphatidylserines and DNA strand cleavage.
authors.affiliation: Centre de Biotechnologie de Sfax, Sfax, Tunisia. raja.gargouri@cbs.rnrt.tn
authors.forename: Raja
authors.initials: R
authors.lastname: Mokdad-Gargouri
authors.forename: Salma
authors.initials: S
authors.lastname: Abdelmoula-Soussi
authors.forename: Nadia
authors.initials: N
authors.lastname: Hadiji-Abbès
authors.forename: Ines Yacoubi-Hadj
authors.initials: IY
authors.lastname: Amor
authors.forename: Istabrak
authors.initials: I
authors.lastname: Borchani-Chabchoub
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
mesh_terms: D042931:Aldehyde Oxidase / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006801:Humans; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: D003470:Culture Media; C518355:GAL10 protein, S cerevisiae; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53; C526436:AOX1 protein, human; D042931:Aldehyde Oxidase