title: [Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10].
abstract: OBJECTIVE
  To investigate the expression of RhoA in the lung tissue of acute lung injury (ALI) rats induced by oleic acid (OA) and explore the influence of RhoA on the level of IL-8 and  IL-10 in serum. Discussion RhoA and acute lung injury relationship.
  
  
  METHODS
  Establish rat oleic acid acute lung injury model,  Wet/Dry weights (W/D) were detected. Pathological changes of the lung tissue were examined. The levels of IL-8 and IL-10 in serum were detected by ELISA and the expression of RhoA in homogenate of lung tissue was determined by PCR.
  
  
  RESULTS
  W/D and ALI score in ALI group at 6,  12, 24 and 48 h were higher than those in control group (P<0.01) and intervention group at the same time point (P<0.05). The level of IL-10(ng/L) in serum in ALI group at  2,  6,  12, 24 and 48 h was remarkably higher than that in control group (P<0.01),  but was lower compared with intervention group at the same time point (P<0.01). The level of IL-8(pg/L) in serum at  2, 6, 12, 24 and 48 h in ALI group were higher than those in control group(P<0.01)  and intervention group at the same time point(P<0.01).Compared with that of control group,  the expression of RhoA in homogenate of lung tissue in ALI group at 2,  6, 12, 24 and 48 h was higher(P<0.01),  but there was no difference between ALI group and intervention group at the same time point(P>0.05).
  
  
  CONCLUSIONS
  The expression of RhoA in lung tissue of ALI rats induced by OA is increased. RhoA expression increase that can cause lung damage degree increase in rats. RhoA can aggravate the pathology changes of ALI rats,  which might be related to up regulating expression of IL-8 and down regulating expression of IL-10 by RhoA.
authors.affiliation: Department of Pediatrics Binzhou Medical University, Binzhou 256603, China. cuiqie1028@163.com
authors.forename: Qing-qing
authors.initials: QQ
authors.lastname: Cui
authors.forename: Xue-bin
authors.initials: XB
authors.lastname: Feng
authors.forename: Yue-si
authors.initials: YS
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D016753:Interleukin-10 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008168:Lung / Q000378:metabolism*; D008297:Male; D019301:Oleic Acid; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000502:physiology*
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger; D016753:Interleukin-10; D019301:Oleic Acid; D020742:rhoA GTP-Binding Protein