title: Bioconjugate of lysozyme and the antibacterial marine sesquiterpene quinone avarone and its derivatives.
abstract: A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
authors.affiliation: IHTM, Center for Chemistry, University of Belgrade , Njegoševa 12, Belgrade, Serbia. irenan@chem.bg.ac.rs
authors.forename: Irena
authors.initials: I
authors.lastname: Novaković
authors.forename: Uroš
authors.initials: U
authors.lastname: Anđelković
authors.forename: Mario
authors.initials: M
authors.lastname: Zlatović
authors.forename: Miroslav J
authors.initials: MJ
authors.lastname: Gašić
authors.forename: Dušan
authors.initials: D
authors.lastname: Sladić
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D053138:Cyclohexenes / Q000737:chemistry* / Q000494:pharmacology*; D004789:Enzyme Activation; D008826:Microbial Sensitivity Tests; D056004:Molecular Dynamics Simulation; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000494:pharmacology*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D053138:Cyclohexenes; D012717:Sesquiterpenes; C046374:avarone; D009113:Muramidase