title: Bacterial isolation by lectin-modified microengines.
abstract: New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionalized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for the rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine the selective capture of E. coli with the uptake of polymeric drug-carrier particles to provide an attractive motion-based theranostics strategy. Triggered release of the captured bacteria is demonstrated by movement through a low-pH glycine-based dissociation solution. The smaller size of the new polymer-metal microengines offers convenient, direct, and label-free optical visualization of the captured bacteria and discrimination against nontarget cells.
authors.affiliation: Department of Nanoengineering, University of California-San Diego, La Jolla, California 92093, USA.
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.forename: Jahir
authors.initials: J
authors.lastname: Orozco
authors.forename: Daniel
authors.initials: D
authors.lastname: Kagan
authors.forename: Maria
authors.initials: M
authors.lastname: Guix
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Sirilak
authors.initials: S
authors.lastname: Sattayasamitsathit
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Claussen
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000302:isolation & purification*; D037102:Lectins / Q000737:chemistry* / Q000648:ultrastructure; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D037102:Lectins; D020409:Molecular Motor Proteins