title: Functionalization of multi-walled carbon nanotubes with coumarin derivatives and their biological evaluation.
abstract: We report the synthesis and the characterization of different multi-walled carbon nanotubes (MWCNTs) linked to natural molecules, 5,7-coumarins and/or oleic acid, obtained from purified pristine MWCNTs by a cascade of chemical functionalization. The activities of these modified MWCNTs were investigated in vitro on human umbilical vein endothelial cells (HUVECs) by evaluating their ability to influence cell viability and to induce cell apoptosis. Our data showed that pristine MWCNTs are markedly cytotoxic; conversely, the carboxylated carbon nanotubes, much more readily dispersed in aqueous solutions and CNT-Link, the key intermediate designed by us for the drug anchorage, are biocompatible at the tested concentrations (1 and 10 μg ml(-1)).
authors.affiliation: Dipartimento Farmaco-Chimico, Università di Messina, Via S.S. Annunziata, 98168, Messina, Italy. diannazzo@unime.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Iannazzo
authors.forename: Anna
authors.initials: A
authors.lastname: Piperno
authors.forename: Angelo
authors.initials: A
authors.lastname: Ferlazzo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pistone
authors.forename: Candida
authors.initials: C
authors.lastname: Milone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Lanza
authors.forename: Francesco
authors.initials: F
authors.lastname: Cimino
authors.forename: Antonio
authors.initials: A
authors.lastname: Speciale
authors.forename: Domenico
authors.initials: D
authors.lastname: Trombetta
authors.forename: Antonina
authors.initials: A
authors.lastname: Saija
authors.forename: Signorino
authors.initials: S
authors.lastname: Galvagno
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D019301:Oleic Acid / Q000737:chemistry
chemical_list: D003374:Coumarins; D037742:Nanotubes, Carbon; D019301:Oleic Acid