title: LPS-induced TNFα factor (LITAF) in the snail Cipangopaludina chinensis: gene cloning and its apoptotic effect on NCI-H446 cells.
abstract: LPS-induced TNFα factor (LITAF) is a transcription factor mediating TNF-α expression under LPS stimulation, and playing important roles in immune responses. In the present study, partial cDNA sequence of a LITAF (designated CcLITAF) gene was cloned and identified from snail Cipangopaludina chinensis. It contains an open reading frame of 348 nucleotides encoding a predicted protein of 115 amino acids, with a conserved LITAF domain at C-terminal, and shares a similarity ranging from 34% to 96% with other LITAF from oyster to mammals. CcLITAF mRNA ubiquitously expressed in all analyzed tissues. Interestingly, cLITAF could induce apoptosis in human tumor cell line, NCI-H446 cells, and caspase 3 play key roles in CcLITAF-mediated apoptosis. Present studies provide new insight into the biological function of CcLITAF.
authors.affiliation: College of Life Sciences, Shaoxing University, No. 900, South City Road, Shaoxing 312000, China. shiouby@hotmail.com
authors.forename: Shoubao
authors.initials: S
authors.lastname: Yang
authors.forename: Peifen
authors.initials: P
authors.lastname: Li
authors.forename: Zhongxiang
authors.initials: Z
authors.lastname: Mi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D006801:Humans; D008070:Lipopolysaccharides; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000494:pharmacology / Q000633:toxicity; D016415:Sequence Alignment; D012908:Snails / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha