title: Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon.
abstract: OBJECTIVE
  To evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats.
  
  
  METHODS
  Eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. The animals were divided into three experimental groups and one control group: EI = pneumoperitoneum for 30 minutes before laparotomy (n=20); EII = pneumoperitoneum for 30 minutes after abdominal closure (n=20); EIII = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); C = control group, without pneumoperitoneum (n=20). In each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. A segment of the abdominal wall was resected and subjected to tensile strength testing. Another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin.
  
  
  RESULTS
  There were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery.
  
  
  CONCLUSIONS
  Under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.
authors.affiliation: Laboratory of Experimental Surgery,Medical School, University of Brasilia (UnB), Brazil.
authors.forename: Pedro Henrique Alves de
authors.initials: PH
authors.lastname: Morais
authors.forename: Igor Eduardo Caetano de
authors.initials: IE
authors.lastname: Farias
authors.forename: Leonardo de Castro
authors.initials: Lde C
authors.lastname: Durães
authors.forename: Fabiana Pirani
authors.initials: FP
authors.lastname: Carneiro
authors.forename: Paulo Gonçalves de
authors.initials: PG
authors.lastname: Oliveira
authors.forename: João Batista de
authors.initials: JB
authors.lastname: Sousa
mesh_terms: D034861:Abdominal Wall / Q000601:surgery*; D000714:Anastomosis, Surgical; D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage*; D003106:Colon / Q000601:surgery*; D004353:Drug Evaluation, Preclinical; D008297:Male; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects* / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013718:Tensile Strength / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D002245:Carbon Dioxide