title: Co-delivery of antigen and a lipophilic anti-inflammatory drug to cells via a tailorable nanocarrier emulsion.
abstract: Nanotechnology promises new drug carriers that can be tailored to specific applications. Here we report a new approach to drug delivery based on tailorable nanocarrier emulsions (TNEs), motivated by a need to co-deliver a protein antigen and a lipophilic drug for specific inhibition of nuclear factor kappa B (NF-κB) in antigen presenting cells (APCs). Co-delivery for NF-κB inhibition holds promise as a strategy for the treatment of rheumatoid arthritis. We used a highly surface-active peptide (SAP) to prepare a nanosized emulsion having defined surface properties predictable from the SAP sequence. Incorporating the lipophilic drug into the oil phase at the time of emulsion formation enabled its facile packaging. The SAP is depleted from bulk during emulsification, allowing simple subsequent addition of the drug-loaded oil-in-water emulsion to a solution of protein antigen. Decoration of emulsion surface with antigen was achieved via electrostatic deposition. In vitro data showed that the TNE prepared this way was internalized and well-tolerated by model APCs, and that good suppression of NF-κB expression was achieved. This work reports a new type of nanotechnology-based carrier, a TNE, which can potentially be tailored for co-delivery of multiple therapeutic components, and can be made using simple methods using only biocompatible materials.
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St. Lucia, QLD 4072, Australia.
authors.forename: Yap Pang
authors.initials: YP
authors.lastname: Chuan
authors.forename: Bi Yun
authors.initials: BY
authors.lastname: Zeng
authors.forename: Brendan
authors.initials: B
authors.lastname: O'Sullivan
authors.forename: Ranjeny
authors.initials: R
authors.lastname: Thomas
authors.forename: Anton P J
authors.initials: AP
authors.lastname: Middelberg
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000938:Antigen-Presenting Cells / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053758:Nanoparticles; D036103:Nanotechnology; D009821:Oils; D010316:Particle Size; D010446:Peptide Fragments / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D004655:Emulsions; D016328:NF-kappa B; D009821:Oils; D010446:Peptide Fragments; D013501:Surface-Active Agents; D014867:Water; D003474:Curcumin