title: A novel mono-carbonyl analogue of curcumin induces apoptosis in ovarian             carcinoma cells via endoplasmic reticulum stress and reactive oxygen species production.
abstract: The aim of the present study was to investigate the apoptosis of human ovarian             cancer cell lines, A2780 and CP70, induced by a novel curcumin analogue, B19.             The proliferation of cells was detected with methyl thiazolyl tetrazolium (MTT)             assay and apoptosis was examined by flow cytometry. Reactive oxygen species (ROS)             were assessed by the fluorescent indicator DCF-DA. The protein expression of the             endoplasmic reticulum (ER) stress pathways, GRP78, XBP-1, ATF-4 and CHOP, was             examined with Western blotting. A growth inhibitory effect was observed after             treatment with B19 in a dose-dependent manner and with more potential than curcumin.             At 20 µM, B19 induced significant apoptosis in CP70 cells. Furthermore, B19 induced             the ER stress response, while curcumin had no effect on ER stress. These results             suggest that B19 has more effective antitumor properties than curcumin, and is             associated with the activation of ER stress and ROS in ovarian cancer cells.
authors.affiliation: Key Laboratory of Biotechnology Pharmaceutical Engineering, School of Pharmaceutical Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, PR China.
authors.forename: Xie
authors.initials: X
authors.lastname: Zhang
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zhu
authors.forename: Yu-Huang
authors.initials: YH
authors.lastname: Wang
authors.forename: Xi-Chong
authors.initials: XC
authors.lastname: Yu
authors.forename: Xin-Bo
authors.initials: XB
authors.lastname: Zhu
authors.forename: Guang
authors.initials: G
authors.lastname: Liang
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Li
mesh_terms: D051701:Activating Transcription Factor 4 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma / Q000378:metabolism; D045744:Cell Line, Tumor; D003474:Curcumin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D006360:Heat-Shock Proteins; D006801:Humans; D007659:Ketones / Q000737:chemistry; D010051:Ovarian Neoplasms / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D000071701:Regulatory Factor X Transcription Factors; D051742:Transcription Factor CHOP / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D000071717:X-Box Binding Protein 1
chemical_list: C571782:(1E,4E)-1,5-bis(2-methoxyphenyl)penta-1,4-dien-3-one; C497109:ATF4 protein, human; C497249:DDIT3 protein, human; D004268:DNA-Binding Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D007659:Ketones; D017382:Reactive Oxygen Species; D043562:Receptors, G-Protein-Coupled; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; D051701:Activating Transcription Factor 4; D051742:Transcription Factor CHOP; D003474:Curcumin