title: Polymorphic variations in manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPX1), and catalase (CAT) contribute to elevated plasma triglyceride levels in Chinese patients with type 2 diabetes or diabetic cardiovascular disease.
abstract: Manganese superoxide dismutase (MnSOD), glutathione peroxidase-1 (GPX1), and catalase (CAT) provide the primary antioxidant defense system. Impaired antioxidant defense increases oxidative stress and contributes to the development of type 2 diabetes and diabetic cardiovascular disease (CVD). We preformed a case-control study in Chinese type 2 diabetes patients, to determine if the MnSOD Val16Ala (T→C), GPX1 Pro198Leu (C→T), and CAT -262C/T (C→T) functional polymorphisms contribute to the development of type 2 diabetes or diabetic CVD. Patients with type 2 diabetes (n = 168) were divided into the non-CVD group (n = 83, >10 year since diagnosis) and CVD group (n = 85, history of ischemic CVD). Genotyping was performed using PCR-restriction fragment length polymorphism (PCR-RFLP) or PCR-based direct sequencing. The genotypic distribution in the non-CVD- and CVD-group and the clinical parameters in genotypic groups were not significantly different in the three polymorphic sites, respectively. Among eight genotypic combinations, the most common TT+CC+CC genotype (59.5%) was associated with higher triglyceride levels than the TT+CT+CC genotype, the second frequent one (14.9%; 1.77 ± 0.12 vs. 1.21 ± 0.11 mmol/l, P = 0.001), and all non-TT+CC+CC genotypes (40.5%; 1.77 ± 0.12 vs. 1.43 ± 0.12 mmol/l, P = 0.048). In the CVD group, significantly elevated triglyceride levels were also observed in patients with TT+CC+CC compared to patients with TT+CT+CC (2.00 ± 0.18 vs. 1.37 ± 0.16 mmol/l, P = 0.018) or non-TT+CC+CC genotypes (2.00 ± 0.18 vs. 1.65 ± 0.19 mmol/l, P = 0.070). The common MnSOD, GPX1, and CAT TT+CC+CC genotype may contribute to hypertriglyceridemia in Chinese patients with type 2 diabetes or diabetic CVD.
authors.affiliation: Department of Endocrinology & Metabolism, Shanghai Diabetes Institute, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Ruie
authors.initials: R
authors.lastname: Zhao
authors.forename: Qihan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Wenrui
authors.initials: W
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Lu
authors.forename: Taishan
authors.initials: T
authors.lastname: Zheng
authors.forename: Jiamei
authors.initials: J
authors.lastname: Jiang
authors.forename: Weijing
authors.initials: W
authors.lastname: Zhao
authors.forename: Kunsan
authors.initials: K
authors.lastname: Xiang
authors.forename: Weiping
authors.initials: W
authors.lastname: Jia
authors.forename: Limei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000097:blood / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D048909:Diabetes Complications / Q000097:blood / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018570:Risk Assessment; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000235:genetics*; D014280:Triglycerides / Q000097:blood*; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D014280:Triglycerides; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C000722067:GPX1 protein, human