title: Polar body morphology is not predictive of its cell division origin.
abstract: OBJECTIVE
  This study sought to determine whether morphology of simultaneously biopsied polar bodies is predictive of their cell division origin.
  
  
  METHODS
  Levels of heterozygosity were measured using single nucleotide polymorphism (SNP) microarrays in sequentially biopsied polar bodies to establish the predictive value on samples with known cell division origins. The validated method of predicting cell division origin of polar bodies using heterozygosity rates was then applied to simultaneously biopsied polar bodies which had origin predictions made by morphological assessment.
  
  
  RESULTS
  SNP microarray heterozygosity analysis was proven to be 94% predictive when tested against the known origin of sequentially biopsied polar bodies (n = 133). This methodology subsequently demonstrated that morphology was only 63% consistent when tested on simultaneously biopsied polar bodies (n = 455; P < 0.0001). Predictions of the origins of aneuploidy using morphology assignment were also significantly different than with heterozygosity assignment of polar body cell division origin (P = 0.0003).
  
  
  CONCLUSIONS
  Studies of the origin of aneuploidy utilizing morphological assignment of polar body cell division origin without heterozygosity analysis may be inaccurate and should be interpreted with caution.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA. ntreff@rmanj.com
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Jessyca
authors.initials: J
authors.lastname: Campos
authors.forename: John
authors.initials: J
authors.lastname: Stevens
authors.forename: William
authors.initials: W
authors.lastname: Schoolcraft
authors.forename: Mandy
authors.initials: M
authors.lastname: Katz-Jaffe
mesh_terms: D000782:Aneuploidy; D002455:Cell Division / Q000235:genetics; D005260:Female; D006579:Heterozygote; D006801:Humans; D008540:Meiosis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009865:Oocytes / Q000502:physiology / Q000648:ultrastructure*; D059705:Polar Bodies / Q000648:ultrastructure*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011237:Predictive Value of Tests; D011247:Pregnancy