title: Interaction of 5-fluorouracil loaded nanoparticles with 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomes used as a cellular membrane model.
abstract: Nuclear magnetic resonance (NMR) spectroscopy and steady-state fluorescence anisotropy were used to study the behavior and interaction of 5-fluorouracil, both in a free form (5FU) and included in the polymer matrix of poly(butylcyanoacrylate) nanoparticles (5FUPBCN) with a phospholipid bilayer of large unilammellar vesicles composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), as a model system of biomembranes. The results confirm an interaction and penetration of 5FU into the phospholipid bilayer of DMPC liposomes. Different mechanisms of drug transfer from the aqueous environment into the model membrane environment, for the free drug and that incorporated into polymer nanoparticles, are suggested: (i) concentration-dependent reversible diffusion of the free 5FU and (ii) sustained 5FU release from nanoparticles adsorbed on the liposome surface resulting in continuous delivery of the drug into the phospholipid bilayers of the DMPC liposomes.
authors.affiliation: REQUIMTE, Departamento de Química, Faculdade de Ciências, Universidade do Porto, Porto, Portugal.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lopes
authors.forename: Margarita
authors.initials: M
authors.lastname: Simeonova
authors.forename: Paula
authors.initials: P
authors.lastname: Gameiro
authors.forename: Maria
authors.initials: M
authors.lastname: Rangel
authors.forename: Galya
authors.initials: G
authors.lastname: Ivanova
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005454:Fluorescence Polarization; D005472:Fluorouracil / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D005472:Fluorouracil; D004134:Dimyristoylphosphatidylcholine