title: B-SOLANA: an approach for the analysis of two-base encoding bisulfite sequencing data.
abstract: CONCLUSIONS
  Bisulfite sequencing, a combination of bisulfite treatment and high-throughput sequencing, has proved to be a valuable method for measuring DNA methylation at single base resolution. Here, we present B-SOLANA, an approach for the analysis of two-base encoding (colorspace) bisulfite sequencing data on the SOLiD platform of Life Technologies. It includes the alignment of bisulfite sequences and the determination of methylation levels in CpG as well as non-CpG sequence contexts. B-SOLANA enables a fast and accurate analysis of large raw sequence datasets.
  
  
  METHODS
  The source code, released under the GNU GPLv3 licence, is freely available at http://code.google.com/p/bsolana/.
  
  
  BACKGROUND
  b.kreck@ikmb.uni-kiel.de
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Institute of Clinical Molecular Biology, Christian-Albrechts-University, Kiel, Germany. b.kreck@ikmb.uni-kiel.de
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kreck
authors.forename: George
authors.initials: G
authors.lastname: Marnellos
authors.forename: Julia
authors.initials: J
authors.lastname: Richter
authors.forename: Felix
authors.initials: F
authors.lastname: Krueger
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
authors.forename: Andre
authors.initials: A
authors.lastname: Franke
mesh_terms: D019175:DNA Methylation; D015894:Genome, Human; D055106:Genome-Wide Association Study; D006801:Humans; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013447:Sulfites
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite