title: Neuroprotective effects of osthole pretreatment against traumatic brain injury in rats.
abstract: Osthole, a coumarin compound isolated from the plant-derived herb Cnidium monnieri, has been the subject of considerable interest because of its broad spectrum of pharmacological properties. The aim of this study was to investigate the potential protective effects of osthole in adult rats in the setting of traumatic brain injury (TBI). We employed Feeney's weight-drop model to ascertain whether intraperitoneal administration of osthole (10mg/kg, 20mg/kg and 40 mg/kg) 30 min before TBI could reduce the severity of neurological deficits, cerebral edema, and hippocampal neuron loss. The levels of malondialdehyde (MDA) and glutathione (GSH), the activity of superoxide dismutase (SOD), the expressions of Bcl-2, Bax, and active caspase-3, and the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive apoptotic cells were also measured to characterize the antioxidative and antiapoptotic properties. A significant reduction of neurological deficits, cerebral edema and hippocampal neuron loss was observed in the osthole pretreatment groups (20mg/kg and 40 mg/kg, but not 10mg/kg) by 24h after TBI compared with the TBI group. Furthermore, pretreatment with osthole (40 mg/kg) significantly increased the activity of SOD, the level of GSH, and the ratio of Bcl-2/Bax, and also reduced the level of MDA, the expression of active caspase-3, and the number of apoptotic cells at 24h after TBI. In summary, these results suggested that osthole had a neuroprotective effect against TBI, and the protection may be associated with its antioxidative and antiapoptotic functions.
authors.affiliation: Department of Neurosurgery, Xijing Institute of Clinical Neuroscience, Xijing Hospital, Fourth Military Medical University, No. 127, Changle Western Road, Xi'an, Shanxi 710032, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: He
authors.forename: Shuoyao
authors.initials: S
authors.lastname: Qu
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Haining
authors.initials: H
authors.lastname: Zhen
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control*; D031048:Cnidium / Q000737:chemistry; D003374:Coumarins / Q000008:administration & dosage*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003374:Coumarins; D018696:Neuroprotective Agents; C046627:osthol