title: Characterization of the 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) degradation system in Janibacter sp. TYM3221.
abstract: Bacterial degradation of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) has been previously reported, however, its degradation enzyme system has not been characterized. In this study, a DDE-degrading bacterium, Janibacter sp. TYM3221, was isolated and characterized. Transformation of DDE was demonstrated by TYM3211 resting cells grown in LB in the presence and absence of biphenyl. Gas chromatography-mass spectrometry analysis revealed five metabolites of DDE containing a meta-ring cleavage product and 4-chlorobenzoic acid, suggesting that TYM3221 degrades DDE to 4-chlorobenzoic acid via a meta-ring cleavage product. A gene cluster, bphAaAbAcAd, which codes for biphenyl dioxygenase subunits, was cloned from TYM3221. A mutant strain with a bphAa-gene inactivation did not grow on biphenyl, and showed no DDE degradation activity. These results indicate that in strain TYM3221, the bphAa-coded biphenyl dioxygenase is involved not only in the metabolism of biphenyl but also in the degradation of DDE.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Anh Thi Phuong
authors.initials: AT
authors.lastname: Nguyen
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Takumi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tokuda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kasai
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D003633:Dichlorodiphenyl Dichloroethylene / Q000378:metabolism*; D005798:Genes, Bacterial; D007306:Insecticides / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D003633:Dichlorodiphenyl Dichloroethylene; D007306:Insecticides; D010105:Oxygenases; C010574:biphenyl