title: Differentiation of prostate cancer cells using flexible fluorescent polymers.
abstract: Using water-soluble, fluorescent, flexible polymers, we have devised a novel methodology for identification and differentiation of prostate cancer cells. Using a stepwise linear discriminant analysis, we demonstrate that the differential modulations of the polymer emission intensities in the presence of conditioned cell culture media can be used to distinguish between prostate cancer subtypes and between cancerous and noncancer cells. The differences in the compositions of the conditioned cell culture media are likely contributing to different fluorescence spectral patterns of the polymers. This in vitro approach may provide a novel platform for the development of an alternative prostate cancer diagnostic and subtyping technique.
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, North Dakota 58108-6050, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Scott
authors.forename: Rinku
authors.initials: R
authors.lastname: Dutta
authors.forename: Manas K
authors.initials: MK
authors.lastname: Haldar
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Friesner
authors.forename: Sanku
authors.initials: S
authors.lastname: Mallik
mesh_terms: D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008297:Male; D011108:Polymers / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000473:pathology*
chemical_list: D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D011108:Polymers