title: Rapid in vivo conversion of effector T cells into Th2 cells during helminth infection.
abstract: Stimulation of the immune system by pathogens, allergens, or autoantigens leads to differentiation of CD4(+) T cells with pro- or anti-inflammatory effector cell functions. Based on functional properties and expression of characteristic cytokines and transcription factors, effector CD4(+) T cells have been grouped mainly into Th1, Th2, Th17, and regulatory T (Treg) cells. At least some of these T cell subsets remain responsive to external cues and acquire properties of other subsets, raising the hope that this functional plasticity might be exploited for therapeutic purposes. In this study, we used an Ag-specific adoptive transfer model and determined whether in vitro-polarized or ex vivo-isolated Th1, Th17, or Treg cells can be converted into IL-4-expressing Th2 cells in vivo by infection of mice with the gastrointestinal helminth Nippostrongylus brasiliensis. Th1 and Th17 cells could be repolarized to acquire the expression of IL-4 and lose the expression of their characteristic cytokines IFN-γ and IL-17A, respectively. In contrast, both in vitro-generated and ex vivo-isolated Treg cells were largely resistant to repolarization. The helminth-induced conversion of Th1 or Th17 cells into Th2 cells may partially explain the inverse correlation between helminth infection and protection against autoimmune disorders.
authors.affiliation: Institute for Immunology, Ludwig Maximilian University, 80336 Munich, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Panzer
authors.forename: Selina
authors.initials: S
authors.lastname: Sitte
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wirth
authors.forename: Ingo
authors.initials: I
authors.lastname: Drexler
authors.forename: Tim
authors.initials: T
authors.lastname: Sparwasser
authors.forename: David
authors.initials: D
authors.lastname: Voehringer
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D016764:Cell Polarity / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009559:Nippostrongylus / Q000276:immunology; D017206:Strongylida Infections / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000469:parasitology / Q000637:transplantation; D018417:Th1 Cells / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D058504:Th17 Cells / Q000276:immunology* / Q000473:pathology / Q000637:transplantation; D018418:Th2 Cells / Q000276:immunology* / Q000469:parasitology / Q000473:pathology
chemical_list: D018984:Epitopes, T-Lymphocyte; D015847:Interleukin-4; D007371:Interferon-gamma