title: DNA-binding and photocleavage studies of ruthenium(II) complexes containing asymmetric intercalative ligand.
abstract: A novel asymmetric ligand 2-(pyridine-2-yl)-1-H-imidazo[4,5-i]dibenzo[2,3-a:2',3'-c]phenazine (pidbp) and its ruthenium complexes [Ru(L)(2)(pidbp)](2+) (L=bpy (2, 2'- bipyridine), phen (1, 10 - phenanthroline)), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR. Various methods support the conclusion that both Ru(II) complexes can intercalate into DNA base pairs. Complex [Ru(bpy)(2)(pidbp)](2+)4 exhibits its DNA "molecular light switch" properties. Furthermore, the two complexes are efficient DNA-photocleavers under irradiation at 365 nm, and complex 5 exhibits a stronger DNA-photocleavage efficiency than complex 4. The mechanism of DNA cleavage is an oxidative process by generating singlet oxygen.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, ChangDe 415000, PR China. liuxuewen050@sina.com
authors.forename: Xue-Wen
authors.initials: XW
authors.lastname: Liu
authors.forename: Yuan-Dao
authors.initials: YD
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Lu
authors.forename: Da-Shun
authors.initials: DS
authors.lastname: Zhang
mesh_terms: D000042:Absorption / Q000528:radiation effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000528:radiation effects*; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism*; D008024:Ligands; D008027:Light; D010957:Plasmids / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017186:Titrimetry; D014783:Viscosity / Q000528:radiation effects
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D012428:Ruthenium; D004247:DNA; D004996:Ethidium