title: Thyroid-related autoantibodies in Tunisian patients with type 1 diabetes.
abstract: OBJECTIVE
  To evaluate, retrospectively, the frequency of antithyroid antibodies (ATA) in patients with type 1 diabetes (T1D).
  
  
  METHODS
  Antithyroperoxidase antibodies (TPO-Ab), antithyroglobulin antibodies (TG-Ab), and antithyroid-stimulating hormone receptor antibodies (TSHR-Ab) were determined by enzyme-linked immunosorbent assay. Sera of 312 patients (166 children and 146 adults) with T1D were analyzed. Sera of 276 healthy subjects (87 children and 189 blood donors) served as controls.
  
  
  RESULTS
  Out of 312 patients with T1D, 44 (14%) had ATA (TPO-Ab or TG-Ab or TSHR-Ab). The frequency of ATA in patients with T1D was significantly higher than in the control group (14% vs. 2.8%; p<10(-5)). ATA were significantly more frequent in adult patients with T1D than in the blood donor group (20% vs. 1.6%; p<10(-8)). The frequency of ATA in adult patients was significantly higher than in pediatric patients (20% vs. 9%; p=0.006). The frequency of TPO-Ab and TG-Ab was significantly higher in patients with T1D than in the control group (13.5% vs. 2%; p<10(-8) and 7% vs. 2.2%, p=0.008), respectively. Out of 312 patients with T1D, only one had TSHR-Ab. The simultaneous presence of three autoantibodies was found in one patient with T1D.
  
  
  CONCLUSIONS
  ATA were frequent in patients with T1D. Serological screening of autoimmune thyroid disease is suggested in patients with T1D.
authors.affiliation: Research Unit (03/UR/07-02), Faculty of Pharmacy, Monastir, Tunisia.
authors.forename: Wahiba
authors.initials: W
authors.lastname: Sakly
authors.forename: Amani
authors.initials: A
authors.lastname: Mankaï
authors.forename: Achouak
authors.initials: A
authors.lastname: Achour
authors.forename: Yosra
authors.initials: Y
authors.lastname: Thabet
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ouertani
authors.forename: Lamia
authors.initials: L
authors.lastname: Boughammoura
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Harbi
authors.forename: Laarbi
authors.initials: L
authors.lastname: Chaieb
authors.forename: Mohamed Taher
authors.initials: MT
authors.lastname: Sfar
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ghedira
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007223:Infant; D007453:Iodide Peroxidase / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011989:Receptors, Thyrotropin / Q000276:immunology; D012189:Retrospective Studies; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology*; D014416:Tunisia
chemical_list: D001323:Autoantibodies; D011989:Receptors, Thyrotropin; C006606:anti-thyroglobulin; D013954:Thyroglobulin; D007453:Iodide Peroxidase