title: Isolation and purification method of mouse fetal hepatoblasts.
abstract: During development, liver precursors constitute a valuable source of pluripotent stem cells that present the ability to differentiate into both a hepatic and biliary lineage. In the present chapter, we report an experimental procedure developed by our group to isolate mouse fetal hepatoblasts (MFHs) with high purity. The method is based on a selective harvesting of the hepatic parenchymal cells from fetuses (E 14.5), followed by the sorting of E-cadherin(+) progenitors through the use of magnetic beads and specific antibodies. This protocol allows the isolation of bipotent liver stem cells expressing both hepatic and biliary markers. Primary cultures of purified MFHs can be maintained under proliferation until confluence, leading to promotion of the differentiation process in the presence of hepatotrophic factors. By using a quantitative real-time polymerase chain reaction approach, we show the hepatospecific phenotype and the progressive maturation of MFHs, delineating early (α-fetoprotein), mid (albumin), and late (glucose-6-phosphatase) hepatic markers. Consequently, the model appears to be a valuable cell system for the study of molecular and cellular aspects occurring in hepatic differentiation.
authors.affiliation: Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan. lgailhou@ncc.go.jp
authors.forename: Luc
authors.initials: L
authors.lastname: Gailhouste
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D005333:Fetus / Q000166:cytology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D000418:Albumins; D015415:Biomarkers; D000509:alpha-Fetoproteins; D005952:Glucose-6-Phosphatase