title: [Wnt/b-catenin signaling pathway affects the protein expressions of caspase-3, XIAP and Grp-78 in hepatocellular carcinoma].
abstract: To investigate the relationship and significance of Wnt/b-catenin signaling pathway with caspase-3, XIAP, HSP27and Grp-78. The HCC cell line HepG2 was transfected with small interfering RNA (siRNA) directed against b-catenin. After 72 and 96 h, protein was extracted and the protein expressions of b-catenin, caspase-3, XIAP, Grp-78 and HSP27 were detected by Western blot. b-catenin protein expression was inhibited at both time points and the expression at 96 h was higher than that at 72 h (F = 160.72, P is less than to 0.01). Interestingly, Caspase-3 protein expression was decreased at 72 h and increased to normal at 96 h (F = 136.10, P is less than to 0.01), while p-caspase-3 protein expression increased at 72 h and decreased to normal at 96 h (F = 98.65, P is less than to 0.01). XIAP protein expression decreased at 72 h (F = 37.29, P is less than to 0.01) and increased at 96 h. Grp-78 protein expression increased at 72 h and decreased to normal at 96 h ( F = 58.72, P is less than to 0.01). HSP27 protein expression showed no change following transfection ( F = 1.91, P is more than to 0.05). Wnt/b-catenin signaling pathway is related to the protein expressions of caspase-3, XIAP and Grp-78, but not related to HSP27 protein expression in HCC. Wnt/b-catenin signaling pathway may participate in the regulation of HCC apoptosis, proliferation and differentiation through affecting these factors.
authors.affiliation: Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130012, China.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Meng
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.forename: Ya-Ju
authors.initials: YJ
authors.lastname: DU
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Fan
mesh_terms: D006528:Carcinoma, Hepatocellular; D053148:Caspase 3; D051177:Catenins; D006801:Humans; D008113:Liver Neoplasms; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051177:Catenins; D051176:beta Catenin; D053148:Caspase 3