title: Double-application of platelet-rich plasma on bone healing in rabbits.
abstract: OBJECTIVE
  Platelet-rich plasma (PRP) is considered to enhance bone formation especially at early stages of wound healing, depending on the limited and short life-span of platelets and growth factors. The aim of this study was to evaluate efficacy of double-application of PRP (DA-PRP) on bone healing in a rabbit calvarial defect model.
  
  
  METHODS
  Twenty-eight rabbits, each had two surgically prepared calvarial bone defects (10mm diameter), were included in this study and randomly divided into six groups. Defects (n=56) were treated with single-application of PRP (SA-PRP)(n=10), SA-PRP and beta-tricalciumphosphate (SA-PRP+TCP)(n=10), DA-PRP (n=8), DA-PRP and beta-tricalciumphosphate (DA-PRP+TCP)(n=8), beta-tricalciumphosphate (TCP)(n=10) or left empty (Control)(n=10). Animals were sacrificed at 30 days postoperatively.
  
  
  RESULTS
  The new bone (NB%) and defect fill (DF%) percentages were calculated from histological slides by image-analyzer software and statistically analysed. All test groups showed higher NB% than control, but differences among all groups were insignificant. The TCP treated groups had significantly higher DF% than groups treated without TCP, however the DF% differences between control, SA-PRP and DA-PRP or TCP, SA-PRP+TCP or DA-PRP+TCP were insignificant.
  
  
  CONCLUSIONS
  Although new bone formation was histomorphologically remarkable at double-application PRP groups, statistical analyses of the histomorphometric data revealed no significant difference.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. burcucakilci@yahoo.com
authors.forename: Burcu
authors.initials: B
authors.lastname: Özdemir
authors.forename: Bülent
authors.initials: B
authors.lastname: Kurtiş
authors.forename: Gülay
authors.initials: G
authors.lastname: Tüter
authors.forename: Burcu
authors.initials: B
authors.lastname: Sengüven
authors.forename: Benay
authors.initials: B
authors.lastname: Tokman
authors.forename: Selin
authors.initials: S
authors.lastname: Pınar-Özdemir
authors.forename: İlkim
authors.initials: İ
authors.lastname: Demirel
authors.forename: Gönen
authors.initials: G
authors.lastname: Özcan
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology; D005260:Female; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D011817:Rabbits; D014945:Wound Healing