title: Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog.
abstract: Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 μl microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark. jens.f.rehfeld@rh.regionh.dk
authors.forename: Jens F
authors.initials: JF
authors.lastname: Rehfeld
authors.forename: Peter
authors.initials: P
authors.lastname: Ericsson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood*; D002766:Cholecystokinin / Q000276:immunology*; D005755:Gastrins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D057230:Limit of Detection; D017551:Microdialysis; D008969:Molecular Sequence Data; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D005755:Gastrins; D002766:Cholecystokinin