title: Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.
abstract: We investigated whether mesenchymal stem cells (MSCs) in synovial fluid (SF) increased in the knee with degenerated cartilage and osteoarthritis. SF was obtained from the knee joints of 22 patients with anterior cruciate ligament (ACL) injury during ACL reconstruction, and cartilage degeneration was evaluated arthroscopically. SF was also obtained from the knee joints of 6 healthy volunteers, 20 patients with mild osteoarthritis, and 26 patients with severe osteoarthritis, in which the grading was evaluated radiographically. The cell component in the SF was cultured for analyses. Synovium (SYN) and bone marrow (BM) were also harvested during total knee arthroplasties. The MSC number in SF was correlated with the cartilage degeneration score evaluated by arthroscopy. The MSC number in the SF was hardly noticed in normal volunteers, but it increased in accordance with the grading of osteoarthritis. Though no significant differences were observed regarding surface epitopes, or differentiation potentials, the morphology and gene profiles in SF MSCs were more similar to those in SYN MSCs than in BM MSCs. We listed 20 genes which were expressed higher in both SYN MSCs and SF MSCs than in BM MSCs, and 3 genes were confirmed by quantitative RT-PCR. MSCs in SF increased along with degenerated cartilage and osteoarthritis.
authors.affiliation: Section of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. sekiya.orj@tmd.ac.jp
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekiya
authors.forename: Miyoko
authors.initials: M
authors.lastname: Ojima
authors.forename: Shiro
authors.initials: S
authors.lastname: Suzuki
authors.forename: Mika
authors.initials: M
authors.lastname: Yamaga
authors.forename: Masafumi
authors.initials: M
authors.lastname: Horie
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Koga
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tsuji
authors.forename: Ken
authors.initials: K
authors.lastname: Miyaguchi
authors.forename: Soichi
authors.initials: S
authors.lastname: Ogishima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Muneta
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000293:Adolescent; D000328:Adult; D016118:Anterior Cruciate Ligament / Q000473:pathology*; D000070598:Anterior Cruciate Ligament Injuries; D001854:Bone Marrow Cells / Q000166:cytology; D002357:Cartilage Diseases / Q000473:pathology*; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology*; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D020219:Chondrogenesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007719:Knee Joint; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000473:pathology*; D010012:Osteogenesis / Q000235:genetics; D013582:Synovial Fluid / Q000166:cytology*; D055815:Young Adult