title: Electrospun fibrous scaffold of hydroxyapatite/poly (ε-caprolactone) for bone regeneration.
abstract: Development of fibrous scaffold of hydroxyapatite/biopolymer nanocomposite offers great potential in the field of bone regeneration and tissue engineering. Hydroxyapatite (HA)/poly (ε-caprolactone) (PCL) fibrous scaffolds were successfully prepared by electrospinning dopes containing HA and PCL in this work. It was found that pre-treating HA with γ-glycioxypropyltrimethoxysilane (A-187) was effective in improving HA dispersion both in solutions and in a PCL matrix. Mechanical properties of the scaffolds were greatly enhanced by the filling of A187-HA. The bioactivity of PCL was remarkably improved by the addition of HA and A187-HA. Fibroblasts and osteoblasts were seeded on scaffolds to evaluate the effect of A-187 on biocompatibility of HA/PCL composites. Based on this study, good dispersion of HA in PCL matrix was granted by pretreatment of HA with A-187 and A187-HA/PCL fibrous scaffolds were obtained by electrospinning. These results demonstrated that the scaffolds may possess improved mechanical performance and good bioactivity due to A187-HA incorporation.
authors.affiliation: Affiliated Eye Hospital, Wenzhou Medical College, Wenzhou, Zhejiang, People's Republic of China. lll09182@126.com
authors.forename: Lingli
authors.initials: L
authors.lastname: Li
authors.forename: Guang
authors.initials: G
authors.lastname: Li
authors.forename: Jianming
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaona
authors.initials: X
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.forename: Kaihui
authors.initials: K
authors.lastname: Nan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D001842:Bone and Bones; D002460:Cell Line; D004563:Electrochemistry / Q000379:methods; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters / Q000737:chemistry*; D012995:Solubility; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer