title: A novel plant cell bioproduction platform for high-yield secretion of recombinant proteins.
abstract: Plant cell suspension culture integrates the merits of whole-plant systems with those of microbial fermentation and mammalian cell culture, and has been recognized as a promising alternative biosynthetic platform for valuable proteins. However, the low protein productivity dilemma has been the bottleneck toward commercializing this technology. Here, we describe a new technology, termed hydroxyproline (Hyp)-Glyco technology, that dramatically increases the yield of secreted recombinant proteins from cultured plant cells by expressing them as fusions with a novel glycomodule tag comprising an Hyp-rich repetitive peptide (HypRP) backbone that is subsequently glycosylated through the Hyp residues. The extensive glycosylation of the HypRP tags greatly extends the serum half-life of small therapeutic proteins, such as interferon α2b or human growth hormone, without significantly impairing their bioactivities and the tag greatly enhances solubility.
authors.affiliation: Arkansas Biosciences Institute, Arkansas State University, State University, AR, USA. jxu@astate.edu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: Kieliszewski
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D015153:Blotting, Western; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000302:isolation & purification; D006031:Glycosylation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism*; D016898:Interferon-alpha / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010455:Peptides / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D012995:Solubility; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D006023:Glycoproteins; D016898:Interferon-alpha; D009841:Oligonucleotides; D010455:Peptides; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D006909:Hydroxyproline