title: External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells.
abstract: Small-cell lung cancer (SCLC) is a highly malignant carcinoma with poor long-term survival. Effective treatment remains highly demanded. In the present study, we demonstrated that External Qi of Yan Xin Qigong (YXQ-EQ) exerted potent cytotoxic effect towards SCLC cell line NCI-H82 via induction of apoptosis. Global gene expression profiling identified 39 genes whose expression was altered by YXQ-EQ in NCI-82 cells. Among them, semi-quantitative RT-PCR and real-time qPCR analyses confirmed that the gene expression levels of apoptotic proteins death-associated protein kinase 2 and cell death-inducing DFFA-like effector b were upregulated, whereas that of oncoproteins DEK and MYCL1, cell migration-promoting proteins CD24 and integrin-alpha 9, and glycolytic enzyme aldolase A were downregulated. These findings suggest that YXQ-EQ may exert anticancer effect through modulating gene expression in a way that facilitates cancer cell apoptosis while represses proliferation, metastasis, and glucose metabolism.
authors.affiliation: The Institute of Chongqing Traditional Chinese Medicine, Chongqing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Igor
authors.initials: I
authors.lastname: Dozmorov
authors.forename: Mark Barton
authors.initials: MB
authors.lastname: Frank
authors.forename: Ming
authors.initials: M
authors.lastname: Dao
authors.forename: Michael
authors.initials: M
authors.lastname: Centola
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Dan
authors.initials: D
authors.lastname: Hu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D064550:Death-Associated Protein Kinases; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D015513:Oncogene Proteins / Q000235:genetics; D000075223:Poly-ADP-Ribose Binding Proteins; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D019413:Qi; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051603:Recoverin / Q000235:genetics; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; C112511:CIDEB protein, human; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D004365:Drugs, Chinese Herbal; C489427:MYC protein, human; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D016271:Proto-Oncogene Proteins c-myc; C496731:RCVRN protein, human; D012333:RNA, Messenger; D051603:Recoverin; C579104:DAPK2 protein, human; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose