title: New erythrocyte and reticulocyte parameters on CELL-DYN Sapphire: analytical and preanalytical aspects.
abstract: BACKGROUND
  Extended RBC and reticulocyte parameters are useful in diagnosing functional iron deficiency and various other clinical conditions. The newest software of the CELL-DYN Sapphire measures extended RBC and reticulocyte parameters. The aims of the present communication were to assess the analytical aspects of these parameters compared with the Siemens Advia 120 analyzer, to study the effect of sample aging and to briefly explore their clinical usefulness in patients with anemia.
  
  
  METHODS
  Blood samples were obtained from the routine workload of two hospital laboratories and were run on Siemens Advia and Abbott CELL-DYN Sapphire analyzers in parallel. Data analysis was performed using standard statistics.
  
  
  RESULTS
  In total, 1416 patient samples were analyzed. There was close correlation in microcytic and macrocytic RBC (r(2) > 0.97) with small bias. The hypo- and hyperchromic RBC showed reasonable correlations, Advia 120 giving higher values. Reticulocyte MCV showed acceptable agreement, with significant proportional bias (Advia 8-9% higher). CELL-DYN Sapphire MCHr correlated rather well with Advia CHr (r(2) > 0.91) with significant absolute bias. Remarkably, the correlation data differed significantly between the two laboratories. It was found that aging of EDTA samples had significant effect on most of the RBC parameters.
  
  
  CONCLUSIONS
  The new RBC parameters of CELL-DYN Sapphire generally correlated well with those of Advia 120, although significant systematic differences were present, particularly in reticulocyte MCH and MCV. These differences necessitate instrument-specific reference ranges and clinical decision values. To minimize preanalytical effects, these parameters should be measured in fresh blood samples.
authors.affiliation: Clinical Laboratory, Amphia Hospital, Breda, the Netherlands.
authors.forename: A A M
authors.initials: AA
authors.lastname: Ermens
authors.forename: J J M L
authors.initials: JJ
authors.lastname: Hoffmann
authors.forename: M
authors.initials: M
authors.lastname: Krockenberger
authors.forename: E M
authors.initials: EM
authors.lastname: Van Wijk
mesh_terms: D000367:Age Factors; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D004920:Erythropoiesis; D005260:Female; D006403:Hematologic Tests; D006405:Hematology / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008297:Male; D011247:Pregnancy; D015203:Reproducibility of Results; D017701:Reticulocyte Count / Q000379:methods*; D012156:Reticulocytes / Q000737:chemistry* / Q000166:cytology