title: [Monitoring the prevalence of pathogenic Borrelia burgdorferi sensu lato in ticks in two Brno locations].
abstract: OBJECTIVE
  The aim of the article is to present the results of monitoring the prevalence of the spirochete Borrelia burgdorferi sensu lato (Bbsl) in Ixodes ricinus ticks in two different locations in the city of Brno, Pisárky and Zamilovaný hájek (ZH). At the same time, factors potentially affecting the activity of ticks in these locations were studied. Results of the monitoring carried out in the first three spring months of the year 2011 are compared with 2010 results.
  
  
  METHODS
  In 2010-2011, Ixodes ricinus ticks were periodically sampled in two locations in Brno by flagging. Ticks were investigated by dark field microscopy (DFM) for the presence of spirochetes. DFM-positive samples from 2010 were tested by PCR to identify Bbsl.
  
  
  RESULTS
  In the first three spring months (March-May) of 2011, a total of 234 ticks were collected in Pisárky and 211 ticks in ZH. Positive samples detected by DFM accounted for 5.56 % in Pisárky and 25.12 % in ZH. PCR confirmed Bbsl in at least 75 %. Statistical analysis confirmed the dependence of tick activity on the air temperature in both locations.
  
  
  CONCLUSIONS
  Within the same city, seroprevalence was found to be different in two locations. High seroprevalence in ZH (25.12 %) could be attributed to focal spread of the disease from several sources, both ticks and reservoirs such as rodents. This may results in unfavourable epidemiological situation in Brno. On the other hand, the seroprevalence rates in Pisárky are consistent with findings from an earlier long-term study.
authors.affiliation: Department of animal physiology and immunology, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic. alenazak@sci.muni.cz
authors.forename: Alena
authors.initials: A
authors.lastname: Zákovská
authors.forename: Hana
authors.initials: H
authors.lastname: Kučerová
authors.forename: Helena
authors.initials: H
authors.lastname: Nejezchlebová
authors.forename: Naďa
authors.initials: N
authors.lastname: Bartoňková
authors.forename: Tereza
authors.initials: T
authors.lastname: Rašovská
authors.forename: Adam
authors.initials: A
authors.lastname: Norek
mesh_terms: D000818:Animals; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D018153:Czech Republic / Q000453:epidemiology; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012621:Seasons; D016036:Seroepidemiologic Studies