title: Aloin enhances cisplatin antineoplastic activity in B16-F10 melanoma cells by transglutaminase-induced differentiation.
abstract: Aloin, a natural anthracycline from aloe plant, is a hydroxyanthraquinone derivative shown to have antitumor properties. This study demonstrated that aloin exerted inhibition of cell proliferation, adhesion and invasion abilities of B16-F10 melanoma cells under non-cytotoxic concentrations. Furthermore, aloin induced melanoma cell differentiation through the enhancement of melanogenesis and transglutaminase activity. To improve the growth-inhibiting effect of anticancer agents, we found that the combined treatment of cells with aloin and low doses of cisplatin increases the antiproliferative activity of aloin. The results suggest that aloin possesses antineoplastic and antimetastatic properties, exerted likely through the induction of melanoma cell differentiation.
authors.affiliation: Department of Biology, University Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy. claudiotabolacci@tiscali.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Tabolacci
authors.forename: Stefania
authors.initials: S
authors.lastname: Rossi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lentini
authors.forename: Bruno
authors.initials: B
authors.lastname: Provenzano
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Turcano
authors.forename: Francesco
authors.initials: F
authors.lastname: Facchiano
authors.forename: Simone
authors.initials: S
authors.lastname: Beninati
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D004642:Emodin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008543:Melanins / Q000096:biosynthesis; D008546:Melanoma, Experimental; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D008543:Melanins; D013096:Spermine; D014442:Monophenol Monooxygenase; D011503:Transglutaminases; D004642:Emodin; D002945:Cisplatin; C006457:alloin