title: Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A.
abstract: We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n=6), which received the rhoptry vaccine (200(g/dose); G2 (adjuvant-challenged, n=4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n=3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6% and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.
authors.affiliation: Laboratório de Protozoologia, Departamento de Medicina Veterinária Preventiva, Universidade de Londrina - UEL, Postal Box 6001, 86050-970 Londrina, PR, Brazil.
authors.forename: Ivo Alexandre Leme
authors.initials: IA
authors.lastname: da Cunha
authors.forename: Dauton Luiz
authors.initials: DL
authors.lastname: Zulpo
authors.forename: Alexey Leon Gomel
authors.initials: AL
authors.lastname: Bogado
authors.forename: Luiz Daniel
authors.initials: LD
authors.lastname: de Barros
authors.forename: Alessandra
authors.initials: A
authors.lastname: Taroda
authors.forename: Michelle
authors.initials: M
authors.lastname: Igarashi
authors.forename: Italmar Teodorico
authors.initials: IT
authors.lastname: Navarro
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D001921:Brain / Q000469:parasitology; D049109:Cell Proliferation; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D062605:Quillaja Saponins; D012503:Saponins / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology / Q000517:prevention & control*; D014122:Toxoplasma / Q000276:immunology* / Q000378:metabolism; D014124:Toxoplasmosis, Animal / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D062605:Quillaja Saponins; D012503:Saponins; C046386:Quil A