title: Silica encapsulated SERS nanoprobe conjugated to the bacteriophage tailspike protein for targeted detection of Salmonella.
abstract: Silica-encapsulated Raman-reporter embedded SERS nanoprobes, named nanoaggregate embedded beads (NAEBs), were conjugated to the Salmonella specific tailspike protein (TSP) isolated from the P22 bacteriophage to enable a highly specific and ultrasensitive optical transduction platform. We demonstrate three successful surface conjugation strategies and highlight the detection of a single bacterium using SERS.
authors.affiliation: National Research Council Canada, Ottawa, Canada. lilin.tay@nrc-cnrc.gc.ca
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Tay
authors.forename: Ping-Ji
authors.initials: PJ
authors.lastname: Huang
authors.forename: Jamshid
authors.initials: J
authors.lastname: Tanha
authors.forename: Shannon
authors.initials: S
authors.lastname: Ryan
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wu
authors.forename: John
authors.initials: J
authors.lastname: Hulse
authors.forename: Lai-Kwan
authors.initials: LK
authors.lastname: Chau
mesh_terms: D017100:Bacteriophage P22 / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D006026:Glycoside Hydrolases; D006801:Humans; D049329:Nanostructures / Q000737:chemistry; D012475:Salmonella / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D017376:Viral Tail Proteins / Q000737:chemistry*
chemical_list: D017376:Viral Tail Proteins; D012822:Silicon Dioxide; D006026:Glycoside Hydrolases; C083268:tailspike protein, bacteriophage