title: Extraction of Lepidium apetalum seed oil using supercritical carbon dioxide and anti-oxidant activity of the extracted oil.
abstract: The supercritical fluid extraction (SFE) of Lepidium apetalum seed oil and its anti-oxidant activity were studied. The SFE process was optimized using response surface methodology (RSM) with a central composite design (CCD). Independent variables, namely operating pressure, temperature, time and flow rate were evaluated. The maximum extraction of Lepidium apetalum seed oil by SFE-CO₂ (about 36.3%) was obtained when SFE-CO₂ extraction was carried out under the optimal conditions of 30.0 MPa of pressure, 70 °C of temperature, 120 min of extraction time and 25.95 L/h of flow rate. GC-MS analysis showed the presence of four fatty acids in Lepidium apetalum seed oil, with a high content (91.0%) of unsaturated fatty acid. The anti-oxidant activity of the oil was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and 2,2'-azino- bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) test. Lepidium apetalum seed oil possessed a notable concentration-dependent antioxidant activity, with IC₅₀ values of 1.00 and 3.75 mg/mL, respectively.
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.
authors.forename: Kedan
authors.initials: K
authors.lastname: Chu
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.forename: Lidian
authors.initials: L
authors.lastname: Chen
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuchong
authors.initials: X
authors.lastname: Tang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D029686:Lepidium / Q000737:chemistry*; D008956:Models, Chemical; D010851:Picrates / Q000378:metabolism; D010938:Plant Oils / Q000302:isolation & purification* / Q000494:pharmacology; D012044:Regression Analysis; D012639:Seeds / Q000737:chemistry*; D012997:Solvents; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D010851:Picrates; D010938:Plant Oils; D012997:Solvents; D013451:Sulfonic Acids; D002245:Carbon Dioxide; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl