title: Effects of ortho-dihydroxyisoflavone derivatives from Korean fermented soybean paste on melanogenesis in B16 melanoma cells and human skin equivalents.
abstract: In this study we investigated the inhibitory effects and possible mechanisms of action of 8'-hydroxydaidzein and 3'-hydroxydaidzein, two ortho-dihydroxyisoflavone derivatives from Korean fermented soybean paste, on melanogenesis in B16 murine melanoma cells. The two hydroxydaidzeins reduced melanin synthesis comparably to treatment with kojic acid, a proven whitening agent, in B16 melanoma cells. Furthermore, when in vitro human skin equivalents were treated with the hydroxydaidzeins, the levels of melanogenesis were significantly reduced relative to a kojic acid control. The RT-PCR results demonstrated that depigmentation was due to transcriptional repression of several melanogenesis genes, including microphthalmia-associated transcription factor (MITF), by the hydroxydaidzeins. The immunoblotting results confirmed that diminution of MITF expression subsequently decreased expression of tyrosinase, and tyrosinase-related proteins 1 and 2. Cumulatively, these results suggest that hydroxydaidzeins would be potent attenuators of melanin synthesis as well as effective inhibitors of hyperpigmentation in human skin.
authors.affiliation: Skin Research Institute, AmorePacific R&D Center, Gyeonggi-do, Republic of Korea.
authors.forename: Myeong-Jin
authors.initials: MJ
authors.lastname: Goh
authors.forename: Jun-Seong
authors.initials: JS
authors.lastname: Park
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Bae
authors.forename: Duck-Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Han-Kon
authors.initials: HK
authors.lastname: Kim
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Na
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D004789:Enzyme Activation; D005285:Fermentation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017495:Hyperpigmentation / Q000378:metabolism / Q000473:pathology; D007529:Isoflavones / Q000494:pharmacology*; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D011753:Pyrones / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013025:Glycine max / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: C538958:3'-hydroxydaidzein; C498104:8-hydroxydaidzein; D000972:Antineoplastic Agents, Phytogenic; D007529:Isoflavones; C497234:MITF protein, human; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D011753:Pyrones; C011890:kojic acid; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1