title: Practical synthesis and characterization of mannose-modified chitosan.
abstract: A new and practical laboratory approach to synthesize mannose modified chitosan (Man-chitosan) was developed via reductive amination reaction. Chitosan and mannose were used as raw materials. The reaction condition was mild and controllable. The overall yield was 47-52%. Each reaction products and Man-chitosan were characterized by (1)H NMR, ESI-MS, FT-IR and TGA spectrum. FT-IR and (1)H NMR results showed that mannose conjugated to chitosan via an alkane chain bridge (CH(2)CH(2)). The degree of substitution was calculated by element analysis. TGA results indicated that mannose grafted to chitosan slightly decreased the thermal stability of chitosan in some extent. MTT assay indicated that Man-chitosan was low cytotoxicity against HepG-2 and SMMC-7721 cells.
authors.affiliation: School of Pharmacy, China Pharmaceutical University, Nanjing 210009, PR China. yaowenjun658@sina.com
authors.forename: Wenjun
authors.initials: W
authors.lastname: Yao
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiao
authors.forename: Juan
authors.initials: J
authors.lastname: Luo
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Du
authors.forename: Li
authors.initials: L
authors.lastname: Zong
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D008358:Mannose / Q000737:chemistry*; D013057:Spectrum Analysis; D013696:Temperature
chemical_list: D048271:Chitosan; D008358:Mannose