title: Cytotoxic effects of nasal buserelin on nasal mucosal tissue in rabbits.
abstract: To investigate the cytotoxic effects of nasal buserelin on rabbit nasal mucosal tissue, twenty-four female rabbits were studied prospectively. The rabbits were divided into 4 groups including 6 rabbits. The rabbits' left noses were included in the all study groups: 150 μg/puff/day of buserelin acetate was administered topically twice daily during 21, 42 and 63 days. Saline was administered topically twice daily to the left nasal cavity in the control group. The nasal septal mucosal stripe tissue was carefully removed from underlaying cartilage after sedation. HE staining, Masson's trichrome, toluidine blue and TUNEL staining were used to evaluate mucosal changes. Each preparation was investigated via apoptotic cells, and they were accounted. Kruskal-Wallis test was used to evaluate nonparametric comparison of apoptotic cells. Mononuclear cells have been raised in the sub-epithelial connective tissue, nucleuses of epithelial cells in the apical region were pyknotic, and apoptotic cells were determined on 21-day group. In the 42-day group, nasal epithelial tissue was similar to 21-day group and epithelial cells including pyknotic nucleus were present in this group, too. In the 63-day group, epithelial cells were light colored. Venous sinuses in the sub-epithelial connective tissue were wide but not congested and not raised collagen filaments. In the intra-epithelial tissue, some of cells were TUNEL (+). Apoptotic cells were fewer in the control group according to 21-day group. In 42- and 63-day groups, these cells were fewer than in 21-day group. Numerical difference was present between the groups, but statistical significance was not found between the groups. We concluded that nasal buserelin cytotoxicity was not potent in the nasal cavity in rabbits. We use nasal buserelin in all indications with confidence.
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Dumlupinar University, DPU Merkez Kampus, Tavsanli Yolu 10. Km, Tip Fakultesi, KBB AD, 43270 Kutahya, Turkey. fatihoghan@hotmail.com
authors.forename: Fatih
authors.initials: F
authors.lastname: Oghan
authors.forename: Tayfun
authors.initials: T
authors.lastname: Apuhan
authors.forename: Hakan
authors.initials: H
authors.lastname: Terzi
authors.forename: Aysel
authors.initials: A
authors.lastname: Kukner
authors.forename: Hakan
authors.initials: H
authors.lastname: Coksuer
authors.forename: Fahrettin
authors.initials: F
authors.lastname: Yılmaz
mesh_terms: D000042:Absorption; D000287:Administration, Topical; D000818:Animals; D002064:Buserelin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005260:Female; D005300:Fertility Agents, Female / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000473:pathology; D023421:Models, Animal; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism; D009300:Nasal Septum / Q000473:pathology; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects; D018675:Toxicity Tests; D014680:Veins / Q000187:drug effects / Q000473:pathology
chemical_list: D005300:Fertility Agents, Female; D002064:Buserelin