title: Specific targeting of Ep-CAM in various carcinomas by novel monoclonal antibodies.
abstract: Epithelial cell adhesion molecule (Ep-CAM) is a 40 kDa transmembrane glycoprotein overexpressed in majority of tumor epithelial cells and has a major morphoregulatory function, relevant not only to epithelial tissue development, but also in carcinogenesis and tumor progression. Since Ep-CAM localizes at the cell surface of most carcinomas, the molecule is an attractive target for immunotherapy and several strategies have been deployed to treat cancer using Ep-CAM targeting, including MAb therapy. For improving effective targeting of this protein for diagnostics in various clinical samples, we generated and characterized an anti-Ep-CAM MAb (C4) using recombinant Ep-CAM protein, comprising the highly immunogenic domain. The specificity of C4-MAb was characterized in Ep-CAM positive cell lines (PC3 and MCF-7) by flow cytometry and immunofluorescence. The immunohistochemistry analysis in clinical tissue samples showed specific detection of epithelial antigens in breast, colon, stomach, and prostate carcinomas. Thus, this Ep-CAM MAb (C4-MAb) could be used for both diagnostic and therapeutic applications due to its specificity.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India.
authors.forename: Devilakshmi
authors.initials: D
authors.lastname: Sithambaram
authors.forename: Shanthi
authors.initials: S
authors.lastname: Palanivelu
authors.forename: Krishnakumar
authors.initials: K
authors.lastname: Subramanian
authors.forename: Sanjeeb
authors.initials: S
authors.lastname: Sahoo
authors.forename: Rama S
authors.initials: RS
authors.lastname: Verma
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D020298:U937 Cells
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D003470:Culture Media; D000071858:Epithelial Cell Adhesion Molecule; D011994:Recombinant Proteins