title: Monoclonal antibodies against recombinant histidine-rich protein 2 of Plasmodium falciparum and their use in malaria diagnosis.
abstract: Histidine-rich protein-2 (HRPII) secreted by Plasmodium falciparum finds its use as a compelling marker in malaria diagnosis and follow-up. Monoclonal antibodies (MAbs) against P. falciparum HRPII are widely used in antibody-based diagnostic systems to detect HRPII protein in blood of malaria-suspected individuals. In this study, a set of five monoclonal antibodies against recombinant HRPII (rHRPII) were generated and assessed for their potential in diagnostics. Three among the five generated MAbs were of IgG1 isotype and the remaining were of IgM isotype. Probing the MAbs against proved P. falciparum infected serum and pooled control sera by immunoblotting revealed that the MAbs were successful in exposing malarial infection. Collectively, the generated MAbs have the potential to be used in immuno-based diagnostic systems uncovering P. falciparum infections.
authors.affiliation: Division of High Containment Facility, Defence Research and Development Establishment, Gwalior, Madhya Pradesh, India.
authors.forename: Samuel
authors.initials: S
authors.lastname: Merwyn
authors.forename: Natarajan
authors.initials: N
authors.lastname: Gopalan
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Ganga Prasad
authors.initials: GP
authors.lastname: Rai
authors.forename: Gauri Shanker
authors.initials: GS
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000235:genetics / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D003470:Culture Media; C514457:HRP-2 antigen, Plasmodium falciparum; D007074:Immunoglobulin G; D015800:Protozoan Proteins; D011994:Recombinant Proteins