title: Glucose sensing by time-resolved fluorescence of sol-gel immobilized glucose oxidase.
abstract: A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM.
authors.affiliation: CNISM-Dipartimento Scienze Fisiche, Università di Napoli Federico II, Napoli, Italy. rosesp@na.infn.it
authors.forename: Rosario
authors.initials: R
authors.lastname: Esposito
authors.forename: Bartolomeo
authors.initials: B
authors.lastname: Della Ventura
authors.forename: Sergio
authors.initials: S
authors.lastname: De Nicola
authors.forename: Carlo
authors.initials: C
authors.lastname: Altucci
authors.forename: Raffaele
authors.initials: R
authors.lastname: Velotta
authors.forename: Damiano Gustavo
authors.initials: DG
authors.lastname: Mita
authors.forename: Maria
authors.initials: M
authors.lastname: Lepore
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D005453:Fluorescence; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D044367:Phase Transition
chemical_list: D004800:Enzymes, Immobilized; D005182:Flavin-Adenine Dinucleotide; D005949:Glucose Oxidase; D005947:Glucose