title: Confocal microscopy imaging of NR2B-containing NMDA receptors based on fluorescent ifenprodil-like conjugates.
abstract: We describe the synthesis and pharmacological characterization of a first generation of ifenprodil conjugates 4-7 as fluorescent probes for the confocal microscopy imaging of the NR2B-containing NMDA receptor. The fluorescein conjugate 6 displayed a moderate affinity for NMDAR but a high selectivity for the NR2B subunit and its NTD. Fluorescence imaging of DS-red labeled cortical neurons showed an exact colocalization of the probe 6 with small protrusions along the dendrites related to a specific binding on NR2B-containing NMDARs.
authors.affiliation: CNRS UMR6232, Université de Caen-Basse Normandie, Cyceron, Boulevard Henri Becquerel, Caen Cedex, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Marchand
authors.forename: Javier
authors.initials: J
authors.lastname: Becerril-Ortega
authors.forename: Laetitia
authors.initials: L
authors.lastname: Mony
authors.forename: Cédric
authors.initials: C
authors.lastname: Bouteiller
authors.forename: Pierre
authors.initials: P
authors.lastname: Paoletti
authors.forename: Olivier
authors.initials: O
authors.lastname: Nicole
authors.forename: Louisa
authors.initials: L
authors.lastname: Barré
authors.forename: Alain
authors.initials: A
authors.lastname: Buisson
authors.forename: Cécile
authors.initials: C
authors.lastname: Perrio
mesh_terms: D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D010880:Piperidines / Q000032:analysis* / Q000737:chemistry*; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis* / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D005456:Fluorescent Dyes; C121001:NR2B NMDA receptor; D010880:Piperidines; D016194:Receptors, N-Methyl-D-Aspartate; C010739:ifenprodil