title: [Study on liver injury of Oncomelania hupensis caused by Eomecon chinanthe sanguinarine].
abstract: OBJECTIVE
  To analyze the effects of Eomecon chinanthe sanguinarine (SAN) on glucogen, enzyme activity and lipid peroxidation of Oncomelania hupensis liver so as to explore the mechanism of SAN against Oncomelania hupensis.
  
  
  METHODS
  SAN was extracted and purified from the dry powder of Eomecon chionantha. Oncomelania hupensis were immersed in 5 mg/L sanguinarine (50 Oncomelania hupensis per 500 ml solution) or clean water at 25 degrees C for 36 h, the livers were isolated from live snails. Total glucogen content, malondialdehyde (MDA) level, activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD), peroxidase (POD) were determined respectively and the data were analyzed by independent t test.
  
  
  RESULTS
  The glucogen content of snail livers in the SAN group and the control group were (12.151 +/- 0.204) and (18.113 +/- 0.163) mg/g respectively, the difference between the two groups was significant (P < 0.05); the MDA levels of the two groups were (5.298 +/- 0.441) and (4.351 +/- 0.197) nmol/mgprot respectively, and the difference was not significant (P > 0.05); the activities of ALT, AST, ACP, AKP, SOD in the SAN group were (2.760 +/- 0.076) U/mgprot, (68.723 +/- 2.295) U/mgprot, (407.949 +/-19.868) U/gprot, (191.287 +/- 0.771) U/ gprot and (48.452 +/- 0.193) U/mgprot respectively, the activities of these enzymes in the control group were (1.104 +/- 0.000) U/mgprot, (49.448 +/- 1.626) U/mgprot, (344.475 +/- 30.186) U/gprot, (121.905 +/- 3.127) U/gprot and (38.814 +/- 2.765) U/mgprot respectively, the activities of ALT, AST, ACP, AKP and SOD were significantly increased after immersed in 5 mg/L SAN for 36 h, the differences were significant (All P values < 0.05); yet the difference of POD between the SAN group [(22.170 +/- 0.018) U/mgprot] and the control group [(21.747 +/- 0.264) U/mgprot] was not significant (P > 0.05).
  
  
  CONCLUSIONS
  SAN can destroy liver functions of Oncomelania hupensis through decreasing glucogen content and changing activities of some important enzymes in snail liver.
authors.affiliation: Department of Laboratory Medicine, Medical College in Hunan Normal University, Changsha 410013, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Sun
authors.forename: Shi-Shan
authors.initials: SS
authors.lastname: Yuan
authors.forename: Qiong-Yao
authors.initials: QY
authors.lastname: Huang
authors.forename: Fei
authors.initials: F
authors.lastname: Peng
authors.forename: Nian-Meng
authors.initials: NM
authors.lastname: Liu
authors.forename: Sheng-Qing
authors.initials: SQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D053119:Benzophenanthridines / Q000302:isolation & purification / Q000494:pharmacology*; D004798:Enzymes / Q000187:drug effects / Q000378:metabolism; D006003:Glycogen / Q000032:analysis; D006801:Humans; D007546:Isoquinolines / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000032:analysis; D028101:Papaveraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012552:Schistosomiasis / Q000517:prevention & control*; D012908:Snails / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D053119:Benzophenanthridines; D004798:Enzymes; D007546:Isoquinolines; D010936:Plant Extracts; D008315:Malondialdehyde; D006003:Glycogen; C005705:sanguinarine