title: Bone marrow of multiorgan donors underutilized:  implications for improvement of accessibility of hematopoietic cells for transplantations.
abstract: BACKGROUND
  The demand for human hematopoietic stem and progenitor cells (HSPCs) for transplantation is increasing. Thus, effective alternative sources of HSPCs are required. Consequently, we sought to expand the accessibility of hematopoietic cells for clinical purposes by the investigation of hematopoietic reconstitution after transplantation of human HSPCs harvested from the bone marrow (BM) of heparinized deceased organ donors (HDODs).
  
  
  METHODS
  For multipart research comparison, human BM HDODs-, healthy donor-derived, umbilical cord blood nuclear cells, or CD34(+) cells were transplanted into sublethally irradiated NOD/SCID mice. Twenty-eight days after transplantation nuclear cells were isolated from the murine BM, spleen, and peripheral blood and were used to quantitatively detect human CD45 antigen by quantitative real-time reverse transcriptase-polymerase chain reaction and flow cytometry. The clonogenic growth of human colony-forming units was also investigated.
  
  
  RESULTS
  We found that umbilical cord blood-derived HSPCs showed the greatest transplantation potential in our in vivo model. Interestingly, the transplantation potential of HSPCs collected from the BM of HDODs was of the same quality as cells obtained from healthy BM donors.
  
  
  CONCLUSIONS
  Based on these results, we conclude that HDODs are a strongly underappreciated source of HSPCs that are ready to use for clinical purposes.
authors.affiliation: Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Baumert
authors.forename: Miłosz P
authors.initials: MP
authors.lastname: Kawa
authors.forename: Maciej
authors.initials: M
authors.lastname: Kotowski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Grymuła
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pabisiak
authors.forename: Ewa
authors.initials: E
authors.lastname: Pius
authors.forename: Jarosław
authors.initials: J
authors.lastname: Peregud-Pogorzelski
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Walczak
authors.forename: Marek
authors.initials: M
authors.lastname: Ostrowski
authors.forename: Bogusław
authors.initials: B
authors.lastname: Machaliński
mesh_terms: D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002102:Cadaver; D002469:Cell Separation / Q000379:methods; D003114:Colony-Forming Units Assay; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000706:statistics & numerical data*; D006801:Humans; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000378:metabolism; D019520:Living Donors / Q000600:supply & distribution; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D012042:Registries; D014015:Tissue Banks; D014019:Tissue Donors / Q000600:supply & distribution; D009927:Tissue and Organ Procurement / Q000706:statistics & numerical data*; D018183:Transplantation Chimera / Q000276:immunology; D014183:Transplantation, Heterologous
chemical_list: D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human