title: [Comparative immunogenicity studies of cultural and peptide influenza vaccines].
abstract: OBJECTIVE
  Comparative immunogenicity studies of experimental vaccines based onA/Aichi/2/68 neuraminidase peptide fragments (NA) and influenza virus A and B strains produced in MDCK cell culture.
  
  
  METHODS
  Anti-hemagglutinin and virus neutralizing activity of mice sera was determined in MN and HI reactions in accordance with the WHO recommendations.
  
  
  RESULTS
  Sera against peptides 136-147 and 154-164 from variable sites, as well as against peptide 314-328 from conservative region of the heavy chain of A/ Aichi/2/68 influenza virus NA showed distinct anti-hemagglutinin and neutralizing activity against homologous influenzavirus. Anti-(314-328) serum was also active in HI and MN reactions against other strains of the H3N2 subtype. Combined administration of peptide sample with an immunomodulator (Immunomax) increased the immunogenicity to the level of the cultural samples based on influenza A virus.
  
  
  CONCLUSIONS
  The results show higher immunogenicity of cultural vaccines based on influenza virus in comparison to peptide samples. A possibility of peptide vaccine immunogenicity increase was demonstrated by combined administration with the immunomodulator.
authors.forename: E I
authors.initials: EI
authors.lastname: Isaeva
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: R Ia
authors.initials: RIa
authors.lastname: Podcherniaeva
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000276:immunology; D006801:Humans; D055261:Immunogenetic Phenomena; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007668:Kidney / Q000166:cytology; D051379:Mice; D009439:Neuraminidase / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D044967:Serum / Q000276:immunology / Q000821:virology; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D010446:Peptide Fragments; D022223:Vaccines, Subunit; D009439:Neuraminidase