title: Adenovirus-based oral vaccine for rabbit hemorrhagic disease.
abstract: Vaccine antigens for rabbit hemorrhagic disease virus (RHDV) are currently derived from inactivated RHDV obtained from the livers of experimentally infected rabbits or from several recombinant immunogens. However, the application of these vaccine antigens has been restricted because of biosecurity and immunity characteristics. In the current study, a recombinant adenovirus expressing the RHDV capsid protein (VP60) was constructed and the expression of the recombinant protein was identified through western blot analysis using RHDV-positive rabbit sera. Eighteen rabbits were immunized by injection, direct oral instillation, or using bait. They were challenged with RHDV isolate three weeks after boost immunization. In all cases, the rabbits immunized with the recombinant adenovirus developed RHDV-specific antibodies and cell immune response. The rabbits injected with the recombinant adenovirus were completely protected against RHDV challenge. The adenovirus expression system may provide a strategy for the immunization of rabbits, particularly for the control of RHDV in wild rabbits.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Qiu
authors.forename: Huafang
authors.initials: H
authors.lastname: Hao
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Fu
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhang
authors.forename: Shengfang
authors.initials: S
authors.lastname: He
authors.forename: Shuxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Enqi
authors.initials: E
authors.lastname: Du
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae; D000284:Administration, Oral; D000818:Animals; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D036022:Capsid Proteins / Q000276:immunology; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral; D011817:Rabbits / Q000276:immunology / Q000821:virology; D014614:Vaccines, Synthetic; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D036022:Capsid Proteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines