title: Protective effect of Lactobacillus rhamnosus 231 against N-Methyl-N'-nitro-N-nitrosoguanidine in animal model.
abstract: The protective effect of Lactobacillus rhamnosus 231 (Lr 231) against potent carcinogen N-Methyl-N'-Nitro-N-Nitrosoguanidine (MNNG) in the rat model is studied. Daily feeding with Lr 231 improved the body weight of male Wistar rats compared with control groups. Fecal azoreductase (p < 0.001) and nitroreductase (p < 0.01) enzyme activity decreased significantly in Lr 231 group in comparison with control groups that received only phosphate buffer or MNNG. Oral administration of MNNG led to a significant increase in Glutathione transferase (GST) while Glutathione reductase (GSH) showed decreased activity. Conversely, feeding Lr 231 showed significantly increased GSH and decreased GST activity in comparison to the MNNG group, emphasizing the protection provided by Lr 231 against MNNG. Histopathological analysis of liver, spleen and colon showed decreased signs of inflammation in the Lr 231 group. The present study highlights that inclusion of active Lr 231 in regular diets could be used to prevent MNNG induced colon carcinoma.
authors.affiliation: Department of Pharmacy, Saurashtra University, Rajkot, India.
authors.forename: Viral
authors.initials: V
authors.lastname: Gosai
authors.forename: Padma
authors.initials: P
authors.lastname: Ambalam
authors.forename: Maya
authors.initials: M
authors.lastname: Raman
authors.forename: C R
authors.initials: CR
authors.lastname: Kothari
authors.forename: R K
authors.initials: RK
authors.lastname: Kothari
authors.forename: B R M
authors.initials: BR
authors.lastname: Vyas
authors.forename: Navin R
authors.initials: NR
authors.lastname: Sheth
mesh_terms: D000818:Animals; D001835:Body Weight; D003106:Colon / Q000201:enzymology / Q000473:pathology; D004789:Enzyme Activation; D057075:Enzyme Assays; D005243:Feces / Q000201:enzymology; D005982:Glutathione Transferase / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000009:adverse effects*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D019936:Probiotics / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology / Q000473:pathology
chemical_list: D008769:Methylnitronitrosoguanidine; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase; D005982:Glutathione Transferase