title: Detection of single-nucleotide polymorphism on uidA gene of Escherichia coli by a multiplexed electrochemical DNA biosensor with oligonucleotide-incorporated nonfouling surface.
abstract: We report here a practical application of a multiplexed electrochemical DNA sensor for highly specific single-nucleotide polymorphism (SNP) detection. In this work, a 16-electrode array was applied with an oligonucleotide-incorporated nonfouling surfaces (ONS) on each electrode for the resistance of unspecific absorption. The fully matched target DNA templated the ligation between the capture probe assembled on gold electrodes and the tandem signal probe with a biotin moiety, which could be transduced to peroxidase-based catalyzed amperometric signals. A mutant site (T93G) in uidA gene of E. coli was analyzed in PCR amplicons. 10% percentage of single mismatched mutant gene was detected, which clearly proved the selectivity of the multiplexed electrochemical DNA biosensor when practically applied.
authors.affiliation: Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China. liug@simt.com.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Ruojun
authors.initials: R
authors.lastname: Lao
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.forename: Lanying
authors.initials: L
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.forename: Sanjay
authors.initials: S
authors.lastname: Mathur
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D029968:Escherichia coli Proteins / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics*; D006046:Gold / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
chemical_list: D029968:Escherichia coli Proteins; D009841:Oligonucleotides; D001710:Biotin; D006046:Gold; D004247:DNA; D009195:Peroxidase; D005966:Glucuronidase