title: Chemically inhibited ATP synthesis promoted detachment of different-age biofilms from membrane surface.
abstract: This study investigated the response of different-age biofilms developed on membrane surface to a chemical uncoupler 3, 3', 4', 5-tetrachlorosalicylanilide (TCS). Results showed that adenosine triphosphate (ATP) dissipation caused by TCS would promote different-age biofilms detachment, whereas chemically inhibited cellular ATP synthesis subsequently suppressed autoinducer-2 (AI-2) and extracellular polymeric substances (EPS) production. The extent of biofilm detachment was found to be closely related to AI-2-regulated EPS contents of bacteria. It was revealed that energy dissipation induced biofilm detachability was controlled by AI-2 regulated cellular communication via AI-2-mediated EPS secretion. This study would lead to a new cleaning strategy of biologically fouled membrane.
authors.affiliation: Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Xu
authors.forename: Kaiwen
authors.initials: K
authors.lastname: Teo
authors.forename: Huiling
authors.initials: H
authors.lastname: Neo
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms; D002462:Cell Membrane; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; D000255:Adenosine Triphosphate