title: Metals in biology: defining metalloproteomes.
abstract: The vital nature of metal uptake and balance in biology is evident in the highly evolved strategies to facilitate metal homeostasis in all three domains of life. Several decades of study on metals and metalloproteins have revealed numerous essential bio-metal functions. Recent advances in mass spectrometry, X-ray scattering/absorption, and proteomics have exposed a much broader usage of metals in biology than expected. Even elements such as uranium, arsenic, and lead are implicated in biological processes as part of an emerging and expansive view of bio-metals. Here we discuss opportunities and challenges for established and newer approaches to study metalloproteins with a focus on technologies that promise to rapidly expand our knowledge of metalloproteins and metal functions in biology.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States. SMYannone@lbl.gov
authors.forename: Steven M
authors.initials: SM
authors.lastname: Yannone
authors.forename: Sophia
authors.initials: S
authors.lastname: Hartung
authors.forename: Angeli L
authors.initials: AL
authors.lastname: Menon
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: John A
authors.initials: JA
authors.lastname: Tainer
mesh_terms: D001105:Archaea / Q000737:chemistry; D001419:Bacteria / Q000737:chemistry; D017753:Ecosystem; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008667:Metalloproteins / Q000032:analysis* / Q000737:chemistry; D008670:Metals / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D020543:Proteome / Q000032:analysis*
chemical_list: D008667:Metalloproteins; D008670:Metals; D020543:Proteome