title: Identification and expression pattern of ribosomal L5 gene in common wheat (Triticum aestivum L.).
abstract: The full-length cDNA sequence (1158 bp) encoding a ribosomal L5 protein, designated as TaL5, was firstly isolated from common wheat (Triticum aestivum L.) using the rapid amplification of cDNA ends method (RACE). The open reading frame (ORF) of TaL5 gene was 906 bp, and its deduced amino acid sequence (301 residues) shared high similarity to those of other higher plant L5 proteins. TaL5 protein contained a putative 5S binding region (74 amino acids). TaL5 DNA sequence was further cloned, and sequence analysis showed that it contained 7 introns and 8 exons. Predicated using TargetP software, TaL5 protein was putatively located in mitochondria and contains a transit peptide of 12 amino acids. During grain filling period, temporal expression pattern of TaL5 gene was approximately consistent with the rates of starch accumulation in grains. Additionally, TaL5 gene was dramatically induced by salt, drought and freezing stresses, exogenous abscisic acid (ABA) and salicylic acid (SA) in wheat seedlings. These implied that TaL5 gene could function in growth, development and abiotic stresses in wheat plants.
authors.affiliation: National Engineering Research Centre for Wheat, Henan Agricultural University, 450002 Zhengzhou, People's Republic of China. zkangg@163.com
authors.forename: Guo-Zhang
authors.initials: GZ
authors.lastname: Kang
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Peng
authors.forename: Qiao-Xia
authors.initials: QX
authors.lastname: Han
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Tian-Cai
authors.initials: TC
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017343:Genes, Plant; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D013315:Stress, Psychological; D014908:Triticum / Q000235:genetics*
chemical_list: D012269:Ribosomal Proteins