title: Determination of the activity of CD134 (OX-40) and CD137 (4-1BB) adhesive molecules by means of flow cytometry in patients with colorectal cancer metastases to the liver.
abstract: Colorectal carcinoma (CRC) is one of the most common reasons of mortality in patients diagnosed with neoplasms. In nearly 20% of patients with colorectal carcinoma metastatic lesions are diagnosed. In general, survival of patients with metastatic lesions to the liver and other organs is poor. Conventional therapy of colorectal carcinoma is based on the surgical excision of the tumor, chemotherapy, and radiotherapy.
  
  
  OBJECTIVE
  was to determine the expression of CD134 and CD137 molecules inside the tumor, at the border of the tumor, in the healthy tissue, and peripheral blood, considering patients with colorectal carcinoma metastases to the liver.
  
  
  METHODS
  The study group comprised 39 patients subject to surgical treatment at the Department of General and Gastroenterological Surgery, due to colorectal carcinoma with liver metastases. CD134 and CD137 adhesive molecule levels were determined inside the tumor, at the border of the tumor, and in the healthy margins of the surgical incision. Additionally, the authors evaluated the peripheral blood level of the above-mentioned molecules on the day of the surgical procedure, and 10 days, thereafter.
  
  
  RESULTS
  The mean CD134 levels were the highest inside the tumor, significantly decreasing towards the direction of healthy tissues. The average peripheral blood molecule levels were four-fold higher on the day of the surgical procedure, as compared to values obtained on the tenth postoperative day. This dependency also concerned the remaining statistical measures.The mean CD137 levels showed no significant difference, regardless their location. The authors observed significant, peripheral blood, CD137 level differences, considering the day of the surgical procedure and tenth postoperative period. The mean CD137 peripheral blood level was several times higher on the day of the surgical procedure, as compared to the postoperative period.
  
  
  CONCLUSIONS
  The determination of the activity of CD134 and CD137 molecules might create opportunities to plan treatment and predict prognosis in case of colorectal carcinoma. Proper immuno-therapeutic management which is based on the expression of the above-mentioned molecules might help determine the risk of metastases, preventing from their development. In advanced cases treatment of liver metastases might be possible.
authors.affiliation: 2nd Department of General and Gastroenterological Surgery, Medical University in Białystok.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Cepowicz
authors.forename: Konrad
authors.initials: K
authors.lastname: Zaręba
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gryko
authors.forename: Anna
authors.initials: A
authors.lastname: Stasiak-Bermuta
authors.forename: Bogusław
authors.initials: B
authors.lastname: Kędra
mesh_terms: D053330:4-1BB Ligand / Q000032:analysis* / Q000097:blood; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D015179:Colorectal Neoplasms / Q000097:blood / Q000737:chemistry* / Q000175:diagnosis / Q000556:secondary*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D057234:Preoperative Period; D053262:Receptors, OX40 / Q000032:analysis* / Q000097:blood; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000032:analysis* / Q000097:blood
chemical_list: D053330:4-1BB Ligand; D014408:Biomarkers, Tumor; D053262:Receptors, OX40; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9