title: SNaPshot® minisequencing analysis of multiple ancestry-informative Y-SNPs using capillary electrophoresis.
abstract: This protocol describes a strategy for analyzing phylogenetic Y-SNPs in a hierarchical multiplex assay by utilizing the SNaPshot(®) Multiplex System. Step by step, the protocol assists in the appropriate selection of SNPs, the primer design, the set up of PCR/SBE reactions as well as in the analysis of the results. Furthermore, a forensic approach is highlighted, in which the most probable ancestry of an unknown male DNA is inferred by the geographical distribution of the assigned Y-SNP haplogroup.
authors.affiliation: Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité-Universitätsmedizin, Berlin, Germany. maria.geppert@charite.de
authors.forename: Maria
authors.initials: M
authors.lastname: Geppert
authors.forename: Lutz
authors.initials: L
authors.lastname: Roewer
mesh_terms: D020029:Base Pairing / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D005789:Genealogy and Heraldry; D005843:Geography; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013223:Statistics as Topic
chemical_list: D017931:DNA Primers