title: Evaluation of Royal Sun Agaricus, Agaricus brasiliensis S. Wasser et al., aqueous extract in mice challenged with Salmonella enterica serovar Typhimurium.
abstract: This study investigated the effects of Agaricus brasiliensis S. Wasser et al. (=Agaricus blazei Murrill sensu Heinem.) aqueous extract on small intestinal sIgA levels, serum TNF-alpha, IFN-gamma and IL-10 levels, splenic index, bacterial translocation, and histology of small intestine, spleen, and liver from mice orally challenged with 10(6) CFU of Salmonella enterica serovar Typhimurium (SEST). Splenic index values as well as sIgA, TNF-alpha, IFN-gamma, and IL-10 levels were not affected by either A. brasiliensis aqueous extract treatment or by pathogenic challenge. Typical colonies of SEST were recovered from liver, spleen, and mesenteric lymph nodes of challenged animals, but there was no significant difference in this translocation between groups treated or not with A. brasiliensis aqueous extract. Translocation was confirmed by histopathological analysis in mice challenged with SEST, which showed small and diffuse foci of mixed inflammatory infiltrate in hepatic parenchyma. In conclusion, A. brasiliensis aqueous extract as tested in the present study did not influence any of the variables selected to evaluate in vivo its immunomodulatory effect suggested in the literature.
authors.affiliation: Departamento de Produção Vegetal, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, ES, Brasil. betefantuzzi@yahoo.com
authors.forename: Elisabete
authors.initials: E
authors.lastname: Fantuzzi
authors.forename: Lucilene Rezende
authors.initials: LR
authors.lastname: Anastácio
authors.forename: Jacques Robert
authors.initials: JR
authors.lastname: Nicoli
authors.forename: Sérgio Oliveira
authors.initials: SO
authors.lastname: de Paula
authors.forename: Rosa Maria Esteves
authors.initials: RM
authors.lastname: Arantes
authors.forename: Maria Cristina Dantas
authors.initials: MC
authors.lastname: Vanetti
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D018988:Bacterial Translocation; D001835:Body Weight / Q000187:drug effects; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood* / Q000187:drug effects / Q000378:metabolism; D007071:Immunoglobulin A, Secretory / Q000187:drug effects / Q000378:metabolism; D007371:Interferon-gamma / Q000097:blood / Q000187:drug effects; D016753:Interleukin-10 / Q000097:blood; D007421:Intestine, Small / Q000187:drug effects / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology*; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000187:drug effects
chemical_list: D045424:Complex Mixtures; D016207:Cytokines; D007071:Immunoglobulin A, Secretory; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma