title: [Incidence of leptospirosis in Pardubice and Hradec Králové region and a part of Vysočina region in the years 2002-2009].
abstract: BACKGROUND
  The aim of this study was to evaluate the incidence of leptospirosis in Pardubice and Hradec Králové regions and a part of Vysočina region in 2002-2009. A group of patients was statistically analyzed and the incidence rates of leptospirosis in individual months and years were calculated.
  
  
  METHODS
  A group of 4,813 patients with suspected leptospirosis from Pardubice and Hradec Králové regions and a part of Vysočina region were examinated in our centre in 2002-2009. A total of 5,366 biological samples were studied. From 4,625 blood samples, 3,958 were examinated with the serological microagglutination-lysis method and the remaining 667 with the PCR method.
  
  
  RESULTS
  From 4,813 patients suspected to have leptospirosis, 89 were found to be leptospirosis-positive. Leptospirosis was diagnosed in 63 males (70.7 %) a 26 females (29.3 %); the mean age were 39.52 ± 17.67 years in males and 46.85 ± 18.34 years in females; the mean age men + women was 41.66 ± 18.13 years (range 3-78 years). Most positive male patients (n = 28) were in 26-45 age group; the findings were similar in females (n = 10). The highest number of leptospirosis cases was noted in 2005. Most frequently, infection caused by pathogenic leptospires was diagnosed in November.
  
  
  CONCLUSIONS
  The incidence rates of leptospirosis in Pardubice and Hradec Králové regions and a part of Vysočina region in 2002-2009 (with the exception of 2008) exceeded the overall reported levels 0.4/100,000 population.
authors.affiliation: Department of Microbiology, Medical Faculty of Charles University Hradec Králové, Czech Republic. pernilla@seznam.cz
authors.forename: Petra
authors.initials: P
authors.lastname: Kučerová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Cermáková
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Valenta
authors.forename: Pavel
authors.initials: P
authors.lastname: Polák
authors.forename: Lenka
authors.initials: L
authors.lastname: Plíšková
authors.forename: Oto
authors.initials: O
authors.lastname: Pavliš
authors.forename: Barbora
authors.initials: B
authors.lastname: Voxová
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000372:Agglutination Tests; D002648:Child; D002675:Child, Preschool; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D006801:Humans; D015994:Incidence; D007922:Leptospirosis / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D055815:Young Adult