title: Differential expression of LeY and fucosyltransferase IV correlates with the receptivity of RL95-2 and HEC-1A human uterine epithelial cells.
abstract: Adhesion molecules expressed on the uterine endometrium are potential receptive markers in embryo implantation. RL95-2 and HEC-1A cell lines represent the high- and low-receptive endometrial epithelium respectively. LeY (Lewis Y) is a difucosylated oligosaccharide highly expressed in the endometrium of some species during implantation. α1, 3 fucosylation of LeY is catalysed by FUT4 (fucosyltransferase IV), the key synthesis enzyme for LeY. We investigated whether the difference in receptivity between the 2 cell lines was related to different expressions of LeY and FUT4. RL95-2 cells expressed a higher level of LeY and FUT4 than HEC-1A cells, as shown by immunofluorescent staining, RT-PCR (reverse transcription-PCR) or Western blotting. FUT4-siRNA (small interfering RNA) transfection down-regulated FUT4 and LeY in RL95-2 cells, and inhibited the adhesion of the embryonic cells (JAR) to RL95-2 cell monolayer. FUT4-cDNA, however, increased the expression of FUT4 and LeY in HEC-1A cells, and increased the adhesion of embryonic cells to HEC-1A cell monolayer. Alterations of LeY level by up- or down-regulation of FUT4 also mediated EGFR (epidermal growth factor receptor)/MAPK (mitogen-activated protein kinase) signalling pathway. To conclude, the expression of LeY and FUT4 correlates with endometrial receptivity, making them potential new markers for the evaluation of endometrial receptivity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian, Peoples Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Wei
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Wang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
mesh_terms: D002448:Cell Adhesion / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002460:Cell Line; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000201:enzymology / Q000378:metabolism; D066246:ErbB Receptors / Q000096:biosynthesis; D005260:Female; D005647:Fucosyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis / Q000378:metabolism*; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D015815:Cell Adhesion Molecules; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; D034741:RNA, Small Interfering; C066693:FUT4 protein, human; D005647:Fucosyltransferases; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases