title: Regeneration of native-like neo-urinary tissue from nonbladder cell sources.
abstract: Urinary pathology requiring urinary diversion, partial or full bladder replacement, is a significant clinical problem affecting ~14,000 individuals annually in the United States alone. The use of gastrointestinal tissue for urinary diversion or bladder reconstruction/replacement surgeries is frequently associated with complications. To try and alleviate or reduce the frequency of these complications, tissue engineering and regenerative medicine strategies have been developed using bio-absorbable materials seeded with cells derived from the bladder. However, bladder-sourced cells may not always be suitable for such applications, especially in patients with bladder cancer. In this study, we describe the isolation and characterization of smooth muscle cells (SMCs) from porcine adipose and peripheral blood that are phenotypically and functionally indistinguishable from bladder-derived SMCs. In a preclinical Good Laboratory Practice study, we demonstrate that autologous adipose- and peripheral blood-derived SMCs may be used to seed synthetic, biodegradable tubular scaffold structures and that implantation of these seeded scaffolds into a porcine cystectomy model leads to successful de novo regeneration of a tubular neo-organ composed of urinary-like neo-tissue that is histologically identical to native bladder. The ability to create urologic structures de novo from scaffolds seeded by autologous adipose- or peripheral blood-derived SMCs will greatly facilitate the translation of urologic tissue engineering technologies into clinical practice.
authors.affiliation: Bioprocess Research and Assay Development, Tengion Inc., Winston-Salem, North Carolina 27103, USA. joydeep.basu@tengion.com
authors.forename: Joydeep
authors.initials: J
authors.lastname: Basu
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Jayo
authors.forename: Roger M
authors.initials: RM
authors.lastname: Ilagan
authors.forename: Kelly I
authors.initials: KI
authors.lastname: Guthrie
authors.forename: Namrata
authors.initials: N
authors.lastname: Sangha
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Genheimer
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Quinlan
authors.forename: Richard
authors.initials: R
authors.lastname: Payne
authors.forename: Toyin
authors.initials: T
authors.lastname: Knight
authors.forename: Elias
authors.initials: E
authors.lastname: Rivera
authors.forename: Deepak
authors.initials: D
authors.lastname: Jain
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Bertram
authors.forename: John W
authors.initials: JW
authors.lastname: Ludlow
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D001743:Urinary Bladder / Q000601:surgery*