title: Molecular genetic analysis of Mycobacterium tuberculosis strains spread in different patient groups in St. Petersburg, Russia.
abstract: Molecular epidemiological features of Mycobacterium tuberculosis strains among different patient groups in Russia have not been studied well. The aim of our study was to compare the genotypes of M. tuberculosis strains circulating among tuberculosis (TB) patients from different groups: homeless, human immunodeficiency virus (HIV)-infected, prisoners, and the general population of St. Petersburg citizens. One hundred and forty-two M. tuberculosis complex isolates from different TB patient groups were studied using the spacer oligonucleotide typing (spoligotyping) method. The majority of the studied M. tuberculosis isolates in all groups belonged to the Beijing family (55% among homeless; 77% among HIV-infected; 60% among the general population; 81% among prisoners). There were no significant differences in the Beijing family prevalence among homeless patients, HIV/TB co-infected patients, and the general population of TB patients. The lowest genetic diversity of the pathogen was detected among imprisoned patients. The results of our study demonstrate that M. tuberculosis strains circulating among patients from high-risk groups are also spread among the general population of St. Petersburg citizens.
authors.affiliation: The Biomedical Center, 8, Vyborgskaya St., St. Petersburg, 194044, Russia. echernya@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: Chernyaeva
authors.forename: P
authors.initials: P
authors.lastname: Dobrynin
authors.forename: N
authors.initials: N
authors.lastname: Pestova
authors.forename: N
authors.initials: N
authors.lastname: Matveeva
authors.forename: V
authors.initials: V
authors.lastname: Zhemkov
authors.forename: A
authors.initials: A
authors.lastname: Kozlov
mesh_terms: D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008967:Molecular Biology; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012426:Russia / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology*