title: TraML--a standard format for exchange of selected reaction monitoring transition lists.
abstract: Targeted proteomics via selected reaction monitoring is a powerful mass spectrometric technique affording higher dynamic range, increased specificity and lower limits of detection than other shotgun mass spectrometry methods when applied to proteome analyses. However, it involves selective measurement of predetermined analytes, which requires more preparation in the form of selecting appropriate signatures for the proteins and peptides that are to be targeted. There is a growing number of software programs and resources for selecting optimal transitions and the instrument settings used for the detection and quantification of the targeted peptides, but the exchange of this information is hindered by a lack of a standard format. We have developed a new standardized format, called TraML, for encoding transition lists and associated metadata. In addition to introducing the TraML format, we demonstrate several implementations across the community, and provide semantic validators, extensive documentation, and multiple example instances to demonstrate correctly written documents. Widespread use of TraML will facilitate the exchange of transitions, reduce time spent handling incompatible list formats, increase the reusability of previously optimized transitions, and thus accelerate the widespread adoption of targeted proteomics via selected reaction monitoring.
authors.affiliation: Institute for Systems Biology, Seattle, Washington 98109, USA. edeutsch@systemsbiology.org
authors.forename: Eric W
authors.initials: EW
authors.lastname: Deutsch
authors.forename: Matthew
authors.initials: M
authors.lastname: Chambers
authors.forename: Steffen
authors.initials: S
authors.lastname: Neumann
authors.forename: Fredrik
authors.initials: F
authors.lastname: Levander
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Binz
authors.forename: Jim
authors.initials: J
authors.lastname: Shofstahl
authors.forename: David S
authors.initials: DS
authors.lastname: Campbell
authors.forename: Luis
authors.initials: L
authors.lastname: Mendoza
authors.forename: David
authors.initials: D
authors.lastname: Ovelleiro
authors.forename: Kenny
authors.initials: K
authors.lastname: Helsens
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Robert L
authors.initials: RL
authors.lastname: Moritz
authors.forename: Mi-Youn
authors.initials: MY
authors.lastname: Brusniak
mesh_terms: D007256:Information Systems; D040901:Proteomics; D012984:Software