title: Structural determination and chemical esterification of the sophorolipids produced by Candida bombicola grown on glucose and α-linolenic acid.
abstract: The extracellular surface-active glycolipids produced by the yeast, Candida bombicola when grown on glucose and α-linolenic acid, were analyzed by HPLC with electro-spray ionization (ESI-MS) and collision-induced dissociation mass spectrometry. The analysis confirmed that the sophorolipid (SL) mixture contained three different forms of C18:3 SL molecules: free acid, lactone and a diacetylated lactone, which has not been reported previously. Also a minor amount of diacetylated lactone form of C18:1 SL was detected. Further, the SL mixture was subjected to chemical esterification reaction with sodium methoxide. The reaction product was analyzed with ESI-MS and confirmed to be the single homogenous esterified product containing C18:3 moieties in its fatty acid chain.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Pune, 411008, India. reetika.reeti@gmail.com
authors.forename: Reetika
authors.initials: R
authors.lastname: Gupta
authors.forename: Asmita A
authors.initials: AA
authors.lastname: Prabhune
mesh_terms: D002175:Candida / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004951:Esterification; D005947:Glucose / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D017962:alpha-Linolenic Acid; D005947:Glucose