title: Common variants in chromosome 4q25 are associated with myopia in Chinese adults.
abstract: OBJECTIVE
  A genome-wide association study (GWAS) of high myopia found that five single nucleotide polymorphisms (SNPs) in 4q25 were associated with high myopia. The original study suggested replication studies were needed to validate these results. The aim of our study was to examine if these SNPs are associated with myopia in a different set of samples.
  
  
  METHODS
  Genomic DNA was collected from 2307 unrelated individuals, including 1052 university students without myopia and 1255 university students with myopia (615 students with moderate myopia and 640 students with high myopia). Four of the five reported SNPs at 4q25, rs2218817, rs10034228, rs1585471 and rs6837348, were genotyped using SNaPshot. The genotype and allele frequencies of the SNPs between myopias and controls were compared by chi-squared test.
  
  
  RESULTS
  Genotype and allele frequencies of the four SNPs showed significant differences between the myopia and control groups (rs2218817: genotype p = 0.009, OR = 1.18; rs10034228: genotype p = 0.009, OR = 1.18; rs1585471: genotype p = 0.007, OR = 1.19 and rs6837348: genotype p = 0.012, OR = 1.18).
  
  
  CONCLUSIONS
  Our results support the association between the four SNPs and myopia, suggesting a myopia predisposing locus at 4q25 that deserves further study.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Panfeng
authors.initials: P
authors.lastname: Wang
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Li
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xiao
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Jia
authors.forename: Xiangming
authors.initials: X
authors.lastname: Guo
authors.forename: Qingjiong
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People / Q000235:genetics*; D002681:China; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008297:Male; D009216:Myopia / Q000235:genetics*; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult