title: The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits.
abstract: OBJECTIVE
  To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC).
  
  
  METHODS
  Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15 min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48 h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell.
  
  
  RESULTS
  The animals of GII and GII-24h-48 h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48 h showed lower thickness of alveolar cells that GI and GI-24h-48 h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group.
  
  
  CONCLUSIONS
  N-acetyl-cysteine administered 15 min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
authors.affiliation: Laboratory Surgical Technique, Federal University of Rio Grande, Brazil. angelapotterc@hotmail.com
authors.forename: Angela Potter de
authors.initials: AP
authors.lastname: Castro
authors.forename: Miguel Angelo Martins de
authors.initials: MA
authors.lastname: Castro Junior
authors.forename: Susi
authors.initials: S
authors.lastname: Lauz
authors.forename: Emilio
authors.initials: E
authors.lastname: Facin
authors.forename: Manuel de Jesus
authors.initials: Mde J
authors.lastname: Simões
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D005947:Glucose / Q000008:administration & dosage; D007262:Infusions, Intravenous; D008099:Liver / Q000098:blood supply*; D055370:Lung Injury / Q000517:prevention & control*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; D005947:Glucose; D000111:Acetylcysteine