title: Brenneria goodwinii sp. nov., associated with acute oak decline in the UK.
abstract: A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus Brenneria, family Enterobacteriaceae, and are most closely related to Brenneria rubrifaciens (97.6 % sequence similarity to the type strain). Multilocus sequence analysis based on four housekeeping genes (gyrB, rpoB, infB and atpD) confirmed their position within the genus Brenneria, while DNA-DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phenotypically from their closest phylogenetic neighbours. The phylogenetic and phenotypic data demonstrate that these isolates from oak with symptoms of AOD represent a novel species in the genus Brenneria, for which the name Brenneria goodwinii sp. nov. (type strain FRB 141(T)  = R-43656(T)  = BCC 845(T)  = LMG 26270(T)  = NCPPB 4484(T)) is proposed.
authors.affiliation: Forest Research, Centre for Forestry and Climate Change, Alice Holt Lodge, Farnham, Surrey GU10 4LH, UK.
authors.forename: Sandra
authors.initials: S
authors.lastname: Denman
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Carrie
authors.initials: C
authors.lastname: Brady
authors.affiliation: Forest Research, Centre for Forestry and Climate Change, Alice Holt Lodge, Farnham, Surrey GU10 4LH, UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Kirk
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.affiliation: Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
authors.forename: Stephanus
authors.initials: S
authors.lastname: Venter
authors.affiliation: Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
authors.forename: Teresa
authors.initials: T
authors.lastname: Coutinho
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D029963:Quercus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D006113:United Kingdom
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S