title: Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells.
abstract: Chondroitin sulfates (CSs) and dermatan sulfates (DSs) are enriched in the microenvironment of neural stem cells (NSCs) during development and in the adult neurogenic niche, and have been implicated in mechanisms governing neural precursor migration, proliferation and differentiation. In contrast to previous studies, in which a chondroitinaseABC-dependent unselective deglycosylation of both CSs and DSs was performed, we used chondroitin 4-O-sulfotransferase-1 (Chst11/C4st1)- and dermatan 4-O-sulfotransferase-1 (Chst14/D4st1)-deficient NSCs specific for CSs and DSs, respectively, to investigate the involvement of specific sulfation profiles of CS and DS chains, and thus the potentially distinct roles of CSs and DSs in NSC biology. In comparison to wild-type controls, deficiency for Chst14 resulted in decreased neurogenesis and diminished proliferation of NSCs accompanied by increased expression of GLAST and decreased expression of Mash-1, and an upregulation of the expression of the receptors for fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). By contrast, deficiency in Chst11 did not influence NSC proliferation, migration or differentiation. These observations indicate for the first time that CSs and DSs play distinct roles in the self-renewal and differentiation of NSCs.
authors.affiliation: Zentrum für Molekulare Neurobiologie, Universitätskrankenhaus Hamburg-Eppendorf, Universität Hamburg, Martinistr. 52, D-20246 Hamburg,  Germany.
authors.forename: Shan
authors.initials: S
authors.lastname: Bian
authors.forename: Nuray
authors.initials: N
authors.lastname: Akyüz
authors.forename: Christian
authors.initials: C
authors.lastname: Bernreuther
authors.forename: Gabriele
authors.initials: G
authors.lastname: Loers
authors.forename: Ewa
authors.initials: E
authors.lastname: Laczynska
authors.forename: Igor
authors.initials: I
authors.lastname: Jakovcevski
authors.forename: Melitta
authors.initials: M
authors.lastname: Schachner
mesh_terms: D000818:Animals; D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000008:administration & dosage / Q000494:pharmacology; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D027341:Excitatory Amino Acid Transporter 1 / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D055495:Neurogenesis; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics / Q000378:metabolism; D055153:Stem Cell Niche; D015238:Sulfotransferases / Q000235:genetics / Q000378:metabolism*; D000097811:Carbohydrate Sulfotransferases
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D066246:ErbB Receptors; D027341:Excitatory Amino Acid Transporter 1; D051497:Receptor, Fibroblast Growth Factor, Type 2; D015238:Sulfotransferases; C499317:Ascl1 protein, mouse; C493393:Slc1a3 protein, mouse; C541485:EGFR protein, mouse; C496354:Fgfr2 protein, mouse; C557901:Chst11 protein, mouse; D000097811:Carbohydrate Sulfotransferases; C438013:dermatan-4-sulfotransferase-1