title: In vitro models of the blood-brain barrier.
abstract: The chapter provides an introduction and brief overview of currently available in vitro blood-brain barrier models, pointing out the major advantages and disadvantages of the respective models and potential applications. Bovine brain microvessel endothelial cell isolation, culture, and transendothelial permeability measurement procedures are discussed in detail as a model system for a laboratory to begin brain vascular investigations.
authors.affiliation: School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
authors.forename: Kaushik K
authors.initials: KK
authors.lastname: Shah
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Abbruscato
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001812:Blood-Brain Barrier / Q000166:cytology* / Q000502:physiology*; D001921:Brain / Q000098:blood supply* / Q000166:cytology; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology*; D010539:Permeability