title: Photochemical characterization of phytochrome missense mutants.
abstract: Phytochromes are photoreceptors that regulate many aspects of plant growth and development in response to red/far-red light signals from the environment. In this study, we analyzed chromophore ligation and photochromism of missense phytochrome mutants in the Per-Arnt-Sim (PAS)-related domain (PRD). Among the 14 mutants analyzed, the Gly768Asp mutant of Avena phytochrome A showed aberrant photochromism and dark reversion, suggesting that amino acid residues in the C-terminal domain affect the photochemical properties of the photosensory N-terminal domain.
authors.affiliation: Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju, Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Shin
authors.forename: Hwan-Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Si-Seok
authors.initials: SS
authors.lastname: Lee
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Han
authors.forename: Pill-Soon
authors.initials: PS
authors.lastname: Song
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Kim
mesh_terms: D017360:Arabidopsis; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D055668:Photochemical Processes; D010834:Phytochrome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D050505:Mutant Proteins; D010834:Phytochrome