title: Methallyl isothiocyanate inhibits the caspase-1 activity through the inhibition of intracellular calcium levels.
abstract: Isothiocyanates (ITCs) play a major role in the potential chemopreventive effect. Cruciferous vegetables are a particularly abundant source of ITCs. Methallyl ITC (MAITC) belongs to ITCs as a synthesized compound. However, the effects of MATIC have never been elucidated. The aim of this work was to investigate the anti-inflammatory effects and mechanisms of methallyl isothiocyanate (MAITC) in mast cells. MAITC suppressed the intracellular calcium levels in phorbol myristate acetate (PMA) plus calcium ionophore A23187 (PMACI)-stimulated human mast cell line (HMC-1) cells. MAITC significantly inhibited the production and mRNA expression of interleukin (IL)-1β in PMACI-stimulated HMC-1 cells. The activities of caspase-1 and receptor interacting protein-2 were significantly inhibited by the treatment with MAITC in PMACI-stimulated HMC-1 cells. The activation of nuclear factor-κB and phosphorylation of extracellular signal-regulated kinase and IκBα were inhibited by the treatment with MAITC. In addition, MAITC significantly inhibited the production and mRNA expression of IL-6 and tumor necrosis factor-α in PMACI-stimulated HMC-1 cells. Furthermore, MAITC significantly inhibited caspase-1 enzymatic activity and reactive oxygen species generation in PMA-stimulated HMC-1 cells. Taken together, we can conclude that MAITC showed an anti-inflammatory effect on mast cell-mediated inflammatory reaction.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Il-Kwang
authors.initials: IK
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D017879:Isothiocyanates / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D017879:Isothiocyanates; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C037152:isothiocyanic acid; D000001:Calcimycin; D020170:Caspase 1; D002118:Calcium