title: Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane.
abstract: The mitochondrial inner membrane harbors the complexes of the respiratory chain and translocase complexes for precursor proteins. We have identified a further subunit of the carrier translocase (TIM22 complex) that surprisingly is identical to subunit 3 of respiratory complex II, succinate dehydrogenase (Sdh3). The membrane-integral protein Sdh3 plays specific functions in electron transfer in complex II. We show by genetic and biochemical approaches that Sdh3 also plays specific functions in the TIM22 complex. Sdh3 forms a subcomplex with Tim18 and is involved in biogenesis and assembly of the membrane-integral subunits of the TIM22 complex. We conclude that the assembly of Sdh3 with different partner proteins, Sdh4 and Tim18, recruits it to two different mitochondrial membrane complexes with functions in bioenergetics and protein biogenesis, respectively.
authors.affiliation: Institut für Biochemie und Molekularbiologie, ZBMZ, Universität Freiburg, 79104 Freiburg, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Gebert
authors.forename: Michael
authors.initials: M
authors.lastname: Gebert
authors.forename: Silke
authors.initials: S
authors.lastname: Oeljeklaus
authors.forename: Karina
authors.initials: K
authors.lastname: von der Malsburg
authors.forename: David A
authors.initials: DA
authors.lastname: Stroud
authors.forename: Bogusz
authors.initials: B
authors.lastname: Kulawiak
authors.forename: Christophe
authors.initials: C
authors.lastname: Wirth
authors.forename: René P
authors.initials: RP
authors.lastname: Zahedi
authors.forename: Pavel
authors.initials: P
authors.lastname: Dolezal
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wiese
authors.forename: Oliver
authors.initials: O
authors.lastname: Simon
authors.forename: Agnes
authors.initials: A
authors.lastname: Schulze-Specking
authors.forename: Kaye N
authors.initials: KN
authors.lastname: Truscott
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
authors.forename: Peter
authors.initials: P
authors.lastname: Rehling
authors.forename: Bernard
authors.initials: B
authors.lastname: Guiard
authors.forename: Carola
authors.initials: C
authors.lastname: Hunte
authors.forename: Bettina
authors.initials: B
authors.lastname: Warscheid
authors.forename: Martin
authors.initials: M
authors.lastname: van der Laan
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Pfanner
authors.forename: Nils
authors.initials: N
authors.lastname: Wiedemann
mesh_terms: D004579:Electron Transport; D042963:Electron Transport Complex II / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000201:enzymology / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; C102549:TIM22 protein, S cerevisiae; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase