title: Matrix metalloproteinases (MMPs) activity in cultured canine bone marrow stromal cells (BMSCs).
abstract: Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition.
authors.affiliation: Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Tani
authors.forename: Masato
authors.initials: M
authors.lastname: Koga
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Oda
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Terai
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000201:enzymology*; D002478:Cells, Cultured; D004285:Dogs / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D017154:Stromal Cells / Q000201:enzymology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9