title: Simultaneous determination of thirty non-steroidal anti-inflammatory drug residues in swine muscle by ultra-high-performance liquid chromatography with tandem mass spectrometry.
abstract: An ultra-high-performance liquid chromatography with tandem mass spectrometric detection (UHPLC-MS/MS) method was established for the simultaneous determination of residues of thirty non-steroidal anti-inflammatory drugs (NSAIDs) in swine muscle. The samples were extracted with acetonitrile and phosphoric acid. The extracts were defatted with n-hexane, and then purified by HLB solid-phase extraction cartridge. Analysis was carried out on UHPLC-ESI-MS/MS working with multiple reaction monitoring mode with polarity switching. Limits of detection were between 0.4 μg/kg and 2.0 μg/kg, and limits of quantification were between 1.0 μg/kg and 5.0 μg/kg. The recoveries of NSAIDs were between 61.7% and 125.7% at spiked levels of 1.0-500 μg/kg. The repeatability was less than 8% and the within-laboratory reproducibility was not more than 12.3%. The method was reliable, convenient and sensitive.
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Hu
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Li
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Chen
authors.forename: Han-Hui
authors.initials: HH
authors.lastname: Dai
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Deng
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Yue
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Wang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Shen
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.forename: Shuang-Yang
authors.initials: SY
authors.lastname: Ding
authors.forename: Yue-Ning
authors.initials: YN
authors.lastname: Zhou
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Zhu
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Jiang
mesh_terms: D000097:Acetonitriles; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis; D057230:Limit of Detection; D016014:Linear Models; D009132:Muscles / Q000737:chemistry*; D010756:Phosphoric Acids; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010756:Phosphoric Acids; C030242:phosphoric acid; C032159:acetonitrile