title: [Investigation of 1q21 amplification in patients with multiple myeloma using I-FISH and cIg-FISH].
abstract: OBJECTIVE
  To investigate the prevlance of 1q21 amplification in patients with multiple myeloma (MM) and its correlation with the progression and prognosis of the disease.
  
  
  METHODS
  1q21 amplification was detected in 48 patients with MM using cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization analysis (cIg-FISH) and interphase fluorescence in situ hybridization (I-FISH) analysis combined with CD138 immunomagnetic cell sorting (MACS).
  
  
  RESULTS
  1q21 amplification (≥ 3 red signals) was detected in 26/48(54.2%) cases by cIg-FISH and 31/48 (64.6%) cases by I-FISH combined with CD138 MACS. There was a good consistency between the two methods (P>0.05). The mortality of patients with 1q21 amplification was significantly higher than those without (P< 0.05). No significant difference was detected in terms of sex, age, Durie-Salmon stage, subgroup and international staging system (ISS) stage between patients with 1q21 amplification and those without (P>0.05).
  
  
  CONCLUSIONS
  The frequency of 1q21 amplification in MM is high. There was also an association between the amplification and poor prognosis. cIg-FISH is consistent with CD138 MACS combined with I-FISH.
authors.affiliation: Tianjin Medical University Cancer Institute and Hospital, Tianjin Cancer Research Institute, Tianjin, People's Republic of China.
authors.forename: Rui-fang
authors.initials: RF
authors.lastname: Yang
authors.forename: Chun-ming
authors.initials: CM
authors.lastname: Li
authors.forename: Hai-rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Han-xin
authors.initials: HX
authors.lastname: Wu
authors.forename: Jia-ren
authors.initials: JR
authors.lastname: Xu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Jian-yong
authors.initials: JY
authors.lastname: Li
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D002878:Chromosomes, Human, Pair 1; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D011379:Prognosis; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D053668:Syndecan-1