title: Phages bearing affinity peptides to bovine rotavirus differentiate the virus from other viruses.
abstract: The aim of this study was to identify potential ligands and develop a novel diagnostic test to pathogenic bovine rotavirus (BRV) using phage display technology. The viruses were used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After five rounds of biopanning, phages had a specific binding activity to BRV were isolated. DNA sequencing indicated that phage displayed peptides HVHPPLRPHSDK, HATNHLPTPHNR or YPTHHAHTTPVR were potential ligands to BRV. Using the specific peptide-expressing phages, we developed a phage-based ELISA to differentiate BRV from other viruses. Compared with quantitative real-time PCR (qPCR), the phage-mediated ELISA was more suitable for the capture of BRV and the detection limitation of this approach was 0.1 µg/ml of samples. The high sensitivity, specificity and low cross-reactivity for the phage-based ELISA were confirmed in receiver operating characteristics (ROC) analysis.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
authors.forename: Yudong
authors.initials: Y
authors.lastname: Ren
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D019540:Area Under Curve; D001435:Bacteriophages / Q000378:metabolism; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000882:Haplorhini; D007668:Kidney / Q000166:cytology; D008024:Ligands; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods; D012372:ROC Curve; D012401:Rotavirus / Q000737:chemistry* / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
chemical_list: D008024:Ligands; D019151:Peptide Library; D010455:Peptides