title: Increased expression of TLR-2, COX-2, and SOD-2 genes in the peripheral blood leukocytes of opisthorchiasis patients induced by Opisthorchis viverrini antigen.
abstract: Re-infection with liver fluke, Opisthorchis viverrini, increases proinflammatory molecules involved in inflammation-mediated disease and carcinogenesis in an animal model. To clarify whether these genes respond to parasite antigen in peripheral blood leukocytes (PBL) of opisthorchiasis patients, we examined the transcriptional level of oxidant-generating (toll-like receptor 2 (TLR-2), nuclear factor-kappa B (NF-KB), and cyclooxygenase 2 (COX-2)), anti-oxidant-generating (manganese superoxide dismutase 2 (SOD-2) and catalase (CAT)), proinflammatory cytokine (interleukin (IL)-1β), and anti-inflammatory cytokine (IL-10), in PBL exposed to parasite antigen in O. viverrini-infected patients compared with healthy individuals in an in vitro experiment. After O. viverrini antigen-treated PBL, quantitative RT-PCR analysis revealed that increased expression of cytokines and oxidant-generating genes in PBL was similar between O. viverrini-infected and healthy groups. Interestingly, compared with healthy subjects, increase of TLR-2, COX-2, and SOD-2 and decreased CAT mRNA expression levels were observed in O. viverrini-infected group. The results indicate that O. viverrini antigen induces upregulation of TLR-2, COX-2, and SOD-2 and downregulation of CAT genes in opisthorchiasis patients, suggesting that imbalance of oxidant/anti-oxidant transcripts during re-infection may be involved in the inflammatory-driven carcinogenesis. These molecules may be used as the chemopreventive target for intervention of opisthorchiasis patients in an endemic area.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. puangrat@kku.ac.th
authors.forename: Puangrat
authors.initials: P
authors.lastname: Yongvanit
authors.forename: Raynoo
authors.initials: R
authors.lastname: Thanan
authors.forename: Somchai
authors.initials: S
authors.lastname: Pinlaor
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Watcharin
authors.initials: W
authors.lastname: Loilome
authors.forename: Nisana
authors.initials: N
authors.lastname: Namwat
authors.forename: Anchalee
authors.initials: A
authors.lastname: Techasen
authors.forename: Somkid
authors.initials: S
authors.lastname: Dechakhamphu
mesh_terms: D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006805:Human Experimentation; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000276:immunology / Q000473:pathology*; D009891:Opisthorchis / Q000276:immunology* / Q000472:pathogenicity; D060888:Real-Time Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis* / Q000276:immunology; D000099156:Superoxide Dismutase 2
chemical_list: D000947:Antigens, Helminth; D051546:Cyclooxygenase 2; D016207:Cytokines; D013482:Superoxide Dismutase; D051195:Toll-Like Receptor 2; D000099156:Superoxide Dismutase 2; C495342:TLR2 protein, human; C496540:PTGS2 protein, human