title: PrPC interacts with potassium channel tetramerization domain containing 1 (KCTD1) protein through the PrP(51-136) region containing octapeptide repeats.
abstract: To identify molecular interaction partners of the cellular prion protein (PrP(C)), we applied a yeast two-hybrid screen on a bovine brain cDNA expression library and identified the potassium channel tetramerization domain containing 1 (KCTD1) as a PrP(C) interacting protein. Deletion mapping showed that PrP(C) specifically binds KCTD1 through the unstructured PrP(51-136) region. We further confirmed the interaction between PrP(C) and KCDT1 protein by co-immunoprecipitation in vivo and by a biosensor assay in vitro. Interestingly, the binding of an insertion mutant PrP(8OR) to KCTD1 is higher than that of wild-type PrP(C), suggesting an important role for an unstructured region harboring octapeptide repeats in the KCTD1-PrP(C) interaction. Our results identify a novel PrP(C)-interacting protein and suggest a new approach to investigating the unidentified physiological cellular function of PrP(C).
authors.affiliation: The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.forename: Yajing
authors.initials: Y
authors.lastname: Lu
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Liqin
authors.initials: L
authors.lastname: Huang
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
authors.forename: Guihong
authors.initials: G
authors.lastname: Sun
mesh_terms: D000818:Animals; D002417:Cattle; D047468:Immunoprecipitation; D009842:Oligopeptides / Q000378:metabolism; D018621:PrPC Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D020449:Repetitive Sequences, Amino Acid; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D009842:Oligopeptides; D018621:PrPC Proteins; D012097:Repressor Proteins