title: Molecular techniques in ecohealth research toolkit: facilitating estimation of aggregate gastroenteritis burden in an irrigated periurban landscape.
abstract: Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques in a survey of Cryptosporidium parvum, Giardia lamblia, Campylobacter jejuni, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., Shigella spp., Vibrio cholera, and Rotavirus A densities in canal water with respect to seasonality and spatial distribution of point-nonpoint pollution sources. Three irrigational canals stretching across nearly a 150-km(2) periurban landscape, traditionally used for agricultural irrigation but function as vital part of municipal wastewater stabilization in recent years, were investigated. Compiled stochastic data (pathogen concentration, susceptible populations) and literature-obtained deterministic data (pathogen dose-response model parameter values) were used in estimating waterborne gastroenteritis burden. Exposure scenarios include swimming or fishing, consuming canal water-irrigated vegetables, and ingesting or inhaling water aerosols while working in canal water-irrigated fields. Estimated annual gastroenteritis burden due individual pathogens among the sampling points was -10.6log(10) to -2.2log(10) DALYs. Aggregated annual gastroenteritis burden due all the target pathogens per sampling point was -3.1log(10) to -1.9log(10) DALYs, far exceeding WHO acceptable limit of -6.0log(10) DALYs. The present approach will facilitate the comprehensive collection of surface water microbiological baseline data and setting of benchmarks for interventions aimed at reducing microbial hazards in similar landscapes worldwide.
authors.affiliation: WHO Collaborating Center for Water Supply and Waste Disposal, Environmental Engineering and Management, Asian Institute of Technology, P.O. Box 4, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Ariuntuya
authors.initials: A
authors.lastname: Tserendorj
authors.forename: Alfredo J
authors.initials: AJ
authors.lastname: Anceno
authors.forename: Eric R
authors.initials: ER
authors.lastname: Houpt
authors.forename: Crystal R
authors.initials: CR
authors.lastname: Icenhour
authors.forename: Orntipa
authors.initials: O
authors.lastname: Sethabutr
authors.forename: Carl S
authors.initials: CS
authors.lastname: Mason
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Shipin
mesh_terms: D057914:Agricultural Irrigation; D003141:Communicable Diseases; D017281:Cost of Illness; D017753:Ecosystem; D005759:Gastroenteritis / Q000175:diagnosis / Q000209:etiology* / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction; D012107:Research Design; D018570:Risk Assessment; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D013785:Thailand; D014505:Urban Population; D014871:Water Microbiology
chemical_list: D009841:Oligonucleotides