title: Identification and isolation of adult liver stem/progenitor cells.
abstract: Hepatoblasts are considered to be liver stem/progenitor cells in the fetus because they propagate and differentiate into two types of liver epithelial cells, hepatocytes and cholangiocytes. In adults, oval cells that emerge in severely injured liver are considered facultative hepatic stem/progenitor cells. However, the nature of oval cells has remained unclear for long time due to the lack of a method to isolate them. It has also been unclear whether liver stem/progenitor cells exist in normal adult liver. Recently, we and others have successfully identified oval cells and adult liver stem/progenitor cells. Here, we describe the identification and isolation of mouse liver stem/progenitor cells by utilizing antibodies against specific cell surface marker molecules.
authors.affiliation: Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan. tanaka@iam.u-tokyo.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyajima
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000503:physiopathology*; D000071858:Epithelial Cell Adhesion Molecule; D005434:Flow Cytometry / Q000379:methods*; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration / Q000502:physiology*; D051379:Mice; D011725:Pyridines / Q000633:toxicity; D013234:Stem Cells / Q000166:cytology*
chemical_list: C530773:3,5-diethoxycarbonyl-1,4-dihydrocollidine; D000906:Antibodies; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D011725:Pyridines; C544766:TROP2 protein, mouse