title: A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase.
abstract: A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB entry 1ee6), with a root-mean-square deviation of 0.93 Å and a sequence identity of 53%. This structural similarity is significant considering that C. bescii is a hyperthermophile and Bacillus sp. is a mesophile.
authors.affiliation: BioSciences Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Alahuhta
authors.forename: Puja
authors.initials: P
authors.lastname: Chandrayan
authors.forename: Irina
authors.initials: I
authors.lastname: Kataeva
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Michael E
authors.initials: ME
authors.lastname: Himmel
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Lunin
mesh_terms: D001406:Bacillaceae / Q000201:enzymology*; D055162:Biocatalysis; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D011133:Polysaccharide-Lyases; C023911:pectate lyase