title: Airway IFN-γ production during RSV bronchiolitis is associated with eosinophilic inflammation.
abstract: OBJECTIVE
  This study was designed to investigate the possible role of IFN-γ in eosinophil degranulation that occurs during respiratory syncytial virus (RSV) bronchiolitis.
  
  
  METHODS
  Sixty-seven infants, 2-24 months old and hospitalized with their first episode of acute RSV bronchiolitis, were selected for this study. Eosinophil-active cytokine and chemokine profiles in nasal lavage supernatants taken within the first 48 h of admission were determined by a multiplex bead array system (Luminex). Comparisons were made with control (Control group) subjects (n = 20).
  
  
  RESULTS
  Nasal IFN-γ levels were significantly higher (P < 0.0001) in RSV bronchiolitis (median = 4.4 pg/ml) infants compared to controls (0.0 pg/ml). IFN-γ levels correlated significantly with the levels of nasal eotaxin (r = 0.566, P < 0.0001), RANTES (r = 0.627, P < 0.0001), GM-CSF (r = 0.849, P < 0.0001), and EDN (r = 0.693, P < 0.001). Nasal interleukin (IL)-4, IL-5, and IL-13 were below sensitivity levels in most RSV bronchiolitis and control subjects.
  
  
  CONCLUSIONS
  These results suggest that IFN-γ may play an important role in eosinophilic inflammation in RSV bronchiolitis.
authors.affiliation: Department of Pediatrics, Asthma and Allergy Center, Inje University Sanggye Paik Hospital, Seoul, Korea.
authors.forename: Chang Keun
authors.initials: CK
authors.lastname: Kim
authors.forename: Zak
authors.initials: Z
authors.lastname: Callaway
authors.forename: Young Yull
authors.initials: YY
authors.lastname: Koh
authors.forename: Soo-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Takao
authors.initials: T
authors.lastname: Fujisawa
mesh_terms: D001988:Bronchiolitis / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D018946:Chemokine CCL5 / Q000378:metabolism; D002675:Child, Preschool; D047208:Eosinophil-Derived Neurotoxin / Q000378:metabolism; D004802:Eosinophilia / Q000150:complications / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000032:analysis / Q000378:metabolism*; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008297:Male; D017817:Nasal Lavage Fluid / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000378:metabolism*; D012136:Respiratory Syncytial Viruses; D018709:Statistics, Nonparametric
chemical_list: D018946:Chemokine CCL5; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D047208:Eosinophil-Derived Neurotoxin