title: Responses of three different ecotypes of reed (Phragmites communis Trin.) to their natural habitats: leaf surface micro-morphology, anatomy, chloroplast ultrastructure and physio-chemical characteristics.
abstract: The adaptational characteristics due to long-term adaptation in the natural habitats of common reed (Phragmites communis Trin.) contrasted considerably among three different ecotypes: dune reed (DR), Gobi salt reed (GSR) and swamp reed (SR). The micromorphologies of leaf adaxial surfaces showed tapered setae and a non-smooth surface in DR, compound papillose structures with wax and hairs in GSR, but only papillose structures for the smooth surface of SR. Anatomical analysis showed that DR and GSR had higher bundle-sheath cell areas and a lower xylem/phloem ratio than SR. There were many sclerenchyma cells in vascular bundle of DR and GSR and crystal idioblasts in all ecotypes. Chloroplasts had ellipsoid shape in SR, but they were attached to the cell wall with oblong shape and contained many starch grains in DR and GSR. Higher concentrations of NO, H(2)O(2) and lipid peroxidation, higher ratio of carotenoids/chlorophyll and higher activities in T-AOC and SOD were found in DR and GSR. Na(+)/K(+)-ATPase and Ca(2+)/Mg(2+)-ATPase activities were greatest in GSR. All these data suggested that the greater relative stress tolerance of DR and GSR was due to a combination of morpho-anatomical adaptational characteristics and physio-chemical responses, and indicated the different mechanisms in their respective natural habitats.
authors.affiliation: Extreme Stress Resistance and Biotechnology Laboratory, Shapotou Desert Research & Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou City, Gansu Province 730000, China. ybliu13@163.com
authors.forename: Yubing
authors.initials: Y
authors.lastname: Liu
authors.forename: Xinrong
authors.initials: X
authors.lastname: Li
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Cao
authors.forename: Huijuan
authors.initials: H
authors.lastname: Tan
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
mesh_terms: D000222:Adaptation, Physiological; D017301:Ca(2+) Mg(2+)-ATPase / Q000737:chemistry; D002129:Calcium Oxalate / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry / Q000648:ultrastructure*; D017753:Ecosystem; D060146:Ecotype; D004789:Enzyme Activation; D057075:Enzyme Assays; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation; D008854:Microscopy, Electron; D009569:Nitric Oxide / Q000737:chemistry; D052585:Phloem / Q000502:physiology; D018515:Plant Leaves / Q000502:physiology* / Q000648:ultrastructure; D006109:Poaceae / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D013045:Species Specificity; D052584:Xylem / Q000502:physiology
chemical_list: D012987:Soil; D002734:Chlorophyll; D002129:Calcium Oxalate; D009569:Nitric Oxide; D002338:Carotenoids; D006861:Hydrogen Peroxide; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase