title: "One-step" preparation of thiol-ene clickable PEG-based thermoresponsive hyperbranched copolymer for in situ crosslinking hybrid hydrogel.
abstract: A well-defined poly(ethylene glycol) based hyperbranched thermoresponsive copolymer with high content of acrylate vinyl groups was synthesized via a "one-pot and one-step" deactivation enhanced atom transfer radical polymerization approach, which provided an injectable and in situ crosslinkable system via Michael-type thiol-ene reaction with a thiol-modified hyaluronan biopolymer. The hyperbranched structure, molecular weight, and percentage of vinyl content of the copolymer were characterized by gel permeation chromatography and (1)H NMR. The lower critical solution temperature of this copolymer is close to body temperature, which can result in a rapid thermal gelation at 37 °C. The scanning electron microscopy analysis of crosslinked hydrogel showed the network formation with porous structure, and 3D cell culture study demonstrated the good cell viability after the cells were embedded inside the hydrogel. This injectable and in situ crosslinking hybrid hydrogel system offers great promise as a new class of hybrid biomaterials for tissue engineering.
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Yixiao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Aram Omer
authors.initials: AO
authors.lastname: Saeed
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Waqar
authors.initials: W
authors.lastname: Hassan
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Keigher
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Chemistry, Bangor University, Bangor, LL57 2UW, UK. h.tai@bangor.ac.uk.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Tai
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland.
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
authors.affiliation: Network of Excellence for Functional Biomaterials, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland. wenxin.wang@nuigalway.ie.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D057930:Click Chemistry / Q000379:methods*; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011092:Polyethylene Glycols; C000595209:polyethylene glycol 1000