title: Dielectric cell response in highly conductive buffers.
abstract: We present a novel method for the identification of live and dead T-cells, dynamically flowing within highly conductive buffers. This technique discriminates between live and dead (heat treated) cells on the basis of dielectric properties variations. The key advantage of this technique lies in its operational simplicity, since cells do not have to be resuspended in isotonic low conductivity media. Herein, we demonstrate that at 40 MHz, we are able to statistically distinguish between live and dead cell populations.
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington, London, SW7 2AZ, United Kingdom.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Gielen
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: deMello
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Edel
mesh_terms: D002021:Buffers; D002469:Cell Separation / Q000295:instrumentation*; D004553:Electric Conductivity; D006801:Humans; D019169:Jurkat Cells; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D002021:Buffers