title: Fibroblast growth factor 2-functionalized collagen matrices for skeletal muscle tissue engineering.
abstract: Fibroblast growth factor 2 (FGF2) protein plays important roles in wound healing and tissue regeneration. Collagen is clinically used for wound care applications. We investigated the potential value of FGF2-functionalized collagen matrices for skeletal muscle tissue engineering. When C2C12 cells were treated with FGF2, cell adhesion increased after 3 and 5 days compared to the control (P < 0.05). Wound healing activity of FGF2 was slightly higher than the control through cell migration. Cell proliferation activity of FGF2-functionalized collagen matrices on C2C12 cells also increased. Taken together, FGF2 stimulated C2C12 myoblast growth by promoting cell adhesion, proliferation and wound healing activity after injury. The potential effect of FGF2-functionalized collagen matrices was also observed. Thus FGF2 stimulates skeletal muscle development and regeneration, thereby leading to potential utility for skeletal muscle tissue engineering.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea.
authors.forename: Ye-Rang
authors.initials: YR
authors.lastname: Yun
authors.forename: Sujin
authors.initials: S
authors.lastname: Lee
authors.forename: Eunyi
authors.initials: E
authors.lastname: Jeon
authors.forename: Wonmo
authors.initials: W
authors.lastname: Kang
authors.forename: Kook-Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000818:Animals; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism*; D006133:Growth Substances / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects*; D032446:Myoblasts / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001688:Biological Products; D004337:Drug Carriers; D006133:Growth Substances; D016222:Fibroblast Growth Factor 2; D003094:Collagen