title: [Effects of fractalkine on the expression of inflammatory substances in LPS-activated microglia cells].
abstract: OBJECTIVE
  To investigate the effect of fractalkine on the expression of the tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and nitric oxide (NO) in LPS-activated murine microglia cells line N9.
  
  
  METHODS
  In vitro LPS-activated microglia cells were treated for 24 h in the presence of fractalkine. The level of TNF-α and IL-1β in the culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA), The level of NO in the culture supernatants were quantitated by the NO test assay.
  
  
  RESULTS
  The concentration of TNF-α, IL-1β and NO in the culture supernatants evidently increased in LPS-activated microglia cells groups and prominently decreased by the fractalkine co-incubated.
  
  
  CONCLUSIONS
  It is thus concluded that fractalkine has neuroprotective functions by inhibiting the expression of inflammatory factor in activated microglia cells.
authors.affiliation: Department of Laboratory, The Affiliated Union Hospital, Fujian Medical University, Fujian Medical University, Fuzhou 350004, China. zxj5027667@tom.com
authors.forename: Xian-jin
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Song
authors.forename: Qiu-yu
authors.initials: QY
authors.lastname: Zhang
authors.forename: Ying-ping
authors.initials: YP
authors.lastname: Cao
authors.forename: Wei-qun
authors.initials: WQ
authors.lastname: Xu
authors.forename: Dong-hui
authors.initials: DH
authors.lastname: Su
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D054428:Chemokine CX3CL1 / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D054428:Chemokine CX3CL1; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide