title: Synergistic induction of periodontal tissue regeneration by binary application of human osteogenic protein-1 and human transforming growth factor-β3 in Class II furcation defects of Papio ursinus.
abstract: OBJECTIVE
  Binary applications of recombinant human osteogenic protein-1 (hOP-1) and transforming growth factor-β3 (hTGF-β3) synergize to induce pronounced bone formation. To induce periodontal tissue regeneration, binary applications of hOP-1 and hTGF-β(3) were implanted in Class II furcation defects of the Chacma baboon, Papio ursinus.
  
  
  METHODS
  Defects were created bilaterally in the furcation of the first and second mandibular molars of three adult baboons. Single applications of 25 μg hOP-1 and 75 μg hTGF-β(3) in Matrigel(®) matrix were compared with 20:1 binary applications, i.e. 25 μg hOP-1 and 1.25 μg hTGF-β(3). Morcellated fragments of autogenous rectus abdominis striated muscle were added to binary applications. Sixty days after implantation, the animals were killed and the operated tissues harvested en bloc. Undecalcified sections were studied by light microscopy, and regenerated tissue was assessed by measuring volume and height of newly formed alveolar bone and cementum.
  
  
  RESULTS
  The hOP-1 and hTGF-β(3) induced periodontal tissue regeneration and cementogenesis. Qualitative morphological analysis of binary applications showed clear evidence for considerable periodontal tissue regeneration. Quantitatively, the differences in the histomorphometric values did not reach statistical significance for the group size chosen for this primate study. The addition of morcellated muscle fragments did not enhance tissue regeneration. Binary applications showed rapid expansion of the newly formed bone against the root surfaces following fibrovascular tissue induction in the centre of the treated defects.
  
  
  CONCLUSIONS
  Binary applications of hOP-1 and hTGF-β(3) in Matrigel(®) matrix in Class II furcation defects of P. ursinus induced substantial periodontal tissue regeneration, which was tempered, however, by the anatomy of the furcation defect model, which does not allow for the rapid growth and expansion of the synergistic induction of bone formation, particularly when additionally treated with responding myoblastic stem cells.
authors.affiliation: Bone Research Unit, Medical Research Council, University of Witwatersrand, Johannesburg, South Africa.
authors.forename: J A
authors.initials: JA
authors.lastname: Teare
authors.forename: J-C
authors.initials: JC
authors.lastname: Petit
authors.forename: U
authors.initials: U
authors.lastname: Ripamonti
mesh_terms: D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D025021:Cementogenesis / Q000187:drug effects; D003094:Collagen; D003739:Dental Cementum / Q000187:drug effects / Q000473:pathology; D004337:Drug Carriers; D004338:Drug Combinations; D004357:Drug Synergism; D017823:Furcation Defects / Q000145:classification / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D007797:Laminin; D008336:Mandibular Diseases / Q000601:surgery; D008963:Molar / Q000601:surgery; D010012:Osteogenesis / Q000187:drug effects; D048549:Papio ursinus; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D011509:Proteoglycans; D017568:Rectus Abdominis / Q000637:transplantation; D053782:Transforming Growth Factor beta3 / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: C525819:BMP7 protein, human; D001672:Biocompatible Materials; D055419:Bone Morphogenetic Protein 7; D004337:Drug Carriers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C509044:TGFB3 protein, human; D053782:Transforming Growth Factor beta3; C059485:matrigel; D003094:Collagen