title: Genetic polymorphism of MUC7 in individuals with aggressive or chronic periodontitis.
abstract: Individuals with periodontitis exhibit differential expression of mucin-glycoprotein-2 (MG2), a protein encoded by the MUC7 gene. It is well known that MG2 exerts bactericidal activity as well as exhibiting genetic polymorphism involving a variable number of tandem repeats (VNTR). In the present study, we assessed the distribution of allelic variants of the MUC7 gene in 22 individuals with aggressive periodonitis, 68 with chronic periodonitis, and 87 without periodonitis. Oral mucosal cells were collected, the DNA was extracted, and specific primers were used to amplify the region encoding the MUC7 tandem repeats (TRs). Polymerase chain reaction products were subjected to electrophoresis and analyzed on polyacrylamide gels stained with silver nitrate. Although the percentage distribution of homozygosity (6-6TR) and heterozygosity (5-6TR) showed variation among the groups, the observed differences were not statistically significant (P > 0.05; Fisher's Exact Test). The present results indicate that the expression of different numbers of TRs in this salivary mucin in the oral environment does not interfere with the etiopathogenesis of aggressive or chronic periodontitis.
authors.affiliation: Department of Dentistry, Pontiphical Catholic University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Giselle P
authors.initials: GP
authors.lastname: Gomes
authors.forename: Marina A L
authors.initials: MA
authors.lastname: Assis
authors.forename: Juliana S S
authors.initials: JS
authors.lastname: Fonseca
authors.forename: Paulo E A
authors.initials: PE
authors.lastname: de Souza
authors.forename: Elton G
authors.initials: EG
authors.lastname: Zenóbio
authors.forename: Dauro D
authors.initials: DD
authors.lastname: Oliveira
authors.forename: Rodrigo V
authors.initials: RV
authors.lastname: Soares
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D055113:Chronic Periodontitis / Q000235:genetics*; D003430:Cross-Sectional Studies; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D009061:Mouth Mucosa / Q000737:chemistry / Q000166:cytology; D009077:Mucins / Q000235:genetics*; D011110:Polymorphism, Genetic; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D055815:Young Adult
chemical_list: C083238:MUC7 protein, human; D009077:Mucins; D012471:Salivary Proteins and Peptides