title: Chemiluminescence response of murine macrophages on multilayer microfluidic chips.
abstract: We have demonstrated an integrated platform for microfluidics and chemiluminescence (CL) detection that is capable of parallel cell culture, convenient liquid manipulation, and sensitive chemiluminescent detection. Luminol-dependent CL responses induced by three different stimuli, phytohemagglutinin (PHA), concanavalin A (ConA), and lipopolysaccharides (LPS), which can evoke a CL response in macrophages, were evaluated on this microfluidic chip. We studied the dose-dependent effect of these three stimuli on CL response in murine macrophages. PHA produced the highest CL response compared to LPS and ConA. The CL intensity produced by PHA was 6.85 and four times higher than that by LPS and ConA, respectively, at the low concentration of 100 μg/ml. We have found microfluidic based CL to be a very useful screening tool, which is less laborious and more sensitive. This microfluidic system is disposable and capable of rapid device prototyping; it may prove to be very useful in clinical and pharmaceutical applications.
authors.affiliation: Research Center of Biosensors and Medical Instruments, Shenzhen Institutes of Advanced Technology, CAS, Shenzhen, China.
authors.forename: Linfen
authors.initials: L
authors.lastname: Yu
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gai
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D049408:Luminescent Agents; D010835:Phytohemagglutinins; D003208:Concanavalin A; D008165:Luminol