title: Mc-hES, a novel plasmid carrying human endostatin gene, inhibits nasopharyngeal carcinoma growth.
abstract: Conventional plasmids for gene therapy produce low-level and short-term gene expression. Here, we first created minicircle carrying endostatin (mc-hES) for measurement of transfection efficiency. Compared with pcDNA-hES, MC-mediated endostatin gene transfer in vitro resulted in seven-fold greater endostatin expression levels in transfected cells and inhibited the growth of Human umbilical vein endothelial cells (HUVEC) more efficiently. HUVEC cell migration and tube-formation assays suggested that MC-mediated endostatin gene has significant anti-migration and anti-tube-formation capacity than that in pcDNA-hES. In vivo experiments showed that after transfection, mc-hES inhibited the growth of nasopharyngeal carcinoma xenografts. The tumor inhibition rates of mc-hES and pcDNA-hES were 60.8% and 26.9%, respectively (P<0.05). MC-mediated intratumoral endostatin expression in vivo was 2.2-17.9 times higher than pcDNA-hES in xenografted mice and lasted for 20 days. Our results suggest that minicircle DNA vectors might be a promising vector for biotherapy and should be further investigated.
authors.affiliation: The Affiliated Tumor Hospital, Zhengzhou University, Zhengzhou, PRC.
authors.forename: B-L
authors.initials: BL
authors.lastname: Xu
authors.forename: L
authors.initials: L
authors.lastname: Yuan
authors.forename: J-X
authors.initials: JX
authors.lastname: Wu
authors.forename: N
authors.initials: N
authors.lastname: Xu
authors.forename: W-J
authors.initials: WJ
authors.lastname: Fang
authors.forename: P
authors.initials: P
authors.lastname: Zhao
authors.forename: W-L
authors.initials: WL
authors.lastname: Huang
mesh_terms: D000818:Animals; D002277:Carcinoma; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D004270:DNA, Circular / Q000008:administration & dosage* / Q000235:genetics; D043169:Endostatins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D056945:Hep G2 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D010957:Plasmids / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D004270:DNA, Circular; D043169:Endostatins