title: The effect of Rho kinase inhibitor Y-27632 on endotoxemia-induced intestinal apoptosis in infant rats.
abstract: The aim of this study was to evaluate the effect of Rho kinase inhibitor, Y-27632 on the intestinal apoptosis in endotoxemic infant rats. Wistar albino 15-17-day-old rat pups (n = 21) were randomized to three experimental groups: (1) controls; (2) endotoxemia (LPS); and (3) endotoxemia treated with Y-27632 (LPS + Y-27632). Endotoxemia was induced in rats by intraperitoneal (i.p) injection of lipopolysaccharide (Escherichia coli serotype 0111:B4; 10 mg/kg). Y-27632 was administered 5 mg/kg i.p at three times, just, 8 and 16 h after LPS injection. Twenty-four hours after LPS injection, intestinal apoptosis was assessed by hematoxylin and eosin staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and immunohistochemistry for active caspase-3. Endotoxemia induced extensive apoptotic injury in the intestinal tissues. The administration of Y-27632 to endotoxemic infant rats caused a marked decrease in the number of apoptotic cells in both intestinal epithelium and lamina propria. In conclusion, the inhibition of Rho kinase with Y-27632 diminished the intestinal apoptotic damage induced by endotoxemia in infant rats.
authors.affiliation: Department of Pediatrics, School of Medicine, Dokuz Eylul University, Izmir, Turkey. durgul.ozdemir@deu.edu.tr
authors.forename: Durgul
authors.initials: D
authors.lastname: Ozdemir
authors.forename: Serap
authors.initials: S
authors.lastname: Cilaker
authors.forename: Kazim
authors.initials: K
authors.lastname: Tugyan
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Dagdelen
authors.forename: Oksan
authors.initials: O
authors.lastname: Derinoz
authors.forename: Ensari
authors.initials: E
authors.lastname: Guneli
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D019446:Endotoxemia / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases; D053148:Caspase 3