title: Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits.
abstract: In vivo optogenetic strategies have redefined our ability to assay how neural circuits govern behavior. Although acutely implanted optical fibers have previously been used in such studies, long-term control over neuronal activity has been largely unachievable. Here we describe a method to construct implantable optical fibers to readily manipulate neural circuit elements with minimal tissue damage or change in light output over time (weeks to months). Implanted optical fibers readily interface with in vivo electrophysiological arrays or electrochemical detection electrodes. The procedure described here, from implant construction to the start of behavioral experimentation, can be completed in approximately 2-6 weeks. Successful use of implantable optical fibers will allow for long-term control of mammalian neural circuits in vivo, which is integral to the study of the neurobiology of behavior.
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, USA.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Sparta
authors.forename: Alice M
authors.initials: AM
authors.lastname: Stamatakis
authors.forename: Jana L
authors.initials: JL
authors.lastname: Phillips
authors.forename: Nanna
authors.initials: N
authors.lastname: Hovelsø
authors.forename: Ruud
authors.initials: R
authors.lastname: van Zessen
authors.forename: Garret D
authors.initials: GD
authors.lastname: Stuber
mesh_terms: D004594:Electrophysiology / Q000379:methods*; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods; D020007:Implants, Experimental; D009415:Nerve Net / Q000502:physiology*; D055100:Optical Fibers