title: Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater.
abstract: Olive oil mill wastewaters (OMWs) are characterised by low pH and a high content of mono- and polyaromatic compounds that exert microbial and phytotoxic activity. The laccase cDNA of the poxA1b gene from Pleurotus ostreatus, carrying a signal peptide sequence for enzyme secretion and driven by the CaMV 35S promoter, was cloned into a plant expression vector. Nuclear genetic transformation was carried out by co-cultivation of Agrobacterium tumefaciens with tobacco cv Samsun NN leaves and cells of five different microalgae accessions belonging to the genera Chlamydomonas, Chlorella and Ankistrodesmus. Transgenic plants and microalgae were able to express and secrete the recombinant laccase in the root exudates and the culture medium, respectively. In comparison to untransformed controls, the ability to reduce phenol content in OMW solution was enhanced up to 2.8-fold in transgenic tobacco lines and by up to about 40% in two microalgae accessions. The present work provides new evidence for metabolic improvement of green organisms through the transgenic approach to remediation.
authors.affiliation: Department of Soil, Plant, Environmental and Animal Production Sciences, School of Biotechnological Sciences, University of Naples Federico II, Portici, Italy. chiaiese@unina.it
authors.forename: Pasquale
authors.initials: P
authors.lastname: Chiaiese
authors.forename: Francesca
authors.initials: F
authors.lastname: Palomba
authors.forename: Filippo
authors.initials: F
authors.lastname: Tatino
authors.forename: Carmine
authors.initials: C
authors.lastname: Lanzillo
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pinto
authors.forename: Antonino
authors.initials: A
authors.lastname: Pollio
authors.forename: Edgardo
authors.initials: E
authors.lastname: Filippone
mesh_terms: D001673:Biodegradation, Environmental; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D005818:Genetic Engineering; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D058086:Microalgae / Q000201:enzymology / Q000235:genetics; D000069463:Olive Oil; D010636:Phenols / Q000378:metabolism*; D010938:Plant Oils / Q000302:isolation & purification; D030821:Plants, Genetically Modified; D020076:Pleurotus / Q000201:enzymology / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D014170:Transformation, Genetic; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D014874:Water Pollutants, Chemical; D042845:Laccase