title: Herbidospora mongoliensis sp. nov., isolated from soil, and reclassification of Herbidospora osyris and Streptosporangium claviforme as synonyms of Herbidospora cretacea.
abstract: A Gram-reaction-positive aerobic actinomycete, designated strain MN08-A0118(T), which produced short chains of non-motile spores on the tips of long sporophores and formed yellow-brown colonies with branched substrate mycelium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence analyses, strain MN08-A0118(T) was grouped into the genus Herbidospora, being most closely related to Streptosporangium claviforme (98.2%), Herbidospora osyris (98.2%), Herbidospora daliensis (98.2%), Herbidospora cretacea (97.9%) and Herbidospora yilanensis (97.4%). Chemotaxonomic data supported allocation of the strain to the genus Herbidospora. MK-10(H(4)) was the predominant menaquinone with minor amounts of MK-10(H(6)), MK-10(H(2)) and MK-9(H(4)); the fatty acid profile contained major amounts of iso-C(16:0), C(17:0) 10-methyl, iso-C(14:0) and iso-C(16:0) 2-OH; the phospholipid profile contained phosphatidylethanolamine, phosphatidylmethylethanolamine and glucosamine-containing phospholipids; and the whole-cell sugars included ribose, glucose, galactose, madurose and rhamnose (trace). The phylogenetic data, phenotypic and genotypic properties and DNA-DNA hybridization differentiated this strain from its closely related strains, S. claviforme (35-54% DNA-DNA relatedness), H. osyris (39-51%), H. daliensis (3-16%), H. cretacea (34-39%) and H. yilanensis (34-42%). Thus, MN08-A0118(T) represents a novel species of the genus Herbidospora, for which the name Herbidospora mongoliensis sp. nov. is proposed, with MN08-A0118(T) ( = NBRC 105882(T)  = VTCC D9-22(T)) as the type strain. In addition, DNA-DNA hybridization results showed that S. claviforme and H. osyris are synonyms of H. cretacea.
authors.affiliation: Biotechnology Development Center, Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan.|Department of Botany and Microbiology, College of Science, King Saud University, Post Box 22452, Riyadh 11495, Kingdom of Saudi Arabia.
authors.forename: Ismet
authors.initials: I
authors.lastname: Ara
authors.affiliation: Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia.
authors.forename: Baljinova
authors.initials: B
authors.lastname: Tsetseg
authors.affiliation: Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia.
authors.forename: Damdinsuren
authors.initials: D
authors.lastname: Daram
authors.affiliation: Biotechnology Development Center, Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Suto
authors.affiliation: Biotechnology Development Center, Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ando
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S