title: Molecular identification of extended-spectrum-β-lactamase genes from Enterobacteriaceae isolated from healthy human carriers in Switzerland.
abstract: In this study, fecal samples from 586 healthy humans were investigated to determine the occurrence of extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae in Swiss people. A total of 5.8% of the human fecal samples yielded ESBL producers, and all of the 34 isolated strains were Escherichia coli. PCR analysis revealed that 14 strains produced CTX-M-15, 10 produced CTX-M-1, 7 strains produced CTX-M-14, and 2 strains produced CTX-M-2 ESBLs. One strain produced SHV-12 ESBL. Of the 34 isolates, 15 produced additional TEM-1 broad-spectrum β-lactamases. By serotyping, a high degree of diversity among the strains was found.
authors.affiliation: Vetsuisse Faculty, Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland.
authors.forename: Nadine
authors.initials: N
authors.lastname: Geser
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
authors.forename: Bozena M
authors.initials: BM
authors.lastname: Korczak
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Herbert
authors.initials: H
authors.lastname: Hächler
mesh_terms: D000328:Adult; D002353:Carrier State; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013557:Switzerland; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C071167:beta-lactamase CTX-2; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1