title: High-resolution full-field optical coherence microscopy using a Mirau interferometer for the quantitative imaging of biological cells.
abstract: In this paper quantitative imaging of biological cells using high-resolution full-field optical coherence microscopy (FF-OCM) is reported. The FF-OCM was realized using a swept-source system, a Mirau interferometer, and a CCD camera (a two-dimensional detection unit). A Mirau-interferometric objective lens was used to generate the interferometric signal. The signal was analyzed by a Fourier analysis technique. Optically sectioned amplitude images and a quantitative phase map of biological cells such as onion skin and red blood cells (RBCs) are demonstrated. Further, the refractive index profile of the RBCs is also presented. For the 50× Mirau objective, the experimentally achieved axial and transverse resolution of the present system are 3.8 and 1.2 μm, respectively. The CCD provides parallel detection and measures enface images without X, Y, Z mechanical scanning.
authors.affiliation: Laser Applications and Holography Laboratory, Instrument Design Development Centre, Indian Institute of Technology Delhi, Hauz Khas, New Delhi-110016, India.
authors.forename: Tulsi
authors.initials: T
authors.lastname: Anna
authors.forename: Vishal
authors.initials: V
authors.lastname: Srivastava
authors.forename: Dalip Singh
authors.initials: DS
authors.lastname: Mehta
authors.forename: Chandra
authors.initials: C
authors.lastname: Shakher
mesh_terms: D000465:Algorithms; D002477:Cells / Q000648:ultrastructure*; D004867:Equipment Design; D004912:Erythrocytes / Q000648:ultrastructure; D005583:Fourier Analysis; D021621:Imaging, Three-Dimensional; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D019697:Onions / Q000648:ultrastructure; D012031:Refractometry