title: Polymorphic microsatellite  loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and  cross-amplification in other congeneric species.
abstract: Lycoris  radiata is a perennial herb that has been used in traditional  Chinese medicine for a long time and has two main medicinal components in its  bulb, lycorine and galanthamine. However, the original microsatellite loci have  not been developed for any species of Lycoris. Total genomic DNA was  extracted from fresh bulbs using a modified CTAB protocol. We isolated 10  microsatellite loci from 21 L. radiata individuals of a natural population  from Yellow Mountain in Anhui Province, China. The number of alleles ranged  from two to nine. The observed and expected heterozygosities ranged from 0.238  to 0.952 and from 0.455 to 0.784, respectively. One locus significantly  deviated from Hardy-Weinberg equilibrium and no significant linkage  disequilibrium was found between pairs of loci. Cross-species amplification of  these microsatellite loci was characterized in additional five species (L.  sprengeri, L. anhuiensis, L. albiflora, L. longituba,  and L. chinensis) of Lycoris. The results suggest that these  microsatellite markers would contribute to the population genetic studies of L.  radiata and other related species.
authors.affiliation: Key Laboratory of Molecular Evolution and Biodiversity, Institute of Molecular Biology and Biotechnology, Anhui Normal University, Wuhu, PR China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Xuan
authors.forename: J Y
authors.initials: JY
authors.lastname: Zheng
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: P
authors.initials: P
authors.lastname: Gao
authors.forename: S B
authors.initials: SB
authors.lastname: Zhou
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: G-P
authors.initials: GP
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D002681:China; D003001:Cloning, Molecular; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D004926:Escherichia coli; D005787:Gene Frequency; D008040:Genetic Linkage; D056426:Genetic Loci; D005828:Genetics, Population; D018745:Genome, Plant; D015698:Genomic Library; D006579:Heterozygote; D031426:Lycoris / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D018517:Plant Roots / Q000235:genetics*; D011110:Polymorphism, Genetic; D014169:Transformation, Bacterial
chemical_list: D002593:Cetrimonium Compounds; D017931:DNA Primers; D018744:DNA, Plant; D000077286:Cetrimonium