title: Analysis of chromatin structure in plant cells.
abstract: A vast body of evidence in the literature indicates that nucleosomes can act as barriers to transcriptional initiation. The nucleosome at the promoter inhibits association of transcription factors disallowing active transcription of the gene. We have found a nucleosome on tobacco pathogenesis-related gene-1a (PR-1a) core promoter and mapped its boundaries and extension to find its span. The nucleosome covers the TATA box and Inr region of the core promoter and gets disassembled upon induction. Prior to its removal, modifications (i.e., acetylation and methylation of histones) occur at the nucleosome, proving a role of epigenetic modifications in transcriptional regulation. We summarize here various methodologies to analyze promoter chromatin structure in plants using the PR-1a core promoter as an example.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, UP, India.
authors.forename: Mala
authors.initials: M
authors.lastname: Singh
authors.forename: Amol
authors.initials: A
authors.lastname: Ranjan
authors.forename: Krishan Mohan
authors.initials: KM
authors.lastname: Rai
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Verandra
authors.initials: V
authors.lastname: Kumar
authors.forename: Ila
authors.initials: I
authors.lastname: Trivedi
authors.forename: Niraj
authors.initials: N
authors.lastname: Lodhi
authors.forename: Samir V
authors.initials: SV
authors.lastname: Sawant
mesh_terms: D000906:Antibodies / Q000276:immunology; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence; D002843:Chromatin / Q000737:chemistry*; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D059828:Plant Cells / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D013997:Time Factors
chemical_list: D000906:Antibodies; D002843:Chromatin; D017931:DNA Primers; D018744:DNA, Plant; D006657:Histones; D009707:Nucleosomes; D008836:Micrococcal Nuclease