title: Performance of V3-based HIV-1 sero subtyping in HIV endemic areas.
abstract: HIV-1 serosubtyping based on reactivity to peptides from the V3 region of gp120 is a low-cost and easy to perform procedure often used in geographical areas with high prevalence and incidence of HIV infection. We evaluated the performance of V3-based serotyping on 148 sera from 118 HIV-1-infected individuals living in Uganda, with estimated dates of seroconversion. Of the 148 tested samples, 68 (46.0%) specifically reacted with only one of the V3 peptides included in the test (SP), 64 (43.2%) did not react with any peptide (NR) and 16 (10.8%) reacted with two or more peptides (CR). According to the estimated seroconversion date, the large majority of samples collected early after infection belonged to the NR group. These samples had also a low Avidity Index. In contrast, samples collected later after infection belonged mainly to CR and SP groups and had also a higher avidity index. These results indicate that the performance of V3-based assays depends on maturation of HIV-specific immune response and can be significantly lowered when these tests are carried out on specimens collected from recently infected individuals.
authors.affiliation: Centro Nazionale AIDS, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Tavoschi
authors.forename: Daniela
authors.initials: D
authors.lastname: Bernasconi
authors.forename: Michele
authors.initials: M
authors.lastname: Chiappi
authors.forename: Barbara
authors.initials: B
authors.lastname: Suligoi
authors.forename: Claudio
authors.initials: C
authors.lastname: Galli
authors.forename: Vincenza
authors.initials: V
authors.lastname: Regine
authors.forename: Cissy
authors.initials: C
authors.lastname: Kityo
authors.forename: Stefano
authors.initials: S
authors.lastname: Buttò
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000032:analysis; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D019353:Endemic Diseases; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology; D006679:HIV Seropositivity; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D012703:Serotyping / Q000379:methods*; D014454:Uganda; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments