title: The fibrillogenic L178H variant of apolipoprotein A-I forms helical fibrils.
abstract: A number of amyloidogenic variants of apoA-I have been discovered but most have not been analyzed. Previously, we showed that the G26R mutation of apoA-I leads to increased β-strand structure, increased N-terminal protease susceptibility, and increased fibril formation after several days of incubation. In vivo, this and other variants mutated in the N-terminal domain (residues 26 to ∼90) lead to renal and hepatic accumulation. In contrast, several mutations identified within residues 170 to 178 lead to cardiac, laryngeal, and cutaneous protein deposition. Here, we describe the structural changes in the fibrillogenic variant L178H. Like G26R, the initial structure of the protein exhibits altered tertiary conformation relative to wild-type protein along with decreased stability and an altered lipid binding profile. However, in contrast to G26R, L178H undergoes an increase in helical structure upon incubation at 37°C with a half time (t(1/2)) of about 12 days. Upon prolonged incubation, the L178H mutant forms fibrils of a diameter of 10 nm that ranges in length from 30 to 120 nm. These results show that apoA-I, known for its dynamic properties, has the ability to form multiple fibrillar conformations, which may play a role in the tissue-specific deposition of the individual variants.
authors.affiliation: Department of Experimental Medical Sciences, Lund University, S-221 84 Lund, Sweden; and.
authors.forename: Jitka
authors.initials: J
authors.lastname: Petrlova
authors.affiliation: Department of Chemistry, California State University Sacramento, Sacramento, CA 95819.
authors.forename: Trang
authors.initials: T
authors.lastname: Duong
authors.affiliation: Department of Chemistry, California State University Sacramento, Sacramento, CA 95819.
authors.forename: Megan C
authors.initials: MC
authors.lastname: Cochran
authors.affiliation: Department of Experimental Medical Sciences, Lund University, S-221 84 Lund, Sweden; and.
authors.forename: Annika
authors.initials: A
authors.lastname: Axelsson
authors.affiliation: Department of Experimental Medical Sciences, and Department of Infection Medicine, Lund University, S-221 84 Lund, Sweden; and.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.affiliation: Department of Chemistry, California State University Sacramento, Sacramento, CA 95819. Electronic address: jens.lagerstedt@med.lu.se.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Roberts
authors.affiliation: Department of Experimental Medical Sciences, Lund University, S-221 84 Lund, Sweden; and. Electronic address: robertslm@csus.edu.
authors.forename: Jens O
authors.initials: JO
authors.lastname: Lagerstedt
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008854:Microscopy, Electron; D009154:Mutation; D055550:Protein Stability; D017433:Protein Structure, Secondary
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL