title: Fetal exposure to bisphenol A affects the primordial follicle formation by inhibiting the meiotic progression of oocytes.
abstract: Bisphenol A (BPA) is an estrogenic environmental toxin widely used for the production of plastics. Frequent human exposure to this chemical has been proposed to be a potential public health risk. The objective of this study was to assess the effects of BPA on germ cell cyst breakdown and primordial follicle formation. Pregnant mice were treated with BPA at doses of 0, 0.02, 0.04, 0.08 mg/kg body weight/day from 12.5 day postcoitum. BPA was delivered orally to pregnant female mice. A dose-response relationship was observed with increased BPA exposure level associated with more oocytes in germ cell cyst and less primordial follicle at postnatal day 3 (P < 0.01). Progression to meiosis prophase I of oocytes was delayed in the 0.08 mg/kg bw/day treated group (P < 0.01). Decreased mRNA expression of specific meiotic genes including Stra8, Dmc1, Rec8 and Scp3 were observed. In conclusion, BPA exposure can affect the formation of primordial follicle by inhibiting meiotic progression of oocytes.
authors.affiliation: Laboratory of Germ Cell Biology, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Han-Qiong
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Xi-Feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Hu-He
authors.initials: HH
authors.lastname: Chao
authors.forename: Bo
authors.initials: B
authors.lastname: Pan
authors.forename: Yan-Min
authors.initials: YM
authors.lastname: Feng
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001483:Base Sequence; D001559:Benzhydryl Compounds; D019175:DNA Methylation / Q000187:drug effects / Q000235:genetics; D005260:Female; D005333:Fetus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D018811:Maternal Exposure; D008540:Meiosis / Q000187:drug effects*; D049469:Meiotic Prophase I / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001559:Benzhydryl Compounds; D010636:Phenols; D011506:Proteins; D012333:RNA, Messenger; C102019:Stra8 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds