title: Lensless multispectral digital in-line holographic microscope.
abstract: An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.
authors.affiliation: University College Dublin, Communications and Optoelectronic Research Centre, Belfield, Dublin D4, Ireland.
authors.forename: James P
authors.initials: JP
authors.lastname: Ryle
authors.forename: Susan
authors.initials: S
authors.lastname: McDonnell
authors.forename: John T
authors.initials: JT
authors.lastname: Sheridan
mesh_terms: D000465:Algorithms; D000818:Animals; D002460:Cell Line; D003116:Color; D006224:Cricetinae; D004867:Equipment Design; D006696:Holography / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D008962:Models, Theoretical; D055100:Optical Fibers; D012815:Signal Processing, Computer-Assisted