title: Development and characterization of polymorphic microsatellite markers for Conopholis americana (Orobanchaceae).
abstract: OBJECTIVE
  Conopholis americana is an obligate root parasite with highly reduced morphology. To investigate the population structure, genetic diversity, and mating system of this predominantly eastern North American species, we developed polymorphic microsatellite markers for C. americana.
  
  
  RESULTS
  Using an enrichment cloning protocol, we report the isolation and characterization of 11 microsatellite markers. Product size varied from 198-370 bp. These loci show moderate levels of allelic variation (averaging 4.182 alleles per locus) and very low levels of heterozygosity (average observed heterozygosity = 0.054).
  
  
  CONCLUSIONS
  These microsatellite markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. americana.
authors.affiliation: Department of Biology, University of Toronto Mississauga, Ontario, Canada. anuar.rodrigues@utoronto.ca
authors.forename: Anuar G
authors.initials: AG
authors.lastname: Rodrigues
authors.forename: Alison E L
authors.initials: AE
authors.lastname: Colwell
authors.forename: Sasa
authors.initials: S
authors.lastname: Stefanovic
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D031670:Orobanchaceae / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers