title: Assessing wheat (Triticum  aestivum) genotypes for "Yr" resistance genes using conserved regions and simple-sequence motifs.
abstract: Analysis  of DNA sequence variation among genotypes is useful for differentiation of  wheat accessions, selection strategies and genetic development of crop plants.  We screened molecular markers for yellow rust resistance genes (Yr7, Yr9, Yr15, Yr18, Yr26, and YrH52), which are in the  gene-rich regions of wheat chromosomes 1B, 2B, and 7D, to investigate DNA  sequence differences and repeat motifs and numbers between wheat cultivars  resistant (Izgi2001, Sonmez2001, PI178383) and susceptible (Aytin98, ES14,  Harmankaya99) to yellow rust. The F(2) individuals  derived from the crosses were evaluated for yellow rust resistance at both the  seedling and adult stages to identify DNA markers genetically linked to yellow  rust resistance. The most repeated motif was found to be GA and the least  repeated motif TAGA among the cultivars. When we examined DNA sequence  differences (insertion, deletion and single nucleotide changes), the molecular  markers Xgwm526 (Yr7) and Xgwm273 (YrH52) were  found to have the most conserved regions and Yr15 (Xgwm413) the  least conserved regions among the cultivars. This DNA sequence information can  be used for selection of suitable parents, creating mapping populations and  developing molecular markers associated with yellow rust resistance in plant  breeding programs.
authors.affiliation: Department of Biology, Faculty of Science and Letters, Marmara University, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Cabuk
authors.forename: Y
authors.initials: Y
authors.lastname: Aydin
authors.forename: A A
authors.initials: AA
authors.lastname: Uncuoglu
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000502:physiology; D017124:Conserved Sequence / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D059372:Nucleotide Motifs / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers