title: Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells.
abstract: A femtosecond laser based transfection method using off-resonance plasmonic gold nanoparticles is described. For human cancer melanoma cells, the treatment leads to a very high perforation rate of 70%, transfection efficiency three times higher than for conventional lipofection, and very low toxicity (<1%). Off-resonance laser excitation inhibited the fracture of the nanoparticles into possibly toxic DNA intercalating particles. This efficient and low toxicity method is a promising alternative to viral transfection for skin cancer treatment.
authors.affiliation: École Polytechnique de Montréal, Laser Processing and Plasmonics Laboratory, Engineering Physics Department, Montréal, Québec H3C 3A7, Canada.
authors.forename: Judith
authors.initials: J
authors.lastname: Baumgart
authors.forename: Laure
authors.initials: L
authors.lastname: Humbert
authors.forename: Étienne
authors.initials: É
authors.lastname: Boulais
authors.forename: Rémi
authors.initials: R
authors.lastname: Lachaine
authors.forename: Jean-Jaques
authors.initials: JJ
authors.lastname: Lebrun
authors.forename: Michel
authors.initials: M
authors.lastname: Meunier
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007834:Lasers; D008545:Melanoma / Q000235:genetics / Q000628:therapy; D053758:Nanoparticles / Q000737:chemistry; D012878:Skin Neoplasms / Q000235:genetics* / Q000628:therapy*; D014162:Transfection / Q000379:methods*
chemical_list: D006046:Gold