title: [Use of fluorescent in situ hybridization in the cytogenetic diagnosis of urinary bladder urothelial carcinoma].
abstract: Our study was concerned with assessing the results of FISH analysis as a procedure to detect occult features of tumor and contribute to individualizing treatment strategies. It included a cohort of 50 patients with primary bladder tumors who had undergone transurethral resection. Washings from all patients were tested using UroVysion protocol immediately prior to surgery. Patients were divided into groups according to stage and grade of tumor. The sensitivities were 81.5%, 91.7% and 100% for Ta, T1 and T2 groups, respectively. In G1, G2 and G3 groups, the sensitivities were 70%, 100% and 100%, respectively. Besides, the rate of detecting genetically abnormal cells was significantly higher at stage T2 as compared with Ta and Ta+T1 groups. It was also higher in G3 group as compared with G1 for Ta and G2. No significant differences in the mean number of signals from each chromosome was reported. Frequency rates of abnormal cells of less than 40% correlated with absence of invasion into the muscular layer and poorly-differentiated cell status. They rose insignificantly in proportion to stage and grade. Detection of less than 40% of genetically abnormal cells correlated with absence of mascular invasion and G3 tumor (90% credibility). FISH diagnostic procedure showed high sensitivity of detecting even at incipient stage of bladder carcinoma and was instrumental in preoperative predicting type of tumor.
authors.forename: K A
authors.initials: KA
authors.lastname: Malkhasian
authors.forename: S V
authors.initials: SV
authors.lastname: Petrov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Ul'ianin
authors.forename: E F
authors.initials: EF
authors.lastname: Abdrakhmanov
authors.forename: R Sh
authors.initials: RSh
authors.lastname: Khasanov
mesh_terms: D000328:Adult; D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009154:Mutation; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D013520:Urologic Surgical Procedures