title: Molecular characterization of a Ran isoform gene up-regulated in shrimp immunity.
abstract: Diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. Ran gene was an important antiviral gene identified from shrimp and its mRNA level was up-regulated in response to viral infection. In this investigation, a Ran isoform gene (named Ran-iso) cDNA was cloned from shrimp, Marsupenaeus japonicus. The full-length cDNA of Ran-iso was 1286 bp, including a 5'-terminal untranslated region (UTR) of 272 bp, 3'-terminal UTR of 366 bp and an open reading frame (ORF) of 648 bp encoding a polypeptide of 215 amino acids. The deduced protein was highly homologous, it shared 90.64%, 84.19%, 81.48% and 67.58% identities with Ran protein of shrimp, honey bee, human and tobacco respectively. Ran-iso gene was constitutively expressed in 6 tissues examined, including gill, hepatopancreas, hemolymph, heart, intestine and muscle. However, Ran-iso was highest expressed in hepatopancreas (p<0.01), whereas the expressions of other five tissues were equal and relatively low. Time course analysis showed that the expression level of Ran-iso was obviously up-regulated 2.8 times (at 6h) as much as that in the control in the hepatopancreas challenged by WSSV. This investigation might provide a clue to elucidate the shrimp innate immunity and would be helpful to shrimp disease control.
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D001516:Bees / Q000235:genetics; D005786:Gene Expression Regulation; D043143:Hepatopancreas / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020033:Protein Isoforms / Q000235:genetics; D017385:Sequence Homology; D014026:Nicotiana / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D046848:White spot syndrome virus 1 / Q000276:immunology; D020931:ran GTP-Binding Protein / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D020931:ran GTP-Binding Protein