title: In vitro and in vivo evaluation of camptothecin nanosuspension: a novel formulation with high antitumor efficacy and low toxicity.
abstract: The purpose of this study was to evaluate the in vitro and in vivo antitumor efficacy and the dose dependent toxicity of camptothecin nanosuspension (Nano-CPT) comparing with that of topotecan (TPT). A novel supercritical antisolvent (SAS) process-high pressure homogenization technique has been developed to prepare Nano-CPT. The cytotoxicity of Nano-CPT and TPT was investigated against MCF-7, HCT-8, and PC-3 cell lines using MTT assay, antitumor activity in vivo were evaluated against HCT-8 xenograft model, and the dose dependent toxicity in vivo during the treatment were investigated by body weight changes and relative organ weight variations. The Nano-CPT presents about 6 times in vitro cytotoxicity active than TPT against cell lines MCF-7, nearly the same in vivo antitumor activity with TPT and lower toxicity. The results confirm that Nano-CPT is a novel potential formulation with high antitumor efficacy and low toxicity.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yao
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhao
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Li
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Fu
authors.forename: Baishi
authors.initials: B
authors.lastname: Zu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Meng
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001835:Body Weight / Q000187:drug effects; D002166:Camptothecin / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical; D025924:Chromatography, Supercritical Fluid; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D059004:Topoisomerase I Inhibitors / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D019772:Topotecan / Q000494:pharmacology; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D059004:Topoisomerase I Inhibitors; D019772:Topotecan; D002166:Camptothecin