title: Thalassospira permensis sp. nov., a new terrestrial halotolerant bacterium isolated from a naphthalene-utilizing microbial consortium.
abstract: A halotolerant bacterium, strain SMB34T, was isolated from a naphthalene-utilizing bacterial consortium obtained from primitive technogeneous soil (Vrkhnekamsk salt deposit, Perm region, Russia) by enrichment procedure. The strain itself was unable to degrade naphthalene and grew at NaCl concentrations up to 11% (w/v). The 16S rRNA-based phylogenetic analysis showed that the strain belongs to the genus Thalassospira. The DNA-DNA hybridization values between SMB34T and the type strains of phylogenetically closest species (T. xiamenensis, T. profundimaris and T. tepidiphila) did not exceed 50%. The novel strain could be distinguished from the above species by the cell motility, MALDI/TOF mass spectra of whole cells and a range of physiological and biochemical characteristics. SMB34T also considerably differs from the recently described species T. xianhensis, with the most striking differences in the DNA G + C content (53.7 +/- 1.0 vs. 61.2 +/- 1.0 mol.%) and predominant ubiquinones (Q-10 vs. Q-9). The data obtained suggest strain SMB34T (=VKM B-2527T = NBRC 106175T), designated as the type strain, represents a novel species, named Thalassospira permensis sp. nov.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Russian Academy of Sciences, Ural Branch, 13, Goleva str., Perm, 614081, Russia. peg@iegm.ru
authors.forename: E G
authors.initials: EG
authors.lastname: Plotnikova
authors.forename: L N
authors.initials: LN
authors.lastname: Anan'ina
authors.forename: V I
authors.initials: VI
authors.lastname: Krausova
authors.forename: E V
authors.initials: EV
authors.lastname: Ariskina
authors.forename: N V
authors.initials: NV
authors.lastname: Prisyazhnaya
authors.forename: A T
authors.initials: AT
authors.lastname: Lebedev
authors.forename: V A
authors.initials: VA
authors.lastname: Demakov
authors.forename: L I
authors.initials: LI
authors.lastname: Evtushenko
mesh_terms: D001482:Base Composition; D059013:Microbial Consortia; D009281:Naphthalenes / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D012426:Russia; D012988:Soil Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009281:Naphthalenes; D012336:RNA, Ribosomal, 16S; C031721:naphthalene