title: F-actin staining of Drosophila testes.
abstract: Preparations of Drosophila testes fixed with paraformaldehyde can be stained for F-actin according to the protocol described here. This staining procedure is particularly suitable for staining the male fusome and the cytokinetic contractile ring.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bonaccorsi
authors.forename: Maria G
authors.initials: MG
authors.lastname: Giansanti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenci
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D004330:Drosophila / Q000033:anatomy & histology*; D005404:Fixatives / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism; D008297:Male; D011108:Polymers / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D013737:Testis / Q000033:anatomy & histology / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods
chemical_list: D000199:Actins; D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; C003043:paraform