title: Identification of antigenic variants of the porcine rubulavirus in sera of field swine and their seroprevalence.
abstract: We sampled sera from 1013 non-vaccinated swine from four states in Mexico, Guanajuato, Jalisco, Michoacán and the Estado de Mexico, to analyse anti-porcine rubulavirus antibody titres against three different porcine rubulavirus isolates (PAC-4/1993, PAC-6/2001, and PAC-9/2003) using a hemagglutination inhibition assay. The results revealed that there were antigenic differences among the isolates assessed. In particular, the estimated correlation between the PAC-4/1993 and PAC-6/2001 (0.50) isolates and between the PAC-4/1993 and PAC-9/2003 isolates (0.56) displayed a moderate positive correlation. In contrast, there was a strong positive correlation between the PAC-6/2001 and PAC-9/2003 isolates (0.73). We also found that in the state of Guanajuato, PAC-4/1993 was the isolate that was most frequently identified; in Jalisco, the isolate was PAC-6/2001; and in Michoacán, the isolate was PAC-9/2003. By contrast, in the Estado de Mexico, all three isolates appeared to circulate with a low seroprevalence. In general, the analysed sera from the four states displayed a porcine rubulavirus serological prevalence ranging from 9% to 23.7%. These data indicate that there is not complete antibody cross-antigenicity among the three isolates, and the antigenic variations in the antibody response found in this study implies that the use of a monovalent vaccine would not generate complete protection against the different antigenic subtypes.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Distrito Federal, CP, México.
authors.forename: A C
authors.initials: AC
authors.lastname: Escobar-López
authors.forename: J F
authors.initials: JF
authors.lastname: Rivera-Benitez
authors.forename: H
authors.initials: H
authors.lastname: Castillo-Juárez
authors.forename: H
authors.initials: H
authors.lastname: Ramírez-Mendoza
authors.forename: M E
authors.initials: ME
authors.lastname: Trujillo-Ortega
authors.forename: J I
authors.initials: JI
authors.lastname: Sánchez-Betancourt
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood / Q000235:genetics*; D014644:Genetic Variation; D008800:Mexico / Q000453:epidemiology; D019213:Rubulavirus / Q000235:genetics* / Q000276:immunology; D019351:Rubulavirus Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016036:Seroepidemiologic Studies; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral