title: DNA origami nanopores.
abstract: We demonstrate the assembly of functional hybrid nanopores for single molecule sensing by inserting DNA origami structures into solid-state nanopores. In our experiments, single artificial nanopores based on DNA origami are repeatedly inserted in and ejected from solid-state nanopores with diameters around 15 nm. We show that these hybrid nanopores can be employed for the detection of λ-DNA molecules. Our approach paves the way for future development of adaptable single-molecule nanopore sensors based on the combination of solid-state nanopores and DNA self-assembly.
authors.affiliation: Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge, CB3 0HE, United Kingdom.
authors.forename: Nicholas A W
authors.initials: NA
authors.lastname: Bell
authors.forename: Christian R
authors.initials: CR
authors.lastname: Engst
authors.forename: Marc
authors.initials: M
authors.lastname: Ablay
authors.forename: Giorgio
authors.initials: G
authors.lastname: Divitini
authors.forename: Caterina
authors.initials: C
authors.lastname: Ducati
authors.forename: Tim
authors.initials: T
authors.lastname: Liedl
authors.forename: Ulrich F
authors.initials: UF
authors.lastname: Keyser
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D008422:Materials Testing; D015336:Molecular Probe Techniques; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D016062:Porosity; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D004247:DNA