title: The methylthiolation reaction mediated by the Radical-SAM enzymes.
abstract: Over the past 10 years, considerable progress has been made in our understanding of the mechanistic enzymology of the Radical-SAM enzymes. It is now clear that these enzymes appear to be involved in a remarkably wide range of chemically challenging reactions. This review article highlights mechanistic and structural aspects of the methylthiotransferases (MTTases) sub-class of the Radical-SAM enzymes. The mechanism of methylthio insertion, now observed to be performed by three different enzymes is an exciting unsolved problem. This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology.
authors.affiliation: Institut de Recherches en Technologie et Sciences pour le Vivant IRTSV-LCBM, UMR 5249 CEA/CNRS/UJF, CEA-Grenoble, 17 avenue des Martyrs, 38054, Grenoble Cedex 09, France. mohamed.atta@cea.fr
authors.forename: Mohamed
authors.initials: M
authors.lastname: Atta
authors.forename: Simon
authors.initials: S
authors.lastname: Arragain
authors.forename: Marc
authors.initials: M
authors.lastname: Fontecave
authors.forename: Etienne
authors.initials: E
authors.lastname: Mulliez
authors.forename: John F
authors.initials: JF
authors.lastname: Hunt
authors.forename: Jon D
authors.initials: JD
authors.lastname: Luff
authors.forename: Farhad
authors.initials: F
authors.lastname: Forouhar
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism*; D008780:Methyltransferases / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000737:chemistry / Q000378:metabolism*; D013466:Sulfurtransferases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D005609:Free Radicals; D007506:Iron-Sulfur Proteins; D012269:Ribosomal Proteins; C506766:RpsL protein, E coli; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D013466:Sulfurtransferases