title: Daily changes in the phosphoproteome of the dinoflagellate Lingulodinium.
abstract: The dinoflagellate Lingulodinium has a large number of daily rhythms, many of which have no biochemical correlates. We examined the possibility that changes in protein phosphorylation may mediate some of the rhythmic changes by comparing proteins prepared from midday (LD6) and midnight (LD18) cultures. We used two different methods, one a 2D gel protocol in which phosphoproteins were identified after staining with ProQ Diamond, and the other an LC-MS/MS identification of tryptic phosphopeptides that had been purified by TiO(2) chromatography. Two differentially phosphorylated proteins, a light harvesting complex protein and Rad24, were identified using the 2D gel protocol. Six differentially phosphorylated proteins, a polyketide synthase, an uncharacterized transporter, a LIM (actin binding) domain and three RNA binding domain proteins, were identified using the phosphopeptide enrichment protocol. We conclude that changes in protein phosphorylation may underlie some of the rhythmic behavior of Lingulodinium.
authors.affiliation: Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke est, Montréal, Québec, Canada H1X 2B2.
authors.forename: Bolin
authors.initials: B
authors.lastname: Liu
authors.forename: Samuel Chun-Lap
authors.initials: SC
authors.lastname: Lo
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Matton
authors.forename: B Franz
authors.initials: BF
authors.lastname: Lang
authors.forename: David
authors.initials: D
authors.lastname: Morse
mesh_terms: D002853:Chromatography, Liquid; D004141:Dinoflagellida / Q000737:chemistry* / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D010507:Periodicity; D010750:Phosphoproteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D015800:Protozoan Proteins / Q000032:analysis*; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry
chemical_list: D010750:Phosphoproteins; D020543:Proteome; D015800:Protozoan Proteins