title: A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes.
abstract: A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of five acid/alkaline phytohormones, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthylacetic acid (NAA), gibberellic acid (GA(3)) and isopentenyladenine (2IP), in grapes was developed. After optimization, the samples were extracted with methanol containing 1% formic acid and purified by Oasis HLB SPE cartridges. The analytes were separated on a Thermo Hypersil Gold column (100 mm×2.1 mm, 3.0 μm) with water and acetonitrile, then determined with Thermo tandem quadrupole mass spectrometer operating in negative electro-spray ionization using selected reaction monitoring (SRM) mode. The established method was further validated by determining the linearity (R² ≥ 0.9990), average recovery (82.5-105.4%), sensitivity (0.05-1.00 ng mL⁻¹), precision (RSD ≤1 3.0%) and stability (RSD ≥ 82.0%). Finally, the application of the approach proposed to thirty grape samples convinced its desirable performance for rapid analysis of multiclass phytohormones, supporting its sufficient capability for multiresidue analyses or other analytical system targeting phytohormones in agriculture field.
authors.affiliation: Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1018 Jinqi Road, Shanghai 201403, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Qinxiong
authors.initials: Q
authors.lastname: Rao
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification; D004355:Drug Stability; D005875:Gibberellins / Q000032:analysis / Q000302:isolation & purification; D007210:Indoleacetic Acids / Q000032:analysis / Q000302:isolation & purification; D007211:Indoles / Q000032:analysis / Q000302:isolation & purification; D007541:Isopentenyladenosine / Q000032:analysis / Q000302:isolation & purification; D009284:Naphthols / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
chemical_list: D005875:Gibberellins; D007210:Indoleacetic Acids; D007211:Indoles; D009284:Naphthols; D010936:Plant Extracts; D010937:Plant Growth Regulators; C014612:indolebutyric acid; C001478:N(6)-(delta(2)-isopentenyl)adenine; C005366:naphthyl acetate; C030737:indoleacetic acid; D007541:Isopentenyladenosine; C007842:gibberellic acid