title: Olfactomedin 4 defines a subset of human neutrophils.
abstract: OLFM4 was identified initially as a gene highly induced in myeloid stem cells by G-CSF treatment. A bioinformatics method using a global meta-analysis of microarray data predicted that OLFM4 would be associated with specific granules in human neutrophils. Subcellular fractionation of peripheral blood neutrophils demonstrated complete colocalization of OLFM4 with the specific granule protein NGAL, and stimulation of neutrophils with PMA resulted in corelease of NGAL and OLFM4, proving that OLFM4 is a genuine constituent of neutrophil-specific granules. In accordance with this, OLFM4 mRNA peaked at the MY/MM stage of maturation. OLFM4 was, however, present in only 20-25% of peripheral blood neutrophils, as determined by immunocytochemistry and flow cytometry, whereas mRNA for OLFM4 was present in all MY/MM, indicating post-transcriptional regulation as a basis for the heterogeneous expression of OLFM4 protein.
authors.affiliation: Granulocyte Research Laboratory, Rigshospitalet 9322, 20 Juliane Maries vej, Copenhagen, Denmark.
authors.forename: Stine N
authors.initials: SN
authors.lastname: Clemmensen
authors.forename: Christina T
authors.initials: CT
authors.lastname: Bohr
authors.forename: Sara
authors.initials: S
authors.lastname: Rørvig
authors.forename: Andreas
authors.initials: A
authors.lastname: Glenthøj
authors.forename: Helena
authors.initials: H
authors.lastname: Mora-Jensen
authors.forename: Elisabeth P
authors.initials: EP
authors.lastname: Cramer
authors.forename: Lars C
authors.initials: LC
authors.lastname: Jacobsen
authors.forename: Maria T
authors.initials: MT
authors.lastname: Larsen
authors.forename: Jack B
authors.initials: JB
authors.lastname: Cowland
authors.forename: Julia T
authors.initials: JT
authors.lastname: Tanassi
authors.forename: Niels H H
authors.initials: NH
authors.lastname: Heegaard
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Wren
authors.forename: Asli N
authors.initials: AN
authors.lastname: Silahtaroglu
authors.forename: Niels
authors.initials: N
authors.lastname: Borregaard
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D022423:Myeloid Cells / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000145:classification* / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C454127:OLFM4 protein, human; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor