title: Human recombinant Cripto-1 increases doubling time and reduces proliferation of HeLa cells independent of pro-proliferation pathways.
abstract: Human oncofetal protein Cripto-1 (CR-1) is overexpressed in many types of cancers. CR-1 binds to cell surface Glypican-1 to activate Erk1/2 MAPK and Akt pathways leading to cell proliferation. However, we show that treatment with recombinant CR-1 reduces proliferation of HeLa cells by increasing the doubling time without triggering cell death or cell cycle arrest. Using a comparative study with U-87 MG cells, we show that the pro-proliferative pathway of CR-1 is not effective in HeLa cells due to lower expression of Glypican-1. Further we show that treatment with recombinant CR-1 increases PTEN in HeLa cells leading to downregulation of PI3K/Akt pathway. The anti-proliferative effect gets potentiated when the pro-proliferative pathway is blocked.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, India. biplabbose@iitg.ernet.in
authors.forename: Asim Bikas
authors.initials: AB
authors.lastname: Das
authors.forename: Pojul
authors.initials: P
authors.lastname: Loying
authors.forename: Biplab
authors.initials: B
authors.lastname: Bose
mesh_terms: D001483:Base Sequence; D049109:Cell Proliferation; D017931:DNA Primers; D058851:GPI-Linked Proteins / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D009363:Neoplasm Proteins / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D017931:DNA Primers; D058851:GPI-Linked Proteins; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; C070636:TDGF1 protein, human