title: A lateral flow protein microarray for rapid and sensitive antibody assays.
abstract: Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings.
authors.affiliation: Division of Nanobiotechnology, School of Biotechnology, KTH-Royal Institute of Technology, Albanova University Center, 106 91 Stockholm, Sweden;  jesper.gantelius@biotech.kth.se.
authors.forename: Jesper
authors.initials: J
authors.lastname: Gantelius
authors.forename: Tarek
authors.initials: T
authors.lastname: Bass
authors.forename: Ronald
authors.initials: R
authors.lastname: Sjöberg
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.forename: Helene
authors.initials: H
authors.lastname: Andersson-Svahn
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D019540:Area Under Curve; D015415:Biomarkers / Q000032:analysis*; D019295:Computational Biology; D006046:Gold / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D011817:Rabbits
chemical_list: D000906:Antibodies; D015415:Biomarkers; D007074:Immunoglobulin G; D006046:Gold