title: [Alteration of mi-RNA expression profile after interferon treatment in HCV-infected Huh7.5.1 cells].
abstract: To screen the mi-RNA expression profile after interferon treatment in cells infected with hepatitis C virus (HCV).
  
  
  
  Huh-7.5.1 cells was infected with HCV by in vitro transcription and cultured with interferon. The mi-RNA microarray was used to measure the mi-RNA expression in the control group, HCV transcription group and interference group. Intra-group differences were analyzed by the 2 ((-delt delt CT)) method.
  
  
  
  With mi-RNA expressed in normal Huh-7.5.1 cells as a benchmark, expressions of 13 kinds of mi-RNAs were up-regulated after HCV infection and then down-regulated following interferon treatment; 7 were down-regulated after HCV infection and then up-regulated following interferon treatment.
  
  
  
  mi-RNA10a, mi-RNA21, mi-RNA149, mi-RNA152 and mi-RNA210 may be related to hepatitis C virus replication and transcription.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiao-kang
authors.initials: XK
authors.lastname: Xing
authors.forename: Guang-zhong
authors.initials: GZ
authors.lastname: Song
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D016174:Hepacivirus / Q000235:genetics*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007372:Interferons / Q000494:pharmacology; D035683:MicroRNAs / Q000187:drug effects / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014162:Transfection
chemical_list: D016898:Interferon-alpha; D035683:MicroRNAs; D012367:RNA, Viral; D007372:Interferons