title: Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water.
abstract: A novel Gram-negative, rod-shaped, non-motile, non-sporulating bacterium, designated strain K1(T), was isolated from an estuarine water sample collected from Kochi, Kerala, India. Colonies on marine agar were circular, 2.0-2.5 mm in diameter, shiny, yellow, translucent and convex with entire margins. Strain K1(T) was negative for ornithine decarboxylase, lysine decarboxylase, nitrate reduction and H(2)S production. The fatty acids were dominated by iso-branched components with a high abundance of iso-C(15:0), iso-C(15:1) G and iso-C(17:0) 3-OH; MK-6 (64%) and MK-7 (34%) were found as major respiratory quinones; and phosphatidylethanolamine, two unidentified aminolipids, four unidentified phospholipids and two unidentified lipids were major polar lipids. The DNA G+C content of strain K1(T) was 46.1 mol%. 16S rRNA gene sequence analysis indicated that strain K1(T) was related most closely to the type strain of Zhouia amylolytica (pairwise sequence similarity of 93.0%). Phylogenetic analysis showed that strain K1(T) formed a distinct branch within the family Flavobacteriaceae and clustered with the clade comprising species of the genera Zhouia, Coenonia and Capnocytophaga, being phylogenetically most closely related to the type strain of Zhouia amylolytica at a distance of 9.2% (90.8% similarity). Other species of the genera within the same clade were related to strain K1(T) at distances of 15.0-23.1%. Based on phenotypic and chemotaxonomic characteristics and on phylogenetic inference, strain K1(T) is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Imtechella halotolerans gen. nov., sp. nov. is proposed. The type strain of Imtechella halotolerans is K1(T) (=MTCC 11055(T)=JCM 17677(T)).
authors.affiliation: CSIR-Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Surendra
authors.affiliation: CSIR-Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors.forename: Pant
authors.initials: P
authors.lastname: Bhawana
authors.affiliation: CSIR-Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors.forename: Korpole
authors.initials: K
authors.lastname: Suresh
authors.affiliation: CSIR-National Institute of Oceanography, Regional Centre, PB No. 1913, Dr Salim Ali Road, Kochi 682018 (Kerala), India.
authors.forename: T N R
authors.initials: TNR
authors.lastname: Srinivas
authors.affiliation: CSIR-Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India.
authors.forename: Pinnaka
authors.initials: P
authors.lastname: Anil Kumar
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D063366:Estuaries; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007194:India; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S