title: Characterization of T-cell memory phenotype after in vitro expansion of tumor-infiltrating lymphocytes from melanoma patients.
abstract: Memory T-cell populations in human antitumor tumor-infiltrating lymphocytes (TILs) for adoptive cell transfer have not been fully characterized. Our studies demonstrated that CD62L, CD27 and CD28 positive effector memory T-cells were present in the TIL samples from the tumor tissues of melanoma patients and T-cell expansion led to the significant loss of memory T-cells. CD27- and CD28-positive T-cells had high levels of CD44 expression. T-Cell expansion resulted in significant down-regulation of CD44 expression. Interleukin-2 (IL-2) and anti-CD3 antibody stimulation may be responsible for CD44 down-regulation on CD8(+) T-cells during expansion. Furthermore, CD44 down-regulation using small interfering RNA (siRNA) on TILs dramatically reduced interferon-gamma and IL-2 release upon tumor stimulation. These results suggest that the regulation of CD44 expression in TILs may play an important role in memory T-cell maintenance and antitumor immune response.
authors.affiliation: Department of Pathology, School of Medicine, University of South Carolina, 6439 Garners Ferry Road, Columbia, SC 29209, USA. Juhua.Zhou@uscmed.sc.edu
authors.forename: Juhua
authors.initials: J
authors.lastname: Zhou
authors.forename: Prakash
authors.initials: P
authors.lastname: Nagarkatti
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Mitzi
authors.initials: M
authors.lastname: Nagarkatti
mesh_terms: D018106:CD28 Antigens / Q000096:biosynthesis; D017252:CD3 Complex / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D007156:Immunologic Memory; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000378:metabolism; D019041:L-Selectin / Q000096:biosynthesis; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008545:Melanoma / Q000276:immunology*; D034741:RNA, Small Interfering / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D034741:RNA, Small Interfering; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D019041:L-Selectin