title: Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle.
abstract: Mycobacterium bovis isolates from an outbreak of bovine tuberculosis in a herd of cattle in Rio de Janeiro, Brazil, were analysed by spoligotyping and variable-number tandem repeat PCR analysis of the mycobacterial interspersed repetitive unit and exact tandem repeats. Molecular typing revealed a high genetic diversity of strains in the herd. The genetic diversity could be explained by the introduction of infected animals from different sources.
authors.affiliation: Departamento de Alimentos e Nutrição, Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil. figueiredoeduardo@ufmt.br
authors.forename: Eduardo Eustáquio de Souza
authors.initials: EE
authors.lastname: Figueiredo
authors.forename: Daniela Fernandes
authors.initials: DF
authors.lastname: Ramos
authors.forename: Luciana
authors.initials: L
authors.lastname: Medeiros
authors.forename: Flavia Galindo
authors.initials: FG
authors.lastname: Silvestre
authors.forename: Walter
authors.initials: W
authors.lastname: Lilenbaum
authors.forename: Joab Trajano
authors.initials: JT
authors.lastname: Silva
authors.forename: Vânia Margaret Flosi
authors.initials: VM
authors.lastname: Paschoalin
authors.forename: Odir Antônio
authors.initials: OA
authors.lastname: Dellagostin
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D015169:Colony Count, Microbial / Q000662:veterinary; D004269:DNA, Bacterial / Q000235:genetics*; D003612:Dairying; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D018598:Minisatellite Repeats; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial