title: Ultrasound-assisted extraction of oleanolic acid and ursolic acid from Ligustrum lucidum Ait.
abstract: Oleanolic acid and ursolic acid are the main bioactive compounds in fruit of Ligustrum lucidum Ait, which possess anti-inflammatory, antioxidative, antiprotozoal, antimutagenic and anticancer properties. In this study, the ultrasound-assisted extraction of oleanolic acid and ursolic acid from L. lucidum Ait was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, extraction temperature and extraction time, on extraction efficiencies of oleanolic acid and ursolic acid from L. lucidum were evaluated. The influence of experimental parameters on extraction efficiency of ursolic acid was more significant than that of oleanolic acid. The optimal extraction conditions were 95% ethanol, the ratio of material to liquid at 1:20, and extraction for 10 min at 40°C under ultrasonic irradiation. Under the optimal conditions, the yields of oleanolic acid and ursolic acid were 6.3 ± 0.25 and 9.8 ± 0.30 mg/g, respectively. The results indicated that the ultrasound-assisted extraction is a very useful method for the extraction of oleanolic acid and ursolic acid from L. lucidum.
authors.affiliation: Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: En-Qin
authors.initials: EQ
authors.lastname: Xia
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Yu
authors.forename: Xiang-Rong
authors.initials: XR
authors.lastname: Xu
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Deng
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Guo
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Li
mesh_terms: D005638:Fruit / Q000737:chemistry*; D031659:Ligustrum / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013010:Sonication; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D012997:Solvents; D014315:Triterpenes; D000097245:Ursolic Acid