title: Differential interactions of isomeric amino sugars with insulin studied under electrospray ionisation mass spectrometry.
abstract: Protein-ligand interactions were studied for bovine insulin-amino sugar systems under electrospray ionisation mass spectrometry conditions. The isomeric amino sugars showed differences in the relative abundance of 1:1 protein-ligand complex formation. The electrospray ionisation and tandem mass spectrometry results of the complex clearly demonstrated that the differences in the interaction of isomeric sugars with insulin are mainly due to the differences in their gas-phase basicity. The same phenomenon is replicated in the formation of complexes between insulin and other ligands, such as amino acids, as well as in the binding of the amino  sugars with amyloid β 1-40 peptide.
authors.affiliation: National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad-500 607, AP India. nagaveni02@gmail.com
authors.forename: Voleti
authors.initials: V
authors.lastname: Nagaveni
authors.forename: Manyam
authors.initials: M
authors.lastname: Sravani
authors.forename: Sripadi
authors.initials: S
authors.lastname: Prabhakar
authors.forename: Mariappanadar
authors.initials: M
authors.lastname: Vairamani
mesh_terms: D000606:Amino Sugars / Q000737:chemistry / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D005740:Gases; D006031:Glycosylation; D006595:Hexosamines / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000378:metabolism*; D007536:Isomerism; D008024:Ligands; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D000606:Amino Sugars; D016229:Amyloid beta-Peptides; D005740:Gases; D006595:Hexosamines; D007328:Insulin; D008024:Ligands; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)