title: IFN-γ suppresses the high glucose-induced increase in TGF-β1 and CTGF synthesis in mesangial cells.
abstract: Mesangial cells are the main source of renal interstitial fibrosis in diabetic nephropathy (DN). Interferon-γ (IFN-γ) is a key cytokine that may play a potential therapeutic role in reducing fibrosis. Here, we focus on the effects of IFN-γ on human mesangial cells (HMCs) treated with high glucose. This study shows that IFN-γ phosphorylates STAT1, suppresses HMC proliferation, and downregulates mRNA and protein levels of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in HMCs treated with high glucose. The regulation of P-STAT1 could change HMC proliferation and the expression of fibrotic cytokines TGF-β1 and CTGF in HMCs. These data indicate that IFN-γ could activate STAT1 to suppress the increase in TGF-β1 and CTGF synthesis in HMCs induced by high glucose. This paper may lead to new therapeutic treatments of DN.
authors.affiliation: Department of Nephrology, First Hospital of China Medical University, 155 North Nanjing Street, Shenyang City, Liaoning Province 110001, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Lining
authors.initials: L
authors.lastname: Wang
authors.forename: Linlin
authors.initials: L
authors.lastname: Liu
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Ping
authors.initials: P
authors.lastname: Kang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000096:biosynthesis*; D003928:Diabetic Nephropathies / Q000188:drug therapy / Q000503:physiopathology; D015536:Down-Regulation / Q000187:drug effects; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D050527:Mesangial Cells / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D050794:STAT1 Transcription Factor; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: C526317:CCN2 protein, human; D012333:RNA, Messenger; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor; D007371:Interferon-gamma; D005947:Glucose