title: Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.
abstract: We developed a general restriction enzyme-free and ligase-free method for subcloning up to three DNA fragments into any location of a plasmid. The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids.
authors.affiliation: Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Chun
authors.initials: C
authors.lastname: You
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Y-H Percival
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial