title: Prevalence of antibodies anti-Bartonella henselae in western Sicily: children, blood donors, and cats.
abstract: To evaluate seroprevalence of B. henselae infection both in Sicilian children and healthy blood donors. Furthermore, circulation of Bartonella in the natural reservoir was also studied. Two hundred forty-three children, living in Sicily (Palermo), affected by various diseases, without clinical features suggesting B. henselae infection, together with 122 healthy blood donors were serologically investigated for IgG and IgM antibodies by indirect fluorescent antibody test (IFAT). One hundred twenty stray and 62 pet cats were also analyzed only for IgG. Among children 25.1% had IgG antibodies to B. henselae; 18.5% showed a titer 1:64, 2.4% 1:128, 2.4% 1:256, 0.8% 1:512, 0.4% 1:1024, and 0.4% 1:5120. Among healthy blood donors 11.4% had IgG class antibodies to B. henselae; 9.8% showed a titer 1:64 and 1.6% 1:128. All the human serum samples did not show positive results for B. henselae IgM class antibodies. Stray cats (68.3%) and pet cats (35.4%) also had IgG class antibodies to B. henselae. We demonstrated high frequency of serologic evidence of past B. henselae infection, in young Italian children, affected by various diseases, apparently free of any clinical features suggesting B. henselae infection. This observation is supported by high circulation of Bartonella in cats.
authors.affiliation: Dipartimento di Medicina Clinica e delle Patologie Emergenti, University of Palermo, Palermo, Italy. mansueto@unipa.it
authors.forename: Pasquale
authors.initials: P
authors.lastname: Mansueto
authors.forename: Ilenia
authors.initials: I
authors.lastname: Pepe
authors.forename: Enrico
authors.initials: E
authors.lastname: Cillari
authors.forename: Francesco
authors.initials: F
authors.lastname: Arcoleo
authors.forename: Anna
authors.initials: A
authors.lastname: Micalizzi
authors.forename: Floriana
authors.initials: F
authors.lastname: Bonura
authors.forename: Aurelio
authors.initials: A
authors.lastname: Seidita
authors.forename: Letizia
authors.initials: L
authors.lastname: Palillo
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Di Gregorio
authors.forename: Mario
authors.initials: M
authors.lastname: Affronti
authors.forename: Salvatore
authors.initials: S
authors.lastname: Di Rosa
authors.forename: Giovambattista
authors.initials: G
authors.lastname: Rini
authors.forename: Giustina
authors.initials: G
authors.lastname: Vitale
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D018416:Bartonella henselae / Q000276:immunology*; D001782:Blood Donors; D002415:Cats / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007223:Infant; D008297:Male; D008875:Middle Aged; D012802:Sicily / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M