title: Quantitative determination and pharmacokinetic study of casticin in rat plasma by liquid chromatography-mass spectrometry.
abstract: A specific and sensitive liquid chromatography-mass spectrometry method has been developed and validated for identification and quantification of casticin in rat plasma after oral and intravenous administrations. Kaempferol was employed as an internal standard (IS). Liquid-liquid extraction using dichloromethane was applied to extract the casticin and the internal standard from plasma. Chromatographic separation was achieved on a Zorbax SB C(18) column (100 mm × 3.0 mm, i.d.: 3.5 μm) with a mobile phase of methanol: 0.05% formic acid aqueous solution (60:40, v/v) at a flow rate of 0.4 mL/min for 10 min. The detection was performed by selected ion monitoring (SIM) mode via positive electrospray ionization (ESI) interface. The lower limit of detection (LLOD) was 7 ng/mL (S/N=5). The calibration curves were linear (r>0.9996) over the concentration range from 14.06 to 7187 ng/mL. Intra-day and inter-day precisions (RSD%) were within 10% and accuracy (RE%) ranged from -8.2% to 1.0%. The extraction recovery at three quality control (QC) concentrations ranged from 91% to 102%. The validated method was successfully applied to the pharmacokinetic study of casticin after both an oral and an intravenous administrations to rats and the absolute bioavailability is 45.5 ± 11.0%.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Xu
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Liming
authors.initials: L
authors.lastname: Xue
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Chengjian
authors.initials: C
authors.lastname: Zheng
authors.forename: Luping
authors.initials: L
authors.lastname: Qin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D005419:Flavonoids / Q000097:blood* / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D005419:Flavonoids; C054133:casticin