title: Indirect inactivation of tyrosinase in its action on tyrosine.
abstract: Under aerobic conditions, tyrosinase is inactivated by dopa as a result of suicide inactivation, and, under anaerobic conditions, as a result of irreversible inactivation. However, tyrosine protects the enzyme from being inactivated by dopa under anaerobic conditions. This paper describes how under aerobic conditions the enzyme acting on tyrosine is not directly inactivated but undergoes a process of indirect suicide inactivation provoked by reaction with the o-diphenol originated from the evolution of o-dopaquinone and accumulated in the reaction medium.
authors.affiliation: GENZ: Grupo de Investigación Enzimología, Departamento de Bioquímica y Biología Molecular-A, Universidad de Murcia, Espinardo, Murcia, Spain.
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Muñoz-Muñoz
authors.forename: Francisco
authors.initials: F
authors.lastname: Garcia-Molina
authors.forename: Jose Ramon
authors.initials: JR
authors.lastname: Acosta-Motos
authors.forename: Enrique
authors.initials: E
authors.lastname: Arribas
authors.forename: Pedro Antonio
authors.initials: PA
authors.lastname: Garcia-Ruíz
authors.forename: Jose
authors.initials: J
authors.lastname: Tudela
authors.forename: Francisco
authors.initials: F
authors.lastname: Garcia-Cánovas
authors.forename: Jose Neptuno
authors.initials: JN
authors.lastname: Rodríguez-López
mesh_terms: D000363:Agaricales / Q000201:enzymology; D016227:Benzoquinones / Q000378:metabolism; D003198:Computer Simulation; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D004789:Enzyme Activation; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D013237:Stereoisomerism; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D016227:Benzoquinones; D004791:Enzyme Inhibitors; D010636:Phenols; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; C035157:dopaquinone; D010088:Oxidoreductases; C412191:monophenolase; D014442:Monophenol Monooxygenase; D010100:Oxygen