title: Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.
abstract: A quantitative PCR procedure targeting the β-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Kurt
authors.initials: K
authors.lastname: Showmaker
authors.forename: Gary W
authors.initials: GW
authors.lastname: Lawrence
authors.forename: Shien
authors.initials: S
authors.lastname: Lu
authors.forename: Clarissa
authors.initials: C
authors.lastname: Balbalian
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Klink
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D018509:DNA, Helminth / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification*; D040362:Geographic Information Systems; D056186:Metagenomics / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012015:Reference Standards; D012987:Soil / Q000469:parasitology*; D014431:Tylenchoidea / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D012987:Soil