title: [Effect of cyclosporine on regulatory T cells and Foxp3 in the peripheral blood of children with chronic aplastic anemia].
abstract: OBJECTIVE
  To explore the expression diversification of CD4(+)CD25(+)CD127(low) regulatory T (Treg) cells and Foxp3 mRNA in the peripheral blood of children with aplastic anemia after the treatment with cyclosporine.
  
  
  METHODS
  Fifty children with chronic aplastic anemia were enrolled, among whom 30 received cyclosporine treatment (cyclosporine group) and 20 were treated with conventional methods (conventional group). Twenty healthy children were enrolled as the control group. The expression of CD4(+)CD25(+)CD127(low) Treg cells was detected by flow cytometry. The expression of Foxp3 mRNA was detected by real-time Q-PCR.
  
  
  RESULTS
  The expressions of Foxp3 mRNA and CD4(+)CD25(+)CD127(low)Treg cells showed no significant difference between the cyclosporine and the control groups 6 months after treatment. On the contrary, there were significantly lower expressions of both in the conventional group than in the control group (P<0.05). Meanwhile, the cyclosporine group had significantly higher expressions of Foxp3 mRNA and CD4(+)CD25(+)CD127(low) Treg cells than the conventional group (P<0.05).
  
  
  CONCLUSIONS
  The expressions of CD4(+)CD25(+)CD127(low) Treg cells and Foxp3 mRNA in children with aplastic anemia increase after cyclosporine treatment.
authors.affiliation: Department of Pediatrics, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. xigewang1962@sina.com
authors.forename: Xi-Ge
authors.initials: XG
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Xiao-Ge
authors.initials: XG
authors.lastname: Wang
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Qiao
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Cao
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000741:Anemia, Aplastic / Q000188:drug therapy* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D016572:Cyclosporine / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D051858:Forkhead Transcription Factors / Q000097:blood* / Q000235:genetics; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000097:blood; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D007166:Immunosuppressive Agents; D012333:RNA, Messenger; D016572:Cyclosporine