title: A HNMR-based metabonomics study of postmenopausal osteoporosis and intervention effects of Er-Xian Decoction in ovariectomized rats.
abstract: A metabonomics method using (1)H nuclear magnetic resonance spectroscopy ((1)HNMR) was applied to obtain a systematic view of the development and progression of postmenopausal osteoporosis. Using partial least squares discriminant analysis (PLS-DA), 26 and 34 characteristic resonances were found respectively in urine and plasma of ovariectomized rats (Variable importance, VIP value ≥1.0), and the significant altered metabolites identified in the plasma and urine were 10 and 9, respectively. Changes in these metabolites were related to the pathways of lipid, energy and amino acid metabolism, some of which involved the oxidative system. The described method was also used to analyze the therapeutic effects of Er-Xian Decoction (EXD), a traditional Chinese medicine widely used in the clinical treatment of osteoporosis in China. The results showed that EXD administration could provide satisfactory effects on osteoporosis through partially regulating the perturbed pathways of lipid, energy and amino acid metabolism and improving the anti-oxidative ability.
authors.affiliation: School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China; E-Mails:  lm.xue@hotmail.com  (L.X.);  337589971@qq.com  (L.L.);  zhaolu850813@163.com  (L.Z.);  hantinglh1@hotmail.com  (T.H.).
authors.forename: Liming
authors.initials: L
authors.lastname: Xue
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhao
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Qiaoyan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Luping
authors.initials: L
authors.lastname: Qin
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; C517980:er-xian decoction