title: Specification of the cholesterol interaction with the oxytocin receptor using a chimeric receptor approach.
abstract: The oxytocin receptor specifically requires cholesterol to maintain and stabilize its high-affinity agonist binding. Here, we applied a receptor chimeric approach to coarsely localize the cholesterol binding domain of the oxytocin receptor. During these studies, we identified the specific dependence on cholesterol as a common property of the oxytocin-vasopressin receptor family. We asked whether the oxytocin receptor maintains or loses its cholesterol dependence when parts of the receptor are exchanged by the corresponding fragments of the cholecystokinin receptor that does not show a specific cholesterol dependence. One of the chimeric receptors revealed full oxytocin binding activity, was capable of signal transduction, and its cholesterol dependence was nearly indistinguishable from that of the oxytocin receptor itself. The data suggest that at least the C-terminal segments of the oxytocin receptor including transmembrane domains 6 and 7 do not participate in cholesterol binding. Thus, the binding sites for the modulator cholesterol and for the agonist oxytocin are therefore most likely colocalized in the N-terminal segment of the oxytocin receptor.
authors.affiliation: Institute of Pharmacy and Biochemistry, Department of Biochemistry, Johann-Joachim-Becherweg 30, 55099 Mainz, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Wiegand
authors.forename: Gerald
authors.initials: G
authors.lastname: Gimpl
mesh_terms: D000595:Amino Acid Sequence; D002784:Cholesterol / Q000378:metabolism*; D002789:Cholesterol Oxidase / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects; D018045:Receptors, Oxytocin / Q000737:chemistry / Q000378:metabolism*; D017483:Receptors, Vasopressin / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D011993:Recombinant Fusion Proteins; D002784:Cholesterol; D002789:Cholesterol Oxidase