title: Novel multiplexed HIV/simian immunodeficiency virus antibody detection assay.
abstract: Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.
authors.affiliation: University of Montpellier, Montpellier, France.
authors.forename: Steve
authors.initials: S
authors.lastname: Ahuka-Mundeke
authors.forename: Ahidjo
authors.initials: A
authors.lastname: Ayouba
authors.forename: Placide
authors.initials: P
authors.lastname: Mbala-Kingebeni
authors.forename: Florian
authors.initials: F
authors.lastname: Liegeois
authors.forename: Amandine
authors.initials: A
authors.lastname: Esteban
authors.forename: Octavie
authors.initials: O
authors.lastname: Lunguya-Metila
authors.forename: Didace
authors.initials: D
authors.lastname: Demba
authors.forename: Guy
authors.initials: G
authors.lastname: Bilulu
authors.forename: Valentin
authors.initials: V
authors.lastname: Mbenzo-Abokome
authors.forename: Bila-Isia
authors.initials: BI
authors.lastname: Inogwabini
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Muyembe-Tamfum
authors.forename: Eric
authors.initials: E
authors.lastname: Delaporte
authors.forename: Martine
authors.initials: M
authors.lastname: Peeters
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D003104:Colobus; D021821:Communicable Diseases, Emerging / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary; D003429:Cross Reactions; D043542:DNA, Z-Form / Q000235:genetics; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D014644:Genetic Variation; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000235:genetics; D015658:HIV Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates / Q000821:virology; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology; D016036:Seroepidemiologic Studies; D016097:Simian Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000635:transmission; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D015047:Zoonoses / Q000453:epidemiology / Q000635:transmission
chemical_list: D000914:Antibodies, Viral; D043542:DNA, Z-Form; D015483:HIV Antibodies; D015488:HIV Antigens; D012191:Retroviridae Proteins