title: Purification and characterization of antibacterial compounds of Pseudoalteromonas flavipulchra JG1.
abstract: Pseudoalteromonas flavipulchra JG1 produces a protein PfaP and a range of small-molecule compounds with inhibitory activities against Vibrio anguillarum. The PfaP protein was purified from the extracellular products of JG1 by electroelution, and antibacterial activity was observed by an in-gel antibacterial assay. The complete amino acid sequence (694 aa) of PfaP was determined by de novo peptide sequencing and subsequent alignment with the proteome sequence of strain JG1. The calculated molecular mass of PfaP was 77.0 kDa. PfaP was 58 % identical to l-lysine oxidase AlpP of Pseudoalteromonas tunicata D2, and 54 % identical to the marinocine antimicrobial protein of Marinomonas mediterranea MMB-1. Five small molecules (compounds 1-5) with antibacterial activity, which were identified as p-hydroxybenzoic acid (1), trans-cinnamic acid (2), 6-bromoindolyl-3-acetic acid (3), N-hydroxybenzoisoxazolone (4) and 2'-deoxyadenosine (5), were purified by sequential column chromatography over silica gel, Sephadex LH-20 and RP-18 from ethyl acetate extract of strain JG1, and their structures were determined by NMR and MS. Brown compound 3, the only brominated compound, showed antibacterial activity against both Gram-positive and Gram-negative bacteria.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Kaihao
authors.initials: K
authors.lastname: Tang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiaochong
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Shushan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Zhu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D002853:Chromatography, Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D031161:Pseudoalteromonas / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial