title: The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes.
abstract: Nuclear pore complexes (NPCs) are large macromolecular assemblies that control all transport across the nuclear envelope. They are formed by about 30 nucleoporins (Nups), which can be roughly categorized into those forming the structural skeleton of the pore and those creating the central channel and thus providing the transport and gating properties of the NPC. Here we show that the conserved nucleoporin Nup93 is essential for NPC assembly and connects both portions of the NPC. Although the C-terminal domain of the protein is necessary and sufficient for the assembly of a minimal structural backbone, full-length Nup93 is required for the additional recruitment of the Nup62 complex and the establishment of transport-competent NPCs.
authors.affiliation: Friedrich Miescher Laboratory of the Max Planck Society, 72076 Tuebingen, Germany.
authors.forename: Ruchika
authors.initials: R
authors.lastname: Sachdev
authors.forename: Cornelia
authors.initials: C
authors.lastname: Sieverding
authors.forename: Matthias
authors.initials: M
authors.lastname: Flötenmeyer
authors.forename: Wolfram
authors.initials: W
authors.lastname: Antonin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008562:Membrane Glycoproteins / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism* / Q000648:ultrastructure; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014981:Xenopus; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D028861:Nuclear Pore Complex Proteins; C109329:Nup93 protein, Xenopus; D029867:Xenopus Proteins; C049312:nuclear pore protein p62