title: Development of miconazole nitrate containing chitosan microcapsules and their anti-Aspergillus niger activity.
abstract: In this article, we report the development of chitosan/miconazole nitrate microcapsules. Four miconazole nitrate ratios including 12.5, 25, 50 and 100 mg were performed in the chitosan-based microencapsulation system. Chitosan microcapsules with the drug input of 25 mg showed the highest encapsulation efficiency (52.47%) and acceptable mean particle size (5.65 µm) when compared with those of 12.5, 50 and 100 mg. Fourier transform infrared spectroscopic spectrum proved the entrapment of miconazole nitrate into chitosan microcapsules. The antifungal result demonstrated that microcapsules containing 75 µg miconazole nitrate possessed comparable anti-Aspergillus niger activity as the commercial ointment. The growth inhibition of miconazole nitrate containing chitosan microcapsules towards human skin keratinocytes was found to be dose dependent. A total of 75 µg of miconazole nitrate containing microcapsules revealed about 25% of growth inhibition while that of 150 µg showed approximately 70% of growth inhibition. Special monitoring should be taken if a higher dose of miconazole nitrate was used to develop the microcapsules.
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong, China.
authors.forename: C W M
authors.initials: CW
authors.lastname: Yuen
authors.forename: C W
authors.initials: CW
authors.lastname: Kan
authors.forename: K L
authors.initials: KL
authors.lastname: Cheuk
authors.forename: H C
authors.initials: HC
authors.lastname: Cheung
authors.forename: S Y
authors.initials: SY
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Yip
authors.forename: P L
authors.initials: PL
authors.lastname: Lam
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001228:Aspergillosis / Q000188:drug therapy; D001234:Aspergillus niger / Q000187:drug effects*; D002214:Capsules / Q000737:chemistry*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004339:Drug Compounding; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D008825:Miconazole / Q000008:administration & dosage* / Q000494:pharmacology; D010316:Particle Size
chemical_list: D000935:Antifungal Agents; D002214:Capsules; D008825:Miconazole; D048271:Chitosan