title: Mitochondrial D-loop variations in infertile women undergoing a long stimulation protocol.
abstract: OBJECTIVE
  To study a high frequency of mtDNA D-loop variations in infertile women undergoing a long stimulation protocol and their potential relevance with endpoints of IVF.
  
  
  METHODS
  Peripheral blood was taken from 156 patient ≤ 42 years of age. The entire D-loop region of mtDNA was amplified in three overlapping polymerase chain reaction fragments, and variations were evaluated by direct DNA sequencing methods in 156 infertile women undergoing a long stimulation protocol.
  
  
  RESULTS
  A total of 48 variations were found at 47 positions in the D-loop of all patients. Median age of the patients was 34.09 years (26-42 years). The incidence of variations was significantly higher in the group of patients aged >34.09 years (P=0.001), especially 16191 C → T (P=0.017) and 199 T → C (P=0.045). In contrast, the incidence of variations was significantly lower on the day of hCG administration in the group of patients with E₂>8037.97 pmol/L (P=0.001). However, variations were not significantly associated with early follicular phase FSH (P=0.262), the number of oocytes retrieved (P=0.191) or the pregnancy rate (P=0.487).
  
  
  CONCLUSIONS
  Our data suggest that the increase in mtDNA variations in peripheral blood from infertile women could have a predictive value for the response of infertile women undergoing a long stimulation protocol.
authors.affiliation: Reproductive Medicine Center of the First Affiliated Hospital of Wenzhou Medical College, 2 Fu Xue Lane, 325000 Wenzhou, Zhejiang Province, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lin
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Sun
mesh_terms: D000328:Adult; D000367:Age Factors; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology; D015331:Cohort Studies; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004351:Drug Resistance; D004958:Estradiol / Q000097:blood; D005260:Female; D056726:Genetic Association Studies; D014644:Genetic Variation; D006771:Hospitals, Maternity; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000235:genetics* / Q000628:therapy; D059372:Nucleotide Motifs; D010060:Ovulation / Q000187:drug effects; D010062:Ovulation Induction; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018873:Pregnancy Rate; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D004272:DNA, Mitochondrial; D004958:Estradiol