title: Decellularized human dermis to treat massive rotator cuff tears: in vitro evaluations.
abstract: Interest is increasing in biological scaffolds for tissue regeneration such as extracellular matrix membranes, developed through soft tissue decellularization. Extracellular matrix membranes were developed to heal different tendon and soft tissue lesions that are very frequent in the general population with high health-care costs and patient morbidity. The aim of this research was to evaluate a human dermal matrix (HDM) decellularized by a chemico-physical method. A primary culture of rat tenocytes was performed: tenocytes were seeded on HDM samples and on polystyrene wells as controls (CTR). Cell viability and synthetic activity were evaluated at 3 and 7 days. An in vitro microwound model was used to evaluate HDM bioactivity: after tenocyte expansion, artificial wounds were created, HDM extracts were added, and closure time and decorin synthesis were monitored histomorphometrically at 1, 4, 24, and 72 hr. A significant higher amount of collagen I was observed when cells were cultured on HDM in comparison with that on CTR (3 days: p < 0.0001; 7 days: p < 0.05). In HDM group, fibronectin synthesis was significantly higher at both experimental times (p < 0.0001). At 3 days, proteoglycans and transforming growth factor-β1 releases were significantly higher on HDM (p < 0.0001 and p < 0.005, respectively). The artificial microwound closure time and decorin expression were significantly enhanced by the addition of 50% HDM extract (p < 0.05). In vitro data showed that the decellularization technique enabled the development of a matrix with adequate biological and biomechanical properties.
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopaedic Institute, Bologna, Italy. milena.fini@ior.it
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Elena
authors.initials: E
authors.lastname: Bondioli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Castagna
authors.forename: Paola
authors.initials: P
authors.lastname: Torricelli
authors.forename: Gianluca
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Roberto
authors.initials: R
authors.lastname: Rotini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Marinelli
authors.forename: Enrico
authors.initials: E
authors.lastname: Guerra
authors.forename: Catuscia
authors.initials: C
authors.lastname: Orlandi
authors.forename: Andrea
authors.initials: A
authors.lastname: Carboni
authors.forename: Annalisa
authors.initials: A
authors.lastname: Aiti
authors.forename: Elisa
authors.initials: E
authors.lastname: Benedettini
authors.forename: Roberto
authors.initials: R
authors.lastname: Giardino
authors.forename: Davide
authors.initials: D
authors.lastname: Melandri
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D058575:Decorin / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D005069:Evaluation Studies as Topic; D006801:Humans; D022125:Lacerations / Q000473:pathology* / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017006:Rotator Cuff / Q000473:pathology; D000070636:Rotator Cuff Injuries; D013537:Sutures; D014945:Wound Healing
chemical_list: D058575:Decorin