title: Structure of the O-polysaccharide chain of the lipopolysaccharide of Psychrobacter muricolla 2pS(T) isolated from overcooled water brines within permafrost.
abstract: Psychrotrophic bacteria of the genus Psychrobacter have not been studied in respect to lipopolysaccharide structure. In this work, we determined the structure of the O-specific polysaccharide of the lipopolysaccharide of Psychrobacter muricolla 2pS(T) isolated from overcooled (-9°C) water brines within permafrost. The polysaccharide was found to be acidic due to the presence of an amide of 2-acetamido-2-deoxy-l-guluronic acid with glycine (l-GulNAcA6Gly), which has not been hitherto found in nature. The following structure of the disaccharide repeating unit of the polysaccharide was established using composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-α-l-GulpNAcA6Gly-(1→3)-β-d-GlcpNAc-(1→
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia. annakond@gmail.com
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.forename: Kseniya A
authors.initials: KA
authors.lastname: Novototskaya-Vlasova
authors.forename: Marina S
authors.initials: MS
authors.lastname: Drutskaya
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Shcherbakova
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: David A
authors.initials: DA
authors.lastname: Gilichinsky
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Nedospasov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D044096:Psychrobacter / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D014867:Water