title: Sequence type ST131 and ST10 complex (ST617) predominant among CTX-M-15-producing Escherichia coli isolates from Nigeria.
abstract: Of 109 clinical Escherichia coli isolates from two major tertiary hospitals in Lagos (University Teaching Hospital and the National Orthopaedic Hospital Igbobi), 14 (12.8%) extended-spectrum beta-lactamse (ESBL) producers were characterized using PCR and sequencing, ERIC-PCR and multilocus sequence typing. All ESBL-producing isolates encoded only the CTX-M-15 gene. Clonal group ST131 (35.7%) was the predominant ST, followed by ST617 (28.6%). Isolated cases of other sequence types were also observed. Plasmid-mediated quinolone resistance genes qnrA, qnrB1 and aac-(6')-lb-cr were detected among these ESBL isolates of different clonal groups. This is the first description of the clonality of CTX-M-15-producing E. coli from Nigeria. The presence of diverse clonal lineages shows the continuing potential for genetic diversification and emergence of new epidemic strains.
authors.affiliation: Department of Medical Microbiology and Parasitology, University of Lagos, Lagos, Nigeria.
authors.forename: I
authors.initials: I
authors.lastname: Aibinu
authors.forename: T
authors.initials: T
authors.lastname: Odugbemi
authors.forename: W
authors.initials: W
authors.lastname: Koenig
authors.forename: B
authors.initials: B
authors.lastname: Ghebremedhin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D009549:Nigeria / Q000453:epidemiology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones; D047090:beta-Lactams; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases