title: Dual-medium quantitative measurement simulation on cells.
abstract: For research on inhomogeneous cells, we present a simulation method called the dual-medium quantitative (DMQ) measurement simulation method, which is realized by combining phase-shifting digital holography with DMQ analysis. The reliability of this method is confirmed by comparing the simulated phase map with the experimental one by the Hilbert phase microscope [J. Phys. Chem. A 113, 13327 (2009)10.1021/jp904746r], and its ability for studying inhomogeneous cells is demonstrated with measurements of a simulated HeLa cell. The average deviation and the relative deviation of physical thickness and axially averaged refractive index are 0.0339 μm, 0.69% and 0.0013, 0.094%, respectively. This approach can provide good guidance for experimental research on inhomogeneous cells.
authors.affiliation: School of Mechanical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.forename: Weifeng
authors.initials: W
authors.lastname: Jin
authors.forename: Naifei
authors.initials: N
authors.lastname: Ren
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D006367:HeLa Cells; D006696:Holography / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D012031:Refractometry / Q000379:methods; D015203:Reproducibility of Results