title: Engineered combined-positive-control template for real-time reverse transcription-PCR in multiple-pathogen-detection assays.
abstract: Recently we evaluated a custom TaqMan array card (TAC) detection system, formerly known as a TaqMan low-density array (TLDA) card, for simultaneous real-time PCR identification of 21 pathogens and three control targets in duplicate from respiratory specimens (M. Kodani et al., J. Clin. Microbiol. 49:2175-2182, 2011). We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR.
authors.affiliation: Division of Bacterial Diseases, Respiratory Diseases Branch, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention,Atlanta, Georgia, USA.
authors.forename: Maja
authors.initials: M
authors.lastname: Kodani
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Winchell
mesh_terms: D006801:Humans; D008828:Microbiological Techniques / Q000592:standards*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000592:standards; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*