title: Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5.
abstract: Botulinum neurotoxins (BoNTs) cause botulism by cleaving proteins necessary for nerve transmission. There are seven serotypes of BoNT, A-G, characterized by their response to antisera. Many serotypes are further distinguished into differing subtypes based on amino acid sequence, some of which result in functional differences. Our laboratory previously reported that all tested subtypes within each serotype have the same site of enzymatic activity. Recently, three new subtypes of BoNT/F; /F3, /F4, and /F5, were reported. Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. This is the first report of cleavage of synaptobrevin-2 in this location.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA.
authors.forename: Suzanne R
authors.initials: SR
authors.lastname: Kalb
authors.forename: Jakub
authors.initials: J
authors.lastname: Baudys
authors.forename: Robert P
authors.initials: RP
authors.lastname: Webb
authors.forename: Patrick
authors.initials: P
authors.lastname: Wright
authors.forename: Theresa J
authors.initials: TJ
authors.lastname: Smith
authors.forename: Leonard A
authors.initials: LA
authors.lastname: Smith
authors.forename: Rafael
authors.initials: R
authors.lastname: Fernández
authors.forename: Brian H
authors.initials: BH
authors.lastname: Raphael
authors.forename: Susan E
authors.initials: SE
authors.lastname: Maslanka
authors.forename: James L
authors.initials: JL
authors.lastname: Pirkle
authors.forename: John R
authors.initials: JR
authors.lastname: Barr
mesh_terms: D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D060066:Protein Interaction Maps; D059748:Proteolysis; D013379:Substrate Specificity / Q000235:genetics; D050988:Vesicle-Associated Membrane Protein 2 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050988:Vesicle-Associated Membrane Protein 2; D010447:Peptide Hydrolases; D001905:Botulinum Toxins; C096321:botulinum toxin type F