title: Flavobacterium ummariense sp. nov., isolated from hexachlorocyclohexane-contaminated soil, and emended description of Flavobacterium ceti Vela et al. 2007.
abstract: A Gram-negative, strictly aerobic, yellow bacterial strain, designated DS-12(T), was isolated from hexachlorocyclohexane-contaminated soil in Lucknow, Uttar Pradesh, India. Strain DS-12(T) showed the highest 16S rRNA gene sequence similarity with Flavobacterium ceti 454-2(T) (94.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-12(T) belonged to the genus Flavobacterium. Strain DS-12(T) produced flexirubin-type pigments. Gliding motility was not observed. The major fatty acids of strain DS-12(T) were iso-C(15:0) (48.0%), summed feature 9 (comprising iso-C(17:1)ω9c and/or C(16:0) 10-methyl; 19.3%), iso-C(17:0) 3-OH (8.5%) and summed feature 3 (comprising one or more of C(16:1)ω7c, C(16:1)ω6c and iso-C(15:0) 2-OH; 7.2%). The only respiratory quinone was menaquinone-6 and the major polyamine was homospermidine. Strain DS-12(T) contained phosphatidyldimethylethanolamine, phosphatidylserine, phosphatidylethanolamine, one unknown phospholipid and one unknown aminolipid. The DNA G+C content was 37.4 mol%. Phylogenetic inference and phenotypic properties indicated that strain DS-12(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ummariense sp. nov. is proposed. The type strain is DS-12(T) (=CCM 7847(T) =MTCC 10766(T)). An emended description of Flavobacterium ceti is also given.
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110 007, India.
authors.forename: Pushp
authors.initials: P
authors.lastname: Lata
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110 007, India.
authors.forename: Devi
authors.initials: D
authors.lastname: Lal
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110 007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001556:Hexachlorocyclohexane / Q000032:analysis; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D001556:Hexachlorocyclohexane