title: Diet-induced obesity up-regulates the abundance of GPR43 and GPR120 in a tissue specific manner.
abstract: OBJECTIVE
  GPR43 and GPR120 have recently been deorphanised as receptors for fatty acids. Fatty acids mediate a variety of metabolic processes in the body, however, the effect these receptors have on metabolism is not fully understood. Here, we characterise the effect of diet-induced obesity on the expression of GPR43 and GPR120 in tissues important in maintaining metabolic health.
  
  
  METHODS
  Six-week old male Sprague Dawley rats were fed either a high fat diet (HFD; 22% fat) or control diet (5% fat; n = 8-9/group) for 12 weeks. Rats were euthanized and the heart, liver, soleus and extensor digitorum longus (EDL) skeletal muscles were excised. GPR43 and GPR120 receptor abundance was quantified by 'real-time' PCR.
  
  
  RESULTS
  GPR43 mRNA abundance was significantly up-regulated by a HFD in liver and soleus and EDL skeletal muscles compared to control (p ≤ 0.05). Whilst a HFD significantly up-regulated GPR120 gene transcripts in cardiac tissue and EDL skeletal muscle when compare to control (p ≤ 0.05).
  
  
  CONCLUSIONS
  We have shown for the first time that up-regulation of GPR43 and GPR120 in response to a HFD, is tissue specific. This suggests these receptors have different roles in mediating metabolic function in a number of tissues in the human body.
authors.affiliation: Biomedical and Lifestyle Diseases Unit, School of Biomedical and Health Sciences, Victoria University, Melbourne, Australia.
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Cornall
authors.forename: Michael L
authors.initials: ML
authors.lastname: Mathai
authors.forename: Deanne H
authors.initials: DH
authors.lastname: Hryciw
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McAinch
mesh_terms: D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009765:Obesity / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C518630:Ffar2 protein, rat; C533284:Ffar4 protein, rat; D043562:Receptors, G-Protein-Coupled