title: A role for inflammatory mediators in the modulation of the neurotrophin receptor p75NTR on human muscle precursor cells.
abstract: The neurotrophin receptor p75NTR is a marker for human differentiation-prone muscle satellite cells and regulates myogenesis. Here, we wondered whether inflammation could modify p75NTR expression on muscle precursor cells in vitro and in vivo. In vitro experiments on human primary skeletal myoblasts demonstrated that exposure to IFN-γ or IL-1α decreased transcript and protein levels of p75NTR. Furthermore, histological investigations showed a reduction in the pool of p75NTR expressing satellite cells in inflammatory myopathy biopsies. These data suggest a link between muscle inflammation and reduction of p75NTR expression on precursor cells.
authors.affiliation: Neuroimmunology and Neuromuscular Disorders Unit, Foundation IRCCS Neurological Institute Carlo Besta, Milan, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Colombo
authors.forename: Stefania
authors.initials: S
authors.lastname: Romaggi
authors.forename: Marina
authors.initials: M
authors.lastname: Mora
authors.forename: Lucia
authors.initials: L
authors.lastname: Morandi
authors.forename: Cinthia
authors.initials: C
authors.lastname: Farina
mesh_terms: D000704:Analysis of Variance; D019002:CD56 Antigen / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D009132:Muscles / Q000166:cytology; D032446:Myoblasts / Q000187:drug effects*; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D019002:CD56 Antigen; D018836:Inflammation Mediators; C522918:NCAM1 protein, human; D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor