title: A novel tyrosinase biosensor based on chitosan-carbon-coated nickel nanocomposite film.
abstract: A novel nanocomposite film of tyrosinase-chitosan-carbon-coated nickel nanoparticles (CNi) had been constructed for the detection of catechol. The tyrosinase-chitosan-CNi bionanocomposite film was characterized with scanning electron microscopic (SEM) and electrochemical impedance spectroscopy (EIS). In pH 6.5 phosphate buffer solutions (PBS), the biosensor was applied to detect catechol with a broad linear range from 0.25 nM to 27 μM, the detection limit was brought down to 0.083 nM (S/N=3). The proposed biosensor demonstrated rapid response, as well as good reproducibility and stability. The chitosan-CNi film was propitious to the immobilization of tyrosinase and to the retention of its bioactivity to a large extent. Therefore, the film has potential applications in the immobilization of other enzyme-based biosensors.
authors.affiliation: Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.forename: Huayu
authors.initials: H
authors.lastname: Xiong
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhang
authors.forename: Shengfu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002396:Catechols / Q000032:analysis / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D057230:Limit of Detection; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D013997:Time Factors
chemical_list: D002396:Catechols; D002244:Carbon; D009532:Nickel; D048271:Chitosan; D014442:Monophenol Monooxygenase; C034221:catechol