title: Comparative pharmacokinetic study of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra in normal rats and the acute cholestasis hepatitis rats.
abstract: A comparative study was designed and conducted to compare the pharmacokinetic difference of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra to normal rats and the acute cholestasis hepatitis rats induced by alpha-naphthylisothiocyanate (ANIT). UPLC-ESI-MS/MS method was employed to determine the level of paeoniflorin and albiflorin in rat plasma using geniposide as the internal standard (IS). Unpaired Student's t-test was used for the statistical comparison. The investigation showed that there were significant differences between the normal rats and the acute cholestasis hepatitis rat groups in calculated parameters, such as AUC(0-t), AUC(0-∞), T(max) and CLz/F. The results indicated that acute liver injury in rats could alter the pharmacokinetics of drug. Since patients are the final users of the drug, it is essential to investigate the pharmacokinetics of the drug in disease status. Therefore, we used normal rats and the acute cholestasis hepatitis rats to study pharmacokinetics of Radix Paeoniae Rubra with the purpose of examining the influence of disease on the metabolic course.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing, China.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Jiang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jiabo
authors.initials: J
authors.lastname: Wang
authors.forename: Sisi
authors.initials: S
authors.lastname: Wei
authors.forename: Zhenman
authors.initials: Z
authors.lastname: Wei
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000208:Acute Disease; D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D001565:Benzoates / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D002779:Cholestasis / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D005960:Glucosides / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006505:Hepatitis / Q000188:drug therapy; D006801:Humans; D008297:Male; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013997:Time Factors; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin