title: Transmission of chronic wasting disease identifies a prion strain causing cachexia and heart infection in hamsters.
abstract: Chronic wasting disease (CWD) is an emerging prion disease of free-ranging and captive cervids in North America. In this study we established a rodent model for CWD in Syrian golden hamsters that resemble key features of the disease in cervids including cachexia and infection of cardiac muscle. Following one to three serial passages of CWD from white-tailed deer into transgenic mice expressing the hamster prion protein gene, CWD was subsequently passaged into Syrian golden hamsters. In one passage line there were preclinical changes in locomotor activity and a loss of body mass prior to onset of subtle neurological symptoms around 340 days. The clinical symptoms included a prominent wasting disease, similar to cachexia, with a prolonged duration. Other features of CWD in hamsters that were similar to cervid CWD included the brain distribution of the disease-specific isoform of the prion protein, PrP(Sc), prion infection of the central and peripheral neuroendocrine system, and PrP(Sc) deposition in cardiac muscle. There was also prominent PrP(Sc) deposition in the nasal mucosa on the edge of the olfactory sensory epithelium with the lumen of the nasal airway that could have implications for CWD shedding into nasal secretions and disease transmission. Since the mechanism of wasting disease in prion diseases is unknown this hamster CWD model could provide a means to investigate the physiological basis of cachexia, which we propose is due to a prion-induced endocrinopathy. This prion disease phenotype has not been described in hamsters and we designate it as the 'wasting' or WST strain of hamster CWD.
authors.affiliation: Department of Immunology and Infectious Diseases, Montana State University, Bozeman, Montana, United States of America. rbessen@montana.edu
authors.forename: Richard A
authors.initials: RA
authors.lastname: Bessen
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Robinson
authors.forename: Davis M
authors.initials: DM
authors.lastname: Seelig
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Watschke
authors.forename: Diana
authors.initials: D
authors.lastname: Lowe
authors.forename: Harold
authors.initials: H
authors.lastname: Shearin
authors.forename: Scott
authors.initials: S
authors.lastname: Martinka
authors.forename: Alex M
authors.initials: AM
authors.lastname: Babcock
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D001835:Body Weight; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002100:Cachexia / Q000150:complications* / Q000473:pathology; D006224:Cricetinae; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005247:Feeding Behavior; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009206:Myocardium / Q000473:pathology*; D009296:Nasal Cavity / Q000378:metabolism / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism / Q000473:pathology; D018620:PrPSc Proteins / Q000378:metabolism*; D013997:Time Factors; D034081:Wasting Disease, Chronic / Q000150:complications* / Q000635:transmission*
chemical_list: D018620:PrPSc Proteins