title: Profiling and characterization of volatile secretions from the European stink bug Graphosoma lineatum (Heteroptera: Pentatomidae) by two-dimensional gas chromatography/time-of-flight mass spectrometry.
abstract: An efficient method combining the headspace solid-phase microextraction (HS-SPME) sampling procedure and comprehensive two-dimensional gas-chromatography/time-of-flight mass spectrometry (GC×GC/TOF-MS) was established to study the volatile secretion components of stink bugs (Heteroptera: Pentatomidae). The combined power of this approach is illustrated by the identification of fifty-seven compounds in the secretion of a European stink-bug representative, Graphosoma lineatum. (E)-4-oxohex-2-enal and (E)-dec-2-enal were found to be the major components in the adult bug secretions followed by lower amounts of n-alkenal (C₅-C₁₂), n-alkenyl acetate (C₅-C₁₁), n-alkane (C₁₁-C₁₇) homologs, dienals and other compounds. More than thirty known compounds have been identified that had not been described before in G. lineatum adults. Of these compounds, (E)-4-oxohex-2-enal is of particular interest, since its isolation and identification, while calling some previous reports into question, clearly demonstrates a potential ability of our approach to yield artifact-free secretion profiles.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 16610 Prague 6, Czech Republic.
authors.forename: Miloslav
authors.initials: M
authors.lastname: Sanda
authors.forename: Petr
authors.initials: P
authors.lastname: Záček
authors.forename: Ludvík
authors.initials: L
authors.lastname: Streinz
authors.forename: Martin
authors.initials: M
authors.lastname: Dračínský
authors.forename: Bohumír
authors.initials: B
authors.lastname: Koutek
mesh_terms: D000447:Aldehydes / Q000032:analysis; D000475:Alkenes / Q000032:analysis; D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D020062:Heteroptera / Q000737:chemistry* / Q000502:physiology; D008297:Male; D009812:Odorants / Q000032:analysis; D052617:Solid Phase Microextraction / Q000379:methods; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: C476489:(2E)-decenal; C506591:4-oxo-(E)-2-hexenal; D000447:Aldehydes; D000475:Alkenes; D055549:Volatile Organic Compounds