title: Purification and characterization of maltooligosaccharide-forming α-amylase from moderately halophilic Marinobacter sp. EMB8.
abstract: Maltooligosaccharides especially maltotriose and maltotetraose producing amylases are highly desirable for application in bread making and other food industries. A maltotriose and maltotetraose producing amylase from moderately halophilic Marinobacter sp. EMB8 is described. Under optimized culture conditions, 48.0 IU/mL amylase was obtained. The enzyme was purified to homogeneity by ultrafiltration, DEAE cellulose and Sephadex G-75 column chromatography with 52% yield and 76-fold purification. It was a monomeric protein of 72 kDa. The amylase had many novel features viz. stability up to 20% NaCl, 80 °C temperature, pH 6.0-11.0 and in wide range of organic solvents at high concentrations. The enzyme efficiently hydrolyzed starch into maltooligosaccharides rich in maltotriose and maltotetraose. These novel properties make the Marinobacter sp. amylase a potentially useful enzyme.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Delhi, New Delhi, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Khare
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D053520:Marinobacter / Q000187:drug effects* / Q000201:enzymology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis*; D009930:Organic Chemicals / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology*; D012997:Solvents / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D000516:alpha-Amylases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D009930:Organic Chemicals; D012997:Solvents; C021705:maltooligosaccharides; D012965:Sodium Chloride; D000516:alpha-Amylases