title: Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain.
abstract: Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant produces two non-fluorescent PVDI precursors with a higher molecular mass than PVDI. In the present study, we describe the use of mass spectrometry to determine the structure of these PVDI precursors and show that they both contain a unformed chromophore like ferribactin, and either a myristic or myristoleic chain that must be removed before PVDI is secreted into the extracellular medium.
authors.affiliation: UMR 7242, Université de Strasbourg-CNRS, ESBS, Blvd. Sébastien Brant, F-67413 Illkirch, Strasbourg, France.
authors.forename: Mélissa
authors.initials: M
authors.lastname: Hannauer
authors.forename: Mathias
authors.initials: M
authors.lastname: Schäfer
authors.forename: Françoise
authors.initials: F
authors.lastname: Hoegy
authors.forename: Patrick
authors.initials: P
authors.lastname: Gizzi
authors.forename: Patrick
authors.initials: P
authors.lastname: Wehrung
authors.forename: Gaëtan L A
authors.initials: GL
authors.lastname: Mislin
authors.forename: Herbert
authors.initials: H
authors.lastname: Budzikiewicz
authors.forename: Isabelle J
authors.initials: IJ
authors.lastname: Schalk
mesh_terms: D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D005453:Fluorescence; D013058:Mass Spectrometry; D015394:Molecular Structure; D008970:Molecular Weight; D009154:Mutation; D019814:Myristic Acid / Q000737:chemistry*; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D005229:Fatty Acids, Monounsaturated; D009842:Oligopeptides; D017262:Siderophores; D019814:Myristic Acid; C042453:pyoverdin; D010453:Peptide Synthases; C054211:9-tetradecenoic acid