title: Cloning, differential expression, and association analysis with fat traits of porcine IDH3γ gene.
abstract: Mitochondrial NAD⁺-dependent isocitrate dehydrogenase (IDH3) catalyzes the allosterically regulated rate-limiting step of the tricarboxylic acid cycle activated. In pigs, very little is known about this gene. Here, we cloned 1,346 bp full-length cDNA and 8,778 bp genomic sequence of porcine γ subunit of IDH3 (IDH3γ). IDH3γ contains 12 exons separated by 11 introns. Real-time PCR revealed that IDH3γ mRNA were upregulated in backfat of Large White compared with Meishan and F1 hybrids, and most abundant in small intestine via tissue distribution profile. A microsatellite ("GT" repeats) in second intron was found. The selected pigs were genotyped at this microsatellite. The IDH3γ genotypes showed a significant effect on backfat thickness at thorax-waist (P < 0.05), backfat thickness at sixth to seventh thorax (P < 0.01), and average backfat thickness (P < 0.05). This site seemed to be significantly dominant in action (P < 0.05 for backfat thickness at sixth to seventh thorax, backfat thickness at thorax-waist, and average backfat thickness), and allele B was associated with increase of thickness values of these traits. This locus is possibly considered as a marker for adipose deposition traits.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China. renzq@mail.hzau.edu.cn
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Ren
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
authors.forename: Changyan
authors.initials: C
authors.lastname: Deng
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000818:Animals; D050218:Body Fat Distribution; D003001:Cloning, Molecular; D005091:Exons; D015870:Gene Expression; D056726:Genetic Association Studies; D005838:Genotype; D007421:Intestine, Small / Q000201:enzymology*; D007438:Introns; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D018895:Microsatellite Repeats; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D010641:Phenotype; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine
chemical_list: D021122:Protein Subunits; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007521:Isocitrate Dehydrogenase