title: miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells.
abstract: In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1). Moreover, miR-375 was upregulated and correlated with ASCL1 in the cell lines generated from mouse SCLC-like tumors as well. Dual-luciferase assays further showed that ASCL1 activated the expression of miR-375 by binding to the three E-box elements in the miR-375 promoter. These results imply a role of ASCL1 in SCLC via the upregulation of miR-375.
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Jin
authors.forename: Hongbin
authors.initials: H
authors.lastname: Ji
authors.forename: Xiumin
authors.initials: X
authors.lastname: Yan
authors.forename: Xueliang
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D017931:DNA Primers / Q000235:genetics; D024721:E-Box Elements; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D035683:MicroRNAs; C065500:ASCL1 protein, human; C499317:Ascl1 protein, mouse; C540933:MIRN375 microRNA, human; C540349:Mirn375 microRNA, mouse