title: Copy number variation analysis of matched ovarian primary tumors and peritoneal metastasis.
abstract: Ovarian cancer is the most deadly gynecological cancer. The high rate of mortality is due to the large tumor burden with extensive metastatic lesion of the abdominal cavity. Despite initial chemosensitivity and improved surgical procedures, abdominal recurrence remains an issue and results in patients' poor prognosis. Transcriptomic and genetic studies have revealed significant genome pathologies in the primary tumors and yielded important information regarding carcinogenesis. There are, however, few studies on genetic alterations and their consequences in peritoneal metastatic tumors when compared to their matched ovarian primary tumors. We used high-density SNP arrays to investigate copy number variations in matched primary and metastatic ovarian cancer from 9 patients. Here we show that copy number variations acquired by ovarian tumors are significantly different between matched primary and metastatic tumors and these are likely due to different functional requirements. We show that these copy number variations clearly differentially affect specific pathways including the JAK/STAT and cytokine signaling pathways. While many have shown complex involvement of cytokines in the ovarian cancer environment we provide evidence that ovarian tumors have specific copy number variation differences in many of these genes.
authors.affiliation: Genomics Core, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar.
authors.forename: Joel A
authors.initials: JA
authors.lastname: Malek
authors.forename: Eliane
authors.initials: E
authors.lastname: Mery
authors.forename: Yasmin A
authors.initials: YA
authors.lastname: Mahmoud
authors.forename: Eman K
authors.initials: EK
authors.lastname: Al-Azwani
authors.forename: Laurence
authors.initials: L
authors.lastname: Roger
authors.forename: Ruby
authors.initials: R
authors.lastname: Huang
authors.forename: Eva
authors.initials: E
authors.lastname: Jouve
authors.forename: Raphael
authors.initials: R
authors.lastname: Lis
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Thiery
authors.forename: Denis
authors.initials: D
authors.lastname: Querleu
authors.forename: Arash
authors.initials: A
authors.lastname: Rafii
mesh_terms: D000368:Aged; D019742:Chemokines, CC / Q000378:metabolism; D056915:DNA Copy Number Variations / Q000235:genetics*; D005260:Female; D052138:Genes, Neoplasm / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D053612:Janus Kinases / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000235:genetics* / Q000556:secondary*; D019707:Receptors, Chemokine / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D019742:Chemokines, CC; D019707:Receptors, Chemokine; D050791:STAT Transcription Factors; D053612:Janus Kinases