title: RAPD analysis of the genetic  diversity of mango (Mangifera indica) germplasm in Brazil.
abstract: We  evaluated genetic variability of mango (Mangifera indica) accessions  maintained in the Active Germplasm Bank of Embrapa Meio-Norte in Teresina,  Piauí, Brazil, using RAPDs. Among these accessions, 35 originated from  plantings in Brazil, six from the USA and one from India. Genomic DNA,  extracted from leaf material using a commercial purification kit, was subjected  to PCR with the primers A01, A09, G03, G10, N05, and M16. Fifty-five  polymorphic loci were identified, with mean of 9.16 ± 3.31 bands per primer and  100% polymorphism. Application of unweighted pair group method using arithmetic  average cluster analysis demonstrated five genotypic groups among the  accessions examined. The genotypes Rosa 41, Rosa 48 and Rosa 49 were highly  similar (94% similarity), whereas genotypes Sensation and Rosa 18 were the most  divergent (only 7% similarity). The mango accessions were found to have  considerable genetic variability, demonstrating the importance of analyzing  each genotype in a collection in order to efficiently maintain the germplasm  collection.
authors.affiliation: Programa em Genética e Melhoramento, Universidade Federal do Piauí, Campus Agrícola da Socopo, Teresina, PI, Brasil.
authors.forename: I G B
authors.initials: IG
authors.lastname: Souza
authors.forename: S E S
authors.initials: SE
authors.lastname: Valente
authors.forename: F B
authors.initials: FB
authors.lastname: Britto
authors.forename: V A B
authors.initials: VA
authors.lastname: de Souza
authors.forename: P S C
authors.initials: PS
authors.lastname: Lima
mesh_terms: D001938:Brazil; D001947:Breeding; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D030541:Databases, Genetic; D005819:Genetic Markers; D005838:Genotype; D055993:Germ Cells, Plant / Q000166:cytology / Q000378:metabolism*; D007194:India; D031022:Mangifera / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D014481:United States
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers