title: A rapid and sensitive HPLC-APCI-MS/MS method determination of fluticasone in human plasma: application for a bioequivalency study in nasal spray formulations.
abstract: A sensitive method for the determination of fluticasone in plasma was developed using high performance liquid chromatography with tandem mass spectrometric detection, whereas beclomethasone was used as internal standard. The analytes were extracted with a simple liquid-liquid extraction from the plasma samples and separated on an ACE C(18) 50 × 4.6 mm i.d.; 5 μm particle size column with a mobile phase consisting of acetonitrile - 0.01% formic acid (48:52, v/v) at a flow rate of 1 ml/min. Detection was achieved by an Applied Biosystems API 5000 mass spectrometer (LC-MS/MS) set at unit resolution in the multiple reaction monitoring mode. Atmospheric pressure chemical ionization (APCI) was used for ion production. The mean recovery for fluticasone propionate was 85%, with a lower limit of quantification set at 2 pg/mL. The validated analytical method was applied to a bioequivalence study of fluticasone propionate administered by nasal spray formulations in human volunteers.
authors.affiliation: Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, 74175-100 Goiânia, GO, Brazil. ricardobyrro@bol.com.br
authors.forename: Ricardo Martins Duarte
authors.initials: RM
authors.lastname: Byrro
authors.forename: Isabela Costa
authors.initials: IC
authors.lastname: César
authors.forename: Fabiana Fernandes
authors.initials: FF
authors.lastname: de Santana e Silva Cardoso
authors.forename: Iram Moreira
authors.initials: IM
authors.lastname: Mundim
authors.forename: Leonardo de Souza
authors.initials: Lde S
authors.lastname: Teixeira
authors.forename: Ricardo Rodrigues
authors.initials: RR
authors.lastname: Bonfim
authors.forename: Sandro Antônio
authors.initials: SA
authors.lastname: Gomes
authors.forename: Gerson Antônio
authors.initials: GA
authors.lastname: Pianetti
mesh_terms: D000328:Adult; D000730:Androstadienes / Q000097:blood* / Q000737:chemistry; D001274:Atmospheric Pressure; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D018592:Cross-Over Studies; D005260:Female; D000068298:Fluticasone; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods* / Q000592:standards; D059085:Nasal Sprays; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards; D013810:Therapeutic Equivalency; D013997:Time Factors
chemical_list: D000730:Androstadienes; D059085:Nasal Sprays; D000068298:Fluticasone