title: Comparison of CD63 Upregulation Induced by NSAIDs on Basophils and Monocytes in Patients with NSAID Hypersensitivity.
abstract: Background. An in vitro basophil activation test, based on the detection of CD63 upregulation induced by NSAIDs, has been described. Its clinical significance remains controversial. Objectives. In patients with a history of nonallergic NSAID hypersensitivity, stratified according to the severity of the symptoms, to assess with NSAIDs the predictive value of basophil (BAT) and monocyte (MAT) activation tests. Patients/Methods. Sixty patients who had NSAIDs-induced or exacerbated urticaria/angiooedema and 20 controls was included. After incubation with NSAIDs or acetaminophen, leukocytes were analysed for CD63 upregulation. Results. With aspirin, the sensitivity (37%) and specificity (90%) of BAT agree with already published results. In contrast, when patients had had cutaneous and visceral reactions, the frequency of positive BAT 14/22 (64%, P < 0.001) or MAT 10/22 (46%, P < 0.01) were increased. Conclusions. Positive tests were more frequent among patients having a severe hypersensitivity contrasting with the other patients who had results similar to controls.
authors.affiliation: Service d'Hématologie et d'Immunologie Biologique, Hôpital Tenon (AP-HP), Université Pierre et Marie Curie, 4 Rue de la Chine, 75020 Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Abuaf
authors.forename: H
authors.initials: H
authors.lastname: Rostane
authors.forename: J
authors.initials: J
authors.lastname: Barbara
authors.forename: C
authors.initials: C
authors.lastname: Toly-Ndour
authors.forename: H
authors.initials: H
authors.lastname: Gaouar
authors.forename: P
authors.initials: P
authors.lastname: Mathelier-Fusade
authors.forename: F
authors.initials: F
authors.lastname: Leynadier
authors.forename: C
authors.initials: C
authors.lastname: Francès
authors.forename: R
authors.initials: R
authors.lastname: Girot