title: Experimental limitations using reprogrammed cells for hematopoietic differentiation.
abstract: We review here our experiences with the in vitro reprogramming of somatic cells to induced pluripotent stem cells (iPSC) and subsequent in vitro development of hematopoietic cells from these iPSC and from embryonic stem cells (ESC). While, in principle, the in vitro reprogramming and subsequent differentiation can generate hematopoietic cell from any somatic cells, it is evident that many of the steps in this process need to be significantly improved before it can be applied to human cells and used in clinical settings of hematopoietic stem cell (HSC) transplantations.
authors.affiliation: Senior Research Group Lymphocyte Development, Max Planck Institute for Infection Biology, 10117 Berlin, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Seiler
authors.forename: Motokazu
authors.initials: M
authors.lastname: Tsuneto
authors.forename: Fritz
authors.initials: F
authors.lastname: Melchers
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D065150:Cellular Reprogramming; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D008954:Models, Biological