title: Sexual dimorphism in cardiac triacylglyceride dynamics in mice on long term caloric restriction.
abstract: Human studies indicate augmented myocardial lipid metabolism in females, and that sex and obesity interact to predict myocardial fatty acid oxidation and storage. Altered lipid dynamics precede cardiomyopathies, and many studies now address high fat diets. Conversely, caloric restriction (CR), is the most studied model for longevity and stress resistance, including protection against myocardial ischemia. However, no information exists on the effects of long-term caloric restriction (CR) on triacylglyceride (TAG) content and dynamics in the heart. This study explored the effects of CR, sex and age on TAG dynamics in mouse hearts. Male and female SVJ129 mice were fed either normal (ND) or CR diet for 3 or 10 months. In 5-month-old mice, CR similarly decreased cardiac TAG in males (ND: 25.5±4.5 nmol/mg protein; CR: 12.6±2.7, P<0.05) and females (ND: 30.1±4.4; CR: 13.7±1.2) (no significant differences in TAG content were seen between sexes). CR reduced the contribution of exogenous palmitate to oxidative metabolism in males and females, by 15% and 11% respectively, versus ND, without affecting cardiac workload. CR also induced a larger reduction in TAG turnover in male (68%) than female hearts (38%). Interestingly, in 5 month old male mice, CR reproduced the lower TAG turnover rates of middle-aged males (ND 13-month-old male=423±76 nmol/mg protein/min). Thus, long term CR reduces TAG pool dynamics. Despite reduced content, hearts of female mice subjected to CR retained a more dynamic TAG pool than males, while males respond with greater metabolic remodeling of cardiac lipid dynamics.
authors.affiliation: Program in Integrative Cardiac Metabolism, Center for Cardiovascular Research, University of Illinois at Chicago College of Medicine, Chicago, IL 60612, USA.
authors.forename: Natasha H
authors.initials: NH
authors.lastname: Banke
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.forename: Kayla M
authors.initials: KM
authors.lastname: Pound
authors.forename: Sunil
authors.initials: S
authors.lastname: Dhar
authors.forename: Heather
authors.initials: H
authors.lastname: Reinhardt
authors.forename: Mariana S
authors.initials: MS
authors.lastname: De Lorenzo
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Vatner
authors.forename: E Douglas
authors.initials: ED
authors.lastname: Lewandowski
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D001774:Blood Chemical Analysis; D031204:Caloric Restriction / Q000009:adverse effects*; D002247:Carbon Isotopes / Q000378:metabolism; D009202:Cardiomyopathies / Q000209:etiology; D005260:Female; D006439:Hemodynamics; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D009206:Myocardium / Q000378:metabolism*; D012737:Sex Factors; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D014280:Triglycerides; D000105:Acetyl Coenzyme A