title: Herbivore-induced plant volatiles can serve as host location cues for a generalist and a specialist egg parasitoid.
abstract: Herbivore-induced plant volatiles are important host finding cues for larval parasitoids, and similarly, insect oviposition might elicit the release of plant volatiles functioning as host finding cues for egg parasitoids. We hypothesized that egg parasitoids also might utilize HIPVs of emerging larvae to locate plants with host eggs. We, therefore, assessed the olfactory response of two egg parasitoids, a generalist, Trichogramma pretiosum (Tricogrammatidae), and a specialist, Telenomus remus (Scelionidae) to HIPVs. We used a Y-tube olfactometer to tests the wasps' responses to volatiles released by young maize plants that were treated with regurgitant from caterpillars of the moth Spodoptera frugiperda (Noctuidae) or were directly attacked by the caterpillars. The results show that the generalist egg parasitoid Tr. pretiosum is innately attracted by volatiles from freshly-damaged plants 0-1 and 2-3 h after regurgitant treatment. During this interval, the volatile blend consisted of green leaf volatiles (GLVs) and a blend of aromatic compounds, mono- and homoterpenes, respectively. Behavioral assays with synthetic GLVs confirmed their attractiveness to Tr. pretiosum. The generalist learned the more complex volatile blends released 6-7 h after induction, which consisted mainly of sesquiterpenes. The specialist T. remus on the other hand was attracted only to volatiles emitted from fresh and old damage after associating these volatiles with oviposition. Taken together, these results strengthen the emerging pattern that egg and larval parasitoids behave in a similar way in that generalists can respond innately to HIPVs, while specialists seems to rely more on associative learning.
authors.affiliation: Department of Entomology and Acarology, Laboratory of Chemical Ecology and Insect Behavior, University of São Paulo, Escola Superior de Agricultura Luiz de Queiroz, Av. Pádua Dias, 11, CP 09, 13418-900, Piracicaba, SP, Brazil.
authors.forename: M F G V
authors.initials: MF
authors.lastname: Peñaflor
authors.forename: M
authors.initials: M
authors.lastname: Erb
authors.forename: L A
authors.initials: LA
authors.lastname: Miranda
authors.forename: A G
authors.initials: AG
authors.lastname: Werneburg
authors.forename: J M S
authors.initials: JM
authors.lastname: Bento
mesh_terms: D000818:Animals; D001245:Association Learning; D002633:Chemotaxis; D003463:Cues; D005247:Feeding Behavior; D008401:Gas Chromatography-Mass Spectrometry; D007323:Instinct; D007814:Larva / Q000469:parasitology / Q000502:physiology; D009812:Odorants; D010058:Oviposition; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D012903:Smell; D013045:Species Specificity; D018411:Spodoptera / Q000469:parasitology* / Q000502:physiology; D055549:Volatile Organic Compounds / Q000032:analysis*; D014863:Wasps / Q000502:physiology*; D003313:Zea mays / Q000737:chemistry* / Q000378:metabolism
chemical_list: D055549:Volatile Organic Compounds