title: Phytochemical analysis, cytotoxic activity and constituents-activity relationships of the leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae).
abstract: The leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae) have been refluxed successively with chloroform and alcohol to get chloroform extract and alcoholic extract. Both the extracts have been assayed for cytotoxicity against human colorectal tumour cells. The chloroform extract exhibited significant cytotoxicity with IC(50) 31 µg mL(-1) (p < 0.01). However, ethanol extract was found to be much less cytotoxic with IC(50) > 200 µg mL(-1). The chloroform extract has been further proceeded for chemical analysis by GC-TOFMS and 178 components were identified including acids, amines, amides, aldehydes, alcohols, esters, benzene derivatives, bicyclic compounds, terpenes, hydrocarbons, naphthalene derivatives, furan derivatives, azulenes, etc. Nine components representing 51.73% of the total chloroform extract were detected as major components. Caryophyllene (14.41%) and Eicosanoic acid ethyl ester (12.17%) are the most prominent components of the chloroform extract. β-Caryophyllene (14.41%) as most abundant compound supports potent cytotoxicity as shown by chloroform extract.
authors.affiliation: School of Industrial Technology, Universiti Sains Malaysia, Minden - 11800, Pulau Pinang, Malaysia. raza2005communications@gmail.com
authors.forename: Raza Murad
authors.initials: RM
authors.lastname: Ghalib
authors.forename: Rokiah
authors.initials: R
authors.lastname: Hashim
authors.forename: Othman
authors.initials: O
authors.lastname: Sulaiman
authors.forename: Sayed Hasan
authors.initials: SH
authors.lastname: Mehdi
authors.forename: Zurida
authors.initials: Z
authors.lastname: Anis
authors.forename: Syed Ziaur
authors.initials: SZ
authors.lastname: Rahman
authors.forename: B M Khadeer
authors.initials: BM
authors.lastname: Ahamed
authors.forename: Amin Malik Shah
authors.initials: AM
authors.lastname: Abdul Majid
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D019770:Lauraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts