title: Separation of two constituents from purple sweet potato by combination of silica gel column and high-speed counter-current chromatography.
abstract: It is known that the choice of solvent system for high speed counter-current chromatography separation is of utmost importance. In this study, a simple and rapid thin layer chromatograph coupling with fluorometric (TLC-F) method has been used to determine the partition coefficient of target compounds in HSCCC solvent system. Two components, 6,7-dimethoxycoumarin and 5-hydroxymethyl-2-furfural were successfully separated from purple sweet potato extracts by successive sample injection for the first time, using n-hexane-ethyl acetate-methanol-water (1:2:1:1, v/v/v/v) as the solvent system. Additionally, statistical analysis showed that there was no significant difference in partition coefficient obtained by the TLC-F method and by HPLC, which demonstrated the usefulness of TLC-F method.
authors.affiliation: School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Kai
authors.initials: K
authors.lastname: He
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
authors.forename: Hongying
authors.initials: H
authors.lastname: Chen
authors.forename: Lujiang
authors.initials: L
authors.lastname: Yuan
authors.forename: Yafei
authors.initials: Y
authors.lastname: Deng
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaoduo
authors.initials: X
authors.lastname: Li
mesh_terms: D000085:Acetates; D002855:Chromatography, Thin Layer / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification; D003377:Countercurrent Distribution / Q000379:methods*; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000302:isolation & purification; D006586:Hexanes; D027723:Ipomoea batatas / Q000737:chemistry*; D000432:Methanol; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D058428:Silica Gel; D014867:Water
chemical_list: D000085:Acetates; D003374:Coumarins; D006586:Hexanes; D010936:Plant Extracts; D014867:Water; C026385:n-hexane; D058428:Silica Gel; C008046:5-hydroxymethylfurfural; C007650:ethyl acetate; D005662:Furaldehyde; C018145:scoparone; D000432:Methanol