title: Find and replace: editing human genome in pluripotent stem cells.
abstract: Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine. Recently a number of independent genome-editing techniques were developed, including plasmid, bacterial artificial chromosome, adeno-associated virus vector, zinc finger nuclease, transcription activator-like effecter nuclease, and helper-dependent adenoviral vector. Gene editing has been successfully employed in different aspects of stem cell research such as gene correction, mutation knock-in, and establishment of reporter cell lines (Raya et al., 2009; Howden et al., 2011; Li et al., 2011; Liu et al., 2011b; Papapetrou et al., 2011; Sebastiano et al., 2011; Soldner et al., 2011; Zou et al., 2011a). These techniques combined with the utility of hPSCs will significantly influence the area of regenerative medicine.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Huize
authors.initials: H
authors.lastname: Pan
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zhang
authors.forename: Weizhou
authors.initials: W
authors.lastname: Zhang
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics; D000229:Dependovirus / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D015894:Genome, Human; D006801:Humans; D016254:Mutagenesis, Insertional; D009154:Mutation; D010957:Plasmids; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D003851:Deoxyribonucleases