title: Overpressured layer chromatography: from the pressurized ultramicro chamber to BioArena system.
abstract: The pressurized ultramicro (UM) chamber as a closed adsorbent layer chamber enables the use of a special chromatoplate and a pump to increase and optimize the mobile phase flow velocity through an optional development distance in an adsorbent layer. This chamber is the basic instrument of overpressured-layer chromatography (OPLC), which is a separation technique that combines the advantages of conventional TLC/HPTLC with those of HPLC. The versions of OPLC instrument, the character and achievement of off-line and on-line OPLC systems in analytical and preparative use are described. The development of BioArena as a complex bioautographic system means an exploitation of the unique advantages of planar-layer system for detection, isolation and identification of new antimicrobials, antineoplastics, biopesticides and other biologically active substances as well as for studying fundamental biochemical reactions and mechanisms.
authors.affiliation: Plant Protection Institute, Hungarian Academy of Sciences, Herman O. Str. 15, POB 102, Budapest H-1525, Hungary. etyih@nki.hu
authors.forename: Erno
authors.initials: E
authors.lastname: Tyihák
authors.forename: Emil
authors.initials: E
authors.lastname: Mincsovics
authors.forename: Agnes M
authors.initials: AM
authors.lastname: Móricz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D004364:Pharmaceutical Preparations / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D011312:Pressure
chemical_list: D004364:Pharmaceutical Preparations; D010936:Plant Extracts