title: Evaluation of antiangiogenic and antoxidant properties of Parkia speciosa Hassk extracts.
abstract: Parkia speciosa Hassk is a traditional medicinal plant with strong antioxidant and hypoglycemic properties. This study aims to investigate the total phenolic content, antioxidant, cytotoxic and antiangiogenic effect of eight extracts from P. speciosa empty pods. The extracts were found to contain high levels of total phenols and demonstrated strong antioxidant effect in DPPH scavenging test. In rat aortic rings, P. speciosa extracts significantly inhibited the microvessel outgrowth from aortic tissue explants by more than 50%. The antiangiogenic activity was further confirmed by tube formation on matrigel matrix involving human endothelial cells. Cytotoxic effect was evaluated by XTT test on endothelial cells as a model of angiogenesis versus a panel of human cancer and normal cell lines. Basically the extracts did not show acute cytotoxicity. Morphology examination of endothelial cells indicated induction of autophagy characterized by formation of plenty of cytoplasmic vacuoles. The extracts were found to work by decreasing expression of vascular endothelial growth factor in endothelial cells.
authors.affiliation: School of Pharmaceutical Sciences, University Science Malaysia, Minden, Pulau Pinang, Malaysia.
authors.forename: Abdalrahim F A
authors.initials: AF
authors.lastname: Aisha
authors.forename: Khalid M
authors.initials: KM
authors.lastname: Abu-Salah
authors.forename: Salman A
authors.initials: SA
authors.lastname: Alrokayan
authors.forename: Zhari
authors.initials: Z
authors.lastname: Ismail
authors.forename: Amin Malik Shah
authors.initials: AM
authors.lastname: Abdulmajid
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008297:Male; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020533:Angiogenesis Inhibitors; D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A