title: Viability of mesenchymal stem cells during electrospinning.
abstract: Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue staining. Fiber diameter was observed by scanning electron microscopy and the presence of cells within the scaffolds was analyzed by confocal laser scanning microscopy. After electrospinning, the viability of mesenchymal stem cells was reduced from 88 to 19.6% and the viability of mononuclear cells from 99 to 8.38%. The loss of viability was possibly due to the high viscosity of the polymer solution, which reduced the access to nutrients associated with electric and mechanical stress during electrospinning. These results suggest that the incorporation of cells during fiber formation by electrospinning is a viable process that needs more investigation in order to find ways to protect cells from damage.
authors.affiliation: Laboratório de Hematologia e Células-tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
authors.forename: G
authors.initials: G
authors.lastname: Zanatta
authors.forename: D
authors.initials: D
authors.lastname: Steffens
authors.forename: D I
authors.initials: DI
authors.lastname: Braghirolli
authors.forename: R A
authors.initials: RA
authors.lastname: Fernandes
authors.forename: C A
authors.initials: CA
authors.lastname: Netto
authors.forename: P
authors.initials: P
authors.lastname: Pranke
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D002470:Cell Survival; D004563:Electrochemistry / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D036103:Nanotechnology / Q000379:methods; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D054457:Tissue Scaffolds; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D001672:Biocompatible Materials; D011142:Polyvinyl Alcohol