title: Genetic characterization of Tribeč virus and Kemerovo virus, two tick-transmitted human-pathogenic Orbiviruses.
abstract: We determined the complete genome sequences of Tribeč virus (TRBV) and Kemerovo virus (KEMV), two tick-transmitted Orbiviruses that can cause diseases of the central nervous system and that are currently classified into the Great Island virus serogroup. VP2 proteins of TRBV and KEMV show very low sequence similarity to the homologous VP4 protein of tick-transmitted Great Island virus (GIV). The new sequence data support previous serological classification of these Orbiviruses into the Kemerovo serogroup, which is different from the Great Island virus serogroup. Genome segment 9 of TRBV and KEMV encodes several overlapping ORF's in the +1 reading frame relative to VP6(Hel). A co-phylogenetic analysis indicates a host switch from insect-borne Orbiviruses toward Ixodes species, which is in disagreement with previously published data.
authors.affiliation: Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany. meik.dilcher@medizin.uni-goettingen.de
authors.forename: Meik
authors.initials: M
authors.lastname: Dilcher
authors.forename: Lekbira
authors.initials: L
authors.lastname: Hasib
authors.forename: Marcus
authors.initials: M
authors.lastname: Lechner
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wieseke
authors.forename: Martin
authors.initials: M
authors.lastname: Middendorf
authors.forename: Manja
authors.initials: M
authors.lastname: Marz
authors.forename: Andrea
authors.initials: A
authors.lastname: Koch
authors.forename: Martin
authors.initials: M
authors.lastname: Spiegel
authors.forename: Gerhard
authors.initials: G
authors.lastname: Dobler
authors.forename: Frank T
authors.initials: FT
authors.lastname: Hufert
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D001483:Base Sequence; D002460:Cell Line; D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D018163:Orbivirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012088:Reoviridae Infections / Q000821:virology*; D016415:Sequence Alignment; D013987:Ticks / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins