title: Physiological and behavioral responses to an electrical stimulus in Mozambique tilapia (Oreochromis mossambicus).
abstract: Consumer awareness of the need to improve fish welfare is increasing. Electrostunning is a clean and potentially efficient procedure more and more used to provoke loss of consciousness prior to killing or slaughtering (reviewed by Van de Vis et al. in Aquac Res 34:211-220, 2003). Little is known how (powerful) electrical stimuli, which do not stun immediately, are perceived by fish. We investigated responses of hand-held Mozambique tilapia (Oreochromis mossambicus) to a standardized electric shock applied to the tailfin. The handling with the resulting unavoidable acute stress response was carefully controlled for. Fish responses were analyzed up to 24 h following the shock. Electric shock resulted in slightly higher levels in plasma cortisol, lactate, ionic levels, and osmolality, than handling alone. Plasma glucose had significantly increased 6 h after shock compared to handling, indicative of enhanced adrenergic activity. Mucus release from the gills, branchial Na⁺/K⁺ ATPase activity, and chloride cell migration and proliferation, parameters that will change with strong adrenergic activation, were not affected. Decreased swimming activity and delay in resumption of chafing behavior indicated a stronger and differential response toward the electric shock. Responses to handling lasted shorter compared to those to an electric shock. The differential and stronger responses to the electric shock suggest that fish perceived the shock potentially as painful.
authors.affiliation: Department of Animal Physiology, Faculty of Science, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, Box 30, 6525 AJ, Nijmegen, The Netherlands.|Department of Aquaculture, IMARES Wageningen UR, Institute for Marine Resources and Ecosystem Studies, P.O. Box 77, 4400 AB, Yerseke, The Netherlands.
authors.forename: Jonathan A C
authors.initials: JAC
authors.lastname: Roques
authors.affiliation: Department of Aquaculture, IMARES Wageningen UR, Institute for Marine Resources and Ecosystem Studies, P.O. Box 77, 4400 AB, Yerseke, The Netherlands.
authors.forename: Wout
authors.initials: W
authors.lastname: Abbink
authors.affiliation: Department of Animal Physiology, Faculty of Science, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, Box 30, 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Gaétan
authors.initials: G
authors.lastname: Chereau
authors.affiliation: Department of Animal Physiology, Faculty of Science, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, Box 30, 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Fourneyron
authors.affiliation: Department of Animal Physiology, Faculty of Science, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, Box 30, 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Tom
authors.initials: T
authors.lastname: Spanings
authors.affiliation: Department of Aquaculture, IMARES Wageningen UR, Institute for Marine Resources and Ecosystem Studies, P.O. Box 77, 4400 AB, Yerseke, The Netherlands.
authors.forename: Dirk
authors.initials: D
authors.lastname: Burggraaf
authors.affiliation: Department of Animals in Science and Society, Division of Behavioural Neuroscience, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.166, 3508 TD, Utrecht, The Netherlands.
authors.forename: Ruud
authors.initials: R
authors.lastname: van de Bos
authors.affiliation: Department of Aquaculture, IMARES Wageningen UR, Institute for Marine Resources and Ecosystem Studies, P.O. Box 77, 4400 AB, Yerseke, The Netherlands.
authors.forename: Hans
authors.initials: H
authors.lastname: van de Vis
authors.affiliation: Department of Animal Physiology, Faculty of Science, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, Box 30, 6525 AJ, Nijmegen, The Netherlands. g.flik@science.ru.nl.
authors.forename: Gert
authors.initials: G
authors.lastname: Flik
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D004558:Electric Stimulation; D006234:Handling, Psychological; D006854:Hydrocortisone / Q000097:blood; D007477:Ions / Q000378:metabolism; D059225:Nociception; D013312:Stress, Physiological; D013550:Swimming; D017210:Tilapia / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D007477:Ions; D006854:Hydrocortisone