title: Electrospun polyacrylonitrile nanofibrous membranes for chitosanase immobilization and its application in selective production of chitooligosaccharides.
abstract: Polyacrylonitrile nanofibrous membranes (PANNFM) were prepared by electrospinning from 10 wt.% of PAN solution and its surface was modified by amidination reaction. A new chitosan degrading enzyme from Aspergillus sp. was covalently immobilized on PANNFM. Immobilization efficiency of 80% was achieved by activating PANNFM surface for 30 min followed by 2 h treatment with enzyme solution. The optimum temperature and pH for immobilized enzyme were 50°C and 5.8, respectively. The immobilized chitosanase retained >70% activity after ten repeated batch reaction and could be stored up to 60 days at 4°C with minor loss in activity. Chitosan hydrolysis using different substrates were studied using immobilized chitosanase in batch conditions. Continuous selective production of chitooligosaccharides (dimer to hexamer) by changing the temperature was achieved by PANNFM-chitosanase.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi, India. sinha738@gmail.com
authors.forename: Sujata
authors.initials: S
authors.lastname: Sinha
authors.forename: Sanjay R
authors.initials: SR
authors.lastname: Dhakate
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kumar
authors.forename: R B
authors.initials: RB
authors.lastname: Mathur
authors.forename: Pushplata
authors.initials: P
authors.lastname: Tripathi
authors.forename: Subhash
authors.initials: S
authors.lastname: Chand
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D048271:Chitosan / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005944:Glucosamine / Q000032:analysis; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry*; D009844:Oligosaccharides / Q000378:metabolism*; D059027:Recycling; D013696:Temperature; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009844:Oligosaccharides; C010504:polyacrylonitrile; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase; D005944:Glucosamine