title: Annexin 1 protects against apoptosis induced by serum deprivation in transformed rat retinal ganglion cells.
abstract: To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by western blot and RT-PCR. Effects of varying concentrations of the Annexin 1 peptide fragment, Ac2-26, on the survival of the RGC-5 cells was determined, and apoptotic cells were quantified by flow cytometry. Immunoblot and RT-PCR analysis was preformed to identify caspase 3, bax and bcl-2 in RGC extracts. Annexin 1 was localized in the cytoplasm of RGC-5 cells and the expression of Annexin 1, caspase 3 and bax was upregulated in serum-deprived RGC-5 cells. Ac2-26 attenuated the apoptosis resulting from serum deprivation of RGC-5 in a concentration-dependent manner, decreased caspase 3 and bax levels and produced an increase of bcl-2 in cell lysates. Annexin 1, in specific the peptide fragment Ac2-26, may play an important role in decreasing apoptosis in serum-deprived RGC-5 cells.
authors.affiliation: Department of Ophthalmology, The 2nd Affiliated Hospital, Harbin Medical University, 246 Xuefu Road, Harbin, 150086, Heilongjiang, People's Republic of China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.forename: Zhengbo
authors.initials: Z
authors.lastname: Shao
authors.forename: Yuanhang
authors.initials: Y
authors.lastname: Yang
authors.forename: Donglai
authors.initials: D
authors.lastname: Wu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhou
authors.forename: Huiping
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002461:Cell Line, Transformed; D016895:Culture Media, Serum-Free; D019610:Cytoprotection / Q000187:drug effects*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D011419:Propidium / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D017305:Annexin A1; D016895:Culture Media, Serum-Free; D018696:Neuroprotective Agents; D010455:Peptides; D012333:RNA, Messenger; C096153:annexin A1 peptide (2-26); D051028:bcl-2-Associated X Protein; D011419:Propidium; D053148:Caspase 3; D016650:Fluorescein-5-isothiocyanate