title: Effects of varying dilutions, pH, temperature and cations on spermatozoa motility in fish Larimichthys polyactis.
abstract: The objectives of this present study were to assess the effects of varying dilutions, pH, temperature and cations on spermatozoa motile parameters (SMPs) in fish Larimichthys polyactis. Optimal SMPs were observed when emen was diluted in artificial seawater (ASW) at a ratio of 1 to 100, with temperature of 10 degreesC and pH 8.0. The spermatozoa of L. polyactis were immotile in distilled water and motile in solution containing different cations. Maximum SMPs were obtained in each solution containing 0.4 mol NaCI, 0.4 mol KCI, 0.2 mol CaCI2 and 0.2 mol MgCl2. This study provides baseline knowledge of L. polyactis spermatozoa sensitivity of pH, temperature and cationic effects.
authors.affiliation: Department of Aquaculture, Pukyong National University, Busan 608-737, Korea.
authors.forename: Hoang
authors.initials: H
authors.lastname: Le Minh
authors.forename: Han Kyu
authors.initials: HK
authors.lastname: Lim
authors.forename: Byung Hwa
authors.initials: BH
authors.lastname: Min
authors.forename: Mi Seon
authors.initials: MS
authors.lastname: Park
authors.forename: Maeng-Hyun
authors.initials: MH
authors.lastname: Son
authors.forename: Jung Uie
authors.initials: JU
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D002412:Cations; D003470:Culture Media / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D010473:Perciformes / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D002412:Cations; D003470:Culture Media