title: Identification and expression profile of a new cytochrome P450 isoform (CYP414A1) in the hepatopancreas of Venerupis (Ruditapes) philippinarum exposed to benzo[a]pyrene, cadmium and copper.
abstract: With the objective to identify promising molecular biomarkers for marine pollution monitoring, a new cytochrome P450 gene was identified from Venerupis (Ruditapes) philippinarum and classified as a member of a new subfamily, CYP414A1. Phylogenetic analysis showed that CYP414A1 was closely related to members of the CYP2 family. CYP414A1 mRNA expression was significantly induced by 50 μg/L B[a]P at 96 h, while no significant change was found in 5 μg/L B[a]P-exposed samples. For heavy metals exposure, the expression of CYP414A1 was significantly up-regulated by Cd but sharply depressed by Cu exposure. These results suggested that CYP414A1 responded to various xenobiotics stresses, and could be used as a candidate biomarker of heavy metals and B[a]P.
authors.affiliation: Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Linbao
authors.initials: L
authors.lastname: Zhang
authors.forename: Junli
authors.initials: J
authors.lastname: Gan
authors.forename: Changliang
authors.initials: C
authors.lastname: Ke
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Liping
authors.initials: L
authors.lastname: You
authors.forename: Junbao
authors.initials: J
authors.lastname: Yu
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D049872:Bivalvia / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology; D002104:Cadmium / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D043143:Hepatopancreas / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D002104:Cadmium; D001564:Benzo(a)pyrene; D003300:Copper; D003577:Cytochrome P-450 Enzyme System