title: Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
abstract: BACKGROUND
  Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in BPH behavior. However, the olfaction related proteins have not been characterized in this insect.
  
  
  RESULTS
  Full length cDNA of three OBPs were obtained and distinct expression profiles were revealed regarding to tissue, developmental stage, wing form and gender for the first time for the species. The results provide important clues in functional differentiation of these genes. Binding assays with 41 compounds demonstrated that NlugOBP3 had markedly higher binding ability and wider binding spectrum than the other two OBPs. Terpenes and Ketones displayed higher binding while Alkanes showed no binding to the three OBPs. Focused on NlugOBP3, RNA interference experiments showed that NlugOBP3 not only involved in nymph olfaction on rice seedlings, but also had non-olfactory functions, as it was closely related to nymph survival.
  
  
  CONCLUSIONS
  NlugOBP3 plays important roles in both olfaction and survival of BPH. It may serve as a potential target for developing behavioral disruptant and/or lethal agent in N. lugens.
authors.affiliation: Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Nai-Yong
authors.initials: NY
authors.lastname: Liu
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.forename: Shuang-Lin
authors.initials: SL
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005247:Feeding Behavior; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006430:Hemiptera / Q000235:genetics* / Q000254:growth & development; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D014835:Volatilization; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D008024:Ligands; D012333:RNA, Messenger; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C045648:odorant-binding protein