title: Hepatoprotective glycosides from Leonurus japonicus Houtt.
abstract: Two new phenylethanoid glycosides 1 and 2 named leonoside E and leonoside F, and one new sesquiterpene glycoside (3) identified as 7α (H)-eudesmane-4,11 (12)-diene-3-one-2β-hydroxy-13-β-d-glucopyranoside, together with seven known glycosides (4-10), were isolated from the aerial part of Leonurus japonicus Houtt. Their structures were elucidated on the basis of spectroscopic data and chemical evidence. When tested in in vitro assays, compounds 1, 2, 4, and 6 exhibited potent hepatoprotective activity against d-galactosamine-induced toxicity in HL-7702 cells at concentration of 1×10(-5) M.
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Yixiu
authors.initials: Y
authors.lastname: Li
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Ziming
authors.initials: Z
authors.lastname: Feng
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jianshuang
authors.initials: J
authors.lastname: Jiang
authors.forename: Peicheng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D002240:Carbohydrate Sequence; D002460:Cell Line; D019610:Cytoprotection; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D031332:Leonurus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry; D045787:Sesquiterpenes, Eudesmane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014312:Trisaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C572242:2-(3-methoxy-4-hydroxyphenyl)ethanol-O-arabinopyranosyl-(1-2)-rhamnopyranosyl-(1-3)-glucopyranoside; C572243:2-(3-methoxyl-4-hydroxyphenyl)ethanol-O-rhamnopyranosyl-(1-3) (glucopyranosyl-(1-6))-glucopyranoside; D005960:Glucosides; D006027:Glycosides; D010936:Plant Extracts; D012717:Sesquiterpenes; D045787:Sesquiterpenes, Eudesmane; D014312:Trisaccharides; C015872:eudesmane; D006139:Guaiacol