title: Exploring N-imidazolyl-O-carboxymethyl chitosan for high performance gene delivery.
abstract: Chitosan shows good biocompatibility and biodegradability, but the poor water solubility and low transfection efficiency hinder its applications as a gene delivery vector. We here report the detailed synthesis and characterization of a novel ampholytical chitosan derivative, N-imidazolyl-O-carboxymethyl chitosan (IOCMCS), used for high performance gene delivery. After chemical modification, the solubility of the resulting polymer is enhanced, and the polymer is soluble in a wide pH range (4-10). Gel electrophoresis study reveals the strong binding ability between plasmid DNA and the IOCMCS. Moreover, the IOCMCS does not induce remarkable cytotoxicity against human embryonic kidney (HEK293T) cells. The cell transfection results with HEK293T cells using the IOCMCS as gene delivery vector demonstrate the high transfection efficiency, which is dependent on the degree of imidazolyl substitution. Therefore, the IOCMCS is a promising candidate as the DNA delivery vector in gene therapy due to its high solubility, high gene binding capability, low cytotoxicity, and high gene transfection efficiency.
authors.affiliation: School of Chemical Engineering, The University of Adelaide, Adelaide SA5005, Australia.
authors.forename: Bingyang
authors.initials: B
authors.lastname: Shi
authors.forename: Zheyu
authors.initials: Z
authors.lastname: Shen
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
authors.forename: Jingxiu
authors.initials: J
authors.lastname: Bi
authors.forename: Sheng
authors.initials: S
authors.lastname: Dai
mesh_terms: D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing / Q000379:methods; D010957:Plasmids / Q000737:chemistry / Q000494:pharmacology; D012995:Solubility; D014162:Transfection
chemical_list: D004247:DNA; D048271:Chitosan