title: Generation of transgenic chicks  using an oviduct-specific expression system.
abstract: We successfully replaced the ovalbumin gene of a magnum region in chickens with a human plasminogen activator. We constructed pL-eGFP, pL-tPAGFP and pL-2.8OVtPAGFP vectors and cultured 293FT chicken embryo fibroblasts, chicken primordial germ cells, Hela C127 cells, and oviduct epithelial cells. All vectors were expressed in the transfected cells, except pL-2.8OVtPAGFP vector, which was only expressed in oviduct epithelial cells. A lentivirus with pL-2.8OVtPAGFP was injected in fertilized eggs; 11 chicks hatched in the G₀ generation, four of them carried the tPAGFP. Two cockerels from the G₀ generation were crossed with four wild-type hens. Three chicks in G₁ carried the tPAGFP. We concluded that by using an oviduct-specific vector for transfection, human recombinant plasminogen activator protein can be expressed in the oviducts of laying hens. This character is inherited and can be reproduced with a need for repeated transfection.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. hkaleri@hotmail.com
authors.forename: H A
authors.initials: HA
authors.lastname: Kaleri
authors.forename: S Y
authors.initials: SY
authors.lastname: Xu
authors.forename: H L
authors.initials: HL
authors.lastname: Lin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry*; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D010047:Ovalbumin / Q000235:genetics / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism; D010960:Plasminogen Activators / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D015053:Zygote
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D010047:Ovalbumin; D010960:Plasminogen Activators