title: Characterization of human B cells in umbilical cord blood-transplanted NOD/SCID mice.
abstract: Humanized mice are crucially important for preclinical studies. However, the development and potential function of human B cells in chimeras remain unclear. Here, we describe the study of human B cells in NOD/LtSzPrkdcscid/J (NOD/SCID) mice. In this study, we transplanted 1.0×10(5) human CD34(+) cells from umbilical cord blood (UCB) into NOD/SCID mice after pretreatment with anti-asialo GM1 antiserum and sublethal irradiation. Human CD45(+) cells were detected in the peripheral blood of the recipient mice from 6 weeks after transplantation. CD19(+) B cells accounted for the greater part of the CD45(+) cells in the human UCB-chimeric mice, but their maturational stages differed in different organs. Most of the bone marrow (BM) CD19(+) cells were immature IgM(-)IgD(-)CD24(hi)CD38(hi) B cells, whereas the mature CD5(+)IgM(+)IgD(+)CD24(int)CD38(int)CD19(+) B cells were predominantly present in the spleen and peripheral blood. Human immunoglobulin (Ig) M was detected in mouse plasma. The human B cells also secreted human interleukin-10 after stimulation with LPS in vitro. These results show that human CD34(+) cells can differentiate into human B cells in NOD/SCID mice, with development and functions that are similar to those of B cell subsets in humans. The transplantation of human CD34(+) cells into NOD/SCID mice may provide a useful tool to study the development and function of human B cells.
authors.affiliation: Institute of Immunology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
authors.forename: Xuefu
authors.initials: X
authors.lastname: Wang
authors.forename: Ziping
authors.initials: Z
authors.lastname: Qi
authors.forename: Haiming
authors.initials: H
authors.lastname: Wei
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tian
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D036101:Cord Blood Stem Cell Transplantation; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D019172:Transplantation Conditioning; D014183:Transplantation, Heterologous
chemical_list: D015703:Antigens, CD; C508609:IL10 protein, human; D016753:Interleukin-10