title: Multifocus confocal Raman microspectroscopy for rapid single-particle analysis.
abstract: We have developed a multifocus confocal Raman microspectroscopy system that allows simultaneous analyses of ≈ 80 individual biological or airborne microparticles based on a precise image-guided technique. Multiple individual particles adhered in random positions on a coverslip were illuminated by a multifocus excitation pattern formed by rapidly steering a single laser beam with a pair of galvo-mirrors, and their Raman scatterings were synchronously projected with another galvo-mirror to different rows of a CCD chip for parallel spectroscopic analyses. We show that this technique can be used to rapidly identify single airborne particles or bacteria collected on a slide and to monitor germination dynamics of multiple bacterial spores in real-time.
authors.forename: Lingbo
authors.initials: L
authors.lastname: Kong
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Peter
authors.initials: P
authors.lastname: Setlow
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000302:isolation & purification; D001407:Bacillus / Q000737:chemistry / Q000302:isolation & purification; D004867:Equipment Design; D010316:Particle Size; D012815:Signal Processing, Computer-Assisted; D059010:Single-Cell Analysis / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000393:Air Pollutants