title: Determination of ABO genotypes by real-time PCR using allele-specific primers.
abstract: ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.
authors.affiliation: Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Muro
authors.forename: Junko
authors.initials: J
authors.lastname: Fujihara
authors.forename: Shinji
authors.initials: S
authors.lastname: Imamura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Kaori
authors.initials: K
authors.lastname: Kimura-Kataoka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Toga
authors.forename: Reiko
authors.initials: R
authors.lastname: Iida
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D017931:DNA Primers; D053803:Forensic Genetics / Q000379:methods; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000017:ABO Blood-Group System; D017931:DNA Primers