title: Differential gene expression in different types of Hevea brasiliensis roots.
abstract: Three types of roots (taproots, first order laterals and second order laterals) were functionally characterized on 7-month-old in vitro plantlets regenerated by somatic embryogenesis in Hevea brasiliensis. A histological analysis revealed different levels of differentiation depending on root diameter. A primary structure was found in first and second order lateral roots, while taproots displayed a secondary structure. The expression of 48 genes linked to some of the regulatory pathways acting in roots was compared in leaves, stems and the different types of roots by real-time RT-PCR. Thirteen genes were differentially expressed in the different organs studied in plants grown under control conditions. Nine additional other genes were differentially regulated between organs under water deficit conditions. In addition, 10 genes were significantly regulated in response to water deficit, including 8 regulated mainly in lateral roots types. Our results suggest that the regulation of gene expression in lateral roots is different than that in taproots, which have a main role in nutrient uptake and transport, respectively.
authors.affiliation: CIRAD, UMR DAP, F-34398 Montpellier, France.
authors.forename: Riza-Arief
authors.initials: RA
authors.lastname: Putranto
authors.forename: Christine
authors.initials: C
authors.lastname: Sanier
authors.forename: Julie
authors.initials: J
authors.lastname: Leclercq
authors.forename: Cuifang
authors.initials: C
authors.lastname: Duan
authors.forename: Maryannick
authors.initials: M
authors.lastname: Rio
authors.forename: Christophe
authors.initials: C
authors.lastname: Jourdan
authors.forename: Philippe
authors.initials: P
authors.lastname: Thaler
authors.forename: Xavier
authors.initials: X
authors.lastname: Sabau
authors.forename: Xavier
authors.initials: X
authors.lastname: Argout
authors.forename: Pascal
authors.initials: P
authors.lastname: Montoro
mesh_terms: D003681:Dehydration / Q000235:genetics*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D028482:Hevea / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D009928:Organ Specificity / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D018547:Plant Stems / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant