title: Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent.
abstract: Hemagglutinating activity has been associated to presence of lectin, carbohydrate-binding proteins. In this work Crataeva tapia bark lectin (CrataBL) was purified in milligram quantities (28 mg per g of bark) by ion exchange chromatography. The lectin was thermo-stable, ion-independent and N-terminal sequence analysis demonstrated similarity with miraculin and miraculin-like proteins (plant defensive proteins). Glycosylated nature of CrataBL was revealed using glycoprotein staining (periodic acid-Schiff's reagent), positive for polypeptides of apparent molecular masses 21 and 40 kDa on SDS-PAGE. Gel diffusion assay showed that glucose/mannose isolectins from Cratylia mollis recognized CrataBL glycan moiety. CrataBL hemagglutinating activity was inhibited by glycoproteins and CrataBL immobilized on cyanogen bromide-activated sepharose 4B (1 mL) bound 0.54 mg of glycoprotein (casein, fetuin and ovalbumin) per cycle. CrataBL was an insecticide agent against Nasutitermes corniger workers (termite that attack woods) with LC₅₀ of 0.475 mg mL⁻¹ for 6 days.
authors.affiliation: Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, Cidade Universitária, 50670-420 Recife, Pernambuco, Brazil.
authors.forename: Regina Maria Sousa de
authors.initials: RM
authors.lastname: Araújo
authors.forename: Rodrigo da Silva
authors.initials: Rda S
authors.lastname: Ferreira
authors.forename: Thiago Henrique
authors.initials: TH
authors.lastname: Napoleão
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Carneiro-da-Cunha
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maria Tereza Dos Santos
authors.initials: MT
authors.lastname: Correia
authors.forename: Maria Luiza Vilela
authors.initials: ML
authors.lastname: Oliva
authors.forename: Patrícia Maria Guedes
authors.initials: PM
authors.lastname: Paiva
mesh_terms: D000818:Animals; D031483:Capparaceae / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination; D007306:Insecticides / Q000737:chemistry* / Q000302:isolation & purification; D020049:Isoptera; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D007306:Insecticides; D010936:Plant Extracts; D037121:Plant Lectins