title: A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples.
abstract: A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.
authors.affiliation: Biofunctional Nanomaterials Department, CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Beloqui
authors.forename: Antonio
authors.initials: A
authors.lastname: Sanchez-Ruiz
authors.forename: Manuel
authors.initials: M
authors.lastname: Martin-Lomas
authors.forename: Niels-Christian
authors.initials: NC
authors.lastname: Reichardt
mesh_terms: D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009844:Oligosaccharides / Q000378:metabolism; D012463:Saliva / Q000201:enzymology; D012987:Soil; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009844:Oligosaccharides; D012987:Soil; D006026:Glycoside Hydrolases