title: Ultrasound-assisted lipase-catalyzed transesterification of soybean oil in organic solvent system.
abstract: This work reports the transesterification of soybean oil with ethanol using two commercial immobilized lipases under the influence of ultrasound irradiation. The experiments were performed in an ultrasonic water bath, following a sequence of experimental designs to assess the effects of temperature, enzyme and water concentrations, oil to ethanol molar ratio and output irradiation power on the reaction yield. Results show that ultrasound-assisted lipase-catalyzed transesterification of soybean oil with ethanol might be a potential alternative route to conventional alkali-catalyzed method, as high reaction yields (~90 wt.%) were obtained at mild irradiation power supply (~100 W), and temperature (60 °C) in a relatively short reaction time, 4h, using Lipozyme RM IM as catalyst. The repeated use of the catalyst under the optimum experimental condition resulted in a decay in both enzyme activity and product conversion after two cycles. The use of Novozym 435 led to lower conversions (about 57%) but the enzyme activity was stable after eight cycles of use, showing, however, a reduction in product conversion after the forth cycle.
authors.affiliation: Department of Food Engineering, URI - Campus de Erechim, Av Sete de Setembro, 1621 Erechim 99700-000, RS, Brazil.
authors.forename: Luciane
authors.initials: L
authors.lastname: Batistella
authors.forename: Lindomar A
authors.initials: LA
authors.lastname: Lerin
authors.forename: Patrícia
authors.initials: P
authors.lastname: Brugnerotto
authors.forename: Angélica J
authors.initials: AJ
authors.lastname: Danielli
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Trentin
authors.forename: Ariana
authors.initials: A
authors.lastname: Popiolski
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: J Vladimir
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D004789:Enzyme Activation / Q000528:radiation effects; D004800:Enzymes, Immobilized; D004951:Esterification / Q000528:radiation effects; D005656:Fungal Proteins; D019220:High-Energy Shock Waves; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000528:radiation effects*; D009930:Organic Chemicals / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D012997:Solvents / Q000737:chemistry / Q000528:radiation effects; D013010:Sonication / Q000379:methods*; D013024:Soybean Oil / Q000737:chemistry* / Q000528:radiation effects*; D013379:Substrate Specificity / Q000528:radiation effects
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D009930:Organic Chemicals; D012997:Solvents; D013024:Soybean Oil; C093667:Novozyme 435; D008049:Lipase