title: Concentrations of trace element in human dentin by sex and age.
abstract: Teeth are recently drawing attention for their potential as biological modeling investigation samples due to their ability to be collected and their slow substance metabolism. There is no active metabolism of elements after the completion of dentin. Dentin is surrounded by enamel and cementum, and is not affected by the oral environment. Therefore, the amount of trace elements in dentin may change with age, and this is considered to be a reliable biological load index. The objectives in this study are to demonstrate concentrations of elements in the dentin of healthy Japanese subjects by sex and age, and to reveal the relationship between element levels and age. 121 healthy teeth samples were extracted due to periodontal disease or orthodontic treatment. Each tooth was sliced from the crown to the root apex into 0.5-1mm thickness, then enamel, cementum, and the pulp were removed; the dentins were used as samples. The concentration of 10 trace elements (B, Mn, Co, Cu, Zn, Rb, Sr, Mo, Cd, and Pb) in the dentin was measured using inductively coupled plasma mass spectrometer (ICP-MS). The differences Co and Pb in the dentin between men and women were significant (p<0.01). Significant positive correlation was observed between B, Co, Cu, Zn, Sr and Pb concentrations in the dentin and age (p<0.001). The results of the present study suggest that human dentin is an appropriate substance for relativity with sex and age at further future research.
authors.affiliation: Division of Oral Surgery, Department of Oral and Maxillofacial Surgery, Iwate Medical University, Morioka, Japan. kumagaia@iwate-med.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Kumagai
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujita
authors.forename: Shigeatsu
authors.initials: S
authors.lastname: Endo
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Itai
mesh_terms: D000293:Adolescent; D000328:Adult; D000366:Age Determination by Teeth / Q000379:methods; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D044466:Asian People; D003804:Dentin / Q000737:chemistry*; D005260:Female; D005553:Forensic Dentistry; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D012737:Sex Factors; D014131:Trace Elements / Q000032:analysis*; D055815:Young Adult
chemical_list: D014131:Trace Elements