title: Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
abstract: We sought to assess the potential of human cord blood-derived mesenchymal stem cells (CB-MSCs) to derive insulin-producing, glucose-responsive cells. We show here that differentiation protocols based on stepwise culture conditions initially described for human embryonic stem cells (hESCs)  lead to differentiation of cord blood-derived precursors towards a pancreatic endocrine phenotype, as assessed by marker expression and in vitro glucose-regulated insulin secretion. Transplantation of these cells in immune-deficient animals shows human C-peptide production in response to a  glucose challenge. These data suggest that human cord blood may be a promising source for regenerative medicine approaches for the treatment of diabetes mellitus.
authors.affiliation: Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors.forename: Kamalaveni R
authors.initials: KR
authors.lastname: Prabakar
authors.forename: Juan
authors.initials: J
authors.lastname: Domínguez-Bendala
authors.forename: R Damaris
authors.initials: RD
authors.lastname: Molano
authors.forename: Antonello
authors.initials: A
authors.lastname: Pileggi
authors.forename: Susana
authors.initials: S
authors.lastname: Villate
authors.forename: Camillo
authors.initials: C
authors.lastname: Ricordi
authors.forename: Luca
authors.initials: L
authors.lastname: Inverardi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002096:C-Peptide / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D055098:Endocrine Cells / Q000166:cytology / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation; D050977:Maf Transcription Factors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D014157:Transcription Factors / Q000378:metabolism; D014183:Transplantation, Heterologous; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D015415:Biomarkers; D002096:C-Peptide; D007328:Insulin; D050977:Maf Transcription Factors; D014157:Transcription Factors; D005947:Glucose