title: Evaluation of a rapid culture-based screening test for detection of methicillin resistant Staphylococcus aureus.
abstract: The performance of a culture based assay, BacLite Rapid MRSA for the rapid detection (5 hours) of methicillin resistant Staphylococcus aureus (MRSA) from specimens (n = 377) obtained from nares, throat, wounds and perineum was investigated. Compared to culture based reference methods (chromogenic MRSA ID (bioMerieux)), selective enrichment broth, PBP2' latex agglutination (Oxoid) and VITEK 2 identification (bioMerieux), an overall sensitivity of 71% with a 82% specificity and a negative predictive value (NPV) of 95% was provided. The Baclite test is rapid and easy to use and has the advantage of a culture-based detection method for MRSA.
authors.affiliation: Institute of Laboratory Medicine and Microbiology, St.Joseph Hospital, Bremerhaven, Germany.
authors.forename: Boushra
authors.initials: B
authors.lastname: Fwity
authors.forename: Ralf
authors.initials: R
authors.lastname: Lobmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Ambrosch
mesh_terms: D002939:Ciprofloxacin / Q000494:pharmacology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D009666:Nose / Q000382:microbiology; D010502:Perineum / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D014456:Ulcer / Q000382:microbiology
chemical_list: D002939:Ciprofloxacin