title: NRP-1/CD304 expression in acute leukemia: a potential marker for minimal residual disease detection in precursor B-cell acute lymphoblastic leukemia.
abstract: Neuropilin-1 (NRP-1)/CD304 is a marker for plasmacytoid dendritic cells. We determined the distribution of NRP-1/CD304 expression on normal hematopoietic cells and in 167 acute leukemias by flow cytometry. NRP-1/CD304 surface expression was frequent in precursor B-cell acute lymphoblastic leukemia (36/51 [71%]) and uncommon in acute myeloid leukemia (22.9%). In acute myeloid leukemia, expression was noted in all (4/4) acute myeloid leukemias with the M4eo subtype and in 50% of specimens (6/12) with complex cytogenetics. On hematopoietic cells, NRP-1/CD304 was expressed on normal erythroid progenitors, plasma cells, and B-cell progenitors, as well as plasmacytoid dendritic cells. Expression was not consistently detected on other hematopoietic cell types. Owing to this distribution of expression, the detection of NRP-1/CD304 alone on a hematopoietic cell cannot be used to determine plasmacytoid dendritic cell differentiation. Finally, we show that NRP-1/CD304 is overexpressed in 30% of precursor B-cell acute lymphoblastic leukemia samples compared with normal B-cell progenitors, allowing for its potential use as a marker for the detection of minimal residual disease.
authors.affiliation: Department of Pathology, University Hospitals Case Medical Center, Cleveland, OH 44106, USA.
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Meyerson
authors.forename: Georgetta
authors.initials: G
authors.lastname: Blidaru
authors.forename: Alison
authors.initials: A
authors.lastname: Edinger
authors.forename: Ebenezer
authors.initials: E
authors.lastname: Osei
authors.forename: Karen
authors.initials: K
authors.lastname: Schweitzer
authors.forename: Pingfu
authors.initials: P
authors.lastname: Fu
authors.forename: Linda
authors.initials: L
authors.lastname: Ho
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D004900:Erythroblasts / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000378:metabolism; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000378:metabolism; D039942:Neuropilin-1 / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000378:metabolism; D054448:Precursor Cells, B-Lymphoid / Q000378:metabolism / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D039942:Neuropilin-1