title: Engineering Escherichia coli cells for cellobiose assimilation through a phosphorolytic mechanism.
abstract: We report here the first engineering effort for Escherichia coli biocatalysts to assimilate cellobiose through a phosphorolytic mechanism. Cytoplasmic expression of the Saccharophagus cellobiose phosphorylase was shown to enable E. coli to use cellobiose. Subsequent knockout and complementation studies provided solid evidence that the endogenous LacY was responsible for the transport of cellobiose.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
authors.forename: Ramanan
authors.initials: R
authors.lastname: Sekar
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Shin
authors.forename: Rachel
authors.initials: R
authors.lastname: Chen
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology / Q000235:genetics; D002475:Cellobiose / Q000378:metabolism*; D004738:Engineering; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D027981:Symporters; D002475:Cellobiose; D005964:Glucosyltransferases; C034714:cellobiose phosphorylase