title: Multiscale multimodal imaging with multiphoton microscopy and optical coherence tomography.
abstract: A multiscale multiphoton microscopy (MPM) and optical coherence tomography (OCT) system has been developed using a sub-10 fs Ti:sapphire laser. The system performs cross-sectional OCT imaging over millimeter field-of-view and en-face high-resolution MPM imaging with submicrometer resolution from the same sample location. With fish cornea, we have demonstrated cross-sectional imaging of cornea tissue layers using OCT, and the zoom-in imaging of cells and collagen fibers in each layer using MPM. The multiscale MPM/OCT system shows the potential of a rapid coarse scan to search for abnormal regions and the subsequent fine zoom-in imaging for diagnosis.
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC, Canada. tang@ece.ubc.ca
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Kenny K H
authors.initials: KK
authors.lastname: Chan
authors.forename: Tom
authors.initials: T
authors.lastname: Lai
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry; D003315:Cornea / Q000473:pathology* / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods*; D004867:Equipment Design; D005399:Fishes; D021621:Imaging, Three-Dimensional / Q000379:methods; D007834:Lasers; D044085:Microfluidics; D008853:Microscopy / Q000379:methods*; D008863:Microspheres; D055095:Optics and Photonics / Q000379:methods*; D012825:Silicon / Q000737:chemistry; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003094:Collagen; D012825:Silicon