title: Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters.
abstract: A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic bacterium, which stained Gram-positive but showed the typical cell wall structure of Gram-negative bacteria, was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. The strain, designated LIND7H(T), grew at 20-45 °C (optimum 35-40 °C) and at pH 5.0-8.5 (optimum pH 6.5-7.5). It did not require NaCl for growth, but was able to grow in the presence of up to 2 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain LIND7H(T) used cellobiose, glucose, lactose, mannose, maltose, peptone, rhamnose, raffinose, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were lactate, acetate, butyrate and isobutyrate. The predominant cellular fatty acids were anteiso-C(15 : 0), C(15 : 0), C(17 : 0) 2-OH and a summed feature consisting of C(18 : 2)ω6,9c and/or anteiso-C(18 : 0), and the major menaquinones were MK-9, MK-9(H(2)) and MK-10. The G+C content of the genomic DNA was 41.4 mol%. Although the closest phylogenetic relatives of strain LIND7H(T) were Parabacteroides merdae, Parabacteroides goldsteinii and Parabacteroides gordonii, analysis of the hsp60 gene sequence showed that strain LIND7H(T) was not a member of the genus Parabacteroides. On the basis of phylogenetic inference and phenotypic properties, strain LIND7H(T) ( = CCUG 60892(T) = DSM 23697(T) = JCM 16313(T)) is proposed as the type strain of a novel species in a new genus within the family Porphyromonadaceae, Macellibacteroides fermentans gen. nov., sp. nov.
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.|Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Linda
authors.initials: L
authors.lastname: Jabari
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Hana
authors.initials: H
authors.lastname: Gannoun
authors.affiliation: Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Abdeljabbar
authors.initials: A
authors.lastname: Hedi
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: CCUG, Culture Collection, Department of Clinical Bacteriology, University of Göteborg, 41346 Göteborg, Sweden.
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis Cedex, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Fauque
authors.affiliation: Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Aix-Marseille Universit� du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000003:Abattoirs; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014416:Tunisia; D024482:Vitamin K 2 / Q000032:analysis; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D062065:Wastewater