title: Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks.
abstract: Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-culture flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.
authors.forename: Dyana T
authors.initials: DT
authors.lastname: Saenz
authors.forename: Román
authors.initials: R
authors.lastname: Barraza
authors.forename: Nils
authors.initials: N
authors.lastname: Loewen
authors.forename: Wulin
authors.initials: W
authors.lastname: Teo
authors.forename: Eric M
authors.initials: EM
authors.lastname: Poeschla
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*