title: Regulation of universal stress protein genes by quorum sensing and RpoS in Burkholderia glumae.
abstract: Burkholderia glumae possesses a quorum-sensing (QS) system mediated by N-octanoyl-homoserine lactone (C(8)-HSL) and its cognate receptor TofR. TofR/C(8)-HSL regulates the expression of a transcriptional regulator, qsmR. We identified one of the universal stress proteins (Usps), Usp2, from a genome-wide analysis of QS-dependent proteomes of B. glumae. In the whole genome of B. glumae BGR1, 11 usp genes (usp1 to usp11) were identified. Among the stress conditions tested, usp1 and usp2 mutants died 1 h after heat shock stress, whereas the other usp mutants and the wild-type strain survived for more than 3 h at 45°C. The expressions of all usp genes were positively regulated by QS, directly by QsmR. In addition, the expressions of usp1 and usp2 were dependent on RpoS in the stationary phase, as confirmed by the direct binding of RpoS-RNA holoenzyme to the promoter regions of the usp1 and usp2 genes. The expression of usp1 was upregulated upon a temperature shift from 37°C to either 28°C or 45°C, whereas the expression of usp2 was independent of temperature stress. This indicates that the regulation of usp1 and usp2 expression is different from what is known about Escherichia coli. Compared to the diverse roles of Usps in E. coli, Usps in B. glumae are dedicated to heat shock stress.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hongsup
authors.initials: H
authors.lastname: Kim
authors.forename: Eunhye
authors.initials: E
authors.lastname: Goo
authors.forename: Yongsung
authors.initials: Y
authors.lastname: Kang
authors.forename: Jinwoo
authors.initials: J
authors.lastname: Kim
authors.forename: Ingyu
authors.initials: I
authors.lastname: Hwang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D019117:Burkholderia / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D004269:DNA, Bacterial / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D050296:Microbial Viability / Q000528:radiation effects; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D053038:Quorum Sensing; D012808:Sigma Factor / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria