title: Adsorption behavior of microbes on a QCM chip modified with an artificial siderophore-Fe3+ complex.
abstract: Three hydroxamate-type artificial siderophores with terminal NH(2) groups, tris[2-{3-(N-acyl-N-hydroxamino)propylamido}propyl]aminomethane (1-3, acyl-R group = Me, Et, and Ph, respectively), and their Fe(3+) complexes, 4-6, were prepared. The stability constant (log β) of 4 was estimated to be about 31 by its EDTA titration. The biological activities of 4-6 for Microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they permeated the cell membrane depending on their terminal bulky acyl-R groups. These artificial siderophore complexes, 4-6, were modified on Au electrode surfaces with the terminal NH(2) group (4-6/Au). The surface modification of 4-6 was confirmed by several electrochemical measurements. The quartz crystal microbalance (QCM) chips were also modified with 4-6. Microbe adsorption measurements using these modified QCM chips for M. flavescens, Pseudomonas putida, and Eschrichia coli were performed. The QCM chips have the ability to adsorb microbes selectively as a result of the differences in the interactions between the structures of Fe(3+)-artificial siderophore complexes and their receptors or binding proteins within the cell membrane.
authors.affiliation: Department of Material Science, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan. inomata.tomohiko@nitech.ac.jp
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Inomata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Eguchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Funahashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ozawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Masuda
mesh_terms: D000327:Adsorption; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011791:Quartz; D017262:Siderophores / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D017262:Siderophores; D011791:Quartz