title: The Srb1+1050T allele is associated with metabolic syndrome in children but not with cholesteryl ester plasma concentrations of high-density lipoprotein subclasses.
abstract: BACKGROUND
  Low cholesterol and phospholipid plasma levels of some high-density lipoprotein (HDL) subclasses have been described in children with metabolic syndrome. Scavenger receptor class B type I (SR-BI) has been proposed to be at the origin of such HDL alterations because of its key role on cholesteryl esters-HDL metabolism. However, the possible contribution of SR-BI has not been specifically explored in this kind of patients.
  
  
  METHODS
  Plasma lipid concentrations of HDL subclasses, i.e., triglycerides (TG), phosphatidylcholine (Ph), free cholesterol (FC), and total cholesterol (TC), were determined by enzymatic staining on polyacrylamide gradient gels (PAGE) in 39 pediatric patients with metabolic syndrome and 65 children as controls. Cholesteryl esters were estimated by the difference between TC and FC. Proteins of HDL subclasses were also stained for the assessment of the relative size distribution of HDL. For statistical analysis, the study population was grouped by Srb1 +1050C-->T polymorphism (rs5888) as carriers or noncarriers of the T allele, and data were corrected by metabolic syndrome status.
  
  
  RESULTS
  The Srb1 +1050T allele was associated with metabolic syndrome [odds ratio (OR)=2.18 (1.12-4.22), P=0.02]. Plasma TG corresponding to HDL3a, as well as the relative proportion of this HDL subclass, were slightly higher in carriers of the T allele as compared to CC homozygous subjects. Cholesteryl esters plasma concentrations of all HDL subclasses were comparable between T allele carriers and noncarriers after correction by metabolic syndrome status.
  
  
  CONCLUSIONS
  Srb1 +1050T was associated with metabolic syndrome, but T carrier subjects did not show important differences concerning HDL subclasses as compared to noncarriers.
authors.affiliation: Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
authors.forename: Minerva
authors.initials: M
authors.lastname: Juárez-Meavepeña
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Carreón-Torres
authors.forename: Cristhel
authors.initials: C
authors.lastname: López-Osorio
authors.forename: Cynthia
authors.initials: C
authors.lastname: García-Sánchez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gamboa
authors.forename: Margarita
authors.initials: M
authors.lastname: Torres-Tamayo
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Fragoso
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rodríguez-Pérez
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: Oscar
authors.initials: O
authors.lastname: Pérez-Méndez
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002788:Cholesterol Esters / Q000097:blood* / Q000145:classification; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000145:classification; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D009994:Osmolar Concentration; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D051122:Scavenger Receptors, Class B / Q000235:genetics*
chemical_list: D002788:Cholesterol Esters; D008075:Lipoproteins, HDL; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B