title: Strain construction and screening methods for a yeast histone H3/H4 mutant library.
abstract: A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Dai
authors.forename: Jef D
authors.initials: JD
authors.lastname: Boeke
mesh_terms: D015723:Gene Library; D017930:Genes, Reporter / Q000235:genetics; D005820:Genetic Testing / Q000379:methods*; D006657:Histones / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D006657:Histones; D029701:Saccharomyces cerevisiae Proteins