title: [In vitro and in vivo effects of ingavirin on the ultrastructure and infectivity of influenza virus].
abstract: The aim of this investigation was to study the effect of ingavirin on the structure and properties of influenza virions forming in its presence. The infectious activity of the virus and the morphology of the virions were analyzed by titration in cell culture and electron microscopy, respectively. The use of ingavirin was shown to reduce the proportion of morphologically intact virions and to increase that of filamentous and giant particles. No defects of surface glycoproteins were observed. The effect of the drug did not depend on the chosen model of virus replication and it was similarly shown in both cultured human cells and laboratory animals. In MDCK and A549 cells and in the mouse lungs, viral infectious activity was decreased by 1-2 orders of magnitude in relation to a model. The findings suggest that Ingavirin is able to impair the processes of viral morphogenesis, which in turn leads to a reduction in the infectivity of progeny virions.
authors.forename: V V
authors.initials: VV
authors.lastname: Zarubaev
authors.forename: S V
authors.initials: SV
authors.lastname: Beliaevskaia
authors.forename: A K
authors.initials: AK
authors.lastname: Sirotkin
authors.forename: P M
authors.initials: PM
authors.lastname: Anfimov
authors.forename: V E
authors.initials: VE
authors.lastname: Nebol'sin
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
authors.forename: D V
authors.initials: DV
authors.lastname: Reĭkhart
mesh_terms: D000577:Amides / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002208:Caproates; D002460:Cell Line; D003998:Dicarboxylic Acids / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology* / Q000627:therapeutic use; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007251:Influenza, Human / Q000188:drug therapy; D051379:Mice; D014771:Virion / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D014774:Virulence / Q000187:drug effects*
chemical_list: D000577:Amides; D000998:Antiviral Agents; D002208:Caproates; D003998:Dicarboxylic Acids; D007093:Imidazoles; C539419:pentanedioic acid imidazolyl ethanamide