title: Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii).
abstract: To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate γ-linolenic acid (18:3 n-6) to di-homo-γ-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)Δ6/C(20)Δ5 but not C(22) PUFA substrates.
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Seodun-Dong, Suwon, 441-707, Korea. sunheekim00@korea.kr
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: Yo Soon
authors.initials: YS
authors.lastname: Jang
authors.forename: So Yun
authors.initials: SY
authors.lastname: Kim
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Roh
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Kim
authors.forename: Kyeong-Ryeol
authors.initials: KR
authors.lastname: Lee
authors.forename: Jong-Sug
authors.initials: JS
authors.lastname: Park
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D021541:Sea Bream; D017422:Sequence Analysis, DNA; D017965:gamma-Linolenic Acid / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D017965:gamma-Linolenic Acid; D044943:Fatty Acid Desaturases