title: Simultaneous quantitative determination and validation of quercetin glycosides with peroxynitrite-scavenging effects from Saussurea grandifolia.
abstract: The leaves of Saussurea grandifolia (Compositae) are used as chwinamul, a well-known Korean mountainous vegetable, or to treat hepatitis and hematuria. Since the methanolic extract of S. grandifolia leaves exhibit a potent peroxynitrite-scavenging effect, phytochemical and high-performance liquid chromatographic (HPLC) analyses were employed to identify and simultaneously quantify the active components: chlorogenic acid and 3,5-di-O-caffeoylquinic acid (3,5-DQ) as caffeoylquinic acids, and quercetin, isoquercitrin (quercetin-3-glucoside), saxifragin (quercetin-5-glucoside), and rutin (quercetin-3-rutinoside). Validation of HPLC methods was performed to verify the linearity, LOD, LOQ, intra-day and inter-day variabilities, recovery, and repeatability to ensure that this method is selective, sensitive, precise, accurate and reproducible. In particular, leaves contained saxifragin with potent peroxynitrite-scavenging activity (IC(50), 0.67 μM) as 4.65 mg/g dry weight, which is equivalent to 33.74 mg/g extract.
authors.affiliation: Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju 220-702, Republic of Korea.
authors.forename: Agung
authors.initials: A
authors.lastname: Nugroho
authors.forename: Sang-Cheol
authors.initials: SC
authors.lastname: Lim
authors.forename: Chan Mi
authors.initials: CM
authors.lastname: Lee
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000592:standards*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D016166:Free Radical Scavengers / Q000032:analysis* / Q000737:chemistry; D006027:Glycosides / Q000032:analysis; D030421:Peroxynitrous Acid / Q000032:analysis* / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis*; D015203:Reproducibility of Results; D030441:Saussurea / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D006027:Glycosides; D010936:Plant Extracts; D030421:Peroxynitrous Acid; D011794:Quercetin