title: Cleavage of oligonucleotides containing a P3'→N5' phosphoramidate linkage mediated by single-stranded oligonucleotide templates.
abstract: Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3'→N5' phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kosuke Ramon
authors.initials: KR
authors.lastname: Ito
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kodama
authors.forename: Futaba
authors.initials: F
authors.lastname: Makimura
authors.forename: Noritsugu
authors.initials: N
authors.lastname: Hosoki
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Osaki
authors.forename: Ayako
authors.initials: A
authors.lastname: Orita
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imanishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
mesh_terms: D000577:Amides / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000528:radiation effects; D006868:Hydrolysis / Q000528:radiation effects; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010756:Phosphoric Acids / Q000737:chemistry* / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013698:Templates, Genetic; D013816:Thermodynamics; D014466:Ultraviolet Rays
chemical_list: D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; D012313:RNA; D004247:DNA; C011067:phosphoramidic acid