title: A comparative lipidomics platform for chemotaxonomic analysis of Mycobacterium tuberculosis.
abstract: The lipidic envelope of Mycobacterium tuberculosis promotes virulence in many ways, so we developed a lipidomics platform for a broad survey of cell walls. Here we report two new databases (MycoMass, MycoMap), 30 lipid fine maps, and mass spectrometry datasets that comprise a static lipidome. Further, by rapidly regenerating lipidomic datasets during biological processes, comparative lipidomics provides statistically valid, organism-wide comparisons that broadly assess lipid changes during infection or among clinical strains of mycobacteria. Using stringent data filters, we tracked more than 5,000 molecular features in parallel with few or no false-positive molecular discoveries. The low error rates allowed chemotaxonomic analyses of mycobacteria, which describe the extent of chemical change in each strain and identified particular strain-specific molecules for use as biomarkers.
authors.affiliation: Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Emilie
authors.initials: E
authors.lastname: Layre
authors.forename: Lindsay
authors.initials: L
authors.lastname: Sweet
authors.forename: Sunhee
authors.initials: S
authors.lastname: Hong
authors.forename: Cressida A
authors.initials: CA
authors.lastname: Madigan
authors.forename: Danielle
authors.initials: D
authors.lastname: Desjardins
authors.forename: David C
authors.initials: DC
authors.lastname: Young
authors.forename: Tan-Yun
authors.initials: TY
authors.lastname: Cheng
authors.forename: John W
authors.initials: JW
authors.lastname: Annand
authors.forename: Keunpyo
authors.initials: K
authors.lastname: Kim
authors.forename: Isdore C
authors.initials: IC
authors.lastname: Shamputa
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: McConnell
authors.forename: C Anthony
authors.initials: CA
authors.lastname: Debono
authors.forename: Samuel M
authors.initials: SM
authors.lastname: Behar
authors.forename: Adriaan J
authors.initials: AJ
authors.lastname: Minnaard
authors.forename: Megan
authors.initials: M
authors.lastname: Murray
authors.forename: Clifton E
authors.initials: CE
authors.lastname: Barry
authors.forename: Isamu
authors.initials: I
authors.lastname: Matsunaga
authors.forename: D Branch
authors.initials: DB
authors.lastname: Moody
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D016208:Databases, Factual; D008055:Lipids / Q000032:analysis*; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D015415:Biomarkers; D008055:Lipids