title: N-to-C sequential ligation using peptidyl N,N-bis(2-mercaptoethyl)amide building blocks.
abstract: A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin.
authors.affiliation: Structural Biology and Biochemistry Division, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
authors.forename: Renliang
authors.initials: R
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Hou
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Liu
mesh_terms: D000577:Amides / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D015394:Molecular Structure; D009584:Nitrogen / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D000577:Amides; D010455:Peptides; D025801:Ubiquitin; D002244:Carbon; D009584:Nitrogen