title: Lettuce necrotic yellows cytorhabdovirus protein localization and interaction map, and comparison with nucleorhabdoviruses.
abstract: Lettuce necrotic yellows virus (LNYV), Sonchus yellow net virus (SYNV) and Potato yellow dwarf virus (PYDV) are members of the family Rhabdoviridae that infect plants. LNYV is a cytorhabdovirus that replicates in the cytoplasm, while SYNV and PYDV are nucleorhabdoviruses that replicate in the nuclei of infected cells. LNYV and SYNV share a similar genome organization with a gene order of nucleoprotein (N), phosphoprotein (P), putative movement protein (Mv), matrix protein (M), glycoprotein (G) and polymerase (L). PYDV contains an additional predicted gene of unknown function located between N and P. In order to gain insight into the associations of viral structural and non-structural proteins and the mechanisms by which they may function, we constructed protein localization and interaction maps. Subcellular localization was determined by transiently expressing the viral proteins fused to green or red fluorescent protein in leaf epidermal cells of Nicotiana benthamiana. Protein interactions were tested in planta by using bimolecular fluorescence complementation. All three viruses showed Mv to be localized at the cell periphery and the G protein to be membrane associated. Comparing the interaction maps revealed that only the N-P and M-M interactions are common to all three viruses. Associations unique to only one virus include P-M for LNYV, G-Mv for SYNV and M-Mv, M-G and N-M for PYDV. The cognate N-P proteins of all three viruses interacted and exhibited characteristic changes in localization when co-expressed.
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Martin
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia Qld 4072, Australia.|Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Dietzgen
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Renyuan
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Goodin
mesh_terms: D002467:Cell Nucleus / Q000821:virology; D004721:Endoplasmic Reticulum / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D018613:Microscopy, Confocal; D010942:Plant Viruses / Q000235:genetics* / Q000502:physiology; D012209:Rhabdoviridae / Q000235:genetics* / Q000502:physiology; D014026:Nicotiana / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins