title: [Effects of miR-122 on expression of hepatitis B virus proteins].
abstract: To investigate the effect of miR-122 on the expression of hepatitis B virus (HBV) proteins.
  
  
  
  Anti-sense oligodeoxynucleotide (ASODN) of two different sequences against miR-122, anti-miR-122 and LNA-antimiR-122 (Locked nucleic acid), human miR -122 (hsa-miR-122), or the negative control anti-GFP were designed and synthesized, then transfected into HepG2.2.15 cells. After 24 h and 48 h, the levels of HBsAg and HBeAg in the supernatant were determined with a time-resolved immunofluorometric assay (TRFIA). HBV DNA in supernatant and miR-122 in cells were measured by quantitative real-time PCR.
  
  
  
  After 48 h expressions of miR-122 in the LNA-antimiR-122 and anti-miR-122 groups were significantly suppressed and lower than those in the negative control (P<0.001), while the level of miR-122 in the hsa-miR-122 group was higher than that in the negative control (P<0.001). The expression of HBeAg and HBsAg in hsa-miR-122 group was lower than that in the negative control (P<0.01) 24 h and 48 h after transfection. The expression of HBeAg and HBsAg in the anti-miR-122 group and LNA-antimiR-122 group was significantly lower than that in negative control (P>0.001). The levels of viral DNA at both time-points in the various test groups were not significantly different from those of negative control group (P>0.05).
  
  
  
  miR-122 may regulate HBV antigens and potentially affect the progress of pathogenesis, which might be the new targets for treatment of HBV infection.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zhong-rong
authors.initials: ZR
authors.lastname: Hu
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Chen
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Liu
authors.forename: Min-jun
authors.initials: MJ
authors.lastname: Hu
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Zhu
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; C531907:MIRN122 microRNA, human; D035683:MicroRNAs