title: [Detection of antibodies to Campylobacter jejuni in pediatrics patients with Gullain-Barré syndrome using different antigen preparations].
abstract: Diagnosis of previous C. jejuni infections in GBS patients are mostly based on serological findings. However, there are not consensus what kind of antigen should be used in the serological assays. In our study we used ELISA with four different antigen preparations for investigation of specific antibodies to C. jejuni in serum samples obtained from 6 children with GBS. In all patients the high level of IgA and IgG antibodies to LPS were diagnosed. The antibodies in particular classes of immunoglobulins to recombinant proteins (Mikrogen), termostabile antigen and whole-cell antigen (Virion/Serion) of C. jejuni were diagnosed only in some of the children with GBS. However, in comparison to the control groups, the ELISA with recombinant proteins was most specific. Moreover, in two of the patients a characteristic decline of the level of antibodies to recombinant proteins in sera obtained in acute and chronic phase of disease have been observed. The results of this study showed that serodiagnosis, specially based on recombinant antigens, may be a reliable marker of recent or previous infection caused by C. jejuni in patients with GBS.
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakładu Higieny w Warszawie.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rokosz
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rastawicki
authors.forename: Marek
authors.initials: M
authors.lastname: Jagielski
authors.forename: Zofia
authors.initials: Z
authors.lastname: Hetkowska-Abramczyk
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D002169:Campylobacter Infections / Q000276:immunology*; D016123:Campylobacter jejuni / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003509:Cyclohexanecarboxylic Acids / Q000097:blood; D020275:Guillain-Barre Syndrome / Q000150:complications* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D003509:Cyclohexanecarboxylic Acids; D007074:Immunoglobulin G; C045466:IG 10