title: Erythropoietin promotes oligodendrogenesis and myelin repair following lysolecithin-induced injury in spinal cord slice culture.
abstract: Here, we sought to delineate the effect of EPO on the remyelination processes using an in vitro model of demyelination. We report that lysolecithin-induced demyelination elevated EPO receptor (EpoR) expression in oligodendrocyte progenitor cells (OPCs), facilitating the beneficial effect of EPO on the formation of oligodendrocytes (oligodendrogenesis). In the absence of EPO, the resultant remyelination was insufficient, possibly due to a limiting number of oligodendrocytes rather than their progenitors, which proliferate in response to lysolecithin-induced injury. By EPO treatment, lysolecithin-induced proliferation of OPCs was accelerated and the number of myelinating oligodendrocytes and myelin recovery was increased. EPO also enhanced the differentiation of neural progenitor cells expressing EpoR at high level toward the oligodendrocyte-lineage cells through activation of cyclin E and Janus kinase 2 pathways. Induction of myelin-forming oligodendrocytes by high dose of EPO implies that EPO might be the key factor influencing the final differentiation of OPCs. Taken together, our data suggest that EPO treatment could be an effective way to enhance remyelination by promoting oligodendrogenesis in association with elevated EpoR expression in spinal cord slice culture after lysolecithin-induced demyelination.
authors.affiliation: Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, South Korea.
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Cho
authors.forename: Gunha
authors.initials: G
authors.lastname: Kim
authors.forename: Serah
authors.initials: S
authors.lastname: Park
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Sim
authors.forename: You Jin
authors.initials: YJ
authors.lastname: Won
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Hwang
authors.forename: Jong Yoon
authors.initials: JY
authors.lastname: Yoo
authors.forename: Hea Nam
authors.initials: HN
authors.lastname: Hong
mesh_terms: D000818:Animals; D003711:Demyelinating Diseases / Q000139:chemically induced; D004921:Erythropoietin / Q000494:pharmacology*; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D009186:Myelin Sheath / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects*; D009836:Oligodendroglia / Q000166:cytology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D017467:Receptors, Erythropoietin / Q000819:agonists / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology; D058630:Spinal Cord Regeneration / Q000187:drug effects*
chemical_list: D008244:Lysophosphatidylcholines; D017467:Receptors, Erythropoietin; D004921:Erythropoietin