title: In vitro anti-viral effect of β-santalol against influenza viral replication.
abstract: The anti-influenza A/HK (H3N2) virus activity of β-santalol was evaluated in MDCK cells and investigated the effect of β-santalol on synthesis of viral mRNAs. β-Santalol was investigated for its antiviral activity against influenza A/HK (H3N2) virus using a cytopathic effect (CPE) reduction method. β-Santalol exhibited anti-influenza A/HK (H3N2) virus activity of 86% with no cytotoxicity at the concentration of 100 μg/ml reducing the formation of a visible CPE. Oseltamivir also showed moderate antiviral activity of about 83% against influenza A/HK (H3N2) virus at the concentration of 100 μg/ml. Furthermore, the mechanism of anti-influenza virus action in the inhibition of viral mRNA synthesis was analyzed by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), and the data indicated an inhibitory effect in late viral RNA synthesis compared with oseltamivir in the presence of 100 μg/ml of β-santalol. β-Santalol should be further studied for therapeutic and prophylactic potential especially for influenza epidemics and pandemics.
authors.affiliation: Proteomics and Molecular Cell Physiology Lab, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, TN, India. curlyproteomics@gmail.com
authors.forename: Manickam
authors.initials: M
authors.lastname: Paulpandi
authors.forename: Soundarapandian
authors.initials: S
authors.lastname: Kannan
authors.forename: Ramar
authors.initials: R
authors.lastname: Thangam
authors.forename: Krishnasamy
authors.initials: K
authors.lastname: Kaveri
authors.forename: Palani
authors.initials: P
authors.lastname: Gunasekaran
authors.forename: Chandrababu
authors.initials: C
authors.lastname: Rejeeth
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004285:Dogs; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D008826:Microbial Sensitivity Tests; D053139:Oseltamivir / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D000081223:Polycyclic Sesquiterpenes; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032123:Santalum / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010938:Plant Oils; D000081223:Polycyclic Sesquiterpenes; D012333:RNA, Messenger; D012367:RNA, Viral; D012717:Sesquiterpenes; C043206:santalol; D053139:Oseltamivir; C076763:sandalwood oil