title: Proteome profile and biological activity of caprine, bovine and human milk fat globules.
abstract: Upon combining bidimensional electrophoresis with monodimensional separation, a more comprehensive analysis of the milk fat globule membrane has been obtained. The proteomic profile of caprine milk fat globules revealed the presence of butyrophilin, lactadherin and perilipin as the major proteins, they were also associated to bovine and human milk fat globule membranes. Xanthine dehydrogenase/oxidase has been detected only in monodimensional gels. Biological activity of milk fat globules has been evaluated in Caco2-cells, as a representative model of the intestinal barrier. The increase of cell viability was indicative of a potential nutraceutical role for the whole milk fat globule, suggesting a possible employment in milk formula preparation.
authors.affiliation: Department of Environmental and Life Sciences, University of Piemonte Orientale, Viale T. Michel, 11, 15121 Alessandria, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Spertino
authors.forename: Valentina
authors.initials: V
authors.lastname: Cipriani
authors.forename: Chiara
authors.initials: C
authors.lastname: De Angelis
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: Francesco
authors.initials: F
authors.lastname: Marsano
authors.forename: Maria
authors.initials: M
authors.lastname: Cavaletto
mesh_terms: D000818:Animals; D000071797:Butyrophilins; D018938:Caco-2 Cells; D002417:Cattle; D002470:Cell Survival; D019587:Dietary Supplements; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006041:Goats; D006801:Humans; D066292:Lipid Droplets; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D008895:Milk, Human / Q000737:chemistry*; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D014968:Xanthine Dehydrogenase / Q000032:analysis; D014969:Xanthine Oxidase / Q000032:analysis
chemical_list: D000071797:Butyrophilins; D006017:Glycolipids; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; D020543:Proteome; C110367:milk fat globule; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase