title: Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes.
abstract: Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Hurlingham, Buenos Aires, Argentina.
authors.forename: Ariel A
authors.initials: AA
authors.lastname: Bazzini
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Manacorda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tohge
authors.forename: Gabriela
authors.initials: G
authors.lastname: Conti
authors.forename: Maria C
authors.initials: MC
authors.lastname: Rodriguez
authors.forename: Adriano
authors.initials: A
authors.lastname: Nunes-Nesi
authors.forename: Sofía
authors.initials: S
authors.lastname: Villanueva
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Fernando
authors.initials: F
authors.lastname: Carrari
authors.forename: Sebastian
authors.initials: S
authors.lastname: Asurmendi
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D018515:Plant Leaves / Q000382:microbiology; D015398:Signal Transduction / Q000235:genetics; D018709:Statistics, Nonparametric; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000502:physiology*
chemical_list: D000596:Amino Acids; D035683:MicroRNAs