title: Elevated cross-talk between subchondral bone and cartilage in osteoarthritic joints.
abstract: Osteoarthritis (OA) is a degenerative joint disease and one of the leading causes of disability in the United States and across the world. As a disease of the whole joint, OA exhibits a complicated etiology with risk factors including, but not limited to, ageing, altered joint loading, and injury. Subchondral bone is hypothesized to be involved in OA development. However, direct evidence supporting this is lacking. We previously detected measurable transport of solute across the mineralized calcified cartilage in normal joints, suggesting a potential cross-talk between subchondral bone and cartilage. Whether this cross-talk exists in OA has not been established yet. Using two models that induced OA by either ageing or surgery (destabilization of medial meniscus, DMM), we tested the hypothesis that increased cross-talk occurs in OA. We quantified the diffusivity of sodium fluorescein (mol. wt. 376Da), a marker of small-sized signaling molecules, within calcified joint matrix using our newly developed fluorescence loss induced by photobleaching (FLIP) method. Tracer diffusivity was found to be 0.30±0.17 and 0.33±0.20μm(2)/s within the calcified cartilage and 0.12±0.04 and 0.07±0.03μm(2)/s across the osteochondral interface in the aged (20-24-month-old, n=4) and DMM OA joints (5-month-old, n=5), respectively, which were comparable to the control values for the contralateral non-operated joints in the DMM mice (0.48±0.13 and 0.12±0.06μm(2)/s). Although we did not detect significant changes in tissue matrix permeability in OA joints, we found i) an increased number of vessels invading the calcified cartilage (and sometimes approaching the tidemark) in the aged (+100%) and DMM (+50%) joints relative to the normal age controls; and ii) a 60% thinning of the subchondral bone and calcified cartilage layers in the aged joints (with no significant changes detected in the DMM joints). These results suggested that the capacity for cross-talk between subchondral bone and articular cartilage could be elevated in OA. Further studies are needed to identify the direction of the cross-talk, the signaling molecules involved, and to test whether subchondral bone initiates OA development and could serve as a pharmaceutical target for OA treatment. This article is part of a Special Issue entitled "Osteoarthritis".
authors.affiliation: Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, PR China. panj@cqu.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Zhou
authors.forename: Thomas
authors.initials: T
authors.lastname: Scherr
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Yang
authors.forename: Christopher
authors.initials: C
authors.lastname: Price
authors.forename: Liyun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000473:pathology*; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D004838:Epiphyses / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D005269:Femur / Q000098:blood supply / Q000473:pathology; D019793:Fluorescein / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007596:Joints / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010003:Osteoarthritis / Q000473:pathology*; D010539:Permeability; D038761:Photobleaching; D015203:Reproducibility of Results; D013194:Staining and Labeling
chemical_list: D019793:Fluorescein