title: [Diagnostic use of D2-40 and annexin-1 in ependymal tumors].
abstract: OBJECTIVE
  To investigate the diagnostic significance of D2-40 and annexin-1 in the ependymal tumors.
  
  
  METHODS
  To analyses the expression of D2-40, annexin-1, EMA and Ki-67 by immunohistochemistry in 52 cases of ependymal tumors (48 cases of ependymomas, 4 cases of choroid plexus papilloma) from Xuanwu Hospital from 2005 to 2009. Ten cases of corresponding normal brain tissue were also obtained as control.
  
  
  RESULTS
  Thirty-two of forty-eight (66.7%) cases of ependymomas were positive for D2-40. "Dot-like" and "ring-like" structures were commonly observed in ependymomas (55.3%, 21 of 38 cases) and anaplastic ependymomas (5 of 6 cases) with D2-40 staining. There was no difference in the expression between D2-40 and Ki-67 (r(s) = -0.013, P = 0.931). For annexin-1, 87.5% (42 of 48 cases) of the ependymomas were positive. The specific "granular structures" and cilium were observed in ependymomas (1 of 4 cases of myxopapillary ependymomas and 11 of 38 cases of ependymomas respectively) for annexin-1. The difference in expression between annexin-1 and Ki-67 was statistically significant (r(s) = -0.405, P = 0.005). D2-40 in combination of EMA and annexin-1 increased the positive rate to 100% in ependymomas. Choroid plexus papillomas were all positive for D2-40 and annexin-1. The control tissue was negative for D2-40 but positive for annexin-1 in the capillaries.
  
  
  CONCLUSIONS
  The specific structures are valuable in diagnosing of ependymal-genetic tumors, and are highlighted by D2-40 and annexin-1. D2-40 in combination of EMA and annexin-1 is a useful diagnostic marker for ependymal tumors.
authors.affiliation: Department of Neuropathology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Yue-feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Yue-shan
authors.initials: YS
authors.lastname: Piao
authors.forename: De-hong
authors.initials: DH
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D017305:Annexin A1 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004806:Ependymoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D018396:Mucin-1 / Q000378:metabolism; D020288:Papilloma, Choroid Plexus / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D055815:Young Adult; D000098853:Podoplanin
chemical_list: D017305:Annexin A1; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D018396:Mucin-1; C117468:PDPN protein, human; D000098853:Podoplanin