title: Gene expression profiling of blood in brain arteriovenous malformation patients.
abstract: Brain arteriovenous malformations (BAVMs) are an important cause of intracranial hemorrhage (ICH) in young adults. Gene expression profiling of blood has led to the identification of stroke biomarkers, and may help identify BAVM biomarkers and illuminate BAVM pathogenesis. It is unknown whether blood gene expression profiles differ between 1) BAVM patients and healthy controls, or 2) unruptured and ruptured BAVM patients at presentation. We characterized blood transcriptional profiles in 60 subjects (20 unruptured BAVM, 20 ruptured BAVM, and 20 healthy controls) using Affymetrix whole genome expression arrays. Expression differences between groups were tested by ANOVA, adjusting for potential confounders. Genes with absolute fold change ≥ 1.2 (false discovery rate corrected p ≤ 0.1) were selected as differentially expressed and evaluated for over-representation in KEGG biological pathways (p ≤ 0.05). Twenty-nine genes were differentially expressed between unruptured BAVM patients and controls, including 13 which may be predictive of BAVM. Patients with ruptured BAVM compared to unruptured BAVM differed in expression of 1490 genes, with over-representation of genes in 8 pathways including MAPK, VEGF, Wnt signaling and several inflammatory pathways. These results suggest clues to the pathogenesis of BAVM and/or BAVM rupture and point to potential biomarkers or new treatment targets.
authors.affiliation: Center for Cerebrovascular Research, Department of Anesthesia and Perioperative Care, University of California, San Francisco, San Francisco, CA.
authors.forename: Shantel M
authors.initials: SM
authors.lastname: Weinsheimer
authors.forename: Huichun
authors.initials: H
authors.lastname: Xu
authors.forename: Achal S
authors.initials: AS
authors.lastname: Achrol
authors.forename: Boryana
authors.initials: B
authors.lastname: Stamova
authors.forename: Charles E
authors.initials: CE
authors.lastname: McCulloch
authors.forename: Ludmila
authors.initials: L
authors.lastname: Pawlikowska
authors.forename: Yingfang
authors.initials: Y
authors.lastname: Tian
authors.forename: Nerissa U
authors.initials: NU
authors.lastname: Ko
authors.forename: Michael T
authors.initials: MT
authors.lastname: Lawton
authors.forename: Gary K
authors.initials: GK
authors.lastname: Steinberg
authors.forename: Steven D
authors.initials: SD
authors.lastname: Chang
authors.forename: Glen
authors.initials: G
authors.lastname: Jickling
authors.forename: Bradley P
authors.initials: BP
authors.lastname: Ander
authors.forename: Helen
authors.initials: H
authors.lastname: Kim
authors.forename: Frank R
authors.initials: FR
authors.lastname: Sharp
authors.forename: William L
authors.initials: WL
authors.lastname: Young