title: Magnesium and strontium doped octacalcium phosphate thin films by matrix assisted pulsed laser evaporation.
abstract: Octacalcium phosphate (OCP) is a promising alternative to hydroxyapatite as biomaterial for hard tissue repair. In this study we successfully applied Matrix Assisted Pulsed Laser Evaporation (MAPLE) to deposit Mg and Sr doped OCP (MgOCP and SrOCP), as well as OCP, thin films on titanium substrates. OCP, Mg-substituted and Sr-substituted OCP were synthesized in aqueous medium, then were suspended in deionised water, frozen at liquid nitrogen temperature and used as targets for MAPLE experiments. The depositions were carried out using a KrF* excimer laser source (λ=248 nm, τ(FWHM)=25 ns) in mild conditions of temperature and pressure. The results of X-ray diffraction, infrared spectroscopy, scanning electron microscopy and energy dispersive spectroscopy investigations revealed that the OCP thin films are deposited in the form of cauliflower-like aggregates and droplets, as well as crystal fragments, with a homogeneous distribution of magnesium and strontium on the surface of the coatings. Human osteoblast-like MG-63 cells were cultured on the different biomaterials up to 14days. MgOCP and SrOCP coatings promote osteoblast proliferation and differentiation with respect to OCP. Under these experimental conditions, the production of procollagen-type I, transforming growth factor-β1, alkaline phosphatase and osteocalcin indicated that the level of differentiation of the cells grown on the different coatings increased in the order OCP<MgOCP<SrOCP.
authors.affiliation: Department of Chemistry G. Ciamician, University of Bologna, 40126 Bologna, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Boanini
authors.forename: Paola
authors.initials: P
authors.lastname: Torricelli
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Felix
authors.initials: F
authors.lastname: Sima
authors.forename: Natalia
authors.initials: N
authors.lastname: Serban
authors.forename: Ion N
authors.initials: IN
authors.lastname: Mihailescu
authors.forename: Adriana
authors.initials: A
authors.lastname: Bigi
mesh_terms: D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007834:Lasers; D008274:Magnesium / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000648:ultrastructure; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D010590:Phalloidine / Q000378:metabolism; D033362:Powder Diffraction; D014176:Protein Biosynthesis; D013324:Strontium / Q000737:chemistry*; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; C022045:octacalcium phosphate; D010590:Phalloidine; D008274:Magnesium; D013324:Strontium