title: Overexpression of peptidyl-prolyl isomerase Pin1 attenuates hepatocytes apoptosis and secondary necrosis following carbon tetrachloride-induced acute liver injury in mice.
abstract: Pin1, a member of the parvulin family of PPIase enzymes, plays a crucial role in the post phosphorylation regulation that governs important roles in the cell signaling mechanism and regulates a variety of cellular events. In this study, we investigated the role of Pin1 in carbon tetrachloride (CCl(4))-induced apoptosis and necrosis of hepatocytes during acute liver injury of mice. An in vivo study was done with the overexpression of Pin1 in the mouse liver; using Pin1-adenoviruse (ad-Pin1) followed by CCl(4) injection to induce acute liver injury. Pin1 overexpression in the liver of the experimental mice attenuated acute liver injury induced by CCl(4) . Serum aminotransferases and the number of apoptotic cells were decreased compared to those of control virus injected mice. In addition, Pin1 overexpression increased NF-kB activity, as evidenced by increased DNA binding. In conclusion, Pin1 reduces acute liver injury of mice due to CCl(4) by modulating apoptotic signals and by increasing NF-kB activity.
authors.affiliation: Laboratory of Liver Regeneration, Research Institute of Clinical Medicine, Chonbuk National University Medical School, Duckjin-gu, Jeonju, Korea.
authors.forename: Prabodh
authors.initials: P
authors.lastname: Risal
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Park
authors.forename: Baik Hwan
authors.initials: BH
authors.lastname: Cho
authors.forename: Jae-Chun
authors.initials: JC
authors.lastname: Kim
authors.forename: Yeon Jun
authors.initials: YJ
authors.lastname: Jeong
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002251:Carbon Tetrachloride; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D009336:Necrosis; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism*; D000637:Transaminases / Q000097:blood; D015854:Up-Regulation / Q000235:genetics
chemical_list: D016328:NF-kappa B; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D002251:Carbon Tetrachloride; D000637:Transaminases; D019696:Peptidylprolyl Isomerase; C000606850:Pin1 protein, mouse