title: [Facial paralysis induced by herpes simplex virus type 1 and the study of facial neurons apoptosis in mice].
abstract: OBJECTIVE
  To study the apoptosis of facial motor neurons and the expression of apoptosis-related genes, Bcl-2 and Bax, in the animal model of viral facial paralysis.
  
  
  METHODS
  Total of 84 Balb/c mice were divided into viral inoculation group and nerve transaction group. The animals were executed 1, 3, 7, 10, 15, 20 and 30 days after being operated respectively. The histopathological features of facial neurons in brain stem were observed by HE and Nissl stain. The changes of facial neuronal apoptosis were observed by TUNEL. The changes of expression of Bcl-2 and Bax genes in facial neurons were observed by immunohistochemistry staining.
  
  
  RESULTS
  After nerve transection, increased apoptotic cells were found in homolateral facial motor nucleus and the peak appeared at 10 and 15 days. The level of Bcl-2 expression in neurons declined while the expression of Bax increased gradually. Correspondingly, the ratio of Bcl-2/Bax declined. In the viral inoculation group, no visible change of apoptosis and Bax expression, but the level of Bcl-2 and the ratio of Bcl-2/Bax increased gradually.
  
  
  CONCLUSIONS
  Comparing to axotomy, facial motor nucleus in HSV-1 infective animal model are free of apoptosis. Both the mild form of lesion and the ability to block apoptosis of HSV-1 are likely to be involved into the phenomenon. Bcl-2 and Bax might interfere with the apoptotic response.
authors.affiliation: Department of Otorhinolaryngology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Shen
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Feng
authors.forename: Yang
authors.initials: Y
authors.lastname: Zha
authors.forename: Yan-dong
authors.initials: YD
authors.lastname: Zhang
authors.forename: Pei-mao
authors.initials: PM
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D005158:Facial Paralysis / Q000473:pathology / Q000821:virology*; D005260:Female; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse