title: Gene expression profile of estrogen receptor alpha and beta in the ovaries of Zi geese (Anser cygnoides).
abstract: The profile of ERalpha and ERbeta gene expression in the ovaries of Zi geese at 1 day and 1,2, 3, 4, 5 and 8 months of age (n=8, respectively) was examined by quantitative real-time PCR (qRT-PCR). The results showed that the expression of ERalpha and ERbeta mRNA was greater at 1 to 5 and 8 months compared with that observed at 1 day. In particular, the level of expression of ERalpha and ERbeta at 8 months was greater, 2.47 +/- 0.23 fold and 29.07 +/- 1.25 fold, respectively, compared with that at 1 day (P<0.05). The expression of ERalpha mRNA was not significantly different at 1, 2, 3 and 4 months (P>0.05). The level of expression of ERalpha mRNA at 5 months was 1.86 +/- 0.17 fold higher than at 1 day (P<0.05). The level of expression of ERbeta mRNA at 2, 3, 4, 5 and 8 months (1.96 +/- 0.13, 2.58 +/- 0.08, 2.08 +/- 0.05, 3.25 +/- 0.11 and 29.07 +/- 1.25 fold, respectively, P<0.05) was significantly higher than at 1 day. In summary, the expression of ERalpha and ERbeta mRNA in the ovaries of geese was increased between newborn and the laying stage. These results suggest that ERalpha and ERbeta mediate the process of ovarian development and egg laying in geese. In addition, ERbeta may play a more important role in regulating the response of the ovary to estrogen during the developmental and egg-laying stages.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Kang
authors.forename: Dong Mei
authors.initials: DM
authors.lastname: Jiang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Rui Jin
authors.initials: RJ
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Zhen
authors.forename: Huan Min
authors.initials: HM
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D047628:Estrogen Receptor alpha / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D005777:Geese / Q000235:genetics*; D020869:Gene Expression Profiling; D010053:Ovary / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta