title: Evaluation of genetic diversity in Piper spp  using RAPD and SRAP markers.
abstract: Random amplified polymorphic DNA (RAPD) and  sequence-related amplified polymorphism (SRAP) analysis were applied to 74  individual plants of Piper spp in Hainan Island. The results showed that  the SRAP technique may be more informative and more efficient and effective for  studying genetic diversity of Piper spp than the RAPD technique. The  overall level of genetic diversity among Piper spp in Hainan was  relatively high, with the mean Shannon diversity index being 0.2822 and 0.2909,  and the mean Nei's genetic diversity being 0.1880 and 0.1947, calculated with  RAPD and SRAP data, respectively. The ranges of the genetic similarity  coefficient were 0.486-0.991 and 0.520-1.000 for 74 individual plants of Piper spp (the mean genetic distance was 0.505 and 0.480) and the within-species  genetic distance ranged from 0.063 to 0.291 and from 0.096 to 0.234, estimated  with RAPD and SRAP data, respectively. These genetic indices indicated that  these species are closely related genetically. The dendrogram generated with  the RAPD markers was topologically different from the dendrogram based on SRAP  markers, but the SRAP technique clearly distinguished all Piper spp from  each other. Evaluation of genetic variation levels of six populations showed  that the effective number of alleles, Nei's gene diversity and the Shannon  information index within Jianfengling and Diaoluoshan populations are higher  than those elsewhere; consequently conservation of wild resources of Piper in  these two regions should have priority.
authors.affiliation: Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, Hainan University, Ministry of Education, Haikou, Hainan Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: J-P
authors.initials: JP
authors.lastname: Liu
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D001947:Breeding; D002681:China; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D010802:Phylogeny; D031701:Piper / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers