title: Organ-specific cellular requirements for in vivo dendritic cell generation.
abstract: Bone marrow-derived dendritic cell (DC) precursors seed peripheral organs, where they encounter diverse cellular environments during their final differentiation into DCs. Flt3 ligand (Flt3-L) is critical for instructing DC generation throughout different organs. However, it remains unknown which cells produce Flt3-L and, importantly, which cellular source drives DC development in such a variety of organs. Using a novel BAC transgenic Flt3-L reporter mouse strain coexpressing enhanced GFP and luciferase, we show ubiquitous Flt3-L expression in organs and cell types. These results were further confirmed at the protein level. Although Flt3-L was produced by immune and nonimmune cells, the source required for development of the DC compartment clearly differed among organs. In lymphoid organs such as the spleen and bone marrow, Flt3-L production by hemopoietic cells was critical for generation of normal DC numbers. This was unexpected for the spleen because both immune and nonimmune cells equally contributed to the Flt3-L content in that organ. Thus, localized production rather than the total tissue content of Flt3-L in spleen dictated normal splenic DC development. No differences were observed in the number of DC precursors, suggesting that the immune source of Flt3-L promoted pre-cDC differentiation in spleen. In contrast, DC generation in the lung, kidney, and pancreas was mostly driven by nonhematopoietic cells producing Flt3-L, with little contribution by immune cells. These findings demonstrate a high degree of flexibility in Flt3-L-dependent DC generation to adapt this process to organ-specific cellular environments encountered by DC precursors during their final differentiation.
authors.affiliation: Division of Molecular Immunology, German Cancer Research Center, Heidelberg 60120, Germany.
authors.forename: Tewfik
authors.initials: T
authors.lastname: Miloud
authors.forename: Nathalie
authors.initials: N
authors.lastname: Fiegler
authors.forename: Janine
authors.initials: J
authors.lastname: Suffner
authors.forename: Günter J
authors.initials: GJ
authors.lastname: Hämmerling
authors.forename: Natalio
authors.initials: N
authors.lastname: Garbi
mesh_terms: D000818:Animals; D001853:Bone Marrow; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology*; D006412:Hematopoietic Stem Cells; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity
chemical_list: D008565:Membrane Proteins; C084881:flt3 ligand protein