title: Effects of 5-fluorouracil and gemcitabine on a breast cancer cell line (MCF-7) via the JAK/STAT pathway.
abstract: Aberrant activation of the JAK/STAT pathway may predispose to malignancy as a consequence of the deregulation of cell proliferation, differentiation or apoptosis such as in cancer of the blood, head and neck, and breast. In our study we aimed to investigate the effects of 5-fluorouracil (5-FU) and gemcitabine on a breast cancer cell line (MCF-7 cells) via the JAK/STAT pathway. Distribution of JAK1, JAK2, JAK3 and STAT2, STAT3, STAT4, STAT5 were evaluated on MCF-7 cells following gemcitabine and 5-FU treatment and in the absence of drug treatment by an indirect immunohistochemical method. It was observed that JAK1, JAK3, STAT5 and particularly STAT2 activation were more effective than the other JAK/STATs in breast cancer progression. Following treatment with 5-FU, JAK1 and STAT5 immunoreactivities were decreased in MCF-7 cells in comparison with both gemcitabine-treated and non-treated groups. These results suggest that the JAK/STAT pathway plays an important role in breast cancer pathogenesis and may be more affected after 5-FU treatment rather than gemcitabine. Drugs which block STAT5 may provide a novel therapeutic approach for the treatment of breast cancer.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Dekanlik Binasi, Uncubozkoy, Manisa, Turkey. drelginturkoz@gmail.com
authors.forename: Elgin Turkoz
authors.initials: ET
authors.lastname: Uluer
authors.forename: Isil
authors.initials: I
authors.lastname: Aydemir
authors.forename: Sevinc
authors.initials: S
authors.lastname: Inan
authors.forename: Kemal
authors.initials: K
authors.lastname: Ozbilgin
authors.forename: Hafize Seda
authors.initials: HS
authors.lastname: Vatansever
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D053612:Janus Kinases / Q000378:metabolism*; D050791:STAT Transcription Factors / Q000378:metabolism*; D015398:Signal Transduction; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D050791:STAT Transcription Factors; D003841:Deoxycytidine; D053612:Janus Kinases; D005472:Fluorouracil; D000093542:Gemcitabine