title: Identification and expression analysis of two splice variants of the 14-3-3 epsilon from Litopenaeus Vannamei during WSSV infections.
abstract: The 14-3-3 epsilon (14-3-3ε) is a member of the 14-3-3-protein family claimed to play important roles in many biological processes. In this study, two alternative 14-3-3 epsilon mRNAs, designated as 14-3-3EL and 14-3-3ES were identified from the shrimp L. vannamei. The 14-3-3EL isoform contains an insertion of 48 nucleotides by intron retention in the pre-mRNA of 14-3-3ε. While the 14-3-3ES occurred after being fully spliced. Using the yeast two hybrid method, the pattern of dimer formation by the two alternative 14-3-3ε isoforms revealed that the shrimp 14-3-3ε formed both homodimers and heterodimers. Both 14-3-3ε transcript variants were constitutively expressed in all shrimp tissues tested but the level of the 14-3-3ES isoform was always lower. However, after white spot syndrome virus (WSSV) infection, the expression level of the two transcript variants changed. At 48 h after infection, expression of 14-3-3EL mRNA increased significantly in the gill and muscle tissue whereas the expression 14-3-3ES increased only in muscle. It was of interest that in the lymphoid organ, there was a significant down-expression of both transcript variants. From these results we suggest that 14-3-3EL and 14-3-3ES might be related to different cellular processes that are modulated during virus infection.
authors.affiliation: Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand. wwanna_1@hotmail.com
authors.forename: Warapond
authors.initials: W
authors.lastname: Wanna
authors.forename: Jaturon
authors.initials: J
authors.lastname: Thipwong
authors.forename: Wiparat
authors.initials: W
authors.lastname: Mahakaew
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D033561:Penaeidae / Q000235:genetics* / Q000821:virology*; D055503:Protein Multimerization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014777:Virus Diseases / Q000235:genetics*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D048948:14-3-3 Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger