title: Development and characterization of 32 microsatellite loci in the giant grouper  Epinephelus lanceolatus (Serranidae).
abstract: An economically important marine fish species, the  giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in  Taiwan and costal areas of China. We isolated and characterized 32 polymorphic  microsatellite loci from a CA-enriched genomic library of giant grouper. The  number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed  and expected heterozygosities per locus varied from 0.387 to 1.000 and from  0.377 to 0.843, respectively. Six loci significantly deviated from  Hardy-Weinberg equilibrium. After sequential Bonferroni's correction, only two  loci showed deviation from Hardy-Weinberg equilibrium, and no linkage  disequilibrium was found between any pair of loci. These microsatellites can be  useful tools for the study of population genetics in the giant grouper.
authors.affiliation: State Key Laboratory of Biocontrol, Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: X C
authors.initials: XC
authors.lastname: Liu
authors.forename: H R
authors.initials: HR
authors.lastname: Lin
authors.forename: Z N
authors.initials: ZN
authors.lastname: Meng
mesh_terms: D000483:Alleles; D000818:Animals; D001492:Bass / Q000235:genetics*; D056426:Genetic Loci; D005828:Genetics, Population; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats