title: Metabolite profiles from dried blood spots for metabonomic studies using UPLC combined with orthogonal acceleration ToF-MS: effects of different papers and sample storage stability.
abstract: BACKGROUND
  Investigations have been undertaken of the effects of storage on the utility of using dried blood spots, collected and stored on a paper substrate, combined with reversed-phase ultra-performance LC (UPLC), electrospray ionization orthogonal acceleration ToF-MS (UPLC-oaToF-MS), as a method for performing global metabolic profiling studies. In addition, a range of products used to collect these blood spots have also been studied for effects on the UPLC-MS background.
  
  
  RESULTS
  These preliminary data suggest that sample stability in this application is limited unless the cards are stored at, at least, -20°C and preferably at -80°C. In addition, the use of untreated cards is recommended to minimize incurred background interferences.
  
  
  CONCLUSIONS
  Further investigations are necessary to improve the utility of this methodology in global metabolite profiling.
authors.affiliation: Department of Drug Metabolism & Pharmacokinetics IM, AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire, UK.
authors.forename: Filippos
authors.initials: F
authors.lastname: Michopoulos
authors.forename: Georgios
authors.initials: G
authors.lastname: Theodoridis
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Smith
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
mesh_terms: D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing; D006801:Humans; D055442:Metabolome; D010209:Paper; D025341:Principal Component Analysis; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D013997:Time Factors