title: Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy.
abstract: We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.
authors.affiliation: Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634, USA.
authors.forename: Honghai
authors.initials: H
authors.lastname: Liu
authors.forename: Wan
authors.initials: W
authors.lastname: Qin
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ma
authors.forename: Tong
authors.initials: T
authors.lastname: Ye
authors.forename: Tom
authors.initials: T
authors.lastname: Borg
authors.forename: Bruce Z
authors.initials: BZ
authors.lastname: Gao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004867:Equipment Design; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods*; D024510:Muscle Development / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012518:Sarcomeres / Q000502:physiology