title: Functional complementation of dwf4 mutants of Arabidopsis by overexpression of CYP724A1.
abstract: An essential step in the biosynthesis of bioactive brassinosteroids (BRs) in plants is the hydroxylation at C-22, a reaction catalyzed by P450 enzymes of the CYP90B and CYP724B subfamilies. Genes for both types of enzymes are present in many species, and in rice (Oryza sativa) and tomato (Solanum lycopersicum) both CYP90B and CYP724B enzymes contribute to C-22 hydroxylation. In Arabidopsis (Arabidopsis thaliana), C-22 hydroxylation of BRs is catalyzed by CYP90B1 (encoded by DWF4) and null dwf4 mutants show severe symptoms of BR-deficiency. CYP724A1 (At5g14400), an Arabidopsis gene of unknown function and limited expression, encodes a P450 sharing less than 55% sequence identity to CYP724B proteins. We used transgenic plants of the null mutants dwf4-102 and a novel allele, bashful (bsf), ectopically expressing the CYP724A1 gene to investigate the potential activity of CYP724A1 as a C-22 hydroxylase of BRs. Defects associated with BR deficiency were reversed and a normal growth habit restored in transgenic dwf4-102 and bsf plants overexpressing CYP724A1. The vegetative phase was prolonged and the transgenic plants were on average larger than wild type plants with respect to several morphometric parameters. Fertility was restored in the transgenic plants but individual siliques yielded fewer and heavier seeds than those of wild type plants. The implications of these findings with regard to the functions of CYP724A1 and the activity of its encoded enzyme are discussed.
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropic Agriculture, Chinese Academy of Sciences, Mapoling of Changsha City, Changsha, Hunan 410125, PR China.
authors.forename: Rujia
authors.initials: R
authors.lastname: Zhang
authors.forename: Xinjie
authors.initials: X
authors.lastname: Xia
authors.forename: Keith
authors.initials: K
authors.lastname: Lindsey
authors.forename: Pedro S C Ferreira
authors.initials: PS
authors.lastname: da Rocha
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018389:Codon, Nonsense; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017354:Point Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: C111451:AT3G50660 protein, Arabidopsis; D029681:Arabidopsis Proteins; C572211:CYP724A1 protein, Arabidopsis; D018389:Codon, Nonsense; D003577:Cytochrome P-450 Enzyme System