title: Development of multiplex assay with 16 SNPs on X chromosome for degraded samples.
abstract: We selected 16 new X chromosomal SNPs (rs4827155, rs471205, rs7884160, rs16982419, rs985251, rs3813932, rs6630351, rs4132871, rs5966270, rs7471388, rs6641116, rs6521038, rs5990560, rs5959408, rs414960, and rs3006142) and developed the two X chromosomal SNPs Octaplex systems using multiplex single base extension reactions. To make the systems more useful for analyzing degraded DNA samples, we designed primers to render amplicons of 100 bp or shorter (shorter PCR products). Statistical analyses of the 16 SNPs indicated a high usefulness for the Japanese forensic practice. In addition, results of tests on degraded DNA confirm the usefulness of this technique in such samples.
authors.affiliation: Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Takahito
authors.initials: T
authors.lastname: Oki
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Masao
authors.initials: M
authors.lastname: Ota
authors.forename: Hideki
authors.initials: H
authors.lastname: Asamura
mesh_terms: D041321:Chromosomes, Human, X; D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D008099:Liver / Q000473:pathology; D008297:Male; D018895:Microsatellite Repeats; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D013997:Time Factors
chemical_list: D017931:DNA Primers; D005404:Fixatives; D005557:Formaldehyde