title: Molecular cytogenetic aberrations in Tunisian patients with multiple myeloma identified by cIg-FISH in fixed bone marrow cells.
abstract: Cytogenetic studies in multiple myeloma (MM) are hampered by the hypo-proliferative nature of plasma cells. In order to circumvent this problem, we have used a combination of immunolabeling of cytoplasmic Ig light chains (λ or κ) and FISH (cIg-FISH), which allowed a comprehensive detection of the most common and/or recurrent molecular cytogenetic aberrations on fixed bone marrow cells of 70 Tunisian patients. Translocations involving the chromosome 14q32 region were observed in 32 cases (45.7%), including 18 cases with a t(11;14), 8 cases with a t(4;14), and 2 cases with a t(14;16). Deletions of the 13q14 region (D13S319/RB1) were detected in 18.6%, and deletions of the 17p13 region (TP53) in 5.7% of the cases, respectively. Of all patients with a D13S319/RB1 deletion, 61.5% also carried a 14q32 translocation, whereas TP53 deletions were associated with a t(11;14) in 2 cases (50%) and a D13S319 deletion in 1 case (25%). Our results suggest that there is a correlation between the presence of 14q32 translocations and chromosome 13q14 deletions in MM patients and that cIg-FISH is more sensitive as compared to conventional karyotyping in detecting molecular cytogenetic abnormalities in this disease.
authors.affiliation: Department of Cytogenetics and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia. gmidene_abir@yahoo.fr
authors.forename: A
authors.initials: A
authors.lastname: Gmidène
authors.forename: H
authors.initials: H
authors.lastname: Avet-Loiseau
authors.forename: H
authors.initials: H
authors.lastname: Sennana
authors.forename: I
authors.initials: I
authors.lastname: Ben Abdallah
authors.forename: A
authors.initials: A
authors.lastname: Khlif
authors.forename: B
authors.initials: B
authors.lastname: Meddeb
authors.forename: M
authors.initials: M
authors.lastname: Elloumi
authors.forename: A
authors.initials: A
authors.lastname: Saad
mesh_terms: D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000648:ultrastructure*; D002648:Child; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D002883:Chromosomes, Human, Pair 14; D020732:Cytogenetic Analysis / Q000379:methods; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping / Q000379:methods; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism; D014178:Translocation, Genetic; D014416:Tunisia
chemical_list: D007143:Immunoglobulin Heavy Chains