title: Label-free Raman observation of cytochrome c dynamics during apoptosis.
abstract: We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.
authors.affiliation: Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Okada
authors.forename: Nicholas Isaac
authors.initials: NI
authors.lastname: Smith
authors.forename: Almar Flotildes
authors.initials: AF
authors.lastname: Palonpon
authors.forename: Hiromi
authors.initials: H
authors.lastname: Endo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawata
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sodeoka
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Fujita
mesh_terms: D017209:Apoptosis; D045304:Cytochromes c / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D012996:Solutions; D013059:Spectrum Analysis, Raman; D013194:Staining and Labeling; D059008:Time-Lapse Imaging
chemical_list: D012996:Solutions; D045304:Cytochromes c