title: Cloning of hemoglobin-α1 from half-smooth tongue sole (Cynoglossus semilaevis) and its expression under short-term hypoxia.
abstract: This study cloned the hemoglobin α1 from the marine teleost, the half-smooth tongue sole (Cynoglossus semilaevis), and then examined its expression under hypoxia exposure. The full-length of CsHb-α1 (594 bp) cDNA contains an open reading frame encoding 144 amino acids. Sequence analysis shows that the predicted CsHb-α1 amino acids shares high identities with that of other species. Real-time PCR showed that CsHb-α1 was highly expressed in the heart, liver, spleen, kidney and blood. Five to 120 min esposure and long-term (36 h) exposure to hypoxia (1.0 mg/L) significantly increased CsHb-α1 mRNA expression in most tissues compared to those fish held in normoxic conditions (dissolved oxygen (DO): 6.2 mg/L). These results suggested that the up-regulation of Hb-α1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.
authors.affiliation: Key Laboratory of Aquaculture and Ecology of Coastal Pool in Jiangsu Province, Department of Ocean Technology, Yancheng Institute of Technology, Yancheng Jiangsu 224051, China. wzs@ycit.cn
authors.forename: Zi-Sheng
authors.initials: ZS
authors.lastname: Wang
authors.forename: Zhi-Tao
authors.initials: ZT
authors.lastname: Qi
authors.forename: Jing-Yun
authors.initials: JY
authors.lastname: Tian
authors.forename: Ming
authors.initials: M
authors.lastname: Qiu
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Zhao
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Wang
authors.forename: Jin-Tian
authors.initials: JT
authors.lastname: Huang
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D029941:Fish Proteins; D006454:Hemoglobins; D010100:Oxygen