title: Effect of suture properties on stability of middle cerebral artery occlusion evaluated by synchrotron radiation angiography.
abstract: OBJECTIVE
  The intraluminal suture technique for producing middle cerebral artery occlusion in rodents is the most commonly used method for modeling focal cerebral ischemia associated with clinical ischemic stroke. Synchrotron radiation angiography may provide a novel solution to directly monitor the success of middle cerebral artery occlusion.
  
  
  METHODS
  Twenty adult Sprague-Dawley rats for middle cerebral artery occlusion models were prepared randomly with different suture head silicone coating. In vivo imaging was performed at beam line BL13W1, Shanghai Synchrotron Radiation Facility, Shanghai, China.
  
  
  RESULTS
  Silicone-coated suture was superior to uncoated suture for producing consistent brain infarction. Additionally, silicone coating length was an important variable controlling the extent of the ischemic lesion: infarcts affected predominantly the caudate-putamen with large variability (<2 mm), both the cortex and caudate-putamen (2-3.3 mm), and most of the hemisphere, including the hypothalamus (>3.3 mm).
  
  
  CONCLUSIONS
  Synchrotron radiation angiography provides a useful tool to observe hemodynamic changes after middle cerebral artery occlusion, and the physical properties of suture are critical to the success of the middle cerebral artery occlusion model.
authors.affiliation: Department of Radiology, Ruijin Hospital, School of Medicine, Shanghai, China.
authors.forename: Yongjing
authors.initials: Y
authors.lastname: Guan
authors.forename: Yongting
authors.initials: Y
authors.lastname: Wang
authors.forename: Falei
authors.initials: F
authors.lastname: Yuan
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lu
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Ren
authors.forename: Tiqiao
authors.initials: T
authors.lastname: Xiao
authors.forename: Kemin
authors.initials: K
authors.lastname: Chen
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
authors.forename: Kunlin
authors.initials: K
authors.lastname: Jin
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging; D002545:Brain Ischemia / Q000209:etiology / Q000503:physiopathology; D002421:Caudate Nucleus / Q000000981:diagnostic imaging; D002533:Cerebral Angiography / Q000379:methods*; D002540:Cerebral Cortex / Q000000981:diagnostic imaging; D002560:Cerebrovascular Circulation / Q000502:physiology; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000502:physiology; D020244:Infarction, Middle Cerebral Artery / Q000000981:diagnostic imaging* / Q000209:etiology; D008026:Ligation; D008297:Male; D020768:Middle Cerebral Artery / Q000000981:diagnostic imaging*; D011699:Putamen / Q000000981:diagnostic imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones; D020521:Stroke / Q000209:etiology / Q000503:physiopathology; D013537:Sutures; D017356:Synchrotrons
chemical_list: D012828:Silicones