title: The oxidative effect of prolonged CO(2) pneumoperitoneum a comparative study in rats.
abstract: BACKGROUND
  The current study evaluated the effect of time in the severity of the oxidative stress due to pneumoperitoneum.
  
  
  METHODS
  Forty Wistar rats were allocated randomly into 2 groups. The 1 h pneumoperitoneum (Pp) group, which was subjected to 60 min of pneumoperitoneum, and the 3 h Pp, to pneumoperitoneum for 180 min. The animals were divided in half. One half of the rats were left resting for 30 min after abdominal desufflation and the other for 8 h. After these two time periods, blood, liver, kidney, lung and small intestine were obtained for biochemical analysis and histopathological examination.
  
  
  RESULTS
  In the 3 h Pp, the associated oxidative stress was increased. There was an overt increase in blood and tissue MDA and blood PAB values. The MPO values were significantly higher in the 3 h Pp group in serum, kidneys, and intestine during the early phase of reperfusion and in liver after 8 h of reperfusion. These changes occurred in the presence of light microscopic evidence of greater tissue damage for the 3 h Pp, which were consistent with the fluctuation of the MPO values.
  
  
  CONCLUSIONS
  In our experimental model, we proved biochemically and histologically that time of maintenance of pneumoperitoneum is an additive factor that could cause increased oxidative stress in laparoscopic procedures.
authors.affiliation: Second Propaedeutical Department of Surgery, Medical School, Aristotle University of Thessaloniki, Hippocration Hospital, Thessaloniki, Greece. tedkondoulis@hotmail.com
authors.forename: Theodoros M
authors.initials: TM
authors.lastname: Kontoulis
authors.forename: Dimitrios G
authors.initials: DG
authors.lastname: Pissas
authors.forename: Theodoros E
authors.initials: TE
authors.lastname: Pavlidis
authors.forename: Georgios G
authors.initials: GG
authors.lastname: Pissas
authors.forename: Miltiadis A
authors.initials: MA
authors.lastname: Lalountas
authors.forename: Georgios
authors.initials: G
authors.lastname: Koliakos
authors.forename: Konstantina
authors.initials: K
authors.lastname: Topouridou
authors.forename: Athanasios K
authors.initials: AK
authors.lastname: Sakantamis
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002245:Carbon Dioxide / Q000008:administration & dosage*; D016207:Cytokines / Q000097:blood*; D007263:Infusions, Parenteral; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D023421:Models, Animal; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015415:Biomarkers; D016207:Cytokines; D002245:Carbon Dioxide; D008315:Malondialdehyde; D009195:Peroxidase