title: Cisplatin effects on guinea pigs: cochlear histology and genotoxicity.
abstract: To understand how the DNA answers to external agents such as cisplatin may be relevant to the diagnosis and treatment of hearing disorders caused by the administration of such drug.
  
  
  OBJECTIVE
  To investigate the cisplatin influence on the cochlea and DNA of guinea pigs.
  
  
  METHODS
  Experimental study carried out with 12 guinea pigs (Cavia porcellus). The inclusion criterion was the presence of Preyer's reflex and distortion-product otoacoustic emissions. Guinea pigs were divided into two groups: Control Group (CG)--made up of six guinea pigs, to which we administrated saline solution during six consecutive days, intraperitoneally; and a Study Group (SG)--made up of six guinea pigs, to which we administrated cisplatin during six consecutive doses of 3mg/kg/day intraperitoneally. Twenty-four hours after the last administration of cisplatin the guinea pigs were slaughtered, blood samples were collected and the cochleae were removed.
  
  
  RESULTS
  The administration of cisplatin did not cause identifiable changes to the DNA. Histological analysis showed changes in the organ of Corti and spiral ganglion.
  
  
  CONCLUSIONS
  Cisplatin causes changes in cochlear histology, such as the loss of the normal micro-cytoarchitecture of the organ of Corti, and reduction of neurons of the spiral ganglion with cell alterations, however, DNA damage was not detected.
authors.affiliation: Curso de Fonoaudiologia Programa de Pós-Graduação em Distúrbios da Comunicação Humana, Universidade Federal de Santa Maria.
authors.forename: Cacineli Marion de
authors.initials: CM
authors.lastname: Franceschi
authors.forename: Tania
authors.initials: T
authors.lastname: Tochetto
authors.forename: Aron Ferreira da
authors.initials: AF
authors.lastname: Silveira
authors.forename: Mara Rejane
authors.initials: MR
authors.lastname: Fantinel
authors.forename: Thaís Doeler
authors.initials: TD
authors.lastname: Algarve
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects* / Q000473:pathology; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D006168:Guinea Pigs; D009152:Mutagenicity Tests
chemical_list: D000970:Antineoplastic Agents; D004247:DNA; D002945:Cisplatin