title: Immunohistochemical characterization of wound healing at two different bone graft substitutes.
abstract: The immunohistochemical characteristics of wound healing following application of a biphasic calcium phosphate or a collagen coated natural bone combined with a native collagen membrane in a dog model was assessed. Standardized buccal dehiscence-type defects were surgically created following implant bed preparation in 6 dogs. Following implant placement, defects were randomly filled with a collagen coated natural bone mineral (GBO), or a biphasic hydroxyapatite/beta tricalcium phosphate (SBC), and covered with a native collagen membrane. After 1, 4, and 9 weeks' submerged healing, dissected blocks were processed for immunohistochemical (collagen type I (CI), osteocalcin (OC), angiogenesis (TG)) analysis. At 1 week, GBO and SBC granules were homogeneously surrounded by a well vascularized, non-mineralized tissue (NMT). CI and OC antigen reactivity was commonly observed adjacent to both bone graft substitutes. At 4 and 9 weeks, SBC and GBO granules were completely integrated into a secondly formed network of spongiosa. At 9 weeks, dissolution of some granules was observed in the SBC group. Adjacent to these granules, NMT was significantly increased and revealed a pronounced CI, OC and TG antigen reactivity. The initial pattern of bone regeneration and graft integration was comparable in both groups; bone remodelling was more pronounced with SBC.
authors.affiliation: Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sager
authors.forename: D
authors.initials: D
authors.lastname: Ferrari
authors.forename: M
authors.initials: M
authors.lastname: Wieland
authors.forename: M
authors.initials: M
authors.lastname: Dard
authors.forename: J
authors.initials: J
authors.lastname: Becker
authors.forename: F
authors.initials: F
authors.lastname: Schwarz
mesh_terms: D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000601:surgery; D000540:Alveolar Ridge Augmentation / Q000379:methods; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D001861:Bone Regeneration / Q000502:physiology; D016723:Bone Remodeling / Q000502:physiology; D018786:Bone Substitutes / Q000627:therapeutic use*; D003094:Collagen / Q000627:therapeutic use*; D024042:Collagen Type I / Q000032:analysis; D015921:Dental Implants; D004195:Disease Models, Animal; D004285:Dogs; D006882:Hydroxyapatites / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008437:Maxilla / Q000473:pathology; D008439:Maxillary Diseases / Q000601:surgery; D008567:Membranes, Artificial; D008903:Minerals / Q000627:therapeutic use*; D018919:Neovascularization, Physiologic / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology; D011897:Random Allocation; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology
chemical_list: C106416:Bio-Gide; C077540:Bio-Oss; D018786:Bone Substitutes; D024042:Collagen Type I; D015921:Dental Implants; D006882:Hydroxyapatites; D008567:Membranes, Artificial; D008903:Minerals; C074950:hydroxyapatite-beta tricalcium phosphate; D015675:Osteocalcin; D003094:Collagen