title: Adenosine deaminase activity in serum and lymphocytes of rats infected with Sporothrix schenckii.
abstract: Sporotrichosis is a fungal infection of subcutaneous or chronic evolution, inflammatory lesions characterized by their pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a "key" enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions; however, there are no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and L-ADA were performed at days 15, 30, and 40 post-infection (PI) to assess disease progression. In the second experiment, it was observed an acute decrease in activity of S-ADA and L-ADA (P < 0.05), suggesting a compensatory mechanism in an attempt to protect the host from excessive tissue damage. With chronicity of disease the rats in the first and second experiment at 30 days PI showed an increased activity of L-ADA (P < 0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA in experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis.
authors.affiliation: Department of Small Animals, Federal University of Santa Maria, Camobi, 9, Veterinary Hospital, Room 109, Santa Maria, RS, 97105900, Brazil. castrove@gmail.com
authors.forename: Verônica S P
authors.initials: VS
authors.lastname: Castro
authors.forename: Victor C
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Gustavo R
authors.initials: GR
authors.lastname: Thomé
authors.forename: Patrícia
authors.initials: P
authors.lastname: Wolkmer
authors.forename: Jorge L C
authors.initials: JL
authors.lastname: Castro
authors.forename: Márcio M
authors.initials: MM
authors.lastname: Costa
authors.forename: Cássia B
authors.initials: CB
authors.lastname: da Silva
authors.forename: Daniele C
authors.initials: DC
authors.lastname: Oliveira
authors.forename: Sydney H
authors.initials: SH
authors.lastname: Alves
authors.forename: Maria R C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Sonia T A
authors.initials: ST
authors.lastname: Lopes
authors.forename: Cinthia M
authors.initials: CM
authors.lastname: Mazzanti
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D054884:Host-Pathogen Interactions; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044967:Serum / Q000737:chemistry*; D013175:Sporothrix / Q000276:immunology* / Q000472:pathogenicity*; D013174:Sporotrichosis / Q000276:immunology* / Q000473:pathology*; D013997:Time Factors
chemical_list: D000243:Adenosine Deaminase