title: HPLC/UV or bioassay: two valid methods for posaconazole quantification in human serum samples.
abstract: In this paper we report on the development and validation of two different methods for posaconazole quantification from serum samples, HPLC/UV and bioassay. Both methods have been validated according to international guidelines and were also applied to the analysis of 61 trough serum samples from treated patients. A good correlation between both methods was observed. The HPLC method, more laborious and expensive, was demonstrated to be more accurate, precise and faster (analytical range 0.125-16 μg/mL, accuracy between -2.48 and 3.70% and precision between 2.77 and 5.93%, with an analytical run time of 11 min), making it a valuable tool for reference laboratories that centralize high numbers of samples. The microbiological method, however, is simple and offers sufficient precision and accuracy (analytical range 0.125-16 μg/mL, accuracy between -8.10 and 3.77% and a precision between 4.52 and 10.07%), to be used to monitor posaconazole. It may be a valid alternative to chromatographic methods in clinical laboratories without specialized facilities.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Cendejas-Bueno
authors.forename: A
authors.initials: A
authors.lastname: Forastiero
authors.forename: J L
authors.initials: JL
authors.lastname: Rodriguez-Tudela
authors.forename: M
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: A
authors.initials: A
authors.lastname: Gomez-Lopez
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D001681:Biological Assay / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008297:Male; D044967:Serum / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014230:Triazoles / Q000097:blood*
chemical_list: D000935:Antifungal Agents; D014230:Triazoles; C101425:posaconazole