title: Purification, characterizations of a snake guard seeds lectin with antitumor activity against Ehrlich ascites carcinoma cells in vivo in mice.
abstract: A lectin was purified (designated as TCSL) from the Snake guard seeds with molecular mass of 56±2 kDa containing two subunits (34±1 and 22±1 kDa.). TCSL exhibited high agglutination activity at the temperature range 30 to 70°C and did not lose its activity between pH 3.0 to 12.0. The lectin was stable in the presence of denaturants and agglutinated mouse, goat, cow, chicken and human erythrocytes. TCSL did not show antifungal activity whereas it agglutinated six pathogenic bacteria and showed less toxicity against brine shrimp nauplii with the LC50 of 261±29 μg/ml. TCSL showed 28% and 72% inhibition against Ehrlich ascites carcinoma (EAC) cells in vivo in mice when administered 1 mg/kg/day and 2 mg/kg/day (i.p.) respectively for five days. TCSL enhanced the number of macrophages remarkably in the normal mice. The lectin reduced the tumor burden to 62% of EAC cells and significantly increased the hemoglobin and RBC. Treating the EAC bearing mice with TCSL at 2 mg/Kg/day for ten days with a monitoring of 20 days decreased the total WBC towards the normal level and it increased the life span by 39%.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi-6205, Bangladesh. rashelkabir@ru.ac.bd
authors.forename: Syed Rashel
authors.initials: SR
authors.lastname: Kabir
authors.forename: Md Farhadul
authors.initials: MF
authors.lastname: Islam
authors.forename: Md Jahangir
authors.initials: MJ
authors.lastname: Alom
authors.forename: Md
authors.initials: M
authors.lastname: Abu Zubair
authors.forename: Nurul
authors.initials: N
authors.lastname: Absar
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D001419:Bacteria / Q000187:drug effects; D001789:Blood Group Antigens / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D010529:Peritoneal Cavity / Q000166:cytology; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D011489:Protein Denaturation / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D015996:Survival Rate; D013696:Temperature; D029778:Trichosanthes / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D001789:Blood Group Antigens; D037121:Plant Lectins