title: Expression of rice thaumatin-like protein gene in transgenic banana plants enhances resistance to fusarium wilt.
abstract: The possibility of controlling Fusarium wilt--caused by Fusarium oxysporum sp. cubensec (race 4)--was investigated by genetic engineering of banana plants for constitutive expression of rice thaumatin-like protein (tlp) gene. Transgene was introduced to cauliflower-like bodies' cluster, induced from meristemic parts of male inflorescences, using particle bombardment with plasmid carrying a rice tlp gene driving by the CaMV 35S promoter. Hygromycin B was used as the selection reagent. The presence and integration of rice tlp gene in genomic DNA confirmed by PCR and Southern blot analyses. RT-PCR revealed the expression of transgene in leaf and root tissues in transformants. Bioassay of transgenic banana plants challenged with Fusarium wilt pathogen showed that expression of TLP enhanced resistance to F. oxysporum sp. cubensec (race 4) compared to control plants.
authors.affiliation: Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. a.mahdavi.d@gmail.com
authors.forename: F
authors.initials: F
authors.lastname: Mahdavi
authors.forename: M
authors.initials: M
authors.lastname: Sariah
authors.forename: M
authors.initials: M
authors.lastname: Maziah
mesh_terms: D015139:Blotting, Southern; D005670:Fusarium / Q000472:pathogenicity / Q000502:physiology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D006921:Hygromycin B; D028521:Musa / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D012275:Oryza; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D018515:Plant Leaves / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*; D018517:Plant Roots / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019076:Transgenes
chemical_list: D010940:Plant Proteins; D006921:Hygromycin B; C003427:thaumatin protein, plant