title: First microscopical and molecular-based characterization of Leishmania major within naturally infected Phlebotomus salehi (Diptera; Psychodidae) in Fars province, southern Iran.
abstract: Zoonotoc cutaneous leishmaniasis is endemic in several parts of Iran. Jahrom district is one of the most important endemic foci of leishmaniasis located in Fars province, southern Iran. To identify the vectors of leishmaniasis in this area, a total of 349 sandflies were collected during May to August 2009. They were caught from outdoors in five regions of Jahrom district including villages of Mousavieh, Ghotb-Abad, Heydar-Abad, Fath-Abad and Jahrom County. Eleven species of Phlebotomine (three Phlebotomus spp. and eight Sergentomyia spp.) were detected. To determine the sandflies naturally infected by Leishmania spp., 122 female sandflies were dissected and evaluated microscopically using Giemsa-stained slides. Natural infection of 2 out of 38 (5.26%) P. papatasi and 1 out of 8 (12.5%) P. salehi to Leishmania major was confirmed in the region. Sequencing and nested polymerase chain reaction-based detection of Leishmania were carried out to confirm the microscopic findings. Five (13.16%) P. papatasi and two (25%) P. salehi were positive in nested polymerase chain reaction assay. All positive samples were shown 72-76% similarity with L. major Friedlin. On the basis of our knowledge, this is the first molecular detection of L. major within naturally infected P. salehi in this region in southern Iran.
authors.affiliation: Department of Medical Microbiology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: M H
authors.initials: MH
authors.lastname: Davami
authors.forename: M H
authors.initials: MH
authors.lastname: Motazedian
authors.forename: M
authors.initials: M
authors.lastname: Kalantari
authors.forename: Q
authors.initials: Q
authors.lastname: Asgari
authors.forename: A
authors.initials: A
authors.lastname: Badzohre
authors.forename: I
authors.initials: I
authors.lastname: Mohammadpour
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000302:isolation & purification; D004175:Diptera / Q000472:pathogenicity; D004197:Disease Reservoirs; D005260:Female; D006801:Humans; D007492:Iran / Q000453:epidemiology; D018320:Leishmania major / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000235:genetics*; D008853:Microscopy; D010691:Phlebotomus / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D011576:Psychodidae / Q000472:pathogenicity; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan