title: Diversity array technology markers: genetic diversity analyses and linkage map construction in rapeseed (Brassica napus L.).
abstract: We developed Diversity Array Technology (DArT) markers for application in genetic studies of Brassica napus and other Brassica species with A or C genomes. Genomic representation from 107 diverse genotypes of B. napus L. var. oleifera (rapeseed, AACC genomes) and B. rapa (AA genome) was used to develop a DArT array comprising 11 520 clones generated using PstI/BanII and PstI/BstN1 complexity reduction methods. In total, 1547 polymorphic DArT markers of high technical quality were identified and used to assess molecular diversity among 89 accessions of B. napus, B. rapa, B. juncea, and B. carinata collected from different parts of the world. Hierarchical cluster and principal component analyses based on genetic distance matrices identified distinct populations clustering mainly according to their origin/pedigrees. DArT markers were also mapped in a new doubled haploid population comprising 131 lines from a cross between spring rapeseed lines 'Lynx-037DH' and 'Monty-028DH'. Linkage groups were assigned on the basis of previously mapped simple sequence repeat (SSRs), intron polymorphism (IP), and gene-based markers. The map consisted of 437 DArT, 135 SSR, 6 IP, and 6 gene-based markers and spanned 2288 cM. Our results demonstrate that DArT markers are suitable for genetic diversity analysis and linkage map construction in rapeseed.
authors.affiliation: EH Graham Centre for Agricultural Innovation, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW 2650, Australia. harsh.raman@industry.nsw.gov.au
authors.forename: Harsh
authors.initials: H
authors.lastname: Raman
authors.forename: Rosy
authors.initials: R
authors.lastname: Raman
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Nelson
authors.forename: M N
authors.initials: MN
authors.lastname: Aslam
authors.forename: Ravikesavan
authors.initials: R
authors.lastname: Rajasekaran
authors.forename: Neil
authors.initials: N
authors.lastname: Wratten
authors.forename: Wallace A
authors.initials: WA
authors.lastname: Cowling
authors.forename: A
authors.initials: A
authors.lastname: Kilian
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Sharpe
authors.forename: Joerg
authors.initials: J
authors.lastname: Schondelmaier
mesh_terms: D029688:Brassica napus / Q000145:classification / Q000235:genetics*; D029741:Brassica rapa / Q000145:classification / Q000235:genetics*; D056915:DNA Copy Number Variations; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers