title: Genome-wide approaches to determining nucleosome occupancy in metazoans using MNase-Seq.
abstract: The precise location of nucleosomes in functional regulatory regions in chromatin is critical to the regulation of transcription. The nucleosome structure protects DNA from microccocal nuclease (MNase) digestion and leaves a footprint on DNA that indicates the position of nucleosomes. Short sequence reads (25-36 bp) from ends of mononucleosome-sized DNA generated from MNase digestion of chromatin can be determined using next-generation sequencing techniques. Mapping of these short reads to the genome provides a powerful genome-wide approach to precisely define the nucleosome positions in any genome with known genomic sequence. This chapter outlines the reagents and experimental procedures of MNase-Seq for mapping nucleosome positions in the human genome.
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Kairong
authors.initials: K
authors.lastname: Cui
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D016678:Genome / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006801:Humans; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013223:Statistics as Topic; D013698:Templates, Genetic
chemical_list: D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease