title: PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging.
abstract: We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe(3)O(4)), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe(3)O(4)-PEG-FITC-CNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe(3)O(4) nanoparticles were well anchored onto the surfaces of the CNT. In vitro time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe(3)O(4)-PEG-FITC-CNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with in vitro non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe(3)O(4)-PEG-FITC-CNT nanosystem an ideal candidate for bioimaging, both in vitro and in vivo.
authors.affiliation: NCE-Polymer Chemistry Group, Piramal Life Sciences Ltd., Goregaon, Mumbai-400063, India.
authors.forename: Jayant J
authors.initials: JJ
authors.lastname: Khandare
authors.forename: Archana
authors.initials: A
authors.lastname: Jalota-Badhwar
authors.forename: Sneha D
authors.initials: SD
authors.lastname: Satavalekar
authors.forename: Sujit G
authors.initials: SG
authors.lastname: Bhansali
authors.forename: Naval D
authors.initials: ND
authors.lastname: Aher
authors.forename: Firuza
authors.initials: F
authors.lastname: Kharas
authors.forename: Shashwat S
authors.initials: SS
authors.lastname: Banerjee
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D003952:Diagnostic Imaging / Q000379:methods; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D055620:Optical Phenomena; D011092:Polyethylene Glycols
chemical_list: D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate