title: Study on the interaction between methyl jasmonate and the coiled-coil domain of rice blast resistance protein Pi36 by spectroscopic methods.
abstract: Interaction between the coiled-coil domain of rice blast resistance protein Pi36 and methyl-jasmonate (MeJA) was studied by fluorescence and UV-vis spectroscopic techniques. The quenching mechanism of fluorescence of MeJA by this domain was discussed to be a static quenching procedure. Fluorescence quenching was explored to measure the number of binding sites n and apparent binding constants K. The thermodynamics parameters ΔH, ΔG, ΔS were also calculated. The results indicate the binding reaction was not entropy-driven but enthalpy-driven, and hydrophobic binding played major role in the interaction. The binding sites of MeJA with the coiled-coil structural domain of rice blast resistance protein Pi36 were found to approach the microenvironment of both Tyr and Trp by the synchronous fluorescence spectrometry. The distance r between donor (the coiled-coil domain of rice blast resistance protein Pi36) and acceptor (MeJA) was obtained according to Förster theory of non-radioactive energy transfer.
authors.affiliation: Key Biotechnology Laboratory of State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan, China.
authors.forename: Xin Q
authors.initials: XQ
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiang M
authors.initials: XM
authors.lastname: Zhang
authors.forename: Chun T
authors.initials: CT
authors.lastname: Wang
authors.forename: Xue Q
authors.initials: XQ
authors.lastname: Liu
authors.forename: Yan P
authors.initials: YP
authors.lastname: Tan
authors.forename: Yun H
authors.initials: YH
authors.lastname: Wu
mesh_terms: D000042:Absorption; D000085:Acetates / Q000378:metabolism*; D001665:Binding Sites; D003517:Cyclopentanes / Q000378:metabolism*; D060467:Disease Resistance; D007700:Kinetics; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate