title: Validation and application of RP-HPLC method for the quantification of metoclopramide hydrochloride in oral formulations prepared for IVIVC studies.
abstract: The objective of this study is to develop sensitive and cost effective reverse phase high performance liquid chromatographic method for the estimation of Metoclopramide Hydrochloride in oral solid dosage formulations. A reverse chromatographic method was used with the mobile phase of Acetonitrile, 20m M Potassium dihydrogen phosphate buffer solution (pH 3 adjusted with orthophosphoric acid) in the ratio of 40:60. The column used was Waters C18 3.9×300mm µBondapak (RP). The flow rate of the mobile phase was 2ml/minute. The detector was set at the wavelength of 275nm.This method showed good sensitivity. The linearity was also found to be excellent (γ(2)=0.997) in the range of 5-75 μg/ml. No interfering peaks were observed at the retention time of Metoclopramide Hydrochloride when both placebo and blank samples were injected (Retention time =1.93min). The parameters such as specificity, linearity,   range,   accuracy,   precision,  system  suitability,  solution stability,  detection  and  quantification  limits were  evaluated  to validate  this method.  This method can effectively be used for quantitative analysis of Metoclopramide hydrochloride tablet formulations because of its specificity, accuracy and convenience of use.
authors.affiliation: Department of Pharmaceutics, University of Karachi, Pakistan. ahmadkingsk@yahoo.com
authors.forename: Ahmad
authors.initials: A
authors.lastname: Khan
authors.forename: Syed Baqir S
authors.initials: SB
authors.lastname: Naqvi
authors.forename: Muhammad Harris
authors.initials: MH
authors.lastname: Shoaib
authors.forename: Rabia Ismail
authors.initials: RI
authors.lastname: Yousaf
authors.forename: Jallat
authors.initials: J
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Hanif
authors.forename: Asadullah
authors.initials: A
authors.lastname: Madni
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods* / Q000706:statistics & numerical data; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004355:Drug Stability; D057230:Limit of Detection; D008787:Metoclopramide / Q000032:analysis*; D013607:Tablets / Q000737:chemistry*
chemical_list: D013607:Tablets; D008787:Metoclopramide