title: Traditional medicine alpinetin inhibits the inflammatory response in Raw 264.7 cells and mouse models.
abstract: Alpinetin, one of the main constituents of the seeds of Alpinia katsumadai Hayata, belonging to flavonoids, has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The purpose of this study was to investigate the protection of alpinetin on inflammation in Lipopolysaccharide (LPS) stimulated Raw 264.7 cells and LPS induced vivo lung injury model. The effects of alpinetin on pro-inflammatory cytokines and signaling pathways were analyzed by enzyme-linked immunosorbent assay and Western blot. The results showed that alpinetin markedly inhibited the LPS- induced TNF-α, IL-6 and IL-1β production both in vitro and vivo. Furthermore, alpinetin blocked the phosphorylation of IκBα protein, p65, p38 and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells. From in vivo study, it was also observed that alpinetin attenuated lung histopathologic changes in mouse models. These results suggest that alpinetin potentially decreases the inflammation in vitro and vivo, and might be a therapeutic agent against inflammatory diseases.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Meixia
authors.initials: M
authors.lastname: Huo
authors.forename: Na
authors.initials: N
authors.lastname: Chen
authors.forename: Gefu
authors.initials: G
authors.lastname: Chi
authors.forename: Xue
authors.initials: X
authors.lastname: Yuan
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.forename: Weiting
authors.initials: W
authors.lastname: Zhong
authors.forename: Weixiao
authors.initials: W
authors.lastname: Guo
authors.forename: Lanan Wassy
authors.initials: LW
authors.lastname: Soromou
authors.forename: Ruijie
authors.initials: R
authors.lastname: Gao
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.forename: Haihua
authors.initials: H
authors.lastname: Feng
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D044950:Flavanones / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000276:immunology; D016328:NF-kappa B / Q000276:immunology; D008517:Phytotherapy
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D008070:Lipopolysaccharides; D016328:NF-kappa B; C436748:alpinetin; D020928:Mitogen-Activated Protein Kinases