title: Role of substrates in diabetes therapy: stem cell differentiation and islet transplantation.
abstract: Type 1 diabetes affects more than a million people in the United States and many more across the world. While pharmaceutical interventions and insulin supplementation are the most commonplace treatment of diabetes, these are not essentially cures and can potentially lead to long-term complications. Transplantation of insulin-producing Islets of Langerhans from donor pancreas has been established as a promising alternative to diabetes therapy. While successful islet transplantation has the potential of providing a cure, the primary hurdles to be overcome for it to be clinically viable are the scarcity of donor islets and immune rejection of transplanted islets. Recent advances in stem cell culture and differentiation techniques have established stem cells as a likely source of transplantable islets. Different stem cell sources have been induced toward pancreatic differentiation using specific chemical perturbations along with use of specific substrates. An approach to overcoming the second hurdle of immune rejection of transplantable islets is to encapsulate the islets in specific biomaterials. In this review, we discuss the extensive use of various substrates for pancreatic differentiation of different stem cell sources, along with different biomaterial designs used for islet transplantation.
authors.affiliation: Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Candiello
authors.forename: Maria
authors.initials: M
authors.lastname: Jaramillo
authors.forename: Saik-Kia
authors.initials: SK
authors.lastname: Goh
authors.forename: Ipsita
authors.initials: I
authors.lastname: Banerjee
mesh_terms: D000464:Alginates / Q000627:therapeutic use; D002454:Cell Differentiation; D003094:Collagen / Q000627:therapeutic use; D003920:Diabetes Mellitus / Q000628:therapy*; D004337:Drug Carriers / Q000627:therapeutic use; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000627:therapeutic use; D006084:Graft Rejection / Q000517:prevention & control; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology / Q000601:surgery; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D007797:Laminin / Q000627:therapeutic use; D010179:Pancreas / Q000166:cytology / Q000196:embryology; D011509:Proteoglycans / Q000627:therapeutic use; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology
chemical_list: D000464:Alginates; D004337:Drug Carriers; D004338:Drug Combinations; D005353:Fibronectins; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen