title: IgG purification by bentonite-acrylamide-histidine microcomposite.
abstract: In this work, a new microcomposite composed of bentonite, acrylamide and histidine, as a pseudospecific ligand, was synthesized by bulk polymerization. The aim of this study was to improve IgG adsorption capacity of bentonite by incorporating histidine. The surface areas of the bentonite and bentonite-acrylamide-histidine microcomposites were 33.4 and 1.42 m(2)/g, respectively. The amount of histidine was found to be 50 μmol/g bentonite via elemental analysis. Adsorption capacity was at the value of 100mg/g from aqueous solution while adsorption capacity was 108 mg/g from human plasma with a purity of 90%. IgG biomolecules were able to be adsorbed and desorbed five times by using the same microcomposites without significant loss in their adsorption capacity.
authors.affiliation: Cumhuriyet University, Faculty of Science, Department of Molecular Biology and Genetics, Sivas, Turkey. pergamonchem@gmail.com
authors.forename: Birnur
authors.initials: B
authors.lastname: Akkaya
mesh_terms: D020106:Acrylamide / Q000737:chemistry*; D000327:Adsorption; D001546:Bentonite / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D059027:Recycling; D012996:Solutions; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D007074:Immunoglobulin G; D012996:Solutions; D001546:Bentonite; D020106:Acrylamide; D006639:Histidine