title: Protein profile changes during porcine oocyte aging and effects of caffeine on protein expression patterns.
abstract: It has been shown that oocyte aging critically affects reproduction and development. By using proteomic tools, in the present study, changes in protein profiles during porcine oocyte aging and effects of caffeine on oocyte aging were investigated. By comparing control MII oocytes with aging MII oocytes, we identified 23 proteins that were up-regulated and 3 proteins that were down-regulated during the aging process. In caffeine-treated oocytes, 6 proteins were identified as up-regulated and 12 proteins were identified as down-regulated. A total of 38 differentially expressed proteins grouped into 5 regulation patterns were determined to relate to the aging and anti-aging process. By using the Gene Ontology system, we found that numerous functional gene products involved in metabolism, stress response, reactive oxygen species and cell cycle regulation were differentially expressed during the oocyte aging process, and most of these proteins are for the first time reported in our study, including 2 novel proteins. In addition, several proteins were found to be modified during oocyte aging. These data contribute new information that may be useful for future research on cellular aging and for improvement of oocyte quality.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Guang-Jian
authors.initials: GJ
authors.lastname: Jiang
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Miao
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002110:Caffeine / Q000494:pharmacology*; D016922:Cellular Senescence / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results; D034421:Sus scrofa / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020543:Proteome; D002110:Caffeine