title: Bacterial magnetic particles as a novel and efficient gene vaccine delivery system.
abstract: DNA vaccination is an attractive approach for eliciting antigen-specific immunity. In this study, we used magnetosomes (bacterial magnetic particles, BMPs) as carriers of a recombinant DNA composed of a secondary lymphoid tissue chemokine, human papillomavirus type E7 (HPV-E7) and Ig-Fc fragment (pSLC-E7-Fc) to generate a gene vaccine (BMP-V) for tumour immunotherapy. The results indicate that BMPs linked to DNA more efficiently in phosphate-buffered saline (pH=4-5) than in physiological saline. Efficient transfection of BMP-V in vitro and in vivo was achieved when a 600-mT static magnetic field was applied for 10 min. In a mouse tumour model, subcutaneous injection of BMP-V (5 μg, × 3 at 4-day intervals) plus magnetic exposure elicited systemic HPV-E7-specific immunity leading to significant tumour inhibition. The treated mice tolerated BMP-V immunisation well with no toxic side effects, as shown by histopathological examinations of major internal organs. Taken together, these results suggest that BMP can be used as a gene carrier to elicit a systemic immune response.
authors.affiliation: Department of Immunology, Cancer Institute and Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Y-S
authors.initials: YS
authors.lastname: Tang
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Zhou
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D018014:Gene Transfer Techniques; D007128:Immunoglobulin Fragments; D055944:Magnetosomes; D008546:Melanoma, Experimental / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D027383:Papillomaviridae / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
chemical_list: C581244:BMP-PEI-pSLC-E7-Fc vaccine; D007128:Immunoglobulin Fragments; D019444:Vaccines, DNA