title: Synchrotron radiation X-ray microfluorescence reveals polarized distribution of atomic elements during differentiation of pluripotent stem cells.
abstract: The mechanisms underlying pluripotency and differentiation in embryonic and reprogrammed stem cells are unclear. In this work, we characterized the pluripotent state towards neural differentiated state through analysis of trace elements distribution using the Synchrotron Radiation X-ray Fluorescence Spectroscopy. Naive and neural-stimulated embryoid bodies (EB) derived from embryonic and induced pluripotent stem (ES and iPS) cells were irradiated with a spatial resolution of 20 µm to make elemental maps and qualitative chemical analyses. Results show that these embryo-like aggregates exhibit self-organization at the atomic level. Metallic elements content rises and consistent elemental polarization pattern of P and S in both mouse and human pluripotent stem cells were observed, indicating that neural differentiation and elemental polarization are strongly correlated.
authors.affiliation: Instituto de Física, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Simone C
authors.initials: SC
authors.lastname: Cardoso
authors.forename: Mariana P
authors.initials: MP
authors.lastname: Stelling
authors.forename: Bruna S
authors.initials: BS
authors.lastname: Paulsen
authors.forename: Stevens K
authors.initials: SK
authors.lastname: Rehen
mesh_terms: D000818:Animals; D016923:Cell Death / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004602:Elements; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D055495:Neurogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D011827:Radiation; D013052:Spectrometry, X-Ray Emission; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D013194:Staining and Labeling; D017356:Synchrotrons; D014404:Tubulin / Q000378:metabolism
chemical_list: D004602:Elements; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014404:Tubulin