title: Complete genome sequence of "Enterobacter lignolyticus" SCF1.
abstract: In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated "Enterobacter lignolyticus" SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of "E. lignolyticus" SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Deangelis
authors.forename: Patrik
authors.initials: P
authors.lastname: D'Haeseleer
authors.forename: Dylan
authors.initials: D
authors.lastname: Chivian
authors.forename: Julian L
authors.initials: JL
authors.lastname: Fortney
authors.forename: Jane
authors.initials: J
authors.lastname: Khudyakov
authors.forename: Blake
authors.initials: B
authors.lastname: Simmons
authors.forename: Hannah
authors.initials: H
authors.lastname: Woo
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.forename: Karen Walston
authors.initials: KW
authors.lastname: Davenport
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.forename: Amy
authors.initials: A
authors.lastname: Chen
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.forename: Nikos C
authors.initials: NC
authors.lastname: Kyrpides
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.forename: Terry C
authors.initials: TC
authors.lastname: Hazen