title: Random insertional mutagenesis in fungal genomes to identify virulence factors.
abstract: Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands. partha.santhanam@wur.nl
authors.forename: Parthasarathy
authors.initials: P
authors.lastname: Santhanam
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D018551:Solanum lycopersicum / Q000382:microbiology*; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology; D014170:Transformation, Genetic; D020172:Verticillium / Q000235:genetics* / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D037521:Virulence Factors