title: Evaluation of genotoxicity of Pseudevernia  furfuracea (L.) Zopf by RAPD analysis.
abstract: We investigated the suitability and applicability of Pseudevernia  furfuracea (L.) Zopf for environmental genotoxicity assessment. P.  furfuracea lichen specimens were collected from 10 different Pinus species,  in every 5 km, starting from around an iron-steel factory located in the  central area of Karabük Province up to Yenice Forest. The impact of the  pollution sources such as iron-steel factory, roads and railroads, industry,  heavy traffic, and waste treatment plants on the heavy metal accumulation in lichens  is known. DNA changes in P. furfuracea samples exposed naturally to  various polluted sites were analyzed by RAPD to know the influence of the  environmental pollution on the hereditary material of the organisms.  Twenty-five different primers were tested and 10 yielded clear and reproducible  bands. The present study shows the suitability of the lichen samples for the  detection of genotoxicity and also provides information about the level of  potential genotoxic agents around a steel mill.
authors.affiliation: Biotechnology Section, Department of Biology, Faculty of Science, University of Ankara, Tandogan, Ankara, Turkey. aras@science.ankara.edu.tr
authors.forename: S
authors.initials: S
authors.lastname: Aras
authors.forename: T
authors.initials: T
authors.lastname: Beyaztaş
authors.forename: D
authors.initials: D
authors.lastname: Cansaran-Duman
authors.forename: E
authors.initials: E
authors.lastname: Gökçe-Gündüzer
mesh_terms: D016678:Genome / Q000235:genetics; D005843:Geography; D008011:Lichens / Q000187:drug effects* / Q000235:genetics*; D009153:Mutagens / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods*; D014421:Turkey
chemical_list: D009153:Mutagens