title: Continuous aqueous two-phase extraction of human antibodies using a packed column.
abstract: The performance of a pilot scale packed differential contactor was evaluated for the continuous counter-current aqueous two-phase extraction (ATPE) of human immunoglobulin G (IgG) from a Chinese hamster ovary (CHO) cells supernatant (CS) enriched with pure protein. Preliminary studies have been firstly performed in order to select the dispersed phase (phosphate-rich or polyethylene glycol 3350 Da (PEG)-rich phase) and the column packing material. The PEG-rich phase has been selected as the dispersed phase and the stainless steel as the preferred material for the column packing bed since it was not wetted preferentially by the selected dispersed phase. Hydrodynamic studies have been also performed, and the experimental results were successfully adjusted to the Richardson-Zaki and Mísek equations, typically used for the conventional organic-aqueous two-phase systems. An experimental set-up combining the packed column with a pump mixer-settler stage showed to have the best performance and to be advantageous when compared to the IgG batch extraction. An IgG recovery yield of 85% could be obtained with about 50% of total contaminants and more than 85% of contaminant proteins removal. Mass transfer studies have revealed that the mass transfer was controlled by the PEG-rich phase. A higher efficiency could be obtained when using an extra pump mixer-settler stage and higher flow rates.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Department of Bioengineering, Technical University of Lisbon, Lisbon, Portugal.
authors.forename: P A J
authors.initials: PA
authors.lastname: Rosa
authors.forename: A M
authors.initials: AM
authors.lastname: Azevedo
authors.forename: S
authors.initials: S
authors.lastname: Sommerfeld
authors.forename: W
authors.initials: W
authors.lastname: Bäcker
authors.forename: M R
authors.initials: MR
authors.lastname: Aires-Barros
mesh_terms: D000818:Animals; D016466:CHO Cells; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design; D006801:Humans; D057446:Hydrodynamics; D007074:Immunoglobulin G / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation / Q000379:methods*; D008962:Models, Theoretical; D011092:Polyethylene Glycols; D012965:Sodium Chloride
chemical_list: D007074:Immunoglobulin G; D011092:Polyethylene Glycols; D012965:Sodium Chloride