title: Reverse protection assay: a tool to analyze transcriptional rates from individual promoters.
abstract: Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
authors.affiliation: Institut für Biologie (Genetik), Humboldt-Universität zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany. karsten.liere@rz.hu-berlin.de.
authors.forename: Yan O
authors.initials: YO
authors.lastname: Zubo
authors.forename: Victor V
authors.initials: VV
authors.lastname: Kusnetsov
authors.forename: Thomas
authors.initials: T
authors.lastname: Börner
authors.forename: Karsten
authors.initials: K
authors.lastname: Liere