title: Simultaneous transfer of cholesterol, triglycerides, and phospholipids to high-density lipoprotein in aging subjects with or without coronary artery disease.
abstract: OBJECTIVE
  To verify whether the capacity of high-density lipoprotein (HDL) to simultaneously receive nonesterified cholesterol, triglycerides, cholesteryl esters, and phospholipids changes with aging and the presence of coronary artery disease.
  
  
  METHODS
  Cross-sectional study with biochemical analyses.
  
  
  METHODS
  Eleven elderly patients with coronary artery disease (74 ± 5 years) were compared with the following groups of non-coronary artery disease subjects (referred to as "healthy"): 25 young (25 ± 5 years), 25 middle-aged (42 ± 6 years), and 25 elderly subjects (75 ± 8 years).
  
  
  METHODS
  Plasma samples were incubated with a nanoemulsion labeled with radioactive lipids; the transfer of the lipids from the nanoemulsion to the HDL was measured in chemically precipitated HDL. HDL size and paraoxonase-1 activity were also determined.
  
  
  RESULTS
  The transfer of cholesteryl esters and phospholipids to high-density lipoprotein was significantly greater (p<0.001) in healthy elderly subjects than in the middle-aged and younger subjects. Non-esterified cholesterol and triglyceride transfer was not different among these three groups. The HDL size was significantly greater (p<0.001) in healthy elderly subjects than in the middle-aged and younger subjects. The paraoxonase-1 activity was similar among the groups. Compared with healthy elderly subjects, coronary artery disease elderly subjects had significantly less (p<0.05) transfer of non-esterified cholesterol, triglycerides, and cholesteryl esters to the HDL and a significantly smaller (p<0.05) HDL size.
  
  
  CONCLUSIONS
  Because lipid transfer is enhanced in healthy elderly subjects but not in those with coronary artery disease, increasing lipid transfer to HDL may be a protective mechanism against the disease.
authors.affiliation: Lipid Metabolism Laboratory, Heart Institute, Faculdade de Medicina da Universidade de São Paulo, Brazil.
authors.forename: Carolina H M
authors.initials: CH
authors.lastname: Azevedo
authors.forename: Maurício
authors.initials: M
authors.lastname: Wajngarten
authors.forename: Ana C Lo
authors.initials: AC
authors.lastname: Prete
authors.forename: Jayme
authors.initials: J
authors.lastname: Diament
authors.forename: Raul C
authors.initials: RC
authors.lastname: Maranhão
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D043303:Aryldialkylphosphatase / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D003324:Coronary Artery Disease / Q000097:blood*; D004655:Emulsions; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D053758:Nanoparticles; D010316:Particle Size; D010743:Phospholipids / Q000097:blood*; D014280:Triglycerides / Q000097:blood*
chemical_list: D002788:Cholesterol Esters; D008076:Cholesterol, HDL; D004655:Emulsions; D010743:Phospholipids; D014280:Triglycerides; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human