title: Mucoadhesion evaluation of polysaccharide gels for vaginal application by using rheological and indentation measurements.
abstract: The influence of hyaluronic acid (HA) and fructooligosaccharides (FOS) addition on low methyl pectin (LMP) gelation has been investigated in order to produce adhesive gel-based microparticles suitable for the development of a vaginal delivery system of pro- and prebiotics. First, dynamic rheological measurements were performed on LMP/Ca(2+) gels with or without FOS and HA in presence or not of porcine stomach mucins. This rheological method is known to translate the interactions between polymer and mucins and then simulate the polymer bioadhesion potential. Nevertheless, as this method is disputed, in vitro and ex vivo indentation test measurements were also achieved in order to correlate the results obtained. Despite some different results, the overall tendency indicates that addition of HA and FOS enhanced the mucoadhesive properties of LMP gels. Moreover, gel-based microparticles obtained according to an emulsification/gelation method and composed by LMP 3% (w/v), FOS 5% (w/v) and HA 0.5% (w/v) displayed a mucoadhesive potential adapted to vaginal delivery system.
authors.affiliation: Université de Lyon, Université Claude Bernard Lyon 1, UMR 5180 Laboratoires des sciences analytiques, Villeurbanne, France.
authors.forename: D
authors.initials: D
authors.lastname: Pliszczak
authors.forename: C
authors.initials: C
authors.lastname: Bordes
authors.forename: S
authors.initials: S
authors.lastname: Bourgeois
authors.forename: P
authors.initials: P
authors.lastname: Marote
authors.forename: H
authors.initials: H
authors.lastname: Zahouani
authors.forename: S
authors.initials: S
authors.lastname: Tupin
authors.forename: C Pailler
authors.initials: CP
authors.lastname: Mattei
authors.forename: P
authors.initials: P
authors.lastname: Lantéri
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D000282:Administration, Intravaginal; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D055119:Elastic Modulus / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005240:Feasibility Studies; D005260:Female; D005782:Gels / Q000494:pharmacology*; D006041:Goats; D006820:Hyaluronic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008422:Materials Testing; D009077:Mucins / Q000494:pharmacology*; D009844:Oligosaccharides / Q000494:pharmacology; D010368:Pectins / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D012212:Rheology / Q000379:methods*; D034421:Sus scrofa
chemical_list: D005782:Gels; D009077:Mucins; D009844:Oligosaccharides; D011134:Polysaccharides; C116580:fructooligosaccharide; D010368:Pectins; D006820:Hyaluronic Acid