title: The interaction of canine plasminogen with Streptococcus pyogenes enolase: they bind to one another but what is the nature of the structures involved?
abstract: For years it has been clear that plasminogen from different sources and enolase from different sources interact strongly. What is less clear is the nature of the structures required for them to interact. This work examines the interaction between canine plasminogen (dPgn) and Streptococcus pyogenes enolase (Str enolase) using analytical ultracentrifugation (AUC), surface plasmon resonance (SPR), fluorescence polarization, dynamic light scattering (DLS), isothermal titration calorimetry (ITC), and simple pull-down reactions. Overall, our data indicate that a non-native structure of the octameric Str enolase (monomers or multimers) is an important determinant of its surface-mediated interaction with host plasminogen. Interestingly, a non-native structure of plasminogen is capable of interacting with native enolase. As far as we can tell, the native structures resist forming stable mixed complexes.
authors.affiliation: Department of Biology, Concordia University, Montreal, Quebec, Canada. krnbltt@alcor.concordia.ca
authors.forename: M Judith
authors.initials: MJ
authors.lastname: Kornblatt
authors.forename: Jack A
authors.initials: JA
authors.lastname: Kornblatt
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hancock
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002021:Buffers; D002151:Calorimetry; D011232:Chemical Precipitation / Q000187:drug effects; D004285:Dogs; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D008823:Micelles; D008958:Models, Molecular; D010743:Phospholipids / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000378:metabolism*; D010958:Plasminogen / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D019163:Reducing Agents / Q000494:pharmacology; D012996:Solutions; D013297:Streptococcus pyogenes / Q000187:drug effects / Q000201:enzymology*; D020349:Surface Plasmon Resonance; D013499:Surface Properties / Q000187:drug effects; D014461:Ultracentrifugation
chemical_list: D002021:Buffers; D008823:Micelles; D010743:Phospholipids; D019163:Reducing Agents; D012996:Solutions; D010958:Plasminogen; D010751:Phosphopyruvate Hydratase