title: Subdiffraction scattered light imaging of gold nanoparticles using structured illumination.
abstract: A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100 nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428 nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.
authors.affiliation: National Synchrotron Radiation Research Center, Hsinchu Science Park, Hsinchu, Taiwan.
authors.forename: Bo-Jui
authors.initials: BJ
authors.lastname: Chang
authors.forename: Shiuan Huei
authors.initials: SH
authors.lastname: Lin
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Chou
authors.forename: Su-Yu
authors.initials: SY
authors.lastname: Chiang
mesh_terms: D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D007834:Lasers; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy / Q000379:methods; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry; D055095:Optics and Photonics; D012542:Scattering, Radiation
chemical_list: D006046:Gold