title: Identification of HR-inducing cDNAs from plant pathogens via a Gateway(®)-compatible binary Potato virus X-expression vector.
abstract: Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands. Peter.vanesse@wur.nl
authors.forename: H Peter
authors.initials: HP
authors.lastname: van Esse
mesh_terms: D016960:Agrobacterium tumefaciens / Q000502:physiology / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D060467:Disease Resistance; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics*; D057166:High-Throughput Screening Assays / Q000379:methods; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D010944:Plants / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D017863:Potexvirus / Q000235:genetics*
chemical_list: D018076:DNA, Complementary