title: Partial blocking of mouse DSPP processing by substitution of Gly(451)-Asp(452) bond suggests the presence of secondary cleavage site(s).
abstract: Dentin sialophosphoprotein (DSPP) in the extracellular matrix of dentin is cleaved into dentin sialoprotein and dentin phosphoprotein, which originate from the NH(2)-terminal and COOH-terminal regions of DSPP, respectively. In the proteolytic processing of mouse DSPP, the peptide bond at Gly(451)-Asp(452) has been shown to be cleaved by bone morphogenetic protein 1 (BMP1)/Tolloid-like metalloproteinases. In this study, we generated transgenic mice expressing a mutant DSPP in which Asp(452) was substituted by Ala(452). Protein chemistry analyses of extracts from the long bone of these transgenic mice showed that the D452A substitution partially blocked DSPP processing in vivo. When the full-length form of mutant DSPP (designated "D452A-DSPP") isolated from the transgenic mice was treated with BMP1 in vitro, a portion of the D452A-DSPP was cleaved, suggesting the presence of secondary peptide bond(s) that can be broken by BMP1. To identify the potential secondary DSPP cleavage site(s), site-directed mutagenesis was performed to generate nine DNA constructs expressing DSPP-bearing substitutions at potential scission sites. These different types of mutant DSPP made in eukaryotic cell lines were treated with BMP1 and the digestion products were assessed by Western immunoblotting. All of the mutant DSPP molecular species were partially cleaved by BMP1, giving rise to a protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis similar to that of normal dentin sialoprotein. Taken together, we concluded that in addition to the peptide bond Gly(451)-Asp(452), there must be a cryptic cleavage site or sites close to Asp(452) in the mouse DSPP that can be cleaved by BMP1.
authors.affiliation: Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, TX 75246, USA.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Zhu
authors.forename: Monica
authors.initials: M
authors.lastname: Prasad
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Lu
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Albert
authors.initials: A
authors.lastname: Yamoah
authors.forename: Jian Q
authors.initials: JQ
authors.lastname: Feng
authors.forename: Chunlin
authors.initials: C
authors.lastname: Qin
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D055395:Bone Morphogenetic Protein 1 / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D050505:Mutant Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012795:Sialoglycoproteins / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics; D000099311:Dentin Sialophosphoprotein
chemical_list: D055395:Bone Morphogenetic Protein 1; D016326:Extracellular Matrix Proteins; D050505:Mutant Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein