title: Coatings from micropatterned sulfobetaine polymer brushes as substrates for MC3T3-E1 cells.
abstract: In the last decades, polymer brush coatings have proven to be excellent anti-fouling materials by preventing protein adhesion. When using this property to restrict cell growth laterally in cell culture, it is crucial to ensure that other cell functions remain unaffected. The present study therefore examines MC3T3-E1 cell growth and morphology on patterned PSBMA brush substrates and probes their proliferation potential at mRNA level. The osteoblastic cells display a more elongated morphology than cells on the control substrates, but show no sign of elevated levels of the apoptosis marker p53 or diminished levels of Ki-67 or H4, which serve as indicators of proliferation. Therefore, patterned polymer brushes do not seem to influence cells in their proliferation state and are suitable cell culture substrates. Nevertheless, the use of polymer brush surfaces in long-term cell culture was found to be limited by their instability in cell culture medium.
authors.affiliation: Chair of Solid State Chemistry and Material Science, Institute of Physics, University of Augsburg, Augsburg, Germany.
authors.forename: Annina
authors.initials: A
authors.lastname: Steinbach
authors.forename: Andrea
authors.initials: A
authors.lastname: Tautzenberger
authors.forename: Anita
authors.initials: A
authors.lastname: Ignatius
authors.forename: Manuela
authors.initials: M
authors.lastname: Pluntke
authors.forename: Othmar
authors.initials: O
authors.lastname: Marti
authors.forename: Dirk
authors.initials: D
authors.lastname: Volkmer
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017209:Apoptosis; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D019394:Ki-67 Antigen / Q000096:biosynthesis; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010006:Osteoblasts / Q000166:cytology; D011108:Polymers / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D011108:Polymers; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D001622:Betaine; C483727:sulfobetaine