title: Gender difference in proteome of brown adipose tissues between male and female rats exposed to a high fat diet.
abstract: BACKGROUND
  Although the importance of gender as a key determinant in health and illness has been recognized for a long time, systematic studies of gender differences in medicine are still lacking. We hypothesized that interscapular brown adipocyte tissue (BAT), is not only a key tissue contributing to energy expenditure, but also regulates diet-induced thermogenesis, and may be an ideal target for studying gender differences in obesity development in response to a high fat diet (HFD).
  
  
  METHODS
  We therefore performed differential proteome analysis of BAT from lean and obese rats of both genders fed a HFD using 2-DE combined with MALDI-TOF-MS.
  
  
  RESULTS
  When exposed to a HFD, male rats gained more body weight with increased values of plasma biochemical parameters than did female rats. Among 595 matched spots, 48 differentially expressed identified spots showed significant gender differences, whereas 7 proteins showed no gender differences, but did show a HFD response.
  
  
  CONCLUSIONS
  Proteomic investigations into gender-dimorphic protein modulation in BAT may provide conclusive results showing higher expression of numerous proteins involved in thermogenesis and fat oxidation as well as lower expression of proteins contributing to fat synthesis in female rats than in male rats.
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea.
authors.forename: Duk Kwon
authors.initials: DK
authors.lastname: Choi
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Oh
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Rajib
authors.initials: R
authors.lastname: Mukherjee
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D020543:Proteome; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome