title: Clinicopathological correlates and prognostic significance of glutathione S-transferase Pi expression in 468 patients after potentially curative resection of node-positive colonic cancer.
abstract: OBJECTIVE
  This study investigated the association between glutathione S-transferase Pi (GST Pi) expression, histopathology and overall survival in 468 patients after resection of stage C colonic adenocarcinoma.
  
  
  RESULTS
  Data were drawn from a prospective hospital registry of consecutive bowel cancer resections with a minimum follow-up of 5 years. Nuclear and cytoplasmic GST Pi expression, assessed by both intensity of staining and percentage of stained cells at both the central part of the tumour and the invasive tumour front, were evaluated retrospectively by tissue microarray immunohistochemistry on archival specimens. The most effective measure of GST Pi expression was the percentage of immunostained nuclei in central tumour tissue, where >40% stained was associated significantly with high grade, invasion beyond the muscularis propria, involvement of a free serosal surface or apical node, and invasion into an adjacent organ or structure. After adjustment of other predictors, GST Pi expression remained independently prognostic for reduced overall survival (hazard ratio 1.4, P = 0.002).
  
  
  CONCLUSIONS
  In patients with clinicopathological stage C colonic cancer, GST Pi expression is associated with features of tumour aggressiveness and with reduced overall survival. Further appropriately designed studies should aim to discover whether GST Pi can predict response to adjuvant chemotherapy.
authors.affiliation: Department of Anatomical Pathology, Concord Hospital, The University of Sydney, Sydney, New South Wales, Australia.
authors.forename: King L
authors.initials: KL
authors.lastname: Tan
authors.forename: Lucy
authors.initials: L
authors.lastname: Jankova
authors.forename: Charles
authors.initials: C
authors.lastname: Chan
authors.forename: Caroline L-S
authors.initials: CL
authors.lastname: Fung
authors.forename: Candice
authors.initials: C
authors.lastname: Clarke
authors.forename: Betty P C
authors.initials: BP
authors.lastname: Lin
authors.forename: Graham
authors.initials: G
authors.lastname: Robertson
authors.forename: Mark
authors.initials: M
authors.lastname: Molloy
authors.forename: Pierre H
authors.initials: PH
authors.lastname: Chapuis
authors.forename: Les
authors.initials: L
authors.lastname: Bokey
authors.forename: Owen F
authors.initials: OF
authors.lastname: Dent
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Clarke
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology* / Q000601:surgery; D005260:Female; D051549:Glutathione S-Transferase pi / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D051549:Glutathione S-Transferase pi