title: Approaches for gene targeting and targeted gene expression in plants.
abstract: Transgenic science and technology are fundamental to state-of-the-art plant molecular genetics and crop improvement. The new generation of technology endeavors to introduce genes 'stably' into 'site-specific' locations and in 'single copy' without the integration of extraneous vector 'backbone' sequences or selectable markers and with a 'predictable and consistent' expression. Several similar strategies and technologies, which can push the development of 'smart' genetically modified plants with desirable attributes, as well as enhance their consumer acceptability, are discussed in this review.
authors.affiliation: Division of Plant Breeding and Genetics; Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir; Shalimar, India. amjadhusaini@yahoo.com
authors.forename: Amjad Masood
authors.initials: AM
authors.lastname: Husaini
authors.forename: Zerka
authors.initials: Z
authors.lastname: Rashid
authors.forename: Reyaz-ul Rouf
authors.initials: RU
authors.lastname: Mir
authors.forename: Bushra
authors.initials: B
authors.lastname: Aquil
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D018390:Gene Targeting / Q000379:methods* / Q000639:trends; D017343:Genes, Plant / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods / Q000639:trends; D063245:Plant Development; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D019076:Transgenes / Q000235:genetics