title: 3,3'-diethylthiatricarbocyanine iodide: a highly sensitive chiroptical reporter of DNA helicity and sequence.
abstract: Using UV-vis absorption and circular dichroism (CD) spectroscopies, we explored the binding interactions of 3,3'-diethylthiatricarbocyanine iodide (Cy7) with polynucleotides of different sequences and helicity. CD showed to be a very diagnostic tool giving different spectroscopic chiroptical signatures for all explored DNA sequences upon Cy7 binding. Cy7 was able to spectroscopically discriminate between the right handed B-DNA of poly(dG-dC)(2) and its left handed Z-DNA counterpart induced by spermine or Co(III)hexamine via nearly opposite induced circular dichroic signal.
authors.affiliation: Department of Chemistry, University of Wyoming, Laramie, WY 82071, USA; E-Mails:  jchoi4@uwyo.edu  (J.K.C.);  adurso@unict.it  (A.D.);  mshabbir@uwyo.edu  (M.S.-H.).
authors.forename: Jung Kyu
authors.initials: JK
authors.lastname: Choi
authors.forename: Alessandro
authors.initials: A
authors.lastname: D'Urso
authors.forename: Mitch
authors.initials: M
authors.lastname: Trauernicht
authors.forename: Murtaza
authors.initials: M
authors.lastname: Shabbir-Hussain
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Holmes
authors.forename: Milan
authors.initials: M
authors.lastname: Balaz
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry*; D002942:Circular Dichroism; D059371:DNA, B-Form / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000737:chemistry; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D059371:DNA, B-Form; D043542:DNA, Z-Form; D011089:Polydeoxyribonucleotides; C501744:indotricarbocyanine; C025905:3,3'-diethylthiatricarbocyanine; C019530:poly(dC-dG)