title: Relationship between the expression level of SLA-DQA and Escherichia coli F18 infection in piglets.
abstract: The expression of SLA-DQA was assayed by Real-time PCR to analyze the differential expression between ETEC F18-resistant and -sensitive post-weaning piglets, and then to compare the expression levels of SLA-DQA in 11 different tissues from 8-, 18-, 30- and 35-day-old ETEC F18-resistant piglets, which aimed at discussing the role of SLA-DQA in resistance to ETEC F18. The results showed that SLA-DQA is broadly expressed in 11 tissues with the highest expression level in lymph nodes, and a relatively higher expression level in lung, spleen, jejunum, and duodenum. In tissues of lymph node, lung, spleen, jejunum, and duodenum, the mRNA expression of SLA-DQA in resistant individuals was significantly higher than that in sensitive ones (P<0.05). In most tissues, the expression of SLA-DQA increased from 8 to 18 and 30 days (weaning day), and increased persistently to 35 days of post-weaning. Expression levels of SLA-DQA on 35 days in most tissues were significant higher than that on 8, 18 and 30 days (P<0.05). The results demonstrated that the resistance to ETEC F18 in post-weaning piglets is related to up-regulation of mRNA expression of SLA-DQA to a certain extent. The analysis suggested that SLA-DQA may be not the direct immune factor that resisted the Escherichia coli F18, but perhaps enhanced humoral immunity and cell immunity to reduce the transmembrane signal transduction of ETEC F18 bacterial LPS and then led to the resistance to ETEC F18 in piglets.
authors.affiliation: College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, China. wenbinbao74@yahoo.com
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Bao
authors.forename: Lan
authors.initials: L
authors.lastname: Ye
authors.forename: Chen
authors.initials: C
authors.lastname: Zi
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Zhang-yuan
authors.initials: ZY
authors.lastname: Pan
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Zhu
authors.forename: Xue-gen
authors.initials: XG
authors.lastname: Huang
authors.forename: Sheng-long
authors.initials: SL
authors.lastname: Wu
mesh_terms: D000818:Animals; D060467:Disease Resistance / Q000235:genetics*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics; D004927:Escherichia coli Infections / Q000235:genetics* / Q000276:immunology; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000276:immunology; D013997:Time Factors
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen