title: Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes.
abstract: Erythroid biology research involving rhesus macaques has been applied to several topics including malaria, hemoglobinopathy and gene therapy research. However, analyses of the rhesus red blood cells are limited by the inability to identify and sort those cells in research blood samples using flow cytometry. Here it is reported that the BRIC 6 hybridoma clone raised to the human erythroid surface molecule (referred to as CD233, Band 3, AE1, or SLC4A1) produces cross-reactive and erythroid-specific antibodies for flow cytometric detection and sorting of rhesus macaque erythrocytes.
authors.affiliation: Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Colleen
authors.initials: C
authors.lastname: Byrnes
authors.forename: Y
authors.initials: Y
authors.lastname: Terry Lee
authors.forename: Robert E
authors.initials: RE
authors.lastname: Donahue
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Miller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology*; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D008253:Macaca mulatta / Q000276:immunology*; D008969:Molecular Sequence Data
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D000911:Antibodies, Monoclonal; D006021:Glycophorins; C454201:SLC4A1 protein, human