title: Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.
abstract: Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful method for the specific identification of harmful Vibrio strains is needed. We developed a PCR-Ligase Detection Reaction-Universal Array (PCR-LDR-UA) assay for the simultaneous identification of pathogenic vibrios and detection of virulence coding genes. The entire procedure was validated on a total of 31 reference strains and isolates from clinical and environmental samples, as well as on bivalve tissue homogenates infected with different strains of target Vibrio species. Twenty-three shellfish samples directed to human consumption were successfully screened, thus demonstrating that the developed microarray-based platform could be a reliable and sensitive detection tool for the identification of harmful Vibrio strains in seafood.
authors.affiliation: Department of Experimental Evolutionary Biology, University of Bologna, Italy. alessia.cariani@unibo.it
authors.forename: Alessia
authors.initials: A
authors.lastname: Cariani
authors.forename: Annamaria
authors.initials: A
authors.lastname: Piano
authors.forename: Clarissa
authors.initials: C
authors.lastname: Consolandi
authors.forename: Marco
authors.initials: M
authors.lastname: Severgnini
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
authors.forename: Giada
authors.initials: G
authors.lastname: Caredda
authors.forename: Marco
authors.initials: M
authors.lastname: Candela
authors.forename: Patrizia
authors.initials: P
authors.lastname: Serratore
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
authors.forename: Fausto
authors.initials: F
authors.lastname: Tinti
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000235:genetics; D006801:Humans; D008025:Ligases / Q000032:analysis; D046228:Microarray Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012016:Reference Values; D017747:Seafood / Q000382:microbiology*; D012758:Shellfish / Q000382:microbiology*; D014733:Vibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014735:Vibrio Infections; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044164:Vibrio mimicus / Q000235:genetics; D014736:Vibrio parahaemolyticus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D008025:Ligases