title: Development and in vivo imaging of a PET/MRI nanoprobe with enhanced NIR fluorescence by dye encapsulation.
abstract: OBJECTIVE
  To monitor cells in vivo or to detect the sentinel lymph node, we developed a PET/MRI silica nanoprobe with an enhanced near-infrared fluorescence signal.
  
  
  METHODS
  We developed enhanced near-infrared fluorescent (NIRF) magnetic silica nanoparticles, MNP-SiO2(NIR797), that encapsulate NIRF dye in the silica. We applied this probe to visualizing cells in the deep tissue of mice using NIRF imaging. After labeling with a radioisotope, (68)Ga, on the surface of MNP-SiO2(NIR797), we injected it into the forepaw of mice to visualize the sentinel lymph node.
  
  
  RESULTS
  This encapsulated nanoprobe showed enhancement of fluorescent intensity and stability compared with the nanoprobe, which had the same dyes on the surface of the silica nanoparticles. We also obtained multimodal in vivo imaging of (68)Ga-{MNP-SiO2(NIR797)} applied to sentinel lymph node detection of mice using PET/MRI/NIRF images.
  
  
  CONCLUSIONS
  This multimodal nanoprobe with enhanced fluorescence may provide a useful tool for imaging diagnostics and cell tracking.
authors.affiliation: R&D Center, Biterials Co., Ltd., Seoul.
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Kim
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.forename: Eunju Lee
authors.initials: EL
authors.lastname: Tae
authors.forename: Hyewon
authors.initials: H
authors.lastname: Youn
authors.forename: Daehong
authors.initials: D
authors.lastname: Kim
authors.forename: Seok-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Jung-Taek
authors.initials: JT
authors.lastname: Kwon
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.forename: Yun-Sang
authors.initials: YS
authors.lastname: Lee
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Jeong
authors.forename: June-Key
authors.initials: JK
authors.lastname: Chung
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D058948:Cell Tracking / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D015336:Molecular Probe Techniques; D053769:Nanocapsules / Q000737:chemistry*; D049268:Positron-Emission Tomography / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004396:Coloring Agents; D003287:Contrast Media; D053769:Nanocapsules