title: Identification of cis-acting promoter elements in cold- and dehydration-induced transcriptional pathways in Arabidopsis, rice, and soybean.
abstract: The genomes of three plants, Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and soybean (Glycine max), have been sequenced, and their many genes and promoters have been predicted. In Arabidopsis, cis-acting promoter elements involved in cold- and dehydration-responsive gene expression have been extensively analysed; however, the characteristics of such cis-acting promoter sequences in cold- and dehydration-inducible genes of rice and soybean remain to be clarified. In this study, we performed microarray analyses using the three species, and compared characteristics of identified cold- and dehydration-inducible genes. Transcription profiles of the cold- and dehydration-responsive genes were similar among these three species, showing representative upregulated (dehydrin/LEA) and downregulated (photosynthesis-related) genes. All (4(6) = 4096) hexamer sequences in the promoters of the three species were investigated, revealing the frequency of conserved sequences in cold- and dehydration-inducible promoters. A core sequence of the abscisic acid-responsive element (ABRE) was the most conserved in dehydration-inducible promoters of all three species, suggesting that transcriptional regulation for dehydration-inducible genes is similar among these three species, with the ABRE-dependent transcriptional pathway. In contrast, for cold-inducible promoters, the conserved hexamer sequences were diversified among these three species, suggesting the existence of diverse transcriptional regulatory pathways for cold-inducible genes among the species.
authors.affiliation: Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
authors.forename: Kyonoshin
authors.initials: K
authors.lastname: Maruyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Todaka
authors.forename: Junya
authors.initials: J
authors.lastname: Mizoi
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kidokoro
authors.forename: Satoko
authors.initials: S
authors.lastname: Matsukura
authors.forename: Hironori
authors.initials: H
authors.lastname: Takasaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Yoshiharu Y
authors.initials: YY
authors.lastname: Yamamoto
authors.forename: Kyouko
authors.initials: K
authors.lastname: Yoshiwara
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
mesh_terms: D000040:Abscisic Acid / Q000032:analysis; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D016000:Cluster Analysis; D003080:Cold Temperature; D017124:Conserved Sequence; D003681:Dehydration; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D059372:Nucleotide Motifs; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C114578:DREB1A protein, Arabidopsis; C114579:DREB2A protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid