title: Prospective study to validate HpOne in the diagnosis of Helicobacter pylori infection.
abstract: BACKGROUND
  The HpOne test is a new five-minute rapid urease test developed for the rapid detection of Helicobacter pylori infection during gastroscopy. However, evidence in the literature supporting its use clinically is scarce. The most commonly used rapid urease test remains the Campylobacter-like organism (CLO) test, which generates accurate readings only after 24 hours. The aim of this study was to evaluate the efficacy of the HpOne test in our local population.
  
  
  METHODS
  From August 2007 to May 2008, consecutive patients undergoing gastroscopy for various indications were recruited into this prospective study. Patients who were pregnant, lactating, on proton pump inhibitors, antibiotics, immunosuppressants or had previous gastric surgery were excluded. During gastroscopy, six gastric mucosal biopsies were taken; three from the body and three from the antrum. One body and one antral biopsy were used for each of the HpOne test, CLO test and histology. Results of the HpOne and CLO tests were then compared against the gold standard of histology.
  
  
  RESULTS
  Of the 149 patients recruited, 82 (55 percent) were men and 67 (45 percent) were women. The prevalence of Helicobacter pylori infection was 38.9 percent (n is 58). The sensitivity and specificity of the HpOne test were 65.5 percent and 85.7 percent, respectively, while those for the CLO test were 63.8 percent and 84.6 percent, respectively.
  
  
  CONCLUSIONS
  The HpOne test is as efficacious as the CLO test, with the added advantage of yielding results faster. It is thus a superior alternative and should be considered for clinical use.
authors.affiliation: Yong Loo Lin School of Medicine, 1E Kent Ridge Road, NUHS Tower Block Level 11, Singapore 119228.
authors.forename: S W Y
authors.initials: SW
authors.lastname: Chan
authors.forename: K Y
authors.initials: KY
authors.lastname: Tan
authors.forename: A K S
authors.initials: AK
authors.lastname: Cheng
authors.forename: K S W
authors.initials: KS
authors.lastname: Mak
authors.forename: A T
authors.initials: AT
authors.lastname: Aye
authors.forename: M
authors.initials: M
authors.lastname: Pang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002167:Campylobacter / Q000378:metabolism; D005260:Female; D005773:Gastroscopy / Q000009:adverse effects; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014510:Urease / Q000032:analysis / Q000378:metabolism
chemical_list: D011933:Reagent Kits, Diagnostic; D014510:Urease