title: Molecular and antigenic characterization of H9N2 avian influenza virus isolates from chicken flocks between 1998 and 2007 in China.
abstract: Despite extensive vaccination, H9N2 subtype influenza A viruses (IAVs) have prevailed in chicken populations in China. H9N2 IAVs have been a major cause of respiratory disease and reduced egg production, resulting in great economic losses to the Chinese poultry industry. In attempt to find reasons for lack of adequate protection by commercial vaccines, 41 H9N2 viruses isolated from chicken flocks in various regions of China through surveillance between 1998 and 2007 were systemically analyzed using molecular and serological methods in comparison to IAV Ck/Shandong/6/96 and Ck/Shanghai/F/98 that have been used in a majority of commercial vaccines for H9N2 in China since 1998. The analyses showed that the field isolates were predominantly of Beijing/94 lineage and underwent rapid genetic and antigenic changes, forming several antigenic groups. Comparisons between the field isolates and vaccine strains revealed that a majority of the field isolates examined were antigenically distinct from the vaccine strains to some extent. Therefore, the rapid antigenic evolution of H9N2 IAV and resulting antigenic difference from the earlier vaccine strains appears to be a key factor for suboptimal control of H9N2 IAV in China, emphasizing that the vaccine strain should be updated in a timely manner through surveillance and accompanying laboratory evaluation of contemporary viruses for antigenic similarity with existing vaccine strains.
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Yin
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Bi
authors.forename: Shouchun
authors.initials: S
authors.lastname: Wang
authors.forename: Shouzhen
authors.initials: S
authors.lastname: Xu
authors.forename: Jianlin
authors.initials: J
authors.lastname: Wang
authors.forename: Shun
authors.initials: S
authors.lastname: Zhou
authors.forename: Tingting
authors.initials: T
authors.lastname: Sun
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Yoon
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D005075:Biological Evolution; D002645:Chickens / Q000821:virology*; D002681:China / Q000453:epidemiology; D056738:Cross Protection; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D053127:Influenza A Virus, H9N2 Subtype / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000008:administration & dosage; D005585:Influenza in Birds / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D009500:Neutralization Tests / Q000662:veterinary; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D020539:Sequence Analysis, Protein
chemical_list: D000956:Antigens, Viral; D007252:Influenza Vaccines; D012367:RNA, Viral