title: Nucleic acid probe containing fluorescent tricyclic base-linked acyclonucleoside for detection of single nucleotide polymorphisms.
abstract: The development of a reliable and simple method for detecting single nucleotide polymorphisms (SNPs), common genetic variations in the human genome, is currently an important research area because SNPs are important for identifying disease-causing genes and for pharmacogenetic studies. Here, we developed a novel method for SNP detection. We designed and synthesized DNA probes containing a fluorescent tricyclic base-linked acyclonucleoside P. The type of nucleobases involved in the SNP sites in the DNA and RNA targets could be determined using four DNA probes containing P. Thus, this system would provide a novel and simple method for detecting SNPs in DNA and RNA targets.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kinji
authors.initials: K
authors.lastname: Furukawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Hattori
authors.forename: Tokimitsu
authors.initials: T
authors.lastname: Ohki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015894:Genome, Human; D006801:Humans; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide; D011743:Pyrimidines / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D044366:Transition Temperature
chemical_list: C571597:8-amino-3-(2,3-dihydroxypropyl)imidazo(4',5'-5,6)pyrido(2,3-d)pyrimidine; D015342:DNA Probes; D005456:Fluorescent Dyes; D007093:Imidazoles; D009838:Oligodeoxyribonucleotides; D011743:Pyrimidines; D012313:RNA; D004247:DNA