title: Antitumor activity of Dioscorea bulbifera L. rhizome in vivo.
abstract: Antitumor activities of water extract (fraction A), ethanol extract (fraction B), ethyl acetate extract (fraction C), non-ethyl acetate extract (fraction D) and compound diosbulbin B isolated from Dioscorea bulbifera L. (DB) were investigated in vivo in this present study. The results showed that fractions B and C both decreased tumor weight in S180 and H22 tumor cells bearing mice, while fractions A and D had no such effect. Furthermore, fraction C altered the weight of spleen and thymus, and the amounts of total leukocytes, lymphocytes and neutrophils in tumor-bearing mice. Further results showed that compound diosbulbin B demonstrated anti-tumor effects in the dose-dependent manner at the dosage of 2 to 16 mg/kg without significant toxicity in vivo. Furthermore, on the basis of chemical analysis of the above extracts by high-performance liquid chromatography (HPLC) with a diode array detector (DAD), diosbulbin B was found to be the major antitumor bioactive component of DB. These results suggest that DB has potential anti-tumor effects which may be related to influencing the immune system for the first time, and the compound diosbulbin B is the major antitumor component of DB.
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Ji
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Branford-White
authors.forename: Zai-Yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Kai-Kai
authors.initials: KK
authors.lastname: Shen
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.forename: Zheng-Tao
authors.initials: ZT
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D027742:Dioscorea / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D012513:Sarcoma, Experimental / Q000178:diet therapy; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D006576:Heterocyclic Compounds, 4 or More Rings; C569454:diosbulbin B