title: Simultaneous determination of tetrandrine and fangchinoline in herbal medicine Stephania tetrandra S. Moore by liquid chromatography with electrochemical detection.
abstract: A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile-ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95-105%, while the precision expressed as repetition of peak area was lower than 2.7%.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan 430071, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
authors.forename: Shenglan
authors.initials: S
authors.lastname: Li
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D044182:Benzylisoquinolines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D028321:Plant Preparations / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D031609:Stephania tetrandra / Q000737:chemistry*
chemical_list: D044182:Benzylisoquinolines; D010936:Plant Extracts; D028321:Plant Preparations; C009438:tetrandrine; C060802:fangchinoline