title: Genotypic characterization of non-O157 Shiga toxin-producing Escherichia coli in beef abattoirs of Argentina.
abstract: The non-O157 Shiga toxin-producing Escherichia coli (STEC) contamination in carcasses and feces of 811 bovines in nine beef abattoirs from Argentina was analyzed during a period of 17 months. The feces of 181 (22.3%) bovines were positive for non-O157 STEC, while 73 (9.0%)  of the carcasses showed non-O157 STEC contamination. Non-O157 STEC strains isolated from feces (227) and carcasses (80) were characterized. The main serotypes identified were O178:H19, O8:H19, O130:H11, and O113:H21, all of which have produced sporadic cases of hemolytic-uremic syndrome in  Argentina and worldwide. Twenty-two (7.2%) strains carried a fully virulent stx/eae/ehxA genotype. Among them, strains of serotypes O103:[H2], O145:NM, and O111:NM represented 4.8% of the isolates. Xba I pulsed-field gel electrophoresis pattern analysis showed 234 different patterns,  with 76 strains grouped in 30 clusters. Nine of the clusters grouped strains isolated from feces and from carcasses of the same or different bovines in a lot, while three clusters were comprised of strains distributed in more than one abattoir. Patterns AREXSX01.0157, AREXBX01.0015, and AREXPX01.0013  were identified as 100% compatible with the patterns of one strain isolated from a hemolytic-uremic syndrome case and two strains previously isolated from beef medallions, included in the Argentine PulseNet Database. In this survey, 4.8% (39 of 811) of the bovine carcasses appeared to be contaminated  with nonO157 STEC strains potentially capable of producing sporadic human disease, and a lower proportion (0.25%) with strains able to produce outbreaks of severe disease.
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria-INTA, CC. 77, B1708WAB Morón, Provincia de Buenos Aires, Argentina. mmasana@cnia.inta.gov.ar
authors.forename: M O
authors.initials: MO
authors.lastname: Masana
authors.forename: B A
authors.initials: BA
authors.lastname: D'Astek
authors.forename: P M
authors.initials: PM
authors.lastname: Palladino
authors.forename: L
authors.initials: L
authors.lastname: Galli
authors.forename: L L
authors.initials: LL
authors.lastname: Del Castillo
authors.forename: C
authors.initials: C
authors.lastname: Carbonari
authors.forename: G A
authors.initials: GA
authors.lastname: Leotta
authors.forename: E
authors.initials: E
authors.lastname: Vilacoba
authors.forename: K
authors.initials: K
authors.lastname: Irino
authors.forename: M
authors.initials: M
authors.lastname: Rivas
mesh_terms: D000003:Abattoirs; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D002417:Cattle / Q000382:microbiology*; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks / Q000517:prevention & control; D005243:Feces / Q000382:microbiology; D005260:Female; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D005838:Genotype; D006463:Hemolytic-Uremic Syndrome / Q000453:epidemiology / Q000517:prevention & control; D006801:Humans; D008297:Male; D015995:Prevalence; D018570:Risk Assessment; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial