title: Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.
abstract: Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.
authors.affiliation: Department of Physics, McGill University, Montréal, Québec, Canada.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Sergeev
authors.forename: Jody L
authors.initials: JL
authors.lastname: Swift
authors.forename: Antoine G
authors.initials: AG
authors.lastname: Godin
authors.forename: Paul W
authors.initials: PW
authors.lastname: Wiseman
mesh_terms: D000818:Animals; D016466:CHO Cells; D016000:Cluster Analysis; D006224:Cricetinae; D003412:Cricetulus; D066246:ErbB Receptors / Q000737:chemistry* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence
chemical_list: D008024:Ligands; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D066246:ErbB Receptors