title: Transcriptome analysis of tree peony during chilling requirement fulfillment: assembling, annotation and markers discovering.
abstract: Tree peony (Paeonia suffruticosa Andrews) is a well-known horticultural and medicinal plant. The flower buds must go through a period of endo-dormancy before bud sprouting in winter, but very little information concerned with dormancy release is available. We obtained 625,342 sequencing reads with massive parallel pyrosequencing on the Roche 454 GS FLX platform (mean length: 358.1bp). De novo assemblies yielded 23,652 contigs and singletons. 15,284 contigs longer than 300bp were further annotated, among them 12,345 ESTs showed significant similarity with sequences present in public databases (with an E-value <1e-10). 484 putative transcription factors were obtained. In addition, 2253 potential Simple Sequence Repeats (SSR) loci were identified in the 454-ESTs. Total 149 pairs of primers were designed, and 121 pairs were amplified successfully in initial screening. In addition, 73 pairs of primers displayed polymorphism. This sequence collection provides a significant resource for gene discovery during endo-dormancy of tree peony.
authors.affiliation: Universities of Shandong Province, Qingdao, China. spgai@qau.edu.cn
authors.forename: Shupeng
authors.initials: S
authors.lastname: Gai
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Mu
authors.forename: Chunying
authors.initials: C
authors.lastname: Liu
authors.forename: Shao
authors.initials: S
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Guosheng
authors.initials: G
authors.lastname: Zheng
mesh_terms: D003080:Cold Temperature; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods; D018745:Genome, Plant; D018895:Microsatellite Repeats; D029596:Paeonia / Q000235:genetics*; D057445:Plant Dormancy / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods; D014157:Transcription Factors / Q000235:genetics; D014197:Trees / Q000235:genetics*
chemical_list: D014157:Transcription Factors