title: Glutamic acid decarboxylase immunoreactivity in the mossy fiber terminals of the hippocampus of genetic absence epileptic rats.
abstract: OBJECTIVE
  Genetic absence epilepsy rats from Strasbourg (GAERS) provide a model of     absence epilepsy. Although excessive GABA mediation within the     thalamo-cortico-thalamic circuit has been shown to play a role in absence     epilepsy, neuronal networks of hippocampus have recently received attention.     Glutamic acid decarboxylase (GAD) was previously shown to be increased after     convulsive seizures in the mossy fiber terminals (MFTs) of hippocampus. The aim     of the present study was to investigate whether the change in the level of this     enzyme in convulsive seizures is also observed in rats having genetic absence     epilepsy.
  
  
  METHODS
  Hippocampal CA3 and dentate regions were     processed for transmission electron microscopic evaluations. Thin sections were     incubated with anti-GAD65/67 antibody. The NIH Image Analysis program was used     for the quantitative analysis.
  
  
  RESULTS
  It was observed that GAD65/67     immunoreactivity was positive in CA3 and dentate gyrus MFTs of both groups and     the difference in the density of immunolabeling between the groups was not     statistically significant.
  
  
  CONCLUSIONS
  The present study demonstrated that GABA     synthesizing enzyme, GAD, is found in MFTs of Wistar and GAERS hippocampus and     this enzyme does not show an increase in these terminals in absence epilepsy, in     contrast to convulsive seizures.
authors.affiliation: Marmara University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey. ssirvanci@yahoo.com
authors.forename: Serap
authors.initials: S
authors.lastname: Sirvanci
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Canillioglu
authors.forename: Dilek
authors.initials: D
authors.lastname: Akakin
authors.forename: Sukru
authors.initials: S
authors.lastname: Midillioglu
authors.forename: Sercan Dogukan
authors.initials: SD
authors.lastname: Yildiz
authors.forename: Filiz
authors.initials: F
authors.lastname: Onat
authors.forename: Tangul
authors.initials: T
authors.lastname: San
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000201:enzymology / Q000648:ultrastructure; D018891:Dentate Gyrus / Q000201:enzymology / Q000648:ultrastructure; D004195:Disease Models, Animal; D004832:Epilepsy, Absence / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology* / Q000503:physiopathology / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron / Q000379:methods; D019599:Mossy Fibers, Hippocampal / Q000201:enzymology* / Q000648:ultrastructure; D009433:Neural Inhibition / Q000235:genetics; D017729:Presynaptic Terminals / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1; C401141:glutamate decarboxylase 2