title: Functional profile of cytomegalovirus (CMV)-specific CD8+ T cells and kinetics of NKG2C+ NK cells associated with the resolution of CMV DNAemia in allogeneic stem cell transplant recipients.
abstract: Immune mechanisms involved in control of cytomegalovirus (CMV) infection in the allogeneic stem cell transplantation setting have not been fully disclosed. CMV pp65 and IE-1-specific CD8(+) T cells expressing IFN-γ, TNF-α, and CD107a, alone or in combination, and NKG2C(+) NK cells were prospectively enumerated during 13 episodes of CMV DNAemia. The expansion of monofunctional and polyfunctional CD8(+) T cells was associated with CMV DNAemia clearance. The size and functional diversity of the expanding CD8(+) T-cell population was greater in self-resolved episodes than in episodes treated with antivirals. These differences were related to the magnitude of expansion of cognate antigen IFN-γ CD4(+) T cells. The resolution of CMV DNAemia was associated frequently with a marked expansion of both CD56(dim) /CD16(+) NK cells and NKG2C(+) CD56(bright) /CD16(-) NK cells. The data lend support to the role of polyfunctional CD8(+) T cells in controlling CMV replication in the allogeneic stem cell transplantation setting, and suggest that NKG2C(+) NK cells may be involved critically in the resolution of CMV DNAemia episodes.
authors.affiliation: Microbiology Service Hospital Clínico Universitario, Valencia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Muñoz-Cobo
authors.forename: Carlos
authors.initials: C
authors.lastname: Solano
authors.forename: Isabel
authors.initials: I
authors.lastname: Benet
authors.forename: Elisa
authors.initials: E
authors.lastname: Costa
authors.forename: María José
authors.initials: MJ
authors.lastname: Remigia
authors.forename: Rafael
authors.initials: R
authors.lastname: de la Cámara
authors.forename: José
authors.initials: J
authors.lastname: Nieto
authors.forename: Javier
authors.initials: J
authors.lastname: López
authors.forename: Paula
authors.initials: P
authors.lastname: Amat
authors.forename: Ana
authors.initials: A
authors.lastname: Garcia-Noblejas
authors.forename: Dayana
authors.initials: D
authors.lastname: Bravo
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Clari
authors.forename: David
authors.initials: D
authors.lastname: Navarro
mesh_terms: D000328:Adult; D019002:CD56 Antigen / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000821:virology; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D055654:NK Cell Lectin-Like Receptor Subfamily C / Q000276:immunology* / Q000378:metabolism; D010750:Phosphoproteins / Q000276:immunology; D017452:Receptors, IgG / Q000378:metabolism; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000276:immunology; D055815:Young Adult
chemical_list: D019002:CD56 Antigen; D004279:DNA, Viral; C527059:KLRC2 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; D055654:NK Cell Lectin-Like Receptor Subfamily C; D010750:Phosphoproteins; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D007371:Interferon-gamma