title: Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging.
abstract: A method is presented for acquiring high-spatial-resolution spectral maps, in particular for Raman micro-spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating the results. This method achieves up to 30 times reduction in the sampling time compared to raster-scanning, the resulting images have excellent correlation with conventional histopathological staining, and are achieved with sufficient spectral signal-to-noise ratio to identify individual tissue structures. The benefits of this selective sampling method are not limited to tissue imaging however; it is expected that the method may be applied to other techniques which employ point-by-point mapping of large substrates.
authors.affiliation: School of Physics and Astronomy, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Rowlands
authors.forename: Sandeep
authors.initials: S
authors.lastname: Varma
authors.forename: William
authors.initials: W
authors.lastname: Perkins
authors.forename: Iain
authors.initials: I
authors.lastname: Leach
authors.forename: Hywel
authors.initials: H
authors.lastname: Williams
authors.forename: Ioan
authors.initials: I
authors.lastname: Notingher
mesh_terms: D000465:Algorithms; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D012867:Skin / Q000166:cytology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
chemical_list: D011137:Polystyrenes