title: Fine-needle aspiration as a diagnostic technique in 50 cases of primary Ewing sarcoma/peripheral neuroectodermal tumor. Institut Curie's experience.
abstract: Fine-needle aspiration (FNA) followed by a core-needle biopsy during general anesthesia is a method for diagnosing pediatric tumors in our Institute. To complete the diagnosis in the case of round cell sarcomas, cytology material is also used for genomic analyses, that is, karyotyping and molecular biology-derived techniques. Fifty primary Ewing sarcomas/peripheral neuroectodermal tumors (ES/PNET) in 50 patients were sampled. Cytological diagnoses were "malignant" in all cases and accurate (ES/PNET) in 46 (92%). Two (4%) cases were misdiagnosed as neuroblastoma, and two others (4%) as rhabdomyosarcoma and nephroblastoma. No suspicious or false-negative results were rendered. Karyotyping was performed in 20 (40%) cases and was interpretable in 17 cases but not in three cases. Molecular search for ES/PNET fusion transcripts were performed in all cases and were detected in 44 (88%) cases, but not in six (12%) cases. In conclusion, FNA assisted by genomic techniques is powerful methods to accurate diagnose ES/PNET.
authors.affiliation: Department of Tumor Biology, Institut Curie, Paris, France. jerzy.klijanienko@curie.net
authors.forename: Jerzy
authors.initials: J
authors.lastname: Klijanienko
authors.forename: Jérôme
authors.initials: J
authors.lastname: Couturier
authors.forename: Franck
authors.initials: F
authors.lastname: Bourdeaut
authors.forename: Paul
authors.initials: P
authors.lastname: Fréneaux
authors.forename: Stelly
authors.initials: S
authors.lastname: Ballet
authors.forename: Hervé
authors.initials: H
authors.lastname: Brisse
authors.forename: Réal
authors.initials: R
authors.lastname: Lagacé
authors.forename: Olivier
authors.initials: O
authors.lastname: Delattre
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Pierron
authors.forename: Philippe
authors.initials: P
authors.lastname: Vielh
authors.forename: Xavier
authors.initials: X
authors.lastname: Sastre-Garau
authors.forename: Jean
authors.initials: J
authors.lastname: Michon
mesh_terms: D059786:Abnormal Karyotype; D000293:Adolescent; D000328:Adult; D044963:Biopsy, Fine-Needle; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007621:Karyotyping; D008297:Male; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000175:diagnosis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000175:diagnosis* / Q000235:genetics; D055815:Young Adult