title: [Detection of bovine respiratory syncytial virus by RT-PCR].
abstract: The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals.
authors.forename: A G
authors.initials: AG
authors.lastname: Glotov
authors.forename: T I
authors.initials: TI
authors.lastname: Glotova
authors.forename: I Ia
authors.initials: IIa
authors.lastname: Stroganova
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology; D009297:Nasal Mucosa / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000662:veterinary; D018112:Respiratory Syncytial Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D012800:Siberia
chemical_list: D012367:RNA, Viral