title: Antigen-loaded dissolving microneedle array as a novel tool for percutaneous vaccination.
abstract: Antigen-loaded dissolving microneedle array (dMNA) patches were investigated as novel systems for vaccine delivery into the skin, where immuno-competent dendritic cells are densely distributed. We fabricated micron-scale needles arrayed on patches, using chondroitin sulfate mixed with a model antigen, ovalbumin. Insertion of dMNA effectively delivered substantial amounts of ovalbumin into the skin within 3 min and induced robust antigen-specific antibody responses in the sera of mice. The antibody dose-response relationship showed that the efficiency of dMNA patch immunization was comparable to that of conventional intradermal injections. Thus, Antigen-loaded dMNA patches are a promising antigen-delivery system for percutaneous vaccination.
authors.affiliation: Division of Quality Assurance, National Institute of Infectious Diseases,4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan. snaito@nih.go.jp
authors.forename: Seishiro
authors.initials: S
authors.lastname: Naito
authors.forename: Yukako
authors.initials: Y
authors.lastname: Ito
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kiyohara
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kataoka
authors.forename: Masaki
authors.initials: M
authors.lastname: Ochiai
authors.forename: Kanji
authors.initials: K
authors.lastname: Takada
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000906:Antibodies / Q000097:blood; D004306:Dose-Response Relationship, Immunologic; D004864:Equipment and Supplies; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D000906:Antibodies; D010047:Ovalbumin