title: Downregulation of Dicer, a component of the microRNA machinery, in bladder             cancer.
abstract: Dysregulation of microRNA metabolism has been observed in a variety of human             cancers. In this study, we evaluated the expression of the enzymes of the machinery             Dicer, Drosha and DGCR8, in transitional cell carcinomas (TCCs) of the urinary             bladder. The expression of Dicer, Drosha and DGCR8 was analyzed using semi-quantitative             RT-PCR in clinical specimens from normal bladder mucosa, TCCs and their normal             adjacent tissues (NATs). Immunohistochemistry was performed to compare the expression             of Dicer in normal, TCCs and NATs. Our study demonstrated that Dicer mRNA levels             in TCCs were significantly lower compared to normal samples and NAT samples. The             immunohistochemistry results revealed that Dicer protein levels in TCCs were significantly             downregulated compared to normal bladder mucosa and NATs. Our data demonstrated             that Dicer is significantly downregulated in TCCs compared to paired NAT samples             and normal samples, suggesting that reduced expression of Dicer may play an important             role in bladder cancer.
authors.affiliation: Department of Urology, The Fourth Affiliated Hospital of Nantong Medical College, Yancheng City No 1 People's Hospital, Nanjing, Jiangsu, PR China.
authors.forename: Deyao
authors.initials: D
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Pengchao
authors.initials: P
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000201:enzymology* / Q000473:pathology; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology
chemical_list: C474043:DGCR8 protein, human; D035683:MicroRNAs; D011506:Proteins; D016601:RNA-Binding Proteins; C408087:DICER1 protein, human; C517918:DROSHA protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases