title: Effect of enamel matrix derivative protein on the healing of standardized epithelial wounds: a histomorphometric analysis in vivo.
abstract: The enamel matrix derivative (EMD) is a preparation of the enamel matrix proteins secreted by the Hertwig's epithelial root sheath. It has been shown that EMD promotes periodontal wound healing; however, the significance of the protein in repairing skin wounds is insufficiently addressed. The aim of this in vivo histomorphometric investigation was to analyse the effect of EMD protein on the healing of standardised epithelial wounds. Dorsal skin of 22-week-old female guinea pigs (n = 33) was scarified and divided into test- (topical application of EMD) and control-sites (sutured and allowed to heal). Animals were euthanised at specific time intervals and the specimens were then evaluated histomorphometrically. The mean widths of the external wound gaps (WGs) in the test- and control-sites at the 5th, 20th and 35th day of healing were 5·89, 3·6 and 1·01 mm and 6·41, 5·02 and 3·43 mm, respectively. Histomorphometric analysis showed a statistically significant difference in the WGs between the test- and control-sites. A significant increase in the formation of organised connective tissue matrix, collagen fibres and early muscle formation was observed in the test-sites as compared with the control-sites. Within the limits of this study, it is concluded that topical application of the EMD on standardised epithelial allows early wound closure and promotes healing as compared to when the defects are merely sutured.
authors.affiliation: Growth Factors and Bone Regeneration, Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, PO Box 103851. Riyadh 11616, Saudi Arabia. hezaimik16@gmail.com
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Hezaimi
authors.forename: Mansour
authors.initials: M
authors.lastname: Al-Askar
authors.forename: Hamad
authors.initials: H
authors.lastname: Al-Fahad
authors.forename: Abdulaziz
authors.initials: A
authors.lastname: Al-Rasheed
authors.forename: Nabil
authors.initials: N
authors.lastname: Al-Sourani
authors.forename: Terrence
authors.initials: T
authors.lastname: Griffin
authors.forename: Rory
authors.initials: R
authors.lastname: O'Neill
authors.forename: Fawad
authors.initials: F
authors.lastname: Javed
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D004195:Disease Models, Animal; D004848:Epithelium / Q000293:injuries / Q000473:pathology; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D015601:Injury Severity Score; D011897:Random Allocation; D012016:Reference Values; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000473:pathology*
chemical_list: D003746:Dental Enamel Proteins; C109069:enamel matrix proteins