title: 9-methoxycamptothecin from Nothapodytes foetida induces apoptosis in murine sarcoma S180 cells.
abstract: 9-Methoxycamptothecin (MCPT) was found to have antitumour activities through topoisomerase inhibition. However, the type of cell death induced in the tumour cells treated with MCPT was not elucidated. In this study, MCPT and camptothecin were isolated from Nothapodytes foetida distributed in Hubei Province, China and identified by NMR spectroscopy. MCPT was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay using camptothecin as reference. Annexin V-FITC/propidium iodide double staining and real-time PCR were also performed. The IC50 value was (0.385 +/- 0.08) microM. The apoptosis rates increased from 9.5% to 17.27%, 30.14%, and 66.46% with an increase in MCPT concentrations from 0, 0.19, 0.38, to 0.95 microM, respectively. The ratio of Bax/Bcl-2 also increased from 1 to 1.61, 2.43, and 4.57, respectively. Bax and Bcl-2 are crucial to the mitochondria pathway. The results indicate that the mitochondria pathway may be involed in MCPT-induced murine sarcoma S180 apoptosis.
authors.affiliation: Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liao
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Mingzhang
authors.initials: M
authors.lastname: Ao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yueyuan
authors.initials: Y
authors.lastname: Shi
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D017931:DNA Primers; D005434:Flow Cytometry; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D012513:Sarcoma, Experimental / Q000473:pathology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D017931:DNA Primers; C025724:9-methoxycamptothecin; D002166:Camptothecin