title: Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples.
abstract: A compact portable chemiluminescent biosensor for simple, rapid, and ultrasensitive on-site quantification of fumonisins (fumonisin B1+fumonisin B2) in maize has been developed. The biosensor integrates a competitive lateral flow immunoassay based on enzyme-catalyzed chemiluminescence detection and a highly sensitive portable charge-coupled device (CCD) camera, employed in a contact imaging configuration. The use of chemiluminescence detection allowed accurate and objective analyte quantification, rather than qualitative or semi-quantitative information usually obtained employing conventional lateral flow immunoassays based on colloidal gold labeling. A limit of detection of 2.5 μgL(-1) for fumonisins was achieved, with an analytical working range of 2.5-500 μgL(-1) (corresponding to 25-5000 μgkg(-1) in maize flour samples, according to the extraction procedure). Total assay time was 25 min, including sample preparation. A simple and convenient extraction procedure, performed by suspending the sample in a buffered solution and rapidly heating to eliminate endogenous peroxidase enzyme activity was employed for maize flour samples analysis, obtaining recoveries in the range 90-115%, when compared with LC-MS/MS analysis. The chemiluminescence immunochromatography-based biosensor is a rapid, low cost portable test suitable for point-of-use applications.
authors.affiliation: Department of Pharmaceutical Sciences, Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Mirasoli
authors.forename: Angela
authors.initials: A
authors.lastname: Buragina
authors.forename: Luisa Stella
authors.initials: LS
authors.lastname: Dolci
authors.forename: Patrizia
authors.initials: P
authors.lastname: Simoni
authors.forename: Laura
authors.initials: L
authors.lastname: Anfossi
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Giraudi
authors.forename: Aldo
authors.initials: A
authors.lastname: Roda
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D037341:Fumonisins / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins