title: [Effect of 5-Aza-CdR on biological activity and inhibitor of DNA binding 4 gene expression in human erythroleukemia cell line K562].
abstract: This study was aimed to investigate the effect of 5-Aza-CdR on the biological activity of human erythroleukemia cell line K562 and the expression of inhibitor of DNA binding 4 (ID4). ID4 methylation in K562 cell line was detected by methylation-specific PCR. RQ-PCR was used to analyze the expression levels of ID4 mRNA in K562 cell line treated by different concentrations of 5-Aza-CdR. Cell apoptosis rate and cell cycle were analyzed by flow cytometry. The result showed that ID4 gene methylation existed in K562 cells, ID4 mRNA expression in K562 cells treated with 5-Aza-CdR increased in a concentration-dependent manner, the difference between experimental groups was statistical significant (p < 0.01). The 5-Aza-CdR could enhance the apoptotic rate of K562 cells in time and dose-dependent manner, the apoptotic rate of K562 cells highly correlated to relative expression level of ID4 mRNA (r = 0.95). After the K562 cells were treated by 5-Aza-CdR for 48 hours, cells in G(0)/G(1) phase increased, cells in G(2)/M phase decreased along with enhancement of drug concentration. It is concluded that methyltransferase inhibitor 5-Aza-CdR can re-express the silent ID4 gene in K562 cells. The upregulation of ID4 may be a key factor to give rise to cell apoptosis, and the cell cycle of K562 cells can be arrested by 5-Aza-CdR.
authors.affiliation: Department of Hematology, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Chun
authors.initials: C
authors.lastname: Huang
authors.forename: Chun-Rui
authors.initials: CR
authors.lastname: Li
authors.forename: Deng-Ju
authors.initials: DJ
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects; D019175:DNA Methylation; D000077209:Decitabine; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics*; D020014:K562 Cells
chemical_list: C490128:ID4 protein, human; D051796:Inhibitor of Differentiation Proteins; D000077209:Decitabine; D001374:Azacitidine