title: A new pentameric structure of rotavirus NSP4 revealed by molecular replacement.
abstract: The region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 has been purified and crystallized and diffraction data have been collected to a resolution of 2.6 Å. Several attempts to solve the structure by the molecular-replacement method using the available tetrameric structures of this domain were unsuccessful despite a sequence identity of 73% to the already known structures. A more systematic approach with a dimer as the search model led to an unexpected pentameric structure using the program Phaser. The various steps involved in arriving at this molecular-replacement solution, which unravelled a case of subtle variation between different oligomeric states unknown at the time of solving the structure, are presented in this paper.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka, India.
authors.forename: Anita R
authors.initials: AR
authors.lastname: Chacko
authors.forename: J
authors.initials: J
authors.lastname: Jeyakanthan
authors.forename: G
authors.initials: G
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Sekar
authors.forename: C Durga
authors.initials: CD
authors.lastname: Rao
authors.forename: Eleanor J
authors.initials: EJ
authors.lastname: Dodson
authors.forename: Kaza
authors.initials: K
authors.lastname: Suguna
authors.forename: Randy J
authors.initials: RJ
authors.lastname: Read
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020836:Protein Structure, Quaternary; D012401:Rotavirus / Q000737:chemistry*; D016415:Sequence Alignment; D014118:Toxins, Biological / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins