title: Characterization of 12  microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae),  and cross-amplification in five other lamprey species.
abstract: The  Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is  of conservation concern in North America and Asia. Data on Pacific lamprey  population structure are scarce and conflicting, impeding conservation efforts.  We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three  to 13 alleles per locus were observed in a sample of 51 fish collected from the  West Fork Illinois River, Oregon. Observed heterozygosity ranged from 0.235 to  0.902 and expected heterozygosity ranged from 0.214 to 0.750. Cross-species  amplification produced 8 to 12 polymorphic loci in four other Entosphenus species  and in the western brook lamprey (Lampetra richardsoni). Two loci appear  to be diagnostic for distinguishing Entosphenus from Lampetra.  These markers will be valuable for evaluating population structure and making  conservation decisions for E. tridentatus and other lamprey species.
authors.affiliation: Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: E K
authors.initials: EK
authors.lastname: Spice
authors.forename: T A
authors.initials: TA
authors.lastname: Whitesel
authors.forename: C T
authors.initials: CT
authors.lastname: McFarlane
authors.forename: M F
authors.initials: MF
authors.lastname: Docker
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D056426:Genetic Loci; D005828:Genetics, Population; D015698:Genomic Library; D060005:Genotyping Techniques; D006579:Heterozygote; D007798:Lampreys / Q000235:genetics*; D018895:Microsatellite Repeats; D009922:Oregon; D011110:Polymorphism, Genetic; D045483:Rivers; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004247:DNA