title: Pro-inflammatory functions of carp CXCL8-like and CXCb chemokines.
abstract: Numerous CXC chemokines have been identified in fish, however, their role in inflammation is not well established. Here, CXC chemokines of the CXCL8-like (CXCa_L1 and CXCL8_L2) and CXCL9/10/11-like (CXCb) subset were investigated in carp. Recombinant CXCa_L1, CXCL8_L2 and CXCb all stimulated chemotaxis of macrophages and granulocytes in vitro. CXCb also attracted lymphocytes. Distinct effects on phagocyte activation were observed: the CXCL8-like chemokines increase respiratory burst activity, but not nitrite production. The three chemokines differentially induced a moderate increase in IL-1β, CXCa_L1 and CXCL8_L2 gene expression. Intracellular calcium mobilization in granulocytes upon CXCa_L1 stimulation implies signal transduction through G-protein coupled CXC receptors. Notably, upon intraperitoneal administration, carp CXCL8-like chemokines strongly induced in vivo leukocyte recruitment, including neutrophils and monocytes/macrophages, in contrast to CXCb, for which the number of recruited leukocytes was low. The results indicate functional homology for carp CXCL8-like and CXCb chemokines with mammalian CXCL8 and CXCL9-11, respectively.
authors.affiliation: Cell Biology and Immunology Group, Dept. of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
authors.forename: Lieke M
authors.initials: LM
authors.lastname: van der Aa
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chadzinska
authors.forename: Lieke A
authors.initials: LA
authors.lastname: Golbach
authors.forename: Carla M S
authors.initials: CM
authors.lastname: Ribeiro
authors.forename: B M
authors.initials: BM
authors.lastname: Lidy Verburg-van Kemenade
mesh_terms: D000818:Animals; D002347:Carps / Q000276:immunology*; D019743:Chemokines, CXC / Q000276:immunology*; D029941:Fish Proteins / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology; D060226:Head Kidney / Q000166:cytology; D008264:Macrophages / Q000276:immunology
chemical_list: D019743:Chemokines, CXC; D029941:Fish Proteins