title: Biological activity is the likely origin of the intersection between the photoreceptor inner and outer segments of the rat retina as determined by optical coherence tomography.
abstract: BACKGROUND
  Recent research on macular diseases has prompted investigations into the condition of the intersection between the photoreceptor inner and outer segments (IS/OS) and the relationship with retinal photoreceptor abnormalities. Although the origin of the IS/OS in optical coherence tomography (OCT) images is unclear, it may be related to either the cellular activity of the photoreceptors or the structure of the OS disks. To address this question, we compared the IS/OS status in OCT images of rat retinas before and after euthanasia.
  
  
  METHODS
  OCT images were taken before and after euthanasia in four eyes of two Brown Norway rats. After the OCT images were taken, the rats were used for histopathological studies to confirm that retinal structures were intact.
  
  
  RESULTS
  Before euthanasia, the IS/OS and external limiting membrane (ELM) line were clearly identifiable on the OCT images. However, after euthanasia, neither the IS/OS nor the ELM line was evident in three out of four eyes, and a faint IS/OS and an ELM line were identified in one eye. Histopathological analysis did not show any abnormalities in the retina in any of the four eyes.
  
  
  CONCLUSIONS
  The origin of the IS/OS identified in OCT images is likely related to the biological activities of the photoreceptor cells.
authors.affiliation: Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. phthisis@nifty.com
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yagi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Usui
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kimura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Agawa
authors.forename: Rintaro
authors.initials: R
authors.lastname: Tsukahara
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yamakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Goto