title: Macro histone H2A1.2 (macroH2A1) protein suppresses mitotic kinase VRK1 during interphase.
abstract: VRK1-mediated phosphorylation of histone H3 should be restricted in mitosis for consistent cell cycling, and defects in this process trigger cellular catastrophe. However, an interphasic regulator against VRK1 has not been actually investigated so far. Here, we show that the histone variant macrodomain-containing histone H2A1.2 functions as a suppressor against VRK1 during interphase. The level of macroH2A1.2 was markedly reduced in the mitotic phase, and the macroH2A1.2-mediated inhibition of histone H3 phosphorylation occurred mainly during interphase. We also found direct interaction and binding features between VRK1 and macroH2A1.2 by NMR spectroscopy. Hence, our findings might provide valuable insight into the underlying molecular mechanism regarding an epigenetic regulation of histone H3 during the cell cycle.
authors.affiliation: Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Wanil
authors.initials: W
authors.lastname: Kim
authors.forename: Goutam
authors.initials: G
authors.lastname: Chakraborty
authors.forename: Sangjune
authors.initials: S
authors.lastname: Kim
authors.forename: Joon
authors.initials: J
authors.lastname: Shin
authors.forename: Choon-Ho
authors.initials: CH
authors.lastname: Park
authors.forename: Min-Woo
authors.initials: MW
authors.lastname: Jeong
authors.forename: Nagakumar
authors.initials: N
authors.lastname: Bharatham
authors.forename: Ho Sup
authors.initials: HS
authors.lastname: Yoon
authors.forename: Kyong-Tai
authors.initials: KT
authors.lastname: Kim
mesh_terms: D057809:HEK293 Cells; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007399:Interphase; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis; D008958:Models, Molecular; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport
chemical_list: D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; C120756:macroH2A histone; D017346:Protein Serine-Threonine Kinases; C109890:VRK1 protein, human