title: Protective effects of polysaccharides from soybean meal against X-ray radiation induced damage in mouse spleen lymphocytes.
abstract: The aim of this study was to investigate radioprotective effect of the polysaccharides from soybean meal (SMP) against X-ray radiation-induced damage in mouse spleen lymphocytes. MTT and comet assay were performed to evaluate SMP's ability to prevent cell death and DNA damage induced by radiation. The results show that, X-ray radiation (30 KV, 10 mA, 8 min (4 Gy)) can significantly increase cell death and DNA fragmentation of mouse spleen lymphocytes. Pretreatment with SMP for 2 h before radiation could increase cell viability, moreover, the SMP can reduce X-ray radiation-induced DNA damage. The percentage of tail DNA and the tail moment of the SMP groups were significantly lower than those of the radiation alone group (p < 0.05). These results suggest SMP may be a good candidate as a radioprotective agent.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China; E-Mails:  yaoleiyl2000@163.com  (L.Y.)  zhaoht9999@163.com  (H.Z.);  ccuilin@hit.edu.cn  (C.C.);  yangxin940@163.com  (X.Y.).
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Haitian
authors.initials: H
authors.lastname: Zhao
authors.forename: Cuilin
authors.initials: C
authors.lastname: Cheng
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Fu
authors.forename: Jiaren
authors.initials: J
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002470:Cell Survival; D020552:Comet Assay; D004249:DNA Damage; D053938:DNA Fragmentation; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects* / Q000528:radiation effects; D014965:X-Rays / Q000009:adverse effects*
chemical_list: D011134:Polysaccharides; D011837:Radiation-Protective Agents