title: Functional characterization of a Chrysanthemum dichrum stress-related promoter.
abstract: A 1,474-bp stress-inducible CdDREBa promoter was identified from Chrysanthemum dichrum, revealing several candidate stress-related cis-acting elements (MYC-box, MYB site, GT-1, and W-box) within it. In Arabidopsis leaf tissues transformed with a CdDREBa promoter-β-glucuronidase (GUS) gene fusion, serially 5'-deleted CdDREBa promoters were differentially activated by cold and salinity. Histochemical and quantitative assays of GUS expression allowed us to localize a critical part of the promoter located between upstream 430 and 351 nt. This 80-bp fragment enhanced GUS expression under salinity stress when fused to -90/+8 CaMV 35S minimal promoter. Further promoter internal-deletion assays indicated that a low temperature-responsive element was located between positions -430 and -390, and a salinity inducible one between -385 and -351. Our results showed that there was a novel stress-related critical region except for the known cis-acting element (MYC-box, GT-1) in CdDREBa promoter.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.forename: Pirui
authors.initials: P
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Chang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D031202:Chrysanthemum / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D012965:Sodium Chloride; D005966:Glucuronidase