title: OmpR may regulate the putative YehU/YehT two-component system in Salmonella enterica serovar Typhi under hypotonic growth condition.
abstract: Decreased expression (twofold) of a putative yehUTS operon of which yehUT encodes a putative YehU/YehT two-component system in the ompR mutant from Salmonella enterica serovar Typhi (S. Typhi) GIFU10007 under hypotonic growth condition was observed by qRT-PCR. Purified recombinant protein OmpR(His6) of GIFU10007 was shown to bind the upstream region of the yehU gene by the gel-shift assay. In addition, the yehT deletion mutant (ΔyehT) displayed differential expression (twofold or higher) of 26 genes under the condition by the DNA microarray analysis. Altogether, OmpR might regulate the YehUT system in S. Typhi under hypotonic growth condition.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu, China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Du
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ji
authors.forename: Bin
authors.initials: B
authors.lastname: Ni
authors.forename: Linxiang
authors.initials: L
authors.lastname: Mao
authors.forename: Shungao
authors.initials: S
authors.lastname: Xu
authors.forename: Xiumei
authors.initials: X
authors.lastname: Sheng
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Huang
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007038:Hypotonic Solutions / Q000378:metabolism; D046228:Microarray Analysis; D009997:Osmotic Pressure; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D060888:Real-Time Polymerase Chain Reaction; D012485:Salmonella typhi / Q000502:physiology*; D015398:Signal Transduction; D013312:Stress, Physiological; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007038:Hypotonic Solutions; D015534:Trans-Activators; C112177:osmolarity response regulator proteins