title: Morphology and infectivity of virus that persistently caused infection in an AGS cell line.
abstract: A recent report has indicated that proteins and genes of simian virus 5 (SV5) are detected in a human gastric adenocarcinoma (AGS) cell line, which is widely provided for oncology, immunology, and microbiology research. However, the production of infective virions has not been determined in this cell line. In this study, the morphology and infectivity of the virus particles of the AGS cell line were studied by light and electron microscopy and virus transmission assay. The virus particles were approximately 176.0 ± 41.1 nm in diameter. The particles possessed projections 8-12 nm long on the surface and contained a nucleocapsid determined to be 13-18 nm in width and less than 1,000 nm in length. The virus was transmissible to the Vero cell line, induced multinuclear giant cell formation, and reproduced the same shape of antigenic virions. In this study, the persistently infected virus in the AGS cell line was determined to be infective and form reproducible virions, and a new morphological feature of SV5 was determined.
authors.affiliation: Department of Microbiology and Infection Control, Osaka Medical College, Daigaku-machi, Takatsuki, Osaka, Japan.
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Ooi
authors.forename: Eriko
authors.initials: E
authors.lastname: Daikoku
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Aoki
authors.forename: Chizuko
authors.initials: C
authors.lastname: Morita
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kohno
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sano
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000494:pharmacology; D002460:Cell Line / Q000821:virology*; D048430:Cell Shape / Q000187:drug effects; D016480:Helicobacter pylori / Q000502:physiology; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D045402:Parainfluenza Virus 5 / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure*; D011817:Rabbits; D019351:Rubulavirus Infections / Q000635:transmission / Q000821:virology*; D014771:Virion / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure*
chemical_list: D000914:Antibodies, Viral