title: Isolation and characterization of microsatellite loci in Pistacia weinmannifolia (Anacardiaceae).
abstract: Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (H(o)) and expected (H(e)) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; E-Mail:  chenst@kib.ac.
authors.forename: Shaotian
authors.initials: S
authors.lastname: Chen
authors.forename: Xiayu
authors.initials: X
authors.lastname: Wu
authors.forename: Yunheng
authors.initials: Y
authors.lastname: Ji
authors.forename: Junbo
authors.initials: J
authors.lastname: Yang
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D005828:Genetics, Population; D005838:Genotype; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D027927:Pistacia / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant