title: Microfluidic capture and release of bacteria in a conical nanopore array.
abstract: We present a simple and inexpensive method for the capture and release of bacteria contained in an array of conical nanopores on a membrane inside a microfluidic device. As an example, we demonstrate that cyanobacteria can be captured, one bacterium per pore, in a defined orientation with over 500 bacteria per membrane with viabilities as high as 100%. The device can also specifically capture cyanobacteria from a mixed suspension of cyanobacteria and chlamydomonas with a selectivity as high as 90%.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Eric W
authors.initials: EW
authors.lastname: Hall
authors.forename: Romana
authors.initials: R
authors.lastname: Schirhagl
authors.forename: Hitomi
authors.initials: H
authors.lastname: Mukaibo
authors.forename: Charles R
authors.initials: CR
authors.lastname: Martin
authors.forename: Richard N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D000458:Cyanobacteria / Q000302:isolation & purification*; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation*; D058608:Nanopores; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D016062:Porosity