title: Resistance to  lipopolysaccharide-induced  endotoxic shock in heterozygous Zfp191  gene-knockout mice.
abstract: Zinc  finger protein 191, ZNF24 and Zfp191 in both humans and mice belong to the SCAN  domain subfamily of Krüppel-like zinc finger transcription factors. Previous  studies have suggested that Zfp191 is a pleiotropic factor involved in  embryonic development, hematopoiesis and tumorigenesis. However, little is  known about its target genes or its role in other physiological and  pathological processes. We have identified the putative target genes of Zfp191,  using an in silico genome-wide scan. Three hundred and fifty-five  putative target genes were identified, which were enriched into the pathways of  immune response according to the pathway analysis. These targets indicated that  Zfp191 may function as a mediator of the immune response. This was verified in  mice heterozygous for Zfp191 (Zfp191(+/-)) using  a lipopolysaccharide (LPS)-induced endotoxic shock model. After LPS injection,  Zfp191(+/-) mice produced significantly less IL-1β and IL-6 compared to wild-type  mice and were resistant to LPS-induced endotoxic shock. The loss of Zfp191 may  suppress systemic inflammation by reducing these cytokine levels during  LPS-induced endotoxic shock.
authors.affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: R L
authors.initials: RL
authors.lastname: Sun
authors.forename: H Y
authors.initials: HY
authors.lastname: Wang
authors.forename: X Y
authors.initials: XY
authors.lastname: Yang
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Sheng
authors.forename: L M
authors.initials: LM
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Fei
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics*; D019295:Computational Biology; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology*; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D006579:Heterozygote; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012772:Shock, Septic / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D016019:Survival Analysis
chemical_list: D002352:Carrier Proteins; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; C474810:Zfp24 protein, mouse