title: Detection of Helicobacter pylori DNA in preliminary stage gastric cancer cells.
abstract: OBJECTIVE
  Helicobacter pylori (HP) DNA was assayed using a newly fabricated voltammetric handheld circuit equipped with an interfaced graphite pencil electrode (PE) sensor.
  
  
  METHODS
  Under optimum analytical parameters, the square-wave (SW) stripping detection range attained was 0.1-0.7 mg/L.
  
  
  RESULTS
  A relative standard deviation of 1.68% (RSD, n = 5) was observed at a 3.2 mg/L HP DNA within a 90 s accumulation time. Under these conditions, the analytical detection limit approached 0.07 mg/L.
  
  
  CONCLUSIONS
  The developed methods can be applied to HP assays of preliminary stage gastric cancer patient tissues.
authors.affiliation: Biosensor Research Institute, Seoul National University of Science and Technology, Seoul, Korea.
authors.forename: Suw Young
authors.initials: SY
authors.lastname: Ly
authors.forename: So Dam
authors.initials: SD
authors.lastname: Yoo
authors.forename: Seung Kyu
authors.initials: SK
authors.lastname: Chun
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D019937:Diagnostic Techniques and Procedures; D042241:Early Diagnosis; D004553:Electric Conductivity; D004566:Electrodes; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D057230:Limit of Detection; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial