title: Sequence determination and analysis of the NSs genes of two tospoviruses.
abstract: The tospoviruses groundnut ringspot virus (GRSV) and zucchini lethal chlorosis virus (ZLCV) cause severe losses in many crops, especially in solanaceous and cucurbit species. In this study, the non-structural NSs gene and the 5'UTRs of these two biologically distinct tospoviruses were cloned and sequenced. The NSs sequence of GRSV and ZLCV were both 1,404 nucleotides long. Pairwise comparison showed that the NSs amino acid sequence of GRSV shared 69.6% identity with that of ZLCV and 75.9% identity with that of TSWV, while the NSs sequence of ZLCV and TSWV shared 67.9% identity. Phylogenetic analysis based on NSs sequences confirmed that these viruses cluster in the American clade.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Brasilia, DF 70910-900, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Hallwass
authors.forename: Mikhail O
authors.initials: MO
authors.lastname: Leastro
authors.forename: Mirtes F
authors.initials: MF
authors.lastname: Lima
authors.forename: Alice K
authors.initials: AK
authors.lastname: Inoue-Nagata
authors.forename: Renato O
authors.initials: RO
authors.lastname: Resende
mesh_terms: D020121:5' Untranslated Regions; D003001:Cloning, Molecular; D016000:Cluster Analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017865:Tospovirus / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins