title: An oligopeptide ligand-mediated therapeutic gene nanocomplex for liver cancer-targeted therapy.
abstract: The epidermal growth factor receptor (EGFR) is over-expressed in a wide variety of epithelial-derived cancer cells. In this study, EGFR-targeted gene carriers were designed to complex the therapeutic acetylcholinesterase gene (AChE gene), which suppresses cell proliferation via inactivating mitogen-activated protein kinase and PI3K/Akt pathways in cells, for treatment of EGFR-positive liver cancers. Different amounts of target ligand YC21 (an oligopeptide composed of 21 amino acid units) were coupled with the PEI(600)-CD (PC) vectors composed of β-cyclodextrin (β-CD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form the EGFR-targeted gene vectors (termed as YPCs). The YPC vectors possessed the highly efficient gene delivery ability to the EGFR-positive liver cancer cells. YPCs could effectively promote AChE gene expression. The YPC/AChE complexes produced excellent gene transfection abilities in EGFR-positive liver cancer cells in vitro and in vivo.
authors.affiliation: Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, PR China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
authors.forename: L H
authors.initials: LH
authors.lastname: Lai
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Wang
authors.forename: G P
authors.initials: GP
authors.lastname: Tang
authors.forename: W T
authors.initials: WT
authors.lastname: Yang
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000627:therapeutic use*; D011094:Polyethyleneimine / Q000737:chemistry; D014162:Transfection / Q000379:methods
chemical_list: D008024:Ligands; D009842:Oligopeptides; D011094:Polyethyleneimine; D066246:ErbB Receptors; D000110:Acetylcholinesterase