title: Novel oncogenic function of mesoderm development candidate 1 and its             regulation by MiR-574-3p in bladder cancer cell lines.
abstract: Our previous studies suggested that microRNA (miR)-574-3p is a candidate             tumor suppressor microRNA (miRNA) in human bladder cancer (BC). Among 17 down-regulated             miRNAs, miR-574-3p is located on chromosome 4p14 where we had identified a chromosomal             loss region by array-CGH in BC cell lines. MiR-574-3p expression was down-regulated             in BC cell lines. Gain-of-function analysis revealed that cell proliferation,             migration and invasion were significantly inhibited in miR‑574‑3p-transfected             BC cell lines. Flow cytometry analysis showed that cell apoptosis was induced             in miR-574-3p transfectants. Oligo microarray analysis suggested that the mesoderm             development candidate 1 (MESDC1) gene was a target gene in miR-574-3p transfectants.             Luciferase assays revealed that miR‑574‑3p was directly bound to MESDC1 mRNA.             MESDC1 is predicted to be a novel actin-binding protein located on chromosome             15q13. Although the gene is conserved among many species, its functional role             is still unknown in both human malignancies and normal tissues. Loss-of-function             studies demonstrated that cell proliferation, migration and invasion were significantly             inhibited in si-MESDC1-transfected BC cell lines. Flow cytometry analysis showed             that apoptosis was induced in si-MESDC1 transfectants. We are the first to demonstrate             that miR-574-3p is a miRNA with tumor suppressor function and that MESDC1 (which             has a potential oncogenic function in BC) may be targeted by miR-574-3p.
authors.affiliation: Department of Urology, Graduate School of Medical and Dental                     Sciences, Kagoshima University, Kagoshima, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Tatarano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chiyomaru
authors.forename: Kazumori
authors.initials: K
authors.lastname: Kawakami
authors.forename: Hideki
authors.initials: H
authors.lastname: Enokida
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshino
authors.forename: Hideo
authors.initials: H
authors.lastname: Hidaka
authors.forename: Nijiro
authors.initials: N
authors.lastname: Nohata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Takenari
authors.initials: T
authors.lastname: Gotanda
authors.forename: Tokushi
authors.initials: T
authors.lastname: Tachiwada
authors.forename: Naohiko
authors.initials: N
authors.lastname: Seki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakagawa
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D018832:Molecular Chaperones / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C558811:MIRN574 microRNA, human; D035683:MicroRNAs; D018832:Molecular Chaperones; D012333:RNA, Messenger; C574664:TLNRD1 protein, human