title: Self-assembly of amphiphilic glycoconjugates into lectin-adhesive nanoparticles.
abstract: This work describes the synthesis and self-assembly of carbohydrate-clicked rod-coil amphiphilic systems. Copper-catalyzed Huisgen cycloaddition was efficiently employed to functionalize the hydrophilic extremity of PEG-b-tetra(p-phenylene) conjugates by lactose and N-acetyl-glucosamine ligands. The resulting amphiphilic systems spontaneously self-assembled into nanoparticles when dissolved in aqueous media, as evidenced by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small-angle X-ray scattering (SAXS). The formation of highly monodisperse micelles having a mean diameter of 10 nm was observed for systems containing a PEG 900 core, and a decrease in the hydrophilic moiety (PEG 600) led to the formation of vesicles with a broader size distribution. The presence of carbohydrate residues on the surfaces of the micelles and their ability to establish specific interactions with wheat germ agglutinin (WGA) and peanut agglutinin (PNA) were further highlighted by light-scattering measurements, thus confirming the attractive applications of such sugar micelles in biosensor devices.
authors.affiliation: Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP 53, F-38041 Grenoble Cedex 9, France.
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Dal Bó
authors.forename: Valdir
authors.initials: V
authors.lastname: Soldi
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Giacomelli
authors.forename: Christophe
authors.initials: C
authors.lastname: Travelet
authors.forename: Bruno
authors.initials: B
authors.lastname: Jean
authors.forename: Isabelle
authors.initials: I
authors.lastname: Pignot-Paintrand
authors.forename: Redouane
authors.initials: R
authors.lastname: Borsali
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fort
mesh_terms: D000269:Adhesives; D006001:Glycoconjugates / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D000269:Adhesives; D006001:Glycoconjugates; D037102:Lectins