title: Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole.
abstract: To develop a covalent and specific labeling method for single- and double-stranded plasmid DNA, photoresponsive oligonucleotide containing 3-cyanovinylcarbazole nucleoside was adopted. Single- and double-stranded plasmid DNA was successfully labeled/de-labeled with Cy3 and/or biotin by photoirradiation.
authors.affiliation: School of Materials Science, Japan Advanced institute of Science and Technology, Nomi, Ishikawa, Japan. kenzo@jaist.ac.jp
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Hiratsuka-Konishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ohtake
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Shinohara
authors.forename: Yoshinaga
authors.initials: Y
authors.lastname: Yoshimura
mesh_terms: D000181:Acrylonitrile / Q000031:analogs & derivatives / Q000737:chemistry; D002227:Carbazoles / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D055668:Photochemical Processes; D010957:Plasmids / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002227:Carbazoles; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA; D000181:Acrylonitrile