title: Cellulose synthase interactive protein 1 (CSI1) links microtubules and cellulose synthase complexes.
abstract: Cellulose synthase (CESA) complexes can be observed by live-cell imaging to move with trajectories that parallel the underlying cortical microtubules. Here we report that CESA interactive protein 1 (CSI1) is a microtubule-associated protein that bridges CESA complexes and cortical microtubules. Simultaneous in vivo imaging of CSI1, CESA complexes, and microtubules demonstrates that the association of CESA complexes and cortical microtubules is dependent on CSI1. CSI1 directly binds to microtubules as demonstrated by in vitro microtubule-binding assay.
authors.affiliation: Center for LignoCellulose Structure and Formation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Shundai
authors.initials: S
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Chris R
authors.initials: CR
authors.lastname: Somerville
authors.forename: Ying
authors.initials: Y
authors.lastname: Gu
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000494:pharmacology; D005964:Glucosyltransferases / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D013424:Sulfanilamides / Q000494:pharmacology; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; C551801:CSI1 protein, Arabidopsis; D002352:Carrier Proteins; D004136:Dinitrobenzenes; D009097:Multienzyme Complexes; D013424:Sulfanilamides; C012465:oryzalin; D005964:Glucosyltransferases; C478648:cellulose synthase