title: Non-invasive long-term and real-time analysis of endocrine cells on micro-electrode arrays.
abstract: Non-invasive high-throughput and long-term monitoring of endocrine cells is important for drug research, phenotyping, tissue engineering and pre-transplantation quality control. Here we report a novel approach to obtain simultaneous long-term electrical recordings of different islet cell types using multi-electrode arrays. We implemented wavelet transforms to resolve the low signal/noise ratio inherent to these measurements and extracted on-line a signature specific of cell activity. The architecture employed allows multiplexing a large number of electrodes for high-throughput screening. This method should be of considerable advantage in endocrine research and may be extended to other excitable cells previously not accessible to the technique.
authors.affiliation: Université de Bordeaux, CBMN, UMR 5248, F-33600 Pessac, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Raoux
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bornat
authors.forename: Adam
authors.initials: A
authors.lastname: Quotb
authors.forename: Bogdan
authors.initials: B
authors.lastname: Catargi
authors.forename: Sylvie
authors.initials: S
authors.lastname: Renaud
authors.forename: Jochen
authors.initials: J
authors.lastname: Lang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D007515:Islets of Langerhans / Q000502:physiology*; D051379:Mice; D008839:Microelectrodes; D018408:Patch-Clamp Techniques