title: Heritable targeted inactivation of myostatin gene in yellow catfish (Pelteobagrus fulvidraco) using engineered zinc finger nucleases.
abstract: Yellow catfish (Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in China. However, its small size and lower meat yield limit its edible value. Myostatin (MSTN) is a negative regulator of mammalian muscle growth. But, the function of Mstn in fish remains elusive. To explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass, we performed targeted disruption of mstn in yellow catfish using engineered zinc-finger nucleases (ZFNs). Employing zebrafish embryos as a screening system to identify ZFN activity, we obtained one pair of ZFNs that can edit mstn in yellow catfish genome. Using the ZFNs, we successfully obtained two founders (Founder July29-7 and Founder July29-8) carrying mutated mstn gene in their germ cells. The mutated mstn allele inherited from Founder July29-7 was a null allele (mstn(nju6)) containing a 4 bp insertion, predicted to encode function null Mstn. The mutated mstn inherited from Founder July29-8 was a complex type of mutation (mstn(nju7)), predicted to encode a protein lacking two amino acids in the N-terminal secretory signal of Mstn. Totally, we obtained 6 mstn(nju6/+) and 14 mstn(nju7/+) yellow catfish. To our best knowledge, this is the first endogenous gene knockout in aquaculture fish. Our result will help in understanding the roles of mstn gene in fish.
authors.affiliation: Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Study, Nanjing University, Nanjing, China.
authors.forename: Zhangji
authors.initials: Z
authors.lastname: Dong
authors.forename: Jiachun
authors.initials: J
authors.lastname: Ge
authors.forename: Kui
authors.initials: K
authors.lastname: Li
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xu
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
authors.forename: Junbo
authors.initials: J
authors.lastname: Li
authors.forename: Wenshuang
authors.initials: W
authors.lastname: Jia
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Dong
authors.forename: Shasha
authors.initials: S
authors.lastname: Cao
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.forename: Jianlin
authors.initials: J
authors.lastname: Pan
authors.forename: Qingshun
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D020868:Gene Silencing; D018390:Gene Targeting; D005818:Genetic Engineering; D040582:Inheritance Patterns / Q000235:genetics*; D008969:Molecular Sequence Data; D055435:Myostatin / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D055435:Myostatin; D004720:Endonucleases