title: Pseudomonas zeshuii sp. nov., isolated from herbicide-contaminated soil.
abstract: A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1(T), was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, PR China. Strain BY-1(T) grew optimally at pH 7.0 and 30-35 °C in the presence of 0.5% (w/v) NaCl. Analysis of 16S rRNA gene sequences revealed that strain BY-1(T) fell within the radiation of the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas luteola IAM 13000(T) (99.5%) and Pseudomonas duriflava HR2(T) (97.3%); the levels of sequence similarity with respect to other recognized species of the genus Pseudomonas were <96.7%. Strain BY-1(T) showed low DNA-DNA relatedness values with Pseudomonas luteola IAM 13000(T) (29 ± 3.1%) and Pseudomonas duriflava HR2(T) (21 ± 1.5 %). The G+C content of the genomic DNA of strain BY-1(T) was 55.3 mol%. The major fatty acids were C(18:1), C(16:0) and summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major ubiquinone was Q-9. Data obtained in this study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas zeshuii sp. nov. is proposed. The type strain is BY-1(T) (=KACC 15471(T)=ACCC 05688(T)).
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhaozhong
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.|Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Minggen
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006540:Herbicides; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006540:Herbicides; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014451:Ubiquinone