title: Tea catechin epigallocatechin gallate inhibits Streptococcus mutans biofilm formation by suppressing gtf genes.
abstract: OBJECTIVE
  The anti-cariogenic properties of tea have been suggested for decades. Tea polyphenols, especially epigallocatechin gallate (EGCG), have been shown to inhibit dental plaque accumulation, but the exact mechanisms are not clear at present. We hypothesise that EGCG suppresses gtf genes in Streptococcus mutans at the transcriptional level disrupting the initial attachment of S. mutans and thus the formation of mature biofilms.
  
  
  METHODS
  In this study, the effect of EGCG on the sucrose-dependent initial attachment of S. mutans UA159 in a chemically defined medium was monitored over 4 h using a chamber slide model. The effects of EGCG on the aggregation and gtf B, C, D gene expression of S. mutans UA159 were also examined.
  
  
  RESULTS
  It was found that EGCG (7.8-31.25 μg/ml) exhibited dose-dependent inhibition of the initial attachment of S. mutans UA159. EGCG did not induce cellular aggregation of S. mutans UA159 at concentrations less than 78.125 μg/ml. Analysis of data obtained from real-time PCR showed that EGCG at sub-MIC level (15.6 μg/ml) significantly suppressed the gtf B, C, D genes of S. mutans UA159 compared with the non-treated control (p < 0.05).
  
  
  CONCLUSIONS
  These findings suggest that EGCG may represent a novel, natural anti-plaque agent that inhibits the specific genes associated with bacterial biofilm formation without necessarily affecting the growth of oral bacteria.
authors.affiliation: Department of Pediatric Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Xue D
authors.initials: XD
authors.lastname: Zhou
authors.forename: Christine D
authors.initials: CD
authors.lastname: Wu
mesh_terms: D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013295:Streptococcus mutans / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013662:Tea / Q000737:chemistry*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D013662:Tea; D037521:Virulence Factors; D002392:Catechin; C045651:epigallocatechin gallate; D005964:Glucosyltransferases