title: Resveratrol reduces TNF-α-induced U373MG human glioma cell invasion through regulating NF-κB activation and uPA/uPAR expression.
abstract: BACKGROUND
  High invasiveness of glioma cells is one of the reasons that patients with malignant glioma have a poor prognosis. Resveratrol, a plant compound abundant in the peel of grapes, has been suggested as a potential cancer chemopreventive agent. Therefore, we investigated the effect of resveratrol on glioma cell invasion.
  
  
  METHODS
  The effect of resveratrol on U373MG human glioma cell invasion was assessed by Matrigel assay and methylthiazoltetrazolium assay. Western blotting and reverse transcription-polymerase chain reaction were performed to elucidate the action mechanism of resveratrol.
  
  
  RESULTS
  Resveratrol reduced tumor necrosis factor (TNF)-α-induced U373MG human glioma cell invasion. In addition, resveratrol repressed nuclear factor kappa B (NF-κB) activation and down-regulated mRNA expression of urokinase plasminogen activator (uPA) and its receptor in TNF-α-treated glioma cells.
  
  
  CONCLUSIONS
  These findings suggest that resveratrol could prevent glioma cell invasion via inhibiting proteolysis of extracellular matrix.
authors.affiliation: Department of Anatomy and Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, 92 Chilam-Dong, Jinju 660-751, Korea.
authors.forename: Jinhyun
authors.initials: J
authors.lastname: Ryu
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Ku
authors.forename: Yeon Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Jeong
authors.forename: Seokmin
authors.initials: S
authors.lastname: Kang
authors.forename: Jungil
authors.initials: J
authors.lastname: Choi
authors.forename: Yeongae
authors.initials: Y
authors.lastname: Yang
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Gu Seob
authors.initials: GS
authors.lastname: Roh
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Gyeong Jae
authors.initials: GJ
authors.lastname: Cho
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
authors.forename: Nayoung
authors.initials: N
authors.lastname: Kim
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Kang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000473:pathology; D006801:Humans; D007797:Laminin / Q000737:chemistry; D016328:NF-kappa B / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D007797:Laminin; D016328:NF-kappa B; C524898:PLAUR protein, human; D011509:Proteoglycans; D012333:RNA, Messenger; D055293:Receptors, Urokinase Plasminogen Activator; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C059485:matrigel; D003094:Collagen; D014568:Urokinase-Type Plasminogen Activator; D000077185:Resveratrol