title: Comparative proteomics analysis of serum proteins in ulcerative colitis patients.
abstract: In the present study, we investigated the differentially expressed proteins associated with ulcerative colitis (UC) using proteomic methods. Two-dimensional electrophoresis (2-DE) technology was performed to separate the total proteins of ulcerative tissues from those of the normal tissues of UC patients. PDQuest software was applied to analyze the obtained 2-DE images. Candidate protein spots between the two groups were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics analysis. The well resolution and reproducible 2-DE patterns of UC and normal tissues were established. Of the 12 differentially expressed proteins, 9 were successfully identified, of which 6 proteins were up-regulated including apolipoprotein C-III, haptoglobin, receptor tyrosine kinase, aldehyde reductase, pericentriolar material 1, and heat shock factor protein 2, and 3 were down-regulated including keratin, filamin A-interacting protein 1, and tropomyosin 3. These identified proteins were related to hormonal modulation, immune response, oxidative stress, and signal conduction. The 2-DE protein expression profile of the UC tissues displays an obvious difference from that of the normal controls. Various proteins may be involved in the occurrence of UC.
authors.affiliation: Department of Gastenerology, 309 Hospital, PLA, Beijing, 100091, China. nan01_li@yeah.net
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Xueming
authors.initials: X
authors.lastname: Wang
authors.forename: Yuefan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Junshan
authors.initials: J
authors.lastname: Zhai
authors.forename: Tuo
authors.initials: T
authors.lastname: Zhang
authors.forename: Kaihua
authors.initials: K
authors.lastname: Wei
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D002648:Child; D003093:Colitis, Ulcerative / Q000097:blood* / Q000378:metabolism* / Q000473:pathology; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007633:Keratins / Q000378:metabolism; D013058:Mass Spectrometry; D008875:Middle Aged; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D007633:Keratins