title: Immunophenotyping of hematopoietic progenitor cells: Comparison between cord blood and adult mobilized blood grafts.
abstract: OBJECTIVE
  To study the immunophenotype of hematopoietic progenitor cells from cord blood (CB) grafts (n = 39) in comparison with adult apheresis grafts (AG, n = 229) and pre-apheresis peripheral blood (PAPB) samples (n = 908) using flow cytometry analysis.
  
  
  METHODS
  First, we performed a qualitative analysis of CD34+ cell sub-populations in both CB and PAPB grafts using the standardized ISHAGE protocol and a wide panel of 20 monoclonal antibodies. Next, we studied some parameters, such as the age of mothers and the weight of newborns, which can influence the quality and the quantity of CD34+ cells from CB.
  
  
  RESULTS
  We found that the percentage of apoptotic cells was high in CB in comparison to PAPB (PAPB: 4.6% ± 2.6% vs CB: 53.4% ± 5.2%, P < 0.001). In CB, the weight of newborn and the age of the mother have the influence on CD34+ cells. The follow-up of Ag CD133 in the ISHAGE double platform protocol in association with CD45, CD34 and the 7'AAD shows an equal rate between the two cell populations CD133+CD45+CD34+ high and CD34+CD45+ high with a higher percentage. So, is the inclusion of Ac CD133 necessary in the present panel included in the ISHAGE method? Last part, we showed a significant presence of interferon γ in CB in comparison to PAPB, the annexin showing the high number of apoptotic cells in CB.
  
  
  CONCLUSIONS
  This study demonstrates that many different obstetric factors must be taken into account when processing and cryo-banking umbilical CB units for transplantation.
authors.affiliation: Nesrine Ben Azouna, Faouzi Jenhani, Immunology Research Unit, Faculty of Pharmacy of Monastir, Monsatir University, TU-5000 Monastir, Tunisia.
authors.forename: Nesrine Ben
authors.initials: NB
authors.lastname: Azouna
authors.forename: Lamia
authors.initials: L
authors.lastname: Berraeis
authors.forename: Zohra
authors.initials: Z
authors.lastname: Regaya
authors.forename: Faouzi
authors.initials: F
authors.lastname: Jenhani