title: A novel approach to the use of doxycycline-loaded biodegradable membrane and EDTA root surface etching in chronic periodontitis: a randomized clinical trial.
abstract: BACKGROUND
  The release profile of 25% doxycycline (DOX) gel loaded on a biodegradable collagen membrane (COL) after 24% EDTA root surface etching was evaluated.
  
  
  METHODS
  Thirty systemically healthy patients, each with at least one pair of contralateral interproximal intrabony defects ≥4 mm deep, along with an interproximal probing depth ≥6 mm and clinical attachment loss ≥4 mm, were randomized into two groups. Group 1 consisted of sites treated with open-flap debridement followed by placement of DOX gel-loaded COL (DOX-COL), whereas group 2 sites were treated with flap surgery followed by the placement of DOX-COL after EDTA etching of the exposed root surfaces (DOX-COL + EDTA). Samples of gingival crevicular fluid were obtained 1, 3, 7, 14, and 21 days after surgery. Separation was performed, and quantitative measurements of DOX were taken with a high-performance liquid chromatography. Clinical evaluation and follow-up for 6 months were performed.
  
  
  RESULTS
  At 21 days, DOX-COL + EDTA group showed 5.3 μg/mL value. However, no DOX was detected in samples of the DOX-COL group. DOX-COL + EDTA-treated group retained more DOX during the periods of 3, 7, 10, and 14 days than did the DOX-COL group.
  
  
  CONCLUSIONS
  EDTA root surface etching could enhance DOX availability in the gingival crevicular fluid after its release from the collagen membrane.
authors.affiliation: Department of Periodontology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt. hgamal1@hotmail.com
authors.forename: Ahmed Y
authors.initials: AY
authors.lastname: Gamal
authors.forename: Radi M
authors.initials: RM
authors.lastname: Kumper
mesh_terms: D020341:Absorbable Implants; D000134:Acid Etching, Dental / Q000379:methods*; D000328:Adult; D016301:Alveolar Bone Loss / Q000628:therapy; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000032:analysis; D002614:Chelating Agents / Q000627:therapeutic use*; D002851:Chromatography, High Pressure Liquid; D055113:Chronic Periodontitis / Q000628:therapy*; D003094:Collagen / Q000737:chemistry; D003692:Delayed-Action Preparations; D012534:Dental Scaling / Q000379:methods; D004058:Diffusion; D004318:Doxycycline / Q000008:administration & dosage* / Q000032:analysis; D004492:Edetic Acid / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D005782:Gels; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D010514:Periodontal Pocket / Q000628:therapy; D016745:Root Planing / Q000379:methods; D013524:Surgical Flaps; D014092:Tooth Root / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D003692:Delayed-Action Preparations; D005782:Gels; D008567:Membranes, Artificial; D003094:Collagen; D004492:Edetic Acid; D004318:Doxycycline