title: Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis.
abstract: A recombinant enzyme from Lysinibacillus fusiformis was expressed, purified, and identified as an oleate hydratase because the hydration activity of the enzyme was the highest for oleic acid (with a k                         (cat) of 850 min(-1) and a K                         (m) of 540 μM), followed by palmitoleic acid, γ-linolenic acid, linoleic acid, myristoleic acid, and α-linolenic acid. The optimal reaction conditions for the enzymatic production of 10-hydroxystearic acid were pH 6.5, 35 °C, 4% (v/v) ethanol, 2,500 U ml(-1) (8.3 mg ml(-1)) of enzyme, and 40 g l(-1) oleic acid. Under these conditions, 40 g l(-1) (142 mM) oleic acid was converted into 40 g l(-1) (133 mM) 10-hydroxystearic acid for 150 min, with a molar yield of 94% and a productivity of 16 g l(-1) h(-1), and olive oil hydrolyzate containing 40 g l(-1) oleic acid was converted into 40 g l(-1) 10-hydroxystearic acid for 300 min, with a productivity of 8 g l(-1) h(-1).
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Bi-Na
authors.initials: BN
authors.lastname: Kim
authors.forename: Young-Chul
authors.initials: YC
authors.lastname: Joo
authors.forename: Yeong-Su
authors.initials: YS
authors.lastname: Kim
authors.forename: Kyoung-Rok
authors.initials: KR
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001406:Bacillaceae / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D019301:Oleic Acid / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D000069463:Olive Oil; D010938:Plant Oils; D013229:Stearic Acids; D019301:Oleic Acid; C070376:10-hydroxystearic acid; D006836:Hydro-Lyases