title: Simple fabrication of polymer-based Trametes versicolor laccase for decolorization of malachite green.
abstract: A highly efficient and stable biocatalyst (denoted D201_Lac) was fabricated by encapsulating Trametes versicolor laccase within a macroporous and strongly basic exchange resin D201 through a simple adsorption process. Transmission electron micrographs and Fourier transform infrared spectra of the resultant D201_Lac proved that nanosized laccase clusters were embedded into the inner nano-pores/channels of D201. As compared to the free laccase, D201_Lac showed enhanced resistance in the pH range of 3-7 or at temperature of 30-60°C. Besides, negligible laccase was leached out from the host polymer D201 in solution of pH 3-7 and NaCl concentration up to 0.5M, which might be attributed to the electrostatic attraction and the possible twining between long-chain laccase and the cross-linking host resin. Continuous seven-cycle batch decoloration of malachite green demonstrates that decoloration efficiency of D201_Lac kept constant for more than 320-h operation.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Bingcai
authors.initials: B
authors.lastname: Pan
authors.forename: Ming
authors.initials: M
authors.lastname: Hua
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
mesh_terms: D001673:Biodegradation, Environmental / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D003116:Color; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007475:Ion Exchange Resins / Q000494:pharmacology*; D042845:Laccase / Q000378:metabolism*; D009994:Osmolar Concentration; D011108:Polymers / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D012394:Rosaniline Dyes / Q000302:isolation & purification*; D012996:Solutions; D013696:Temperature; D055454:Trametes / Q000187:drug effects / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D007475:Ion Exchange Resins; D011108:Polymers; D012394:Rosaniline Dyes; D012996:Solutions; C005095:malachite green; D042845:Laccase