title: Identification and characterization of novel immunomodulatory bursal-derived pentapeptide-II (BPP-II).
abstract: The bursa of Fabricius, the acknowledged central humoral immune organ, plays a vital role in B lymphocyte differentiation. However, there are few reports of the molecular basis of the mechanism on immune induction and potential antitumor activity of bursal-derived peptides. In this paper, a novel bursal-derived pentapeptide-II (BPP-II, MTLTG) was isolated and exerted immunomodulatory functions on antibody responses in vitro. Gene microarray analyses demonstrated that BPP-II regulated expression of 2478 genes in a mouse-derived hybridoma cell line. Immune-related gene ontology functional procedures were employed for further functional analysis. Furthermore, the majority of BPP-II-regulated pathways were associated with immune responses and tumor processes. Moreover, BPP-II exhibited immunomodulatory effects on antigen-specific immune responses in vivo, including enhancement of avian influenza virus (H9N2 subtype)-specific antibody and cytokine production and modification of T cell immunophenotypes and lymphocyte proliferation. Finally, BPP-II triggered p53 expression and stabilization and selectively inhibited tumor cell proliferation. These data identified the multifunctional factor, BPP-II, as a novel biomaterial representing an important linking between the humoral central immune system and immune induction, including antitumor. Information generated in this study elucidates further the mechanisms involved in humoral immune system and represents the potential basis of effective immunotherapeutic strategies for treating human tumors and immune improvement.
authors.affiliation: Division of Key Laboratory of Animal Disease Diagnosis and Immunology, Department of Agriculture of China, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Feng
authors.forename: Qing-Tao
authors.initials: QT
authors.lastname: Liu
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ma
authors.forename: Wen-Lei
authors.initials: WL
authors.lastname: Deng
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Wei
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Qiu
authors.forename: Fang-Quan
authors.initials: FQ
authors.lastname: Wang
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Gu
authors.forename: Feng-Juan
authors.initials: FJ
authors.lastname: Wang
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Zheng
authors.forename: Hassan
authors.initials: H
authors.lastname: Ishag
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002060:Bursa of Fabricius / Q000737:chemistry / Q000276:immunology; D045744:Cell Line, Tumor; D002645:Chickens / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D053127:Influenza A Virus, H9N2 Subtype / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007155:Immunologic Factors; D009842:Oligopeptides; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53