title: Proteomic profile evolution during steatosis development in ducks.
abstract: We investigated a protein profile evolution during steatosis in ducks using 2-dimensional electrophoresis gels to better understand the mechanisms underlying liver steatosis at the level of hepatic proteins in waterfowl. Two-dimensional electrophoresis gels were performed in the liver at different stages of steatosis in the duck. Mule ducks were slaughtered after 0, 14, or 23 meals of overfeeding, according to commercial conditions. Thirty-one proteic spots were differentially expressed between 3 or 2 durations of overfeeding: 3 spots were differentially expressed between the 3 times and 28 spots were differentially expressed between 2 times. The identified proteins (14) could be regrouped into 5 categories: enzymes, translation factors, proteins involved in cell structure, proteins with antioxidant properties, and proteins that can link calcium. This study opens new research areas in the understanding of steatosis in waterfowl, such as cell structure and oxidative stress.
authors.affiliation: Université de Toulouse, Institut National Polytechnique de Toulouse - Ecole Nationale Supérieure Agronomique de Toulouse, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Bax
authors.forename: C
authors.initials: C
authors.lastname: Chambon
authors.forename: N
authors.initials: N
authors.lastname: Marty-Gasset
authors.forename: H
authors.initials: H
authors.lastname: Remignon
authors.forename: X
authors.initials: X
authors.lastname: Fernandez
authors.forename: C
authors.initials: C
authors.lastname: Molette
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D030161:Avian Proteins / Q000032:analysis / Q000378:metabolism; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism; D002853:Chromatography, Liquid / Q000662:veterinary; D004372:Ducks; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005234:Fatty Liver / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*; D020869:Gene Expression Profiling / Q000662:veterinary; D008099:Liver / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011201:Poultry Diseases / Q000201:enzymology / Q000378:metabolism*; D014176:Protein Biosynthesis; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000662:veterinary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary
chemical_list: D000975:Antioxidants; D030161:Avian Proteins; D002352:Carrier Proteins; D020543:Proteome; D002118:Calcium