title: Low-cost three-dimensional imaging system combining fluorescence and ultrasound.
abstract: In this paper, we present a dual-modality imaging system combining three-dimensional (3D) continuous-wave transillumination fluorescence tomography with 3D ultrasound (US) imaging. We validated the system with two phantoms, one containing fluorescent inclusions (Cy5.5) at different depths, and another varying-thickness semicylindrical phantom. Using raster scanning, the combined fluorescence/US system was used to collect the boundary fluorescent emission in the X-Y plane, as well as recovered the 3D surface and position of the inclusions from US signals. US images were segmented to provide soft priors for the fluorescence image reconstruction. Phantom results demonstrated that with priors derived from the US images, the fluorescent reconstruction quality was significantly improved. As further evaluation, we show pilot in vivo results using an Apo-E mouse to assess the feasibility and performance of this system in animal studies. Limitations and potential to be used in artherosclerosis studies are then discussed.
authors.affiliation: École Polytechnique de Montréal, Institute of Biomedical Engineering, Montreal, H3C 3A7, Canada.
authors.forename: Baoqiang
authors.initials: B
authors.lastname: Li
authors.forename: Maxime
authors.initials: M
authors.lastname: Abran
authors.forename: Carl
authors.initials: C
authors.lastname: Matteau-Pelletier
authors.forename: Léonie
authors.initials: L
authors.lastname: Rouleau
authors.forename: Tina
authors.initials: T
authors.lastname: Lam
authors.forename: Rishi
authors.initials: R
authors.lastname: Sharma
authors.forename: Eric
authors.initials: E
authors.lastname: Rhéaume
authors.forename: Ashok
authors.initials: A
authors.lastname: Kakkar
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Tardif
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lesage
mesh_terms: D000818:Animals; D006321:Heart / Q000033:anatomy & histology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014054:Tomography / Q000379:methods*; D014463:Ultrasonography / Q000379:methods*