title: Ultrasmall gold nanoparticles for highly specific isolation/enrichment of N-linked glycosylated peptides.
abstract: In recent years, gold nanoparticles have been increasingly utilized as a promising material for biomedical analysis. We report here for the first time the synthesis of ultrasmall gold nanoparticles with core diameter of 1.2 nm functionalized with hydrazide groups and their use in isolation/enrichment of N-glycosylated peptides. Hydrazide-functionalized gold nanoparticles showed excellent stability in biological samples and exhibited a large capacity for peptide capturing. The captured peptides from tested standard glycoproteins were found to be highly specific as determined by Agilent HPLC chip and quadrupole time-of-flight (Q-TOF) mass spectrometer. The hydrazide-functionalized gold nanoparticles were successfully utilized in the isolation of a real proteome complex, which showed that more than 90% of captured product was glycopeptide. These results demonstrate that the ultrasmall gold nanoparticles can be used for a high-throughput analysis platform of glycoproteins.
authors.affiliation: Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, 7-45 Songdo-dong, Yeonsu-ku, Incheon 406-840, South Korea.
authors.forename: Trang Huyen
authors.initials: TH
authors.lastname: Tran
authors.forename: SunYoung
authors.initials: S
authors.lastname: Park
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Lee
authors.forename: Sungsuk
authors.initials: S
authors.lastname: Park
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Ok-Hee
authors.initials: OH
authors.lastname: Kim
authors.forename: Byung-chul
authors.initials: BC
authors.lastname: Oh
authors.forename: Dongil
authors.initials: D
authors.lastname: Lee
authors.forename: Hookeun
authors.initials: H
authors.lastname: Lee
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006020:Glycopeptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D020543:Proteome / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D020543:Proteome; D006046:Gold