title: MicroRNAs miR-27a and miR-143 regulate porcine adipocyte lipid metabolism.
abstract: MicroRNAs (miRNAs) are non-coding small RNAs that play roles in regulating gene expression. Some miRNAs have been classed as epigenetic regulators of metabolism and energy homeostasis. Previous reports indicated that the miRNAs miR-27a and miR-143 were involved in lipid metabolism in human and rodents. To determine the roles of porcine miR-27a and miR-143 in adipocyte lipid metabolism, porcine adipocytes were cultured and allowed to induce differentiation for 10 days. The lipid-filled adipocytes were then transfected with miRNA mimics and inhibitors. We measured how the indicators of adipogenesis and adipolysis in porcine adipocytes were affected by the over-expression and by the inhibition of both miR-27a and miR-143. The results indicated that the over-expression of miR-27a could accelerate adipolysis releasing significantly more glycerol and free fatty acids than the negative control (P < 0.001), while the mimic of miR-143 expression, promoted adipogenesis by accumulating more triglycerides (P < 0.001) in the adipocytes. In addition, we demonstrated that there was good correlation (r > 0.98, P < 0.001) between the indicators of adipolysis in cell lysates and in the culture medium.
authors.affiliation: Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, China; E-Mails:  wanttao@gmail.com  (T.W.);  mingzhou.li@163.com  (M.L.);  79184122@qq.com  (J.G.);  45085733@qq.com  (P.L.);  253620495@qq.com  (H.W.);  610810793@qq.com  (Y.G.).
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.forename: Jiuqiang
authors.initials: J
authors.lastname: Guan
authors.forename: Penghao
authors.initials: P
authors.lastname: Li
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wang
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Guo
authors.forename: Surong
authors.initials: S
authors.lastname: Shuai
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D014162:Transfection; D014280:Triglycerides / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D014280:Triglycerides