title: Regulation of soluble VEGFR-2 secreted by microvascular endothelial cells derived from human BPH.
abstract: BACKGROUND
  Recently, it was reported that the soluble vascular endothelial growth factor receptor-2 (sVEGFR-2) is secreted by microvascular endothelial cells from human BPH (HPECs). The purpose of this study was to investigate the modulation of sVEGFR-2 by common endothelial cell stimulators. In addition, the physiological role of sVEGFR-2 with regard to the VEGF-stimulated proliferation of HPEC was investigated.
  
  
  METHODS
  HPECs were isolated and cultured from fresh BPH tissue. After the incubation of HPECs either with adenosine triphosphate (ATP), interleukin (IL)-6, IL-8 or IL-12, the secretion of sVEGFR-2 was measured by enzyme-linked immunosorbent assay. For measurement of HPEC proliferation influenced by sVEGFR-2, VEGF-stimulated HPEC was cultured with/without sVEGFR-2. Cell proliferation was assessed with the Alamar Blue method.
  
  
  RESULTS
  The sVEGFR-2 secretion was increased by ATP and decreased by IL-12 and IL-8, respectively. IL-6 did not show any significant effect on sVEGFR-2 secretion of HPECs. HPEC proliferation was significantly inhibited by sVEGFR-2.
  
  
  CONCLUSIONS
  In this study, our data suggest that the secretion of sVEGFR-2 by microvascular endothelial cells from prostate origin is influenced by multiple endothelial cell stimulators. Furthermore, our data suggest that sVEGFR-2 acts as an antiangiogenic factor.
authors.affiliation: Institute of Clinical Chemistry, Transfusion, and Laboratory Medicine, University Clinic BG Bergmannsheil, BG-University Hospital Bergmannsheil, Ruhr-University of Bochum, Bochum, Germany. Assem.Aweimer@rub.de
authors.forename: A
authors.initials: A
authors.lastname: Aweimer
authors.forename: T
authors.initials: T
authors.lastname: Stachon
authors.forename: A
authors.initials: A
authors.lastname: Tannapfel
authors.forename: M
authors.initials: M
authors.lastname: Köller
authors.forename: M C
authors.initials: MC
authors.lastname: Truss
authors.forename: A
authors.initials: A
authors.lastname: Stachon
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D008297:Male; D011467:Prostate / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D018664:Interleukin-12; D000255:Adenosine Triphosphate; D040301:Vascular Endothelial Growth Factor Receptor-2