title: Complete genomic sequence analysis reveals a novel fabavirus infecting cucurbits in China.
abstract: The complete genome sequence of a cucurbit-infecting fabavirus was determined. Sequence analysis revealed that it had a genomic organization typical of fabaviruses, with genome segment sizes of 5870 nt (RNA-1) and 3294 nt (RNA-2). It shared CP and Pro-Pol amino acid sequence identities of 52.0-58.9% with those of reported fabaviruses. ELISA and western blots gave no cross-reactions between this cucurbit virus and broad bean wilt viruses 1 and 2. Based on molecular and serological criteria for species demarcation in the genus Fabavirus, the virus represents a distinct species, for which the species name Cucurbit mild mosaic virus (CuMMV) is proposed.
authors.affiliation: State Key Laboratory for Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, P. R. China. dongsw05@gmail.com
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Dong
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Xiang
authors.forename: Qiao-Xia
authors.initials: QX
authors.lastname: Shang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Yu
authors.forename: Cheng-Gui
authors.initials: CG
authors.lastname: Han
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D002681:China; D016000:Cluster Analysis; D003429:Cross Reactions; D028464:Cucurbita / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D019181:Fabavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral