title: Serum PON-1 activity but not Q192R polymorphism is related to the extent of atherosclerosis.
abstract: OBJECTIVE
  Paraoxonase-1 (PON1) is an antioxidant enzyme located in high density lipoprotein (HDL). PON1 was defined as a protective factor against atherosclerosis. The aim of this study was to investigate the possible relationship between serum paraoxonase (PONase), homocysteine thiolactonase (HTase) activities and PON1 Q192R polymorphism, and the extent and severity of atherosclerosis.
  
  
  METHODS
  Blood specimens were collected from 142 individuals who had no coronary artery lesions angiographically (control group) and 128 individuals who had angiographically documented coronary artery disease of several degrees (patient group). The extent and severity of arterial lesions were evaluated by the Gensini scoring system. PONase and HTase activities were measured in serum using a spectrophotometric method. PON1 Q192R polymorphism was evaluated using PCR-RFLP after DNA isolation from blood.
  
  
  RESULTS
  Serum PONase and HTase activities were significantly lower in the patient group than in healthy controls (135.7±56.0U/mL vs 153.8±62.0U/mL, p< 0.05; 36.0±6.1 U/mL vs 43.0±4.04 U/mL, p< 0.01; respectively). In the patient group, there was a negative correlation between PONase, HTase activities and the Gensini score (r=-0.168, p= 0.039; r=-0.164, p= 0.006, respectively). In both groups, there was no significant difference in the distribution of PON1 Q192R polymorphism. In the patient group, the distribution of Gensini scores according to genotypes was not significant.
  
  
  CONCLUSIONS
  It has been concluded that serum PONase and HTase activities might be a more relevant marker than PON1 genotype in evaluating the extent and severity of atherosclerosis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey. ahmetb@hacettepe.edu.tr
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bayrak
authors.forename: Tülin
authors.initials: T
authors.lastname: Bayrak
authors.forename: S Lale
authors.initials: SL
authors.lastname: Tokgözoglu
authors.forename: Bilge
authors.initials: B
authors.lastname: Volkan-Salanci
authors.forename: Ali
authors.initials: A
authors.lastname: Deniz
authors.forename: Bünyamin
authors.initials: B
authors.lastname: Yavuz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Alikasifoglu
authors.forename: Ediz
authors.initials: E
authors.lastname: Demirpençe
mesh_terms: D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood* / Q000235:genetics*; D050197:Atherosclerosis / Q000175:diagnosis* / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human