title: Hypertonic stimulation inhibits synaptic transmission in hippocampal slices through decreasing pre-synaptic voltage-gated calcium current.
abstract: Acute changes in the cerebrospinal fluid osmotic pressure modulate the brain excitability. The present study investigated the effect of hypertonic stimulation on the synaptic transmission in hippocampal slices. It was found that the slope of excitatory postsynaptic potential (EPSP) in hippocampal CA1 area was inhibited after the hypertonic treatment. Accompanied with the inhibition in EPSP slope, the paired-pulse facilitation (PPF) was increased by hypertonicity. Transient receptor potential vanilloid 4 (TRPV4 receptor) antagonists did not block hypertonicity-action. High voltage-gated calcium current (I(HVA)) in hippocampal CA3 neurons was decreased by hypertonicity, whereas the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-induced current in hippocampal CA1 neurons was unaffected. Additionally, inhibition of phosphatidylinositol 3-kinase (PI3K) or protein kinase A (PKA) markedly attenuated hypertonicity-induced decrease of I(HVA), whereas antagonism of phosphorylated ERK1/2 mitogen-activated protein kinase (pERK1/2) had no effect. We conclude that hypertonic stimulation inhibits synaptic transmission in hippocampal slices through decreasing pre-synaptic voltage-gated calcium current.
authors.affiliation: Department of Physiology, Nanjing Medical University, Nanjing 210019, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Changjin
authors.initials: C
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000502:physiology*; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D006624:Hippocampus / Q000502:physiology*; D006982:Hypertonic Solutions; D008297:Male; D009924:Organ Culture Techniques; D009997:Osmotic Pressure; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D015220:Calcium Channels; D006982:Hypertonic Solutions