title: Nitric oxide restrain root growth by DNA damage induced cell cycle arrest in Arabidopsis thaliana.
abstract: Nitric oxide (NO) participates in the regulation of diverse functions in plant cells. However, different NO concentrations may trigger different pathways during the plant development. At basal levels of NO, plants utilize the NO signaling transduction pathway to facilitate plant growth and development, whereas higher concentrations trigger programmed cell death (PCD). Our results show that NO lower than the levels causing PCD, but higher than the basal levels induce DNA damage in root cells in Arabidopsis as witnessed by a reduction in root growth, rather than cell death, since cells retain the capacity to differentiate root hairs. The decrease in meristematic cells and increase in DNA damage signals in roots in responses to NO are in a dose dependent manner. The restraint of root growth is due to cell cycle arrest at G1 phase which is caused by NO induced DNA damage, besides a second arrest at G2/M existed in NO supersensitive mutant cue1. The results indicate that NO restrain root growth via DNA damage induced cell cycle arrest.
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Sciences, Capital Normal University, Beijing, PR China. sulanb@sina.com
authors.forename: Sulan
authors.initials: S
authors.lastname: Bai
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Yao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yaochuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lihong
authors.initials: L
authors.lastname: Xiao
authors.forename: Jinzheng
authors.initials: J
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Weizhong
authors.initials: W
authors.lastname: Liu
authors.forename: Yikun
authors.initials: Y
authors.lastname: He
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D016923:Cell Death / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000235:genetics; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide