title: A novel TCTP gene from the crustacean Eriocheir sinensis: possible role involving metallic Cu2+ stress.
abstract: The translationally controlled tumor protein (TCTP), which was initially described as a growth-related protein, primarily expresses proteins for numerous biological processes in eukaryotes. In the present study, the TCTP gene in the Chinese mitten crab Eriocheir sinensis (Es-TCTP) was identified from tissues of the hepatopancreas, by the construction of a cDNA library and the rapid amplification of cDNA ends. The results showed that the full length of Es-TCTP cDNA comprises 727 nucleotides, with an open reading frame of 507 bp that encodes 168 amino acid residues. The deduced amino acid sequence of Es-TCTP has a 51%-92% similarity to TCTPs of other eukaryotic species. The mRNA transcripts of Es-TCTP were detected by using RT-PCR for all tissues, with the highest level occurring in the hepatopancreas. In addition, temporal expression of the Es-TCTP was measured to analyze the role of Es-TCTP in an acute stress condition after CuSO(4) treatment. The expression of the Es-TCTP transcripts in hepatopancreas had no significant difference at the first 4 h, but increased significantly after 8 h, peaked at 24 h (4.3-fold higher than the control), and was still high after 72 h. Our results indicate that Es-TCTP is an acute-phase protein that is involved in Cu(2+) stress, with a possible anti-stress function in invertebrates.
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China. qun_300@hotmail.com
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Di-An
authors.initials: DA
authors.lastname: Fang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003386:Brachyura / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D019327:Copper Sulfate / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D043143:Hepatopancreas / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013312:Stress, Physiological; D013997:Time Factors; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D012333:RNA, Messenger; D000090324:Tumor Protein, Translationally-Controlled 1; D019327:Copper Sulfate