title: Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer.
abstract: ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone-DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Shahian
authors.forename: Geeta J
authors.initials: GJ
authors.lastname: Narlikar
mesh_terms: D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling; D013223:Statistics as Topic
chemical_list: D005456:Fluorescent Dyes; D006657:Histones; D009707:Nucleosomes; D004247:DNA