title: Paraformaldehyde fixation of Drosophila testes.
abstract: This protocol describes paraformaldehyde fixation of Drosophila testes. The procedure preserves F-actin-containing structures because it does not involve the use of methanol. Thus, it is particularly suitable for contractile ring and fusome visualization. However, it does not preserve cell morphology and microtubules as efficiently as methanol-acetone fixation and does not permit clear detection of the Y loops.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bonaccorsi
authors.forename: Maria G
authors.initials: MG
authors.lastname: Giansanti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenci
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
mesh_terms: D000818:Animals; D004330:Drosophila / Q000033:anatomy & histology*; D005404:Fixatives / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism*; D008297:Male; D011108:Polymers / Q000378:metabolism*; D013737:Testis / Q000033:anatomy & histology; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D005404:Fixatives; D011108:Polymers; D005557:Formaldehyde; C003043:paraform