title: The Arabidopsis JAZ2 promoter contains a G-Box and thymidine-rich module that are necessary and sufficient for jasmonate-dependent activation by MYC transcription factors and repression by JAZ proteins.
abstract: Jasmonate (JA) is a critical hormone for both plant defense and reproductive development. Until now, early JA-responsive promoters have not been well characterized. To identify the cis-acting DNA element involved in the early JA response at the transcriptional level, we analyzed the promoter of the Arabidopsis gene encoding jasmonate-ZIM domain2 protein (JAZ2). The full-length JAZ2 promoter in JAZ2::GUS plants is active in vegetative and reproductive tissue, with expression in stamen filaments being dependent on JA biosynthesis. We identified a G-box element in the JAZ2 promoter that is required for JA-mediated promoter activation in carrot protoplasts and Arabidopsis seedlings. Three copies of a G-box and flanking sequences has autonomous JA-dependent activity and was transactivated by MYC2, MYC3 and MYC4 transcription factors in carrot protoplasts. Expression of MYC2, MYC3 or MYC4 fused to an EAR (ETHYLENE RESPONSE FACTOR-associated amphiphilic repression) motif, or expression of JAZ1 or JAZ6 all repressed JA- and MYC-dependent activation of the JAZ2 promoter. A thymidine-rich sequence 3' to the G-box was required for full JA activation of the JAZ2 promoter. Because the G-box is also present in genes unresponsive to JA, we propose that this thymidine-rich sequence together with the G-box provides JA specificity to promoters induced early in the JA response. Together, these results indicate that an extended G-box located in the promoter region of an early JA-responsive gene is required for gene induction in vivo, probably through selective binding of MYC2, MYC3 and MYC4 transcription factors.
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
authors.forename: Pablo
authors.initials: P
authors.lastname: Figueroa
authors.forename: John
authors.initials: J
authors.lastname: Browse
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D018552:Daucus carota / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D003517:Cyclopentanes; C000602232:JAZ2 protein, Arabidopsis; C473611:MYC2 protein, Arabidopsis; C473612:MYC3 protein, Arabidopsis; C473613:MYC4 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D012097:Repressor Proteins; D015534:Trans-Activators; C011006:jasmonic acid; D013936:Thymidine