title: Administration of antisense DNA for GPR39-1b causes anxiolytic-like responses and appetite loss in rats.
abstract: The G protein-coupled receptor 39-b (GPR39-1b) is a splice variant of which is expressed in the central nervous and gastrointestinal systems. Previously, GPR39-1b was proposed to be the receptor for obestatin, but current evidence does not support this hypothesis. The purpose of the present work was to identify the role of GPR39-1b in anxiety and eating behaviors. Antisense oligonucleotides were infused at a constant rate into the cerebral lateral ventricles of rats and their effect on anxiety-like behavior and food intake was monitored. GPR39-1b antisense oligonucleotides produced anxiolytic-like effects in the elevated-plus maze test and in the black and white box test. Antisense oligonucleotides also decreased food intake. These results indicate that inhibition of GPR39-1b induces a decrease in anxiety-related behaviors and disturbs appetite.
authors.affiliation: Department of Neuropsychiatry, Oita University Faculty of Medicine, Hasama-Machi, Yufu-Shi, Oita 879-5593, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ishitobi
authors.forename: Jotaro
authors.initials: J
authors.lastname: Akiyoshi
authors.forename: Shuhei
authors.initials: S
authors.lastname: Honda
authors.forename: Taiga
authors.initials: T
authors.lastname: Ninomiya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kanehisa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Jusen
authors.initials: J
authors.lastname: Tsuru
authors.forename: Koichi
authors.initials: K
authors.lastname: Isogawa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kitamura
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Fujikura
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics* / Q000378:metabolism; D001066:Appetite / Q000187:drug effects / Q000235:genetics; D001522:Behavior, Animal / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects / Q000235:genetics; D016373:DNA, Antisense / Q000008:administration & dosage*; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism; D004435:Eating / Q000187:drug effects* / Q000235:genetics; D004644:Emotions / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism
chemical_list: D016373:DNA, Antisense; C535178:GPR39 protein, rat; D043562:Receptors, G-Protein-Coupled