title: Integrating multiple microarray datasets on oral squamous cell carcinoma to reveal dysregulated networks.
abstract: BACKGROUND
  Oral squamous cell carcinoma (OSCC) is the sixth most common type of carcinoma worldwide. The pathogenic pathways involved in this cancer are mostly unknown; therefore, a better characterization of the OSCC gene expression profile would represent a considerable advance. The public availability of gene expression datasets was meant to obtain new insights on biological processes.
  
  
  METHODS
  We integrated 4 public microarray datasets on OSCC to evaluate the degree of consistency among the biological results obtained in these different studies and to identify common regulatory pathways that could be responsible for tumor growth.
  
  
  RESULTS
  Twelve altered cellular pathways implicated in OSCC and 4 genes altered in the extracellular matrix (ECM) receptor pathway were validated by quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  CONCLUSIONS
  Using 4 expression array datasets, we have developed a robust method for analyzing pathways altered in OSCC.
authors.affiliation: Anal-Colorectal Surgery Institute, No. 150 Central Hospital of PLA, Luoyang, China 471031.
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Liu
authors.forename: Yulong
authors.initials: Y
authors.lastname: Niu
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Chunfang
authors.initials: C
authors.lastname: Gao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D030541:Databases, Genetic; D030561:Databases, Nucleic Acid; D005109:Extracellular Matrix / Q000235:genetics / Q000473:pathology / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology*; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D016024:Receptors, Cytoadhesin / Q000502:physiology; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D016024:Receptors, Cytoadhesin