title: The first case of Legionella nagasakiensis isolation from hot spring water.
abstract: In August, 2010, strain HYMO-6 was isolated from a sample of hot spring water in Aomori, Japan. The 16S rDNA sequences (1,496bp) of this strain (accession number: AB597175) had a similarity of less than 96.6% to other Legionella species, prompting us to hypothesize that this strain might be a novel species belonging to the genus Legionella. However, in March of 2011, it was became clear that the HYMO-6 strain (=JCM 17450 =KCTC 23560 =DSM 24727) was Legionella nagasakiensis CDC-1796-JAP-E(T) (=ATCC BAA-1557(T) =JCM 15315(T)). When this strain was cultured on BCYEα agar at 36°C for 7 d, no long cells were observed. The dominant fatty acids of strain HYMO-6 were 16:1ω7c (32.4%), and the DNA G+C content was 42.0 mol%.
authors.affiliation: School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa 252-5201, Japan. furuhata@azabu-u.ac.jp
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furuhata
authors.forename: Akiko
authors.initials: A
authors.lastname: Edagawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Goto
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuyama
mesh_terms: D045482:Hot Springs / Q000382:microbiology*; D007875:Legionella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny