title: Isolation and characterization of  nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas.
abstract: Commercial  banana varieties are highly susceptible to fungal pathogens, as well as  bacterial pathogens, nematodes, viruses, and insect pests. The largest known  family of plant resistance genes encodes proteins with nucleotide-binding site  (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in  such genes in diverse plant species offer a means for the isolation of  candidate genes in banana that may be involved in plant defense. Six degenerate  PCR primers were designed to target NBS and additional domains were tested on  commercial banana species Musa acuminata subsp malaccensis and  the Musa AAB Group propagated in vitro and plants maintained in a  greenhouse. Total DNA was isolated by a modified CTAB extraction technique.  Four resistance gene analogs were amplified and deposited in GenBank and  assigned numbers HQ199833-HQ199836. The predicted amino acid sequences  compared to the amino acid sequences of known resistance genes (MRGL1, MRGL2, MRGL3, and MRGL4) revealed significant sequence similarity. The  presence of consensus domains, namely kinase-1a, kinase-2 and hydrophobic  domain, provided evidence that the cloned sequences belong to the typical  non-Toll/interleukin-1 receptor-like domain NBS-LRR gene family.
authors.affiliation: College of Natural Resources and Environment, South China Agriculture University, Wushan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: W H
authors.initials: WH
authors.lastname: Xu
authors.forename: Y X
authors.initials: YX
authors.lastname: Xie
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: J J
authors.initials: JJ
authors.lastname: Pu
authors.forename: Y X
authors.initials: YX
authors.lastname: Qi
authors.forename: H P
authors.initials: HP
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites / Q000235:genetics; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics* / Q000378:metabolism; D030541:Databases, Genetic; D004926:Escherichia coli; D007930:Leucine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D028521:Musa / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010802:Phylogeny; D057865:Plant Immunity / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
chemical_list: D002593:Cetrimonium Compounds; D018744:DNA, Plant; D009711:Nucleotides; D010940:Plant Proteins; D011994:Recombinant Proteins; D007930:Leucine; D000077286:Cetrimonium