title: Transcription factor Sp1 is involved in expressional regulation of coxsackie and adenovirus receptor in cancer cells.
abstract: Coxsackie and adenovirus receptor (CAR) was first known as a virus receptor. Recently, it is also known to have tumor suppressive activity such as inhibition of cell proliferation, migration, and invasion. It is important to understand how CAR expression can be regulated in cancers. Based on an existence of putative Sp1 binding site within CAR promoter, we investigated whether indeed Sp1 is involved in the regulation of CAR expression. We observed that deletion or mutation of Sp1 binding motif (-503/-498) prominently impaired the Sp1 binding affinity and activity of CAR promoter. Histone deacetylase inhibitor (TSA) treatment enhanced recruitment of Sp1 to the CAR promoter in ChIP assay. Meanwhile, Sp1 binding inhibitor suppressed the recruitment. Exogenous expression of wild-type Sp1 increased CAR expression in CAR-negative cells; meanwhile, dominant negative Sp1 decreased the CAR expression in CAR-positive cells. These results indicate that Sp1 is involved in regulation of CAR expression.
authors.affiliation: Division of Intractable Diseases, Center for Biomedical Sciences, National Institute of Health, Osong Health Technology Administration Complex, 187 Osongsaengmyeo2(i)-ro, Gangoemyeon, Cheongwon-gun, Chungcheongbuk-do 363-951, Republic of Korea.
authors.forename: Sun-Ku
authors.initials: SK
authors.lastname: Chung
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Joong-Yeon
authors.initials: JY
authors.lastname: Lim
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Park
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Hwang
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
authors.forename: Sang Ick
authors.initials: SI
authors.lastname: Park
mesh_terms: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D011991:Receptors, Virus / Q000096:biosynthesis*; D020218:Response Elements; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D056572:Histone Deacetylase Inhibitors; D011991:Receptors, Virus; D016329:Sp1 Transcription Factor; D025521:Tumor Suppressor Proteins