title: Non-cultured epidermal suspension in vitiligo: from laboratory to clinic.
abstract: BACKGROUND
  Medical treatments are ineffective in many patients and surgical methods have therefore been developed.
  
  
  OBJECTIVE
  A review of autologous non-cultured melanocyte grafting techniques is proposed to obtain a successful repigmentation of vitiligo macules.
  
  
  METHODS
  Initially in 1992, we had developed a simplified grafting method which was carried out in the following two steps: production of blisters on the depigmented lesions by freezing with liquid nitrogen and injection in each blister of a non-cultured suspension of epidermal cells. The cellular suspension was obtained from samples of skin of the hair scalp after trypsinization. This very simple technique could be used at the dermatologist's clinic. Since 1998 (Olsson MJ, Juhlin L), quite comparable but improved and more sophisticated techniques have been proposed for the surgical treatment of vitiligo. These techniques require a laboratory set up to perform the melanocyte transplantation. The donor zone was usually taken on the gluteal region. The time of trypsinization was reduced to 60 minutes at 37°C and the centrifuged cellular suspension added with hyaluronic acid (Van Geel) was directly applied on a dermabraded or laser abraded vitiligo lesions.
  
  
  RESULTS
  Whatever the technique chosen, repigmentation was evident within 25 to 30 days. Coalescence of the pigmented areas was spontaneously observed or obtained after UVB radiation. It is obvious that the complete repigmentation occurred more rapidly with the recent techniques compared with the initial method, but the efficiency was quite similar.
  
  
  CONCLUSIONS
  The use of non-cultured epidermal suspension appears to be an effective, safe, and simple method for treating patients with achromic areas lacking melanocytes.
authors.affiliation: Pigmentary Disorders Outpatient Clinic, Department of Dermatology, Saint-André Hospital, Bordeaux, France. yvongauthier@free.fr
authors.forename: Yvon
authors.initials: Y
authors.lastname: Gauthier
authors.forename: Laila
authors.initials: L
authors.lastname: Benzekri
mesh_terms: D004817:Epidermis; D006801:Humans; D008544:Melanocytes; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014820:Vitiligo