title: Development of an amperometric polyphenol biosensor based on fungal laccase immobilized on nitrocellulose membrane.
abstract: A method is described for construction of an amperometric polyphenol biosensor employing nitrocellulose membrane-bound laccase purified from cell-free extract of Ganoderma lucidum onto a Pt electrode. The biosensor showed optimum response within 10s, at 0.4 V in 0.1M acetate buffer, pH 6.0, and 35°C. Detection limit of the biosensor was 3.0 × 10(-8)M. Analytical recovery of added guaiacol was 97.00%. Within batch and between batch coefficients of variation were <0.97% and <1.26%, respectively. The sensor measured total phenolic content in fruit juices and alcoholic beverages. The enzyme electrode was used 100 times over 4 months, when stored at 4°C.
authors.affiliation: Department of Biochemistry, MD University, Rohtak, India. pundircs@rediffmail.com
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Pundir
authors.forename: Rachna
authors.initials: R
authors.lastname: Rawal
authors.forename: Sheetal
authors.initials: S
authors.lastname: Chawla
authors.lastname: Renuka
authors.forename: Ramesh Chandra
authors.initials: RC
authors.lastname: Kuhad
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D003101:Collodion / Q000737:chemistry*; D004058:Diffusion; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D016014:Linear Models; D055595:Mechanical Phenomena; D008567:Membranes, Artificial; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry; D016062:Porosity; D020940:Reishi / Q000201:enzymology*; D013499:Surface Properties; D013696:Temperature
chemical_list: D000975:Antioxidants; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D059808:Polyphenols; D003101:Collodion; D048271:Chitosan; D042845:Laccase; D005976:Glutaral