title: Reconstitution of modified chromatin templates for in vitro functional assays.
abstract: To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.
authors.affiliation: Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Miyong
authors.initials: M
authors.lastname: Yun
authors.forename: Chun
authors.initials: C
authors.lastname: Ruan
authors.forename: Jae-Wan
authors.initials: JW
authors.lastname: Huh
authors.forename: Bing
authors.initials: B
authors.lastname: Li
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005822:Genetic Vectors; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011851:Radioactivity; D011994:Recombinant Proteins / Q000378:metabolism; D013698:Templates, Genetic
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D011994:Recombinant Proteins; D004247:DNA; D006655:Histone Deacetylases; D008239:Lysine