title: Fractions of aqueous and methanolic extracts from tomato (Solanum lycopersicum L.) present platelet antiaggregant activity.
abstract: Cardiovascular disease (CVD) is the leading cause of death worldwide. Its prevention emphasizes three aspects: not smoking, physical activity and a healthy diet. Recently, we screened the antithrombotic activity of a selected group of fruits and vegetables. Among them, tomato showed an important effect. The aim of this study was to evaluate and characterize the platelet antiaggregatory activity of tomato (Solanum lycopersicum L.). For this, we obtained aqueous and methanolic tomato extracts and evaluated the effect of pH (2 and 10) and temperature (22, 60 and 100°C) on this activity. Furthermore, in order to isolate the antiaggregant principle, we separated tomato extracts into several fractions (A-D) by size exclusion chromatography. In addition, we evaluated the platelet antiaggregating activity ex vivo in Wistar rats. Aqueous and methanolic extracts of tomato treated at 22, 60 and 100°C and pH 2 and 10 still inhibited platelet aggregation (in vitro). Moreover, it was noted that one of the fractions (fraction C), from both aqueous and methanolic extracts, presented the highest activity (∼70% inhibition of platelet aggregation) and concentration dependently inhibited platelet aggregation significantly compared with control (P < 0.05). These fractions did not contain lycopene but presented two peaks of absorption, at 210 and 261 nm, compatible with the presence of nucleosides. In rats treated with tomato macerates, a mild platelet antiaggregating effect ex vivo was observed. Further studies are required to identify the molecules with platelet antiaggregating activity and antiplatelet mechanisms of action.
authors.affiliation: Departamento de Bioquímica Clínica e Inmunohematología, Programa de Investigación en Factores de Riesgo de Enfermedades Cardiovasculares (PIFRECV), Universidad de Talca.
authors.forename: Eduado J
authors.initials: EJ
authors.lastname: Fuentes
authors.forename: Luis A
authors.initials: LA
authors.lastname: Astudillo
authors.forename: Margarita I
authors.initials: MI
authors.lastname: Gutiérrez
authors.forename: Samuel O
authors.initials: SO
authors.lastname: Contreras
authors.forename: Luis O
authors.initials: LO
authors.lastname: Bustamante
authors.forename: Pia I
authors.initials: PI
authors.lastname: Rubio
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Moore-Carrasco
authors.forename: Marcelo A
authors.initials: MA
authors.lastname: Alarcón
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Fuentes
authors.forename: Daniel E
authors.initials: DE
authors.lastname: González
authors.forename: Iván F
authors.initials: IF
authors.lastname: Palomo
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000494:pharmacology; D002850:Chromatography, Gel; D004355:Drug Stability; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D002338:Carotenoids; D000077276:Lycopene