title: Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family.
abstract: We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high sensitivity, and ELISA with rBgP32 resulted in high specificity and in concordance with IFAT results.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Youn-Kyoung
authors.initials: YK
authors.lastname: Goo
authors.forename: Gabriel Oluga
authors.initials: GO
authors.lastname: Aboge
authors.forename: M Alaa
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Honglin
authors.initials: H
authors.lastname: Jia
authors.forename: Junya
authors.initials: J
authors.lastname: Yamagishi
authors.forename: Fujiko
authors.initials: F
authors.lastname: Sunaga
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Namikawa
authors.forename: Se-Yeoun
authors.initials: SY
authors.lastname: Cha
authors.forename: Hyung-Kwan
authors.initials: HK
authors.lastname: Jang
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D001403:Babesia / Q000276:immunology* / Q000378:metabolism; D001404:Babesiosis / Q000469:parasitology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D052940:Merozoites / Q000276:immunology / Q000378:metabolism; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins