title: Whole-cell biochips for online water monitoring.
abstract: Chip-integrated luminescent recombinant reporter bacteria were combined with fluidics and light detection systems to form a real-time water biomonitor. The biomonitor was exposed to a continuous water flow for up to ten days, in the course of which it was challenged with spikes of both model toxic compounds and toxic environmental samples. All simulated contamination events were reported within 0.5-2.5 h. Furthermore, the response pattern of the reporter bacteria was indicative of the nature of the contaminating chemicals. Efforts were aimed at improving signal quality and at the development of an alarm management software. Following further research, a device of the proposed design could be implemented in monitoring networks as an early warning system against water pollution by toxic chemicals.
authors.affiliation: The Alexander Silberman Institute of Life Sciences, Department of Plant and Environmental Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. tal.elad@mail.huji.ac.il
authors.forename: Tal
authors.initials: T
authors.lastname: Elad
authors.forename: Shimshon
authors.initials: S
authors.lastname: Belkin
mesh_terms: D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D017930:Genes, Reporter; D008163:Luminescent Measurements / Q000379:methods; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014877:Water Pollution, Chemical / Q000032:analysis
chemical_list: D014874:Water Pollutants, Chemical