title: The effects of resveratrol on  cyclooxygenase-1 and -2, nuclear factor kappa beta, matrix metalloproteinase-9, and sirtuin 1 mRNA  expression in hearts of streptozotocin-induced  diabetic rats.
abstract: Resveratrol  (RSV) has a beneficial role in the prevention of diabetes and alleviates some  diabetic complications, such as cardiomyopathy. We investigated  cyclooxygenase-1 (COX-1), COX-2, nuclear factor κB (NF-κB), matrix metalloproteinase-9 (MMP-9), and sirtuin 1  (SIRT1) mRNA expression levels in heart tissue after RSV treatment in  streptozotocin (STZ)-induced diabetic rats. After induction of chronic diabetes  with STZ, 10 mg RSV/kg per day was administered to DM and DM+RSV groups for  four weeks. At the end of the experiment, all rats were sacrificed and heart tissues  were stored at -80°C; mRNA expression levels of COX-1, COX-2, NF-κB, MMP-9, and SIRT1 genes were  analyzed with quantitative real-time PCR. We did not find any significant  effect of RSV on MMP-9, COX-1, COX-2, or NF-κB mRNA levels among the groups. However, SIRT1 mRNA  levels decreased in the DM group compared to controls and increased in the  DM+RSV group when compared to the DM group. SIRT1 is activated by RSV treatment  in diabetic heart tissue. Activation of SIRT1 by RSV may lead to a new  therapeutic approach for diabetic heart tissue. We conclude that RSV treatment  can alleviate heart dysfunction by inhibiton of inflammatory gene expression  such as SIRT1.
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Ankara, Turkey. atiyeseda@yahoo.com
authors.forename: A S
authors.initials: AS
authors.lastname: Yar
authors.forename: S
authors.initials: S
authors.lastname: Menevse
authors.forename: E
authors.initials: E
authors.lastname: Alp
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use; D009202:Cardiomyopathies / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology / Q000517:prevention & control*; D051545:Cyclooxygenase 1 / Q000235:genetics / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D012333:RNA, Messenger; D013267:Stilbenes; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D020780:Matrix Metalloproteinase 9; D056564:Sirtuin 1; D000077185:Resveratrol