title: Heat shock proteins and autophagy in rats with cerulein-induced acute pancreatitis.
abstract: OBJECTIVE
  Heat shock proteins (HSPs) protect rats from cerulein-induced acute pancreatitis (AP) by preventing the subcellular redistribution of cathepsin B and the activation of trypsinogen. Autophagy plays a critical role in the secretion of digestive enzymes and triggering of cerulein-induced AP via the colocalization of trypsinogen and lysosomes. Therefore, using a rat cerulein-induced AP model, we investigated whether HSPs prevent AP by regulating autophagy.
  
  
  METHODS
  Twelve hours after fed standard laboratory chow and water, the experimental groups (cerulein, water-immersion [WI]-cerulein and heat-shock [HS]-cerulein) and the control groups (control, WI, and HS) received one intraperitoneal injection of cerulein (50 µg/kg) or saline, respectively. All of the rats were sacrificed at 6 hours after injection. The severity of the AP was assessed based on the serum amylase level and the histological and electron microscopy findings. Western blotting was also performed for HSP60/70 and LC3B-II.
  
  
  RESULTS
  WI and HS induced HSP60 and HSP70, respectively. The induced HSP60/70 effectively prevented the development of cerulein-induced AP. Autophagy developed in the rats with cerulein-induced AP and was documented by the expression of LC3-II and electron microscopy findings. The WI-stressed rats and HS-treated rats did not develop cerulein-induced autophagy.
  
  
  CONCLUSIONS
  HSPs exert protective effects against cerulein-induced AP in rats by inhibiting autophagy.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Seoul Paik Hospital, Inje University College of Medicine, Seoul, Korea.
authors.forename: Jin Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Hong Sik
authors.initials: HS
authors.lastname: Lee
authors.forename: Soo Hyung
authors.initials: SH
authors.lastname: Ryu
authors.forename: You Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Jeong Seop
authors.initials: JS
authors.lastname: Moon
authors.forename: Chang Duck
authors.initials: CD
authors.lastname: Kim
authors.forename: In Youb
authors.initials: IY
authors.lastname: Chang
authors.forename: Sang Pill
authors.initials: SP
authors.lastname: Yoon