title: Relationship between increased albendazole systemic exposure and changes in single nucleotide polymorphisms on the β-tubulin isotype 1 encoding gene in Haemonchus contortus.
abstract: Resistance to benzimidazole anthelmintics in the nematode Haemonchus contortus has been correlated with single nucleotide polymorphisms (SNPs) on the β-tubulin isotype 1 gene. Three mutations can be used as markers for the detection of resistance, namely SNPs at position 200 and 167 (both TTC to TAC) or at position 198 (GAA to GCA). Harbouring a resistance genotype at any one of these codons can lead to a resistant phenotype. Our objective in this study was to analyse the frequencies of the three mutations when the albendazole dose rate and selection pressure were increased. We used adult H. contortus (males and females) collected directly from the abomasum of untreated lambs, or lambs treated with the manufacturer's recommended dose rate (5mg/kg), three times the recommended dose rate (15 mg/kg), or nine times the recommended dose rate (45 mg/kg). Anthelmintic efficacy was determined by worm and egg count reductions. For the surviving worms of the four treatment groups, the frequencies of each resistance SNP at codons 167, 200 and 198 were measured using pyrosequencing. Our results showed a strong relationship between an increasing dose rate and an increase in the frequency of the (TAC)(200) SNP and a decrease in the (TAC)(167) SNP. All worms genotyped were GAA at codon 198.
authors.affiliation: Institute of Parasitology, McGill University, Sainte Anne-de-Bellevue, Quebec, Canada H9X 3V9. virginie.barrere@mail.mcgill.ca
authors.forename: Virginie
authors.initials: V
authors.lastname: Barrère
authors.forename: Luis
authors.initials: L
authors.lastname: Alvarez
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Suarez
authors.forename: Laura
authors.initials: L
authors.lastname: Ceballos
authors.forename: Laura
authors.initials: L
authors.lastname: Moreno
authors.forename: Carlos
authors.initials: C
authors.lastname: Lanusse
authors.forename: Roger K
authors.initials: RK
authors.lastname: Prichard
mesh_terms: D015766:Albendazole / Q000494:pharmacology*; D000483:Alleles; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005838:Genotype; D006188:Haemonchiasis / Q000469:parasitology / Q000662:veterinary; D006189:Haemonchus / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012737:Sex Factors; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D014404:Tubulin / Q000145:classification* / Q000235:genetics*
chemical_list: D000871:Anthelmintics; D014404:Tubulin; D015766:Albendazole