title: Vitamin D supplementation during exercise training does not alter inflammatory biomarkers in overweight and obese subjects.
abstract: The purpose of this study was to examine the effects of vitamin D supplementation on inflammatory biomarkers in overweight and obese adults participating in a progressive resistance exercise training program. Twenty-three (26.1 ± 4.7 years) overweight and obese (BMI 31.3 ± 3.2 kg/m2) adults were randomized into a double-blind vitamin D supplementation (Vit D 4,000 IU/day; female 5, male 5) or placebo (PL, female 7; male 6) intervention trial. Both groups performed 12 weeks (3 days/week) of progressive resistance exercise training (three sets of eight exercises) at 70-80% of one repetition maximum. Whole-blood lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) α production as well as circulating C-reactive protein (CRP), TNFα, interleukin 6 (IL-6), and alanine aminotransferase (ALT) were assessed at baseline and after the 12-week intervention. No main effects of group or time were detected for circulating CRP, TNFα, IL-6, and ALT. As expected, when PL and Vit D groups were combined, there was a significant correlation between percent body fat and CRP at baseline (r = 0.45, P = 0.04), and between serum 25OHD and CRP at 12 weeks (r = 0.49, P = 0.03). The PL group had a significant increase in 25 μg/ml LPS + polymixin B-stimulated TNFα production (P = 0.04), and both groups had a significant reduction in unstimulated TNFα production (P < 0.05) after the 12-week intervention. Vitamin D supplementation in healthy, overweight, and obese adults participating in a resistance training intervention did not augment exercise-induced changes in inflammatory biomarkers.
authors.affiliation: Wastl Human Performance Laboratory, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Andres E
authors.initials: AE
authors.lastname: Carrillo
authors.forename: Michael G
authors.initials: MG
authors.lastname: Flynn
authors.forename: Catherine
authors.initials: C
authors.lastname: Pinkston
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Markofski
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Shawn S
authors.initials: SS
authors.lastname: Donkin
authors.forename: Dorothy
authors.initials: D
authors.lastname: Teegarden
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D003131:Combined Modality Therapy; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007196:Indiana; D018836:Inflammation Mediators / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood / Q000276:immunology / Q000628:therapy*; D050177:Overweight / Q000097:blood / Q000276:immunology / Q000628:therapy*; D055070:Resistance Training; D013997:Time Factors; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014807:Vitamin D; D002097:C-Reactive Protein; C104450:25-hydroxyvitamin D; D000410:Alanine Transaminase