title: [Expression of microRNA in extranodal NK/T cell lymphoma, nasal type].
abstract: OBJECTIVE
  To study the expression of microRNAs (miRNAs) in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT).
  
  
  METHODS
  TaqMan low density arrays were used to assess the expression level of 665 miRNAs in one case of EN-NK/T-NT and one normal nasopharyngeal tissue. Ninety-five miRNAs were found to be aberrantly expressed (86 being up-regulated and 9 being down-regulated) in EN-NK/T-NT, compared with normal nasopharyngeal tissue. The aberrant expression was found to be most significant in 8 miRNAs. According to the literature and miRNA database, the expression patterns of these 8 miRNAs were further analyzed in 15 cases of EN-NK/T-NT and 3 normal nasopharyngeal specimens, using the single tube TaqMan microRNA assays.
  
  
  RESULTS
  Three of the 8 miRNAs showed statistically significant difference in the expression in EN-NK/T-NT and normal nasopharyngeal specimens. These 3 miRNAs (miR-223, 886-3p and 34c-5p) were considered to play crucial roles in hemopoiesis, cellular proliferation and apoptosis. MiR-223 and miR-886-3p were significantly over-expressed in EN-NK/T-NT (P = 0.002, P = 0.010) while miR-34c-5p was significantly under-expressed (P = 0.017).
  
  
  CONCLUSIONS
  Certain types of miRNAs, especially those related to hemopoiesis, cellular proliferation and apoptosis, are aberrantly expressed in EN-NK/T-NT. The potential role of miRNAs in the molecular genetics of EN-NK/T-NT requires further investigation.
authors.affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Ti
authors.forename: Lin
authors.initials: L
authors.lastname: Nong
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D019002:CD56 Antigen / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D054391:Lymphoma, Extranodal NK-T-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D055815:Young Adult
chemical_list: D019002:CD56 Antigen; D035683:MicroRNAs; C525067:MIR223, human; C522918:NCAM1 protein, human