title: Detection of L-phenylalanine using molecularly imprinted solid-phase extraction and flow injection electrochemiluminescence.
abstract: A novel flow injection electrochemiluminescence method combined with molecularly imprinted solid-phase extraction was developed for the determination of L-phenylalanine, in which ${\rm{Ru(bpy}})_3^{2 + }$ was used as the luminophor and indium tin oxide glass was modified as the working electrode. Molecularly imprinted polymers, synthesized by self-assembly with functional monomer and crossing linker, were used for the selective extraction of L-phenylalanine. In order to overcome the drawbacks of traditional electrochemiluminescence cells such as high IR drop, high over-potential and so on, a novel electrochemiluminescence cell was developed. The enhanced electrochemiluminescence intensity was linearly related with the concentration of L-phenylalanine in the range from 1.0×10(-7) to 5.0×10(-5) g/mL with a detection limit of 2.59×10(-8) g/mL. The relative standard deviation for the determination of 1.0×10(-6) g/mL L-phenylalanine was 1.2% (n=11). The method showed higher sensitivity and good repeatability, and was successfully applied for the determination of L-phenylalanine in egg white, chicken and serum samples. A possible mechanism for the enhanced electrochemiluminescence response on indium tin oxide glass is proposed.
authors.affiliation: Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, P. R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Lu
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Fuwei
authors.initials: F
authors.lastname: Wan
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D000818:Animals; D002645:Chickens; D004529:Egg White / Q000737:chemistry; D055664:Electrochemical Techniques; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D009942:Organometallic Compounds; D010649:Phenylalanine / Q000032:analysis* / Q000097:blood; D011108:Polymers; D052616:Solid Phase Extraction / Q000379:methods*; D017971:Tin Compounds
chemical_list: D009942:Organometallic Compounds; D011108:Polymers; D017971:Tin Compounds; C103599:bis(bipyridyl)ruthenium(II); D010649:Phenylalanine; D015082:2,2'-Dipyridyl; C109984:indium tin oxide