title: Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa.
abstract: Phycobilisome (PBS) is a photosynthetic antenna supercomplex consisting of a central core subcomplex with several peripheral rods radiating from the core. Subunit structure of PBS was studied in a glaucocystophyte Cyanophora paradoxa strain NIES 547. Subunit composition of PBS was identified by N-terminal sequencing and genes for the subunits were determined by homology search of databases. They included rod linker proteins CpcK1 and CpcK2, rod-core linker proteins CpcG1 and CpcG2, and core linker proteins ApcC1 and ApcC2. Subfractionation by native polyacrylamide gel electrophoresis provided evidence for novel subcomplexes (ApcE/CpcK1/CpcG2/ApcA/ApcB/CpcD and ApcE/CpcK2/CpcG1/ApcA/ApcB), which connect rod and core subcomplexes. These skeleton-like structures may serve as a scaffold of the whole PBS assembly. Different roles of ApcC1 and ApcC2 were also suggested. Based on these findings, structural models for PBS were proposed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
authors.affiliation: Department of Life Sciences, University of Tokyo, Komaba, Tokyo, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Watanabe
authors.forename: Momoko
authors.initials: M
authors.lastname: Sato
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kondo
authors.forename: Rei
authors.initials: R
authors.lastname: Narikawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
mesh_terms: D000595:Amino Acid Sequence; D002499:Centrifugation, Density Gradient; D044424:Cyanophora / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D045524:Phycobilisomes / Q000737:chemistry*; D010802:Phylogeny; D021122:Protein Subunits
chemical_list: D045524:Phycobilisomes; D021122:Protein Subunits