title: Genetic and phenotypic characterization of sylvatic dengue virus type 4 strains.
abstract: Four serotypes of dengue virus (DENV 1-4) currently circulate between humans and domestic/peridomestic Aedes mosquitoes, resulting in 100 million infections per year. All four serotypes emerged, independently, from sylvatic progenitors transmitted among non-human primates by arboreal Aedes mosquitoes. This study investigated the genetic and phenotypic changes associated with emergence of human DENV-4 from its sylvatic ancestors. Analysis of complete genomes of 3 sylvatic and 4 human strains revealed high conservation of both the 5'- and 3'-untranslated regions but considerable divergence within the open reading frame. Additionally, the two ecotypes did not differ significantly in replication dynamics in cultured human liver (Huh-7), monkey kidney (Vero) or mosquito (C6/36) cells, although significant inter-strain variation within ecotypes was detected. These findings are in partial agreement with previous studies of DENV-2, where human strains produced a larger number of progeny than sylvatic strains in human liver cells but not in monkey or mosquito cells.
authors.affiliation: Center for Biodefense and Emerging Infectious Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0610, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Rossi
authors.forename: F
authors.initials: F
authors.lastname: Nasar
authors.forename: J
authors.initials: J
authors.lastname: Cardosa
authors.forename: S V
authors.initials: SV
authors.lastname: Mayer
authors.forename: R B
authors.initials: RB
authors.lastname: Tesh
authors.forename: K A
authors.initials: KA
authors.lastname: Hanley
authors.forename: S C
authors.initials: SC
authors.lastname: Weaver
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D002460:Cell Line; D003715:Dengue / Q000662:veterinary* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D060146:Ecotype; D019143:Evolution, Molecular; D005838:Genotype; D000882:Haplorhini; D006801:Humans; D007303:Insect Vectors / Q000821:virology; D058977:Molecular Sequence Annotation; D010641:Phenotype; D010802:Phylogeny; D018419:Primate Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins