title: Transcriptome analysis of H2O2-treated wheat seedlings reveals a H2O2-responsive fatty acid desaturase gene participating in powdery mildew resistance.
abstract: Hydrogen peroxide (H(2)O(2)) plays important roles in plant biotic and abiotic stress responses. However, the effect of H(2)O(2) stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H(2)O(2), we performed an mRNA tag analysis of wheat seedlings under 10 mM H(2)O(2) treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H(2)O(2) responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, 'transport' activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H(2)O(2)-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H(2)O(2)-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H(2)O(2) and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H(2)O(2) stress and uncovers potential links between H(2)O(2) and Bgt responses, hence providing important candidate genes for the PM resistance in wheat.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, MOA Key Lab for Germplasm and Biotechnology, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
authors.forename: Aili
authors.initials: A
authors.lastname: Li
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Lichuan
authors.initials: L
authors.lastname: Tang
authors.forename: Guangyao
authors.initials: G
authors.lastname: Zhao
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Zhu
authors.forename: Jinfang
authors.initials: J
authors.lastname: Chu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Zhang
authors.forename: Jizeng
authors.initials: J
authors.lastname: Jia
authors.forename: Long
authors.initials: L
authors.lastname: Mao
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000502:physiology*; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics; D020224:Expressed Sequence Tags; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014908:Triticum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D044943:Fatty Acid Desaturases