title: Facile and rapid magnetic relaxation switch immunosensor for endocrine-disrupting chemicals.
abstract: In order to develop facile, fast and sensitive detection methods for endocrine-disrupting chemicals (EDCs), we described a sensitive biosensing system involving magnetic relaxation switch, based on the assembly of cross-linked superparamagnetic iron oxide (CLIO) nanoparticles induced by the antigen-antibody biorecognition. The design of smart CLIO-based superparamagnetic iron oxide nanoparticles and antigen-OVA was described for the detection of bisphenol A [2,2-bis (4-hydroxyphenol) propane (BPA)]. The addition of BPA to the rapid magnetic relaxation switch immunosensor led to transverse relaxation time (T2) shortening compared to a blank control as shown by NMR relaxometry measurements. This process was also applied to the rapid and facile determination of concentrations of BPA in drinking water (tap water). Good linearity for all calibration curves was obtained, and the limit of detection (LOD) for BPA was 0.4 ng/mL in tap water.
authors.affiliation: School of Food Science & Technology, State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yan
authors.forename: Changlong
authors.initials: C
authors.lastname: Hao
authors.forename: Changrui
authors.initials: C
authors.lastname: Xing
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D001559:Benzhydryl Compounds; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D060766:Drinking Water / Q000032:analysis*; D052244:Endocrine Disruptors / Q000032:analysis* / Q000276:immunology; D005290:Ferric Compounds / Q000737:chemistry*; D007118:Immunoassay / Q000191:economics / Q000379:methods; D057230:Limit of Detection; D008280:Magnetics / Q000191:economics / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010636:Phenols / Q000032:analysis* / Q000276:immunology; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D060766:Drinking Water; D052244:Endocrine Disruptors; D005290:Ferric Compounds; D010636:Phenols; C000499:ferric oxide; C006780:bisphenol A; D000098826:Bisphenol A Compounds