title: Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways.
abstract: Cryptogein is a proteinaceous elicitor secreted by the oomycete Phytophthora cryptogea, which induces a hypersensitive response in tobacco plants. We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca(2+) dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca(2+) dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca(2+) dependent, as determined by their sensitivity to lanthanum chloride. The most Ca(2+)-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca(2+)-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco.
authors.affiliation: Université de Toulouse, Laboratoire de Recherches en Sciences Végétales, Castanet-Tolosan, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Amelot
authors.forename: François
authors.initials: F
authors.lastname: Dorlhac de Borne
authors.forename: Hélène
authors.initials: H
authors.lastname: San Clemente
authors.forename: Christian
authors.initials: C
authors.lastname: Mazars
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Grima-Pettenati
authors.forename: Christian
authors.initials: C
authors.lastname: Brière
mesh_terms: D020418:Algal Proteins; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D005656:Fungal Proteins; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D010838:Phytophthora; D059828:Plant Cells; D010935:Plant Diseases; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D059467:Transcriptome
chemical_list: D020418:Algal Proteins; D005656:Fungal Proteins; C061032:cryptogein protein, Phytophthora cryptogea; D002118:Calcium