title: [Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots].
abstract: Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon alpha2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants.
authors.forename: N A
authors.initials: NA
authors.lastname: Matveeva
authors.forename: E M
authors.initials: EM
authors.lastname: Kishchenko
authors.forename: A M
authors.initials: AM
authors.lastname: Shakhovskiĭ
authors.forename: A A
authors.initials: AA
authors.lastname: Potrokhov
authors.forename: N V
authors.initials: NV
authors.lastname: Kuchuk
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D060054:Agrobacterium / Q000235:genetics; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018651:Cichorium intybus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D003470:Culture Media; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D010957:Plasmids / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012038:Regeneration / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D016898:Interferon-alpha; D012333:RNA, Messenger; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis