title: Exploring new strains of dye-decolorizing bacteria.
abstract: This study unveiled a new strategy to explore new indigenous strains with excellent decolorization capabilities from freshwaters and seawaters. Two new bacterial decolorizers DX2b and SH7b, which have the capability to decolorize textile dyes, were isolated from Cross-Strait Taiwan and China. According to PCR-augmented 16S rRNA gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to type strains Rahnella aquatilis, Acinetobacter guillouiae, Microvirgula aerodenitrificans, and Pseudomonas sp. Time-series inspection upon azoreductase activity assay and generation of decolorized intermediates all confirmed in parallel with reductive decolorization of new decolorizers DX2b and SH7b. The result also showed that bacterial decolorization of these new strains was mainly catalyzed via the enzymatic expression of azoreductase and riboflavin reductase, and biosorption seemed not to play a crucial role color removal (approximately <10%).
authors.affiliation: School of Environmental and Material Engineering, 32 Qingquan Road, Laishan District , Yan-Tai University, Yantai 264005 China.
authors.forename: Jing-Long
authors.initials: JL
authors.lastname: Han
authors.forename: I-Son
authors.initials: IS
authors.lastname: Ng
authors.forename: Yanni
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuesong
authors.initials: X
authors.lastname: Zheng
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.forename: Chung-Chuan
authors.initials: CC
authors.lastname: Hsueh
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Liu
authors.forename: Bor-Yann
authors.initials: BY
authors.lastname: Chen
mesh_terms: D001391:Azo Compounds / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D004396:Coloring Agents / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013624:Taiwan
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D012336:RNA, Ribosomal, 16S; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase