title: Human papillomavirus detection in dysplastic and malignant oral verrucous lesions.
abstract: The role of human papillomaviruses (HPV) in dysplastic and malignant oral verrucous lesions is controversial since there is a wide range in the incidence of virus detection. This study used a multi-tiered method of HPV detection using DNA in-situ hybridisation (ISH) for low- and high-risk subtypes, consensus PCR, and HPV genotype analysis in archival tissue from 20 cases of dysplastic and malignant oral verrucous lesions. The biological significance of HPV DNA detection was assessed by p16 immunohistochemistry (IHC). While 1/7 carcinomas and 5/13 dysplasias contained HPV DNA by consensus PCR and genotype analysis, all specimens were negative for low- and high-risk HPV ISH and negative for p16 IHC. Results show that although high-risk HPV DNA is detectable in a subset of these lesions, the lack of p16 overexpression suggests that the oncogenic process is not driven by HPV oncoproteins.
authors.affiliation: Oral Pathology, Department of Clinical and Diagnostic Sciences, King's College London Dental Institute, London, UK.
authors.forename: Angela
authors.initials: A
authors.lastname: Stokes
authors.forename: Eliete
authors.initials: E
authors.lastname: Guerra
authors.forename: Jon
authors.initials: J
authors.lastname: Bible
authors.forename: Eugene
authors.initials: E
authors.lastname: Halligan
authors.forename: Guy
authors.initials: G
authors.lastname: Orchard
authors.forename: Edward
authors.initials: E
authors.lastname: Odell
authors.forename: Selvam
authors.initials: S
authors.lastname: Thavaraj
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D018289:Carcinoma, Verrucous / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral