title: 14-aminotetradecanoic acid exhibits antioxidant activity and ameliorates xenobiotics-induced cytotoxicity.
abstract: Natural compounds with free-radical scavenging activity have potential role in maintaining human health and preventing diseases. In this study, we report the antioxidant and cytoprotective properties of 14-aminotetradecanoic acid (ATDA) isolated from the Decalepis hamiltonii roots. ATDA is a potent scavenger of superoxide (O(2) (•-)), hydroxyl ((•)OH), nitric oxide ((•)NO), and lipid peroxide (LOO(•)) physiologically relevant free radicals with IC(50) values in nM (36-323) range. ATDA also exhibits concentration-dependent secondary antioxidant activities like reducing power, metal-chelating activity, and inhibition of protein carbonylation. Further, ATDA at nM concentration prevented CuSO(4)-induced human LDL oxidation. ATDA demonstrated cytoprotective activity in primary hepatocytes and Ehrlich ascites tumor cells against oxidative stress inducing xenobiotics apart from the in vitro free-radical scavenging activity. The mechanism of cytoprotective action involved maintaining the intracellular glutathione, scavenging of reactive oxygen species, and inhibition of lipid peroxidation. It is suggested that ATDA is a novel bioactive molecule with potential health implications.
authors.affiliation: Department of Food Protectants and Infestation Control, Central Food Technological Research Institute, Mysore, 570020 Karnataka, India. anup@uab.edu
authors.forename: Anup
authors.initials: A
authors.lastname: Srivastava
authors.forename: L Jagan Mohan
authors.initials: LJ
authors.lastname: Rao
authors.forename: T
authors.initials: T
authors.lastname: Shivanandappa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry; D019610:Cytoprotection; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects; D019814:Myristic Acid / Q000737:chemistry / Q000494:pharmacology*; D009569:Nitric Oxide / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017382:Reactive Oxygen Species / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry; D015262:Xenobiotics / Q000633:toxicity
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D015262:Xenobiotics; D019814:Myristic Acid; D013481:Superoxides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D005978:Glutathione