title: Destructive effects of butyrate on the cell envelope of Helicobacter pylori.
abstract: Helicobacter pylori can be found in the oral cavity and is mostly detected by the use of PCR techniques. Growth of H. pylori is influenced by various factors in the mouth, such as the oral microflora, saliva and other antimicrobial substances, all of which make colonization of the oral cavity by H. pylori difficult. In the present study, we analysed the effect of the cell supernatant of a representative periodontal bacterium Porphyromonas gingivalis on H. pylori and found that the cell supernatant destroyed the H. pylori cell envelope. As P. gingivalis produces butyric acid, we focused our research on the effects of butyrate and found that it significantly inhibited the growth of H. pylori. H. pylori cytoplasmic proteins and DNA were detected in the extracellular environment after treatment with butyrate, suggesting that the integrity of the cell envelope was compromised and indicating that butyrate has a bactericidal effect on H. pylori. In addition, levels of extracellular H. pylori DNA increased following treatment with the cell supernatant of butyric acid-producing bacteria, indicating that the cell supernatant also has a bactericidal effect and that this may be due to its butyric acid content. In conclusion, butyric acid-producing bacteria may play a role in affecting H. pylori colonization of the oral cavity.
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Yonezawa
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Osaki
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hanawa
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kurata
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Zaman
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Timothy Derk Hoong
authors.initials: TDH
authors.lastname: Woo
authors.affiliation: Miyarisan Pharmaceutical Co. Ltd, Tokyo, Japan.|Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Motomichi
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Laboratory for Electron Microscopy, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Sachie
authors.initials: S
authors.lastname: Matsubara
authors.affiliation: Department of Anatomy, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.affiliation: Department of Bacteriology, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo 181-8611, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002087:Butyrates / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D046972:Clostridium butyricum / Q000737:chemistry / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D016480:Helicobacter pylori / Q000166:cytology* / Q000187:drug effects*; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002087:Butyrates; D004269:DNA, Bacterial