title: Hsp90 interacts with AMPK and mediates acetyl-CoA carboxylase phosphorylation.
abstract: Heat shock protein 90 (Hsp90) serves to stabilise and correctly fold multiple significant client proteins associated with cell proliferation and cell survival. However, little is known about the Hsp90 client proteins that regulate cell metabolism. Here, we describe a unique ability of Hsp90 to regulate the stability and activity of AMP-activated kinase (AMPK), a key sensor of cellular energy status. Hsp90 is found to interact with AMPK and to maintain its AMP-activated kinase activity, which in turn is required for the phosphorylation of its substrate, acetyl-CoA carboxylase (ACC), the key enzyme in fatty acid metabolism. Our binding analysis reveals that both the γ subunit and the α subunit of AMPK bind to Hsp90 with a high affinity. We demonstrate that Hsp90 inhibitors, including geldanamycin (GA) and mycoepoxydiene (MED), can induce the dissociation of AMPK from Hsp90, and cause a significant decrease in phosphorylation of AMPK and ACC. Furthermore, we demonstrate that shRNAs of Hsp90 can efficiently suppress the activation of AMPK. These findings not only establish a novel interaction between Hsp90 and AMPK but also suggest a new mechanism for regulating tumour cell fatty acid metabolism.
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen 361005, China. ru898@xmu.edu.cn
authors.forename: Lianru
authors.initials: L
authors.lastname: Zhang
authors.forename: Yuting
authors.initials: Y
authors.lastname: Yi
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Guo
authors.forename: Yifei
authors.initials: Y
authors.lastname: Sun
authors.forename: Shun
authors.initials: S
authors.lastname: Ma
authors.forename: Shuyan
authors.initials: S
authors.lastname: Xiao
authors.forename: Jing
authors.initials: J
authors.lastname: Geng
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Zheng
authors.forename: Siyang
authors.initials: S
authors.lastname: Song
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism*; D016227:Benzoquinones / Q000494:pharmacology; D001665:Binding Sites; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D050356:Lipid Metabolism / Q000235:genetics*; D008958:Models, Molecular; D010766:Phosphorylation; D010957:Plasmids; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011753:Pyrones / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D014162:Transfection
chemical_list: D016227:Benzoquinones; D001952:Bridged-Ring Compounds; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011753:Pyrones; D034741:RNA, Small Interfering; C476085:mycoepoxydiene; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases; D000103:Acetyl-CoA Carboxylase; C001277:geldanamycin