title: DNA- and RNA-binding and enhanced DNA-photocleavage properties of a ferrocenyl-containing ruthenium(II) complex.
abstract: The interaction of [Ru(bpy)(2)(fip)](PF(6))(2) {bpy=2,2'-bipyridine, fip=2-ferrocenyl-1H-imidazo[4,5-f][1,10]-phenanthroline} with calf thymus DNA and yeast tRNA was investigated comparatively by UV-visible absorption and luminescence spectrophotometric titrations, steady-state emission quenching by [Fe(CN)(6)](4-), ethidium bromide competition experiment, DNA thermal denaturation, viscosity measurements and salt effect studies. The results suggest that the complex binds to the DNA more strongly than to the RNA. The density functional theory calculations were also carried out in order to better understand the nucleic acid binding properties. Agarose gel electrophoresis showed that the complex exhibited enhanced DNA-photocleavage capacity on pUC 18 plasmid DNA under irradiation at 360 nm as compared with a ferrocenyl-free analogous complex.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhao
authors.forename: Mei-Jiao
authors.initials: MJ
authors.lastname: Han
authors.forename: An-Guo
authors.initials: AG
authors.lastname: Zhang
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Wang
mesh_terms: D001667:Binding, Competitive; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D053837:DNA Cleavage; D004278:DNA, Superhelical / Q000737:chemistry; D024202:Electrophoretic Mobility Shift Assay; D004996:Ethidium / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D000075163:Metallocenes; D009994:Osmolar Concentration; D010782:Photolysis; D010957:Plasmids / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry* / Q000528:radiation effects; D012343:RNA, Transfer / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D017186:Titrimetry; D044366:Transition Temperature; D014466:Ultraviolet Rays; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D004278:DNA, Superhelical; D005296:Ferrous Compounds; D007364:Intercalating Agents; D000075163:Metallocenes; D012331:RNA, Fungal; D012428:Ruthenium; D004247:DNA; D012343:RNA, Transfer; C427203:calf thymus DNA; D004996:Ethidium; C004998:ferrocene