title: [Expression of galectin-1 in carcinogenesis of oral mucosal epithelium].
abstract: OBJECTIVE
  To investigate the expression of galectin-1 in oral squamous cell carcinoma(OSCC) and its clinical significance.
  
  
  METHODS
  Detection of the mRNA and protein expression of galectin-1 in the in vitro cellular carcinogenesis model of OSCC, OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blotting and immunohistochemistry, respectively.
  
  
  RESULTS
  The value of galectin-1 mRNA and protein level in human immortalized oral epithelia cell (HIOEC) cell was 0.071 ± 0.023, 0.118 ± 0.046, Compared with the HIOEC, galectin-1 mRNA level and protein expression were increased significantly in all the cell lines (0.141 ± 0.049, 0.504 ± 0.33) (P < 0.01). The levels of mRNA and protein expression of galectin-1 were significantly higher in the cancerous tissue (0.059 ± 0.034, 1.5 ± 0.68) than in the normal adjacent tissues (0.029 ± 0.012, 0.4 ± 0.56) (P < 0.01).
  
  
  CONCLUSIONS
  The expression of galectin-1 gene up-regulated in carcinogenesis process of OSCC significantly may be related to the tumorigenesis and development of OSCC, which illustrates its potential clinical application as tumor marker for early diagnosis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, China.
authors.forename: Kui-Jie
authors.initials: KJ
authors.lastname: Wei
authors.forename: Hong-Ya
authors.initials: HY
authors.lastname: Pan
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Lai-ping
authors.initials: LP
authors.lastname: Zhong
authors.forename: Dong-Xia
authors.initials: DX
authors.lastname: Ye
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D037483:Galectin 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D037483:Galectin 1; C445499:LGALS1 protein, human; D012333:RNA, Messenger