title: Genomic sequence analysis of four new chrysanthemum virus B isolates: evidence of RNA recombination.
abstract: Chrysanthemums worldwide suffer from a high incidence of infection with chrysanthemum virus B (CVB), a member of the genus Carlavirus, family Betaflexiviridae. Three major lineages or strains of this virus have been found in India, but none have been characterized beyond the genetic variation they display in their coat protein genes. Here, we describe the analysis of four near-complete genome sequences (from the three lineages) representing the genetic diversity of these strains. Ranging in size from 8815 to 8855 nucleotides (excluding the polyA tail), these four isolates have a genome organization very similar to that of the recently reported Japanese isolate of CVB, with which they share between 70 and 73% genome-wide sequence identity. We present further evidence that recombination may feature quite prominently in the evolution of CVB.
authors.affiliation: Plant Virus Laboratory, Council of Scientific and Industrial Research, Institute of Himalayan Bioresource Technology, Palampur, 176061 HP, India.
authors.forename: Lakhmir
authors.initials: L
authors.lastname: Singh
authors.forename: Vipin
authors.initials: V
authors.lastname: Hallan
authors.forename: D P
authors.initials: DP
authors.lastname: Martin
authors.forename: Raja
authors.initials: R
authors.lastname: Ram
authors.forename: A A
authors.initials: AA
authors.lastname: Zaidi
mesh_terms: D017788:Carlavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D031202:Chrysanthemum / Q000821:virology*; D016000:Cluster Analysis; D019143:Evolution, Molecular; D023061:Gene Order; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny; D014777:Virus Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral