title: Photodynamic therapy leads to death of C6 glioma cells partly through AMPAR.
abstract: Glioma cells release glutamate during growth, which promotes proliferation and migration of itself and causes excitotoxicity to the surrounding neurons by Ca(2+) influx through glutamate receptors. However, the role of glutamate and its receptors in the photodynamic therapy (PDT) on glioma cells is still unclear. Here we administered α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid glutamate receptor (AMPAR) antagonist prior to PDT on C6 glioma cells. The changes of glutamate release, expression of AMPAR, apoptosis of C6 cells, and intracellular free calcium ([Ca(2+)](i)) were examined after PDT. We found that PDT increased extracellular glutamate and expression of AMPAR subunit GluR1 and GluR2, which might result in Ca(2+) influx and apoptosis of C6 cells. When AMPAR antagonist was added, intracellular free calcium reduced and apoptosis rate of C6 cells decreased. These results indicate that PDT may lead to death of C6 glioma cells partly through glutamate and its receptors AMPAR, which induces Ca(2+) influx and then cells apoptosis. This study allows us to further understand the effects and molecular mechanism of PDT on glioma.
authors.affiliation: Department of Neurosurgery, Southwest Hospital, Third Military Medical University, No. 29, GaoTanYan street, Chong-Qing 400038, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Shengli
authors.initials: S
authors.lastname: Hu
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Cheng
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Yi
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D010778:Photochemotherapy; D018091:Receptors, AMPA / Q000819:agonists / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology*
chemical_list: D018091:Receptors, AMPA; D018698:Glutamic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid