title: Evaluation of antioxidant and urease inhibition activities of roots of Glycyrrhiza glabra.
abstract: The object of this study is to determine the antioxidant activity of extracts from Glycyrrhiza glabra roots. The parent extract is methanolic extract while its sub fractions were prepared in ethyl acetate, chloroform, and n-butanol. The method based on scavenging activity and reduction capability of 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH). Urease inhibition activities of these extracts were also evaluated. Chloroform fraction was the most effective antioxidant with 87.7% activity but the activity is less than the crude methanolic extract i.e. 90%. Chloroform fraction showed the same trend in reducing power as that in radical scavenging activity. However n- butanol extract was devoid of any activity when compared to standard BHA. Crude methanolic fraction and its sub-fractions were also screened for enzyme inhibition activities using jackbean urease as substrate. Significant anti urease activity i.e. 72 % was observed in the ethyl acetate fraction with respect to standard inhibitor thiourea.
authors.affiliation: Pharmaceutical Research Centre, PCSIR Labs. Complex, Karachi, Pakistan.
authors.forename: Mehreen
authors.initials: M
authors.lastname: Lateef
authors.forename: Lubna
authors.initials: L
authors.lastname: Iqbal
authors.forename: Nudrat
authors.initials: N
authors.lastname: Fatima
authors.forename: Kauser
authors.initials: K
authors.lastname: Siddiqui
authors.forename: Nighat
authors.initials: N
authors.lastname: Afza
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zia-ul-Haq
authors.forename: Mansoor
authors.initials: M
authors.lastname: Ahmad
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006035:Glycyrrhiza / Q000737:chemistry*; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D010936:Plant Extracts; D014510:Urease