title: Analysis of neovascularization after implanting porous silk fibroin films in rats.
abstract: The purpose of this paper is to explore neovascularization in the biomaterials implanted in vivo. Capillaries formation of dermis defect filled with porous silk fibroin films (PSFFs) implant were investigated in rat at different times. Vascular endothelial growth factor (VEGF), CD34 and hypoxia inducible factor-1 (HIF) were observed in inflammatory cells and fibroblast in PSFFs by day 1, but expression in HIF and VEGF decreased by day 7, otherwise VEGF expressed in vascular endothelial cell increased by day 15 and 25, respectively. VEGF, angiopoietin 1 (Ang-1) and integrins αv and integrins β3 in PSFFs were observed by Reverse transcription-polymerase chain reaction (RT-PCR). Polyvinyl alcohol (PVA) sponge was control. PSFFs have better neovascularization than that of PVA expression of VEGF, HIF and angiogenesis in immunohistochemical and mRNA level.
authors.affiliation: National Engineering Laboratory for Modern Silk, Suzhou, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Sun
authors.forename: Lun
authors.initials: L
authors.lastname: Bai
mesh_terms: D042683:Angiopoietin-1 / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology*; D051793:Hypoxia-Inducible Factor 1 / Q000032:analysis*; D039564:Integrin alphaV / Q000378:metabolism; D039661:Integrin beta3 / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000737:chemistry / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D042683:Angiopoietin-1; D018952:Antigens, CD34; D001672:Biocompatible Materials; D051793:Hypoxia-Inducible Factor 1; D039564:Integrin alphaV; D039661:Integrin beta3; D012333:RNA, Messenger; D047011:Silk; D042461:Vascular Endothelial Growth Factor A; D005349:Fibroins