title: Purification and characterization of a novel RNase with antiproliferative activity from the mushroom Lactarius flavidulus.
abstract: A 14.6-kDa RNase, with a pH optimum of 5.5 and a temperature optimum of 70 °C, was isolated from dried fruiting bodies of the edible mushroom Lactarius flavidulus. The purification procedure involved, in succession, ion exchange chromatography on DEAE-cellulose, CM-cellulose and SP-Sepharose, and finally FPLC-gel filtration on Superdex 75. The RNase was adsorbed on all three ion exchangers. The ranking of its activity toward various polyhomoribonucleotides was poly(C) > poly(G) > poly(A) > poly(U). It suppressed proliferation of HepG2 cells and L1210 cells with an IC₅₀ of 3.19 μM and 6.52 μM, respectively. It also inhibited the activity of HIV-1 reverse transcriptase with an IC₅₀ of 2.55 μM.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: Tzibun
authors.initials: T
authors.lastname: Ng
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048690:Fruiting Bodies, Fungal / Q000201:enzymology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D012260:Ribonucleases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013696:Temperature
chemical_list: C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D012260:Ribonucleases