title: Secretory production of cell wall components by Saccharomyces cerevisiae protoplasts in static liquid culture.
abstract: When protoplasts of Saccharomyces cerevisiae T7 and IFO 0309 are cultured in a static liquid culture at 2.5 × 10(6) protoplasts/ml, cell wall regeneration does not occur and cell wall components (CWC) are released into the culture broth. By using a specialized fluorometer, the concentrations of CWC could be measured on the basis of the fluorescence intensity of the CWC after staining with Fluostain I. The inoculum concentration, pH, and osmotic pressure of the medium were important factors for the production of CWC in culture. Under optimal culture conditions, S. cerevisiae T7 protoplasts produced 0.91 mg/ml CWC after 24 h. The CWC induced the tumor necrosis factor-α production about 1.3 times higher than that of the commercially available β-1,3/1,6-glucan from baker's yeast cells.
authors.affiliation: Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan. aoyagi@sakura.cc.tsukuba.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Aoyagi
authors.forename: Mikiko
authors.initials: M
authors.lastname: Ishizaka
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009997:Osmotic Pressure; D011523:Protoplasts / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003470:Culture Media