title: Expression of infection-related genes in parasites and host during murine experimental infection with Leishmania (Leishmania) amazonensis.
abstract: Leishmania parasites are able to interfere with host immune responses on many levels, as T cell responses balance, as observed in the murine model of infection. In the present study, we analyzed genes expression in both host and parasite during the progression of infection. Host genes associated to T-lymphocytes responses, MHC classes I and II, as well as parasite enzymes genes, cysteine-proteinases (CP) B and C, were examined in mice along evolution of infection by Leishmania (Leishmania) amazonensis. Murine strains with distinct levels of susceptibility to infection presented different patterns of MHC genes expression: MHC class I genes tend to have higher expression levels in CBA mice, whereas MHC class II genes expression predominates in BALB/c mice. CPB genes expression in the parasites was shown to predominate over CPC in both mice strains tested. Understanding genes expression patterns during infection may lead to new and more efficient treatments for leishmaniasis.
authors.affiliation: Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Pavilhão Leonidas Deane- sala 209, Avenida Brasil, 4365 Manguinhos, Rio de Janeiro/RJ, CEP 21040-360, Brasil. bpereira@ioc.fiocruz.br
authors.forename: Bernardo Acácio Santini
authors.initials: BA
authors.lastname: Pereira
authors.forename: Constança
authors.initials: C
authors.lastname: Britto
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Alves
mesh_terms: D000818:Animals; D057056:Cysteine Proteases / Q000096:biosynthesis; D004195:Disease Models, Animal; D015870:Gene Expression; D020869:Gene Expression Profiling; D006649:Histocompatibility Antigens / Q000096:biosynthesis; D054884:Host-Pathogen Interactions; D007894:Leishmania mexicana / Q000235:genetics* / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA
chemical_list: D006649:Histocompatibility Antigens; D057056:Cysteine Proteases