title: Assessment of autophagosome formation by transmission electron microscopy.
abstract: Autophagy is a complex degradative process by which cytosolic material, including organelles, is randomly sequestered within double-membrane bound vesicles termed autophagosomes and targeted for degradation. Initially described as a nutrient stress adaptation response, the process of autophagy is now recognized as a central mechanism involved in many developmental processes. In this chapter, we provide guidelines to assess the initial steps of autophagy by monitoring autophagic body vacuolar accumulation. We employed a standard electron microscopy approach to observe the vacuoles of nutrient stressed fungal cells.
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Marina
authors.initials: M
authors.lastname: Nadal
authors.forename: Scott E
authors.initials: SE
authors.lastname: Gold
mesh_terms: D001343:Autophagy; D005658:Fungi / Q000166:cytology / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods