title: A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons.
abstract: Random mutagenesis followed by selection or screening is a commonly used strategy to improve protein function. Despite many available methods for random mutagenesis, nearly all generate mutations at the nucleotide level. An ideal mutagenesis method would allow for the generation of 'codon mutations' to change protein sequence with defined or mixed amino acids of choice. Herein we report a method that allows for mutations of one, two or three consecutive codons. Key to this method is the development of a Mu transposon variant with asymmetric terminal sequences. As a demonstration of the method, we performed multi-codon scanning on the gene encoding superfolder GFP (sfGFP). Characterization of 50 randomly chosen clones from each library showed that more than 40% of the mutants in these three libraries contained seamless, in-frame mutations with low site preference. By screening only 500 colonies from each library, we successfully identified several spectra-shift mutations, including a S205D variant that was found to bear a single excitation peak in the UV region.
authors.affiliation: Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: T Ashton
authors.initials: TA
authors.lastname: Cropp
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004251:DNA Transposable Elements / Q000235:genetics*; D019020:Directed Molecular Evolution / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D049452:Green Fluorescent Proteins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D049452:Green Fluorescent Proteins