title: Establishing efficient xenograft models with intrinsic vascularisation for growing primary human low-grade sarcomas.
abstract: BACKGROUND
  There are no xenograft models of low-grade soft tissue sarcoma. Transplant survival remains an obstacle in sarcoma xenograft models and is attributed to post-transplantation hypoxia. Models with an intrinsic tissue - engineered vascular supply may overcome this obstacle. The aim of this study was to establish a novel xenograft model of primary human low grade soft tissue sarcoma.
  
  
  METHODS
  Primary low-grade liposarcoma fragments were transplanted into a silicon chamber, placed around the superficial epigastric vessels in athymic nude mice. Xenograft samples were assessed histologically (light/electron microscopy and immunohistochemistry for S100).
  
  
  RESULTS
  All xenotransplants of low grade primary soft tissue liposarcoma (n=4) engrafted, led to the development of solid tumours in mice. Histological and immunohistochemical staining confirmed the xenografts as being well-differentiated liposarcomas identical to the original tumor tissue.
  
  
  CONCLUSIONS
  Successful transplantation of human low-grade liposarcoma tissue in mice was established for the first time using a model with an intrinsic vascular supply.
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, 44789 Bochum, North Rhine Westphalia, Germany. d.tilkorn@web.de
authors.forename: Daniel
authors.initials: D
authors.lastname: Tilkorn
authors.forename: Adrien
authors.initials: A
authors.lastname: Daigeler
authors.forename: Ingo
authors.initials: I
authors.lastname: Stricker
authors.forename: Angela
authors.initials: A
authors.lastname: Schaffran
authors.forename: Inge
authors.initials: I
authors.lastname: Schmitz
authors.forename: Lars
authors.initials: L
authors.lastname: Steinstraesser
authors.forename: Joerg
authors.initials: J
authors.lastname: Hauser
authors.forename: Andrej
authors.initials: A
authors.lastname: Ring
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Steinau
authors.forename: Sammy
authors.initials: S
authors.lastname: Al-Benna
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D004195:Disease Models, Animal; D006801:Humans; D000860:Hypoxia; D007150:Immunohistochemistry / Q000379:methods; D008080:Liposarcoma / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron / Q000379:methods; D009368:Neoplasm Transplantation / Q000379:methods*; D009389:Neovascularization, Pathologic; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology