title: Rab30 is required for the morphological integrity of the Golgi apparatus.
abstract: BACKGROUND
  Rab GTPases are key coordinators of eukaryotic intracellular membrane trafficking. In their active states, Rabs localise to the cytoplasmic face of intracellular compartments where they regulate membrane trafficking processes. Many Rabs have been extensively characterised whereas others, such as Rab30, have to date received relatively little attention.
  
  
  RESULTS
  Here, we demonstrate that Rab30 is primarily associated with the secretory pathway, displaying predominant localisation to the Golgi apparatus. We find by time-lapse microscopy and fluorescence recovery after photobleaching studies that Rab30 is rapidly and continuously recruited to the Golgi. We also show that Rab30 function is required for the morphological integrity of the Golgi. Finally, we demonstrate that inactivation of Rab30 does not impair anterograde or retrograde transport through the Golgi.
  
  
  CONCLUSIONS
  Taken together, these data illustrate that Rab30 primarily localises to the Golgi apparatus and is required for the structural integrity of this organelle.
authors.affiliation: Molecular Cell Biology Laboratory, Department of Biochemistry, BioSciences Institute, University College Cork, Cork, Ireland.
authors.forename: Eoin E
authors.initials: EE
authors.lastname: Kelly
authors.forename: Francesca
authors.initials: F
authors.lastname: Giordano
authors.forename: Conor P
authors.initials: CP
authors.lastname: Horgan
authors.forename: Florence
authors.initials: F
authors.lastname: Jollivet
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
authors.forename: Mary W
authors.initials: MW
authors.lastname: McCaffrey
mesh_terms: D003600:Cytosol / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching; D015870:Gene Expression; D020868:Gene Silencing; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010957:Plasmids; D021381:Protein Transport / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D059008:Time-Lapse Imaging; D014162:Transfection; D020691:rab GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; C101860:RAB30 protein, human; D020691:rab GTP-Binding Proteins