title: Computational studies on a new cationic peroxidase isoenzyme from artichoke leaves.
abstract: Previously we presented the purification, biochemical characterization, and cloning of a cationic peroxidase isoenzyme (CysPrx) from artichoke (Cynara cardunculus subsp scolymus (L.) Hegi) leaves. The protein was shown to have some interesting properties, suggesting that CysPrx could be a considered as a potential candidate for industrial application. In addition, from the CysPrx sequence, two full-lengh cDNAs: CysPrx1 and CysPrx2, differing for three amino acids, were isolated. A three-dimensional model was predicted from CysPrx1 by homology modeling, using two different computational tools. Herein we discuss the roles of particular amino acid residues and structural motifs or regions of both deduced sequences with the aim to find new understandings between the new plant peroxidase isoenzymes and their physiological substrates. Additionally, the obtained information may lead to new methods for improving the stability of the enzyme in several processes of biotechnological interest for peroxidase applications.
authors.affiliation: Institute of Sciences of Food Production-CNR, Bari, Italy. angela.cardinali@ispa.cnr.it
authors.forename: Angela
authors.initials: A
authors.lastname: Cardinali
authors.forename: Isabella
authors.initials: I
authors.lastname: D'Antuono
authors.forename: Benny Danilo
authors.initials: BD
authors.lastname: Belviso
authors.forename: Rocco
authors.initials: R
authors.lastname: Caliandro
mesh_terms: D019295:Computational Biology / Q000379:methods*; D031943:Cynara scolymus / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D010544:Peroxidases