title: Contribution of the residual body in the spatial organization of Toxoplasma gondii tachyzoites within the parasitophorous vacuole.
abstract: Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization.
authors.affiliation: Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Avenue IPN No 2508, San Pedro Zacatenco, Gustavo A. Madero 07360 Mexico City, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Muñiz-Hernández
authors.forename: M González del
authors.initials: MG
authors.lastname: Carmen
authors.forename: M
authors.initials: M
authors.lastname: Mondragón
authors.forename: C
authors.initials: C
authors.lastname: Mercier
authors.forename: M F
authors.initials: MF
authors.lastname: Cesbron
authors.forename: S L
authors.initials: SL
authors.lastname: Mondragón-González
authors.forename: S
authors.initials: S
authors.lastname: González
authors.forename: R
authors.initials: R
authors.lastname: Mondragón
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D049109:Cell Proliferation; D004285:Dogs; D008018:Life Cycle Stages; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000254:growth & development / Q000648:ultrastructure*; D014123:Toxoplasmosis / Q000378:metabolism / Q000473:pathology*; D014617:Vacuoles / Q000469:parasitology / Q000648:ultrastructure