title: Proteomic analysis shows differential protein expression in endothelial progenitor cells between healthy subjects and ischemic stroke patients.
abstract: OBJECTIVE
  An increase in the circulating concentration of endothelial progenitor cells (EPCs) is associated with a better outcome in patients with acute ischemic stroke. Likewise, EPCs are heterogeneous cells, with functional differences and different protein  expressions. Our objective was to compare protein expressions of EPCs from ischemic stroke patients and healthy subjects.
  
  
  METHODS
  Eleven ischemic stroke patients and 11 healthy subjects, matched by age and gender, were included in this study. EPC colonies were defined as early outgrowth  colony forming unit-endothelial cell. Cells were lysed and proteins were purified and separated on two-dimensional gels. Gel images were analyzed using the PDQuest software and protein differences between EPCs from ischemic stroke patients and healthy subjects were identified by mass spectrometry.  Results were finally validated by western blot.
  
  
  RESULTS
  Proteomic analysis revealed three qualitative differences between EPCs from healthy subjects and ischemic stroke patients. Two of them, endoplasmatic reticulum protein-29 and CdC-42, were only expressed in EPCs from healthy  subjects, whereas elongation factor-2 was only identified in EPCs from ischemic stroke patients. Furthermore, we identified one protein, peroxiredoxin-1, whose expression was 10 times stronger in ischemic stroke patients than in healthy subjects. Western blot analysis showed greater expression  of endoplasmatic reticulum protein-29 in EPCs from healthy subjects and elongation factor-2 and peroxiredoxin-1 in EPCs from ischemic stroke patients.
  
  
  CONCLUSIONS
  Proteomic analysis showed differences in protein expressions of EPCs from ischemic stroke patients and healthy subjects  that may be involved in mechanisms related to functional impairment.
authors.affiliation: Hospital Clínico Universitario, IDIS, University of Santiago de Compostela, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Brea
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodríguez-González
authors.forename: Tomás
authors.initials: T
authors.lastname: Sobrino
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Yañez
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanco
authors.forename: José
authors.initials: J
authors.lastname: Castillo
mesh_terms: D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000172:deficiency; D006801:Humans; D008297:Male; D061251:Primary Cell Culture; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods* / Q000592:standards; D012016:Reference Values; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D020521:Stroke / Q000378:metabolism* / Q000503:physiopathology
chemical_list: C491738:ERP29 protein, human; D006360:Heat-Shock Proteins; D011506:Proteins