title: Molecular characterization of Calocedrus  rupestris Aver., H.T. Nguyen & L.K. Phan, 2008 (Cupressaceae) based on  ITS1 partial sequence.
abstract: Calocedrus  rupestris Aver., H.T. Nguyen & L.K. Phan was described in  2008 based on some morphological characters that were not sufficiently  significant to discriminate it as a species distinct from C. macrolepis Kurz.  We applied a new approach to resolve these conflicting views by using sequence  data from DNA (ITS) to elucidate phylogenetic relationships between the two  species. Analyses of a partial ITS1 sequence in 5 individuals of 2  subpopulations of C. macrolepis and 18 individuals of 8 subpopulations  of C. rupestris collected in Vietnam were done. Molecular  characterization of the two species showed its low divergence with the lack of  autapomorphic characters. In addition, the ITS1 partial sequences of some C.  rupestris individuals were identical with C. macrolepis. Due to the  less distinctive morphology between C. rupestris and C. macrolepis,  the divergence between them does not exceed the interspecific levels, and  therefore, C. rupestris could not be regarded as an independent species  in relation to C. macrolepis but only as one of its varieties, C.  macrolepis var. rupestris (Aver., H.T. Nguyen & L.K. Phan) L.K.  Phan, Long K. Phan & Aver.
authors.affiliation: Vietnam National Museum of Nature, Hanoi, Vietnam. pklong@vast.ac.vn
authors.forename: P K
authors.initials: PK
authors.lastname: Long
authors.forename: N T P
authors.initials: NT
authors.lastname: Trang
authors.forename: L V
authors.initials: LV
authors.lastname: Averyanov
authors.forename: P K
authors.initials: PK
authors.lastname: Loc
mesh_terms: D001483:Base Sequence; D029779:Cupressaceae / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer