title: Iodide treatment acutely increases pendrin (SLC26A4) mRNA expression in the rat thyroid and the PCCl3 thyroid cell line by transcriptional mechanisms.
abstract: Iodine is a critical element involved in thyroid hormone synthesis. Its efflux into the follicular lumen is thought to occur, in part, through pendrin at the apical membrane of thyrocytes. This study attempted to investigate whether iodide administration affects SLC26A4 mRNA expression in rat thyroid and in PCCl3 cells. Rats and cells were treated or not with NaI from 30 min up to 48 h. One group was concomitantly treated with sodium perchlorate. SLC26A4 mRNA expression was also investigated in PCCl3 cells treated with actinomycin D prior to NaI treatment. Iodide administration significantly increased SLC26A4 mRNA content in both models. The simultaneous administration of NaI and perchlorate, as well as the treatment of PCCl3 cells with actinomycin D prevented this effect, indicating that intracellular iodide is essential for this event, which appears to be triggered by transcriptional mechanisms. These data show that intracellular iodide rapidly upregulates SLC26A4 mRNA expression.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, 05508-000 São Paulo, SP, Brazil.
authors.forename: Jamile
authors.initials: J
authors.lastname: Calil-Silveira
authors.forename: Caroline
authors.initials: C
authors.lastname: Serrano-Nascimento
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Nunes
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D027963:Chloride-Bicarbonate Antiporters / Q000235:genetics* / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D007454:Iodides / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008713:Methimazole / Q000494:pharmacology; D010472:Perchlorates / Q000494:pharmacology; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000076988:Sulfate Transporters; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000097:blood
chemical_list: D013956:Antithyroid Agents; D027963:Chloride-Bicarbonate Antiporters; D007454:Iodides; D010472:Perchlorates; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; C494615:Slc26A4 protein, rat; D000076988:Sulfate Transporters; D014284:Triiodothyronine; D003609:Dactinomycin; D008713:Methimazole; D013972:Thyrotropin; D013974:Thyroxine; C494474:perchlorate