title: Lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims N-glycan Man9GlcNAc2 to Man8GlcNAc2 isomer B in a manE gene disruptant of Aspergillus oryzae.
abstract: The gene manE, encoding a probable class I endoplasmic reticulum 1,2-α-mannosidases (ER-Man), was identified from the filamentous fungus Aspergillus oryzae due to similarity to orthologs. It removes a single mannose residue from Man(9)GlcNAc(2), generating Man(8)GlcNAc(2) isomer B. Disruption of manE caused drastic decreases in ER-Man activity in A. oryzae microsomes.
authors.affiliation: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan. akao_t@nrib.go.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akao
authors.forename: Akinori
authors.initials: A
authors.lastname: Yahara
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Osamu
authors.initials: O
authors.lastname: Akita
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D007536:Isomerism; D008351:Mannans / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D043323:alpha-Mannosidase / Q000235:genetics* / Q000378:metabolism*
chemical_list: D008351:Mannans; C475461:mannosyl(9)-N-acetylglucosamine2; D043323:alpha-Mannosidase; D008358:Mannose