title: PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory.
abstract: We have evaluated the GenoType blood culture assay (Hain Lifescience, Nehren, Germany) for the identification of bacteria in 233 positive blood cultures and assessed its suitability in the workflow of a routine microbiology laboratory. In 68/233 (29.2%) samples, the culture result could not be confirmed by the GenoType assay due to a lack of primers in the test, multiple organisms in the sample, or inconsistency with respect to the identification by culture. Although the GenoType blood culture assay gives satisfactory results for bacteria for which primers are available, there are difficulties in applying the test in the routine microbiology laboratory.
authors.affiliation: Päijät-Häme Social and Health Care Group, Clinical Microbiology, Lahti, Finland.
authors.forename: Santra
authors.initials: S
authors.lastname: Karumaa
authors.forename: Pauliina
authors.initials: P
authors.lastname: Kärpänoja
authors.forename: Hannu
authors.initials: H
authors.lastname: Sarkkinen
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D005858:Germany; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D057188:Workflow
chemical_list: D017931:DNA Primers