title: Genomic approach to study floral development genes in Rosa sp.
abstract: Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower.
authors.affiliation: Laboratoire Reproduction et Développement des Plantes, Institut Nationale de la Recherche Agronomique, Centre National de la Recherche Scientifique, Ecole Normale Supérieure, Lyon, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Dubois
authors.forename: Arnaud
authors.initials: A
authors.lastname: Remay
authors.forename: Olivier
authors.initials: O
authors.lastname: Raymond
authors.forename: Sandrine
authors.initials: S
authors.lastname: Balzergue
authors.forename: Aurélie
authors.initials: A
authors.lastname: Chauvet
authors.forename: Marion
authors.initials: M
authors.lastname: Maene
authors.forename: Yann
authors.initials: Y
authors.lastname: Pécrix
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Yang
authors.forename: Julien
authors.initials: J
authors.lastname: Jeauffre
authors.forename: Tatiana
authors.initials: T
authors.lastname: Thouroude
authors.forename: Véronique
authors.initials: V
authors.lastname: Boltz
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Martin-Magniette
authors.forename: Stéphane
authors.initials: S
authors.lastname: Janczarski
authors.forename: Fabrice
authors.initials: F
authors.lastname: Legeai
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Renou
authors.forename: Philippe
authors.initials: P
authors.lastname: Vergne
authors.forename: Manuel
authors.initials: M
authors.lastname: Le Bris
authors.forename: Fabrice
authors.initials: F
authors.lastname: Foucher
authors.forename: Mohammed
authors.initials: M
authors.lastname: Bendahmane
mesh_terms: D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D050437:Genes, Developmental / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D018519:Meristem / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D027826:Rosa / Q000235:genetics* / Q000254:growth & development*; D059467:Transcriptome / Q000235:genetics