title: Heterologous production of fungal effectors in Pichia pastoris.
abstract: In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein. This method has successfully been used for the production of a number of fungal effector proteins.
authors.affiliation: Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands. anja.kombrink@wur.nl
authors.forename: Anja
authors.initials: A
authors.lastname: Kombrink
mesh_terms: D019149:Bioreactors; D003001:Cloning, Molecular / Q000379:methods; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D007218:Industrial Microbiology / Q000379:methods*; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins