title: Antimicrobial properties, antioxidant activity and cytotoxicity of ethanol-soluble acidic components from Ganoderma atrum.
abstract: The aim of this study was to investigate the possibility of ethanol-soluble acidic components (ESAC) from Ganoderma atrum as a novel source against bacteria and oxidation in food industry. The qualitative analysis of ESAC was based on liquid chromatography-mass spectrometry (LC/MS) and eight ganoderic acids were identified in ESAC. Its total phenolic content of ESAC was 75.80±5.67 mg/g. ESAC exhibited antimicrobial activity against all of the tested bacteria (Staphylococcus aureus subsp. Aureus, Escherichia coli, Bacillus subtilis and Proteusbacillus vulgaris), and exerted antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, total reducing power and β-carotene bleaching assays. Moreover, ESAC (at concentrations of 0-100 μg/mL) did not show any cytotoxic effects in RAW264.7 murine macrophage cells. Our findings suggested that ESAC has the potential as a novel natural source for food industry, ganoderic acids and phenolic compounds may be related to its antimicrobial properties as well as antioxidant activities.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Li
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002853:Chromatography, Liquid; D000431:Ethanol / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts; D000431:Ethanol