title: Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress.
abstract: OBJECTIVE
  Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1.
  
  
  RESULTS
  Pulses of transmitted energy (fluence) reaching 1·8Jcm(-2) can kill 10(9) bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress-induced proteins, phage-related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators.
  
  
  CONCLUSIONS
  A response to a low-energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage-related proteins, probably to protect the bacterium against a new PL-induced stress.
  
  
  CONCLUSIONS
  Taken together, these results suggest that a low-energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.
authors.affiliation: LMDF-SME, Laboratoire de Microbiologie du Froid-Signaux et Micro-Environnement, EA 4312, Université de Rouen, Rouen, France.
authors.forename: S
authors.initials: S
authors.lastname: Massier
authors.forename: A
authors.initials: A
authors.lastname: Rincé
authors.forename: O
authors.initials: O
authors.lastname: Maillot
authors.forename: M G J
authors.initials: MG
authors.lastname: Feuilloley
authors.forename: N
authors.initials: N
authors.lastname: Orange
authors.forename: S
authors.initials: S
authors.lastname: Chevalier
mesh_terms: D000222:Adaptation, Physiological; D003666:Decontamination / Q000379:methods*; D008027:Light; D050296:Microbial Viability / Q000528:radiation effects; D059645:Mutation Rate; D020543:Proteome / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D013312:Stress, Physiological
chemical_list: D020543:Proteome