title: [Investigation and analysis of a common allele HLA-C*08:22 frequency in the Chinese southern Han population].
abstract: This study was purposed to investigated and analyze the allelic frequency of a common allele HLA-C*08:22 in the southern Chinese Han population. A total of 32 samples with the C*08:01:01/08:22 ambiguous results previously identified in 163 unrelated southern Chinese Han population by routine sequencing based typing (SBT) at exons 2 - 4 of HLA-C gene were subjected to HLA-C SBT at exons 5 and 6 using our in-house method. Forty C*08:01:01-positive unrelated donor/recipient pair identified before the C*08:22 allele were officially nomenclatured and released by the World Health Organization (WHO) Nomenclature Committee for Factors of HLA System, were re-sequenced at exons 2 - 6 of HLA-C gene by our in-house SBT method. The allele assignment was accomplished with the Assign 3.5 SBT software. The results showed that three samples were identified as C*08:22-positive in the 32 samples with C*08:01:01/08:22 ambiguous results, the allele frequency of C*08:22 was 0.92% in the southern Chinese Han population. Retrospective analysis indicated that 2 donor/recipient pairs previously identified as C*08:01:01-positive were actually C*08:22-positive in the 40 tested donor/recipient pairs. It is concluded that the novel C*08:22 allele is the common allele in southern Chinese Han population, it can't be considered as rare allele and is ruled out for the samples with C*08:01:01/08:22 ambiguous results.
authors.affiliation: Shenzhen Blood Center, Shenzhen, Guangdong Province, China. szbzq@sina.com
authors.forename: Zi-Qian
authors.initials: ZQ
authors.lastname: Bao
authors.forename: Da-Ming
authors.initials: DM
authors.lastname: Wang
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Deng
authors.forename: Yuan-Ping
authors.initials: YP
authors.lastname: Xu
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D005787:Gene Frequency; D005828:Genetics, Population; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA; D061349:Unrelated Donors
chemical_list: D015236:HLA-C Antigens