title: Baupain, a plant cysteine proteinase that hinders thrombin-induced human platelet aggregation.
abstract: Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP- and collagen- induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1.
authors.affiliation: Department of Biochemistry, Universidade Federal de Sao Paulo-Escola Paulista de Medicina, 04044-020 Sao Paulo, SP, Brazil.
authors.forename: Sheila S
authors.initials: SS
authors.lastname: Andrade
authors.forename: M C C
authors.initials: MC
authors.lastname: Silva
authors.forename: I E
authors.initials: IE
authors.lastname: Gouvea
authors.forename: M Y
authors.initials: MY
authors.lastname: Kondo
authors.forename: M A
authors.initials: MA
authors.lastname: Juliano
authors.forename: M U
authors.initials: MU
authors.lastname: Sampaio
authors.forename: Maria Luzia
authors.initials: ML
authors.lastname: Oliva
mesh_terms: D002452:Cell Count; D057056:Cysteine Proteases / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004151:Dipeptides / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D007700:Kinetics; D018515:Plant Leaves / Q000201:enzymology*; D010974:Platelet Aggregation / Q000187:drug effects*; D044462:Receptors, Proteinase-Activated / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism
chemical_list: D004151:Dipeptides; D005343:Fibrinolytic Agents; D005456:Fluorescent Dyes; D044462:Receptors, Proteinase-Activated; C033517:phenylalanylleucine; D057056:Cysteine Proteases; D013917:Thrombin