title: Spectroscopic and histological evaluation of wound healing progression following Low Level Laser Therapy (LLLT).
abstract: The present study focuses on the evaluation of the effect of He-Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post-wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non-illuminated controls.
authors.affiliation: Biophysics Unit, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka, India.
authors.forename: Vijendra
authors.initials: V
authors.lastname: Prabhu
authors.forename: Satish B S
authors.initials: SB
authors.lastname: Rao
authors.forename: Subhash
authors.initials: S
authors.lastname: Chandra
authors.forename: Pramod
authors.initials: P
authors.lastname: Kumar
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Rao
authors.forename: Vasudeva
authors.initials: V
authors.lastname: Guddattu
authors.forename: Kapaettu
authors.initials: K
authors.lastname: Satyamoorthy
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Mahato
mesh_terms: D000818:Animals; D001706:Biopsy; D003094:Collagen / Q000737:chemistry*; D018450:Disease Progression; D004867:Equipment Design; D005260:Female; D006097:Granulation Tissue / Q000473:pathology*; D007249:Inflammation; D007834:Lasers; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D055095:Optics and Photonics / Q000379:methods; D012867:Skin / Q000473:pathology; D013053:Spectrophotometry / Q000379:methods*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D003094:Collagen