title: Detection of fungal DNA in lysis-centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients.
abstract: We report data concerning the detection of fungal DNA directly from lysis-centrifugation blood culture to assess its value in the detection of fungaemia in 86 of the 347 patients admitted to the neonatal intensive-care unit between January 2009 and December 2010. The sensitivity and specificity of the PCR were 87.5% and 98.5%, respectively, with a positive predictive value of 93.3% and a negative predictive value of 97.1%. Detection of fungal DNA directly from blood culture Isolator 1.5 microbial tubes, without prior cultivation, is a promising approach for the rapid detection of Candida spp. in neonates with suspected candidaemia.
authors.affiliation: Department of Bio-Medical Science, University of Catania, Laboratory Analysis Unit, Catania, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Trovato
authors.forename: P
authors.initials: P
authors.lastname: Betta
authors.forename: M G
authors.initials: MG
authors.lastname: Romeo
authors.forename: S
authors.initials: S
authors.lastname: Oliveri
mesh_terms: D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D058365:Candidiasis, Invasive / Q000175:diagnosis*; D002498:Centrifugation / Q000379:methods; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D016469:Fungemia / Q000175:diagnosis; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal