title: Transformation of Botrytis cinerea by direct hyphal blasting or by wound-mediated transformation of sclerotia.
abstract: BACKGROUND
  Botrytis cinerea is a haploid necrotrophic ascomycete which is responsible for 'grey mold' disease in more than 200 plant species. Broad molecular research has been conducted on this pathogen in recent years, resulting in the sequencing of two strains, which has generated a wealth of information toward developing additional tools for molecular transcriptome, proteome and secretome investigations. Nonetheless, transformation protocols have remained a significant bottleneck for this pathogen, hindering functional analysis research in many labs.
  
  
  RESULTS
  In this study, we tested three different transformation methods for B. cinerea: electroporation, air-pressure-mediated and sclerotium-mediated transformation. We demonstrate successful transformation with three different DNA constructs using both air-pressure- and sclerotium-mediated transformation.
  
  
  CONCLUSIONS
  These transformation methods, which are fast, simple and reproducible, can expedite functional gene analysis of B. cinerea.
authors.affiliation: Postharvest Science of Fresh Produce, The Volcani Center, ARO, Israel.
authors.forename: Shahar
authors.initials: S
authors.lastname: Ish-Shalom
authors.forename: Aviva
authors.initials: A
authors.lastname: Gafni
authors.forename: Amnon
authors.initials: A
authors.lastname: Lichter
authors.forename: Maggie
authors.initials: M
authors.lastname: Levy
mesh_terms: D001709:Biotechnology / Q000379:methods*; D020171:Botrytis / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D018274:Electroporation; D018014:Gene Transfer Techniques; D025301:Hyphae / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal