title: Changes of progesterone-induced blocking factor in patients after kidney transplantation.
abstract: The prediction of graft rejection can play an important part in graft survival. Analysis of immune reactions has shown that graft rejection shares mechanisms with recurrent abortions during pregnancy. Progesterone-induced blocking factor (PIBF), a mediator of progesterone that blocks natural killer cell activity in peripheral blood, produces antiabortive effects. The aim of this study was to examine the PIBF concentration in the urine of transplanted recipients. The study included 116 white adults (70 men and 46 women) of median age 49.3 years, who had undergone kidney transplantations. The median duration after transplantation was 3.46 years. The average period between renal disease and our measurement was 12.3 years, and the median interval between graft rejection and our study was 1.75 years. Urine samples were used to measure PIBF concentrations by an enzyme-linked immunsorbent assay. PIBF urinary concentrations decreased significantly in patients who experienced ≥1 rejection episode (31.8±2.2 ng/mL) compared with those without any episode (22.7±1.2 ng/ml; P<.01). Moreover, the urinary PIBF level was significantly lower among patients who had increased creatinine and urea nitrogen levels in blood samples (P<.05 and P<.01, respectively). Decreased PIBF values in kidney transplant patients followed previous rejection episodes. A close negative correlation was observed between urinary PIBF concentrations and blood levels of creatinine and urea nitrogen. These findings suggested that PIBF detection may predict graft rejection in transplant recipients.
authors.affiliation: Department of Dermatology, Venereology and Oncodermatology, University of Pécs, Pécs, Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Fekecs
authors.forename: K
authors.initials: K
authors.lastname: Kalmár-Nagy
authors.forename: P
authors.initials: P
authors.lastname: Szakály
authors.forename: K
authors.initials: K
authors.lastname: Németh
authors.forename: M
authors.initials: M
authors.lastname: Moezzi
authors.forename: I
authors.initials: I
authors.lastname: Zapf
authors.forename: O P
authors.initials: OP
authors.lastname: Horváth
authors.forename: J
authors.initials: J
authors.lastname: Bartho-Szekeres
authors.forename: A
authors.initials: A
authors.lastname: Ferencz
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000652:urine; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006084:Graft Rejection / Q000209:etiology* / Q000652:urine; D006801:Humans; D006814:Hungary; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011257:Pregnancy Proteins / Q000652:urine*; D012372:ROC Curve; D013491:Suppressor Factors, Immunologic / Q000652:urine*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; C110058:PIBF1 protein, human; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; D003404:Creatinine