title: [Amino-terminal pro-brain natriuretic peptide and brain natriuretic peptide measurements under various detection conditions in patients with chronic heart failure].
abstract: OBJECTIVE
  To find the potential interference factors for the detection of NT-proBNP and BNP in patients with chronic heart failure.
  
  
  METHODS
  EP15-A2 issued by Clinical and Laboratory Standards Institute (CLSI) was employed to compare the precision and accuracy of commercial NT-proBNP and BNP analyzer electrochemiluminescence immunoassay system Cobas E601 and chemiluminescence system ADVIA Centaur. Moreover, NT-proBNP and BNP were detected in different time interval and in different interfered sampling conditions (haematolysis, choloplania, lipemia). NT-proBNP and BNP of 203 patients with heart failure or heart failure complicated with acute cerebral infarction were analyzed to find the deviation caused by patients' endogenous factors.
  
  
  RESULTS
  The precision and accuracy were comparable for NT-proBNP and BNP detection using Cobas E601 and ADVIA Centaur (total-CV below 2.9% and 3.5%, the deviation from definite value below 2.38% and 3.91%). The most suitable sample type for NT-proBNP and BNP detection was serum and EDTA-anticoagulant plasma. The detection results of NT-proBNP and BNP were comparable for at least 120 min post sampling and not affected by Hb (2 g/L), DB (428 µmol/L) and chyle (2000 FIU). NT-proBNP was significantly higher in heart failure patients complicated with cerebral infarction (P = 0.003) than in heart failure patients. BNP was significantly higher in heart failure grade III patients complicated with cerebral infarction (P < 0.01).
  
  
  CONCLUSIONS
  Cobas E601 and ADVIA Centaur supplied satisfactory detection of NT-proBNP and BNP in patients with chronic heart failure with strong anti-interference capacity. The diagnostic value of NT-proBNP and BNP for chronic heart failure should be analyzed objectively in the presence of complicating diseases.
authors.affiliation: Department of Laboratory Diagnosis, SUN YAT-SEN University Affiliated Zhongshan Hospital, Zhongshan 528403, China.
authors.forename: Wei-jia
authors.initials: WJ
authors.lastname: Wang
authors.forename: Xiu-ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Wen
authors.forename: Neng-liang
authors.initials: NL
authors.lastname: Ouyang
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Cui
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods; D006333:Heart Failure / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements / Q000379:methods; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods* / Q000592:standards
chemical_list: D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain