title: Identification of New Delhi metallo-β-lactamase gene (NDM-1) from a clinical isolate of  Acinetobacter junii  in China.
abstract: New Delhi metallo-β-lactamase-1 (NDM-1) is a novel type of metallo-β-lactamase (MBL) responsible for bacterial resistance to β-lactam antibiotics.  Acinetobacter junii  was previously shown to possess a MBL phenotype; however, the genes responsible for this phenotype were not identified. In this study, we reported the identification of NDM-1 gene in a clinical isolate of A. junii from a child patient in China, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides and quinolones. The cloned NDM-1 gene contained an open reading frame of 813 bp and had a nucleotide sequence 99.9% identical (812/813) to reported NDM-1 genes carried by  Acinetobacter baumannii ,  Enterococcus faecium ,  Escherichia coli , and  Klebsiella pneumoniae . Recombinant NDM-1 protein was successfully expressed in E. coli BL21, and antibiotic sensitivities of the NDM-1-producing E. coli were largely similar to the A. junii 1454 isolate. The findings of this study raise attention to the emergence and spread of NDM-1-carrying bacteria in China.
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical College, Guangzhou, People's Republic of China. zzw6248@126.com
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhou
authors.forename: Ruili
authors.initials: R
authors.lastname: Guan
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.forename: Qiulian
authors.initials: Q
authors.lastname: Deng
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Xie
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Jielin
authors.initials: J
authors.lastname: Wang
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.forename: Can
authors.initials: C
authors.lastname: Liao
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
authors.forename: Huimin
authors.initials: H
authors.lastname: Xia
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000382:microbiology; D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D011994:Recombinant Proteins / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D047090:beta-Lactams; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1