title: Genetic isolation between the Western and Eastern Pacific populations of pronghorn spiny lobster Panulirus penicillatus.
abstract: The pronghorn spiny lobster, Panulirus penicillatus, is a circumtropical species which has the widest global distribution among all the species of spiny lobster, ranging throughout the entire Indo-Pacific region. Partial nucleotide sequences of mitochondrial DNA COI (1,142-1,207 bp) and 16S rDNA (535-546 bp) regions were determined for adult and phyllosoma larval samples collected from the Eastern Pacific (EP)(Galápagos Islands and its adjacent water), Central Pacific (CP)(Hawaii and Tuamotu) and the Western Pacific (WP)(Japan, Indonesia, Fiji, New Caledonia and Australia). Phylogenetic analyses revealed two distinct large clades corresponding to the geographic origin of samples (EP and CP+WP). No haplotype was shared between the two regional samples, and average nucleotide sequence divergence (Kimura's two parameter distance) between EP and CP+WP samples was 3.8±0.5% for COI and 1.0±0.4% for 16S rDNA, both of which were much larger than those within samples. The present results indicate that the Pacific population of the pronghorn spiny lobster is subdivided into two distinct populations (Eastern Pacific and Central to Western Pacific), with no gene flow between them. Although the pronghorn spiny lobster have long-lived teleplanic larvae, the vast expanse of Pacific Ocean with no islands and no shallow substrate which is known as the East Pacific Barrier appears to have isolated these two populations for a long time (c.a. 1MY).
authors.affiliation: Stock Enhancement and Aquaculture Division, National Research Institute of Aquaculture, Yokosuka, Kanagawa, Japan. chow@affrc.go.jp
authors.forename: Seinen
authors.initials: S
authors.lastname: Chow
authors.forename: Andrew
authors.initials: A
authors.lastname: Jeffs
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Miyake
authors.forename: Kooichi
authors.initials: K
authors.lastname: Konishi
authors.forename: Makoto
authors.initials: M
authors.lastname: Okazaki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Muhamad F
authors.initials: MF
authors.lastname: Abdullah
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Imai
authors.forename: Toshie
authors.initials: T
authors.lastname: Wakabayasi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakai
mesh_terms: D000818:Animals; D020029:Base Pairing / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D007814:Larva / Q000235:genetics; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D033521:Palinuridae / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D003576:Electron Transport Complex IV