title: A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies.
abstract: The purpose of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for analysis of the deacetyl mycoepoxydiene in rat plasma. The analyte and internal standard (I.S.), benorilate, were extracted from rat plasma by precipitation protein and separated on a C(18) column using acetonitrile-0.5% formic acid as mobile phase. Detection was performed using a turbo-spray ionization source and mass spectrometric positive multi-reaction-monitoring-mode (+MRM) at an ion voltage of +4800 V. The assay was linear over the concentration range 5-5000 ng/mL with the lowest limit of quantification (LLOQ) of 5 ng/mL. The method also afforded satisfactory results in terms of the sensitivity, specificity, precision (intra- and inter-day, RSD<5.8%), accuracy, recovery as well as the stability of the analyte under various conditions. The method was successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous administration of deacetyl mycoepoxydiene 10 mg/kg.
authors.affiliation: School of Pharmaceutical Sciences of Shandong University, Shandong University, Jinan, China. may@sdu.edu.cn
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Gao
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Xu
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Xu
authors.forename: Yaojian
authors.initials: Y
authors.lastname: Huang
authors.forename: Yuemao
authors.initials: Y
authors.lastname: Shen
authors.forename: ZhaoPing
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004852:Epoxy Compounds / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D016018:Least-Squares Analysis; D057230:Limit of Detection; D008297:Male; D011753:Pyrones / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004852:Epoxy Compounds; D011753:Pyrones; C557709:deacety mycoepoxydiene