title: Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity.
abstract: Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against γ-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against γ-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 μg/ml) for radioprotection, PM1 at 25 μg/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 μg/ml), a combination of polyphenols was found most effective in preventing γ-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component.
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India.
authors.forename: Piya
authors.initials: P
authors.lastname: Paul
authors.forename: Punit
authors.initials: P
authors.lastname: Bansal
authors.forename: Pawan G
authors.initials: PG
authors.lastname: Nayak
authors.forename: Steve Thomas
authors.initials: ST
authors.lastname: Pannakal
authors.forename: K I
authors.initials: KI
authors.lastname: Priyadarsini
authors.forename: M K
authors.initials: MK
authors.lastname: Unnikrishnan
mesh_terms: D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008168:Lung / Q000166:cytology; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D027003:Urticaceae / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D059808:Polyphenols; D011837:Radiation-Protective Agents