title: Effect of ergothioneine on acute lung injury and inflammation in cytokine insufflated rats.
abstract: OBJECTIVE
  The Acute Respiratory Distress Syndrome (ARDS), the most severe form of Acute Lung Injury (ALI), is a highly-fatal, diffuse non-cardiogenic edematous lung disorder. The pathogenesis of ARDS is unknown but lung inflammation and lung oxidative stress are likely contributing factors. Since no specific pharmacologic intervention exists for ARDS, our objective was to determine the effect of treatment with ergothioneine-a safe agent with multiple anti-inflammatory and antioxidant properties on the development of lung injury and inflammation in rats insufflated with cytokines found in lung lavages of ARDS patients.
  
  
  METHODS
  Sprague-Dawley rats (3-10/group) were given 15 mg/kg or 150 mg/kg l-ergothioneine intravenously 1h before or 18 h after cytokine (IL-1 and IFNγ) insufflation. Lung injury (lavage LDH levels) and lung inflammation (lavage neutrophil numbers) were measured 24h after cytokine insufflation.
  
  
  RESULTS
  Ergothioneine pre- and post-treatment generally decreased lung injury and lung inflammation in cytokine insufflated rats.
  
  
  CONCLUSIONS
  Ergothioneine should be considered for additional testing as a potential therapy for treating and preventing ARDS.
authors.affiliation: Webb-Waring Center, University of Colorado Denver, V20, Mail Stop C322, 12850 East Montview Blvd., Aurora, CO 80045, USA. john.repine@ucdenver.edu
authors.forename: John E
authors.initials: JE
authors.lastname: Repine
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Elkins
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000008:administration & dosage* / Q000378:metabolism; D004880:Ergothioneine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011014:Pneumonia / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D016207:Cytokines; D004880:Ergothioneine