title: Continuous two stage acetone-butanol-ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313.
abstract: The objective of this study was to optimize continuous acetone-butanol-ethanol (ABE) fermentation using a two stage chemostat system integrated with liquid-liquid extraction of solvents produced in the first stage. This minimized end product inhibition by butanol and subsequently enhanced glucose utilization and solvent production in continuous cultures of Clostridium acetobutylicum B 5313. During continuous two-stage ABE fermentation, sugarcane bagasse was used as the cell holding material for the both stages and liquid-liquid extraction was performed using an oleyl alcohol and decanol mixture. An overall solvent production of 25.32g/L (acetone 5.93g/L, butanol 16.90g/L and ethanol 2.48g/L) was observed as compared to 15.98g/L in the single stage chemostat with highest solvent productivity and solvent yield of 2.5g/Lh and of 0.35g/g, respectively. Maximum glucose utilization (83.21%) at a dilution rate of 0.051/h was observed as compared to 54.38% in the single stage chemostat.
authors.affiliation: Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, POB 16100, 00076 Aalto, Finland.
authors.forename: Sandip B
authors.initials: SB
authors.lastname: Bankar
authors.forename: Shrikant A
authors.initials: SA
authors.lastname: Survase
authors.forename: Rekha S
authors.initials: RS
authors.lastname: Singhal
authors.forename: Tom
authors.initials: T
authors.lastname: Granström
mesh_terms: D000096:Acetone / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D012997:Solvents / Q000302:isolation & purification*
chemical_list: D000440:Butanols; D012997:Solvents; D000096:Acetone; D000431:Ethanol; D005947:Glucose