title: Sca-1+ cardiac stem cells mediate acute cardioprotection via paracrine factor SDF-1 following myocardial ischemia/reperfusion.
abstract: BACKGROUND
  Cardiac stem cells (CSCs) promote myocardial recovery following ischemia through their regenerative properties. However, little is known regarding the implication of paracrine action by CSCs in the setting of myocardial ischemia/reperfusion (I/R) injury although it is well documented that non-cardiac stem cells mediate cardioprotection via the production of paracrine protective factors. Here, we studied whether CSCs could initiate acute protection following global myocardial I/R via paracrine effect and what component from CSCs is critical to this protection.
  
  
  RESULTS
  A murine model of global myocardial I/R was utilized to investigate paracrine effect of Sca-1+ CSCs on cardiac function. Intracoronary delivery of CSCs or CSC conditioned medium (CSC CM) prior to ischemia significantly improved myocardial function following I/R. siRNA targeting of VEGF in CSCs did not affect CSC-preserved myocardial function in response to I/R injury. However, differentiation of CSCs to cardiomyocytes (DCSCs) abolished this protection. Through direct comparison of the protein expression profiles of CSCs and DCSCs, SDF-1 was identified as one of the dominant paracrine factors secreted by CSCs. Blockade of the SDF-1 receptor by AMD3100 or downregulated SDF-1 expression in CSCs by specific SDF-1 siRNA dramatically impaired CSC-induced improvement in cardiac function and increased myocardial damage following I/R. Of note, CSC treatment increased myocardial STAT3 activation after I/R, whereas downregulation of SDF-1 action by blockade of the SDF-1 receptor or SDF-1 siRNA transfection abolished CSC-induced STAT3 activation. In addition, inhibition of STAT3 activation attenuated CSC-mediated cardioprotection following I/R. Finally, post-ischemic infusion of CSC CM was shown to significantly protect I/R-caused myocardial dysfunction.
  
  
  CONCLUSIONS
  This study suggests that CSCs acutely improve post-ischemic myocardial function through paracrine factor SDF-1 and up-regulated myocardial STAT3 activation.
authors.affiliation: Department of Surgery, Indiana University School of Medicine, Indianapolis, Indiana, United States of America.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Huang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Gu
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Mariuxi C
authors.initials: MC
authors.lastname: Manukyan
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Poynter
authors.forename: Meijing
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006334:Heart Function Tests / Q000187:drug effects; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000473:pathology*; D019899:Paracrine Communication / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; D002316:Cardiotonic Agents; D054377:Chemokine CXCL12; D017077:Culture Media, Conditioned; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D042461:Vascular Endothelial Growth Factor A; D051057:Proto-Oncogene Proteins c-akt