title: Acridine orange inhibits pulmonary metastasis of mouse osteosarcoma.
abstract: Although the survival of patients with osteosarcoma has improved following development of chemotherapy and surgery, the presence of pulmonary metastases indicate a poor prognosis. We developed photodynamic and radiodynamic therapies with acridine orange (AO-PDT and AO-RDT) for minimally invasive surgery to treat musculoskeletal sarcomas and reported a good clinical outcome of local control and limb function. We investigated the effect of AO-PDT using flash-wave light (FWL) on pulmonary metastasis of mouse osteosarcoma. In in vitro and in vivo studies, AO alone and AO-PDT significantly inhibited cell invasion and the growth of pulmonary metastases from primary mouse osteosarcoma. AO may have a specific metastasis-inhibitory effect, different from the effect of AO-PDT. The fluorovisualization effect on pulmonary metastases following intravenous AO administration showed that pulmonary metastases localized on the lung surface were recognized as brilliant green lesions. In conclusion, AO-PDT using FWL inhibited cell invasion and pulmonary metastases in mouse osteosarcoma; therefore, this treatment modality might be applicable for treating pulmonary metastasis from malignant musculoskeletal tumors in humans.
authors.affiliation: Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Tsu City, Mie, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Satonaka
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Kusuzaki
authors.forename: Koji
authors.initials: K
authors.lastname: Akeda
authors.forename: Masaya
authors.initials: M
authors.lastname: Tsujii
authors.forename: Takahiro
authors.initials: T
authors.lastname: Iino
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uemura
authors.forename: Takao
authors.initials: T
authors.lastname: Matsubara
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Asanuma
authors.forename: Akihiko
authors.initials: A
authors.lastname: Matsumine
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sudo
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010777:Photochemistry / Q000379:methods; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange