title: The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication.
abstract: BACKGROUND
  During the process that AIV infect hosts, the NS1 protein can act on hosts, change corresponding signal pathways, promote the translation of virus proteins and result in virus replication.
  
  
  RESULTS
  In our study, we found that PARP domain and Glu-rich region of PARP10 interacted with NS1, and the presence of NS1 could induce PARP10 migrate from cytoplasm to nucleus. NS1 high expression could reduce the endogenous PARP10 expression. Cell cycle analysis showed that with inhibited PARP10 expression, NS1 could induce cell arrest in G2-M stage, and the percentage of cells in G2-M stage rise from the previous 10%-45%, consistent with the cell proliferation result. Plague forming unit measurement showed that inhibited PARP10 expression could help virus replication.
  
  
  CONCLUSIONS
  In a word, our results showed that NS1 acts on host cells and PARP10 plays a regulating role in virus replication.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, People's Republic of China.
authors.forename: Mengbin
authors.initials: M
authors.lastname: Yu
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhang
authors.forename: Yutao
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Yang
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhao
authors.forename: Long
authors.initials: L
authors.lastname: Xu
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhou
authors.forename: Peitang
authors.initials: P
authors.lastname: Huang
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D041681:NIH 3T3 Cells; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: C085820:INS1 protein, influenza virus; D011518:Proto-Oncogene Proteins; D017361:Viral Nonstructural Proteins; C499627:PARP10 protein, human; D011065:Poly(ADP-ribose) Polymerases