title: Changes in beta-2 adrenergic receptor and AMP-activated protein kinase alpha-2 subunit in the rat vestibular nerve after labyrinthectomy.
abstract: In the present study, to elucidate the role of vestibular ganglion (VG) after the unilateral labyrinthine damage, we examined quantitative changes in mRNA expression of beta-adrenergic receptors (bARs) and AMP-activated protein kinase alpha catalytic subunits (aAMPKs) in VG after unilateral labyrinthectomy (UL) in rats. Using the real-time PCR method, beta2 AR mRNA expression in bilateral VG and AMPK alpha2 mRNA expression in the ipsilateral VG were significantly up-regulated with the maximum increase at the postoperative 7 day and 1 day, respectively. The up-regulation of beta2 AR in bilateral VG was long-lasting until 28 days after UL and that of AMPK alpha2 in the ipsilateral VG was just transient within 7 days after UL. These mRNA changes were supported by immunohistochemical data. According to previous reports, both of bARs and aAMPKs could regulate mitochondrial uncoupling protein (UCP) mRNA expression in several kinds of tissues and therefore might have thermogenic neurotransmission and antioxidant neuroprotective roles in neuronal tissues. UL requires not only long-lasting response of VG for central vestibular neuro-plasticity around 2-4 weeks but rapid response of VG against apoptosis of peripheral vestibular epithelia-neuronal synapses. The present findings suggest that beta2 AR in bilateral VG and AMPK alpha2 in the ipsilateral VG might play important signaling roles after the unilateral labyrinthine damage.
authors.affiliation: Department of Otolaryngology, Osaka University, School of Medicine, Japan. tkitahara@ent.med.osaka-u.ac.jp
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kitahara
authors.forename: Arata
authors.initials: A
authors.lastname: Horii
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Uno
authors.forename: Takao
authors.initials: T
authors.lastname: Imai
authors.forename: Suzuyo
authors.initials: S
authors.lastname: Okazaki
authors.forename: Takefumi
authors.initials: T
authors.lastname: Kamakura
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Takimoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Inohara
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D007758:Ear, Inner / Q000601:surgery*; D008297:Male; D013506:Otologic Surgical Procedures; D051381:Rats; D017207:Rats, Sprague-Dawley; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D014725:Vestibular Nerve / Q000378:metabolism*
chemical_list: D018343:Receptors, Adrenergic, beta-2; D055372:AMP-Activated Protein Kinases