title: Triterpene acids from rose hip powder inhibit self-antigen- and LPS-induced cytokine production and CD4⁺ T-cell proliferation in human mononuclear cell cultures.
abstract: A triterpene acid mixture consisting of oleanolic, ursolic and betulinic acid isolated from a standardized rose hip powder (Rosa canina L.) has been shown to inhibit interleukin (IL)-6 release from Mono Mac 6 cells. The present study examined the effects of the triterpene acid mixture on the cytokine production and proliferation of CD4⁺ T cells and CD19⁺ B cells induced by a self-antigen, human thyroglobulin and by lipopolysaccharide in cultures of normal mononuclear cells. The triterpene acid mixture inhibited the production of tumor necrosis factor-α and IL-6 with estimated IC₅₀ values in the range 35-56 µg/mL, the Th1 cytokines interferon-γ and IL-2 (IC₅₀ values 10-20 µg/mL) and the antiinflammatory cytokine IL-10 (IC₅₀ values 18-21 µg/mL). Moreover, the mixture also inhibited CD4⁺ T-cell and CD19⁺ B-cell proliferation (IC₅₀ value 22 and 12 µg/mL, respectively). Together, these data demonstrate that oleanolic, ursolic and betulinic acid are active immunomodulatory constituents of the standardized rose hip powder. However, since the estimated IC₅₀ values are in the µg/mL range, it is questionable whether the content of the triterpene acids in the standardized rose hip powder, alone, can explain the reported clinical effects.
authors.affiliation: Department of Rheumatology, Institute for Inflammation Research, Copenhagen University Hospital, Copenhagen, Denmark. las@farma.ku.dk
authors.forename: Lasse
authors.initials: L
authors.lastname: Saaby
authors.forename: Claus Henrik
authors.initials: CH
authors.lastname: Nielsen
mesh_terms: D000143:Acids / Q000494:pharmacology; D001324:Autoantigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D016753:Interleukin-10 / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D009828:Oleanolic Acid / Q000276:immunology* / Q000494:pharmacology; D053978:Pentacyclic Triterpenes; D028321:Plant Preparations / Q000276:immunology / Q000494:pharmacology; D027826:Rosa / Q000737:chemistry*; D013954:Thyroglobulin / Q000276:immunology; D014315:Triterpenes / Q000276:immunology / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D000143:Acids; D001324:Autoantigens; D007155:Immunologic Factors; D016753:Interleukin-10; D008070:Lipopolysaccharides; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D028321:Plant Preparations; D013954:Thyroglobulin; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D000094062:Betulinic Acid; D000097245:Ursolic Acid; C508609:IL10 protein, human