title: Increasing surface enhanced Raman spectroscopy effect of RNA and DNA components by changing the pH of silver colloidal suspensions.
abstract: This work focused on establishing the parameters for enhancing the Raman signals of DNA and RNA constituents: nitrogenous bases, nucleosides and nucleotides, using metallic nanoparticles as surface enhanced Raman scattering substrates. Silver nanospheres were synthesized using sodium borohydride as a reducing agent and sodium citrate as a capping agent. The prepared nanoparticles had a surface plasmon band at ∼384nm and an average size of 12±3nm. The nanoparticles' surface charge was manipulated by changing the pH of the Ag colloidal suspensions in the range of 1-13. Low concentrations as 0.7μM were detected under the experimental conditions. The optimum pH values were: 7 for adenine, 9 for AMP, 5 for adenosine, 7 for dAMP and 11 for deoxyadenosine.
authors.affiliation: Center for Sensor Development/Chemical Imaging Center/ALERT DHS-COE, Department of Chemistry, University of Puerto Rico-Mayaguez, P.O. Box 9019, Mayaguez, PR 00681, Puerto Rico. olivaprimera@yahoo.es
authors.forename: Oliva M
authors.initials: OM
authors.lastname: Primera-Pedrozo
authors.forename: Gabriela Del Mar
authors.initials: Gdel M
authors.lastname: Rodríguez
authors.forename: Jorge
authors.initials: J
authors.lastname: Castellanos
authors.forename: Hilsamar
authors.initials: H
authors.lastname: Felix-Rivera
authors.forename: Oscar
authors.initials: O
authors.lastname: Resto
authors.forename: Samuel P
authors.initials: SP
authors.lastname: Hernández-Rivera
mesh_terms: D003102:Colloids / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012313:RNA / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D003102:Colloids; D012834:Silver; D012313:RNA; D004247:DNA