title: Histone H3 lysine 14 acetylation is required for activation of a DNA damage checkpoint in fission yeast.
abstract: Histone lysine acetylation has emerged as a key regulator of genome organization. However, with a few exceptions, the contribution of each acetylated lysine to cellular functions is not well understood because of the limited specificity of most histone acetyltransferases and histone deacetylases. Here we show that the Mst2 complex in Schizosaccharomyces pombe is a highly specific H3 lysine 14 (H3K14) acetyltransferase that functions together with Gcn5 to regulate global levels of H3K14 acetylation (H3K14ac). By analyzing the effect of H3K14ac loss through both enzymatic inactivation and histone mutations, we found that H3K14ac is critical for DNA damage checkpoint activation by directly regulating the compaction of chromatin and by recruiting chromatin remodeling protein complex RSC.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Scott P
authors.initials: SP
authors.lastname: Kallgren
authors.forename: Bharat D
authors.initials: BD
authors.lastname: Reddy
authors.forename: Karen
authors.initials: K
authors.lastname: Kuntz
authors.forename: Luis
authors.initials: L
authors.lastname: López-Maury
authors.forename: James
authors.initials: J
authors.lastname: Thompson
authors.forename: Stephen
authors.initials: S
authors.lastname: Watt
authors.forename: Chun
authors.initials: C
authors.lastname: Ma
authors.forename: Haitong
authors.initials: H
authors.lastname: Hou
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: O'Connell
authors.forename: Songtao
authors.initials: S
authors.lastname: Jia
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D002843:Chromatin; D004271:DNA, Fungal; D006657:Histones; D029702:Schizosaccharomyces pombe Proteins; C501322:Gcn5 protein, S pombe; D000099224:p300-CBP-Associated Factor