title: HPV 9G DNA chip: 100% clinical sensitivity and specificity.
abstract: We describe a novel HPV 9G DNA chip test for the accurate and reliable genotyping of human papillomavirus (HPV). The HPV 9G DNA chip test established its efficiency in terms of a signal-to-background ratio (SBR) of 200, which is 50 times superior to commercial HPV DNA chips, and 100% target-specific hybridization at 25°C. We compared the genotyping results for the 439 clinical samples by the HPV 9G DNA chip test with the sequencing results for the MY11/GP6+ (M2) primer set-mediated PCR products. The discrimination of HPV genotypes in the 151 HPV-positive clinical samples by the HPV 9G DNA chip test were 100% identical with the sequencing analysis. The clinical sensitivities of HPV genotyping by the HPV 9G DNA chip test and a commercial HPV DNA chip test were 100% and 88%, respectively. However, the clinical specificities of HPV genotyping by the HPV 9G DNA chip test and the commercial HPV DNA chip test were 100% and 94%, respectively. The 100% clinical sensitivity and specificity of the HPV 9G DNA chip test make it a promising diagnostic tool for HPV genotyping.
authors.affiliation: Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, South Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: An
authors.forename: Keum-Soo
authors.initials: KS
authors.lastname: Song
authors.forename: Satish Balasaheb
authors.initials: SB
authors.lastname: Nimse
authors.forename: Junghoon
authors.initials: J
authors.lastname: Kim
authors.forename: Van-Thuan
authors.initials: VT
authors.lastname: Nguyen
authors.forename: Van-Thao
authors.initials: VT
authors.lastname: Ta
authors.forename: Danishmalik Rafiq
authors.initials: DR
authors.lastname: Sayyed
authors.forename: Taisun
authors.initials: T
authors.lastname: Kim
mesh_terms: D005260:Female; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*