title: Structure and properties of the exopolysaccharides produced by Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014.
abstract: This paper presents a study on the purification, primary structure, and rheological properties of exopolysaccharides isolated from cultures of Pseudomonas mutabilis T6 and P. mutabilis ATCC 31014. Both polymers are exopolysaccharides of D-mannose. The mannan isolated from P. mutabilis T6 contains on average about 5% of residual β-D-glucose, in contrast to the mannan from P. mutabilis ATCC 31014, which contained only trace amounts of residual β-D-glucose (less than 1%). Based on the (13)C NMR spectra, all of the remaining carbohydrates in the exopolysaccharides occur in the form of pyranose rings. All of the mannose residues have the α configuration at the anomeric carbon atom while the glucose adopts the β configuration. The reaction of both polysaccharide hydrolysates with an optically active alcohol indicates that all of the sugar residues have the D configuration. We found that the main chain of the exopolysaccharide is composed of mannose residues connected through α-(1→6) linkages, of which a large number are substituted on O2 with D-mannose and the remaining are substituted with di- to pentasaccharide fragments. The rheological properties of the exopolysaccharide isolated from P. mutabilis T6 show that its viscosity is over 30 times greater than that of P. mutabilis ATCC 31014.
authors.affiliation: Faculty of Chemistry, University of Gdańsk, Gdańsk Sobieskiego 18, 80-952 Gdańsk, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Kuźma
authors.forename: Beatrice
authors.initials: B
authors.lastname: Clack
authors.forename: Jennifer
authors.initials: J
authors.lastname: Edwards
authors.forename: Robert
authors.initials: R
authors.lastname: Tylingo
authors.forename: Justyna
authors.initials: J
authors.lastname: Samaszko
authors.forename: Janusz
authors.initials: J
authors.lastname: Madaj
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D011549:Pseudomonas / Q000737:chemistry*; D012212:Rheology; D021241:Spectrometry, Mass, Electrospray Ionization; D014783:Viscosity
chemical_list: D008351:Mannans; D011135:Polysaccharides, Bacterial; D005947:Glucose