title: The effects of pneumoperitoneum and controlled ventilation on peritoneal lymphatic bacterial clearance: experimental results in rats.
abstract: OBJECTIVE
  To evaluate the effect of pneumoperitoneum, both alone and in combination with controlled ventilation, on peritoneal lymphatic bacterial clearance using a rat bacterial peritonitis model.
  
  
  METHODS
  A total of 69 male Wistar rats were intraperitoneally inoculated with an Escherichia coli solution (10(9) colony-forming units (cfu)/mL) and divided into three groups of 23 animals each: A (control group), B (pneumoperitoneum under 5 mmHg of constant pressure), and C (endotracheal intubation, controlled ventilation, and pneumoperitoneum as in Group B). The animals were sacrificed after 30 min under these conditions, and blood, mediastinal ganglia, lungs, peritoneum, liver, and spleen cultures were performed.
  
  
  RESULTS
  Statistical analyses comparing the number of cfu/sample in each of the cultures showed that no differences existed between the three groups.
  
  
  CONCLUSIONS
  Based on our results, we concluded that pneumoperitoneum, either alone or in association with mechanical ventilation, did not modify the bacterial clearance through the diaphragmatic lymphatic system of the peritoneal cavity.
authors.affiliation: Departamento de Cirurgia da Santa Casa de Misericórdia de São Paulo, Brazil. armandangelo@uol.com.br
authors.forename: Armando Angelo
authors.initials: AA
authors.lastname: Casaroli
authors.forename: Lycia M J
authors.initials: LM
authors.lastname: Mimica
authors.forename: Belchor
authors.initials: B
authors.lastname: Fontes
authors.forename: Samir
authors.initials: S
authors.lastname: Rasslan
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology; D015169:Colony Count, Microbial; D003964:Diaphragm / Q000382:microbiology*; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000601:surgery; D008208:Lymphatic System / Q000382:microbiology*; D008297:Male; D010529:Peritoneal Cavity / Q000382:microbiology*; D010538:Peritonitis / Q000382:microbiology* / Q000601:surgery; D011028:Pneumoperitoneum, Artificial / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012121:Respiration, Artificial