title: Wound healing by a 3.2 kDa recombinant polypeptide from velvet antler of Cervus nippon Temminck.
abstract: Velvet antler (VA) is used in traditional Chinese medicine to treat a wide range of ailments including the enhancement of wound healing. A 3.2 kDa recombinant polypeptide of VA from sika deer was purified and compared to native polypeptides stimulation growth of NIH3T3 cells. Both stimulated growth in a dose-dependent manner (10-100 μg/ml). To study its wound healing properties, burn-wounded rats were topically administered with recombinant VA polypeptide or native polypeptide. Rats treated with 0.05 and 0.1% (w/w) polypeptides exhibited significant wound healing. As the yield of recombinant polypeptide was 40-fold higher than that of the native polypeptide, it may therefore be a useful biopharmaceutical.
authors.affiliation: College of Food Science, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 110866, China.
authors.forename: Enhui
authors.initials: E
authors.lastname: Zha
authors.forename: Shenyang
authors.initials: S
authors.lastname: Gao
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Pi
authors.forename: Xingxia
authors.initials: X
authors.lastname: Li
authors.forename: Yutain
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiqing
authors.initials: X
authors.lastname: Yue
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002056:Burns / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006133:Growth Substances / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D012418:Ruminants; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy / Q000473:pathology
chemical_list: D001688:Biological Products; D006133:Growth Substances; D010455:Peptides