title: Detection and identification of the atypical bovine pestiviruses in commercial foetal bovine serum batches.
abstract: The recently emerging atypical bovine pestiviruses have been detected in commercial foetal bovine serum (FBS) of mainly South American origin so far. It is unclear how widely the viruses are presented in commercial FBS of different geographic origins. To further investigate the possible pestivirus contamination of commercially available FBS batches, 33 batches of FBS were obtained from ten suppliers and analysed in this study for the presence of both the recognised and the atypical bovine pestiviruses. All 33 batches of FBS were positive by real-time RT-PCR assays for at least one species of bovine pestiviruses. According to the certificate of analysis that the suppliers claimed for each batch of FBS, BVDV-1 was detected in all 11 countries and BVDV-2 was detected exclusively in the America Continent. The atypical pestiviruses were detected in 13 batches claimed to originate from five countries. Analysis of partial 5'UTR sequences showed a high similarity among these atypical bovine pestiviruses. This study has demonstrated, for the first time that commercial FBS batches of different geographic origins are contaminated not only with the recognised species BVDV-1 and BVDV-2, but also with the emerging atypical bovine pestiviruses.
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xia
authors.forename: Balaje
authors.initials: B
authors.lastname: Vijayaraghavan
authors.forename: Sándor
authors.initials: S
authors.lastname: Belák
authors.forename: Lihong
authors.initials: L
authors.lastname: Liu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D005333:Fetus / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D044967:Serum / Q000821:virology*
chemical_list: D020121:5' Untranslated Regions