title: Nonplanar peptide bonds in proteins are common and conserved but not biased toward active sites.
abstract: The planarity of peptide bonds is an assumption that underlies decades of theoretical modeling of proteins. Peptide bonds strongly deviating from planarity are considered very rare features of protein structure that occur for functional reasons. Here, empirical analyses of atomic-resolution protein structures reveal that trans peptide groups can vary by more than 25° from planarity and that the true extent of nonplanarity is underestimated even in 1.2 Å resolution structures. Analyses as a function of the ϕ,ψ-backbone dihedral angles show that the expected value deviates by ± 8° from planar as a systematic function of conformation, but that the large majority of variation in planarity depends on tertiary effects. Furthermore, we show that those peptide bonds in proteins that are most nonplanar, deviating by over 20° from planarity, are not strongly associated with active sites. Instead, highly nonplanar peptides are simply integral components of protein structure related to local and tertiary structural features that tend to be conserved among homologs. To account for the systematic ϕ,ψ-dependent component of nonplanarity, we present a conformation-dependent library that can be used in crystallographic refinement and predictive protein modeling.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, 2011 Agriculture and Life Sciences Building, Corvallis, OR 97331, USA.
authors.forename: Donald S
authors.initials: DS
authors.lastname: Berkholz
authors.forename: Camden M
authors.initials: CM
authors.lastname: Driggers
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Shapovalov
authors.forename: Roland L
authors.initials: RL
authors.lastname: Dunbrack
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Karplus
mesh_terms: D003461:Crystallography / Q000379:methods; D008958:Models, Molecular; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019151:Peptide Library; D010455:Peptides; D011506:Proteins