title: Association between the rs2910164 polymorphism in pre-mir-146a and oral carcinoma progression.
abstract: MicroRNAs are short non-coding RNAs that regulate gene expression by RNA interference. Oral squamous cell carcinoma (OSCC) is a prevalent malignancy worldwide. miR-146a has been reported to regulate Toll-like receptors and cytokine signaling, which are both crucial for inflammation and oncogenesis. This study identifies that areca nut extract, TNFα and TGFβ up-regulates miR-146a in OSCC cells. The increased expression of miR-146a enhanced the oncogenicity of OSCC cells. In addition, a G to C polymorphism (rs2910164), which is located in the pre-miR-146a and has been associated with functional alterations in miR-146a, was significantly more prevalent among OSCC patients having more advanced nodal involvement. Our analysis also suggested a higher miR-146a expression in OSCC tissues of patients carrying C polymorphism. The present study concluded a higher prevalence of the pre-mir-146a C-variant was associated with OSCC progression in patients with this disease.
authors.affiliation: Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Pei-Shi
authors.initials: PS
authors.lastname: Hung
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Chang
authors.forename: Shou-Yen
authors.initials: SY
authors.lastname: Kao
authors.forename: Ting-Hui
authors.initials: TH
authors.lastname: Chu
authors.forename: Chung-Ji
authors.initials: CJ
authors.lastname: Liu
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Lin
mesh_terms: D001114:Areca / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism / Q000494:pharmacology; D009754:Nuts / Q000737:chemistry; D028321:Plant Preparations / Q000494:pharmacology; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D028321:Plant Preparations; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha