title: "In vitro" capacitation and subsequent acrosome reaction are related to changes in the expression and location of midpiece actin and mitofusin-2 in boar spermatozoa.
abstract: The induction of "in vitro" capacitation (IVC) and subsequent, progesterone-induced "in vitro" acrosome reaction (IVAR) was concomitant with an increase in actin polymerization, also showing an increase in actin presence at the apical area of the midpiece. The presence of mitofusin-2, a protein involved in the regulation of the coordinated mitochondrial function, expanded from midpiece to the principal piece after IVC and IVAR. All of these results indicate that the increase of boar sperm mitochondrial activity during IVC and the first minutes of IVAR is concomitant with changes in the expression and location of both actin and mitofusin-2. Our results suggest that both actin and mitofusin-2 play important roles in the modulation of boar sperm mitochondrial function, both by originating changes in the protein membrane environment and by changes in the mitochondrial structure itself.
authors.affiliation: Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Ramió-Lluch
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Novell
authors.forename: A
authors.initials: A
authors.lastname: Peña
authors.forename: D
authors.initials: D
authors.lastname: Bucci
authors.forename: T
authors.initials: T
authors.lastname: Rigau
authors.forename: J E
authors.initials: JE
authors.lastname: Rodríguez-Gil
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology*; D000199:Actins / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D020558:GTP Phosphohydrolases / Q000032:analysis*; D066298:In Vitro Techniques; D008297:Male; D024101:Mitochondrial Proteins / Q000032:analysis*; D011374:Progesterone / Q000494:pharmacology; D013075:Sperm Capacitation / Q000502:physiology*; D032961:Sperm Midpiece / Q000737:chemistry*; D034421:Sus scrofa
chemical_list: D000199:Actins; D024101:Mitochondrial Proteins; D011374:Progesterone; D020558:GTP Phosphohydrolases