title: Comparative proteomic analysis of hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera).
abstract: Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present.
authors.affiliation: Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, Italia.
authors.forename: V
authors.initials: V
authors.lastname: Amore
authors.forename: M A Puig
authors.initials: MA
authors.lastname: García
authors.forename: A M
authors.initials: AM
authors.lastname: Timperio
authors.forename: G
authors.initials: G
authors.lastname: Egidi
authors.forename: N
authors.initials: N
authors.lastname: Ubero-Pascal
authors.forename: R
authors.initials: R
authors.lastname: Fochetti
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D006433:Hemocyanins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D007313:Insecta / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; C041137:storage proteins, Insecta; D006433:Hemocyanins