title: Changes in amniotic fluid and umbilical cord serum proteomic profiles of foetuses with intrauterine growth retardation.
abstract: Foetal growth is a result of a complex net of processes, requiring coordination within the maternal, placental, and foetal compartments, the imbalance or lack of which may lead to intrauterine growth restriction (IUGR). IUGR is the major cause of perinatal morbidity and mortality, and is also related to enhanced morbidity and metabolic abnormalities later in life. In the present study, the protein profiles of umbilical cord serum (UCS) and amniotic fluid (AF) of ten IUGR and ten appropriate for gestational age newborns have been analysed by 2-DE, and nanoHPLC-Chip/MS technology. A total of 18 and 13 spots were found to be differentially expressed (p<0.01) in UCS and AF respectively. The unique differentially expressed proteins identified by MS/MS analysis were 14 in UCS, and 11 in AF samples. Protein gene ontology classification indicate that 21% of proteins are involved in inflammatory response, 20% in immune response, while a smaller proportion are related to transport, blood pressure, and coagulation. These results support the conclusion that the IUGR condition alters the expression of proteins involved in the coagulation process, immune mechanisms, blood pressure and iron and copper homeostasis control, offering a new insight into IUGR pathogenesis.
authors.affiliation: Dipartimento di Biotecnologie, Laboratorio di Proteomica e Spettrometria di massa, Università degli Studi di Verona, Verona, Italy. daniela.cecconi@univr.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Cecconi
authors.forename: Francesco
authors.initials: F
authors.lastname: Lonardoni
authors.forename: Donata
authors.initials: D
authors.lastname: Favretto
authors.forename: Erich
authors.initials: E
authors.lastname: Cosmi
authors.forename: Marianna
authors.initials: M
authors.lastname: Tucci
authors.forename: Silvia
authors.initials: S
authors.lastname: Visentin
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cecchetto
authors.forename: Paolo
authors.initials: P
authors.lastname: Fais
authors.forename: Guido
authors.initials: G
authors.lastname: Viel
authors.forename: Santo Davide
authors.initials: SD
authors.lastname: Ferrara
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry / Q000378:metabolism*; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005317:Fetal Growth Retardation / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins; D020543:Proteome