title: Coleus forskohlii extract induces hepatic cytochrome P450 enzymes in mice.
abstract: Coleus forskohlii root extract (CFE) is popular for use as a weight loss dietary supplement. In this study, the influence of standardized CFE containing 10% active component forskolin on the hepatic drug metabolizing system was investigated to evaluate the safety through its drug interaction potential. Male ICR mice were fed AIN93G-based diets containing 0-5% CFE or 0.05% pure forskolin for 2-3 weeks. Intake of two different sources of 0.5% CFE significantly increased the relative liver weight, total content of hepatic cytochrome P450 (CYP) and induced CYPs (especially 2B, 2C, 3A types) and glutathione S-transferase (GST) activities. CFE significantly increased mRNA expression of CYPs and GST with dose related responses. However, unlike the CFE, intake of 0.05% pure forskolin was found to be associated with only weak induction in CYP3A and GST activities with no significant increases in relative liver weight, total hepatic content or other CYPs activities. The inductions of CYPs and GST by CFE were observed at 1 week of feeding and rapidly recovered by discontinuation of CFE. These results indicated the induction potential of CFE on CYPs, and that this effect was predominantly due to other, as yet unidentified constituents, and not forskolin contained in CFE.
authors.affiliation: National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan.
authors.forename: Nantiga
authors.initials: N
authors.lastname: Virgona
authors.forename: Kaori
authors.initials: K
authors.lastname: Yokotani
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Chiba
authors.forename: Yuko
authors.initials: Y
authors.lastname: Taki
authors.forename: Shizuo
authors.initials: S
authors.lastname: Yamada
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Shinozuka
authors.forename: Masatsune
authors.initials: M
authors.lastname: Murata
authors.forename: Keizo
authors.initials: K
authors.lastname: Umegaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D031330:Coleus / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D017931:DNA Primers; D004790:Enzyme Induction; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008862:Microsomes, Liver / Q000201:enzymology*; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D010936:Plant Extracts; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase