title: A rapid protocol for  purification of total RNA for tissues collected from pigs at a slaughterhouse.
abstract: Since  RNA extraction is a crucial step in many molecular techniques, the protocols  for sample collection and RNA purification need to be adapted to optimize their  performance when samples are collected from animals at commercial facilities.  Here we provide an RNA purification protocol for animal tissues collected from  slaughterhouses. This protocol, modified from other techniques, uses TRIzol  Reagent. Sample collection was performed wearing sterile gloves and facemasks,  using sterile surgical instruments, and no longer than 8 min spent for each  sample. A 0.9% sterile sodium chloride solution was used to wash the tissue  before each sample collection. The whole process of RNA extraction was  performed under cold environment and sterile conditions. This protocol produced  good RNA yields (50 μg RNA  per 100 mg tissue), good integrity and purity (Abs(260/280) from 1.8 to 2.0),  from tissues such as liver, muscle, hypophysis, adipose tissue, and intestinal  mucosa, in less than 2 h.
authors.affiliation: Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Mexicali, México.
authors.forename: V
authors.initials: V
authors.lastname: Méndez
authors.forename: E
authors.initials: E
authors.lastname: Avelar
authors.forename: A
authors.initials: A
authors.lastname: Morales
authors.forename: M
authors.initials: M
authors.lastname: Cervantes
authors.forename: A
authors.initials: A
authors.lastname: Araiza
authors.forename: D
authors.initials: D
authors.lastname: González
mesh_terms: D000003:Abattoirs; D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D003080:Cold Temperature; D004587:Electrophoresis, Agar Gel; D006146:Guanidines / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D010902:Pituitary Gland / Q000737:chemistry; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000737:chemistry; D034421:Sus scrofa / Q000235:genetics*; D013552:Swine
chemical_list: D006146:Guanidines; D010636:Phenols; C411644:trizol; D012965:Sodium Chloride; D012313:RNA