title: [Experimental study on pulp revitalization of Beagle dog's immature permanent teeth after pulpectomy].
abstract: OBJECTIVE
  To observe the process of pulp revitalization of immature permanent teeth after pulpectomy.
  
  
  METHODS
  Thirty-two single-rooted teeth with open apices from 4 Beagle dogs aged 4 months were included in the study. The pulpal tissues of 8 front teeth with single root of each dog were removed, and a blood clot was produced to the level of the cementoenamel junction followed by a double seal of mineral trioxide aggregate and composite resin. At day 7, 14, 21 and 28, respectively after operation, the dog was sacrificed, and then longitudinal paraffin sections were made for histologic investigation.
  
  
  RESULTS
  After 7 days, about one thirth of the pulp chamber had an ingrowth of new tissue. After 14, 21, 28 days, there were more and more new tissue in the chamber. This new tissue consisted of well-organized and well-vascularized connective tissue. Hard tissue was too observed in the root canal. In some cases, the newly generated hard tissue even deposited against the canal dentinal walls.
  
  
  CONCLUSIONS
  The pulp of immature permanent teeth can revitalize after removal of the original pulp tissue under suitable conditions.
authors.affiliation: Department of Preventive Dentistry, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jian-zhen
authors.initials: JZ
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Liao
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D003238:Connective Tissue / Q000254:growth & development; D003782:Dental Pulp / Q000098:blood supply / Q000254:growth & development*; D003786:Dental Pulp Cavity / Q000098:blood supply / Q000254:growth & development*; D019229:Dentition, Permanent; D004285:Dogs; D007180:Incisor / Q000098:blood supply / Q000254:growth & development*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology; D011670:Pulpectomy