title: Stable expression of FRET biosensors: a new light in cancer research.
abstract: The constituents of the oncogene signal transduction pathway are promising targets for anticancer drugs. Despite the wealth of available knowledge regarding their molecular properties, the spatiotemporal regulation of the signaling molecules remains elusive. Biosensors based on the principle of FRET have been developed to visualize the activities of the signaling molecules in living cells. However, difficulties in the development of sensitive FRET biosensors have prevented their widespread use in cancer research. The lack of cell lines constitutively expressing a FRET biosensor has also limited their use. In this review, we will introduce the principle of FRET-based biosensors, describe an optimized backbone of the FRET biosensors, techniques to express FRET biosensors stably in the cells, and discuss the future perspectives of FRET biosensors in cancer research.
authors.affiliation: Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aoki
authors.forename: Naoki
authors.initials: N
authors.lastname: Komatsu
authors.forename: Eishu
authors.initials: E
authors.lastname: Hirata
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamioka
authors.forename: Michiyuki
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005544:Forecasting; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D015398:Signal Transduction