title: Healing of fresh frozen bone allograft with or without platelet-rich plasma: a histologic and histometric study in rats.
abstract: OBJECTIVE
  This study histomorphometrically analyzed the effect of autogenous platelet-rich plasma (PRP) on healing of fresh frozen bone allograft (FFBA) in bony defects in rat calvaria.
  
  
  METHODS
  A 5mm-diameter defect was created in the calvarium of 30 rats. Animals were divided into three groups: C (defect was filled by blood clot only), FFBA (defect was filled with 0.01mL of FFBA), and FFBA/PRP (defect was filled with 0.01mL of FFBA combined with 100µL of PRP). All animals were euthanized at 30 days postoperatively. Histomorphometry and histology analyses were performed. Data were statistically analyzed (analysis of variance, Tukey, p<.05).
  
  
  RESULTS
  FFBA had a statistically smaller new bone area than groups FFBA/PRP and C. No statistically significant differences were observed between groups FFBA and FFBA/PRP with regard to remaining bone graft particle area.
  
  
  CONCLUSIONS
  It can be concluded that (1) PRP improved the incorporation of FFBA, increasing the amount of new bone formed; (2) PRP has not influenced the resorption of nonviable particles of the FFBA; and (3) presence of remaining FFBA particles might have accounted for the smaller amount of new bone observed in group FFBA when compared with control group.
authors.affiliation: Division of Integrated Clinic, Dental School of Lavras, Lavras University Center - UNILAVRAS, Lavras, Brazil. michel_messora@terra.com.br
authors.forename: Michel
authors.initials: M
authors.lastname: Messora
authors.forename: Laís
authors.initials: L
authors.lastname: Braga
authors.forename: Gabriela
authors.initials: G
authors.lastname: Oliveira
authors.forename: Luiz Fernando
authors.initials: LF
authors.lastname: Oliveira
authors.forename: Roselaine
authors.initials: R
authors.lastname: Milagres
authors.forename: Leandro
authors.initials: L
authors.lastname: Kawata
authors.forename: Flávia
authors.initials: F
authors.lastname: Furlaneto
authors.forename: Natália
authors.initials: N
authors.lastname: Pola
authors.forename: Natália
authors.initials: N
authors.lastname: Campos
authors.forename: Maria
authors.initials: M
authors.lastname: Nagata
mesh_terms: D064591:Allografts / Q000473:pathology / Q000637:transplantation*; D000818:Animals; D064592:Autografts / Q000637:transplantation; D001777:Blood Coagulation / Q000502:physiology; D001847:Bone Diseases / Q000473:pathology / Q000601:surgery*; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen; D015925:Cryopreservation / Q000379:methods; D005347:Fibroblasts / Q000473:pathology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D010006:Osteoblasts / Q000473:pathology; D010011:Osteocytes / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology
chemical_list: D003094:Collagen