title: Human papillomavirus genotyping and e6/e7 mRNA expression in greek women with intraepithelial neoplasia and squamous cell carcinoma of the vagina and vulva.
abstract: A large proportion of vaginal and vulvar squamous cell carcinomas (SCCs) and intraepithelial neoplasias (VAIN and VIN) are associated with HPV infection, mainly type 16. The purpose of this study was to identify HPV genotypes, as well as E6/E7 mRNA expression of high-risk HPVs (16, 18, 31, 33, and 45) in 56 histology samples of VAIN, VIN, vaginal, and vulvar SCCs. HPV was identified in 56% of VAIN and 50% of vaginal SCCs, 71.4% of VIN and 50% of vulvar SCCs. E6/E7 mRNA expression was found in one-third of VAIN and in all vaginal SCCs, 42.9% of VIN and 83.3% of vulvar SCCs. Our data indicated that HPV 16 was the commonest genotype identified in VAIN and VIN and the only genotype found in SCCs of the vagina and vulva. These findings may suggest, in accordance with other studies, that mRNA assay might be useful in triaging lesions with increased risk of progression to cancer.
authors.affiliation: Department of Virology, "G. Papanicolaou" Research Center of Oncology and Experimental Surgery, Regional Anticancer Oncology Hospital of Athens "St. Savvas", 171 Alexandras Avenue, 11522 Athens, Greece.
authors.forename: Elpida
authors.initials: E
authors.lastname: Tsimplaki
authors.forename: Elena
authors.initials: E
authors.lastname: Argyri
authors.forename: Lina
authors.initials: L
authors.lastname: Michala
authors.forename: Maria
authors.initials: M
authors.lastname: Kouvousi
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Apostolaki
authors.forename: George
authors.initials: G
authors.lastname: Magiakos
authors.forename: Issidora
authors.initials: I
authors.lastname: Papassideri
authors.forename: Efstathia
authors.initials: E
authors.lastname: Panotopoulou