title: Cytochrome P450 1B1 contributes to renal dysfunction and damage caused by angiotensin II in mice.
abstract: Cytochrome P450 1B1 contributes to the development of angiotensin II-induced hypertension and associated cardiovascular pathophysiology. In view of the critical role of angiotensin II in the kidney, as well as in salt and water homeostasis, and blood pressure regulation, we determined the contribution of cytochrome P450 1B1 to renal dysfunction and injury associated with angiotensin II-induced hypertension in male Cyp1b1(+/+) and Cyp1b1(-/-) mice. Angiotensin II infusion (700 ng/kg per minute) given by miniosmotic pumps for 13 and 28 days increased systolic blood pressure in Cyp1b1(+/+) mice; this increase was significantly reduced in Cyp1b1(-/-) mice. Angiotensin II increased renal Cyp1b1 activity, vascular resistance, and reactivity to vasoconstrictor agents and caused endothelial dysfunction in Cyp1b1(+/+) but not Cyp1b1(-/-) mice. Angiotensin II increased water consumption and urine output, decreased urine osmolality, increased urinary Na(+) and K(+) excretion, and caused proteinuria and albuminuria in Cyp1b1(+/+) mice that was diminished in Cyp1b1(-/-) mice. Infusion of angiotensin II for 28 but not 13 days caused renal fibrosis, tubular damage, and inflammation in Cyp1b1(+/+) mice, which was minimized in Cyp1b1(-/-) mice. Angiotensin II increased levels of 12- and 20-hydroxyeicosatetraenoic acids; reactive oxygen species; and activity of NADPH oxidase, extracellular signal-regulated kinase 1/2, p38 mitogen-activated protein kinase, and c-Src in the kidneys of Cyp1b1(+/+) but not Cyp1b1(-/-) mice. These data suggest that increased thirst, renal dysfunction, and injury and inflammation associated with angiotensin II-induced hypertension in mice depend on cytochrome P450 1B1 activity, thus indicating that cytochrome P450 1B1 could serve as a novel target for treating renal disease and hypertension.
authors.affiliation: Department of Pharmacology, College of Medicine, 874 Union Ave, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: Brett L
authors.initials: BL
authors.lastname: Jennings
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Anderson
authors.forename: Anne M
authors.initials: AM
authors.lastname: Estes
authors.forename: Fariborz A
authors.initials: FA
authors.lastname: Yaghini
authors.forename: Xiao R
authors.initials: XR
authors.lastname: Fang
authors.forename: Jason
authors.initials: J
authors.lastname: Porter
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Gonzalez
authors.forename: William B
authors.initials: WB
authors.lastname: Campbell
authors.forename: Kafait U
authors.initials: KU
authors.lastname: Malik
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D000081247:CSK Tyrosine-Protein Kinase; D065633:Cytochrome P-450 CYP1B1; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005355:Fibrosis; D006973:Hypertension / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology*; D007668:Kidney / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D019255:NADPH Oxidases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012964:Sodium / Q000652:urine; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology; D019061:src-Family Kinases
chemical_list: D017382:Reactive Oxygen Species; D000804:Angiotensin II; D012964:Sodium; D001189:Aryl Hydrocarbon Hydroxylases; C585498:Cyp1b1 protein, mouse; D065633:Cytochrome P-450 CYP1B1; D019255:NADPH Oxidases; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases; D048049:Extracellular Signal-Regulated MAP Kinases