title: Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen.
abstract: Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Ohkouchi
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Tatsugawa
authors.forename: Noboru
authors.initials: N
authors.lastname: Yoshikawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takaoka
authors.forename: Sayumi
authors.initials: S
authors.lastname: Miyauchi
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Aki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Yamashita
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Murooka
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000235:genetics / Q000276:immunology; D000818:Animals; D018926:Anti-Allergic Agents / Q000276:immunology; D052179:Antigens, Plant / Q000235:genetics / Q000276:immunology; D032485:Cryptomeria / Q000235:genetics* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007778:Lactobacillus / Q000235:genetics*; D051379:Mice; D023421:Models, Animal; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology; D011058:Pollen / Q000235:genetics / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000485:Allergens; D018926:Anti-Allergic Agents; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010940:Plant Proteins; D019444:Vaccines, DNA; D007073:Immunoglobulin E