title: Autoantibody detection by direct counting of antigen-displayed yeast cells.
abstract: In this study, we report a new immunoassay platform based on yeast surface display technology for detection of autoantibodies involved in autoimmune diseases, e.g., systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). The autoantigens of Ro52/SSA epitope and SmD were chosen to be displayed on the yeast surface with their respective antibodies as the analytes. By using magnetic beads modified with protein G, yeast cells bound with specific target antibody can be captured. The amount of analytes could be determined by counting the number of fluorescent yeast cells captured in a magnetic field. The platform showed promising results in the detection of SLE autoantibodies with high sensitivity and multiplex detection capability over the traditional approaches.
authors.affiliation: Division of Biomedical Engineering, Hong Kong University of Science and Technology, Hong Kong.
authors.forename: Danhui
authors.initials: D
authors.lastname: Cheng
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Guo
authors.forename: I-Ming
authors.initials: IM
authors.lastname: Hsing
mesh_terms: D000954:Antigens, Surface / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000276:immunology; D008280:Magnetics; D008863:Microspheres; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D015003:Yeasts
chemical_list: D000954:Antigens, Surface; D001323:Autoantibodies