title: GPR54 peptide agonists stimulate insulin secretion from murine, porcine and human islets.
abstract: This study was designed to determine the effects of 10 and 13 amino acid forms of kisspeptin on dynamic insulin secretion from mammalian islets since it is not clear from published data whether the shorter peptide is stimulatory while the longer peptide inhibits insulin release. Insulin secretion was measured by radioimmunoassay following perifusion of human, pig, rat and mouse isolated islets with kisspeptin-10 or kisspeptin-13 in the presence of 20 mM glucose. Both peptides stimulated rapid, reversible potentiation of glucose-stimulated insulin secretion from islets of all species tested. These data indicate that both kisspeptin-10 and kisspeptin-13, which is an extension of kisspeptin-10 by three amino acids, act directly at islet β-cells of various species to potentiate insulin secretion, and suggest that inhibitory effects reported in earlier studies may reflect differences in experimental protocols.
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: James E
authors.initials: JE
authors.lastname: Bowe
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Foot
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Amiel
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: Gao Cai
authors.initials: GC
authors.lastname: Huang
authors.affiliation: Department of Surgery; University of California; Irvine, CA USA.
authors.forename: Morgan W
authors.initials: MW
authors.lastname: Lamb
authors.affiliation: Department of Surgery; University of California; Irvine, CA USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lakey
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Jones
authors.affiliation: Diabetes Research Group; Division of Diabetes & Nutritional Sciences; King's College London; London, UK.
authors.forename: Shanta J
authors.initials: SJ
authors.lastname: Persaud
mesh_terms: D000818:Animals; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism*; D059648:Kisspeptins / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000819:agonists; D000074502:Receptors, Kisspeptin-1; D013552:Swine
chemical_list: D007328:Insulin; C106628:KISS1 protein, human; C118688:KISS1R protein, human; C491407:Kiss1 protein, mouse; C491406:Kiss1 protein, rat; C492248:Kiss1r protein, mouse; D059648:Kisspeptins; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1