title: Spectroscopic studies on the interaction between an anticancer drug ampelopsin and bovine serum albumin.
abstract: The interaction between bovine serum albumin (BSA) and the anticancer drug molecule ampelopsin (AMP) was investigated using fluorescence spectroscopy, circular dichroism (CD) spectra, and time-resolved spectra under simulated physiological conditions. Fluorescence data showed that the intrinsic fluorescence of BSA was strongly quenched by AMP in terms of a dynamic quenching process. Binding constants and binding sites were calculated. The thermodynamic parameters indicated that the hydrogen bonding and weak van der Waals force played a major role in the interaction. The site marker competitive experiments suggested that the binding site of AMP and BSA was probably located on site III. Based on the Förster's theory, the average binding distance between AMP and BSA was obtained (r=5.47nm). The binding of AMP and BSA leaded to conformational changes of BSA according to synchronous fluorescence spectra, CD data and mean fluorescence lifetime values.
authors.affiliation: Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Shi
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Zhenpeng
authors.initials: Z
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Xie
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D027844:Vitaceae / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; C106407:ampelopsin; D012710:Serum Albumin, Bovine