title: Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model.
abstract: We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance the anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells and tissues and exert anti-oxidative activity in a mouse model of inflammation. Inflammation was induced by topical treatment of mouse ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, and reverse transcription-polymerase chain reaction data demonstrated that silk fibroin peptide or Tat-SOD alone could suppress elevated levels of cyclooxygenase-2, interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha induced by TPA. Moreover, silk fibroin peptide significantly enhanced the anti-inflammatory activity of Tat-SOD, although it had no influence on in vitro and in vivo transduction of Tat-SOD. Silk fibroin peptide exhibited anti- inflammatory activity in a mice model of inflammation. Therefore, silk fibroin peptide alone or in combination with Tat-SOD might be used as a therapeutic agent for various inflammatory diseases.
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Hwang
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Sheen
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Heo
authors.forename: Gyojun
authors.initials: G
authors.lastname: Hwang
authors.forename: Suk Hyung
authors.initials: SH
authors.lastname: Kang
authors.forename: HaeYong
authors.initials: H
authors.lastname: Kweon
authors.forename: You-Young
authors.initials: YY
authors.lastname: Jo
authors.forename: Seok Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Kwang-Gill
authors.initials: KG
authors.lastname: Lee
authors.forename: Kye Won
authors.initials: KW
authors.lastname: Park
authors.forename: Kyu Hyung
authors.initials: KH
authors.lastname: Han
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Cho
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Choi
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012831:Bombyx / Q000737:chemistry; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D005349:Fibroins / Q000737:chemistry*; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives
chemical_list: C554292:12-O-tetradecanoylphorbol-1,3-acetate; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015696:Gene Products, tat; D010455:Peptides; D005349:Fibroins; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate