title: Actinoplanes atraurantiacus sp. nov., isolated from soil.
abstract: A Gram-positive-staining bacterium, designated Y16(T), was isolated from a soil sample from Yunnan Province, China. The isolate grew optimally at 25-30 °C, grew at pH 6.0-9.0 and could grow with 3 % NaCl. Strain Y16(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinones were MK-9(H(4)) and MK-9(H(2)). The major fatty acid methyl esters were anteiso-C(15 : 0), iso-C(15 : 0), anteiso-C(17 : 0) and C(16 : 0). These chemotaxonomic characteristics suggested that the organism belonged to the genus Actinoplanes. Strain Y16(T) shared 98.7, 98.3 and 97.9 % 16S rRNA gene sequence similarity with Actinoplanes deccanensis IFO 13994(T), A. abujensis A4029(T) and A. brasiliensis DSM 43805(T), respectively. The DNA G+C content of the isolate was 70.8 mol%. DNA-DNA relatedness between the novel isolate and the type strains of A. deccanensis, A. abujensis and A. brasiliensis was 35.2, 32.0 and 22.3 %, respectively. In addition, the pattern of phenotypic properties distinguished strain Y16(T) from its closest phylogenetic neighbours. It is therefore concluded that strain Y16(T) ( = CGMCC 4.6857(T) = JCM 17700(T)) represents a novel species of the genus Actinoplanes, for which the name Actinoplanes atraurantiacus sp. nov. is proposed.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Yabo
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.affiliation: Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Huancheng
authors.initials: H
authors.lastname: Pang
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xin
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012987:Soil; D024482:Vitamin K 2; D003960:Diaminopimelic Acid