title: Coenzyme Q10 protects against amyloid beta-induced neuronal cell death by inhibiting oxidative stress and activating the P13K pathway.
abstract: Oxidative stress plays critical roles in the pathogenic mechanisms of several neurodegenerative disorders including Alzheimer's disease (AD), thus much research effort has focused on antioxidants as potential treatment agents for AD. Coenzyme Q10 (CoQ10) is known to have powerful antioxidant effects. We investigated the neuroprotective effects of CoQ10 against Amyloid beta(25-35) (Aβ(25-35))-induced neurotoxicity in rat cortical neurons. To evaluate the neuroprotective effects of CoQ10 on Aβ(25-35)-injured neurons, primary cultured cortical neurons were treated with several concentrations of CoQ10 and/or Aβ(25-35) for 48h. CoQ10 protected neuronal cells against Aβ(25-35)-induced neurotoxicity in a concentration-dependent manner. These neuroprotective effects of CoQ10 were blocked by LY294002 (10μM), a phosphatidylinositol 3-kinase (PI3K) inhibitor. Aβ(25-35) concentration-dependent increased free radical levels in rat cortical neurons, while combined treatment with CoQ10 reduced these free radical levels in a dose-dependent manner. Meanwhile, CoQ10 treatment of Aβ(25-35)-injured primary cultured cortical neurons increased the expression levels of p85aPI3K, phosphorylated Akt, phosphorylated glycogen synthase kinase-3β, and heat shock transcription factor, which are proteins related to neuronal cell survival, and decreased the levels of cytosolic cytochrome c and cleaved caspase-3, which are associated with neuronal cell death. Together, these results suggest that the neuroprotective effects of CoQ10 on Aβ(25-35) neurotoxicity are mediated by inhibition of oxidative stress together with activation of the PI3-K/Akt pathway.
authors.affiliation: Department of Neurology, Hanyang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hojin
authors.initials: H
authors.lastname: Choi
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Park
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Koh
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Choi
authors.forename: Hyun-Jeung
authors.initials: HJ
authors.lastname: Yu
authors.forename: Jinse
authors.initials: J
authors.lastname: Park
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Kyu-Yong
authors.initials: KY
authors.lastname: Lee
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D045304:Cytochromes c / Q000378:metabolism; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D038362:Glycogen Synthase Kinase 3; D000076249:Heat Shock Transcription Factors; D020287:In Situ Nick-End Labeling / Q000379:methods; D007211:Indoles; D009474:Neurons / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*; D014157:Transcription Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D005609:Free Radicals; D000076249:Heat Shock Transcription Factors; D007211:Indoles; D010446:Peptide Fragments; D014157:Transcription Factors; D014815:Vitamins; C074636:amyloid beta-protein (25-35); D014451:Ubiquinone; C007293:DAPI; D045304:Cytochromes c; D019869:Phosphatidylinositol 3-Kinases; D038362:Glycogen Synthase Kinase 3; C024989:coenzyme Q10