title: Expression of δNp73 in hippocampus of APP/PS1 transgenic mice following GFP-BMSCs transplantation.
abstract: OBJECTIVE
  To study the effect of hippocampal bone marrow stromal cells (GFP-BMSCs) transplantation on spatial memory and DeltaNp73 expression in APP/PS1 transgenic mice.
  
  
  METHODS
  Twelve APP/PS1 transgenic mice randomly received either 10 μl GFP-BMSCs  suspension in medium (GFP-BMSCs transplantation group) or 10 μl complete medium (sham-operated group). Learning and memory function of mice in both groups were observed and tested in Morris water maze experiment at 2 weeks after surgery. Senile plaques and DeltaNp73 protein in hippocampuses  were determined by immunohistochemistry and western blot at 3 weeks after surgery, respectively.
  
  
  RESULTS
  APP/PS1 mice treated with BMSCs performed significantly better on the water maze test than those in sham-operated group (P<0·05). Immunohistochemistry showed  that GFP-BMSCs distributed uniformly and the number of Alzheimer's senile plaques reduced after transplantation. Western blot showed that quantified DeltaNp73 protein expression was significantly higher in BMSCs transplantation group when compared with sham-operated group (P<0·01).
  
  
  CONCLUSIONS
  Our results suggest that BMSCs transplatation could retard Alzheimer's disease (AD) like pathology and upregulate DeltaNp73 expression in hippocampuses of APP/PS1 transgenic mice. GFP-BMSCs transplantation will be a potential treatment for AD.
authors.affiliation: The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Wen
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Qi
authors.forename: Ya-Jing
authors.initials: YJ
authors.lastname: Ren
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Liu
authors.forename: Feng-Chao
authors.initials: FC
authors.lastname: Gong
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.forename: Sheng
authors.initials: S
authors.lastname: Bi
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000378:metabolism*; D053764:Presenilin-1 / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D016896:Treatment Outcome
chemical_list: D016564:Amyloid beta-Protein Precursor; D009687:Nuclear Proteins; D053764:Presenilin-1; C454920:delta Np73, mouse; D049452:Green Fluorescent Proteins