title: MAP1B 1-126 interacts with tubulin isoforms and induces neurite outgrowth and neuronal death of cultured cortical neurons.
abstract: Previously we reported that overexpression of MAP1B containing N-terminal 126 amino acids promoted neuronal death. Here, we identified α-, β-, and βIII-tubulins as proteins interacting with MAP1B 1-126 by two-hybrid and pull-down assays. Transfection experiments indicated that MAP1B 1-126 interacts with microtubules, but to a much lesser extent than two previously reported microtubule-binding domains. Overexpression of MAP1B 1-126 induced both neurite extension and neuronal death, suggesting that MAP1B 1-126 could be involved in neuronal degeneration and aberrant sprouting.
authors.affiliation: Gene Expression Research Group, Research Team for Geriatic Disorders, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabasiku, Tokyo 173-0015, Japan. gomif@tmig.or.jp
authors.forename: Fujiya
authors.initials: F
authors.lastname: Gomi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Uchida
mesh_terms: D000818:Animals; D019556:COS Cells; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D002522:Chlorocebus aethiops; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D014404:Tubulin / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D020033:Protein Isoforms; D014404:Tubulin; C085284:microtubule-associated protein 1B