title: Hydrogen peroxide (H₂O₂) supply significantly improves xanthan gum production mediated by Xanthomonas campestris in vitro.
abstract: To improve xanthan gum productivity, a strategy of adding hydrogen peroxide (H₂O₂) was studied. The method could intensify oxygen supply through degradation of H₂O₂ to oxygen (O₂). In shake flask testing, the xanthan gum yield reached 2.8% (improved by 39.4%) when adding 12.5 mM H₂O₂ after 24 h of fermentation. In fermentor testing, it was obvious that the oxygen conditions varied with the H₂O₂ addition time. Eventually, gum yield of 4.2% (w/w) was achieved (increased by 27.3%). Compared with the method of intense mixing and increasing the air flow rate, adding H₂O₂ to improve the dissolved oxygen concentration was more effective and much better. Moreover, addition of H₂O₂ improved the quality of xanthan gum; the pyruvate content of xanthan was 4.4% (w/w), higher than that of the control (3.2%).
authors.affiliation: Chemical Engineering Institute, Sichuan University, Chengdu 610065, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Yongkui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D019149:Bioreactors; D005285:Fermentation; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011773:Pyruvates / Q000378:metabolism; D016959:Xanthomonas campestris / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D006861:Hydrogen Peroxide; D010100:Oxygen; C002563:xanthan gum