title: Fungal plant pathogen detection in plant and soil samples using DNA macroarrays.
abstract: PCR-based DNA array technology is one of the most suitable techniques to detect and identify multiple pathogens in a single assay. Out of the different array platforms that currently exist, membrane-based DNA macroarrays are the most convenient for plant disease diagnosis because of low costs, great sensitivity, and modest equipment requirements. Here we describe a protocol for routine detection of plant pathogens using DNA macroarrays, i.e., from sampling to analysis of hybridization results. Diagnosis can be completed within 36 h after sample collection.
authors.affiliation: Laboratory for Process Microbial Ecology and Bioinspirational Management, Consortium for Industrial Microbiology and Biotechnology, Department of Microbial and Molecular Systems, K.U. Leuven Association, Lessius Mechelen, Campus De Nayer, Sint-Katelijne-Waver, Belgium. bli@scientiaterrae.org
authors.forename: B
authors.initials: B
authors.lastname: Lievens
authors.forename: A
authors.initials: A
authors.lastname: Justé
authors.forename: K A
authors.initials: KA
authors.lastname: Willems
mesh_terms: D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal