title: Intranasal immunisation with Stx2B-Tir-Stx1B-Zot protein leads to decreased shedding in goats after challenge with Escherichia coli O157:H7.
abstract: Ruminants are an important reservoir of Escherichia coli O157:H7. To reduce E coli O157:H7 excretion by these animals could play a key role in prevention and control of human infections. In the present study, the authors used 12 three-month-old goats to evaluate the efficacy of intranasal administration of the Stx2B-Tir-Stx1B-Zot protein. These goats were inoculated on days 0 and 21 and infected with 10(10) colony-forming units (cfu) of E coli O157:H7 by oral inoculation on day 36. Faecal shedding was monitored daily for two weeks. All of six goats immunised with recombinant protein elicited significant Stx2b-Tir-Stx1b-Zot-specific serum IgG antibodies, and three of them also showed production of antigen-specific IgA in faeces. The immunised goats showed much less shedding of E coli O157:H7 after challenge. These results demonstrate the potential for the use of Stx2B-Tir-Stx1B-Zot protein in mucosal vaccine formulations to prevent colonisation and shedding of E coli O157:H7 in goats.
authors.affiliation: Key Laboratory of Engineering Research of Veterinary Bio-products of Agricultural Ministry, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, National Center for Engineering Research of Veterinary Bio-products, Zhongling St 50#, Nanjing 210014, China.
authors.forename: X-H
authors.initials: XH
authors.lastname: Zhang
authors.forename: K-W
authors.initials: KW
authors.lastname: He
authors.forename: P-D
authors.initials: PD
authors.lastname: Zhao
authors.forename: Q
authors.initials: Q
authors.lastname: Ye
authors.forename: X-T
authors.initials: XT
authors.lastname: Luan
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Yu
authors.forename: L-B
authors.initials: LB
authors.lastname: Wen
authors.forename: Y-X
authors.initials: YX
authors.lastname: Ni
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: X-M
authors.initials: XM
authors.lastname: Wang
authors.forename: R-L
authors.initials: RL
authors.lastname: Guo
authors.forename: J-M
authors.initials: JM
authors.lastname: Zhou
authors.forename: A-H
authors.initials: AH
authors.lastname: Mao
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D057138:Bacterial Shedding / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000276:immunology* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000276:immunology*; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission; D006041:Goats; D006801:Humans; D008297:Male; D011897:Random Allocation; D022622:Shiga Toxin 1 / Q000276:immunology; D022641:Shiga Toxin 2 / Q000276:immunology
chemical_list: D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2