title: Detection of Giardia intestinalis assemblages A, B and D in domestic cats from Warsaw, Poland.
abstract: Giardia intestinalis is a complex species divided into 7 assemblages (A - G). Two of them (A and B) are infective for both humans and animals. In cats four assemblages can occur: A, B, D, and F Assemblages A and B infect either cats, dogs and humans, assemblage D infects cats and dogs and assemblage F only cats. The purpose of this study was to determine the prevalence and genotypes of G. intestinalis in cats from Warsaw. From November 2006 to March 2007 a hundred sixty samples of stool were collected and examined by light microscopy. G. intestinalis cysts were detected in 3.75% of samples. DNA extracted from positive samples was used as template for PCR-RFLP using Giardia specific primers and the amplicons were sequenced. A comparison of the obtained DNA sequences with the Giardia sequences in the GeneBank database revealed assemblage A in 1.25% of the investigated cats, assemblage B in 1.25% and D in 1.25%.
authors.affiliation: Institute for Medical Biology of the Polish Academy of Sciences, Łódź, Poland. djaros@cbm.pan.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Jaros
authors.forename: Wojciech
authors.initials: W
authors.lastname: Zygner
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jaros
authors.forename: Halina
authors.initials: H
authors.lastname: Wedrychowicz
mesh_terms: D000818:Animals; D002415:Cats / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D016829:Giardia lamblia / Q000302:isolation & purification*; D011044:Poland; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D016054:DNA, Protozoan