title: Multilayer DNA origami packed on hexagonal and hybrid lattices.
abstract: "Scaffolded DNA origami" has been proven to be a powerful and efficient approach to construct two-dimensional or three-dimensional objects with great complexity. Multilayer DNA origami has been demonstrated with helices packing along either honeycomb-lattice geometry or square-lattice geometry. Here we report successful folding of multilayer DNA origami with helices arranged on a close-packed hexagonal lattice. This arrangement yields a higher density of helical packing and therefore higher resolution of spatial addressing than has been shown previously. We also demonstrate hybrid multilayer DNA origami with honeycomb-lattice, square-lattice, and hexagonal-lattice packing of helices all in one design. The availability of hexagonal close-packing of helices extends our ability to build complex structures using DNA nanotechnology.
authors.affiliation: Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ke
authors.forename: Niels V
authors.initials: NV
authors.lastname: Voigt
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
authors.forename: William M
authors.initials: WM
authors.lastname: Shih
mesh_terms: D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA