title: Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam.
abstract: In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).
authors.affiliation: Department of Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bianchini
authors.forename: A
authors.initials: A
authors.lastname: Diaspro
mesh_terms: D000818:Animals; D002417:Cattle; D042783:Endothelial Cells / Q000648:ultrastructure*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011651:Pulmonary Artery / Q000648:ultrastructure