title: Promoter architecture of mouse olfactory receptor genes.
abstract: Odorous chemicals are detected by the mouse main olfactory epithelium (MOE) by about 1100 types of olfactory receptors (OR) expressed by olfactory sensory neurons (OSNs). Each mature OSN is thought to express only one allele of a single OR gene. Major impediments to understand the transcriptional control of OR gene expression are the lack of a proper characterization of OR transcription start sites (TSSs) and promoters, and of regulatory transcripts at OR loci. We have applied the nanoCAGE technology to profile the transcriptome and the active promoters in the MOE. nanoCAGE analysis revealed the map and architecture of promoters for 87.5% of the mouse OR genes, as well as the expression of many novel noncoding RNAs including antisense transcripts. We identified candidate transcription factors for OR gene expression and among them confirmed by chromatin immunoprecipitation the binding of TBP, EBF1 (OLF1), and MEF2A to OR promoters. Finally, we showed that a short genomic fragment flanking the major TSS of the OR gene Olfr160 (M72) can drive OSN-specific expression in transgenic mice.
authors.affiliation: RIKEN Yokohama Institute, Omics Science Center, Yokohama, Kanagawa, Japan.
authors.forename: Charles
authors.initials: C
authors.lastname: Plessy
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pascarella
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertin
authors.forename: Altuna
authors.initials: A
authors.lastname: Akalin
authors.forename: Claudia
authors.initials: C
authors.lastname: Carrieri
authors.forename: Anne
authors.initials: A
authors.lastname: Vassalli
authors.forename: Dejan
authors.initials: D
authors.lastname: Lazarevic
authors.forename: Jessica
authors.initials: J
authors.lastname: Severin
authors.forename: Christina
authors.initials: C
authors.lastname: Vlachouli
authors.forename: Roberto
authors.initials: R
authors.lastname: Simone
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Faulkner
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Carsten O
authors.initials: CO
authors.lastname: Daub
authors.forename: Silvia
authors.initials: S
authors.lastname: Zucchelli
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Peter
authors.initials: P
authors.lastname: Mombaerts
authors.forename: Boris
authors.initials: B
authors.lastname: Lenhard
authors.forename: Stefano
authors.initials: S
authors.lastname: Gustincich
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023061:Gene Order; D056426:Genetic Loci; D064326:MEF2 Transcription Factors; D051379:Mice; D008822:Mice, Transgenic; D018006:Myogenic Regulatory Factors / Q000378:metabolism; D009831:Olfactory Mucosa / Q000378:metabolism; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D011401:Promoter Regions, Genetic; D018035:Receptors, Odorant / Q000235:genetics*; D035181:TATA-Box Binding Protein / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D064326:MEF2 Transcription Factors; C578036:Mef2a protein, mouse; D018006:Myogenic Regulatory Factors; D018035:Receptors, Odorant; D035181:TATA-Box Binding Protein; D014157:Transcription Factors