title: Reliability of solid phase microextraction in estimating bioavailability of pyrene in soil.
abstract: Solid phase microextraction (SPME) coupled with gas chromatography was employed to estimate bioavailability of pyrene in soils with different properties of textures, organic matter contents (SOM) and aging periods. Experimental results indicated that biodegradation rates increased from 0.10 (sandy loam) to 0.15 (silty loam) microg g-1 hr1. By contrast, biodegradation rate decreased from 0.10(1.3% SOM) to 0.04 (7.6% SOM) microg g-1hr1. The amounts of pyrene biodegraded decreased 27% when SOM was modified from 1.3 to 7.6%, indicating that distributions of pyrene in soils at biodegradation end points were affected by the SOM. Sequestration as measured by sonication extraction had evidently occurred in aged soil samples. SPME measurements slightly overestimated the amount of pyrene degraded by indigenous and seeded microorganisms, in soils with the different properties (correlation coefficient, R2= 0.74). The present study demonstrates that the SPME method can not replace biodegradation tests commonly used for predicting bioremediation efficacy.
authors.affiliation: Department of Environmental Engineering, National Chung Shing University, No.250, Kuo Kuang Road, Taichung, Taiwan - 40227, ROC.
authors.forename: Hsiang-Chao
authors.initials: HC
authors.lastname: Liu
authors.forename: Ching-Shyung
authors.initials: CS
authors.lastname: Hwu
authors.forename: Jui-Min
authors.initials: JM
authors.lastname: Hung
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Lai
authors.forename: Hung-Yueh
authors.initials: HY
authors.lastname: Chang
authors.forename: Chih-Jen
authors.initials: CJ
authors.lastname: Lu
mesh_terms: D001673:Biodegradation, Environmental; D004784:Environmental Monitoring; D011721:Pyrenes / Q000737:chemistry*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013997:Time Factors
chemical_list: D011721:Pyrenes; D012987:Soil; D012989:Soil Pollutants; C030984:pyrene