title: In vitro monitoring of i-NOS concentrations with an immunosensor: the inhibitory effect of endocrine disruptors on i-NOS release.
abstract: The amperometric immunosensor has demonstrated the toxicity of endocrine disrupters (EDs) through monitoring the in vitro i-NOS concentration change, where the antibody of inducible nitric oxide synthase (i-NOS) was immobilized on the conducting polymer-gold nanoparticles composite. The performance of the sensor and the experimental parameters affecting the immunoreaction were optimized. Neuronal cells treated by EDs decreased in the in vitro i-NOS concentration. The effect of bisphenol A (BPA) on the i-NOS concentration released in the cells was investigated with different incubation times, and the interfering by nonspecific binding species present in a neuronal cell lysate was also examined. Of all the tested EDs, BPA showed the inhibitoriest effect and the minimum inhibitory concentration of BPA affecting the i-NOS concentration was 0.09 ± 0.005 μM. The result shows that monitoring of i-NOS in the neuronal cells treated by EDs will be a useful method to evaluate the toxic behavior of EDs.
authors.affiliation: Department of Chemistry and Institute of Innovative BioPhysio Sensor Technology, Pusan National University, Busan 609-735, South Korea.
authors.forename: Pranjal
authors.initials: P
authors.lastname: Chandra
authors.forename: Wei Choon Alvin
authors.initials: WC
authors.lastname: Koh
authors.forename: Hui-Bog
authors.initials: HB
authors.lastname: Noh
authors.forename: Yoon-Bo
authors.initials: YB
authors.lastname: Shim
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology; D001559:Benzhydryl Compounds; D015374:Biosensing Techniques / Q000379:methods; D002460:Cell Line; D055664:Electrochemical Techniques / Q000379:methods; D052244:Endocrine Disruptors / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*; D052247:Nitric Oxide Synthase Type II / Q000032:analysis* / Q000037:antagonists & inhibitors* / Q000276:immunology; D010636:Phenols / Q000494:pharmacology*; D000098826:Bisphenol A Compounds
chemical_list: D055910:Antibodies, Immobilized; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D006046:Gold; D052247:Nitric Oxide Synthase Type II; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds