title: Poly(ε-caprolactone)/nano fluoridated hydroxyapatite scaffolds for bone tissue engineering: in vitro degradation and biocompatibility study.
abstract: In this study, biodegradation and biocompatibility of novel poly(ε-caparolactone)/nano fluoridated hydroxyapatite (PCL-FHA) scaffolds were investigated. The FHA nanopowders were prepared via mechanical alloying method and had a chemical composition of Ca(10)(PO(4))(6)OH(2-x )F(x) (where x values were selected equal to 0.5 and 2.0). In order to fabricate PCL-FHA scaffolds, 10, 20, 30 and 40 wt% of the FHA were added to the PCL. The PCL-FHA scaffolds were produced by the solvent casting/particulate leaching using sodium chloride particles (with diameters of 300-500 μm) as the porogen. The phase structure, microstructure and morphology of the scaffolds were evaluated using X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy techniques. Porosity of the scaffolds was measured using the Archimedes' Principle. In vitro degradation of PCL-FHA scaffolds was studied by incubating the samples in phosphate buffered saline at 37°C and pH 7.4 for 30 days. Moreover, biocompatibility was evaluated by MTT assay after seeding and culture of osteoblast-like cells on the scaffolds. Results showed that the osteoblast-like cells attached to and proliferated on PCL-FHA and increasing the porosity of the scaffolds increased the cell viability. Also, degradation rate of scaffolds were increased with increasing the fluorine content in scaffolds composition.
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran. n.johari@ma.iut.ac.ir
authors.forename: N
authors.initials: N
authors.lastname: Johari
authors.forename: M H
authors.initials: MH
authors.lastname: Fathi
authors.forename: M A
authors.initials: MA
authors.lastname: Golozar
authors.forename: E
authors.initials: E
authors.lastname: Erfani
authors.forename: A
authors.initials: A
authors.lastname: Samadikuchaksaraei
mesh_terms: D001672:Biocompatible Materials; D001842:Bone and Bones / Q000737:chemistry*; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite