title: Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay.
abstract: Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficacy of sterilizing and disinfectant products, used for semi critical medical devices, against the Mycobacterium bovis BCG Moreau and Mycobacterium abscessus subsp. bolletii INCQS 00594 strains by using the Confirmative in vitro Test for Determining Tuberculocidal Activity of Disinfectants recommended by the Association of Official Analytical Chemists. These changes have caused additional costs and increased problems for importation of enrichment products at national laboratories where disinfectant efficacy assay service is performed. Middlebrook ADC Enrichment (ADC) is provided by a unique manufacturer and used in the official protocol. The aim of the present study was to evaluate an alternative in house low-cost enrichment composed of fetal bovine serum and glucose (FBSG) with ADC for performance of disinfectant efficacy assay against mycobacteria. After obtaining the growth curves for M. abscessus ATCC 19977, M. abscessus subsp. bolletii INCQS 00594, Mycobacterium chelonae ATCC 35752, and Mycobacterium fortuitum ATCC 6841 by using ADC enrichment and FBSG in Kirchners and 7H9 culture media. Through statistical analysis via the Kruskal-Wallis test on the evaluation of microorganism growth rate, it was observed that there was no inhibition of RGM growth by any of the enrichments used. These results suggest that low-cost enrichment FBSG may be used as a potential substitute of ADC for composition of media for mycobacterial growth, including in disinfectant tests.
authors.affiliation: Departamento de Microbiologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Av Brasil 4365, 21040-900 Rio de Janeiro, Brazil. bruna.sabagh@incqs.fiocruz.br
authors.forename: Bruna Peres
authors.initials: BP
authors.lastname: Sabagh
authors.forename: Aline da Silva Soares
authors.initials: Ada S
authors.lastname: Souto
authors.forename: Louise Moreira
authors.initials: LM
authors.lastname: Reis
authors.forename: Sérgio Alves da
authors.initials: SA
authors.lastname: Silva
authors.forename: Daniella Cristina Rodrigues
authors.initials: DC
authors.lastname: Pereira
authors.forename: Marta de Campos
authors.initials: Mde C
authors.lastname: Neves
authors.forename: Rodrigo Rollin
authors.initials: RR
authors.lastname: Pinheiro
authors.forename: Rafael Silva
authors.initials: RS
authors.lastname: Duarte
authors.forename: Neide Hiromi Tokumaru
authors.initials: NH
authors.lastname: Miyazaki
authors.forename: Maria Helena Simões Villas
authors.initials: MH
authors.lastname: Bôas
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D003470:Culture Media / Q000737:chemistry*; D004202:Disinfectants / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D018709:Statistics, Nonparametric
chemical_list: D003470:Culture Media; D004202:Disinfectants; D005947:Glucose; D005976:Glutaral