title: Disorder gene expression of extracellular matrix and adhesion molecules in saphenous vein conduits of diabetic patients.
abstract: Saphenous vein (SV) is the most commonly employed conduit in coronary surgery. However, the extracellular matrix (ECM) characteristics of SV in diabetic patients still remain unclear. This study was to survey the ECM gene expression profile of SV in diabetic patients. Thirty-five patients had type 2 diabetic mellitus; the non-diabetic (control) group comprised 49 patients. The expression profile of ECM genes was analysed by microarray. Tissue MMP/TIMP protein activities were evaluated by immunocytochemistry and western-blot. In this microarray, 25 genes demonstrated at least a 3-fold difference in expression. Upregulation was observed in 20 genes, while five genes appeared to be downregulated. SV exposed to DM conditions demonstrated a notable increase in MMP-2 and MMP-9 but a significant decrease in TIMP-2 and TIMP-3 in protein concentration compared with control group. This study suggests that native ECM gene expression profile of SV in diabetic patients has showed signs of the vein graft disease process before coronary surgery. Preoperative profiles of diabetic patients might provide some useful clues regarding vein graft quality and prompt adjustment in surgical strategy.
authors.affiliation: Department of Cardiac Surgery,  Zhongshan Hospital, Fudan University, Shanghai,  China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Sun
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ding
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Wang Chun
authors.initials: WC
authors.lastname: Sheng
mesh_terms: D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D001026:Coronary Artery Bypass / Q000379:methods; D003324:Coronary Artery Disease / Q000150:complications / Q000601:surgery; D003920:Diabetes Mellitus / Q000235:genetics* / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006083:Graft Occlusion, Vascular / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012501:Saphenous Vein / Q000378:metabolism / Q000637:transplantation*
chemical_list: D015815:Cell Adhesion Molecules; D012313:RNA