title: Morphine-modulated mast cell migration and proliferation during early stages of zymosan-induced peritonitis in CBA mice.
abstract: We have previously shown that supplementation of inflammation-inducing zymosan with a high dose ofmorphine inhibits peritoneal influx ofleukocytes in Swiss, C57C3H, Balb/c, and C57BL/6 strains but not in CBA mice. We have also reported that the different pattern of the response to morphine treatment might be, at least partially, due to the inter-strain differences in the peritoneal mast cell (P-MC) number (high in CBA mice versus other strains) and P-MC specific features (high sensitivity to degranulation upon morphine treatment in CBA mice). The aim of the present study was to investigate the mechanism of morphine action on P-MC in CBA mice. In particular, the effects of morphine on the proliferation and migration of P-MC in CBA mice with ongoing zymosan-induced peritonitis modulated by morphine were studied. Morphine alone acted as a strong chemoattractant for P-MC of CBA mice and this effect was opioid receptor-independent. Moreover, flow cytometric analysis showed that i.p. morphine injection induced significant proliferation of P-MC in CBA mice. Therefore, we conclude that the lack of anti-inflammatory effects of morphine during peritonitis in CBA mice might result not only from a unique sensitivity of CBA mast cells to morphine-induced degranulation but also from the fact that mast cell numbers increase at the inflammatory focus. The latter might be due to morphine-induced mast cell proliferation and/or migration.
authors.affiliation: Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, R. Ingardena 6, 30-060 Kraków, Poland. ewa.wypasek@wp.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Wypasek
authors.forename: Joanna
authors.initials: J
authors.lastname: Natorska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Stankiewicz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kolaczkowska
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D009020:Morphine / Q000494:pharmacology*; D010538:Peritonitis / Q000139:chemically induced / Q000517:prevention & control*; D015054:Zymosan / Q000009:adverse effects*
chemical_list: D009020:Morphine; D015054:Zymosan