title: The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse.
abstract: Synergy between Cellulomonas flavigena xylanase CflXyn11A and Trichoderma reesei endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12-48 h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy).
authors.affiliation: Departamento de Biotecnología y Bioingeniería, CINVESTAV, Av. Instituto Politécnico Nacional No. 2508, CP 07360, México DF, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pavón-Orozco
authors.forename: Alejandro
authors.initials: A
authors.lastname: Santiago-Hernández
authors.forename: Anna
authors.initials: A
authors.lastname: Rosengren
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Hidalgo-Lara
authors.forename: Henrik
authors.initials: H
authors.lastname: Stålbrand
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D040143:Cellulomonas / Q000201:enzymology*; D002482:Cellulose / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D004789:Enzyme Activation; D006868:Hydrolysis; D009097:Multienzyme Complexes / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D009097:Multienzyme Complexes; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases