title: p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis.
abstract: Aberrant GAPDH expression following S-nitrosoglutathione (GSNO) treatment was compared in HepG2 cells, which express functional p53, and Hep3B cells, which lack functional p53. The results of Western blotting and fluorescent immunocytochemistry revealed that nuclear translocation and accumulation of GAPDH occur in both HepG2 and Hep3B cells. This finding suggests that p53 may not be necessary for the GSNO-induced translocation of GAPDH to the nucleus during apoptotic cell death in hepatoma cells.
authors.affiliation: Department of Medical Biotechnology, Soonchunhyang University, Asan 336-600, Korea.
authors.forename: Jum-Ji
authors.initials: JJ
authors.lastname: Kim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Lee
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D009569:Nitric Oxide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases