title: Altered expression pattern of testican-1 mRNA after brain injury.
abstract: Testican, a chondroitin/heparan sulfate proteoglycan, is primarily expressed in neurons of the adult and embryonic mouse brain, suggesting its role in normal and/or proliferation and differentiation processes of neurons. However, the role of testican in injured brain remains unclear. In the present study we investigated testican-1 mRNA expression pattern after cryo-injury of the brain. In situ hybridization histochemistry revealed that testican-1 mRNA is induced in the region surrounding the necrotic tissue. Time course study of testican-1 mRNA showed the highest level of signal intensity at 7 days after the injury. To determine which cell types express testican-1 mRNA, we performed in situ hybridization histochemistry combined with immunohistochemistry of several cell markers. Testican-1 mRNA signals were detected in the proximal reactive astrocytes, whereas the distribution pattern of testican-1 mRNA positive cells was different from those of mature oligodendrocytes and activated microglia. In addition, signals for testican-1 mRNA overlapped with those of FGF-2 mRNA, showing that these molecules are coexpressed in reactive astrocytes. These results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury.
authors.affiliation: Department of Emergency and Critical Care Medicine, Yamagata University, School of Medicine, Iida-Nishi 2-2-2, Yamagata City, Yamagata 990-9585, Japan. ken@med.id.yamagata-u.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
authors.forename: Seita
authors.initials: S
authors.lastname: Hagino
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Nobuko
authors.initials: N
authors.lastname: Sato
authors.forename: Sachihiko
authors.initials: S
authors.lastname: Yokoya
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Hozumi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
authors.forename: Choichiro
authors.initials: C
authors.lastname: Tase
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D011509:Proteoglycans / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D011509:Proteoglycans; D012333:RNA, Messenger; C504370:Spock1 protein, mouse