title: Isolation of fertility-related  genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP.
abstract: To  better understand the molecular mechanisms of multiple-allele-inherited male  sterility in Chinese cabbage (Brassica rapa ssp pekinensis),  differentially expressed genes in fertile and sterile plants must be isolated.  We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile  and sterile buds of the two-type line, AB01. Sixteen high-quality sequences  were generated, 11 of which were up-regulated in fertile buds, and five of  which were up-regulated in sterile buds. Based on BLAST screening and  functional annotation, these genes have homology with genes encoding known  flower- or bud-specific proteins, metabolism-related proteins and  cell-structure proteins. In addition, the full-length cDNA sequences of the actin gene were cloned from the cabbage plants by RACE and used as an internal  standard for semi-quantitative reverse transcription-PCR. Expression of three  flower- or bud-specific differentially expressed transcript-derived fragments  in fertile and sterile buds was examined using RT-PCR; the expression patterns  of these genes were similar to the patterns observed in the cDNA-AFLP analysis.
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China. ruiqinji@yahoo.com.cn
authors.forename: R Q
authors.initials: RQ
authors.lastname: Ji
authors.forename: Q
authors.initials: Q
authors.lastname: Song
authors.forename: X F
authors.initials: XF
authors.lastname: Xin
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Feng
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D029741:Brassica rapa / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry* / Q000378:metabolism; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D059467:Transcriptome
chemical_list: D000199:Actins; D018076:DNA, Complementary; D010940:Plant Proteins