title: Sphingobacterium kyonggiense sp. nov., isolated from chloroethene-contaminated soil, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium mizutaii.
abstract: A Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile strain, designated 2-1-2(T), was isolated from perchloroethylene/trichloroethene-contaminated soil in Suwon, South Korea. A polyphasic approach was used to study the taxonomic position of strain 2-1-2(T). Strain 2-1-2(T) showed highest 16S rRNA gene sequence similarities to Sphingobacterium daejeonense TR6-04(T) (97.9%) and Sphingobacterium mizutaii ATCC 33299(T) (97.1%); sequence similarities to other Sphingobacterium species were less than 93.0%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 2-1-2(T) belonged to the clade formed by members of the genus Sphingobacterium in the family Sphingobacteriaceae. The G+C content of the genomic DNA was 36.6 mol%. Strain 2-1-2(T) showed the typical chemotaxonomic features of the genus Sphingobacterium, with the presence of a ceramide phosphorylethanolamine (CerPE-2) as the major ceramide, menaquinone 7 (MK-7) as the predominant respiratory quinone and iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1)ω7c) as the major fatty acids. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, and DNA-DNA relatedness, strain 2-1-2(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium kyonggiense sp. nov. is proposed; the type strain is 2-1-2(T) (=KEMC 2241-005(T)=JCM 16704(T)). Emended descriptions of Sphingobacterium daejeonense and Sphingobacterium mizutaii are also proposed.
authors.affiliation: Department of Bio-engineering, Division of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 433-760, Republic of Korea.
authors.forename: Hyun-Ah
authors.initials: HA
authors.lastname: Choi
authors.affiliation: Department of Bio-engineering, Division of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 433-760, Republic of Korea.
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002518:Ceramides / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013750:Tetrachloroethylene; D014241:Trichloroethylene; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D002518:Ceramides; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; D014241:Trichloroethylene; C062629:menaquinone 7; D013750:Tetrachloroethylene