title: Developmental changes and subcellular location in inhibitor of DNA binding 2 (Id2) immunoreactivity in the rat Corpus callosum.
abstract: The mechanisms underlying oligodendrocyte differentiation and myelination are still unclear, but understanding them will be critical for the development of therapies for multiple sclerosis. Inhibitor of DNA binding 2 (Id2) is a transcription factor thought to inhibit oligodendrocyte differentiation, however, it is not known whether the developmental changes and subcellular localization of Id2 are related to myelination. Therefore, we investigated the developmental changes in and the subcellular localization of Id2 immunoreactivity in the rat Corpus callosum, at post-natal developmental stages P0, P7, P14, P21, P42 and P90, by immunohistochemistry. Id2 expression increased from P0 to a peak at P42, the late stage of myelination in the Corpus callosum. Id2 immunostaining decreased slightly, but still remained high at P90. Subcellular localization of Id2 changed from presence in cytoplasm at P14 to the nuclei at P42. Moreover, Id2 was mainly co-localized with CC-1-immunopositive mature oligodendrocytes at P42. These results may be consistent with Id2 inhibitory function in oligodendrocyte differentiation, at the end of myelination or in compaction of myelin in the Corpus callosum of postnatal rat brain.
authors.affiliation: Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Xing-Shu
authors.initials: XS
authors.lastname: Chen
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Ye
authors.forename: Qi-Yan
authors.initials: QY
authors.lastname: Cai
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhong-Xiang
authors.initials: ZX
authors.lastname: Yao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003337:Corpus Callosum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051797:Inhibitor of Differentiation Protein 2 / Q000235:genetics / Q000378:metabolism*; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000502:physiology; D009836:Oligodendroglia / Q000378:metabolism / Q000502:physiology; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C497451:Id2 protein, rat; D051797:Inhibitor of Differentiation Protein 2