title: Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.
abstract: A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did the LAMP primers have binding sites for the common avian DNA viruses (reticuloendotheliosis virus, chicken anemia virus, subgroup J of the avian leukosis virus). Additionally, the assay could detect up to 10 copies of the MEQ gene in the MD viral genome, and it had 10 times higher sensitivity than the traditional PCR methods. The LAMP master mix was stable for 90 days at -20°C. Furthermore, the efficiency of LAMP for detection of serotype 1 MDV in clinical samples was comparable to those of PCR and viral isolation. The LAMP procedure is simple and does not rely on any special equipment. The detection of serotype 1 MDV by LAMP will be useful for detecting and controlling oncogenic Marek's disease.
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India. raja@tanuvas.org.in
authors.forename: Raja
authors.initials: R
authors.lastname: Angamuthu
authors.forename: Subasty
authors.initials: S
authors.lastname: Baskaran
authors.forename: Dhinakar Raj
authors.initials: DR
authors.lastname: Gopal
authors.forename: Jeyanthi
authors.initials: J
authors.lastname: Devarajan
authors.forename: Kumanan
authors.initials: K
authors.lastname: Kathaperumal
mesh_terms: D000818:Animals; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005241:Feathers / Q000821:virology*; D025922:Mardivirus / Q000302:isolation & purification*; D008380:Marek Disease / Q000175:diagnosis* / Q000821:virology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009856:Oncogene Proteins, Viral / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; C100369:Eco-Q protein, Gallid herpesvirus 2; D009856:Oncogene Proteins, Viral