title: A thermophilic cellulase complex from Phialophora sp. G5 showing high capacity in cellulose hydrolysis.
abstract: A cellulase-producing mesophilic fungal strain, named G5, was isolated from the acidic wastewater and mud of a tin mine and identified as Phialophora sp. based on the internal transcribed spacer sequence. The volumetric activities and specific activities of cellulase induced by different carbon sources (Avicel, corn cob, wheat bran and corn stover) were compared. The cellulase complex of Phialophora sp. G5 exhibited the optimal activities at 60-65 °C and pH 4.0-5.0, and had good long-term thermostability at 50 °C. Compared with the commercial cellulase (Accellerase 1500, Genencor), the enzyme under study showed 60% and 80% of the capacity to hydrolyze pure cellulose and natural cellulose, respectively. This is the first study to report that a cellulytic enzymes complex from Phialophora genus, and the superior properties of this enzyme complex make strain G5 a potential microbial source to produce cellulase for industrial applications, and the production ability could be improved by mutagenesis.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing 100081, People's Republic of China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Zhao
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Bai
authors.forename: Huoqing
authors.initials: H
authors.lastname: Huang
authors.forename: Huiying
authors.initials: H
authors.lastname: Luo
authors.forename: Huitu
authors.initials: H
authors.lastname: Zhang
authors.forename: Donghao
authors.initials: D
authors.lastname: Xu
authors.forename: Yaru
authors.initials: Y
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D021901:DNA, Intergenic / Q000235:genetics; D004043:Dietary Fiber / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology; D007700:Kinetics; D010676:Phialophora / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D014866:Waste Products; D003313:Zea mays / Q000378:metabolism
chemical_list: D021901:DNA, Intergenic; D004043:Dietary Fiber; D005656:Fungal Proteins; D014866:Waste Products; D002482:Cellulose; D002480:Cellulase