title: Depth and volume of resorption induced by osteoclasts generated in the presence of RANKL, TNF-alpha/IL-1 or LIGHT.
abstract: Rheumatoid arthritis (RA) is associated with pathological bone destruction mediated by osteoclasts. Although RANKL has been reported as a crucial factor for osteoclastogenesis, several other factors increased in RA support osteoclast formation and resorption in the absence of RANKL such as TNF-alpha and LIGHT. To date, in vitro bone resorption experiments are reported as the mean area of bone resorption per cortical or dentine slices and do not provide any information about depth and volume of resorption. The aims of this study were to assess these parameters by light microscopy and vertical scanning profilometry (VSP). Peripheral blood mononuclear cells were used as a source of osteoclast precursors and were cultured for up to 21 days in the presence of RANKL, TNF-alpha/IL-1 or LIGHT. Mean area, depth and volume of resorption were assessed by light microscopy and vertical scanning profilometry. As expected, RANKL induced large resorption pits (10,876 ± 2190μm(2)) whereas TNF-alpha/IL-1 and LIGHT generated smaller pits (respectively 1328 ± 210 and 1267 ± 173μm(2)) with no noticeable differences between these two cytokines. Depth and volume of resorption measured by VSP showed that RANKL promoted deep resorption pits resulting in large volume of resorption. Interestingly, although mean area of resorption was similar between TNF-alpha/IL-1 and LIGHT, the depth and volume of resorption of these lacunae were significantly increased by 2-fold with TNF-alpha/IL-1. These results provide evidence that although LIGHT appeared elevated in the synovial fluid of RA patients, its role in bone resorption is less than TNF-alpha/IL-1 or RANKL.
authors.affiliation: INSERM, U922 - LHEA "Remodelage osseux et Biomatériaux", IRIS-IBS, Institut de Biologie en Santé, CHU d'Angers, 49933 Angers, France. guillaume.mabilleau@univ-angers.fr
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mabilleau
authors.forename: Florence
authors.initials: F
authors.lastname: Pascaretti-Grizon
authors.forename: Michel Félix
authors.initials: MF
authors.lastname: Baslé
authors.forename: Daniel
authors.initials: D
authors.lastname: Chappard
mesh_terms: D001862:Bone Resorption / Q000473:pathology*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D053145:Cell Nucleus Size / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008954:Models, Biological; D010010:Osteoclasts / Q000187:drug effects* / Q000473:pathology*; D053245:RANK Ligand / Q000494:pharmacology*; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D053245:RANK Ligand; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14; D014409:Tumor Necrosis Factor-alpha