title: Native red electrophoresis--a new method suitable for separation of native proteins.
abstract: A new type of native electrophoresis was developed to separate and characterize proteins. In this modification of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins to enable their electrophoretic separation according to their relative molecular masses. As Ponceau Red S binds less tightly to proteins, in comparison with Coomassie Blue, it can be easily removed after the electrophoretic separation and a further investigation of protein properties is made possible (e.g. an enzyme detection or electroblotting). The tested proteins also kept their native properties (enzyme activity or aggregation state).
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University, Hlavova, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Dráb
authors.forename: Jana
authors.initials: J
authors.lastname: Kračmerová
authors.forename: Ivana
authors.initials: I
authors.lastname: Tichá
authors.forename: Eva
authors.initials: E
authors.lastname: Hanzlíková
authors.forename: Marie
authors.initials: M
authors.lastname: Tichá
authors.forename: Helena
authors.initials: H
authors.lastname: Ryšlavá
authors.forename: Veronika
authors.initials: V
authors.lastname: Doubnerová
authors.forename: Pavla
authors.initials: P
authors.lastname: Maňásková-Postlerová
authors.forename: Jiří
authors.initials: J
authors.lastname: Liberda
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; C048139:Coomassie blue; C032756:ponceau S