title: [Genotyping by REA-PFGE of Listeria monocytogenes isolated from clinical, environmental and food samples].
abstract: Listeria monocytogenes strains isolated from clinical food and environmental samples were genotyped by Restriction Enzyme Analysis with Pulsed Field Gel Electrophoresis (REA-PFGE) using ApaI and AscI enzymes according to PulseNet Europe procedure. Analysis of DNA fragments profiles obtained by AscI digestion demonstrated presence of 62 REA-PFGE profiles grouped in 2 lineages (FI, FII). Diversity of strains source among both lineages was observed. Statistical analysis showed, that strains isolated from clinical samples more frequently are included to lineage FI, then lineage FII. Non-clinical strains were more frequently included to lineage FII. Combined analysis of REA-PFGE profiles for ApaI and AscI enzymes showed 8 unique pulsotypes characteristic for two or more L. monocytogenes isolates. Moreover researched L. monocytogenes strains were analyzed by multiplex-PCR according Doumith et al methodology. PCR-group 4B was most frequent among strains isolated from clinical samples. Correlation between PCR-group and pulsotype was observed only in few cases.
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego, Państwowego Zakładu Higieny.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kołakowska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Madajczak
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D004784:Environmental Monitoring / Q000379:methods*; D005516:Food Microbiology; D060005:Genotyping Techniques; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping; D013045:Species Specificity