title: Cyanidin-3-glucoside-rich extract from Chinese bayberry fruit protects pancreatic β cells and ameliorates hyperglycemia in streptozotocin-induced diabetic mice.
abstract: Chinese bayberry fruit is a rich source of anthocyanins, especially cyanidin-3-glucoside (C3G). The present study investigated the protective effects of C3G-rich bayberry fruit extract (CRBFE) against pancreatic β cells against oxidative stress-induced injury as well as its hypoglycemic effect in diabetic mice. Bayberry extract from "Biqi" was used for both in vitro and in vivo testing because of its high C3G content and high antioxidant capacity. Pretreatment of β cells with CRBFE (containing 0.5 μmol/L C3G) prevented cell death, increased cellular viability, and decreased mitochondrial reactive oxygen species production and cell necrosis induced by 800 or 1,200 μmol/L H₂O₂. CRBFE dose-dependently up-regulated pancreatic duodenal homeobox 1 gene expression, contributing to increased insulin-like growth factor II gene transcript levels and insulin protein in INS-1 cells. In addition, administration of CRBFE (150 μg of C3G/10 g of body weight twice per day) significantly reduced blood glucose in streptozotocin-induced diabetic ICR mice and increased the glucose tolerance in an oral glucose tolerance test (P<.05). Such results indicated that CRBFE might be useful in prevention and control of diabetes mellitus and diabetes-associated complications.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, China.
authors.forename: Chong-De
authors.initials: CD
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jiu-Kai
authors.initials: JK
authors.lastname: Zhang
authors.forename: Chang-Jie
authors.initials: CJ
authors.lastname: Xu
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Wu
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Kun-Song
authors.initials: KS
authors.lastname: Chen
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002681:China; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D005638:Fruit / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006943:Hyperglycemia / Q000517:prevention & control; D007004:Hypoglycemic Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D031641:Myrica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005960:Glucosides; D018398:Homeodomain Proteins; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D015534:Trans-Activators; C462279:cyanidin-3-O-beta-glucopyranoside; C084010:pancreatic and duodenal homeobox 1 protein; D007335:Insulin-Like Growth Factor II