title: Mimotopes selected with neutralizing antibodies against multiple subtypes of influenza A.
abstract: BACKGROUND
  The mimotopes of viruses are considered as the good targets for vaccine design. We prepared mimotopes against multiple subtypes of influenza A and evaluate their immune responses in flu virus challenged Balb/c mice.
  
  
  METHODS
  The mimotopes of influenza A including pandemic H1N1, H3N2, H2N2 and H1N1 swine-origin influenza virus were screened by peptide phage display libraries, respectively. These mimotopes were engineered in one protein as multi- epitopes in Escherichia coli (E. coli) and purified. Balb/c mice were immunized using the multi-mimotopes protein and specific antibody responses were analyzed using hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). The lung inflammation level was evaluated by hematoxylin and eosin (HE).
  
  
  RESULTS
  Linear heptopeptide and dodecapeptide mimotopes were obtained for these influenza virus. The recombinant multi-mimotopes protein was a 73 kDa fusion protein. Comparing immunized infected groups with unimmunized infected subsets, significant differences were observed in the body weight loss and survival rate. The antiserum contained higher HI Ab titer against H1N1 virus and the lung inflammation level were significantly decreased in immunized infected groups.
  
  
  CONCLUSIONS
  Phage-displayed mimotopes against multiple subtypes of influenza A were accessible to the mouse immune system and triggered a humoral response to above virus.
authors.affiliation: Institute of Infectious Diseases, Beijing 302 Hospital, Beijing, China. zhongyanwei@126.com
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jiong
authors.initials: J
authors.lastname: Cai
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xing
authors.forename: Enqiang
authors.initials: E
authors.lastname: Qin
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Panyong
authors.initials: P
authors.lastname: Mao
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Dongping
authors.initials: D
authors.lastname: Xu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007114:Immunization; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D053121:Influenza A Virus, H2N2 Subtype / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D009980:Influenza A virus / Q000145:classification / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control; D019151:Peptide Library
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D007252:Influenza Vaccines; D019151:Peptide Library