title: Protective effects of Commiphora erythraea resin constituents against cellular oxidative damage.
abstract: By bioguided fractionation of the hexane extract of Commiphora erythraea resin we isolated four furanosesquiterpenoids that were tested for their protective activity against oxidative stress. Furanodienone and 1,10(15)-furanogermacra-dien-6-ones showed to be potent inhibitors of lipid peroxidation (IC(50) of -0.087 μM), being more active than the methoxylated analogues. Furthermore, using BV2 microglial cells, we found that furanodienone from C. erythraea is able to counteract LPS-induced cell death and decrease LPS-induced NO generation thus protecting microglial cells from LPS-induced cytotoxicity. Finally, docking studies were undertaken to gain insight into the possible binding mode of the isolated compounds at 5-LOX binding site.
authors.affiliation: Dipartimento di Chimica e Tecnologia del Farmaco, University of Perugia, via del Liceo, 1-06123 Perugia, Italy. marcotu@unipg.it
authors.forename: Maria Carla
authors.initials: MC
authors.lastname: Marcotullio
authors.forename: Federica
authors.initials: F
authors.lastname: Messina
authors.forename: Massimo
authors.initials: M
authors.lastname: Curini
authors.forename: Antonio
authors.initials: A
authors.lastname: Macchiarulo
authors.forename: Marco
authors.initials: M
authors.lastname: Cellanetti
authors.forename: Donata
authors.initials: D
authors.lastname: Ricci
authors.forename: Laura
authors.initials: L
authors.lastname: Giamperi
authors.forename: Anahi
authors.initials: A
authors.lastname: Bucchini
authors.forename: Alba
authors.initials: A
authors.lastname: Minelli
authors.forename: Anna Lisa
authors.initials: AL
authors.lastname: Mierla
authors.forename: Ilaria
authors.initials: I
authors.lastname: Bellezza
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D029744:Commiphora / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008958:Models, Molecular; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D012116:Resins, Plant / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012996:Solutions; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D020011:Protective Agents; D026361:Reactive Nitrogen Species; D012116:Resins, Plant; D012996:Solutions