title: The expression of adiponectin receptor is altered in mild and moderate/severe persistent allergic nasal mucosa.
abstract: BACKGROUND
  Adiponectin, one of the adipokines, has been implicated in the inflammatory process in patients with allergic rhinitis. The level of adiponectin is affected by immunotherapy. Considering the fact that adiponectin receptors (AdipoRs) mediate intracellular signaling events in response to the binding of adiponectin, the role of AdipoRs in healthy and allergic nasal mucosa should be determined. This study investigates the level of expression and distribution pattern of AdipoR1 and AdipoR2 in healthy, mild, and moderate/severe persistent allergic nasal mucosa to understand the role of adiponectin in allergic rhinitis.
  
  
  METHODS
  The level of expression and distribution pattern of AdipoR1 and AdipoR2 were evaluated in healthy, mild, and moderate/severe persistent allergic nasal mucosa, using semiquantitative reverse-transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot analysis.
  
  
  RESULTS
  AdipoR1 was expressed in healthy, mild, and moderate/severe persistent allergic nasal mucosa where it was commonly localized to the vascular endothelium. However, AdipoR2 was not expressed in any samples of nasal mucosa tested in the present study. Semiquantitative RT-PCR and Western blot analysis showed that the level of expression of AdipoR1 mRNA and protein was decreased in mild and moderate/severe persistent allergic nasal mucosa in comparison with healthy nasal mucosa, but not significantly different between mild and moderate/severe persistent allergic nasal mucosa.
  
  
  CONCLUSIONS
  These results indicate that AdipoR1 may play an important role in the pathogenesis of allergic nasal mucosa, suggesting a role for AdipoR1 in vascular dysfunction in mild and moderate/severe persistent allergic nasal mucosa.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Ha Kyun
authors.initials: HK
authors.lastname: Kim
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Kim
authors.forename: Ki Hyoung
authors.initials: KH
authors.lastname: Kim
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Hwang
authors.forename: Sang Heon
authors.initials: SH
authors.lastname: Park
authors.forename: Gun Whee
authors.initials: GW
authors.lastname: Yum
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Woo Sung
authors.initials: WS
authors.lastname: Cho
authors.forename: Heung Man
authors.initials: HM
authors.lastname: Lee
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang Hag
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000503:physiopathology; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism*
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D015415:Biomarkers; D054419:Receptors, Adiponectin; D007073:Immunoglobulin E