title: Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli.
abstract: Establishment of a simple and rapid multiplex PCR system for identification of the main diarrheagenic E. coli categories, including enteroaggregative E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, and enterohemorrhagic E. coli, is described. This two-step multiplex PCR system allows the identification by targeting CVD432, LT, STh, STp, Eae, Bfp, Stx1, and Stx2. By applying the developed multiplex PCR system, categorization of E. coli isolates isolated from stool samples of infants with diarrhea into the main diarrheagenic E. coli categories is also shown.
authors.affiliation: Department of Microbiology and Immunology, University of Gothenburg, P.O. Box 435, S-40530 Gothenburg, Sweden. Joshua.Tobias@microbio.gu.se
authors.forename: Joshua
authors.initials: J
authors.lastname: Tobias
authors.forename: Sreekanth-Reddy
authors.initials: SR
authors.lastname: Vutukuru
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins