title: Proliferative enteropathy (PE)-induced changes in the calbindin-immunoreactive (CB-IR) neurons of inferior mesenteric ganglion supplying the descending colon in the pig.
abstract: A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the chemical coding patterns of these neurons during porcine proliferative enteropathy (PE). To answer this question, juvenile Large White Polish pigs with clinically diagnosed Lawsonia intracellularis infection (PE; n = 3) and a group of uninfected controls (C; n = 3) were compared. The retrograde tracer fast blue (FB) was injected into the descending colons of all animals and then tissue comprising IMGs from both groups was processed for double-labeling immunofluorescence with calbindin-D28k (CB) in combination with either tyrosine hydroxylase (TH), neuropeptide Y (NPY), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), nitric oxide synthase, Leu-enkephalin, substance P, vesicular acetylcholine transporter, galanin, or pituitary adenylate cyclase-activating polypeptide. Immunohistochemistry revealed changes in the chemical coding pattern of calbindin-immunoreactive neurons in the inferior mesenteric ganglia of the pig. In control animals, FB/CB-positive neurons were immunoreactive to TH, NPY, SOM, and VIP. In the experimental group, TH-expressing neurons were unaffected, NPY-expressing neurons were increased, whereas the number of neurons immunoreactive to SOM or VIP was reduced. Changes in chemical coding of CB neurons during PE may play an important role in adaptation of these IMG cells under pathological conditions.
authors.affiliation: Department of Neurology and Neurosurgery, Division of Neurosurgery, University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082 Olsztyn, Poland. Joanna.Wojtkiewicz@uwm.edu.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Wojtkiewicz
authors.forename: Maciej
authors.initials: M
authors.lastname: Równiak
authors.forename: Sławomir
authors.initials: S
authors.lastname: Gonkowski
authors.forename: Robert
authors.initials: R
authors.lastname: Crayton
authors.forename: Mariusz
authors.initials: M
authors.lastname: Majewski
authors.forename: Anna
authors.initials: A
authors.lastname: Robak
authors.forename: Joanna
authors.initials: J
authors.lastname: Białkowska
authors.forename: Monika
authors.initials: M
authors.lastname: Barczewska
mesh_terms: D000818:Animals; D064026:Calbindins; D002452:Cell Count; D044683:Colon, Descending / Q000737:chemistry / Q000294:innervation* / Q000473:pathology; D045824:Desulfovibrionaceae Infections / Q000473:pathology / Q000662:veterinary*; D017615:Enteric Nervous System / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000473:pathology*; D020639:Lawsonia Bacteria; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000737:chemistry / Q000145:classification / Q000473:pathology*; D009479:Neuropeptides / Q000032:analysis; D018377:Neurotransmitter Agents / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D017981:Receptors, Neurotransmitter / Q000032:analysis; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000473:pathology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D064026:Calbindins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D017981:Receptors, Neurotransmitter; D064030:S100 Calcium Binding Protein G; D019001:Nitric Oxide Synthase; D014446:Tyrosine 3-Monooxygenase