title: Proteomic identification of plasma biomarkers in uterine leiomyoma.
abstract: Recent progresses in quantitative proteomics have offered opportunities to discover plasma proteins as biomarkers for tracking the progression and for understanding the molecular mechanisms of uterine leiomyomas. In the present study, plasma samples were analyzed by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE) and differentially expressed proteins were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In total, 20 proteins have been firmly identified representing 13 unique gene products. These proteins mainly functioned in transportation (such as apolipoprotein A-I) and coagulation (such as fibrinogen gamma chain). Additionally, our quantitative proteomic approach has identified numerous previous reported plasma markers of uterine leiomyomas such as alpha-1-antitrypsin. On the contrary, we have presented several putative uterine leiomyomas biomarkers including afamin, apolipoprotein A-I, carbonic anhydrase 1, fibrinogen beta chain, fibrinogen gamma chain, gelsolin, hemopexin, leucine-rich alpha-2-glycoprotein, serotransferrin and vitamin D-binding protein which have not been reported and may be associated with the progression and development of the disease. In summary, we report a comprehensive patient-based proteomic approach for the identification of potential plasma biomarkers for uterine leiomyomas. The potential of utilizing these markers for screening and treating uterine leiomyomas warrants further investigations.
authors.affiliation: Department of Obstetrics and Gynecology, Chiayi Christian Hospital, Chiayi, Taiwan.
authors.forename: Chao-Po
authors.initials: CP
authors.lastname: Lin
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Liu
authors.forename: Hsiu-Chuan
authors.initials: HC
authors.lastname: Chou
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Chang
authors.forename: Szu-Ting
authors.initials: ST
authors.lastname: Lin
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Shiou-Fen
authors.initials: SF
authors.lastname: Jian
authors.forename: Ying-Ray
authors.initials: YR
authors.lastname: Lee
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Chan
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D002256:Carbonic Anhydrases / Q000097:blood; D005260:Female; D018260:Gelsolin / Q000097:blood; D006466:Hemopexin / Q000032:analysis; D006801:Humans; D007889:Leiomyoma / Q000097:blood / Q000175:diagnosis* / Q000503:physiopathology; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014168:Transferrin / Q000032:analysis; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods; D014809:Vitamin D-Binding Protein / Q000097:blood
chemical_list: D016632:Apolipoprotein A-I; D015415:Biomarkers; D001798:Blood Proteins; D018260:Gelsolin; D020543:Proteome; D014168:Transferrin; D014809:Vitamin D-Binding Protein; D006466:Hemopexin; D002256:Carbonic Anhydrases