title: Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter.
abstract: The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins.
authors.affiliation: Laboratory of Glyco-organic Chemistry, The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Tojino
authors.forename: Masako
authors.initials: M
authors.lastname: Mori
authors.forename: Maria Carmelita Z
authors.initials: MC
authors.lastname: Kasuya
authors.forename: Kenichi
authors.initials: K
authors.lastname: Hatanaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawaguchi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
authors.forename: Takashi
authors.initials: T
authors.lastname: Shirai
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D009980:Influenza A virus / Q000737:chemistry*; D008567:Membranes, Artificial; D009844:Oligosaccharides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D009844:Oligosaccharides; D011092:Polyethylene Glycols; D011138:Polytetrafluoroethylene