title: Studies on resources of genetic diversity in conservative flocks of geese using microsatellite DNA polymorphic markers.
abstract: The studies conducted aimed at evaluating the genetic diversity within and between varieties of conservative flocks of geese, using the polymorphism of 14 microsatellite sequences. The experimental material included conservative flocks of geese the following indigenous breeds and varieties kept in Poland: Kielecka (Ki), Kartuska (Ka), Lubelska (Lu), Suvalska (Su), Rypinska (Ry), Sub-Carpathian (SC), Hunched Beak (HB) and Pomeranian (Po). Among the 14 microsatellite sequences a total of 97 microsatellite alleles were identified. The number of alleles at one locus ranged from 3 to 19. In the overall pool of 97 alleles, 26 (26.8%) were specific for individual breeds and varieties of geese. The values of the expected heterozygosity (He) for individual geese ranged from 0.38 (Sub-Carpathian) to 0.51 (HB). Similarly, the mean values for the observed heterozygosity (Ho) ranged from 0.45 (Po) to 0.55 (Ki and Su). The polymorphic information content reached the highest value of 0.80 at loci CKW21 (Ki) and TTUCG5 (Po and Su). The greatest genetic distance was observed between the HB and Ry (0.44) and between the HB and Po (0.39) varieties, while the smallest-between the Lu and Po as well as Lu and Ki (0.028) varieties. The phylogenetic tree, elaborated on the basis of the genetic distances, clearly confirms the specificity of the HB goose as compared to the remaining breeds and varieties.
authors.affiliation: Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzebiec, 05-552, Wólka Kosowska, Poland. R.Parada@ighz.pl
authors.forename: Rafał
authors.initials: R
authors.lastname: Parada
authors.forename: Juliusz
authors.initials: J
authors.lastname: Książkiewicz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kawka
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Jaszczak
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D005777:Geese / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D007178:Inbreeding; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers