title: The link between antioxidant enzymes catalase and glutathione S-transferase and physiological condition of a control population of terrestrial isopod (Porcellio scaber).
abstract: The aim of this work was to investigate if the activities of catalase and glutathione S-transferase in a control population of terrestrial isopods (Porcellio scaber) are correlated with the physiological condition of the isopods. For this purpose, the activities of these enzymes were analysed in isopods from a stock population and in parallel, the physiological condition of the same specimens was assessed using a histological approach based on epithelial thickness and lipid droplets. We found a correlation between antioxidant enzymes and the physiological condition of the isopods. This implies that these enzymes could be used as predictive indicators of the physiological condition in a stock population before comprehensive toxicological studies are conducted and also in control group after the experiment. When a control group is found to be very heterogeneous in terms of physiological condition, the experiment should be repeated with a larger number of experimental animals. The findings of this study will contribute to more accurate experimental design of toxicity tests when using biomarkers. This should encourage other researchers to increase their effort to know the physiological state of their test organisms.
authors.affiliation: National Institute of Chemistry, Laboratory for Environmental Sciences and Engineering, Hajdrihova 19, SI-1000 Ljubljana, Slovenia. Electronic address: Anita.jemec@ki.si.
authors.forename: Anita
authors.initials: A
authors.lastname: Jemec
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, SI-1000 Ljubljana, Slovenia.
authors.forename: Vladka
authors.initials: V
authors.lastname: Lešer
authors.affiliation: University of Ljubljana, Biotechnical Faculty, Department of Biology, Večna pot 111, SI-1000 Ljubljana, Slovenia.
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D033321:Isopoda / Q000201:enzymology* / Q000502:physiology; D008297:Male; D012989:Soil Pollutants / Q000633:toxicity; D013312:Stress, Physiological; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D015415:Biomarkers; D012989:Soil Pollutants; D002374:Catalase; D005982:Glutathione Transferase