title: Application of IGF2-specific identifier probe for cytogenetic study of somatic and sperm cells in horses.
abstract: The development of molecular techniques with fluorochromes has had an invaluable impact on discovering the nature of chromatin structure. Here, we show the application of a locus specific identifier probe (LSI) for precise and selective visualization of the horse IGF2 gene in the metaphase, interphase nuclei and sperm cells. Our study may be helpful for interpretation of results of interphase fluorescence in situ hybridization (I-FISH). We analyze and discuss the variation in the number and localization of FISH signals in somatic and sperm cells of horse.
authors.affiliation: Department of Microbiology and Genetics of Microorganisms, University of Rzeszow, Rejtana 16C, 35-959 Rzeszów, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Potocki
authors.forename: Monika
authors.initials: M
authors.lastname: Bugno-Poniewierska
authors.forename: Marek
authors.initials: M
authors.lastname: Tischner
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D000818:Animals; D006736:Horses / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
chemical_list: D007335:Insulin-Like Growth Factor II