title: Multivitamin and mineral supplementation in 1,2-dimethylhydrazine induced experimental colon carcinogenesis and evaluation of free radical status, antioxidant potential, and incidence of ACF.
abstract: This study was performed to determine the chemopreventive and antioxidant status of multivitamin and mineral (0.01% in drinking water, ad libitum) supplements in 1,2-dimethylhydrazine (DMH)-induced experimental colon carcinogenesis. Experimental colon carcinogenesis was induced in male albino Wistar rats by injecting DMH (20 mg·(kg body mass)(-1)) once weekly for 15 consecutive weeks, and administering a multivitamin supplement in 3 regimes (initiation, post-initiation, and entire experimental period) for 32 weeks. We studied lipid peroxidation products (thiobarbituric acid reactive substances, lipid hydroperoxides, conjugated dienes) in the circulation and in the tissues, antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and non-enzymatic antioxidant-reduced glutathione) of the tissues, aberrant crypt foci (ACF), and histopathological alterations. DMH-induced rats had an increase in lipid peroxidation products and a lower antioxidant status compared with control animals. Multivitamin and mineral supplementation during the initiation, post-initiation, and the entire study period significantly decreased the levels of lipid peroxidation products in circulation and colonic tissues, significantly elevated the activities of the antioxidant enzymes and reduced glutathione to near normalcy in DMH-induced rats. The incidence of ACF was reduced by [corrected] 84.1% in rats supplemented with multivitamin and minerals for the entire study and prevented the colonic tissue from histopathological alterations induced by DMH.
authors.affiliation: Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
authors.forename: Albert Baskar
authors.initials: AB
authors.lastname: Arul
authors.forename: Ignacimuthu
authors.initials: I
authors.lastname: Savarimuthu
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Alsaif
authors.forename: Khalid S
authors.initials: KS
authors.lastname: Al Numair
mesh_terms: D019813:1,2-Dimethylhydrazine; D058739:Aberrant Crypt Foci / Q000178:diet therapy*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D004334:Drug Administration Schedule; D004338:Drug Combinations; D004354:Drug Screening Assays, Antitumor / Q000379:methods / Q000706:statistics & numerical data; D016166:Free Radical Scavengers / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009930:Organic Chemicals / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016588:Anticarcinogenic Agents; D000975:Antioxidants; D004338:Drug Combinations; D016166:Free Radical Scavengers; D009930:Organic Chemicals; C086404:Supradyn; D019813:1,2-Dimethylhydrazine