title: Biomedical applications of the ESRF synchrotron-based microspectroscopy platform.
abstract: Very little is known about the sub-cellular distribution of metal ions in cells. Some metals such as zinc, copper and iron are essential and play an important role in the cell metabolism. Dysfunctions in this delicate housekeeping may be at the origin of major diseases. There is also a prevalent use of metals in a wide range of diagnostic agents and drugs for the diagnosis or treatment of a variety of disorders. This is becoming more and more of a concern in the field of nanomedicine with the increasing development and use of nanoparticles, which are suspected of causing adverse effects on cells and organ tissues. Synchrotron-based X-ray and Fourier-transformed infrared microspectroscopies are developing into well-suited sub-micrometer analytical tools for addressing new problems when studying the role of metals in biology. As a complementary tool to optical and electron microscopes, developments and studies have demonstrated the unique capabilities of multi-keV microscopy: namely, an ultra-low detection limit, large penetration depth, chemical sensitivity and three-dimensional imaging capabilities. More recently, the capabilities have been extended towards sub-100nm lateral resolutions, thus enabling sub-cellular chemical imaging. Possibilities offered by these techniques in the biomedical field are described through examples of applications performed at the ESRF synchrotron-based microspectroscopy platform (ID21 and ID22 beamlines).
authors.affiliation: Inserm U-836, Team 6, Rayonnement Synchrotron et Recherche Médicales, Grenoble Institut des Neurosciences, Grenoble 38042, France. bohic@esrf.fr
authors.forename: S
authors.initials: S
authors.lastname: Bohic
authors.forename: M
authors.initials: M
authors.lastname: Cotte
authors.forename: M
authors.initials: M
authors.lastname: Salomé
authors.forename: B
authors.initials: B
authors.lastname: Fayard
authors.forename: M
authors.initials: M
authors.lastname: Kuehbacher
authors.forename: P
authors.initials: P
authors.lastname: Cloetens
authors.forename: G
authors.initials: G
authors.lastname: Martinez-Criado
authors.forename: R
authors.initials: R
authors.lastname: Tucoulou
authors.forename: J
authors.initials: J
authors.lastname: Susini
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D020811:Biomedical Technology; D059290:Dopaminergic Neurons / Q000378:metabolism; D005602:France; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008297:Male; D008345:Manganese / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D051379:Mice; D015591:Microspectrophotometry / Q000379:methods; D016716:PC12 Cells; D010758:Phosphorus / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013094:Spermatozoa / Q000378:metabolism; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D008543:Melanins; D008670:Metals; C014121:neuromelanin; D010758:Phosphorus; D008345:Manganese; D011188:Potassium