title: Influence of dental exposure to oral environment on smear layer removal and collagen exhibition after using different conditioning agents.
abstract: Although in vitro studies have shown encouraging results for root surface conditioning with demineralizing agents, in vivo studies have failed to show its benefits in periodontal healing. This can be attributed to several factors, among which, the hypermineralization of dental surface. Therefore, this in vitro study compared, using scanning electron microscopy (SEM), the effect of root surface conditioning with different conditioners (1% and 25% citric acid, 24% EDTA and 50 mg/mL tetracycline hydrochloride) in impacted teeth and in teeth that had their roots exposed to the oral environment. One trained examiner assessed the SEM micrographs using a root surface modification index. There was a tendency of more root surface modification in the group of impacted teeth, suggesting that the degree of root mineralization influences its chemical demineralization.
authors.affiliation: Department of Oral Diagnosis and Surgery, Araraquara Dental School, Univ Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Lucas Amaral
authors.initials: LA
authors.lastname: Fontanari
authors.forename: Shelon Cristina Souza
authors.initials: SC
authors.lastname: Pinto
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cavassim
authors.forename: Rubens
authors.initials: R
authors.lastname: Spin-Neto
authors.forename: Eduardo de Paula
authors.initials: Ede P
authors.lastname: Ishi
authors.forename: José Eduardo Cezar
authors.initials: JE
authors.lastname: Sampaio
mesh_terms: D000328:Adult; D002614:Chelating Agents / Q000627:therapeutic use; D019343:Citric Acid / Q000008:administration & dosage / Q000627:therapeutic use; D003094:Collagen / Q000187:drug effects / Q000648:ultrastructure*; D003728:Dental Calculus / Q000473:pathology; D003739:Dental Cementum / Q000187:drug effects / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure*; D004492:Edetic Acid / Q000627:therapeutic use; D005884:Gingival Hemorrhage / Q000473:pathology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010514:Periodontal Pocket / Q000473:pathology; D016745:Root Planing / Q000379:methods*; D016369:Smear Layer; D013752:Tetracycline / Q000627:therapeutic use; D014092:Tooth Root / Q000187:drug effects / Q000648:ultrastructure*; D014095:Tooth, Impacted / Q000473:pathology
chemical_list: D002614:Chelating Agents; D019343:Citric Acid; D003094:Collagen; D004492:Edetic Acid; D013752:Tetracycline