title: Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker.
abstract: The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia no cleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loaches.
authors.affiliation: Department of Zoology, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland. leo@uwm.edu.pl
authors.forename: Lech
authors.initials: L
authors.lastname: Kirtiklis
authors.forename: Alicja
authors.initials: A
authors.lastname: Boroń
authors.forename: Piotr
authors.initials: P
authors.lastname: Ptasznik
authors.forename: Vera
authors.initials: V
authors.lastname: Lusková
authors.forename: Stanislav
authors.initials: S
authors.lastname: Lusk
mesh_terms: D000818:Animals; D001483:Base Sequence; D003531:Cypriniformes / Q000145:classification / Q000235:genetics*; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D005819:Genetic Markers