title: Liquid crystal-based immunoassays for detecting hepatitis B antibody.
abstract: Conventional immunoassays are often fluorescence- or enzyme-based and require additional readout systems. Here, we report a liquid crystal (LC)-based immunoassay that features a new signal transduction mechanism, whereby the test results appear as colorful spots and can be viewed with the naked eye directly. By using the different colors of LC spots, we can estimate the range of anti-hepatitis B surface antigen concentrations between 300 and 15 nM. Because additional labeling process and readout systems are not required in this immunoassay, it is more suitable for point-of-care diagnosis of hepatitis B in resource-limited regions.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore.
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Chen
authors.forename: Kun-Lin
authors.initials: KL
authors.lastname: Yang
mesh_terms: D000818:Animals; D006509:Hepatitis B / Q000175:diagnosis / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D050866:Liquid Crystals; D051379:Mice; D055620:Optical Phenomena; D019095:Point-of-Care Systems
chemical_list: D006510:Hepatitis B Antibodies