title: Accurate proteome-wide protein quantification from high-resolution 15N mass spectra.
abstract: In quantitative mass spectrometry-based proteomics, the metabolic incorporation of a single source of 15N-labeled nitrogen has many advantages over using stable isotope-labeled amino acids. However, the lack of a robust computational framework for analyzing the resulting spectra has impeded wide use of this approach. We have addressed this challenge by introducing a new computational methodology for analyzing 15N spectra in which quantification is integrated with identification. Application of this method to an Escherichia coli growth transition reveals significant improvement in quantification accuracy over previous methods.
authors.affiliation: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Washington Rd., Princeton, NJ 08544, USA.
authors.forename: Zia
authors.initials: Z
authors.lastname: Khan
authors.forename: Sasan
authors.initials: S
authors.lastname: Amini
authors.forename: Joshua S
authors.initials: JS
authors.lastname: Bloom
authors.forename: Cristian
authors.initials: C
authors.lastname: Ruse
authors.forename: Amy A
authors.initials: AA
authors.lastname: Caudy
authors.forename: Leonid
authors.initials: L
authors.lastname: Kruglyak
authors.forename: Mona
authors.initials: M
authors.lastname: Singh
authors.forename: David H
authors.initials: DH
authors.lastname: Perlman
authors.forename: Saeed
authors.initials: S
authors.lastname: Tavazoie
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis*; D004926:Escherichia coli; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D001426:Bacterial Proteins; D009587:Nitrogen Isotopes; D020543:Proteome