title: Structure of an antibody in complex with its mucin domain linear epitope that is protective against Ebola virus.
abstract: Antibody 14G7 is protective against lethal Ebola virus challenge and recognizes a distinct linear epitope in the prominent mucin-like domain of the Ebola virus glycoprotein GP. The structure of 14G7 in complex with its linear peptide epitope has now been determined to 2.8 Å. The structure shows that this GP sequence forms a tandem β-hairpin structure that binds deeply into a cleft in the antibody-combining site. A key threonine at the apex of one turn is critical for antibody interaction and is conserved among all Ebola viruses. This work provides further insight into the mechanism of protection by antibodies that target the protruding, highly accessible mucin-like domain of Ebola virus and the structural framework for understanding and characterizing candidate immunotherapeutics.
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Olal
authors.forename: Ana I
authors.initials: AI
authors.lastname: Kuehne
authors.forename: Shridhar
authors.initials: S
authors.lastname: Bale
authors.forename: Peter
authors.initials: P
authors.lastname: Halfmann
authors.forename: Takao
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Marnie L
authors.initials: ML
authors.lastname: Fusco
authors.forename: Jeffrey E
authors.initials: JE
authors.lastname: Lee
authors.forename: Liam B
authors.initials: LB
authors.lastname: King
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: John M
authors.initials: JM
authors.lastname: Dye
authors.forename: Erica Ollmann
authors.initials: EO
authors.lastname: Saphire
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry* / Q000276:immunology*; D001666:Binding Sites, Antibody; D029043:Ebolavirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006801:Humans; D055029:Inverted Repeat Sequences; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus