title: Chemical contrast in soft x-ray ptychography.
abstract: The unique strengths of x-ray microscopy are high penetration depth and near-edge resonances that provide chemical information. We use ptychography, a coherent diffractive imaging technique that disposes of the requirement for isolated specimens, and demonstrate resonant imaging by exploiting resonances near the oxygen K edge to differentiate between two oxygen-containing materials. To highlight a biological system where resonant ptychography might be used for chemical mapping of unsliced cells, reconstructions of freeze-dried Deinococcus radiodurans cells at an energy of 517 eV are shown.
authors.affiliation: Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany. beckers@uni-heidelberg.de
authors.forename: Mike
authors.initials: M
authors.lastname: Beckers
authors.forename: Tobias
authors.initials: T
authors.lastname: Senkbeil
authors.forename: Thomas
authors.initials: T
authors.lastname: Gorniak
authors.forename: Michael
authors.initials: M
authors.lastname: Reese
authors.forename: Klaus
authors.initials: K
authors.lastname: Giewekemeyer
authors.forename: Sophie-Charlotte
authors.initials: SC
authors.lastname: Gleber
authors.forename: Tim
authors.initials: T
authors.lastname: Salditt
authors.forename: Axel
authors.initials: A
authors.lastname: Rosenhahn
mesh_terms: D034301:Deinococcus / Q000166:cytology; D010100:Oxygen / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D012822:Silicon Dioxide; D019904:Polymethyl Methacrylate; D010100:Oxygen