title: Might cardiac catheterization influence diurnal rhythm of the steroid stress hormones secretion?
abstract: Glucocorticoids (GCs) are steroid hormones produced by the adrenal cortex in reaction to stress stimuli. GCs production is not stable over a 24-hour period; the plasma concentration peaks in the morning (approximately upon awakening) and then the plasma levels decrease, reaching the nadir in the evening. In our experiments, the levels of cortisol, cortisone, DHEA and DHEAS were tested in young female pigs (n=23) during heart catheterization at two different day times (in the morning and in the afternoon). The non-parametric Mann-Whitney test for statistical analysis was used. We found only minimal statistical differences in studied markers between the morning and afternoon group (p>0.05). The absence of circadian variation in GCs levels could originate either at an early age of our experimental pigs, or in stressful conditions on the experiment day, or most likely the day before (e.g. social isolation, fasting, transport, and catheterization), respectively. We can conclude there is no difference in the stress load between morning and afternoon experiments, and therefore we can assume the stress load is not a limiting factor for the timing when catheterization should be preferably performed.
authors.affiliation: Institute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Skarlandtová
authors.forename: M
authors.initials: M
authors.lastname: Bičíková
authors.forename: P
authors.initials: P
authors.lastname: Neužil
authors.forename: M
authors.initials: M
authors.lastname: Mlček
authors.forename: V
authors.initials: V
authors.lastname: Hrachovina
authors.forename: T
authors.initials: T
authors.lastname: Svoboda
authors.forename: E
authors.initials: E
authors.lastname: Medová
authors.forename: J
authors.initials: J
authors.lastname: Kudlička
authors.forename: A
authors.initials: A
authors.lastname: Dohnalová
authors.forename: S
authors.initials: S
authors.lastname: Havránek
authors.forename: H
authors.initials: H
authors.lastname: Kazihnítková
authors.forename: L
authors.initials: L
authors.lastname: Máčová
authors.forename: E
authors.initials: E
authors.lastname: Vařejková
authors.forename: O
authors.initials: O
authors.lastname: Kittnar
mesh_terms: D000305:Adrenal Cortex Hormones / Q000097:blood*; D000818:Animals; D006328:Cardiac Catheterization / Q000523:psychology*; D002940:Circadian Rhythm / Q000502:physiology*; D003348:Cortisone / Q000097:blood; D003687:Dehydroepiandrosterone / Q000097:blood; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D013256:Steroids / Q000097:blood*; D013315:Stress, Psychological / Q000097:blood*; D013552:Swine
chemical_list: D000305:Adrenal Cortex Hormones; D013256:Steroids; D003687:Dehydroepiandrosterone; D003348:Cortisone; D006854:Hydrocortisone