title: Global and local hard X-ray tomography of a centimeter-size tumor vessel tree.
abstract: The visualization of the vascular network in tumors down to the smallest vessels requires high spatial resolution and reasonable contrast. Stained corrosion casts of the microvasculature network guarantee superior X-ray absorption contrast and highest reproduction fidelity. Tomography of a centimeter-size tumor, however, is unfeasible at the spatial resolution needed to reveal the smallest vessels. Therefore, local tomography has been performed to visualize the smallest capillaries within the region of interest. These three-dimensional data show the detailed morphology, but the reconstructed absorption coefficients obtained in local tomography differ substantially from the absorption coefficients retrieved from the less detailed global tomography data. This paper deals with the adaptation of local tomography data using the global data and considers two-parameter histogram matching of the radiographs, sinogram extension, and multi-parameter cupping correction. It is demonstrated that two-parameter histogram matching of the radiographs already provides reasonable agreement. The change of the lens in front of the detector's camera, however, significantly affects the obtained local X-ray absorption coefficients in the tomograms predominantly owing to the dissimilar point-spread functions of the two configurations used, and much less to the fact that one of the data sets was acquired in a local geometry.
authors.affiliation: Biomaterials Science Center, University of Basel, Basel, Switzerland.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Lang
authors.forename: Marco
authors.initials: M
authors.lastname: Dominietto
authors.forename: Philippe
authors.initials: P
authors.lastname: Cattin
authors.forename: Alexandra
authors.initials: A
authors.lastname: Ulmann-Schuler
authors.forename: Timm
authors.initials: T
authors.lastname: Weitkamp
authors.forename: Bert
authors.initials: B
authors.lastname: Müller
mesh_terms: D000818:Animals; D002196:Capillaries / Q000033:anatomy & histology; D003110:Colonic Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D017356:Synchrotrons; D014057:Tomography, X-Ray Computed / Q000379:methods; D055114:X-Ray Microtomography / Q000379:methods