title: Discrete foci containing RNase A are found in nucleoli of HeLa cells after aging in culture.
abstract: We have studied by means of ultrastructural immunocytochemistry the localization of RNase A in nuclei of HeLa cells in control conditions and following cell ageing in culture. We have found that roundish, electron dense foci, which contain a significant amount of RNase A, can be detected within nucleoli of aged cells. These bodies also contain RNA and lack ribosomal S3 proteins, and may represent either simple storage sites or areas where RNA degradation takes place.
authors.affiliation: Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Università di Pavia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Costanzo
authors.forename: B
authors.initials: B
authors.lastname: Cisterna
authors.forename: O O
authors.initials: OO
authors.lastname: Zharskaya
authors.forename: O V
authors.initials: OV
authors.lastname: Zatsepina
authors.forename: M
authors.initials: M
authors.lastname: Biggiogera
mesh_terms: D002466:Cell Nucleolus / Q000201:enzymology*; D016922:Cellular Senescence / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D012313:RNA / Q000378:metabolism; D020871:RNA Stability / Q000502:physiology; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*
chemical_list: D012313:RNA; D012259:Ribonuclease, Pancreatic