title: 17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules.
abstract: Spermatogenesis, which is the fundamental mechanism allowing male gamete production, is controlled by several factors, and among them, estrogens are likely concerned. In order to enlighten the potential role of estrogen in rat spermatogenesis, seminiferous tubules (ST) from two groups of seminiferous epithelium stages (II-VIII and IX-I) were treated with either 17β-estradiol (E(2)) agonists or antagonists for estrogen receptors (ESRs). In this study, we show that cyclin A1 and cyclin B1 gene expression is controlled by E(2) at a concentration of 10(-9) M only in stages IX-I. This effect is mimicked by a treatment with the G-protein coupled estrogen receptor (GPER) agonist G1 and is abolished by treatment with the ESR antagonist ICI 182 780. Moreover, using letrozole, a drug that blocks estrogen synthesis, we demonstrate that these genes are under the control of E(2) within rat ST. Thus, germ cell differentiation may be regulated by E(2) which acts through ESRs and GPER, expressed in adult rat ST.
authors.affiliation: Université de Caen Basse Normandie, EA 2608, Laboratoire Estrogènes et Reproduction, Esplanade de la Paix, F-14032 Caen Cedex, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Bois
authors.forename: Christelle
authors.initials: C
authors.lastname: Delalande
authors.forename: Hélène
authors.initials: H
authors.lastname: Bouraïma-Lelong
authors.forename: Philippe
authors.initials: P
authors.lastname: Durand
authors.forename: Serge
authors.initials: S
authors.lastname: Carreau
mesh_terms: D000818:Animals; D047072:Aromatase Inhibitors / Q000494:pharmacology; D056750:Cyclin A1 / Q000235:genetics / Q000378:metabolism; D056744:Cyclin B1 / Q000235:genetics / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D000077267:Fulvestrant; D015870:Gene Expression / Q000187:drug effects*; D000077289:Letrozole; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013091:Spermatogenesis / Q000187:drug effects* / Q000502:physiology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D047072:Aromatase Inhibitors; D056750:Cyclin A1; D056744:Cyclin B1; D004965:Estrogen Antagonists; D009570:Nitriles; D011960:Receptors, Estrogen; D014230:Triazoles; D000077267:Fulvestrant; D004958:Estradiol; D000077289:Letrozole