title: Arsenate V induced glutathione efflux from human erythrocytes.
abstract: OBJECTIVE
  The objective of the present study was to investigate if arsenate V exposure results in glutathione efflux from human erythrocytes.
  
  
  METHODS
  The changes in intracellular and extracellular nonprotein sulfhydryl and glutathione levels were determined in arsenate (V) exposed erythrocytes. Presence of any cellular membrane damage was assessed by lactate dehydrogenase activity measurement in the supernatant.
  
  
  RESULTS
  When erythrocytes were exposed to 10 mM of arsenate (V) for 4 h, the intracellular NPSH level decreased to 0.28±0025 μmol/ml erythrocyte. In contrast, extracellular nonprotein thiol level was increased to 0.180±0.010 μmol/ml erythrocyte in 4 h. Extracellular glutathione levels reached to 0.028±0.001, 0.052±0.002, and 0.054±0.004 μmol/ml erythrocyte with 1, 5, and 10 mM of arsenate (V), respectively. Utilization of MK571 a multi drug resistance-associated protein 1 inhibitor decreased the rate of glutathione efflux from erythrocytes suggesting a role for this membrane transporter in the process.
  
  
  CONCLUSIONS
  The results of the present study indicate that erythrocytes efflux glutathione when exposed to arsenate (V).
authors.affiliation: Mustafa Kemal University, Faculty of Arts and Science, Biology Department, 31000 Antakya, Turkey. dyildiz@mku.edu.tr
authors.forename: Deniz
authors.initials: D
authors.lastname: Yildiz
authors.forename: Yeliz
authors.initials: Y
authors.lastname: Cakir
mesh_terms: D001149:Arsenates / Q000633:toxicity*; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D001149:Arsenates; C009277:sodium arsenate; D006836:Hydro-Lyases; C045581:lactate dehydratase; D005978:Glutathione