title: The protective effect of EGB761 on vessels of denervated gastrocnemius in rats and its mechanism.
abstract: This study investigated the protective effect of EGB761 on blood vessels of denervated gastrocnemius of rat and its possible mechanism. Fifteen male adult SD rats were randomly divided into three groups: normal control group (n=3), control group (n=6) and EGB761-treated group (n=6). The rats in the control and EGB761-treated group underwent a neurotomy to bilateral sciatic nerves. Then, they were administered EGB761 [100 mg/(kg·d)] and isovolumic normal saline, respectively by gavage everyday. No treatment was given to the rats in the normal control group. Gastrocnemius was harvested at 1 and 3 week(s) postoperatively in each group. Immunohistochemical method was used to detect the ratio of capillary/fiber (CFR) of denervated gastrocnemius and the expression of VEGF, fetal liver kinase -1(Flk-1) receptor and HSP70 in the vascular wall. The results showed that in the normal control group, VEGF, Flk-1 and HSP70 were expressed in the vessel wall of gastrocnemius, with Flk-1 expressed only in the endothelial cell of vessels. CFR in the EGB761-treated group was significantly higher than that in the control group at 1 week and 3 week(s) after neurotomy. The expression of VEGF and Flk-1 in the vessel wall of both control and EGB761-treated group was much lower than that in the normal control group, and the expression of these proteins in the EGB761-treated group was decreased as compared with that in the control group. The expression of HSP70 in the vessel wall of both control and EGB761-treated groups was enhanced when compared with that in the normal control group, and it was substantially augmented in the EGB761-treated group in comparison to the control group. It was concluded that EGB761 has a protective effect on blood vessels of denervated gastrocnemius, which is related to the increased HSP70 expression but not the expression of VEGF and its receptor Flk-1.
authors.affiliation: Chinese PLA Postgraduate Medical School, Beijing, 100853, China.|Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou, 450000, China.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Cardiopulmonary Function, Henan Provincial People's Hospital, Zhengzhou, 450000, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. kanghao100@vipsina.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Guangxiang
authors.initials: G
authors.lastname: Hong
authors.affiliation: Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou, 450000, China.
authors.forename: Shensong
authors.initials: S
authors.lastname: Kang
authors.affiliation: Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou, 450000, China.
authors.forename: Zhengwen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002317:Cardiovascular Agents / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D009121:Muscle Denervation / Q000379:methods*; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000294:innervation*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000601:surgery; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D000096983:Ginkgo Extract
chemical_list: D002317:Cardiovascular Agents; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts; D020011:Protective Agents; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2; C467498:vascular endothelial growth factor A, rat; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract