title: Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus.
abstract: OBJECTIVE
  To test the feasibility of identifying Staphylococcus aureus with a fluorescence in situ hybridization (FISH) assay that uses a single hot-plate and urea-NaCl reagents.
  
  
  RESULTS
  Slides spotted with S. aureus and treated with methanol and lysozyme were incubated with urea-NaCl reagents on a hot-plate with a precise temperature control and identified with specific DNA probes.
  
  
  CONCLUSIONS
  Staphylococcus aureus was detected and differentiated from Staphylococcus epidermidis in 1 h with a novel FISH method that used a single hot-plate and in the absence of dimethyl formamide.
  
  
  CONCLUSIONS
  A rapid hot-plate FISH assay with urea-NaCl and without toxic dimethyl formamide might be useful if FISH is run infrequently or where resources are limited.
authors.affiliation: Faculty of Science, Macquarie University, NSW, Australia. tomxlawson@gmail.com
authors.forename: T S
authors.initials: TS
authors.lastname: Lawson
authors.forename: R E
authors.initials: RE
authors.lastname: Connally
authors.forename: S
authors.initials: S
authors.lastname: Vemulpad
authors.forename: J A
authors.initials: JA
authors.lastname: Piper
mesh_terms: D015342:DNA Probes; D004126:Dimethylformamide; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D000432:Methanol; D009113:Muramidase; D012965:Sodium Chloride / Q000737:chemistry*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D014508:Urea / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D012965:Sodium Chloride; D004126:Dimethylformamide; D014508:Urea; D009113:Muramidase; D000432:Methanol