title: Titration of feline immunodeficiency virus-based lentiviral vector preparations.
abstract: Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes methods for measuring and calculating vector titers in transducing units (TU)/mL. Alternate methods are provided for green fluorescent protein (GFP) vectors and for β-galactosidase vectors.
authors.forename: Dyana T
authors.initials: DT
authors.lastname: Saenz
authors.forename: Román
authors.initials: R
authors.lastname: Barraza
authors.forename: Nils
authors.initials: N
authors.lastname: Loewen
authors.forename: Wulin
authors.initials: W
authors.lastname: Teo
authors.forename: Eric M
authors.initials: EM
authors.lastname: Poeschla
mesh_terms: D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D019562:Viral Load / Q000379:methods*