title: Synthesis of Fe3O4@phenol formaldehyde resin core-shell nanospheres loaded with Au nanoparticles as magnetic FRET nanoprobes for detection of thiols in living cells.
abstract: A magnetic, sensitive, and selective fluorescence resonance energy transfer (FRET) probe for detection of thiols in living cells was designed and prepared. The FRET probe consists of an Fe(3)O(4) core, a green-luminescent phenol formaldehyde resin (PFR) shell, and Au nanoparticles (NPs) as FRET quenching agent on the surface of the PFR shell. The Fe(3)O(4) NPs were used as the core and coated with green-luminescent PFR nanoshells by a simple hydrothermal approach. Au NPs were then loaded onto the surface of the PFR shell by electric charge absorption between Fe(3)O(4)@PFR and Au NPs after modifying the Fe(3)O(4)@PFR nanocomposites with polymers to alter the charge of the PFR shell. Thus, a FRET probe can be designed on the basis of the quenching effect of Au NPs on the fluorescence of Fe(3)O(4)@PFR nanocomposites. This magnetic and sensitive FRET probe was used to detect three kinds of primary biological thiols (glutathione, homocysteine, and cysteine) in cells. Such a multifunctional fluorescent probe shows advantages of strong magnetism for sample separation, sensitive response for sample detection, and low toxicity without injury to cellular components.
authors.affiliation: Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Xu
authors.forename: Sheng-Yu
authors.initials: SY
authors.lastname: Jin
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Yu
mesh_terms: D002477:Cells / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D005557:Formaldehyde / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D010636:Phenols; D011108:Polymers; D013438:Sulfhydryl Compounds; D005557:Formaldehyde; C000499:ferric oxide; D006046:Gold; C011529:phenol-formaldehyde resin