title: Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry.
abstract: In this study, a LC-based identification strategy was proposed and off-line two-dimensional liquid chromatography was developed for the separation and identification of flavonoids from complex samples. The highly orthogonal separation system was composed of a CD column, an OEG column and an XTerra C(18) column. The CD column was employed for the first dimensional separation and the OEG column and XTerra C(18) column were used for the second dimensional separation in parallel. A mixture of six traditional Chinese medicines was used as a complex sample in testing this method. Unknown peaks were identified by matching their retention times and accurate mass molecular weights with those of references. The identification efficacy of parallel unidimensional liquid chromatography and off-line two-dimensional liquid chromatography was compared, and the latter was demonstrated to be more efficient for the identification of target compounds-especially co-eluted compounds or minor compounds from complex samples.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Jiatao
authors.initials: J
authors.lastname: Feng
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zeng
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids