title: [Estimation of human and animal immunological memory by testing the trogocytosis of virus-specific T lymphocytes].
abstract: This study is the first attempt to evaluate the immunogenicity of Russian live attenuated influenza reassortant influenza vaccine (LAIV), by using a modified T-cell recognition of antigen presenting cells by protein capture (TRAP) method. Single vaccination of 18-20-year-old volunteers with LAIV causes an increase in the peripheral blood levels of virus-specific memory CD4+ T lymphocytes. Some (40-60%) LAIV-vaccination volunteers respond to immunization by showing a significant elevation in the peripheral blood level of memory CD4+ T cells without a systemic humoral immune response recorded in the passive hemagglutination test. Vaccination of mice with live attenuated A (H1N1) influenza reassortant virus stimulates the production of memory CD8+CD44hi T lymphocytes in the nasal-associated lymphoid tissue, the entry of infection, so does influenza infection. Vaccination with inactivated A (H1N1) influenza virus practically fails to induce these cells. A (H1N1) influenza virus-specific CD8+CD44hi T lymphocytes remain within at least 2 months (observation time). The authors' modified TRAP may be used to evaluate virus-specific immunological T-cell memory after vaccination.
authors.forename: A N
authors.initials: AN
authors.lastname: Naĭkhin
authors.forename: D A
authors.initials: DA
authors.lastname: Koren'kov
authors.forename: T V
authors.initials: TV
authors.lastname: Chirkova
authors.forename: G D
authors.initials: GD
authors.lastname: Petukhova
authors.forename: S A
authors.initials: SA
authors.lastname: Donina
authors.forename: L G
authors.initials: LG
authors.lastname: Rudenko
mesh_terms: D000293:Adolescent; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D023421:Models, Animal; D020545:Respiratory Mucosa / Q000276:immunology; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000276:immunology; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated