title: Carbohydrate intake modulates the effect of the ABCA1-R230C variant on HDL cholesterol concentrations in premenopausal women.
abstract: The R230C variant of the ATP-binding cassette transporter A1 (ABCA1) gene has been consistently associated with decreased HDL-cholesterol (HDL-C) concentrations in several studies in the Mexican mestizo population. However, information on how diet composition modifies the effect of the ABCA1-R230C variant on HDL-C concentrations is very scarce. The aim of the present study was to analyze whether the effect of ABCA1-R230C on HDL-C concentrations is modulated by dietary factors in a nationwide population sample of 3591 adults from the National Health and Nutrition Survey conducted by the State's Employees' Social Security and Social Services Institute. All participants answered a validated questionnaire to assess health status and weekly food consumption. Fasting blood samples were drawn for biochemical analysis and DNA extraction, and the ABCA1-R230C variant was genotyped using TaqMan assays. Statistical analyses consisted of simple linear and multiple regression modeling adjusting for age, BMI, smoking, and alcohol consumption. The overall C risk allele frequency was 9.3% and the variant was significantly associated with low HDL-C concentrations in both sexes. A significant negative correlation between carbohydrate consumption and HDL-C concentrations was observed in women bearing the R230C variant (P = 0.021) and a significant gene-diet interaction was found only in premenopausal women (P = 0.037). In conclusion, the effect of the ABCA1-R230C gene variant on HDL-C concentrations is modulated by carbohydrate intake in premenopausal women. This finding may help design optimized dietary interventions according to sex and ABCA1-R230C genotype.
authors.affiliation: Computational Genomics Department, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Hidalgo
authors.forename: Teresa
authors.initials: T
authors.lastname: Villarreal-Molina
authors.forename: Juan A
authors.initials: JA
authors.lastname: González-Barrios
authors.forename: Samuel
authors.initials: S
authors.lastname: Canizales-Quinteros
authors.forename: Martha E
authors.initials: ME
authors.lastname: Rodríguez-Arellano
authors.forename: Lucia B
authors.initials: LB
authors.lastname: Yañez-Velazco
authors.forename: Demetrio A
authors.initials: DA
authors.lastname: Bernal-Alcantara
authors.forename: Antonio R
authors.initials: AR
authors.lastname: Villa
authors.forename: Barbara
authors.initials: B
authors.lastname: Antuna-Puente
authors.forename: Víctor
authors.initials: V
authors.lastname: Acuña-Alonzo
authors.forename: José L
authors.initials: JL
authors.lastname: Merino-García
authors.forename: Hayde N
authors.initials: HN
authors.lastname: Moreno-Sandoval
authors.forename: Alessandra
authors.initials: A
authors.lastname: Carnevale
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D000328:Adult; D000483:Alleles; D008076:Cholesterol, HDL / Q000097:blood*; D003430:Cross-Sectional Studies; D004034:Diet Surveys; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D017697:Premenopause / Q000097:blood / Q000235:genetics; D012307:Risk Factors; D012727:Sex Characteristics; D011795:Surveys and Questionnaires
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; D004040:Dietary Carbohydrates