title: Isolation and identification of Concanavalin A binding glycoproteins from human seminal plasma: a step towards identification of male infertility marker proteins.
abstract: Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Tomar
authors.forename: Balwinder Singh
authors.initials: BS
authors.lastname: Sooch
authors.forename: Isha
authors.initials: I
authors.lastname: Raj
authors.forename: Sarman
authors.initials: S
authors.lastname: Singh
authors.forename: Tej P
authors.initials: TP
authors.lastname: Singh
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism*; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D056084:Genetic Fitness / Q000235:genetics; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011952:Receptors, Concanavalin A / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013081:Sperm Motility / Q000235:genetics
chemical_list: D015415:Biomarkers; D011952:Receptors, Concanavalin A; D029589:Seminal Plasma Proteins; C027774:concanavalin A-binding glycoproteins; D003208:Concanavalin A