title: A comparative study of the preventive effects of mitomycin C and chitosan on intraarticular adhesion after knee surgery in rabbits.
abstract: We sought to compare the preventive effects of mitomycin-C(MMC) and chitosan on intraarticular adhesion after knee surgery in rabbits. For this purpose, 48 New-Zealand rabbits were randomly and equally divided into MMC, chitosan, and control groups. Approximately 10 × 10 mm(2) of the cortical bone was removed from both sides of left femoral condyle and the cancellous bone underneath was exposed. The decorticated areas were topically treated with MMC and chitosan while control group was treated with physiological saline. The lower left limb was fixed in flexed position with Kirschner-wire for 4 weeks postoperatively. After 4 weeks, gross and histopathological examination, biochemical analysis, and fibroblast counts were performed on knee intraarticular adhesion in each group. The data show mild membrane-like fibrous intraarticular adhesion, presented in loose, in MMC group. There was moderate intraarticular adhesion in chitosan group while in controls; there was large-size compact fibrous tissue adhesion. Hydroxyproline contents and fibroblast quantity of MMC and chitosan groups were lower (P < 0.05) than that of control group. We, therefore, concluded that MMC and chitosan could prevent intraarticular adhesion of the knee in rabbits by inhibiting fibroblast proliferation and reducing collagenous fiber formation while MMC had a better preventive effect than that of chitosan.
authors.affiliation: Department of Cadiothoracic, The Second Xiangya Hospital Affiliated with Central-South University, No. 139A, Changsha, Hunan, China.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Wang
authors.forename: Lianqi
authors.initials: L
authors.lastname: Yan
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Daxin
authors.initials: D
authors.lastname: Wang
authors.forename: Shanhe
authors.initials: S
authors.lastname: Dai
authors.forename: Tangyun
authors.initials: T
authors.lastname: Yu
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Gu
authors.forename: Baichuan
authors.initials: B
authors.lastname: Jiang
authors.forename: Xinmin
authors.initials: X
authors.lastname: Feng
authors.forename: Hansheng
authors.initials: H
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Bangliang
authors.initials: B
authors.lastname: Yin
authors.forename: Guohua
authors.initials: G
authors.lastname: Lv
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D048271:Chitosan / Q000494:pharmacology*; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006909:Hydroxyproline / Q000378:metabolism; D007719:Knee Joint / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D011817:Rabbits; D000267:Tissue Adhesions / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000477:Alkylating Agents; D016685:Mitomycin; D048271:Chitosan; D006909:Hydroxyproline