title: Analysis of histones and histone variants in plants.
abstract: Histone proteins are the major protein components of chromatin - the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, UP, India.
authors.forename: Ila
authors.initials: I
authors.lastname: Trivedi
authors.forename: Krishan Mohan
authors.initials: KM
authors.lastname: Rai
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Verandra
authors.initials: V
authors.lastname: Kumar
authors.forename: Mala
authors.initials: M
authors.lastname: Singh
authors.forename: Amol
authors.initials: A
authors.lastname: Ranjan
authors.forename: Niraj
authors.initials: N
authors.lastname: Lodhi
authors.forename: Samir V
authors.initials: SV
authors.lastname: Sawant
mesh_terms: D000143:Acids; D015153:Blotting, Western; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D008967:Molecular Biology / Q000379:methods*; D008970:Molecular Weight; D050505:Mutant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D016622:Silver Staining
chemical_list: D000143:Acids; D006657:Histones; D050505:Mutant Proteins; D010940:Plant Proteins