title: EGF stimulates IClswell by a redistribution of proteins involved in cell volume regulation.
abstract: BACKGROUND
  ICln is a multifunctional protein involved in the generation of chloride currents activated during regulatory volume decrease (RVD) after cell swelling (ICl(swell)). Growth factor receptors play a key role in different cellular processes and epidermal growth factor (EGF) regulates swelling-activated chloride permeability.
  
  
  OBJECTIVE
  We set out to investigate if the EGF-induced upregulation of ICl(swell) could be explained by a rearrangement of ICln subcellular distribution and interaction with its molecular partners.
  
  
  METHODS
  NIH-3T3 fibroblasts were serum-deprived for 24 hours and stimulated with EGF (40 ng/ml) for 30 minutes. ICl(swell) activation, ICln distribution and interaction with its molecular partner HSPC038 were assessed by whole cell patch clamp and fluorescence resonance energy transfer (FRET).
  
  
  RESULTS
  EGF treatment significantly enhanced the direct molecular interaction between ICln and HSPC038 and also resulted in an increase of ICln and HSPC038 association with the plasma membrane. Importantly, these events are associated with a significant increase of ICl(swell).
  
  
  CONCLUSIONS
  The present data indicate that EGF might exert its role in the modulation of volume-sensitive chloride currents in part through activation and translocation of ICln and HSPC038 to the plasma membrane.
authors.affiliation: Department of General and Environmental Physiology, University of Bari, Bari.
authors.forename: Grazia
authors.initials: G
authors.lastname: Tamma
authors.forename: Silvia
authors.initials: S
authors.lastname: Dossena
authors.forename: Charity
authors.initials: C
authors.lastname: Nofziger
authors.forename: Giovanna
authors.initials: G
authors.lastname: Valenti
authors.forename: Maria
authors.initials: M
authors.lastname: Svelto
authors.forename: Markus
authors.initials: M
authors.lastname: Paulmichl
mesh_terms: D000818:Animals; D048429:Cell Size; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D018408:Patch-Clamp Techniques; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018118:Chloride Channels; D016895:Culture Media, Serum-Free; D011506:Proteins; D011993:Recombinant Fusion Proteins; C569295:ZNF706 protein, human; D004815:Epidermal Growth Factor