title: Upregulation of BAD, a pro-apoptotic protein of the BCL2 family, in vascular smooth muscle cells exposed to uremic conditions.
abstract: Chronic kidney disease (CKD) has recently emerged as a major risk factor for cardiovascular pathology. CKD patients display accelerated atherosclerotic process, leading to circulatory complications. However, it is currently not clear how uremic conditions accelerate atherosclerosis. Apoptosis is an important homeostatic regulator of vascular smooth cells under pathological conditions. In the present study, we explored the regulation of apoptosis in cells of the vascular wall in the uremic context. We analysed the expression and regulation of the proteins of the BCL2 family that play an essential role in apoptosis. Our results, obtained in mice and primary human smooth muscle cells exposed to two uremic toxins, point to the existence of an alteration in expression and function of one pro-apoptotic member of this family, the protein BAD. We explore the regulation of BAD by uremic toxins and report the sensitization of vascular smooth muscle cells to apoptosis upon BAD induction.
authors.affiliation: Inserm U1088, Faculté de Médecine, Université de Picardie Jules Verne, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Trécherel
authors.forename: Corinne
authors.initials: C
authors.lastname: Godin
authors.forename: Christophe
authors.initials: C
authors.lastname: Louandre
authors.forename: Joyce
authors.initials: J
authors.lastname: Benchitrit
authors.forename: Sabrina
authors.initials: S
authors.lastname: Poirot
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Mazière
authors.forename: Ziad A
authors.initials: ZA
authors.lastname: Massy
authors.forename: Antoine
authors.initials: A
authors.lastname: Galmiche
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003401:Creatine / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014508:Urea / Q000378:metabolism / Q000633:toxicity; D014511:Uremia / Q000378:metabolism* / Q000473:pathology; D051025:bcl-Associated Death Protein / Q000096:biosynthesis*
chemical_list: C494819:BAD protein, human; C494820:Bad protein, mouse; D051025:bcl-Associated Death Protein; D014508:Urea; D003401:Creatine