title: Characterization of synthesized silver nanoparticles and assessment of its genotoxicity potentials using the alkaline comet assay.
abstract: Nano-silver (Nano-Ag) particles were synthesized and then characterized using transmission electron microscopy (TEM) and X-ray diffractometry. TEM showed that Nano-Ag were spherical in shape and their size ranged from 40 to 60nm. X-ray diffractometry indicated that the sample was crystalline and had a face centered cubic structure of pure silver. Genotoxicity of this Nano-Ag was evaluated in human peripheral blood cells using the alkaline comet assay. Results indicated that Nano-Ag (50 and 100μg/mL) caused DNA damage following a 3h treatment. Subsequently, a short treatment of 5min also showed DNA damage. In conclusion, we have shown that the synthesized Nano-Ag induced DNA damage in human peripheral blood cells as detected by the alkaline comet assay. Results further indicated that treatment of cells with Nano-Ag in the presence of hydrogen peroxide did not induce any DNA damage.
authors.affiliation: Nanotechnology Research Center, SRM University, Chennai, India.
authors.forename: Natesan A L
authors.initials: NA
authors.lastname: Flower
authors.forename: Balusamy
authors.initials: B
authors.lastname: Brabu
authors.forename: Murugan
authors.initials: M
authors.lastname: Revathy
authors.forename: Chandrasekaran
authors.initials: C
authors.lastname: Gopalakrishnan
authors.forename: Savarimuthu V K
authors.initials: SV
authors.lastname: Raja
authors.forename: Siva S
authors.initials: SS
authors.lastname: Murugan
authors.forename: Tirukalikundram S
authors.initials: TS
authors.lastname: Kumaravel
mesh_terms: D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010316:Particle Size; D012834:Silver
chemical_list: D012834:Silver; D006861:Hydrogen Peroxide