title: Pellet digestion: a simple and efficient sample preparation technique for LC-MS/MS quantification of large therapeutic proteins in plasma.
abstract: BACKGROUND
  There is a need for a simple and efficient sample preparation technique for LC-MS/MS quantification of large therapeutic proteins in plasma.
  
  
  RESULTS
  The sample preparation technique presented here is based upon trypsin digestion of the pellet obtained following precipitation of the protein analyte from plasma. The pellet digestion technique was shown to facilitate efficient digestion of large therapeutic proteins, with concomitant removal of a substantial amount of potentially problematic plasma phospholipids. The technique was successfully applied to a pharmacokinetic study of a large therapeutic protein.
  
  
  CONCLUSIONS
  This simple sample preparation approach will be beneficial to bioanalytical laboratories engaged in the LC-MS/MS quantification of large therapeutic proteins in biological matrices.
authors.affiliation: Bristol-Myers Squibb, Research & Development, Bioanalytical & Discovery Analytical Sciences, Route 206 & Province Line Road, Princeton, NJ 08543, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ouyang
authors.forename: Michael T
authors.initials: MT
authors.lastname: Furlong
authors.forename: Steven
authors.initials: S
authors.lastname: Wu
authors.forename: Bogdan
authors.initials: B
authors.lastname: Sleczka
authors.forename: James
authors.initials: J
authors.lastname: Tamura
authors.forename: Haiqing
authors.initials: H
authors.lastname: Wang
authors.forename: Suzanne
authors.initials: S
authors.lastname: Suchard
authors.forename: Anish
authors.initials: A
authors.lastname: Suri
authors.forename: Timothy
authors.initials: T
authors.lastname: Olah
authors.forename: Adrienne
authors.initials: A
authors.lastname: Tymiak
authors.forename: Mohammed
authors.initials: M
authors.lastname: Jemal
mesh_terms: D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D059748:Proteolysis; D013048:Specimen Handling / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001798:Blood Proteins