title: [Incidence of alginate-coding gene in carbapenem-resistant Pseudomonas aeruginosa strains].
abstract: Pseudomonas aeruginosa rods are one of the most common isolated opportunistic nosocomial pathogens. Strains usually are capable to secret a capsule-like polysaccharide called alginate important for evasion of host defenses, especially during chronic pulmonary disease of patients with cystic fibrosis. Most genes for alginate biosynthesis and lysis are encoded by the operon. The aim of our study was to evaluate the incidence of algD sequence, generally use for alginate-coding gene detection, in 120 P. aeruginosa strains resistant to carbapenems. All isolates were obtained in the Department of Clinical Microbiology University Hospital no. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz Nicolaus Copernicus University in Toruń. Examined strains demonstrated resistance to carbenicillin (90,0%), ticarcillin (89,2%) and ticarcillin clavulanate (86,7%). All strains were susceptible to colistin. The majority of examined strains was susceptible to ceftazidime and cefepime (40,8% each) and norfloxacin (37,5%). Presence of algD gene - noted in 112 (93,3%) strains proves that not every strain is capable to produce alginate. It was also found out that differences in algD genes incidence in case of different clinical material that strains were isolated from were not statistically important.
authors.affiliation: Katedra i Zakład Mikrobiologii, Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bogiel
authors.forename: Joanna
authors.initials: J
authors.lastname: Kwiecińska-Piróg
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kozuszko
authors.forename: Eugenia
authors.initials: E
authors.lastname: Gospodarek
mesh_terms: D000464:Alginates; D015780:Carbapenems / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D013045:Species Specificity
chemical_list: D000464:Alginates; D015780:Carbapenems; D006603:Hexuronic Acids; D020723:Glucuronic Acid