title: Inter and intra subpopulation genetic variability of roe deer (Capreolus capreolus L.) assessed by I and II class genetic markers.
abstract: The material was collected in three regions of Poland and consisted of 105 randomly chosen individuals killed during hunts (49 males, 56 females), out of which 51 were from Wielkopolska, 22 from Podkarpacie and 32 from Warmia. From each animal a blood sample was taken from the chest, stored in a probe with K2EDTA and frozen. The serum was used to establish the genotype for transferin and albumin whereas the samples with erythrocytes provided information on hemoglobin genotype. DNA was isolated from samples from each individual. Characteristics of eight (from among twelve studied) microsatellite loci and genetic distances were estimated by the use of standard computer package programs. Generally, monomorphism in blood proteins was registered. For the microsatellite loci the number of alleles ranged from 3 in the RT27-6-Fa locus (effectively two as the third allele was present only in two subpopulations with a very low frequency) to 10 in RT1-VI. Five loci showed heterozygosity of 0.5 or above which suggests their usefulness in parentage control. Considerable genetic distances (corresponding to geographical mileages) between the subpopulations were observed based on microsatellite markers.
authors.affiliation: Department of Hunting and Forest Protection, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland. roberto@up.poznan.pl
authors.forename: Robert
authors.initials: R
authors.lastname: Kamieniarz
authors.forename: Anna
authors.initials: A
authors.lastname: Wolc
authors.forename: Mirosław
authors.initials: M
authors.lastname: Lisowski
authors.forename: Mirosława
authors.initials: M
authors.lastname: Dabert
authors.forename: Bartosz
authors.initials: B
authors.lastname: Grajewski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Steppa
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szwaczkowski
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003670:Deer / Q000235:genetics*; D004592:Electrophoresis, Starch Gel; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics
chemical_list: D017931:DNA Primers; D005819:Genetic Markers