title: Therapeutic potential of canine bone marrow stromal cells (BMSCs) in the carbon tetrachloride (CCl4) induced chronic liver dysfunction mouse model.
abstract: Regenerative medicine using bone marrow cells is an attractive therapy for the cure of patients with severe liver disease. Here, we show the therapeutic potential of canine bone marrow stromal cells (BMSCs) in mouse models of CCl(4)-induced chronic liver dysfunction. We used two different models for xenotransplantation, nude mice and cyclosporine A (CSA) immunosuppressed mice. Serum parameters from a standard liver panel were not improved following transplantation. However, fibrotic liver lesions with severe inflammation were decreased in CCl(4)-treated CSA mice following BMSC transplantation. Effective migration of transplanted canine BMSCs was limited to persistently injured liver in CCl(4)-treated CSA mice, where they may be effective in resolving inflammatory fibrotic lesions. These results suggest that canine BMSCs are an effective cell source for liver regeneration.
authors.affiliation: Department of Veterinary Surgery Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Tani
authors.forename: Ryo
authors.initials: R
authors.lastname: Takagishi
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Oda
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Itamoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Shuji
authors.initials: S
authors.lastname: Terai
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Yasuho
authors.initials: Y
authors.lastname: Taura
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056487:Chemical and Drug Induced Liver Injury, Chronic / Q000378:metabolism / Q000628:therapy*; D016572:Cyclosporine / Q000633:toxicity; D004285:Dogs; D016867:Immunocompromised Host; D007166:Immunosuppressive Agents / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D017154:Stromal Cells / Q000502:physiology*; D014998:Y Chromosome / Q000235:genetics
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine; D002251:Carbon Tetrachloride