title: Accuracy profile validation of a new method for carbon monoxide measurement in the human blood using headspace-gas chromatography-mass spectrometry (HS-GC-MS).
abstract: The aim of our study was to provide an innovative headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable for the routine determination of blood CO concentration in forensic toxicology laboratories. The main drawback of the GC/MS methods discussed in literature for CO measurement is the absence of a specific CO internal standard necessary for performing quantification. Even if stable isotope of CO is commercially available in the gaseous state, it is essential to develop a safer method to limit the manipulation of gaseous CO and to precisely control the injected amount of CO for spiking and calibration. To avoid the manipulation of a stable isotope-labeled gas, we have chosen to generate in a vial in situ, an internal labeled standard gas ((13)CO) formed by the reaction of labeled formic acid formic acid (H(13)COOH) with sulfuric acid. As sulfuric acid can also be employed to liberate the CO reagent from whole blood, the procedure allows for the liberation of CO simultaneously with the generation of (13)CO. This method allows for precise measurement of blood CO concentrations from a small amount of blood (10 μL). Finally, this method was applied to measure the CO concentration of intoxicated human blood samples from autopsies.
authors.affiliation: Forensic Chemistry and Toxicology Unit, University Center of Legal Medicine Lausanne-Geneva, Lausanne, Switzerland. vincent.varlet@chuv.ch
authors.forename: V
authors.initials: V
authors.lastname: Varlet
authors.forename: E Lagroy
authors.initials: EL
authors.lastname: De Croutte
authors.forename: M
authors.initials: M
authors.lastname: Augsburger
authors.forename: P
authors.initials: P
authors.lastname: Mangin
mesh_terms: D001344:Autopsy; D002248:Carbon Monoxide / Q000097:blood* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D002248:Carbon Monoxide