title: Endothelin-1 and type III collagen alterations in the heart of rats             following cisplatin-induced toxicity.
abstract: Cisplatin-induced heart damage is associated with enzymes and protein alterations. The purpose of this study was to investigate cisplatin-induced alterations in cardiac endothelin (ET)-1 and type III collagen following administration of cisplatin. Male Wistar rats were randomly divided into two groups: a control and a cisplatin group. The cisplatin group received cisplatin intraperitoneally (i.p.) at a single dose of 7 mg/kg on day 6, while the controls were given the same amount of saline via the same route. On day 11, the rats were anesthetized and a thoracotomy was performed on all animals. Immunohistochemical analysis was performed to evaluate the protein expression of ET-1 and type III collagen. Sections were analyzed by digital image analysis. Results showed that the cardiac protein expression of ET-1 and type III collagen was altered following cisplatin-induced toxicity in rats. The expression of ET-1 and type III collagen was significantly increased after cisplatin treatment, supported by integrated optical density, when compared to the control group (P<0.05). The present findings indicate that such alterations may be reflected in abnormal cardiac function. Additionally, the proteins identified in this study may benefit investigations into the mechanisms underlying cisplatin-induced toxicity, thereby providing the necessary evidence for further research.
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Dali University, Dali, Yunnan 671000, PR China. hqy0726@126.com
authors.forename: Quan-Yong
authors.initials: QY
authors.lastname: Huang
authors.forename: Xue-Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D024061:Collagen Type III / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D024061:Collagen Type III; D019332:Endothelin-1; D002945:Cisplatin