title: Differential expression of iron metabolism proteins in diabetic and diabetic iron-supplemented rat liver.
abstract: Diabetes mellitus is associated with altered iron homeostasis that can potentially effect reactive oxygen species generation and contribute to diabetes-related complications. We investigated, by quantitative polymerase chain reaction, whether the expression of liver hepcidin, ferritin, and TfR-1 is altered in diabetes. Rats in the control (C) group received a standard diet; control iron (CI) group received a standard diet supplemented with iron; diabetic (D) group received an injection of streptozotocin; and diabetic iron (DI) group received streptozotocin and the diet with iron. Animals of the D group showed higher levels of serum iron, increased concentration of carbonyl protein, and a decrease in antioxidant status. Group D rats showed increased hepatic expression of Trf-1 compared to the other groups. Iron supplementation reversed this increase. Hepcidin mRNA was 81% higher in DI than in C and CI rats. The results suggest that diabetes, with or without excess iron, can cause perturbations in iron status, hepcidin and Trf-1 expression.
authors.affiliation: Programa de Pós graduação em Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil. maisasilv@yahoo.com.br
authors.forename: Maísa
authors.initials: M
authors.lastname: Silva
authors.forename: Cintia Lopes
authors.initials: CL
authors.lastname: de Brito Magalhães
authors.forename: Riva
authors.initials: R
authors.lastname: de Paula Oliveira
authors.forename: Marcelo Eustáquio
authors.initials: ME
authors.lastname: Silva
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Pedrosa
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D019587:Dietary Supplements; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D064451:Hepcidins; D007501:Iron / Q000008:administration & dosage* / Q000493:pharmacokinetics; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344; D060888:Real-Time Polymerase Chain Reaction; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D023181:Antimicrobial Cationic Peptides; D000975:Antioxidants; D001786:Blood Glucose; C578703:Hamp protein, rat; D064451:Hepcidins; D011990:Receptors, Transferrin; C545564:Tfrc protein, rat; D005293:Ferritins; D007501:Iron