title: Removal of monomer delignification products by laccase from Trametes versicolor.
abstract: The influence of a laccase from Trametes versicolor on the removal of phenolic monomers in liquid hot water pretreated wheat straw supernatants (LHW-S) was examined. Beside the total phenol content derived by Folin-Ciocalteu (FC-) assay, phenolic monomers were measured via headspace-solid phase micro-extraction (HS-SPME)/GC-MS. A notable decrease of the phenols was achieved using 0.2 and 0.5 U/mL laccase whilst higher dosage showed no improvement. Nearly all kind of monomer phenolic compounds identified in the LHW-S were found to be removed after 24h. However, acetophenone and 4-hydroxybenzaldehyde (HBA) were obviously not affected by laccase. Summarizing, three laccase reaction groups (LRG) of phenolic monomers could be classified: immediate removal (LRG-A), degradation after 1 day (LRG-B), no effect of laccase (LRG-C). Additionally, HS-SPME/GC was found to be a powerful tool to study the reaction of laccase and phenolic monomers in complex lignocellulose derived solutions.
authors.affiliation: Institute of Chemistry of Biogenic Resources, Technische Universität München, Schulgasse 16, 94315 Straubing, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Kolb
authors.forename: Volker
authors.initials: V
authors.lastname: Sieber
authors.forename: Manfred
authors.initials: M
authors.lastname: Amann
authors.forename: Martin
authors.initials: M
authors.lastname: Faulstich
authors.forename: Doris
authors.initials: D
authors.lastname: Schieder
mesh_terms: D006358:Hot Temperature; D042845:Laccase / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry*; D055454:Trametes / Q000201:enzymology*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010636:Phenols; D008031:Lignin; D042845:Laccase