title: Polymeric cyclosporine-A nanoparticles for ocular application.
abstract: In the present study, cyclosporine A (CsA) was incorporated successfully into cationic Eudragit RS 100 nanoparticles (EPNs) aiming ocular application. Physicochemical characterization of the EPNs prepared was performed during the storage period of 6 months. Following in vitro release tests, sheep were used in in vivo studies where 100 microL of formulation was applied to both eyes (n = 6) under veterinarian supervision. Aqueous and vitreous humour samples were collected at predetermined time intervals and analyzed by enzyme immune assay (EIA). In vitro relase studies showed the extended release of the incorporated drug from the nanoparticles. However in vivo results demonstrated the prolonged residence time of CsA in the deeper layers (vitreous humour) of the eye with positively charged EPNs.
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Technology, Anadolu University, Eskişehir 26470, Türkiye
authors.forename: Ebru
authors.initials: E
authors.lastname: Başaran
authors.forename: Müzeyyen
authors.initials: M
authors.lastname: Demirel
authors.forename: Başar
authors.initials: B
authors.lastname: Sirmagül
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yazan
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000494:pharmacology*; D005123:Eye / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012756:Sheep; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D014961:X-Ray Diffraction
chemical_list: D000180:Acrylic Resins; D011108:Polymers; C050528:Eudragit RS; D016572:Cyclosporine