title: Comparison of oxidative stress and  the frequency of polymorphisms in the HFE  gene between hemoglobin S trait blood donors and sickle cell disease patients.
abstract: It is  well documented that Hb S and iron affect blood cells, and trigger oxidative  processes and generation of free radicals with potential for lipid  peroxidation. We evaluated the frequency of polymorphisms in the HFE gene  in Hb AS blood donors and how these polymorphisms influenced lipid peroxidation  and antioxidant capacity. Blood samples were collected from 211 Hb AS blood  donors, 119 Hb AA blood donors as a control group, and 28 sickle cell disease  patients (Hb SS). The H63D allele was found at a frequency of 10.5% in the Hb AS  samples, and the C282Y allele frequency was 0.7%. In the control group, the  frequencies of the H63D and C282Y alleles were 13.4 and 2.1%, respectively. In  the sickle-cell disease patients, the H63D and the C282Y allele frequencies  were 10.7 and 3.5%, respectively. The frequencies of the C282Y and H63D  polymorphisms in Hb AS blood donors are similar to those reported for the  Brazilian population. Serum malondialdehyde values, indicative of lipid peroxidation,  were highest in sickle cell patients, independent of the polymorphisms in the HFE gene, with significant differences, showing the influence of Hb S allele in  the levels of lipid peroxidation. However, the trolox equivalent antioxidant  capacity average levels, indicative of the antioxidant capacity, were reduced  with significant differences, indicating that in spite of a lipid peroxidation  raise, this is not followed by the increased of the antioxidant capacity,  leading to oxidative stress.
authors.affiliation: Laboratório de Hemoglobinas e Genética das Doenças Hematológicas, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual de São Paulo "Julio de Mesquita Filho, São José do Rio Preto, SP, Brasil. libaracioli@yahoo.com.br
authors.forename: L M S
authors.initials: LM
authors.lastname: Viana-Baracioli
authors.forename: N C
authors.initials: NC
authors.lastname: Tukamoto Junior
authors.forename: O
authors.initials: O
authors.lastname: Ricci Junior
authors.forename: L C
authors.initials: LC
authors.lastname: Mattos
authors.forename: I L
authors.initials: IL
authors.lastname: Ângulo
authors.forename: C R
authors.initials: CR
authors.lastname: Bonini-Domingos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism; D001782:Blood Donors; D002648:Child; D002839:Chromans / Q000378:metabolism; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D000071020:Hemochromatosis Protein; D006451:Hemoglobin, Sickle / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic; D012727:Sex Characteristics; D012805:Sickle Cell Trait / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D002839:Chromans; C103673:HFE protein, human; D000071020:Hemochromatosis Protein; D006451:Hemoglobin, Sickle; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D008315:Malondialdehyde; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid