title: Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.
abstract: A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.
authors.affiliation: Department of Environmental Sciences, University of California, Riverside, CA, USA .
authors.forename: Daniela
authors.initials: D
authors.lastname: Dunams
authors.forename: Payal
authors.initials: P
authors.lastname: Sarkar
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
authors.forename: Marylynn V
authors.initials: MV
authors.lastname: Yates
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D002460:Cell Line; D004457:Echovirus Infections / Q000175:diagnosis* / Q000821:virology; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D013997:Time Factors; D014773:Virology / Q000379:methods*; D014779:Virus Replication