title: [Application of multiplex nested RT-PCR for fast detection of PDGFRα fusion gene in myeloproliferative neoplasms].
abstract: This study was aimed to explore the applicable value of multiplex nested reverse transcription-polymerase chain reaction (multiplex nested RT-PCR)for the detection of platelet-derived growth factor receptor alpha (PDGFRα) fusion gene in myeloproliferative neoplasms (MPN). Bone marrow or peripheral blood samples from 146 patients with MPN were analyzed by using a novel multiplex nested RT-PCR. The result showed that PDGFRα fusion gene was found in 6 out of the 146 bone marrow or peripheral blood samples, the positive rate was 4.11%, 4 from the 6 patients received treatment with imatinib and showed therapeutic effect. It is concluded that the multiplex nested RT-PCR has a series of advantages such as high sensitivity, specificity, and time-saving, and can be applied for determination of the molecular type of MPN, and also for the diagnosis and therapy of MPN.
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Jiang
authors.forename: Min-Hang
authors.initials: MH
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jing
authors.forename: Quan-Shun
authors.initials: QS
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D019046:Bone Marrow Neoplasms / Q000175:diagnosis / Q000235:genetics; D050939:Gene Fusion; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D009196:Myeloproliferative Disorders / Q000175:diagnosis / Q000235:genetics*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D020796:Receptor, Platelet-Derived Growth Factor alpha