title: An in vivo and in vitro analysis of free radical scavenging potential possessed by Desmodium gangeticum chloroform root extract: interpretation by gsms.
abstract: In the present study, the effect of DG chloroform root extract was assessed on isolated rat heart and in-vitro antioxidant models. Ischemia reperfusion injury was experimentally induced by using Langendroff apparatus. The free radical scavenging potential was studied in vitro by using different antioxidant models such as DPPH, super oxide scavenging activity, hydroxide scavenging activity and nitric oxide scavenging activity. Both experimental approaches not only substantiate its antioxidant potential but also the cardio-protection imparted by the extract. The cardio-stimulatory effects were investigated for the extract by treating it as a pre-conditioning agent against myocardial ischemia reperfusion injury. The improved antioxidant status of the myocardium indirectly predicts reduced oxidative stress mediated by ischemic reperfusion with evident reduction of infarct size determined by cardiac marker protein. These findings indicate that DG chloroform root extract may possess therapeutic potential against ischemia reperfusion injury.
authors.affiliation: Department of Biotechnology, SASTRA University, Thirumalaisamudram, India.
authors.forename: Shyam
authors.initials: S
authors.lastname: Srivats
authors.forename: Gomathi
authors.initials: G
authors.lastname: Ramakrishnan
authors.forename: Jose
authors.initials: J
authors.lastname: Paddikkala
authors.forename: Gino Andrew
authors.initials: GA
authors.lastname: Kurian
mesh_terms: D000818:Animals; D002725:Chloroform / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007887:Fabaceae; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C521776:Desmodium gangeticum extract; D016166:Free Radical Scavengers; D010936:Plant Extracts; D002725:Chloroform