title: Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8.
abstract: The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while CPA-2 viability was less than 2%. CPA-8 survival directly related with its ability to produce endospores. Spray-dried CPA-8 reduced Monilinia fructicola conidia germination similarly to fresh cells, demonstrating that spray-drying did not adversely affect biocontrol efficacy. Endospore production thus improves CPA-8 resistance to spray-drying. These results can provide a reliable basis for optimising of the spray-drying formulation process for CPA-8 and other microorganisms.
authors.affiliation: IRTA, XaRTA-Post-harvest, 191 Rovira Roure, 25198, Lleida, Catalonia, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Yánez-Mendizabal
authors.forename: I
authors.initials: I
authors.lastname: Viñas
authors.forename: J
authors.initials: J
authors.lastname: Usall
authors.forename: T
authors.initials: T
authors.lastname: Cañamás
authors.forename: N
authors.initials: N
authors.lastname: Teixidó
mesh_terms: D000336:Aerosols; D001203:Ascomycota / Q000254:growth & development; D001412:Bacillus subtilis / Q000502:physiology*; D003890:Desiccation; D056265:Microbial Interactions; D050296:Microbial Viability; D010572:Pest Control, Biological / Q000379:methods*; D013171:Spores, Bacterial / Q000502:physiology*; D013172:Spores, Fungal / Q000254:growth & development; D013997:Time Factors
chemical_list: D000336:Aerosols