title: Assessment of paraoxonase 1, xanthine oxidase and glutathione peroxidase activities, nitric oxide and thiol levels in women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To investigate whether there is any relation between oxidative stress and the antioxidant system in the development of polycystic ovary syndrome (PCOS) by measuring serum nitric oxide (NO) levels and xanthine oxidase (XO) activity (a generator of reactive oxygen species) and antioxidant status by measuring serum thiol levels and glutathione peroxidase (GSHPx) and paraoxonase 1 (PON1) activities.
  
  
  METHODS
  Prospective case-control study.
  
  
  METHODS
  University hospital in Turkey.
  
  
  METHODS
  Thirty women with polycystic ovary syndrome and 20 age- and sex-matched healthy control subjects were included.
  
  
  METHODS
  Serum XO, PON1 and GSHPx activity and NO and thiol levels were determined by spectrophotometric methods.
  
  
  METHODS
  Activity of serum XO, PON1 and GSH, as well as NO and thiol levels.
  
  
  RESULTS
  Serum XO activities were higher in women with PCOS than in the control women (p<0.001). The PON1 activity was lower in women with PCOS than in the control women (p<0.001). No significant difference was found between NO and thiol levels and GSHPx activities of women with PCOS and the control women (p>0.05). Serum PON1 activities were negatively correlated with serum XO activities and NO levels.
  
  
  CONCLUSIONS
  Increased oxidant XO activity and decreased lipid antioxidant PON1 activity, along with the observed negative correlation between these parameters, suggests that women with PCOS are under oxidative stress and that there is XO-mediated lipid peroxidation, which may be related to increased atherosclerosis seen in later life in such women.
authors.affiliation: Departments of Biochemistry and Clinical Biochemistry Obstetrics, Erciyes University Faculty of Medicine, Kayseri, Turkey. gbaskol@erciyes.edu.tr
authors.forename: Gulden
authors.initials: G
authors.lastname: Baskol
authors.forename: Ercan
authors.initials: E
authors.lastname: Aygen
authors.forename: Feray
authors.initials: F
authors.lastname: Erdem
authors.forename: Ayşen
authors.initials: A
authors.lastname: Caniklioğlu
authors.forename: Figen
authors.initials: F
authors.lastname: Narin
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Sahin
authors.forename: Tugba
authors.initials: T
authors.lastname: Kaya
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000201:enzymology; D011446:Prospective Studies; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000097:blood*; D014969:Xanthine Oxidase / Q000097:blood*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human