title: Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?
abstract: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortium, refining our previous observations of aerobic degradation. The aerobic consortium depleted, in decreasing order, hydrocarbons > hopanes > steranes > tricyclic terpanes while the anaerobic consortium depleted hydrocarbons > steranes > hopanes > tricyclic terpanes. The oxygen content of the mixed consortia was measured from time to time revealing alternating periods of microaerobicity (O2 ~0.8 mg.L-1) and of aerobicity (O2~6.0 mg.L-1). In this experiment, the petroleum biodegradation changed from time to time, alternating periods of biodegradation similar to the aerobic process and periods of biodegradation similar to the anaerobic process. The consortia showed preferences for metabolizing hydrocarbons > hopanes > steranes > tricyclic terpanes during a 90-day period, after which this trend changed and steranes were more biodegraded than hopanes. The analysis of aerobic oil degrading microbiota by the 16S rRNA gene clone library detected the presence of Bacillus, Brevibacterium, Mesorhizobium and Achromobacter, and the analysis of the anaerobic oil degrading microbiota using the same technique detected the presence of Bacillus and Acinetobacter (facultative strains). In the mixed consortia Stenotrophomonas, Brevibacterium, Bacillus, Rhizobium, Achromobacter and 5% uncultured bacteria were detected. This is certainly a new contribution to the study of reservoir biodegradation processes, combining two of the more important accepted hypotheses.
authors.affiliation: Chemistry Institute, University of Campinas - UNICAMP, POB 6154, 13084-971 Campinas, SP, Brazil. anita@iqm.unicamp.br.
authors.forename: Georgiana F
authors.initials: GF
authors.lastname: da Cruz
authors.forename: Suzan P
authors.initials: SP
authors.lastname: de Vasconcellos
authors.forename: Célio Ff
authors.initials: CF
authors.lastname: Angolini
authors.forename: Bruna M
authors.initials: BM
authors.lastname: Dellagnezze
authors.forename: Isabel Ns
authors.initials: IN
authors.lastname: Garcia
authors.forename: Valéria M
authors.initials: VM
authors.lastname: de Oliveira
authors.forename: Eugenio V
authors.initials: EV
authors.lastname: Dos Santos Neto
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Marsaioli