title: The effect of base-pair sequence on electrochemically driven denaturation.
abstract: Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, E(m)) directly correlates with the calculated nearest neighbour and experimental melting temperatures; T(m), for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.
authors.affiliation: School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Johnson
authors.forename: Rachel
authors.initials: R
authors.lastname: Gao
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
authors.forename: Philip N
authors.initials: PN
authors.lastname: Bartlett
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D015195:Drug Design; D055664:Electrochemical Techniques; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation
chemical_list: D015342:DNA Probes; D004247:DNA