title: Development of an orange juice surrogate for the study of dental erosion.
abstract: The aim of this study was to create a synthetic juice (SJ) to be used as a surrogate for natural orange juices in erosion studies, verifying its erosive potential. The SJ was formulated based on the chemical composition of orange juices from different locations. Forty enamel and 40 root dentin specimens were randomly assigned into 4 experimental groups (n = 10): SJ; 1% Citric Acid (CA); Minute Maid Original® (MM) and Florida Natural Original® (FN). The specimens were immersed in their respective solutions for 5 min, 6x/day for 5 days, in an erosion-remineralization cycling model. Enamel specimens were analyzed by surface Knoop microhardness and optical profilometry and dentin specimens only by optical profilometry. Outcomes were analyzed statistically by ANOVA followed by Tukey's test considering a significance level of 5%. For enamel, the surface loss and microhardness changes found for MM and SJ groups were similar (p>0.05) and significantly lower (p<0.01) than those found in the CA group. For dentin, CA promoted significantly greater (p<0.01) surface loss compared with all the other groups. No significant difference (p>0.05) was observed in dentin surface loss between MM and SJ. In conclusion, CA was the most erosive solution, and SJ had a similar erosive potential to that of MM natural orange juice.
authors.affiliation: Department of Restorative Dentistry, Dental School, University of São Paulo, São Paulo, Brazil. tais.sca@usp.br
authors.forename: Tais
authors.initials: T
authors.lastname: Scaramucci
authors.forename: Anderson T
authors.initials: AT
authors.lastname: Hara
authors.forename: Domenick T
authors.initials: DT
authors.lastname: Zero
authors.forename: Stella S
authors.initials: SS
authors.lastname: Ferreira
authors.forename: Idalina V
authors.initials: IV
authors.lastname: Aoki
authors.forename: Maria Angela P
authors.initials: MA
authors.lastname: Sobral
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis; D001628:Beverages / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002626:Chemistry, Pharmaceutical; D019343:Citric Acid / Q000032:analysis / Q000494:pharmacology; D032084:Citrus sinensis / Q000737:chemistry; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D005638:Fruit / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D006244:Hardness; D006801:Humans; D008274:Magnesium / Q000032:analysis; D008422:Materials Testing; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D013395:Sucrose / Q000032:analysis; D013997:Time Factors; D014077:Tooth Erosion / Q000209:etiology*; D014089:Tooth Remineralization
chemical_list: D010758:Phosphorus; D019343:Citric Acid; D013395:Sucrose; D008274:Magnesium; D005947:Glucose; D001205:Ascorbic Acid; D011188:Potassium; D002118:Calcium