title: QTL mapping of physiological traits associated with salt tolerance in Medicago truncatula Recombinant Inbred Lines.
abstract: In this study, QTL mapping of physiological traits in the model Legume (Medicago truncatula) was performed using a set of RILs derived from LR5. Twelve parameters associated with Na+ and K+ content in leaves, stems and roots were measured. Broad-sense heritability of these traits was ranged from 0.15 to 0.83 in control and from 0.14 to 0.61 in salt stress. Variation among RILs was dependent on line, treatment and line by treatment effect. We mapped 6 QTLs in control, 2 in salt stress and 5 for sensitivity index. No major QTL was identified indicating that tolerance to salt stress is governed by several genes with low effects. Detected QTL for leaf, stem and root traits did not share the same map locations, suggesting that genes controlling transport of Na+ and K+ may be different. The maximum of QTL was observed on chromosome 1, no QTL was detected on chromosomes 5 and 6.
authors.affiliation: Laboratory of Legumes, Centre of Biotechnology of Borj Cedria, B.P. 901, 2050 Hammam-Lif, Tunisia. bio.soumaya@gmail.com
authors.forename: Soumaya
authors.initials: S
authors.lastname: Arraouadi
authors.forename: Mounawer
authors.initials: M
authors.lastname: Badri
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.forename: Thierry
authors.initials: T
authors.lastname: Huguet
authors.forename: Mohamed Elarbi
authors.initials: ME
authors.lastname: Aouani
mesh_terms: D000704:Analysis of Variance; D002874:Chromosome Mapping; D017343:Genes, Plant; D007178:Inbreeding; D046913:Medicago truncatula / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D040641:Quantitative Trait Loci; D055049:Salt Tolerance / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D012964:Sodium; D011188:Potassium