title: Amyloid precursor protein binding protein-1 knockdown reduces neuronal differentiation in fetal neural stem cells.
abstract: Amyloid precursor protein binding protein-1 (APP-BP1) was first identified as an interacting protein of APP. In this study, we explored whether APP-BP1 plays a role in neuronal differentiation of fetal neural stem cells. APP-BP1 knockdown by small interfering RNA treatment was found to downregulate neuronal differentiation and to upregulate APP intracellular domain production from APP in fetal neural stem cells. Furthermore, the change in gene expression profiles was systemically examined by DNA microarray. The expression of several genes including ephrin A2 was upregulated by APP-BP1 knockdown as assessed with DNA microarray and reverse transcriptase-polymerase chain reaction. Taken together, our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells.
authors.affiliation: Department of Pharmacology, Seoul National University, College of Medicine, Seoul, Korea.
authors.forename: Bo-Hyun
authors.initials: BH
authors.lastname: Hong
authors.forename: Sungji
authors.initials: S
authors.lastname: Ha
authors.forename: Yuyoung
authors.initials: Y
authors.lastname: Joo
authors.forename: Jeong A
authors.initials: JA
authors.lastname: Kim
authors.forename: Keun-A
authors.initials: KA
authors.lastname: Chang
authors.forename: Ran-Sook
authors.initials: RS
authors.lastname: Woo
authors.forename: Yoo-Hun
authors.initials: YH
authors.lastname: Suh
authors.forename: Joung-Hun
authors.initials: JH
authors.lastname: Kim
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Kim
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D053686:Fetal Stem Cells / Q000166:cytology*; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D058953:Neural Stem Cells / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044764:Ubiquitin-Activating Enzymes
chemical_list: D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D044764:Ubiquitin-Activating Enzymes; C491802:NAE protein, human