title: Localization of Magic-F1 transgene, involved in muscular hypertrophy, during early myogenesis.
abstract: We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5 dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5-10.5-11.5 dpc) and cryostat sections for later stages (11.5-13.5-15.5-17.5 dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.
authors.affiliation: Human Anatomy Section, University of Pavia, Via Forlanini 8, 27100 Pavia, Italy.
authors.forename: Flavio
authors.initials: F
authors.lastname: Ronzoni
authors.forename: Matilde
authors.initials: M
authors.lastname: Bongio
authors.forename: Silvio
authors.initials: S
authors.lastname: Conte
authors.forename: Luigi
authors.initials: L
authors.lastname: Vercesi
authors.forename: Marco
authors.initials: M
authors.lastname: Cassano
authors.forename: Carla
authors.initials: C
authors.lastname: Tribioli
authors.forename: Daniela
authors.initials: D
authors.lastname: Galli
authors.forename: Riccardo
authors.initials: R
authors.lastname: Bellazzi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Magenes
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D020869:Gene Expression Profiling; D006984:Hypertrophy / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000196:embryology / Q000473:pathology*; D009928:Organ Specificity; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D011994:Recombinant Proteins; C533045:magic-factor 1 protein, mouse; C068686:Pax3 protein, mouse