title: Self-complementary AAVs induce more potent transgene product-specific immune responses compared to a single-stranded genome.
abstract: Using a mouse model we show that self-complementary (sc) adeno-associated virus (AAV) vectors pseudotyped with capsids of serotypes 2, 7 or 8 induce more potent transgene product-specific CD8(+) T cell and antibody responses compared to corresponding single-stranded (ss)AAV vectors. These data suggest that the higher and more rapidly appearing amounts of transgene product achieved with scAAV vectors may increase detrimental immune responses in gene transfer recipients.
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
authors.forename: TeLang
authors.initials: T
authors.lastname: Wu
authors.forename: Katrin
authors.initials: K
authors.lastname: Töpfer
authors.forename: Shih-Wen
authors.initials: SW
authors.lastname: Lin
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Ang
authors.initials: A
authors.lastname: Bian
authors.forename: Xiang Y
authors.initials: XY
authors.lastname: Zhou
authors.forename: Katherine A
authors.initials: KA
authors.lastname: High
authors.forename: Hildegund C J
authors.initials: HC
authors.lastname: Ertl
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D000229:Dependovirus / Q000235:genetics* / Q000276:immunology; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics* / Q000276:immunology; D016679:Genome, Viral; D016130:Immunophenotyping; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D019076:Transgenes / Q000276:immunology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D054301:gag Gene Products, Human Immunodeficiency Virus