title: Distribution and expression of non-neuronal transient receptor potential (TRPV) ion channels in rosacea.
abstract: Rosacea is a frequent chronic inflammatory skin disease of unknown etiology. Because early rosacea reveals all characteristics of neurogenic inflammation, a central role of sensory nerves in its pathophysiology has been discussed. Neuroinflammatory mediators and their receptors involved in rosacea are poorly defined. Good candidates may be transient receptor potential (TRP) ion channels of vanilloid type (TRPV), which can be activated by many trigger factors of rosacea. Interestingly, TRPV2, TRPV3, and TRPV4 are expressed by both neuronal and non-neuronal cells. Here, we analyzed the expression and distribution of TRPV receptors in the various subtypes of rosacea on non-neuronal cells using immunohistochemistry, morphometry, double immunoflourescence, and quantitative real-time PCR (qRT-PCR) as compared with healthy skin and lupus erythematosus. Our results show that dermal immunolabeling of TRPV2 and TRPV3 and gene expression of TRPV1 is significantly increased in erythematotelangiectatic rosacea (ETR). Papulopustular rosacea (PPR) displayed an enhanced immunoreactivity for TRPV2, TRPV4, and also of TRPV2 gene expression. In phymatous rosacea (PhR)-affected skin, dermal immunostaining of TRPV3 and TRPV4 and gene expression of TRPV1 and TRPV3 was enhanced, whereas epidermal TRPV2 staining was decreased. Thus, dysregulation of TRPV channels also expressed by non-neuronal cells may be critically involved in the initiation and/or development of rosacea. TRP ion channels may be targets for the treatment of rosacea.
authors.affiliation: Department of Dermatology, University Hospital Münster, Münster, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Sulk
authors.forename: Stephan
authors.initials: S
authors.lastname: Seeliger
authors.forename: Jerome
authors.initials: J
authors.lastname: Aubert
authors.forename: Verena D
authors.initials: VD
authors.lastname: Schwab
authors.forename: Ferda
authors.initials: F
authors.lastname: Cevikbas
authors.forename: Michel
authors.initials: M
authors.lastname: Rivier
authors.forename: Pawel
authors.initials: P
authors.lastname: Nowak
authors.forename: Johannes J
authors.initials: JJ
authors.lastname: Voegel
authors.forename: Jörg
authors.initials: J
authors.lastname: Buddenkotte
authors.forename: Martin
authors.initials: M
authors.lastname: Steinhoff
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001706:Biopsy; D016022:Case-Control Studies; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism / Q000473:pathology; D012393:Rosacea / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D050916:TRPV Cation Channels / Q000378:metabolism*; D053802:Tryptases / Q000378:metabolism; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D050916:TRPV Cation Channels; D053802:Tryptases; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C400769:TRPV2 protein, human; C459445:TRPV3 protein, human; C416986:TRPV4 protein, human