title: Global chemical composition and antioxidant and anti-tuberculosis activities of various extracts of Globularia alypum L. (Globulariaceae) leaves.
abstract: In this work, an evaluation of the biological activities of Globularia alypum L. extracts and their global chemical composition was realized. Extracts from G. alypum were obtained by two extraction methods. The composition of polyphenols (8.5-139.95 g gallic acid equivalent/Kg of dry mass), tannins (1.39-18.65 g catechin equivalent/Kg of dry mass), anthocyanins (8.17-70.69 mg cyanidin equivalent/Kg of dry mass) and flavonoids (0.31-19.28 g quercetin equivalent/Kg of dry mass) was evaluated. The samples were subjected to a screening for their antioxidant activities using the DPPH* and ABTS*+ assays. For the first time, the anti-tuberculosis activity (H(37)Rv) for G. alypum was tested against Mycobacterium tuberculosis. The strongest antioxidant activity was obtained for the methanol extract (IC(50 ) = 15.58 ± 0.168 mg/L) and the best anti-tuberculosis activity was obtained for the petroleum ether extract (IC(50 )= 77 mg/L). We have found a positive correlation between the total phenolics content and the antioxidant activity R2 = 0.88 (DPPH*) and R2 = 0.97 (ABTS*+). We have found also a positive correlation between the flavonoid content and the antioxidant activity R2 = 0.91 (DPPH*) and R2 = 0.91 (ABTS*+).
authors.affiliation: Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique UMR CNRS 5623, University of Toulouse, University of Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, France.
authors.forename: Daycem
authors.initials: D
authors.lastname: Khlifi
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.forename: Akrem
authors.initials: A
authors.lastname: El Hayouni
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cazaux
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Souchard
authors.forename: François
authors.initials: F
authors.lastname: Couderc
authors.forename: Jalloul
authors.initials: J
authors.lastname: Bouajila
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000737:chemistry* / Q000494:pharmacology*; D052160:Benzothiazoles / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D028083:Scrophulariaceae / Q000737:chemistry*; D013451:Sulfonic Acids / Q000378:metabolism; D013634:Tannins / Q000032:analysis
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D000995:Antitubercular Agents; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013634:Tannins; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl