title: C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion.
abstract: BACKGROUND
  C57BL/6 and 129/Sv are the 2 most commonly used strains of mice in renal ischemia-reperfusion injury (IRI) studies, yet there are currently no studies that contrast differences in the degree of renal injury after ischemia-reperfusion.
  
  
  METHODS
  To evaluate renal IRI in male C57BL/6 and 129/Sv mice, we performed unilateral clamping of the left renal pedicle for 45 minutes and compared the degree of renal tissue damage and function. To measure function and tissue damage we examined: glomerular filtration rate (GFR; by inulin clearance), renal blood flow (RBF; by p-aminohippurate [PAH] clearance), renal morphology, immunohistochemistry for infiltrating leukocytes, and fibrogenic markers by Sirius red staining.
  
  
  RESULTS
  After unilateral IRI, 129/sv mice had significantly less GFR and RBF disfunction at both day 14 (d14) and d28. 129/sv mice also had significantly less acute tubular necrosis on d1 and fewer infiltrating leukocytes on d28, as well as less collagen deposition on d28 than C57BL/6 mice.
  
  
  CONCLUSIONS
  C57BL/6 mice were much more sensitive to damage caused by renal IRI than are 129/Sv mice.
authors.affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College of HUST, Wuhan, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Lu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Nelli
authors.initials: N
authors.lastname: Shushakova
authors.forename: Roland
authors.initials: R
authors.lastname: Schmitt
authors.forename: Jan
authors.initials: J
authors.lastname: Menne
authors.forename: Nathan
authors.initials: N
authors.lastname: Susnik
authors.forename: Matthias
authors.initials: M
authors.lastname: Meier
authors.forename: Michael
authors.initials: M
authors.lastname: Leitges
authors.forename: Hermann
authors.initials: H
authors.lastname: Haller
authors.forename: Faikah
authors.initials: F
authors.lastname: Gueler
authors.forename: Song
authors.initials: S
authors.lastname: Rong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D005919:Glomerular Filtration Rate; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000473:pathology; D010641:Phenotype; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012720:Severity of Illness Index; D013045:Species Specificity; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III