title: Rad26p regulates the occupancy of histone H2A-H2B dimer at the active genes in vivo.
abstract: Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes is not altered following transcriptional induction in the absence of Rad26p. A histone H2A-H2B dimer-enriched chromatin in Δrad26 is correlated to decreased association of RNA polymerase II with the active coding sequences (and hence transcription). However, the reduced association of RNA polymerase II with the active coding sequence in the absence of Rad26p is not due to the defect in formation of transcription complex at the promoter. Thus, Rad26p regulates the occupancy of histone H2A-H2B dimer, which is correlated to the association of elongating RNA polymerase II with active GAL genes. Similar results are also found at other inducible non-GAL genes. Collectively, our results define a new role of Rad26p in orchestrating chromatin structure and hence transcription in vivo.
authors.affiliation: Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, USA.
authors.forename: Shivani
authors.initials: S
authors.lastname: Malik
authors.forename: Priyasri
authors.initials: P
authors.lastname: Chaurasia
authors.forename: Shweta
authors.initials: S
authors.lastname: Lahudkar
authors.forename: Bhawana
authors.initials: B
authors.lastname: Uprety
authors.forename: Sukesh R
authors.initials: SR
authors.lastname: Bhaumik
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002843:Chromatin / Q000737:chemistry; D019281:Dimerization; D005686:Galactokinase / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006657:Histones / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics; D017434:Protein Structure, Tertiary; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; C518355:GAL10 protein, S cerevisiae; D006657:Histones; D026901:Membrane Transport Proteins; C470555:STL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases; C499333:SNF2 protein, S cerevisiae; C465758:RAD26 protein, S cerevisiae