title: Comparative study of hemagglutination and lectin activity in Australian medicinal mushrooms (higher Basidiomycetes).
abstract: Fifteen Australian mushroom species (higher Basidiomycetes) were assessed for hemagglutination and lectin activity. Hemagglutination activity was evaluated using both neuraminidase treated and untreated rabbit and human A, B, and O erythrocytes. Lectin activity was determined by the ability of various mono- and oligosaccharides to inhibit hemagglutination activity. Of the mushrooms evaluated, seven contained lectin activity. However, five (Agaricus bitorquis, Chlorophyllum brunneum, Coprinus comatus, Cortinarius sp. TWM 1710, and Omphalotus nidiformis) expressed lectin activity in only one of two collections tested. The two remaining lectin active mushroom species (Phlebopus marginatus and Psathyrella asperospora) possessed lectin activity with the same sugar specificity in both collections. Although lectins were identified with diverse specificity, lactose-specific lectin activity was most frequently identified, being present in Agaricus bitorquis, Copronus comatus, Omphalotus nidiformis, and Phlebopus marginatus. In contrast, Psathyrella asperospora, Cortinarius sp. TWM 1710, and Chlorophyllum brunneum were found to possess lectin activity specific for N-acetyl-D-glucosamine, galactose, and N-acetyl-neurammic acid, respectively. Significantly, the galactose-specific lectin activity identified in Cortinarius sp. TWM 1710 and the lactose-specific lectin activity in Phlebopus marginatus have not been previously reported.
authors.affiliation: Institute for Glycomics, Gold Coast campus, Griffith University, Queensland 4222, Australia.
authors.forename: Razina
authors.initials: R
authors.lastname: Rouf
authors.forename: Evelin
authors.initials: E
authors.lastname: Tiralongo
authors.forename: Anja
authors.initials: A
authors.lastname: Krahl
authors.forename: Karen
authors.initials: K
authors.lastname: Maes
authors.forename: Lina
authors.initials: L
authors.lastname: Spaan
authors.forename: Stefan
authors.initials: S
authors.lastname: Wolf
authors.forename: Tom W
authors.initials: TW
authors.lastname: May
authors.forename: Joe
authors.initials: J
authors.lastname: Tiralongo
mesh_terms: D000818:Animals; D001315:Australia; D001487:Basidiomycota / Q000737:chemistry*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D055808:Drug Discovery; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D011817:Rabbits
chemical_list: D037102:Lectins; D009439:Neuraminidase