title: Assay of in vitro osteoclast activity on dentine, and synthetic calcium phosphate bone substitutes.
abstract: Resorption of synthetic bone substitute materials is essential for the integration of these materials into the natural bone remodeling process. Osteoclast behavior in the presence of calcium phosphate bioceramics (CaPB) is partially understood, and a better understanding of the underlying mechanisms is expected to facilitate the development of new synthetic bone substitutes to improve bone regeneration. In the present study, our aim was to investigate osteoclastic resorption of various synthetic CaPB. We used neonatal total rabbit bone cells to generate osteoclasts. Osteoclast-generated resorption on dentine and multiple CaPB was investigated by quantifying the surface resorbed and measuring tartrate resistant acid phosphatase (TRAP) enzyme activity. In this study, we observed that osteoclastic cells responded in a different way to each substrate. Both dentine and CaPB were resorbed but the quantitative results for the surface resorbed and TRAP activity showed a specific response to each substrate and that increased mineral density seemed to inhibit osteoclast activity.
authors.affiliation: Osteo-Articular and Dental Tissue Engineering Laboratory LIOAD, Department of Periodontology, School of Dental Surgery, INSERM U791, Nantes, France.
authors.forename: Zahi
authors.initials: Z
authors.lastname: Badran
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Elise
authors.initials: E
authors.lastname: Verron
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bouler
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Gaël
authors.initials: G
authors.lastname: Grimandi
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Assem
authors.initials: A
authors.lastname: Soueidan
mesh_terms: D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D066298:In Vitro Techniques; D010010:Osteoclasts / Q000166:cytology*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate