title: Strong cation exchange chromatography in analysis of posttranslational modifications: innovations and perspectives.
abstract: Strong cation exchange (SCX) chromatography has been utilized as an excellent separation technique that can be combined with reversed-phase (RP) chromatography, which is frequently used in peptide mass spectrometry. Although SCX is valuable as the second component of such two-dimensional separation methods, its application goes far beyond efficient fractionation of complex peptide mixtures. Here I describe how SCX facilitates mapping of the protein posttranslational modifications (PTMs), specifically phosphorylation and N-terminal acetylation. The SCX chromatography has been mainly used for enrichment of these two PTMs, but it might also be beneficial for high-throughput analysis of other modifications that alter the net charge of a peptide.
authors.affiliation: Institute for Genomics, Biocomputing & Biotechnology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, MS 39762, USA. mje100@mafes.msstate.edu
authors.forename: Mariola J
authors.initials: MJ
authors.lastname: Edelmann
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D002412:Cations; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004331:Drosophila melanogaster / Q000378:metabolism; D006657:Histones / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D002412:Cations; D006657:Histones; D010455:Peptides; D011134:Polysaccharides; D013451:Sulfonic Acids; D001120:Arginine; D008239:Lysine