title: Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors.
abstract: Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.
authors.affiliation: Department of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ, UK.
authors.forename: Elena
authors.initials: E
authors.lastname: Cubero-Leon
authors.forename: A Mirel
authors.initials: AM
authors.lastname: Puinean
authors.forename: Pierre
authors.initials: P
authors.lastname: Labadie
authors.forename: Corina
authors.initials: C
authors.lastname: Ciocan
authors.forename: Naoki
authors.initials: N
authors.lastname: Itoh
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Kishida
authors.forename: Makoto
authors.initials: M
authors.lastname: Osada
authors.forename: Christophe
authors.initials: C
authors.lastname: Minier
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Hill
authors.forename: Jeanette M
authors.initials: JM
authors.lastname: Rotchell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D015536:Down-Regulation; D052244:Endocrine Disruptors / Q000633:toxicity*; D006066:Gonads / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D008969:Molecular Sequence Data; D049880:Mytilus edulis / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012722:Sewage; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D007527:Isoenzymes; D012333:RNA, Messenger; D012722:Sewage; D014874:Water Pollutants, Chemical; D051544:Cytochrome P-450 CYP3A