title: A multicentre study on the reliability of qualitative and quantitative nail-fold videocapillaroscopy assessment.
abstract: OBJECTIVE
  To investigate the inter- and intra-observer reliability of both qualitative and quantitative parameters used in the assessment of nail-fold capillaroscopy images.
  
  
  METHODS
  Fifty mosaic nail-fold images of healthy controls (n = 10), patients with primary RP (n = 10) and SSc (n = 30) were assessed in random order by two blinded observers on two occasions at centres in Sweden, UK and The Netherlands. Each image was therefore scored by six observers twice.
  
  
  RESULTS
  Inter- and intra-observer reliability of quantitative parameters showed substantial to almost perfect agreement [inter- and intra-observer weighted κ's for the number of widened capillaries was 0.75 and 0.87 and giant capillaries was 0.84 and 0.92, intra-class correlation coefficients (ICCs) for capillary density was 0.87 and 0.92 and total loop width was 0.94 and 0.98, respectively]. Qualitative parameters including architecture, avascularity, haemorrhage, crossed, ramified and bushy capillaries showed moderate to substantial inter-observer reproducibility (weighted κ ranging from 0.47 to 0.73), and substantial intra-observer repeatability (weighted κ ranging from 0.71 to 0.80), whereas the scoring of tortuous and bizarre capillaries showed poor inter-observer and substantial intra-observer agreement (inter-observer weighted κ's was 0.39 and 0.21 and intra-observer weighted κ's was 0.68 and 0.76, respectively).
  
  
  CONCLUSIONS
  All quantitative and certain qualitative parameters are highly reliable in terms of inter- and intra-observer agreement. A combination of parameters with the highest reliability should be incorporated into future capillaroscopic scoring systems in studies of prediction and monitoring of SSc spectrum disorders.
authors.affiliation: Department of Internal Medicine, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. hma.hofstee@vumc.nl
authors.forename: Herman M A
authors.initials: HM
authors.lastname: Hofstee
authors.forename: Erik H
authors.initials: EH
authors.lastname: Serné
authors.forename: Christopher
authors.initials: C
authors.lastname: Roberts
authors.forename: Roger
authors.initials: R
authors.lastname: Hesselstrand
authors.forename: Agneta
authors.initials: A
authors.lastname: Scheja
authors.forename: Tonia L
authors.initials: TL
authors.lastname: Moore
authors.forename: Marie
authors.initials: M
authors.lastname: Wildt
authors.forename: Joanne B
authors.initials: JB
authors.lastname: Manning
authors.forename: Anton
authors.initials: A
authors.lastname: Vonk Noordegraaf
authors.forename: Alexandre E
authors.initials: AE
authors.lastname: Voskuyl
authors.forename: Ariane L
authors.initials: AL
authors.lastname: Herrick
mesh_terms: D002196:Capillaries / Q000473:pathology; D006801:Humans; D020678:Microscopic Angioscopy / Q000379:methods*; D009262:Nails / Q000098:blood supply*; D015588:Observer Variation; D011928:Raynaud Disease / Q000473:pathology*; D015203:Reproducibility of Results; D012595:Scleroderma, Systemic / Q000473:pathology*; D016037:Single-Blind Method; D014741:Video Recording