title: Common polymorphisms in the P-selectin gene in women with recurrent spontaneous abortions.
abstract: BACKGROUND
  To investigate possible associations of P-selectin polymorphisms with idiopathic recurrent pregnancy loss (RPL).
  
  
  METHODS
  Study subjects comprised 270 consecutive RPL cases attending outpatient maternity services, and 322 multi-parous control women. P-selectin genotyping was done by PCR-RFLP and PCR-ASA methods.
  
  
  RESULTS
  The P-selectin variants rs1800807, rs1800805, and rs6127, were in Hardy Weinberg equilibrium, and low linkage disequilibrium was noted between the three studied SNPs. The frequency of rs6127 A allele (P<0.001I), but not rs1800807 C allele (P=0.957) or rs1800805 A allele (P=0.760), was higher in RPL cases than in control women. Significant differences in the distribution of rs6127 (P<0.001), but not rs1800807 (P=0.444) or rs1800805 (P=0.391) genotypes were seen between cases and controls, and only rs6127 showed a significant association with RPL, with increments of 2.65 and 4.96 in disease risk seen for heterozygous and homozygous carriers, respectively. Among the 8 three-locus Pselectin haplotypes constructed (rs1800807/rs1800805/rs6127), increased frequency of GGG (Pc=0.0249), CGG (Pc=0.0256), and CAG (Pc=0.0174) haplotypes, and lower frequency of CGA haplotype (Pc=0.0091) were seen in RPL cases, thus conferring disease susceptibility and protective nature to these haplotypes, respectively.
  
  
  CONCLUSIONS
  P-selectin gene polymorphisms and haplotypes contribute to RPL development.
authors.affiliation: Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
authors.forename: Maryam
authors.initials: M
authors.lastname: Dendana
authors.forename: Sondes
authors.initials: S
authors.lastname: Hizem
authors.forename: Kalthoum
authors.initials: K
authors.lastname: Magddoud
authors.forename: Safia
authors.initials: S
authors.lastname: Messaoudi
authors.forename: Walid
authors.initials: W
authors.lastname: Zammiti
authors.forename: Mona
authors.initials: M
authors.lastname: Nouira
authors.forename: Wassim Youssef
authors.initials: WY
authors.lastname: Almawi
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D019007:P-Selectin / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics
chemical_list: D019007:P-Selectin; C546937:SELP protein, human