title: Trans fatty acids enhanced β-amyloid induced oxidative stress in nerve growth factor differentiated PC12 cells.
abstract: The effects of trans fatty acids, elaidic acid (trans-9, C18:1) and linoelaidic acid (trans-9, trans-12 C18:2), at 20 or 40 μM in nerve growth factor differentiated PC12 cells with or without beta-amyloid peptide (Aβ) were examined. Elaidic acid treatment alone did not affect cell viability and oxidative injury associated markers (P > 0.05). However, co-treatments of elaidic acid and Aβ led to more reduction in mitochondrial membrane potential (MMP) and Na⁺-K⁺-ATPase activity, and more increase in DNA fragmentation and 8-hydroxydeoxyguanosine (8-OHdG) production than Aβ treatment alone (P < 0.05). Linoelaidic acid alone exhibited apoptotic and oxidative effects in cells via decreasing MMP and Na⁺-K⁺-ATPase activity, increasing reactive oxygen species (ROS) level, lowering glutathione content and glutathione peroxidase (GPX) activity (P < 0.05). The co-treatments of linoelaidic acid with Aβ further enhanced oxidative damage via enhancing the generation of ROS, nitrite oxide and 8-OHdG, elevating caspase-3, caspase-8 and nitric oxide synthase activities, as well as declining GPX, catalase and superoxide dismutase activities (P < 0.05). These results suggested that the interaction of linoelaidic acid and Aβ promoted oxidative stress and impaired mitochondrial functions in neuronal cells.
authors.affiliation: School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Shih-Jei
authors.initials: SJ
authors.lastname: Tsai
authors.forename: Wen-hu
authors.initials: WH
authors.lastname: Liu
authors.forename: Mei-chin
authors.initials: MC
authors.lastname: Yin
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D003603:Cytotoxins / Q000633:toxicity; D053938:DNA Fragmentation / Q000187:drug effects; D004357:Drug Synergism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D020932:Nerve Growth Factor / Q000494:pharmacology* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D044242:Trans Fatty Acids / Q000633:toxicity*
chemical_list: D016229:Amyloid beta-Peptides; D003603:Cytotoxins; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D044242:Trans Fatty Acids; C075222:amyloid beta-protein (1-42); D020932:Nerve Growth Factor