title: Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis.
abstract: OBJECTIVE
  Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. This study was aimed to the analysis of human arterial tissue secretome and to the quantitative comparison of healthy and atherosclerotic secretome to discover proteins with key roles in atherosclerosis development.
  
  
  RESULTS
  Secretomes from three biological replicates of human atherosclerotic coronary arteries (APC), preatherosclerotic coronaries (PC) and mammaries (M) were analyzed by LC-MS/MS. The identified proteins were submitted to Ingenuity Pathway Analysis (IPA) tool. Label-free MS/MS based quantification was performed and validated by immunohistochemistry. 64 proteins were identified in the 3 replicates of at least one of the 3 groups and 15 secreted proteins have not been previously reported in plasma. Four proteins were significantly released in higher amounts by mammary tissue: gelsolin, vinculin, lamin A/C and phosphoglucomutase 5.
  
  
  CONCLUSIONS
  The study of tissue secretome reveals key proteins involved in atherosclerosis which have not been previously reported in plasma. Novel proteins are here highlighted which could be potential therapeutic targets in clinical practice. This article is part of a Special Issue entitled: Proteomics: The clinical link.
authors.affiliation: Department of Immunology, IIS-Fundacion Jimenez Diaz, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cuesta
authors.forename: Maria G
authors.initials: MG
authors.lastname: Barderas
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Irene
authors.initials: I
authors.lastname: Zubiri
authors.forename: Aroa S
authors.initials: AS
authors.lastname: Maroto
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Darde
authors.forename: Tatiana
authors.initials: T
authors.lastname: Martin-Rojas
authors.forename: Felix
authors.initials: F
authors.lastname: Gil-Dones
authors.forename: Maria
authors.initials: M
authors.lastname: Posada-Ayala
authors.forename: Teresa
authors.initials: T
authors.lastname: Tejerina
authors.forename: Juan A
authors.initials: JA
authors.lastname: Lopez
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.forename: Gloria
authors.initials: G
authors.lastname: Alvarez-Llamas
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003331:Coronary Vessels / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D011506:Proteins; D020543:Proteome