title: Lifetimes of photosystem I and II proteins in the cyanobacterium Synechocystis sp. PCC 6803.
abstract: The half-life times of photosystem I and II proteins were determined using (15)N-labeling and mass spectrometry. The half-life times (30-75h for photosystem I components and <1-11h for the large photosystem II proteins) were similar when proteins were isolated from monomeric vs. oligomeric complexes on Blue-Native gels, suggesting that the two forms of both photosystems can interchange on a timescale of <1h or that only one form of each photosystem exists in thylakoids in vivo. The half-life times of proteins associated with either photosystem generally were unaffected by the absence of Small Cab-like proteins.
authors.affiliation: School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ 85287-4501, USA.
authors.forename: Danny C I
authors.initials: DC
authors.lastname: Yao
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Brune
authors.forename: Wim F J
authors.initials: WF
authors.lastname: Vermaas
mesh_terms: D002478:Cells, Cultured; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D006207:Half-Life; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D059748:Proteolysis; D046939:Synechocystis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D046912:Multiprotein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex