title: Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill.
abstract: A bacterial strain, designated NS31-3(T), was isolated from the wastewater of a paper mill. Cells of the isolate were obligately anaerobic, non-pigmented, non-motile, Gram-negative, short rods (0.7-1.0 × 1.4-2.5 µm). The isolate was able to grow on media containing 20% bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, D-xylose, L-arabinose, cellobiose, D-mannose, D-melezitose, D-raffinose, D-trehalose, D-mannitol, salicin and D-sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that the major fatty acids were anteiso-C(15:0), C(15:0) and iso-C(17:0) 3-OH and the predominant respiratory quinones were MK-9 and MK-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NS31-3(T) was related to members of genus Parabacteroides (91.2-93.2% sequence similarity); the isolate had the closest affinity with Parabacteroides merdae JCM 9497(T). The G+C content of the genomic DNA was 37.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain NS31-3(T) represents a novel species of the genus Parabacteroides, for which the name Parabacteroides chartae sp. nov. is proposed. The type strain is NS31-3(T) (=JCM 17797(T) =DSM 24967(T)).
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Tan
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Chu
authors.initials: C
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, PR China.
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D008969:Molecular Sequence Data; D010209:Paper; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D062065:Wastewater