title: Investigation of the relationship between oxidative stress and glucose signaling in Schizosaccharomyces pombe.
abstract: The invertase mutant defective in the glucose signaling pathway of Schizosaccharomyces pombe (ird11) is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in ird11, to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression (fbp1, fructose-1,6-bis-phosphatase; hxk2, hexokinase) and stress response (atf1 and pap1 transcription factors; ctt1, catalase; sod1, Cu,Zn superoxide dismutase) were analyzed using the quantitative real-time PCR technique. Oxidative stress response in ird11 seems to be affected by glucose signaling in a manner different from that caused by glucose deprivation.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, 34134 Istanbul, Turkey. bediag@istanbul.edu.tr
authors.forename: Bedia
authors.initials: B
authors.lastname: Palabiyik
authors.forename: Cenk
authors.initials: C
authors.lastname: Kig
authors.forename: Murat
authors.initials: M
authors.lastname: Pekmez
authors.forename: Levent
authors.initials: L
authors.lastname: Dalyan
authors.forename: Nazli
authors.initials: N
authors.lastname: Arda
authors.forename: Guler
authors.initials: G
authors.lastname: Temizkan
mesh_terms: D005947:Glucose / Q000378:metabolism*; D018384:Oxidative Stress; D000073718:Pancreatitis-Associated Proteins; D060888:Real-Time Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D013482:Superoxide Dismutase; D043324:beta-Fructofuranosidase; D005947:Glucose