title: [Correlation of stromal cell derived factor-1 with angiogenesis and cell apoptosis in myelodysplastic syndromes].
abstract: The study was aimed to investigate the expression of stromal cell derived factor (SDF-1) in bone marrow (BM) and its relation with apoptosis of BM CD34(+) cell and angiogenesis in myelodysplastic syndrome (MDS). 40 patients with MDS were divided into low-risk group and high-risk group according to IPSS score system. BM samples were collected. SDF-1 levels, the apoptosis of CD34(+) cells and microvessel density (MVD) of BM were detected by ELISA, flow cytometry and immunochemistry, respectively. The results showed that the SDF-1 level in MDS patients was significantly higher than that in normal controls(p < 0.05), and SDF-1 level in low-risk group was significantly higher than that in high-risk group. Apoptosis of CD34(+) cells significantly increased in low-risk group compared with other groups (p < 0.05). MVD in BM biopsy significantly increased in high-risk MDS group (p < 0.05), compared with low-risk MDS group which also had higher MVD than the control group (p < 0.05). Positive correlation was found between apoptosis of CD34(+) cells and SDF-1 levels in low-risk group, and SDF-1 level and MVD in high-risk group. It is concluded that the expression of SDF-1, apoptosis of BM CD34(+) cells and MVD were significantly abnormal in MDS patients, especially in different risk group, suggesting that SDF-1 level is related to cell apoptosis and angiogenesis.
authors.affiliation: Department of Hematology, Tianjin Medical University Cancer Hospital, Tianjin, China.
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Zhi
authors.forename: Yi-Zhuo
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhao
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Xiao-Xiong
authors.initials: XX
authors.lastname: Wu
authors.forename: Wan-Ming
authors.initials: WM
authors.lastname: Da
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D001853:Bone Marrow / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000378:metabolism* / Q000473:pathology*; D009389:Neovascularization, Pathologic; D055815:Young Adult
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12