title: Fabrication and evaluation of poly(epsilon-caprolactone)/silk fibroin blend nanofibrous scaffold.
abstract: In this study we investigated the blend electrospinning of poly(ϵ-caprolactone) (PCL) and silk fibroin (SF) to improve the biodegradability and biocompatibility of PCL-based nanofibrous scaffolds. Optimal conditions to fabricate PCL/SF (50/50) blend nanofiber were established for electrospinning using formic acid as a cosolvent and three-dimensional (3D) PCL/SF blend nanofibrous scaffolds were prepared by a modified electrospinning process using methanol coagulation bath. The physical properties of 2D PCL/SF blend nanofiber mats and 3D highly porous blend nanofibrous scaffolds were measured and compared. To evaluate cytocompatibility of the 3D blend scaffolds as compared to 3D PCL nanofibrous scaffold, normal human dermal fibroblasts were cultured. It is concluded that biodegradability and cytocompatibility could be improved for the 3D highly porous PCL/SF (50/50) blend nanofibrous scaffold prepared by blending PCL with SF in electrospinning. In addition to the blending of PCL and SF, the 3D structure and high porosity of electrospun nanofiber assemblies may also be important factors for enhancing the performance of scaffolds.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jun Sik
authors.initials: JS
authors.lastname: Lim
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Ki
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Kwang Gil
authors.initials: KG
authors.lastname: Lee
authors.forename: Seok Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Hae Yong
authors.initials: HY
authors.lastname: Kweon
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
mesh_terms: D005347:Fibroblasts / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; D047011:Silk; C016240:polycaprolactone; D005349:Fibroins