title: Study on the immune enhancement of different immunoadjuvants used in the pentavalent vaccine for turbots.
abstract: In this study, we investigated the immune enhancing effects of different adjuvants used in a pentavalent vaccine for turbots. The pentavalent vaccine consisted of inactive bacterial cells from five common pathogenic strains (Vibrio anguillarum, Vibrio scophtalmi, Edwardsiella tarda, Vibrio harveyi and Vibrio alginolyticus) and the adjuvants were astragalus polysaccharides (APS), propolis, and the Freund's complete adjuvant (FCA). Turbots were immunized with the pentavalent vaccine alone or with one of the adjuvants, and the immune efficiency was evaluated by measuring the activities of lysozyme (LSZ) and superoxide dismutase (SOD), and serum antibody titers. Fish were also challenged with the pathogens after immunization and the relative percent survival (RPS) was assessed. Our results showed that APS, propolis, and FCA had significant immune-enhancing effects on turbots as shown by the higher titers of antibodies against the pathogens, increased LSZ and SOD activities, and enhanced RPS after challenge with pathogens. Among the three adjuvants, FCA had the most significant immune synergistic effects with the vaccine, and APS and propolis had lower and similar immune synergies.
authors.affiliation: Colleges of Marine Life Sciences, Ocean University of China, Qingda 266003, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Wang
authors.initials: W
authors.lastname: Yingeng
authors.forename: Wang
authors.initials: W
authors.lastname: Qingyin
authors.forename: Deng
authors.initials: D
authors.lastname: Nannan
authors.forename: Liao
authors.initials: L
authors.lastname: Meijie
authors.forename: Qu
authors.initials: Q
authors.lastname: Jiangbo
authors.forename: Li
authors.initials: L
authors.lastname: Bin
authors.forename: Wang
authors.initials: W
authors.lastname: Lan
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001424:Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D001428:Bacterial Vaccines / Q000276:immunology*; D004755:Enterobacteriaceae / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control; D005412:Flatfishes / Q000276:immunology*; D007109:Immunity / Q000187:drug effects*; D007114:Immunization / Q000662:veterinary*; D009113:Muramidase / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D016019:Survival Analysis
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D013482:Superoxide Dismutase; D009113:Muramidase