title: [Effect of periostin on the function of human umbilical vein endothelial cells in acidic environment].
abstract: OBJECTIVE
  To observe the effect of recombinant periostin protein on the proliferation, apoptosis and migration of human umbilical vein endothelial cells (HUVECs) in acidic environment.
  
  
  METHODS
  HUVECs were divided into three groups: normal control (pH 7.31±0.03), acidic control (pH 6.95±0.05) and experimental groups [(periostin 100 μg/L, (pH 6.95±0.05)]. The cellular proliferation,apoptosis and migration were measured by Cell Counting Kit-8(CCK-8) assay, Annexin V-FITC with Flow Cytometer(at 60 h after periostin was given) and Transwell chamber assay (at 20 h after periostin was given).
  
  
  RESULTS
  In CCK-8 assay, the optical density values D450 nm (at 72 h after periostin was given) of normal control, acidic control and experimental groups were 0.71± 0.05, 0.62±0.04, 0.69±0.02, respectively. In the same order, the total apoptotic rate (%) of the three groups were 13.06±1.35, 16.95±0.46, 12.97±1.60, respectively; the numbers of migrated cells of the three groups were 67.45±11.88,44.89±8.67,64.60±9.63, respectively. Compared with normal control, the D(450 nm) value and the number of migrated cells of acidic control reduced 12.68% (P<0.01) and 33.44% (P<0.01) respectively. Otherwise, the total apoptotic rate (%) increased by 29.79% (P<0.05). Compared with acidic control, the D(450 nm) value and the number of migrated cells of experimental group increased by 11.29% (P<0.01) and 43.90% (P<0.01), respectively. Meantime, the total apoptotic rate (%) reduced 23.48% (P<0.05).
  
  
  CONCLUSIONS
  In acidic environment, the HUVECs' abilities of proliferation and migration were lower; while, the apoptotic value was raised. The recombinant periostin protein could counteract the acidic influence by enhancing the cellular abilities of proliferation and migration. Periostin could make endothelial cells reisist acidosis and keep growing to form blood vessels so that the local histiocytes could get enough nutrients for survival.
authors.affiliation: Department of Plastic Surgery, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Xin-lei
authors.initials: XL
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Bian
authors.forename: Ze-lian
authors.initials: ZL
authors.lastname: Qin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D011994:Recombinant Proteins