title: Common polymorphisms in CYP1A1, GSTM1, GSTT1, GSTP1 and XPD genes and endogenous DNA damage.
abstract: Endogenous DNA damage levels were analyzed in relation to polymorphisms in genes encoding phase I detoxifying enzyme-CYP1A1, phase II detoxifying enzymes-GSTM1, GSTT1, GSTP1 and enzyme involved in nucleotide excision repair-XPD. The study group consisted of 220 healthy non-smoking volunteers; 90 men and 130 woman, 25-60 years old (44 ± 10 years). The level of DNA damage (% DNA in tail) was evaluated by alkaline comet assay. The genetic variants were determined by restriction fragment length polymorphism PCR. The highest level of DNA damage (6.7%) was found in carriers of both: AA variant of XPD gene and M1 null variant of GSTM1 gene. The lowest level of DNA breaks (3.7%) was associated with the genotype GSTP1-AA/GSTM1 (+).
authors.affiliation: Department of Nutrigenomics, National Food and Nutrition Institute, Warsaw, Poland. mwlodarczyk@izz.waw.pl
authors.forename: Marta
authors.initials: M
authors.lastname: Wlodarczyk
authors.forename: Grazyna
authors.initials: G
authors.lastname: Nowicka
mesh_terms: D000328:Adult; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D019363:Cytochrome P-450 CYP1A1; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D051759:Xeroderma Pigmentosum Group D Protein; C569463:CYP1A1 protein, human; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human; C497292:ERCC2 protein, human