title: Correlation between liver  cancer occurrence and gene expression profiles in rat liver tissue.
abstract: Liver  cancer (LC) is generally characterized by malignant cell proliferation and  growth; it normally develops in stages that progress from non-specific injury  of the liver to liver fibrosis, liver cirrhosis, dysplasia nodules, and liver  carcinoma. We used a rat model of diethylnitrosamine (DENA)-induced LC; a Rat  Genome 230 2.0 Array was used to detect gene expression profile of liver  tissues from male rats 5, 8, 12, 16, and 18 weeks following the beginning of  DENA-induced LC. We found 909 known genes, including 637 up-regulated, 270  down-regulated, and two up/down-regulated genes, that were significantly  changed in expression. Among them, 108 genes were expressed at the 5th, 213 at  the 8th, 516 at the 12th, 698 at the 16th, and 506 at the 18th week of  DENA-induced LC. Methods in bioinformatics and systems biology were applied to  explore the correlation between the gene expression profile of rat liver tissue  and liver cancer occurrence at the transcriptional level; 23 physiological  activities were found to be associated with LC. Among these, eight  physiological activities, including stimulus response, inflammation and immune  response, oxidative reduction, cell proliferation, differentiation, migration,  adhesion, and angiogenesis were increased, implying that they could play  important roles in the occurrence and development of LC. In addition,  carbohydrate, lipid, and organic acid metabolism were decreased, suggesting  that liver injury induced by a carcinogenic agent has a negative effect on the  metabolism of fundamental substances.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang, Henan Province, China. xucs@x263.net
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
authors.forename: G P
authors.initials: GP
authors.lastname: Wang
authors.forename: L X
authors.initials: LX
authors.lastname: Zhang
authors.forename: C F
authors.initials: CF
authors.lastname: Chang
authors.forename: J
authors.initials: J
authors.lastname: Zhi
authors.forename: Y P
authors.initials: YP
authors.lastname: Hao
mesh_terms: D000818:Animals; D004052:Diethylnitrosamine; D004195:Disease Models, Animal; D018450:Disease Progression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D008099:Liver / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D004052:Diethylnitrosamine