title: High concentrations of circulating interleukin-6 and monocyte chemotactic protein-1 with low concentrations of interleukin-8 were associated with severe chikungunya fever during the 2009-2010 outbreak in Thailand.
abstract: The recent outbreak of Chikungunya virus in Thailand caused a rheumatic fever associated with considerable morbidity and fatalities. Thus, it is important to identify biomarker(s) of severe disease induced by this threatening arbovirus. Putative biomarkers in cases of chikungunya fever during an outbreak in the southern part of Thailand in 2009-2010 were identified. Sixty-two patients who had developed fever and myalgia, with or without arthralgia/arthritis, were enrolled and grouped into severe chikungunya fever (CHIKF) (n= 15), mild CHIKF (n= 20) and non-CHIKF (n= 27) to investigate circulating immunological mediators that might serve as markers of severity. Blood samples were taken at presentation (day 1) and 30 days later (day 30) and plasma concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-17, tumor necrosis factor-alpha, monocyte chemotactic protein-1 (MCP-1), matrix metalloproteinase-1, tissue inhibitor of matrix metalloproteinase-1 and viral load were measured by ELISA. On day 1, severe CHIKF and mild CHIKF groups had viral loads of 10(8.5)  and 10(8.3)  of RNA copies/mL, respectively. At presentation, all CHIKF patients had circulating concentrations of IL-6 and MCP-1 higher than did non-CHIKF patients, whereas amongst the CHKF patients, the severe CHIKF patients had higher IL-6 concentrations than did mild CHIKF patients. Interestingly, severe CHIKF patients had significantly lower concentrations of circulating IL-8 than the other groups of patients, suggesting that high concentrations of IL-6 and MCP-1 with low concentrations of IL-8 may be a determinant of severe chikungunya virus infection.
authors.affiliation: Department of Microbiology, Mahidol University, Rathchatewee, Bangkok.
authors.forename: Jindarat
authors.initials: J
authors.lastname: Lohachanakul
authors.forename: Weerawat
authors.initials: W
authors.lastname: Phuklia
authors.forename: Montri
authors.initials: M
authors.lastname: Thannagith
authors.forename: Tipparat
authors.initials: T
authors.lastname: Thonsakulprasert
authors.forename: Sukathida
authors.initials: S
authors.lastname: Ubol
mesh_terms: D000328:Adult; D018354:Alphavirus Infections / Q000097:blood* / Q000453:epidemiology / Q000821:virology; D018932:Chemokine CCL2 / Q000097:blood*; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004196:Disease Outbreaks; D005260:Female; D005334:Fever / Q000097:blood* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013785:Thailand; D055815:Young Adult
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D016209:Interleukin-8