title: [Proteomic analysis of the effect of noradrenalin on human pulmonary arterial smooth muscle cells].
abstract: OBJECTIVE
  To observe the effect of noradrenalin (NE) on human pulmonary arterial smooth muscle cells (PASMC) by using a proteomic approach.
  
  
  METHODS
  Human PASMC were cultured primarily in vitro. Experiments were performed in the 3(rd) to 5(th) passages of the cells. The human PASMC were cultured in serum-free medium for 24 h prior to treatment with either NE (10(-5) mol/L, the test group) or completed-serum culture medium (the control group) for 24 h. And then analysis via 2-DE gel electrophoresis and MALDI-TOF-MS was performed to display the different protein profiles of whole cell protein from cultures of the control and the NE-treatment group. Real-time RT-PCR and Western blot analysis were used to confirm the proteomic analysis.
  
  
  RESULTS
  The purity of the primary culture cells was about 99%. When the human PASMC were treated by NE, the expression of different groups of cellular proteins was changed, including cell cytoskeleton-associated proteins, cell signal-associated proteins, and glycolytic and metabolism-associated proteins. The results were confirmed using real-time RT-PCR and Western blot. NE enhanced the proliferation of human PASMC partly by affecting the expression of α-enolase.
  
  
  CONCLUSIONS
  The data suggest that a wide range of signaling pathways may be involved in NE-induced proliferation of human PASMC, and α-enolase associated pathway may be an important one.
authors.affiliation: Department of Respiratory Medicine, the Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou 213000, China. cougermx@yahoo.com.cn
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Yang
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Xie
authors.forename: Shi-jiang
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D040901:Proteomics; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D006867:Hydrolases; D009638:Norepinephrine