title: Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle).
abstract: This study was aimed to search new genetic variants in the bovine FABP4 gene as molecular markers for meat quality and carcass traits. PCR-RFLP analysis revealed that three SNPs located at nucleotide positions g.2834C>G, g.3533T>A, and g.3691G>A were identified based on a GenBank accession number (NC_007312.4). Sequence analysis revealed that SNPs were located in intron 1 (g.2834C>G) and 2 (g.3533T>A), and an exon 3 (g.3691G>A), showing allele frequencies as 0.592, 0.579, and 0.789, respectively. Genetic variabilities of heterozygosity (He) and polymorphic information contents (PIC) were estimated for g.2834C>G (0.608 and 0.531), g.3533T>A (0.615 and 0.539), and g.3691G>A (0.498 and 0.401) loci, respectively. A SNP located in the exon 3 of FABP4 was characterized and associated with desirable increases of MS (marbling scores) and MG (meat quality grades) in Hanwoo. The statistical analysis revealed that additive effects by GG genotypes in g.3691G>A SNP were significantly greater than AA genotypes in MS and MG traits. These findings suggest that the FABP4g.3691G>A SNP will be a useful candidate locus to maximize economic benefits for cattle populations.
authors.affiliation: Division of Animal Science and Resources, College of Life Science and Natural Resources, Sangji University, 660 Usnadong, Wonju, Gangwondo, 220-702, South Korea.
authors.forename: Sung-Chul
authors.initials: SC
authors.lastname: Shin
authors.forename: Jae-Pil
authors.initials: JP
authors.lastname: Heo
authors.forename: Eui-Ryong
authors.initials: ER
authors.lastname: Chung
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D005838:Genotype; D006579:Heterozygote; D016018:Least-Squares Analysis; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D056910:Republic of Korea
chemical_list: D050556:Fatty Acid-Binding Proteins