title: Purification of L-alpha glycerylphosphorylcholine by column chromatography.
abstract: Colorless L-alpha glycerylphosphorylcholine (L-α-GPC) was obtained at 99.8% purity, 69.8% recovery, and with a specific rotation of -2.5° via a five-step procedure. L-α-GPC was first produced by phospholipase A(1) hydrolysis of soy lecithin powder. Ca(2+) and Cl(-) were then effectively removed using two successive 001×7 cation and D311 anion exchange resin column chromatography procedures. Silica gel column chromatography and decoloration with active carbon were then applied to remove remaining impurities and colorant. Characterization of the L-α-GPC product was well in agreement with the standard. The resin and silica gel showed remarkable ability for L-α-GPC isolation after 10 uses. Thus, this study presents a simple and cost-effective method for preparing L-α-GPC with high yield and purity, low cost, and environmental friendliness, and encourages future investigation into its adaptation for industrial applications.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu Province, China.
authors.forename: Kangyi
authors.initials: K
authors.lastname: Zhang
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang
authors.forename: Yuanfa
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D002118:Calcium / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D017743:Equipment Reuse; D005997:Glycerylphosphorylcholine / Q000302:isolation & purification*; D007475:Ion Exchange Resins; D054709:Lecithins / Q000737:chemistry / Q000378:metabolism; D000432:Methanol; D054466:Phospholipases A1 / Q000378:metabolism; D058428:Silica Gel; D013025:Glycine max
chemical_list: D002712:Chlorides; D007475:Ion Exchange Resins; D054709:Lecithins; D058428:Silica Gel; D005997:Glycerylphosphorylcholine; D054466:Phospholipases A1; D002118:Calcium; D000432:Methanol