title: Fabrication of hierarchical hybrid structures using bio-enabled layer-by-layer self-assembly.
abstract: Development of versatile and flexible assembly systems for fabrication of functional hybrid nanomaterials with well-defined hierarchical and spatial organization is of a significant importance in practical nanobiotechnology applications. Here we demonstrate a bio-enabled self-assembly technique for fabrication of multi-layered protein and nanometallic assemblies utilizing a modular gold-binding (AuBP1) fusion tag. To accomplish the bottom-up assembly we first genetically fused the AuBP1 peptide sequence to the C'-terminus of maltose-binding protein (MBP) using two different linkers to produce MBP-AuBP1 hetero-functional constructs. Using various spectroscopic techniques, surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR), we verified the exceptional binding and self-assembly characteristics of AuBP1 peptide. The AuBP1 peptide tag can direct the organization of recombinant MBP protein on various gold surfaces through an efficient control of the organic-inorganic interface at the molecular level. Furthermore using a combination of soft-lithography, self-assembly techniques and advanced AuBP1 peptide tag technology, we produced spatially and hierarchically controlled protein multi-layered assemblies on gold nanoparticle arrays with high molecular packing density and pattering efficiency in simple, reproducible steps. This model system offers layer-by-layer assembly capability based on specific AuBP1 peptide tag and constitutes novel biological routes for biofabrication of various protein arrays, plasmon-active nanometallic assemblies and devices with controlled organization, packing density and architecture.
authors.affiliation: Department of Material Science and Engineering, Genetically Engineered Materials Science and Engineering Center (GEMSEC), University of Washington, Seattle, Washington 98195, USA.
authors.forename: Marketa
authors.initials: M
authors.lastname: Hnilova
authors.forename: Banu Taktak
authors.initials: BT
authors.lastname: Karaca
authors.forename: James
authors.initials: J
authors.lastname: Park
authors.forename: Carol
authors.initials: C
authors.lastname: Jia
authors.forename: Brandon R
authors.initials: BR
authors.lastname: Wilson
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sarikaya
authors.forename: Candan
authors.initials: C
authors.lastname: Tamerler
mesh_terms: D001709:Biotechnology / Q000379:methods*; D006046:Gold / Q000378:metabolism*; D053768:Metal Nanoparticles; D055503:Protein Multimerization; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins; D006046:Gold