title: Crucial aspects of high performance thin layer chromatography quantitative validation. The case of determination of rosmarinic acid in different matrices.
abstract: A new HPTLC method was envisaged to determine rosmarinic acid (RA) in different matrices with the aim of testing the influence of optimizing the main HPTLC operative parameters in view of a more stringent validation process. HPTLC LiChrospher silica gel 60 F254s, 20 cm × 10 cm, plates with toluene:ethyl formate:formic acid (6:4:1, v/v) as the mobile phase were used. Densitometric determinations were performed in reflectance mode at 330 nm. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. RA was quantified in the range of 132-660 ng with RSD of repeatability and intermediate precision not exceeding 2.0% and accuracy within the acceptance limits. The method was tested on several commercial preparations containing RA in different amounts.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Firenze, Italy. coran@unifi.it
authors.forename: Silvia A
authors.initials: SA
authors.lastname: Coran
authors.forename: Stefano
authors.initials: S
authors.lastname: Mulas
authors.forename: Nadia
authors.initials: N
authors.lastname: Mulinacci
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards*; D002855:Chromatography, Thin Layer / Q000379:methods* / Q000592:standards*; D002934:Cinnamates / Q000032:analysis*; D053630:Depsides / Q000032:analysis*; D015203:Reproducibility of Results; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid