title: Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor.
abstract: Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 16681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection.
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kinoshita
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Maria Del Carmen
authors.initials: MDC
authors.lastname: Parquet
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Muhareva
authors.initials: M
authors.lastname: Raekiansyah
authors.affiliation: Division of Immunology, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kimura
authors.affiliation: Division of Immunology, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yui
authors.affiliation: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
authors.forename: Mohammed Alimul
authors.initials: MA
authors.lastname: Islam
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Hasebe
authors.affiliation: Department of Virology, Institute of Tropical Medicine, Nagasaki University and Global COE Program, Nagasaki, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000378:metabolism / Q000502:physiology*; D004198:Disease Susceptibility / Q000821:virology; D015870:Gene Expression; D020868:Gene Silencing; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D020014:K562 Cells / Q000821:virology; D011991:Receptors, Virus / Q000378:metabolism*; D019595:Severe Dengue / Q000378:metabolism / Q000821:virology*; D053669:Syndecan-2 / Q000378:metabolism*; D014709:Vero Cells; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D006497:Heparan Sulfate; D011991:Receptors, Virus; D053669:Syndecan-2