title: Acceptable warm ischemia time of tracheal grafts from non-heart-beating donors in rats.
abstract: OBJECTIVE
  Warm ischemia (WI)-induced airway complications are common in clinical lung transplantation. However, the acceptable WI time of tracheal grafts from non-heart-beating donors (NHBDs) is unknown. The purpose of this study was to determine the acceptable WI time by observing tracheal epithelial regeneration among NHBD.
  
  
  METHODS
  Forty-eight rats were randomly divided into four groups (each with 12 rats): WI-0 minutes (group A), WI-30 minutes (group B), WI-45 minutes (group C), and WI-60 minutes (group D). In each group, the tracheas from 6 rats were imbedded in the greater omentum of 6 other rats. Fourteen days later, the transplanted trachea was obtained from the recipient to evaluate epithelial thickness and regeneration. Six tracheas were obtained from living donors as a control group.
  
  
  RESULTS
  There were no significant differences in tracheal transplantation time (mean, 17.66±1.21 minutes). There were no significant differences in epithelial thickness and regeneration between the controls and groups A, B, and C (P<.05). Group D showed no normal epithelial structure of the trachea only with monolayer cells.
  
  
  CONCLUSIONS
  The time limits of tolerance to WI of tracheal grafts from NHBDs may be 45 minutes.
authors.affiliation: Department of Thoracic Surgery, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: J-Q
authors.initials: JQ
authors.lastname: Han
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Cui
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: G-B
authors.initials: GB
authors.lastname: Zhao
authors.forename: Y-Z
authors.initials: YZ
authors.lastname: Xin
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D008297:Male; D009852:Omentum / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D020545:Respiratory Mucosa / Q000473:pathology / Q000637:transplantation*; D013997:Time Factors; D014132:Trachea / Q000473:pathology / Q000637:transplantation*; D052096:Warm Ischemia; D014945:Wound Healing