title: Increasing antibiotic activity against a multidrug-resistant Acinetobacter spp by essential oils of Citrus limon and Cinnamomum zeylanicum.
abstract: The genus Acinetobacter has gained importance in recent years due to involvement in serious infections and antimicrobial resistance. Many plants have been evaluated not only for direct antimicrobial activity, but also as resistance modifying agents. The Essential oil of Citrus limon (EOCL) addition at 156.25 µgmL(-1) (MIC/8) sub-inhibitory concentration in the growth medium led to MIC decrease for amikacin, imipenem and meropenem. The Essential oil of Cinnamomum zeylanicum (EOCZ) addition at 78.125 µg mL(-1) (MIC/8) sub-inhibitory concentrations in the growth medium caused drastic MIC reduction of amikacin. Results of combining antibiotics and essential oils had shown us a synergistic effect with both essential oils/amikacin combinations. An additive effect was observed with the combinations of both essential oils and gentamicin. The results of this study suggest that essential oil of C. limon and C. zeylanicum may suppress the growth of Acinetobacter species and could be a source of metabolites with antibacterial modifying activity.
authors.affiliation: Laboratory of Mycology, Department of Pharmaceutical Sciences, Federal University of Paraíba, 58051-970 João Pessoa, Paraíba, Brazil. felipeqsguerra@gmail.com
authors.forename: Felipe Queiroga Sarmento
authors.initials: FQ
authors.lastname: Guerra
authors.forename: Juliana Moura
authors.initials: JM
authors.lastname: Mendes
authors.forename: Janiere Pereira de
authors.initials: JP
authors.lastname: Sousa
authors.forename: Maria F B
authors.initials: MF
authors.lastname: Morais-Braga
authors.forename: Bernadete Helena Cavalcante
authors.initials: BH
authors.lastname: Santos
authors.forename: Henrique Douglas
authors.initials: HD
authors.lastname: Melo Coutinho
authors.forename: Edeltrudes de Oliveira
authors.initials: Ede O
authors.lastname: Lima
mesh_terms: D000150:Acinetobacter / Q000187:drug effects* / Q000254:growth & development; D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002935:Cinnamomum zeylanicum / Q000737:chemistry*; D002957:Citrus / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D004357:Drug Synergism; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D013845:Thienamycins / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D013845:Thienamycins; D000583:Amikacin; D000077731:Meropenem