title: Study of an aquifer contaminated by ethyl tert-butyl ether (ETBE): site characterization and on-site bioremediation.
abstract: Ethyl tert-butyl ether (ETBE) was detected at high concentration (300mgL(-1)) in the groundwater below a gas-station. No significant carbon neither hydrogen isotopic fractionation of ETBE was detected along the plume. ETBE and BTEX biodegradation capacities of the indigenous microflora Pz1-ETBE and of a culture (MC-IFP) composed of Rhodococcus wratislaviensis IFP 2016, Rhodococcus aetherivorans IFP 2017 and Aquincola tertiaricarbonis IFP 2003 showed that ETBE and BTEX degradation rates were in the same range (ETBE: 0.91 and 0.83 mg L(-1)h(-1) and BTEX: 0.64 and 0.82 mg L(-1)h(-1), respectively) but tert-butanol (TBA) accumulated transiently at a high level using Pz1-ETBE (74 mg L(-1)). An on-site pilot plant (2m(3)) filled with polluted groundwater and inoculated by MC-IFP, successfully degraded four successive additions of ETBE and gasoline. However, an insignificant ETBE isotopic fractionation was also accompanying this decrease which suggested the involvement of low fractionating-strains using EthB enzymes, but required of additional proofs. The ethB gene encoding a cytochrome P450 involved in ETBE biodegradation (present in R. aetherivorans IFP 2017) was monitored by quantitative real-time polymerase chain reaction (q-PCR) on DNA extracted from water sampled in the pilot plant which yield up to 5×10(6) copies of ethB gene per L(-1).
authors.affiliation: IFP Energies nouvelles, 1 et 4 avenue de Bois-Préau, 92852 Rueil-Malmaison, France. francoise.fayolle@ifpen.fr
authors.forename: Françoise
authors.initials: F
authors.lastname: Fayolle-Guichard
authors.forename: Jonathan
authors.initials: J
authors.lastname: Durand
authors.forename: Mathilde
authors.initials: M
authors.lastname: Cheucle
authors.forename: Mònica
authors.initials: M
authors.lastname: Rosell
authors.forename: Rory Julien
authors.initials: RJ
authors.lastname: Michelland
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Tracol
authors.forename: Françoise
authors.initials: F
authors.lastname: Le Roux
authors.forename: Geneviève
authors.initials: G
authors.lastname: Grundman
authors.forename: Olivier
authors.initials: O
authors.lastname: Atteia
authors.forename: Hans H
authors.initials: HH
authors.lastname: Richnow
authors.forename: Alain
authors.initials: A
authors.lastname: Dumestre
authors.forename: Yves
authors.initials: Y
authors.lastname: Benoit
mesh_terms: D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000201:enzymology / Q000254:growth & development; D005019:Ethyl Ethers / Q000302:isolation & purification*; D005602:France; D005742:Gasoline; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology; D012240:Rhodococcus / Q000201:enzymology / Q000254:growth & development; D014872:Water Movements; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D018508:Water Purification / Q000379:methods*
chemical_list: D005019:Ethyl Ethers; D005742:Gasoline; D014874:Water Pollutants, Chemical; C098546:ethyl tert-butyl ether