title: [Effect of ischemic postconditioning on oxidative stress and structural tissue changes in intestinal warm ischemic and autotransplantation models].
abstract: OBJECTIVE
  Our study investigated the effect of ischemic postconditioning (IPO) in intestinal warm ischemia/reperfusion (I/R) and autotransplantation models.
  
  
  METHODS
  Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours in white domestic pigs (n = 15). Prior to 3 hours reperfusion the intestine was postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion (IPO protocol). In the cold ischemia group (n = 15) the bowel was preserved in University of Wisconsin solution for 1, 3, and 6 hours. Prior to 3 hours reperfusion IPO protocol was applied, too. Tissue samples were collected after laparotomy (control) and at the end of the reperfusion periods. As far as oxidative stress markers, malondialdehyde and reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were determined. Tissue damage was evaluated by qualitative (Park-classification) and quantitative (Scion Image) methods.
  
  
  RESULTS
  As regards oxidative stress parameters, lipidperoxidation decreased and the protective effect of endogenous antioxidants (GSH, SOD) retained significantly by IPO procedure at the end of reperfusion. Tissue injury correlated significantly by the duration of warm ischemia and cold preservation. Quantitative analysis demonstrated that IPO ameliorated tissue injury in each group (p < 0.05).
  
  
  CONCLUSIONS
  IPO significantly attenuated intestinal oxidative stress and morphological damages in warm and cold I/R models.
authors.affiliation: Zala Megyei Kórház, Általános Sebészeti Osztály, Zalaegerszeg. nedvigklari@gmail.com
authors.forename: Klára
authors.initials: K
authors.lastname: Nedvig
authors.forename: Edina
authors.initials: E
authors.lastname: Völgyi
authors.forename: György
authors.initials: G
authors.lastname: Wéber
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Röth
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferencz
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D007328:Insulin; D007422:Intestines / Q000098:blood supply / Q000473:pathology*; D007511:Ischemia / Q000378:metabolism*; D057775:Ischemic Postconditioning; D007813:Laparotomy; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D019188:Organ Preservation Solutions; D018384:Oxidative Stress; D011887:Raffinose; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D034421:Sus scrofa; D013997:Time Factors; D014182:Transplantation, Autologous; D052096:Warm Ischemia
chemical_list: D000975:Antioxidants; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008315:Malondialdehyde; D000493:Allopurinol; D013482:Superoxide Dismutase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose