title: Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis.
abstract: OBJECTIVE
  To assess the diagnostic efficacy of the currently most widely used indirect hemagglutination assay (IHA) and enzyme-linked immunosorbent assay (ELISA) for detection of Schistosoma japonicum human infections.
  
  
  METHODS
  A comprehensive search was undertaken from China National Knowledge Infrastructure, Wanfang Database, VIP Database, PubMed, Cochrane Library, Science Citation Index Expanded, Proquest, and the inclusion and exclusion criteria were strictly settled. The funnel plot was used to assess the publication bias, Cochran's Q test was employed to measure the homogeneity between studies, a summary receiver operating characteristic (SROC) curve was used to compare the diagnostic accuracy between the IHA and ELISA qualitatively by means of the Weighted Least Square method, the Ordinary Least Square method and the Robust regression method, and the diagnostic odds ratio (DOR) was drawn to compare the accuracy quantitatively.
  
  
  RESULTS
  Out of 785 publications, 19 papers were eventually selected for analysis. Literature quality assessment indicated that minor publication bias existed in studies pertaining IHA test, but no bias was found in literatures regarding ELISA test. The heterogeneity test showed a heterogeneity between studies was present (χ(2)=466.07 and 34.67, both P values<0.0001). The areas under the SROC curves of IHA were all higher than that of ELISA test using the three methods (Weighted Least Square method: 0.766 vs. 0.695, Ordinary Least Square method: 0.826 vs. 0.741, Robust regression: 0.815 vs. 0.715). The TPR* values for IHA and ELISA were 0.710, 0.759, 0.749, and 0.650, 0.686 and 0.666, respectively, and OR values were 5.997, 9.937, 8.893, and 3.432, 4.784 and 3.959, respectively. The DOR of IHA was 9.41 (95% CI: 4.88-18.18), and 4.78 (95% CI: 3.21-7.13) for ELISA.
  
  
  CONCLUSIONS
  All above results revealed that the diagnostic performance of IHA is better than that of ELISA. However, taking into account their unsatisfactory diagnostic value in areas with low infection intensity, a search for a better diagnostic test that can be applied in field situations in China should be given high priority.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, 117 Yangxiang, Meiyuan, Wuxi, Jiangsu Province, People's Republic of China. wangweijipd@yahoo.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Youzi
authors.initials: Y
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.forename: Yuntian
authors.initials: Y
authors.lastname: Xing
authors.forename: Guoli
authors.initials: G
authors.lastname: Qu
authors.forename: Jianrong
authors.initials: J
authors.lastname: Dai
authors.forename: Yousheng
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D016018:Least-Squares Analysis; D016017:Odds Ratio; D012372:ROC Curve; D012549:Schistosoma japonicum / Q000276:immunology / Q000302:isolation & purification*; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D012680:Sensitivity and Specificity