title: Arabidopsis mitochondrial protein TIM50 affects hypocotyl cell elongation through intracellular ATP level.
abstract: The plant hypocotyl is an excellent model for the analysis of cell elongation. We have characterized a knockout mutant of the Arabidopsis TIM50 gene that showed a reduction in the hypocotyls length of etiolated seedlings. We also found that a knockout of TIM50 caused enlargement and deformation of the mitochondrial structure and a reduction in intracellular ATP levels. TIM50 is a component of the mitochondrial TIM23 inner membrane protein complex and is involved in the import of mitochondrial proteins. The short hypocotyl phenotype was recovered by the addition of Compound C, an inhibitor of AMPK. Thus, the mitochondrial ATP level controls cell elongation in Arabidopsis hypocotyls through possible signaling via AMPK.
authors.affiliation: Plant Synthetic Genomics Research Division, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244-0813, Japan.
authors.forename: Shailesh
authors.initials: S
authors.lastname: Kumar
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hongo
authors.forename: Arata
authors.initials: A
authors.lastname: Yoneda
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hara
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Hamasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D005434:Flow Cytometry; D055786:Gene Knockout Techniques; D018546:Hypocotyl / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D016254:Mutagenesis, Insertional; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; C036483:T-DNA; C575165:TIM50 protein, Arabidopsis; D000255:Adenosine Triphosphate; D055372:AMP-Activated Protein Kinases