title: Molecular systematics of Polygonum minus Huds. based on ITS sequences.
abstract: Plastid trnL-trnF and nuclear ribosomal ITS sequences were obtained from selected wild-type individuals of Polygonum minus Huds. in Peninsular Malaysia. The 380 bp trnL-trnF sequences of the Polygonum minus accessions were identical. Therefore, the trnL-trnF failed to distinguish between the Polygonum minus accessions. However, the divergence of ITS sequences (650 bp) among the Polygonum minus accessions was 1%, indicating that these accessions could be distinguished by the ITS sequences. A phylogenetic relationship based on the ITS sequences was inferred using neighbor-joining, maximum parsimony and Bayesian inference. All of the tree topologies indicated that Polygonum minus from Peninsular Malaysia is unique and different from the synonymous Persicaria minor (Huds.) Opiz and Polygonum kawagoeanum Makino.
authors.affiliation: Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia; E-Mails:  hamidunb@yahoo.com  (H.B.);  nataqain@ukm.my  (S.N.B.).
authors.forename: Hamidun
authors.initials: H
authors.lastname: Bunawan
authors.forename: Chee Yen
authors.initials: CY
authors.lastname: Choong
authors.forename: Badrul Munir
authors.initials: BM
authors.lastname: Md-Zain
authors.forename: Syarul Nataqain
authors.initials: SN
authors.lastname: Baharum
authors.forename: Normah Mohd
authors.initials: NM
authors.lastname: Noor
mesh_terms: D001499:Bayes Theorem; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D008296:Malaysia; D010802:Phylogeny; D018087:Plastids / Q000235:genetics; D028502:Polygonum / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer