title: Detection of yeasts in blood cultures by the Luminex xTAG fungal assay.
abstract: A multiplex-PCR Luminex xMAP bead probe fluid array using xTAG analyte-specific reagents (multiplex xTAG fungal ASR assay) was employed for detection of clinically significant Candida species, Cryptococcus neoformans, Histoplasma capsulatum, and Blastomyces dermatitidis from blood cultures. We tested 132 blood cultures negative (n = 10) or positive (n = 97) for yeasts and/or bacteria (n = 25). The assay showed sensitivity and specificity of 100% and 99%, respectively. The xTAG fungal ASR assay is a rapid assay that allows simultaneous identification of multiple yeast species.
authors.affiliation: Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA.
authors.forename: Joan-Miquel
authors.initials: JM
authors.lastname: Balada-Llasat
authors.forename: Heidi
authors.initials: H
authors.lastname: LaRue
authors.forename: Kamal
authors.initials: K
authors.lastname: Kamboj
authors.forename: Lisa
authors.initials: L
authors.lastname: Rigali
authors.forename: Debra
authors.initials: D
authors.lastname: Smith
authors.forename: Keelie
authors.initials: K
authors.lastname: Thomas
authors.forename: Preeti
authors.initials: P
authors.lastname: Pancholi
mesh_terms: D001769:Blood / Q000382:microbiology*; D016469:Fungemia / Q000175:diagnosis*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000302:isolation & purification*