title: Complete microscale profiling of tumor microangiogenesis: a microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization.
abstract: Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (~1 μm) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (~1 ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3-10 μm) microvessels were found to constitute >80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties.
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei 115, Taiwan.
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Chien
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Kempson
authors.forename: Cheng Liang
authors.initials: CL
authors.lastname: Wang
authors.forename: H H
authors.initials: HH
authors.lastname: Chen
authors.forename: Yeukuang
authors.initials: Y
authors.lastname: Hwu
authors.forename: N Y
authors.initials: NY
authors.lastname: Chen
authors.forename: T K
authors.initials: TK
authors.lastname: Lee
authors.forename: Kelvin K-C
authors.initials: KK
authors.lastname: Tsai
authors.forename: Ming-Sheng
authors.initials: MS
authors.lastname: Liu
authors.forename: Kwang-Yu
authors.initials: KY
authors.lastname: Chang
authors.forename: C S
authors.initials: CS
authors.lastname: Yang
authors.forename: G
authors.initials: G
authors.lastname: Margaritondo
mesh_terms: D000465:Algorithms; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology; D001466:Barium Sulfate; D000068258:Bevacizumab; D003287:Contrast Media; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008852:Microradiography / Q000295:instrumentation / Q000379:methods*; D055806:Microvessels / Q000187:drug effects / Q000473:pathology*; D053758:Nanoparticles; D009389:Neovascularization, Pathologic / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology; D017356:Synchrotrons; D059016:Tumor Microenvironment; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D061067:Antibodies, Monoclonal, Humanized; D003287:Contrast Media; D001466:Barium Sulfate; D000068258:Bevacizumab