title: Mutagenicity and clastogenicity evaluation of tacrine by Ames and micronucleus assays.
abstract: Tacrine was evaluated for its mutagenic and clastogenic activities using the Ames bacterial reverse-mutation assay and the rodent bone marrow micronucleus assay. Tacrine was tested for mutagenic potential at six different concentrations, with 1,250 µg/plate as the highest concentration, followed by five lower concentrations with 2-fold spacing. In clastogenic evaluation, tacrine was administered orally to Wistar rats for 2 days at 5, 10, and 20 mg/kg body weights to assess micronucleus induction in bone marrow erythrocytes. In the Ames assay, tacrine showed nonmutagenicity in four tester strains of Salmonella typhimurium viz. TA98, TA100, TA102, and TA1535, but it was found to be mutagenic in the TA1537 tester strain, both in the presence and absence of a metabolic activation system. Tacrine was found to be nonclastogenic on bone marrow cells of rats at all doses tested and was found to be mutagenic in only the TA1537 strain of Salmonella.
authors.affiliation: Suven Life Science Ltd., Hyderabad, India. msadik_mulla@suven.com
authors.forename: Mohmad Sadik
authors.initials: MS
authors.lastname: Mulla
authors.forename: Ravichandra Bhadravathi
authors.initials: RB
authors.lastname: Vedamurthy
authors.forename: Santanu
authors.initials: S
authors.lastname: Jana
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Pandey
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Goyal
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nirogi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D018697:Nootropic Agents / Q000008:administration & dosage / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D013619:Tacrine / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D009153:Mutagens; D018697:Nootropic Agents; D013619:Tacrine