title: Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe.
abstract: Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.
authors.affiliation: School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Zheng-Yun
authors.initials: ZY
authors.lastname: Zhao
authors.forename: Marie
authors.initials: M
authors.lastname: San
authors.forename: Jean-Louis H A
authors.initials: JL
authors.lastname: Duprey
authors.forename: John R
authors.initials: JR
authors.lastname: Arrand
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Vyle
authors.forename: James H R
authors.initials: JH
authors.lastname: Tucker
mesh_terms: D000873:Anthracenes / Q000737:chemistry; D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008297:Male; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000873:Anthracenes; D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA