title: Proteome and phosphoproteome of primary cultured pig urothelial cells.
abstract: Epithelial tissue lining the inner side of the urinary bladder is the most common target for bladder cancer-related diseases. Bladders of freshly slaughtered pigs were utilised for a comprehensive analysis of the proteome and phosphoproteome of bladder epithelial cells. Following protein separation by 2-D gel electrophoresis and identification by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) the first proteome and phosphoproteome maps of pig urinary bladder epithelial cells (PUBEC) were established. A total of 120 selected protein spots were identified. By using the La(3+)  enrichment method further developed in our laboratory we identified 31 phosphoproteins with minimal contamination by non-phosphopeptides. The 2-DE map of pig urothelial cells may prove as a useful tool for studies on uroepithelial biology, and the analysed phosphoproteins expression pattern, together with the whole cell proteome, will be helpful for identifying the proteins involved in bladder-related diseases.
authors.affiliation: Institute of Hygiene and Occupational Medicine, University Hospital Essen, Essen, Germany.
authors.forename: Nisha
authors.initials: N
authors.lastname: Verma
authors.forename: Carolin
authors.initials: C
authors.lastname: Bäuerlein
authors.forename: Mario
authors.initials: M
authors.lastname: Pink
authors.forename: Albert W
authors.initials: AW
authors.lastname: Rettenmeier
authors.forename: Simone
authors.initials: S
authors.lastname: Schmitz-Spanke
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D001743:Urinary Bladder / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D019459:Urothelium / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D020543:Proteome