title: Occurrence of putative virulence genes in arcobacter species isolated from humans and animals.
abstract: Interest in arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. Since then, studies worldwide have reported the occurrence of arcobacters on food and in food production animals and have highlighted possible transmission, especially of Arcobacter butzleri, to the human population. In humans, arcobacters are associated with enteritis and septicemia. To assess their clinical relevance for humans and animals, evaluation of potential virulence factors is required. However, up to now, little has been known about the mechanisms of pathogenicity. Because of their close phylogenetic affiliation to the food-borne pathogen Campylobacter and their similar clinical manifestations, the presence of nine putative Campylobacter virulence genes (cadF, ciaB, cj1349, hecA, hecB, irgA, mviN, pldA, and tlyA) previously identified in the recent Arcobacter butzleri ATCC 49616 genome sequence was determined in a large set of human and animal Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii strains after the development of rapid and accurate PCR assays and confirmed by sequencing and dot blot hybridization.
authors.affiliation: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
authors.forename: Laid
authors.initials: L
authors.lastname: Douidah
authors.forename: Lieven
authors.initials: L
authors.lastname: de Zutter
authors.forename: Julie
authors.initials: J
authors.lastname: Baré
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Olivier
authors.initials: O
authors.lastname: Vandenberg
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Van den Abeele
authors.forename: Kurt
authors.initials: K
authors.lastname: Houf
mesh_terms: D000818:Animals; D020640:Arcobacter / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D002167:Campylobacter / Q000235:genetics; D005798:Genes, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors