title: Structural studies of the O-antigenic polysaccharide from Plesiomonas shigelloides strain AM36565.
abstract: The structure of the repeating unit of the O-antigenic polysaccharide from Plesiomonas shigelloides strain AM36565 has been determined. Component analysis and (1)H and (13)C NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by (1)H,(13)C heteronuclear multiple-bond correlation, (1)H,(1)H-NOESY, and (1)H,(13)C-HSQC-(1)H,(1)H-NOESY experiments. The O-antigen polysaccharide is composed of repeating units with the following structure: →3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→4)[β-D-GalpNAc-(1→3)]-α-D-GlcpNAc-(1→, in which the monosaccharide side-chain substitutes the backbone in half of the repeating units. A matrix-assisted laser desorption/ionization mass spectrometry experiment suggested that the polysaccharide consists of two regions, one with tetrasaccharide repeating units and one with trisaccharide repeating units.
authors.affiliation: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Elin
authors.initials: E
authors.lastname: Säwén
authors.forename: Jennie
authors.initials: J
authors.lastname: Östervall
authors.forename: Clas
authors.initials: C
authors.lastname: Landersjö
authors.forename: Malin
authors.initials: M
authors.lastname: Edblad
authors.forename: Andrej
authors.initials: A
authors.lastname: Weintraub
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ansaruzzaman
authors.forename: Göran
authors.initials: G
authors.lastname: Widmalm
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D016981:Plesiomonas / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019081:O Antigens