title: High-resolution view of the yeast meiotic program revealed by ribosome profiling.
abstract: Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5' leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.
authors.affiliation: Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
authors.forename: Gloria A
authors.initials: GA
authors.lastname: Brar
authors.forename: Moran
authors.initials: M
authors.lastname: Yassour
authors.forename: Nir
authors.initials: N
authors.lastname: Friedman
authors.forename: Aviv
authors.initials: A
authors.lastname: Regev
authors.forename: Nicholas T
authors.initials: NT
authors.lastname: Ingolia
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Weissman
mesh_terms: D020121:5' Untranslated Regions; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D059014:High-Throughput Nucleotide Sequencing; D008540:Meiosis; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D013172:Spores, Fungal / Q000502:physiology
chemical_list: D020121:5' Untranslated Regions; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins