title: New rhodamine nitroxide based fluorescent probes for intracellular hydroxyl radical identification in living cells.
abstract: The synthesis, characteristics, and biological applications of a series of new rhodamine nitroxide fluorescent probes that enable imaging of hydroxyl radicals (•OH) in living cells are described. These probes are highly selective for •OH in aqueous solution, avoiding interference from other reactive oxygen species (ROS), and they facilitate •OH imaging in biologically active samples. The robust nature of these probes (high specificity and selectivity, and facile synthesis) offer distinct advantages over previous methods for •OH detection.
authors.affiliation: Department of Chemistry and Department of Biological Sciences, Michigan Technological University, Houghton, Michigan 49931, USA.
authors.forename: Nazmiye B
authors.initials: NB
authors.lastname: Yapici
authors.forename: Steffen
authors.initials: S
authors.lastname: Jockusch
authors.forename: Alberto
authors.initials: A
authors.lastname: Moscatelli
authors.forename: Srinivas Rao
authors.initials: SR
authors.lastname: Mandalapu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Itagaki
authors.forename: Dallas K
authors.initials: DK
authors.lastname: Bates
authors.forename: Sherri
authors.initials: S
authors.lastname: Wiseman
authors.forename: K Michael
authors.initials: KM
authors.lastname: Gibson
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: Lanrong
authors.initials: L
authors.lastname: Bi
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis* / Q000737:chemistry; D042541:Intracellular Space / Q000737:chemistry*; D015394:Molecular Structure; D009589:Nitrogen Oxides / Q000032:analysis* / Q000737:chemistry; D018384:Oxidative Stress; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009589:Nitrogen Oxides; D012235:Rhodamines; D017665:Hydroxyl Radical