title: Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison.
abstract: Violacein and deoxyviolacein display a broad range of interesting biological properties but their production is rarely distinguished due to the lack of suitable analytical methods. An HPLC method has been developed for the separation and quantification of violacein and deoxyviolacein and can determine the content of both molecules in microbial cultures. A comparison of different production microorganisms, including recombinant Escherichia coli and the natural producer Janthinobacterium lividum, revealed that the formation of violacein and deoxyviolacein is strain-specific but showed significant variation during growth although the ratio between the two compounds remained constant.
authors.affiliation: Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaußstrasse 17, 38106, Braunschweig, Germany.
authors.forename: André L
authors.initials: AL
authors.lastname: Rodrigues
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Göcke
authors.forename: Christoph
authors.initials: C
authors.lastname: Bolten
authors.forename: Nelson L
authors.initials: NL
authors.lastname: Brock
authors.forename: Jeroen S
authors.initials: JS
authors.lastname: Dickschat
authors.forename: Christoph
authors.initials: C
authors.lastname: Wittmann
mesh_terms: D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D007211:Indoles / Q000302:isolation & purification / Q000378:metabolism*; D042802:Oxalobacteraceae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001688:Biological Products; D007211:Indoles; C063155:violacein