title: Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine.
abstract: The rapid growth of the global HIV/AIDS epidemic makes it a high priority to develop an effective vaccine. Since a live attenuated or inactivated HIV vaccine is not likely to be approved for clinical application due to safety concerns, HIV virus like particles (VLPs) offer an attractive alternative because they are considered safer since they lack viral genome. We got a stable eukaryotic cell line by G418 resistance selection, engineered to express the HIV-1 structure protein Gag and Env efficiently and stably. We confirmed the presence of Gag and Env proteins in the cell culture supernatant and that they could self-assemble into VLPs. These VLPs were found to be able to elicit specific humoral and cellular immune response after immunization without any adjuvant.
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: XiZhen
authors.initials: X
authors.lastname: Zhang
authors.forename: XiaoDan
authors.initials: X
authors.lastname: Wang
authors.forename: DongHai
authors.initials: D
authors.lastname: Zhao
authors.forename: XiangYu
authors.initials: X
authors.lastname: Meng
authors.forename: XingHong
authors.initials: X
authors.lastname: Zhao
authors.forename: XiangHui
authors.initials: X
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D005260:Female; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D057809:HEK293 Cells; D015483:HIV Antibodies / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000276:immunology; D014771:Virion / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D058425:Vaccines, Virus-Like Particle; D007371:Interferon-gamma