title: Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels.
abstract: We have developed a method to analyze the substrate transport of ATP-binding cassette (ABC) transporters, which are associated with drug resistance in tumor cells. Our microfluidic method is well suited to the single-vesicle estimation of substrate transport and the rapid drug screening of ABC transporters. Using this method, we have demonstrated, for the first time, the analysis of substrate transport by a single transporter and performed drug-inhibition experiments in less than 3 h.
authors.affiliation: Kanagawa Academy of Science and Technology, Kawasaki, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Sasaki
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kawano
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Osaki
authors.forename: Koki
authors.initials: K
authors.lastname: Kamiya
authors.forename: Shoji
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D009369:Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000378:metabolism*
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008081:Liposomes