title: Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system.
abstract: ComX pheromone is an isoprenoidal oligopeptide containing a modified tryptophan residue, which stimulates natural genetic competence in the gram-positive bacterium Bacillus. Since posttranslational prenylation on the tryptophan residue has not been reported except in ComX pheromone, the universality of this modification has not yet been elucidated. In this paper, we established a cell-free system, whereby the tryptophan residue in peptides is modified with a geranyl group by modifying enzyme ComQ. In addition, we investigated enzymatic reaction conditions using an in vitro enzyme reaction system. This is the first report of in vitro geranylation on the tryptophan residue. This system is potentially a useful tool for elucidating the universality of prenylation on the tryptophan residue.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
authors.forename: Fumitada
authors.initials: F
authors.lastname: Tsuji
authors.forename: Ayako
authors.initials: A
authors.lastname: Ishihara
authors.forename: Keiko
authors.initials: K
authors.lastname: Kurata
authors.forename: Aya
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kitamura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kanamaru
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Masuda
authors.forename: Kazuma
authors.initials: K
authors.lastname: Murakami
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Irie
authors.forename: Youji
authors.initials: Y
authors.lastname: Sakagami
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D002474:Cell-Free System; D051228:Geranyltranstransferase / Q000378:metabolism*; D066298:In Vitro Techniques; D008954:Models, Biological; D008969:Molecular Sequence Data; D010675:Pheromones / Q000737:chemistry / Q000378:metabolism; D017368:Protein Prenylation / Q000502:physiology; D011499:Protein Processing, Post-Translational; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C087040:ComX protein, Bacillus subtilis; D010675:Pheromones; D014364:Tryptophan; D051228:Geranyltranstransferase