title: [Influence of storage conditions on results of antibody level assay in human serum samples].
abstract: Human serum samples containing immunoglobulin G (IgG) and M (1gM) were stored at 4 degrees C, and -20 degrees C with and without thaw/freeze cycles by 30 days. Anti-HAV IgG, anti-EBV IgG and anti-EBV IgM were investigated by ELISA and ELFA methods. Freezing have given significant growth of measured antibodies concentration. In the IgM case, significant loose of activity was observed followed its growth in all assay conditions. Anti EBV IgG stored at freezing conditions were much stable than stored at 4 degrees C. Greatest changes of antibodies activity was found after multiple thawing and freezing.
authors.affiliation: Wojewódzka Stacja Sanitarno-Epidemiologiczna w Lublinie.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Truszkiewicz
mesh_terms: D001793:Blood Preservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D044967:Serum / Q000276:immunology*; D013048:Specimen Handling / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M