title: Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803.
abstract: The cyanobacterium, Synechocystis sp. PCC 6803, was the first photosynthetic organism whose genome sequence was determined in 1996 (Kazusa strain). It thus plays an important role in basic research on the mechanism, evolution, and molecular genetics of the photosynthetic machinery. There are many substrains or laboratory strains derived from the original Berkeley strain including glucose-tolerant (GT) strains. To establish reliable genomic sequence data of this cyanobacterium, we performed resequencing of the genomes of three substrains (GT-I, PCC-P, and PCC-N) and compared the data obtained with those of the original Kazusa strain stored in the public database. We found that each substrain has sequence differences some of which are likely to reflect specific mutations that may contribute to its altered phenotype. Our resequence data of the PCC substrains along with the proposed corrections/refinements of the sequence data for the Kazusa strain and its derivatives are expected to contribute to investigations of the evolutionary events in the photosynthetic and related systems that have occurred in Synechocystis as well as in other cyanobacteria.
authors.affiliation: Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.forename: Yuh
authors.initials: Y
authors.lastname: Shiwa
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tajima
authors.forename: Marie
authors.initials: M
authors.lastname: Suzuki
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Naoki
authors.initials: N
authors.lastname: Sato
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D004252:DNA Mutational Analysis; D030541:Databases, Genetic; D056426:Genetic Loci; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D054643:INDEL Mutation; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D046939:Synechocystis / Q000145:classification / Q000235:genetics*