title: Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells.
abstract: Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.
authors.affiliation: Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749 Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kwak
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Jin
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Ryu
authors.forename: Kinram
authors.initials: K
authors.lastname: Ko
authors.forename: So-Dam
authors.initials: SD
authors.lastname: Lee
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Kyu Yong
authors.initials: KY
authors.lastname: Jung
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hwang
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D005732:Gangliosides / Q000378:metabolism*; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism
chemical_list: D005732:Gangliosides; C039129:GQ1b ganglioside; C526452:ST8SiaII protein, mouse; D012799:Sialyltransferases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3