title: Endocrine disrupting chemicals affect the adipogenic differentiation of mesenchymal stem cells in distinct ontogenetic windows.
abstract: Endocrine disrupting chemicals (EDC) like bisphenol A (BPA), bis(2-ethylhexyl)phthalate (DEHP) and tributyltin (TBT) are ubiquitously present in the environment and in human tissues. They bind to nuclear hormone receptors and affect cellular and developmental processes. In this study, we show that BPA, DEHP and TBT affect the adipogenic differentiation of murine mesenchymal stem cells (MSC, C3H/10T1/2) in a concentration-, stage- and compound-specific manner. C3H/10T1/2 cells and embryonic stem cells (CGR8) were exposed to BPA, DEHP or TBT at different stages of cell determination and differentiation (undifferentiated growth, adipogenic induction and terminal adipogenic differentiation). The final amount of differentiated adipocytes, cellular triglyceride content and mRNA expression of adipogenic marker genes (adiponectin, FABP4, PPARγ2, LPL) were quantified and compared with corresponding unexposed cells. BPA (10 μM) decreased subsequent adipogenic differentiation of MSC, when cells were exposed during undifferentiated growth. In contrast, DEHP (100 μM) during the hormonal induction period, and TBT (100 nM) in all investigated stages, enhanced adipogenesis. Importantly, exposure of undifferentiated murine embryonic stem cells did not show any effect of the investigated EDC on subsequent adipogenic differentiation.
authors.affiliation: Department of Anatomy and Cell Biology, Martin Luther University, Faculty of Medicine, Halle, Germany. ronald.biemann@medizin.uni-halle.de
authors.forename: Ronald
authors.initials: R
authors.lastname: Biemann
authors.forename: Anne
authors.initials: A
authors.lastname: Navarrete Santos
authors.forename: Alexander
authors.initials: A
authors.lastname: Navarrete Santos
authors.forename: Dagmar
authors.initials: D
authors.lastname: Riemann
authors.forename: Julia
authors.initials: J
authors.lastname: Knelangen
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
authors.forename: Holger
authors.initials: H
authors.lastname: Koch
authors.forename: Bernd
authors.initials: B
authors.lastname: Fischer
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001559:Benzhydryl Compounds; D002460:Cell Line; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D010636:Phenols / Q000494:pharmacology*; D014220:Trialkyltin Compounds / Q000494:pharmacology*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004051:Diethylhexyl Phthalate; D052244:Endocrine Disruptors; D010636:Phenols; D014220:Trialkyltin Compounds; C011559:tributyltin; C006780:bisphenol A; D000098826:Bisphenol A Compounds