title: Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
abstract: We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.
authors.affiliation: Centre of Marine Sciences, University of Algarve, Faro, Portugal. jvarela@ualg.pt.
authors.forename: Hugo
authors.initials: H
authors.lastname: Pereira
authors.forename: Luísa
authors.initials: L
authors.lastname: Barreira
authors.forename: André
authors.initials: A
authors.lastname: Mozes
authors.forename: Cláudia
authors.initials: C
authors.lastname: Florindo
authors.forename: Cristina
authors.initials: C
authors.lastname: Polo
authors.forename: Catarina V
authors.initials: CV
authors.lastname: Duarte
authors.forename: Luísa
authors.initials: L
authors.lastname: Custódio
authors.forename: João
authors.initials: J
authors.lastname: Varela