title: IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis.
abstract: Nasal polyps in adults are characterized by a chronic inflammation of the upper airways and by the preferential activation of Th2 cells. In contrast, IL-17 producing Th17 cells dominate the inflammation in nasal polyps of cystic fibrosis (CF) patients.
  
  
  METHODS
  IL-17A, IL-5, IL-6, IL-8, IL-1β, ECP, MCP-1 and myeloperoxidase expression was determined in tissue homogenates of nasal polyps of non-CF and CF patients and controls. The cellular source of IL-17A was determined by immuno-histochemistry and FACS analysis. The functional role of IL-17A in the survival of neutrophils from CF and non-CF patients was tested.
  
  
  RESULTS
  A significant upregulation of IL-17A and myeloperoxidase could be observed in nasal polyps from CF-patients. The cellular sources of IL-17A in nasal polyps were mainly T-lymphocytes. IL-17A was able to modulate the survival of neutrophils in nasal polyps from non-CF patients; however the survival of neutrophils in CF patients was independent of IL-17A.
  
  
  CONCLUSIONS
  The present study shows that IL-17A has an impact on neutrophil survival in adult nasal polyp disease, but not in nasal polyps from CF patients.
authors.affiliation: Upper Airway Research Laboratory (URL), Department of Oto-Rhino-Laryngology, Ghent University Hospital, Ghent University, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Derycke
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Holtappels
authors.forename: T
authors.initials: T
authors.lastname: Dutré
authors.forename: C
authors.initials: C
authors.lastname: Bachert
mesh_terms: D000328:Adult; D002470:Cell Survival; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D009298:Nasal Polyps / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism; D058504:Th17 Cells / Q000378:metabolism
chemical_list: D020381:Interleukin-17