title: Investigation of autoantibody profiles for cerebrospinal fluid biomarker discovery in patients with relapsing-remitting multiple sclerosis.
abstract: Using the UNIarray® marker technology platform, cerebrospinal fluid immunoglobulin G reactivities of 15 controls and 17 RRMS patients against human recombinant proteins were investigated. Patient cerebrospinal fluids were oligoclonal band positive and reactivities were compared to that of sex- and age-matched controls. We hereby aimed at the characterization of autoreactivity in patients with RRMS. Differences in autoreactivities between control and RRMS samples were identified comprising autoantigens identified in this study only and previously reported autoantigens as well. A combination of the 10-15 most significant proteins may be investigated further as autoantigens for diagnostic purposes. Additional investigations may include minimizing the number of proteins used in such diagnostic tests.
authors.affiliation: Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. nhbeyer@gmail.com
authors.forename: Natascha Helena
authors.initials: NH
authors.lastname: Beyer
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Axel
authors.initials: A
authors.lastname: Kowald
authors.forename: Jette Lautrup
authors.initials: JL
authors.lastname: Frederiksen
authors.forename: Niels H H
authors.initials: NH
authors.lastname: Heegaard
mesh_terms: D000328:Adult; D000368:Aged; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000096:biosynthesis* / Q000378:metabolism; D001324:Autoantigens / Q000276:immunology / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology*; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; D007074:Immunoglobulin G; D019151:Peptide Library