Spatial manipulation of cells and organelles using single electrode dielectrophoresis.
The selection, isolation, and accurate positioning of single cells in three dimensions are increasingly desirable in many areas of cell biology and tissue engineering. We describe the application of a simple and low cost dielectrophoretic device for picking out and relocating single target cells. The device consists of a single metal electrode and an AC signal generator. It does not require microfabrication technologies or sophisticated electronics. The dielectrophoretic manipulator also discriminates between live and dead cells and is capable of redistributing intracellular organelles.
The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Woods Hole, MA, USA. dgraham@mbl.edu
David M
DM
Graham
Mark A
MA
Messerli
Ronald
R
Pethig
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006367:HeLa Cells; D006801:Humans; D008839:Microelectrodes; D015388:Organelles; D059010:Single-Cell Analysis / Q000379:methods*