[Study on enriching total flavonoids from folium chrysanthemi with polyamide and macroporous resin].
OBJECTIVE
To study the technology of enriching total flavonoids from Folium Chrysanthemi with polyamide and macroporous resin.


METHODS
By using the content of flavonoids as index, firstly absorbed with polyamide and removed impurity with purified water. Then added polyamide to macroporous resin column, eluted with ethanol and determined the flavonoids content.


RESULTS
Total flavonoids could be effectively purified by the polyamide and macroporous resin. Best technological conditions were as follows: resin of type X-5 was better, and elution agent was 70% ethanol, the ratio of extract amount (mL) and polyamide quantity (g) was 1. 5: 1, ratio of resin (g) - extract (mL) was 4: 3, ratio of diameter and height was 1: 20, the volume of collected eluent was 2BV. The purity of total flavonoids reached to 87.48%.


CONCLUSIONS
The technology is stable and can be viewed as a way to study and develop the resources of Folium Chrysanthemi.
Pharmacology College, Anhui Xinhua University, Hefei 230088, China. weiqiang509@sina.com
Qiang
Q
Wei
Yi
Y
Dai
Yan
Y
Wu
Wen-Jing
WJ
Zhang
D000042:Absorption; D019659:Asteraceae / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification*; D009757:Nylons / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods
D005419:Flavonoids; D009757:Nylons; D012117:Resins, Synthetic