Backbone and side chain 1H, 13C and 15N resonance assignments of the catalytic domain of diphtheria toxin.
Diphtheria is a serious upper respiratory tract disease caused by the diphtheria toxin (DT) secreted from the bacteria Corynebacterium diphtheriae. Vaccination is the best way to protect against this infectious disease. Diphtheria vaccines are prepared by isolating, purifying and chemically deactivating DT. Although toxoids have been used for decades in immunization, there is still little understanding at the molecular level of the process of toxoid preparation, and how chemical treatment enhances their immunogenicity. We have undertaken an NMR study of the catalytic domain as a first step in understanding the molecular details involved in vaccine antigen preparation. Here we report a near complete assignment for the backbone and side chain resonances of the diphtheria toxin catalytic domain.
Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, Health Canada, 251 Sir Frederick Banting Driveway, A/L 2201E, Tunney's Pasture, Ottawa, ON K1A 0K9, Canada.
Simon
S
Sauvé
Geneviève
G
Gingras
Yves
Y
Aubin
D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D020134:Catalytic Domain; D003353:Corynebacterium diphtheriae / Q000378:metabolism*; D004167:Diphtheria Toxin / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011522:Protons
D002247:Carbon Isotopes; D004167:Diphtheria Toxin; D009587:Nitrogen Isotopes; D011522:Protons