First evidence on different transportation modes of arsenic and phosphorus in arsenic hyperaccumulator Pteris vittata.
Arsenic (As) reduction and translocation are key processes for As hyperaccumulation by the hyperaccumulator Pteris vittata L. Micro-X-ray adsorption spectroscopy of P. vittata's rhizoid tissues revealed that As reduction mainly occurred in endodermis during translocation from epidermis to vascular bundle. Prior to reduction, arsenate (As (V)) translocation was an active process requiring energy and employing a phosphate (P) transporter. Use of a synchrotron X-ray microprobe showed that As (V) and P were cotransported and that this process could be enhanced by As (V) exposure or P deficiency but restrained by energy release inhibition caused by 2,4-dinitrophenol or sodium orthovanadate. In contrast, after As reduction, As(III) translocation differed from P translocation and was more efficient, appearing free from the apparent endodermal blockage. The results here revealed the role of the P transporter on As translocation as well as the key role of As reduction in As hyperaccumulation by P. vittata.
Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.
Mei
M
Lei
Xiao-Ming
XM
Wan
Ze-Chun
ZC
Huang
Tong-Bin
TB
Chen
Xue-Wen
XW
Li
Ying-Ru
YR
Liu
D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008956:Models, Chemical; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D032495:Pteris / Q000737:chemistry / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D056928:X-Ray Absorption Spectroscopy
D012989:Soil Pollutants; D010758:Phosphorus; D001151:Arsenic