Analgesic and anti-inflammatory activity of β-sitosterol isolated from Nyctanthes arbortristis leaves.
Nyctanthes arbortristis Linn. (Oleaceae) is one of the well-known Indian medicinal plant. Various extracts of leaves of the plant were screened for analgesic activity by hot plate test and acetic acid-induced writhings and anti-inflammatory activity by carrageenan-induced hind paw edema method at the dose of 50 mg/kg, i.p. Petroleum ether extract was found to be most active and hence subjected to activity-guided fractionation. Results showed that β-sitosterol (5, 10 and 20 mg/kg, i.p.) was responsible for the significant and dose-dependent activity comparable with the standard extract. β-Sitosterol from N. arbortristis leaves might be responsible for analgesic and anti-inflammatory activity.
Department of Pharmacognosy, Pravara Rural College of Pharmacy, Pravaranagar, Maharashtra, India. nirmalsunil@rediffmail.com
Sunil A
SA
Nirmal
Subodh C
SC
Pal
Subhash C
SC
Mandal
Anuja N
AN
Patil
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D007194:India; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D029592:Oleaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012855:Sitosterols; C025473:gamma-sitosterol