Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo.
Serotonergic neurons differentiate in the neurogenic animal plate ectoderm of the sea urchin embryo. The regulatory mechanisms that control the specification or differentiation of these neurons in the sea urchin embryo are not yet understood, although, after the genome was sequenced, many genes encoding transcription factors expressed in this region were identified. Here, we report that zinc finger homeobox (zfhx1/z81) is expressed in serotonergic neural precursor cells, using double in situ hybridization screening with a serotonergic neural marker, tryptophan 5-hydroxylase (tph) encoding a serotonin synthase that is required for the differentiation of serotonergic neurons. zfhx1/z81 begins to be expressed at gastrula stage in individual cells in the anterior neuroectoderm, some of which also express delta. zfhx1/z81 expression gradually disappears as neural differentiation begins with tph expression. When the translation of Zfhx1/Z81 is blocked by morpholino injection, embryos express neither tph nor the neural marker synaptotagminB in cells of the animal plate, and serotonergic neurons do not differentiate. In contrast, Zfhx1/Z81 morphants do express fez, another neural precursor marker, which appears to function in the initial phase of specification/differentiation of serotonergic neurons. In addition, zfhx1/z81 is one of the targets suppressed in the animal plate by anti-neural signals such as Nodal as well as Delta-Notch. We conclude that Zfhx1/Z81 functions during the specification of individual anterior neural precursors and promotes the expression of tph and synaptotagminB, required for the differentiation of serotonergic neurons.
Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan.
Junko
J
Yaguchi
Lynne M
LM
Angerer
Kazuo
K
Inaba
Shunsuke
S
Yaguchi
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005775:Gastrula / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D005801:Genes, Homeobox / Q000235:genetics*; D047330:Hemicentrotus / Q000196:embryology / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D055457:Nodal Protein / Q000235:genetics; D051880:Receptors, Notch / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D059326:Serotonergic Neurons / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D050857:Synaptotagmins / Q000235:genetics; D014365:Tryptophan Hydroxylase / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics*
D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D055457:Nodal Protein; D051880:Receptors, Notch; C064045:delta protein; D050857:Synaptotagmins; D014365:Tryptophan Hydroxylase