Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma.
BACKGROUND
Altered deposition of extracellular matrix (ECM) in the airway smooth muscle (ASM) layer as observed in asthma may influence ASM mechanical properties. We hypothesized that ECM in ASM is associated with airway function in asthma. First, we investigated the difference in ECM expression in ASM between asthma and controls. Second, we examined whether ECM expression is associated with bronchoconstriction and bronchodilation in vivo.


METHODS
Our cross-sectional study comprised 19 atopic mild asthma patients, 15 atopic and 12 nonatopic healthy subjects. Spirometry, methacholine responsiveness, deep-breath-induced bronchodilation (ΔR(rs) ) and bronchoscopy with endobronchial biopsies were performed. Positive staining of elastin, collagen I, III and IV, decorin, versican, fibronectin, laminin and tenascin in ASM was quantified as fractional area and mean density. Data were analysed using Pearson's or Spearman's correlation coefficient.


RESULTS
Extracellular matrix expression in ASM was not different between asthma and controls. In asthmatics, fractional area and mean density of collagen I and III were correlated with methacholine dose-response slope and ΔR(rs) , respectively (r = 0.71, P < 0.01; r = 0.60, P = 0.02). Furthermore, ASM collagen III and laminin in asthma were correlated with FEV(1)  reversibility (r = -0.65, P = 0.01; r = -0.54, P = 0.04).


CONCLUSIONS
In asthma, ECM in ASM is related to the dynamics of airway function in the absence of differences in ECM expression between asthma and controls. This indicates that the ASM layer in its full composition is a major structural component in determining variable airways obstruction in asthma.
Department of Respiratory Medicine, Academic Medical Centre, Amsterdam, the Netherlands. c.y.yick@amc.uva.nl
C Y
CY
Yick
D S
DS
Ferreira
R
R
Annoni
J H
JH
von der Thüsen
P W
PW
Kunst
E H
EH
Bel
R
R
Lutter
T
T
Mauad
P J
PJ
Sterk
D000328:Adult; D001249:Asthma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D001980:Bronchi / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D001999:Bronchoscopy; D003094:Collagen / Q000378:metabolism; D003430:Cross-Sectional Studies; D005109:Extracellular Matrix / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000378:metabolism* / Q000473:pathology; D012129:Respiratory Function Tests; D055815:Young Adult
D003094:Collagen