Hho1p, the linker histone of Saccharomyces cerevisiae, is important for the proper chromatin organization in vivo.
Despite the existence of certain differences between yeast and higher eukaryotic cells a considerable part of our knowledge on chromatin structure and function has been obtained by experimenting on Saccharomyces cerevisiae. One of the peculiarities of S. cerevisiae cells is the unusual and less abundant linker histone, Hho1p. Sparse is the information about Hho1p involvement in yeast higher-order chromatin organization. In an attempt to search for possible effects of Hho1p on the global organization of chromatin, we have applied Chromatin Comet Assay (ChCA) on HHO1 knock-out yeast cells. The results showed that the mutant cells exhibited highly distorted higher-order chromatin organization. Characteristically, linker histone depleted chromatin generally exhibited longer chromatin loops than the wild-type. According to the Atomic force microscopy data the wild-type chromatin appeared well organized in structures resembling quite a lot the "30-nm" fiber in contrast to HHO1 knock-out yeast.
Laboratory of Yeast Molecular Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Milena
M
Georgieva
Assen
A
Roguev
Konstantin
K
Balashev
Jordanka
J
Zlatanova
George
G
Miloshev
D002843:Chromatin / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D006657:Histones / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D009154:Mutation; D009707:Nucleosomes / Q000235:genetics / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D014158:Transcription, Genetic
D002843:Chromatin; C480035:HHO1 protein, S cerevisiae; D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins