Matrix-based yeast two-hybrid screen strategies and comparison of systems.
Today, matrix-based screens are used primarily for smaller and medium-size clone collections in combination with automation and cloning techniques that allow for reliable and fast interaction screening. Matrix-based yeast two-hybrid screens are an alternative to library-based screens. However, intermediary forms are possible too and we compare both strategies, including a detailed discussion of matrix-based screens. Recent improvement of matrix screens (also called array screens) uses various pooling strategies as well as novel vectors that increase their efficiency while decreasing false-negative rates and increasing reliability.
Karlsruhe Institute of Technology, Karlsruhe, Germany.
Roman
R
Häuser
Thorsten
T
Stellberger
Seesandra V
SV
Rajagopala
Peter
P
Uetz
D004867:Equipment Design; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques / Q000191:economics / Q000295:instrumentation
D011993:Recombinant Fusion Proteins