Constituents of Dalbergia sissoo Roxb. leaves with osteogenic activity.
One new isoflavone glucoside, caviunin 7-O-[β-d-apiofuranosyl-(1→6)-β-d-glucopyranoside] (10) and a new itaconic derivative, (E)-4-methoxy-2-(3,4-dihydroxybenzylidene)-4-oxobutanoic acid (15) along with series of isoflavones and flavonols with their glucosides (1-9 and 11-14) and a lignan glucoside (16) were isolated from the ethanolic extract of Dalbergia sissoo leaves. The structures of these compounds were established on the basis of IR, UV, (1)H and (13)C NMR, DEPT, COSY, HSQC, HMBC and MS data. All compounds (1-16) were assessed for osteogenic activity in primary calvarial osteoblast cultures. Compounds 1-4 and 10 increased alkaline phosphatase activity and mineralization thus resulting in significant osteogenic activity.
Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226001, India.
Preety
P
Dixit
Raju
R
Chillara
Vikram
V
Khedgikar
Jyoti
J
Gautam
Priyanka
P
Kushwaha
Avinash
A
Kumar
Divya
D
Singh
Ritu
R
Trivedi
Rakesh
R
Maurya
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D035782:Dalbergia / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D061251:Primary Cell Culture; D051381:Rats; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D010936:Plant Extracts