Real-time nucleic acid sequence-based amplification to predict the clinical outcome of invasive aspergillosis.
Monitoring the response to therapy for invasive aspergillosis (IA) is essential for the management of patients with hematologic diseases. We evaluated the correlation between the outcome of real-time nucleic acid sequence-based amplification (RTi-NASBA) for Aspergillus 18S rRNA and the clinical outcome of IA. A total of 157 serum samples from 29 patients with IA were tested for RTi-NASBA. The treatment response and mortality were compared with the NASBA outcome (whether the NASBA value was converted to negative or not) at 12 weeks after the start of antifungal therapy. At 12 weeks, there was a moderate correlation between the treatment failure and persistently positive NASBA (κ = 0.482; P = 0.019). Deaths attributable to IA were more prevalent in patients without negative conversion of NASBA than in those with negative conversion (50% vs 5%; P = 0.013). Significant factors of treatment failure at 12 weeks were the status of hematologic disease (nonremission; P = 0.041) and the NASBA outcome (failure of negative conversion; P = 0.024). Survival was significantly better in patients with negative conversion of NASBA than those with persistently positive values (P = 0.036). This study suggests that the serial monitoring of RTi-NASBA could be useful for prediction of the clinical outcome in hematologic patients with IA.
Division of Infectious Diseases, Department of Internal Medicine, The Catholic University of Korea College of Medicine, Seoul, Korea.
Si-Hyun
SH
Kim
Chulmin
C
Park
Eun-Young
EY
Kwon
Na-Young
NY
Shin
Jae-Cheol
JC
Kwon
Sun Hee
SH
Park
Su-Mi
SM
Choi
Dong-Gun
DG
Lee
Jung-Hyun
JH
Choi
Jin-Hong
JH
Yoo
D000293:Adolescent; D000328:Adult; D000368:Aged; D000935:Antifungal Agents / Q000627:therapeutic use; D001228:Aspergillosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000401:mortality; D001230:Aspergillus / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D005260:Female; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D013183:Sputum / Q000382:microbiology; D015996:Survival Rate
D000935:Antifungal Agents; D012337:RNA, Ribosomal, 18S