Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP10.
Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infecting P. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan.
Jumpei
J
Uchiyama
Mohammad
M
Rashel
Iyo
I
Takemura
Shin-Ichiro
S
Kato
Takako
T
Ujihara
Asako
A
Muraoka
Shigenobu
S
Matsuzaki
Masanori
M
Daibata
D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008242:Lysogeny; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D037521:Virulence Factors / Q000235:genetics
D004279:DNA, Viral; D037521:Virulence Factors; D012343:RNA, Transfer