Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo.
Pyrrolysine-tRNA(Pyl) complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA(Pyl) in vitro. In addition, the PylRS/tRNA(Pyl) pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA(Pyl) pair can be used in protein engineering technology.
Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan. katay@tokai-u.jp
Hidekazu
H
Katayama
Kayo
K
Nozawa
Osamu
O
Nureki
Yoshiaki
Y
Nakahara
Hironobu
H
Hojo
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism*; D045858:Desulfitobacterium / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D013379:Substrate Specificity
D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine