Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus  spp.) in Peru.
In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.
International Potato Center, Apartado, 1558, Lima 12, Peru.
Segundo
S
Fuentes
Bettina
B
Heider
Ruby Carolina
RC
Tasso
Elisa
E
Romero
Thomas
T
Zum Felde
Jan Frederik
JF
Kreuze
D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D029905:Pachyrhizus / Q000821:virology*; D010568:Peru; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification*; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D034741:RNA, Small Interfering; D012367:RNA, Viral