Decreased pre-surgical CD34+/CD144+ cell number in patients undergoing coronary artery bypass grafting compared to coronary artery disease-free valvular patients.
BACKGROUND
Cardiovascular disease has been linked to endothelial progenitor cell (EPC) depletion and functional impairment in atherosclerosis and aortic stenosis. EPCs may play a pivotal role in vascular grafting. However, the EPC depletion in coronary artery bypass grafting (CABG) patients has not been compared to coronary artery disease-free valvular replacement patients with aortic stenosis.


METHODS
We aimed to assess the basal number of CD34+/KDR+ and CD34+/CD144+ cells in CABG patients, compared to aortic stenosis valvular replacement patients. 100 patients (51 CABG and 49 valvular surgery ones) were included in the present study. All CABG or valvular patients had angiographic demonstration of the presence or the absence of coronary artery disease, respectively. Numbers of CD34+/KDR+ and CD34+/CD144+ were assessed by flow cytometry of pre-surgical blood samples.


RESULTS
We found a lower number of CD34+/CD144+ cells in CABG patients compared to valvular patients (0.21 ± 0.03% vs. 0.47 ± 0.08%), and this difference remained statistically significant after the P was adjusted for multiple comparisons (P = 0.01428). Both groups had more EPCs than healthy controls.


CONCLUSIONS
Pre-surgical CD34+/CD144+ numbers are decreased in CABG patients, compared to valvular patients with absence of coronary disease.
Department of Pharmacology, School of Medicine, Universidad Complutense, Madrid, Spain. santiredondo@hotmail.com
Santiago
S
Redondo
Alvaro
A
González-Rocafort
Jorge
J
Navarro-Dorado
Marta
M
Ramajo
Mihail
M
Hristov
Antonio
A
Gordillo-Moscoso
Fernando
F
Reguillo
Manuel
M
Carnero
Jose
J
Martinez-Gonzalez
Enrique
E
Rodríguez
Christian
C
Weber
Teresa
T
Tejerina
D000368:Aged; D015703:Antigens, CD / Q000097:blood / Q000276:immunology*; D018952:Antigens, CD34 / Q000097:blood / Q000276:immunology*; D001024:Aortic Valve Stenosis / Q000150:complications* / Q000276:immunology / Q000601:surgery; D015820:Cadherins / Q000097:blood / Q000276:immunology*; D002452:Cell Count; D017023:Coronary Angiography; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000150:complications / Q000276:immunology* / Q000601:surgery; D003331:Coronary Vessels / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D004730:Endothelium, Vascular / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D057234:Preoperative Period; D000099152:Cadherin 5
D015703:Antigens, CD; D018952:Antigens, CD34; D015820:Cadherins; D000099152:Cadherin 5