Arthrobacter cupressi sp. nov., an actinomycete isolated from the rhizosphere soil of Cupressus sempervirens.
An actinobacterial strain, designated D48(T), was isolated from the rhizosphere soil of a cypress tree collected from Mianyang in Sichuan province, China. The strain was Gram-stain-positive, catalase-positive, oxidase-negative and non-motile, with lysine as the peptidoglycan diagnostic diamino acid and acetyl as the peptidoglycan acyl type. The predominant menaquinone was MK-9(H(2)); small amounts of MK-7(H(2)), MK-10(H(2)) and MK-6 were also present. The major fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(16:0). The isolate underwent a rod-coccus morphological cycle, had a high DNA G+C content, was aerobic and grew between 12 and 37 °C (optimum, 28 °C). On the basis of the phenotypic and chemotaxonomic analyses, 16S rRNA gene sequence comparisons and DNA-DNA hybridization data, the isolate represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cupressi sp. nov. is proposed. The type strain is D48(T) (=DSM 24664(T)=CGMCC 1.10783(T)).
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
Jian
J
Zhang
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, People's Republic of China.|Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
Yuchao
Y
Ma
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China.
Huimin
H
Yu
D001173:Arthrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D029781:Cupressus / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2