Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats.
Effects of hindlimb suspension (HS) and ambulation recovery on hippocampal neurogenesis of newly weaned rats were studied by using immunohistochemical techniques. The number of proliferating cell nuclear antigen-positive (PCNA(+)) cells in the subgranular zone (SGZ) markedly decreased during normal growth. However, neither HS nor subsequent recovery caused additional changes in the number of PCNA(+) cells. The number of doublecortin-positive (DCX(+)) neurons decreased gradually during normal growth. HS resulted in a further decrease in these neurons. However, DCX(+) cell numbers became identical to the levels in age-matched controls after 14 days of recovery. PCNA and DCX-double positive cells in the SGZ were also observed, and their cell numbers were not affected by HS and 14-day ambulation. Thus, HS suppressed the generation of DCX(+) neurons without affecting PCNA(+) cells in the SGZ of weaned rats. Taken together, hippocampal neurogenesis in weaned rats was not severely affected by HS while it decreased significantly as they had grown.
Graduate School of Medicine, Osaka University, Toyonaka City, Osaka 560-0043, Japan.
Sachiko
S
Nomura
Katsuya
K
Kami
Fuminori
F
Kawano
Yoshihiko
Y
Oke
Naoya
N
Nakai
Takashi
T
Ohira
Ryo
R
Fujita
Masahiro
M
Terada
Kazuhiko
K
Imaizumi
Yoshinobu
Y
Ohira
D000818:Animals; D001835:Body Weight; D002452:Cell Count; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology; D000089662:Doublecortin Protein; D019417:Hindlimb Suspension / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology*; D006728:Hormones / Q000097:blood / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000502:physiology; D055495:Neurogenesis; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D014886:Weaning
C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D006728:Hormones; D036341:Intercellular Signaling Peptides and Proteins