Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses.
Although a physiological role of heat-shock proteins (HSP) in antigen presentation and immune response activation has not been directly demonstrated, their use as vaccine components is under clinical trial. We have previously demonstrated that the structure of plant-derived HSP70 (pHSP70) can be superimposed to the mammalian homologue and similarly to the mammalian counterpart, pHSP70-polypeptide complexes can activate the immune system. It is here shown that pHSP70 purified from plant tissues transiently expressing the influenza virus nucleoprotein are able to induce both the activation of major histocompatibility complex class I-restricted polyclonal T-cell responses and antibody production in mice of different haplotypes without the need of adjuvant co-delivery. These results indicate that pHSP70 derived from plants producing recombinant antigens may be used to formulate multiepitope vaccines.
Laboratorio Biotecnologie Unità Tecnica BIORAD, ENEA C.R. Casaccia, Roma, Italy.
Giampaolo
G
Buriani
Camillo
C
Mancini
Eugenio
E
Benvenuto
Selene
S
Baschieri
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D058501:Enzyme-Linked Immunospot Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology*; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019590:Nucleocapsid Proteins; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014758:Viral Core Proteins / Q000276:immunology
D018840:HSP70 Heat-Shock Proteins; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins