Effects of low molecular weight heparin and heparin-binding epidermal growth factor on human trophoblast in first trimester.
OBJECTIVE
To evaluate the effects of low molecular weight heparin (LMWH) and heparin-binding epidermal growth factor (HB-EGF) on the biological function of human trophoblast in first trimester.


METHODS
Control experiment.


METHODS
Two academic-based reproductive centers.


METHODS
The first trimester human placentas (gestational age, 5-10 weeks) from patients who underwent electively induced abortions.


METHODS
Cultured trophoblast treated with LMWH or LMWH and HB-EGF.


METHODS
The biological function (proliferation, invasion, and differentiation) of trophoblast and the interaction between LMWH and HB-EGF on trophoblast in vitro.


RESULTS
1) At a dose of 0.25 IU/mL-2.5 IU/mL LMWH promoted trophoblast proliferation, enhanced their invasion, and increased hCG secretion. The LMWH had little effect or the opposite effect at other concentrations. These differences were statistically significant. 2) Combined use of LMWH and HB-EGF significantly promoted proliferation and invasion, but there was no difference in hCG secretion compared with solo LMWH or solo HB-EGF.


CONCLUSIONS
The LMWH exerts a cytoprotective effect by regulating trophoblast proliferation, invasion, and differentiation. The HB-EGF is an important factor in the effects of LMWH on trophoblast function.
Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
Ying
Y
Chen
Xiao-Xia
XX
Wu
Jian-ping
JP
Tan
Mei-lan
ML
Liu
Ying-lin
YL
Liu
Jian-Ping
JP
Zhang
D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D017984:Enoxaparin / Q000494:pharmacology*; D005260:Female; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D017984:Enoxaparin; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins