Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor.
A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.
Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan. g9766119@mail.nchu.edu.tw
Shu-Yi
SY
Hsu
Brandon
B
Bartling
Christina
C
Wang
Fuh-Sheng
FS
Shieu
Chung-Chiun
CC
Liu
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D004075:Diglycerides / Q000032:analysis* / Q000378:metabolism; D004209:Disposable Equipment; D057075:Enzyme Assays / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological
D004075:Diglycerides; D004800:Enzymes, Immobilized; D007495:Iridium; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase