Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A.
We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Cheng
C
Yang
Vasilica
V
Lates
Beatriz
B
Prieto-Simón
Jean-Louis
JL
Marty
Xiurong
X
Yang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D021881:DNA, Catalytic / Q000737:chemistry*; D054856:G-Quadruplexes; D057230:Limit of Detection; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D014920:Wine / Q000032:analysis*
D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A