[Expression of MMP-2 and TIMP-1 in renal tissues of patients with chronic active antibody-mediated renal graft rejection].
OBJECTIVE
To investigate the expressions of matrix metalloprotein-2 (MMP-2) and tissue inhibitor of metallopeptidase inhibitor-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (ABMR), and explore their role in the pathogenesis of ABMR.


METHODS
Immunohistochemistry and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts of 46 patients with interstitial fibrosis and tubular atrophy (IF/TA), with 15 normal renal tissue specimens as the control. The association of MMP-2 and TIMP-1 with the pathological grade of IF/TA in ABMR was analyzed.


RESULTS
The expressions of MMP-2 and TIMP-1 significantly increased in the renal tissues of the patients as compared with the normal renal tissues (P<0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased with the pathological grades of IF/TA (P<0.05). In IF/TA group, the expression of TIMP-1 was positively correlated to serum creatinine level (r=0.718, P=0.00<0.05).


CONCLUSIONS
Abnormal expressions of MMP-2 and TIMP-1 can promote the development of renal fibrosis in chronic ABMR.
Southern Medical University, Guangzhou, China. 572920410@qq.com
Bao-yao
BY
Wang
Qiang
Q
Yan
He-qun
HQ
Zou
Wei-guo
WG
Sui
Gui-mian
GM
Zuo
Gui-rong
GR
Liang
Hao
H
Luo
Shui-yong
SY
Xie
Huai-zhou
HZ
Chen
Shen-ping
SP
Xie
D000328:Adult; D000917:Antibody Formation; D015935:Complement C4b / Q000378:metabolism; D005260:Female; D005355:Fibrosis / Q000209:etiology; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007674:Kidney Diseases / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects / Q000276:immunology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
D010446:Peptide Fragments; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D015935:Complement C4b; C032261:complement C4d; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2