[Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems].
OBJECTIVE
To analyze the difference of specificity of four primers for betaproteobacterial ammonia-oxidizing bacteria (beta-AOB) 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.


METHODS
We used 16S rDNA clone libraries to describe the beta-AOB diversity.


RESULTS
CTO189f/CTO654r produced the highest frequency of beta-AOB-like sequences (67.3%). The amplification performance of primer was noticeably influenced by the biofilm samples. Hereinto, the biofilm of closed recirculation aquaculture systems of Tilapia nilotica resulted in the higher amplification performance of primers.


CONCLUSIONS
CTO189f/CTO654r exhibited the highest specific for beta-AOB 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.
Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China. peifangfang1234@163.com
Fangfang
F
Pei
Peng
P
Zhu
Hang
H
Min
Kaihong
K
Lu
Yangfang
Y
Ye
D000641:Ammonia; D017756:Aquaculture; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014871:Water Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000641:Ammonia