An alternative method for cultivation of Lawsonia intracellularis.
An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various environmental conditions for static cultivation of this bacterium and the development of diagnostic techniques.
Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA. vannu008@umn.edu
Fabio A
FA
Vannucci
Suphot
S
Wattanaphansak
Connie J
CJ
Gebhart
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D045824:Desulfovibrionaceae Infections / Q000382:microbiology / Q000662:veterinary; D005347:Fibroblasts / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000382:microbiology / Q000662:veterinary; D020639:Lawsonia Bacteria / Q000254:growth & development* / Q000302:isolation & purification; D051379:Mice; D013552:Swine