Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro.
Pro-inflammatory cytokines such as interleukin-1 beta (IL-1β) are considered to exert detrimental effects during brain trauma and in neurodegenerative disorders. Consistently, it has been demonstrated that IL-1β suppresses neurotrophin-mediated neuronal cell survival rendering neurons vulnerable to degeneration. Since neurotrophins are also well known to strongly influence axonal plasticity, we investigated here whether IL-1β has a similar negative impact on neurite growth. We analyzed neurite density and length of organotypic brain and spinal cord slice cultures under the influence of the neurotrophins NGF, BDNF, NT-3 and NT-4. In brain slices, only NT-3 significantly promoted neurite density and length. Surprisingly, a similar increase of neurite growth was induced by IL-1β. Additionally, both factors increased the number of brain slices displaying maximal neurite growth. Furthermore, the co-administration of IL-1β and NT-3 significantly increased the number of brain slices displaying maximal neurite growth compared to single treatments. These data indicate that these two factors synergistically stimulate two distinct aspects of neurite outgrowth, namely neurite density and neurite length from acute organotypic brain slices.
Dept. of Functional Morphology & BIOMED Institute, Hasselt University, Belgium.
Francesco
F
Boato
Daniel
D
Hechler
Karen
K
Rosenberger
Doreen
D
Lüdecke
Eva M
EM
Peters
Robert
R
Nitsch
Sven
S
Hendrix
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020932:Nerve Growth Factor / Q000378:metabolism / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D009473:Neuronal Plasticity / Q000502:physiology; D020933:Neurotrophin 3 / Q000378:metabolism* / Q000494:pharmacology; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism
D053583:Interleukin-1beta; D020933:Neurotrophin 3; D020932:Nerve Growth Factor