Antimicrobial activity of Amazonian oils against Paenibacillus species.
The Gram-positive, spore-forming bacterium Paenibacillus larvae is the primary bacterial pathogen of honeybee brood and the causative agent of American foulbrood disease (AFB). One of the feasible alternative treatments being used for their control of this disease is essential oils. In this study in vitro antimicrobial activity of Andiroba and Copaíba essential oils against Paenibacillus species, including P. larvae was evaluated. Minimal inhibitory concentration (MIC) in Mueller-Hinton broth by the microdilution method was assessed. Andiroba registered MIC values of 1.56-25%, while the MICs values obtained for Copaíba oil were of 1.56-12.5%. In order to determine the time-response effect of essential oils on P. larvae, this microorganism was exposed to the oils for up to 48 h. After 24 h treatment with Andiroba oil and after 48 h treatment with Copaíba oil no viable cells of P. larvae ATCC 9545 were observed. The possible toxic effect of essential oils were assessed by the spraying application method of the same concentrations of MICs. Bee mortality was evident only in treatment with Andiroba oil and the Copaíba oil shows no toxic effects after 10 days of observation. Taking together ours results showed for the first time that these oils presented a high activity against Paenibacillus species showing that Copaíba oil may be a candidate for the treatment or prevention of AFB.
Laboratório de Microbiologia, Ciências da Saúde, Centro Universitário Franciscano, UNIFRA, Santa Maria, Rio Grande do Sul, Brazil. robertochrist@gmail.com
Roberto Christ Vianna
RC
Santos
Camilla Filippi
CF
dos Santos Alves
Taiane
T
Schneider
Leonardo Quintana Soares
LQ
Lopes
Carlos
C
Aurich
Janice Luehring
JL
Giongo
Adriano
A
Brandelli
Rodrigo
R
de Almeida Vaucher
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001516:Bees / Q000382:microbiology*; D007887:Fabaceae / Q000737:chemistry*; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D056507:Paenibacillus / Q000187:drug effects*
D000890:Anti-Infective Agents; D009822:Oils, Volatile