Anti-asthmatic agents alleviate pulmonary edema by upregulating AQP1 and AQP5 expression in the lungs of mice with OVA-induced asthma.
Ovalbumin (OVA)-induced asthma in mouse lungs causes changes in the mRNA and protein levels of aquaporins (AQPs). AQP expression was examined in the presence of various anti-asthmatic agents, including dexamethasone, ambroxol, and terbutaline. The influence of these agents on OVA-induced airway inflammation was also evaluated. The mRNA expression levels of AQP1, 4, and 5 were significantly reduced and that of AQP3 was significantly increased 24h after the last OVA exposure. The protein levels of AQP1, 3, and 5 mirrored the mRNA expression profiles, but AQP4 did not exhibit any changes. Only the mRNA and protein expression levels of AQP1 and AQP5 were significantly increased by these three anti-asthmatic agents. Dexamethasone and ambroxol improved the eosinophil infiltration, mucus secretion, and pulmonary edema caused by OVA, but terbutaline only alleviated pulmonary edema. These results indicate that AQP1 and AQP5 are closely related to pulmonary edema but not to eosinophil infiltration or mucus secretion during asthma. Anti-asthmatic agents could alleviate pulmonary edema through upregulating the expression of AQP1 and AQP5 in mouse lungs that have OVA-induced asthma.
Department of Pulmonary Medicine, Research Institute of Respiratory Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Chunling
C
Dong
Guifang
G
Wang
Bo
B
Li
Kui
K
Xiao
Zhongsen
Z
Ma
Hua
H
Huang
Xiangdong
X
Wang
Chunxue
C
Bai
D000551:Ambroxol / Q000627:therapeutic use; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D051398:Aquaporin 1 / Q000187:drug effects / Q000378:metabolism*; D051402:Aquaporin 5 / Q000187:drug effects / Q000235:genetics; D001249:Asthma / Q000150:complications* / Q000188:drug therapy; D003907:Dexamethasone / Q000627:therapeutic use; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051379:Mice; D011654:Pulmonary Edema / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000187:drug effects*; D013726:Terbutaline / Q000627:therapeutic use; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D018927:Anti-Asthmatic Agents; C496021:Aqp1 protein, mouse; C496038:Aqp5 protein, mouse; D051402:Aquaporin 5; D012333:RNA, Messenger; D051398:Aquaporin 1; D000551:Ambroxol; D003907:Dexamethasone; D013726:Terbutaline