Estimation of dermatological application of creams with St. John's Wort oil extracts.
Oleum Hyperici, the oil extract of St. John's Wort (SJW), is one of the oldest folk remedies, traditionally used in the topical treatment of wounds, bruises, ulcers, cuts, burns, hemorrhoids and also as an antiseptic. Considering the advantageous characteristics of emulsion applications, in the present study we have formulated three O/W creams containing 15% (w/v) of SJW oil extract as an active ingredient. The aim was to estimate dermatological application of the prepared creams for the abovementioned indications. The extracts were prepared according to the prescriptions from traditional medicine, however with different vegetable oils used as an extractant, namely: Olive, palm and sunflower oil. The investigated O/W creams demonstrated significant antiinflammatory effects in an in vivo double-blind randomized study, using a sodium lauryl sulphate test. Both skin parameters assessed in the study (electrical capacitance and erythema index), were restored to the baseline value after a seven-day treatment with the tested creams. Almost all investigated SJW oil extracts and corresponding creams displayed the same antimicrobial activity against the most of the investigated microorganisms with obtained minimal inhibitory concentrations values of 1,280 µg/mL, 2,560 µg/mL or >2,560 µg/mL.
Department of Pharmaceutical Research and Development, Institute for Medicinal Plant Research Dr Josif Pančić, Tadeuša Košćuška 1, 11 000 Belgrade, Serbia. iarsic@mocbilja.rs
Ivana
I
Arsić
Ana
A
Zugić
Vanja
V
Tadić
Marija
M
Tasić-Kostov
Dušan
D
Mišić
Marija
M
Primorac
Dušanka
D
Runjaić-Antić
D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical; D004339:Drug Compounding; D004355:Drug Stability; D025802:Electric Capacitance; D004890:Erythema; D006801:Humans; D020902:Hypericum / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry*; D012867:Skin / Q000187:drug effects
D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D010938:Plant Oils