Correlation between leukocyte membrane lipid peroxidation and expression of Cu/Zn-superoxide dismutase mRNA in hemodialysis patients.
BACKGROUND
Previously, we reported an increase in expressions of mRNA of Cu/Zn-superoxide dismutase (SOD) in leukocytes of hemodialysis (HD) patients, and speculated that the increase is associated with oxidative stress on the leukocyte membrane due to the HD process.


METHODS
Expressions of Cu/Zn-SOD mRNA in leukocytes, contents of plasma SOD, and malondialdehyde (MDA) in leukocyte and erythrocyte membranes, respectively, were examined in 25 HD patients and 14 healthy volunteers. These were also determined after using a vitamin E-coated dialyzer (VE dialyzer) for 4 weeks.


RESULTS
All values were significantly higher in HD patients. A significant correlation was found between leukocyte Cu/Zn-SOD mRNA expression and membrane MDA. After using the VE dialyzer, all values were significantly lowered, showing a significant correlation between changing rate of leukocyte Cu/Zn-SOD mRNA expression and membrane MDA.


CONCLUSIONS
In HD patients, oxidative stress is generated on the leukocyte membrane, and the level of Cu/Zn-SOD mRNA in leukocytes can be a useful oxidative stress marker.
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan. washio@med.showa-u.ac.jp
Kazunori
K
Washio
Masahiro
M
Inagaki
Mayumi
M
Tsuji
Yuri
Y
Morio
Hiromichi
H
Gotoh
Yoshikazu
Y
Gotoh
Katsuji
K
Oguchi
D000368:Aged; D005260:Female; D015870:Gene Expression; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics*; D006435:Renal Dialysis; D013482:Superoxide Dismutase / Q000235:genetics*
D008563:Membrane Lipids; D012333:RNA, Messenger; D008315:Malondialdehyde; D013482:Superoxide Dismutase