[GC-MS analysis and analgesic activity of essential oil from fresh rhizoma of Cyperus rotundus].
OBJECTIVE
To observe the analgesic effect and analysis the chemical components of essential oil (EOC) from fresh rhizome of Cyperus rotundus.


METHODS
Hydrodistillation method was used to extract EOC; Acetic acid induced mice stretching model was applied to evaluate the analgesic effect of EOC. Gas chromatography coupled with mass spectrometry (GC-MS) were applied for the analysis of EOC.


RESULTS
The yield of EOC was 0.4%. The stretching times of mice induced by acetic acid were significantly reduced by EOC at dosage of 0.1 g/kg. 54 ingredients, 85.1% of which were EOC were identified by GC-MS analysis; The major compounds were cyperene (41.03%), beta-caryophyllene oxide (5.32%), alpha-selinene (4.37%), alpha-copaene (4.36%), naphthalene, 6-isoproenyl-4,8a-dimethyl-1, 2, 3, 5, 6, 7, 8, 8a-octahydro-(3.80%) and alpha-cyperone (3.11%).


CONCLUSIONS
The essential oil from fresh rhizome of Cyperus rotundus shows structure diversity and good analgesic effect.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. yoona_cy@sjtu.edu.cn
Yun
Y
Chen
Yun-Yu
YY
Zhao
Xiao-Yi
XY
Wang
Jin-Tao
JT
Liu
Li-Qun
LQ
Huang
Chong-Sheng
CS
Peng
D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D029786:Cyperus / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010146:Pain / Q000188:drug therapy / Q000503:physiopathology; D017288:Pain Threshold / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D000081223:Polycyclic Sesquiterpenes; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000032:analysis*; D012997:Solvents
D000700:Analgesics; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D012997:Solvents; C539285:selinene; D019342:Acetic Acid; C515179:caryophyllene oxide