Array-based yeast two-hybrid screens: a practical guide.
Yeast two-hybrid screens are carried out as random library screens or matrix-based screens. The latter have the advantage of being better controlled and thus typically give clearer results. In this chapter, we provide detailed protocols for matrix-based Y2H screens and give some helpful instructions how to plan a large-scale interaction screen. We also discuss strategies to identify or avoid false negatives and false positives.
Karlsruhe Institute of Technology, Karlsruhe, Germany.
Roman
R
Häuser
Thorsten
T
Stellberger
Seesandra V
SV
Rajagopala
Peter
P
Uetz
D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D059765:Homologous Recombination; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D040081:Protein Array Analysis / Q000379:methods*; D013997:Time Factors; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques / Q000295:instrumentation*; D015003:Yeasts / Q000235:genetics