Glomerella truncata: another Glomerella species with an atypical mating system.
In the genus Glomerella all species studied to date do not fit the usual mating system of heterothallic ascomycetes. This study investigated the mating system of G. truncata (anamorph Colletotrichum truncatum), a pathogen responsible for lentil anthracnose. Twenty-two field isolates from the Canadian prairies were crossed in all possible combinations, including selfings. All isolates also were screened for the presence of the MAT1-1 and MAT1-2 idiomorphs by targeting small conserved areas of the MAT genes (the alpha domain and the high mobility group HMG box) with degenerate primers, and a pair of G. truncata-specific HMG primers (CT21HMG) were designed. The results of the classical mating study suggested that G. truncata is heterothallic. Isolates fell into two incompatibility groups, which is consistent with a bipolar mating system but different from what has been described in other Glomerella species. Molecular screening showed that the HMG box used as a marker for the MAT1-2 idiomorph was present in both partners of fertile crosses in G. truncata, unlike in the typical ascomycete system, but as previously described for two other Glomerella species. G. truncata therefore appears to share unusual mating system characteristics with the other Glomerella species studied to date.
Department of Plant Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Jennifer
J
Menat
Adrian L
AL
Cabral
Perumal
P
Vijayan
Yangdou
Y
Wei
Sabine
S
Banniza
D000595:Amino Acid Sequence; D001483:Base Sequence; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D049770:Genes, Mating Type, Fungal / Q000235:genetics*; D024742:HMG-Box Domains / Q000235:genetics*; D027806:Lens Plant / Q000382:microbiology*; D008969:Molecular Sequence Data; D020081:Phyllachorales / Q000145:classification / Q000235:genetics* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers