Genes encoding OXA-134-like enzymes are found in Acinetobacter lwoffii and A. schindleri and can be used for identification.
bla(OXA-134) genes and variants were sought in 21 species of Acinetobacter and found in A. lwoffii, genomic species 9 (regarded as synonyms), and A. schindleri. Sequencing revealed a 9-bp deletion in the gene in the type strain of genomic species 9 (ATCC 9957) relative to the gene in the type strain of A. lwoffii (ATCC 15309). Primers based on the gene without the deletion gave specific amplification of 29 of 30 clinical isolates of A. lwoffii/genomic species 9.
Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, London, United Kingdom. jane.turton@hpa.org.uk
Jane F
JF
Turton
Rhiannon
R
Hyde
Kate
K
Martin
Jayesh
J
Shah
D000150:Acinetobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D001618:beta-Lactamases / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases