Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device.
To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that utilizes these two dispersal proteins along with a population-driven quorum-sensing switch. With this synthetic circuit, in a novel microfluidic device, we form an initial colonizer biofilm, introduce a second cell type (dispersers) into this existing biofilm, form a robust dual-species biofilm and displace the initial colonizer cells in the biofilm with an extracellular signal from the disperser cells. We also remove the disperser biofilm with a chemically induced switch, and the consortial population could tune. Therefore, for the first time, cells have been engineered that are able to displace an existing biofilm and then be removed on command allowing one to control consortial biofilm formation for various applications.
Department of Chemical Engineering, Texas A & M University, College Station, Texas 77843-3122, USA.
Seok Hoon
SH
Hong
Manjunath
M
Hegde
Jeongyun
J
Kim
Xiaoxue
X
Wang
Arul
A
Jayaraman
Thomas K
TK
Wood
D000188:Actinobacillus / Q000378:metabolism; D001089:Arabinose / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D018441:Biofilms; D018533:Biomass; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics; D010461:Peptones / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015202:Protein Engineering / Q000379:methods; D053038:Quorum Sensing; D013997:Time Factors
C559839:BdcA protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D010461:Peptones; C079209:hha protein, E coli; C001830:tryptones; D001089:Arabinose