Increased prevalence of torque teno viruses in porcine respiratory disease complex affected pigs.
The role of swine torque teno sus viruses (TTSuVs) as co-factors in disease syndromes involving porcine circovirus strain 2 (PCV2) and porcine reproductive and respiratory disease syndrome virus (PRRSV) has been a debatable subject. In this study, the prevalence of TTSuVs in Iowa, the leading pork producing state in the U.S., was estimated by a duplex PCR. The PCR is capable of simultaneously detecting both teno sus viruses 1 and 2 (TTSuV1 and 2). Based on an analysis of 300 random samples representing six major geographical regions of the state, the overall prevalence rates for TTSuV1 and 2 were 47.34% and 24.67% respectively while the combined prevalence rate was 52.33%. The epidemiological association of TTSuV1 and 2 with the common etiological agents of the porcine respiratory disease complex (PRDC) namely porcine PRRSV, PCV2, Mycoplasma hyopneumoniae and swine influenza virus (SIV) was estimated in lung tissue derived from 45 pigs showing clinical signs of PRDC. Notably, 86.67% of the PRDC-suspect samples were positive for TTSuV1 in comparison to the baseline population prevalence rate of 47.34%. However, the prevalence of TTSuV2 (26.67%) was not significantly different. TTSuV1 was detected in 80.00%, 81.81%, 75.00% and 77.78% of the PRRSV, SIV, M. hyopneumoniae and PCV2 positive PRDC-suspect samples respectively. Our results indicate that TTSuV1 is strongly associated with clinical PRDC and support the hypothesis that TTSuVs might function as co-factors in PRDC. Further studies to define their possible role in the pathogenesis of swine respiratory diseases are warranted.
Department of Veterinary Diagnostic and Production Animal Medicine, Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA 50011, USA.
Lavanya
L
Rammohan
Lin
L
Xue
Chong
C
Wang
Wayne
W
Chittick
Shanthi
S
Ganesan
Sheela
S
Ramamoorthy
D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology* / Q000821:virology; D017925:Circovirus / Q000472:pathogenicity; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype / Q000472:pathogenicity; D007484:Iowa / Q000453:epidemiology; D008168:Lung / Q000473:pathology / Q000821:virology; D045705:Mycoplasma hyopneumoniae / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity; D015995:Prevalence; D012140:Respiratory Tract Diseases / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D013552:Swine / Q000821:virology; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*