Understanding the α-crystallin cell membrane conjunction.
OBJECTIVE
It is well established that levels of soluble α-crystallin in the lens cytoplasm fall steadily with age, accompanied by a corresponding increase in the amount of membrane-bound α-crystallin. Less well understood, is the mechanism driving this age-dependent membrane association. The aim of this study was to investigate the role of the membrane and its associated proteins and peptides in the binding of α-crystallin.


METHODS
Fiber cell membranes from human and bovine lenses were separated from soluble proteins by centrifugation. Membranes were stripped of associated proteins with successive aqueous, urea, and alkaline solutions. Protein constituents of the respective membrane isolates were examined by SDS-PAGE and western immunoblotting. Recombinant αA- and αB-crystallins were fluorescently-labeled with Alexa350® dye and incubated with the membrane isolates and the binding capacity of membrane for α-crystallin was determined.


RESULTS
The binding capacity of human membranes was consistently higher than that of bovine membranes. Urea- and alkali-treated membranes from the nucleus had similar binding capacities for αA-crystallin, which were significantly higher than both cortical membrane extracts. αB-Crystallin also had a higher affinity for nuclear membrane. However, urea-treated nuclear membrane had three times the binding capacity for αB-crystallin as compared to the alkali-treated nuclear membrane. Modulation of the membrane-crystallin interaction was achieved by the inclusion of an NH₂-terminal peptide of αB-crystallin in the assays, which significantly increased the binding. Remarkably, following extraction with alkali, full length αA- and αB-crystallins were found to remain associated with both bovine and human lens membranes.


CONCLUSIONS
Fiber cell membrane isolated from the lens has an inherent capacity to bind α-crystallin. For αB-crystallin, this binding was found to be proportional to the level of extrinsic membrane proteins in cells isolated from the lens nucleus, indicating these proteins may play a role in the recruitment of αB-crystallin. No such relationship was evident for αA-crystallin in the nucleus, or for cortical membrane binding. Intrinsic lens peptides, which increase in abundance with age, may also function to modulate the interaction between soluble α-crystallin and the membrane. In addition, the tight association between α-crystallin and the lens membrane suggests that the protein may be an intrinsic component of the membrane structure.
School of Biological Sciences, University of Wollongong, Wollongong, NSW, Australia.
Shih-Ping
SP
Su
Jason D
JD
McArthur
Michael G
MG
Friedrich
Roger J W
RJ
Truscott
J Andrew
JA
Aquilina
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D000468:Alkalies; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D006801:Humans; D007904:Lens Cortex, Crystalline / Q000033:anatomy & histology / Q000378:metabolism*; D007907:Lens Nucleus, Crystalline / Q000033:anatomy & histology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D011485:Protein Binding; D014020:Tissue Extracts / Q000737:chemistry; D014508:Urea; D038202:alpha-Crystallin A Chain / Q000302:isolation & purification / Q000378:metabolism*; D038203:alpha-Crystallin B Chain / Q000302:isolation & purification / Q000378:metabolism*
D000468:Alkalies; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D014020:Tissue Extracts; D038202:alpha-Crystallin A Chain; D038203:alpha-Crystallin B Chain; D014508:Urea