The application of genome-wide RNAi screens in exploring varieties of signaling transduction pathways.
Cardiovascular development is a precisely coordinated process at multilevels. It involves cross-talking among numerous signaling transduction pathways to ensure proper cell polarity, migration, proliferation, differentiation, and programmed death. Here, genome-wide RNA interference screens in Drosophila cells are introduced as novel approaches to discover potential regulators, with special emphases on (1) cell growth and viability, and (2) receptor tyrosine kinase and extracellular-signal-regulated kinase signaling pathway.
Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Healthy Science Center, Temple, TX, USA. shenyuan.zhang@medicine.tamhsc.edu
Shenyuan
S
Zhang
Hongying
H
Zheng
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D023281:Genomics / Q000379:methods* / Q000592:standards; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012015:Reference Standards; D015398:Signal Transduction / Q000235:genetics*
D012330:RNA, Double-Stranded; D048049:Extracellular Signal-Regulated MAP Kinases