GC-MS and spectrophotometric analysis of biodegradation of new disazo dye by Trametes versicolor.
In this study; sub-tropical white rot fungi, Trametes versicolor was investigated for its ability to degrade 4-(3'-methyl-4'-(4"-nitrophenyl)azo- 1'H-pyrazol-5'-ylazo)-3-methyl- H-pyrazol-5-on in the mediums containing glucose and different concentrations of degrade dye in batch systems. This dye was synthetized at Pamukkale Universtiy of Organic Chemistry research laboratory. Samples were collected on 10 days, and was detected by Shimadzu UV-1600A spectrophotometry. Decolorization study showed that this disazo dye was removed by more than 70% in 10 days. Laccase enzyme activity was detected in samples and then last sample was analyzed by GC-MS. Metabolites weren't showed in GC-MS result. It was concluded that T. versicolor could achieve the biodegradation of this new disazo dye.
The University of Pamukkale, Faculty of Science & Arts, Department of Chemistry, P.O. 286, 20017 Denizli, Turkey. hardag@pamukkale.edu.tr
H Ardag
HA
Akdogan
A
A
Demircali
C
C
Aydemir
N
N
Pazarlioglu
F
F
Karci
D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D042845:Laccase / Q000737:chemistry / Q000378:metabolism; D055454:Trametes / Q000737:chemistry / Q000201:enzymology*
C569871:4-(3'-methyl-4'-(4''-nitrophenyl)azo-1'H-pyrazol-5'-ylazo)-3-methyl-H-pyrazol-5-one; D001391:Azo Compounds; D004396:Coloring Agents; D042845:Laccase