Magnetic stent hyperthermia for esophageal cancer: an in vitro investigation in the ECA-109 cell line.
Magnetic stent hyperthermia (MSH) is a novel approach for targeted thermotherapy             for esophageal cancer, which is based on the mechanism that inductive heat can             be generated by the esophageal stent upon exposure under an alternative magnetic             field (AMF). A positive effect of MSH on esophageal cancer has been demonstrated,             however, there is no study on the in vitro effects of heating treatment or of             the effects of AMF exposure on human esophageal cancer cells. This study aimed             to investigate the effect of MSH and of AMF exposure in esophageal cancer cells.             Inductive heating characteristics of esophageal stents were assessed by exposing             the stents under AMF. A rather rapid temperature rise of the Ni-Ti stent when             subjected to AMF exposure was observed and the desired hyperthermic temperature             could be controlled by adjusting the field parameter of the AMF. Human esophageal             squamous carcinoma (ESCC) ECA-109 cells were divided into four groups: the control             group, the water-bath heating group, the MSH group and the AMF exposure group.             Hyperthermic temperatures were 43, 48 and 53˚C and the treatment time was in the             range of 5-30 min. The MTT assay, apoptotic analysis and TUNEL staining were applied             in the current investigation. Exposure of ECA-109 cells under AMF with a field             intensity of 50 to 110 kA/m had negligible effect on cell viability, cell necrosis             and apoptosis. Hyperthermia had a remarkable inhibitory effect on the cell viability             and the effect was dependent on the thermal dose (temperature and time). The optimal             thermal dose of MSH for ECA-109 cells was 48˚C for 20-30 min. The study also elucidated             that there was a difference in the effects on cell necrosis and apoptosis between             the heating mode of water bath and MSH. The data suggest that MSH may have clinical             significance for esophageal cancer treatment.
Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, PR China.
Jia-Yi
JY
Liu
Ling-Yun
LY
Zhao
Yu-Ying
YY
Wang
Dan-Ye
DY
Li
Dan
D
Tao
Li-Ya
LY
Li
Jin-Tian
JT
Tang
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004938:Esophageal Neoplasms / Q000473:pathology / Q000628:therapy*; D006361:Heating / Q000379:methods; D006801:Humans; D006979:Hyperthermia, Induced / Q000295:instrumentation / Q000379:methods*; D020287:In Situ Nick-End Labeling / Q000379:methods; D060526:Magnetic Fields; D008280:Magnetics / Q000379:methods; D009336:Necrosis; D009532:Nickel / Q000627:therapeutic use; D015607:Stents; D013696:Temperature; D014025:Titanium / Q000627:therapeutic use
C040654:titanium nickelide; D009532:Nickel; D014025:Titanium