E-cadherin and caveolin-1 alterations in the heart of rats having undergone             chlorpromazine-induced toxicity.
Heart damage induced by chlorpromazine (CPZ) toxicity is associated with changes in the expression of various enzymes and proteins. This study aimed to investigate CPZ‑induced alterations in cardiac E-cadherin and caveolin-1 (cav-1) after CPZ administration. Male Wistar rats were randomly divided into two groups: a control group and a CPZ group. The CPZ group was administered CPZ intraperitoneally at a single dose of 10 mg/kg for 21 days; the controls were given the same amount of saline via the same route. On Day 22, the rats were anesthetized, and a thoracotomy was performed in all animals. Immunohistochemical analysis was performed to evaluate protein expression of E-cadherin and cav-1. Sections were analyzed by digital image analysis. Results of the present study revealed that cardiac protein expression of E-cadherin and cav-1 was altered after CPZ-induced toxicity in the rat. The expression of E-cadherin was significantly reduced, while expression of cav-1 was significantly increased after CPZ treatment, as supported by integrated optical density analysis, compared with the control (P<0.05). The current findings indicate that such changes in the expression of E-cadherin and cav-1 may be reflected in abnormal cardiac function, and these proteins may be useful in revealing the mechanisms underlying CPZ-induced toxicity and may also provide additional insight for further research.
Department of Pathology, School of Basic Medical Sciences, Dali University, Dali, Yunnan 671000, PR China. hqy0726@126.com
Quan-Yong
QY
Huang
Xue-Fang
XF
Li
Shui-Ping
SP
Liu
D000818:Animals; D000932:Antiemetics / Q000633:toxicity*; D015820:Cadherins / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D002746:Chlorpromazine / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D000932:Antiemetics; D015820:Cadherins; D051242:Caveolin 1; D002746:Chlorpromazine