Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryziaslatipes) exposed to 17α-ethinylestradiol.
The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L(-1) of 17α-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzoate (EB) for 3 weeks (experiment I), and a measured concentration of 20 ng L(-1) EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L(-1) EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L(-1) EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka.
The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8513, Japan.
Ikumi
I
Hirakawa
Shinichi
S
Miyagawa
Yoshinao
Y
Katsu
Yoshihiro
Y
Kagami
Norihisa
N
Tatarazako
Tohru
T
Kobayashi
Teruhiko
T
Kusano
Takeshi
T
Mizutani
Yukiko
Y
Ogino
Takashi
T
Takeuchi
Yasuhiko
Y
Ohta
Taisen
T
Iguchi
D000818:Animals; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000378:metabolism / Q000633:toxicity*; D029941:Fish Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism; D008297:Male; D009865:Oocytes; D010063:Ovum; D013737:Testis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D052244:Endocrine Disruptors; D029941:Fish Proteins; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol