First report of the multidrug resistance gene cfr in Enterococcus faecalis of animal origin.
The multiresistance gene cfr was identified for the first time in an Enterococcus faecalis isolate of animal origin. The 32,388-bp plasmid pEF-01, which carried the cfr gene, was sequenced completely. Three copies of the insertion sequence IS1216 were identified in pEF-01, and the detection of a cfr- and IS1216-containing amplicon by inverse PCR suggests that IS1216 may play a role in the dissemination of cfr by a recombination process.
Key Laboratory of Development and Evaluation of Chemical and Herbal Drugs for Animal Use, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Yang
Y
Liu
Yang
Y
Wang
Congming
C
Wu
Zhangqi
Z
Shen
Stefan
S
Schwarz
Xiang-Dang
XD
Du
Lei
L
Dai
Wanjiang
W
Zhang
Qijing
Q
Zhang
Jianzhong
J
Shen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002701:Chloramphenicol / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018628:Gene Dosage; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D008780:Methyltransferases / Q000235:genetics*; D016254:Mutagenesis, Insertional; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D002701:Chloramphenicol; C035534:florfenicol; D008780:Methyltransferases; D013839:Thiamphenicol