TNF-α expression in Schwann cells is induced by LPS and NF-κB-dependent pathways.
Lipopolysaccharide (LPS) is recognized by Toll-like receptor 4 and activates mitogen-activated protein kinase, which leads to the induction of proinflammatory cytokine gene expression. In vivo, Schwann cells (SCs) at the site of injury may also produce tumor necrosis factor-α (TNF-α). However, the precise mechanism that regulates TNF-α synthesis is still not clear. The nuclear transcription factor-κB (NF-κB) is an important transcription factor which is involved in the regulation of host immune responses. In the present study, we found that LPS possessed a comparable specific activity for activation of NF-κB-dependent gene expression in SCs. We also observed IκB-α/IκB-β degradation and the nuclear translocation of P65 due to LPS treatments. LPS-elicited TNF-α production in SCs was also drastically suppressed by SN50 (NF-κB inhibitor).
Department of Pathogen Biology, Medical College, Nantong University, 19 Qixiu Road, Nantong, 226001 Jiangsu, China.
Yongwei
Y
Qin
Minhui
M
Hua
Yinong
Y
Duan
Yongjing
Y
Gao
Xiaoyi
X
Shao
Haibo
H
Wang
Tao
T
Tao
Aiguo
A
Shen
Chun
C
Cheng
D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016328:NF-kappa B / Q000819:agonists / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000819:agonists / Q000096:biosynthesis*; D015854:Up-Regulation / Q000502:physiology
D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha