Effect of spinal and general anesthesia on serum concentration of pro-inflammatory and anti-inflammatory cytokines.
BACKGROUND
Surgery induces release of neuroendocrine hormones, cytokines and acute phase proteins. The aim of this study was to assess the effect of spinal and general anesthesia on serum concentration of pro-inflammatory and anti-inflammatory cytokines, and cytokines which are secreted by Th1 helper lymphocytes.


METHODS
30 patients with American Society of Anesthesiologists status I and II who were scheduled for TURP (Transurethral Resection of the Prostata) were anesthetized in regional (spinal) or general anesthesia. Peripheral venous blood samples were collected 2 h before surgery on the first, third and fifth postoperative days. We measured pro-inflammatory cytokines, anti-inflammatory cytokines and cytokines which are secreted by Th1 helper lymphocytes in order to establish differences in patients before and after surgery.


RESULTS
Statistically significant differences were found in serum levels of interleukin-2 (IL-2) between general and spinal anesthesia (p=0.043). The concentration of IL-2 was continuously elevated in general anesthesia, but not in spinal anesthesia. It is important to note that the preoperative serum IL-2 concentration in general anesthesia group was significantly higher in comparison to spinal anesthesia group (p=0.028). There was also statistically significant increase of interleukin-6 (IL-6) in spinal (p=0.043) and general anesthesia (p=0.03) in comparison to preoperative value.


CONCLUSIONS
Surgery-related postoperative release of the pro-inflammatory cytokine IL-6 was increased in patients after spinal and general anesthesia. In our study, increased levels of the typical Th1 cytokine IL-2 were found in patients anesthetized by general anesthesia compared to spinal anesthesia. Serum concentrations of other pro-inflammatory cytokines, anti-inflammatory cytokines and cytokines which are secreted by Th1 helper lymphocytes showed no statistical difference before and after surgery under general and spinal anesthesia.
Department of Anaesthesiology, Reanimatology and Intensive Medicine, University Hospital Center - Zagreb, Croatia.
Marijana
M
Žura
Ana
A
Kozmar
Katarina
K
Šakić
Branko
B
Malenica
Zlatko
Z
Hrgovic
D000368:Aged; D000768:Anesthesia, General; D000775:Anesthesia, Spinal; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood* / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000276:immunology / Q000601:surgery; D058408:Th1-Th2 Balance; D020728:Transurethral Resection of Prostate
D007376:Interleukin-2; D015850:Interleukin-6