Fast mapping of biomolecular interfaces by Random Spin Labeling (RSL).
Random spin labeling (RSL) is a method for rapid mapping of biomolecular interaction surfaces using an interaction partner with SL and an interaction partner enriched in (13)C or (15)N nuclei for paramagnetic relaxation enhanced NMR-based detection. The SL reaction is conducted in a manner resulting in a heterogeneous reaction product consisting of different populations of the protein carrying a varying number of spin labels at different positions. Preparation of the paramagnetic probe is complete within a few hours and hence much faster than site selective SL. RSL is applicable to tightly interacting systems but shows its particular strength when applied to systems involving weak or transient contacts.
Lehrstuhl Biopolymere, Universitat Bayreuth, Universitatsstrasse 30, 95440 Bayreuth, Germany.
Maximilian J
MJ
Hartl
Björn M
BM
Burmann
Stefan J
SJ
Prasch
Clemens
C
Schwarzinger
Kristian
K
Schweimer
Birgitta M
BM
Wöhrl
Paul
P
Rösch
Stephan
S
Schwarzinger
D004578:Electron Spin Resonance Spectroscopy; D009682:Magnetic Resonance Spectroscopy; D019906:Nuclear Magnetic Resonance, Biomolecular; D013113:Spin Labels
D013113:Spin Labels