Transferable multidrug resistance plasmid carrying cfr associated with tet(L), ant(4')-Ia, and dfrK genes from a clinical methicillin-resistant Staphylococcus aureus ST125 strain.
A multidrug resistance (MDR) conjugative plasmid of ca. 50 kb (designated pERGB) was detected in a linezolid and methicillin-resistant Staphylococcus aureus strain with sequence type 125 (ST125-MRSA-IVc). This strain was detected in two patients with chronic obstructive pulmonary disease, previously treated with multiple antimicrobials, including linezolid. pERGB was transferable by conjugation and carried the resistance genes cfr (oxazolidinones, phenicols, lincosamides, pleuromutilins, and streptogramin A), ant(4')-Ia (tobramycin), tet(L) (tetracycline), and dfrK (trimethoprim). A novel genetic structure, linking all of these resistance genes for the first time, was elucidated through sequencing of a 15,259-bp fragment from pERGB. Active surveillance to prevent the dissemination of such highly concerning MDR transferable elements is needed.
Servicio de Microbiología, Hospital Universitari Son Espases, Palma de Mallorca, Spain. enrique.ruiz@ssib.es
Enrique Ruiz de
ER
Gopegui
Carlos
C
Juan
Laura
L
Zamorano
José L
JL
Pérez
Antonio
A
Oliver
D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D000069349:Linezolid; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D023303:Oxazolidinones / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000382:microbiology; D013030:Spain
D000081:Acetamides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C539943:CFR protein, Staphylococcus aureus; D004269:DNA, Bacterial; D023303:Oxazolidinones; C539941:TetL protein, Staphyloccus aureus; C428104:Ant(4')-Ia protein, Staphylococcus aureus; D009713:Nucleotidyltransferases; D000069349:Linezolid