Monitoring of environmental arsenic by cultures of the photosynthetic bacterial sensor illuminated with a near-infrared light emitting diode array.
Recombinant Rhodopseudomonas palustris, harboring the carotenoid-metabolizing gene crtI (CrtIBS), and whose color changes from greenish yellow to red in response to inorganic As(III), was cultured in transparent microplate wells illuminated with a light emitting diode (LED) array. The cells were seen to grow better under near-infrared light, when compared with cells illuminated with blue or green LEDs. The absorbance ratio of 525 to 425 nm after cultivation for 24 h, which reflects red carotenoid accumulation, increased with an increase in As(III) concentrations. The detection limit of cultures illuminated with near-infrared LED was 5 microgram/l, which was equivalent to that of cultures in test tubes illuminated with an incandescent lamp. A near-infrared LED array, in combination with a microplate, enabled the simultaneous handling of multiple cultures, including CrtIBS and a control strain, for normalization by the illumination of those with equal photon flux densities. Thus, the introduction of a near-infrared LED array to the assay is advantageous for the monitoring of arsenic in natural water samples that may contain a number of unknown factors and, therefore, need normalization of the reporter event.
Faculty of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya 321-8505, Japan. i-maeda@cc.utsunomiya-u.ac.jp
Isamu
I
Maeda
Hirokazu
H
Sakurai
Kazuyuki
K
Yoshida
Mohammad
M
Siddiki
Tokuo
T
Shimizu
Motohiro
M
Fukami
Shunsaku
S
Ueda
D001151:Arsenic / Q000032:analysis* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002338:Carotenoids / Q000378:metabolism; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D008027:Light; D010788:Photosynthesis; D012241:Rhodopseudomonas / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
D001426:Bacterial Proteins; D014874:Water Pollutants, Chemical; D002338:Carotenoids; D001151:Arsenic