[Study on the activated fraction with proliferation and apoptosis effect on T lymphocyte of Celastrus aculeatus].
OBJECTIVE
To screen the activated fraction from Celastrus aculeatus and study its effect on T lymphocyte apoptosis.


METHODS
Different polarity fractions were isolated from Celastrus aculeatus extract. Flow Cytometry method was used to detect apoptosis ratio of the active fractions.


RESULTS
Different fractions extract from Celastrus aculeatus and GSF-A could significantly inhibit T lymphocyte proliferation and induce T lymphocyte apoptosis.


CONCLUSIONS
The activated fractions isolated from Celastrus aculeatus have anti-inflammatory effect. Its mechanism may be related to the apoptosis effect on T lymphocyte.
School of Chinese Medicine, Southern Medical University, Guangzhou 510515, China. cph1216@163.com
Pei-Hong
PH
Chen
Zong-Bao
ZB
Ding
Yang
Y
Xie
Li
L
Tong
D000085:Acetates / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D031247:Celastrus / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D000085:Acetates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D012997:Solvents