The construction of glucose biosensor based on platinum nanoclusters-multiwalled carbon nanotubes nanocomposites.
One-step synthesis method was proposed to obtain the nanocomposites of platinum nanoclusters and multiwalled carbon nanotubes (PtNCs-MWNTs), which were used as a novel immobilization matrix for the enzyme to fabricate glucose biosensor. The fabrication process of the biosensor was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, atomic force microscopy and scanning electron microscope. Due to the favorable characteristic of PtNCs-MWNTs nanocomposites, the biosensor exhibited good characteristics, such as wide linear range (3.0 μM-12.1 mM), low detection limit (1.0 μM), high sensitivity (12.8 μA mM⁻¹), rapid response time (within 6 s). The apparent Michaelis-Menten constant (K(app)(m)) is 2.1 mM. The performance of the resulting biosensor is more prominent than that of most of the reported glucose biosensors. Furthermore, it was demonstrated that this biosensor can be used for the assay of glucose in human serum samples.
Key Lab of Chongqing Modern Analytical Chemistry, Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Cheng Yan
CY
Wang
Xing Rong
XR
Tan
Shi Hong
SH
Chen
Fang Xin
FX
Hu
Hua An
HA
Zhong
Yu
Y
Zhang
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008839:Microelectrodes; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry; D010984:Platinum / Q000737:chemistry
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase