[Cloning and identification of the variable region gene of mouse anti-human BAFF mAb].
OBJECTIVE
To obtain the variable region gene sequence of heavy and light chain of mouse anti-human BAFF monoclonal antibody (mAb) on base of BAFF mAb which was cloned in our laboratory.


METHODS
The total RNA was extracted from mouse anti-human BAFF mAb hybridoma cell line FMMUB(4);,  and then the RNA was reverse transcribed into cDNA. Specific primers were designed to amplify the targeted gene. The targeted gene fragments were inserted into vectors to construct the clone vectors. The gene sequences were analyzed after identified by positive clones screening and restrictive enzyme digestion.


RESULTS
The variable region gene sequences of mouse anti-human BAFF mAb were obtained.


CONCLUSIONS
The variable region gene sequences of mouse anti-human BAFF mAb will provide experimental basis for further study on constructing engineered antibodies.
Department of Immunology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
Li-ming
LM
Xiao
Jiu-yu
JY
Gong
Chang
C
Wang
Heng-yue
HY
Chen
Xiang-dong
XD
Li
Bo-quan
BQ
Jin
Li-hua
LH
Chen
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology*; D053264:B-Cell Activating Factor / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D053264:B-Cell Activating Factor; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; C505719:TNFSF13B protein, human