The ratio of sTfR/ferritin is associated with the expression level of TfR in rat bone marrow cells after endurance exercise.
Currently, it is unclear which index of haematological parameters could be used to most easily monitor iron deficiency during endurance training. To address this question, 16 male Wistar rats were randomly assigned to two groups: a sedentary group (n = 8) and an exercised group (n = 8). Initially, animals in the exercise group started running on a treadmill at a rate of 30 m/min, on a 0% grade, for 1 min/session. Running time was gradually increased by 2 min/day. The training plan was one session per day during the initial 2 weeks and two sessions per day during the third to ninth week. At the end of the 9-week experiment, we analysed the blood of the experimental animals for haemoglobin levels, erythrocyte numbers, haematocrit, serum iron levels, total iron binding capacity, transferrin saturation, serum ferritin levels and soluble transferrin receptor (sTfR) levels, and we calculated the ratio of sTfR/ferritin. Erythrocyte numbers, haemoglobin levels and haematocrit values were decreased after 9 weeks of exercise, but sTfR and sTfR/ferritin values were increased (P < 0.01 or P < 0.05). The training regime significantly increased TfR mRNA levels in the bone marrow cells of the exercised rats compared with the sedentary group (1.8 ± 0.5 vs. 1.1 ± 0.2, P < 0.01). These results revealed a significant correlation between TfR levels in the bone marrow cells and the ratio of sTfR/ferritin (r = 0.517; P < 0.01) and sTfR levels (r = 0.206; P < 0.05) in sedentary and exercised rats. In conclusion, we show that sTfR indices and the ratio of sTfR/ferritin could be useful indicators for monitoring iron deficiency during endurance training.
Sport Biological Center, China Institute of Sport Science, General Administration of Sport, No.11 Tiyuguan Road, Dongcheng District, Beijing, China, 100061.
Ye
Y
Tian
Jiexiu
J
Zhao
Binxiu
B
Zhao
Qi
Q
Gao
Jincheng
J
Xu
Dongsen
D
Liu
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D005080:Exercise Test; D005293:Ferritins / Q000097:blood*; D015870:Gene Expression; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000097:blood; D000090463:Iron Deficiencies; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D021122:Protein Subunits / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000097:blood* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D006454:Hemoglobins; D021122:Protein Subunits; D011990:Receptors, Transferrin; D005293:Ferritins; D007501:Iron