Nucleic acid templated uncaging of fluorophores using Ru-catalyzed photoreduction with visible light.
Hybridization-based reactions have attracted significant attention. The nucleic acid templated photocatalyzed azide reduction using catalytic amounts of a [Ru(bpy)(2)phen](2+) conjugate is reported. The reaction could be performed with as little as 2% of the Ru nucleic acid probe and was shown to productively unquench 7-azido-coumarin as well as uncage a small molecule.
Institut de Science et Ingénierie Supramoléculaires (ISIS - UMR 7006), Université de Strasbourg - CNRS, 8 allée Gaspard Monge, F67000 Strasbourg, France.
Manuel
M
Röthlingshöfer
Katarzyna
K
Gorska
Nicolas
N
Winssinger
D001386:Azides / Q000737:chemistry; D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D008027:Light; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D010084:Oxidation-Reduction; D055668:Photochemical Processes; D012428:Ruthenium / Q000737:chemistry*
D001386:Azides; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012428:Ruthenium