Prokaryotic diacylglycerol kinase and undecaprenol kinase.
Prokaryotic diacylglycerol kinase (DAGK) and undecaprenol kinase (UDPK) are the lone members of a family of multispan membrane enzymes that are very small, lack relationships to any other family of proteins-including water soluble kinases-and exhibit an unusual structure and active site architecture. Escherichia coli DAGK plays an important role in recycling diacylglycerol produced as a by-product of biosynthesis of molecules located in the periplasmic space. UDPK seems to play an analogous role in gram-positive bacteria, where its importance is evident because UDPK is essential for biofilm formation by the oral pathogen Streptococcus mutans. DAGK has also long served as a model system for studies of membrane protein biocatalysis, folding, stability, and structure. This review explores our current understanding of the microbial physiology, enzymology, structural biology, and folding of the prokaryotic DAGK family, which is based on over 40 years of studies.
Department of Biochemistry and Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232, USA.
Wade D
WD
Van Horn
Charles R
CR
Sanders
D020134:Catalytic Domain; D019852:Diacylglycerol Kinase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000201:enzymology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000201:enzymology; D017510:Protein Folding
D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; C021454:undecaprenol kinase