DMBA induces deregulation of miRNA expression of let-7, miR-21 and miR-146a in CBA/CA mice.
BACKGROUND
7,12-Dimethylbenz(α)anthracene (DMBA) is a carcinogen capable of inducing various types of tumors.


METHODS
We investigated the effect of DMBA on micro-RNA expression in CBA/CA H2(k) inbred mice after 24 hours and one week from exposure.


RESULTS
Expression levels of miR-21, miR-146a and let-7a were significantly higher in the vital organs of the mice 24 hours after DMBA exposure compared to those of the controls. On the other hand, a significant down-regulation of the miRNAs was observed seven days after DMBA administration.


CONCLUSIONS
Based on our data, DMBA has an impact on the expression of miR-21, let-7a and miR-146a genes. The altered micro-RNA expression can be regarded as an early effect of exposure to chemical carcinogens. To our knowledge, this is the first study of miRNA modulation caused by DMBA in non-malignant tissues.
Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs, 12 Szigeti str., 7624 Hungary. krisztina.juhasz01@gmail.com
Krisztina
K
Juhász
Katalin
K
Gombos
Mónika
M
Szirmai
Péter
P
Révész
Ingrid
I
Magda
Katalin
K
Gocze
István
I
Ember
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D002273:Carcinogens; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; C534190:Mirn146 microRNA, mouse; C542687:mirnlet7 microRNA, mouse; D015127:9,10-Dimethyl-1,2-benzanthracene