[Study on PLB induction and proliferation of Dendrobium officinale].
OBJECTIVE
To study on the optimal medium ingredients for PLB induction and proliferation of Dendrobium officinale.


METHODS
Seed embryos of Dendrobium officinale were cultivated in MS medium as the basic medium, along with different plant hormones like 6-BA, NAA,2,4-D and KT or their combinations added with organic additives like PE, BE, AE and CM.


RESULTS
BA and NAA combination was not conductive to germination and the germination ratio was even lower than that of MS medium; 10% PE and CM was beneficial to PLB induction; 2,4-D was not conductive to growth and proliferation; A certain concentration of BA, KT and NAA was beneficial to PLB proliferation; KT at 1.0 mg/L recorded the highest 40 d PLB proliferation times at 9.0; PE, CM and AE could promote the PLB proliferation at different levels, among which 10% CM was the most effective.


CONCLUSIONS
The optimized medium ingredients suitable for PLB induction are MS +10% CM +1.0g/L AC; The optimized medium ingredients suitable for PLB proliferation are MS + 1.0 mg/L KT + 0.2 mg/L NAA + 10% CM.
Guangzhou University of Chinese Medicine, Guangzhou 510006, China. zhanggf1026@yahoo.com.cn
Gui-Fang
GF
Zhang
Jie-Min
JM
Guan
Song
S
Huang
Hong-Yuan
HY
Liu
Xing-Hua
XH
Liu
Cai-Xia
CX
Zhu
Xiao-Ping
XP
Lai
D015084:2,4-Dichlorophenoxyacetic Acid; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D031667:Dendrobium / Q000254:growth & development*; D018525:Germination / Q000187:drug effects; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018547:Plant Stems / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*; D012639:Seeds / Q000254:growth & development
D003470:Culture Media; D009280:Naphthaleneacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid; D007701:Kinetin