Avian paramyxovirus serotypes 2-9 (APMV-2-9) vary in the ability to induce protective immunity in chickens against challenge with virulent Newcastle disease virus (APMV-1).
The avian paramyxoviruses (APMVs) belong to the genus Avulavirus of family Paramyxoviridae. The APMVs are classified into nine serotypes on the basis of hemagglutination inhibition (HI) and neuraminidase inhibition (NI) assays, although some serologic cross-reaction exists. Newcastle disease virus (NDV), which constitutes serotype 1 (APMV-1), is an important pathogen of poultry, but the pathogenic potential of the other APMV serotypes is poorly understood. Although antibodies to APMV -2 to -9 are prevalent in chickens, the effect of prior exposure to these serotypes on susceptibility to NDV infection and disease was not known. In the present study, chickens were immunized with APMV-2 to -9 by the oculo-nasal route and later were challenged by the same route with a highly virulent strain of NDV. Among APMV-2 to -9, only APMV-3 induced serum antibodies that cross-reacted significantly with NDV and had significant NDV-neutralizing activity in vitro. In mock-immunized chickens, challenge NDV replicated throughout the respiratory tract as well as in the brain, spleen, and enteric tract. In contrast, in APMV-3-immunized chickens, challenge NDV replication was restricted to the upper respiratory tract and trachea. Some of the other APMVs also induced partial restriction of challenge NDV replication: for example, challenge NDV was not detected in the brains of APMV-9-immunized chickens, and shedding from the respiratory tract was reduced in chickens immunized with APMV-8 and -9. All of the chickens immunized with APMV-3 survived the NDV challenge; with APMV-2, -7, -8, and -9 the percentage survival was 30%, 20%, 20%, and 52.5%, respectively; whereas none of the chickens immunized with APMV-4, -5, or -6 survived. These results show that prior infection of chickens with APMV-3 induced substantial protection against NDV challenge, whereas prior infection with APMV-2, -7, -8, and -9 can alter subsequent NDV infection. The induction of NDV-neutralizing antibodies was a marker for efficient protection, but partial protection also was observed in their absence.
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.
Baibaswata
B
Nayak
Flavia Militino
FM
Dias
Sachin
S
Kumar
Anandan
A
Paldurai
Peter L
PL
Collins
Siba K
SK
Samal
D000281:Administration, Intranasal; D060433:Administration, Ophthalmic; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D018109:Avulavirus / Q000145:classification / Q000276:immunology*; D001921:Brain / Q000821:virology; D002645:Chickens; D056738:Cross Protection; D003429:Cross Reactions; D004195:Disease Models, Animal; D041981:Gastrointestinal Tract / Q000821:virology; D009521:Newcastle Disease / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D012137:Respiratory System / Q000821:virology; D012703:Serotyping; D013154:Spleen / Q000821:virology; D016019:Survival Analysis; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology; D017201:Virus Shedding
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014765:Viral Vaccines