Highly sensitive trivalent copper chelate-H2O2 system for CE-chemiluminescent detection of luminol-type compounds.
Luminol-type compounds can be used as chemiluminescent (CL) derivatization reagents for amines, carboxylic acids and protein. Copper chelate diperiodatocuprate(III) (K5[Cu(HIO6)2], DPC) was synthesized by complexation of copper at trivalent oxidation state and periodate in a strong basic medium. It was found that DPC can greatly enhance the reaction between luminol-type compounds and H2O2 to produce very strong CL emission. Based on this fact, a rapid CE method combined with high-sensitive end-column CL detection was established to simultaneously analyze luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) with wide concentration range of 3.0-300 nmol/L in 5 min. The RSDs of the signal intensity and the migration time were less than 3.9 and 7.0% for a standard sample containing 100 nmol/L luminol and ABEI (n=5), respectively. The investigation implies that DPC is a promising sensitizer for CE-CL detection of a great variety of biomolecules and drugs in biological samples after derivatization using luminol derivatives.
Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, PR China. fuzf@swu.edu.cn
Zhifeng
Z
Fu
Zongyun
Z
Li
Haoyue
H
Xie
Tao
T
Li
Cuifang
C
Li
D003300:Copper / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007452:Iodates / Q000737:chemistry*; D049408:Luminescent Agents / Q000032:analysis / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007452:Iodates; D049408:Luminescent Agents; C561085:N-(4-aminobutyl)-N-ethylisoluminol; C524140:potassium diperiodatocuprate(III); D008165:Luminol; D003300:Copper; D006861:Hydrogen Peroxide