Acid-insoluble human dentin as carrier material for recombinant human BMP-2.
The aim of this study is to estimate the increase of bone-inductive potency by human demineralized dentin matrix (DDM) with recombinant human bone morphogenetic protein-2 (BMP-2). Human teeth were crushed, completely demineralized in 0.6M HCl, and freeze-dried. The tooth-derived material is called DDM. The shape of DDM was a particle type and its size varied from 0.4 to 0.8 mm. The BMP-2 dose-dependent study in the rat subcutaneous tissues demonstrated that the volume of induced bone and marrow increased at a dose-dependent manner. The time-course study of bone induction by the BMP-2 (5.0 μg)/DDM (70 mg) was estimated histologically and biochemically. Histological findings showed that the BMP-2/DDM increased bone and marrow sequentially between the DDM particles. Calcium content in the BMP-2/DDM-induced tissue was compatible to the histological findings. ALP activity in the BMP-2/DDM showed a maximal value at 1 week and gradually decreased. The morphometric analysis demonstrated that the BMP-2/DDM showed 66.9%, 79.0% in the volume of bone and marrow, and 32.4%, 21.0% in that of DDM at 8, 32 weeks, respectively. We confirmed that BMP-2 significantly accelerated bone formation in the acid-insoluble human-dentin carriers. These results indicate that human DDM should be an effective carrier for delivering BMP-2 and superior scaffold for bone-forming cells.
Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Kanazawa 1757 Tobetsu-Chou, Ishikari-Gun 061-0293, Japan. murata@hoku-iryo-u.ac.jp
Masaru
M
Murata
Daisuke
D
Sato
Jun
J
Hino
Toshiyuki
T
Akazawa
Junichi
J
Tazaki
Katsutoshi
K
Ito
Makoto
M
Arisue
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D003804:Dentin / Q000737:chemistry* / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D008422:Materials Testing; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; C093073:Demineralized Dentin Matrix; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2