Human papillomavirus-16 (HPV-16) infection association with CIAP-2 expression in head and neck cancer.
Human papillomavirus (HPV-16) E6 proteins inhibit apoptosis in both p53-dependent and p53-independent manners. So it was relevant to assess the impact of such infection on head and neck cancers and its relation to the inhibitors of apoptosis (IAPs). CIAP2 is one of these IAPs that is postulated to upregulated by E6 proteins of HPV-16 by amplification of the locus bearing it in many tissues. In this study, we aimed to search for the amplification of the locus bearing CIAP-2 and its relation to HPV-16 in head and neck cancer that may have prognostic and therapeutic impacts on these patients. Total 30 patients diagnosed as head and neck cancer (2 tissue samples were taken from each patient: from the tumor and from the safety margin). All samples were subjected to qualitative polymerase chain reaction analysis for HPV-16 and qualitative and semiquantitative reverse transcription polymerase chain reaction of CIAP-2. There was a significant association between HPV-16 and CIAP2 positivity and head and neck tumors (P=0.01). CIAP-2 expression in malignant tissues was highly associated with HPV-16 infection with 73.9% sensitivity and absolute specificity.
Medical Biochemistry Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381, Egypt. amal.bary@gmail.com
Amal
A
Mansour
Marwa
M
Ali
Hussein
H
Helmy
Samar
S
Kassim
D000293:Adolescent; D000328:Adult; D000368:Aged; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; D002294:Carcinoma, Squamous Cell / Q000209:etiology; D005260:Female; D006258:Head and Neck Neoplasms / Q000209:etiology* / Q000821:virology; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects; D000077195:Squamous Cell Carcinoma of Head and Neck; D044767:Ubiquitin-Protein Ligases
D051034:Inhibitor of Apoptosis Proteins; C494832:BIRC3 protein, human; D000075747:Baculoviral IAP Repeat-Containing 3 Protein; D044767:Ubiquitin-Protein Ligases