PEG-tethered guanosine acetal conjugates for the enzymatic synthesis of modified RNA.
A short and efficient synthetic route to guanosine monophosphate conjugates connected to a protected aldehyde group via a flexible polydisperse polyethylene glycol linker is described. These conjugates are enzymatically incorporated into RNA transcripts with 52% yield. The resulting aldehyde-modified RNA gave full conversion in the reaction with biotin hydrazide, demonstrating the utility of the method.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany.
Florentine
F
Wahl
Andres
A
Jäschke
D000080:Acetals / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012313:RNA / Q000096:biosynthesis*; D014764:Viral Proteins / Q000737:chemistry*
D000080:Acetals; D014764:Viral Proteins; D011092:Polyethylene Glycols; D012313:RNA; D006157:Guanosine Monophosphate; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases