OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants.
Dephosphorylation plays a pivotal role in regulating plant growth, development and abiotic/biotic stress responses. Here, we characterized a plant and fungi atypical dual-specificity phosphatase (PFA-DSP) subfamily member, OsPFA-DSP1, from rice. OsPFA-DSP1 was determined to be a functional protein tyrosine phosphatase (PTP) in vitro using phosphatase activity assays. Quantitative real-time PCR and GENEVESTIGATOR analysis showed that OsPFA-DSP1 mRNA was induced by drought stress. Transfection of rice protoplasts showed that OsPFA-DSP1 accumulated in both the cytoplasm and nucleus. Ectopic overexpression of OsPFA-DSP1 in tobacco increased sensitivity to drought stress and insensitivity to ABA-induced stomatal closure and inhibition of stomatal opening. Furthermore, overexpression of OsPFA-DSP1 in rice also increased sensitivity to drought stress. These results indicated that OsPFA-DSP1 is a functional PTP and may act as a negative regulator in drought stress responses.
Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 510275 Guangzhou, People's Republic of China.
Bing
B
Liu
Jieqiong
J
Fan
Yang
Y
Zhang
Peiqiang
P
Mu
Peng
P
Wang
Jianbin
J
Su
Huihuang
H
Lai
Shaowu
S
Li
Dongru
D
Feng
Jinfa
J
Wang
Hongbin
H
Wang
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017124:Conserved Sequence / Q000235:genetics; D055864:Droughts; D054637:Dual-Specificity Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007477:Ions; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D011994:Recombinant Proteins / Q000302:isolation & purification; D013312:Stress, Physiological / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology; D013379:Substrate Specificity / Q000187:drug effects; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
D007477:Ions; D008670:Metals; D010940:Plant Proteins; D011994:Recombinant Proteins; D054637:Dual-Specificity Phosphatases