Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat.
We used gametocidal (Gc) chromosomes 2C and 3C(SAT) to dissect barley 2H added to common wheat. The Gc chromosome induces chromosomal breakage resulting in chromosomal aberrations in the progeny of the 2H addition line of common wheat carrying the monosomic Gc chromosome. We conducted in situ hybridization to select plants carrying structurally rearranged aberrant 2H chromosomes and characterized them by sequential C-banding and in situ hybridization. We established 66 dissection lines of common wheat carrying single aberrant 2H chromosomes. The aberrant 2H chromosomes were of either deletion or translocation or complicated structural change. Their breakpoints were distributed in the short arm (2HS), centromere (2HC) and the long arm (2HL) at a rough 2HS/2HC/2HL ratio of 2:1:2. We conducted PCR analysis of the 66 dissection lines using 115 EST markers specific to chromosome 2H. Based on the PCR result, we constructed a physical or cytological map of chromosome 2H that were divided into 34 regions separated by the breakpoints of the aberrant 2H chromosomes. Forty-seven markers were present in 2HS and 68 in 2HL. We compared the 2H cytological map with a previously reported 2H genetic map using 44 markers that were used in common to construct both maps. The order of markers in the distal region was the same on both maps but that in the proximal region was somewhat contradictory between the two maps. We found that the markers distributed rather evenly in the genetic map were actually concentrated in the distal regions of both arms as revealed by the cytological map. We also recognized an EST-marker or gene-rich region in the 2HL interstitial region slightly to the telomere.
Laboratory of plant genetics, Graduate School of Agriculture, Kyoto University, Japan.
Giri Prasad
GP
Joshi
Shuhei
S
Nasuda
Takashi R
TR
Endo
D002503:Centromere / Q000235:genetics; D002871:Chromosome Banding / Q000379:methods; D019457:Chromosome Breakage; D002872:Chromosome Deletion; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018745:Genome, Plant; D001467:Hordeum / Q000166:cytology / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D020161:Physical Chromosome Mapping; D016615:Telomere / Q000235:genetics; D014178:Translocation, Genetic; D014908:Triticum / Q000166:cytology / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers