Can Chlorella pyrenoidosa be a bioindicator for hazardous solid waste detoxification?
Four kinds of solid waste residue (SWR, S1 to S4) from different stages in a sequential detoxification process were chosen. The biotoxicity of the leachates from S1 to S4 was tested by Chlorella pyrenoidosa. The growth inhibition, the chlorophyll a (chla) and chlorophyll b (chlb) concentrations, and the ultrastructural morphology of cells of C. pyrenoidosa were studied. It shows that the growth inhibition of C. pyrenoidosa significantly increased with increasing leachate concentration when exposed to the leachates from S1, S2, S3, and S4, respectively. It well reflects the toxicity difference of leachate from SWR at different treatment stages, namely S1>S2>S3>S4. Correspondingly, the chla and chlb concentrations of C. pyrenoidosa increased gradually as SWR was treated deeply. Leachate disrupted chlorophyll synthesis and inhibited cell growth. The changing of the ultrastructural morphology of cells under different leachate exposures, such as volume of chloroplasts and quantity of thylakoids reducing, confirmed the toxicity decrease of leachates from different stages. C. pyrenoidosa is a good bioindicator for hazardous solid waste detoxification. The EC(50) at difference scenarios also suggests that it was feasible to estimate ecological toxicity of leachates to C. pyrenoidosa after exposure times of 72h. C. pyrenoidosa can be introduced to evaluate the effect of hazardous solid waste disposal by biotoxicity assessment.
College of Quality and Safety Engineering, China Jiliang University, Hangzhou 310018, China. hulif127@163.com
Li-Fang
LF
Hu
Yu-Yang
YY
Long
Dong-Sheng
DS
Shen
Chen-Jing
CJ
Jiang
D000814:Aniline Compounds / Q000009:adverse effects / Q000032:analysis; D001151:Arsenic / Q000009:adverse effects / Q000032:analysis; D002708:Chlorella / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development; D004784:Environmental Monitoring / Q000379:methods*; D006256:Hazardous Waste / Q000009:adverse effects* / Q000032:analysis; D012037:Refuse Disposal / Q000379:methods* / Q000592:standards
D000814:Aniline Compounds; D006256:Hazardous Waste; C008625:nitroaniline; D001151:Arsenic; C023650:aniline