[Effects and molecular mechanisms of inhibiting FOXM1 expression on ovarian cancer cell line SKOV3 by Lentiviral vector].
OBJECTIVE
To investigate the effects and possible molecular mechanisms of inhibiting FOXM1 expression on SKOV3 cells by lentiviral vector targeting FOXM1 shRNA.


METHODS
SKOV3 cells were infected by lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection (MOI) of 20,  then growth curve of SKOV3 cells was determined by MTT assay,  cell cycle was analysed by flow cytometry(FCM),  and the expression of mRNA and protein of FOXM1,  Cyclin D1,  PLK1 by Real time PCR and Western blot.


RESULTS
Lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection (MOI) of 20 could significantly inhibit the growth of SKOV3 cells. After infected by lentiviral vector targeting FOXM1 shRNA,  the G(0);/G(1); phase cells increased and the S-phase cells decreased,  and the expression of mRNA and protein of FOXM1,  Cyclin D1, PLK1  of SKOV3 cells were significantly down-regulated.


CONCLUSIONS
Inhibiting FOXM1 expression has a significantly effect of inhibiting proliferation on SKOV3 cells. Blocking SKOV3 cells in the G(0);/G(1); phase by down-regulating the expression of Cyclin D1,  PLK1 protein may be its mechanism.
Department of Obstetrics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. cgq@19841001@126.com
Guo-qing
GQ
Chen
Zhen-wei
ZW
Yao
Hong-bo
HB
Qi
Hua
H
Zhang
Yi
Y
Luo
Xing-fa
XF
Qin
D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D019938:Cyclin D1; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1; C107585:FOXM1 protein, human