New microsatellite markers for garlic, Allium sativum (Alliaceae).
OBJECTIVE
A new set of microsatellite or simple sequence repeat (SSR) markers for garlic, an important medicinal spice, was developed to aid studies of genetic diversity and to define efficient strategies for germplasm conservation.


RESULTS
Using a (CT)(8)- and (GT)(8)-enriched library, a total of 16 SSR loci were developed and optimized in garlic. Ten loci were found to be polymorphic after screening 75 accessions. The parameters used to characterize the loci were observed and expected heterozygosity, number of alleles, Shannon Index, and polymorphism information content (PIC). A total of 44 alleles were identified, with an average of 4.4 alleles per loci. The vast majority of loci were moderate to highly informative according to PIC and the Shannon Index.


CONCLUSIONS
The new SSR markers have the potential to be informative tools for genetic diversity, allele mining, mapping and associative studies, and in the management and conservation of garlic collections.
Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz," Universidade de São Paulo, Piracicaba, Brazil.
Camila P
CP
Cunha
Eulália S S
ES
Hoogerheide
Maria I
MI
Zucchi
Mariza
M
Monteiro
José B
JB
Pinheiro
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005737:Garlic / Q000145:classification / Q000235:genetics*; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D010946:Plants, Medicinal; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers