Protein secondary structure imaging with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy.
Protein secondary structures in human hair have been studied with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS peak-shift mapping method has been developed and applied to hair samples with and without treatments by chemical reduction and mechanical extension. It clearly visualizes the treatment induced changes in protein secondary structures and their spatial distributions. Using the new imaging technique, we found a multilayered structure in the human hair cortex.
Analytical Science Research Laboratories, Kao Corporation, Akabane 2606, Ichikai, Haga, Tochigi 321-3497, Japan.
Kotatsu
K
Bito
Masanari
M
Okuno
Hideaki
H
Kano
Shihomi
S
Tokuhara
Satoru
S
Naito
Yoshinori
Y
Masukawa
Philippe
P
Leproux
Vincent
V
Couderc
Hiro-o
HO
Hamaguchi
D002470:Cell Survival; D006197:Hair / Q000166:cytology; D006801:Humans; D008297:Male; D055595:Mechanical Phenomena; D057054:Molecular Imaging / Q000379:methods*; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman / Q000379:methods*