A new and convenient approach for the preparation of β-cyanoethyl protected trinucleotide phosphoramidites.
Herein we report a convenient approach for the preparation of fully protected trinucleotide synthons to be used for the synthesis of gene libraries. The trinucleotide synthons bear β-cyanoethyl groups at the phosphate residues, and thus can be used in standard oligonucleotide synthesis without additional steps for deprotection and work-up.
Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str., 4, 17487 Greifswald, Germany.
Matthäus
M
Janczyk
Bettina
B
Appel
Danilo
D
Springstubbe
Hans-Joachim
HJ
Fritz
Sabine
S
Müller
D003485:Cyanates / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010766:Phosphorylation
D003485:Cyanates; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite