Spectrophotometric detection of tyrosinase activity based on boronic acid-functionalized gold nanoparticles.
A spectrophotometric method for the detection of tyrosinase activity is developed by utilizing the product-triggered aggregation of boronic acid-functionalized gold nanoparticles. Based on the changes of absorbance in UV-visible spectra, the assay shows extremely high sensitivity and lowered limit of detection of 1 × 10(-10) u mL(-1).
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Sa
S
Li
Langyong
L
Mao
Yaping
Y
Tian
Joseph
J
Wang
Nandi
N
Zhou
D001897:Boronic Acids; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001897:Boronic Acids; D006046:Gold; D014442:Monophenol Monooxygenase