Formation of supramolecular structures of a native-like protein in the presence of amphiphilic peptides: Variations in aggregate morphology.
A striking potential of the amphiphilic dipeptides, Arg-Phe or Asp-Phe, to induce aggregation of a model protein, alcohol dehydrogenase in its native-like state, has been demonstrated under physiologically relevant conditions, using dynamic light scattering, fluorescence spectroscopy, circular dichroism, transmission electron- and atomic force microscopy. The peptide action resulted in accumulation of a variety of morphologically distinct supramolecular structures profoundly differing from those generated by the heat-induced aggregation at the early stages of the process, when amyloid fibril assemblies were not detectable. The biogenic amphiphilic agents are suggested to act as regulators of structural transformations of native-like proteins.
A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect, 33, 119071 Moscow, Russia.
Natalya
N
Artemova
Vita
V
Stein-Margolina
Ekaterina
E
Smirnova
Bella
B
Gurvits
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation / Q000187:drug effects*; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force; D046912:Multiprotein Complexes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D017510:Protein Folding; D011498:Protein Precursors / Q000737:chemistry* / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors; D015003:Yeasts / Q000201:enzymology
D046912:Multiprotein Complexes; D010455:Peptides; D011498:Protein Precursors; D013501:Surface-Active Agents; D007768:Lactalbumin; D000426:Alcohol Dehydrogenase; C421864:hen egg lysozyme; D009113:Muramidase