Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice.
Envenomation by Hemiscorpius lepturus (H. lepturus) is associated with local necrosis, followed by systemic manifestations. In this work the LD₅₀ of H. lepturus venom were determined by subcutaneous (SC) injection in white Balb/c mice (5 mg/kg). Histopathological alterations in organs such as kidney, heart, liver, lungs, stomach and intestine were determined in 3, 6, 12 and 24 h following experimental (SC) envenoming injection of one LD ₅₀ of the venom in Balb/c mice. Histological studies showed degenerative changes in the kidney with disorganized glomeruli and necrotic tubular in 3 h and reached to its climax in 6 h. Myocardium showed massive myocytolysis with interstitial necrosis in 3 h and reached to its peak after 6 h past envenoming. Bowels showed edema of lamina propria and slight villous necrosis. The enzymatic activities of creatine kinase (CK) and lactate dehydrogenase (LDH) were significantly increased in the serum in 9 h. No necrotic lesion observed in lungs and liver. The results indicate that the venom of H. lepturus is a highly cytotoxic, and induces massive tissue damages in specific organs, starting from the heart and kidney as the first target in 3 h and ends to the bowels in 6 h post envenomation.
Institute of Standards and Industrial Researches of Iran, Karaj, Iran.
Mojgan
M
Heidarpour
Emna
E
Ennaifer
Hamed
H
Ahari
Najet
N
Srairi-Abid
Lamia
L
Borchani
Ghader
G
Khalili
Hossein
H
Amini
Amir Ali
AA
Anvar
Samir
S
Boubaker
Mohamed
M
El-Ayeb
Delavar
D
Shahbazzadeh
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000473:pathology*; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D007279:Injections, Subcutaneous; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000473:pathology*; D012604:Scorpion Venoms / Q000633:toxicity*; D012605:Scorpions
D012604:Scorpion Venoms; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase