β4 integrin marks interstitial myogenic progenitor cells in adult murine skeletal muscle.
Skeletal muscle growth and its regeneration following injury rely on myogenic progenitor cells, a heterogeneous population that includes the satellite cells and other interstitial progenitors. The present study demonstrates that surface expression of β4 integrin marks a population of vessel-associated interstitial muscle progenitor cells. Muscle β4 integrin-positive cells do not express myogenic markers upon isolation. However, they are capable of undergoing myogenic specification in vitro and in vivo: β4 integrin cells differentiate into multinucleated myotubes in culture dishes and contribute to muscle regeneration upon delivery into diseased mice. Subfractionation of β4 integrin-expressing cells based on CD31 expression does not further enrich for myogenic precursors. These findings support the expression of β4 integrin in interstitial, vessel-associated cells with myogenic activity within adult skeletal muscle.
Program in Genomics, Division of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
Kalliopi
K
Liadaki
Juan Carlos
JC
Casar
McKenzie
M
Wessen
Eric S
ES
Luth
Susan
S
Jun
Emanuela
E
Gussoni
Louis M
LM
Kunkel
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D039663:Integrin beta4 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D018482:Muscle, Skeletal / Q000098:blood supply / Q000294:innervation / Q000473:pathology; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
D015415:Biomarkers; D039663:Integrin beta4; D049452:Green Fluorescent Proteins