[T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens].
The genetic transformation mediated by Agrobacterium tumefaciens has been widely applied to research of transgenic plants. As the vector of the exotic genes, the integration patterns of T-DNA fragments affects not only transformation efficiency and stability, but also expression properties of the transgenes. This review summaries the two major patterns and the rules of T-DNA integration in Agrobacterim-mediated transformation, rules of T-DNA mediated by Agrobacterium tumefaciens, as well as research tools for flanking sequence amplification. It is attempted to provide references for researches on transformation and T-DNA integration mutation mediated by Agrobacterium tumefaciens.
Maize Research Institute, Sichuan Agricultural University, Chengdu, China. myyanglin1986@21cn.com
Lin
L
Yang
Feng-Ling
FL
Fu
Feng-Ling
FL
Fu
Wan-Chen
WC
Li
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA