Encapsulation and survival of a chondrocyte cell line within xanthan gum derivative.
A chemical derivative of xanthan gum polysaccharide is investigated as a new artificial matrix for the encapsulation of chondrocytic cells. Toward this goal, a novel micro-droplet generator is developed to produce microcapsules. Microcapsules with an average diameter of 500 µm, smooth surface, and homogeneous size distribution are obtained. ATDC5 cells encapsulated in carboxymethyl xanthan (CMX) microcapsules remain viable and are observed to proliferate for prolonged culture periods with enhanced metabolic activity. Furthermore, retention of the chondrogenic phenotype is exhibited by the cells within CMX, suggesting the ability of this material to be applied in cell-delivery therapies.
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal. ana.mendes@dep.uminho.pt
Ana C
AC
Mendes
Erkan T
ET
Baran
Rui C
RC
Pereira
Helena S
HS
Azevedo
Rui L
RL
Reis
D000818:Animals; D002214:Capsules / Q000138:chemical synthesis* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018914:Cells, Immobilized; D019902:Chondrocytes / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis* / Q000494:pharmacology
D002214:Capsules; D004337:Drug Carriers; D011135:Polysaccharides, Bacterial; C002563:xanthan gum