NaDDBS as a dispersion agent for multiwalled carbon nanotubes in capillary EKC separation of nucleotides.
A novel pseudostationary phase (PSP) of multiwalled carbon nanotubes (MWCNTs) dispersed with sodium dodecylbenzenesulfonate (NaDDBS) was used for the EKC separation of nucleotides. NaDDBS has a long hydrophobic chain and a benzylsulfonate group. It suspends more MWCNTs (about 100-fold) than SDS, and the π-π interaction between the benzene ring of NaDDBS and MWCNTs prolongs the slurry suspension time. Using NaDDBS as a surfactant can reduce the required amount of MWCNTs and decrease the baseline noise. To produce a stable suspension, the optimum ratio (w/w) of MWCNTs to NaDDBS was investigated with turbidimetry. In this context, several parameters affecting EKC separation were studied, including buffer pH, composition, concentration, and the organic modifier. Use of NaDDBS (8 mg/L)/MWCNTs (0.8 mg/L) as the PSP in a phosphate buffer (30 mM, pH 8) yielded complete resolution of seven geometric isomers of a nucleoside monophosphate. In stacking mode, with 10% MeOH in the sample plug, the mixture of nucleoside mono-, di-, and tri-phosphates was satisfactorily separated in phosphate buffer (50 mM, pH 9). The results indicate that nucleotides with bases containing more electron-withdrawing groups interact more strongly with MWCNTs. The system has been used to separate oligonucleotides, and to analyze nucleotides in a complex matrix sample.
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Yu-Fang
YF
Hsieh
Bao-Yu
BY
Huang
Ching-Piao
CP
Liu
Chuen-Ying
CY
Liu
D001557:Benzenesulfonates / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006157:Guanosine Monophosphate / Q000737:chemistry / Q000302:isolation & purification; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification*; D011066:Poly C / Q000737:chemistry / Q000302:isolation & purification
D001557:Benzenesulfonates; D037742:Nanotubes, Carbon; D009711:Nucleotides; D011066:Poly C; C001114:dodecylbenzenesulfonic acid; D006157:Guanosine Monophosphate