Immunohistochemical expression of RANKL, RANK and OPG in gingival tissue of patients with periodontitis.
OBJECTIVE
To evaluate the expression of the receptor activator of NF-κB (RANK), the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG), in the gingival tissue of patients with periodontitis.


METHODS
Gingival tissue was obtained from 14 systemically healthy subjects with chronic periodontitis during conventional periodontal surgery. Immunohistochemistry was used to detect the expression of RANK, RANKL and OPG in the oral and periodontal pocket epithelium as well as in the connective tissue cells.


RESULTS
RANKL was negatively expressed in both oral and periodontal pocket epithelium. OPG was also negative or weakly positive in the whole epithelium. RANK showed moderate/strong positive staining mainly in the basal and suprabasal layer of oral and periodontal pocket epithelium. In most of the cases, more than 60% of the inflammatory cell infiltrate stained for RANK and RANKL. In these cases the intensity of the stained cells ranged from moderate-to-strong. In less than half of the cases, OPG was positive in more than 60% of the stained cells of the inflammatory cell infiltrate.


CONCLUSIONS
The RANK, RANKL and OPG proteins are differentially expressed in periodontal tissues and may play a major role in the bone loss occurring in periodontitis.
Division of Periodontology, School of Dental Medicine, University of Geneva, Switzerland. ekaterini.giannopoulou@unige.ch
Catherine
C
Giannopoulou
Carla Patricia
CP
Martinelli-Klay
Tommaso
T
Lombardi
D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053244:Osteoprotegerin / Q000378:metabolism*; D010518:Periodontitis / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism*
D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C505688:TNFSF11 protein, human