Capillary electrophoresis analysis of isoniazid using luminol-periodate potassium chemiluminescence system.
A rapid and simple capillary electrophoresis method coupled with chemiluminescent (CL) detection was proposed for analysis of isoniazid (ISO) based on the enhancement effect of ISO to CL emission of luminol-periodate potassium reaction. Under the optimal conditions, ISO can be assayed in the range of 7.0 × 10(-7) to 3.0 × 10(-5) g mL(-1) (R(2) = 0.9990) with a limit of detection of 3.0 × 10(-7) g mL(-1) (signal-to-noise ratio of 3). The whole analysis process can be completed within 2.5 min with a theoretical plate number of 6258. The relative standard deviations of the signal intensity and the migration time were 3.1 and 1.4% for a standard sample at 1.0 × 10(-5) g mL(-1) (n = 5), respectively. The presented novel strategy was successfully applied to the determination of ISO in commercial pharmaceutical preparations and spiked human serum samples. Copyright © 2010 John Wiley & Sons, Ltd.
Key Laboratory on Luminescence and Real-Time Analysis (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China.
Ying
Y
Liu
Zhifeng
Z
Fu
Lin
L
Wang
D000995:Antitubercular Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007538:Isoniazid / Q000032:analysis*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*
D000995:Antitubercular Agents; D017680:Potassium Compounds; D010504:Periodic Acid; D008165:Luminol; C037192:potassium periodate; D007538:Isoniazid