[Analysis of the mechanism of apoptosis induction by PSK].
Previously, we reported that PSK induces apoptosis and growth inhibition in HL60 cells. In this study, we tried to clarify the mechanism of how PSK induces apoptosis. Because several reports suggested that apoptosis of HL60 cells is mediated by activation of p38MAPK, we examined whether p38MAPK is involved in PSK-induced apoptosis. First, we found that PSK induced p38MAPK phosphorylation, which is considered as its activation. Next, we demonstrated that SB203580, inhibitor of p38MAPK, inhibited PSK-induced apoptosis. These results suggest that p38MAPK plays an important role in PSK-induced apoptosis.
Dept. of Digestive and General Surgery, Shimane University Faculty of Medicine.
Noriyuki
N
Hirahara
Takeo
T
Edamatsu
Ayako
A
Fujieda
Masaki
M
Fujioka
Tsutomu
T
Wada
Tsuneo
T
Tanaka
D017209:Apoptosis / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011509:Proteoglycans / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D007093:Imidazoles; D011509:Proteoglycans; D011725:Pyridines; C010770:polysaccharide-K; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580