[Construction of eukaryotic expression vector and package of lentivirus vector encoding human leukocyte antigen-G5].
OBJECTIVE
To construct a lentiviral expression vector carrying HLA-G5.


METHODS
The CDs region of HLA-G5 gene was cloned into the lentiviral vector by restriction endonuclease Nhe I/Not I digestion and T4DNA ligase ligation. After transformation into completent E.coli cells,  the candidate clones were identified by PCR and kidney cell line 293T cells by lipofectamine 2000 to produce the lentiviral particles,  and the viral titer was determined.


RESULTS
The lentiviral vector pCDH-CMV-MCS-EF1-copGFP Vector for  HLA-G5 was constructed successfully,  and the virus in the supernatant reached a titer of TU/mL.


CONCLUSIONS
This research completed the package of lentivirus vector encoding HLA-G5 as a tool for further study.
Department of Gynecology and Obstetrics,  the Chniese PLA General Hospital,  Beijing 100853, China.
Xin-yu
XY
Guo
Feng
F
Jiang
Hong-xi
HX
Zhao
Yuan-qing
YQ
Yao
D001483:Base Sequence; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D057809:HEK293 Cells; D059951:HLA-G Antigens / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008969:Molecular Sequence Data
D059951:HLA-G Antigens