Cell-specific siRNA delivery by peptides and antibodies.
Cellular targeting and intracellular delivery of small interfering RNA (siRNA) remain a critical barrier to the clinical application of RNA interference. This chapter provides an overview of various delivery agents employing protein ligands mediating cell-specific delivery of siRNA. Specifically, the chapter details methodologies for the conjugation of antibody or peptide ligands to i) the cationic peptide-oligo-9-arginine (ii) the polymer poly(lactic-co-glycolic acid) (PLGA) and (iii) a lipid-vesicle (liposome).
Department of Bioengineering and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, South Korea.
Sang-Kyung
SK
Lee
Alyssa
A
Siefert
Jagadish
J
Beloor
Tarek M
TM
Fahmy
Priti
P
Kumar
D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D009928:Organ Specificity; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
D000906:Antibodies; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D008081:Liposomes; D009842:Oligopeptides; D034741:RNA, Small Interfering; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid