Effects of different substrate composition on biosynthesis of polyhydroxybutyrate-co-hydroxyvalerate by recombinant Escherichia coli.
Cupriavidus necator is well known for its ability to accumulate polyhydroxybutyrate (PHB). When supplemented with propionic acid (or sodium propionate) in the growth medium, the bacterium is also able to synthesize polyhydroxybutyrate-co-hydroxyvalerate (PHBV). In order to increase the fraction of 3-hydroxyvalerate (3HV) in PHBV, we cloned the propionate permease gene prpP from C. necator and the propionyl-CoA synthase gene prpE from Cupriavidus taiwanensis and transformed into an Escherichia coli containing phaCAB operon of C. necator. The effects on PHBV accumulation in cells co-expressed with phaCAB and prpE or prpP in the media contained mixed carbon sources (glucose and sodium propionate) were evaluated. The HV fraction in PHBV increased when prpE or prpP was overexpressed in the cells. Concentrations of yeast extracts could also affect the fraction of HV. In addition, when glucose was replaced by sodium pyruvate, sodium succinate, or sodium gluconate, only PHB were detected in the recombinant strains.
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Rd, Chung-Li, 320 Taiwan. ccchien@saturn.yzu.edu.tw
Chih-Ching
CC
Chien
Hsiu-Hsiung
HH
Li
Po-Chi
PC
Soo
Shan-Yu
SY
Chen
Yu-Hong
YH
Wei
Wen-Ming
WM
Chen
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D049921:Cupriavidus / Q000235:genetics / Q000378:metabolism; D020118:Cupriavidus necator / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D010421:Pentanoic Acids / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D003066:Coenzyme A Ligases; D005947:Glucose; D006885:Hydroxybutyrates; D026901:Membrane Transport Proteins; D010421:Pentanoic Acids; D011091:Polyesters; D011422:Propionates; D011994:Recombinant Proteins; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid; C003182:poly-beta-hydroxybutyrate; C514135:sodium propionate; C068072:propionate - CoA ligase