Analysis of aging in Caenorhabditis elegans.
This chapter is dedicated to the study of aging in Caenorhabditis elegans (C. elegans). The assays are divided into two sections. In the first section, we describe detailed protocols for performing life span analysis in solid and liquid medium. In the second section, we describe various assays for measuring age-related changes. Our laboratory has been involved in several fruitful collaborations with non-C. elegans researchers keen on testing a role for their favorite gene in modulating aging (Carrano et al., 2009; Dong et al., 2007; Raices et al., 2008; Wolff et al., 2006). But even with the guidance of trained worm biologists, this undertaking can be daunting. We hope that this chapter will serve as a worthy compendium for those researchers who may or may not have immediate access to laboratories studying C. elegans.
Howard Hughes Medical Institute, Glenn Center for Aging Research, Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Deepti S
DS
Wilkinson
Rebecca C
RC
Taylor
Andrew
A
Dillin
D000375:Aging / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D001681:Biological Assay; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D031204:Caloric Restriction; D045222:Electron Transport Chain Complex Proteins / Q000235:genetics / Q000378:metabolism; D005298:Fertility; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008062:Lipofuscin / Q000096:biosynthesis; D008124:Locomotion; D008136:Longevity; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction / Q000235:genetics*; D015996:Survival Rate
D029742:Caenorhabditis elegans Proteins; D045222:Electron Transport Chain Complex Proteins; D007328:Insulin; D008062:Lipofuscin; D007334:Insulin-Like Growth Factor I