p53 Codon 72 polymorphism in oral exfoliated cells in a Sudanese population.
Earlier studies have investigated the tumor suppressor gene p53 as a co-factor in the development of oral squamous cell carcinoma (OSCC). Our previous studies have indicated that chronic use of Sudanese snuff (toombak) and the presence of human papilloma virus (HPV) may be involved in the high prevalence of OSCC in Sudan. This study investigated the prevalence of p53 codon 72 polymorphism in brush biopsies obtained from a Sudanese population. A total of 174 individuals were included in the study; chronic toombak users (n=152) and non-users (n=22). DNA was extracted from all the samples and genotyped for the codon 72 polymorphism by polymerase chain reaction/restriction fragment length polymorphism. The Arg/Pro genotype was found in 53% of the 174 study participants, compared to 21% found with Arg/Arg and 26% found with Pro/Pro. Stratifying by toombak use, 28 (18%), 45 (29%) and 79 (52%) of the 152 samples from toombak users had Arg/Arg, Pro/Pro and Arg/Pro respectively, compared to 9 (41%), 0 (0%) and 13 (59%) found in the 22 samples from non users. The differences between the samples from toombak users and non users in Arg/Arg and Pro/Pro codon 72 polymorphism and HPV infection were statistically significant (p<0.05). Our study indicated that a high prevalence of the genotype Arg/Pro at the p53 codon 72 may contribute to susceptibility to OSCC, especially in combination with the use of carcinogenic tobacco-specific nitrosamine (TSNA)-rich toombak. Our observations warrant an in-depth study for understanding the role of p53 polymorphism in human oral cancers.
Department of Surgical Sciences, Oral and Maxillofacial Surgery, Medical Faculty, Uppsala University, SE-751 85 Uppsala, Sweden. lars.sand@hotmail.com
Lars
L
Sand
Miranda M
MM
Jalouli
Jamshid
J
Jalouli
Dipak
D
Sapkota
Salah O
SO
Ibrahim
D000293:Adolescent; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics / Q000473:pathology; D003062:Codon / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D009062:Mouth Neoplasms / Q000209:etiology / Q000235:genetics / Q000473:pathology; D009602:Nitrosamines / Q000506:poisoning; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013397:Sudan; D014030:Tobacco, Smokeless / Q000737:chemistry / Q000506:poisoning; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
D003062:Codon; D009602:Nitrosamines; D016159:Tumor Suppressor Protein p53