Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.
The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
Department of Neurobiology and Genetics, Theodor-Boveri Institute, University of Wuerzburg, Wuerzburg, Germany.
Partho
P
Halder
Yi-Chun
YC
Chen
Janine
J
Brauckhoff
Alois
A
Hofbauer
Marie-Christine
MC
Dabauvalle
Urs
U
Lewandrowski
Christiane
C
Winkler
Albert
A
Sickmann
Erich
E
Buchner
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D001921:Brain / Q000378:metabolism*; D029721:Drosophila Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006825:Hybridomas; D047468:Immunoprecipitation; D042541:Intracellular Space / Q000378:metabolism; D013058:Mass Spectrometry; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000941:Antigens; D029721:Drosophila Proteins; C512908:Eps-15 protein, Drosophila; D009419:Nerve Tissue Proteins