Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions.
Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
Ke
K
Xu
Hazen P
HP
Babcock
Xiaowei
X
Zhuang
D000199:Actins / Q000737:chemistry*; D002460:Cell Line; D003599:Cytoskeleton / Q000737:chemistry*
D000199:Actins