A practical strategy to create near-infrared luminescent probes: conversion from fluorescein-based sensors.
Luminescent lanthanide complexes incorporating Yb(3+) and Nd(3+) are attracting much attention as imaging agents, but there have been few practical methods to make responsive sensors with these complexes. Here, we introduce a general strategy to synthesize near-infrared luminescent probes by conjugating a Yb(3+) chelate to established fluorescein-based probes. As the first demonstration, we present a complex, based on the green-emitting probe DAF-4, that responds to nitric oxide (NO) in aqueous solution with a significant increase in luminescence intensity at 980 nm.
Graduate School of Pharmaceutical Sciences, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Takuya
T
Terai
Yasuteru
Y
Urano
Saki
S
Izumi
Hirotatsu
H
Kojima
Tetsuo
T
Nagano
D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D019793:Fluorescein / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D009354:Neodymium / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D014867:Water / Q000737:chemistry; D015018:Ytterbium / Q000737:chemistry
D056831:Coordination Complexes; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D014867:Water; D009354:Neodymium; D009569:Nitric Oxide; D015018:Ytterbium; D019793:Fluorescein