[Study of phospholipid profile of ovarian tumor by high performance liquid chromatography-mass spectrometry].
Ovarian tumor has been paid more and more attention since its influence on women's health and life quality is increasing. Ovarian cancer is one of the three gynecologic cancers, and its mortality is the highest one of them. Phospholipid metabolic profiling method based on high performance liquid chromatography-electrospray mass spectrometry (HPLC/ESI-MS) has been applied in the study of ovarian tumors including benign (B) and malignant (M) ovarian tumors. The data of phospholipid profile collected by HPLC/ESI-MS were transformed and the peak list was obtained with the commercial software automatically. The total differences of phospholipids among M, B and normal (N) groups were found with the orthogonal signal correction and partial least-squares (OSC-PLS). Further, the differential phospholipids were selected according to the S-plot, the variable importance value (VIP > 1) and p (p < 0.05). These phospholipids were plasmalogen phospatidylethanol, phosphatidylcholine, plasmalogen phosphatidylcholine, sphingomyelin and lysophosphatidylcholine. This research provides some new and useful information of what has happened in phospholipids of the women with ovarian tumors.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Sumin
S
Zhao
Yisheng
Y
Wang
Abo
A
Dou
Jing
J
Chen
Xin
X
Lu
Rui
R
Cao
Congjian
C
Xu
Guowang
G
Xu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008244:Lysophosphatidylcholines / Q000097:blood; D055442:Metabolome; D010051:Ovarian Neoplasms / Q000097:blood*; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000097:blood* / Q000145:classification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010743:Phospholipids