[The effect of human placenta-derived mesenchymal stem cells on cord blood CD8(+);T cells activation,  cellcycle and secretion of IL-17].
OBJECTIVE
To study the effect of human placenta-derived mesenchymal stem cells (hPMSCs) on cord blood CD8(+);T cell activation,  cell cycle and secretion of IL-17,  and to provide the theoretical basis for it application in the cell-based therapies.


METHODS
hPMSCs were isolated from mature placenta by the method of digestion. Then hPMSCs were cultured,  expanded in vitro,  and were used in test after the third passage. CD8(+);T cells were sorted from cord blood with immunomagetic beads. FCM was used to analyze the expression of early activation phenotype,  cell cycle of cord blood CD8(+);T cells and cytokine secretion.


RESULTS
CD8(+);T cells stimulated by PHA in the presence of hPMSCs were arrested at G0/G1 phase. The expression of the early activation marker CD25 and CD69 of cord blood CD8(+);T cells was inhibited in the presence of hPMSCs. While,  IL-17secretion of cord blood CD8(+);T cells stimulated by PMA was increased.


CONCLUSIONS
hPMSCs can suppress the activation of cord blood CD8(+);T cells by altering T cell cycle;  up-regulate the level of IL-17 secreted by cord blood CD8(+);T cells.
Department of Immunology, Binzhou Medical University, Yantai 264003, China. wgy1201@163.com
Guo-yan
GY
Wang
Si-ying
SY
Zhang
Guang-yun
GY
Li
Fei-fei
FF
Wang
Hai-bo
HB
DU
Xi-ying
XY
Luan
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002453:Cell Cycle / Q000276:immunology*; D002469:Cell Separation / Q000379:methods; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D016130:Immunophenotyping; D020381:Interleukin-17 / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010920:Placenta / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy
D020381:Interleukin-17