Prognostic relevance of the expressions of CAV1 and TES genes on 7q31 in melanoma.
The 7q31 locus contains several genes affected in cancer progression. Although evidences exist regarding its impact on tumorigenesis, the role of genetic alterations and the expressions of locus-related genes are still controversial. Our study aimed to define the 7q31 copy number alterations in primary melanomas, primary-metastatic tumor pairs and cell lines. Data were correlated with clinical-pathological parameters. Genetic data show that 7q31 copy number distribution was heterogeneous in both primary and metastatic tumors. Extra copies were highly accompanied by chromosome 7 polisomy, and significantly increased in primary lesions with poor prognosis. Additionally, we determined the mRNA and protein levels of the locus-related CAV1 and TES genes. TES mRNA level was associated with metastatic location. CAV1 mRNA and protein levels were significantly higher in thicker tumors, however, lack of protein was also observed in a subpopulation of thin lesions. Expressions of CAV1 and TES were not associated with 7q31 alterations. In conclusion, 7q31 amplification can predict unfavorable outcome. Alterations of TES mRNA level may predict the location of metastasis. CAV1 possibly affect the cancer cell invasion.
Department of Preventive Medicine, Faculty of Public Health, Medical and Health Science Center, University of Debrecen, Hungary.
Laura
L
Vizkeleti
Szilvia
S
Ecsedi
Zsuzsa
Z
Rakosy
Agnes
A
Begany
Gabriella
G
Emri
Reka
R
Toth
Adrienn
A
Orosz
Attila Gabor
AG
Szollosi
Gabor
G
Mehes
Roza
R
Adany
Margit
M
Balazs
D000328:Adult; D051242:Caveolin 1 / Q000235:genetics*; D045744:Cell Line, Tumor; D002897:Chromosomes, Human, Pair 7; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D060588:LIM Domain Proteins / Q000235:genetics*; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
C495540:CAV1 protein, human; D051242:Caveolin 1; D003598:Cytoskeletal Proteins; D060588:LIM Domain Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C429688:TES protein, human