Colony-stimulating factor-1 receptor protein expression is a specific marker for goldfish (Carassius auratus L.) macrophage progenitors and their differentiated cell types.
Signaling through the colony-stimulating factor-1 receptor (CSF-1R) mediates the proliferation, differentiation, and activation of macrophages and their progenitors. In this study we report on the use of an anti-goldfish CSF-1R antibody to specifically recognize a population of CSF-1R positive cells from goldfish tissues. Furthermore, using our previously characterized primary kidney macrophage culture system, we show that CSF-1R positive cells include monocytes, macrophages, and their progenitor cells. Freshly isolated progenitor cells had a higher median florescent intensity ratio than those progenitor cells cultured for up to four days. The decrease in CSF-1R expression on the progenitor cells coincides with the appearance and development of monocytes and macrophages. Monocytes were consistently CSF-1R+ and maintained the high level of CSF-1R expression as they developed into macrophages. Like that of mammalian systems, CSF-1R is expressed on all macrophage sub-populations (progenitors, monocytes, macrophages), and CSF-1R expression increases with macrophage development in teleosts.
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Barbara A
BA
Katzenback
Miodrag
M
Belosevic
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006054:Goldfish / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D016186:Receptor, Macrophage Colony-Stimulating Factor; D013234:Stem Cells / Q000378:metabolism
D015415:Biomarkers; D016186:Receptor, Macrophage Colony-Stimulating Factor