Colloidal gold-based immunochromatographic test strip for rapid detection of abrin in food samples.
In the present study, we developed a convenient, rapid, and sensitive immunochromatographic (IC) test strip to detect abrin in assay buffer and spiked abrin in test food samples. The abrin IC test strip was based on a sandwich format consisting of a monoclonal antibody and a polyclonal  antibody. The anti-abrin A chain monoclonal antibody from mice was immobilized on a porous nitrocellulose membrane as a capture antibody, while the anti-abrin polyclonal antibody from rabbits was conjugated to colloidal gold particles, serving as a detection antibody. Both visual observation  and quantitative analysis indicated that the lower detection of the strip was about 3 ng/ml when abrin was directly spiked into milk, orange juice, and drinking water at a concentration of 3 to 60 ng/ml; the analytical recovery rate was 92.2 to 128%. With this method, abrin spiked into food  could be detected in less than 10 min. Moreover, the IC test strip showed no cross-reaction with the closely related phytotoxin ricin. Therefore, our test strip is an ideal candidate for the development of a kit for rapid and quantitative detection of abrin in food samples.
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing 100071, People's Republic of China.
Shan
S
Gao
Chong
C
Nie
Junhong
J
Wang
Jing
J
Wang
Lin
L
Kang
Yang
Y
Zhou
Jing-Lin
JL
Wang
D000036:Abrin / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D051379:Mice; D011817:Rabbits; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D000036:Abrin