[Proteomics analysis of feather pulp from chickens infected with very virulent strain of Marek's disease virus].
OBJECTIVE
Feather follicle epithelium (FFE) and feather of chicken are sites that produce and release enveloped infectious Marek's disease virus (MDV). In order to investigate host responses, the feather pulp from chickens infected with MDV was analyzed by proteomics.


METHODS
Forty-eight one-day old specific pathogen free (SPF) chickens were randomly divided into two groups. One group of birds (n = 24) were inoculated intraperitoneally with 1000 plaque-forming unit (PFU) of the RB1B strain of MDV, the rest (n = 24) kept as uninfected control. Feather pulp were extracted from feather tips collected from chickens of infected and control group at 21 days post infected (dpi), and dissolved in two dimensional electrophoresis (2-DE) sample buffer. The soluble proteins were separated by 2-DE, 6 images (2 groups x 3 images) of 2-DE were used to analyze the differentially expressed proteins with PDQuest 8. 0. 1. Some of spots changed significantly were further analyzed by mass spectrometry.


RESULTS
41 spots, which expression level changed above two fold, were detected. Among of them, 25 of these spots were up-regulated, 7 spots down-regulated, 9 spots newly induced expression in group infected with MDV. 21 spots, corresponding to 20 proteins, were successfully identified by mass spectrometry. These differential expressed proteins are apolipoprotein A I, 14-3-3 sigma (two spots are the same protein), stathmin, and so on.


CONCLUSIONS
Bioinformatics analysis indicates these differential proteins are mainly associated with host response, metabolism, cytoskeleton, and cell proliferation.
Key Laboratory of Preventive Veterinary Medicine of Jiangsu, School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. yixhchen@163.com
Xinhong
X
Chen
Zhanjun
Z
Lu
Kun
K
Qian
Wenjie
W
Jin
You
Y
Wang
Aijian
A
Qin
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005241:Feathers / Q000737:chemistry* / Q000378:metabolism / Q000821:virology; D008381:Herpesvirus 2, Gallid / Q000472:pathogenicity* / Q000502:physiology; D008380:Marek Disease / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
D011506:Proteins