The effect of adenosine on pro-inflammatory cytokine production by porcine T cells.
Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-α, IFN-γ, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-α and Th1-associated cytokines IFN-γ, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations.
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Petra
P
Ondrackova
Hana
H
Kovaru
Frantisek
F
Kovaru
Jan
J
Matiasovic
Lenka
L
Leva
Martin
M
Faldyna
D000241:Adenosine / Q000819:agonists* / Q000502:physiology; D019830:Adenosine-5'-(N-ethylcarboxamide) / Q000494:pharmacology*; D000818:Animals; D017252:CD3 Complex / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000662:veterinary; D007249:Inflammation / Q000503:physiopathology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000502:physiology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000502:physiology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology; D011799:Quinazolines / Q000494:pharmacology; D013552:Swine / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D014230:Triazoles / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000502:physiology
D017252:CD3 Complex; D007376:Interleukin-2; D011799:Quinazolines; D014230:Triazoles; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D016753:Interleukin-10; D019830:Adenosine-5'-(N-ethylcarboxamide); D007371:Interferon-gamma; D000241:Adenosine; C052410:9-chloro-2-(2-furyl)-(1,2,4)triazolo(1,5-c)quinazolin-5-imine