A quartz nanopillar hemocytometer for high-yield separation and counting of CD4(+) T lymphocytes.
We report the development of a novel quartz nanopillar (QNP) array cell separation system capable of selectively capturing and isolating a single cell population including primary CD4(+) T lymphocytes from the whole pool of splenocytes. Integrated with a photolithographically patterned hemocytometer structure, the streptavidin (STR)-functionalized-QNP (STR-QNP) arrays allow for direct quantitation of captured cells using high content imaging. This technology exhibits an excellent separation yield (efficiency) of ~95.3 ± 1.1% for the CD4(+) T lymphocytes from the mouse splenocyte suspensions and good linear response for quantitating captured CD4(+) T-lymphoblasts, which is comparable to flow cytometry and outperforms any non-nanostructured surface capture techniques, i.e. cell panning. This nanopillar hemocytometer represents a simple, yet efficient cell capture and counting technology and may find immediate applications for diagnosis and immune monitoring in the point-of-care setting.
Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju, 561-756, Korea.
Dong-Joo
DJ
Kim
Jin-Kyeong
JK
Seol
Yu
Y
Wu
Seungmuk
S
Ji
Gil-Sung
GS
Kim
Jung-Hwan
JH
Hyung
Seung-Yong
SY
Lee
Hyuneui
H
Lim
Rong
R
Fan
Sang-Kwon
SK
Lee
D000818:Animals; D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006434:Hemocytes / Q000166:cytology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological; D049329:Nanostructures / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry*
D011791:Quartz