Simultaneous on-chip DC dielectrophoretic cell separation and quantitative separation performance characterization.
Through integration of a MOSFET-based microfluidic Coulter counter with a dc-dielectrophoretic cell sorter, we demonstrate simultaneous on-chip cell separation and sizing with three different samples including 1) binary mixtures of polystyrene beads, 2) yeast cells of continuous size distribution, and 3) mixtures of 4T1 tumor cells and murine bone marrow cells. For cells with continuous size distribution, it is found that the receiver operator characteristic analysis is an ideal method to characterize the separation performance. The characterization results indicate that dc-DEP separation performance degrades as the sorting throughput (cell sorting rate) increases, which provides insights into the design and operation of size-based microfluidic cell separation.
Department of Mechanical Engineering, Vanderbilt University, Nashville, Tennessee 37235-1592, USA.
Jiashu
J
Sun
Yandong
Y
Gao
Richard J
RJ
Isaacs
Kimberly C
KC
Boelte
P Charles
PC
Lin
Erik M
EM
Boczko
Deyu
D
Li
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D005260:Female; D005434:Flow Cytometry; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry*; D051379:Mice; D046210:Microfluidic Analytical Techniques; D008863:Microspheres; D020411:Oligonucleotide Array Sequence Analysis; D011137:Polystyrenes / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D011137:Polystyrenes