The role of adjuvant in mediating antigen structure and stability.
The purpose of this study was to probe the fate of a model antigen, a cysteine-free mutant of bacteriophage T4 lysozyme, to the level of fine structural detail, as a consequence of its interaction with an aluminum (Al)-containing adjuvant. Fluorescence spectroscopy and differential scanning calorimetry were used to compare the thermal stability of the protein in solution versus adsorbed onto an Al-containing adjuvant. Differences in accessible hydrophobic surface areas were investigated using an extrinsic fluorescence probe, 8-Anilino-1-naphthalenesulfonic acid (ANS). As has been observed with other model antigens, the apparent thermal stability of the protein decreased following adsorption onto the adjuvant. ANS spectra suggested that adsorption onto the adjuvant caused an increase in exposure of hydrophobic regions of the protein. Electrostatic interactions drove the adsorption, and disruption of these interactions with high ionic strength buffers facilitated the collection of two-dimensional (15) N heteronuclear single quantum coherence nuclear magnetic resonance data of protein released from the adjuvant. Although the altered stability of the adsorbed protein suggested changes to the protein's structure, the fine structure of the desorbed protein was nearly identical to the protein's structure in the adjuvant-free formulation. Thus, the adjuvant-induced changes to the protein that were responsible for the reduced thermal stability were not observed upon desorption.
Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA. latoya.jonesbraun@ucdenver.edu
Latoya Jones
LJ
Braun
Aimee M
AM
Eldridge
Jessica
J
Cummiskey
Kelly K
KK
Arthur
Deborah S
DS
Wuttke
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000327:Adsorption; D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D017122:Bacteriophage T4 / Q000201:enzymology; D002152:Calorimetry, Differential Scanning; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D055550:Protein Stability
C515594:8-anilino-1-naphthalenesulfonic acid; D000276:Adjuvants, Immunologic; D000817:Anilino Naphthalenesulfonates; D000941:Antigens; D009113:Muramidase