[Isolation, purification and composition analysis of water-soluble polysaccharide from Halenia elliptica].
OBJECTIVE
To provide scientific cue for the use of Halenia elliptica.


METHODS
The crude polysaccharide was extracted from Halenia elliptica with hot water and precipitated by ethanol. The crude polysaccharide has been eliminated protein and fractionated by acidic ethanol. Four fractions HM1, HM2, HM3 and HM4 were got respectively.


RESULTS
By using DEAE-Sephadex A-25 gel filtration, HM1 was got. GC analysis indicated that HM1, HM2, HM3, HM4 and HM41 were composed of Rha, Ara, Xyl, Man, Gal and Glc. But their molar ratios were different.


CONCLUSIONS
Polysaccharide HM41 is isolated from Halenia elliptica for the first time.
College of Life and Environmental Science, MINZU University of China, Beijing 100081, China. liuchunlan888@yahoo.com.cn
Chun-Lan
CL
Liu
Yang
Y
Li
Xiao
X
Huang
Gui-Yun
GY
Xi
Xi-Na
XN
A
D002849:Chromatography, Gas / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D029965:Gentianaceae / Q000737:chemistry*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D014961:X-Ray Diffraction
D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D011134:Polysaccharides