A novel extract from bovine colostrum whey supports anti-bacterial and anti-viral innate immune functions in vitro and in vivo: I. Enhanced immune activity in vitro translates to improved microbial clearance in animal infection models.
OBJECTIVE
To evaluate effects on the innate immune system after exposure to, a consumable low-molecular weight fraction (CLMWF) of immunoglobulin-depleted bovine colostrum whey.


METHODS
Cell-based immune assays were performed in vitro, and host resistance towards bacterial and viral infection was evaluated in two mouse studies.


RESULTS
In vitro data showed a multimodal effect, as CLMWF treatment resulted in a rapid increase in phagocytosis. CLMWF increased chemotaxis of polymorphonuclear cells towards the bacterial peptide f-MLP. CLMWF treatment of natural killer cells increased expression of the CD69 activation marker. Mononuclear phagocytes showed decreased numbers of CD14(bright) and increased number of CD14(dim) cells. The remaining CD14(bright) cells showed reduced expression of CD80 and CD86, whereas CD14(dim) cells showed increased expression of CD80 and CD86, suggesting dendritic cell maturation. Mouse models were applied to evaluate the immune-modulating capacity of CLMWF when consumed acutely during bacterial (Streptococcus) and viral (Influenza) infections in vivo. Reduced bacterial and viral loads were observed in lungs within 24h. Viral load was also reduced when CLMWF was introduced intranasally.


CONCLUSIONS
The data suggest that the support of antimicrobial immune defense mechanisms and maturation of antigen-presenting cells in vitro translates to protection in vivo when product is introduced across mucosal membranes.
NIS Labs, Klamath Falls, OR 97601, USA. Kathy@nislabs.com
Kathleen F
KF
Benson
Steve G
SG
Carter
Kelly M
KM
Patterson
Dilip
D
Patel
Gitte S
GS
Jensen
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D004195:Disease Models, Animal; D007113:Immunity, Innate / Q000187:drug effects*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D051379:Mice; D050296:Microbial Viability; D008894:Milk Proteins / Q000494:pharmacology*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D013290:Streptococcal Infections / Q000276:immunology* / Q000382:microbiology; D019562:Viral Load; D000067816:Whey Proteins
D015703:Antigens, CD; D008894:Milk Proteins; D000067816:Whey Proteins