Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells.
Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-α production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy.
Department of Ecology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, Bulevar despota Stefana 142, 11000 Belgrade, Serbia.
Sandra
S
Belij
Aleksandra
A
Popov
Lidija
L
Zolotarevski
Ivana
I
Mirkov
Jelena
J
Djokic
Dragan
D
Kataranovski
Milena
M
Kataranovski
D000279:Administration, Cutaneous; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D007155:Immunologic Factors / Q000008:administration & dosage*; D015850:Interleukin-6 / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004137:Dinitrochlorobenzene; D007155:Immunologic Factors; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D009195:Peroxidase