Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A.
The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression.
The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Jianlong
J
He
Kouta
K
Sakaguchi
Tohru
T
Suzuki
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001644:Bifidobacterium / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D018387:Codon, Initiator / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D018387:Codon, Initiator; D021901:DNA, Intergenic; D012333:RNA, Messenger