The use of magnetic resonance cell tracking to monitor endothelial progenitor cells in a rat hindlimb ischemic model.
A water-soluble magnetic resonance imaging (MRI) contrast agent, Dextran mono-N-succinimidyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate-gadolinium(3+) (Dex-DOTA-Gd(3+)), was shown to enable monitoring of the anatomical migration and the survival period of transplanted stem cells for up to 1 month. Gadolinium molecules in the cells were rapidly eliminated from the site and excreted upon cell death. Endothelial progenitor cells (EPCs) transplanted into the inguinal femoral muscle of rats migrated distally through the knee in rats after hindlimb ischemia but did not migrate in non-ischemic rats. Interestingly, the survival period of transplanted EPCs was notably prolonged in the ischemic limb, indicating that EPCs are required by the ischemic tissues and that the fate of transplanted EPCs was affected by the disease. Compared to the commonly used particle type of MRI contrast agents, the system described in this study is expected to be invaluable to help clarifying the process of stem cell transplantation therapy.
Department of Biomedical Engineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
Carlos A
CA
Agudelo
Yoichi
Y
Tachibana
Andres F
AF
Hurtado
Takayuki
T
Ose
Hidehiro
H
Iida
Tetsuji
T
Yamaoka
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods*; D003911:Dextrans / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005682:Gadolinium / Q000493:pharmacokinetics; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000494:pharmacology; D006614:Hindlimb / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D021621:Imaging, Three-Dimensional; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D012039:Regional Blood Flow / Q000187:drug effects; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
D003911:Dextrans; D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D005682:Gadolinium