Fibroblast growth factor 2 inhibits the expression of stromal cell-derived             factor 1α in periodontal ligament cells derived from human permanent teeth in             vitro.
Although cells derived from periodontal ligament (PDL) tissue are reported to have stem cell-like activity and are speculated to play a crucial role for tissue healing and regeneration after injury or orthodontic treatment, mechanisms regulating their recruitment and activation remain unknown. Recently, stromal cell-derived factor 1α (SDF-1α) has been reported to be important for stem cell homing and recruitment to injured sites. The aim of this study was to evaluate whether fibroblast growth factor 2 (FGF-2) affects the expression of SDF-1α in PDL cells derived from human permanent teeth in vitro. Using real-time PCR, the expression of SDF-1α mRNA in PDL cells was inhibited by treatment with 10 ng/ml FGF-2. When PDL cells were treated with SU5402 (an inhibitor of FGF receptor 1) in combination with FGF-2, the FGF-2-reduced expression of SDF-1α was inhibited. In the presence of the JNK inhibitor SP600125, SDF-1α mRNA in PDL cells was not suppressed by the FGF-2 treatment. Western blot analysis also showed that SDF-1α production was suppressed by treatment with FGF-2, but it recovered with treatment by FGF-2 + SU5402. These findings suggest that SDF-1α from PDL cells plays an important role in the regeneration and homeostasis of periodontal tissues via the recruitment of stem cells.
Department of Pediatric Dentistry, School of Dentistry, Iwate                     Medical University, Morioka 020-8505, Japan.
Takeyoshi
T
Asakawa
Naoyuki
N
Chosa
Yoshitaka
Y
Yoshimura
Asami
A
Asakawa
Mitsuro
M
Tanaka
Akira
A
Ishisaki
Masato
M
Mitome
Tomokazu
T
Hasegawa
D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D013234:Stem Cells; D014070:Tooth / Q000166:cytology
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D011758:Pyrroles; D012333:RNA, Messenger; C105686:SU 5402; D016222:Fibroblast Growth Factor 2; D051496:Receptor, Fibroblast Growth Factor, Type 1