Chemometric evaluation of brompheniramine-tannate complexes.
The objective of the current study was to evaluate the performance of Raman and near-infrared (NIR) techniques combined with chemometrics in characterizing the critical quality attributes of brompheniramine (BP)-tannate complexes. Seven complexes were prepared and evaluated for chemical interactions, solubilities, dissolutions, and spatial distributions by NIR chemical imaging (CI). Principal component analysis (PCA) was applied before either partial least squares regression (PLSR) or principal component regression (PCR) models were developed. Complexation was confirmed by Fourier transform IR analysis to yield complexes of lower drug solubilities and sustained-release characteristics in alkaline media. PCA results showed better discrimination ability by NIR than by Raman spectroscopy. Compared with PCR, the PLSR predictions errors, calculated from the Raman and NIR data with second-derivative pretreatment, showed lesser values of 2.68, 0.37, 1.79, and 5.60 and 0.58, 0.25, 0.93, and 0.58 for complex solubilities in acidic and alkaline media and percentages dissolved after 1 and 20 h, respectively. In addition, good correlation (>0.95) was obtained for predicting the drug concentration using PLSR score images explaining the validity of the NIR-CI model for spatial quantitation of BP within its tannate complexes. In conclusion, the chemometric analysis of NIR and/or Raman spectra represented an innovative approach to determine the tannate complexation variability.
Division of Product Quality and Research, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Ahmed S
AS
Zidan
Ziyaur
Z
Rahman
Mansoor A
MA
Khan
D001977:Brompheniramine / Q000737:chemistry*; D006634:Histamine H1 Antagonists / Q000737:chemistry*; D016018:Least-Squares Analysis; D025341:Principal Component Analysis; D012044:Regression Analysis; D019265:Spectroscopy, Near-Infrared; D013059:Spectrum Analysis, Raman; D013634:Tannins / Q000737:chemistry*
D006634:Histamine H1 Antagonists; D013634:Tannins; D001977:Brompheniramine