Colorimetric tracking of protein structural evolution based on the distance-dependent light scattering of embedded gold nanoparticles.
In this communication, we describe a new, simplified colorimetric method for in situ tracking of structural evolution of Cu/Zn-superoxide dismutase (SOD1) aggregates, based on changes in plasmonic coupling between gold nanoparticles (GNPs) embedded along the structural backbone of the SOD1 aggregates.
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul, 151-742, Korea.
Inhee
I
Choi
Young In
YI
Yang
Eunhye
E
Jeong
Kihoon
K
Kim
Surin
S
Hong
Taewook
T
Kang
Jongheop
J
Yi
D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D012542:Scattering, Radiation; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism; D000072105:Superoxide Dismutase-1
D006046:Gold; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1