Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part II: gene regulation.
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study. Expression profiles of ntESCs established by different NT-methods (piezoelectric microinjection or zona-free) were indistinguishable. However, expression profiling analyses identified differentially regulated genes between reference ESCs and ntESCs from different genetic backgrounds. A number of pluripotency and stemness marker genes significantly differed at the mRNA level between the cell lines. However, cluster and lineage analyses revealed that such differences had no effect on cell differentiation and cell fate. Regardless of the donor cell type, gene expression profiles of ntESCs were more similar to each other than to their counterpart fertilized embryo-derived ESCs of the same genotype. Overall, the results indicated that expression profile differences may be related to the genotype rather than to technical variations.
Genetic Reprogramming Group, Agricultural Biotechnology Center, Gödöllő, Hungary.
Julianna
J
Kobolak
Marion
M
Horsch
Sandra
S
Geissler
Solomon
S
Mamo
Johannes
J
Beckers
Andras
A
Dinnyes
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005838:Genotype; D066298:In Vitro Techniques; D059785:Karyotype; D051379:Mice; D023421:Models, Animal; D005810:Multigene Family; D053652:Nuclear Transfer Techniques; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology