Production of alkali tolerant cellulase free xylanase in high levels by Bacillus pumilus SV-205.
The fermentation conditions were optimized for hyper production of xylanase from Bacillus pumilus SV-205. The bacterium secretes high levels (7382.7±1200 IU/mL) of cellulase-free xylanase using wheat bran led to 21.63 fold increase in activity. A combination of yeast extract and peptone stimulated highest xylanase production (2448.0 IU/mL) as compared to other combinations. The most important characteristic of the enzyme is its high pH stability (100%) over a broad pH range of 6-11 for 24h. Thermostability studies revealed that enzyme retained 65% activity after an incubation of 2h at 60°C. The level of production is remarkable as compared to earlier reports.
Department of Biochemistry, Kurukshetra University, Kurukshetra, India.
Sushil
S
Nagar
Anuradha
A
Mittal
Davender
D
Kumar
Vijay Kumar
VK
Gupta
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D004789:Enzyme Activation; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D013696:Temperature
D002244:Carbon; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen