Patient-derived olfactory mucosa cells but not lung or skin fibroblasts mediate axonal regeneration of retinal ganglion neurons.
Although human olfactory mucosa derived cells (OMC) have been used in animal models and clinical trials with CNS repair purposes, the exact identity of these cells in culture with respect to their tissue of origin is not fully understood and their neuroregenerative capacity in vitro has not yet been demonstrated. In this study we have compared human OMC with human ensheathing glia from olfactory bulb (OB) and human fibroblasts from skin and lung. Our results indicate that these different cultured cell types exhibit considerable overlap of antigenic markers such that it is presently not possible to distinguish them immunocytochemically. However, in rat retinal ganglion neuron coculture assays the axonal regenerative activity of OMC and OB ensheathing glia was dramatically higher than that exhibited by all fibroblast samples, confirming neuroregenerative activity as a unique property shared by cultured cells derived from the human olfactory system.
Department of Molecular Biology, Universidad Autónoma de Madrid, 28049 Cantoblanco, Spain.
Vega
V
García-Escudero
Ana
A
García-Gómez
Elena
E
Langa
María Jesús
MJ
Martín-Bermejo
Rafael
R
Ramírez-Camacho
José Ramón
JR
García-Berrocal
María Teresa
MT
Moreno-Flores
Jesús
J
Avila
Filip
F
Lim
D000818:Animals; D001369:Axons / Q000502:physiology*; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008168:Lung / Q000166:cytology; D009416:Nerve Regeneration / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology; D009830:Olfactory Bulb / Q000166:cytology; D009831:Olfactory Mucosa / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000378:metabolism; D012867:Skin / Q000166:cytology
D015415:Biomarkers