Effects of chicken selenoprotein W on H2O2-induced apoptosis in CHO-K1 cells.
Selenoprotein W (SelW) is expressed in various tissues of many animals and acts as an oxidoreductase in mammals. However, little is known about the role of the SelW in birds. To investigate the role of the chicken SelW on H(2)O(2)-induced apoptosis in CHO-K1 cells, overexpression of a chicken SelW cell lines (CHO-K1/SelW) were constructed. Using acridine orange/ethidium bromide (AO/EB) double staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assays, as well as WST-1 cell viability assay, we monitored the extent of the H(2)O(2)-induced apoptosis and detected the abundance of the caspase-3, caspase-8, and fas mRNA by real-time quantitative reverse transcription PCR (qPCR). We here found that overexpression of SelW cells, compared with the wild-type cells, resulted in a markedly decrease in sensitivity to H(2)O(2)-induced oxidative stress and had a lower apoptotic cell death in AO/EB and TUNEL assays. Cell viability revealed that overexpression of SelW cells had higher cell viability than wild-type cells. qPCR results found that overexpression of SelW cells had a lower levels of caspase-3, caspase-8, and fas mRNA than wild-type cells. Taken together, our findings suggested that SelW could reduce the oxidative damage induced by H(2)O(2) and had an important protective function in against oxidative damage.
Department of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
Yan-Hui
YH
Han
Zi-Wei
ZW
Zhang
Jian
J
Su
Bo
B
Zhang
Shu
S
Li
Shi-Wen
SW
Xu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D016466:CHO Cells; D053148:Caspase 3 / Q000235:genetics; D053181:Caspase 8 / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051151:Selenoprotein W / Q000235:genetics* / Q000378:metabolism; D019014:fas Receptor / Q000235:genetics
D016877:Oxidants; D012333:RNA, Messenger; D051151:Selenoprotein W; D019014:fas Receptor; D006861:Hydrogen Peroxide; D053148:Caspase 3; D053181:Caspase 8