Variable expression of human Beta defensins 3 and 9 at the human ocular surface in infectious keratitis.
OBJECTIVE
The authors have previously reported the presence of the antimicrobial peptides human beta defensin (hBD) 3 and hBD9 on the ocular surface (OS). These play an important role in infection and inflammation. In the present study, the authors studied the gene expression levels of hBD3 and hBD9 in healthy subjects and during and after healing of infectious keratitis.


METHODS
Human OS specimens were obtained by impression cytology from healthy controls and patients with Acanthamoeba and Gram-negative and -positive bacterial keratitis (BK), both during active infection and after healing. The gene expression levels of hBD3 and hBD9 were determined using quantitative real-time polymerase chain reaction (RT-PCR).


RESULTS
hBD3 and hBD9 were constitutively expressed in all healthy controls. During acute Acanthamoeba keratitis (AK), hBD3 levels were markedly increased and then returned close to normal levels after healing. In BK, hBD3 gene expression was moderately increased and then decreased after healing. In contrast to hBD3, hBD9 was significantly downregulated in both AK and Gram-positive BK, whereas it showed an insignificant decrease in Gram-negative BK. After healing, the expression showed upregulation except in Gram-positive BK, where it continued to decline.


CONCLUSIONS
This is the first study that demonstrates the gene expression of hBD3 and hBD9 in response to infection. It illustrates that not all antimicrobial peptides (AMPs) behave in a similar manner. Some are upregulated and some are downregulated, suggesting a diverse role of AMP in infection and inflammation. The results point to a role of AMP-mediated host defense in Acanthamoeba keratitis as well.
Division of Ophthalmology and Visual Sciences, The University of Nottingham, Nottingham, United Kingdom.
Ahmad Muneer
AM
Otri
Imran
I
Mohammed
Mouhamed A
MA
Al-Aqaba
Usama
U
Fares
Chen
C
Peng
Andrew
A
Hopkinson
Harminder S
HS
Dua
D023181:Antimicrobial Cationic Peptides; D003315:Cornea / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D003937:Diagnosis, Differential; D015818:Eye Infections, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007634:Keratitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D023083:beta-Defensins / Q000096:biosynthesis / Q000235:genetics*
D023181:Antimicrobial Cationic Peptides; C526629:DEFB-109 peptide, human; C422950:DEFB103A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins