Two G-protein-coupled-receptor candidates, Cand2 and Cand7, are involved in Arabidopsis root growth mediated by the bacterial quorum-sensing signals N-acyl-homoserine lactones.
Many Gram-negative bacteria use N-acyl-homoserine lactones (AHLs) as quorum sensing (QS) signaling molecules to coordinate their group behavior. Recently, it was shown that plants can perceive and respond to these bacterial AHLs. However, little is known about the molecular mechanism underlying the response of plants to bacterial QS signals. In this study, we show that the promotion of root elongation in wild type Arabidopsis thaliana induced by the AHLs N-3-oxo-hexanoyl-homoserine lactone (3OC6-HSL) or N-3-oxo-octanoyl-homoserine lactone (3OC8-HSL) was completely abolished in plants with loss-of-function mutations in two candidate G-protein Coupled Receptors (GPCRs), Cand2 and Cand7. Furthermore, real-time PCR analysis revealed that the expression levels of Cand2 and Cand7 were elevated in plants treated with 3OC6-HSL or 3OC8-HSL. These results suggest that Cand2 and Cand7 are involved in the regulation of root growth by bacterial AHLs and that GPCRs play a role in mediating interactions between plants and microbes.
Biology Institute, Hebei Academy of Sciences, Shijiazhuang, China. jinguopingapple@163.com
Guoping
G
Jin
Fang
F
Liu
Hong
H
Ma
Shaoyan
S
Hao
Qian
Q
Zhao
Zirui
Z
Bian
Zhenhua
Z
Jia
Shuishan
S
Song
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology; D053038:Quorum Sensing; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; C571039:Cand2 protein, Arabidopsis; C571040:Cand7 protein, Arabidopsis; C511789:N-(3-oxooctanoyl)homoserine lactone; D043562:Receptors, G-Protein-Coupled; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D015107:4-Butyrolactone