Impaired release of antimicrobial peptides into nasal fluid of hyper-IgE and CVID patients.
BACKGROUND
Patients with primary immunodeficiency (PID) often suffer from frequent respiratory tract infections. Despite standard treatment with IgG-substitution and antibiotics many patients do not improve significantly. Therefore, we hypothesized that additional immune deficits may be present among these patients.


OBJECTIVE
To investigate if PID patients exhibit impaired production of antimicrobial peptides (AMPs) in nasal fluid and a possible link between AMP-expression and Th17-cells.


METHODS
Nasal fluid, nasopharyngeal swabs and peripheral blood mononuclear cells (PBMCs) were collected from patients and healthy controls. AMP levels were measured in nasal fluid by Western blotting. Nasal swabs were cultured for bacteria. PBMCs were stimulated with antigen and the supernatants were assessed for IL-17A release by ELISA.


RESULTS
In healthy controls and most patients, AMP levels in nasal fluid were increased in response to pathogenic bacteria. However, this increase was absent in patients with common variable immunodeficiency (CVID) and Hyper-IgE syndrome (HIES), despite the presence of pathogenic bacteria. Furthermore, stimulation of PBMCs revealed that both HIES and CVID patients exhibited an impaired production of IL-17A.


CONCLUSIONS
CVID and HIES patients appear to have a dysregulated AMP response to pathogenic bacteria in the upper respiratory tract, which could be linked to an aberrant Th17 cell response.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Andreas
A
Cederlund
Marie
M
Olliver
Rokeya Sultana
RS
Rekha
Monica
M
Lindh
Lennart
L
Lindbom
Staffan
S
Normark
Birgitta
B
Henriques-Normark
Jan
J
Andersson
Birgitta
B
Agerberth
Peter
P
Bergman
D000890:Anti-Infective Agents / Q000378:metabolism*; D015153:Blotting, Western; D001826:Body Fluids / Q000378:metabolism; D016022:Case-Control Studies; D017074:Common Variable Immunodeficiency / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007589:Job Syndrome / Q000378:metabolism*; D009296:Nasal Cavity / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
D000890:Anti-Infective Agents; D010455:Peptides