In vitro antitumour activity of stearic acid-g-chitosan oligosaccharide polymeric micelles loading podophyllotoxin.
Development of successful formulations for poorly water-soluble drugs remains a longstanding critical and challenging issue in cancer therapy. The stearic acid-g-chitosan oligosaccharide (CSO-SA) micelles have been presented as potential candidates for intracellular antitumour agent delivery carrier. Herein, podophyllotoxin (PPT) loaded CSO-SA micelles (CSO-SA/PPT) were prepared by a dialysis method. The drug encapsulation efficiency could reach a higher level, the micellar size and the zeta potential increased with increasing charged amounts of drug. The cumulative release percentage of PPT drug from micelles enhanced with decreasing PPT content in the micelles. The cytotoxicities of CSO-SA/PPT micelles against human breast carcinoma (MCF-7) cells, human lung cancer cells (A549) and human hepatoma cell line (Bel-7402) were higher than that of free PPT formulation. The higher cytotoxicities were due to the faster PPT transport into tumour cells mediated by CSO-SA micelles. Overall, CSO-SA micelles might be a promising carrier for PPT delivery in cancer therapy.
Department of Pharmacy, School of Medicine Science, Jiaxing University, Jiaxing, Zhejiang, China.
Xuan
X
Huang
Xiong
X
Huang
Xiao-Hong
XH
Jiang
Fu-Qiang
FQ
Hu
Yong-Zhong
YZ
Du
Qi-Feng
QF
Zhu
Cheng-Sheng
CS
Jin
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008823:Micelles; D009844:Oligosaccharides / Q000737:chemistry*; D011034:Podophyllotoxin / Q000008:administration & dosage*; D013229:Stearic Acids / Q000737:chemistry*
D000970:Antineoplastic Agents; D008823:Micelles; D009844:Oligosaccharides; D013229:Stearic Acids; D048271:Chitosan; D011034:Podophyllotoxin