[Genetic analysis and gene mapping of DDF1, a pleiotropic gene involving in both vegetable and reproductive growth in rice].
There are many pleiotropic genes playing key roles in regulating both vegetative growth and reproductive development in plants. A dwarf mutant of rice with deformed flowers, named as ddf1, was identified from indica rice breeding lines. Genetic analysis indicated that ddf1 was resulted from the recessive mutation of a single gene, temporarily named as DDF1. This result suggested that DDF1 is a pleiotropic gene, which controls both vegetative growth and reproductive development in rice. To map this gene, an F2 population was developed by crossing the ddf1 heterozygote with the tropical japonica rice variety DZ60. By means of bulked segregant analysis and small population-based linkage analysis using the published RM-series rice SSR markers, DDF1 was preliminarily mapped in a region between markers RM588 and RM587 on chromosome 6 with the genetic distances of 3.8 cM and 2.4 cM to the two markers, respectively. By developing new SSR markers in this interval according to the published rice genome sequence, we further mapped DDF1 in a 165 kb interval. The results will facilitate cloning of DDF1.
Fujian Agricultural < Forestry University, Fuzhou, China. Lishun1981@126.com
Sheng-Ping
SP
Li
Yuan-Lin
YL
Duan
Zhi-Wei
ZW
Chen
Hua-Zhong
HZ
Guan
Chuan-Lei
CL
Wang
Lei-Lei
LL
Zheng
Yuan-Chang
YC
Zhou
Wei-Ren
WR
Wu
D002874:Chromosome Mapping; D017343:Genes, Plant; D058685:Genetic Pleiotropy; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development