Regulation of xylanase biosynthesis in Bacillus cereus BSA1.
Microbial xylanases have a promising biotechnological potential to be used in industries. In this study, regulation of xylanase production was examined in Bacillus cereus BSA1. Xylanase production was induced by xylan. The enzyme production further increased in the presence of xylose and arabinose in very low concentration with addition of xylan (0.5% up to 6.02 U/ml). Addition of glucose (about 0.1%) to the media supplemented with xylan repressed xylanase production. Even higher concentration (>0.1%) of xylose and arabinose repressed xylanase biosynthesis. Glucose-mediated repression was partially relived by addition of cyclic adenosine monophosphate. Chemical like 2-4-dinitrophenol, which can inhibit adenosine triphosphate synthesis in cell, repressed xylanase synthesis and it suggested xylanase synthesis to be an energy dependent process.
Post Graduate Department of Botany, Ramananda College, Bishnupur, Bankura 722122, West Bengal, India.
Asish
A
Mandal
Sanjay
S
Kar
Pradeep Kumar
PK
Das Mohapatra
Chiranjit
C
Maity
Bikas Ranjan
BR
Pati
Keshab Chandra
KC
Mondal
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D009711:Nucleotides / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D014990:Xylans / Q000378:metabolism
D009711:Nucleotides; D014990:Xylans; D002244:Carbon; D000242:Cyclic AMP; D043364:Endo-1,4-beta Xylanases; D019297:2,4-Dinitrophenol