The association of human primary bone cells with biphasic calcium phosphate (βTCP/HA 70:30) granules increases bone repair.
This work evaluates the suitability of biphasic calcium phosphate (BCP) granules (β-TCP/HA 70:30) as potential carriers for cell-guided bone therapy. The BCP granules were obtained by synthesis in the presence of wax, thermal treatment, crushing and sieving and characterized by scanning electron microscopy (SEM), X-ray diffraction and Fourier transform infrared spectroscopy. The cytocompatibility of the BCP granules was confirmed by a multiparametric cytotoxicity assay. SEM analysis showed human bone cell adhesion and migration after seeding onto the material. Rat subcutaneous xenogeneic grafting of granules associated to human bone cells revealed a more accentuated moderate chronic inflammatory infiltrate, without signs of a strong xenoreactivity. Histomorphometrical analysis of bone repair of defects in rat skulls (∅ = 5 mm) has shown that bone cell associated-BCP and autograft promoted a two- and threefold increase, respectively, on new bone formation after 45 days, as compared to BCP alone and blood clot. The increase in bone repair supports the suitability the biocompatible (70:30) BCP granules as injectable and mouldable scaffolds for human cells in bone bioengineering.
Post-Graduation Program on Medical Sciences, Fluminense Federal University (UFF), Niteroi, RJ, Brazil.
Ronaldo de Oliveira
Rde O
Lomelino
Igor Iuco
II
Castro-Silva
Adriana Brandão Ribeiro
AB
Linhares
Gutemberg Gomes
GG
Alves
Sílvia Raquel de Albuquerque
SR
Santos
Vinicius Schott
VS
Gameiro
Alexandre Malta
AM
Rossi
José Mauro
JM
Granjeiro
D016723:Bone Remodeling; D001842:Bone and Bones / Q000166:cytology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D008855:Microscopy, Electron, Scanning
D002130:Calcium Phosphates