Fed-batch anaerobic valorization of slaughterhouse by-products with mesophilic microbial consortia without methane production.
This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhouses, which are becoming available in large amounts as a consequence of the growing constraints for waste disposal from meat industry. A reconstituted microbial mesophilic consortium without Archaebacteria (methanogens), named PBr, was cultivated in a 5-L anaerobic bioreactor on slaughterhouse wastes. The experiments were carried out with sequential fed-batch operations, including liquid medium removal from the bioreactor and addition of fresh substrate. VFAs and nitrogen were the main metabolites observed, while hydrogen accumulation was very low and no methane production was evidenced. After 1,300 h of culture, yields obtained for VFAs reached 0.38 g/g dry matter. Strain composition of the microbial consortium was also characterized using molecular tools (temporal temperature gradient gel electrophoresis and gene sequencing).
Université Blaise Pascal Laboratoire de Génie Chimique et Biochimique, Polytech Clermont-Ferrand, Clermont Université, 24 av. des Landais, BP 206, 63174 Aubière cedex, France.
J
J
Pessiot
R
R
Nouaille
M
M
Jobard
R R
RR
Singhania
A
A
Bournilhas
G
G
Christophe
P
P
Fontanille
P
P
Peyret
G
G
Fonty
C
C
Larroche
D000003:Abattoirs; D000693:Anaerobiosis; D001483:Base Sequence; D019149:Bioreactors; D003470:Culture Media; D017931:DNA Primers; D008697:Methane / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D003470:Culture Media; D017931:DNA Primers; D008697:Methane