A novel function of the human chaperonin CCT epsilon subunit in yeast.
A malfunction in the yeast HAC1 causes the unfolding-protein response in the endoplasmic reticulum, resulting in stress-sensitive and inositol auxotrophic phenotypes. Chaperonin-containing TCP1 (CCT) is necessary for the folding of actin and tubulin in the cytosol. The introduction of the truncated human CCT epsilon subunit into yeast cells of which hac1 was disrupted clearly suppressed not only its inositol auxotrophic phenotype but also its stress-sensitive phenotype.
Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan. nikawa@bio.kyutech.ac.jp
Jun-ichi
J
Nikawa
Minami
M
Kimura
D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D056404:Chaperonin Containing TCP-1 / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018014:Gene Transfer Techniques; D006801:Humans; D007294:Inositol / Q000378:metabolism; D007296:Myo-Inositol-1-Phosphate Synthase / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D056811:Unfolded Protein Response
D050976:Basic-Leucine Zipper Transcription Factors; C091341:HAC1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D007294:Inositol; D056404:Chaperonin Containing TCP-1; C519641:INO1 protein, S cerevisiae; D007296:Myo-Inositol-1-Phosphate Synthase