Evaluation of the presence of microorganisms in solid-organ preservation solution.
OBJECTIVE
To assess the presence of microorganism contamination in the preservation solution for transplant organs (kidney/pancreas).


METHODS
Between August 2007 and March 2008, 136 samples of preservation solution were studied prior to graft implantation. Variables related to the donor and to the presence of microorganisms in the preservation solution of organs were evaluated, after which the contamination was evaluated in relation to the "recipient culture" variable. Univariate and multivariate statistical analyses were performed.


RESULTS
The contamination rate of the preservation solution was 27.9%. Coagulase-negative Staphylococcus was the most frequently isolated microorganism. However, highly virulent agents, such as fungi and enterobacteria, were also isolated. In univariate analysis, the variable "donor antibiotic use" was significantly associated to the contamination of the preservation solution. On the other hand, multivariate analysis found statistical significance in "donor antibiotic use" and "donor's infectious complications" variables.


CONCLUSIONS
In this study, 27.9% of the preservation solutions of transplant organs were contaminated. Infectious diseases and non-use of antibiotics by the donor were significantly related to the presence of microorganisms in organ preservation solutions. Contamination in organ preservation solutions was not associated with infection in the recipient.
Surgical Gastroenterology Service, Universidade Federal de São Paulo-Escola Paulista de Medicina, São Paulo, Brazil.
André Marcelo Colvara
AM
Mattana
Alexandre Rodrigues
AR
Marra
Antônia Maria de Oliveira
AM
Machado
Gaspar de Jesus
Gde J
Lopes Filho
Alcides Augusto
AA
Salzedas Netto
Adriano Miziara
AM
Gonzalez
D004340:Drug Contamination; D005658:Fungi / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D006801:Humans; D016030:Kidney Transplantation; D019188:Organ Preservation Solutions; D016035:Pancreas Transplantation; D011446:Prospective Studies
D019188:Organ Preservation Solutions