Isolation and characterization of high affinity aptamers against DNA polymerase iota.
Human DNA-polymerase iota (Pol ι) is an extremely error-prone enzyme and the fidelity depends on the sequence context of the template. Using the in vitro systematic evolution of ligands by exponential enrichment (SELEX) procedure, we obtained an oligoribonucleotide with a high affinity to human Pol ι, named aptamer IKL5. We determined its dissociation constant with homogenous preparation of Pol ι and predicted its putative secondary structure. The aptamer IKL5 specifically inhibits DNA-polymerase activity of the purified enzyme Pol ι, but did not inhibit the DNA-polymerase activities of human DNA polymerases beta and kappa. IKL5 suppressed the error-prone DNA-polymerase activity of Pol ι also in cellular extracts of the tumor cell line SKOV-3. The aptamer IKL5 is useful for studies of the biological role of Pol ι and as a potential drug to suppress the increase of the activity of this enzyme in malignant cells.
Institute of Molecular Genetics of the Russian Academy of Sciences, 2 Kurchatov Square,Moscow, Russia. lahin9@mail.ru
Andrei V
AV
Lakhin
Andrei A
AA
Kazakov
Alena V
AV
Makarova
Yuri I
YI
Pavlov
Anna S
AS
Efremova
Stanislav I
SI
Shram
Viacheslav Z
VZ
Tarantul
Leonid V
LV
Gening
D052157:Aptamers, Nucleotide / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000276:immunology*; D006801:Humans; D052156:SELEX Aptamer Technique; D000094769:DNA Polymerase iota
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase; D000094769:DNA Polymerase iota; C000722070:POLI protein, human