Optical sensors based on whispering gallery modes in fluorescent microbeads: response to specific interactions.
Whispering gallery modes (WGMs) in surface-fixated fluorescent polystyrene microbeads are studied in view of their capability of sensing the formation of biochemical adsorption layers on their outer surface with the well-established biotin-streptavidin specific binding as the model system. Three different methods for analysis of the observed shifts in the WGM wavelength positions are applied and used to quantify the adsorbed mass densities, which are then compared with the results of a comparative surface plasmon resonance (SPR) study.
Fujirebio, Inc., 51 Komiya-cho, Hachioji-shi 192-0031, Tokyo, Japan. m.h@nanobioanalytics.com
Michael
M
Himmelhaus
Sivashankar
S
Krishnamoorthy
Alexandre
A
Francois
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D008863:Microspheres; D008954:Models, Biological; D008962:Models, Theoretical; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding / Q000502:physiology; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D014173:Transistors, Electronic
D001710:Biotin; D019809:Streptavidin