Cardamonin exerts potent activity against multiple myeloma through blockade of NF-κB pathway in vitro.
NF-κB plays a major role in the pathology of multiple myeloma. Here, we intended to investigate the regulating effect of cardamonin on NF-κB in myeloma cells. We found for the first time that cardamonin suppressed viability and induced apoptosis of myeloma cells. Cardamonin activated caspase-3 and PARP and suppressed the expression of various anti-apoptotic proteins. We discovered that NF-κB was repressed by cardamonin through suppression of IKK expression and IκBα phosphorylation. Furthermore, the expression of NF-κB-regulated gene products ICAM-1, COX-2 and VEGF was down-regulated by cardamonin. These results suggest that cardamonin blocks NF-κB pathway in human multiple myeloma cells.
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
You
Y
Qin
Chun-Yan
CY
Sun
Fu-Rong
FR
Lu
Xiang-Rong
XR
Shu
Di
D
Yang
Lei
L
Chen
Xiao-Mei
XM
She
Nicholas Miller
NM
Gregg
Tao
T
Guo
Yu
Y
Hu
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D009101:Multiple Myeloma / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
D000970:Antineoplastic Agents; D047188:Chalcones; D016328:NF-kappa B; C436747:cardamonin