Chinese herb mix Tiáo-Gēng-Tāng possesses antiaging and antioxidative effects and upregulates expression of estrogen receptors alpha and beta in ovariectomized rats.
BACKGROUND
Herb mixtures are widely used as an alternative to hormonal therapy in China for treatment of the menopausal syndrome. However, composition of these herb mixtures are complex and their working mechanism is often unknown. This study investigated the effect of Tiáo-Gēng-Tāng (TG-decoction), a Chinese herbal mixture extract, in balancing female hormones, regulating expression of estrogen receptors (ERs), and preventing aging-related tissue damage.


METHODS
Ovariectomized 5-month-old female rats were used to model menopause and treated with either TG-decoction or conjugated estrogen for 8 weeks. Estradiol (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured in serum and in the hypothalamus. Hypothalamic expression of estrogen receptor (ER) alpha and beta were studied by real-time PCR and western blotting. Total antioxidant capacity (T-AOC), oxidation indicator superoxide dismutase (SOD) activity and tissue damage parameter malondialdehyde (MDA) were measured using standard assays. Aging-related ultrastructural alterations in mitochondria were studied in all animals by transmission electron microscopy.


RESULTS
TG-decoction-treatment elevated E2 and lowered FSH in serum of ovariectomized rats. The potency and efficacy of TG-decoction on the hypothalamus was generally weaker than that of conjugated estrogens. However, TG-decoction was superior in upregulating expression of ERα and β. TG-decoction increased hypothalamic SOD and T-AOC levels and decreased MDAlevels and mitochondrial damage in hypothalamic neurons.


CONCLUSIONS
TG-decoction balances female hormones similarly to conjugated estrogens but less effectively. However, it is superior in up regulating ERα and β and exhibits antioxidative antiaging activities. Whilst it shares similar effects with estrogen, TG-decoction also seems to have distinctive and more complex functions and activities.
Gynecology Department, Shanghai Yueyang Integrated Traditioanl Chinese Medicine and Western Medicine Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Gan He Road 110, Shanghai, China. sherryxlw@hotmail.com
Lian-wei
LW
Xu
Lan
L
Kluwe
Ting-ting
TT
Zhang
Sheng-nan
SN
Li
Yan-yan
YY
Mou
Zhen
Z
Sang
Jun
J
Ma
Xiong
X
Lu
Zhuo-jun
ZJ
Sun
D000375:Aging / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008593:Menopause / Q000187:drug effects* / Q000235:genetics; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects*
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta