Rapid, sensitive and label-free detection of Shiga-toxin producing Escherichia coli O157 using carbon nanotube biosensors.
An electronic platform to detect very small amounts of genomic DNA from bacteria without the need for PCR amplification and molecular labeling is described. The system uses carbon nanotube field-effect transistor (FET) arrays whose electrical properties are affected by minute electrical charges localized on their active regions. Two pathogenic strains of E. coli are used to evaluate the detection properties of the transistor arrays. Described herein are the results for detection of synthetic oligomers, unpurified and highly purified genomic DNA at various concentrations and their comparison against non-specific binding. In particular, the capture of genomic DNA of E. coli O157:H7 by a specific oligonucleotide probe coated onto the transistor array results in a significant shift in the threshold (gate-source) voltage (V(th)). By contrast the signal under the same procedure using a different strain, E. coli O45 that is non-complementary to the probe remained nearly constant. This work highlights the detection sensitivity and efficacy of this biosensor without stringent requirement for DNA sample preparation.
University of Maryland, College Park, MD 20742, United States.
Sowmya
S
Subramanian
Konrad H
KH
Aschenbach
Jennifer P
JP
Evangelista
Mohamed Badaoui
MB
Najjar
Wenxia
W
Song
Romel D
RD
Gomez
D001483:Base Sequence; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004867:Equipment Design; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013997:Time Factors; D014173:Transistors, Electronic
D004269:DNA, Bacterial; D037742:Nanotubes, Carbon