In vitro antiprotozoal activity of extracts of five Turkish Lamiaceae species.
The in vitro antiprotozoal activities of crude methanolic extracts from the aerial parts of five Lamiaceae plants (Salvia tomentosa, S. sclarea, S. dichroantha, Nepeta nuda subsp. nuda and Marrubium astracanicum subsp. macrodon) were evaluated against four parasitic protozoa, i.e. Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani and Plasmodium falciparum. The cytotoxic potentials of the extracts on L6 cells were also evaluated. Melarsoprol, benznidazole, miltefosine, chloroquine and podophyllotoxin were used as reference drugs. All crude MeOH extracts showed antiprotozoal potential against at least three parasites, so they were dispersed in water and partitioned against n-hexane and chloroform to yield three subextracts that were screened in the same test systems. The n-hexane extract of N. nuda was the most active against T. brucei rhodesiense while the CHCl3 extracts of S. tomentosa and S. dichroantha showed significant activity against L. donovani. All organic extracts displayed in vitro antimalarial and moderate trypanocidal activities against T. cruzi with the n-hexane extract of S. sclarea being the most active against the latter. The extracts displayed low or no cytotoxicity towards mammalian L6 cells.
Department of Pharmacognosy, Faculty of Pharmacy, University of Yeditepe, 34755 Kayisdagi, Istanbul, Turkey.
Hasan
H
Kirmizibekmez
Irem
I
Atay
Marcel
M
Kaiser
Erdem
E
Yesilada
Deniz
D
Tasdemir
D000818:Animals; D000981:Antiprotozoal Agents / Q000032:analysis* / Q000633:toxicity; D002460:Cell Line; D019686:Lamiaceae / Q000737:chemistry* / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D032448:Myoblasts, Skeletal / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D051381:Rats; D014421:Turkey
D000981:Antiprotozoal Agents; D010936:Plant Extracts