Cancer cell detection in tissue sections using AFM.
Currently, cancer diagnosis relies mostly on morphological examination of exfoliated, aspirated cells or surgically removed tissue. As long as standard diagnosis is concerned, this classical approach seems to be satisfactory. In the recent years, cancer progression has been shown to be accompanied by alterations in mechanical properties of cells. This offers the detection of otherwise unnoticed cancer cell disregarded by histological analysis due to insignificant manifestations. One of techniques, sensitive to changes in mechanical properties, is the atomic force microscopy, which detects cancer cells through their elastic properties. Such measurements were applied to tissue sections collected from patients suffering from various cancers. Despite of heterogeneity and complexity of cancer cell sections, the use of the Young's modulus as an indicator of cell elasticity allow for detection of cancer cells in tissue slices.
The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Kraków, Poland. Malgorzata.Lekka@ifj.edu.pl
Małgorzata
M
Lekka
Dorota
D
Gil
Katarzyna
K
Pogoda
Joanna
J
Dulińska-Litewka
Robert
R
Jach
Justyna
J
Gostek
Olesya
O
Klymenko
Szymon
S
Prauzner-Bechcicki
Zbigniew
Z
Stachura
Joanna
J
Wiltowska-Zuber
Krzysztof
K
Okoń
Piotr
P
Laidler
D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D004548:Elasticity; D005260:Female; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*