Isolation of compound microsatellite markers in Begonia fenicis (Begoniaceae) endemic to East and Southeast Asian islands.
OBJECTIVE
We developed compound microsatellite markers for Begonia fenicis, a species endemic to eastern and southeastern Asian islands, to investigate geographical genetic structure.


RESULTS
Using the compound microsatellite marker technique, 21 markers were developed and six polymorphic markers were characterized for samples from four islands in Taiwan and southern Japan. The number of alleles per locus ranged from two to six (mean = 4.33). Observed and expected heterozygosities were 0.125-0.725 (0.383) and 0.498-0.719 (0.641), and polymorphic information content was 0.371-0.664 (0.567). The interspecific transferability of the 21 markers was evaluated for eight species of the section Diploclinium from the Philippines; 15 markers were successfully amplified in one to eight species.


CONCLUSIONS
These results indicate the utility of the six microsatellite markers in B. fenicis to investigate geographical genetic structure. The transferable markers are potentially useful for other species of the section.
Biodiversity Research Center, Academia Sinica, Nangang, Taipei, Taiwan. kohnakamur@gmail.com
Koh
K
Nakamura
Chiun-Jr
CJ
Huang
Rosario Rivera
RR
Rubite
Wai-Chao
WC
Leong
Yoshiko
Y
Kono
Hsun-An
HA
Yang
Ching-I
CI
Peng
D000483:Alleles; D001483:Base Sequence; D031112:Begoniaceae / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers; D005843:Geography; D006579:Heterozygote; D062312:Islands; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013624:Taiwan
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers