Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.
A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.
Hospices Civils de Lyon, Lyon, France.
Sami
S
Slimani
Audrey
A
Robyns
Sophie
S
Jarraud
Maëlle
M
Molmeret
Eric
E
Dusserre
Céline
C
Mazure
Jean Pierre
JP
Facon
Gérard
G
Lina
Jerome
J
Etienne
Christophe
C
Ginevra
D001386:Azides / Q000737:chemistry*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D007875:Legionella / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D008567:Membranes, Artificial; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods*; D011419:Propidium / Q000031:analogs & derivatives* / Q000737:chemistry
D001386:Azides; D004269:DNA, Bacterial; D008567:Membranes, Artificial; C533957:propidium monoazide; D011419:Propidium