Determination of astragalin and astragaloside content in Radix Astragali using high-performance liquid chromatography coupled with pulsed amperometric detection.
Astragalin and astragalosides were measured in Radix Astragali using reversed-phase chromatography coupled with pulsed amperometric detection. Because the target compounds showed poor stability in aqueous solutions, they were extracted in 100% methanol under reflux. All compounds were detected with high sensitivity under highly alkaline conditions using sodium hydroxide as a post-column eluent. The limits of detection and quantification of target compounds were 0.02-0.36 μg/mL and 0.06-1.09 μg/mL, respectively, and the linear regression coefficients were 0.9982-1.0000. The intra- and inter-day precisions were <0.92% in retention time and <4.78% in calculated contents. Average recoveries were >91.33%. Astragalin and astragaloside contents between Radix Astragali at different ages and in different parts were successfully determined without sample purification or concentration.
Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Ha-Jeong
HJ
Kwon
Yong-Duk
YD
Park
D027883:Astragalus Plant / Q000737:chemistry; D027885:Astragalus propinquus / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D044949:Kaempferols; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; C027492:Huang Qi; C001579:astragalin