[Increased genomic instability in somatic cells of the progeny of female mice exposed to acute X-radiation in the preconceptional period].
The level of genome instability (GI) was studied in the progeny of female mice exposed in the preconceptional period to radiation doses of 0.5, 1, and 2 Gy in comparison to that in the progeny of the same parent pairs born before irradiation of the females. To assess the level of genome instability, we analyzed polymorphism of DNA fragments from postmitotic (blood and brain) and proliferating (spleen and tail tip) tissues amplified by AP-PCR (PCR amplification with an arbitrary primer). It was found that polymorphism of the spectrum of AP-PCR products, which is a multilocus genetic marker (MGM), in the genome of somatic cells in the progeny of female mice exposed to 2 Gy was higher than in the progeny of male mice exposed to the same doses. In the progenies of female mice born before and after irradiation, tissue-specific variations in the level of DNA polymorphism were detected. The maximum value of this polymorphism (with respect to the frequency of "nonparental bands") was determined for peripheral blood DNA in comparison with the other tissues. Estimations of the MGM polymorphism with the AP-PCR method demonstrate an increased level of genome instability in somatic cells of offsprings from female mice exposed to a single acute dose of X-rays (0.5, 1, and 2 Gy) in the pre-conceptional period. Radiation-induced transgenerational genome instability with an increase in the dose of preconceptional irradiation of female mice was more pronounced in DNA of the postmitotic tissues (blood and brain DNA) than in DNA of the proliferating tissues (spleen and tail tip epithelium).
M G
MG
Lomaeva
G V
GV
Vasil'eva
L A
LA
Fomenko
V N
VN
Antipova
A I
AI
Gaziev
V G
VG
Bezlepkin
D000818:Animals; D004247:DNA / Q000097:blood; D004307:Dose-Response Relationship, Radiation; D005260:Female; D014644:Genetic Variation / Q000528:radiation effects*; D042822:Genomic Instability / Q000235:genetics*; D008297:Male; D018811:Maternal Exposure; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats / Q000235:genetics / Q000528:radiation effects; D018598:Minisatellite Repeats / Q000235:genetics / Q000528:radiation effects; D019105:Random Amplified Polymorphic DNA Technique; D014965:X-Rays / Q000009:adverse effects
D004247:DNA