Immunostimulation of sugar cane extract on neutrophils to Salmonella typhimurium infection in mice.
The aim of this study was to evaluate the immunomodulatory effects of sugar cane extract (SCE) on the biological activities of neutrophils in mice. Six-week-old BALB/c mice were fed 1250 mg/kg of SCE once. The generation, migration and biological functions of neutrophils and the survival rates of the mice in response to Salmonella typhimurium infection were evaluated. The results show that the numbers of both bone marrow cells and neutrophils were significantly increased in response to SCE administration (p < 0.05) compared with controls. The migration, phagocytosis and H₂O₂ generation of neutrophils were all significantly enhanced in SCE-treated mice (p < 0.05). After challenge with S. typhimurium (lethal dose, 50% (LD₅₀), SCE-treated mice had a 19.2% higher survival rate and milder hepatic lesions than the controls. Additionally, fewer invasive bacteria were recovered from the spleens of SCE-treated mice. In conclusion, our results suggest that SCE has a positive regulatory effect on the biological function of mouse neutrophils that may increase host resistance against bacterial infections.
Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, 250 Kuo Kuang Rd, 40227 Taichung, Taiwan, ROC.
Ming-Hua
MH
Chen
Dan-Yuan
DY
Lo
Jiunn-Wang
JW
Liao
Shih-Ling
SL
Hsuan
Maw-Sheng
MS
Chien
Cheng-Chung
CC
Lin
Ter-Hsin
TH
Chen
Wei-Cheng
WC
Lee
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002465:Cell Movement; D006861:Hydrogen Peroxide / Q000378:metabolism; D007958:Leukocyte Count; D008099:Liver / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis; D010936:Plant Extracts / Q000494:pharmacology*; D031786:Saccharum / Q000737:chemistry*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000382:microbiology
D010936:Plant Extracts; D006861:Hydrogen Peroxide