Biodiversity characterization of cellulolytic bacteria present on native Chaco soil by comparison of ribosomal RNA genes.
Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Proteobacteria and Acidobacteria accounted for more than 76% of all sequences. In both enriched samples, members of Proteobacteria were the most frequently represented. The majority of bacterial genera in both enriched samples were identified as Brevundimonas and Caulobacter, but members of Devosia, Sphingomonas, Variovorax, Acidovorax, Pseudomonas, Xanthomonas, Stenotrophomonas, Achromobacter and Delftia were also found. In addition, it was possible to identify cellulolytic taxa such as Acidothermus, Micromonospora, Streptomyces, Paenibacillus and Pseudomonas, which indicates that this ecosystem could be an attractive source for study of novel enzymes for cellulose degradation.
Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Dr. N. Repetto y Los Reseros s/n, 1686 Hurlingham, Provincia de Buenos Aires, Argentina. ptalia@cnia.inta.gov.ar
Paola
P
Talia
Silvana M
SM
Sede
Eleonora
E
Campos
Marcela
M
Rorig
Dario
D
Principi
Daniela
D
Tosto
H Esteban
HE
Hopp
Daniel
D
Grasso
Angel
A
Cataldi
D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D044822:Biodiversity; D002482:Cellulose / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002482:Cellulose