Cellular uptake of highly-functionalized ruthenium(II) tris-bipyridine protein-surface mimetics.
This manuscript describes cell-uptake studies with HEK 293T cells on a series of ruthenium complexes shown previously to act as receptors for protein surface recognition and was motivated by a desire to establish if these receptors represent suitable templates for further elaboration as inhibitors of protein-protein interactions. The results illustrate that large (>3000Da) highly functionalized anionic ruthenium complexes are efficiently transfected via endocytosis to lysosomes with negligible toxicity.
Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, United Kingdom; Institute of Molecular and Cellular Biology, University of Leeds, Woodhouse Lane, Leeds LS2 9JT, United Kingdom.
Susan J
SJ
Turrell
Maria H
MH
Filby
Adrian
A
Whitehouse
Andrew J
AJ
Wilson
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D008247:Lysosomes / Q000187:drug effects; D018716:Molecular Mimicry / Q000187:drug effects*; D015394:Molecular Structure; D011485:Protein Binding / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds