Type I interferon signaling regulates the composition of inflammatory infiltrates upon infection with Listeria monocytogenes.
Type I IFN is key to the immune response to viral pathogens, however its role in bacterial infections is less well understood. Mice lacking the type I IFN receptor (IFNAR-/-) demonstrate enhanced resistance to infection with Listeriamonocytogenes. We have now determined that following infection with Listeria, the composition of innate cells recruited to the peritoneal cavity of IFNAR-/- mice reflects an increase in the frequency of neutrophils and a decrease in monocyte frequency compared to WT controls. These differences in inflammatory infiltrates could not be attributed to distinct bone marrow composition prior to infection or to level of apoptosis. We also observed no differences in neutrophil oxidative burst. However, blocking CXCR2 prevented enhanced neutrophil influx and hampered bacterial clearance. Taken together, these studies highlight a novel mechanism by which type I interferon signaling regulates the immune response to Listeria, through negative regulation of chemokines driving neutrophil recruitment.
Department of Microbiology and Immunology, Wake Forest University, School of Medicine, Winston-Salem, NC 27157, United States.
Kristina L
KL
Brzoza-Lewis
J Jason
JJ
Hoth
Elizabeth M
EM
Hiltbold
D000818:Animals; D005122:Exudates and Transudates / Q000166:cytology / Q000276:immunology; D005260:Female; D007113:Immunity, Innate / Q000276:immunology; D016898:Interferon-alpha / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D009842:Oligopeptides / Q000494:pharmacology; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000276:immunology*; D023063:Receptors, Interleukin-8B / Q000037:antagonists & inhibitors / Q000276:immunology
D016898:Interferon-alpha; D009842:Oligopeptides; D023063:Receptors, Interleukin-8B; C118980:antileukinate; D053633:Receptor, Interferon alpha-beta