Quercetin protects primary human osteoblasts exposed to cigarette smoke through activation of the antioxidative enzymes HO-1 and SOD-1.
Smokers frequently suffer from impaired fracture healing often due to poor bone quality and stability. Cigarette smoking harms bone cells and their homeostasis by increased formation of reactive oxygen species (ROS). The aim of this study was to investigate whether Quercetin, a naturally occurring antioxidant, can protect osteoblasts from the toxic effects of smoking. Human osteoblasts exposed to cigarette smoke medium (CSM) rapidly produced ROS and their viability decreased concentration- and time-dependently. Co-, pre- and postincubation with Quercetin dose-dependently improved their viability. Quercetin increased the expression of the anti-oxidative enzymes heme-oxygenase- (HO-) 1 and superoxide-dismutase- (SOD-) 1. Inhibiting HO-1 activity abolished the protective effect of Quercetin. Our results demonstrate that CSM damages human osteoblasts by accumulation of ROS. Quercetin can diminish this damage by scavenging the radicals and by upregulating the expression of HO-1 and SOD-1. Thus, a dietary supplementation with Quercetin could improve bone matter, stability and even fracture healing in smokers.
Department of Traumatology, MRI, Techincal University of Munich, 80333 Munich, Germany.
Karl F
KF
Braun
Sabrina
S
Ehnert
Thomas
T
Freude
José T
JT
Egaña
Thilo L
TL
Schenck
Arne
A
Buchholz
Andreas
A
Schmitt
Sebastian
S
Siebenlist
Lilianna
L
Schyschka
Markus
M
Neumaier
Ulrich
U
Stöckle
Andreas K
AK
Nussler
D000975:Antioxidants / Q000378:metabolism; D002470:Cell Survival; D003470:Culture Media / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology; D061251:Primary Cell Culture; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012907:Smoking / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D015854:Up-Regulation
D000975:Antioxidants; D003470:Culture Media; D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; C000606290:SOD1 protein, human; D011794:Quercetin; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1