Effects of ubiquitin C-terminal hydrolase L1 deficiency on mouse ova.
Maternal proteins are rapidly degraded by the ubiquitin-proteasome system during oocyte maturation in mice. Ubiquitin C-terminal hydrolase L1 (UCHL1) is highly and specifically expressed in mouse ova and is involved in the polyspermy block. However, the role of UCHL1 in the underlying mechanism of polyspermy block is poorly understood. To address this issue, we performed a comprehensive proteomic analysis to identify maternal proteins that were relevant to the role of UCHL1 in mouse ova using UCHL1-deficient gad. Furthermore, we assessed morphological features in gad mouse ova using transmission electron microscopy. NACHT, LRR, and PYD domain-containing (NALP) family proteins and endoplasmic reticulum (ER) chaperones were identified by proteomic analysis. We also found that the 'maternal antigen that embryos require' (NLRP5 (MATER)) protein level increased significantly in gad mouse ova compared with that in wild-type mice. In an ultrastructural study, gad mouse ova contained less ER in the cortex than in wild-type mice. These results provide new insights into the role of UCHL1 in the mechanism of polyspermy block in mouse ova.
Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Sayaka
S
Koyanagi
Hiroko
H
Hamasaki
Satoshi
S
Sekiguchi
Kenshiro
K
Hara
Yoshiyuki
Y
Ishii
Shigeru
S
Kyuwa
Yasuhiro
Y
Yoshikawa
D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005260:Female; D005306:Fertilization / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D008954:Models, Biological; D009866:Oogenesis / Q000235:genetics* / Q000502:physiology; D010063:Ovum / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D040901:Proteomics; D043222:Ubiquitin Thiolesterase / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D051339:Actin Depolymerizing Factors; D000199:Actins; D043222:Ubiquitin Thiolesterase; C496976:Uchl1 protein, mouse