A polycation-chaperoned in-stem molecular beacon system.
In the presence of poly(L-lysine)-graft-dextran, an in-stem molecular beacon involving three perylene-anthraquinone pairs in the stem region had a signal/background ratio of as high as 570. Response speed was also remarkable; equilibrium was attained within 5 minutes after addition of substrate DNA at 20 °C.
Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan. asanuma@mol.nagoya-u.ac.jp
Hiroyuki
H
Asanuma
Takuya
T
Osawa
Hiromu
H
Kashida
Taiga
T
Fujii
Xingguo
X
Liang
Kosuke
K
Niwa
Yasuko
Y
Yoshida
Naohiko
N
Shimada
Atsushi
A
Maruyama
D000880:Anthraquinones / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D010569:Perylene / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011107:Polylysine / Q000737:chemistry*
D000880:Anthraquinones; D004247:DNA; D015342:DNA Probes; D003911:Dextrans; D005456:Fluorescent Dyes; D010569:Perylene; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine