Hematological changes in case of chronic cadmium intoxication and monensin detoxication. Relationship with rheological variables.
The study evaluated the affect of chronic cadmium (Cd) and monensin treatment on some hematological parameters and its relationship with the rheological variables. Adult male mice were subjected to chronic treatment with cadmium acetate [Cd(CH3COO)2 × 2H2O] (group 1), Cd(CH3COO)2 × 2H2O followed by treatment with low dose monensin (group 2) and Cd(CH3COO)2 × 2H2O followed by high dose monensin treatment (group 3). Cd(CH3COO)2 × 2H2O and deprotonated monensin were dissolved in distilled water and given daily to the experimental animals. Mice drinking distilled water served as a control group (group 4). Hematological parameters and erythrocyte morphology were evaluated in parallel with whole blood viscosity (WBV). Cd treatment reduced Hb and increased RDW. The addition of high dose monensin significantly improved erythrocytic indices compared to the control. Erythrocyte anisocytosis was observed in blood smears of Cd-treated mice corresponding to the increased RDW. WBV was significantly elevated in the experimental groups in the whole range of shear rates compared to the control group and in groups 2 and 3 was lower than in group 1 but remained higher compared to group 4. Correlations were found between WBV and RBC, Hb, Hct, MCV and RDW. The results suggest that hemorheological parameters such as WBV should be monitored in parallel with the hematological parameters when monensin is applied and heavy metal intoxication is suspected.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, BAS, Sofia, Bulgaria. ygluhcheva@hotmail.com
Y
Y
Gluhcheva
I
I
Ivanov
V
V
Atanasov
N
N
Antonova
Ju
J
Ivanova
M
M
Mitewa
D000085:Acetates / Q000494:pharmacology / Q000633:toxicity*; D000740:Anemia / Q000097:blood / Q000139:chemically induced* / Q000188:drug therapy; D000818:Animals; D000931:Antidotes / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001809:Blood Viscosity / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology / Q000633:toxicity*; D002908:Chronic Disease; D004785:Environmental Pollutants / Q000494:pharmacology / Q000633:toxicity*; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D004913:Erythrocytes, Abnormal; D006454:Hemoglobins / Q000032:analysis; D018056:Hemorheology; D007476:Ionophores / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008985:Monensin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
D000085:Acetates; D000931:Antidotes; D004785:Environmental Pollutants; D006454:Hemoglobins; D007476:Ionophores; D002104:Cadmium; D008985:Monensin; C028031:cadmium acetate