Neuronal damage in hippocampal subregions induced by various durations of transient cerebral ischemia in gerbils using Fluoro-Jade B histofluorescence.
Although there are many studies on ischemic brain damage in the gerbil, which is a good model of transient cerebral ischemia, studies on neuronal damage according to the duration of ischemia-reperfusion (I-R) time are limited. We carried out neuronal damage in the gerbil hippocampus after various durations of I-R (5, 10, 15 and 20 min) using Fluoro-Jade B (F-J B, a maker for neuronal degeneration) histofluorescence as well as cresyl violet (CV) staining. The changes of CV positive ((+)) neurons were well detected in the hippocampal CA1 region, not in the other regions. F-J B histofluorescence staining showed apparent neuronal damage in all the hippocampal subregions. In the CA1, most of the pyramidal neurons of the stratum pyramidale (SP) were stained with F-J B (about 100/mm(2) in a section), and F-J B(+) neurons in the other ischemia-groups were not changed. In the CA2, a few F-J B(+) neurons were detected in the SP of the 5 min ischemia-group, and F-J B(+) neurons were gradually increased with the longer time of ischemia: in the 20 min ischemia-group, the mean number of F-J B(+) neurons was about 85/mm(2) in a section. In the CA3, some F-J B(+) neurons were observed only in the SP of the 20 min ischemia-group. In the dentate gyrus, some F-J B positive neurons were detected only in the polymorphic layer (PL) of the 5 min ischemia-group, and the number of F-J B(+) neurons were gradually increased with the longer ischemic time. Our findings indicate that F-J B histofluorescence showed a very high quality of neuronal damage in all the hippocampal subregions.
Department of Neurosurgery, Seoul Wooridul Hospital, 676 Gwahae-Dong, Gangseo-gu, Seoul 157-822, South Korea.
Dong-Kun
DK
Yu
Ki-Yeon
KY
Yoo
Bich Na
BN
Shin
In Hye
IH
Kim
Joon Ha
JH
Park
Choong Hyun
CH
Lee
Jung Hoon
JH
Choi
Yong-Jun
YJ
Cho
Il-Jun
IJ
Kang
Young-Myeong
YM
Kim
Moo-Ho
MH
Won
D000818:Animals; D016923:Cell Death / Q000502:physiology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005849:Gerbillinae; D006624:Hippocampus / Q000737:chemistry / Q000473:pathology*; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000473:pathology*; D009930:Organic Chemicals
D005452:Fluoresceins; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C435731:fluoro jade