Cyclosporin safety in a simplified rat brain tumor implantation model.
Brain cancer is the second neurological cause of death. A simplified animal brain tumor model using W256 (carcinoma 256, Walker) cell line was developed to permit the testing of novel treatment modalities. Wistar rats had a cell tumor solution inoculated stereotactically in the basal ganglia (right subfrontal caudate). This model yielded tumor growth in 95% of the animals, and showed absence of extracranial metastasis and systemic infection. Survival median was 10 days. Estimated tumor volume was 17.08 ± 6.7 mm(3) on the 7(th) day and 67.25 ± 19.8 mm(3) on 9(th) day post-inoculation. Doubling time was 24.25 h. Tumor growth induced cachexia, but no hematological or biochemical alterations. This model behaved as an undifferentiated tumor and can be promising for studying tumor cell migration in the central nervous system. Dexamethasone 3.0 mg/kg/day diminished significantly survival in this model. Cyclosporine 10 mg/kg/day administration was safely tolerated.
Pediatric hemato-oncologist, Hospital Infantil Albert Sabin, Fortaleza, CE, Brazil. helderfelix@hias.ce.gov.br
Francisco H C
FH
Felix
Juvenia B
JB
Fontenele
Milena G
MG
Teles
João E
JE
Bezerra Neto
Márcia H A M
MH
Santiago
Roberto L
RL
Picanço Filho
Dalgimar B de
DB
Menezes
Glauce S B
GS
Viana
Manoel O de
MO
Moraes
D000704:Analysis of Variance; D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy*; D002279:Carcinoma 256, Walker / Q000188:drug therapy*; D045744:Cell Line, Tumor; D016572:Cyclosporine / Q000008:administration & dosage*; D003907:Dexamethasone / Q000008:administration & dosage; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008297:Male; D009368:Neoplasm Transplantation / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013238:Stereotaxic Techniques
D007166:Immunosuppressive Agents; D003907:Dexamethasone; D016572:Cyclosporine