Improvement of modified charcoal-cefoperazone-deoxycholate agar by supplementation with a high concentration of polymyxin B for detection of Campylobacter jejuni and C. coli in chicken carcass rinses.
Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with a high concentration of polymyxin B. The ability of the supplemented medium to isolate Campylobacter jejuni and C. coli from chicken carcass rinses was compared to that of Campy-Cefex agar and mCCDA. Modification of mCCDA with increased polymyxin B yielded a significantly (P < 0.05) higher isolation rate and greater selectivity than those achieved using Campy-Cefex agar and mCCDA.
Department of Public Health, Konkuk University, Gwangjin-gu, Seoul, Republic of Korea.
Jung-Whan
JW
Chon
Ji-Yeon
JY
Hyeon
Jin-Hyuk
JH
Yim
Jong-Hyun
JH
Kim
Kwang-Young
KY
Song
Kun-Ho
KH
Seo
D000362:Agar; D000818:Animals; D001431:Bacteriological Techniques; D017000:Campylobacter coli / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D002438:Cefoperazone / Q000378:metabolism; D002606:Charcoal / Q000378:metabolism; D002645:Chickens; D003470:Culture Media / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000378:metabolism; D011112:Polymyxin B / Q000378:metabolism; D012641:Selection, Genetic; D012680:Sensitivity and Specificity
D003470:Culture Media; D003840:Deoxycholic Acid; D002606:Charcoal; D002438:Cefoperazone; D000362:Agar; D011112:Polymyxin B