Enteractinococcus coprophilus gen. nov., sp. nov., of the family Micrococcaceae, isolated from Panthera tigris amoyensis faeces, and transfer of Yaniella fodinae Dhanjal et al. 2011 to the genus Enteractinococcus as Enteractinococcus fodinae comb. nov.
A novel actinobacterium, designated strain YIM 100590(T), was isolated from Panthera tigris amoyensis faeces collected from Yunnan Wild Animal Park in Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 100590(T) is a member of the family Micrococcaceae. Cells were coccoid to oval (0.7-1.5 µm in diameter) occurring singly or in clusters. Growth was observed at 10-37 °C (optimum 28 °C) and at pH 7.0-11.0 (optimum pH 8.0). The major fatty acids were iso-C(15:0) (32.22%), anteiso-C(15:0) (31.64%) and iso-C(16:0) (17.38%). The peptidoglycan was of A4α type (L-Lys-Gly-L-Glu). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, dimannosyl diacylglycerol, an unknown glycolipid and two unknown phospholipids. The quinone system comprised menaquinones MK-7 (91.9%) and MK-8 (8.3%). The DNA G+C content of strain YIM 100590(T) was 56.2 mol%. Chemotaxonomic data indicated that the strain belongs to the family Micrococcaceae. On the basis of morphological and chemotaxonomic data and phylogenetic analysis, strain YIM 100590(T) is considered to represent a novel species of a new genus within the family Micrococcaceae, for which the name Enteractinococcus coprophilus gen. nov., sp. nov. is proposed. The type strain of Enteractinococcus coprophilus is YIM 100590(T) (=DSM 24083(T)=JCM 17352(T)). Yaniella fodinae DSM 22966(T) was transferred to the new genus as Enteractinococcus fodinae comb. nov. (type strain G5(T)=DSM 22966(T)=JCM 17931(T)=MTCC 9846(T)).
Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.|College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
Yan-Ru
YR
Cao
Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Yi
Y
Jiang
Yunnan Institute of Microbiology, Yunnan University, 650091 Kunming, PR China.
Rong-Xian
RX
Jin
Laboratory of Metabolic Disease Research and Drug Development, China Medical University, 110001 Shenyang, PR China.
Li
L
Han
College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
Wen-Xiang
WX
He
Yunnan Wild Animal Park, 650218 Kunming, PR China.
You-Long
YL
Li
Laboratory of Metabolic Disease Research and Drug Development, China Medical University, 110001 Shenyang, PR China.
Xue-Shi
XS
Huang
College of Resources and Environment, Northwest A & F University, 712100 Yangling, Shaanxi, PR China.
Quan-Hong
QH
Xue
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D046010:Tigers / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S