[Nuclear texture in mitotic cells].
Changes of nuclear texture in mitotic cells of Drosophila melanogaster imaginal discs were studied. The distribution of voxels DAPI fluorescence intensities was used as the quantitative measure of the nuclear texture. The integral characteristics such as the portion of voxels with a given fluorescent signal level and autocorrelation of pixel intensities were used. We showed the nuclear texture has specific changes at different mitotic stages and this can be used for more precise staging of mitosis. Colchicines treatment pathologies, connected to abnormal mitoses, by nuclear-texture approach.
L I
LI
Lebedeva
T D
TD
Dubatolova
L V
LV
Omel'ianchuk
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D003078:Colchicine / Q000009:adverse effects; D004331:Drosophila melanogaster / Q000166:cytology* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis; D006657:Histones / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D060227:Imaginal Discs / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000032:analysis*; D007814:Larva / Q000166:cytology* / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000187:drug effects
D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; C007293:DAPI; D003078:Colchicine