Double-stranded RNA induces S100 gene expression by a cycloheximide-sensitive factor.
Viral double-stranded RNA (dsRNA) and its synthetic analog polyI:C are recognized via multiple pathways and induce the expression of genes related to inflammation. In the present study, we demonstrated the polyI:C-induced gene expression of the damage associated molecular pattern (DAMP) molecules S100A8 and S100A9, while other S100 genes were not affected. Cycloheximide and Brefeldin A treatment revealed both the expression of S100A8 and S100A9 as secondary response genes and the involvement of polyI:C-induced cytokines herein. Several type I and type III interferons such as IFNβ, IL-20, IL-24, and IFNλ/IL-29 were expressed in response to polyI:C, however, they failed to induce S100A8 and S100A9 gene expression. These data indicate the involvement of the danger molecule S100A8/A9 in the resistance against viruses.
Institute of Immunology, University of Witten-Herdecke, Germany.
Andreas
A
Voss
Kirsten
K
Gescher
Andreas
A
Hensel
Wolfgang
W
Nacken
Kurt S
KS
Zänker
Claus
C
Kerkhoff
D056704:Adaptive Immunity / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D020126:Brefeldin A / Q000494:pharmacology; D040501:Calgranulin A / Q000235:genetics / Q000378:metabolism; D040502:Calgranulin B / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011070:Poly I-C / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000494:pharmacology / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics / Q000502:physiology; D009418:S100 Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation / Q000187:drug effects
D040501:Calgranulin A; D040502:Calgranulin B; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D009418:S100 Proteins; D014157:Transcription Factors; D020126:Brefeldin A; D003513:Cycloheximide; D011070:Poly I-C