Microarray technology for yeast identification directly from positive blood cultures. A multicenter Italian experience.
The authors evaluated the performance of the MycArray™ Yeast ID (Myconostica Ltd, UK) assay in the identification of a total of 88 yeast isolates recovered in culture as compared to that obtained through routine methods. The turn-around time for species identification directly from cultures by the MycArray was 6 hours, much quicker than classical methods and all yeasts were correctly identified. In two cases a double identification including Saccharomyces cerevisiae was noted, but it was not confirmed by culture. The results show that MycArray Yeast ID can be a potential tool for rapid detection and identification of Candida species.
Medical Mycology Committee, Italian Society of Clinical Microbiology, Milano, Italy. farina.claudio@sancarlo.mi.it
Claudio
C
Farina
Giuseppe
G
Russello
Stefano
S
Andreoni
Cristina
C
Bonetti
Marco
M
Conte
Paolo
P
Fazi
Gianluigi
G
Lombardi
Francesco
F
Luzzaro
Esther
E
Manso
Piero
P
Marone
Marco
M
Passera
Andrea
A
Rocchetti
Silvana
S
Sanna
Egidio Franco
EF
Viganò
D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D016469:Fungemia / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D007558:Italy; D016533:Mycological Typing Techniques / Q000295:instrumentation / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media