Simultaneous quantitation of urinary cotinine and acrylonitrile-derived mercapturic acids with ultraperformance liquid chromatography-tandem mass spectrometry.
Acrylonitrile (AN), a widely used industrial chemical also found in tobacco smoke, has been classified as a possible human carcinogen (group 2B) by the International Agency for Research on Cancer. AN can be detoxified by glutathione S-transferase (GST) to form glutathione (GSH) conjugates in vivo. It can be metabolically activated by cytochrome P450 2E1 to form 2-cyanoethylene oxide, which can also be detoxified by GST to generate GSH conjugates. The GSH conjugates can be further metabolized to mercapturic acids (MAs), namely, N-acetyl-S-(2-cyanoethyl)cysteine (CEMA), N-acetyl-S-(2-hydroxyethyl)cysteine (HEMA), and N-acetyl-S-(1-cyano-2-hydroxyethyl)cysteine (CHEMA). This study developed an ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method to quantitatively profile the major AN urinary metabolites (CEMA, HEMA, and CHEMA) to assess AN exposure, as well as analyze urinary cotinine (COT) as an indicator for tobacco smoke exposure. The limits of quantitation were 0.1, 0.1, 1.0, and 0.05 μg/L for HEMA, CEMA, CHEMA, and COT, respectively. This method was applied to analyze the three AN-derived MAs in 36 volunteers with no prior occupational AN exposure. Data analysis showed significant correlations between the level of COT and the levels of these MAs, suggesting them as biomarkers for exposure to low levels of AN. The results demonstrate that a highly specific and sensitive UPLC-MS/MS method has been successfully developed to quantitatively profile the major urinary metabolites of AN in humans to assess low AN exposure.
Department of Public Health & Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan.
Chia-Fang
CF
Wu
Shi-Nian
SN
Uang
Su-Yin
SY
Chiang
Wei-Chung
WC
Shih
Yu-Fang
YF
Huang
Kuen-Yuh
KY
Wu
D000111:Acetylcysteine / Q000378:metabolism / Q000652:urine*; D000181:Acrylonitrile / Q000378:metabolism / Q000652:urine*; D002273:Carcinogens / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003367:Cotinine / Q000378:metabolism / Q000652:urine*; D006801:Humans; D012680:Sensitivity and Specificity; D012907:Smoking / Q000378:metabolism / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002273:Carcinogens; D003367:Cotinine; D000181:Acrylonitrile; D000111:Acetylcysteine