Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing.
Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Oil Crop Biology and Genetic Breeding of the Ministry of Agriculture, Wuhan, 430062, China.
Cai-Hua
CH
Dong
Chen
C
Li
Xiao-Hong
XH
Yan
Shun-Mou
SM
Huang
Jin-Yong
JY
Huang
Li-Jun
LJ
Wang
Rui-Xing
RX
Guo
Guang-Yuan
GY
Lu
Xue-Kun
XK
Zhang
Xiao-Ping
XP
Fang
Wen-Hui
WH
Wei
D016000:Cluster Analysis; D015536:Down-Regulation / Q000235:genetics; D055864:Droughts; D018628:Gene Dosage / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D031226:Sinapis / Q000235:genetics* / Q000254:growth & development*; D013312:Stress, Physiological / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics; D014867:Water
D014867:Water