Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus.
Clarias batrachus, an Indian catfish species, is endemic to the Indian subcontinent and potential cultivable species. The genomic resources in C. batrachus in the form of ESTs containing microsatellite repeats (EST-SSR) and single nucleotide polymorphisms (SNPs) that are associated with the expressed genes from spleen were mined. From a total of 1,937 ESTs generated, 1,698 unique sequences were obtained, out of which 221 EST-SSRs were identified and 54% could be functionally annotated by similarity searches. A total of 23 contigs containing 3 or more ESTs were found to contain 31 SNP loci, out of which 8 ESTs showed similarity to genes of known function and 1 for hypothetical protein. Nine ESTs with SSRs and/or SNPs identified in this study were reported to be associated with diseases in human and animals. These identified loci can be developed into markers in C. batrachus, which can be useful in linkage mapping, comparative genomics studies and for its genetic improvement programmes.
National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow, 226 002, UP, India. vindhyamohindra@gmail.com
Vindhya
V
Mohindra
Akanksha
A
Singh
A S
AS
Barman
Ratnesh
R
Tripathi
Neeraj
N
Sood
Kuldeep K
KK
Lal
D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics* / Q000276:immunology; D018900:Dinucleotide Repeats / Q000235:genetics; D020224:Expressed Sequence Tags / Q000378:metabolism*; D056426:Genetic Loci / Q000235:genetics; D016678:Genome / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006801:Humans; D007194:India; D018895:Microsatellite Repeats / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013154:Spleen / Q000378:metabolism; D018911:Trinucleotide Repeats / Q000235:genetics