Novel microbial transformation of resibufogenin by Absidia coerules.
Resibufogenin is one of the major active components of the Chinese medicine ChanSu. In this paper, microbial transformation of resibufogenin by Absidia coerules AS 3.3382 was investigated and five metabolites were isolated and identified as 5beta-hydroxy-resibufogenin (2), 3-epi-resibufogenin (3), 3alpha-hydroxy-15-oxo-14alphaH-bufa-20, 22-dienolide (4), 3alpha,14alpha,15beta-trihydroxy-bufa-20, 22-dienolide (5) and 3-epi-15beta-hydroxy-bufalin (6). Among them, 4-6 are novel compounds, and compound 3 is a major transformed product. The cytotoxicities of the compounds against Bel-7402 and Hela cells were investigated, and our result suggested that 14,15-OH configuration was directly related to the cytotoxicities of bufadienolides.
Division of Chinese Materia Medica & Natural products, National Institute for Control of Pharmaceutical & Biological Products, Beijing 100050, China.
Jian
J
Zheng
Dong-Hai
DH
Su
Dong-Sheng
DS
Zhang
Xiu-Lan
XL
Xin
Jun-Ying
JY
Liu
Yan
Y
Tian
Qing
Q
Wei
Xun
X
Cui
D020095:Absidia / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000032:analysis; D001711:Biotransformation; D002018:Bufanolides / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy
D000970:Antineoplastic Agents; D002018:Bufanolides; D004365:Drugs, Chinese Herbal; C005734:bufogenin