Trichinella spiralis-secreted products modulate DC functionality and expand regulatory T cells in vitro.
Helminths and their products can suppress the host immune response which may benefit parasite survival. Trichinella spiralis can establish chronic infections in a wide range of mammalian hosts including humans and mice. Here, we aim at studying the effect of T. spiralis muscle larvae excretory/secretory products (TspES) on the functionality of DC and T cell activation. We found that TspES suppress in vitro DC maturation induced by both S- and R-form lipopolysaccharide(LPS) from enterobacteria. Using different toll-like receptor (TLR) agonists, we show that the suppressive effect of TspES on DC maturation is restricted to TLR4. These helminth products also interfere with the expression of several genes related to the TLR-mediated signal transduction pathways. To investigate the effect of TspES on T cell activation, we used splenocytes derived from OVA-TCR transgenic D011.10 that were incubated with OVA and TspES-pulsed DC. Results indicate that the presence of TspES resulted in the expansion of CD4(+) CD25(+) Foxp3+ T cells. These regulatory T (Treg) cells were shown to have suppressive activity and to produce TGF-β. Together these results suggest that T. spiralis secretion products can suppress DC maturation and induce the expansion of functional Treg cells in vitro.
Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
C
C
Aranzamendi
F
F
Fransen
M
M
Langelaar
F
F
Franssen
P
P
van der Ley
J P M
JP
van Putten
V
V
Rutten
E
E
Pinelli
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D057809:HEK293 Cells; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D056747:Immunomodulation; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology*; D051193:Toll-Like Receptors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D017160:Trichinella spiralis / Q000276:immunology* / Q000378:metabolism; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology
D000947:Antigens, Helminth; D015801:Helminth Proteins; D051193:Toll-Like Receptors; D016212:Transforming Growth Factor beta