Monitoring of cellular senescence by DNA-methylation at specific CpG sites.
Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long-term culture based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture.
Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Aachen, Germany.
Carmen M
CM
Koch
Sylvia
S
Joussen
Anne
A
Schellenberg
Qiong
Q
Lin
Martin
M
Zenke
Wolfgang
W
Wagner
D002478:Cells, Cultured; D016922:Cellular Senescence; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006801:Humans