Interaction of protein inhibitor of activated STAT 2 (PIAS2) with receptor of activated C kinase 1, RACK1.
In this study, the evolutionarily conserved intracellular adaptor protein, receptor of activated C kinase 1 (RACK1) was identified as a novel interaction partner of protein inhibitor of activated STAT 2 (PIAS2) using a yeast two-hybrid screening system. The direct interaction and co-localization of RACK1 with PIAS2 was confirmed by immunoprecipitation and immunofluorescence staining analysis, respectively. The 5th to 7th Trp-Asp 40 (5-7 WD40) repeats of RACK1 were identified as the minimal domain required for interaction with PIAS2 by deletion analysis. Furthermore, multiple PIAS2-domains, particularly the 'PINIT' and RLD domains, bind the RACK1 5-7 WD40 domain.
Department of Histology and Embryology, Medical College, Yangzhou University, Yangzhou 225001, China. yzzkl@163.com
Ying
Y
Zheng
Luping
L
Zhang
Xiaoqing
X
Jia
Haiyan
H
Wang
Yanqiu
Y
Hu
D000818:Animals; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs / Q000235:genetics / Q000502:physiology; D060066:Protein Interaction Maps; D000075370:Receptors for Activated C Kinase; D014018:Tissue Distribution; D014162:Transfection; D020798:Two-Hybrid System Techniques
D009479:Neuropeptides; D050828:Protein Inhibitors of Activated STAT; C090294:RACK1 protein, mouse; D000075370:Receptors for Activated C Kinase