Candida adriatica sp. nov. and Candida molendinolei sp. nov., two yeast species isolated from olive oil and its by-products.
Thirteen strains isolated from virgin olive oil or its by-products in several Mediterranean countries were found to be phenotypically and genetically divergent from currently recognized yeast species. Sequence analysis of the large subunit (LSU) rDNA D1/D2 domain and internal transcribed spacer regions/5.8S rDNA revealed that the strains represented two novel species described as Candida adriatica sp. nov. (type strain ZIM 2334(T) = CBS 12504(T) = NCAIM Y.02001(T)) and Candida molendinolei sp. nov. (type strain DBVPG 5508(T) = CBS 12508(T) = NCAIM Y.02000(T)). Phylogenetic analysis based on concatenated sequences of the small subunit rRNA gene, the D1/D2 region of the LSU rDNA and the translation elongation factor-1α gene suggested that C. adriatica sp. nov. and C. molendinolei sp. nov. should be placed within the Lindnera and Nakazawaea clades, respectively.
Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Neža
N
Čadež
Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Peter
P
Raspor
Department of Applied Biology, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.|DBVPG Industrial Yeasts Collection, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
Benedetta
B
Turchetti
Department of Applied Biology, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
Gianluigi
G
Cardinali
Department of Animal, Plant and Environmental Sciences, Agriculture Faculty, University of Molise, Via De Sanctis, I-86100 Campobasso, Italy.
Gino
G
Ciafardini
Department of Economics and Food Science, University of Perugia, Borgo XX Giugno 74, I-06121 Perugia, Italy.
Gianluca
G
Veneziani
National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Sciences, Corvinus University of Budapest, Somlói út 14-16, H-1118 Budapest, Hungary.
Gábor
G
Péter
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005516:Food Microbiology; D020459:Genes, rRNA; D016533:Mycological Typing Techniques; D031658:Olea / Q000382:microbiology*; D000069463:Olive Oil; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010938:Plant Oils; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D000069463:Olive Oil; D020648:Peptide Elongation Factor 1; D010938:Plant Oils; D012340:RNA, Ribosomal, 5.8S