[The efficacy of enhancing murine zona pellucid 3 DNA vaccine immunized through intranasal vaccination by IL-15].
OBJECTIVE
To observe the efficacy of enhancing murine zona pellucid 3(mZP3) DNA vaccine immunized through intranasal vaccination by IL-15 as adjuvant and chitosan as delivery vactor.


METHODS
C57BL/6 mice were delivered with chitosan-loaded mZP3 DNA vaccine or IL-15+pcD-mZP3 vaccine by intranasal route,  and to detect specific IgG in serum and sIgA in vaginal fluid by indirect ELISA, and to perform fertility experiment that all female C57BL/6 mice  were mated(one male for each female) with normal males of similar age. To do histology analysis of immunized mice ovary and lungs for morphology of ovary.


RESULTS
The results of ELISA showed IL-15 can promote IgG antibody level of immunized mice,  and the fertility rate was decreased to some degrees.


CONCLUSIONS
IL-15 and chitosan can enhance the antibody levels induced by mZP3 DNA vaccine,  and reduce murine fertility rate to a certain extent.
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. liufupei2008@sina.com
Fu-pei
FP
Liu
Dan
D
Chang
Yan-kun
YK
Yu
Fu-chun
FC
Zhang
Ai-lian
AL
Zhang
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D004527:Egg Proteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D005298:Fertility / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D019409:Interleukin-15 / Q000008:administration & dosage / Q000494:pharmacology*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D000276:Adjuvants, Immunologic; D004527:Egg Proteins; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D019409:Interleukin-15; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D000072216:Zona Pellucida Glycoproteins