Investigation on ultrasound-assisted extraction and separation of enduracidin from Streptomyces sp. NJWGY3665.
Ultrasound-assisted extraction of enduracidin from Streptomyces sp. NJWGY3665 was studied. The effects of various factors on the yield of target components were investigated. The results showed that the extraction by ultrasound-assisted extraction is four times faster than those by conventional solvent extraction. The results also indicated that fast extraction rate was obtained in the first 30 min, and the maximum yield was obtained at the power of 150 W. The effects of other factors such as different solvents, solvent concentration, solvent to solid ratio, and extraction batches on the field were also discussed. The optimum conditions were found at solvent of methanol, time of 30 min, power of 150 W, pH of 7.0, solvent to solid ratio of 50 ml/g, solvent concentration of 70% and extraction batches of four times.
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No.200, North Zhongshan Road, Nanjing 210009, People's Republic of China. yonghonghu09@gmail.com
Yonghong
Y
Hu
Wenge
W
Yang
Wenqian
W
Wan
Fei
F
Shen
Ziyu
Z
Lei
Dandan
D
Wang
D000900:Anti-Bacterial Agents / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D059625:Liquid-Liquid Extraction; D010456:Peptides, Cyclic / Q000302:isolation & purification*; D012997:Solvents; D013010:Sonication; D013302:Streptomyces / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010456:Peptides, Cyclic; D012997:Solvents; C505063:enduracidin A; C505064:enduracidin B