Bone regeneration in a rabbit critical-sized calvarial model using tyrosine-derived polycarbonate scaffolds.
Porous three-dimensional tyrosine-derived polycarbonate (TyrPC) scaffolds with a bimodal pore distribution were fabricated to mimic bone architecture using a combination of salt-leaching and phase separation techniques. TyrPC scaffolds degraded in register with bone regeneration during the 6-week study period and compressive moduli of the scaffolds were maintained >0.5 MPa at 6 weeks of incubation in PBS at 37 °C. The TyrPC scaffolds either unsupplemented or supplemented with recombinant human bone morphogenetic protein-2 (rhBMP-2) were implanted in a rabbit calvarial critical-sized defect (CSD) model and the TyrPC scaffolds treated with rhBMP-2 or TyrPC coated with calcium phosphate scaffold alone promoted bone regeneration in a rabbit calvarial CSD at 6 weeks postimplantation. A synthetic TyrPC polymeric scaffold either without a biological supplement or with a minimal dose of rhBMP-2 induced bone regeneration comparable to a commercially available bone graft substitute in a nonrodent CSD animal model.
Department of Biomedical Engineering, Bone Tissue Engineering Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15219, USA.
Jinku
J
Kim
Maria Hanshella R
MH
Magno
Heather
H
Waters
Bruce A
BA
Doll
Sean
S
McBride
Pedro
P
Alvarez
Aniq
A
Darr
Amit
A
Vasanji
Joachim
J
Kohn
Jeffrey O
JO
Hollinger
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D004195:Disease Models, Animal; D055119:Elastic Modulus / Q000187:drug effects; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D011076:Polycarboxylate Cement / Q000494:pharmacology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014443:Tyrosine / Q000494:pharmacology*; D055114:X-Ray Microtomography
D055396:Bone Morphogenetic Protein 2; D011076:Polycarboxylate Cement; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C005506:polycarbonate; D014443:Tyrosine