Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.
Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.
Johns Hopkins University, Baltimore, Maryland USA. kyj2@duke.edu
Kevin
K
Jeng
Samuel
S
Yang
Helen
H
Won
Charlotte A
CA
Gaydos
Yu-Hsiang
YH
Hsieh
Alex
A
Kecojevic
Karen C
KC
Carroll
Justin
J
Hardick
Richard E
RE
Rothman
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012480:Salmonella Infections / Q000175:diagnosis* / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors; D044366:Transition Temperature
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S