Biocompatibility and antimicrobial activity of poly(3-hydroxyoctanoate) grafted with vinylimidazole.
Vinylimidazole-grafted poly(3-hydroxyoctanoate) (VI-g-PHO) copolymers were prepared by heating homogeneous solutions of PHO, VI monomer, and benzoylperoxide initiator. The Fourier transform infrared spectroscopy attenuated total reflection and electron spectroscopy for chemical analyses showed that VI was successfully grafted onto the PHO chains. The surfaces and the bulk of VI-g-PHO copolymers became more hydrophilic as the VI grafting density in the copolymer increased. Measurements of the growth of Chinese hamster ovary cells and the adsorption of blood proteins and platelets in vitro showed that biocompatibility was also enhanced by grafting of VI groups. Antimicrobial activity of the VI-g-PHO copolymers was studied against Escherichia coli, Staphylococcus aureus, and Candida albicans. Treatment of each culture suspension with 2.0% (w/v) copolymers for 12h resulted in >90% reduction in viable cell counts against all test microorganisms. These results indicate that the VI-g-PHO copolymers are promising materials for biomedical applications, as they exhibited both biocompatibility and broad spectrum antimicrobial activity.
Department of Microbiology, Chungnam National University, Daejeon, South Korea.
Moon Gyu
MG
Chung
Hyung Woo
HW
Kim
Bo Ra
BR
Kim
Young Baek
YB
Kim
Young Ha
YH
Rhee
D000327:Adsorption; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D007093:Imidazoles / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D001798:Blood Proteins; D007093:Imidazoles; D011091:Polyesters; C572407:vinylimidazole-grafted poly(3-hydroxyoctanoate)