[NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes.
We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [NSI(+)] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [NSI(+)], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [NSI(+)] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [NSI(+)] and [nsi(-)] strains. We demonstrate that VTS1 overexpression, like [NSI(+)] induction, causes translational read-through and growth defects in S. cerevisiae.
St. Petersburg Branch Vavilov Institute of General Genetics, Russian Academy of Science, Russia.
Anton A
AA
Nizhnikov
Zalina M
ZM
Magomedova
Alexandr A
AA
Rubel
Alexandra M
AM
Kondrashkina
Sergey G
SG
Inge-Vechtomov
Alexey P
AP
Galkin
D001483:Base Sequence; D018389:Codon, Nonsense; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000235:genetics*; D010641:Phenotype; D011328:Prions / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013489:Suppression, Genetic
D018389:Codon, Nonsense; D010454:Peptide Termination Factors; D011328:Prions; D016601:RNA-Binding Proteins; C068068:SUP35 protein, S cerevisiae; C080775:SUP45 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C508663:Vts1 protein, S cerevisiae