Development of FRET-based ratiometric fluorescent Cu2+ chemodosimeters and the applications for living cell imaging.
Coumarin-rhodamine-based compounds 1a,b were rationally designed and synthesized as novel FRET ratiometric fluorescent chemodosimeters. Ratiometric chemodosimeters 1a,b exhibit several favorable features, including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, high selectivity, low cytotoxicity, and good cell membrane permeability. Importantly, these excellent attributes enable us to demonstrate ratiometric imaging of Cu(2+) in living cells by using these novel ratiometric fluorescent chemodosimeters.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, People's Republic of China.
Lin
L
Yuan
Weiying
W
Lin
Bin
B
Chen
Yinan
Y
Xie
D002413:Cations, Divalent / Q000737:chemistry; D002470:Cell Survival; D003300:Copper / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D003300:Copper