P450scc regulation in pig granulosa cells: investigation into the mechanism of induction.
P450scc catalyses the first and rate-limiting reaction in steroidogenesis and is hormonally regulated. By Northern analysis, using a bovine cDNA probe, we have studied the regulation of P450scc mRNA in pig granulosa cells cultivated in vitro. Using transcription and translation inhibitors, we show that the gonadotropin-induced accumulation of P450scc mRNA mainly results from increased transcription, and that this stimulation, at least in part, is protein synthesis-dependent. Although transcriptional regulation of P450scc gene expression is found in other steroidogenic cells, cycloheximide-sensitivity of this regulation is not widespread. Pig granulosa cells thus would constitute a useful model to study this mechanism of regulation.
Laboratoire de Génétique Cellulaire, Centre de Recherches INRA de Toulouse, Castanet-Tolosan, France.
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Hatey
J P
JP
Gasparoux
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Mulsant
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Bonnet
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Gasser
D000818:Animals; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006062:Gonadotropins / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006080:Ovarian Follicle / Q000166:cytology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D015290:Second Messenger Systems / Q000187:drug effects; D034421:Sus scrofa; D014158:Transcription, Genetic / Q000187:drug effects
D006062:Gonadotropins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D005576:Colforsin; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D002786:Cholesterol Side-Chain Cleavage Enzyme