Functional characterization of a synthetic hydrophilic antifungal peptide derived from the marine snail Cenchritis muricatus.
Antimicrobial peptides have been found in mollusks and other sea animals. In this report, a crude extract of the marine snail Cenchritis muricatus was evaluated against human pathogens responsible for multiple deleterious effects and diseases. A peptide of 1485.26 Da was purified by reversed-phase HPLC and functionally characterized. This trypsinized peptide was sequenced by MS/MS technology, and a sequence (SRSELIVHQR), named Cm-p1 was recovered, chemically synthesized and functionally characterized. This peptide demonstrated the capacity to prevent the development of yeasts and filamentous fungi. Otherwise, Cm-p1 displayed no toxic effects against mammalian cells. Molecular modeling analyses showed that this peptide possible forms a single hydrophilic α-helix and the probable cationic residue involved in antifungal activity action is proposed. The data reported here demonstrate the importance of sea animals peptide discovery for biotechnological tools development that could be useful in solving human health and agribusiness problems.
Centro de Estudios de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25 entre J e I, Vedado, Municipio Plaza, La Habana 10400, Cuba.
Carlos
C
López-Abarrategui
Annia
A
Alba
Osmar N
ON
Silva
Osvaldo
O
Reyes-Acosta
Ilka M
IM
Vasconcelos
Jose T A
JT
Oliveira
Ludovico
L
Migliolo
Maysa P
MP
Costa
Carolina R
CR
Costa
Maria R R
MR
Silva
Hilda E
HE
Garay
Simoni C
SC
Dias
Octávio L
OL
Franco
Anselmo J
AJ
Otero-González
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D056148:Chromatography, Reverse-Phase; D004912:Erythrocytes; D005658:Fungi / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017433:Protein Structure, Secondary; D020539:Sequence Analysis, Protein; D012908:Snails; D013499:Surface Properties
D000935:Antifungal Agents; D010446:Peptide Fragments