Identification and characterization of a noncoding RNA at the mouse Pcna locus.
AK007836 encodes a noncoding RNA (ncRNA) consisting of 2 exons. Since AK007836 is located just upstream of Pcna and transcribed in the opposite direction to that of Pcna, we analyzed its expression pattern. Both ncRNA and Pcna expressions were detected in in vitro and in vivo cells, showing a positive correlation. A 177 bp region separating the first exons of Pcna and AK007836 has a bidirectional promoter activity. When the expression of ncRNA was reduced by siRNA, Pcna expression was also reduced in normal cells, but not in cancer cells. These results suggest that the ncRNA is divergently transcribed from the bidirectional promoter, positively regulating the neighboring protein-coding Pcna gene transcription, and this regulatory function is somehow disrupted in cancer cells.
Department of Anatomy, Embryology Laboratory, Yonsei University College of Medicine, Seoul, 120-752, Korea.
Ji-Yeon
JY
Lee
Abdul Aziz
AA
Khan
Hyehyun
H
Min
Xinnan
X
Wang
Myoung Hee
MH
Kim
D000818:Animals; D001483:Base Sequence; D018827:Carcinoma, Lewis Lung; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D056426:Genetic Loci / Q000235:genetics; D008546:Melanoma, Experimental; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D022661:RNA, Untranslated