The effect of taxol microinjection on the microtubular structure in polar body formation of starfish oocytes.
In starfish oocytes, microtubules (MTs) form a spindle, which plays an important role in contributing to the selective loss of chromosomes and centrosomes to the polar bodies (PBs) during meiosis. When Taxol was locally injected near the germinal vesicle (GV) or the mitotic apparatus during meiosis I, PB formation was inhibited as mentioned below. In the oocytes, which were injected with Taxol after spindle formation, the spindle became large, and then the volume of the first PB also increased more than that of the control. In contrast, in the oocytes injected with Taxol before the spindle formation, chromosome capture and alignment were inhibited. These oocytes did not form PB, but only a bulge at the cell cortex was occasionally observed. Moreover, in the oocytes injected with Taxol before GV breakdown, the chromosomes did not gather in one place, and then two asters were observed at distant positions from the cell cortex. These results suggested that MTs lost not only the ability to obtain the bipolar attachment of chromosomes by Taxol injection but also the aster closer to the cell cortex lost its interaction with the cell cortex of the animal pole.
Department of Bioengineering, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551, Japan.
Yohei
Y
Kikuchi
Yukihisa
Y
Hamaguchi
D000818:Animals; D047288:Asterina; D005260:Female; D049469:Meiotic Prophase I / Q000187:drug effects*; D008845:Microinjections / Q000379:methods; D008870:Microtubules / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology*; D059705:Polar Bodies / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D050257:Tubulin Modulators / Q000494:pharmacology*
D050257:Tubulin Modulators; D017239:Paclitaxel