CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) producing ROS affects growth and viability of filamentous fungi.
CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) causes intracellular superoxide production and oxidative stress and enhances the susceptibility of Saccharomyces cerevisiae, Candida albicans, and C. glabrata cells to cycloheximide, 5-fluorocytosine, and azole antimycotic drugs. Here, we demonstrate the antifungal activity of CTBT against 14 tested filamentous fungi. CTBT prevented spore germination and mycelial proliferation of Aspergillus niger and the pathogenic Aspergillus fumigatus. The action of CTBT is fungicidal. CTBT increased the formation of reactive oxygen species in fungal mycelium as detected by 2',7'-dichlorodihydrofluorescein diacetate and reduced the radial growth of colonies in a dose-dependent manner. Co-application of CTBT and itraconazole led to complete inhibition of fungal growth at dosages lower than the chemicals alone. Antifungal and chemosensitizing activities of CTBT in filamentous fungi may be useful in combination treatments of infections caused by drug-resistant fungal pathogens.
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic.
Hana
H
Culakova
Vladimira
V
Dzugasova
Yvetta
Y
Gbelska
Julius
J
Subik
D000935:Antifungal Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000378:metabolism; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development*; D025301:Hyphae / Q000187:drug effects; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development; D014227:Triazines / Q000494:pharmacology*
C518245:7-chlorotetrazolo(5,1-c)benzo(1,2,4)triazine; D000935:Antifungal Agents; D003470:Culture Media; D017382:Reactive Oxygen Species; D014227:Triazines; D017964:Itraconazole