Molecular diagnosis of subcutaneous Pythium insidiosum infection by use of PCR screening and DNA sequencing.
Pythium insidiosum is an emerging human pathogen classified among brown algae and diatoms that can cause significant morbidity and mortality in otherwise healthy individuals. Here we describe a pediatric patient with pythiosis acquired in the southern United States, diagnosed by molecular screening and DNA sequencing of internal transcribed spacer region 1.
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA. stevesal@uw.edu
Stephen J
SJ
Salipante
Daniel R
DR
Hoogestraat
Dhruba J
DJ
SenGupta
Donald
D
Murphey
Kyriacos
K
Panayides
Emma
E
Hamilton
Irene
I
Castañeda-Sánchez
Jason
J
Kennedy
Peter W
PW
Monsaas
Leonel
L
Mendoza
Karen
K
Stephens
James J
JJ
Dunn
Brad T
BT
Cookson
D000293:Adolescent; D000671:Amputation, Surgical; D001483:Base Sequence; D005260:Female; D006801:Humans; D007866:Leg / Q000469:parasitology* / Q000601:surgery; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D058968:Pythiosis / Q000175:diagnosis* / Q000469:parasitology; D011775:Pythium; D017422:Sequence Analysis, DNA