Simultaneous purification and site-specific modification of pyrroline-carboxy-lysine proteins.
Sticky residue: Pyrroline-carboxy-lysine (Pcl) can be readily incorporated into proteins expressed in E. coli and mammalian cells by using the pyrrolysyl tRNA/tRNA synthetase pair. Pcl can be used as a single amino acid purification tag and can be site-specifically modified with functional probes during the elution process.
Genomics Institute of the Novartis Research Foundation, 10675 John-Jay-Hopkins Drive, San Diego, CA 92121, USA.
Hsien-Po
HP
Chiu
Jan
J
Grünewald
Xueshi
X
Hao
Ansgar
A
Brock
Linda
L
Okach
Tetsuo
T
Uno
Bernhard H
BH
Geierstanger
D001547:Benzaldehydes / Q000737:chemistry; D001665:Binding Sites; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
C561311:2-amino-6-(3,4-dihydro-2H-pyrrole-2-carboxamido)hexanoic acid; D001547:Benzaldehydes; D011506:Proteins; C000062:2-aminobenzaldehyde; D008239:Lysine