Molecular identification of larval bucephalids, Prosorhynchoides ozakii and Parabucephalopsis parasiluri , infecting the golden mussel, Limnoperna fortunei , by PCR-RFLP.
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was developed for the molecular identification of 2 introduced bucephalid trematodes, Prosorhynchoides ozakii and Parabucephalopsis parasiluri . The method was applied for sporocysts and cercariae obtained from the golden mussel Limnoperna fortunei collected in the Uji River, Japan. The PCR-RFLP method showed that L. fortunei is the intermediate host of both trematode species. The present study thus recognizes the risk of L. fortunei , an invasive molluscan species, as a potential host for pathogenic trematodes.
Department of Ecosystem Studies, School of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka, Hikone, Shiga, Japan. f11tbaba@ec.usp.ac.jp
Takashi
T
Baba
Daigo
D
Nakamura
Masatomi
M
Hosoi
Misako
M
Urabe
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000378:metabolism; D004275:DNA, Ribosomal / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014200:Trematoda / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D018509:DNA, Helminth; D004275:DNA, Ribosomal; C047020:endodeoxyribonuclease ScaI; D015252:Deoxyribonucleases, Type II Site-Specific