[Comparative study of the cytotoxicity induced by chrysotile asbestos, rock wool and substitute fibers in vitro].
OBJECTIVE
To study the cytotoxicity induced by chrysotile asbestos (CA), rock wool (RW) and wollastonite (WS).


METHODS
V79 cells were divided into 4 groups. i.e. CA group, WS group, RW group and control group (200 microl PBS). The exposure concentration of dusts was 100 mg/L, The cell viability was detected by MTT and lactate dehydrogenase (LDH) activity assays. The technique of scanning electron microscopy was used to examine the change of V79 cells.


RESULTS
SiO2 was main constituent for 3 kinds of dusts. In MTT assay, the cell viability of RW and WS groups was 64.8% and 65.7%, respectively, which were significantly higher than that (54.5%) of CA group (P < 0.01). In LDH assay, the LDH activity of RW and WS groups [(15.7 +/- 50.9), (12.3 +/- 3.7) U/L, respectively] was significantly lower than that [(20.2 +/- 0.9) U/L] of CA group (P < 0.05). In scanning electron microscopy examination, it was found that the two ends of V79 cells in CA group contained a great deal of fibers remaining bodies, but the V79 cell appearance in RW and WS groups was normal.


CONCLUSIONS
The cytotoxicity induced by RW and WS is significantly lower than that induced by CA for V79 cell.
The Mianyang #404 Hospital, Sichuan Province 621000, China.
Jian-Jun
JJ
Deng
Fa-Qin
FQ
Dong
Li-Ming
LM
Wang
Si-Yang
SY
Gan
Jian
J
Liu
Ya-Li
YL
Zeng
D000818:Animals; D017632:Asbestos, Serpentine / Q000633:toxicity*; D017610:Calcium Compounds / Q000633:toxicity*; D002460:Cell Line / Q000187:drug effects; D006224:Cricetinae; D003603:Cytotoxins / Q000633:toxicity*; D045022:Lactate Dehydrogenases / Q000378:metabolism; D019016:Mineral Fibers / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*
D017632:Asbestos, Serpentine; D017610:Calcium Compounds; D003603:Cytotoxins; D019016:Mineral Fibers; D017640:Silicates; D045022:Lactate Dehydrogenases; C031293:calcium silicate