Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.
Asthma is a chronic allergic disorder characterised by chronic inflammation. The balance of type I and type II (CD4+) T helper cells is of critical importance. In asthma there is an overexpression of T(H)2 cytokines, such as IL-4, IL-5 and IL-13. The genes encoding these cytokines are located together the same chromosomal region, 5q31.1 in humans. Here we confirm a central role for the transcription factors NFAT and GATA3 in the regulation of human IL-4 and IL-13. Chromatin Conformation Capture (3C) demonstrated the formation of specific ligation products containing spliced IL-4 and IL-13 DNA sequences in human T(H)2 polarised HuT-78 cells. This suggests that co-ordinate expression of T(H)2 cytokines, under the control of GATA3 and NFAT1 is due to the formation of a chromatin hub by DNA looping.
Department of Respiratory Disease, The First Affiliated Hospital of Nanjing Medical University, China.
Xin
X
Yao
Wangjian
W
Zha
Wei
W
Song
Haiyan
H
He
Mao
M
Huang
Elen
E
Jazrawi
Paul
P
Lavender
Peter J
PJ
Barnes
Ian M
IM
Adcock
Andrew L
AL
Durham
D001249:Asthma / Q000276:immunology*; D001483:Base Sequence; D018106:CD28 Antigens / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000235:genetics; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D047468:Immunoprecipitation; D018793:Interleukin-13 / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008213:Lymphocyte Activation / Q000235:genetics; D008969:Molecular Sequence Data; D050778:NFATC Transcription Factors / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D017455:Receptors, IgE / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
D018106:CD28 Antigens; D002843:Chromatin; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D018793:Interleukin-13; D050778:NFATC Transcription Factors; C494006:NFATC2 protein, human; D017455:Receptors, IgE; D015847:Interleukin-4; D004247:DNA