Isolation and identification of C-19 fatty acids with anti-tumor activity from the spores of Ganoderma lucidum (reishi mushroom).
We previously showed that ethanolic extracts of spores of Ganoderma lucidum inhibit tumor cell proliferation and induce apoptosis of HL-60 cells. The active constituents appeared to be long-chain fatty acids, particularly carbon-19 (C-19) fatty acids which have not been reported in spores of Ganoderma lucidum. In the present study, two of these C-19 fatty acids which are key compounds in the activities, were identified as their 2-naphthyl ester derivatives after esterification of a mixture of fatty acids obtained from the spores. The active compounds were determines as nonadecanoic acid and cis-9-nonadecenoic acid. The location of the double bond of cis-9-nonadecenoic acid was demonstrated by GC-MS analysis, based on the fragmentation pattern of the adduct prepared from the fatty acid and dimethyl disulfide.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101-0062 Japan.
Pei
P
Gao
Tomoya
T
Hirano
Zhiqing
Z
Chen
Tadashi
T
Yasuhara
Yoshihiro
Y
Nakata
Akiko
A
Sugimoto
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002244:Carbon / Q000737:chemistry; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D008517:Phytotherapy; D020940:Reishi / Q000737:chemistry*; D013172:Spores, Fungal / Q000737:chemistry
D000970:Antineoplastic Agents; D001688:Biological Products; D005227:Fatty Acids; D002244:Carbon; C517969:nonadecanoic acid