Teaching single-cell digital analysis using droplet-based microfluidics.
Microfluidics allows the manipulation of small quantities of  reagents in a high-throughput manner and is therefore highly amenable  to single cell characterization and more generally to digital analysis, with applications in fields as varied as genomics, diagnostics, directed  evolution, and drug screening. The growing place of microfluidics  in biology laboratories encouraged us to develop a teaching method  where advanced undergraduate or first-year graduate-level students  are taught to fabricate droplet-based microfluidic devices, characterize  them, and finally use them to perform a digital analysis of bacterial  samples based on a phenotypic marker.
Institut de Science et d'Ingénierie Supramoléculaires (ISIS), Université de Strasbourg, CNRS UMR 7006, 8 allée Gaspard Monge, 67083 Strasbourg Cedex, France.
Majdi
M
Najah
Andrew D
AD
Griffiths
Michael
M
Ryckelynck