Adipose tissue proteomes of intrauterine growth-restricted piglets artificially reared on a high-protein neonatal formula.
The eventuality that adipose tissues adapt to neonatal nutrition in a way that may program later adiposity or obesity in adulthood is receiving increasing attention in neonatology. This study assessed the immediate effects of a high-protein neonatal formula on proteome profiles of adipose tissues in newborn piglets with intrauterine growth restriction. Piglets (10th percentile) were fed milk replacers formulated to provide an adequate (AP) or a high (HP) protein supply from day 2 to the day prior weaning (day 28, n=5 per group). Adipocytes with small diameters were present in greater proportions in subcutaneous and perirenal adipose tissues from HP piglets compared with AP ones at this age. Two-dimensional gel electrophoresis analysis of adipose tissue depots revealed a total of 32 protein spots being up- or down-regulated (P<.10) for HP piglets compared with AP piglets; 18 of them were unambiguously identified by mass spectrometry. These proteins were notably related to signal transduction (annexin 2), redox status (peroxiredoxin 6, glutathione S-transferase omega 1, cyclophilin-A), carbohydrate metabolism (ribose-5-phosphate dehydrogenase, lactate dehydrogenase), amino acid metabolism (glutamate dehydrogenase 1) and cell cytoskeleton dynamics (dynactin and cofilin-1). Proteomic changes occurred mainly in dorsal subcutaneous adipose tissue, with the notable exception of annexin 1 involved in lipid metabolic process having a lower abundance in HP piglets for perirenal adipose tissue only. Together, modulation in those proteins could represent a novel starting point for elucidating catch-up fat growth observed in later life in growing animals having been fed HP formula.
INRA, UMR1079 Systèmes d'Elevage Nutrition Animale et Humaine, F-35590 Saint-Gilles, France.
Ousseynou
O
Sarr
Isabelle
I
Louveau
Isabelle
I
Le Huërou-Luron
Florence
F
Gondret
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D017302:Annexins / Q000378:metabolism*; D004044:Dietary Proteins / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005317:Fetal Growth Retardation; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D013552:Swine
D017302:Annexins; D004044:Dietary Proteins; D011506:Proteins; D020543:Proteome