Creation of an artificial metalloprotein with a Hoveyda-Grubbs catalyst moiety through the intrinsic inhibition mechanism of α-chymotrypsin.
An L-phenylalanyl chloromethylketone-based inhibitor equipped with a Hoveyda-Grubbs catalyst moiety was regioselectively incorporated into the cleft of α-chymotrypsin through the intrinsic inhibition mechanism of the protein to construct an artificial organometallic protein.
Graduate School of Materials Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan. tmatsuo@ms.naist.jp
Takashi
T
Matsuo
Chie
C
Imai
Takefumi
T
Yoshida
Takashi
T
Saito
Takashi
T
Hayashi
Shun
S
Hirota
D002384:Catalysis; D002918:Chymotrypsin / Q000737:chemistry*; D002942:Circular Dichroism; D008667:Metalloproteins / Q000138:chemical synthesis*; D011487:Protein Conformation; D012428:Ruthenium / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000737:chemistry
D008667:Metalloproteins; D014108:Tosylphenylalanyl Chloromethyl Ketone; D012428:Ruthenium; D002918:Chymotrypsin; C117608:alpha-chymotrypsin