The profile of β-amyloid precursor protein expression of rats induced by aluminum.
The environmental agent aluminum has been extensively investigated for a potential relationship with amyloid precursor protein (APP) expression. Despite many investigations, there is at present no definite proof from which to draw a conclusion. Since APP is an integral membrane protein expressed in different tissues and capable of fluxes across the blood-brain barrier (BBB), which may ultimately affect APP level in brain, it is necessary to assess the expression profile among vital body organs. The present study compared aluminum oxide and aluminum chloride injected rats with control rats (saline treated) to observe if aluminum affected APP expression patterns in different organs by immunohistochemistry (IHC). The expression of APP was observed in the brain of aluminum chloride treated rats and in the liver of aluminum oxide injected group. Results of double IHC staining showed that it is Kupffer cells, which are located in liver sinus and expressed APP after aluminum oxide treatment. Oxidative stress is suggested as the potential pathway that aluminum chloride exert effects in brain. These results suggest that different aluminum compounds may impact the expression of APP in brain and liver tissues. The mechanism that aluminum induced liver APP expression still needs further investigation.
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. lixiaobo1007@hotmail.com
Xiao-Bo
XB
Li
Zhi-Yuan
ZY
Zhang
Li-Hong
LH
Yin
Hermann J
HJ
Schluesener
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000537:Aluminum Oxide / Q000008:administration & dosage / Q000633:toxicity*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002712:Chlorides / Q000008:administration & dosage / Q000633:toxicity*; D002784:Cholesterol / Q000097:blood; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D017607:Aluminum Compounds; D016564:Amyloid beta-Protein Precursor; D002712:Chlorides; D004785:Environmental Pollutants; D000077410:Aluminum Chloride; D002784:Cholesterol; D000537:Aluminum Oxide