Recognize three different human telomeric G-quadruplex conformations by quinacrine.
Recognition of different human telomeric G-quadruplex structures has been a very important task for developing anti-cancer drug design. However, it also is a very challenging question since multiple conformational isomers of telomeric G-quadruplexes coexist under some conditions. Here, three different conformations including parallel, antiparallel, and mixed-type telomeric G-quadruplex structures have been well recognized by quinacrine (QNA) through monitoring its absorption, fluorescence, and fluorescence lifetime spectra. The multiple structures of H22 G-quadruplexes under physiological K(+) conditions could also be easily determined to coexist as mixed-type and antiparallel G-quadruplexes. The recognition mechanism based on the different binding affinity and binding sites has been further elucidated by association with the nuclear magnetic resonance (NMR) results.
National Laboratory for Molecular Sciences, Center for Molecular Sciences, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.
Hongxia
H
Sun
Junfeng
J
Xiang
Qian
Q
Li
Yan
Y
Liu
Lin
L
Li
Qian
Q
Shang
Guangzhi
G
Xu
Yalin
Y
Tang
D001665:Binding Sites; D015195:Drug Design; D054856:G-Quadruplexes; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D011796:Quinacrine; D019098:Telomerase / Q000037:antagonists & inhibitors / Q000378:metabolism; D016615:Telomere / Q000737:chemistry*
D019098:Telomerase; D011796:Quinacrine