Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods.
Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, associe aux Universites Paris 6 et Paris 7, CNRS UMR 8550, 24 rue Lhomond, 75005 Paris, France. gamal.rayan@lps.ens.fr
Gamal
G
Rayan
Jean-Erik
JE
Guet
Nicolas
N
Taulier
Frederic
F
Pincet
Wladimir
W
Urbach
D000818:Animals; D036681:Fluorescence Recovery After Photobleaching / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008566:Membranes / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D008962:Models, Theoretical
D046911:Macromolecular Substances; D008565:Membrane Proteins