Analysis of cellular proteome alterations in porcine alveolar macrophage cells infected with 2009 (H1N1) and classical swine H1N1 influenza viruses.
The H1N1/2009 influenza virus has the potential to cause a human pandemic, and sporadic cases of human-to-pig transmission have been reported. In this study, two influenza viruses were isolated from pigs. A phylogenetic analysis showed that the A/swine/NanChang/F9/2010(H1N1) (F9/10) strain shared a high degree of homology with the pandemic H1N1/2009 virus, and A/swine/GuangDong/34/2006 (H1N1) (34/06) strains was a classical swine influenza virus. A proteomic analysis was performed to investigate possible alterations of protein expression in porcine alveolar macrophage (PAM) cells infected by the F9/10 and 34/06 viruses over different time courses. Using 2-DE in association with MALDI-TOF MS/MS, we identified 13 up-regulated and 21 down-regulated protein spots, including cytoskeleton proteins, cellular signal transduction proteins, molecular biosynthesis proteins and heat shock proteins. The most significant changes in the infected cells were associated with molecular biosynthesis proteins and heat shock proteins. We analysed the biological characteristics of the F9/10 and 34/06 viruses in vivo and in vitro. The F9/10 virus showed greater pathogenicity than the 34/06 virus in PAM cells and mice. This study provides insights into the biologic characteristics, potential virulence alteration and cross-species transmission mechanisms of the pandemic H1N1/2009.
State Key Laboratory of Agriculture Microbiology, Huazhong Agriculture University, Wuhan 430070, PR China.
JiPing
J
Zhu
Wei
W
Zou
GuangMin
G
Jia
HongBo
H
Zhou
Yong
Y
Hu
MengYun
M
Peng
HuanChun
H
Chen
MeiLin
M
Jin
D000818:Animals; D020869:Gene Expression Profiling; D006360:Heat-Shock Proteins / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000472:pathogenicity; D016676:Macrophages, Alveolar / Q000821:virology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D058873:Pandemics; D020543:Proteome / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014774:Virulence / Q000235:genetics
D006360:Heat-Shock Proteins; D020543:Proteome