Exploring long non-coding RNAs through sequencing.
Long non-coding RNAs (lncRNAs) are emerging as an important class of regulatory transcripts that are implicated in a variety of biological functions. RNA-sequencing, along with other next-generation sequencing-based approaches, enables their study on a genome-wide scale, at maximal resolution, and across multiple conditions. This review discusses how sequencing-based studies are providing global insights into lncRNA transcription, post-transcriptional processing, expression regulation and sites of function. The next few years will deepen our insight into the overall contribution of lncRNAs to genome function and to the information flow from genotype to phenotype.
University College London, Department of Genetics, Evolution & Environment and UCL Cancer Institute, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom.
Sophie R
SR
Atkinson
Samuel
S
Marguerat
Jürg
J
Bähler
D001483:Base Sequence; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human; D006801:Humans; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods*; D014158:Transcription, Genetic
D002843:Chromatin; D022661:RNA, Untranslated