Molecular cloning, characterization, and function analysis of a mevalonate pyrophosphate decarboxylase gene from Ganoderma lucidum.
This study investigated the role of the mevalonate pyrophosphate decarboxylase gene in the triterpene biosynthetic pathway of Ganoderma lucidum. The mevalonate pyrophosphate decarboxylase gene (mvd) was isolated using a degenerate primer-PCR technique. An analysis of the Gl-mvd transcription profile revealed a positive correlation between the expression of the Gl-mvd gene and triterpene content changes in G. lucidum during development. Furthermore, a promoter deletion analysis was conducted in G. lucidum to investigate the promoter activity and the role of methyl jasmonate (MeJA) responsive elements in the mvd promoter under the MeJA elicitor. The overexpression of Gl-mvd increased triterpene accumulation compared with the wild-type strain and increased the expression of several genes involved in the triterpene biosynthetic pathway. The findings of this study suggest that mvd may play an important role in triterpene biosynthesis regulation. Moreover, there may be the interactions among the genes involved in the triterpene biosynthetic pathway in the G. lucidum. Additionally, this study provides an approach for improving triterpene content through the overexpression of a key gene.
Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu, People's Republic of China.
Liang
L
Shi
Lei
L
Qin
Yingjie
Y
Xu
Ang
A
Ren
Xing
X
Fang
Dashuai
D
Mu
Qi
Q
Tan
Mingwen
M
Zhao
D000085:Acetates / Q000494:pharmacology; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000302:isolation & purification; D004271:DNA, Fungal / Q000302:isolation & purification; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000378:metabolism; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020940:Reishi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D014170:Transformation, Genetic / Q000187:drug effects; D014315:Triterpenes / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D004271:DNA, Fungal; D054883:Oxylipins; D014315:Triterpenes; C072239:methyl jasmonate; D005966:Glucuronidase; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase