Sequence differences in the diagnostic region of the cysteine protease 8 gene of Tritrichomonas foetus parasites of cats and cattle.
In order to investigate the genetic variation between Tritrichomonas foetus from bovine and feline origins, cysteine protease 8 (CP8) coding sequence was selected as the polymorphic DNA marker. Direct sequencing of CP8 coding sequence of T. foetus from four feline isolates and two bovine isolates with polymerase chain reaction successfully revealed conserved nucleotide polymorphisms between feline and bovine isolates. These results provide useful information for CP8-based molecular differentiation of T. foetus genotypes.
Faculty of Veterinary Science, McMaster Building B14, University of Sydney, New South Wales 2006, Australia.
Zichen
Z
Sun
Colin
C
Stack
Jan
J
Šlapeta
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D057056:Cysteine Proteases / Q000235:genetics / Q000378:metabolism*; D016054:DNA, Protozoan / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011529:Protozoan Infections, Animal / Q000469:parasitology*; D016835:Tritrichomonas foetus / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D057056:Cysteine Proteases