Rapid genomic-scale analysis of Escherichia coli O104:H4 by using high-resolution alternative methods to next-generation sequencing.
Two technologies, involving DNA microarray and optical mapping, were used to quickly assess gene content and genomic architecture of recent emergent Escherichia coli O104:H4 and related strains. In real-time outbreak investigations, these technologies can provide congruent perspectives on strain, serotype, and pathotype relationships. Our data demonstrated clear discrimination between clinically, temporally, and geographically distinct O104:H4 isolates and rapid characterization of strain differences.
Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, Laurel, MD, USA.
Scott A
SA
Jackson
Michael L
ML
Kotewicz
Isha R
IR
Patel
David W
DW
Lacher
Jayanthi
J
Gangiredla
Christopher A
CA
Elkins
D002874:Chromosome Mapping / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D046228:Microarray Analysis / Q000379:methods*
D004269:DNA, Bacterial