Peptides derived from atlantic salmon skin gelatin as dipeptidyl-peptidase IV inhibitors.
The dipeptidyl-peptidase IV (DPP-IV)-inhibitory activity of peptides derived from Atlantic salmon skin gelatin hydrolyzed by alcalase (ALA), bromelain (BRO), and Flavourzyme (FLA) was determined. The FLA hydrolysate with the enzyme/substrate ratio of 6% showed the greatest DPP-IV-inhibitory activity. The hydrolysate was fractionated by ultrafiltration with 1 and 2.5 kDa cutoff membranes, and the <1 kDa fraction had the highest DPP-IV-inhibitory activity with an IC(50) value of 1.35 mg/mL. The F-1 fraction further isolated by HPLC showed the IC(50) value against DPP-IV of 57.3 μg/mL, and the peptide sequences were identified as Gly-Pro-Ala-Glu (372.4 Da) and Gly-Pro-Gly-Ala (300.4 Da). The synthetic peptides showed dose-dependent inhibition effects on DPP-IV with IC(50) values of 49.6 and 41.9 μM, respectively. The results suggest that the peptides derived from Atlantic salmon skin gelatin would be beneficial ingredients for functional foods or pharmaceuticals against type 2 diabetes.
Faculty of Land and Food Systems, The University of British Columbia, Vancouver, British Columbia, Canada.
Eunice C Y
EC
Li-Chan
Shih-Li
SL
Hunag
Chia-Ling
CL
Jao
Kit-Pan
KP
Ho
Kuo-Chiang
KC
Hsu
D000595:Amino Acid Sequence; D000818:Animals; D001963:Bromelains / Q000378:metabolism; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry*; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020317:Salmo salar; D012867:Skin / Q000737:chemistry*; D013381:Subtilisins / Q000378:metabolism
D054873:Dipeptidyl-Peptidase IV Inhibitors; D010455:Peptides; D005780:Gelatin; D001963:Bromelains; D010450:Endopeptidases; C412470:flavourzyme; D013381:Subtilisins