Profile of anti-Leishmania antibodies related to clinical picture in canine visceral leishmaniasis.
This research investigated the profile of anti-Leishmania antibodies in different clinical forms of canine visceral leishmaniasis (CVL). Naturally infected dogs were divided into two groups: subclinical dogs (SD, n=10) and clinical dogs (CD, n=68). Non-infected dogs (ND, n=7) comprised the negative control group. The humoral response was evaluated by the profile of total IgG, IgG1, IgG2, IgM, IgA and IgE, determined by ELISA. Infected animals showed increased levels of total IgG, IgA and IgE in addition to IgG1 and IgG2 in groups SD and CD, when compared with group ND. Furthermore, it was observed that IgG2 and IgM were correlated with symptomatology, while total IgG, IgG1 and IgA were negatively correlated and IgE showed no correlation. It follows that serum levels of IgG2 anti-Leishmania are correlated with typical clinical signs of disease. Furthermore the determination of specific anti-Leishmania antibodies could be an important tool in monitoring CVL clinical picture.
Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Estadual do Ceará (UECE), Avenida Paranjana, 1700, Campus do Itaperi, Serrinha, CEP 60740-000, Fortaleza, CE, Brazil. joseclaudiocarneiro@yahoo.com.br
José Cláudio Carneiro
JC
de Freitas
Belarmino Eugênio
BE
Lopes-Neto
Cyntia Rafaelle Amaral
CR
de Abreu
Wendel
W
Coura-Vital
Samuel Leôncio
SL
Braga
Alexandre Barbosa
AB
Reis
Diana Célia Sousa
DC
Nunes-Pinheiro
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004283:Dog Diseases / Q000097:blood / Q000276:immunology* / Q000473:pathology; D004285:Dogs; D007070:Immunoglobulin A / Q000097:blood / Q000145:classification; D007073:Immunoglobulin E / Q000097:blood / Q000145:classification; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D007891:Leishmania / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007073:Immunoglobulin E