Polyphenol content of plasma and litter after the oral administration of green tea and tea polyphenols in chickens.
Metabolic profiles of broiler chickens were examined after the ingestion of green tea, tea polyphenols, and (-)-epigallocatechin-3-gallate (EGCG). Solid-phase extraction of serum and litters yielded free catechins and their metabolites, which were then identified and quantified by liquid chromatography-tandem mass spectrometry. In plasma samples, (-)-gallocatechin, (+)-catechin, and EGCG were detected in the green tea group; pyrogallol acid, (epi)catechin-O-sulfate, 4'-O-methyl-(epi)gallocatechin-O-glucuronide, and (epi)catechin-3'-O-glucuronide were detected in the tea polyphenols group; and EGCG, (-)-gallocatechin gallate (GCG), and 4'-O-methyl-(epi)gallocatechin-O-glucuronides were detected in the EGCG group. In litters, gallic acid, EGCG, GCG, and ECG were detected in the green tea and tea polyphenols groups; EGCG and ECG were detected in the EGCG group. The conjugated metabolites, 4'-O-methyl-(epi)gallocatechin-O-glucuronide, (epi)catechin-3'-glucuronide, and 4'-O-methyl-(epi)catechin-O-sulfate, were identified in the green tea group; 4'-O-methyl-(epi)catechin-O-sulfate and 4'-O-methyl-(epi)gallocatechin-O-sulfate were identified in the tea polyphenols group; only 4'-O-methyl-(epi)gallocatechin-O-sulfate was detected in the EGCG group. The excretion of tea catechins was 95.8, 87.7, and 97.7% for the green tea, tea polyphenols, and EGCG groups, respectively.
Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Agriculture of China, Anhui Agricultural University, Hefei, China.
Yi-Bin
YB
Zhou
Xiao-Chun
XC
Wan
Yan-Yan
YY
Shang
Jing-Wei
JW
Hu
Lei
L
Shao
Wei
W
Chen
Da-Xiang
DX
Li
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D002645:Chickens / Q000097:blood / Q000378:metabolism*; D002853:Chromatography, Liquid; D008372:Manure / Q000032:analysis; D059808:Polyphenols / Q000008:administration & dosage* / Q000032:analysis* / Q000097:blood; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*
D008372:Manure; D059808:Polyphenols; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; C057580:gallocatechol