Regulation of focal adhesion dynamics by Wnt5a signaling.
Wnt5a is a representative ligand that activates the β-catenin-independent pathway of Wnt signaling in mammals. This pathway might be related to planar cell polarity signaling in Drosophila. Because reliable biochemical assays to measure Wnt5a pathway activity have not yet been established, we examined whether Wnt5a signaling stimulates focal adhesion turnover in migrating cells using live immunofluorescence imaging and immunocytochemical analysis. These assays demonstrated that the Wnt5a pathway cooperates with integrin signaling to regulate cell migration and adhesion through focal adhesion dynamics.
Department of Molecular Biology & Biochemistry, Graduate School of Medicine, Faculty of Medicine, Osaka University, Osaka, Japan.
Shinji
S
Matsumoto
Akira
A
Kikuchi
D000818:Animals; D002465:Cell Movement; D002470:Cell Survival; D002522:Chlorocebus aethiops; D022001:Focal Adhesions; D051157:Frizzled Receptors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D057054:Molecular Imaging; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D014162:Transfection; D014709:Vero Cells; D051153:Wnt Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000071818:Wnt-5a Protein
C489742:FZD2 protein, human; D051157:Frizzled Receptors; D019012:Integrin beta1; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein