Adsorption and protection of plasmid DNA on mesoporous silica nanoparticles modified with various amounts of organosilane.
Ordered MCM-41-type mesoporous silica nanoparticles (MSNs) with pore size of 2.6 nm were synthesized and were further modified with various amounts of 3-aminopropyltriethoxysilane (APTES), respectively, by a direct co-condensation method. These amine functionalized mesoporous silica nanoparticles (Am-MSNs) were employed to complex with plasmid DNA (pDNA) to study their adsorption and protection capacities. The results demonstrate the MSNs functionalized with aminopropyl groups present advanced adsorption capacities for pDNA immobilization. And Am-MSNs with high APTES amount lead to high amount of pDNA adsorption. Further investigation of pDNA protection shows that Am-MSNs with moderate APTES amount could completely protect pDNA from enzymatic degradation, while those with smaller and/or higher amount of APTES could partially provide protection of pDNA.
Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, PR China.
Hong
H
Yang
Kun
K
Zheng
Zuoming
Z
Zhang
Wei
W
Shi
Shubo
S
Jing
Li
L
Wang
Wei
W
Zheng
Dazhou
D
Zhao
Jianing
J
Xu
Ping
P
Zhang
D004247:DNA / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010957:Plasmids / Q000302:isolation & purification*; D016062:Porosity; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
C509968:MCM-41; D011437:Propylamines; D012821:Silanes; D012822:Silicon Dioxide; D004247:DNA; C477625:amino-propyl-triethoxysilane