Engineering of a tyrosol-producing pathway, utilizing simple sugar and the central metabolic tyrosine, in Escherichia coli.
Metabolic engineering was applied to the development of  Escherichia coli  capable of synthesizing tyrosol (2-(4-hydroxyphenyl)ethanol), an attractive phenolic compound with great industrial value, from glucose, a renewable carbon source. In this strain, tyrosine, which was supplied not only from the culture medium but also from the central metabolism, was converted into tyrosol via three steps: decarboxylation, amine oxidation, and reduction. The engineered strain synthesized both tyrosol and 4-hydroxyphenylacetate (4HPA), but disruption of the endogenous phenylacetaldehyde dehydrogenase gene shut off 4HPA production and improved the production of tyrosol as a sole product. The engineered mutant strain was capable of producing 0.5 mM tyrosol from 1% (w/v) glucose during a 48 h shake flask cultivation.
Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Yasuharu
Y
Satoh
Kenji
K
Tajima
Masanobu
M
Munekata
Jay D
JD
Keasling
Taek Soon
TS
Lee
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins; D005947:Glucose / Q000378:metabolism*; D060847:Metabolic Engineering; D010648:Phenylacetates / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*
D029968:Escherichia coli Proteins; D010648:Phenylacetates; C011867:4-hydroxyphenylethanol; C026246:4-hydroxyphenylacetate; D014443:Tyrosine; D000445:Aldehyde Oxidoreductases; C053855:phenylacetaldehyde dehydrogenase; D005947:Glucose; D010626:Phenylethyl Alcohol