Gordonia caeni sp. nov., isolated from sludge of a sewage disposal plant.
A Gram-stain-positive, strictly aerobic, short-rod-shaped, non-motile strain (designated MJ32(T)) was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ32(T). Strain MJ32(T) showed highest 16S rRNA gene sequence similarity to Gordonia hirsuta DSM 44140(T) (98.1%) and Gordonia hydrophobica DSM 44015(T) (97.0%); levels of sequence similarity to the type strains of other recognized Gordonia species were less than 97.0%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ32(T) belonged to the clade formed by members of the genus Gordonia in the family Gordoniaceae. The G+C content of the genomic DNA of strain MJ32(T) was 69.2 mol%. Chemotaxonomically, strain MJ32(T) showed features typical of the genus Gordonia. The predominant respiratory quinone was MK-9(H(2)), the mycolic acids present had C(56)-C(60) carbon atoms, and the major fatty acids were C(16:0) (34.6%), tuberculostearic acid (21.8%), C(16:1)ω7c (19.5%) and C(18:1)ω9c (12.7%). The peptidoglycan type was based on meso-2,6-diaminopimelic acid as the diagnostic diamino acid with glycolated sugars. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ32(T) is considered to represent a novel species of the genus Gordonia, for which the name Gordonia caeni sp. nov. is proposed. The type strain is MJ32(T) (=KCTC 19771(T)=JCM 16923(T)).
Research and Development Division, H-Plus Eco Ltd, BVC no. 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.|Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Sathiyaraj
S
Srinivasan
Daewoo Institute of Construction Technology, 60 Songjuk-dong, Jangan-gu, Suwon-si, Gyeonggi-do 440-210, Republic of Korea.
Giho
G
Park
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Hyejin
H
Yang
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Supyong
S
Hwang
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Yoonjung
Y
Bae
Korea Testing Certification, Chemical Industry Team, 587-10, Sinsa-Dong, Gangnam-Gu, Seoul, Republic of Korea.
Yong-An
YA
Jung
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Myung Kyum
MK
Kim
Daewoo Institute of Construction Technology, 60 Songjuk-dong, Jangan-gu, Suwon-si, Gyeonggi-do 440-210, Republic of Korea.
Myungjin
M
Lee
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D033601:Gordonia Bacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage