Role of vitamin C and E supplementation on IL-6 in response to training.
Vitamin C and E supplementation has been shown to attenuate the acute exercise-induced increase in plasma interleukin-6 (IL-6) concentration. Here, we studied the effect of antioxidant vitamins on the regulation of IL-6 expression in muscle and the circulation in response to acute exercise before and after high-intensity endurance exercise training. Twenty-one young healthy men were allocated into either a vitamin (VT; vitamin C and E, n = 11) or a placebo (PL, n = 10) group. A 1-h acute bicycling exercise trial at 65% of maximal power output was performed before and after 12 wk of progressive endurance exercise training. In response to training, the acute exercise-induced IL-6 response was attenuated in PL (P < 0.02), but not in VT (P = 0.82). However, no clear difference between groups was observed (group × training: P = 0.13). Endurance exercise training also attenuated the acute exercise-induced increase in muscle-IL-6 mRNA in both groups. Oxidative stress, assessed by plasma protein carbonyls concentration, was overall higher in the VT compared with the PL group (group effect: P < 0.005). This was accompanied by a general increase in skeletal muscle mRNA expression of antioxidative enzymes, including catalase, copper-zinc superoxide dismutase, and glutathione peroxidase 1 mRNA expression in the VT group. However, skeletal muscle protein content of catalase, copper-zinc superoxide dismutase, or glutathione peroxidase 1 was not affected by training or supplementation. In conclusion, our results indicate that, although vitamin C and E supplementation may attenuate exercise-induced increases in plasma IL-6 there is no clear additive effect when combined with endurance training.
The Centre of Inflammation and Metabolism, Department of Infectious Diseases, and Copenhagen Muscle Research Centre, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. christinayfanti@inflammation-metabolism.dk
Christina
C
Yfanti
Christian P
CP
Fischer
Søren
S
Nielsen
Thorbjörn
T
Akerström
Anders R
AR
Nielsen
Aristidis S
AS
Veskoukis
Demetrios
D
Kouretas
Jens
J
Lykkesfeldt
Henriette
H
Pilegaard
Bente K
BK
Pedersen
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000097:blood; D015992:Body Mass Index; D002374:Catalase / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010807:Physical Endurance / Q000187:drug effects* / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood; D000094765:Glutathione Peroxidase GPX1
D000975:Antioxidants; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D009124:Muscle Proteins; D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D006854:Hydrocortisone; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human