Salt stress-induced transcription of σB- and CtsR-regulated genes in persistent and non-persistent Listeria monocytogenes strains from food processing plants.
Listeria monocytogenes is a foodborne pathogen that can persist in food processing environments. Six persistent and six non-persistent strains from fish processing plants and one persistent strain from a meat plant were selected to determine if expression of genes in the regulons of two stress response regulators, σ(B) and CtsR, under salt stress conditions is associated with the ability of L. monocytogenes to persist in food processing environments. Subtype data were also used to categorize the strains into genetic lineages I or II. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was used to measure transcript levels for two σ(B)-regulated genes, inlA and gadD3, and two CtsR-regulated genes, lmo1138 and clpB, before and after (t=10 min) salt shock (i.e., exposure of exponential phase cells to BHI+6% NaCl for 10 min at 37°C). Exposure to salt stress induced higher transcript levels relative to levels under non-stress conditions for all four stress and virulence genes across all wildtype strains tested. Analysis of variance (ANOVA) of induction data revealed that transcript levels for one gene (clpB) were induced at significantly higher levels in non-persistent strains compared to persistent strains (p=0.020; two-way ANOVA). Significantly higher transcript levels of gadD3 (p=0.024; two-way ANOVA) and clpB (p=0.053; two-way ANOVA) were observed after salt shock in lineage I strains compared to lineage II strains. No clear association between stress gene transcript levels and persistence was detected. Our data are consistent with an emerging model that proposes that establishment of L. monocytogenes persistence in a specific environment occurs as a random, stochastic event, rather than as a consequence of specific bacterial strain characteristics.
Department of Food Science, Cornell University, Ithaca, New York 14853, USA.
Daina L
DL
Ringus
Reid A
RA
Ivy
Martin
M
Wiedmann
Kathryn J
KJ
Boor
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D049071:Endopeptidase Clp / Q000235:genetics / Q000378:metabolism; D005516:Food Microbiology; D005525:Food-Processing Industry; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D050296:Microbial Viability; D008954:Models, Biological; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012462:Saline Solution, Hypertonic; D055049:Salt Tolerance; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D013269:Stochastic Processes; D013312:Stress, Physiological; D015854:Up-Regulation
D001426:Bacterial Proteins; C118338:CtsR protein, bacteria; D018832:Molecular Chaperones; D012333:RNA, Messenger; D012097:Repressor Proteins; D012462:Saline Solution, Hypertonic; C480945:SigB protein, Listeria monocytogenes; D012808:Sigma Factor; C069761:internalin protein, Bacteria; D049071:Endopeptidase Clp; D005968:Glutamate Decarboxylase