Localization of linear B-cell epitopes on goose parvovirus structural protein.
Goose parvovirus (GPV), a small non-enveloped ssDNA virus, can cause Derzsy's disease, a highly contagious and lethal disease in goslings and muscovy ducklings, leading to a huge economic loss. However, little is known about the localization of B-cell epitopes on GPV structural protein. To address the issue, the structural protein of GPV was dissected into sets of partially overlapping fragments and expressed in Escherichia coli. Then Western blot reactivity of these glutathione S-transferase (GST) fusion short peptides to viral infected sera was surveyed. The results showed linear immunodominant epitopes, which were found in seven fragments covering amino acid residues 35-71, 123-198, 423-444, 474-491, 531-566, 616-669, 678-732. Our findings may provide the basis for the development of immunity-based prophylactic, therapeutic, and diagnostic clinical techniques for Derzsy's disease.
College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, PR China.
Tian-fei
TF
Yu
Bo
B
Ma
Ming-chun
MC
Gao
Jun-wei
JW
Wang
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D018604:Epitope Mapping / Q000662:veterinary; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005777:Geese / Q000276:immunology / Q000821:virology*; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000276:immunology* / Q000648:ultrastructure; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D016415:Sequence Alignment / Q000662:veterinary; D015678:Viral Structural Proteins / Q000276:immunology* / Q000648:ultrastructure
D018985:Epitopes, B-Lymphocyte; D015678:Viral Structural Proteins