Efficient formation of cell spheroids using polymer nanofibers.
Spheroid culture has been used for suspension cultures of anchorage-dependent cells. In this study, we developed a new method for the suspension cultures of anchorage-dependent animal cells using polymer nanofibers. Poly(lactic-co-glycolic acid) nanofibers (785 nm in average fiber-diameter, 88 μm in average fiber-length) fabricated by the electrospinning method were added to each suspension culture of human embryonic kidney 293 cells and human dermal fibroblasts. As compared to no addition of nanofibers to the suspension cultures, nanofibers enhanced cell spheroid formation, thereby reducing cell death resulting from a lack of cell adhesion. Efficient formation of spheroids in the presence of polymer nanofibers may be useful for the suspension cultures of anchorage-dependent cells.
School of Chemical and Biological Engineering, Seoul National University, 56-1, Silim-dong, Gwanak-Gu, Seoul, 151-744, Republic of Korea. monce@snu.ac.kr
Jung-Youn
JY
Shin
Jooyeon
J
Park
Hyeon-Ki
HK
Jang
Tae-Jin
TJ
Lee
Wan-Geun
WG
La
Suk Ho
SH
Bhang
Il Keun
IK
Kwon
Oh Hyeong
OH
Kwon
Byung-Soo
BS
Kim
D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D057139:Nanofibers; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D018874:Spheroids, Cellular / Q000187:drug effects*
D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid