Antibacterial potential of sponge endosymbiont marine Enterobacter sp at Kavaratti Island, Lakshadweep archipelago.
OBJECTIVE
To isolate antibacterial potential of sponge endosymbiotic bacteria from marine sponges at Lakshadweep archipelago. Also to identify the potent bacteria by 16s rDNA sequencing and determine the antibacterial activity against clinical pathogens by MIC.


METHODS
Sponge samples was collected from sub-tidal habitats at Kavaratti Island and identified. The endosymbiotic bacteria were isolated and selected potential bacteria which show antibacterial activity in preliminary screening against clinical pathogens Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Salmonella typhi (S. typhi), Klebsiella pneumoniea (K. pneumoniea) and Streptococcus sp. by disc diffusion assay. The crude extracts of potential bacteria LB3 was tested against clinical pathogens by MIC. The LB3 strain was identified by 16s rDNA sequencing, 1 111 bp was submitted in NCBI (HQ589912) and constructed phylogenetic tree.


RESULTS
Sponge sample was identified as Dysidea granulosa (D. granulosa) and potential bacteria LB3 identified as Enterobacter sp TTAG. Preliminary screening of sponge isolates against clinical pathogens, LB3 strain was selected as potential producer of secondary metabolites and crude extract was implies on MIC of LB3 have confirmed with lowest concentration of 5.0 mg/mL in broth medium influence of crude extract on growth inhibitory activity after 5 h of incubation period and completed the inhibitory activity at 15 h.


CONCLUSIONS
The present study concluded that phylogenetic analysis of endosymbiotic bacteria Enterobacter sp from sponge D. granulosa of Lakshadweep islands showed significant antibacterial activity against clinical bacterial pathogens.
Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu, India; CMLRE Field Research Station, Agatti Island, Lakshadweep, India.
Mohan
M
Gopi
Subramanian
S
Kumaran
Thipparamalai Thangappanpillai Ajith
TT
Kumar
Balaraman
B
Deivasigamani
Kumarappan
K
Alagappan
Srinivasan Guru
SG
Prasad
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000737:chemistry; D047088:Dysidea / Q000737:chemistry* / Q000382:microbiology; D004754:Enterobacter / Q000737:chemistry*; D004755:Enterobacteriaceae / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects; D007194:India; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008386:Marine Biology; D010802:Phylogeny; D012485:Salmonella typhi / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents