Microfluidic dual emitter electrospray ionization source for accurate mass measurements.
A glass microfluidic device with two independent electrospray ionization (ESI) emitters has been designed to sequentially generate ions from different solutions for mass analysis. Rapid modulation between the emitters is accomplished by turning on and off the voltage that simultaneously generates the fluid flow rate and ESI potential. The time required to switch between the two electrospray signals is less than 70 ms. Using the second emitter to introduce a reference compound for internal calibration, accurate mass measurements (less than 3 ppm mass error) were obtained with a time-of-flight mass spectrometer.
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Andrew G
AG
Chambers
J Michael
JM
Ramsey
D004743:Enkephalin, Leucine / Q000032:analysis; D046210:Microfluidic Analytical Techniques; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*; D013997:Time Factors
D004743:Enkephalin, Leucine