Hepatitis B virus X protein promotes the growth of hepatocellular carcinoma             by modulation of the Notch signaling pathway.
Hepatitis B virus X protein (HBx) plays a crucial role in the development             of hepatocellular carcinoma (HCC), however, little is known about the mechanism.             Here, we investigated the relationship between HBx and Notch signaling in HepG2             cells after they were transfected with the HBx gene. It was found that HBx upregulated             the expression of Notch-1, Jagged-1 and Hes-1 at the transcriptional level by             binding to the Notch-1 intracellular domain, which is congruent with the observations             of enhanced malignant biological activities of HBx-transfected HepG2 cells compared             with normal HepG2 cells. However, while Notch signaling was blocked, the HBx-induced             abnormalities were partially reversed. These findings suggest that HBx may promote             the progression of HCC via the activated Notch pathway.
Department of Gastroenterology and Hepatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
Fan
F
Wang
Haiyan
H
Zhou
Yuzhen
Y
Yang
Xiumei
X
Xia
Qian
Q
Sun
Jin
J
Luo
Bin
B
Cheng
D017209:Apoptosis; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology; D056945:Hep G2 Cells; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D000072100:Jagged-1 Protein; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism; D051881:Receptor, Notch1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D000072056:Transcription Factor HES-1; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D004151:Dipeptides; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051881:Receptor, Notch1; D000072099:Serrate-Jagged Proteins; D015534:Trans-Activators; D000072056:Transcription Factor HES-1; D054334:Viral Regulatory and Accessory Proteins; C000606273:JAG1 protein, human; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; C497761:NOTCH1 protein, human; C057628:hepatitis B virus X protein; C078493:HES1 protein, human