Antioxidant activity of protein hydrolysates from aqueous extract of velvet antler (Cervus elaphus) as influenced by molecular weight and enzymes.
The crude protein hydrolysates from aqueous extract of velvet antler (AEVA) were prepared by simulated gastrointestinal digestion (SGI, pepsin-pancreatin) using pancreatin-pepsin, alcalase and neutrase. The resulting hydrolysates were separated by sequential ultrafiltration into four fractions. The antioxidant activities of peptide fractions were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and Fe(2+)-chelating assays. Results showed that the hydrolysate prepared by SGI had a low degree of hydrolysis, which was significantly improved with altered proteases, such as pancreatin-pepsin and alcalase. Antioxidant activities of peptide fractions varied with molecular weight (MW) and the enzyme used. Generally, low-MW peptide fractions had higher ABTS radical scavenging activity and Fe(2+)-chelating ability, and high-MW peptide fractions were more effective in DPPH radical scavenging activity and reducing power.
Beijing Higher Institution Engineering Research Center of Food Additives and Ingredient, Beijing Technology and Business University, Beijing 100048, China.
Lei
L
Zhao
Yang-Chao
YC
Luo
Cheng-Tao
CT
Wang
Bao-Ping
BP
Ji
D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D000999:Antlers / Q000737:chemistry*; D001188:Artiodactyla; D001688:Biological Products / Q000737:chemistry*; D006867:Hydrolases; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*
D000975:Antioxidants; D001688:Biological Products; D010455:Peptides; D006867:Hydrolases