Essential roles of zebrafish bmp2a, fgf10, and fgf24 in the specification of the ventral pancreas.
In vertebrates, pancreas and liver arise from bipotential progenitors located in the embryonic gut endoderm. Bone morphogenic protein (BMP) and fibroblast growth factor (FGF) signaling pathways have been shown to induce hepatic specification while repressing pancreatic fate. Here we show that BMP and FGF factors also play crucial function, at slightly later stages, in the specification of the ventral pancreas. By analyzing the pancreatic markers pdx1, ptf1a, and hlxb9la in different zebrafish models of BMP loss of function, we demonstrate that the BMP pathway is required between 20 and 24 h postfertilization to specify the ventral pancreatic bud. Knockdown experiments show that bmp2a, expressed in the lateral plate mesoderm at these stages, is essential for ventral pancreas specification. Bmp2a action is not restricted to the pancreatic domain and is also required for the proper expression of hepatic markers. By contrast, through the analysis of fgf10(-/-); fgf24(-/-) embryos, we reveal the specific role of these two FGF ligands in the induction of the ventral pancreas and in the repression of the hepatic fate. These mutants display ventral pancreas agenesis and ectopic masses of hepatocytes. Overall, these data highlight the dynamic role of BMP and FGF in the patterning of the hepatopancreatic region.
Unit of Molecular Biology and Genetic Engineering, GIGA-Research, University of Liège, B-4000 Sart-Tilman, Belgium, Germany.
François
F
Naye
Marianne L
ML
Voz
Nathalie
N
Detry
Matthias
M
Hammerschmidt
Bernard
B
Peers
Isabelle
I
Manfroid
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000502:physiology*; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000502:physiology*; D055785:Gene Knockdown Techniques; D008099:Liver / Q000196:embryology; D010179:Pancreas / Q000196:embryology*; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
D055396:Bone Morphogenetic Protein 2; D051526:Fibroblast Growth Factor 10; D029961:Zebrafish Proteins; C405936:bmp2b protein, zebrafish; C520413:fgf10a protein, zebrafish; C479567:fibroblast growth factor 24, zebrafish; D005346:Fibroblast Growth Factors