Cohnella soli sp. nov. and Cohnella suwonensis sp. nov. Isolated from soil samples in Korea.
Two bacterial isolates from soil samples taken in Korea, strains YM2-7(T) and WD2-19(T), were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-positive, motile with peritrichous flagella, and rod-shaped. Both strains formed ellipsoidal bulging positioned subterminal spores. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the Firmicutes. The 16S rRNA gene sequence similarity between YM2-7(T) and WD2-19(T) was 96.5%. Strains YM2-7(T) and WD2-19(T) showed 16S rRNA gene sequence similarities of 93.0-96.5% to type strains of recognized Cohnella species. The G+C contents of the DNA of strains YM2-7(T) and WD2-19(T) were 52.2 and 55.6 mol%, respectively. The major fatty acids of strains YM2-7(T) and WD2-19(T) were anteiso-C15:0 (44.4%), C16:0 (19.2%), and iso-C16:0 (16.8%) and anteiso-C15:0 (46.5%), iso-C16:0 (21.8%), and C16:0 (11.2%), respectively. Both strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone. Both strains had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysophosphatidylglycerol as the major polar lipids. Comparative analysis of phenotypic and phylogenetic traits indicated that strains YM2-7(T) and WD2-19(T) represented two novel species of the genus Cohnella. The names Cohnella soli sp. nov. (type strain YM2-7(T) =KACC 13346(T) =NBRC 106486(T)), and Cohnella suwonensis sp. nov. (type strain WD2-19T =KACC 13347(T) =NBRC 106485(T)) are proposed for these organisms.
Korean Agricultural Culture Collection (KACC), Agricultural Microbiology Team, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon 441-853, Republic of Korea.
Soo-Jin
SJ
Kim
Hang-Yeon
HY
Weon
Yi-Seul
YS
Kim
Soon-Wo
SW
Kwon
D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S