C. elegans cell cycle analysis.
Caenorhabditis elegans is an important system for the study of cell cycle regulation in the context of animal development. One of the most powerful features of C. elegans is the invariant cell lineage in which somatic cells initiate cell division at specific times within the developmental program. The cell lineage is fully known and provides the foundation for the analysis of cell cycle progression at single-cell resolution in a multicellular organism. In this chapter, we describe the different types of cell cycles observed in C. elegans, and provide methods and reagents for the analysis of cell cycle progression as well as specific cell cycle phases. We also provide strategies for the analysis and proper interpretation of cell cycle and checkpoint mutants.
Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
Sander
S
van den Heuvel
Edward T
ET
Kipreos
D000818:Animals; D000909:Antibodies, Helminth; D015415:Biomarkers / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D019070:Cell Lineage; D018509:DNA, Helminth / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis / Q000235:genetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D059010:Single-Cell Analysis; D013696:Temperature; D013997:Time Factors
D000909:Antibodies, Helminth; D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D018509:DNA, Helminth