5-amino-2-mercapto-1,3,4-thidiazole modified single-use sensors for electrochemical DNA analysis.
In this study, single-use graphite electrodes modified with 5-amino-2-mercapto-1,3,4-thidiazole (AMT) were fabricated for electrochemical monitoring of DNA. The surfaces of AMT modified pencil graphite electrodes (PGEs) were characterized by scanning electron microscopy (SEM). Electrochemical behaviors of these electrodes were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The effects of pretreatment of PGE and immobilization time and concentration of AMT were studied to facilitate an effective immobilization on PGE surface. The efficiency of immobilization is verified by measuring the oxidation signals of either AMT itself or adenine base of DNA, immobilized onto AMT modified electrodes.
Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, Izmir, Turkey.
Mihrican
M
Muti
Arzum
A
Erdem
A Ersin
AE
Karagözler
Mert
M
Soysal
D000225:Adenine / Q000737:chemistry; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009841:Oligonucleotides / Q000032:analysis*; D010084:Oxidation-Reduction; D012996:Solutions; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013830:Thiadiazoles / Q000737:chemistry*
D009841:Oligonucleotides; D012996:Solutions; D013438:Sulfhydryl Compounds; D013830:Thiadiazoles; D006108:Graphite; D004247:DNA; D000225:Adenine