Immunoinhibitory effect of teuclatriol a guaiane sesquiterpene from Salvia mirzayanii.
BACKGROUND
Salvia mirzayanii, a native plant to Iran, is shown to have immunomodulatory effects on lymphocyte proliferation.


OBJECTIVE
To identify the bioactive immunomodulatory compound(s) present in S. mirzayanii.


METHODS
The crude extract was fractionated to five fractions in two steps using different solvents. The fractions were subjected to bioassay-guided fractionation. All the fractions were tested for bioactivity on human activated-peripheral blood lymphocytes (PBLs) using cell proliferation assay.


RESULTS
The methanol fraction (Fr. M) showed the highest inhibitory effect on PBLs compared to other fractions. Fr. M was applied on a gravity column chromatography for further fractionation. Resultant fractions, demonstrated inhibitory effects at higher concentrations. Fr. 4 with an 18.9 ± 0.2% inhibitory activity at 200 µg/ml and with the highest quantity was applied on preparative TLC plates for further purification. The final purified compound was identified as teuclatriol, a guaiane sesquiterpene, by NMR analysis. This compound showed a significant anti-proliferative effect on human activated-peripheral blood lymphocytes (IC50, 72.8 ± 5.4 µg/ml).


CONCLUSIONS
Teuclatriol was found to be one of the compounds responsible for the immunoinhibitory effect of Salvia mirzayanii. We suggest further studies on teuclatriol, exploring its mechanism of action as an immunomodulatory compound.
Pharmaceutical Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran, e-mail: ramezanim@mums.ac.ir.
Akram
A
Ziaei
Zahra
Z
Amirghofran
Josef
J
Zapp
Mohammad
M
Ramezani
D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005591:Chemical Fractionation; D003516:Cycloparaffins / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D027544:Salvia / Q000276:immunology*; D045789:Sesquiterpenes, Guaiane
D003516:Cycloparaffins; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D045789:Sesquiterpenes, Guaiane; C546807:teucladiol