A DNA aptamer sensor for 8-oxo-7,8-dihydroguanine.
Abasic site-containing DNA duplex is a versatile structural motif that can be used for the design of purine aptamers and sensors. In this study, several modifications were introduced to the abasic site to explore possible specific binding of free 8-oxoG, a product of DNA base excision repair. The nucleoside opposite the abasic site was replaced by pyrrolo-dC as a reporter group. Binding of 8-oxoG quenched pyrrolo-dC fluorescence by as much as 70%. In contrast, adenine, guanine, thymine, and cytosine showed only minimal fluorescence quenching effect. The best aptamer binds 8-oxoG with a dissociation constant of 5.5±0.8μM. This sensor can be used to accurately measure 8-oxoG concentrations in the presence of guanine.
Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA.
Jyoti
J
Roy
Payal
P
Chirania
Sanniv
S
Ganguly
Haidong
H
Huang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide; C024829:8-hydroxyguanine; D006147:Guanine