Sildenafil accelerates anterior chamber refilling after paracentesis in sheep and rabbits.
OBJECTIVE
Sildenafil increases ocular blood flow. Thus, the authors investigated if it also increases anterior chamber (AC) refilling after paracentesis.


METHODS
Corriedale sheep and albino rabbits were used as animal models. Intraocular pressure (IOP) was measured, paracentesis performed on one eye, and AC refilling followed by observation using oblique illumination. IOP measurements continued as the AC formed. After IOP stabilization, sildenafil (100 mg) was orally administered. Forty to 60 minutes later, AH was withdrawn from the contralateral eye. The point at which IOP recovered was used to determine refilling time. Paracentesis volumes were either 60, 120, or 300 μL in sheep, and 50 or 100 μL in rabbits.


RESULTS
IOP recovered in approximately 49, 56, and 50 minutes after the 60, 120, and 300 μL withdrawals in sheep. The refilling times of the contralateral eye after sildenafil ingestion were approximately 19, 26, and 37 minutes for the respective AH withdrawals. With rabbits, IOP recovered in approximately 13 minutes after the 50 and 100 μL AH withdrawals. After sildenafil, the IOP recovery times of the fellow eye were approximately 6 minutes. AH refilling rates were estimated by dividing the paracentesis volume by IOP recovery time. After sildenafil, such rates were larger than the AH formation rate attributed to secretion by the ciliary epithelium.


CONCLUSIONS
Sildenafil accelerates the rate of AC refilling and might have beneficial utility as an agent enhancing fluid entry into the AC of patients who experienced AH loss during eye surgery, as well as in some cases of ocular hypotony.
Departamento de Oftalmología, Universidad Nacional del Nordeste, Corrientes, Argentina.
Rosana
R
Gerometta
Lawrence J
LJ
Alvarez
Oscar A
OA
Candia
D000818:Animals; D000867:Anterior Chamber / Q000503:physiopathology* / Q000601:surgery; D001082:Aqueous Humor / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007429:Intraocular Pressure / Q000187:drug effects*; D008297:Male; D015814:Ocular Hypotension / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D019152:Paracentesis; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D011817:Rabbits; D020127:Recovery of Function / Q000187:drug effects*; D012039:Regional Blood Flow / Q000187:drug effects; D012756:Sheep; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology*; D014065:Tonometry, Ocular; D014665:Vasodilator Agents / Q000494:pharmacology
D010879:Piperazines; D011687:Purines; D013450:Sulfones; D014665:Vasodilator Agents; D000068677:Sildenafil Citrate