iPSCs are transcriptionally and post-transcriptionally indistinguishable from fESCs.
Induced pluripotent stem cells (iPSCs) are generated by reprogramming mouse or human somatic cells to a pluripotent state by introducing key transcription factors and have great therapeutic potential. It has been illustrated that the transcriptional and post-transcriptional profiles of nuclear-transferred embryonic stem cells (ntESCs) is identical to those of embryonic stem cells derived from fertilized blastocysts (fESCs). Although iPSCs seem to be indistinguishable from fESCs, the degree of transcriptomic and proteomic similarity among iPSCs, ntESCs, and fESCs has not yet been elucidated completely. To investigate whether iPSCs and fESCs have similar therapeutic potential, we compared mRNA and protein pro?les of mouse iPSC, ntESCs, and matching fESCs lines using microarray technology, iTRAQ method, and bioinformatic analyses. Real-time PCR, two-dimensional LC, and MS/MS analyses were further conducted to study the expression of speci?c transcripts and identify and quantitate 929 proteins. Our results demonstrate that, like ntESCs, the iPSC and matching fESCs lines have very similar transcriptional and protein expression profiles. This is consistent with their similar developmental potential.
Department of Otolaryngology, Affiliated Eye and ENT hospital of Fudan University, Shanghai 200031, China.
Yun-Feng
YF
Wang
Jian
J
Li
Ying-Zi
YZ
He
Hui-Qian
HQ
Yu
Yang
Y
Li
Xiao-Dong
XD
Gu
Wen
W
Li
Hua-wei
HW
Li
D000818:Animals; D002852:Chromatography, Ion Exchange; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D053719:Tandem Mass Spectrometry; D014158:Transcription, Genetic