Human complement factor 3 polymorphism determination by capillary electrophoresis of serum.
Variability of complement factor 3 (C3) mobility in serum protein electrophoresis was investigated. We found that the migration time of C3 can be reproducibly determined (beween-run CV=0.76%) using clinical capillary electrophoresis (CE) equipment (the Capillarys™ 2 system, Sebia). Moreover, we found a significant difference (p<0.001) in migration times of the major C3 phenotypes FF (fast-fast), FS (fast-slow) and SS (slow-slow). Glycosylation did not significantly affect test results. This is the first report on the migration time of C3 phenotypes on a clinical CE instrument. The presented method allows faster data than agarose-electrophoresis or genotyping. Moreover, reference ranges for serum C3 concentration depend on C3 phenotype, which allows a better tailored clinical interpretation of C3 concentrations.
Department of Clinical Chemistry, Microbiology and Immunology, Ghent University, Ghent, Belgium.
Evy
E
De Witte
Marijn M
MM
Speeckaert
Lot
L
Van De Moortel
Elke
E
Lecocq
Joris R
JR
Delanghe
D000328:Adult; D000704:Analysis of Variance; D001798:Blood Proteins / Q000737:chemistry*; D015153:Blotting, Western; D003176:Complement C3 / Q000737:chemistry / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011110:Polymorphism, Genetic
D001798:Blood Proteins; D003176:Complement C3