ZBTB1 is a determinant of lymphoid development.
In this study, we describe a chemically induced mouse mutation that caused a complete and cell-intrinsic T cell deficiency. Development of other lymphoid lineages was also partially impaired and was severely compromised under competitive conditions. Positional cloning, retroviral transduction, and a somatic reversion event revealed that the causative mutation lay within Zbtb1 (zinc finger and BTB domain containing 1), a gene conserved throughout vertebrate evolution. Our data establish ZBTB1 as a critical determinant of T cell development and lymphopoiesis in general, most likely by acting as a transcriptional regulator.
Department of Genetics, The Scripps Research Institute, La Jolla, CA 92037, USA.
Owen M
OM
Siggs
Xiaohong
X
Li
Yu
Y
Xia
Bruce
B
Beutler
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008231:Lymphopenia / Q000235:genetics; D038041:Lymphopoiesis / Q000235:genetics; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation; D022423:Myeloid Cells; D012097:Repressor Proteins / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics
D012097:Repressor Proteins; C571129:ZBTB1 protein, mouse