Inhibitory effects of water-soluble low-molecular-weight β-(1,3-1,6) D-glucan isolated from Aureobasidium pullulans 1A1 strain black yeast on mast cell degranulation and passive cutaneous anaphylaxis.
We investigated the effects of water-soluble low-molecular-weight β-(1,3-1,6) D-glucan isolated from Aureobasidium pullulans 1A1 strain black yeast (LMW-β-glucan) on mast cell-mediated anaphylactic reactions. Although it is known that LMW-β-glucan has anti-tumor, anti-metastatic and anti-stress effects, the roles of LMW-β-glucan in immediate-type allergic reactions have not been fully investigated. We examined whether LMW-β-glucan could inhibit mast cell degranulation and passive cutaneous anaphylaxis (PCA). LMW-β-glucan dose-dependently inhibited the degranulation of both rat basophilic leukemia (RBL-2H3) and cultured mast cells (CMCs) activated by calcium ionophore A23187 or IgE. However, LMW-β-glucan had no cytotoxicity towards RBL-2H3 cells and CMCs. Furthermore, orally administered LMW-β-glucan inhibited the IgE-induced PCA reaction in mice. These results show LMW-β-glucan to be a possible compound for the effective therapeutic treatment of allergic diseases.
Department of Food and Nutrition, Faculty of Human Life Sciences, Senri Kinran University, Suita, Osaka, Japan.
Harumi
H
Sato
Yuko
Y
Kobayashi
Atsushi
A
Hattori
Toshio
T
Suzuki
Munekazu
M
Shigekawa
Tomoko
T
Jippo
D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D002199:Capillary Permeability / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005936:Glucans / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D012995:Solubility; D014867:Water / Q000737:chemistry*
D018926:Anti-Allergic Agents; D005936:Glucans; C033671:epiglucan; D014867:Water; D007073:Immunoglobulin E