[The role of metabolic activation of promutagens in the genome destabilization under pheromonal stress in the house mouse (Mus musculus)].
The hypothesis on a relationship between the high frequency of mitotic disturbances in bone marrow cells and the change in the activity of the S9 liver fraction containing promutagen-activating enzymes under olfactory stress in the house mouse Mus musculus has been tested. For this purpose, the effect of the pheromone 2,5-dimethylpyrazine on the frequency of mitotic disturbances in mouse bone marrow cells has been measured by the anaphase-telophase assay. The Ames test using Salmonella typhimurium has been employed to compare the capacities of the S9 liver fractions from stressed and intact mice for activating the promutagen 2-aminofluorene. It has been demonstrated that the increased frequency of mitotic disturbances in bone marrow cells induced by the pheromonal stressor in male house mice is accompanied by an increased promutagen-activating capacity of the S9 liver fraction. The model system used in the study allowed the genetic consequences of the exposure to the olfactory stressor to be estimated and the possible mechanisms of genome destabilization to be assumed.
A S
AS
Zhuk
E I
EI
Stepchenkova
A V
AV
Dukel'skaia
E V
EV
Daev
S G
SG
Inge-Vechtomov
D000818:Animals; D001711:Biotransformation; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D043171:Chromosomal Instability; D005449:Fluorenes / Q000378:metabolism / Q000633:toxicity; D042822:Genomic Instability; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008938:Mitosis / Q000187:drug effects / Q000235:genetics*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity; D010675:Pheromones / Q000378:metabolism* / Q000633:toxicity; D011719:Pyrazines / Q000378:metabolism* / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
D005449:Fluorenes; D009153:Mutagens; D010675:Pheromones; D011719:Pyrazines; C012177:2-aminofluorene; C075524:2,5-dimethylpyrazine