Beet soil-borne mosaic virus RNA-4 encodes a 32 kDa protein involved in symptom expression and in virus transmission through Polymyxa betae.
Beet soil-borne mosaic virus (BSBMV), like Beet necrotic yellow vein virus (BNYVV), is a member of the Benyvirus genus and both are transmitted by Polymyxa betae. Both viruses possess a similar genomic organization: RNA-1 and -2 are essential for infection and replication while RNA-3 and -4 play important roles in disease development and vector-mediated infection in sugar beet roots. We characterized a new species of BSBMV RNA-4 that encodes a 32 kDa protein and a chimeric form of BSBMV RNA-3 and -4. We demonstrated that BSBMV RNA-4 can be amplified by BNYVV RNA-1 and -2 in planta, is involved in symptoms expression on Chenopodium quinoa plants and can also complement BNYVV RNA-4 for virus transmission through its vector P. betae in Beta vulgaris plants. Using replicon-mediated expression, we demonstrate for the first time that a correct expression of RNAs-4 encoded proteins is essential for benyvirus transmission.
DiSTA - Plant Pathology, University of Bologna, Viale G. Fanin 40, 40127 Bologna, Italy.
Massimiliano
M
D'Alonzo
Alice
A
Delbianco
Chiara
C
Lanzoni
Concepcion Rubies
CR
Autonell
David
D
Gilmer
Claudio
C
Ratti
D027461:Beta vulgaris / Q000821:virology*; D009029:Mosaic Viruses / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D056909:Plasmodiophorida / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics* / Q000378:metabolism; D012988:Soil Microbiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D014764:Viral Proteins