Morphological, biological and molecular characterization of three strains of Trypanosoma cruzi Chagas, 1909 (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sordida (Stal) 1859 (Hemiptera, Reduviidae) and a domestic cat.
A study was conducted of the biological, morphological and molecular characters of 3 strains of Trypanosoma cruzi (SI(5), SI(8) and SIGR(3)) isolated from specimens of Triatoma sordida collected in Santo Inácio and a domestic cat. In order to carry out the study, the following parameters were evaluated: pre-patent period, parasitaemia curves, morphology of the parasites, mortality rates, histopathological lesions and molecular typing. The strains presented variable pre-patent periods, low parasitaemia and no animal mortality. The morphological study of trypomastigotes showed a predominance of intermediate-width and short-length forms, as well as low nuclear index. Epimastigotes presented a low nuclear index, intermediate-width forms in strains SI(5) and SI(8), and large-width forms in SIGR(3). A shorter length could be noted in strains SI(8) and SIGR3, whereas SI(5) displayed an intermediate length. The histopathological study did not detect amastigote nests in tissues. The amplification of the divergent domain of 24Sα rRNA, HSP60 and GPI genes of strains SI(5), SI(8) and SIGR(3) classified the 3 strains into Group II. Biological parameters made it possible to classify the strains isolated in Santo Inácio (BA) into Biodeme III, Zymodeme 1 and Group II of T. cruzi.
Instituto de Biologia Universidade Estadual de Campinas, Unicamp - Cidade Universitária Zeferino Vaz, Rua Monteiro Lobato, 255 Campinas SP, CEP 13083-862, Brasil.
Aline
A
Rimoldi
Renata
R
Tomé Alves
Daniela Luz
DL
Ambrósio
Maria Zenaide Tita
MZ
Fernandes
Isabel
I
Martinez
Renato Freitas
RF
De Araújo
Regina Maria Barreto
RM
Cicarelli
João Aristeu
JA
Da Rosa
D000818:Animals; D002371:Cat Diseases / Q000469:parasitology*; D002415:Cats; D014355:Chagas Disease / Q000453:epidemiology / Q000401:mortality / Q000469:parasitology* / Q000473:pathology; D005838:Genotype; D051379:Mice; D008807:Mice, Inbred BALB C; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000145:classification / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*