Identification of two novel HLA-A*02 variants, A*02:319 and A*02:01:64, in two Taiwanese marrow stem cell donors by sequence-based typing.
We report here two novel variants of HLA-A*02 allele, A*02:319 and A*02:01:64, discovered in two Taiwanese unrelated volunteer bone marrow donors by sequence-based typing (SBT) method. The DNA sequence of A*02:319 is identical to A*02:07 in exons 2 and 3 but varies with one nucleotide at codon 9 (TTC->TCC). The variation caused one amino acid substitution at residue 9 (F->S). On the other hand, the DNA sequence of A*02:01:64 is identical to the sequence of A*02:01:01:01 in exons 2 and 3 except a silent mutation at codon 114 (CAC->CAT). The probable HLA-A, HLA-B and HLA-DRB1 haplotypes in association with A*02:319 and A*02:01:64 were deduced as A*02:319-B*46:01-DRB1*04 and A*02:01:64-B*38:02-DRB1*16:02, respectively.
Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
K L
KL
Yang
S K
SK
Lee
S Y
SY
Yang
R H
RH
Kao
C L
CL
Lin
P Y
PY
Lin
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D001782:Blood Donors; D001854:Bone Marrow Cells / Q000166:cytology; D014644:Genetic Variation; D060005:Genotyping Techniques / Q000379:methods*; D015789:HLA-A2 Antigen / Q000235:genetics*; D006239:Haplotypes; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D011110:Polymorphism, Genetic; D013624:Taiwan
C524866:HLA-A*02 antigen; D015789:HLA-A2 Antigen