The application of high throughput siRNA screening technology to study host-pathogen interactions.
Recent advances in high throughput screening technologies have accelerated the identification and characterization of potential factors involved in host-virus interactions, facilitating early detection and diagnosis of diseases, as well as providing promising drug targets. The last decade has seen a plethora of successful examples of high throughput screening approaches, especially siRNA screening. With support from protein interaction studies, mRNA expression profiling, and bioinformatics, siRNA screening has also been successfully utilized to identify host factors required for a number of viruses including HIV, West Nile virus and H1N1 virus. Such studies have raised the awareness of virologists, and have opened a new chapter of global analysis of host-pathogen interactions. However, to play a more defining role in prognostics, diagnostics and therapeutics for virus diseases, acknowledged drawbacks, including false positives and negatives, inherent in this technology, must be successfully addressed.
The State Key Laboratory of Biotherapy, West China Hospital, and College of Life Science, Sichuan University, Chengdu 610041, P.R. China.
Li
L
Ou
Dan
D
Duan
Jinhua
J
Wu
Edouard
E
Nice
Canhua
C
Huang
D000818:Animals; D019295:Computational Biology / Q000379:methods*; D005821:Genetic Techniques; D057166:High-Throughput Screening Assays / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D018406:Virus Physiological Phenomena
D034741:RNA, Small Interfering