Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B.
RNA polymerase II (RNAP II) C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. Here, we identify by affinity purification and mass spectrometry three previously uncharacterized human CTD-interaction domain (CID)-containing proteins, RPRD1A, RPRD1B and RPRD2, which co-purify with RNAP II and three other RNAP II-associated proteins, RPAP2, GRINL1A and RECQL5, but not with the Mediator complex. RPRD1A and RPRD1B can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. Thus, the RPRD proteins are likely to have multiple important roles in transcription.
Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada.
Zuyao
Z
Ni
Jonathan B
JB
Olsen
Xinghua
X
Guo
Guoqing
G
Zhong
Eric Dongliang
ED
Ruan
Edyta
E
Marcon
Peter
P
Young
Hongbo
H
Guo
Joyce
J
Li
Jason
J
Moffat
Andrew
A
Emili
Jack F
JF
Greenblatt
D020413:3' Untranslated Regions; D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D053484:RecQ Helicases / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; C433812:POLR2M protein, human; C506732:RECQL5 protein, human; C571028:RPAP2 protein, human; C470426:RPRD1A protein, human; C571225:RPRD1B protein, human; D012097:Repressor Proteins; D012319:RNA Polymerase II; D053484:RecQ Helicases