Positive charge of chitosan retards blood coagulation on chitosan films.
In this study, a series of chitosan films with different protonation degrees were prepared by deacidification with NaOH aqueous or ethanol solutions. The films were then used as a model to investigate the effects of the positive charge of chitosan on blood coagulation. The results showed that the positive charge of chitosan acted as a double-edged sword, in that it promoted erythrocyte adhesion, fibrinogen adsorption, and platelet adhesion and activation, but inhibited activation of the contact system. In contrast to prevailing views, we found that the positive charge of chitosan retarded thrombin generation and blood coagulation on these films. At least two reasons were responsible for this phenomenon. First, the positive charge inhibited the contact activation, and second, the positive charge could not significantly promote the activation of non-adherent platelets in the bulk phase during the early stage of coagulation. The present findings improve our understanding of the events leading to blood coagulation on chitosan films, which will be useful for the future development of novel chitosan-based hemostatic devices.
State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Qing
Q
He
Kai
K
Gong
Qiang
Q
Ao
Tuo
T
Ma
Yufang
Y
Yan
Yandao
Y
Gong
Xiufang
X
Zhang
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001777:Blood Coagulation; D002448:Cell Adhesion; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry; D004912:Erythrocytes / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D006487:Hemostasis; D008422:Materials Testing; D015539:Platelet Activation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D013917:Thrombin / Q000096:biosynthesis
D001672:Biocompatible Materials; D005340:Fibrinogen; D048271:Chitosan; D013917:Thrombin