Interaction of an antituberculosis drug with nano-sized cationic micelle: Förster resonance energy transfer from dansyl to rifampicin in the microenvironment.
In this contribution, we report studies on the interaction of an antituberculosis drug rifampicin (RF) in a macromolecular assembly of CTAB with an extrinsic fluorescent probe, dansyl chloride (DC). The absorption spectrum of the drug RF has been employed to study Förster resonance energy transfer (FRET) from DC, bound to the CTAB micelle using picosecond resolved fluorescence spectroscopy. We have applied a kinetic model developed by Tachiya to understand the kinetics of energy transfer and the distribution of acceptor (RF) molecules around the donor (DC) molecules in the micellar surface with increasing quencher concentration. The mean number of RF molecules associated with the micelle increases from 0.24 at 20 μm RF concentration to 1.5 at 190 μm RF concentration and consequently the quenching rate constant (k(q)) due to the acceptor (RF) molecules increases from 0.23 to 0.75 ns(-1) at 20 and 190 μm RF concentration, respectively. However, the mean number of the quencher molecule and the quenching rate constant does not change significantly beyond a certain RF concentration (150 μm), which is consistent with the results obtained from time resolved FRET analysis. Moreover, we have explored the diffusion controlled FRET between DC and RF, using microfluidics setup, which reveals that the reaction pathway follows one-step process.
Department of Chemical, Biological & Macromolecular Sciences, S N Bose National Centre for Basic Sciences, Salt Lake, Kolkata, India.
Tanumoy
T
Mondol
Subrata
S
Batabyal
Samir Kumar
SK
Pal
D000995:Antitubercular Agents / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D003619:Dansyl Compounds / Q000737:chemistry*; D004058:Diffusion; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D008823:Micelles; D044085:Microfluidics; D012293:Rifampin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D013816:Thermodynamics
D000995:Antitubercular Agents; D002593:Cetrimonium Compounds; D003619:Dansyl Compounds; D008823:Micelles; C029038:dansyl chloride; D012293:Rifampin; D000077286:Cetrimonium