IL-10 suppresses bactericidal response of macrophages against Salmonella Typhimurium.
We report, herein, an attempt to determine whether an IL-10-induced immunological state affects the response of macrophages against Salmonella Typhimurium (ST). Pretreatment with mrIL-10 induced the intracellular invasion of ST into macrophages in a dose-dependent manner. It also activated AKT phosphorylation, cyclin D1, Bcl-X(L), and COX-2 upon ST infection, which may correlate with Salmonella's survival within the macrophages. However, I-κB phosphorylation was shown to be inhibited, along with the expression of TNF-α and MIP-2α mRNA. Therefore, IL-10 not only suppresses the bactericidal response of macrophages against ST, but also ultimately causes infected macrophages to function as hosts for ST replication.
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
Kyoung-Sun
KS
Lee
Eui-Suk
ES
Jeong
Seung-Ho
SH
Heo
Jin-Hee
JH
Seo
Dong-Gu
DG
Jeong
Yang-Kyu
YK
Choi
D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D012480:Salmonella Infections / Q000276:immunology* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000276:immunology* / Q000502:physiology
D016753:Interleukin-10