Inhibition of matrine against gastric cancer cell line MNK45 growth and its anti-tumor mechanism.
Anti-tumor activity and mechanism of matrine is evaluated and investigated. MTT assay showed that the matrine was able to inhibit gastric cancer cell line MNK45 in a dose-dependent manner. The concentration required for 50% inhibition (IC50) was found to be 540 μg/ml. This anti-tumor function was achieved through modulation of the NF-κB, XIAP, CIAP, and p-ERK proteins expression in cell line MNK45. By western blot analysis, we found that expression of NF-κB, XIAP, CIAP, and p-ERK proteins in cell line MNK45 would vary with varying concentration of matrine. These protein interactions possibly play a pivotal role in the regulation of apoptosis, for which further detailed analyzes are need. These results overall indicate that matrine can be used as an effective anti-tumor agent in therapy of gastric cancer.
Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, 310022, China.
Cong
C
Luo
Hai Jun
HJ
Zhong
Li Ming
LM
Zhu
Xian Guo
XG
Wu
Jie Er
JE
Ying
Xiao Hong
XH
Wang
Wang Xia
WX
Lü
Qi
Q
Xu
Yong Liang
YL
Zhu
Jian
J
Huang
D000470:Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology* / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D020672:I-kappa B Proteins; D016328:NF-kappa B; D021122:Protein Subunits; D011807:Quinolizines; D051636:X-Linked Inhibitor of Apoptosis Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D000093842:Matrines