Live monitoring of cargo release from peptide-based hybrid nanocapsules induced by enzyme cleavage.
The miniemulsion process is used as a new route for the preparation of enzyme-responsive nanocapsules with payload-release properties. Peptide-based hybrid nanocapsules are prepared via interfacial polyaddition containing a water-soluble dye that is efficiently encapsulated inside. The influence of the synthetic parameters as the functionality of the peptide and the nature of the dispersed phase on the structure of the nanocapsules were investigated. After redispersion in water, the enzymatic cleavage of the peptide sequence and the release of the fluorescent dye are both monitored in real time. This is evidenced because of the quenching FRET system framing the recognition site in the peptide sequence, and the fluorescence recovery of the self-quenched encapsulated dye respectively.
Max-Planck Institute for Polymer Research, Mainz, Germany.
Julien
J
Andrieu
Niklas
N
Kotman
Matthias
M
Maier
Volker
V
Mailänder
Wolfgang S L
WS
Strauss
Clemens K
CK
Weiss
Katharina
K
Landfester
D004655:Emulsions; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D014051:Toluene 2,4-Diisocyanate / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D004655:Emulsions; D005456:Fluorescent Dyes; D053769:Nanocapsules; D010455:Peptides; D011140:Polyurethanes; D012235:Rhodamines; D014867:Water; D014051:Toluene 2,4-Diisocyanate; D014357:Trypsin; C050829:sulforhodamine 101; D008239:Lysine