Effects of different biomaterials: comparing the bladder smooth muscle cells on waterborne polyurethane or poly-lactic-co-glycolic acid membranes.
Tissue engineering materials have often been used to repair bladder damage caused by conditions, such as infection, resection, inflammation, and trauma. However, the concept of generating a functional urinary bladder using autologous cells obtained from a biopsy specimen combined with a biomaterial scaffold remains a challenge. Previously, we presented a new method for synthesizing a biocompatible, mechanically sound, nontoxic, and cross-linked waterborne polyurethane (WBPU) as a potential material for bladder regeneration. Here, we further evaluated the response of bladder smooth muscle cells (BSMCs) seeded on WBPU membranes in comparison with the gold standard biomaterial, poly-lactic-co-glycolic acid. Specifically, we observed the BSMC attachment, proliferation, and α-actin distribution at 1 day, 3 days, and 5 days after membrane seeding. We found that significantly more cells attached and proliferated on the WBPU membranes after 3 days and 5 days of culture, and the cells exhibited greater organization and a wider distribution of α-actin compared with BSMCs cultured on poly-lactic-co-glycolic acid membranes. These preliminary data offer promise for the use of WBPU biomaterials in bladder tissue engineering.
Department of Urology, West China Hospital, Huaxi Clinical College, SiChuan University, ChengDu, SiChuan, China.
Feng
F
Xu
Yan
Y
Wang
Xia
X
Jiang
Hong
H
Tan
Hong
H
Li
Kun-Jie
KJ
Wang
D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D008567:Membranes, Artificial; D008858:Microscopy, Phase-Contrast; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011140:Polyurethanes / Q000494:pharmacology*; D001743:Urinary Bladder / Q000166:cytology*; D014867:Water / Q000737:chemistry*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011140:Polyurethanes; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid