Momilactione B inhibits protein kinase A signaling and reduces tyrosinase-related proteins 1 and 2 expression in melanocytes.
Momilactone B (MB) is a terpenoid phytoalexin present in rice bran that exhibits several biological activities. MB reduced the melanin content in B16 melanocytes melanin content and inhibited tyrosinase activities. Using transcriptome analysis, the genes involved in protein kinase A (PKA) signaling were found to be markedly altered. B16 cells stimulated with MB had decreased concentrations of cAMP protein kinase A activity, and cAMP-response element-binding protein which is a key transcription factor for microphthalmia-associated transcription factor (MITF) expression. Accordingly, the expression of MITF and its target genes, which are essential for melanogenesis, were reduced. MB thus exhibits anti-melanogenic effects by repressing tyrosinase enzyme activity and inhibiting the PKA signaling pathway which, in turn, decreases melanogenic gene expression.
Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of Korea.
Ji Hae
JH
Lee
Boram
B
Cho
Hee-jin
HJ
Jun
Woo-Duck
WD
Seo
Dong-Woo
DW
Kim
Kang-Jin
KJ
Cho
Sung-Joon
SJ
Lee
D000818:Animals; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D004224:Diterpenes / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis*; D007783:Lactones / Q000378:metabolism*; D008543:Melanins / Q000378:metabolism; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D010088:Oxidoreductases / Q000096:biosynthesis*; D047428:Protein Kinase Inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D059467:Transcriptome
D004224:Diterpenes; D007783:Lactones; D008543:Melanins; D008562:Membrane Glycoproteins; D047428:Protein Kinase Inhibitors; C477203:momilactone B; D010088:Oxidoreductases; C489799:TYRP1 protein, human; D017868:Cyclic AMP-Dependent Protein Kinases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase