Characterization of the PEG layer of sterically stabilized liposomes: a SAXS study.
Synchrotron small-angle X-ray scattering analysis of the bilayer structure of a pharmacologically relevant sterically stabilized liposome system is presented. Describing the electron density profile of the bilayer with the superposition of Gaussian functions, the contribution of the poly(ethylene glycol) (PEG) layers to the total electron density was identified. The changes in the thickness of the PEG layer as well as the distribution of the PEG chains among the outer and inner leaflets of the bilayers were followed by changing the molar ratio of the PEG-lipid and the molar weight of the PEG molecule.
Department of Biological Nanochemistry, Institute of Nanochemistry and Catalysis, Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri út 59-67, H-1025 Budapest, Hungary. varga.zoltan@chemres.hu
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Varga
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Wacha
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Vainio
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Gummel
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Bóta
D004583:Electrons; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011789:Quantum Theory; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D008081:Liposomes; D011092:Polyethylene Glycols