Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection.
Infection by Trichinella spiralis takes place in two distinct phases: one is the intestinal phase and the other is the muscle phase. To evaluate alterations in cytokine production during a T. spiralis infection, we periodically assessed the cytokine production of splenocytes in mice after infection (AI). The levels of Th2-related cytokines immediately increased after the initiation of T. spiralis larval intestinal invasion (1 week AI). These early elevations in the Th2 response might be associated with the innate immune responses of intestine epithelial cells against T. spiralis larval invasion. IL-4 and IL-13 levels reached a peak prior to the initiation of nurse cell formation (2 weeks AI). Additionally, all Th17-related cytokines, except for IL-17, increased slightly until 2 weeks AI. However, expression levels for all of the Th2 and Th17-related cytokines began to decrease after the initiation of nurse cell formation and reached basal levels at 4 weeks AI, except for IL-5. At the same time, the CD4(+)CD25(+)Foxp3(+) T (regulatory T, T(reg)) cell population increased significantly in the spleen. Additionally, the number of cells in the peripheral lymph nodes increased. In conclusion, T. spiralis larva intestinal invasion induced the production of Th2 and Th17 cell-related cytokines, and the cytokines decreased with T(reg) cell-related cytokine.
Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
Shin Ae
SA
Kang
Min Kyoung
MK
Cho
Mi-Kyung
MK
Park
Dong-Hee
DH
Kim
Yeon Chul
YC
Hong
Yong Seok
YS
Lee
Hee-Jae
HJ
Cha
Mee Sun
MS
Ock
Hak Sun
HS
Yu
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology*; D018417:Th1 Cells / Q000378:metabolism* / Q000469:parasitology; D058504:Th17 Cells / Q000378:metabolism* / Q000469:parasitology; D017160:Trichinella spiralis / Q000502:physiology*
D016207:Cytokines