HDL-associated enzymes and proteins in hemodialysis patients.
OBJECTIVE
To evaluate HDL-associated proteins and enzymes and their relation with lipoprotein profile and inflammatory markers in chronic renal patients on hemodialysis.


METHODS
We studied 53 patients under hemodialysis and 32 healthy subjects as controls. We compared plasma lipids, Apoprotein-AI and hs-CRP, as a marker of chronic inflammation. We evaluated proteins and enzymes associated to HDL, involved in several points of lipoprotein metabolism: CETP, paraoxonase and LpPLA2 activities. Hepatic lipase was measured in postheparin plasma.


RESULTS
Patients showed higher triglycerides and lower LDL-, HDL- and total-cholesterol than controls (p<0.05). Also, in comparison with controls, Apoprotein-AI, paraoxonase and hepatic lipase were lower, while CETP was higher (p<0.03). LpPLA2 did not show changes between groups.


CONCLUSIONS
Beyond plasma lipid-lipoprotein profile, other factors could contribute to induce a pro-oxidative and pro-inflammatory status. The protective role of HDL does not only depend on its concentration, but also on its functionality.
Laboratory of Lipids and Lipoproteins, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Junín 954, CP1113AAD, Buenos Aires, Argentina.
Leonardo D
LD
Cacciagiú
Ana I
AI
González
Leonardo
L
Gomez Rosso
Tomás
T
Meroño
Guillermo
G
De Marziani
Alicia
A
Elbert
Gabriela
G
Berg
Fernando
F
Brites
Laura
L
Schreier
D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D004798:Enzymes / Q000097:blood*; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D018709:Statistics, Nonparametric
D053480:Cholesterol Ester Transfer Proteins; D004798:Enzymes; D008075:Lipoproteins, HDL; D002097:C-Reactive Protein