Novel nitric oxide producing probiotic wound healing patch: preparation and in vivo analysis in a New Zealand white rabbit model of ischaemic and infected wounds.
The treatment of chronic wounds poses a significant challenge for clinicians and patients alike. Here we report design and preclinical efficacy of a novel nitric oxide gas (gNO)-producing probiotic patch for wound healing. Specifically, a wound healing patch using lactic acid bacteria in an adhesive gas permeable membrane has been designed and investigated for treating ischaemic and infected full-thickness dermal wounds in a New Zealand white rabbit model for ischaemic wound healing. Kaplan-Meier survival curves showed increased wound closure with gNO-producing patch-treated wounds over 21 days of therapy (log-rank P = 0·0225 and Wilcoxon P = 0·0113). Cox proportional hazard regression showed that gNO-producing patch-treated wounds were 2·52 times more likely to close compared with control patches (hazard P = 0·0375, score P = 0·032 and likelihood ratio P = 0·0355), and histological analysis showed improved wound healing in gNO-producing patch-treated animals. This study may provide an effective, safe and less costly alternative for treating chronic wounds.
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Physiology, Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Quebec H3A 2B4, Canada.
Mitchell
M
Jones
Jorge G
JG
Ganopolsky
Alain
A
Labbé
Mirko
M
Gilardino
Christopher
C
Wahl
Christopher
C
Martoni
Satya
S
Prakash
D000279:Administration, Cutaneous; D000818:Animals; D004867:Equipment Design; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000627:therapeutic use; D007511:Ischemia / Q000150:complications / Q000188:drug therapy*; D008297:Male; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000627:therapeutic use; D019936:Probiotics / Q000008:administration & dosage*; D011817:Rabbits; D057968:Transdermal Patch; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy* / Q000473:pathology; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology
D016166:Free Radical Scavengers; D009569:Nitric Oxide