Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses.
Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), is the etiological agent of a virulent and lethal disease in common and koi carp. This study aimed to determine the genetic basis underlying the common carp immune response to the CyHV-3 virus. Two common carp lines (R3 and K) were infected with CyHV-3 by immersion. The R3 line presented a 20% higher survival rate compared to the K line and significantly lower viral loads as measured at day 3 post infection (p.i.). Microarray analysis using a common carp slides containing a number of 10,822 60-mer probes, revealed that 581 genes in line K (330 up-regulated, 251 down-regulated) and 107 genes in line R3 (77 up-regulated, 30 down-regulated), showed at least a 2-fold difference in expression at day 3 p.i. compared to day 0. Genes which showed at least a 4-fold difference in expression in both lines were selected as potential markers of a CyHV-3 infection in common carp. Additionally, 76 genes showed at least 2-fold differentially expression between K and R3 lines at day 3 p.i. Significantly higher expression of several immune-related genes including number of those which are involve in pathogen recognition, complement activation, MHC class I-restricted antigen presentation and development of adaptive mucosal immunity was noted in more resistant R3 line. Further real-time PCR based analysis provided evidence for higher activation of CD8(+) T cells in R3 line. This study uncovered wide array of immune-related genes involved into antiviral response of common carp toward CyHV-3. It is also demonstrated that the outcome of this severe disease in large extent could be controlled by genetic factors of the host.
Polish Academy of Sciences, Institute of Ichthyobiology & Aquaculture in Gołysz, Kalinowa 2, 43-520 Chybie, Poland.
Krzysztof Ł
KŁ
Rakus
Ilgiz
I
Irnazarow
Mikołaj
M
Adamek
Leonor
L
Palmeira
Yuriko
Y
Kawana
Ikuo
I
Hirono
Hidehiro
H
Kondo
Marek
M
Matras
Dieter
D
Steinhagen
Barbara
B
Flasz
Graham
G
Brogden
Alain
A
Vanderplasschen
Takashi
T
Aoki
D056704:Adaptive Immunity / Q000235:genetics*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002347:Carps / Q000235:genetics / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D003167:Complement Activation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006564:Herpesviridae / Q000276:immunology*; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology* / Q000662:veterinary*; D054884:Host-Pathogen Interactions; D020411:Oligonucleotide Array Sequence Analysis; D013601:T-Lymphocytes / Q000378:metabolism; D019562:Viral Load
D015703:Antigens, CD; D016207:Cytokines; D029941:Fish Proteins; D005819:Genetic Markers