Differential effect of isoflurane, medetomidine, and urethane on BOLD responses to acute levo-tetrahydropalmatine in the rat.
Levo-tetrahydropalmatine (l-THP) has shown significant promise in preclinical and clinical studies to treat drug addiction. Pharmacological MRI methods can elucidate the regional cerebral effects of l-THP, but there are potential confounds from the use of general anesthesia. To investigate the possible anesthetic-drug interactions for the pharmacological MRI result of acute l-THP, we examined acute blood oxygen level-dependent responses of both 5 and 20 mg/kg l-THP in naïve rats during general anesthesia achieved with three agents: isoflurane, medetomidine, and urethane. We found that with acute l-THP administration, isoflurane revealed the smallest blood oxygen level-dependent activation areas. In addition, urethane had the most activation areas; however, they were all negative. Medetomidine showed mixed positive and negative activations. Region-specific interactions were found between the l-THP-induced blood oxygen level-dependent responses and the anesthetic agents.
Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Xiping
X
Liu
Rupeng
R
Li
Zheng
Z
Yang
Anthony G
AG
Hudetz
Shi-Jiang
SJ
Li
D018681:Anesthetics, General / Q000008:administration & dosage*; D000818:Animals; D001600:Berberine Alkaloids / Q000008:administration & dosage*; D001783:Blood Flow Velocity / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects* / Q000502:physiology; D004347:Drug Interactions; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D018681:Anesthetics, General; D001600:Berberine Alkaloids; C014215:tetrahydropalmatine