Effect of diet composition and mixture of selected food additives on the erythrocytic system and iron metabolism in peripheral blood of male rats.
BACKGROUND
Metabolic processes of food additives which are "exogenous xenobiotics" are catalysed, primarily, by enzymes located in microsomes of hepatocytes affiliated to P-450 cytochrome superfamily, containing iron. The aim of the study was to investigate the effect of diet composition and selected food additives on the erythrocyte system and iron metabolism in peripheral blood of male rats.


METHODS
The experiment was carried out on 30 male rats sorted into three equinumerous groups. For drinking animals received pure, settled tap water, animals from group III were receiving additionally an aqueous solution of sodium (nitrate), potassium nitrite, benzoic acid, sorbic acid and monosodium glutamate.


RESULTS
Ascertained a significant effect of changes in diet composition on the increase in hematocrit marker value and the count of red blood cells in blood of animals examined. Used food additives diminished hemoglobin concentration, hematocrit value and red blood cell count, diminishing also iron concentration in serum, the total iron binding capacity and transferrin saturation with iron.


CONCLUSIONS
Analysis of the results allowed ascertain adverse changes in values of the erythrocytic system markers, occurring under the influence of the applied mixture of food additives. Used food additives change the iron metabolism, most likely from the necessity of applied xenobiotics biotransformation by heme-containing monoxygenases of P-450 cytochrome.
Department of Human Nutrition Physiology, West Pomeranian University of Technology in Szczecin, Szczecin, Poland. joanna.sadowska@zut.edu.pl
Joanna
J
Sadowska
Magdalena
M
Kuchlewska
D000818:Animals; D004032:Diet; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005503:Food Additives / Q000009:adverse effects* / Q000032:analysis*; D006400:Hematocrit; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D019266:Iron, Dietary / Q000097:blood*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
D005503:Food Additives; D019266:Iron, Dietary