Global analysis of non-coding small RNAs in Arabidopsis in response to jasmonate treatment by deep sequencing technology.
In plants, non-coding small RNAs play a vital role in plant development and stress responses. To explore the possible role of non-coding small RNAs in the regulation of the jasmonate (JA) pathway, we compared the non-coding small RNAs between the JA-deficient aos mutant and the JA-treated wild type Arabidopsis via high-throughput sequencing. Thirty new miRNAs and 27 new miRNA candidates were identified through bioinformatics approach. Forty-nine known miRNAs (belonging to 24 families), 15 new miRNAs and new miRNA candidates (belonging to 11 families) and 3 tasiRNA families were induced by JA, whereas 1 new miRNA, 1 tasiRNA family and 22 known miRNAs (belonging to 9 families) were repressed by JA.
School of Life Sciences, Tsinghua University, Beijing 100084, China.
Bosen
B
Zhang
Daoxin
D
Xie
Zhiping
Z
Jin
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics*; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology*; D018749:RNA, Plant / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
D003517:Cyclopentanes; D035683:MicroRNAs; D054883:Oxylipins; D018749:RNA, Plant; D034741:RNA, Small Interfering; D058727:RNA, Small Untranslated; C011006:jasmonic acid