Investigation of the antiviral properties of copper iodide nanoparticles against feline calicivirus.
This study demonstrated the antiviral properties of copper iodide (CuI) nanoparticles against the non-enveloped virus feline calicivirus (FCV) as a surrogate for human norovirus. The effect of CuI nanoparticles on FCV infectivity to Crandell-Rees feline kidney (CRFK) cells was elucidated. The infectivity of FCV to CRFK cells was greatly reduced by 7 orders of magnitude at 1000μgml(-1) CuI nanoparticles. At the conditions, electron spin resonance (ESR) analysis proved hydroxyl radical production in CuI nanoparticle suspension. Furthermore, amino acid oxidation in the viral capsid protein of FCV was determined by nanoflow liquid chromatography-mass spectrometric (nano LC-MS) analysis. The use of CuI nanoparticles showed extremely high antiviral activity against FCV. The high antiviral property of CuI nanoparticles was attributed to Cu(+), followed by ROS generation and subsequent capsid protein oxidation. CuI nanoparticles could be proposed as useful sources of a continuous supply of Cu(+) ions for efficient virus inactivation. Furthermore, this study brings new insights into toxic actions of copper iodide nanoparticles against viruses.
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Nozomi
N
Shionoiri
Tetsuya
T
Sato
Yoshie
Y
Fujimori
Tsuruo
T
Nakayama
Michiko
M
Nemoto
Tadashi
T
Matsunaga
Tsuyoshi
T
Tanaka
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017927:Calicivirus, Feline / Q000187:drug effects* / Q000472:pathogenicity; D002213:Capsid; D002415:Cats; D002460:Cell Line; D003300:Copper / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D007454:Iodides / Q000494:pharmacology*; D053768:Metal Nanoparticles; D038301:Virus Inactivation / Q000187:drug effects
D000998:Antiviral Agents; D007454:Iodides; D003300:Copper