Effect of polychlorinated biphenyls 118 and 153 on Th1/Th2 cells differentiation.
The objective of this study was evaluate the effect of coplanar polychlorinated biphenyls (PCB) 118-congener (PCB like-dioxin) and noncoplanar PCB 153-congener (PCB no like-dioxin) on differentiation of humans T-CD4+ lymphocytes into Th1 or Th2 subpopulations. Peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteers (aged 25-30 years); T-CD4+ lymphocytes were separated from PBMC. Then, the differentiation of T-CD4+ cells into Th1 or Th2 subpopulation was performed and the intracellular cytokines analyses were assessed. No effect on IFNγ (produced by Th1 cells) production was observed when the cells were treated with both PCBs congeners. However, the PCB 118-congener induced an increase of IL-4-producing T-CD4 cells (produced by Th2 cells), PCB153 not exerted any effect on IL-4 production. The clinical significance of our data is uncertain, therefore, more studies are necessary in order to elucidate the effects generated in exposed human individual.
Departamento de Toxicología Ambiental, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Octavio
O
Gaspar-Ramírez
Francisco Javier
FJ
Pérez-Vázquez
Lucia Guadalupe
LG
Pruneda-Álvarez
Sandra Teresa
ST
Orta-García
Roberto
R
González-Amaro
Iván Nelinho
IN
Pérez-Maldonado
D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D008297:Male; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
C508598:IL4 protein, human; D015847:Interleukin-4; C070055:2,3',4,4',5-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl