Label free detection of CD4+ and CD8+ T cells using the optofluidic ring resonator.
We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
Department of Biological Engineering, 240D Bond Life Sciences Center, 1201 East Rollins Street, University of Missouri, Columbia, MO 65211, USA. jtgdg5@mizzou.edu
John T
JT
Gohring
Xudong
X
Fan
D015374:Biosensing Techniques / Q000295:instrumentation; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology / Q000502:physiology; D006801:Humans; D018655:Lymphocyte Count / Q000295:instrumentation / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008954:Models, Biological; D043942:Nanotubes / Q000737:chemistry; D011379:Prognosis; D013194:Staining and Labeling