Influence of gender and estrous cycle on plasma and renal catecholamine levels in rats.
Several studies have demonstrated that gonadal hormones show significant effects on the brain and signaling pathways of effector organs/cells that respond to neurotransmitters. Since little information is available concerning the impact of male and female gonadal hormones on the renal and peripheral sympathetic system, the objective of this study was to further assess whether and how the renal content and plasma concentration of catecholamines are influenced by gender and the estrous cycle in rats. To achieve this, males Wistar rats were divided into 4 groups: (i) sham (i.e., control), (ii) gonadectomized, (iii) gonadectomized and nandrolone decanoate replacement at physiological levels or (iv)  gonadectomized and nandrolone decanoate replacement at high levels. Female Wistar rats were divided into 6 groups: (i) ovariectomized (OVX), (ii) estrogen replacement at physiological levels and (iii) estrogen replacement at at high levels, (iv) progesterone replacement at physiological levels and (v) progesterone replacement at at high levels, and (vi) sham. The sham group was subdivided into four subgroups: (i) proestrus, (ii) estrus, (iii) metaestrus, and (iv) diestrus. Ten days after surgery, the animals were sacrificed and their plasma and renal catecholamine levels measured for intergroup comparisons. Gonadectomy led to an increase in the plasma catecholamine concentration in females, as well as in the renal catecholamine content of both male and female rats. Gonadectomized males also showed a lower level of plasma catecholamine than the controls. The urinary flow, and the fractional excretion of sodium and chloride were significantly increased in gonadectomized males and in the OVX group when compared with their respective sham groups.
Programa de Pós-Graduação em Ciências Fisiológicas, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Av. Marechal Campos, 1468, 29040-755 Vitória, ES, Brazil.
Helena L
HL
Gomes
Jones B
JB
Graceli
Washington L S
WL
Gonçalves
Roger L
RL
dos Santos
Gláucia R
GR
Abreu
Nazaré S
NS
Bissoli
José G P
JG
Pires
Maria A
MA
Cicilini
Margareth R
MR
Moysés
D000818:Animals; D002395:Catecholamines / Q000097:blood* / Q000378:metabolism*; D002712:Chlorides / Q000652:urine; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D030762:Estrous Cycle / Q000097:blood* / Q000378:metabolism*; D005260:Female; D042341:Gonadal Hormones / Q000008:administration & dosage / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009277:Nandrolone / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D009919:Orchiectomy / Q000379:methods / Q000706:statistics & numerical data; D010052:Ovariectomy / Q000379:methods / Q000706:statistics & numerical data; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D012964:Sodium / Q000652:urine; D014554:Urination / Q000187:drug effects / Q000502:physiology
D002395:Catecholamines; D002712:Chlorides; D042341:Gonadal Hormones; D011374:Progesterone; D004958:Estradiol; D009277:Nandrolone; D012964:Sodium; D000077603:Nandrolone Decanoate