Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)- and heparin (KRSR)-binding peptides.
Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine-glycine-aspartic acid (RGD) and lysine-arginine-serine-arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid-etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly-L-lysine-graft-poly(ethylene glycol) (PLL-g-PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm⁻² KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm⁻² RGD (KRSR/RGD-1); (iii) 1.26 pmol cm⁻² RGD (KRSR/RGD-2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48-780.28 N mm), bone-to-implant contact (BIC) (35.22%-41.49%), and new bone fill (28.58%-30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL-g-PEG polymer does not increase BIC, bone fill, or interfacial shear strength.
Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH-3010 Berne, Switzerland. nina.broggini@zmk.unibe.ch
Nina
N
Broggini
Samuele
S
Tosatti
Stephen J
SJ
Ferguson
Martin
M
Schuler
Marcus
M
Textor
Michael M
MM
Bornstein
Dieter D
DD
Bosshardt
Daniel
D
Buser
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D020007:Implants, Experimental; D016023:Integrins / Q000378:metabolism*; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D016348:Osseointegration / Q000502:physiology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature
D020099:Coated Materials, Biocompatible; D016023:Integrins; D009842:Oligopeptides; C437191:lysyl-arginyl-seryl-arginine; C443714:polylysine-graft-(poly(ethylene glycol)); D011107:Polylysine; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D006493:Heparin