Evaluation of an immunodot blot technique for the detection of antibodies against Taenia solium larval antigens.
Immunodiagnostic tests represent an important tool for diagnosis of cysticercosis, the disease caused by cysticerci of Taenia solium. Accurate diagnosis of neurocysticercosis (NCC) requires costly neuroimaging techniques (magnetic resonance imaging and computed tomography), which are seldom affordable for people in endemic countries. Hence, new low-cost diagnostic methods offering good sensitivity and specificity are needed. Here, we studied four immunodiagnostic tests immunodot blot Tsol-p27, a commercial ELISA, and Western blot Tsol-p27/TsolHSP36, and compared them with a commercial enzyme-linked immunoelectrotransfer blot (EITB) that we regarded as the gold standard method. The analyzed serum samples were obtained from 160 patients: 94 epileptics suspected of NCC, six individuals confirmed NCC-positive, and 60 with positive (30) or negative (30) serology for Chagas diseases. Of the 100 serum samples from epileptic patients, 13 were positive and 87 negative by EITB. Compared to Western blot Tsol-p27, immunodot blot Tsol-p27 offered similar specificity (97.8% vs. 95.6%) but better sensitivity (86.7% vs. 76.4%). The ELISA was similar to the immunodot blot Tsol-p27 regarding both sensitivity and specificity. Western blot TsolHSP36 provided the lowest sensitivity (61.9%) and specificity (86.1%). None of the antibodies in the serum samples from the Chagas control groups were recognized by immunodot blot Tsol-p27. Our results indicate that the immunodot blot Tsol-p27 provides good sensitivity and specificity. Furthermore, considering the simplicity and low cost of this test, it might be preferable as a diagnostic method in poorly equipped laboratories in endemic countries.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, P.O. Box 280, SE-171 77, Stockholm, Sweden.
Fernando
F
Salazar-Anton
Aleyda
A
Tellez
Johan
J
Lindh
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003551:Cysticercosis / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007814:Larva / Q000276:immunology; D010274:Parasitology / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D041201:Taenia solium / Q000276:immunology*
D000909:Antibodies, Helminth; D011933:Reagent Kits, Diagnostic