Biomolecule-coated metal nanoparticles on titanium.
Immobilizations of nanoparticles and biomolecules on biocompatible substrates such as titanium are two promising approaches to bringing new functionalities to Ti-based biomaterials. Herein, we used a variety of X-ray spectroscopic techniques to study and better understand metal-thiolate interactions in biofunctionalized metal nanoparticle systems supported on Ti substrates. Using a facile one-step procedure, a series of Au nanoparticle samples with varied biomolecule coatings ((2-mercatopropionyl)glycine (MPG) and bovine serum albumin (BSA)) and biomolecule concentrations are prepared. Ag and Pd systems are also studied to observe change with varying metal composition. The structure and properties of these biomolecule-coated nanoparticles are investigated with scanning electron microscopy (SEM) and element-specific X-ray techniques, including extended X-ray absorption fine structure (Au L(3)-edge), X-ray absorption near-edge structure (Au L(3), Ag L(3), Pd L(3), and S K-edge), and X-ray photoelectron spectroscopy (Au 4f, Ag 3d, Pd 3d, and S 2p core level). It was found that, by comparison of SEM and X-ray spectroscopy results, the coating of metal nanoparticles with varying model biomolecule systems can have a significant effect on both surface coverage and organization. This work offers a facile chemical method for bio- and nanofunctionalization of Ti substrates as well as provides a physical picture of the structure and bonding of biocoated metal nanoparticles, which may lead to useful applications in orthopedics and biomedicine.
Department of Chemistry and Institute for Research in Materials, Dalhousie University, Halifax, NS B3M 4J3, Canada.
Stephen L
SL
Christensen
Amares
A
Chatt
Peng
P
Zhang
D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D010165:Palladium / Q000737:chemistry; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D012834:Silver; D010165:Palladium; D014025:Titanium; D005998:Glycine