Super-resolution fluorescence imaging of chromosomal DNA.
Super-resolution microscopy is a powerful tool for understanding cellular function. However one of the most important biomolecules - DNA - remains somewhat inaccessible because it cannot be effectively and appropriately labeled. Here, we demonstrate that robust and detailed super-resolution images of DNA can be produced by combining 5-ethynyl-2'-deoxyuridine (EdU) labeling using the 'click chemistry' approach and direct stochastic optical reconstruction microscopy (dSTORM). This method can resolve fine chromatin structure, and - when used in conjunction with pulse labeling - can reveal the paths taken by individual fibers through the nucleus. This technique should provide a useful tool for the study of nuclear structure and function.
Department of Biotechnology and Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg, Germany.
Patrick J M
PJ
Zessin
Kieran
K
Finan
Mike
M
Heilemann
D002467:Cell Nucleus / Q000378:metabolism; D002877:Chromosomes, Human / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D057930:Click Chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D013194:Staining and Labeling
C031086:5-ethynyl-2'-deoxyuridine; D003857:Deoxyuridine