Cloning of metK from Actinoplanes teichomyceticus ATCC31121 and effect of its high expression on antibiotic production.
A metK gene encoding S-adenosyl-L-methionine synthetase was cloned from the non-Streptomyces actinomycetes, Actinoplanes teichomyceticus ATCC31121. In order to evaluate the effect of the metK expression on antibiotic production in actinomycetes, an expression vector harboring the metK gene was constructed and introduced into Streptomyces lividans TK24 and A. teichomyceticus, and the antibiotic production of the exconjugants was assessed. As a result, it was determined that the expression of metK induced 17-fold and 2.2-fold increases in actinorhodin production from S. lividans TK24 and teicoplanin production from A. teichomyceticus, respectively, compared with the control strains.
Department of Food Science and Biotechnology, Kyungnam University, Changwon 631-701, Korea.
Du-Yeong
DY
Kim
Yong-Il
YI
Hwang
Sun-Uk
SU
Choi
D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D008716:Methionine Adenosyltransferase / Q000235:genetics* / Q000378:metabolism; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008716:Methionine Adenosyltransferase