Time-dependent profiles of transcripts encoding lignocellulose-modifying enzymes of the white rot fungus Phanerochaete carnosa grown on multiple wood substrates.
The abundances of nine transcripts predicted to encode lignocellulose-modifying enzymes were measured over the course of Phanerochaete carnosa cultivation on four wood species. Profiles were consistent with sequential decay; transcripts encoding lignin-degrading peroxidases featured a significant substrate-dependent response. The chitin synthase gene was identified as the optimal internal reference gene for transcript quantification.
Department of Chemical Engineering & Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.
Jacqueline
J
Macdonald
Emma R
ER
Master
D004798:Enzymes / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D008031:Lignin / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014934:Wood / Q000378:metabolism / Q000382:microbiology*
D004798:Enzymes; C036909:lignocellulose; D008031:Lignin