Embryo production by parthenogenetic activation and fertilization of in vitro matured oocytes from Cebus apella.
The efficiency of in vitro fertilization (IVF) depends on the viability of spermatozoa. For capuchin monkeys (Cebus apella), in vitro capacitation of spermatozoa is challenging because of their unique seminal coagulum. Motile spermatozoa can be obtained after liquefaction of the semen coagulum in coconut water-based solution. The objective of the present study was to establish an optimal in vitro maturation (IVM) protocol for capuchin monkeys and to observe the effect of follicle stimulating hormone (FSH) and luteinising hormone (LH) on IVF and parthenogenetic activation (PA) of oocytes collected from unstimulated females. We assessed spermatozoa quality after recovery from seminal coagulum using the solution ACP-118® as an extender. Oocytes were matured in vitro for 36 or 40 h and subjected to IVF or PA by applying ionomycin combined either with 6-dimethylaminopurine (6-DMAP) or roscovitine. In total, 87% of oocytes reached metaphase II (MII) after 40 IVM and 4-cell embryo production was obtained after IVF and parthenogenesis using ionomycin/6-DMAP. ACP-118® was used successfully to harvest viable spermatozoa from semen coagulum and in the preservation of spermatozoa, which were able to fertilize oocytes in vitro.
Rua Augusto Corrêa, Campus Básico, CEP 66075-110, Belém, Pará, Brazil. juliannesilvabio@gmail.com
Julianne S
JS
Lima
Danuza L
DL
Leão
Rafael V
RV
Sampaio
Adriel B
AB
Brito
Regiane R
RR
Santos
Moysés S
MS
Miranda
Otávio M
OM
Ohashi
Sheyla F S
SF
Domingues
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002428:Cebus; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008677:Metaphase / Q000187:drug effects*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010312:Parthenogenesis / Q000187:drug effects / Q000502:physiology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D000077546:Roscovitine; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects
D006728:Hormones; D047428:Protein Kinase Inhibitors; D011687:Purines; D000077546:Roscovitine; C001020:N(6),N(6)-dimethyladenine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000225:Adenine