Liquid-phase packaging of a glucose oxidase solution with parylene direct encapsulation and an ultraviolet curing adhesive cover for glucose sensors.
We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals.
The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. s_takamatsu@activemail.jp
Seiichi
S
Takamatsu
Hisanori
H
Takano
Binh-Khiem
BK
Nguyen
Tomoyuki
T
Takahata
Eiji
E
Iwase
Kiyoshi
K
Matsumoto
Isao
I
Shimoyama
D000268:Adhesiveness / Q000528:radiation effects; D000269:Adhesives / Q000494:pharmacology / Q000528:radiation effects; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods; D004349:Drug Packaging / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000032:analysis; D006801:Humans; D008954:Models, Biological; D009994:Osmolar Concentration; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D012996:Solutions / Q000737:chemistry; D014466:Ultraviolet Rays; D014992:Xylenes / Q000737:chemistry / Q000494:pharmacology*
D000269:Adhesives; D011108:Polymers; D012996:Solutions; D014992:Xylenes; C011055:parylene; D005949:Glucose Oxidase; D005947:Glucose