[Process optimization for extraction of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication].
OBJECTIVE
To study the optimal extraction process of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication.


METHODS
An orthogonal experimental design of L9 (3(4)) was used to investigate the effects of ultrasonication time, extraction temperature and extraction time on the extraction ratio, sugar content and immune stimulating activity (mouse splenocyte metabolic activity measured with MTT colorimetry) of the polysaccharides and the optimal extraction process was evaluated.


RESULTS
Ultrasonication treatment had the most remarkable effect on the immune stimulating activity of the polysaccharides. The optimal extraction process for extraction of immune active polysaccharides was as follows: ultrasonication for 30min, extraction temperature at 80 degrees C and water extraction time for 2h.


CONCLUSIONS
Ultrasonication can be used as a useful technique for extraction of immune active polysaccharides from Fomes fomentarius.
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. hegaishan@sina.com.cn
Gai-Yi-Nan
GY
He
Lin-Sheng
LS
Lei
Jie
J
Chen
Chuan-Lin
CL
Yu
Jing
J
Ren
Na-Na
NN
Chen
D000818:Animals; D055453:Coriolaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
D011134:Polysaccharides; D014867:Water