B-cells and IL-4 promote methylcholanthrene-induced carcinogenesis but there is no evidence for a role of T/NKT-cells and their effector molecules (Fas-ligand, TNF-α, perforin).
Mice deficient either in subtypes of immune cells, cytokines or lytic pathways have been subjected to chemical carcinogenesis by methylcholanthrene to evaluate whether these components of the immune system affect tumor development. Inbred mice of the same genotype but from different sources differed in tumor development in magnitude comparable to that previously attributed to differences in immunocompetence. This suggested that genetic drift between separate inbred colonies of mice and/or environmental factors (e.g., transport of the animals) influenced carcinogenesis. Therefore, littermates were used as control in subsequent experiments. Although deficiency of T-cells, NKT-cells, perforin, Fas-ligand, TNF-α-receptor failed to reveal significant differences in tumor development, the presence of B-cells and IL-4 enhanced tumor development under similar experimental conditions.
Institut für Immunologie, Charité, Campus Benjamin-Franklin, Berlin, Germany.
Thomas
T
Kammertoens
Zhihai
Z
Qin
Dana
D
Briesemeister
Albert
A
Bendelac
Thomas
T
Blankenstein
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology; D004777:Environment; D053222:Fas Ligand Protein / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D055611:Natural Killer T-Cells / Q000276:immunology / Q000378:metabolism; D054353:Perforin / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D053222:Fas Ligand Protein; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; D015847:Interleukin-4; D008748:Methylcholanthrene; D007371:Interferon-gamma