Nitrogen sources of Oligoporus placenta and Trametes versicolor evaluated in a 2(3) experimental plan.
Four full-factorial 2(3) experimental plans were applied to evaluate the nitrogen (N) sources of Oligoporus placenta and Trametes versicolor and their interaction with the atmospheric N(2)-assimilating bacterium Beijerinckia acida. The effects of N from peptone, of sapwood and of N from gaseous N(2) on fungal, bacterial and fungal-bacterial activity were investigated. The activities were determined by quantification of biomass, formation of CO(2), consumption of O(2) and laccase activity. The significance of each effect was tested according to t-test recommendation. The activity of both fungi was enhanced by peptone rather than sapwood or gaseous N(2). Nevertheless, comparative studies under an N(2)-free gas mixture as well as under air revealed that the presence of N(2) affected bacterial growth and bacterial-fungal cocultivations. Elemental analysis isotope ratio mass spectrometry (IRMS) of the bacterial and fungal biomass enabled estimation of N transfer and underlined gaseous N(2) as requisite for fungal-bacterial interactions. Combining full-factorial experimental plans with an analytical set-up comprising gas chromatography, IRMS and enzymatic activity allowed synergistic effects to be revealed, fungal N sources to be traced, and symbiotic fungal-bacterial interactions to be investigated.
BAM Federal Institute for Materials Research and Testing, Berlin, Germany. petra.weisshaupt@bam.de
Petra
P
Weisshaupt
Wolfgang
W
Pritzkow
Matthias
M
Noll
D042062:Beijerinckiaceae / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D055453:Coriolaceae / Q000254:growth & development / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism; D056265:Microbial Interactions; D009584:Nitrogen / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D055454:Trametes / Q000254:growth & development / Q000378:metabolism*
D002245:Carbon Dioxide; D042845:Laccase; D009584:Nitrogen; D010100:Oxygen