Anti-diabetic properties of non-polar Toona sinensis Roem extract prepared by supercritical-CO2 fluid.
Toona sinensis Roem (T. sinensis) leaves have been used as a nutritious vegetable and been suggested for medical applications; however, the reported bioactive compounds of T. sinensis leaves are, so far, from high to mid-high polar extracts. Our aims in this study were to reveal the non-polar constituents of the T. sinensis leave extract that were prepared by a method of using a supercritical-CO2 fluid and to investigate the anti-diabetic potential of this extract. Through a GC/MS analysis, we revealed 24 major components of the non-polar T. sinensis leave extract, the most abundant of which was phytol. The non-polar T. sinensis leave extract showed to prevent the progression of diabetes and hepatosteatosis, the rise of triglycerol levels and the decrease of adiponectin levels in the type 2 diabetic mice. Our results suggest that the non-polar extract of T. sinensis leaves prepared using the supercritical-CO2 fluid may contain effective constituents to prevent type 2 diabetes.
Department of Medical Genetics, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Rd., Kaohsiung 80708, Taiwan, ROC.
Tusty-Jiuan
TJ
Hsieh
Yi-Hong
YH
Tsai
Min-Chun
MC
Liao
Ying-Chi
YC
Du
Pei-Jung
PJ
Lien
Chuan-Chia
CC
Sun
Fang-Rong
FR
Chang
Yang-Chang
YC
Wu
D041721:3T3-L1 Cells; D000818:Animals; D002245:Carbon Dioxide / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid; D008401:Gas Chromatography-Mass Spectrometry; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology*
D007004:Hypoglycemic Agents; D010936:Plant Extracts; D002245:Carbon Dioxide