Antibody microarrays for label-free cell-based applications.
The recent advances in microtechnologies have shown the interest of developing microarrays dedicated to cell analysis. In this way, miniaturized cell analyzing platforms use several detection techniques requiring specific solid supports for microarray read-out (colorimetric, fluorescent, electrochemical, acoustic, optical…). Real-time and label-free techniques, such as Surface Plasmon Resonance imaging (SPRi), arouse increasing interest for applications in miniaturized formats. Thus, we focused our study on chemical methods for antibody-based microarray fabrication dedicated to the SPRi analysis of cells or cellular activity. Three different approaches were designed and developed for specific applications. In the first case, a polypyrrole-based chemistry was used to array antibody-microarray for specific capture of whole living cells. In the second case, the polypyrrole-based chemistry was complexified in a three molecular level assembly using DNA and antibody conjugates to allow the specific release of cells after their capture. Finally, in the third case, a thiol-based chemistry was developed for long incubation times of biological samples of high complexity. This last approach was focused on the simultaneous study of both cell type characterization and secretory activity (detection of proteins secreted by cells). This paper describes three original methods allowing a rapid and efficient analysis of cellular sample on-chip using immunoaffinity-based assays.
CREAB Group, SPRAM Laboratory UMR 5819 (CEA-CNRS-UJF Grenoble 1), Institute for Nanosciences and Cryogeny, CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble, France.
Sarah
S
Milgram
Radoslaw
R
Bombera
Thierry
T
Livache
Yoann
Y
Roupioz
D000818:Animals; D006825:Hybridomas / Q000737:chemistry / Q000166:cytology; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry*; D008214:Lymphocytes / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D007074:Immunoglobulin G