Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze].
Catechins are bioprospecting molecules present in tea and any effort towards metabolic engineering of this important moiety would require knowledge on gene regulation. These are synthesized through the activities of phenylpropanoid and flavonoid pathways. Expression regulation of various genes of these pathways namely phenylalanine ammonia-lyase (CsPAL), cinnamate 4-hydroxylase (CsC4H), p-coumarate:CoA ligase (Cs4CL), flavanone 3-hydroxylase (CsF3H), dihydroflavonol 4-reductase (CsDFR) and anthocyanidin reductase (CsANR) was accomplished previously. In depth analyses of the remaining genes namely, chalcone synthase (CsCHS), chalcone isomerase (CsCHI), flavonoid 3'5'-hydroxylase (CsF3'5'H) and anthocyanidin synthase (CsANS) were lacking. The objective of the work was to clone and analyze these genes so as to generate a comprehensive knowledge on the critical genes of catechins biosynthesis pathway. Gene expression analysis was carried out in response to leaf age and external cues (drought stress, abscisic acid, gibberellic acid treatments and wounding). A holistic analysis suggested that CsCHI, CsF3H, CsDFR, CsANS and CsANR were amongst the critical regulatory genes in regulating catechins content.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research, CSIR), P.O. Box No. 6, Palampur-176061, India.
Arti
A
Rani
Kashmir
K
Singh
Paramvir S
PS
Ahuja
Sanjay
S
Kumar
D000040:Abscisic Acid / Q000494:pharmacology; D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D028241:Camellia sinensis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D002392:Catechin / Q000096:biosynthesis*; D003001:Cloning, Molecular; D055864:Droughts; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000494:pharmacology; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D004798:Enzymes; D005419:Flavonoids; D005875:Gibberellins; D000040:Abscisic Acid; D002392:Catechin; C007842:gibberellic acid; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C048797:flavanone 3-dioxygenase; C571205:flavonoid 3'5' dihydroxylase, Camellia sinensis; C118159:anthocyanidin synthase; D000217:Acyltransferases; C020141:flavanone synthetase; D019753:Intramolecular Lyases; C019865:chalcone isomerase