Uncoordinated regulation of atrial natriuretic factor and brain natriuretic peptide in lipopolysaccharide-treated rats.
We investigated the expression and secretion of the natriuretic peptides (NPs) ANF and BNP in lipopolysaccharide (LPS)-induced sepsis and its association with cytokines and other biologically active substances. LPS treatment increased plasma levels of ANF and BNP. The latter increase was larger than the increase in plasma ANF. LPS also increased cardiac content and gene expression of BNP but not of ANF. LPS treatment significantly increased gene expression cytokines, chemokines and proteases, which significantly correlated with BNP gene expression. SB203580, a p38 MAP kinase inhibitor, inhibited the elevation of BNP in plasma. The present work suggests that during inflammation, BNP gene expression and secretion is uniquely related to changes in gene expression in the absence of hemodynamic changes and hence differentiates ANF and BNP as biomarkers of cardiac disease.
Cardiovascular Endocrinology Laboratory, University of Ottawa Heart Institute, Ottawa, Canada.
Tsuneo
T
Ogawa
Adolfo J
AJ
de Bold
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D007093:Imidazoles / Q000008:administration & dosage; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced / Q000276:immunology; D008070:Lipopolysaccharides / Q000633:toxicity; D009206:Myocardium / Q000378:metabolism; D020097:Natriuretic Peptide, Brain / Q000097:blood* / Q000276:immunology; D011725:Pyridines / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000097:blood* / Q000139:chemically induced / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics
D015415:Biomarkers; D016207:Cytokines; D004791:Enzyme Inhibitors; D007093:Imidazoles; D008070:Lipopolysaccharides; D011725:Pyridines; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580