Production of two bacteriocins in various growth conditions produced by gram-positive bacteria isolated from chicken cecum.
Lactobacillus plantarum  CLP29 and  Enterococcus faecium  CLE34 isolated from the cecal contents of young broiler chicks were identified based on physiological and biochemical characteristics, and identification was confirmed by 16S rRNA sequencing. Both bacteria showed a broad range of inhibitory action against bacteria such as  Salmonella  and  Escherichia coli  and produced two peptides, plantaricin CLP29 and enterocin CLE34. Treatment with proteinase K, trypase, or benase resulted in the loss of activity of the two peptides, confirming their proteinaceous nature. The highest activity levels for both bacteria were recorded in de Man - Rogosa - Sharpe agar at pH 5.0, 6.0, and 7.0, at 37 °C. Carbon and nitrogen sources affected the antibacterial activities of the two bacteriocins in different combinations, which suggested that the antibacterial abilities of different bacteriocins produced in nutrient sources were various.
College of Animal Science and Veterinary, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, People's Republic of China.
Qiuju
Q
Wang
Yizhe
Y
Cui
Wenmei
W
Wang
Jili
J
Xu
Li
L
Xu
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004798:Enzymes; D012336:RNA, Ribosomal, 16S