[Study on the change of the content of cantharidin in Mylabris befere and after biortransfermation].
OBJECTIVE
To compare the change of the content of cantharidin in Mylabris before and after biotransformation.


METHODS
The content of cantharidin was determined by gas chromatography (GC). The conditions of GC were as follows: HP-5 column, vanillin as internal standard, the column temperature maintained at 175 degrees for 5 min, rised to 240 degrees C at the speed of 4 degrees C/min and the remained for 5 min.


RESULTS
The GC method was good reproducibility. The content of cantharidin in Myabris before and after botransformation was 0.7% and 1.29% respectively. After biotranformation, the content of cantharidin was increased.


CONCLUSIONS
Mylabris fermentated with Trametes cinnabarina has great significance for increasing the content of cantharidn and provides experimentd basis for efficient use of Mylabris.
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210000, China. xjg12026@163.com
Jin-Guo
JG
Xu
Jian-Hui
JH
Zhang
Jian-Wei
JW
Chen
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D001711:Biotransformation; D002193:Cantharidin / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D001517:Coleoptera / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D008419:Materia Medica / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D012995:Solubility; D055454:Trametes / Q000378:metabolism*
D000970:Antineoplastic Agents; D008419:Materia Medica; D002193:Cantharidin