A case control association study and cognitive function analysis of neuropilin and tolloid-like 1 gene and schizophrenia in the Japanese population.
BACKGROUND
Using a knock-out mouse model, it was shown that NETO1 is a critical component of the NMDAR complex, and that loss of Neto1 leads to impaired hippocampal long term potentiation and hippocampal-dependent learning and memory. Moreover, hemizygosity of NETO1 was shown to be associated with autistic-like behavior in humans.


OBJECTIVE
We examined the association between schizophrenia and the neuropilin and tolloid-like 1 gene (NETO1). First, we selected eight single nucleotide polymorphisms (SNPs) within the NETO1 locus, based on the Japanese schizophrenia genome wide association study (JGWAS) results and previously conducted association studies. These SNPs were genotyped in the replication sample comprised of 963 schizophrenic patients and 919 healthy controls. We also examined the effect of associated SNPs on scores in the Continuous Performance Test and the Wisconsin Card Sorting Test Keio version (schizophrenic patients 107, healthy controls 104).


RESULTS
There were no significant allele-wise and haplotype-wise associations in the replication analysis after Bonferroni correction. However, in meta-analysis (JGWAS and replication dataset) three association signals were observed (rs17795324: p = 0.028, rs8098760: p = 0.017, rs17086492: p = 0.003). These SNPs were followed up but we could not detect the allele-specific effect on cognitive performance measured by the Continuous performance test (CPT) and Wisconsin Card Sorting test (WCST).


CONCLUSIONS
We did not detect evidence for the association of NETO1 with schizophrenia in the Japanese population. Common variants within the NETO1 locus may not increase the genetic risk for schizophrenia in the Japanese population. Additionally, common variants investigated in the current study did not affect cognitive performance, as measured by the CPT and WCST.
Department of Psychiatry, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Aichi-ken, Japan.
Masahiro
M
Banno
Takayoshi
T
Koide
Branko
B
Aleksic
Kazuo
K
Yamada
Tsutomu
T
Kikuchi
Kunihiro
K
Kohmura
Yasunori
Y
Adachi
Naoko
N
Kawano
Itaru
I
Kushima
Masashi
M
Ikeda
Toshiya
T
Inada
Takeo
T
Yoshikawa
Nakao
N
Iwata
Norio
N
Ozaki
D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003071:Cognition / Q000502:physiology*; D005260:Female; D055106:Genome-Wide Association Study; D006801:Humans; D017774:Long-Term Potentiation / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D016194:Receptors, N-Methyl-D-Aspartate; D012559:Schizophrenia / Q000235:genetics* / Q000503:physiopathology*
D008565:Membrane Proteins; C456602:NETO1 protein, human; D016194:Receptors, N-Methyl-D-Aspartate