Coenzyme Q(10) production by immobilized Sphingomonas sp. ZUTE03 via a conversion-extraction coupled process in a three-phase fluidized bed reactor.
A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained even after 15 repetitions (8 h/batch). To fully utilize the residual precursor (para-hydroxybenzoic acid, PHB) in the aqueous phase, the organic phase was replaced with new solution containing 70 mg/L solanesol for each 8 h batch. The CoQ(10) yield of each batch was maintained at a level of about 43 mg/L until the PHB ran out. When solid solanesol was fed to the organic phase for every 8 h batch, CoQ(10) could accumulate and reach a yield of 171.52 mg/L. When solid solanesol and PHB were fed to the conversion system after every 8 h batch, the CoQ(10) yield reached 441.65 mg/L in the organic phase after 20 repetitions, suggesting that the conversion-extract coupled process could enhance CoQ(10) production in the TPFBR.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, PR China.
Lequan
L
Qiu
Hanbing
H
Ding
Weijian
W
Wang
Zhuoyi
Z
Kong
Xuanzhen
X
Li
Yuping
Y
Shi
Weihong
W
Zhong
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism*; D003470:Culture Media; D010226:Parabens / Q000378:metabolism; D020578:Sphingomonas / Q000166:cytology / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
D003470:Culture Media; D010226:Parabens; D013729:Terpenes; D014451:Ubiquinone; C024989:coenzyme Q10; C017719:solanesol; C038193:4-hydroxybenzoic acid