Synthetic poly(ester amine) and poly(amido amine) nanoparticles for efficient DNA and siRNA delivery to human endothelial cells.
Biodegradable poly(ester amine) (PEA)-based and poly(amido amine) (PAA)-based nanoparticles were developed for efficient in vitro siRNA delivery to human umbilical vein endothelial cells (HUVECs). They were screened, characterized, and compared with traditionally studied DNA-containing particles. Several of the polymeric nanoparticles tested were found to be effective for delivering functional siRNA to green fluorescent protein (GFP) + HUVECs, achieving 60%-75% GFP knockdown while maintaining high viability. While PEAs have been used previously to form polyplexes or nanoparticles for DNA delivery, highly effective siRNA delivery in hard-to-transfect human cell types has not been previously reported. PEAs and linear nondendrimeric PAAs were also found to be effective for DNA delivery to HUVECs using GFP-encoding plasmid DNA (up to 50%-60% transfection efficiency). PEAs and PAAs can be separated into groups that form polymeric nanoparticles effective for siRNA delivery, for DNA delivery, or for both.
Department of Biomedical Engineering and the Institute for NanoBioTechnology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA.
Stephany Y
SY
Tzeng
Peter H
PH
Yang
Warren L
WL
Grayson
Jordan J
JJ
Green
D002460:Cell Line; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004058:Diffusion; D042783:Endothelial Cells / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection / Q000379:methods
D053769:Nanocapsules; C531249:Poly(amidoamine); D011073:Polyamines; D011091:Polyesters; D034741:RNA, Small Interfering; C516446:poly(caprolactone-block-ethyleneimine); D004247:DNA