Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter.
Oleaginous yeast Yarrowia lipolytica is an important host for the production of lipid-derived compounds or heterologous proteins. Selection of strong promoters and effective expression systems is critical for heterologous protein secretion. To search for a strong promoter in Y. lipolytica, activities of FBA1, TDH1 and GPM1 promoters were compared to that of TEF1 promoter by constructing GUS reporter fusions. The FBA1 promoter activity was 2.2 and 5.5 times stronger than the TDH1 and GPM1 promoters, respectively. The FBA1IN promoter (FBA1 sequence of -826 to +169) containing an intron (+64 to +165) showed five-fold higher expression than the FBA1 promoter (-831 to -1). The transcriptional enhancement by the 5'-region within the FBA1 gene was confirmed by GPM1::FBA1 chimeric promoter construction. Using the strong FBA1IN promoter, four different S. cerevisiae SUC2 expression cassettes were tested for the SUC+ phenotype in Y. lipolytica. Functional invertase secretion was facilitated by the Xpr2 prepro-region with an additional 13 amino acids of mature Xpr2, or by the native Suc2 signal sequence. However, these two secretory signals in tandem, or the mature Suc2 with no secretory signal, did not direct secretion of functional invertase. Unlike previously reported Y. lipolytica SUC+ strains, our engineered stains secreted most of invertase into the medium.
Central Research and Development, DuPont Co., Wilmington, DE, USA. raymond.hong@usa.dupont.com.
Seung-Pyo
SP
Hong
John
J
Seip
Dana
D
Walters-Pollak
Ross
R
Rupert
Raymond
R
Jackson
Zhixiong
Z
Xue
Quinn
Q
Zhu
D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D025062:Yarrowia / Q000201:enzymology / Q000235:genetics*; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism*
D005656:Fungal Proteins; D013395:Sucrose; D043324:beta-Fructofuranosidase