Cross-reactivity of Fusarium spp. in the Aspergillus Galactomannan enzyme-linked immunosorbent assay.
Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM enzyme-linked immunosorbent assay (EIA). All the isolates produced positive reactions when tested undiluted. These results show cross-reactivity of Fusarium spp. with Aspergillus GM that may constitute a drawback with respect to the specificity of the Platelia EIA.
Dipartimento di Sanita` Pubblica-Microbiologia-Virologia, Universita` degli Studi di Milano, Italy. annamaria.tortorano@unimi.it
Anna Maria
AM
Tortorano
Maria Carmela
MC
Esposto
Anna
A
Prigitano
Anna
A
Grancini
Cristina
C
Ossi
Caterina
C
Cavanna
Giuliana Lo
GL
Cascio
D000328:Adult; D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001230:Aspergillus / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D060585:Fusariosis / Q000175:diagnosis* / Q000382:microbiology; D005670:Fusarium / Q000302:isolation & purification*; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008297:Male; D008351:Mannans / Q000032:analysis*; D008875:Middle Aged; D055815:Young Adult
D008351:Mannans; C012990:galactomannan; D005690:Galactose