Triterpenoid acids and lactones from the leaves of Fadogia tetraquetra var. tetraquetra (Rubiaceae).
Four triterpenoids isolated from the leaves of Fadogia tetraquetra var. tetraquetra, 3beta-hydroxy-11alpha, 12alpha-epoxyoleanan-28,13beta-olide (1), 3beta-hydroxyurs-11-en-28,13beta-olide (2), oleanolic acid (3), and ursolic acid (4), were evaluated for their antiviral and antibacterial properties. Compound 4 showed potent activity against the Semliki Forest virus with an IC50 of 14.7 microM, but was also found to be significantly cytotoxic (68% reduction in cell viability after 24 hours exposure at 50 microM) towards baby hamster kidney (BHK21) host cells. A viability assay on the mammalian human hepatocellular carcinoma (Huh-7) cell line showed no significant effects on intracellular ATP content after 48 hours exposure to compounds 1-4 at this concentration. Compound 4 also inhibited Staphylococcus aureus (MIC 12.5 microM), but was inactive against Enterobacter aerogenes, Escherichia coli, and Pseudomonas aeruginosa. Compounds 1-3 were inactive against all tested bacterial strains at 50 microM concentration.
School of Chemistry, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South Africa. d.mulholland@surrey.ac.uk
Dulcie A
DA
Mulholland
Abdelhafeez M A
AM
Mohammed
Philip H
PH
Coombes
Shafiul
S
Haque
Leena L
LL
Pohjala
Päivi S M
PS
Tammela
Neil R
NR
Crouch
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification*; D002460:Cell Line / Q000187:drug effects; D006224:Cricetinae; D006801:Humans; D007783:Lactones / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*; D000097245:Ursolic Acid
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D007783:Lactones; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid; C569882:3beta-hydroxyurs-11-en-28,13beta-olide