A novel Real Time micro PCR based Point-of-Care device for Salmonella detection in human clinical samples.
Our POC (Point of Care) device is intended to be a diagnostic tool for routine use in the clinical sector. The validation of the whole procedure, including bacterial genomic DNA isolation and the Real Time detection of Salmonella spp., was conducted on 29 clinical stool samples that had been diagnosed with Salmonella spp. by a routine culture technique. The entire process was achieved in a single microfluidic chip within 35 min. In comparison to the culture reference method that is used in the clinical laboratories, this new device performed well in regards to the analytical parameters of sensitivity, specificity and accuracy. Therefore, the POC device reported in this study proved to be very appropriate for the fully integrated analysis system. To the best of our knowledge, this is the first work to report the sample preparation and followed by Real Time PCR (Polymerase Chain Reaction) on a single 2.5 μl chamber chip for the detection of Salmonella spp. bacteria in stool samples.
GAIKER-IK4 Technology Centre, Parque Tecnológico, Edificio 202, 48170, Zamudio, Spain. verdoy@gaiker.es
Dolores
D
Verdoy
Ziortza
Z
Barrenetxea
Javier
J
Berganzo
Maria
M
Agirregabiria
Jesús M
JM
Ruano-López
José M
JM
Marimón
Garbiñe
G
Olabarría
D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D019095:Point-of-Care Systems; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial