tRNA gene identity affects nuclear positioning.
The three-dimensional organization of genomes is dynamic and plays a critical role in the regulation of cellular development and phenotypes. Here we use proximity-based ligation methods (i.e. chromosome conformation capture [3C] and circularized chromosome confrmation capture [4C]) to explore the spatial organization of tRNA genes and their locus-specific interactions with the ribosomal DNA. Directed replacement of one lysine and two leucine tRNA loci shows that tRNA spatial organization depends on both tRNA coding sequence identity and the surrounding chromosomal loci. These observations support a model whereby the three-dimensional, spatial organization of tRNA loci within the nucleus utilizes tRNA gene-specific signals to affect local interactions, though broader organization of chromosomal regions are determined by factors outside the tRNA genes themselves.
Institute of Natural Sciences, Massey University, Auckland, New Zealand.
Chris D M
CD
Rodley
Dave A
DA
Pai
Tyrone A
TA
Mills
David R
DR
Engelke
Justin M
JM
O'Sullivan
D002466:Cell Nucleolus / Q000235:genetics; D002467:Cell Nucleus / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D008239:Lysine / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics
D012343:RNA, Transfer; D008239:Lysine