Innovative collagen nano-hydroxyapatite scaffolds offer a highly efficient non-viral gene delivery platform for stem cell-mediated bone formation.
The ability of nano-hydroxyapatite (nHA) particles developed in-house to act as non-viral delivery vectors is assessed. These nHA particles are combined with collagen to yield bioactive, biodegradable collagen nano-hydroxyapatite (coll-nHA) scaffolds. Their ability to act as gene-activated matrices for BMP2 delivery is demonstrated with successful transfection of mesenchymal stem cells (MSCs) resulting in high calcium production.
Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland.
Caroline M
CM
Curtin
Gráinne M
GM
Cunniffe
Frank G
FG
Lyons
Kazuhisa
K
Bessho
Glenn R
GR
Dickson
Garry P
GP
Duffy
Fergal J
FJ
O'Brien
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D017886:Durapatite / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D050997:Nanomedicine; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D019485:Bone Morphogenetic Proteins; D003094:Collagen; D017886:Durapatite