Measurement of mitochondrial oxygen consumption using a Clark electrode.
Mitochondria require oxygen to produce ATP in sufficient quantities to drive energy-requiring reactions in eukaryotic organisms. The measurement of oxygen consumption rates from isolated mitochondria in vitro is a useful and valuable technique in the research and evaluation of mitochondrial dysfunction and disease since ADP-dependent oxygen consumption directly reflects coupled respiration or oxidative phosphorylation (OXPHOS). This chapter describes the traditional method of mitochondrial polarography using a Clark electrode for measuring coupled respiration in freshly isolated mitochondria from both mammalian tissues and Drosophila melanogaster.
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Zhihong
Z
Li
Brett H
BH
Graham
D000818:Animals; D004331:Drosophila melanogaster / Q000166:cytology; D004566:Electrodes; D008928:Mitochondria / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D011048:Polarography / Q000379:methods*
D010100:Oxygen