Gold nanoparticles as localization markers for direct and live imaging of particle absorption through a Caco-2 cell monolayer using dark-field microscopy.
Recently, efforts have been made to reduce the size of food particles containing functional ingredients, since reducing the size is expected to improve intestinal absorption.  However, the absorption mechanisms have yet to be fully clarified.  Therefore, a microscopy-based method for studying interactions between the particles and intestinal cells is required.  We optimized the experimental conditions for observing gold nanoparticles (AuNPs) on the surface of an unfixed Caco-2 cell using dark-field microscopy (DFM).  Tight junctions were clearly visible with AuNPs on the cells, producing intense scattered light under DFM.  This suggests that AuNPs could be used as localization markers to visualize particle absorption through Caco-2 cells.
National Food Research Institute, National Agriculture and Food Research Organization, Kan-nondai, Tsukuba, Japan. tkobo@affrc.go.jp
Toshiro
T
Kobori
Jun
J
Watanabe
Hidenobu
H
Nakao
D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy; D010316:Particle Size; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D006046:Gold