Biochemical characterization of the exopolysaccharide purified from Laetiporus sulphureus mycelia.
The extracellular polysaccharide (EPS) was isolated from mycelial cultures of Laetiporus sulphureus var. miniatus and purified by DEAE cellulose and Sephadex G-50 column chromatography. The purified EPS (EPS-2-1) was composed of only glucose units and its molecular mass was 6.95 kDa. The chemical structure of EPS-2-1 consisted of a main chain containing (1-->4)-Glcp units with branches at the C-6 position of the chain carrying -Glcp-(1-->4)-linked residues. The effect of purified EPS on immunomodulatory genes and proteins of the Bcl-2 family was observed using cultured U937 human leukemia cells. Of note, the levels of Bax and Bad proteins treated with the EPS (4 mg/ml) were approximately 23- and 18-times higher than those in non-treated cells, respectively. These results may suggest that the EPS purified from the mushroom L. sulphureus is associated with the activation of immunomodulatory mediators, Bax and Bad proteins.
Department of Medical Bioscience, Dong-A University, Busan 604-714, Korea.
Min Jeong
MJ
Seo
Byoung Won
BW
Kang
Jeong Uck
JU
Park
Min Jeong
MJ
Kim
Hye Hyeon
HH
Lee
Yung Hyun
YH
Choi
Yong Kee
YK
Jeong
D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D055453:Coriolaceae / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000235:genetics; D051025:bcl-Associated Death Protein / Q000235:genetics
D011134:Polysaccharides; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein