Inhibitory effects of recombinant porcine interferon-α on high- and low-virulence porcine reproductive and respiratory syndrome viruses.
The inhibitory effects of recombinant porcine interferon alpha (rPoIFN-α) on the propagation of low-virulence PRRSV (lvPRRSV) in MARC-145 cells, and on the progress and severity of high virulence PRRSV (hvPRRSV)-induced infections in pigs, were determined. Pre-treatment of MARC-145 cells with increasing concentrations of rPoIFN-α prior to infection with lvPRRSV decreased the observed cytopathic effects (CPEs) in a concentration-dependent manner. Viral propagation and antibody response were temporarily delayed in swine treated with rPoIFN-α either at the same time as the hvPRRSV challenge was administered or post-challenge. Exposure of challenged animals to rPoIFN-α after the onset of disease symptoms alleviated associated hyperthermia. Variations in lymphocyte subsets indicated that rPoIFN-α treatment might alleviate damage to the immune system or enhance propagation of host cytotoxic T-lymphocytes when the treatment was applied simultaneously with the virus or 1dpc, respectively.
Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Shijuan
S
Dong
Yanbo
Y
Yin
Shiyuan
S
Shen
Yanyan
Y
Guo
Minjie
M
Gao
Wanhua
W
Zhang
Yumin
Y
Zhu
Ruisong
R
Yu
Zhongping
Z
Shi
Zhen
Z
Li
D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D016898:Interferon-alpha / Q000494:pharmacology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors; D014774:Virulence
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016898:Interferon-alpha; D012367:RNA, Viral; D011994:Recombinant Proteins