Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital.
Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among brazilian clinical isolates.
Instituto de Biociencias de Botucatu, Departamento de Microbiologia e Imunologia, Universidade Estadual Paulista, Distrito de Rubiao Jr. s/n, Rubiao Jr., Botucatu, SP, Brazil. chcamargo@fmb.unesp.br
Carlos Henrique
CH
Camargo
Ariane
A
Bruder-Nascimento
Alessandro Lia
AL
Mondelli
Augusto Cezar
AC
Montelli
Terue
T
Sadatsune
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology; D005798:Genes, Bacterial; D005838:Genotype; D006779:Hospitals, Public; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D015378:Imipenem; D002442:Ceftazidime; D001618:beta-Lactamases; D000077731:Meropenem