Neomycin and carbodiimide crosslinking as an alternative to glutaraldehyde for enhanced durability of bioprosthetic heart valves.
Glutaraldehyde cross-linked porcine aortic valves, referred to as bioprosthetic heart valves (BHVs), are often used in heart valve replacements. Glutaraldehyde does not stabilize glycosaminoglycans (GAGs) and they are lost during preparation, in vivo implantation, cyclic fatigue, and storage. We report that binding of neomycin, a hyaluronidase inhibitor, to the tissues with carbodiimide cross-linking improves GAG retention without reducing collagen and elastin stability. It also led to improved biomechanical properties. Neomycin carbodiimide cross-linking did not significantly reduce calcification in a rat subdermal implantation model when they were stored in formaldehyde after cross-linking. Removal of formaldehyde storage significantly reduced calcification.
Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
Joshua
J
Leong
Amy
A
Munnelly
Brianna
B
Liberio
Leonard
L
Cochrane
Naren
N
Vyavahare
D000818:Animals; D001021:Aortic Valve; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D002114:Calcinosis / Q000517:prevention & control; D002234:Carbodiimides; D003094:Collagen / Q000737:chemistry; D003432:Cross-Linking Reagents; D004549:Elastin / Q000737:chemistry; D005976:Glutaral; D006025:Glycosaminoglycans / Q000737:chemistry; D006350:Heart Valve Prosthesis; D008297:Male; D008422:Materials Testing; D009355:Neomycin; D055550:Protein Stability; D051381:Rats; D017207:Rats, Sprague-Dawley; D034421:Sus scrofa; D016707:Tissue Fixation / Q000379:methods
D001672:Biocompatible Materials; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D006025:Glycosaminoglycans; D003094:Collagen; D004549:Elastin; D009355:Neomycin; D005976:Glutaral