A processive carbohydrate polymerase that mediates bifunctional catalysis using a single active site.
Even in the absence of a template, glycosyltransferases can catalyze the synthesis of carbohydrate polymers of specific sequence. The paradigm has been that one enzyme catalyzes the formation of one type of glycosidic linkage, yet certain glycosyltransferases generate polysaccharide sequences composed of two distinct linkage types. In principle, bifunctional glycosyltransferases can possess separate active sites for each catalytic activity or one active site with dual activities. We encountered the fundamental question of one or two distinct active sites in our investigation of the galactosyltransferase GlfT2. GlfT2 catalyzes the formation of mycobacterial galactan, a critical cell-wall polymer composed of galactofuranose residues connected with alternating, regioisomeric linkages. We found that GlfT2 mediates galactan polymerization using only one active site that manifests dual regioselectivity. Structural modeling of the bifunctional glycosyltransferases hyaluronan synthase and cellulose synthase suggests that these enzymes also generate multiple glycosidic linkages using a single active site. These results highlight the versatility of glycosyltransferases for generating polysaccharides of specific sequence. We postulate that a hallmark of processive elongation of a carbohydrate polymer by a bifunctional enzyme is that one active site can give rise to two separate types of glycosidic bonds.
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1544, United States.
John F
JF
May
Matthew R
MR
Levengood
Rebecca A
RA
Splain
Christopher D
CD
Brown
Laura L
LL
Kiessling
D019943:Amino Acid Substitution / Q000235:genetics; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D020134:Catalytic Domain; D005700:Galactosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000076002:Hyaluronan Synthases; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D058105:Polymerization; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity / Q000235:genetics
D005700:Galactosyltransferases; C063054:galactosylxylosylprotein 3-beta-galactosyltransferase; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases