O-sulfate groups of heparin are critical for inhibition of ecotropic murine leukemia virus infection by heparin.
There is increasing evidence that soluble glycosaminoglycans such as heparin can interfere with the infectivity of various viruses, including ecotropic murine leukemia viruses (MLVs). The ecotropic MLV, Friend MLV (F-MLV) and the neuropathogenic variants A8 MLV and PVC-211 MLV, were susceptible to heparin-mediated inhibition of infection of NIH 3T3 cells. To investigate the interaction between the envelope glycoprotein (Env) of MLV and heparin, we prepared vesicular stomatitis virus-based pseudotyped viruses carrying the Env of F-, A8, or PVC-211 MLVs. Surface plasmon resonance analyses indicated that the Env of A8 and PVC-211 MLVs had a higher binding activity to heparin than that of F-MLV. We examined the influence of N- or O-sulfation of heparin on binding activity to Env and on the inhibition of the infectivity of MLV and pseudotyped viruses carrying Env. This analysis indicated that the O-sulfate groups of heparin play a major role in determining Env-dependent inhibitory effects.
Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
Yohei
Y
Seki
Misaho
M
Mizukura
Tomomi
T
Ichimiya
Yasuo
Y
Suda
Shoko
S
Nishihara
Michiaki
M
Masuda
Sayaka
S
Takase-Yoden
D000818:Animals; D015536:Down-Regulation; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000502:physiology*; D051379:Mice; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D012192:Retroviridae Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D012376:Rodent Diseases / Q000378:metabolism* / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
D014759:Viral Envelope Proteins; D006493:Heparin