Appreciation of concentration of lipoproteins and apolipoproteins in serum of male rats under the influence of diet change composition and its supplementation with group B vitamins.
BACKGROUND
This study was aimed at exploring, on animal model, how the diet changes, which results in exceeding 5 times the amount of deficit of group B vitamins after diet change, which up to a certain extent imitates supplementation method in people, influences the concentration of apolipoprotein A-I and apolipoprotein B – the components of lipoprotein HDL-C and LDL-C.


METHODS
The research was conducted on 24 WISTAR male rats, aged ca 5 months. The animals were divided into 3 feeding groups: I was fed with basic compound which contained among others full wheat grain and corn grain, group II and III with modified compound in which part of the full wheat grain, from basic compound, was substituted for wheat flour, and 50% of corn with saccharose. Group I and II animals were drinking pure tap water which was left to stand for some time beforehand, group III animals vitamins dissolved in water: B(1), B(2), B(6), and PP – five times exceeded the difference between the amount of basic and modified feed, which in a certain way imitated the supplementation in human food. After one week of animals conditioning, the experiment was conducted for 6 weeks. In their blood serum were determined concentration of glucose (GL), triacyloglycerols (TG), total cholesterol (TC), fraction of HDL-cholesterol (HDLC), fraction of LDL-cholesterol (LDL-C) and apolipoproteins A-I (apo A-I) and apolipoproteins B (apoB). In the dissected muscles and in the animals' livers the amount of fat was determined with Soxhlet's method. The obtained results were analysed with one factor variance by use of statistic computer program Statistica® with application of Duncan test.


RESULTS
Analysing the influence of diet change and its supplementation with chosen group B vitamins on the amount of consumed feed, it was ascertained that in spite of the same calorific value of the used feed, significantly less, compared to other groups, was consumed by the animals from the supplemented group. However, there was no significant gain of body mass in male rats and higher accumulation of pericardial and periintestinal fat tissue. Significantly lower amount of fat, in comparison to animals fed with basic feed, was observed in muscles of animals fed with modified and supplemented feed, however significantly higher amount of fat was found in liver. In animals fed with modified feed compared with animals fed with basic feed higher GL, TG, TC its LDL-C fraction and apoB and decrease of fraction HDL-C concentration was noticed. The applied supplementation with group B vitamins statistically significantly lowered concentration of GL, TG, LDL-C fraction, apoB and increased concentration of TC. Observed decrease concentration of HDL-C fraction, apoA-I, apoB and increased TC in serum of supplemented animals it was statistically insignificant.


CONCLUSIONS
Analysis of the obtained results allowed stating that supplementation of diet with chosen group B vitamins in which full grains were exchanged for wheat flour and saccharose, was favourable to returning to original state of disadvantageous effects brought by change of diet content. It was demonstrated by decrease of glucose, triacyloglycerols, lipoprotein LDL-C and apoB concentration while lipoproteins HDL-C and apoA-I were decreased and increase of total cholesterol in blood of rats under research, although not all changes were statistically significant.
West Pomeranian University of Technology in Szczecin, Poland. Zuzanna.Goluch-Koniuszy@zut.edu.pl
Zuzanna
Z
Goluch-Koniuszy
Aldona
A
Wierzbicka
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D004032:Diet; D019587:Dietary Supplements; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D014803:Vitamin B Complex / Q000008:administration & dosage*
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014803:Vitamin B Complex