Blotting protein complexes from native gels to electron microscopy grids.
We report a simple and generic method for the direct transfer of protein complexes separated by native gel electrophoresis to electron microscopy grids. After transfer, sufficient material remains in the gel for identification and characterization by mass spectrometry. The method should facilitate higher-throughput single-particle analysis by substantially reducing the time needed for protein purification, as demonstrated for three complexes from Thermoplasma acidophilum.
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Martinsried, Germany.
Roland Wilhelm
RW
Knispel
Christine
C
Kofler
Marius
M
Boicu
Wolfgang
W
Baumeister
Stephan
S
Nickell
D013058:Mass Spectrometry; D008854:Microscopy, Electron / Q000379:methods*