Paper-based three-dimensional electrochemical immunodevice based on multi-walled carbon nanotubes functionalized paper for sensitive point-of-care testing.
In this study, electrochemical immunoassay was introduced into the recently proposed microfluidic paper-based analytical device (μPADs). To improve the performance of electrochemical immunoassay on μPAD for point-of-care testing (POCT), a novel wax-patterned microfluidic paper-based three-dimensional electrochemical device (3D-μPED) was demonstrated based on the multi-walled carbon nanotubes (MWCNTs) modified μPAD. Using typical HRP-O-Phenylenediamine-H(2)O(2) electrochemical system, a sandwich immunoassay on this 3D-μPED for sensitive diagnosis of two tumor markers simultaneously in real clinical serum samples was developed with a linear range of 0.001-75.0 UmL(-1) for cancer antigen 125 and 0.05-50.0 ngmL(-1) for carcinoembryonic antigen. In addition, this 3D-μPED can be easily integrated and combined with the recently emerging paper electronics to further develop simple, sensitive, low-cost, disposable and portable μPAD for POCT, public health and environmental monitoring in remote regions, developing or developed countries.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Panpan
P
Wang
Lei
L
Ge
Mei
M
Yan
Xianrang
X
Song
Shenguang
S
Ge
Jinghua
J
Yu
D018394:CA-125 Antigen / Q000097:blood*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D008565:Membrane Proteins / Q000097:blood*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood*; D010209:Paper; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
D018394:CA-125 Antigen; D002272:Carcinoembryonic Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D037742:Nanotubes, Carbon