The cytotoxic effect of combined hyperthermia and taxane chemotherapy on ovarian cancer cells: results of an in vitro study.
OBJECTIVE
Hyperthermic intraperitoneal chemotherapy (HIPEC) is under continuous evaluation as a potential treatment for ovarian cancer. The purpose of this study was to evaluate the effect of chemotherapy, drug concentration and temperature.


METHODS
We examined the combined effects of hyperthermia and taxane chemotherapy on the clonogenic survival of the human ovarian carcinoma SHIN-3 cell line in vitro.


RESULTS
When hyperthermia was combined with chemotherapy, the median lethal dose (LD50) was equivalent regardless of the duration of exposure, and was independent of the exposure temperature. Taxanes showed a similar LD50 over the temperature range tested.


CONCLUSIONS
In our study, hyperthermia does not increase the cytotoxic effects of taxanes, at least for the concentrations and durations tested.
Unit of Gynecological and Mammary Oncologic Surgery, Centre Hospitalier Universitaire Augustin Morvan, Brest, France. matthieu.muller@chu-brest.fr
M
M
Muller
M
M
Chérel
P-F
PF
Dupré
S
S
Gouard
M
M
Collet
J-M
JM
Classe
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D003080:Cold Temperature; D003131:Combined Modality Therapy; D018284:Cystadenocarcinoma, Serous / Q000473:pathology*; D000077143:Docetaxel; D005260:Female; D006801:Humans; D007928:Lethal Dose 50; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology*; D043823:Taxoids / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel