The planar cell polarity pathway and parietal endoderm cell migration.
Parietal endoderm (PE) migration is the first long-range migratory event in the mammalian embryo contributing to the parietal yolk sac. PE migration can be studied in vitro using the F9 teratocarcinoma stem cell model system. We have found that PE migration is directed and modulated via the Planar Cell Polarity (PCP) pathway through Rho/ROCK signaling. Wnt inhibition using sFRP results in a loss of orientation, visualized by Golgi apparatus localization, along with disorganized microtubules and a lack of robust focal adhesions. Small GTPases are downstream of PCP signaling and Rho/ROCK inhibition results in a loss of orientation, whereas inhibition of Rac does not affect PCP. Activation of canonical Wnt signaling combined with Wnt inhibition does not prevent loss of oriented migration. These data support a role for non-canonical Wnt/PCP signaling directing oriented migration of PE.
Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
Kristi
K
Lamonica
Laura
L
Grabel
D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects; D016764:Cell Polarity / Q000187:drug effects; D058732:Embryoid Bodies / Q000378:metabolism; D004707:Endoderm / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051157:Frizzled Receptors / Q000737:chemistry / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors; D000071679:Glycogen Synthase Kinase 3 beta; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology; D010091:Oximes / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D012995:Solubility; D014162:Transfection; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism
C483321:6-bromoindirubin-3'-oxime; D051157:Frizzled Receptors; D007211:Indoles; D010091:Oximes; D047428:Protein Kinase Inhibitors; D000071679:Glycogen Synthase Kinase 3 beta; D054460:rho-Associated Kinases; D038362:Glycogen Synthase Kinase 3; D020744:rac GTP-Binding Proteins