Chemokine receptor 2-V64I and chemokine receptor 5-Delta32 polymorphisms and clinical risk factors of delayed graft function and acute rejection in kidney transplantation.
BACKGROUND
Chemokines and chemokine receptors have a pivotal role in immunity and inflammation. We aimed to evaluate their role in kidney transplant rejection.


METHODS
The association of chemokine (C-C motif) receptor 2 (CCR2)-V64I and CCR5-Delta32 gene polymorphisms with acute rejection (AR) and delayed graft function (DGF) were examined in 100 donor-recipient pairs. The CCR2-V64I and CCR5-Delta32 alleles were determined using polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism, respectively.


RESULTS
No associations were found between donors or recipients' CCR2-V64I and CCR5-Delta32 gene polymorphisms and AR or DGF. Of the characteristics of the donors, recipients, and transplantation, glomerulonephritis as a cause of kidney failure in the recipients was weakly associated with AR (relative risk, 6.1; 95% confidence interval, 0.8 to 46.0; P = .07). Transplantation of kidney from females to males was weakly associated with DGF (relative risk, 5.5; 95% confidence interval, 0.9 to 33.0; P = .06). There was a significant association between AR, but not DGF, and graft loss in the patients (relative risk, 28.6; 95% confidence interval, 1.7 to 487.0; P = .03).


CONCLUSIONS
Our study failed to suggest CCR2-V64I or CCR5-Delta32 gene polymorphisms as risk factors for AR and DGF in kidney transplantation. Sex-matching between donors and recipients should be considered for living donor kidney transplantation.
Physiology Research Center and Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Jalal
J
Azmandian
Ali
A
Mandegary
Azadeh
A
Saber
Maryam
M
Torshabi
Abbas
A
Etminan
Mohammad-Reza
MR
Ebadzadeh
Faramarz
F
Fazeli
Samaneh
S
Soleymani
Atefeh
A
Taghipour
Mohammad-Ali
MA
Karimi
D000208:Acute Disease; D000328:Adult; D051799:Delayed Graft Function / Q000235:genetics*; D005260:Female; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D016030:Kidney Transplantation; D016015:Logistic Models; D008297:Male; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2 / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*; D012307:Risk Factors; D012737:Sex Factors; D014184:Transplantation, Homologous
C515334:CCR2 protein, human; D054390:Receptors, CCR2; D019713:Receptors, CCR5