A sensitive capillary GC-MS method for analysis of topiramate from plasma obtained from single-dose studies.
Topiramate (Topamax®) is an antiepileptic medication used as adjunctive and monotherapy in patients with epilepsy and for migraine prophylaxis. A GC-MS assay was developed that was capable of detecting topiramate plasma concentrations following a single rectal or oral dose administration. Topiramate plasma samples were prepared by solid-phase extraction and were quantified by GC-MS analysis. The topiramate standard curves were split from 0.1 to 4 µg/mL and from 4 to 40 µg/mL in order to give a more accurate determination of the topiramate concentration. The accuracy of the standards ranged from 94.6 to 107.3% and the precision (%CV) ranged from 1.0 to 5.3% for both curves at all concentrations. The %CV for quality controls was <7.6%. The assay is both accurate and precise and will be used to complete future pharmacokinetic studies.
Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA.
Jeannine M
JM
Conway
Angela K
AK
Birnbaum
Susan E
SE
Marino
James C
JC
Cloyd
Rory P
RP
Remmel
D003986:Dibenzocycloheptenes / Q000097:blood; D005632:Fructose / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D000077236:Topiramate
D003986:Dibenzocycloheptenes; D000077236:Topiramate; D005632:Fructose; C002556:cyheptamide