Impact of estradiol, ER subtype specific agonists and genistein on energy homeostasis in a rat model of nutrition induced obesity.
Estrogens are known to be involved in the control of energy homeostasis. Here we investigated the role of ER alpha and ER beta in a model of nutrition induced obesity. Ovariectomized Wistar rats were fed a high fat diet and received either vehicle, E2, ER subtype selective agonists (Alpha and Beta) or genistein. After 10 weeks, body weight, visceral fat, serum leptin, blood lipids, and in the soleus muscle anabolic markers were determined. Treatment with E2 and Alpha decreased body weight, total cholesterol and VLDL. Visceral fat mass, adipocyte size, and serum leptin were reduced by E2, Alpha and Beta. In the soleus muscle, treatment with E2 and Beta modulated Igf1 and Pax7 gene expression and resulted in larger muscle fibers. Our data indicate that blood lipids are affected via ER alpha, whereas activation of ER beta results in an increase of soleus muscle mass. Adipose tissue homeostasis seems to be affected via both ERs.
German Sports University Cologne, Institute of Cardiovascular Research and Sports Medicine, Department of Cellular and Molecular Sports Medicine, Am Sportpark Müngersdorf 6, 50933 Köln, Germany. c.weigt@dshs-koeln.de
Carmen
C
Weigt
Torsten
T
Hertrampf
Nora
N
Zoth
Karl Heinrich
KH
Fritzemeier
Patrick
P
Diel
D017667:Adipocytes / Q000502:physiology; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D059305:Diet, High-Fat; D004734:Energy Metabolism; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000819:agonists / Q000378:metabolism*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D006706:Homeostasis; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D050152:Intra-Abdominal Fat; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D020738:Leptin; D008055:Lipids; C530821:PAX7 protein, rat; D051761:Paired Box Transcription Factors; C548904:insulin-like growth factor-1, rat; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D019833:Genistein