Nanoporous impedemetric biosensor for detection of trace atrazine from water samples.
Trace contamination of ground water sources has been a problem ever since the introduction of high-soil-mobility pesticides, one such example is atrazine. In this paper we present a novel nanoporous portable bio-sensing device that can identify trace contamination of atrazine through a label-free assay. We have designed a pesticide sensor comprising of a nanoporous alumina membrane integrated with printed circuit board platform. Nanoporous alumina in the biosensor device generates a high density array of nanoscale confined spaces. By leveraging the size based immobilization of atrazine small molecules we have designed electrochemical impedance spectroscopy based biosensor to detect trace amounts of atrazine. We have calibrated the sensor using phosphate buffered saline and demonstrated trace detection from river and bottled drinking water samples. The limit of detection in all the three cases was in the femtogram/mL (fg/mL) (parts-per-trillion) regime with a dynamic range of detection spanning from 10 fg/mL to 1 ng/mL (0.01 ppt to 1 ppm). The selectivity of the device was tested using a competing pesticide; malathion and selectivity in detection was observed in the fg/mL regime in all the three cases.
Bioengineering Program, College of Engineering, 1745 N. Fairmount St., Wichita State University, Wichita, KS 67260, USA. pxpichetsurnthorn1@wichita.edu
Pie
P
Pichetsurnthorn
Krishna
K
Vattipalli
Shalini
S
Prasad
D000537:Aluminum Oxide / Q000737:chemistry*; D001280:Atrazine / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D058266:Dielectric Spectroscopy / Q000295:instrumentation; D060766:Drinking Water / Q000032:analysis*; D004867:Equipment Design; D006540:Herbicides / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D045483:Rivers / Q000737:chemistry*; D012680:Sensitivity and Specificity
D060766:Drinking Water; D006540:Herbicides; D000537:Aluminum Oxide; D001280:Atrazine