High-pressure tolerance in Halobacterium salinarum NRC-1 and other non-piezophilic prokaryotes.
In this study, we examined the high-pressure survival of a range of prokaryotes not found in high-pressure environments to determine the effects of adaptations to osmotic and oxidative stresses on piezo-resistance. The pressure survivals of Halobacterium salinarum NRC-1, Deinococcus radiodurans R1, and Chromohalobacter salexigens were compared to that of Escherichia coli MG1655. C. salexigens, which uses the compatible solute ectoine as an osmolyte, was as piezo-sensitive as E. coli MG1655, suggesting that ectoine is not a piezolyte. D. radiodurans R1 and H. salinarum NRC-1, both resistant to oxidative stress, were found to be highly piezo-resistant. H. salinarum NRC-1 showed nearly full survival after pressurization up to 400 MPa; a survival 3.5 log units higher than E. coli MG1655. This piezo-resistance was maintained in H. salinarum NRC-1 for pressurizations up to 1 h. We hypothesize that the high-pressure resistance of H. salinarum NRC-1 is due to a combination of factors including cell envelope structure and the presence of intracellular salts.
Geophysical Laboratory, Carnegie Institution of Washington, 5251 Broad Branch Rd., NW, Washington, DC, 20015, USA. adrienne.kish@igmors.u-psud.fr
Adrienne
A
Kish
Patrick L
PL
Griffin
Karyn L
KL
Rogers
Marilyn L
ML
Fogel
Russell J
RJ
Hemley
Andrew
A
Steele
D019843:Archaeal Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D055353:Chromohalobacter / Q000378:metabolism; D003080:Cold Temperature; D034301:Deinococcus / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D019617:Halobacterium salinarum / Q000378:metabolism*; D009995:Osmosis; D018384:Oxidative Stress; D011312:Pressure; D011387:Prokaryotic Cells / Q000378:metabolism; D012492:Salts / Q000737:chemistry
D019843:Archaeal Proteins; D012492:Salts