Microbial treatment of the monosodium glutamate wastewater by Lipomyces starkeyi to produce microbial lipid.
The monosodium glutamate (MSG) wastewater as a medium was treated by Lipomyces starkeyi to produce microbial lipid in the study. The effect of related factors (initial glucose concentration, inoculation concentration, initial culture pH, and cultivation time) on biomass, lipid production and lipid content was discussed, respectively. According to the experiments, the optimal fermentation conditions were determined: addition of 80g/L glucose, 10% inoculation concentration, initial pH about 5.0, incubation time 96h. Under this condition, the biomass production reached up to 4.61g/L, lipid production and lipid content was 1.14g/L and 24.73%, respectively. Simultaneously, protein and COD removal rate was 78.60% and 74.96%, respectively. The main composition of fatty acid in the resultant lipid was analyzed by gas chromatography-mass spectrometry, which showed: oleic acid (C18:1) 35.85%, palmitic acid (C16:0) 19.91%, palmitoleic acid (C16:1) 17.65%, and myristic acid (C14:0) 16.03%.
Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, No. 27 Shanda South Road, Jinan 250100, PR China.
Jun-Xian
JX
Liu
Qin-Yan
QY
Yue
Bao-Yu
BY
Gao
Zuo-Hao
ZH
Ma
Pei-Dong
PD
Zhang
D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D055160:Lipomyces / Q000187:drug effects / Q000378:metabolism*; D012970:Sodium Glutamate / Q000737:chemistry*; D013997:Time Factors; D014865:Waste Disposal, Fluid; D018508:Water Purification / Q000379:methods*
D003470:Culture Media; D008055:Lipids; D005947:Glucose; D012970:Sodium Glutamate