[Kidney-tonifying and abortion preventing effects of Shou Tai Wan by different extration methods on rats].
OBJECTIVE
To explore the Kidney-tonifying and abortion preventing effect of Shou Tai Wan (STW) by different extration methods on the SD Rats' abortion model.


METHODS
Applied hydroxycarbamide and mifepristone (RU-486) to establish the abortion model of corpus luteum inhibition due to Kidney deficiency (disease-syndorme combination model) on SD, rats. Treated the model rats with STW formula. Observed the uterus condition and recorded the embryo number and the miscarriage rate of each rat. 4 kinds of extractions including water extract of STW (A liquid), alcohol extract of STW (B liquid), after the ethanol water extract residue of STW (C liquid) and B + C liquid. Visual observed the uterine lesions embryos and calculated obortion rate. Used chemluninescence methed to cheek the serum estradiol (E2) and progesterone (P) level. Used quantitative RT-PCR (qRT-PCR) to analyze the different of the PR mRNR between the model group and the treated group.


RESULTS
Compared with the model group, the abortion rate of B + C liquid was greatly deduced and the embryo number of B + C liquid group, the E2 and P levels were obviously increased in the treated groups.


CONCLUSIONS
STW (B + C) has the best effect of tonifying the kidney and preventing abortion.
The First Affiliated Hospital of Guangzhou TCM, Guangzhou 510405, China. gjfkts@hotmail.com
Jie
J
Gao
Song-Ping
SP
Luo
D000022:Abortion, Spontaneous / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D005260:Female; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D012076:Renal Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D012076:Renal Agents; D011374:Progesterone; D004958:Estradiol