Methods in cell biology: analysis of cell polarity in C. elegans embryos.
Cell polarity is a fundamental principle guiding development. Early C. elegans embryos contain a diversity of polarized cell types, ranging from asymmetrically dividing stem cells to a polarized epithelium. Over the past two decades, work using C. elegans embryos has led to mechanistic understandings of many aspects of cell polarity establishment, maintenance and propagation. Here we provide basic methods for researchers interested in using C. elegans embryos to study cell polarity, emphasizing the amenability of C. elegans to quantitative molecular analysis.
MOCA, RWTH Aachen, 52074 Aachen Germany.
Olaf
O
Bossinger
Carrie R
CR
Cowan
D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics / Q000648:ultrastructure*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity / Q000235:genetics; D004625:Embryo, Nonmammalian; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D015388:Organelles / Q000648:ultrastructure; D034622:RNA Interference; D059010:Single-Cell Analysis; D013234:Stem Cells / Q000378:metabolism / Q000648:ultrastructure*
D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D005456:Fluorescent Dyes