Development of iodoacetic acid-based cysteine mass tags: detection enhancement for cysteine-containing peptide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
We developed and characterized 6 new cysteine mass tags for high-sensitivity peptide analysis. The structural features are: (1) iodoacetyl group for thiol tagging, (2) hydrophilic character for reducing sample loss, (3) tertiary amino, quaternary ammonium, or guanidino group for high proton affinity, and (4) no amide bonding for minimizing fragmentation of tag moiety in collision-induced dissociation. By using these tags, 2- to 200-fold MS sensitivity was achieved, compared to control peptide with carbamydomethylation.
SHIMADZU Corporation, Koichi Tanaka Laboratory of Advanced Science and Technology, 1 Kuwabara-cho, Nishinokyo, Nakagyo-ku, Kyoto 604-8511, Japan.
Takashi
T
Shimada
Hiroki
H
Kuyama
Taka-Aki
TA
Sato
Koichi
K
Tanaka
D003545:Cysteine / Q000032:analysis*; D019807:Iodoacetic Acid / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010455:Peptides; D003545:Cysteine; D019807:Iodoacetic Acid