Complete nucleotide sequence of plasmid pST-III from Lactobacillus plantarum ST-III.
The complete nucleotide sequence of the 53,560-bp plasmid pST-III from Lactobacillus plantarum ST-III has been determined. The plasmid contains 42 predicted protein-coding sequences, and the functions of 34 coding sequences could be assigned. Homology analysis for the replication protein and the typical features of the origin of replication suggested that pST-III replicates via the theta-type mechanism. Among the predicted genes, we identified a kdp gene cluster (a high-affinity K(+)-transport system) for the first time in the Lactobacillus genus and a system for osmolyte transport. Analysis of the plasmid-encoded functions and the plasmid-cured experiment showed that the genes of pST-III could serve for the niche adaptations of L. plantarum ST-III and make significant contributions to its viability under hyperosmotic conditions. Furthermore, the relative copy number of pST-III was determined to be 6.79±1.55 copies per cell.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Chen
C
Chen
Lianzhong
L
Ai
Fangfang
F
Zhou
Jing
J
Ren
Kejie
K
Sun
Hao
H
Zhang
Wei
W
Chen
Benheng
B
Guo
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004260:DNA Repair; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics*; D012093:Replicon; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C048817:RepB protein, Bacteria