Cardioactive C₁₉-diterpenoid alkaloids from the lateral roots of Aconitum carmichaeli "Fu Zi".
Bioassay-guided fractionation of an n-BuOH extract of the lateral roots of Aconitum carmichaeli. led to the isolation of 5 cardioactive C(19)-diterpenoid alkaloids: N-deethylaconine (1), beiwutinine (2), hypaconine (3), mesaconine (4), and 15α-hydroxyneoline (5). N-Deethylaconine and beiwutinine are new aconitine-type C(19)-diterpenoid alkaloids. Hypaconine was isolated from this species for the first time. Among them, mesaconine, hypaconine, and beiwutinine showed the strongest cardiac actions on the isolated perfused bullfrog heart. Furthermore, mesaconine has protective effects, including improved inotropic effect and left ventricular diastolic function, on myocardial ischemia-reperfusion injury in rat at a dose of 10(-9) mol/L. However, mesaconine has almost no effect on heart rate.
Department of Medicinal Analysis, West China College of Pharmacy, Sichuan University, Chengdu 610041, P R China.
Xiu-Xiu
XX
Liu
Xi-Xian
XX
Jian
Xiu-Feng
XF
Cai
Ruo-Bing
RB
Chao
Qiao-Hong
QH
Chen
Dong-Lin
DL
Chen
Xiao-Liang
XL
Wang
Feng-Peng
FP
Wang
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D002316:Cardiotonic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D004224:Diterpenes / Q000737:chemistry*; D006321:Heart / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy
D000470:Alkaloids; D002316:Cardiotonic Agents; D004224:Diterpenes