Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin.
Heparin covalently attached to a water-insoluble resin suspended in HIV-infected aqueous buffer or whole blood captures the virus; subsequent physical separation of the immobilized heparin reduced the viral titers by over 80 and 50%, respectively. The detoxification concept has been validated by both circulating an HIV-1 solution through a column packed with the heparin-sepharose beads and successively mixing an HIV-1 solution with fresh beads.
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. rnassar@mit.edu
Roger A
RA
Nassar
Edward P
EP
Browne
Jianzhu
J
Chen
Alexander M
AM
Klibanov
D001769:Blood / Q000821:virology*; D003666:Decontamination / Q000379:methods*; D015497:HIV-1 / Q000302:isolation & purification*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D019562:Viral Load
D006493:Heparin