High-resolution imaging of cellular processes in Caenorhabditis elegans.
Differential interference contrast (DIC) imaging of Caenorhabditis elegans embryogenesis led to a Nobel Prize in Physiology or Medicine (Sulston et al., 1983) as did the first use of green fluorescent protein (GFP) in a transgenic C. elegans (Chalfie et al., 1994). Given that C. elegans is free living, does not require exceptional environmental control, and is optically clear, live imaging is a powerful tool in for this model system. Combining genetics with high-resolution imaging has continued to make important contributions to many fields. In this chapter, we discuss how certain aspects of high-resolution microscopy are implemented. This is not an exhaustive review of microscopy; it is meant to be a helpful guide and point of reference for some basic concepts in imaging. While these concepts are largely true for all biological imaging, they are chosen as particularly important for C. elegans.
Institute for Research in Immunology and Cancer (IRIC), Department of Pathology and Cell Biology, Université de Montréal, P.O. Box 6128, Station Centre-Ville, Montréal Quebec, Canada.
Amy S
AS
Maddox
Paul S
PS
Maddox
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000648:ultrastructure*; D003199:Computer Systems; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000502:physiology*; D005404:Fixatives; D049452:Green Fluorescent Proteins; D007814:Larva / Q000502:physiology / Q000648:ultrastructure*; D008853:Microscopy; D018613:Microscopy, Confocal; D008954:Models, Biological; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*
D005404:Fixatives; D049452:Green Fluorescent Proteins