The structural basis of the response regulator DrRRA from Deinococcus radiodurans.
DrRRA, a vital and recently discovered gene product of Deinococcus radiodurans, is a member of the OmpR/PhoB family of response regulators that couple with the cognate histidine kinase (HK) to form a typical two component system (TCS). It is known that the DrRRA is responsible for the transcriptional levels of numerous genes mostly relating to the stress response and DNA repair. In this paper, the crystal structures of the effector domain and full-length protein of DrRRA with resolutions of 1.6 and 2.3Å, respectively, are determined. These crystal structures depicted that DrRRA has the structural features of the OmpR/PhoB subfamily and were also confirmed by SAXS investigation of the protein in solution. Our data suggest that the receiver domain blocks the binding of DNA to the DNA recognition helix of effector domain; while the interdomain interface would be unwrapped, via the phosphorylation of receiver domain and/or the inducement of DNA, in order to provide DNA binding.
Center for Multi-disciplinary Research, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Ying
Y
Liu
Zeng-Qiang
ZQ
Gao
Zhun
Z
She
Kun
K
Qu
Wen-Jia
WJ
Wang
Eleonora V
EV
Shtykova
Jian-Hua
JH
Xu
Chao-Neng
CN
Ji
Yu-Hui
YH
Dong
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D034301:Deinococcus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D053838:Scattering, Small Angle; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; C061278:PhoB protein, Bacteria; D004247:DNA