Micronucleated erythrocytes in preterm newborns exposed to phototherapy and/or oxygentherapy.
Preterm newborns (PNBs) have an immature antioxidant defense system, and this makes them more susceptible to oxidative stress generated by postnatal treatments. The objective was to determine whether micronucleated erythrocytes increase in PNB by postnatal treatments such as oxygentherapy and phototherapy. We counted micronucleated erythrocytes and micronucleated polychromatic erythrocytes as DNA damage in 72 blood samples of PNB at 26-36 weeks of gestation, taken between 1 and 84 h after birth. We assume that more time passed between sampling and birth would correspond to greater time of exposure to oxygen (37 cases) and phototherapy plus oxygen (35 cases). In the PNB only exposed to oxygen, the differences were not significant, while there was a significant increase in micronucleated polychromatic erythrocytes with increasing exposure time in those treated with phototherapy plus oxygen. In conclusion, our results suggest that the MN increase from phototherapy can be observed in peripheral blood erythrocytes of PNB.
Laboratorio de Mutagénesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico. mutagenesis95@hotmail.com
Guillermo M
GM
Zúñiga-González
Belinda C
BC
Gómez-Meda
María de Lourdes
Mde L
Lemus-Varela
Ana L
AL
Zamora-Perez
Juan
J
Armendáriz-Borunda
Angélica
A
Barros-Hernández
Adriana
A
Sánchez-Díaz
Martha P
MP
Gallegos-Arreola
D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010100:Oxygen / Q000009:adverse effects* / Q000627:therapeutic use*; D010789:Phototherapy / Q000009:adverse effects*; D047928:Premature Birth / Q000097:blood* / Q000235:genetics / Q000378:metabolism / Q000628:therapy*
D010100:Oxygen