Prevalence of the insulin receptor substrate-1(IRS-1) Gly972Arg and the insulin receptor substrate-2(IRS-2) Gly1057Asp polymorphisms in PCOS patients and non-diabetic healthy women.
OBJECTIVE
The aim of our study was to investigate the frequency and compare the prevalence of IRS-1Gly972Arg and IRS-2 Gly1057Asp polymorphisms in PCOS patients and non-diabetic healthy women.                                               MATERIAL(S) AND METHOD(S):                          Forty eight Iranian women diagnosed with PCOS were enrolled in this study. Fifty two non-diabetics, non-PCOS women were enrolled as the control group. HemoglobinA1c (HbA1c), fasting blood glucose (FBS), fasting insulin levels (FIL) and 2 h post-prandial blood glucose(2hpp BS) were evaluated from blood samples. Insulin resistance sample was estimated with Homeostasis Model Assessment index for insulin resistance (HOMA-IR). Genotyping of allelic variants Gly972Arg of IRS-1 and Gly1057Asp of IRS-2 was conducted using PCR.


RESULTS
No statistically significant differences in the prevalence of IRS-1 Gly972Arg or IRS-2 Gly1057Asp polymorphisms or any combination of both were observed between controls and PCOS patients (P > 0.02). Control subjects with the IRS-1 polymorphism had higher levels of 2hpp BS compared with those with the Gly/Gly genotype (P = 0.037).


CONCLUSIONS
Considering that no association between the IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms and PCOS were found, the results confirm that these polymorphisms should not be considered as major contributors to the pathogenesis of this disorder.
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran. bhrashidi@Tums.ac.ir
Batool
B
Rashidi
Leila
L
Azizy
Farhad
F
Najmeddin
Ebrahim
E
Azizi
D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D001786:Blood Glucose; D015992:Body Mass Index; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D007333:Insulin Resistance / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000473:pathology; D011110:Polymorphism, Genetic
D001786:Blood Glucose; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins