Isolation and characterization of vascular endothelial cells from murine heart and lung.
The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.
Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Healthy Science Center, Temple, TX, USA.
Yixin
Y
Jin
Yang
Y
Liu
Marc
M
Antonyak
Xu
X
Peng
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D004210:Dissection; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D005780:Gelatin / Q000737:chemistry; D055786:Gene Knockout Techniques; D008168:Lung / Q000166:cytology*; D059346:Magnets / Q000737:chemistry; D051379:Mice; D008822:Mice, Transgenic; D008863:Microspheres; D009206:Myocardium / Q000166:cytology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D015398:Signal Transduction
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; C480027:ICAM-2 protein, mouse; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D005780:Gelatin