A novel click lysine zwitterionic stationary phase for hydrophilic interaction liquid chromatography.
A novel type of zwitterionic HILIC stationary phase was prepared by covalently bonding the l-azido lysine on silica gel via click chemistry. The key intermediate azido lysine was synthesized by transformation the amino group in l-Boc-lysine to corresponding azido group and subsequent removal of the N-protected group (Boc). Finally, the azido lysine was covalently bonded to silica beads by click chemistry to get click lysine. Its structure was confirmed by FT-IR and elemental analysis. The new stationary phase showed good HILIC characteristics, when it was applied to separate polar and hydrophilic compounds, such as organic acids, cephalosporins and carbapenems. Compared with the commercial stationary phases, such as Atlantics HILIC and ZIC-HILIC, click lysine displayed better or similar chromatographic behaviors.
School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Hongyue
H
Guo
Renhua
R
Liu
Jinjin
J
Yang
Bingcheng
B
Yang
Xinmiao
X
Liang
Changhu
C
Chu
D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D057930:Click Chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D058428:Silica Gel / Q000138:chemical synthesis / Q000737:chemistry*
D001386:Azides; D058428:Silica Gel; D008239:Lysine