Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.
Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage.
State Key Laboratory of Cell Biology, Shanghai Key Laboratory for Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Sheng-Nan
SN
Hu
Hui
H
Yu
Yun-Bin
YB
Zhang
Zhi-Li
ZL
Wu
Yuan-Chang
YC
Yan
Yi-Xue
YX
Li
Yuan-Yuan
YY
Li
Yi-Ping
YP
Li
D017398:Alternative Splicing / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D036703:Blastula / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D060172:Morpholinos / Q000494:pharmacology; D009035:Mothers; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055747:SOX Transcription Factors / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics*; D015053:Zygote / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D060172:Morpholinos; D012333:RNA, Messenger; D055747:SOX Transcription Factors; D029961:Zebrafish Proteins; C420982:sox19b protein, zebrafish