[Establishment of a sandwich ELISA for testing serum sB7-H3 and detection of sB7-H3 levels in liver disease].
OBJECTIVE
To establish quantitative ELISA for soluble sB7-H3 and evaluate its clinical application.


METHODS
Two mAbs of mouse anti-human sB7-H3(4H7 and 2E6)established by our lab were used. The mAb 4H7 was used as coating antibody and the 2E6 as a sandwich antibody,  which recognized a different epitope and was labeled by biotin. The serum sB7-H3 levels of healthy volunteers and patients were measured by this method.


RESULTS
Sandwich ELISA was established with the linear range covering from 8.192 ng/L to 2 000 ng/L. The outcomes of repeatability test,  recovery test,  stability test and specificity test were quite good. The increased levels of sB7-H3 were found in schistosomiasis cirrhosis,  the reduced levels in severe hepatitis and the difference in both situations showed significance.


CONCLUSIONS
A specific and sensitive sandwich ELISA is developed,  the detection in liver disease indicates that the ELISA kit provides a potential tool in the clinical application.
Medical Biotechnology Institute of Soochow University,  Suzhou  215007, China.
Shu-xiang
SX
Li
Guang-bo
GB
Zhang
Hai-hong
HH
Sun
Xin
X
Gao
Xue-guang
XG
Zhang
D000911:Antibodies, Monoclonal / Q000276:immunology; D060887:B7 Antigens / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D060887:B7 Antigens; C423237:CD276 protein, human