Structural analysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei.
Capsular polysaccharides (CPSs) were isolated from O-polysaccharide deficient strains of Burkholderia mallei and Burkholderia pseudomallei using a modified hot phenol/water extraction procedure. Glycosyl composition, methylation, MALDI-TOF MS analyses as well as (1)H NMR spectroscopy including COSY, TOCSY, NOESY, HMBC and HSQC experiments identified the presence of two distinct CPS antigens in the samples exhibiting the following structures: This study confirms the ability of B. mallei to express a 6-deoxy-heptan CPS and represents the first report of a mannan CPS being expressed by these bacterial pathogens.
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA. cheiss@ccrc.uga.edu
Christian
C
Heiss
Mary N
MN
Burtnick
Zhirui
Z
Wang
Parastoo
P
Azadi
Paul J
PJ
Brett
D016667:Bacterial Capsules / Q000737:chemistry / Q000235:genetics*; D042726:Burkholderia mallei / Q000235:genetics* / Q000254:growth & development; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000254:growth & development; D002236:Carbohydrate Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011134:Polysaccharides / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification
D011134:Polysaccharides