Partial excision of blaKPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae.
We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla(KPC) fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla(KPC) is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.
Public Health Research Institute Tuberculosis Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.
Liang
L
Chen
Kalyan D
KD
Chavda
José R
JR
Mediavilla
Michael R
MR
Jacobs
Michael H
MH
Levi
Robert A
RA
Bonomo
Barry N
BN
Kreiswirth
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases