Comparison of heat transfer in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation.
Slush nitrogen (SN(2)) is a mixture of solid nitrogen and liquid nitrogen, with an average temperature of -207 °C. To investigate whether plunging a French plastic straw (commonly used for sperm cryopreservation) in SN(2) substantially increases cooling rates with respect to liquid nitrogen (LN(2)), a numerical simulation of the heat conduction equation with convective boundary condition was used to predict cooling rates. Calculations performed using heat transfer coefficients in the range of film boiling confirmed the main benefit of plunging a straw in slush over LN(2) did not arise from their temperature difference (-207 vs. -196 °C), but rather from an increase in the external heat transfer coefficient. Numerical simulations using high heat transfer (h) coefficients (assumed to prevail in SN(2)) suggested that plunging in SN(2) would increase cooling rates of French straw. This increase of cooling rates was attributed to a less or null film boiling responsible for low heat transfer coefficients in liquid nitrogen when the straw is placed in the solid-liquid mixture or slush. In addition, predicted cooling rates of French straws in SN(2) tended to level-off for high h values, suggesting heat transfer was dictated by heat conduction within the liquid filled plastic straw.
Facultad de Ciencias Agrarias, Pontificia Universidad Católica, Argentina, Cap. Gral. Ramón Freire 183, 1426 Buenos Aires, Argentina. marina.sansinena@gmail.com
M
M
Sansinena
M V
MV
Santos
N
N
Zaritzky
J
J
Chirife
D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D006358:Hot Temperature; D008297:Male; D009584:Nitrogen; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature
D009584:Nitrogen