A secreted placental alkaline phosphatase-based reporter assay system for screening of compounds acting at an octopamine receptor stably expressed in a mammalian cell line.
Octopamine receptors are attractive insecticide targets. To screen compounds acting at octopamine receptors simply and rapidly, we constructed a chemiluminescent reporter gene assay system that detects secreted placental alkaline phosphatase transcriptionally regulated by the cAMP response element for a silkworm octopamine receptor. This system proved useful in high-throughput screening to develop octopamine receptor-specific insecticides.
Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto, Japan. hiohta@gpo.kumamoto-u.ac.jp
Hiroto
H
Ohta
Hiroshi
H
Oshiumi
Naotaka
N
Hayashi
Tetsuya
T
Imai
Yoshihisa
Y
Ozoe
Shigeru
S
Morimura
Kenji
K
Kida
D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D017982:Receptors, Biogenic Amine / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*
D007306:Insecticides; D017982:Receptors, Biogenic Amine; C027604:norsynephrine receptor; D000469:Alkaline Phosphatase