A validated high-performance liquid chromatographic method with diode-array detection for the estimation of xyloketal B in rat plasma.
A sensitive and specific HPLC-UV method was developed and validated for the determination of xyloketal B in rat plasma. Following liquid-liquid extraction, the separation was performed using an isocratic mobile phase of methanol-acetonitrile-water (30/30/40, v/v/v) on a Phenomenex C(18) column (4.6mm×250mm, 5μm). The eluent was monitored at 220nm and at a flow rate of 0.8mlmin(-1). A linear curve over the concentration range of 1-128μg/ml (r>0.999) was established. The LLOQ of the method was 1μg/ml. Good precision and accuracy at concentrations of 2.5, 25 and 100μg/ml were obtained. The recovery of xyloketal B in plasma was >87.91%. The validated method was found to be specific, precise and accurate in the study. The analytic method was satisfactorily applied to perform preclinical pharmacokinetic study of xyloketal B in rat plasma.
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China.
Wei
W
Zhang
Yan
Y
Liu
Hongtu
H
Yang
Zongyi
Z
Li
Yadong
Y
Huang
Zhongliang
Z
Xu
Yongcheng
Y
Lin
Qi
Q
Xiang
Jiyan
J
Pang
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D059625:Liquid-Liquid Extraction; D008297:Male; D011714:Pyrans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011714:Pyrans; C515343:xyloketal B