Genotyping of hepatitis C virus isolated from hepatitis patients in Southeast of Iran by Taqman Realtime PCR.
OBJECTIVE
To check TaqMan Realtime PCR in detecting genotypes of hepatitis C virus in Iran.


METHODS
From July 2007 to April 2009, HCV genotyping was done on 52 patients who were referred to Research Centre for infectious Disease and Tropical Medicine, in Bou-Ali Hospital, Zahedan University of Medical Sciences. All these patients had proven hepatitis C infection.


RESULTS
Out of 52 anti HCV positive samples, 28 (53.84%) had genotype 1, 2 cases (3.88%) had genotype 2, 12 (23.08%) had genotype 3 and 7 (13.4%) had genotype 4. Mixed infection with genotypes 1 and 3 was seen in 3 cases (5.77%).


CONCLUSIONS
TaqMan probes for detecting genotyping of HCV were successful in picking genotyping of HCV infection especially those with mixed genotypes.
Cell and Molecular Research Institute, Department of Microbiology, School of Medicine, Qazvin University of Medical Sciences, Iran.
Taghi Naserpour
TN
Farivar
Mohammad Kazem
MK
Nezam
Pouran
P
Johari
D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D012367:RNA, Viral / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
D012367:RNA, Viral; D019914:Taq Polymerase