Microsatellite markers for the Chinese endangered and endemic orchid Cymbidium tortisepalum (Orchidaceae).
OBJECTIVE
Microsatellite primers were developed in the Chinese endangered and endemic orchid, Cymbidium tortisepalum, to investigate its genetic diversity and population genetic structure, and to identify its varieties.


RESULTS
Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 15 primer sets were identified in two wild populations. The number of alleles per locus ranged from two to six, with a mean of 3.5. The observed and expected heterozygosities varied from 0.250 to 0.917 and from 0.228 to 0.841, respectively. All of these primers successfully amplified in the congener C. goeringii, and 12 were found useful in C. faberi and C. sinense.


CONCLUSIONS
These markers will facilitate further studies on the population genetics and molecular identification of C. tortisepalum, its varieties, and other congener species.
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.
Min
M
Tang
Chun-Xia
CX
Zeng
Yu-Fen
YF
Bi
Jun-Bo
JB
Yang
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056727:Endangered Species; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers