Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.).
Tor tor is an important game and food fish of India with a distribution throughout Asia from the trans-Himalayan region to the Mekong River basin to Malaysia, Pakistan, Bangladesh and Indonesia. A new cell line named TTCF was developed from the caudal fin of T. tor for the first time. The cell line was optimally maintained at 28°C in Leibovitz-15 (L-15) medium supplemented with 20% fetal bovine serum (FBS). The propagation of TTCF cells showed a high plating efficiency of 63.00%. The cytogenetic analysis revealed a diploid count of 100 chromosomes at passage 15, 30, 45 and 60 passages. The viability of the TTCF cell line was found to be 72% after 6 months of cryopreservation in liquid nitrogen (-196°C). The origin of the cell lines was confirmed by the amplification of 578- and 655-bp sequences of 16S rRNA and cytochrome oxidase subunit I (COI) genes of mitochondrial DNA (mtDNA) respectively. TTCF cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids. Further, immunocytochemistry studies confirm its fibroblastic morphology of cells. Genotoxicity assessment of H₂O₂ in TTCF cell line revealed the utility of TTCF cell line as in vitro model for aquatic toxicological studies.
National Bureau of Fish Genetic Resources (NBFGR), Lucknow, UP, 226002, India. kamal_biotec@yahoo.com.
K
K
Yadav
Central Institute of Fisheries Education, Versova, Andheri (W), Mumbai, 400061, India.
W S
WS
Lakra
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
J
J
Sharma
National Bureau of Fish Genetic Resources (NBFGR), Lucknow, UP, 226002, India.
M
M
Goswami
National Bureau of Fish Genetic Resources (NBFGR), Lucknow, UP, 226002, India.
Akhilesh
A
Singh
D000818:Animals; D002460:Cell Line / Q000166:cytology*; D049109:Cell Proliferation; D020552:Comet Assay; D015925:Cryopreservation; D003530:Cyprinidae; D056727:Endangered Species; D017930:Genes, Reporter; D007150:Immunohistochemistry; D007621:Karyotyping