The porcine Gpr3 gene: molecular cloning, characterization and expression level in tissues and cumulus-oocyte complexes during in vitro maturation.
G protein-coupled receptor 3 (Gpr3) is a member of G protein-coupled receptor rhodopsin family, which is present throughout the follicle within the ovary and functions as a critical factor for the maintenance of meiotic prophase arrest in oocytes by a Gs protein-mediated pathway. In the current paper, attempts were made to clone and characterize a gene encoding Gpr3 from pigs and investigate its expression pattern in tissues and the whole cumulus-oocyte complexes (COCs) in vitro maturation (IVM). Rapid amplification of cDNA ends and RT-PCR gave rise to the full sequence of Gpr3 gene with its length being 2101 bp nucleotides, including an open reading frame of 993 bp, encoding a 331 amino acid polypeptide with the molecular weight of 35.2 kDa. Homology search and sequence multi-alignment demonstrated that the putative porcine Gpr3 protein sequence shared a high identity with other animal Gpr3 orthologs, including several highly conservative motifs and amino acids. Real-time PCR analysis showed that the Gpr3 gene was expressed in tissues of cerebrum, cerebellum, hypothalamus, pituitary, ovary, oviduct, uterus, heart, liver, spleen, lung, kidney, muscle, fat, testis, thymus and granulosa cell, oocyte and COCs at different expression levels. The expression levels of this gene in oocyte, uterus, liver, fat, pituitary and brain were higher than that in other tissues. Interestingly, the mRNA and protein levels of Gpr3 in the whole COCs were down-regulated, and its mRNA expression levels were significantly and negatively correlated with the degrees of cumulus expansion (r = -0.937, P < 0.01) during IVM, suggesting its important roles in cumulus expansion and oocyte maturation.
College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China.
Baole
B
Zhang
Jianhua
J
Ding
Ye
Y
Li
Jingjing
J
Wang
Yongyan
Y
Zhao
Wei
W
Wang
Shuai
S
Shi
Fulu
F
Dong
Zijing
Z
Zhang
Fangxiong
F
Shi
Yinxue
Y
Xu
D000818:Animals; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D048430:Cell Shape; D003001:Cloning, Molecular; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*; D014018:Tissue Distribution
D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled