Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters.
In this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. Candida rugosa lipase (CRL) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. The resulting immobilized CRL increased remarkably its stability at high temperature in comparison to free CRL. The immobilized CRL was used as biocatalysts for enzymatic esterification of phytosterols with free fatty acids (FFAs) to produce phytosterol esters. The phytosterols linolenate esterification degree of 95.3% was obtained under the optimized condition. Phytosterols esters could also been converted in high yields to the corresponding long-chain acyl esters via transesterification with methyl esters of fatty acids (80.5%) or triacylglycerols (above 95.5%) using mixed-mode silica particles immobilized CRL as biocatalyst. Furthermore, the immobilized CRL by absorption retained 78.6% of their initial activity after 7 recycles.
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture Key Laboratory of Oil Crops Biology, Wuhan 430062, China.
Ming-Ming
MM
Zheng
Yong
Y
Lu
Ling
L
Dong
Ping-Mei
PM
Guo
Qian-Chun
QC
Deng
Wen-Lin
WL
Li
Yu-Qi
YQ
Feng
Feng-Hong
FH
Huang
D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology*; D002411:Cation Exchange Resins / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D004952:Esters / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000378:metabolism*; D008863:Microspheres; D010840:Phytosterols / Q000378:metabolism*; D059027:Recycling; D012822:Silicon Dioxide / Q000737:chemistry*; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D017962:alpha-Linolenic Acid / Q000378:metabolism
D002411:Cation Exchange Resins; D004800:Enzymes, Immobilized; D004952:Esters; D010840:Phytosterols; D013451:Sulfonic Acids; D017962:alpha-Linolenic Acid; D012822:Silicon Dioxide; D008049:Lipase