Quantitative determination of sauchinone in rat plasma by liquid chromatography-mass spectrometry.
A rapid, sensitive and specific method using liquid chromatography with tandem mass spectrometric detection (LC-MS) was developed for the analysis of sauchinone in rat plasma. Di-O-methyltetrahydrofuriguaiacin B was used as internal standard (IS). Analytes were extracted from rat plasma by liquid-liquid extraction using ethyl acetate. A 2.1 mm i.d. × 150 mm, 5 µm, Agilent Zorbax SB-C(18) column was used to perform the chromatographic analysis. The mobile phase was methanol-deionized water (80:20, v/v). The chromatographic run time was 7 min per injection and the flow-rate was 0.2 mL/min. The tandem mass spectrometric detection mode was achieved with electrospray ionization interface in positive-ion mode (ESI(+) ). The m/z ratios [M + Na](+) , m/z 379.4 for sauchinone and m/z 395.4 for IS were recorded simultaneously. Calibration curve were linear over the range of 0.01-5 µg/mL. The lowest limit of quantification was 0.01 µg/mL. The intra-day and inter-day precision and accuracy of the quality control samples were 2.94-9.42% and 95.79-108.05%, respectively. The matrix effect was 64.20-67.34% and the extraction recovery was 93.28-95.98%. This method was simple and sensitive enough to be used in pharmacokinetic research for determination of sauchinone in rat plasma.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
Chun-lei
CL
Xu
Jian-wei
JW
Chen
Wen-zheng
WZ
Ju
Shi-jia
SJ
Liu
Yong
Y
Chen
Zhi-peng
ZP
Chen
Ping
P
Xue
Hong-jiang
HJ
Chen
Xiang
X
Li
D000818:Animals; D001578:Benzopyrans / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004149:Dioxoles / Q000097:blood* / Q000737:chemistry; D004355:Drug Stability; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D001578:Benzopyrans; D004149:Dioxoles; C410958:sauchinone