Whole-mount confocal microscopy for vascular branching morphogenesis.
We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.
Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. mukoyamay@mail.nih.gov
Yoh-suke
YS
Mukouyama
Jennifer
J
James
Joseph
J
Nam
Yutaka
Y
Uchida
D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000196:embryology*; D006224:Cricetinae; D042601:Lymphatic Vessels / Q000166:cytology / Q000196:embryology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D009206:Myocardium / Q000166:cytology; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000098:blood supply; D013048:Specimen Handling; D013194:Staining and Labeling