Determination of γ-hydroxybutyrate (GHB), β-hydroxybutyrate (BHB), pregabalin, 1,4-butane-diol (1,4BD) and γ-butyrolactone (GBL) in whole blood and urine samples by UPLC-MSMS.
The demand of high throughput methods for the determination of gamma-hydroxybutyrate (GHB) and its precursors gamma-butyrolactone (GBL) and 1,4-butane-diol (1,4BD) as well as for pregabalin is increasing. Here we present two analytical methods using ultra-high pressure liquid chromatography (UPLC) and tandem mass spectrometric (MS/MS) detection for the determination of GHB, beta-hydroxybutyrate (BHB), pregabalin, 1,4BD and GBL in whole blood and urine. Using the 96-well formate, the whole blood method is a simple high-throughput method suitable for screening of large sample amounts. With an easy sample preparation for urine including only dilution and filtration of the sample, the method is suitable for fast screening of urine samples. Both methods showed acceptable linearity, acceptable limits of detection, and limits of quantification. The within-day and between-day precisions of all analytes were lower than 10% RSD. The analytes were extracted from matrices with recoveries near 100%, and no major matrix effects were observed. Both methods have been used as routine screening analyses of whole blood and urine samples since January 2010.
Norwegian Institute of Public Health, Division of Forensic Medicine and Drug Abuse Research, P.O. Box 4404, Nydalen, 0403 Oslo, Norway. sandrari@kjemi.uio.no
Sandra Rinne
SR
Dahl
Kirsten Midtbøen
KM
Olsen
Dag Helge
DH
Strand
D020155:3-Hydroxybutyric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D015107:4-Butyrolactone / Q000032:analysis* / Q000097:blood / Q000652:urine; D002072:Butylene Glycols / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000097:blood / Q000652:urine; D000069583:Pregabalin; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012978:Sodium Oxybate / Q000032:analysis* / Q000097:blood / Q000652:urine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine
D002072:Butylene Glycols; D013287:Illicit Drugs; D000069583:Pregabalin; D005680:gamma-Aminobutyric Acid; D012978:Sodium Oxybate; C039681:1,4-butanediol; D015107:4-Butyrolactone; D020155:3-Hydroxybutyric Acid