Microsatellite markers derived from Calophyllum inophyllum (Clusiaceae) expressed sequence tags.
OBJECTIVE
Robust markers are required (inter alia) for assessing origins of Calophyllum inophyllum populations on the Bonin Islands, Japan. Therefore, informative expressed sequence tag (EST)-based microsatellite or simple sequence repeat (SSRs) markers in the species were sought.


RESULTS
Using 135378 ESTs derived from de novo pyrosequencing, primers for 475 EST-SSRs were developed, 48 of which were tested for PCR amplification. Thirty-six of the 48 primers showed clear amplification, with 23 displaying polymorphism in sampled populations. Expected heterozygosity in the samples from the Bonin Islands and Ryukyu Islands populations ranged from 0.041 to 0.697 and from 0.041 to 0.773, respectively.


CONCLUSIONS
As EST-SSRs are potentially tightly linked with functional genes, and reportedly more transferable to related species than anonymous genomic SSRs, the developed primers have utility for future studies of the origins, genetic structure, and conservation of C. inophyllum and related species.
Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan. setsukos@affrc.go.jp
Suzuki
S
Setsuko
Kentaro
K
Uchiyama
Kyoko
K
Sugai
So
S
Hanaoka
Hiroshi
H
Yoshimaru
D000483:Alleles; D001483:Base Sequence; D029759:Calophyllum / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D058974:Phylogeography; D011110:Polymorphism, Genetic; D036226:Seedlings / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers