Software for precise tracking of cell proliferation.
We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to adoption of backward tracing and addition of user-interactive correction functions, TADOR is used in an off-line and semi-automated mode, but enables precise tracking of cell division. By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time.
Brain Science Institute, RIKEN, Wako-city, Saitama, Japan.
Hiroshi
H
Kurokawa
Hisayori
H
Noda
Mayu
M
Sugiyama
Asako
A
Sakaue-Sawano
Kiyoko
K
Fukami
Atsushi
A
Miyawaki
D002453:Cell Cycle; D002455:Cell Division; D049109:Cell Proliferation; D058948:Cell Tracking / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software
D005456:Fluorescent Dyes