Effect of nanocoating with rhamnogalacturonan-I on surface properties and osteoblasts response.
Long-term stability of titanium implants are dependent on a variety of factors. Nanocoating with organic molecules is one of the methods used to improve osseointegration. Therefore, the aim of this study is to evaluate the in vitro effect of nanocoating with pectic rhamnogalacturonan-I (RG-I) on surface properties and osteoblasts response. Three different RG-Is from apple and lupin pectins were modified and coated on amino-functionalized tissue culture polystyrene plates (aminated TCPS). Surface properties were evaluated by scanning electron microscopy, contact angle measurement, atomic force microscopy, and X-ray photoelectron spectroscopy. The effects of nanocoating on proliferation, matrix formation and mineralization, and expression of genes (real-time PCR) related to osteoblast differentiation and activity were tested using human osteoblast-like SaOS-2 cells. It was shown that RG-I coatings affected the surface properties. All three RG-I induced bone matrix formation and mineralization, which was also supported by the finding that gene expression levels of alkaline phosphatase, osteocalcin, and collagen type-1 were increased in cells cultured on the RG-I coated surface, indicating a more differentiated osteoblastic phenotype. This makes RG-I coating a promising and novel candidate for nanocoatings of implants.
Research Center for Ageing and Osteoporosis, Departments of Medicine and Clinical Biochemistry, Copenhagen University Hospital Glostrup, Ndr. Ringvej 69, 2600 Glostrup, Denmark. kagu@sund.ku.dk
Katarzyna
K
Gurzawska
Rikke
R
Svava
Susanne
S
Syberg
Yu
Y
Yihua
Kenneth Brian
KB
Haugshøj
Iben
I
Damager
Peter
P
Ulvskov
Leif Højslet
LH
Christensen
Klaus
K
Gotfredsen
Niklas Rye
NR
Jørgensen
D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D031297:Lupinus / Q000737:chemistry; D027845:Malus / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; C042491:rhamnogalacturonan I; D010368:Pectins