Exploring the application of high-throughput genomics technologies in the field of maternal-embryo communication.
Deciphering the complex molecular dialogue between the maternal tract and embryo is crucial to increasing our understanding of pregnancy failure, infertility problems and in the modulation of embryo development, which has consequences through adulthood. High-throughput genomic technologies have been applied to look for a holistic view of the molecular interactions occurring during this dialogue. Among these technologies, microarrays have been widely used, being one of the most popular tools in maternal-embryo communication. Today, next generation sequencing technologies are dwarfing the capabilities of microarrays. The application of these new technologies has broadened to almost all areas of genomics research, because of their massive sequencing capacity. We review the current status of high-throughput genomic technologies and their application to maternal-embryo communication research. We also survey next generation technologies and their huge potential in many research areas. Given the diversity of unanswered questions in the field of maternal-embryo communication and the wide range of possibilities that these technologies offer, here we discuss future perspectives on the use of these technologies to enhance maternal-embryo research.
Academic Unit of Reproductive and Development Medicine, University of Sheffield, Sheffield, UK. calmi@um.es
Carmen
C
Almiñana
Alireza
A
Fazeli
D000818:Animals; D018076:DNA, Complementary; D047108:Embryonic Development / Q000235:genetics* / Q000502:physiology; D005260:Female; D015870:Gene Expression; D005836:Genitalia, Female; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D008431:Maternal-Fetal Exchange; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000235:genetics* / Q000502:physiology; D012313:RNA / Q000032:analysis; D017421:Sequence Analysis
D018076:DNA, Complementary; D012313:RNA