Nocardioides lianchengensis sp. nov., an actinomycete isolated from soil.
A novel Gram-stain-positive, aerobic, rod-shaped bacterium, designated strain D94-1(T), was isolated from soil collected in Liancheng county, Fujian province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain D94-1(T) was a member of the genus Nocardioides and was most closely related to Nocardioides salarius DSM 18239(T) (98.54% 16S rRNA gene sequence similarity), Nocardioides marinisabuli DSM 18965(T) (98.30%), Nocardioides basaltis KCTC 19365(T) (98.10%) and Nocardioides dokdonensis KCTC 19309(T) (97.76%). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain D94-1(T) from N. salarius, N. marinisabuli, N. basaltis and N. dokdonensis. The chemotaxonomic properties of strain D94-1(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16:0) (29.15%) and anteiso-C(17:0) (21.0%). The DNA G+C content was 71.8 mol%. On the basis of the evidence presented in this study, strain D94-1(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides lianchengensis sp. nov. is proposed. The type strain is D94-1(T) (=DSM 24663(T)=CGMCC 4.6858(T)).
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, PR China.
Jian
J
Zhang
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.|College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, PR China.
Yuchao
Y
Ma
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China.
Huimin
H
Yu
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2