[Analytical method of cereulide in processed cereal-based foods and milk powder by LC-MS/MS].
An LC-MS/MS method for analysis of cereulide, an emetic toxin produced by Bacillus cereus, was developed. Cereulide was extracted from samples, fried rice, pan-fried noodles, red bean paste and baby formula, with methanol and purified using Oasis HLB cartridges. LC separation was performed on a C18 column with a mixture of formic acid solution and methanol containing ammonium formate as a mobile phase, and the mass spectrometer was operated in the positive electrospray ionization mode. Performance evaluation showed that trueness was higher than 70% and repeatability and reproducibility were within 10%. The limits of quantification were lower than 1 µg/kg.
Saito Laboratory, Japan Food Research Laboratories, Ibaraki-shi, Osaka, Japan. yatsukaway@jfrl.or.jp
Yoh-ichi
Y
Yatsukawa
Hanako
H
Iida
Kazuko
K
Nagata
Masashi
M
Miyamoto
Takahiro
T
Matsuda
Hironobu
H
Ito
Munetomo
M
Nakamura
Kazuhiro
K
Fujita
D000818:Animals; D001409:Bacillus cereus / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D047630:Depsipeptides / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D002523:Edible Grain / Q000737:chemistry* / Q000382:microbiology; D005504:Food Analysis / Q000379:methods*; D005516:Food Microbiology; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D011208:Powders; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D047630:Depsipeptides; D011208:Powders; C089294:cereulide