Laser microsurgery in Caenorhabditis elegans.
Laser killing of cell nuclei has long been a powerful means of examining the roles of individual cells in C. elegans. Advances in genetics, laser technology, and imaging have further expanded the capabilities and usefulness of laser surgery. Here, we review the implementation and application of currently used methods for target edoptical disruption in C. elegans.
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
Christopher
C
Fang-Yen
Christopher V
CV
Gabel
Aravinthan D T
AD
Samuel
Cornelia I
CI
Bargmann
Leon
L
Avery
D000818:Animals; D019771:Axotomy; D017173:Caenorhabditis elegans / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D019070:Cell Lineage; D002467:Cell Nucleus / Q000528:radiation effects / Q000648:ultrastructure; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D007814:Larva / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D053685:Laser Therapy / Q000379:methods*; D007834:Lasers; D028022:Low-Level Light Therapy; D044085:Microfluidics; D008866:Microsurgery / Q000379:methods*; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects; D017319:Photosensitizing Agents
D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins