Mitochondrial oxidative stress and epilepsy in SOD2 deficient mice: attenuation by a lipophilic metalloporphyrin.
Epileptic seizures are a common feature associated with inherited mitochondrial diseases. This study investigated the role of mitochondrial oxidative stress in epilepsy resulting from mitochondrial dysfunction using cross-bred mutant mice lacking mitochondrial manganese superoxide dismutase (MnSOD or SOD2) and a lipophilic metalloporphyrin catalytic antioxidant. Video-EEG monitoring revealed that in the second to third week of postnatal life (P14-P21) B6D2F2 Sod2(-/-) mice exhibited frequent spontaneous motor seizures providing evidence that oxidative stress-induced mitochondrial dysfunction may contribute to epileptic seizures. To confirm the role of mitochondrial oxidative stress in epilepsy a newly developed lipophilic metalloporphyrin, AEOL 11207, with high potency for catalytic removal of endogenously generated reactive oxygen species was utilized. AEOL 11207-treated Sod2(-/-) mice showed a significant decrease in both the frequency and duration of spontaneous seizures but no effect on seizure severity. A significant increase in the average lifespan of AEOL 11207-treated Sod2(-/-) mice compared to vehicle-treated Sod2(-/-) mice was also observed. Indices of mitochondrial oxidative stress and damage (aconitase inactivation, 3-nitrotyrosine formation, and depletion of reduced coenzyme A) and ATP levels affecting neuronal excitability were significantly attenuated in the brains of AEOL 11207-treated Sod2(-/-) mice compared to vehicle-treated Sod2(-/-) mice. The occurrence of epileptic seizures in Sod2(-/-) mice and the ability of catalytic antioxidant therapy to attenuate seizure activity, mitochondrial dysfunction, and ATP levels suggest that ongoing mitochondrial oxidative stress can contribute to epilepsy associated with mitochondrial dysfunction and disease.
Department of Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO 80045, USA.
Li-Ping
LP
Liang
Simon
S
Waldbaum
Shane
S
Rowley
Ting-Ting
TT
Huang
Brian J
BJ
Day
Manisha
M
Patel
D000154:Aconitate Hydratase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000927:Anticonvulsants / Q000627:therapeutic use*; D002540:Cerebral Cortex / Q000473:pathology / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D003065:Coenzyme A / Q000378:metabolism; D004195:Disease Models, Animal; D004569:Electroencephalography; D004827:Epilepsy / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D005649:Fumarate Hydratase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D008665:Metalloporphyrins / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008928:Mitochondria / Q000187:drug effects*; D009243:NAD / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D013482:Superoxide Dismutase / Q000172:deficiency*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D000099156:Superoxide Dismutase 2
D000154:Aconitate Hydratase; D000227:Adenine Nucleotides; D000927:Anticonvulsants; D003065:Coenzyme A; D005649:Fumarate Hydratase; D005978:Glutathione; D019803:Glutathione Disulfide; D008665:Metalloporphyrins; D009243:NAD; D013482:Superoxide Dismutase; D014443:Tyrosine; D000099156:Superoxide Dismutase 2; C519387:AEOL 11207; C002744:3-nitrotyrosine