17-β estradiol in the acetylcholinesterase activity and lipid peroxidation in the brain and blood of ovariectomized adult and middle-aged rats.
OBJECTIVE
To investigate the 17-β estradiol in the acetylcholinesterase activity and lipid peroxidation in the brain and blood of ovariectomized rats of different ages.


METHODS
Animals were randomly assigned into three experimental groups of each age (n=6). Control groups consisted of adult (sham-A) and middle-aged (sham-MA) female rats, ovariectomized adult (OVX-A) and middle-aged (OVX-MA) rats without estrogen therapy reposition, and ovariectomized adult (OVX+E2-A) and middle-aged (OVX+E2-MA) rats treated with 17-β estradiol for 30days. After this period, AChE activity and lipid peroxidation were measured in the brain and blood.


RESULTS
The AChE activity increased (p<0.05) in striatum (ST) in OVX-A, OVX+E2-A and OVX-MA, and hippocampus (HP) in OVX-MA. The enzyme activity decreased (p<0.05) in ST of OVX+E2-MA, and cerebral cortex (CC) in OVX+E2-A, OVX-MA and OVX+E2-MA. Blood AChE activity increased (p<0.05) in OVX+E2-A and decreased (p<0.05) in OVX-MA. Lymphocyte AChE activity increased (p<0.05) in OVX-A and OVX+E2-A and decreased (p<0.05) in OVX-MA. Lipid peroxidation increased (p<0.05) in ST of OVX-A, CC of OVX-A and OVX-MA, HP of OVX-A, and cerebellum (CE) of OVX-A, OVX-MA, and OVX+E2-MA. Lipid peroxidation decreased (p<0.05) in ST, CC and CE of OVX+E2-A, and ST and HP of OVX+E2-MA. Similar values of lipid peroxidation to control groups were found in ST and HP of OVX-MA, HP of OVX+E2-A and CC of OVX+E2-MA.


CONCLUSIONS
17-β estradiol is able to modulate the AChE activity and non-neuronal cholinergic response as well as to reduce lipid peroxidation. Its response is dependent on the age and brain structure analyzed.
Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria, Brazil.
Danieli Brolo
DB
Martins
Cinthia Melazzo
CM
Mazzanti
Raqueli Teresinha
RT
França
Marciélen
M
Pagnoncelli
Márcio Machado
MM
Costa
Estevan Martins
EM
de Souza
Jamile
J
Gonçalves
Roselia
R
Spanevello
Roberta
R
Schmatz
Pauline
P
da Costa
Alexandre
A
Mazzanti
Diego Vilibaldo
DV
Beckmann
Marcelo da Silva
Mda S
Cecim
Maria Rosa
MR
Schetinger
Sonia Terezinha Dos Anjos
ST
Lopes
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D001921:Brain / Q000201:enzymology*; D004958:Estradiol / Q000008:administration & dosage*; D015914:Estrogen Replacement Therapy; D004967:Estrogens / Q000008:administration & dosage*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D008214:Lymphocytes / Q000201:enzymology*; D023421:Models, Animal; D009928:Organ Specificity; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D004967:Estrogens; D004958:Estradiol; D000110:Acetylcholinesterase