Induction of strong anti-HIV cellular immunity by a combination of Clostridium perfringens expressing HIV gag and virus like particles.
The lower gastrointestinal tract is a major mucosal site of HIV entry and initial infection. Thus, the induction of strong cellular immune responses at this mucosal site will be an important feature of an effective HIV vaccine. We have used a novel prime-boost vaccination approach to induce immune responses at mucosal sites. Orally delivered recombinant Clostridium perfringens expressing HIV-1 gag (Cp-Gag) was evaluated for induction of HIV-1 Gag specific T cell responses in a prime-boost model with intranasal inoculation of HIV-1 virus like particles (VLP). HIV-1 specific cellular immune responses in both the effector (Lamina propria) and inductive sites (Peyer's patches) of the gastrointestinal (GI) tract were significantly higher in mice immunized using Cp-Gag and VLPs in a prime-boost approach compared to mice immunized with either Cp-Gag or VLPs alone. Such cellular immune response was found to be mediated by both CD8(+) and CD4(+) T cells. Such a strong mucosal immune response could be very useful in developing a mucosal vaccine against HIV-1.
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, PA 15261, USA.
Poonam
P
Pegu
Ruth
R
Helmus
Phalguni
P
Gupta
Patrick
P
Tarwater
Lori
L
Caruso
Chengli
C
Shen
Ted
T
Ross
Yue
Y
Chen
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D003016:Clostridium perfringens / Q000276:immunology* / Q000378:metabolism; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000821:virology; D005822:Genetic Vectors / Q000008:administration & dosage / Q000276:immunology; D006678:HIV / Q000235:genetics / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D007111:Immunity, Cellular / Q000276:immunology*; D051379:Mice; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism
D016915:AIDS Vaccines; D054301:gag Gene Products, Human Immunodeficiency Virus