Genetic transformation of Salvia austriaca by Agrobacterium rhizogenes and diterpenoid isolation.
Hairy roots of Salvia austriaca Jacq. transformed with Agrobacterium rhizogenes strain A4 were obtained and transgenic status of the roots was confirmed by polymerase chain reaction (PCR) using rolB and rolC specific primers. The root cultures growing in half-strength Gamborg (1/2 B5) liquid medium supplemented with sucrose (30 g L(-1)) under light conditions (photoperiod: 16 h light/8 h dark) were examined for their ability to produce diterpenoids. From n-hexane extract the abietane-type diterpenoids royleanone, 15-deoxyfuerstione and taxodione were isolated and identified. This is the first report on the genetic transformation of S. austriaca.
Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Poland. lukikuzma@wp.pl
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Kuźma
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Kisiel
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Królicka
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Wysokińska
D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification; D060054:Agrobacterium / Q000235:genetics*; D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000737:chemistry; D017931:DNA Primers; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D017440:Photoperiod; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D027544:Salvia / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic / Q000235:genetics*
D045784:Abietanes; D003470:Culture Media; D017931:DNA Primers; D018744:DNA, Plant; D004224:Diterpenes; C103399:royleanone; C009387:taxodione