Proteomic analysis of Sulfolobus solfataricus during Sulfolobus Turreted Icosahedral Virus infection.
Where there is life, there are viruses. The impact of viruses on evolution, global nutrient cycling, and disease has driven research on their cellular and molecular biology. Knowledge exists for a wide range of viruses; however, a major exception are viruses with archaeal hosts. Archaeal virus-host systems are of great interest because they have similarities to both eukaryotic and bacterial systems and often live in extreme environments. Here we report the first proteomics-based experiments on archaeal host response to viral infection. Sulfolobus Turreted Icosahedral Virus (STIV) infection of Sulfolobus solfataricus P2 was studied using 1D and 2D differential gel electrophoresis (DIGE) to measure abundance and redox changes. Cysteine reactivity was measured using novel fluorescent zwitterionic chemical probes that, together with abundance changes, suggest that virus and host are both vying for control of redox status in the cells. Proteins from nearly 50% of the predicted viral open reading frames were found along with a new STIV protein with a homologue in STIV2. This study provides insight to features of viral replication novel to the archaea, makes strong connections to well-described mechanisms used by eukaryotic viruses such as ESCRT-III mediated transport, and emphasizes the complementary nature of different omics approaches.
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States.
Walid S
WS
Maaty
Kyla
K
Selvig
Stephanie
S
Ryder
Pavel
P
Tarlykov
Jonathan K
JK
Hilmer
Joshua
J
Heinemann
Joseph
J
Steffens
Jamie C
JC
Snyder
Alice C
AC
Ortmann
Navid
N
Movahed
Kevin
K
Spicka
Lakshindra
L
Chetia
Paul A
PA
Grieco
Edward A
EA
Dratz
Trevor
T
Douglas
Mark J
MJ
Young
Brian
B
Bothner
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D023641:Archaeal Viruses / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D040901:Proteomics / Q000379:methods*; D016415:Sequence Alignment; D048229:Sulfolobus solfataricus / Q000737:chemistry / Q000378:metabolism* / Q000821:virology*; D053719:Tandem Mass Spectrometry; D014779:Virus Replication
D019843:Archaeal Proteins