Fungal culture of musculoskeletal tissue: what's the point?
There have not been any studies that review the prevalence of fungal isolates using selective media from samples of banked musculoskeletal tissue retrieved from living and cadaveric donors. A total of 2,036 swab and 2,621 biopsy samples of musculoskeletal tissue from tissue banks were received from the 1st August 2008 till 31st December 2010. Routine culture for fungi using selective media with a prolonged incubation period failed to demonstrate a greater prevalence of fungal isolates than by using non-selective culture media alone. Using selective culture fungi were recovered from only two Sabouraud agar plates (0.1%) but not from non-selective media. During the same period fungi were isolated from three graft samples cultured in non-selective broth media only (0.1%). There was no correlation of fungal isolates from selective or non-selective media inoculated at the same time nor from multiple graft samples collected from the same donor supporting the possibility of an exogenous source for fungal isolates rather than an endogenous source.
Department of Microbiology, South Eastern Area Laboratory Services, St. George Hospital, Kogarah, NSW, Australia. Kerry.Varettas@sesiahs.health.nsw.gov.au
Kerry
K
Varettas
Peter
P
Taylor
D001232:Aspergillus fumigatus / Q000302:isolation & purification; D002102:Cadaver; D002176:Candida albicans / Q000302:isolation & purification; D041022:Candida tropicalis / Q000302:isolation & purification; D003470:Culture Media; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D009141:Musculoskeletal System / Q000382:microbiology*; D014015:Tissue Banks; D014019:Tissue Donors; D019737:Transplants / Q000382:microbiology*
D003470:Culture Media