Ingestion of proteoglycan fraction from shark cartilage increases serum inhibitory activity against matrix metalloproteinase-9 and suppresses development of N-nitrosobis(2-oxopropyl)amine-induced pancreatic duct carcinogenesis in hamster.
A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster.
Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Shimogamo, Kyoto, Japan.
Tsukasa
T
Kitahashi
Shoko
S
Ikawa
Akika
A
Sakamoto
Yoshihiro
Y
Nomura
Toshifumi
T
Tsujiuchi
Kenji
K
Shimizu
Shuji
S
Sasabe
Eun Young
EY
Park
Yasushi
Y
Nakamura
Masahiro
M
Tsutsumi
Kenji
K
Sato
D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000097:blood; D005260:Female; D061965:Matrix Metalloproteinase Inhibitors; D008647:Mesocricetus; D009602:Nitrosamines; D010183:Pancreatic Ducts; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D011509:Proteoglycans / Q000008:administration & dosage*; D012754:Sharks
D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D009602:Nitrosamines; D011509:Proteoglycans; C013622:nitrosobis(2-oxopropyl)amine