X-ray sources and high-throughput data collection methods.
X-ray diffraction experiments on protein crystals are at the core of the structure determination process. An overview of X-ray sources and data collection methods to support structure-based drug design (SBDD) efforts is presented in this chapter. First, methods of generating and manipulating X-rays for the purpose of protein crystallography, as well as the components of the diffraction experiment setup are discussed. SBDD requires the determination of numerous protein-ligand complex structures in a timely manner, and the second part of this chapter describes how to perform diffraction experiments efficiently on a large number of crystals, including crystal screening and data collection.
Takeda San Diego, San Diego, CA, USA. GSNELL@TAKEDASD.COM
Gyorgy
G
Snell
D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D015195:Drug Design; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D014965:X-Rays
D011506:Proteins