Surface modification of magnetite nanoparticles using gluconic acid and their application in immobilized lipase.
Superparamagnetic magnetite nanoparticles (SMN) were surface-modified with gluconic acid (GLA) to improve their hydrophilicity and bio-affinity. Gluconic acid was successfully coated on the surface of magnetite nanoparticles and characterized using Fourier transform infrared spectroscopy (FT-IR). With water-soluble carbodiimide (EDC) as the coupling reagent, lipase was successfully immobilized onto the hydroxyl-functionalized magnetic nanoparticles. The immobilized lipase had better resistance to temperature and pH inactivation in comparison to the free form and hence widened the reaction pH and temperature range. Thermostability and storage stability of the enzyme improved upon covalent immobilization. Immobilized lipase showed higher activity after recycling when compared to the free one and could be recovered by magnetic separation.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, PR China.
Ying
Y
Sui
Yu
Y
Cui
Yong
Y
Nie
Guang-Ming
GM
Xia
Guo-Xin
GX
Sun
Jing-Tian
JT
Han
D002175:Candida; D002234:Carbodiimides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017743:Equipment Reuse; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005942:Gluconates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008049:Lipase / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017348:Microscopy, Electron, Scanning Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005942:Gluconates; D058185:Magnetite Nanoparticles; D008049:Lipase; C030691:gluconic acid; D052203:Ferrosoferric Oxide