A photochemical approach for controlled drug release in targeted drug delivery.
Photochemistry provides a unique mechanism that enables the active control of drug release in cancer-targeting drug delivery. This study investigates the light-mediated release of methotrexate, an anticancer drug, using a photocleavable linker strategy based on o-nitrobenzyl protection. We evaluated two types of the o-nitrobenzyl-linked methotrexate for the drug release study and further extended the study to a fifth-generation poly(amidoamine) dendrimer carrier covalently conjugated with methotrexate via the o-nitrobenzyl linker. We performed the drug release studies by using a combination of three standard analytical methods that include UV/vis spectrometry, (1)H NMR spectroscopy, and anal. HPLC. This article reports that methotrexate is released by the photochemical mechanism in an actively controlled manner. The rate of the drug release varies in response to multiple control parameters, including linker design, light wavelength, exposure time, and the pH of the medium where the drug release occurs.
Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109, USA. skchoi@umich.edu
Seok Ki
SK
Choi
Manisha
M
Verma
Justin
J
Silpe
Ryan E
RE
Moody
Kenny
K
Tang
Jeffrey J
JJ
Hanson
James R
JR
Baker
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry; D009578:Nitrobenzenes / Q000737:chemistry*; D010782:Photolysis
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D050091:Dendrimers; D009578:Nitrobenzenes; C104700:PAMAM Starburst; C036077:nitrobenzene; D008727:Methotrexate