[Determination of gibberellins in Arabidopsis thaliana by matrix solid-phase dispersion extraction and high performance liquid chromatography-tandem mass spectrometry].
A method for the analysis of gibberellin A1 (GA1), gibberellin A3 (GA3) and gibberellin A4 (GA4) in Arabidopsis thaliana by matrix solid-phase dispersion extraction (MSPD) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The solid sample of Arabidopsis thaliana was gently blended with C18 to obtain a homogeneous mixture. This mixture was transferred to an SPE cartridge filled with 0.5 g C18 to form a MSPD column. GA1, GA3 and GA4 were eluted with cold 80% methanol aqueous solution. The target compounds were separated on a C18 column with a gradient elution of 0.05% formic acid aqueous solution and acetonitrile as the mobile phase. The identification and quantification were carried out by using electrospray ionization in negative ion mode (ESI-) with multiple reaction monitoring (MRM). The linear ranges for GA1, GA3 and GA4 were all from 10 to 300 ng/g with correlation coefficients greater than 0.98. The limits of detection were in the range of 1.1-4.1 ng/g. The average recoveries and relative standard deviations were 54.7%-102.6% and 3.2%-12.8% respectively in the spiked range of 10-50 ng/g. The method is simple, sensitive, efficient and accurate. It is suitable for the confirmation and quantitative determination of GA1, GA3 and GA4 in Arabidopsis thaliana.
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Lu
L
Wang
Qian
Q
Wu
Chunfeng
C
Duan
Dapeng
D
Wu
Yafeng
Y
Guan
D017360:Arabidopsis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005875:Gibberellins / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D005875:Gibberellins