Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy.
Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion, and crossover during cell-cell and cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40.
Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia, Barcelona Science Park, University of Barcelona, Barcelona, Spain.
Sara
S
Nocentini
Diego
D
Reginensi
Simón
S
Garcia
Patricia
P
Carulla
María Teresa
MT
Moreno-Flores
Francisco
F
Wandosell
Xavier
X
Trepat
Ana
A
Bribian
José A
JA
del Río
D000818:Animals; D002465:Cell Movement; D058948:Cell Tracking; D058851:GPI-Linked Proteins / Q000502:physiology; D051379:Mice; D009185:Myelin Proteins / Q000378:metabolism / Q000502:physiology*; D009186:Myelin Sheath / Q000378:metabolism; D000070816:Nogo Receptor 1; D009830:Olfactory Bulb / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000502:physiology
D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat