Ruegeria conchae sp. nov., isolated from the ark clam Scapharca broughtonii.
A slightly halophilic, Gram-negative, strictly aerobic, non-motile rod, designated TW15(T), was isolated from an ark clam in South Korea. Growth occurred at 10-37 °C, with 1-5% (w/v) NaCl and at pH 7.0-10.0. Optimal growth occurred at 25-30 °C, with 2% (w/v) NaCl and at pH 8.0. Strain TW15(T) exhibited both oxidase and catalase activities. The major fatty acids of strain TW15(T) were summed feature 8 (consisting of C(18:1)ω7c and/or C(18:1)ω6c) and 11-methyl C(18:1)ω7c. The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The polar lipids of strain TW15(T) comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and five unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TW15(T) was most closely related to Ruegeria lacuscaerulensis DSM 11314(T) (98.0% 16S rRNA gene sequence similarity). DNA-DNA relatedness with closely related strains was <52 ± 3%. The DNA G+C content was 55.7 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain TW15(T) represents a novel species of the genus Ruegeria, for which the name Ruegeria conchae sp. nov. is proposed. The type strain is TW15(T) ( = KACC 15115(T)  = JCM 17315(T)).
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jina
J
Lee
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Tae Woong
TW
Whon
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Na-Ri
NR
Shin
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Seong Woon
SW
Roh
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jandi
J
Kim
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Seong-Kyu
SK
Park
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Min-Soo
MS
Kim
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Kee-Sun
KS
Shin
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Jung-Sook
JS
Lee
Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Keun Chul
KC
Lee
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, Republic of Korea.
Young-Ok
YO
Kim
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jin-Woo
JW
Bae
D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D049894:Scapharca / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2