Discrete assembly of synthetic peptide-DNA triplex structures from polyvalent melamine-thymine bifacial recognition.
We have designed a 21-residue α-peptide that simultaneously recognizes two decadeoxyoligothymidine (dT(10)) tracts to form triplexes with a peptide-DNA strand ratio of 1:2. The synthetic peptide side chain displays 10 melamine rings, which provide a bifacial thymine-recognition interface along the length of the 21-residue peptide. Recognition is selective for thymine over other nucleobases and drives the formation of ternary peptide·[dT(10)](2) complexes as well as heterodimeric peptide·[dT(10)C(10)T(10)] hairpin structures with triplex stems.
Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
Yingying
Y
Zeng
Yaowalak
Y
Pratumyot
Xijun
X
Piao
Dennis
D
Bong
D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D015394:Molecular Structure; D053758:Nanoparticles; D010455:Peptides / Q000138:chemical synthesis*; D013941:Thymine / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry*
D046911:Macromolecular Substances; D010455:Peptides; D014227:Triazines; C051802:triplex DNA; D004247:DNA; C011907:melamine; D013941:Thymine