The study of single anticancer peptides interacting with HeLa cell membranes by single molecule force spectroscopy.
To determine the effects of biophysical parameters (e.g. charge, hydrophobicity, helicity) of peptides on the mechanism of anticancer activity, we applied a single molecule technique-force spectroscopy based on atomic force microscope (AFM)-to study the interaction force at the single molecule level. The activity of the peptide and analogs against HeLa cells exhibited a strong correlation with the hydrophobicity of peptides. Our results indicated that the action mode between α-helical peptides and cancer cells was largely hydrophobicity-dependent.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Yuping
Y
Shan
Jinfeng
J
Huang
Juanjuan
J
Tan
Gui
G
Gao
Shuheng
S
Liu
Hongda
H
Wang
Yuxin
Y
Chen
D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D017433:Protein Structure, Secondary
D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents