Neurotrophic features of human adipose tissue-derived stromal cells: in vitro and in vivo studies.
Due to its abundance, easy retrieval, and plasticity characteristics, adipose-tissue-derived stromal cells (ATSCs) present unquestionable advantages over other adult-tissue-derived stem cells. Based on the in silico analysis of our previous data reporting the ATSC-specific expression profiles, the present study attempted to clarify and validate at the functional level the expression of the neurospecific genes expressed by ATSC both in vitro and in vivo. This allowed evidencing that ATSCs express neuro-specific trophins, metabolic genes, and neuroprotective molecules. They were in fact able to induce neurite outgrowth in vitro, along with tissue-specific commitment along the neural lineage and the expression of the TRKA neurotrophin receptor in vivo. Our observation adds useful information to recent evidence proposing these cells as a suitable tool for cell-based applications in neuroregenerative medicine.
Institute of Anatomy and Cell Biology, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Wanda
W
Lattanzi
Maria Concetta
MC
Geloso
Nathalie
N
Saulnier
Stefano
S
Giannetti
Maria Ausiliatrice
MA
Puglisi
Valentina
V
Corvino
Antonio
A
Gasbarrini
Fabrizio
F
Michetti
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D017077:Culture Media, Conditioned; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D008875:Middle Aged; D016501:Neurites / Q000378:metabolism*; D016716:PC12 Cells; D061251:Primary Cell Culture; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism*; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; D017077:Culture Media, Conditioned; D017475:Receptors, Nerve Growth Factor; C093649:TRO protein, human