Hypothalamic expression of urocortin 3 and the type 2 corticotropin-releasing factor receptor is regulated according to feeding state in lean but not obese Zucker rats.
Urocortin 3 (Ucn3) is an anorexigenic neuropeptide with high affinity for the type 2 corticotropin-releasing factor receptor (CRF₂-R). How the expression of hypothalamic Ucn3 is regulated by fasting and refeeding in genetically obese (fa/fa) Zucker rats is not known. Obese Zucker rats develop early hyperphagia associated with low expression of CRF₂-R in the ventromedial hypothalamic nucleus (VMH) in this phenotype. Although lean (Fa/?) Zucker rats have strong basal expression of CRF₂-R in the VMH, and normally consume less food compared to their obese littermates, at the beginning of refeeding, the lean rats ingested almost the same amount of food as the obese animals. The present study was designed to investigate the dynamics of the expression of CRF₂-R and Ucn3 in the brain of lean and obese Zucker rats fed ad libitum, food-deprived for 48 h, or refed for 1 and 24 h. The levels of expression of Ucn3 mRNA were analyzed in the rostral perifornical hypothalamus (rPFH) and dorsal medial amygdala (MeD), and CRF₂-R mRNA in the VMH and lateral septum (LS) using in situ hybridization. The results showed that in the ad libitum-fed state, both phenotypes had comparable levels of expression of rPFH Ucn3, but the obese rats had lower levels of expression of VMH CRF₂-R. Food deprivation decreased hypothalamic expression of Ucn3 and CRF₂-R in lean but not obese rats. One hour of refeeding triggered expression of rPFH Ucn3 but not VMH CRF₂-R in lean rats, and at 24 h of refeeding the levels of hypothalamic expression of Ucn3 and CRF₂-R returned to those seen in the ad libitum-fed state in both phenotypes. In the LS, the levels of expression of CRF₂-R were not affected by feeding and phenotype. In the MeD, the Ucn3 transcript increased by food deprivation in obese but not lean rats. Therefore, the increase of Ucn3 expression in the MeD in obese food-deprived rats may reflect stronger behavioral effects of food deprivation in this phenotype. The hypothalamic expression of Ucn3 and CRF₂)-R was modulated by the feeding states in lean but not obese rats. The low levels of VMH CRF₂-R may limit anorexigenic Ucn3 effects in the obese phenotype. The low VMH CRF₂-R levels at the beginning of refeeding in lean rats may allow them to ingest a considerable amount of food regardless of the rapidly increased expression of rPFH Ucn3 by refeeding in this phenotype. This article is part of a Special Issue entitled 'Central Control of Food Intake'.
Faculté de Médecine, Département Psychiatrie et Neurosciences, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec-QC G1V 4G5, Canada.
Anne-Marie
AM
Poulin
Christophe
C
Lenglos
Arojit
A
Mitra
Elena
E
Timofeeva
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D004195:Disease Models, Animal; D004435:Eating / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011924:Rats, Zucker; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D054832:Urocortins / Q000235:genetics / Q000378:metabolism*
C101531:CRF receptor type 2; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins