Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters.
In this study, fluorescent metal nanoclusters are presented as novel probes for sensitive detection of protease for the first time. The sensing mechanism is based on trypsin digestion of the protein template of BSA-stabilized Au nanoclusters. The decrease in fluorescence intensity of BSA-Au nanoclusters caused by trypsin allows the sensitive detection of trypsin in the range of 0.01-100 μg/mL. The detection limit for trypsin is 2 ng/mL (86 pM) at a signal-to-noise ratio of 3. The present nanosensor for trypsin detection possesses red emission, excellent biocompatibility, high selectivity, and good stability. In addition, we demonstrated the application of the present approach in real urine samples, which suggested its potential for diagnostic purposes.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Lianzhe
L
Hu
Shuang
S
Han
Saima
S
Parveen
Yali
Y
Yuan
Ling
L
Zhang
Guobao
G
Xu
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D059629:Signal-To-Noise Ratio; D014357:Trypsin / Q000032:analysis* / Q000378:metabolism / Q000652:urine*
D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006046:Gold; D014357:Trypsin