Electrophysiological methods for Caenorhabditis elegans neurobiology.
Patch-clamp electrophysiology is a technique of choice for the biophysical analysis of the function of nerve, muscle, and synapse in Caenorhabditis elegans nematodes. Considerable technical progress has been made in C. elegans electrophysiology in the decade since the initial publication of this technique. Today, most, if not all, electrophysiological studies that can be done in larger animal preparations can also be done in C. elegans. This chapter has two main goals. The first is to present to a broad audience the many techniques available for patch-clamp analysis of neurons, muscles, and synapses in C. elegans. The second is to provide a methodological introduction to the techniques for patch clamping C. elegans neurons and body-wall muscles in vivo, including emerging methods for optogenetic stimulation coupled with postsynaptic recording. We also present samples of the cell-intrinsic and postsynaptic ionic currents that can be measured in C. elegans nerves and muscles.
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, USA.
Miriam B
MB
Goodman
Theodore H
TH
Lindsay
Shawn R
SR
Lockery
Janet E
JE
Richmond
D000200:Action Potentials / Q000528:radiation effects; D000269:Adhesives; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000502:physiology* / Q000528:radiation effects; D004566:Electrodes; D007103:Immobilization; D008027:Light; D042282:Microdissection; D009132:Muscles / Q000502:physiology* / Q000528:radiation effects; D009446:Neurobiology / Q000379:methods*; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods*; D010775:Photic Stimulation; D013569:Synapses / Q000502:physiology* / Q000528:radiation effects; D055366:Synaptic Potentials / Q000528:radiation effects
D000269:Adhesives