Counting Legionella cells within single amoeba host cells.
Here we present the first attempt to quantify Legionella pneumophila cell numbers within individual amoeba hosts that may be released into engineered water systems. The maximum numbers of culturable L. pneumophila cells grown within Acanthamoeba polyphaga and Naegleria fowleri were 1,348 (mean, 329) and 385 (mean, 44) CFU trophozoite(-1), respectively.
National Exposure Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Cincinnati, Ohio, USA. buse.helen@epa.gov
Helen Y
HY
Buse
Nicholas J
NJ
Ashbolt
D000048:Acanthamoeba / Q000382:microbiology*; D058491:Bacterial Load; D016952:Legionella pneumophila / Q000302:isolation & purification*; D016848:Naegleria fowleri / Q000382:microbiology*