Quantitative proteomics reveals dynamic changes in the plasma membrane during Arabidopsis immune signaling.
The plant plasma membrane is a crucial mediator of the interaction between plants and microbes. Understanding how the plasma membrane proteome responds to diverse immune signaling events will lead to a greater understanding of plant immunity and uncover novel targets for crop improvement. Here we report the results from a large scale quantitative proteomics study of plasma membrane-enriched fractions upon activation of the Arabidopsis thaliana immune receptor RPS2. More than 2300 proteins were identified in total, with 1353 proteins reproducibly identified across multiple replications. Label-free spectral counting was employed to quantify the relative protein abundance between different treatment samples. Over 20% of up-regulated proteins have known roles in plant immune responses. Significantly changing proteins include those involved in calcium and lipid signaling, membrane transport, primary and secondary metabolism, protein phosphorylation, redox homeostasis, and vesicle trafficking. A subset of differentially regulated proteins was independently validated during bacterial infection. This study presents the largest quantitative proteomics data set of plant immunity to date and provides a framework for understanding global plasma membrane proteome dynamics during plant immune responses.
University of California at Davis, Davis, California 95616, USA.
James Mitch
JM
Elmore
Jun
J
Liu
Barrett
B
Smith
Brett
B
Phinney
Gitta
G
Coaker
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology*; D002853:Chromatography, Liquid; D003907:Dexamethasone / Q000494:pharmacology; D030821:Plants, Genetically Modified / Q000276:immunology / Q000382:microbiology; D040901:Proteomics / Q000379:methods*; D011552:Pseudomonas Infections / Q000276:immunology; D053719:Tandem Mass Spectrometry
D029681:Arabidopsis Proteins; C089426:RPS2 protein, Arabidopsis; D003907:Dexamethasone