Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold Aspergillus oryzae.
A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Akihiro
A
Sugita
Ayako
A
Sugii
Kazuaki
K
Sato
Xiao-Yong
XY
Zhang
An-Lan
AL
Dai
Goro
G
Taguchi
Makoto
M
Shimosaka
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D005110:Extracellular Space / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D006026:Glycoside Hydrolases; C019868:chitosanase