An electrochemiluminescent assay for high sensitive detection of mercury (II) based on isothermal rolling circular amplification.
In this study, we firstly demonstrated that Bst DNA polymerase shows specific recognition and function on the T-Hg(2+)-T biomimetic structure. Based on this, a novel available electrochemiluminescence (ECL) sensor for Hg(2+) has been developed. In this strategy, magnet beads tagged primer was designed to complementary to the region of the circular padlock probe but with two T-T mismatches at the 3' end. The mismatched primers cannot be extended by Bst DNA polymerase in the absence of Hg(2+). Stable T-Hg(2+)-T can be formed in the presence of Hg(2+), thus induces the elongation and amplification reaction by DNA polymerase with a rolling circular amplification (RCA) mechanism. Subsequently, the resulted RCA products are hybridized with the tris (bipyridine) ruthenium (TBR)-tagged probes and detected by ECL platform. Current method shows a sub-nanomolar sensitivity and excellent selectivity over a spectrum of interference metal ions.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Xiaoming
X
Zhou
Qiang
Q
Su
Da
D
Xing
D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D007477:Ions / Q000737:chemistry; D008163:Luminescent Measurements; D008628:Mercury / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques
D015342:DNA Probes; D007477:Ions; D004259:DNA-Directed DNA Polymerase; D008628:Mercury