The protective effect of adrenomedullin on renal injury, in a model of abdominal aorta cross-clamping.
Renal injury induced by aortic ischemia-reperfusion (IR) is an important factor in the development of postoperative acute renal failure following abdominal aortic surgery. The aim of this study was to examine the effect of adrenomedullin (AM) on kidney injury induced by infrarenal abdominal aortic IR in rats. Thirty-two Wistar Albino rats were randomized into four groups (eight per group) as follows: Control group, IR group (120-minute ischemia and 120-minute reperfusion), IR + AM group (a bolus intravenously of 0.05 µg/kg/min AM), and control + AM group. At the end of the experiment, blood and kidney tissue specimens were obtained for biochemical analysis. Immunohistological evaluation of the rat kidney tissues was also done. IR significantly increased (p < 0.05 vs control group) and AM significantly decreased (p < 0.05 vs. IR group) all of the biochemical parameters. Immunohistological evaluation showed that AM attenuated morphological changes as apoptosis associated with kidney injury. The results of this study indicate that AM attenuates both biochemically and immunohistopathologically kidney injury induced by aortic IR in rats.
Department of Physiology, Faculty of Medicine, Gazi University, Ankara, Turkey. eserozoyar@yahoo.com
Eser Öz
EÖ
Oyar
Ilker
I
Kiriş
Senol
S
Gülmen
Betül Mermi
BM
Ceyhan
Medine Cumhur
MC
Cüre
Namık
N
Delibaş
Neşe
N
Lortlar
Hüseyin
H
Okutan
D058186:Acute Kidney Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D053607:Adrenomedullin / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001012:Aorta, Abdominal / Q000601:surgery*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D003250:Constriction; D019610:Cytoprotection; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000097:blood; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014656:Vascular Surgical Procedures / Q000009:adverse effects*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D018836:Inflammation Mediators; D053607:Adrenomedullin