Developmental validation of RSID™-Semen: a lateral flow immunochromatographic strip test for the forensic detection of human semen.
Tests for the identification of semen commonly involve the microscopic visualization of spermatozoa or assays for the presence of seminal markers such as acid phosphatase (AP) or prostate-specific antigen (PSA). Here, we describe the rapid stain identification kit for the identification of semen (RSID™-Semen), a lateral flow immunochromatographic strip test that uses two antihuman semenogelin monoclonal antibodies to detect the presence of semenogelin. The RSID™-Semen strip is specific for human semen, detecting <2.5 nL of semen, and does not cross-react with other human or nonhuman tissues tested. RSID™-Semen is more sensitive with certain forensic evidence samples containing mixtures of vaginal secretions and semen than either of the commercially available PSA-based forensic semen detection tests or tests that measure AP activity that were tested in parallel. The RSID™-Semen kit also allows sampling a fraction of a questioned stain while retaining the majority of the sample for further processing through short tandem repeat analysis.
Independent Forensics of Illinois, 4600 Roosevelt Road, Hillside, IL 60162, USA.
Jennifer
J
Old
Brett A
BA
Schweers
Pravat W
PW
Boonlayangoor
Brian
B
Fischer
Kevin W P
KW
Miller
Karl
K
Reich
D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods*; D016172:DNA Fingerprinting; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011934:Reagent Strips; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D029607:Seminal Vesicle Secretory Proteins / Q000276:immunology / Q000302:isolation & purification; D013045:Species Specificity; D013048:Specimen Handling
D000911:Antibodies, Monoclonal; D011934:Reagent Strips; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen