Effects of lactic acid-fermented soymilk on lipid metabolism-related gene expression in rat liver.
We examined the effects of lactic acid fermentation of soymilk on the lipid profile and lipid metabolism-related gene expression in rat liver. Male Sprague-Dawley rats aged 7 weeks were fed a control diet (AIN-93), soymilk diet, or fermented soymilk diet for 1 week or 5 weeks. The hepatic triglyceride and cholesterol contents in the soymilk (SM) group and the fermented soymilk (FSM) group were significantly lower than those in the control group after 5 weeks, but these changes had not become apparent until after 1 week. The fatty acid synthesis-related genes were more markedly down-regulated after 1 week than after 5 weeks, whereas the cytochrome p450 family 7 subfamily a polypeptide 1 (CYP7al) gene related to cholesterol catabolism was more markedly up-regulated after 5 weeks than after 1 week. This up-regulation was higher in the FSM group than in the SM group. It is assumed that the bioactive components produced by lactic acid fermentation induced the up-regulation of CYP7a1.
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan. maki1002@mukogawa-u.ac.jp
Maki
M
Kobayashi
Tomoko
T
Harada
Naohiro
N
Takagi
Kimiko
K
Tsuzuki
Makoto
M
Sugawara
Mitsuru
M
Fukuda
D000818:Animals; D001835:Body Weight; D004032:Diet; D004435:Eating; D005285:Fermentation; D005786:Gene Expression Regulation / Q000187:drug effects*; D007529:Isoflavones / Q000032:analysis / Q000097:blood; D019344:Lactic Acid / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D041942:Soy Milk / Q000378:metabolism* / Q000494:pharmacology*; D013997:Time Factors
D007529:Isoflavones; D008055:Lipids; D019344:Lactic Acid