A novel evaluation method for extrapolated retention factor in determination of n-octanol/water partition coefficient of halogenated organic pollutants by reversed-phase high performance liquid chromatography.
The retention factor corresponding to pure water in reversed-phase high performance liquid chromatography (RP-HPLC), k(w), was commonly obtained by extrapolation of retention factor (k) in a mixture of organic modifier and water as mobile phase in tedious experiments. In this paper, a relationship between logk(w) and logk for directly determining k(w) has been proposed for the first time. With a satisfactory validation, the approach was confirmed to enable easy and accurate evaluation of k(w) for compounds in question with similar structure to model compounds. Eight PCB congeners with different degree of chlorination were selected as a training set for modeling the logk(w)-logk correlation on both silica-based C(8) and C(18) stationary phases to evaluate logk(w) of sample compounds including seven PCB, six PBB and eight PBDE congeners. These eight model PCBs were subsequently combined with seven structure-similar benzene derivatives possessing reliable experimental K(ow) values as a whole training set for logK(ow)-logk(w) regressions on the two stationary phases. Consequently, the evaluated logk(w) values of sample compounds were used to determine their logK(ow) by the derived logK(ow)-logk(w) models. The logK(ow) values obtained by these evaluated logk(w) were well comparable with those obtained by experimental-extrapolated logk(w), demonstrating that the proposed method for logk(w) evaluation in this present study could be an effective means in lipophilicity study of environmental contaminants with numerous congeners. As a result, logK(ow) data of many PCBs, PBBs and PBDEs could be offered. These contaminants are considered to widely exist in the environment, but there have been no reliable experimental K(ow) data available yet.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
Shu-ying
SY
Han
Chao
C
Liang
Jun-qin
JQ
Qiao
Hong-zhen
HZ
Lian
Xin
X
Ge
Hong-yuan
HY
Chen
D020003:1-Octanol / Q000737:chemistry*; D056148:Chromatography, Reverse-Phase; D004785:Environmental Pollutants / Q000032:analysis* / Q000302:isolation & purification; D055768:Halogenated Diphenyl Ethers / Q000032:analysis / Q000302:isolation & purification; D054879:Halogenation; D011075:Polybrominated Biphenyls / Q000032:analysis / Q000302:isolation & purification; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000302:isolation & purification; D014867:Water / Q000737:chemistry*
D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; D014867:Water; D011078:Polychlorinated Biphenyls; D020003:1-Octanol