Hsp70 promotes synaptic transmission in brain slices damaged by contact with blood clot.
Neurotrophic and neuroprotective properties of Hsp70 were studied in olfactory cortex slices of hypertensive rats under normal conditions and under influence of autologous blood (blood clot). Under normal conditions, synaptic response in slices depended on Hsp70 amount in medium. Incubation of slices with Hsp70 showed a biphasic effect on electrogenesis in tissue. Hsp70 (from 0.1 to 3.0 μg/ml) stimulated glutamatergic and GABA-ergic receptor activity. An inhibitory action on synaptic response is detected at increased amounts of Hsp70 (>4 μg/ml). Pretreatment with Hsp70 (1 and 10 μg/ml) prevented the irreversible inhibition of bioelectric activity in neurons damaged by autologous blood action and interfered with swelling in slices. Our results indicate that exogenous Hsp70 may play a role in the maintenance of neuronal synaptic functions in normal conditions, and protects electrogenesis of brain tissue in strokes.
Lab. Regulation of Neuron Function, I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences, 199034, nab. Makarova 6, St. Petersburg, Russia.
Anatoly A
AA
Mokrushin
Larisa I
LI
Pavlinova
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001921:Brain / Q000166:cytology* / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D055724:Electrophysiological Phenomena / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D006973:Hypertension / Q000473:pathology / Q000503:physiopathology; D066298:In Vitro Techniques; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D005680:gamma-Aminobutyric Acid