Comparison of chromogenic urinary tract infection medium with cysteine lactose electrolyte deficient media in a resource limited setting.
OBJECTIVE
To compare the chromogenic UTI medium (CUM) with cysteine lactose electrolyte deficient medium (CLED) in terms of isolation of uropathogens, turnaround time and cost.


METHODS
A total of 251 urine samples were selected and inoculated on both CLED and CUM, growth was observed after 24 and 48 hours of incubation. Isolates were identified by colony's colour and biochemical tests. Turnaround time for identification and cost was calculated till final identification of microorganisms.


RESULTS
A discrepancy in isolation was observed in seven samples with growth on CUM in 24 hours while in 48 hours on CLED. There was 100% agreement in identification by both media. Almost 50% samples were identified within 24 hours by using CUM in contrast to CLED where most samples were identified in 48 hours. Total number of reagents used and total cost for processing of a specimen including technologist and consultant time by using CUM is significantly low in comparison to CLED.


CONCLUSIONS
CUM can replace CLED as a primary isolation media for urine culture in clinical laboratories in Pakistan as it is user friendly, facilitates early reporting and saves cost.
Department of Microbiology, Aga Khan University Hospital.
Saba
S
Qaiser
Muhammad
M
Zeeshan
Kausar
K
Jabeen
Tanwir
T
Ahsan
Afia
A
Zafar
D001419:Bacteria / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D002863:Chromogenic Compounds / Q000737:chemistry / Q000191:economics; D015169:Colony Count, Microbial; D003365:Costs and Cost Analysis; D003470:Culture Media / Q000737:chemistry* / Q000191:economics*; D003545:Cysteine; D006784:Hospitals, Teaching; D006801:Humans; D007785:Lactose; D010154:Pakistan; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology; D014556:Urine / Q000382:microbiology*
D002863:Chromogenic Compounds; D003470:Culture Media; D007785:Lactose; D003545:Cysteine