Effects of Sideritis euboea (Lamiaceae) aqueous extract on IL-6, OPG and RANKL secretion by osteoblasts.
The water extract obtained from the aerial parts of Sideritis euboea (Lamiaceae), which is known in Greece as 'mountain tea', was investigated by determining the in vitro effect of this extract on the IL-6, OPG and RANKL secretion by osteoblasts, three important molecules in osteoblast-osteoclast interplay. The results showed that this extract reduced significantly the secretion of IL-6 by KS-483 osteoblasts, while it also suppressed RANKL secretion, with both effects being dose-dependent and more potent at the higher concentrations tested (50, 100 microg/mL). We further determined the chemical profile of the extract by applying an analytical U-HPLC-DAD-ESI-MS/MS method using the high resolution hybrid LTQ-Orbitrap Discovery spectrometer. An ESI source in negative mode was employed. The analysis indicated that the water extract of S. euboea is rich in flavonoid glycosides, and phenylpropanoid glycosides.
Department of Biological Chemistry, Medical School, University of Athens, 75 M Asias, Goudi, 11527 Athens, Greece.
Eva
E
Kassi
Anna
A
Paliogianni
Ismene
I
Dontas
Nektarios
N
Aligiannis
Maria
M
Halabalaki
Zoi
Z
Papoutsi
Alexios-Leandros
AL
Skaltsounis
Paraskevi
P
Moutsatsou
D000818:Animals; D002460:Cell Line; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D053244:Osteoprotegerin / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism; D031365:Sideritis / Q000737:chemistry*
D015850:Interleukin-6; D053244:Osteoprotegerin; D010936:Plant Extracts; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse