Expression and possible role of interleukin-10 receptors in patients with adenomyosis.
OBJECTIVE
To investigate the expression and potential roles of interleukin-10 receptor 1 (IL-10R1) and interleukin-10 receptor 2 (IL-10R2) in adenomyosis.


METHODS
This prospective study examined 33 women with histologically proven adenomyosis and 21 women without adenomyosis who had undergone hysterectomy for non-endometrial pathology. Comparative immunohistochemistry was used to evaluate the expression and localization of IL-10R1 and IL-10R2. Tissue sections were immunostained with goat anti-human interleukin-10 receptor alpha and rabbit anti-human interleukin-10 receptor beta antibodies. The presence and localization of IL-10R1 and IL-10R2 were evaluated microscopically throughout the menstrual cycle in eutopic and ectopic endometrial tissues of women with adenomyosis, and the results were compared with those for normal endometrium.


RESULTS
IL-10R1 and IL-10R2 were mainly expressed by epithelial cells in both women with adenomyosis and controls. Epithelial expression of IL-10R1 and IL-10R2 was higher in adenomyotic samples than in eutopic endometrium of women with adenomyosis or normal endometrium. Moreover, epithelial expression of IL-10R1 was higher in eutopic endometrium of women with adenomyosis than in normal endometrium. Epithelial expression of IL-10R1 showed cyclic variation in eutopic endometrium of women with adenomyosis and normal endometrium, with elevated expression in secretory-phase tissues compared with proliferative-phase tissues.


CONCLUSIONS
Intrinsic abnormalities concerning IL-10 and IL-10 receptors may be present in eutopic and ectopic endometria of women with adenomyosis. These findings suggest that IL-10 receptors may be involved in the immunotolerant and/or anti-inflammatory process of adenomyosis.
Department of Obstetrics and Gynaecology, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, People's Republic of China.
Xiaoyan
X
Qin
Hualing
H
Zhang
Fei
F
Wang
Jing
J
Xue
Zeqing
Z
Wen
D000328:Adult; D000704:Analysis of Variance; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D053709:Interleukin-10 Receptor alpha Subunit / Q000378:metabolism*; D053710:Interleukin-10 Receptor beta Subunit / Q000378:metabolism*; D008597:Menstrual Cycle / Q000378:metabolism; D008875:Middle Aged; D011446:Prospective Studies; D014591:Uterine Diseases / Q000378:metabolism*
D053709:Interleukin-10 Receptor alpha Subunit; D053710:Interleukin-10 Receptor beta Subunit