Cellular and molecular damage of Phanerochaete chrysosporium by the oxidation hair dyes.
BACKGROUND
The toxic effect of the oxidation hair dyes on Phanerochaete chrysosporium was investigated by exposure of this fungus in a nitrogen-limited culture medium to various concentrations of the oxidation hair dyes.


RESULTS
The results showed that both the size and the dry weight of the mycelial pellets of P. chrysosporium could be reduced when the concentration of the oxidation hair dyes was higher than 300 mg/L. By using the AFLP analysis and the UPGMA dendrogram, the DNA damage of P. chrysosporium by the oxidation hair dyes was also detected. Comparing with that in the control, the percent polymorphism under different concentrations of the oxidation hair dyes increased. In the meantime, the DNA similarity was decreased, which meant that the DNA damage was aggravated with an increase in the concentrations of the oxidation hair dyes.


CONCLUSIONS
Thus, as an environmental pollutant, the oxidation hair dyes have a toxic effect on P. chrysosporium at both cellular and molecular levels.
Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, 94 Weijin Road, Tianjin 300071, People's Republic of China.
Hongxia
H
Xiong
Qixing
Q
Zhou
D054458:Amplified Fragment Length Polymorphism Analysis; D003470:Culture Media; D004249:DNA Damage / Q000187:drug effects; D004271:DNA, Fungal / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity; D006202:Hair Dyes / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D020075:Phanerochaete / Q000187:drug effects*
D003470:Culture Media; D004271:DNA, Fungal; D004785:Environmental Pollutants; D006202:Hair Dyes