Phagocyte-specific S100 proteins in the local response to the Echinococcus granulosus larva.
Infection by larval Echinococcus granulosus is usually characterized by tight inflammatory control. However, various degrees of chronic granulomatous inflammation are also observed, reaching a high point in infection of cattle by the most prevalent parasite strain worldwide, which is not well adapted to this host species. In this context, epithelioid and multinucleated giant macrophages surround the parasite, and the secreted products of these cells often associate with the larval wall. The phagocyte-specific S100 proteins, S100A8, S100A9 and S100A12, are important non-conventionally secreted amplifiers of inflammatory responses. We have analysed by proteomics and immunohistochemistry the presence of these proteins at the E. granulosus larva-host interface. We found that, in the context of inflammatory control as observed in human infections, the S100 proteins are not abundant, but S100A9 and S100A8 can be expressed by eosinophils distal to the parasite. In the granulomatous inflammation context as observed in cattle infections, we found that S100A12 is one of the most abundant host-derived, parasite-associated proteins, while S100A9 and S100A8 are not present at similarly high levels. As expected, S100A12 derives mostly from the epithelioid and multinucleated giant cells. S100A12, as well as cathepsin K and matrix metalloproteinase-9, also expressed by E. granulosus-elicited epithelioid cells, are connected to the Th17 arm of immunity, which may therefore be involved in this granulomatous response.
Cátedra de Inmunología, Departamento de Biociencias (Facultad de Química) e Instituto de Química Biológica (Facultad de Ciencias), Universidad de la República, Montevideo, Uruguay.
Tatiana
T
Basika
Natalia
N
Muñoz
Cecilia
C
Casaravilla
Florencia
F
Irigoín
Carlos
C
Batthyány
Mariana
M
Bonilla
Gustavo
G
Salinas
José Pedro
JP
Pacheco
Johaness
J
Roth
Rosario
R
Durán
Alvaro
A
Díaz
D000818:Animals; D002417:Cattle; D004443:Echinococcosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000502:physiology*; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D007814:Larva / Q000502:physiology; D051379:Mice; D010586:Phagocytes / Q000378:metabolism*; D040901:Proteomics; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D009418:S100 Proteins