Complete genome sequence of the podoviral bacteriophage ΦCP24R, which is virulent for Clostridium perfringens.
Bacteriophage ΦCP24R was isolated from raw sewage from a waste treatment plant, and lytic activity was observed against a type A Clostridium perfringens isolate. Electron microscopy revealed a small virion (44-nm-diameter icosahedral capsid) with a short, non-contractile tail, indicative of a member of the family Podoviridae. The phage had a linear, double-stranded DNA genome of 18,919 base pairs (bp) with 41 bp inverted terminal repeats and a type B DNA polymerase, which are characteristics of members of the subfamily Picovirinae. Out of 22 predicted genes in the genome, ten had significant sequence similarity to proteins of known function. Three distinct genes with lytic domains were identified, including a zinc carboxypeptidase domain that has not been previously reported in viruses. The ΦCP24R genome described herein is only the second Clostridium perfringens podovirus genome reported to date.
Poultry Microbiological Safety Research Unit, Richard B. Russell Agricultural Research Center, Agricultural Research Service, USDA, 950 College Station Road, Athens, GA, 30605, USA. Cesar.Morales@ars.usda.gov
Cesar A
CA
Morales
Brian B
BB
Oakley
Johnna K
JK
Garrish
Gregory R
GR
Siragusa
Mary B
MB
Ard
Bruce S
BS
Seal
D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D003016:Clostridium perfringens / Q000821:virology*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017902:Podoviridae / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012722:Sewage / Q000821:virology; D020079:Terminal Repeat Sequences; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D012722:Sewage; D004247:DNA