Construction of a genetic linkage map based on amplified fragment length polymorphism markers and development of sequence-tagged site markers for marker-assisted selection of the sporeless trait in the oyster mushroom (Pleurotus eryngii).
A large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, including Pleurotus eryngii (DC.) Quél. A cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. In this study, using a sporeless P. eryngii strain, we constructed a genetic linkage map to introduce a molecular breeding program like marker-assisted selection. Based on the segregation of 294 amplified fragment length polymorphism markers, two mating type factors, and the sporeless trait, the linkage map consisted of 11 linkage groups with a total length of 837.2 centimorgans (cM). The gene region responsible for the sporeless trait was located in linkage group IX with 32 amplified fragment length polymorphism markers and the B mating type factor. We also identified eight markers closely linked (within 1.2 cM) to the sporeless locus using bulked-segregant analysis-based amplified fragment length polymorphism. One such amplified fragment length polymorphism marker was converted into two sequence-tagged site markers, SD488-I and SD488-II. Using 14 wild isolates, sequence-tagged site analysis indicated the potential usefulness of the combination of two sequence-tagged site markers in cross-breeding of the sporeless strain. It also suggested that a map constructed for P. eryngii has adequate accuracy for marker-assisted selection.
Tottori University, Tottori, Japan. yasujin6@hotmail.com
Yasuhito
Y
Okuda
Jun
J
Ueda
Yasushi
Y
Obatake
Shigeyuki
S
Murakami
Yukitaka
Y
Fukumasa
Teruyuki
T
Matsumoto
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D005800:Genes, Fungal; D008040:Genetic Linkage; D005819:Genetic Markers; D020076:Pleurotus / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D013172:Spores, Fungal / Q000235:genetics
D004271:DNA, Fungal; D005819:Genetic Markers