Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope.
In order to clarify the structure and the peptide-presentation characteristics of the equine major histocompatibility complex (MHC) class I molecule, a complex of equine MHC class I molecule (ELA-A1 haplotype, 7-6 allele) with mouse β(2)-microglobulin and the cytotoxic T lymphocyte (CTL) epitope Env-RW12 (RVEDVTNTAEYW) derived from equine infectious anaemia virus (EIAV) envelope protein (residues 195-206) was refolded and crystallized. The crystal, which belonged to space group P2(1), diffracted to 2.3 Å resolution and had unit-cell parameters a = 82.5, b = 71.4, c = 99.8 Å, β = 102.9°. The crystal structure contained two molecules in the asymmetric unit. These results should help to determine the first equine MHC class I molecule structure presenting an EIAV CTL epitope.
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Yuanmingyuan Xi Lu No. 2, Haidian District, Beijing 100094, People's Republic of China.
Shugang
S
Yao
Jianxun
J
Qi
Jun
J
Liu
Rong
R
Chen
Xiaocheng
X
Pan
Xiaoying
X
Li
Feng
F
Gao
Chun
C
Xia
D000595:Amino Acid Sequence; D000818:Animals; D018360:Crystallography, X-Ray; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D015686:Gene Products, env / Q000737:chemistry* / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006736:Horses / Q000276:immunology* / Q000378:metabolism; D004860:Infectious Anemia Virus, Equine / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D017510:Protein Folding; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D018984:Epitopes, T-Lymphocyte; D015686:Gene Products, env; D015395:Histocompatibility Antigens Class I