Controlled release of drugs from gradient hydrogels for high-throughput analysis of cell-drug interactions.
In this paper, we report a method to fabricate microengineered hydrogels that contain a concentration gradient of a drug for high-throughput analysis of cell-drug interactions. A microfluidic gradient generator was used to create a concentration gradient of okadaic acid (OA) as a model drug within poly(ethylene glycol) diacrylate hydrogels. These hydrogels were then incubated with MC3T3-E1 cell seeded glass slides to investigate the cell viability through the spatially controlled release of OA. The drug was released from the hydrogel in a gradient manner and induced a gradient of the cell viability. The drug concentration gradient containing hydrogels developed in this study have the potential to be used for drug discovery and diagnostics applications due to their ability to simultaneously test the effects of different concentrations of various chemicals.
World Premier International-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai 980-8577, Japan.
Serge
S
Ostrovidov
Nasim
N
Annabi
Azadeh
A
Seidi
Murugan
M
Ramalingam
Fariba
F
Dehghani
Hirokazu
H
Kaji
Ali
A
Khademhosseini
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D057166:High-Throughput Screening Assays; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D046210:Microfluidic Analytical Techniques; D019319:Okadaic Acid / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry
D020100:Hydrogels; D019319:Okadaic Acid; D011092:Polyethylene Glycols