A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.
Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.
Department of Veterinary Medicine, The University of Cambridge, Cambridge, United Kingdom.
Gordon M
GM
Daly
Nick
N
Bexfield
Judith
J
Heaney
Sam
S
Stubbs
Antonia P
AP
Mayer
Anne
A
Palser
Paul
P
Kellam
Nizar
N
Drou
Mario
M
Caccamo
Laurence
L
Tiley
Graeme J M
GJ
Alexander
William
W
Bernal
Jonathan L
JL
Heeney
D000818:Animals; D001706:Biopsy; D004285:Dogs; D006801:Humans; D008099:Liver / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification*
D012367:RNA, Viral