Q36R polymorphism of KiSS-1 gene in Brazilian head and neck cancer patients.
The KiSS-1 metastasis-suppressor gene (KiSS-1) product (metastin, kisspeptin) is reported to act after binding with the natural ligand of a G-protein coupled receptor and this gene product inhibits chemotaxis, invasion, and metastasis of cells. The aim of this study was to evaluate the Q36R polymorphism of KiSS-1 in patients with head and neck cancer and to compare the results with healthy individuals and its association with clinicopathological parameters. Gender, age, smoking and alcohol consumption were analyzed for 744 individual (252 head and neck cancer patients and in 522 control individuals). The molecular analysis of these individuals was made after extraction of genomic DNA using the SSCP-PCR technique. This study did not reveal any significant differences in genotype frequencies between healthy individuals and patients with head and neck cancer or with the clinical parameters. This study showed an increase frequency of the Q36R polymorphism in pharyngeal cancer.
Genetics and Molecular Biology Research Unit (UPGEM), Faculdade de Medicina de São José do Rio Preto-FAMERP, São José do Rio Preto, SP, CEP 15090-000, Brazil.
Mariângela Torreglosa
MT
Ruiz
Ana Lívia Silva
AL
Galbiatti
Erika Cristina
EC
Pavarino
José Victor
JV
Maniglia
Eny Maria
EM
Goloni-Bertollo
D000428:Alcohol Drinking / Q000009:adverse effects / Q000235:genetics; D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics*; D001938:Brazil; D016022:Case-Control Studies; D003710:Demography; D005260:Female; D020022:Genetic Predisposition to Disease; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D059648:Kisspeptins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000235:genetics
C106628:KISS1 protein, human; D059648:Kisspeptins