Application of fast liquid chromatography for antioxidants analysis.
BACKGROUND
An intensive development of the Fast Liquid Chromatography (FLC) has been recently observed. It makes possible to reduce time analysis and improve resolution as well as sensitivity. The aim of this study was to separate the chosen antioxidants optimization using the FLC method.


METHODS
The three various procedures for antioxidants analysis were compared. Mobile phases containing aqueous solution of formic acid, acetic acid, acetonitrile, and methanol were tested. Limit of detection (LOD), limit of quantification (LOQ), linearity and repeatability of each procedures were determined.


RESULTS
Developed procedure enabled to separate all analytes and allowed to get low LOD levels and good repeatability. This procedure was used for antioxidants analysis in buckwheat and buckwheat products.


CONCLUSIONS
Fast Liquid Chromatography allows to reduce time analysis and obtain good validation parameters.
Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poland. agadro@up.poznan.pl
Agnieszka
A
Drożdżyńska
Krzysztof
K
Dzidzic
Alicja
A
Kośmider
Katarzyna
K
Leja
Katarzyna
K
Czaczyk
Danuta
D
Górecka
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols