A cell-based protein-protein interaction method using a permuted luciferase reporter.
We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another protein (B) fused with the TEV protease. We demonstrate assay applicability for ligand-induced protein-protein interactions including G-protein coupled receptors, receptor tyrosine kinases and nuclear hormone receptors.
BioInvenu Corporation, USA, East Hanover, NJ.
Haifeng
H
Eishingdrelo
Jidong
J
Cai
Paul
P
Weissensee
Praveen
P
Sharma
Michael J
MJ
Tocci
Paul S
PS
Wright