Two types of defective RNAs arising from the tomato black ring virus genome.
Short defective RNAs (D-RNAs) associated with tomato black ring virus (TBRV) were isolated, cloned and sequenced. As a result, two types of D-RNAs associated with different TBRV isolates were identified. Both types were derived from RNA1. The first one contained sequences from the 5' and 3' untranslated regions (UTR) and from the 5' region of a single large open reading frame. The second one included a portion of the coding region for the RNA-dependent RNA polymerase flanked by a short fragment of the 5' UTR and the entire 3' UTR. The possible nature and origin of these RNA species is discussed.
Institute of Plant Protection-National Research Institute, ul. Wł. Węgorka 20, 60-318 Poznań, Poland. B.Hasiow@iorpib.poznan.pl
Beata
B
Hasiów-Jaroszewska
Natasza
N
Borodynko
Marek
M
Figlerowicz
Henryk
H
Pospieszny
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001483:Base Sequence; D003001:Cloning, Molecular; D003673:Defective Viruses / Q000235:genetics* / Q000302:isolation & purification*; D018551:Solanum lycopersicum; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics* / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins