Peroxiredoxin I regulates the component expression of γ-secretase complex causing the Alzheimer's disease.
Peroxiredoxin I (Prx I) is a member of the peroxiredoxins (Prxs) family, which are antioxidant enzymes that regulate various cellular process via intracellular oxidative signal pathways. In order to investigate the correlation between Prx I and the γ-secretase complex, which causes Alzheimer's disease (AD), the expression level of Prx I was firstly evaluated in an animal model for AD. NSE/hPen-2 transgenic (Tg) mice, which were used as animal model in this study, showed a high level of Pen-2 expression and accumulation of Aβ-42 peptides in the hippocampus of brain. The expression level of Prx I was significantly higher on the mRNA and protein level in the brain of this model, while not change in Prx VI expression was observed. Furthermore, to verify the effect of Prx I on the γ-secretase components in vitro, the expression level of these components was analyzed in the Prx I transfectants. Of the components of the γ-secretase complex, the expression of PS-2 and Pen-2 was lower in the transfectants overexpressing Prx I compared to the vector transfectants. However, the expression of APP, NCT and APH-1 did not change in Prx I transfectants. Therefore, these results suggested that the expression of Prx I may be induced by the accumulation of Aβ-42 peptides and the overexpression of Prx I in neuroblastoma cells may regulate the expression of γ-secretase components.
Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang, Korea.
Young Ju
YJ
Lee
Jun Seo
JS
Goo
Ji Eun
JE
Kim
So Hee
SH
Nam
In Sik
IS
Hwang
Sun Il
SI
Choi
Hye Ryun
HR
Lee
Eon Phil
EP
Lee
Hae Wook
HW
Choi
Hong Sung
HS
Kim
Jae Ho
JH
Lee
Young Jin
YJ
Jung
Hak Jin
HJ
Kim
Dae Youn
DY
Hwang