Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery.
OBJECTIVE
In breast cancer patients dysregulation of reactive oxygen species metabolism, as detected by various indicators in plasma or various blood cells, including red blood cells and blood platelets, is observed.


METHODS
The aim of our study was to explain the effect of oxidative/nitrative stress on hemostatic activity of plasma from breast cancer patients. Different methods were used to analyze oxidative/nitrative stress (carbonyl groups and 3-nitrotyrosine levels - ELISA and C-ELISA method, respectively and free thiol groups was estimated with 5,5'-dithio-bis(2-nitro-benzoic acid)). We also measured total antioxidant status (TAS) in plasma.


RESULTS
Our results showed the correlation between the oxidative stress and changes of hemostasis in breast cancer patients; the correlation between the increased amount of carbonylated or nitrated plasma proteins and fibrin polymerization or lysis in plasma isolated from invasive breast cancer patients. We also observed that the relationship between TAS and fibrin polymerization or lysis in plasma from invasive breast cancer exists.


CONCLUSIONS
Considering the data presented in this study, we suggest that the oxidative/nitrative stress in breast cancer patients may induce the changes of hemostasis in these patients.
Department of General Biochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Magdalena
M
Kedzierska
Beata
B
Olas
Barbara
B
Wachowicz
Arkadiusz
A
Jeziorski
Janusz
J
Piekarski
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery*; D016022:Case-Control Studies; D005260:Female; D006487:Hemostasis; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D015538:Nitrosation; D018384:Oxidative Stress; D050050:Protein Carbonylation; D013438:Sulfhydryl Compounds / Q000097:blood*; D014443:Tyrosine / Q000378:metabolism*
D000975:Antioxidants; D014408:Biomarkers, Tumor; D013438:Sulfhydryl Compounds; D014443:Tyrosine