Characterization of fluorescent eye markers for mammalian transgenic studies.
Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.
Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, Connecticut, USA.
Jonathan C
JC
Cornett
Sean F
SF
Landrette
Tian
T
Xu
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D005123:Eye / Q000378:metabolism*; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic
D015415:Biomarkers; D008164:Luminescent Proteins