Tumor associated antigen specific T-cell populations identified in ex vivo expanded TIL cultures.
Ex vivo expanded tumor infiltrating lymphocytes (TILs) from malignant melanoma (MM) and head & neck squamous cell carcinoma (HNSCC) share a similar oligoclonal composition of T effector memory cells, with HLA class I restricted lysis of tumor cell lines. In this study we show that ex vivo expanded TILs from MM and HNSCC demonstrate a heterogeneous composition in frequency and magnitude of tumor associated antigen specific populations by Elispot IFNγ quantitation. TILs from MM and HNSCC shared reactivity towards NY ESO-1, cyclin B1 and Bcl-x derived peptides. Additionally we show that dominating T-cell clones and functionality persists through out expansion among an oligoclonal composition of T-cells. Our findings mirror prior results on the oligoclonal composition of TIL cultures, further indicating a potential for a broader repertoire of specific effector cells recognizing the heterogeneous tumors upon adoptive transfer; increasing the probability of tumor control by minimizing immune evasion by tumor cell escape variants.
Center for Cancer Immune Therapy, Department of Oncology, University Hospital Herlev, Herlev Ringvej 75, DK-2730 Herlev, Denmark. niejun01@heh.regionh.dk
Niels
N
Junker
Pia
P
Kvistborg
Tania
T
Køllgaard
Per thor
Pt
Straten
Mads Hald
MH
Andersen
Inge Marie
IM
Svane
D000951:Antigens, Neoplasm / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002452:Cell Count; D002470:Cell Survival / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D056744:Cyclin B1 / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008545:Melanoma / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D018709:Statistics, Nonparametric; D051020:bcl-X Protein / Q000276:immunology
D000951:Antigens, Neoplasm; C534699:CCNB1 protein, human; C104948:CTAG1B protein, human; D056744:Cyclin B1; D008565:Membrane Proteins; D051020:bcl-X Protein