Insulin immuno-neutralization in fed chickens: effects on liver and muscle transcriptome.
Chickens mimic an insulin-resistance state by exhibiting several peculiarities with regard to plasma glucose level and its control by insulin. To gain insight into the role of insulin in the control of chicken transcriptome, liver and leg muscle transcriptomes were compared in fed controls and "diabetic" chickens, at 5 h after insulin immuno-neutralization, using 20.7K-chicken oligo-microarrays. At a level of false discovery rate <0.01, 1,573 and 1,225 signals were significantly modified by insulin privation in liver and muscle, respectively. Microarray data agreed reasonably well with qRT-PCR and some protein level measurements. Differentially expressed mRNAs with human ID were classified using Biorag analysis and Ingenuity Pathway Analysis. Multiple metabolic pathways, structural proteins, transporters and proteins of intracellular trafficking, major signaling pathways, and elements of the transcriptional control machinery were largely represented in both tissues. At least 42 mRNAs have already been associated with diabetes, insulin resistance, obesity, energy expenditure, or identified as sensors of metabolism in mice or humans. The contribution of the pathways presently identified to chicken physiology (particularly those not yet related to insulin) needs to be evaluated in future studies. Other challenges include the characterization of "unknown" mRNAs and the identification of the steps or networks, which disturbed tissue transcriptome so extensively, quickly after the turning off of the insulin signal. In conclusion, pleiotropic effects of insulin in chickens are further evidenced; major pathways controlled by insulin in mammals have been conserved despite the presence of unique features of insulin signaling in chicken muscle.
Station de Recherches Avicoles, INRA, 37380 Nouzilly, France. simon@tours.inra.fr
Jean
J
Simon
Dragan
D
Milenkovic
Estelle
E
Godet
Cedric
C
Cabau
Anne
A
Collin
Sonia
S
Métayer-Coustard
Nicole
N
Rideau
Sophie
S
Tesseraud
Michel
M
Derouet
Sabine
S
Crochet
Estelle
E
Cailleau-Audouin
Christelle
C
Hennequet-Antier
Christian
C
Gespach
Tom E
TE
Porter
Michel J
MJ
Duclos
Joëlle
J
Dupont
Larry A
LA
Cogburn
D000821:Animal Feed; D000818:Animals; D057134:Antibodies, Neutralizing / Q000494:pharmacology*; D002645:Chickens / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007328:Insulin / Q000276:immunology* / Q000502:physiology; D007330:Insulin Antibodies / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D046228:Microarray Analysis; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009500:Neutralization Tests; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects*
D057134:Antibodies, Neutralizing; D007328:Insulin; D007330:Insulin Antibodies; D011506:Proteins