Evaluation of gender-related differences in various oxidative stress enzymes in mice.
Oxidative stress caused by redundant free radical, lipid oxygen and peroxide usually results in the pathogenesis of various diseases, which can be alleviated by cellular antioxidant enzymes. According to statistics, there are different incidence rates of some diseases depending on the gender. The present study aimed to investigate potential gender-related differences of antioxidant enzymes in mice. The activities of glutamate-cysteine ligase (GCL), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) in the kidney, brain, lung and heart of both male and female mice were determined. Our results showed that GPx and GCL activities were higher in female kidney and brain than those in male. On the other hand, the activities of SOD were higher in female brain and lung than those in male. Moreover, female kidney appeared to show higher activities of CAT than the male kidney. But the activities of GCL and GPx were higher in male heart than those in female. Taken together, our results demonstrate that there are gender-related differences in the activities of cellular antioxidant enzymes in various important organs in mice. Variations in such enzymes may be the explanation for some gender-related diseases.
Department of Pharmacognosy, China Pharmaceutical University Nanjing, Jiangsu, People's Republic of China.
Ying
Y
Chen
Li-Li
LL
Ji
Tian-Yu
TY
Liu
Zheng-Tao
ZT
Wang
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000201:enzymology; D018384:Oxidative Stress / Q000502:physiology*; D012727:Sex Characteristics; D013482:Superoxide Dismutase / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
D000975:Antioxidants; D004798:Enzymes; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005721:Glutamate-Cysteine Ligase; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse