Rapid development of microsatellite markers for Pandanus boninensis (Pandanaceae) by pyrosequencing technology.
OBJECTIVE
To facilitate rapid development of microsatellite or simple sequence repeat (SSR) markers, an expressed sequence tags (EST) database was constructed for Pandanus boninensis, an evergreen tree endemic to the Bonin Islands, using pyrosequencing technology.


RESULTS
We designed primers for 340 EST-SSRs identified from 109620 pyrosequencing reads, 48 of which were tested for PCR amplification. Thirty-four primers provided clear amplification, and 26 of those 34 displayed clear polymorphic patterns in sampled populations, with mean expected heterozygosity at the amplified loci ranging from 0.022 to 0.742 (average 0.262).


CONCLUSIONS
The developed markers are promising tools for future genetic studies of P. boninensis and related species.
Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan. setsukos@affrc.go.jp
Suzuki
S
Setsuko
Kentaro
K
Uchiyama
Kyoko
K
Sugai
Hiroshi
H
Yoshimaru
D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D005828:Genetics, Population; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D031673:Pandanaceae / Q000145:classification / Q000235:genetics*; D024301:Plant Bark / Q000145:classification / Q000235:genetics; D011110:Polymorphism, Genetic; D036226:Seedlings / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers