Development of a N-acetyl-β-D-glucosaminidase (NAG) assay on a centrifugal lab-on-a-compact-disc (Lab-CD) platform.
A centrifugal microfluidic platform, which is also known as lab-on-a-compact-disc (Lab-CD), was developed for use as a urinary N-acetyl-β-D-glucosaminidase (NAG) activity assay. In this work, Lab-CD design, centrifugal operations and analytical procedures were established. Automated liquid handling on Lab-CD processes, such as fluid transport, sample metering, mixing, and fluorescence detection are accomplished using a portable Lab-CD system. The linearity of the NAG assay using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-GlcNAc) was found to be acceptable in the range of 2.5 to 20 U L(-1); the relative standard deviations for the fluorescence intensity of eight samples (7.5 U L(-1)) was 6.4%. Clinical diagnostics is one of the most promising applications for Lab-CD technologies. All the benefits of miniaturization, such as reduced sample requirement, reduced reagent consumption and automation, are realized in this investigation.
Health Research Institute, National Institute of Advanced Industrial Science and Technology, Midorigaoka, Ikeda, Osaka, Japan. yoshihide-tanaka@aist.go.jp
Yoshihide
Y
Tanaka
Seira
S
Okuda
Ayumi
A
Sawai
Shigeo
S
Suzuki
D000118:Acetylglucosaminidase / Q000378:metabolism / Q000652:urine*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
D000118:Acetylglucosaminidase