[Determination of vedaprofen in livestock products and seafoods by liquid chromatography-tandem mass spectrometry].
A novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the trace residue determination of vedaprofen (VPF) in livestock products and seafoods. VPF was extracted from each sample with acidified acetone, and the crude extract was re-extracted with ethyl acetate and NaCl solution. Clean-up was performed using a weak anion exchange cartridge (Bond Elut DEA). The LC separation was performed on a C18 column using acetonitrile-0.0025 mol/L formic acid (3 : 2) as the mobile phase and MS was run in the negative ion electrospray ionization mode. The calibration curve was linear in the range of 0.001-0.1 µg/mL VPF. The mean recoveries from equine muscle, cattle muscle, cattle liver, cattle fat, salmon, eel, corbicula, milk, egg and buckwheat honey were 72-94%, and the relative standard deviations (RSDs) were 1.1-2.0%. Limits of quantitation (LOQs) ranged from 0.001 to 0.007 µg/g.
Japan Food Research Laboratories, Ibaraki-shi, Osaka, Japan.
Michiyo
M
Ishihara
Kazuhiro
K
Fujita
Hironobu
H
Ito
Takahiro
T
Matsuda
Yoh-ichi
Y
Yatsukawa
Munetomo
M
Nakamura
Takatoshi
T
Sakai
Satoru
S
Nemoto
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D004531:Eggs / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D006722:Honey / Q000032:analysis; D058751:Livestock; D008461:Meat Products / Q000032:analysis*; D008892:Milk / Q000737:chemistry; D009281:Naphthalenes / Q000032:analysis* / Q000302:isolation & purification; D011422:Propionates / Q000032:analysis* / Q000302:isolation & purification; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009281:Naphthalenes; D011422:Propionates; C121784:2-(4-cyclohexyl-1-naphthalenyl)propanoic acid