Freezing mammalian cells for production of biopharmaceuticals.
Cryopreservation techniques utilize very low temperatures to preserve the structure and function of living cells. Various strategies have been developed for freezing mammalian cells of biological and medical significance. This paper highlights the importance and application of cryopreservation for recombinant mammalian cells used in the biopharmaceutical industry to produce high-value protein therapeutics. It is a primer that aims to give insight into the basic principles of cell freezing for the benefit of biopharmaceutical researchers with limited or no prior experience in cryobiology. For the more familiar researchers, key cell banking parameters such as the cell density and hold conditions have been reviewed to possibly help optimize their specific cell freezing protocols. It is important to understand the mechanisms underlying the freezing of complex and sensitive cellular entities as we implement best practices around the techniques and strategies used for cryopreservation.
Late Stage Cell Culture, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. seth.gargi@gene.com
Gargi
G
Seth
D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D048429:Cell Size; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*
D003451:Cryoprotective Agents; D011994:Recombinant Proteins