Gene profiling of laser-microdissected brain regions and individual cells in drug abuse and schizophrenia research.
Application of transcriptomics approaches to accurately dissected anatomically defined brain regions and individual neuronal populations remains a central focus of current neurobiological investigations. A vast selection of methods and commercial products are currently available that allow one to implement routine gene quantitation and profiling from laser-microdissected brain regions, subregions, and neuronal populations. The present chapter reviews laser microdissection strategies for gene expression analyses, strategies for RNA extraction, reverse transcriptase-coupled PCR (RT-PCR), and target preparation for microarray analyses that are in use in our labs.
Department of Molecular and Integrative Neuroscience, The Scripps Research Institute, La Jolla, CA, USA. psanna@scripps.edu
Pietro Paolo
PP
Sanna
Vez
V
Repunte-Canonigo
Alessandro
A
Guidotti
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D007834:Lasers; D051379:Mice; D035683:MicroRNAs / Q000032:analysis; D042282:Microdissection / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012559:Schizophrenia / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019966:Substance-Related Disorders / Q000235:genetics*; D059467:Transcriptome
D035683:MicroRNAs; D012333:RNA, Messenger