The airway epithelium nucleotide-binding domain and leucine-rich repeat protein 3 inflammasome is activated by urban particulate matter.
BACKGROUND
The airway epithelium is the first line of defense against inhaled insults and therefore must be capable of coordinating appropriate inflammatory and immune responses.


OBJECTIVE
We sought to test the hypothesis that the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome, an intracellular danger-sensing complex, plays a critical role in airway epithelium-mediated immune responses to urban particulate matter (PM) exposure.


METHODS
In this study we (1) identified NLRP3 and caspase-1 expression in human airway epithelium bronchus and primary cells, (2) characterized NLRP3 inflammasome-mediated IL-1β production from human airway epithelium in response to PM, and (3) performed in vivo PM exposure experiments with wild-type and Nlrp3(-/-) mice.


RESULTS
Our results demonstrate that human airway epithelium contains a functional NLRP3 inflammasome that responds to PM exposure with caspase-1 cleavage and production of IL-1β. Exposure of Nlrp3(-/-) and wild-type mice to PM in vivo demonstrates NLRP3-dependent production of IL-1β in the lung, airway neutrophilia, and increases in CD11c(+hi)/MHC class II(+hi) cell numbers in intrathoracic lymph nodes.


CONCLUSIONS
Our study is the first to characterize airway epithelial NLRP3 inflammasome-mediated immune responses to PM exposure, which might have implications in patients with asthma and other lung diseases.
St Paul's Hospital, University of British Columbia, Vancouver, British Columbia, Canada. Jeremy.Hirota@hli.ubc.ca
Jeremy A
JA
Hirota
Simon A
SA
Hirota
Stephanie M
SM
Warner
Dorota
D
Stefanowicz
Furquan
F
Shaheen
Paul L
PL
Beck
Justin A
JA
Macdonald
Tillie-Louise
TL
Hackett
Don D
DD
Sin
Stephan
S
Van Eeden
Darryl A
DA
Knight
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D058847:Inflammasomes / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D000090263:Leucine-Rich Repeat Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D052638:Particulate Matter / Q000276:immunology*; D021381:Protein Transport; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000276:immunology* / Q000378:metabolism*
D002352:Carrier Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D000090263:Leucine-Rich Repeat Proteins; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D052638:Particulate Matter; D011506:Proteins