Adipose stem cell-based soft tissue regeneration.
BACKGROUND
Since their isolation and characterization nearly a decade ago, adipose-derived stem cells (ASCs) have become one of the most popular adult stem cell populations for research in soft tissue engineering and regenerative medicine applications. Compared with other stem cell sources, ASCs offer several advantages including an abundant autologous source, minor invasive harvesting (liposuction), significant proliferative capacity in culture and multi-lineage potential. Numerous preclinical studies have been pursued, with early clinical data appearing in the literature.


METHODS
Autologous fat grafting has gained tremendous momentum in clinical practice over the past several years due to its potential applications in trauma and reconstructive surgery. This review focuses on the published clinical and pre-clinical (i.e., animal) data to date using ASCs for soft tissue reconstruction, with particular attention to experimental models and methodologies. Future directions for rendering soft tissue reconstructive therapies more effective are discussed.


CONCLUSIONS
Although standardization of ASC harvesting and processing techniques, as well as long-term results of existing clinical studies, remains to be addressed, the known biological properties of ASCs suggest a potential role in enhancing fat graft retention and facilitating minimally invasive reconstructive treatments. While clinical applications are being reported, well controlled clinical studies are needed to demonstrate safety and efficacy.
University of Pittsburgh, Division of Plastic Surgery, Department of Surgery, Pittsburgh, PA 15261, USA.
Brian J
BJ
Philips
Kacey G
KG
Marra
J Peter
JP
Rubin
D017667:Adipocytes / Q000502:physiology / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D000818:Animals; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D019651:Plastic Surgery Procedures / Q000379:methods; D012038:Regeneration / Q000502:physiology*; D017695:Soft Tissue Injuries / Q000473:pathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods; D014182:Transplantation, Autologous