Glycerol metabolism alteration in adipocytes from n3-PUFA-depleted rats, an animal model for metabolic syndrome.
Aquaglyceroporin 7 (AQP7) is a glycerol transporter expressed in adipocytes. Its expression has been shown to be modulated in obesity. Metabolic syndrome is characterized by abdominal obesity, insulin resistance, dyslipidemia, and hypertension. An animal model displaying several features of metabolic syndrome was used to study the AQP7 expression at both mRNA and protein level and glycerol flux in adipocytes. Second generation n3-PUFA depleted female rats is a good animal model for metabolic syndrome as it displays characteristic features such as liver steatosis, visceral obesity, and insulin resistance. Our data show a reduced expression of AQP7 at the protein level in adipose tissue from n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-(14)C]-Glycerol uptake was not modified in adipocytes from n3-PUFA-depleted animals.
Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium.
L
L
Portois
Y
Y
Zhang
J
J
Perret
K
K
Louchami
N
N
Gaspard
E
E
Hupkens
N
N
Bolaky
V
V
Delforge
R
R
Beauwens
W J
WJ
Malaisse
A
A
Sener
Y A
YA
Carpentier
C
C
Delporte
D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005231:Fatty Acids, Unsaturated / Q000172:deficiency* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005990:Glycerol / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D050356:Lipid Metabolism; D024821:Metabolic Syndrome / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D013997:Time Factors
C491926:Aqp7 protein, rat; D020346:Aquaporins; D005231:Fatty Acids, Unsaturated; D005990:Glycerol