Tetracycline repressor-based mammalian two-hybrid systems.
The study of protein-protein interactions is critical for the understanding and regulation of biological systems. To that end, yeast two-hybrid systems have been used to study protein-protein interactions in vivo, but they frequently suffer from a high incidence of false positives when applied to mammalian systems. A novel mammalian two-hybrid system has recently been developed which exhibits lower background and higher sensitivity than earlier mammalian two-hybrid systems. It has successfully detected interactions with dissociation constants ranging from 0.99 nM to 55 μM. The system was built upon the tetracycline repressor-tetracycline operator interaction and is suitable for use in the study of most, if not all, mammalian protein-protein interactions.
College of Pharmacy, University of Texas at Austin, Austin, TX, USA.
Kathryn
K
Moncivais
Zhiwen Jonathan
ZJ
Zhang
D000818:Animals; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D017930:Genes, Reporter / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008322:Mammals / Q000235:genetics*; D008856:Microscopy, Fluorescence; D009875:Operator Regions, Genetic / Q000235:genetics; D016133:Polymerase Chain Reaction; D025941:Protein Interaction Mapping / Q000379:methods*; D012097:Repressor Proteins / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism*; D014162:Transfection
D012097:Repressor Proteins; D004262:DNA Restriction Enzymes; D013752:Tetracycline