Genetic diversity and relationship of global faba bean (Vicia faba L.) germplasm revealed by ISSR markers.
Genetic diversity and relationships of 802 faba bean (Vicia faba L.) landraces and varieties from different geographical locations of China and abroad were examined using ISSR markers. A total of 212 repeatable amplified bands were generated with 11 ISSR primers, of which 209 were polymorphic. Accessions from North China showed highest genetic diversity, while accessions from central China showed low level of diversity. Chinese spring faba bean germplasm was clearly separated from Chinese winter faba bean, based on principal component analysis and UPGMA clustering analysis. Winter accessions from Zhejiang (East China), Jiangxi (East China), Sichuan (Southwest China) and Guizhou (Southwest China) were quite distinct to that from other provinces in China. Great differentiation between Chinese accessions and those from rest of the world was shown with a UPGMA dendrogram. AMOVA analyses demonstrated large variation and differentiation within and among groups of accessions from China. As a continental geographic group, accessions from Europe were genetically closer to those from North Africa. Based on ISSR data, grouping results of accessions from Asia, Europe and Africa were obviously associated with their geographical origin. The overall results indicated that the genetic relationship of faba bean germplasm was closely associated with their geographical origin and their ecological habit.
Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.
Hai-Fei
HF
Wang
Xu-Xiao
XX
Zong
Jian-Ping
JP
Guan
Tao
T
Yang
Xue-Lian
XL
Sun
Yu
Y
Ma
Robert
R
Redden
D000704:Analysis of Variance; D002681:China; D016000:Cluster Analysis; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D013045:Species Specificity; D031307:Vicia faba / Q000145:classification* / Q000235:genetics*