[Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria].
Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-CO-4HB), the most promising member of the biodegradable polyhydroxyalcanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-CO-4HB copolymer. A set of highly pure samples of the 3HB-CO-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol % has been obtained. Incorporation of 4-HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-CO-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa).
T G
TG
Volova
N O
NO
Zhila
G S
GS
Kalacheva
V A
VA
Sokolenko
A J
AJ
Sinskey
D018929:Cell Culture Techniques; D003461:Crystallography; D003470:Culture Media / Q000737:chemistry; D020118:Cupriavidus necator / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D003470:Culture Media; D006859:Hydrogen; D006885:Hydroxybutyrates; D011091:Polyesters; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates