Soft-food diet induces oxidative stress in the rat brain.
Decreased dopamine (DA) release in the hippocampus may be caused by dysfunctional mastication, although the mechanisms involved remain unclear. The present study examined the effects of soft- and hard-food diets on oxidative stress in the brain, and the relationship between these effects and hippocampal DA levels. The present study showed that DA release in the hippocampus was decreased in rats fed a soft-food diet. Electron spin resonance studies using the nitroxyl spin probe 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl directly demonstrated a high level of oxidative stress in the rat brain due to soft-food diet feeding. In addition, we confirmed that DA directly react with reactive oxygen species such as hydroxyl radical and superoxide. These observations suggest that soft-food diet feeding enhances oxidative stress, which leads to oxidation and a decrease in the release of DA in the hippocampus of rats.
Division of Pharmacology & ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Yokosuka 238-8580, Japan.
Fumihiko
F
Yoshino
Ayaka
A
Yoshida
Norio
N
Hori
Yumie
Y
Ono
Katsuhiko
K
Kimoto
Minoru
M
Onozuka
Masaichi Chang-il
MC
Lee
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D003497:Cyclic N-Oxides; D004032:Diet / Q000379:methods*; D004298:Dopamine / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006624:Hippocampus / Q000166:cytology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011759:Pyrrolidines; D051381:Rats; D017208:Rats, Wistar
D003497:Cyclic N-Oxides; C109806:MCPROXYL; D011759:Pyrrolidines; D004298:Dopamine