Light-triggered conversion of non-ionic into ionic surfactants: towards chameleon detergents for 2-D gel electrophoresis.
Caged non-ionic detergents, comprised of polar oligo(ethylene glycol) and non-polar alkyl chains joined by a photocleavable ortho-nitrobenzyl sulfonate linker have been synthesized and characterized. The light-triggered transformation of such chameleon surfactant from a charge-neutral into a charged form offers great potential to improve 2-D gel electrophoretic separation of complex protein mixtures.
Institut für Chemie-Biochemie, Freie Universität Berlin, Berlin, Germany.
Oliver
O
Bogen
Petar
P
Milosevic
Hans
H
Daroszi
Mathias
M
Dreger
Stefan
S
Hecht
Ferdinand
F
Hucho
D015180:Electrophoresis, Gel, Two-Dimensional; D019855:Ethylene Glycol / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D008027:Light; D015394:Molecular Structure; D010782:Photolysis; D012995:Solubility; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
D007477:Ions; D013501:Surface-Active Agents; D019855:Ethylene Glycol