[The effect of total phenolics from abnormal savda munziq on cervical cancer cell lines].
OBJECTIVE
To explore the feasibility of total phenolics from Abnormal Savda Munziq on treating human cervical carcinoma.


METHODS
MTT assay was used to determine the growth inhibition ratio and IC50 on Hela cells and SiHa cells after treated with different concentrations of total phenolics. Morphologic changes of cells were observed by inverted microscope.


RESULTS
Total phenolics inhibited the proliferation of Hela cells in the concentration range of 25 - 125 microg/mL; Total phenolics inhibited the proliferation of SiHa cells in the concentration range of 75 - 175 microg/mL; The inhibitory actions of total phenolics on the Hela and SiHa appeard dose-effect relationship and time-effect relationship obviously (P < 0.01); The IC50 of Hela was (125.26 +/- 16.15) microg/mL after 48h total phenolics treatment; The IC50 of SiHa was (134.51 +/- 2.55) microg/mL after 48h total phenolics treatment. And there was no statistical sense in the disparation of them (P > 0.05); Both of the cells showed apoptosis character evidently after total phenolics treatment, Along with the increasing of the concentration and the action time, morphologic changes of cells were more obviously.


CONCLUSIONS
Total phenolics could inhibit the growth of Hela cells and SiHa cells and the inhibitory actions of total phenolics on the two cells is almostly the same. Therefore, total phenolics from abnormal savda munziq is deserved to be further studied for treating human cervical carcinoma.
Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China. 656262393@qq.com
Ke-Yao
KY
Chen
Ablimit
A
Tangnur
Guo-Ning
GN
Wang
Abliz
A
Guzalnur
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010636:Phenols / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; D010636:Phenols