Molecular and phenotypic characterization of strains nodulating Anthyllis vulneraria in mine tailings, and proposal of Aminobacter anthyllidis sp. nov., the first definition of Aminobacter as legume-nodulating bacteria.
Bacterial strains from Zn-Pb mine tailings were isolated by trapping with Anthyllis vulneraria, a legume-host suitable for mine substratum phytostabilisation. Sequence analysis of the 16S rRNA gene and three housekeeping genes (atpD, dnaK and recA) showed that they were related to those of the genus Aminobacter. DNA-DNA relatedness of representative isolates supported the placement of novel strains in Aminobacter as a new species. Phenotypic data emphasize their differentiation from the other related species of Aminobacter and Mesorhizobium. Aminobacter isolates exhibited nodA sequences tightly related with M. loti as the closest nodA relative. By contrast, their nodA sequences were highly divergent from those of M. metallidurans, another species associated with A. vulneraria that carries two complete copies of nodA. Therefore, the novel bacterial strains efficient on A. vulneraria represented the first occurrence of legume symbionts in the genus Aminobacter. They represent a new species for which the name Aminobacter anthyllidis sp. nov. is proposed (type strain STM4645(T)=LMG26462(T)=CFBP7437(T)).
INRA, USC1242, Laboratoire des Symbioses Tropicales et Méditerranéennes IRD/INRA/CIRAD/Montpellier SupAgro/Université Montpellier II, TA A-82/J, F-34398, Montpellier Cedex 5, France.
Géraldine
G
Maynaud
Anne
A
Willems
Souhir
S
Soussou
Céline
C
Vidal
Lucette
L
Mauré
Lionel
L
Moulin
Jean-Claude
JC
Cleyet-Marel
Brigitte
B
Brunel
D000217:Acyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D007854:Lead; D008906:Mining; D008969:Molecular Sequence Data; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology; D017422:Sequence Analysis, DNA; D015032:Zinc
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D007854:Lead; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; D011938:Rec A Recombinases; D015032:Zinc