Functionalized quantum dots with dopamine dithiocarbamate as the matrix for the quantification of efavirenz in human plasma and as affinity probes for rapid identification of microwave tryptic digested proteins in MALDI-TOF-MS.
Functionalized quantum dots with dopamine dithiocarbamate (QDs-DDTC) were utilized for the first time as an efficient material for the quantification of efavirenz in human plasma of HIV infected patients and rapid identification of microwave tryptic digest proteins (cytochrome c, lysozyme and BSA) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The synthesized QDs-DDTC was characterized by using spectroscopic (UV-visible, FT-IR and (1)H NMR) and microscopic (SEM and TEM) techniques. Functionalized QDs-DDTC exhibited a high desorption/ionization efficiency for the rapid quantification of small molecules (efavirenz, tobramycin and aspartame) at low-mass region. QDs-DDTC has well ability to trap target species, and capable to transfer laser energy for efficient desorption/ionization of analytes with background-free detection. The use of QDs-DDTC as a matrix provided good linearity for the quantification of small molecules (R(2)=~0.9983), with good reproducibility (RSD<10%), in the analysis of efavirenz in the plasma of HIV infected patients by the standard addition method. We also demonstrated that the use of functionalized QDs-DDTC as affinity probes for the rapid identification of microwave tryptic digested proteins (cytochrome c, lysozyme and BSA) by MALDI-TOF-MS. QDs-DDTC-based MALDI-TOF-MS approach provides simplicity, rapidity, accuracy, and precision for the determination of efavirenz in human plasma of HIV infected patients and rapid identification of microwave tryptic digested proteins. This new material presents a marked advance in the development of matrix-free mass spectrometric methods for the rapid and precise quantitative determination of a variety of molecules. This article is part of a Special Issue entitled: Proteomics: The clinical link.
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan.
Suresh Kumar
SK
Kailasa
Hui-Fen
HF
Wu
D000345:Affinity Labels / Q000737:chemistry / Q000494:pharmacology; D000480:Alkynes; D019380:Anti-HIV Agents / Q000032:analysis / Q000097:blood; D048588:Benzoxazines / Q000032:analysis* / Q000097:blood; D003521:Cyclopropanes; D004298:Dopamine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008872:Microwaves; D008954:Models, Biological; D011506:Proteins / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D059748:Proteolysis / Q000528:radiation effects; D040901:Proteomics / Q000379:methods; D045663:Quantum Dots; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013859:Thiocarbamates / Q000737:chemistry*; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
D000345:Affinity Labels; D000480:Alkynes; D019380:Anti-HIV Agents; D048588:Benzoxazines; D003521:Cyclopropanes; D011506:Proteins; D013859:Thiocarbamates; C573437:dopamine dithiocarbamate; D014357:Trypsin; C098320:efavirenz; D004298:Dopamine