Simultaneous quantification of five marker compounds of Betula utilis stem bark using a validated high-performance thin-layer chromatography method.
A sensitive, selective and robust densitometric high-performance thin-layer chromatographic method was developed and validated for five marker compounds, namely betulin, lupeol, oleanolic acid, 3-acetyloleanolic acid and β-sitosterol, known for their various therapeutic activities. The marker compounds have been isolated from the stem bark of Betula utilis, well characterized by the spectral analysis, and their simultaneous quantitative determination carried out by high-performance thin-layer chromatography (HPTLC) method. The resolution of marker compounds was carried out on silica-gel 60 plates, using n-hexane:ethyl acetate (8:2 v/v) as the mobile phase. The HPTLC densitometry was performed at 500-nm wavelength after the post chromatographic derivatization with ceric ammonium sulfate reagent. The optimized method provided good linear relation (r>0.9960) for all the investigated analytes. The method is simple, and reproducible, which may be applied for quantitative analysis of the above-mentioned marker compounds.
Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Jammu Tawi, India.
Imran
I
Khan
Payare L
PL
Sangwan
Jagdish K
JK
Dhar
Surrinder
S
Koul
D029662:Betula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009828:Oleanolic Acid / Q000032:analysis; D053978:Pentacyclic Triterpenes / Q000032:analysis; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry; D012855:Sitosterols / Q000032:analysis; D014315:Triterpenes / Q000032:analysis; D000094062:Betulinic Acid; D000094042:Lupanes
D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012855:Sitosterols; D014315:Triterpenes; C052658:oleanolic acid 3-acetate; C025473:gamma-sitosterol; C002503:betulin; D000094062:Betulinic Acid; C010480:lupeol; D000094042:Lupanes