Assessment of lifestyle effect on oxidative stress biomarkers in free-living elderly in rural Japan.
BACKGROUND
Oxidative stress is believed to play a crucial role in aging and age-related diseases, and is widely thought to increase morbidity and mortality in the elderly. Assessment of biomarkers of oxidative stress, such as 8-isoprostane and 8-hydroxy-2-deoxyguanosine, are considered to be useful in predicting disease risks at the population level.


OBJECTIVE
The aim of the present study was to assess the health status of the elderly by comparing their lifestyles and levels of oxidative stress biomarkers.


METHODS
We carried out a cross-sectional study where urine samples from a total of 100 elderly men and women were assayed for 8-isoprostane, 8-hydroxy-2-deoxyguanosine, selenium, cadmium and creatinine. They were asked to answer a questionnaire that included questions about their lifestyle.


RESULTS
Most of the participants were prehypertensive, non-alcohol users and on a rich plant-based diet. There were no differences in any biomarkers of oxidative stress between men and women. 8-Isoprostane was found to correlate positively with systolic blood pressure in women, but not in men. There was a slight increase of 8-isoprostane in participants with a poor intake of vegetables, and a decrease of 8-hydroxy-2-deoxyguanosine in participants who consumed fish. Multiple regression analysis showed that oxidative stress biomarkers were positively associated with cadmium, and negatively associated with selenium and fish intake in all participants, 89% of which were non-smokers.


CONCLUSIONS
Results from the present study show that fish intake has the potential of decreasing oxidative stress among elderly persons.
Division of Social Medicine, Department of Environmental Medicine, Kochi Medical School, Kochi University, Kochi, Japan. andersonbasilua@yahoo.fr
Basilua Andre
BA
Muzembo
Dumavibhat
D
Narongpon
N'landu Roger
NR
Ngatu
Masamitsu
M
Eitoku
Ryoji
R
Hirota
Narufumi
N
Suganuma
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000203:Activities of Daily Living; D000368:Aged; D000428:Alcohol Drinking / Q000453:epidemiology; D015415:Biomarkers / Q000652:urine*; D001794:Blood Pressure; D002104:Cadmium / Q000652:urine; D003401:Creatine / Q000652:urine; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004032:Diet; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006305:Health Status Indicators; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008019:Life Style; D008297:Male; D018384:Oxidative Stress; D011237:Predictive Value of Tests; D012044:Regression Analysis; D012424:Rural Population; D012643:Selenium / Q000652:urine; D012907:Smoking / Q000453:epidemiology
D015415:Biomarkers; D002104:Cadmium; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine; D012643:Selenium; D003401:Creatine