Transient expression of mouse pro-α3(V) collagen gene (Col5a3) in wound healing.
The α3(V) chain is poorly characterized among type V collagen chains. Pro-α3(V) collagen is expressed in newly synthesized bone as well as in the superficial fascia of developing muscle. Present study examined the expression in a mouse model of wound healing. Real-time reverse transcriptase polymerase chain reaction and in situ hybridization revealed transient expression of pro-α3(V) chain at a lower level than other fibrillar collagen genes after injury. Immunohistochemistry showed a similar expression pattern in the injured skin. In addition, electron microscopy showed that pro-α3(V) chain was localized in the amorphous nonfibrillar region, but not in fine or dense fibrils. The pro-α3(V) chain co-localized with heparan sulfate, which appeared in the skin after injury and might bind via an acidic segment of the pro-α3(V) chain. The matrix containing the pro-α3(V) chain may therefore be needed for the initiation of wound healing.
Department of Matrix Medicine, Faculty of Medicine, Oita University, Yufu, Oita 879-5593, Japan.
Hideaki
H
Sumiyoshi
Hirokazu
H
Kitamura
Noritaka
N
Matsuo
Shuji
S
Tatsukawa
Kazushi
K
Ishikawa
Osamu
O
Okamoto
Yoshihisa
Y
Fujikura
Sakuhei
S
Fujiwara
Hidekatsu
H
Yoshioka
D000818:Animals; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D014945:Wound Healing / Q000235:genetics*
C578895:Col5a3 protein, mouse; D011498:Protein Precursors; D012333:RNA, Messenger; C406808:pro-alpha3(V) collagen; D003094:Collagen