Identification of Chromera velia by fluorescence in situ hybridization.
Chromera velia is evolutionarily the closest free-living and photosynthetic organism to the medically important obligatory parasitic apicomplexans that cause diseases including malaria and toxoplasmosis. In this study, a novel oligonucleotide probe targeting C. velia's small subunit ribosomal RNA was designed. To enable usage of this probe as a detection tool, a fluorescence in situ hybridization (FISH) protocol was optimized. The results obtained showed that when used in combination, the C. velia CV1 probe and optimized FISH protocol enabled efficient detection of C. velia in culture. This new technique will allow a better understanding of the ecological role of C. velia within the coral microhabitat.
Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia.
Victoria
V
Morin-Adeline
Christie
C
Foster
Jan
J
Slapeta
D000818:Animals; D037421:Anthozoa / Q000469:parasitology*; D016782:Apicomplexa / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000235:genetics; D016415:Sequence Alignment
D003470:Culture Media; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D004247:DNA