Protective effect of apocynin in an established alcoholic steatohepatitis rat model.
Apocynin is a widely used antioxidant in both basic and clinical research. The current study aimed to investigate the protective effect of apocynin in an established alcoholic steatohepatitis rat model. Healthy SD rats were gastrically fed with ethanol (4.0 g/kg) for 8 weeks to induce alcoholic steatohepatitis. After 8 weeks, rats were fed with ethanol for another 2 weeks with or without a daily intraperitoneal injection of 25 mg/kg apocynin. After sacrificing, serum and liver samples were subjected to hepatic injury measurements. After 8-week ethanol induction, rats exhibited typical alcoholic steatohepatitis features including reduced body weight, hepatic histological changes, elevated serum alanine transaminase (ALT) level, increased levels of oxidative stress and inflammatory markers, NADPH oxidases, and renin-angiotensin system (RAS) marker. Co-treatment of apocynin alleviated the hepatic injury and biochemical parameters induced by alcoholic steatohepatitis. In conclusion, addition of apocynin significantly attenuates hepatic oxidative stress and inflammation induced by alcoholic steatohepatitis. This effect is partly through the inhibition of the RAS system.
Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China. fanrengen2011@qq.com
Rengen
R
Fan
Xiangxiang
X
Shan
Haixin
H
Qian
Changzhi
C
Song
Guangzhou
G
Wu
Yan
Y
Chen
Yufeng
Y
Miao
Wenzhang
W
Cha
D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002492:Central Nervous System Depressants / Q000009:adverse effects / Q000494:pharmacology; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology; D005235:Fatty Liver, Alcoholic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000187:drug effects; D013997:Time Factors
D000098:Acetophenones; D000975:Antioxidants; D015415:Biomarkers; D002492:Central Nervous System Depressants; D000431:Ethanol; C056165:acetovanillone; D019255:NADPH Oxidases