Differential induction of stearoyl-CoA desaturase 1 and 2 genes by fibrates in the liver of rats.
The administration of fibrates (fenofibrate, bezafibrate and clofibric acid) to rats induced stearoyl-CoA desaturase (SCD) in the liver, and increased relative expression of mRNAs encoding SCD1 and SCD2 in dose- and time-dependent manners. The magnitudes of the increases in SCD2 mRNA level caused by fenofibrate and clofibric acid were much higher than those of SCD1 at relatively higher doses of the fibrates, and a relatively long time (7 or 14 d) was required for significant induction of SCD2 mRNA expression compared with that of SCD1. Although the absolute number of transcripts for SCD2 was 1,800 times lower than that of SCD1 in the control liver, it was strikingly increased by fibrates. These results suggest that differential regulations operate for the gene expression between SCD1 and SCD2, and that the physiological significance of SCD2 is distinct from that of SCD1 in the liver.
Faculty of Pharmaceutical Sciences, Josai University, 1–1 Keyakidai, Sakado, Saitama 350–0295, Japan.
Tohru
T
Yamazaki
Hitomi
H
Okada
Takeshi
T
Sakamoto
Katsuyoshi
K
Sunaga
Tadashi
T
Tsuda
Atsushi
A
Mitsumoto
Naomi
N
Kudo
Yoichi
Y
Kawashima
D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology*; D002995:Clofibric Acid / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D020536:Enzyme Activators / Q000494:pharmacology*; D011345:Fenofibrate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*
D020536:Enzyme Activators; D012333:RNA, Messenger; D002995:Clofibric Acid; D013230:Stearoyl-CoA Desaturase; D011345:Fenofibrate; D001629:Bezafibrate