MBL2, MASP2, AMELX, and ENAM gene polymorphisms and dental caries in Polish children.
OBJECTIVE
The aim of the study was to examine whether the MBL2 C(-290)G and G161A, MASP2 A359G, AMELX C287T and C522T, and ENAM C2452T polymorphisms are associated with dental caries.


METHODS
Genomic DNA of 95 Polish children with 'higher caries experience' (HC) and 84 subjects with 'lower caries experience' (LC) belonging to two age-groups (5 and 13 years old) was extracted from the buccal mucosa. SNPs were genotyped with PCR-RFLP methods.


RESULTS
Among 5-year-old children, we found significantly higher percentage of subjects carrying MBL2 (-290)G allele in HC group compared with LC group (43.2%vs 17.6%, P = 0.023). MBL2 C(-290)G-G161A C-G haplotype was overrepresented in LC group in 5-year-olds (P = 0.01), while the opposite association was observed in 13-year-olds, where C-G was overrepresented in HC group (P = 0.028). In 5-year-old children, the frequency of MBL2 G-G haplotype was higher in HC group compared with LC subjects (P = 0.045), while the opposite association (with borderline significance) was observed in 13-year-old children (P = 0.057). SNPs in MASP2, AMELX, and ENAM were not associated with dental caries.


CONCLUSIONS
MBL2 gene polymorphism is associated with caries experience in Polish children, but the direction of this association seems to be opposite in primary and permanent dentition.
Department of Hygiene, Epidemiology and Public Health, Pomeranian Medical University, Szczecin, Poland. olsza@sci.pam.szczecin.pl
T
T
Olszowski
G
G
Adler
J
J
Janiszewska-Olszowska
K
K
Safranow
M
M
Kaczmarczyk
D000225:Adenine; D000293:Adolescent; D000483:Alleles; D053523:Amelogenin / Q000235:genetics*; D016022:Case-Control Studies; D002675:Child, Preschool; D003596:Cytosine; D004245:DMF Index; D003731:Dental Caries / Q000235:genetics*; D016326:Extracellular Matrix Proteins; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006147:Guanine; D006239:Haplotypes / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D011044:Poland; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D013941:Thymine
C507199:AMELX protein, human; D053523:Amelogenin; C550525:ENAM protein, human; D016326:Extracellular Matrix Proteins; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D011506:Proteins; D006147:Guanine; D003596:Cytosine; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases; D000225:Adenine; D013941:Thymine