Propofol decreases the axonal excitability in rat primary sensory afferents.
OBJECTIVE
The aim of this present study was to investigate the changes of peripheral sensory nerve excitability produced by propofol.


METHODS
In a recently described in vitro model of rodent saphenous nerve we used the technique of threshold tracking (QTRAC®) to measure changes of axonal nerve excitability of Aβ-fibres caused by propofol. Concentrations of 10 μMol, 100 μMol and 1000 μMol were tested. Latency, peak response, strength-duration time constant (τSD) and recovery cycle of the sensory neuronal action potential (SNAP) were recorded.


RESULTS
Our results have shown that propofol decreases nerve excitability of rat primary sensory afferents in vitro. Latency increased with increasing concentrations (0μMol: 0.96 ± 0.07ms; 1000μMol 1.10 ± 0.06ms, P<0.01). Also, propofol prolonged the relative refractory period (0μMol: 1.79 ± 1.13ms; 100 μMol: 2.53 ± 1.38ms, P<0.01), and reduced superexcitability (0 μMol: -14.0±4.0%; 100μMol: -9.5 ± 5.5%) and subexcitability (0μMol: 7.5 ± 1.2%; 1000μMol: 3.6 ± 1.2) significantly during the recovery cycle (P<0.01).


CONCLUSIONS
Our results have shown that propofol decreases nerve excitability of primary sensory afferents. The technique of threshold tracking revealed that axonal voltage-gated ion channels are significantly affected by propofol and therefore might be at least partially responsible for earlier described analgesic effects.
Institute of Anesthesiology, University Hospital of Zurich, Switzerland.
Lisa
L
Neukom
Nisha
N
Vastani
Burkhardt
B
Seifert
Donat R
DR
Spahn
Konrad
K
Maurer
D000200:Action Potentials / Q000187:drug effects; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001369:Axons / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D009431:Neural Conduction / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects*; D017933:Peripheral Nervous System / Q000187:drug effects; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012684:Sensory Thresholds / Q000187:drug effects*; D015222:Sodium Channels / Q000187:drug effects
D018686:Anesthetics, Intravenous; D015222:Sodium Channels; D015742:Propofol