Porous ceramic/agarose composite adsorbents for fast protein liquid chromatography.
Porous ceramic/agarose composite adsorbents were designed and prepared with silica ceramic beads and 4% agarose gel, and then functionalized with a special ligand carboxymethyl. A novel method was introduced to fabricating of the porous silica ceramic beads. The morphology of SEM shows a spherical shape and a porous structure of the ceramic beads. Nitrogen adsorption-desorption analysis gives an average pore size of 287.5 Å, a BET surface area of 29.33 m²/g and a porosity of 41.8%, respectively. Additionally, X-ray diffraction pattern indicates that the amorphous silica has been transformed into two crystal phases of quartz and cristobalite, leading to a porous and rigid skeleton and ensuring the application of the composite beads at high flow velocities. Lysozyme of hen egg-white with the activity of 12,700 U/mg was purified by the composite ion-exchanger in one step and the recovery and purification factor reaches 95.2% and 7.9, respectively.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China. hfxia@jiangnan.edu.cn
Haifeng
H
Xia
Xionghua
X
Jin
Puqiang
P
Wu
Zhiyong
Z
Zheng
D000327:Adsorption; D000818:Animals; D002516:Ceramics / Q000737:chemistry*; D002645:Chickens; D002853:Chromatography, Liquid / Q000191:economics / Q000295:instrumentation*; D008024:Ligands; D009113:Muramidase / Q000302:isolation & purification*; D010316:Particle Size; D016062:Porosity; D012685:Sepharose / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
D008024:Ligands; D012822:Silicon Dioxide; D012685:Sepharose; D009113:Muramidase