[Application of spectral karyotyping to cytogenetic analysis in acute myeloid leukemia].
OBJECTIVE
To evaluate the value of spectral karyotyping (SKY) in cytogenetic analysis of acute myeloid leukemias (AML).


METHODS
Nine AML patients were analyzed by R-banding and SKY. MLL, PML-RARalpha, AML1-ETO fusion genes were detected by dual fusion- fluorescence in situ hybridization (D-FISH).


RESULTS
All 9 samples were successfully hybridized. SKY identified structural aberrations including 9q -, t(15;17) and ins(10;17) (q22;p11p12) ; and some numeral abnormalities. The results of SKY confirmed those of R-band karyotyping and D-FISH; with more accurate localization.


CONCLUSIONS
SKY appears to be fairly stable, accurate and sensitive, for AML cytogenetic study.
Department of Geriatric Hematology, General Hospital of PLA, Beijing 100853, China.
Bo
B
Guo
Hong-Li
HL
Zhu
Su-Xia
SX
Li
Xiao-Ping
XP
Han
Jing-Fen
JF
Sun
Li-Li
LL
Wang
Wen-Rong
WR
Huang
Wan-Ming
WM
Da
D000328:Adult; D000368:Aged; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D032681:Spectral Karyotyping; D055815:Young Adult