The p65 subunit of NF-κB and PARP1 assist Snail1 in activating fibronectin transcription.
Snail1 is a transcriptional repressor of E-cadherin that triggers epithelial-mesenchymal transition (EMT). Here, we report assisted Snail1 interaction with the promoter of a typical mesenchymal gene, fibronectin (FN1), both in epithelial cells undergoing EMT and in fibroblasts. Together with Snail1, the p65 subunit of NF-κB and PARP1 bound to the FN1 promoter. We detected nuclear interaction of these proteins and demonstrated the requirement of all three for FN1 transcription. Moreover, other genes involved in cell movement mimic FN1 expression induced by Snail1 or TGF-β1 treatment and recruit p65NF-κB and Snail1 to their promoters. The molecular cooperation between Snail1 and NF-κB in transcription activation provides a new insight into how Snail1 can modulate a variety of cell programs.
Programa de Recerca en Càncer, IMIM-Hospital del Mar, E-08003, Barcelona,  Spain.
Jelena
J
Stanisavljevic
Montserrat
M
Porta-de-la-Riva
Raquel
R
Batlle
Antonio García
AG
de Herreros
Josep
J
Baulida
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D000071250:Snail Family Transcription Factors; D051996:Transcription Factor RelA / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C000604407:SNAI1 protein, human; C000604429:Snai1 protein, mouse; D000071250:Snail Family Transcription Factors; D051996:Transcription Factor RelA; D014157:Transcription Factors; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases