Effects of 4',7-dimethoxyflavanone on cell cycle arrest and apoptosis in human breast cancer MCF-7 cells.
The present study was designed to investigate the anticancer activity of 4,7-dimethoxyflavanone in vitro. When human breast cancer MCF-7 cells were treated with 4',7-dimethoxyflavanone at various concentrations (1-200 μM) for 24 h, antiproliferative effects were first observed at 1 μM and the IC(50) was 115.62 μM. Conversely, 4',7-dimethoxyflavanone was not cytotoxic (measured as lactate dehydrogenase release in CHO-K1 cells) under the same conditions. MCF-7 cells exposed to the 4',7-dimethoxyflavanone at the IC(50) concentration showed cell cycle arrest and apoptosis. Compared to the respective control level, exposure to 4',7-dimethoxyflavanone resulted in a remarkable increase of small DNA fragments at the sub-G1 phase and an increase in the G2/M phase cell population. Moreover, when 4',7-dimethoxyflavanone treatment caused G2/M phase arrest, an increase in CDK1 together with an increase in cyclin B was observed. Based on these results, 4',7-dimethoxyflavanone may be a useful anticancer agent.
Plant Resources Research Institute, Duksung Women's University, Seoul, 132-714, Korea.
Eun Jeong
EJ
Choi
Jae In
JI
Lee
Gun-Hee
GH
Kim
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D016466:CHO Cells; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D044950:Flavanones / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D016896:Treatment Outcome
D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones