Evaluation of the efficacy of a recombinant Entamoeba histolytica cysteine proteinase gene (EhCP5) antigen in Minipig.
Entamoeba histolytica cysteine proteinase gene 5(EhCP5) is one of the major proteinase genes of all EhCP-transcripts. The amebiasis cysteine proteinase gene encoding an antigen from E. histolytica, as well as the recombinant EhCP5, obtained by cloning and expression of the EhCP5 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in Minipig against challenge infection in a minipig-E. histolytica model. There was a 52.27% reduction (P<0.001) in the group of recovery of challenged E. histolytica compared with that in the control group. Specific anti-EhCP5 antibodies from immune protected minipig had significantly higher levels of immunoglobulin G (IgG) (P<0.0001). Our data will help to know the mechanism of vaccinal protection of E. histolytica.
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China. heguangzhi7711@163.com
Guang-Zhi
GZ
He
Shu-Xuan
SX
Deng
Wei-Yi
WY
Tian
Yong
Y
Feng
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D057056:Cysteine Proteases / Q000235:genetics* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D004749:Entamoebiasis / Q000276:immunology / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D016366:Open Reading Frames / Q000502:physiology; D016052:Protozoan Vaccines / Q000276:immunology / Q000592:standards*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013556:Swine, Miniature
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D007074:Immunoglobulin G; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D057056:Cysteine Proteases