Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization.
We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.
Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA 50011, USA.
Changbei
C
Ma
Edward S
ES
Yeung
Shengda
S
Qi
Rui
R
Han
D015374:Biosensing Techniques / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D035683:MicroRNAs / Q000032:analysis* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism; D058105:Polymerization
D035683:MicroRNAs; D009713:Nucleotidyltransferases; C022428:poly U polymerase