Construction of a single polypeptide that matures and exports the lasso peptide microcin J25.
Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter. We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.
Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544, USA.
Si Jia
SJ
Pan
Jakub
J
Rajniak
Wai Ling
WL
Cheung
A James
AJ
Link
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D016415:Sequence Alignment
D001430:Bacteriocins; D010455:Peptides; C020411:microcin