Lymphocyte proliferation stimulated by activated Cebus apella macrophages treated with a complex homeopathic immune response modifiers.
BACKGROUND
Canova is a complex homeopathic medicine that enhances a specific immunologic responses against several exogenous and endogenous conditions. Canova activates macrophages both in vivo and in vitro.


OBJECTIVE
We evaluated the effects of macrophages activated by Canova in vivo and ex vitro in the proliferation of lymphocytes. Canova was used to activate Cebus apella macrophages in vivo or ex vitro with Canova. Lymphocytes were cultured with the macrophage culture medium. The analysis of Canova effects in cultured lymphocytes was performed according to the cell cycle phase using flow cytometry. The Interferon gamma and Interleukin-5 cytokines quantification in these lymphocyte culture media was performed by Enzyme-linked immunosorbent assay (ELISA).


RESULTS
We observed that Canova actives macrophages in vivo and ex vitro. The lymphocytes cultured in a supplemented medium with macrophages activated by Canova treatment presented a higher number of proliferation cells than lymphocytes not exposed to macrophages activated by Canova. The Interferon gamma and Interleukin-5 cytokines were only observed in the medium of lymphocytes exposed to macrophages activated by Canova. Thus, Canova has potential as a new adjuvant therapy.
Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
Carlos Onete
CO
Coelho Moreira
Joana
J
de Fátima Ferreira Borges da Costa
Mariana Ferreira
MF
Leal
Edilson
E
Ferreira de Andrade
Alexandre Pingarilho
AP
Rezende
Aline Amaral
AA
Imbeloni
José Augusto
JA
Pereira Carneiro Muniz
Marília
M
de Arruda Cardoso Smith
Rommel Rodríguez
RR
Burbano
Paulo Pimentel
PP
de Assumpção
D000818:Animals; D002428:Cebus; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006705:Homeopathy; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008264:Macrophages; D008297:Male; D011897:Random Allocation; D016896:Treatment Outcome
D007155:Immunologic Factors; D015848:Interleukin-5; D007371:Interferon-gamma