E-cadherin and 5-HT alterations in the heart of rats having undergone             atropine-induced toxicity.
Atropine-induced heart damage is associated with changes in the expression of various enzymes and proteins. The purpose of this study was to investigate atropine-induced alterations in cardiac E-cadherin and 5-hydroxytryptamine (5-HT) after atropine administration. Male Wistar rats were randomly divided into two groups: a control group and an atropine group. The atropine group intraperitoneally received atropine at a single dose of 15 mg/kg for 7 days; the controls received the same amount of saline via the same route. On Day 8, the rats were anesthetized, and a thoracotomy was performed in all animals. Immunohistochemical analysis was performed to evaluate protein expression of E-cadherin and 5-HT. Sections were analyzed by digital image analysis. Cardiac protein expression of E-cadherin and 5-HT was altered after atropine‑induced toxicity in the rat. The expression levels of E-cadherin and 5-HT were significantly decreased after atropine treatment, supported by IOD analysis, when compared with the control (P<0.05). The current findings indicate that such changes would be reflected in abnormal cardiac function, and these proteins may be useful for revealing the mechanisms underlying atropine-induced toxicity and may also provide various clues for further research.
Department of Pathology, School of Basic Medical Sciences, Dali University, Dali, Yunnan 671000, PR China. hqy0726@126.com
Quan-Yong
QY
Huang
Xue-Fang
XF
Li
Shui-Ping
SP
Liu
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000633:toxicity*; D001285:Atropine / Q000633:toxicity*; D015820:Cadherins / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*
D000889:Anti-Arrhythmia Agents; D015820:Cadherins; D012701:Serotonin; D001285:Atropine