A simple drug anchoring microfiber scaffold for chondrocyte seeding and proliferation.
The structural properties of microfiber meshes made from poly(2-hydroxyethyl methacrylate) (PHEMA) were found to significantly depend on the chemical composition and subsequent cross-linking and nebulization processes. PHEMA microfibres showed promise as scaffolds for chondrocyte seeding and proliferation. Moreover, the peak liposome adhesion to PHEMA microfiber scaffolds observed in our study resulted in the development of a simple drug anchoring system. Attached foetal bovine serum-loaded liposomes significantly improved both chondrocyte adhesion and proliferation. In conclusion, fibrous scaffolds from PHEMA are promising materials for tissue engineering and, in combination with liposomes, can serve as a simple drug delivery tool.
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Michala
M
Rampichová
Lenka
L
Martinová
Eva
E
Koštáková
Eva
E
Filová
Andrea
A
Míčková
Matěj
M
Buzgo
Jiří
J
Michálek
Martin
M
Přádný
Alois
A
Nečas
David
D
Lukáš
Evžen
E
Amler
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D015195:Drug Design; D008081:Liposomes / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D008081:Liposomes; D011108:Polymers; D011102:Polyhydroxyethyl Methacrylate