Analysis of the coding sequence and expression of the coiled-coil α-helical             rod protein 1 gene in normal and neoplastic epithelial cervical cells.
The role of the CCHCR1 (coiled-coil α-helical rod protein 1) protein in the cell is poorly understood. It is thought to be engaged in processes such as proliferation and differentiation of epithelial cells, tissue-specific gene transcription and steroidogenesis. It is supposed to participate in keratinocyte transformation. It has also been found that this protein interacts with the E2 protein of human papilloma virus type 16 (HPV16). The oncogenic HPV forms, such as HPV16, are known to be necessary but not sufficient agents in the development of cervical carcinoma. In the present study, the CCHCR1 gene coding sequence and its expression was analyzed in normal, precancerous and cervical cancer cells. Changes in the non-coding region were found in 20.3% of the examined probes from women with cervical cancer or precancerous lesions and in 16.67% of the control probes. Most of the detected changes were single nucleotide polymorphisms (SNPs). Changes in the coding region were found in 22.8% of the probes with cervical cancer and in 16.67% of the control probes and all of them were SNPs. The level of CCHCR1 transcripts was determined using the real-time PCR method and the highest gene expression was detected in the H-SIL group and slightly decreased in the cervical carcinoma cells, compared with the control probes. It suggests that CCHCR1 could have a role in the process of cervical epithelial cell transformation, but this suggestion must be confirmed experimentally.
Department of Animal Physiology and Development, Adam Mickiewicz University, ul. Umultowska 89, 61-614 Poznan, Poland. pacholsk@amu.edu.pl
Joanna
J
Pacholska-Bogalska
Magdalena
M
Myga-Nowak
Katarzyna
K
Ciepłuch
Agata
A
Józefiak
Anna
A
Kwaśniewska
Anna
A
Goździcka-Józefiak
D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D015603:Keratinocytes / Q000473:pathology / Q000821:virology*; D016366:Open Reading Frames; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
C412616:CCHCR1 protein, human; D047908:Intracellular Signaling Peptides and Proteins