Screening and identification of antimicrobial compounds from Streptomyces bottropensis suppressing rice bacterial blight.
Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating pathogen to Oryza sativa and has been shown to cause bacterial blight. Two bioactive compounds showing antimicrobial activities against Xoo strain KACC 10331 were isolated from a Streptomyces bottropensis strain. The ethyl acetate extract was fractionated on a Sephadex LH-20 column, and then purified by preparative HPLC. The purified compounds were identified as bottromycin A2 and dunaimycin D3S by HR/MS and 1H NMR analyses. The MIC value against Xoo and the lowest concentration still capable of suppressing rice bacterial blight were 2 microgram/ml and 16 microgram/ml for bottromycin A2, and 64 microgram/ml and 0.06 microgram/ml for dunaimycin D3S, respectively. These two compounds were shown to exert different bioactivities in vitro and in rice leaf explants.
Division of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
Sait Byul
SB
Park
In Ae
IA
Lee
Joo-Won
JW
Suh
Jeong-Gu
JG
Kim
Choong Hwan
CH
Lee
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D015536:Down-Regulation; D004353:Drug Evaluation, Preclinical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*; D014974:Xanthomonas / Q000187:drug effects* / Q000502:physiology
D000900:Anti-Bacterial Agents