Poly(ethylene glycol)-coated microfluidic devices for chip electrophoresis.
Herein, we report on a strategy for durable modification of the channel surface in microfluidic glass chips with the neutral hydrophilic-coating material poly(ethylene glycol) PEG-1M-100. Applied in microchip electrophoresis such PEG-coated devices exhibit a suppressed electroosmotic flow and reduced analyte adsorption. The PEG-coated chips were successfully applied in chip electrophoresis of FITC-labelled amines and amino acids and native proteins as well as in chiral separations. The performance of the coated chips was found to be superior compared with uncoated microchips. The coated chips exhibited high stability and the relative standard deviation of migration times in PEG-coated devices was less than 2%.
University of Leipzig, Institute of Analytical Chemistry, Leipzig, Germany.
Marcel
M
Schulze
Detlev
D
Belder
D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification; D053841:Electroosmosis; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013499:Surface Properties
D000596:Amino Acids; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate