BVPaP-3, a T7-like lytic phage of Pseudomonas aeruginosa: its isolation and characterisation.
The increasing emergence of antibiotic-resistant bacteria has produced a growing interest among scientists in bacteriophages as alternative antimicrobial agents. This article reports a lytic phage against an antibiotic-resistant strain of Pseudomonas aeruginosa. Phage BVPaP-3 is a member of the Podoviridae family and morphologically similar to the T7-like phage gh-1. The phage has a hexagonal head of 58-59 nm in diameter and a short tail of 10 × 8 nm. It is stable at a wide range of pH (6-10) and temperatures (4-40°C). Its optimal growth temperature is 37°C and the adsorption rate constant is 1.19 × 10(-9). Latent and eclipse periods are 20 and 15 min, respectively, and the burst size is 44 after 35 min at 37°C. The phage has a DNA size of 41.31 kb and a proteome of 11 proteins. The major protein is 33 kDa in size.
Department of Environmental Science, University of Pune, Pune, India.
Sangeeta
S
Ahiwale
Divya
D
Prakash
Milind
M
Gajbhiye
Smita
S
Jagdale
Nita
N
Patil
Balu
B
Kapadnis
D004279:DNA, Viral / Q000235:genetics; D058507:Host Specificity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D008970:Molecular Weight; D017902:Podoviridae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D020543:Proteome / Q000032:analysis; D017105:Pseudomonas Phages / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000821:virology*; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis / Q000737:chemistry; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D020543:Proteome; D014764:Viral Proteins