Transcriptome analysis of an oil-rich race B strain of Botryococcus braunii (BOT-70) by de novo assembly of 5'-end sequences of full-length cDNA clones.
Here the transcriptome of an oil-rich race B strain of Botryococcus braunii (BOT-70) was analyzed to mine genetic information useful in biofuel development. A full-length-enriched cDNA library was constructed via the oligo-capping method and the 5' ends of 11,904 randomly chosen cDNA clones were sequenced. Homology search using BLASTX identified candidate BOT-70 genes for majority of the reactions required for biosynthesis of botryococcenes through the mevalonate-independent pathway. The sequence retrieval from the transcriptome dataset implicated that an alternative entry route into the mevalonate-independent pathway via xylulose-5-phosphate, rather than the conventional entry route via 1-deoxy-d-xylulose-5-phosphate, is predominantly active. Analysis of N-terminal sequences of the retrieved genes indicated that the final reactions of botryococcene biosynthesis are likely to take place outside of chloroplasts. The transcriptome dataset has been deposited in the GenBank/EMBL/DDBJ database.
Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
Motohide
M
Ioki
Masato
M
Baba
Nobuyoshi
N
Nakajima
Yoshihiro
Y
Shiraiwa
Makoto M
MM
Watanabe
D001483:Base Sequence; D000460:Chlorophyta / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D059467:Transcriptome / Q000235:genetics*
D018076:DNA, Complementary; D010938:Plant Oils