Enhanced percutaneous delivery of recombinant human epidermal growth factor employing nano-liposome system.
We encapsulated recombinant human epidermal growth factor (rhEGF) into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoViva™). Particle size, zeta (ζ) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, zeta potential and encapsulation efficiency of the NLs were 155.57 ± 2.59 nm, -57.92 ± 4.35 mV and 9.00 ± 0.39%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin.
Department of Smart Foods and Drugs, Inje University, 607 Obang-dong, Gimhae, Gyeongnam 621-749, Korea.
Sang-Ok
SO
Jeon
Hee-Jin
HJ
Hwang
Dong-Ho
DH
Oh
Jo-Eun
JE
Seo
Kyeung-Hwa
KH
Chun
Sun-Mi
SM
Hong
Min-Ju
MJ
Kim
Won-Chul
WC
Kim
Min-Sun
MS
Park
Chae-Ha
CH
Yoon
Kyung-Hyun
KH
Min
Chang-Woo
CW
Suh
Sangkil
S
Lee
D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004058:Diffusion; D016503:Drug Delivery Systems; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000378:metabolism
D008081:Liposomes; D004815:Epidermal Growth Factor