Development of a quartz crystal microbalance biosensor with aptamers as bio-recognition element.
The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10-240 μg/L) compared to that of the aptamer (2.5-200 μg/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. yao_yao24@yahoo.com
Chunyan
C
Yao
Tangyou
T
Zhu
Yongzhi
Y
Qi
Yuhui
Y
Zhao
Han
H
Xia
Weiling
W
Fu
D000918:Antibody Specificity; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000378:metabolism; D009994:Osmolar Concentration; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation*; D013379:Substrate Specificity
D052157:Aptamers, Nucleotide; D007073:Immunoglobulin E