WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain.
WRNIP1 (Werner helicase-interacting protein 1) was originally identified as a protein that interacts with the Werner syndrome responsible gene product. WRNIP1 contains a ubiquitin-binding zinc-finger (UBZ) domain in the N-terminal region and two leucine zipper motifs in the C-terminal region. In addition, it possesses an ATPase domain in the middle of the molecule and the lysine residues serving as ubiquitin acceptors in the entire of the molecule. Here, we report that WRNIP1 accumulates in laser light irradiated sites very rapidly via its ubiquitin-binding zinc finger domain, which is known to bind polyubiquitin and to be involved in ubiquitination of WRNIP1 itself. The accumulation of WRNIP1 in laser light irradiated sites also required the C-terminal region containing two leucine zippers, which is reportedly involved in the oligomerization of WRNIP1. Mutated WRNIP1 with a deleted ATPase domain or with mutations in lysine residues, which serve as ubiquitin acceptors, accumulated in laser light irradiated sites, suggesting that the ATPase domain of WRNIP1 and ubiquitination of WRNIP1 are dispensable for the accumulation.
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki Aoba 6-3, Aobaku, Sendai 980-8578, Japan.
Hironoshin
H
Nomura
Akari
A
Yoshimura
Takato
T
Edo
Shin-ichiro
S
Kanno
Syusuke
S
Tada
Masayuki
M
Seki
Akira
A
Yasui
Takemi
T
Enomoto
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D016350:Leucine Zippers; D008027:Light; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D017434:Protein Structure, Tertiary; D025801:Ubiquitin / Q000378:metabolism*; D054875:Ubiquitination; D016335:Zinc Fingers
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D025801:Ubiquitin; D000251:Adenosine Triphosphatases; C430237:WRNIP1 protein, human; D000074183:ATPases Associated with Diverse Cellular Activities; D008239:Lysine