Antioxidant capacities and total polyphenol contents of hydro-ethanolic extract of phytococktail from trans-Himalaya.
The aim of this study was to evaluate the in vitro antioxidant potential of hydro-ethanolic extract of a novel phytococktail comprising of sea buckthorn, apricot, and Rhodiola (SAR) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) activity of the extract increased in a dose-dependent manner (upto 0.1 mg/mL), and was found to be about 38% of that of ascorbic acid at 0.1 mg/mL. The hydro-ethanolic extract of SAR also scavenged the ABTS(.+)  radical generated by ABTS/potassium persulfate (PPS) system and was found to be about 62% of that of ascorbic acid at 0.1 mg/ mL. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total phenolic content was found to be 1.28016 × 10(-3)  mol gallic acid equivalent (GAE)/g extract. Total flavonoid and flavonol contents were estimated to be 2.5970 × 10(-4)  mol and 4.87 × 10(-4)  mol quercetin equivalent/g extract, respectively. The hydro-ethanolic extract of this phytococktail indicated presence of essential phytoconstituents of polyphenols, flavonoids, flavonols, and ascorbic acid, which contributed significantly to its antioxidant capacity. The combination of the 3 plants may well support their use in traditional medicine to combat oxidative stress and high-altitude sickness.
Defence Inst of High Altitude Research, Defence Research & Development Organisation, Leh-Ladakh 901205, India.
P
P
Dhar
A B
AB
Tayade
P K
PK
Bajpai
V K
VK
Sharma
S K
SK
Das
O P
OP
Chaurasia
R B
RB
Srivastava
S B
SB
Singh
D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D052160:Benzothiazoles / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005707:Gallic Acid / Q000378:metabolism; D031281:Hippophae / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry; D013451:Sulfonic Acids / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D010936:Plant Extracts; D059808:Polyphenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; D005707:Gallic Acid; D001205:Ascorbic Acid