Lactobacillus paracasei Lp6 favors immune modulation induced by allergoid treatment in ragweed sensitized mice.
It has been hypothesized that lactic acid bacteria (LAB) could be used as adjuvant for specific immunotherapy (SIT), as various studies conducted on humans and animals converge to define LAB  as anti-Th2 modulators and Treg inducers. In the present study we evaluated the effects of LAB, in particular Lactobacillus paracasei Lp6 (Lp6), in a mouse model of ragweed (RW) allergy. Groups of Balb/c mice, experimentally sensitized towards ragweed, were treated by viable Lp6 or by RWallergoid with or without co-administration of Lp6. A control group was sham-sensitized with PBS and sham-treated with water and a group was sensitized with RW and treated with water. Serum IgE, RW-induced release of IFN-gamma, IL-4 and IL-10 from splenocytes and the frequency of CD4CD25 regulatory T cells (Tregs) expressing Foxp3 or IL-10 were evaluated in various groups. RW-allergoid treatment induced a reduction of serum IgE, with a decrease in RW-induced release of IL-4, and an increase in IL-10 and IFN-gamma, along with a significant change in the frequency of Tregs, both CD25+ and -. The joint RWallergoid+ Lp6 treatment induced the highest degree of suppression of allergen-driven IL-4, the greatest reduction of IL-4/IFN-gamma and IL-4/IL-10 ratios and the most significant increase of Foxp3 and IL-10 expressing Tregs. The study shows that Lp6 strengthens the immune modulation induced by allergoid-SIT in RW-sensitized mice, essentially characterized by a differential induction of Tregs associated to a reduction of IL-4; data converge to define a role of SIT adjuvant for Lp6.
Center for Ageing Sciences, University of Chieti Foundation, Chieti, Italy.
C
C
Petrarca
F
F
Lazzarin
P
P
Lanuti
M
M
Marchisio
S
S
Miscia
C
C
Rossi
M
M
Braga
G
G
Mistrello
M
M
Di Gioacchino
D000276:Adjuvants, Immunologic; D000080384:Allergoids; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002478:Cells, Cultured; D003888:Desensitization, Immunologic; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007778:Lactobacillus / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000276:Adjuvants, Immunologic; D000080384:Allergoids; C026967:Amb a I protein, Ambrosia artemisiifolia; D052179:Antigens, Plant; D001425:Bacterial Outer Membrane Proteins; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D008074:Lipoproteins; D010936:Plant Extracts; D010940:Plant Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma